Mme cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mme
Entrez Gene ID	24590
Full Name	membrane metallo-endopeptidase
Synonyms	CD10,Nep,SFE
General protein information	Preferred Names membrane metallo-endopeptidase Names neprilysin atriopeptidase enkephalinase membrane metallo-endopeptidase (neutral endopeptidase/enkephalinase) neutral endopeptidase 24.11 skin fibroblast elastase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q31
Summary	zinc-containing metallo-enzyme; involved in degradation of cardiac peptides [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017590629.1	XP_017446118.1	neprilysin isoform X1
XM_006232437.3	XP_006232499.1	neprilysin isoform X1
XM_017590630.1	XP_017446119.1	neprilysin isoform X1
NM_012608.2	NP_036740.1	neprilysin

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MME	NP_001179813.1
Homo sapiens (human)	MME	NP_009220.2
Pan troglodytes (chimpanzee)	MME	XP_001148532.1
Canis lupus familiaris (dog)	MME	XP_005634647.1
Gallus gallus (chicken)	MME	NP_001004412.1
	MME	XP_001105907.1
Mus musculus (house mouse)	Mme	NP_032630.2
Rattus norvegicus (Norway rat)	Mme	NP_036740.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of neprilysin in periodontitis-affected gingival tissues.
Nezu A, Kubota T, Maruyama S, Nagata M, Nohno K, Morozumi T, Yoshie H
Archives of oral biology7935-41(2017 Jul)

The Association of MME microRNA Binding Site Polymorphism with the Risk of Late Onset Alzheimer's Disease in Northern Han Chinese.
Liu CX, Tan L, Sun FR, Zhang W, Miao D, Tan MS, Wan Y, Tan CC, Yu JT, Tan L
Current neurovascular research14(2)90-95(2017)

Rare Variants in MME, Encoding Metalloprotease Neprilysin, Are Linked to Late-Onset Autosomal-Dominant Axonal Polyneuropathies.
Auer-Grumbach M, Toegel S, Schabh?ttl M, Weinmann D, Chiari C, Bennett DLH, Beetz C, Klein D, Andersen PM, B?hme I, Fink-Puches R, Gonzalez M, Harms MB, Motley W, Reilly MM, Renner W, Rudnik-Sch?neborn S, Schlotter-Weigel B, Themistocleous AC, Weishaupt JH, Ludolph AC, Wieland T, Tao F, Abreu L, Windhager R, Zitzelsberger M, Strom TM, Walther T, Scherer SS, Z?chner S, Martini R, Senderek J
American journal of human genetics99(3)607-623(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

hypoxia increased the expression of platelet-derived growth factor (PDGF) and transforming growth factor-beta1 (TGF-beta1) and decreased the expression of neprilysin (NEP), which contributed to the hypoxia-induced Endothelial-to-mesenchymal transition of pulmonary artery endothelial cells.
Title: The role of PDGF-B/TGF-?1/neprilysin network in regulating endothelial-to-mesenchymal transition in pulmonary artery remodeling.

The data obtained suggest that the increase of caspase-3 enzyme activity could affect NEP expression via proteolytic degradation of its transcription factor AICD
Title: [ROLE OF CASPASE-3 IN REGULATION OF THE CONTENT OF THE AMYLOID-DEGRADING NEUROPEPTIDASE NEPRILYSIN IN THE CORTEX OF RATS AFTER HYPOXIA].

the increased expression of NPR-C (r=-0.943 and -0.837 for mRNA and protein respectively, p<0.05) and NEP (r=-0.687 and -0.823 for mRNA and protein respectively, p<0.05) were accompanied by a signi fi cant decline in CNP.
Title: Paradoxical expressions of natriuretic peptide receptor-C and neutral endopeptidase account for C-type natriuretic peptide decline during the progression of experimental obstructive nephropathy.

Data suggest that the brain expression of neprilysin may promote amyloid beta-protein (Abeta)deposition in patients with Alzheimer's disease (AD).
Title: Leptin inhibits amyloid ?-protein degradation through decrease of neprilysin expression in primary cultured astrocytes.

These results suggested that ketamine suppresses the Abeta degradation of NEP by reducing p38 MAPK-mediated pathway activity.
Title: Ketamine reduces amyloid ?-protein degradation by suppressing neprilysin expression in primary cultured astrocytes.

The following Mme gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mme cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13276	NM_012608.2 Latest version!	Rattus norvegicus membrane metallo-endopeptidase (Mme), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa13276	XM_006232437.3 Latest version!	Rattus norvegicus membrane metallo-endopeptidase (Mme), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa13276	XM_017590630.1 Latest version!	Rattus norvegicus membrane metallo-endopeptidase (Mme), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa13276	XM_017590629.1 Latest version!	Rattus norvegicus membrane metallo-endopeptidase (Mme), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mme ( NM_012608.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036740.1 Rattus norvegicus membrane metallo-endopeptidase (Mme), mRNA.

Price & Availability↑

CloneID	ORa13276
Clone ID Related Accession (Same CDS sequence)	XM_017590629.1 , XM_006232437.3 , XM_017590630.1 , NM_012608.2
Accession Version	NM_012608.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	neprilysin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085753.1. On Nov 15, 2004 this sequence version replaced NM_012608.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085753.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGGGAAGAT CAGAAAGTCA GATGGATATT ACTGATATCA ATGCTCCAAA GCCGAAGAAG 
AAACAGCGAT GGACTCCACT GGAGATCAGC CTTTCTGTGC TCGTCTTGCT CCTGACTATC 
ATAGCTGTGA CAATGATTGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATC CAGAACATGG ATGCCTCTGC TGAGCCATGT 
ACGGACTTCT TCAAATATGC TTGTGGAGGC TGGTTGAAAC GCAATGTCAT CCCTGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTCTA AGAGATGAAC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAGGACATA GTAGCAGTGC AGAAAGCAAA AACTTTGTAC 
AGATCATGTA TAAATGAATC TGCTATTGAT AGCAGAGGTG GGCAACCTCT GCTCACACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCACAAAACT GGGAACAAAC ATATGGTACT 
TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GTACAGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGATTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCCAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTCCAAAAT AACTTCTCTC TGGAGATCAA TGGGAAGCCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TGGTTGTTTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTACGGG 
ACTACATCCG AAACTGCAAC CTGGAGACGG TGTGCCAACT ACGTCAATGG GAACATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCA GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGATTTGA TTGCACAAAT CCGTGAAGTT TTTATTCAGA CTTTAGATGA CCTCACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAGAAGGCCC TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT CTCCAATGAG AATAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAAGG AAGAGGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAGGTG GACAAAGATG AGTGGATAAG TGGCGCGGCG 
GTAGTCAATG CATTTTATTC CTCAGGCAGA AATCAGATCG TCTTCCCCGC CGGCATTTTG 
CAGCCCCCAT TCTTTAGTGC TCGGCAGTCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATCGGAC ATGAAATCAC ACATGGCTTT GATGACAATG GCAGAAATTT TAACAAAGAT 
GGAGACCTCG TTGACTGGTG GACTCAGCAG TCTGCAAATA ATTTCAAAGA CCAATCCCAG 
TGTATGGTGT ACCAGTATGG AAACTTTACA TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAACA CACTAGGAGA AAATATTGCT GATAATGGAG GGATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTCTT CTTGAACTTT GCCCAGGTGT GGTGTGGAAC CTACCGGCCA 
GAGTATGCAG TCAATTCCAT TAAAACAGAT GTACACAGTC CTGGCAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC TGAGTTTGCG GATGCCTTTC ATTGCCGCAA GAACTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036740.1
CDS	129..2381
Translation	MGRSESQMDITDINAPKPKKKQRWTPLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDASAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLTLLPDIYGWPVASQNWEQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQRLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEINGKPFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYKEEEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSARQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFTWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

Target ORF information:

RefSeq Version	NM_012608.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane metallo-endopeptidase (Mme), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012608.2

ORF Insert Sequence:

ATGGGAAGAT CAGAAAGTCA GATGGATATT ACTGATATCA ATGCTCCAAA GCCGAAGAAG 
AAACAGCGAT GGACTCCACT GGAGATCAGC CTTTCTGTGC TCGTCTTGCT CCTGACTATC 
ATAGCTGTGA CAATGATTGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATC CAGAACATGG ATGCCTCTGC TGAGCCATGT 
ACGGACTTCT TCAAATATGC TTGTGGAGGC TGGTTGAAAC GCAATGTCAT CCCTGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTCTA AGAGATGAAC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAGGACATA GTAGCAGTGC AGAAAGCAAA AACTTTGTAC 
AGATCATGTA TAAATGAATC TGCTATTGAT AGCAGAGGTG GGCAACCTCT GCTCACACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCACAAAACT GGGAACAAAC ATATGGTACT 
TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GTACAGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGATTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCCAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTCCAAAAT AACTTCTCTC TGGAGATCAA TGGGAAGCCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TGGTTGTTTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTACGGG 
ACTACATCCG AAACTGCAAC CTGGAGACGG TGTGCCAACT ACGTCAATGG GAACATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCA GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGATTTGA TTGCACAAAT CCGTGAAGTT TTTATTCAGA CTTTAGATGA CCTCACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAGAAGGCCC TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT CTCCAATGAG AATAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAAGG AAGAGGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAGGTG GACAAAGATG AGTGGATAAG TGGCGCGGCG 
GTAGTCAATG CATTTTATTC CTCAGGCAGA AATCAGATCG TCTTCCCCGC CGGCATTTTG 
CAGCCCCCAT TCTTTAGTGC TCGGCAGTCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATCGGAC ATGAAATCAC ACATGGCTTT GATGACAATG GCAGAAATTT TAACAAAGAT 
GGAGACCTCG TTGACTGGTG GACTCAGCAG TCTGCAAATA ATTTCAAAGA CCAATCCCAG 
TGTATGGTGT ACCAGTATGG AAACTTTACA TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAACA CACTAGGAGA AAATATTGCT GATAATGGAG GGATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTCTT CTTGAACTTT GCCCAGGTGT GGTGTGGAAC CTACCGGCCA 
GAGTATGCAG TCAATTCCAT TAAAACAGAT GTACACAGTC CTGGCAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC TGAGTTTGCG GATGCCTTTC ATTGCCGCAA GAACTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mme ( XM_017590629.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446118.1 Rattus norvegicus membrane metallo-endopeptidase (Mme), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa13276
Clone ID Related Accession (Same CDS sequence)	XM_017590629.1 , XM_006232437.3 , XM_017590630.1 , NM_012608.2
Accession Version	XM_017590629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neprilysin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAT CAGAAAGTCA GATGGATATT ACTGATATCA ATGCTCCAAA GCCGAAGAAG 
AAACAGCGAT GGACTCCACT GGAGATCAGC CTTTCTGTGC TCGTCTTGCT CCTGACTATC 
ATAGCTGTGA CAATGATTGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATC CAGAACATGG ATGCCTCTGC TGAGCCATGT 
ACGGACTTCT TCAAATATGC TTGTGGAGGC TGGTTGAAAC GCAATGTCAT CCCTGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTCTA AGAGATGAAC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAGGACATA GTAGCAGTGC AGAAAGCAAA AACTTTGTAC 
AGATCATGTA TAAATGAATC TGCTATTGAT AGCAGAGGTG GGCAACCTCT GCTCACACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCACAAAACT GGGAACAAAC ATATGGTACT 
TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GTACAGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGATTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCCAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTCCAAAAT AACTTCTCTC TGGAGATCAA TGGGAAGCCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TGGTTGTTTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTACGGG 
ACTACATCCG AAACTGCAAC CTGGAGACGG TGTGCCAACT ACGTCAATGG GAACATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCA GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGATTTGA TTGCACAAAT CCGTGAAGTT TTTATTCAGA CTTTAGATGA CCTCACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAGAAGGCCC TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT CTCCAATGAG AATAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAAGG AAGAGGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAGGTG GACAAAGATG AGTGGATAAG TGGCGCGGCG 
GTAGTCAATG CATTTTATTC CTCAGGCAGA AATCAGATCG TCTTCCCCGC CGGCATTTTG 
CAGCCCCCAT TCTTTAGTGC TCGGCAGTCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATCGGAC ATGAAATCAC ACATGGCTTT GATGACAATG GCAGAAATTT TAACAAAGAT 
GGAGACCTCG TTGACTGGTG GACTCAGCAG TCTGCAAATA ATTTCAAAGA CCAATCCCAG 
TGTATGGTGT ACCAGTATGG AAACTTTACA TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAACA CACTAGGAGA AAATATTGCT GATAATGGAG GGATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTCTT CTTGAACTTT GCCCAGGTGT GGTGTGGAAC CTACCGGCCA 
GAGTATGCAG TCAATTCCAT TAAAACAGAT GTACACAGTC CTGGCAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC TGAGTTTGCG GATGCCTTTC ATTGCCGCAA GAACTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446118.1
CDS	1893..4145
Translation	MGRSESQMDITDINAPKPKKKQRWTPLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDASAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLTLLPDIYGWPVASQNWEQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQRLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEINGKPFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYKEEEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSARQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFTWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

Target ORF information:

RefSeq Version	XM_017590629.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane metallo-endopeptidase (Mme), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590629.1

ORF Insert Sequence:

ATGGGAAGAT CAGAAAGTCA GATGGATATT ACTGATATCA ATGCTCCAAA GCCGAAGAAG 
AAACAGCGAT GGACTCCACT GGAGATCAGC CTTTCTGTGC TCGTCTTGCT CCTGACTATC 
ATAGCTGTGA CAATGATTGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATC CAGAACATGG ATGCCTCTGC TGAGCCATGT 
ACGGACTTCT TCAAATATGC TTGTGGAGGC TGGTTGAAAC GCAATGTCAT CCCTGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTCTA AGAGATGAAC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAGGACATA GTAGCAGTGC AGAAAGCAAA AACTTTGTAC 
AGATCATGTA TAAATGAATC TGCTATTGAT AGCAGAGGTG GGCAACCTCT GCTCACACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCACAAAACT GGGAACAAAC ATATGGTACT 
TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GTACAGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGATTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCCAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTCCAAAAT AACTTCTCTC TGGAGATCAA TGGGAAGCCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TGGTTGTTTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTACGGG 
ACTACATCCG AAACTGCAAC CTGGAGACGG TGTGCCAACT ACGTCAATGG GAACATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCA GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGATTTGA TTGCACAAAT CCGTGAAGTT TTTATTCAGA CTTTAGATGA CCTCACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAGAAGGCCC TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT CTCCAATGAG AATAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAAGG AAGAGGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAGGTG GACAAAGATG AGTGGATAAG TGGCGCGGCG 
GTAGTCAATG CATTTTATTC CTCAGGCAGA AATCAGATCG TCTTCCCCGC CGGCATTTTG 
CAGCCCCCAT TCTTTAGTGC TCGGCAGTCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATCGGAC ATGAAATCAC ACATGGCTTT GATGACAATG GCAGAAATTT TAACAAAGAT 
GGAGACCTCG TTGACTGGTG GACTCAGCAG TCTGCAAATA ATTTCAAAGA CCAATCCCAG 
TGTATGGTGT ACCAGTATGG AAACTTTACA TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAACA CACTAGGAGA AAATATTGCT GATAATGGAG GGATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTCTT CTTGAACTTT GCCCAGGTGT GGTGTGGAAC CTACCGGCCA 
GAGTATGCAG TCAATTCCAT TAAAACAGAT GTACACAGTC CTGGCAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC TGAGTTTGCG GATGCCTTTC ATTGCCGCAA GAACTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mme ( XM_006232437.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232499.1 Rattus norvegicus membrane metallo-endopeptidase (Mme), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa13276
Clone ID Related Accession (Same CDS sequence)	XM_017590629.1 , XM_006232437.3 , XM_017590630.1 , NM_012608.2
Accession Version	XM_006232437.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neprilysin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232437.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAT CAGAAAGTCA GATGGATATT ACTGATATCA ATGCTCCAAA GCCGAAGAAG 
AAACAGCGAT GGACTCCACT GGAGATCAGC CTTTCTGTGC TCGTCTTGCT CCTGACTATC 
ATAGCTGTGA CAATGATTGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATC CAGAACATGG ATGCCTCTGC TGAGCCATGT 
ACGGACTTCT TCAAATATGC TTGTGGAGGC TGGTTGAAAC GCAATGTCAT CCCTGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTCTA AGAGATGAAC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAGGACATA GTAGCAGTGC AGAAAGCAAA AACTTTGTAC 
AGATCATGTA TAAATGAATC TGCTATTGAT AGCAGAGGTG GGCAACCTCT GCTCACACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCACAAAACT GGGAACAAAC ATATGGTACT 
TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GTACAGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGATTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCCAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTCCAAAAT AACTTCTCTC TGGAGATCAA TGGGAAGCCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TGGTTGTTTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTACGGG 
ACTACATCCG AAACTGCAAC CTGGAGACGG TGTGCCAACT ACGTCAATGG GAACATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCA GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGATTTGA TTGCACAAAT CCGTGAAGTT TTTATTCAGA CTTTAGATGA CCTCACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAGAAGGCCC TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT CTCCAATGAG AATAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAAGG AAGAGGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAGGTG GACAAAGATG AGTGGATAAG TGGCGCGGCG 
GTAGTCAATG CATTTTATTC CTCAGGCAGA AATCAGATCG TCTTCCCCGC CGGCATTTTG 
CAGCCCCCAT TCTTTAGTGC TCGGCAGTCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATCGGAC ATGAAATCAC ACATGGCTTT GATGACAATG GCAGAAATTT TAACAAAGAT 
GGAGACCTCG TTGACTGGTG GACTCAGCAG TCTGCAAATA ATTTCAAAGA CCAATCCCAG 
TGTATGGTGT ACCAGTATGG AAACTTTACA TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAACA CACTAGGAGA AAATATTGCT GATAATGGAG GGATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTCTT CTTGAACTTT GCCCAGGTGT GGTGTGGAAC CTACCGGCCA 
GAGTATGCAG TCAATTCCAT TAAAACAGAT GTACACAGTC CTGGCAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC TGAGTTTGCG GATGCCTTTC ATTGCCGCAA GAACTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232499.1
CDS	162..2414
Translation	MGRSESQMDITDINAPKPKKKQRWTPLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDASAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLTLLPDIYGWPVASQNWEQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQRLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEINGKPFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYKEEEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSARQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFTWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

Target ORF information:

RefSeq Version	XM_006232437.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane metallo-endopeptidase (Mme), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232437.3

ORF Insert Sequence:

ATGGGAAGAT CAGAAAGTCA GATGGATATT ACTGATATCA ATGCTCCAAA GCCGAAGAAG 
AAACAGCGAT GGACTCCACT GGAGATCAGC CTTTCTGTGC TCGTCTTGCT CCTGACTATC 
ATAGCTGTGA CAATGATTGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATC CAGAACATGG ATGCCTCTGC TGAGCCATGT 
ACGGACTTCT TCAAATATGC TTGTGGAGGC TGGTTGAAAC GCAATGTCAT CCCTGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTCTA AGAGATGAAC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAGGACATA GTAGCAGTGC AGAAAGCAAA AACTTTGTAC 
AGATCATGTA TAAATGAATC TGCTATTGAT AGCAGAGGTG GGCAACCTCT GCTCACACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCACAAAACT GGGAACAAAC ATATGGTACT 
TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GTACAGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGATTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCCAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTCCAAAAT AACTTCTCTC TGGAGATCAA TGGGAAGCCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TGGTTGTTTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTACGGG 
ACTACATCCG AAACTGCAAC CTGGAGACGG TGTGCCAACT ACGTCAATGG GAACATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCA GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGATTTGA TTGCACAAAT CCGTGAAGTT TTTATTCAGA CTTTAGATGA CCTCACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAGAAGGCCC TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT CTCCAATGAG AATAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAAGG AAGAGGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAGGTG GACAAAGATG AGTGGATAAG TGGCGCGGCG 
GTAGTCAATG CATTTTATTC CTCAGGCAGA AATCAGATCG TCTTCCCCGC CGGCATTTTG 
CAGCCCCCAT TCTTTAGTGC TCGGCAGTCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATCGGAC ATGAAATCAC ACATGGCTTT GATGACAATG GCAGAAATTT TAACAAAGAT 
GGAGACCTCG TTGACTGGTG GACTCAGCAG TCTGCAAATA ATTTCAAAGA CCAATCCCAG 
TGTATGGTGT ACCAGTATGG AAACTTTACA TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAACA CACTAGGAGA AAATATTGCT GATAATGGAG GGATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTCTT CTTGAACTTT GCCCAGGTGT GGTGTGGAAC CTACCGGCCA 
GAGTATGCAG TCAATTCCAT TAAAACAGAT GTACACAGTC CTGGCAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC TGAGTTTGCG GATGCCTTTC ATTGCCGCAA GAACTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mme ( XM_017590630.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446119.1 Rattus norvegicus membrane metallo-endopeptidase (Mme), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa13276
Clone ID Related Accession (Same CDS sequence)	XM_017590629.1 , XM_006232437.3 , XM_017590630.1 , NM_012608.2
Accession Version	XM_017590630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neprilysin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAT CAGAAAGTCA GATGGATATT ACTGATATCA ATGCTCCAAA GCCGAAGAAG 
AAACAGCGAT GGACTCCACT GGAGATCAGC CTTTCTGTGC TCGTCTTGCT CCTGACTATC 
ATAGCTGTGA CAATGATTGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATC CAGAACATGG ATGCCTCTGC TGAGCCATGT 
ACGGACTTCT TCAAATATGC TTGTGGAGGC TGGTTGAAAC GCAATGTCAT CCCTGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTCTA AGAGATGAAC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAGGACATA GTAGCAGTGC AGAAAGCAAA AACTTTGTAC 
AGATCATGTA TAAATGAATC TGCTATTGAT AGCAGAGGTG GGCAACCTCT GCTCACACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCACAAAACT GGGAACAAAC ATATGGTACT 
TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GTACAGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGATTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCCAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTCCAAAAT AACTTCTCTC TGGAGATCAA TGGGAAGCCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TGGTTGTTTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTACGGG 
ACTACATCCG AAACTGCAAC CTGGAGACGG TGTGCCAACT ACGTCAATGG GAACATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCA GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGATTTGA TTGCACAAAT CCGTGAAGTT TTTATTCAGA CTTTAGATGA CCTCACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAGAAGGCCC TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT CTCCAATGAG AATAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAAGG AAGAGGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAGGTG GACAAAGATG AGTGGATAAG TGGCGCGGCG 
GTAGTCAATG CATTTTATTC CTCAGGCAGA AATCAGATCG TCTTCCCCGC CGGCATTTTG 
CAGCCCCCAT TCTTTAGTGC TCGGCAGTCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATCGGAC ATGAAATCAC ACATGGCTTT GATGACAATG GCAGAAATTT TAACAAAGAT 
GGAGACCTCG TTGACTGGTG GACTCAGCAG TCTGCAAATA ATTTCAAAGA CCAATCCCAG 
TGTATGGTGT ACCAGTATGG AAACTTTACA TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAACA CACTAGGAGA AAATATTGCT GATAATGGAG GGATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTCTT CTTGAACTTT GCCCAGGTGT GGTGTGGAAC CTACCGGCCA 
GAGTATGCAG TCAATTCCAT TAAAACAGAT GTACACAGTC CTGGCAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC TGAGTTTGCG GATGCCTTTC ATTGCCGCAA GAACTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446119.1
CDS	67..2319
Translation	MGRSESQMDITDINAPKPKKKQRWTPLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDASAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLTLLPDIYGWPVASQNWEQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQRLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEINGKPFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYKEEEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSARQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFTWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

Target ORF information:

RefSeq Version	XM_017590630.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane metallo-endopeptidase (Mme), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590630.1

ORF Insert Sequence:

ATGGGAAGAT CAGAAAGTCA GATGGATATT ACTGATATCA ATGCTCCAAA GCCGAAGAAG 
AAACAGCGAT GGACTCCACT GGAGATCAGC CTTTCTGTGC TCGTCTTGCT CCTGACTATC 
ATAGCTGTGA CAATGATTGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAATCATCA 
GACTGCATAA AATCAGCTGC TCGACTGATC CAGAACATGG ATGCCTCTGC TGAGCCATGT 
ACGGACTTCT TCAAATATGC TTGTGGAGGC TGGTTGAAAC GCAATGTCAT CCCTGAGACC 
AGTTCCCGAT ACAGTAATTT TGACATTCTA AGAGATGAAC TAGAAGTCAT TTTGAAAGAT 
GTCCTTCAAG AACCCAAAAC TGAGGACATA GTAGCAGTGC AGAAAGCAAA AACTTTGTAC 
AGATCATGTA TAAATGAATC TGCTATTGAT AGCAGAGGTG GGCAACCTCT GCTCACACTG 
TTACCAGATA TATATGGGTG GCCAGTAGCA TCACAAAACT GGGAACAAAC ATATGGTACT 
TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 
ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 
CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GTACAGGAAT ATATAAAGAG 
GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 
AGATTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 
AAAGAAATTG CCAATGCCAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 
AACAAAATGA CATTGGCCAA GCTCCAAAAT AACTTCTCTC TGGAGATCAA TGGGAAGCCA 
TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 
GAGGAAGAAG TGGTTGTTTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 
AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 
AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTACGGG 
ACTACATCCG AAACTGCAAC CTGGAGACGG TGTGCCAACT ACGTCAATGG GAACATGGAG 
AATGCTGTGG GGAGGCTTTA TGTGGAAGCA GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 
GAAGATTTGA TTGCACAAAT CCGTGAAGTT TTTATTCAGA CTTTAGATGA CCTCACTTGG 
ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAGAAGGCCC TGGCAATTAA AGAAAGGATT 
GGCTATCCTG ATGACATCAT CTCCAATGAG AATAAACTGA ATAATGAGTA TCTTGAGTTG 
AACTACAAGG AAGAGGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 
AAGCAGCTAA AGAAGCTCCG AGAAAAGGTG GACAAAGATG AGTGGATAAG TGGCGCGGCG 
GTAGTCAATG CATTTTATTC CTCAGGCAGA AATCAGATCG TCTTCCCCGC CGGCATTTTG 
CAGCCCCCAT TCTTTAGTGC TCGGCAGTCC AACTCATTGA ACTATGGGGG CATCGGCATG 
GTCATCGGAC ATGAAATCAC ACATGGCTTT GATGACAATG GCAGAAATTT TAACAAAGAT 
GGAGACCTCG TTGACTGGTG GACTCAGCAG TCTGCAAATA ATTTCAAAGA CCAATCCCAG 
TGTATGGTGT ACCAGTATGG AAACTTTACA TGGGACCTAG CAGGTGGACA GCATCTCAAT 
GGAATTAACA CACTAGGAGA AAATATTGCT GATAATGGAG GGATTGGCCA AGCATACAGA 
GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 
AATCACAAAC AACTATTCTT CTTGAACTTT GCCCAGGTGT GGTGTGGAAC CTACCGGCCA 
GAGTATGCAG TCAATTCCAT TAAAACAGAT GTACACAGTC CTGGCAATTT CAGGATCATT 
GGGACTTTGC AGAACTCTGC TGAGTTTGCG GATGCCTTTC ATTGCCGCAA GAACTCATAC 
ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of neprilysin in periodontitis-affected gingival tissues.
Archives of oral biology7935-41(2017 Jul)
Nezu A,Kubota T,Maruyama S,Nagata M,Nohno K,Morozumi T,Yoshie H

The Association of MME microRNA Binding Site Polymorphism with the Risk of Late Onset Alzheimer's Disease in Northern Han Chinese.
Current neurovascular research14(2)90-95(2017)
Liu CX,Tan L,Sun FR,Zhang W,Miao D,Tan MS,Wan Y,Tan CC,Yu JT,Tan L

Rare Variants in MME, Encoding Metalloprotease Neprilysin, Are Linked to Late-Onset Autosomal-Dominant Axonal Polyneuropathies.
American journal of human genetics99(3)607-623(2016 Sep)
Auer-Grumbach M,Toegel S,Schabh?ttl M,Weinmann D,Chiari C,Bennett DLH,Beetz C,Klein D,Andersen PM,B?hme I,Fink-Puches R,Gonzalez M,Harms MB,Motley W,Reilly MM,Renner W,Rudnik-Sch?neborn S,Schlotter-Weigel B,Themistocleous AC,Weishaupt JH,Ludolph AC,Wieland T,Tao F,Abreu L,Windhager R,Zitzelsberger M,Strom TM,Walther T,Scherer SS,Z?chner S,Martini R,Senderek J

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Mme cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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