Mme cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mme
Entrez Gene ID	17380
Full Name	membrane metallo endopeptidase
Synonyms	6030454K05Rik,C85356,CALLA,CD10,NEP,SFE
General protein information	Preferred Names membrane metallo endopeptidase Names neprilysin atriopeptidase common acute lymphoblastic leukemia antigen enkephalinase neutral endopeptidase 24.11 skin fibroblast elastase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 E1\|3 29.97 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008604.4	NP_032630.2	neprilysin
XM_006501095.3	XP_006501158.1	neprilysin isoform X1
NM_001357335.1	NP_001344264.1	neprilysin
NM_001289462.1	NP_001276391.1	neprilysin
NM_001289463.1	NP_001276392.1	neprilysin
XM_006501098.3	XP_006501161.1	neprilysin isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MME	NP_001179813.1
Homo sapiens (human)	MME	NP_009220.2
Pan troglodytes (chimpanzee)	MME	XP_001148532.1
Canis lupus familiaris (dog)	MME	XP_005634647.1
Gallus gallus (chicken)	MME	NP_001004412.1
	MME	XP_001105907.1
Mus musculus (house mouse)	Mme	NP_032630.2
Rattus norvegicus (Norway rat)	Mme	NP_036740.1

Gene Ontology
Bibliography

Related articles in PubMed

Biochemistry, Therapeutics, and Biomarker Implications of Neprilysin in Cardiorenal Disease.
Chen Y, Burnett JC Jr
Clinical chemistry63(1)108-115(2017 Jan)

A Test in Context: Neprilysin: Function, Inhibition, and Biomarker.
Bayes-Genis A, Barallat J, Richards AM
Journal of the American College of Cardiology68(6)639-53(2016 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results indicate that NEP accelerates adipogenesis through enhancement of insulin-mediated PI3K-Akt activation and imply a high therapeutic value of NEP in treating obesity and obesity-related disorders.
Title: Neprilysin facilitates adipogenesis through potentiation of the phosphatidylinositol 3-kinase (PI3K) signaling pathway.

Rare Variants in MME, Encoding Metalloprotease Neprilysin, Are Linked to Late-Onset Autosomal-Dominant Axonal Polyneuropathies
Title: Rare Variants in MME, Encoding Metalloprotease Neprilysin, Are Linked to Late-Onset Autosomal-Dominant Axonal Polyneuropathies.

Amyloid beta (Abeta)-degrading effects of overexpressed neprilysin can block deleterious BACE1-elevating mechanisms that accelerate Abeta production
Title: A combination Alzheimer's therapy targeting BACE1 and neprilysin in 5XFAD transgenic mice.

Serum CD10 levels might serve as a useful marker of synchronous and metachronous liver metastasis in colorectal cancer.
Title: Serum CD10 is associated with liver metastasis in colorectal cancer.

Data indicate that 5-aza-deoxycytidine induced the demethylation of neprilysin (NEP) gene and significantly increased NEP expression in a dose-dependent manner.
Title: [Expression of neprilysin gene is associated with methylation and histone modification on promoter in mouse neuroblastoma Neuro-2a cells].

The following Mme gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mme cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23141	NM_001357335.1 Latest version!	Mus musculus membrane metallo endopeptidase (Mme), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu23141	NM_001289463.1 Latest version!	Mus musculus membrane metallo endopeptidase (Mme), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu23141	NM_001289462.1 Latest version!	Mus musculus membrane metallo endopeptidase (Mme), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu23141	NM_008604.4 Latest version!	Mus musculus membrane metallo endopeptidase (Mme), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu23141	XM_006501095.3 Latest version!	Mus musculus membrane metallo endopeptidase (Mme), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu23141	XM_006501098.3 Latest version!	Mus musculus membrane metallo endopeptidase (Mme), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mme ( NM_008604.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032630.2 Mus musculus membrane metallo endopeptidase (Mme), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23141
Clone ID Related Accession (Same CDS sequence)	NM_008604.4 , XM_006501095.3 , NM_001357335.1 , NM_001289462.1 , NM_001289463.1 , XM_006501098.3
Accession Version	NM_008604.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	neprilysin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK031446.1 and AC121840.3. On Jan 22, 2014 this sequence version replaced NM_008604.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031446.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ATGCTCCAAA GCCAAAGAAG 
AAACAGCGAT GGACTCCCCT GGAGATCAGC CTCTCTGTGC TTGTCTTGCT CCTGACTATC 
ATAGCGGTGA CAATGATCGC TCTCTATGCA ACCTATGATG ATGGCATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATT CAGAATATGG ATGCCTCTGT TGAGCCATGT 
ACAGACTTCT TCAAGTATGC TTGTGGAGGC TGGTTGAAAC GCAACGTCAT TCCCGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTTTA AGAGATGAGC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAAGACATA GTAGCAGTGC AGAAAGCAAA AACCTTGTAC 
AGATCGTGTA TAAATGAGTC TGCTATTGAT AGCAGAGGCG GACAACCTCT ACTCAAACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCAGATAACT GGGATCAAAC ATATGGCACT 
TCTTGGACAG CTGAGAAATC CATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GCACTGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGTTTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCTAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTGCAGAAC AACTTTTCCC TGGAGGTCAA TGGGAAGTCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TTGTTGTCTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTATGGG 
ACTACATCAG AAACTGCAAC ATGGAGACGG TGTGCTAACT ACGTCAATGG GAATATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCG GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGACTTGA TTGCACAAAT CCGGGAAGTT TTTATTCAGA CTTTGGATGA CCTTACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAAAAGGCCT TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT TTCCAATGAG AACAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAGGG AAGATGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAAGTG GACAAAGATG AGTGGATAAG TGGAGCAGCT 
GTAGTCAATG CATTTTATTC CTCAGGCCGA AATCAGATAG TCTTCCCCGC TGGCATTTTG 
CAGCCCCCAT TCTTCAGTGC TCAGCAATCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATTGGAC ATGAAATCAC ACATGGATTT GATGACAATG GAAGAAATTT CAATAAAGAT 
GGAGACCTCG TTGACTGGTG GACGCAGCAG TCGGCAAATA ATTTTAAAGA CCAATCCCAG 
TGTATGGTAT ACCAGTATGG AAACTTTTCC TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAATA CACTAGGAGA AAACATTGCT GATAATGGAG GTATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTTTT CCTGAACTTT GCCCAGGTGT GGTGTGGGAC CTACCGGCCA 
GAGTATGCAG TCAATTCTAT TAAAACAGAT GTACACAGTC CTGGAAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC CGAGTTTGCA GATGCCTTTC ATTGCCGCAA GAATTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032630.2
CDS	89..2341
Translation	MGRSESQMDITDINAPKPKKKQRWTPLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDASVEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLKLLPDIYGWPVASDNWDQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQSLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEVNGKSFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYREDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

Target ORF information:

RefSeq Version	NM_008604.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane metallo endopeptidase (Mme), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008604.4

ORF Insert Sequence:

ATGGGAAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ATGCTCCAAA GCCAAAGAAG 
AAACAGCGAT GGACTCCCCT GGAGATCAGC CTCTCTGTGC TTGTCTTGCT CCTGACTATC 
ATAGCGGTGA CAATGATCGC TCTCTATGCA ACCTATGATG ATGGCATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATT CAGAATATGG ATGCCTCTGT TGAGCCATGT 
ACAGACTTCT TCAAGTATGC TTGTGGAGGC TGGTTGAAAC GCAACGTCAT TCCCGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTTTA AGAGATGAGC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAAGACATA GTAGCAGTGC AGAAAGCAAA AACCTTGTAC 
AGATCGTGTA TAAATGAGTC TGCTATTGAT AGCAGAGGCG GACAACCTCT ACTCAAACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCAGATAACT GGGATCAAAC ATATGGCACT 
TCTTGGACAG CTGAGAAATC CATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GCACTGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGTTTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCTAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTGCAGAAC AACTTTTCCC TGGAGGTCAA TGGGAAGTCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TTGTTGTCTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTATGGG 
ACTACATCAG AAACTGCAAC ATGGAGACGG TGTGCTAACT ACGTCAATGG GAATATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCG GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGACTTGA TTGCACAAAT CCGGGAAGTT TTTATTCAGA CTTTGGATGA CCTTACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAAAAGGCCT TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT TTCCAATGAG AACAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAGGG AAGATGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAAGTG GACAAAGATG AGTGGATAAG TGGAGCAGCT 
GTAGTCAATG CATTTTATTC CTCAGGCCGA AATCAGATAG TCTTCCCCGC TGGCATTTTG 
CAGCCCCCAT TCTTCAGTGC TCAGCAATCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATTGGAC ATGAAATCAC ACATGGATTT GATGACAATG GAAGAAATTT CAATAAAGAT 
GGAGACCTCG TTGACTGGTG GACGCAGCAG TCGGCAAATA ATTTTAAAGA CCAATCCCAG 
TGTATGGTAT ACCAGTATGG AAACTTTTCC TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAATA CACTAGGAGA AAACATTGCT GATAATGGAG GTATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTTTT CCTGAACTTT GCCCAGGTGT GGTGTGGGAC CTACCGGCCA 
GAGTATGCAG TCAATTCTAT TAAAACAGAT GTACACAGTC CTGGAAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC CGAGTTTGCA GATGCCTTTC ATTGCCGCAA GAATTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mme ( NM_001357335.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344264.1 Mus musculus membrane metallo endopeptidase (Mme), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu23141
Clone ID Related Accession (Same CDS sequence)	NM_008604.4 , XM_006501095.3 , NM_001357335.1 , NM_001289462.1 , NM_001289463.1 , XM_006501098.3
Accession Version	NM_001357335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	neprilysin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121993.2 and AC121840.3. On Oct 26, 2017 this sequence version replaced XM_006501095.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.9489.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ATGCTCCAAA GCCAAAGAAG 
AAACAGCGAT GGACTCCCCT GGAGATCAGC CTCTCTGTGC TTGTCTTGCT CCTGACTATC 
ATAGCGGTGA CAATGATCGC TCTCTATGCA ACCTATGATG ATGGCATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATT CAGAATATGG ATGCCTCTGT TGAGCCATGT 
ACAGACTTCT TCAAGTATGC TTGTGGAGGC TGGTTGAAAC GCAACGTCAT TCCCGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTTTA AGAGATGAGC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAAGACATA GTAGCAGTGC AGAAAGCAAA AACCTTGTAC 
AGATCGTGTA TAAATGAGTC TGCTATTGAT AGCAGAGGCG GACAACCTCT ACTCAAACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCAGATAACT GGGATCAAAC ATATGGCACT 
TCTTGGACAG CTGAGAAATC CATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GCACTGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGTTTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCTAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTGCAGAAC AACTTTTCCC TGGAGGTCAA TGGGAAGTCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TTGTTGTCTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTATGGG 
ACTACATCAG AAACTGCAAC ATGGAGACGG TGTGCTAACT ACGTCAATGG GAATATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCG GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGACTTGA TTGCACAAAT CCGGGAAGTT TTTATTCAGA CTTTGGATGA CCTTACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAAAAGGCCT TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT TTCCAATGAG AACAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAGGG AAGATGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAAGTG GACAAAGATG AGTGGATAAG TGGAGCAGCT 
GTAGTCAATG CATTTTATTC CTCAGGCCGA AATCAGATAG TCTTCCCCGC TGGCATTTTG 
CAGCCCCCAT TCTTCAGTGC TCAGCAATCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATTGGAC ATGAAATCAC ACATGGATTT GATGACAATG GAAGAAATTT CAATAAAGAT 
GGAGACCTCG TTGACTGGTG GACGCAGCAG TCGGCAAATA ATTTTAAAGA CCAATCCCAG 
TGTATGGTAT ACCAGTATGG AAACTTTTCC TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAATA CACTAGGAGA AAACATTGCT GATAATGGAG GTATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTTTT CCTGAACTTT GCCCAGGTGT GGTGTGGGAC CTACCGGCCA 
GAGTATGCAG TCAATTCTAT TAAAACAGAT GTACACAGTC CTGGAAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC CGAGTTTGCA GATGCCTTTC ATTGCCGCAA GAATTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344264.1
CDS	416..2668
Translation	MGRSESQMDITDINAPKPKKKQRWTPLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDASVEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLKLLPDIYGWPVASDNWDQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQSLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEVNGKSFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYREDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

Target ORF information:

RefSeq Version	NM_001357335.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane metallo endopeptidase (Mme), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357335.1

ORF Insert Sequence:

ATGGGAAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ATGCTCCAAA GCCAAAGAAG 
AAACAGCGAT GGACTCCCCT GGAGATCAGC CTCTCTGTGC TTGTCTTGCT CCTGACTATC 
ATAGCGGTGA CAATGATCGC TCTCTATGCA ACCTATGATG ATGGCATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATT CAGAATATGG ATGCCTCTGT TGAGCCATGT 
ACAGACTTCT TCAAGTATGC TTGTGGAGGC TGGTTGAAAC GCAACGTCAT TCCCGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTTTA AGAGATGAGC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAAGACATA GTAGCAGTGC AGAAAGCAAA AACCTTGTAC 
AGATCGTGTA TAAATGAGTC TGCTATTGAT AGCAGAGGCG GACAACCTCT ACTCAAACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCAGATAACT GGGATCAAAC ATATGGCACT 
TCTTGGACAG CTGAGAAATC CATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GCACTGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGTTTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCTAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTGCAGAAC AACTTTTCCC TGGAGGTCAA TGGGAAGTCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TTGTTGTCTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTATGGG 
ACTACATCAG AAACTGCAAC ATGGAGACGG TGTGCTAACT ACGTCAATGG GAATATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCG GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGACTTGA TTGCACAAAT CCGGGAAGTT TTTATTCAGA CTTTGGATGA CCTTACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAAAAGGCCT TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT TTCCAATGAG AACAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAGGG AAGATGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAAGTG GACAAAGATG AGTGGATAAG TGGAGCAGCT 
GTAGTCAATG CATTTTATTC CTCAGGCCGA AATCAGATAG TCTTCCCCGC TGGCATTTTG 
CAGCCCCCAT TCTTCAGTGC TCAGCAATCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATTGGAC ATGAAATCAC ACATGGATTT GATGACAATG GAAGAAATTT CAATAAAGAT 
GGAGACCTCG TTGACTGGTG GACGCAGCAG TCGGCAAATA ATTTTAAAGA CCAATCCCAG 
TGTATGGTAT ACCAGTATGG AAACTTTTCC TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAATA CACTAGGAGA AAACATTGCT GATAATGGAG GTATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTTTT CCTGAACTTT GCCCAGGTGT GGTGTGGGAC CTACCGGCCA 
GAGTATGCAG TCAATTCTAT TAAAACAGAT GTACACAGTC CTGGAAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC CGAGTTTGCA GATGCCTTTC ATTGCCGCAA GAATTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mme ( NM_001289462.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276391.1 Mus musculus membrane metallo endopeptidase (Mme), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23141
Clone ID Related Accession (Same CDS sequence)	NM_008604.4 , XM_006501095.3 , NM_001357335.1 , NM_001289462.1 , NM_001289463.1 , XM_006501098.3
Accession Version	NM_001289462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	neprilysin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY145991.1, AK076908.1, AK031446.1 and AC121840.3. On Jan 22, 2014 this sequence version replaced XM_006501097.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK076908.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ATGCTCCAAA GCCAAAGAAG 
AAACAGCGAT GGACTCCCCT GGAGATCAGC CTCTCTGTGC TTGTCTTGCT CCTGACTATC 
ATAGCGGTGA CAATGATCGC TCTCTATGCA ACCTATGATG ATGGCATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATT CAGAATATGG ATGCCTCTGT TGAGCCATGT 
ACAGACTTCT TCAAGTATGC TTGTGGAGGC TGGTTGAAAC GCAACGTCAT TCCCGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTTTA AGAGATGAGC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAAGACATA GTAGCAGTGC AGAAAGCAAA AACCTTGTAC 
AGATCGTGTA TAAATGAGTC TGCTATTGAT AGCAGAGGCG GACAACCTCT ACTCAAACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCAGATAACT GGGATCAAAC ATATGGCACT 
TCTTGGACAG CTGAGAAATC CATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GCACTGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGTTTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCTAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTGCAGAAC AACTTTTCCC TGGAGGTCAA TGGGAAGTCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TTGTTGTCTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTATGGG 
ACTACATCAG AAACTGCAAC ATGGAGACGG TGTGCTAACT ACGTCAATGG GAATATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCG GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGACTTGA TTGCACAAAT CCGGGAAGTT TTTATTCAGA CTTTGGATGA CCTTACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAAAAGGCCT TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT TTCCAATGAG AACAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAGGG AAGATGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAAGTG GACAAAGATG AGTGGATAAG TGGAGCAGCT 
GTAGTCAATG CATTTTATTC CTCAGGCCGA AATCAGATAG TCTTCCCCGC TGGCATTTTG 
CAGCCCCCAT TCTTCAGTGC TCAGCAATCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATTGGAC ATGAAATCAC ACATGGATTT GATGACAATG GAAGAAATTT CAATAAAGAT 
GGAGACCTCG TTGACTGGTG GACGCAGCAG TCGGCAAATA ATTTTAAAGA CCAATCCCAG 
TGTATGGTAT ACCAGTATGG AAACTTTTCC TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAATA CACTAGGAGA AAACATTGCT GATAATGGAG GTATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTTTT CCTGAACTTT GCCCAGGTGT GGTGTGGGAC CTACCGGCCA 
GAGTATGCAG TCAATTCTAT TAAAACAGAT GTACACAGTC CTGGAAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC CGAGTTTGCA GATGCCTTTC ATTGCCGCAA GAATTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276391.1
CDS	455..2707
Translation	MGRSESQMDITDINAPKPKKKQRWTPLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDASVEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLKLLPDIYGWPVASDNWDQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQSLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEVNGKSFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYREDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

Target ORF information:

RefSeq Version	NM_001289462.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane metallo endopeptidase (Mme), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289462.1

ORF Insert Sequence:

ATGGGAAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ATGCTCCAAA GCCAAAGAAG 
AAACAGCGAT GGACTCCCCT GGAGATCAGC CTCTCTGTGC TTGTCTTGCT CCTGACTATC 
ATAGCGGTGA CAATGATCGC TCTCTATGCA ACCTATGATG ATGGCATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATT CAGAATATGG ATGCCTCTGT TGAGCCATGT 
ACAGACTTCT TCAAGTATGC TTGTGGAGGC TGGTTGAAAC GCAACGTCAT TCCCGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTTTA AGAGATGAGC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAAGACATA GTAGCAGTGC AGAAAGCAAA AACCTTGTAC 
AGATCGTGTA TAAATGAGTC TGCTATTGAT AGCAGAGGCG GACAACCTCT ACTCAAACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCAGATAACT GGGATCAAAC ATATGGCACT 
TCTTGGACAG CTGAGAAATC CATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GCACTGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGTTTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCTAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTGCAGAAC AACTTTTCCC TGGAGGTCAA TGGGAAGTCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TTGTTGTCTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTATGGG 
ACTACATCAG AAACTGCAAC ATGGAGACGG TGTGCTAACT ACGTCAATGG GAATATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCG GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGACTTGA TTGCACAAAT CCGGGAAGTT TTTATTCAGA CTTTGGATGA CCTTACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAAAAGGCCT TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT TTCCAATGAG AACAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAGGG AAGATGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAAGTG GACAAAGATG AGTGGATAAG TGGAGCAGCT 
GTAGTCAATG CATTTTATTC CTCAGGCCGA AATCAGATAG TCTTCCCCGC TGGCATTTTG 
CAGCCCCCAT TCTTCAGTGC TCAGCAATCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATTGGAC ATGAAATCAC ACATGGATTT GATGACAATG GAAGAAATTT CAATAAAGAT 
GGAGACCTCG TTGACTGGTG GACGCAGCAG TCGGCAAATA ATTTTAAAGA CCAATCCCAG 
TGTATGGTAT ACCAGTATGG AAACTTTTCC TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAATA CACTAGGAGA AAACATTGCT GATAATGGAG GTATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTTTT CCTGAACTTT GCCCAGGTGT GGTGTGGGAC CTACCGGCCA 
GAGTATGCAG TCAATTCTAT TAAAACAGAT GTACACAGTC CTGGAAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC CGAGTTTGCA GATGCCTTTC ATTGCCGCAA GAATTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mme ( NM_001289463.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276392.1 Mus musculus membrane metallo endopeptidase (Mme), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu23141
Clone ID Related Accession (Same CDS sequence)	NM_008604.4 , XM_006501095.3 , NM_001357335.1 , NM_001289462.1 , NM_001289463.1 , XM_006501098.3
Accession Version	NM_001289463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	neprilysin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121993.2, AK033824.1, AK031446.1 and AC121840.3. On Jan 22, 2014 this sequence version replaced XM_006501096.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK033824.1, BC034092.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ATGCTCCAAA GCCAAAGAAG 
AAACAGCGAT GGACTCCCCT GGAGATCAGC CTCTCTGTGC TTGTCTTGCT CCTGACTATC 
ATAGCGGTGA CAATGATCGC TCTCTATGCA ACCTATGATG ATGGCATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATT CAGAATATGG ATGCCTCTGT TGAGCCATGT 
ACAGACTTCT TCAAGTATGC TTGTGGAGGC TGGTTGAAAC GCAACGTCAT TCCCGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTTTA AGAGATGAGC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAAGACATA GTAGCAGTGC AGAAAGCAAA AACCTTGTAC 
AGATCGTGTA TAAATGAGTC TGCTATTGAT AGCAGAGGCG GACAACCTCT ACTCAAACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCAGATAACT GGGATCAAAC ATATGGCACT 
TCTTGGACAG CTGAGAAATC CATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GCACTGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGTTTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCTAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTGCAGAAC AACTTTTCCC TGGAGGTCAA TGGGAAGTCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TTGTTGTCTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTATGGG 
ACTACATCAG AAACTGCAAC ATGGAGACGG TGTGCTAACT ACGTCAATGG GAATATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCG GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGACTTGA TTGCACAAAT CCGGGAAGTT TTTATTCAGA CTTTGGATGA CCTTACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAAAAGGCCT TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT TTCCAATGAG AACAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAGGG AAGATGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAAGTG GACAAAGATG AGTGGATAAG TGGAGCAGCT 
GTAGTCAATG CATTTTATTC CTCAGGCCGA AATCAGATAG TCTTCCCCGC TGGCATTTTG 
CAGCCCCCAT TCTTCAGTGC TCAGCAATCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATTGGAC ATGAAATCAC ACATGGATTT GATGACAATG GAAGAAATTT CAATAAAGAT 
GGAGACCTCG TTGACTGGTG GACGCAGCAG TCGGCAAATA ATTTTAAAGA CCAATCCCAG 
TGTATGGTAT ACCAGTATGG AAACTTTTCC TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAATA CACTAGGAGA AAACATTGCT GATAATGGAG GTATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTTTT CCTGAACTTT GCCCAGGTGT GGTGTGGGAC CTACCGGCCA 
GAGTATGCAG TCAATTCTAT TAAAACAGAT GTACACAGTC CTGGAAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC CGAGTTTGCA GATGCCTTTC ATTGCCGCAA GAATTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276392.1
CDS	239..2491
Translation	MGRSESQMDITDINAPKPKKKQRWTPLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDASVEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLKLLPDIYGWPVASDNWDQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQSLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEVNGKSFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYREDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

Target ORF information:

RefSeq Version	NM_001289463.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane metallo endopeptidase (Mme), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289463.1

ORF Insert Sequence:

ATGGGAAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ATGCTCCAAA GCCAAAGAAG 
AAACAGCGAT GGACTCCCCT GGAGATCAGC CTCTCTGTGC TTGTCTTGCT CCTGACTATC 
ATAGCGGTGA CAATGATCGC TCTCTATGCA ACCTATGATG ATGGCATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATT CAGAATATGG ATGCCTCTGT TGAGCCATGT 
ACAGACTTCT TCAAGTATGC TTGTGGAGGC TGGTTGAAAC GCAACGTCAT TCCCGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTTTA AGAGATGAGC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAAGACATA GTAGCAGTGC AGAAAGCAAA AACCTTGTAC 
AGATCGTGTA TAAATGAGTC TGCTATTGAT AGCAGAGGCG GACAACCTCT ACTCAAACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCAGATAACT GGGATCAAAC ATATGGCACT 
TCTTGGACAG CTGAGAAATC CATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GCACTGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGTTTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCTAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTGCAGAAC AACTTTTCCC TGGAGGTCAA TGGGAAGTCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TTGTTGTCTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTATGGG 
ACTACATCAG AAACTGCAAC ATGGAGACGG TGTGCTAACT ACGTCAATGG GAATATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCG GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGACTTGA TTGCACAAAT CCGGGAAGTT TTTATTCAGA CTTTGGATGA CCTTACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAAAAGGCCT TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT TTCCAATGAG AACAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAGGG AAGATGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAAGTG GACAAAGATG AGTGGATAAG TGGAGCAGCT 
GTAGTCAATG CATTTTATTC CTCAGGCCGA AATCAGATAG TCTTCCCCGC TGGCATTTTG 
CAGCCCCCAT TCTTCAGTGC TCAGCAATCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATTGGAC ATGAAATCAC ACATGGATTT GATGACAATG GAAGAAATTT CAATAAAGAT 
GGAGACCTCG TTGACTGGTG GACGCAGCAG TCGGCAAATA ATTTTAAAGA CCAATCCCAG 
TGTATGGTAT ACCAGTATGG AAACTTTTCC TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAATA CACTAGGAGA AAACATTGCT GATAATGGAG GTATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTTTT CCTGAACTTT GCCCAGGTGT GGTGTGGGAC CTACCGGCCA 
GAGTATGCAG TCAATTCTAT TAAAACAGAT GTACACAGTC CTGGAAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC CGAGTTTGCA GATGCCTTTC ATTGCCGCAA GAATTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mme ( XM_006501095.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501158.1 Mus musculus membrane metallo endopeptidase (Mme), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu23141
Clone ID Related Accession (Same CDS sequence)	NM_008604.4 , XM_006501095.3 , NM_001357335.1 , NM_001289462.1 , NM_001289463.1 , XM_006501098.3
Accession Version	XM_006501095.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	neprilysin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ATGCTCCAAA GCCAAAGAAG 
AAACAGCGAT GGACTCCCCT GGAGATCAGC CTCTCTGTGC TTGTCTTGCT CCTGACTATC 
ATAGCGGTGA CAATGATCGC TCTCTATGCA ACCTATGATG ATGGCATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATT CAGAATATGG ATGCCTCTGT TGAGCCATGT 
ACAGACTTCT TCAAGTATGC TTGTGGAGGC TGGTTGAAAC GCAACGTCAT TCCCGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTTTA AGAGATGAGC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAAGACATA GTAGCAGTGC AGAAAGCAAA AACCTTGTAC 
AGATCGTGTA TAAATGAGTC TGCTATTGAT AGCAGAGGCG GACAACCTCT ACTCAAACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCAGATAACT GGGATCAAAC ATATGGCACT 
TCTTGGACAG CTGAGAAATC CATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GCACTGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGTTTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCTAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTGCAGAAC AACTTTTCCC TGGAGGTCAA TGGGAAGTCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TTGTTGTCTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTATGGG 
ACTACATCAG AAACTGCAAC ATGGAGACGG TGTGCTAACT ACGTCAATGG GAATATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCG GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGACTTGA TTGCACAAAT CCGGGAAGTT TTTATTCAGA CTTTGGATGA CCTTACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAAAAGGCCT TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT TTCCAATGAG AACAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAGGG AAGATGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAAGTG GACAAAGATG AGTGGATAAG TGGAGCAGCT 
GTAGTCAATG CATTTTATTC CTCAGGCCGA AATCAGATAG TCTTCCCCGC TGGCATTTTG 
CAGCCCCCAT TCTTCAGTGC TCAGCAATCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATTGGAC ATGAAATCAC ACATGGATTT GATGACAATG GAAGAAATTT CAATAAAGAT 
GGAGACCTCG TTGACTGGTG GACGCAGCAG TCGGCAAATA ATTTTAAAGA CCAATCCCAG 
TGTATGGTAT ACCAGTATGG AAACTTTTCC TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAATA CACTAGGAGA AAACATTGCT GATAATGGAG GTATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTTTT CCTGAACTTT GCCCAGGTGT GGTGTGGGAC CTACCGGCCA 
GAGTATGCAG TCAATTCTAT TAAAACAGAT GTACACAGTC CTGGAAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC CGAGTTTGCA GATGCCTTTC ATTGCCGCAA GAATTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501158.1
CDS	403..2655
Translation	MGRSESQMDITDINAPKPKKKQRWTPLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDASVEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLKLLPDIYGWPVASDNWDQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQSLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEVNGKSFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYREDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

Target ORF information:

RefSeq Version	XM_006501095.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus membrane metallo endopeptidase (Mme), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501095.3

ORF Insert Sequence:

ATGGGAAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ATGCTCCAAA GCCAAAGAAG 
AAACAGCGAT GGACTCCCCT GGAGATCAGC CTCTCTGTGC TTGTCTTGCT CCTGACTATC 
ATAGCGGTGA CAATGATCGC TCTCTATGCA ACCTATGATG ATGGCATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATT CAGAATATGG ATGCCTCTGT TGAGCCATGT 
ACAGACTTCT TCAAGTATGC TTGTGGAGGC TGGTTGAAAC GCAACGTCAT TCCCGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTTTA AGAGATGAGC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAAGACATA GTAGCAGTGC AGAAAGCAAA AACCTTGTAC 
AGATCGTGTA TAAATGAGTC TGCTATTGAT AGCAGAGGCG GACAACCTCT ACTCAAACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCAGATAACT GGGATCAAAC ATATGGCACT 
TCTTGGACAG CTGAGAAATC CATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GCACTGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGTTTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCTAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTGCAGAAC AACTTTTCCC TGGAGGTCAA TGGGAAGTCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TTGTTGTCTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTATGGG 
ACTACATCAG AAACTGCAAC ATGGAGACGG TGTGCTAACT ACGTCAATGG GAATATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCG GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGACTTGA TTGCACAAAT CCGGGAAGTT TTTATTCAGA CTTTGGATGA CCTTACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAAAAGGCCT TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT TTCCAATGAG AACAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAGGG AAGATGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAAGTG GACAAAGATG AGTGGATAAG TGGAGCAGCT 
GTAGTCAATG CATTTTATTC CTCAGGCCGA AATCAGATAG TCTTCCCCGC TGGCATTTTG 
CAGCCCCCAT TCTTCAGTGC TCAGCAATCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATTGGAC ATGAAATCAC ACATGGATTT GATGACAATG GAAGAAATTT CAATAAAGAT 
GGAGACCTCG TTGACTGGTG GACGCAGCAG TCGGCAAATA ATTTTAAAGA CCAATCCCAG 
TGTATGGTAT ACCAGTATGG AAACTTTTCC TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAATA CACTAGGAGA AAACATTGCT GATAATGGAG GTATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTTTT CCTGAACTTT GCCCAGGTGT GGTGTGGGAC CTACCGGCCA 
GAGTATGCAG TCAATTCTAT TAAAACAGAT GTACACAGTC CTGGAAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC CGAGTTTGCA GATGCCTTTC ATTGCCGCAA GAATTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mme ( XM_006501098.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501161.1 Mus musculus membrane metallo endopeptidase (Mme), transcript variant X1, mRNA.

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CloneID	OMu23141
Clone ID Related Accession (Same CDS sequence)	NM_008604.4 , XM_006501095.3 , NM_001357335.1 , NM_001289462.1 , NM_001289463.1 , XM_006501098.3
Accession Version	XM_006501098.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neprilysin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501098.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ATGCTCCAAA GCCAAAGAAG 
AAACAGCGAT GGACTCCCCT GGAGATCAGC CTCTCTGTGC TTGTCTTGCT CCTGACTATC 
ATAGCGGTGA CAATGATCGC TCTCTATGCA ACCTATGATG ATGGCATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATT CAGAATATGG ATGCCTCTGT TGAGCCATGT 
ACAGACTTCT TCAAGTATGC TTGTGGAGGC TGGTTGAAAC GCAACGTCAT TCCCGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTTTA AGAGATGAGC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAAGACATA GTAGCAGTGC AGAAAGCAAA AACCTTGTAC 
AGATCGTGTA TAAATGAGTC TGCTATTGAT AGCAGAGGCG GACAACCTCT ACTCAAACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCAGATAACT GGGATCAAAC ATATGGCACT 
TCTTGGACAG CTGAGAAATC CATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GCACTGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGTTTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCTAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTGCAGAAC AACTTTTCCC TGGAGGTCAA TGGGAAGTCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TTGTTGTCTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTATGGG 
ACTACATCAG AAACTGCAAC ATGGAGACGG TGTGCTAACT ACGTCAATGG GAATATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCG GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGACTTGA TTGCACAAAT CCGGGAAGTT TTTATTCAGA CTTTGGATGA CCTTACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAAAAGGCCT TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT TTCCAATGAG AACAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAGGG AAGATGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAAGTG GACAAAGATG AGTGGATAAG TGGAGCAGCT 
GTAGTCAATG CATTTTATTC CTCAGGCCGA AATCAGATAG TCTTCCCCGC TGGCATTTTG 
CAGCCCCCAT TCTTCAGTGC TCAGCAATCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATTGGAC ATGAAATCAC ACATGGATTT GATGACAATG GAAGAAATTT CAATAAAGAT 
GGAGACCTCG TTGACTGGTG GACGCAGCAG TCGGCAAATA ATTTTAAAGA CCAATCCCAG 
TGTATGGTAT ACCAGTATGG AAACTTTTCC TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAATA CACTAGGAGA AAACATTGCT GATAATGGAG GTATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTTTT CCTGAACTTT GCCCAGGTGT GGTGTGGGAC CTACCGGCCA 
GAGTATGCAG TCAATTCTAT TAAAACAGAT GTACACAGTC CTGGAAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC CGAGTTTGCA GATGCCTTTC ATTGCCGCAA GAATTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501161.1
CDS	117..2369
Translation	MGRSESQMDITDINAPKPKKKQRWTPLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDASVEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLKLLPDIYGWPVASDNWDQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQSLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEVNGKSFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYREDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

Target ORF information:

RefSeq Version	XM_006501098.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane metallo endopeptidase (Mme), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501098.3

ORF Insert Sequence:

ATGGGAAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ATGCTCCAAA GCCAAAGAAG 
AAACAGCGAT GGACTCCCCT GGAGATCAGC CTCTCTGTGC TTGTCTTGCT CCTGACTATC 
ATAGCGGTGA CAATGATCGC TCTCTATGCA ACCTATGATG ATGGCATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATT CAGAATATGG ATGCCTCTGT TGAGCCATGT 
ACAGACTTCT TCAAGTATGC TTGTGGAGGC TGGTTGAAAC GCAACGTCAT TCCCGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTTTA AGAGATGAGC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAAGACATA GTAGCAGTGC AGAAAGCAAA AACCTTGTAC 
AGATCGTGTA TAAATGAGTC TGCTATTGAT AGCAGAGGCG GACAACCTCT ACTCAAACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCAGATAACT GGGATCAAAC ATATGGCACT 
TCTTGGACAG CTGAGAAATC CATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GCACTGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGTTTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCTAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTGCAGAAC AACTTTTCCC TGGAGGTCAA TGGGAAGTCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TTGTTGTCTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTATGGG 
ACTACATCAG AAACTGCAAC ATGGAGACGG TGTGCTAACT ACGTCAATGG GAATATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCG GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGACTTGA TTGCACAAAT CCGGGAAGTT TTTATTCAGA CTTTGGATGA CCTTACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAAAAGGCCT TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT TTCCAATGAG AACAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAGGG AAGATGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAAGTG GACAAAGATG AGTGGATAAG TGGAGCAGCT 
GTAGTCAATG CATTTTATTC CTCAGGCCGA AATCAGATAG TCTTCCCCGC TGGCATTTTG 
CAGCCCCCAT TCTTCAGTGC TCAGCAATCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATTGGAC ATGAAATCAC ACATGGATTT GATGACAATG GAAGAAATTT CAATAAAGAT 
GGAGACCTCG TTGACTGGTG GACGCAGCAG TCGGCAAATA ATTTTAAAGA CCAATCCCAG 
TGTATGGTAT ACCAGTATGG AAACTTTTCC TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAATA CACTAGGAGA AAACATTGCT GATAATGGAG GTATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTTTT CCTGAACTTT GCCCAGGTGT GGTGTGGGAC CTACCGGCCA 
GAGTATGCAG TCAATTCTAT TAAAACAGAT GTACACAGTC CTGGAAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC CGAGTTTGCA GATGCCTTTC ATTGCCGCAA GAATTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biochemistry, Therapeutics, and Biomarker Implications of Neprilysin in Cardiorenal Disease.
Clinical chemistry63(1)108-115(2017 Jan)
Chen Y,Burnett JC Jr

A Test in Context: Neprilysin: Function, Inhibition, and Biomarker.
Journal of the American College of Cardiology68(6)639-53(2016 Aug)
Bayes-Genis A,Barallat J,Richards AM

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Mme cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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