MME cDNA ORF clone, Bos taurus(cattle)

The following MME gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MME cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa39701 NM_001192884.1
Latest version!
Bos taurus membrane metalloendopeptidase (MME), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa39701 XM_024990588.1
Latest version!
Bos taurus membrane metalloendopeptidase (MME), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa39701 XM_024990577.1
Latest version!
Bos taurus membrane metalloendopeptidase (MME), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa39701 XM_024990584.1
Latest version!
Bos taurus membrane metalloendopeptidase (MME), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa218841 XM_024990592.1
Latest version!
Bos taurus membrane metalloendopeptidase (MME), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa87801 XM_015473028.1
Latest version!
Bos taurus membrane metallo-endopeptidase (MME), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa87801 XM_015473030.1
Latest version!
Bos taurus membrane metallo-endopeptidase (MME), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa87801 XM_015473017.1
Latest version!
Bos taurus membrane metallo-endopeptidase (MME), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa87801 XM_015473025.1
Latest version!
Bos taurus membrane metallo-endopeptidase (MME), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa87801 XM_015473014.1
Latest version!
Bos taurus membrane metallo-endopeptidase (MME), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa87801 XM_015473018.1
Latest version!
Bos taurus membrane metallo-endopeptidase (MME), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa87801 XM_015473015.1
Latest version!
Bos taurus membrane metallo-endopeptidase (MME), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa39701
    Clone ID Related Accession (Same CDS sequence) XM_024990577.1 , NM_001192884.1 , XM_024990588.1 , XM_024990584.1
    Accession Version NM_001192884.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product neprilysin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000001.1. On Jul 17, 2010 this sequence version replaced XM_616885.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC151642.1, SRR6764281.32928.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATGCTAC AACTAAATCT GAAGATCGAA ATGATCCAAT GCTTCTTTAT
    AACAAAATGA CATTAGCTGA GGTCCAAAAT AAATTTTCAC TGGAATTCAG TGGGAAGCCA
    TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACCG TGAATATCAA TATTCCACAT
    GAGGAAGAAG TGATTGTTTA TGCTCCAGAA TATTTAACCA ATCTTAAGCT CATTCTTGCC
    AAATATTCTG CCAGAGATCT TCAAAATTTA ATGTCCTGGC GGTTCATAAT GGATCTTGTA
    AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC TCTTTATGGC
    ACAACATCAG AAACAGCAAC TTGGAGGCGC TGCGCAAACT ATGTCAATGG GAATATGGAA
    AATGCCGTGG GAAGGCTCTA TGTGGAAGCG GCATTTCCTG GAGATAGTAA ACATGTGGTT
    GAGGATTTAA TTGCCCAGAT CCGGGCGGTT TTTATTCAAA CTTTAGATGA CCTAACTTGG
    ATGGATGCTG AAACAAAAAA GAAAGCTGAA GAAAAGGCCT TAGCAATTAA AGAAAGAATT
    GGCTATCCTG ATGACATTAT TTCAAATGAT GACAAATTGA ATAAAGAGTA CCTTGAGTTG
    AGCTACCAGG AAGAAGAATA CTTTGAGAAT ATAATTCAAA ACTTGAAGTT TGGCCAAAAT
    AGACAACTAA AGAAGCTTCG AGAAAAGGTG GACAAGGATG AGTGGATAAG CGGAGCAGCT
    GTAGTCAATG CATTTTACTC GTCAGGCAGA AATCAGATAG TCTTCCCAGC TGGCATTCTG
    CAGCCACCCT TTTTCAGTGC CCAGCAGCCT AAGTCATTGA ACTATGGGGG CATTGGCATG
    GTCATAGGAC ACGAAATCAC CCATGGCTTC GATGACAATG GCAGAAATTT TAACAAGGAT
    GGAGACCTTG TTGACTGGTG GACTCAACAG TCTGCAAATA ATTTTAAAGA CCTATCCCAG
    TGCATGGTGT ACCAGTATGG GAACTTCTCC TGGGACCTAG CAAATGGACA GCATCTCAAT
    GGAATTAATA CACTGGGAGA AAACATTGCT GATAATGGTG GTATTGGCCA AGCATACAGA
    GCCTATCAAA ACTATGTTAA AAAGCATGGT GAAGAAAAAT TACTTCCTGG ACTTGACCTA
    AATCACAGAC AACTGTTCTT CCTGAATTTT GCCCAGGTGT GGTGTGGAAC CTACAGGACG
    GAGTATGCTC TCAACTCCAT TAAAACAGAT GTGCACAGTC CAGGCAATTT CAGGATTATT
    GGGACTTTGC AGAACTCTCC TCAGTTTTCA GAAGCCTTCC ATTGTCGCAA GAATTCATAC
    ATGAATCCAG AAAAGAAATG CCGGGTTTGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179813.1
    CDS346..2598
    Translation

    Target ORF information:

    RefSeq Version NM_001192884.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metalloendopeptidase (MME), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATGCTAC AACTAAATCT GAAGATCGAA ATGATCCAAT GCTTCTTTAT
    AACAAAATGA CATTAGCTGA GGTCCAAAAT AAATTTTCAC TGGAATTCAG TGGGAAGCCA
    TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACCG TGAATATCAA TATTCCACAT
    GAGGAAGAAG TGATTGTTTA TGCTCCAGAA TATTTAACCA ATCTTAAGCT CATTCTTGCC
    AAATATTCTG CCAGAGATCT TCAAAATTTA ATGTCCTGGC GGTTCATAAT GGATCTTGTA
    AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC TCTTTATGGC
    ACAACATCAG AAACAGCAAC TTGGAGGCGC TGCGCAAACT ATGTCAATGG GAATATGGAA
    AATGCCGTGG GAAGGCTCTA TGTGGAAGCG GCATTTCCTG GAGATAGTAA ACATGTGGTT
    GAGGATTTAA TTGCCCAGAT CCGGGCGGTT TTTATTCAAA CTTTAGATGA CCTAACTTGG
    ATGGATGCTG AAACAAAAAA GAAAGCTGAA GAAAAGGCCT TAGCAATTAA AGAAAGAATT
    GGCTATCCTG ATGACATTAT TTCAAATGAT GACAAATTGA ATAAAGAGTA CCTTGAGTTG
    AGCTACCAGG AAGAAGAATA CTTTGAGAAT ATAATTCAAA ACTTGAAGTT TGGCCAAAAT
    AGACAACTAA AGAAGCTTCG AGAAAAGGTG GACAAGGATG AGTGGATAAG CGGAGCAGCT
    GTAGTCAATG CATTTTACTC GTCAGGCAGA AATCAGATAG TCTTCCCAGC TGGCATTCTG
    CAGCCACCCT TTTTCAGTGC CCAGCAGCCT AAGTCATTGA ACTATGGGGG CATTGGCATG
    GTCATAGGAC ACGAAATCAC CCATGGCTTC GATGACAATG GCAGAAATTT TAACAAGGAT
    GGAGACCTTG TTGACTGGTG GACTCAACAG TCTGCAAATA ATTTTAAAGA CCTATCCCAG
    TGCATGGTGT ACCAGTATGG GAACTTCTCC TGGGACCTAG CAAATGGACA GCATCTCAAT
    GGAATTAATA CACTGGGAGA AAACATTGCT GATAATGGTG GTATTGGCCA AGCATACAGA
    GCCTATCAAA ACTATGTTAA AAAGCATGGT GAAGAAAAAT TACTTCCTGG ACTTGACCTA
    AATCACAGAC AACTGTTCTT CCTGAATTTT GCCCAGGTGT GGTGTGGAAC CTACAGGACG
    GAGTATGCTC TCAACTCCAT TAAAACAGAT GTGCACAGTC CAGGCAATTT CAGGATTATT
    GGGACTTTGC AGAACTCTCC TCAGTTTTCA GAAGCCTTCC ATTGTCGCAA GAATTCATAC
    ATGAATCCAG AAAAGAAATG CCGGGTTTGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39701
    Clone ID Related Accession (Same CDS sequence) XM_024990577.1 , NM_001192884.1 , XM_024990588.1 , XM_024990584.1
    Accession Version XM_024990577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neprilysin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATGCTAC AACTAAATCT GAAGATCGAA ATGATCCAAT GCTTCTTTAT
    AACAAAATGA CATTAGCTGA GGTCCAAAAT AAATTTTCAC TGGAATTCAG TGGGAAGCCA
    TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACCG TGAATATCAA TATTCCACAT
    GAGGAAGAAG TGATTGTTTA TGCTCCAGAA TATTTAACCA ATCTTAAGCT CATTCTTGCC
    AAATATTCTG CCAGAGATCT TCAAAATTTA ATGTCCTGGC GGTTCATAAT GGATCTTGTA
    AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC TCTTTATGGC
    ACAACATCAG AAACAGCAAC TTGGAGGCGC TGCGCAAACT ATGTCAATGG GAATATGGAA
    AATGCCGTGG GAAGGCTCTA TGTGGAAGCG GCATTTCCTG GAGATAGTAA ACATGTGGTT
    GAGGATTTAA TTGCCCAGAT CCGGGCGGTT TTTATTCAAA CTTTAGATGA CCTAACTTGG
    ATGGATGCTG AAACAAAAAA GAAAGCTGAA GAAAAGGCCT TAGCAATTAA AGAAAGAATT
    GGCTATCCTG ATGACATTAT TTCAAATGAT GACAAATTGA ATAAAGAGTA CCTTGAGTTG
    AGCTACCAGG AAGAAGAATA CTTTGAGAAT ATAATTCAAA ACTTGAAGTT TGGCCAAAAT
    AGACAACTAA AGAAGCTTCG AGAAAAGGTG GACAAGGATG AGTGGATAAG CGGAGCAGCT
    GTAGTCAATG CATTTTACTC GTCAGGCAGA AATCAGATAG TCTTCCCAGC TGGCATTCTG
    CAGCCACCCT TTTTCAGTGC CCAGCAGCCT AAGTCATTGA ACTATGGGGG CATTGGCATG
    GTCATAGGAC ACGAAATCAC CCATGGCTTC GATGACAATG GCAGAAATTT TAACAAGGAT
    GGAGACCTTG TTGACTGGTG GACTCAACAG TCTGCAAATA ATTTTAAAGA CCTATCCCAG
    TGCATGGTGT ACCAGTATGG GAACTTCTCC TGGGACCTAG CAAATGGACA GCATCTCAAT
    GGAATTAATA CACTGGGAGA AAACATTGCT GATAATGGTG GTATTGGCCA AGCATACAGA
    GCCTATCAAA ACTATGTTAA AAAGCATGGT GAAGAAAAAT TACTTCCTGG ACTTGACCTA
    AATCACAGAC AACTGTTCTT CCTGAATTTT GCCCAGGTGT GGTGTGGAAC CTACAGGACG
    GAGTATGCTC TCAACTCCAT TAAAACAGAT GTGCACAGTC CAGGCAATTT CAGGATTATT
    GGGACTTTGC AGAACTCTCC TCAGTTTTCA GAAGCCTTCC ATTGTCGCAA GAATTCATAC
    ATGAATCCAG AAAAGAAATG CCGGGTTTGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846345.1
    CDS217..2469
    Translation

    Target ORF information:

    RefSeq Version XM_024990577.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metalloendopeptidase (MME), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATGCTAC AACTAAATCT GAAGATCGAA ATGATCCAAT GCTTCTTTAT
    AACAAAATGA CATTAGCTGA GGTCCAAAAT AAATTTTCAC TGGAATTCAG TGGGAAGCCA
    TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACCG TGAATATCAA TATTCCACAT
    GAGGAAGAAG TGATTGTTTA TGCTCCAGAA TATTTAACCA ATCTTAAGCT CATTCTTGCC
    AAATATTCTG CCAGAGATCT TCAAAATTTA ATGTCCTGGC GGTTCATAAT GGATCTTGTA
    AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC TCTTTATGGC
    ACAACATCAG AAACAGCAAC TTGGAGGCGC TGCGCAAACT ATGTCAATGG GAATATGGAA
    AATGCCGTGG GAAGGCTCTA TGTGGAAGCG GCATTTCCTG GAGATAGTAA ACATGTGGTT
    GAGGATTTAA TTGCCCAGAT CCGGGCGGTT TTTATTCAAA CTTTAGATGA CCTAACTTGG
    ATGGATGCTG AAACAAAAAA GAAAGCTGAA GAAAAGGCCT TAGCAATTAA AGAAAGAATT
    GGCTATCCTG ATGACATTAT TTCAAATGAT GACAAATTGA ATAAAGAGTA CCTTGAGTTG
    AGCTACCAGG AAGAAGAATA CTTTGAGAAT ATAATTCAAA ACTTGAAGTT TGGCCAAAAT
    AGACAACTAA AGAAGCTTCG AGAAAAGGTG GACAAGGATG AGTGGATAAG CGGAGCAGCT
    GTAGTCAATG CATTTTACTC GTCAGGCAGA AATCAGATAG TCTTCCCAGC TGGCATTCTG
    CAGCCACCCT TTTTCAGTGC CCAGCAGCCT AAGTCATTGA ACTATGGGGG CATTGGCATG
    GTCATAGGAC ACGAAATCAC CCATGGCTTC GATGACAATG GCAGAAATTT TAACAAGGAT
    GGAGACCTTG TTGACTGGTG GACTCAACAG TCTGCAAATA ATTTTAAAGA CCTATCCCAG
    TGCATGGTGT ACCAGTATGG GAACTTCTCC TGGGACCTAG CAAATGGACA GCATCTCAAT
    GGAATTAATA CACTGGGAGA AAACATTGCT GATAATGGTG GTATTGGCCA AGCATACAGA
    GCCTATCAAA ACTATGTTAA AAAGCATGGT GAAGAAAAAT TACTTCCTGG ACTTGACCTA
    AATCACAGAC AACTGTTCTT CCTGAATTTT GCCCAGGTGT GGTGTGGAAC CTACAGGACG
    GAGTATGCTC TCAACTCCAT TAAAACAGAT GTGCACAGTC CAGGCAATTT CAGGATTATT
    GGGACTTTGC AGAACTCTCC TCAGTTTTCA GAAGCCTTCC ATTGTCGCAA GAATTCATAC
    ATGAATCCAG AAAAGAAATG CCGGGTTTGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39701
    Clone ID Related Accession (Same CDS sequence) XM_024990577.1 , NM_001192884.1 , XM_024990588.1 , XM_024990584.1
    Accession Version XM_024990588.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neprilysin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATGCTAC AACTAAATCT GAAGATCGAA ATGATCCAAT GCTTCTTTAT
    AACAAAATGA CATTAGCTGA GGTCCAAAAT AAATTTTCAC TGGAATTCAG TGGGAAGCCA
    TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACCG TGAATATCAA TATTCCACAT
    GAGGAAGAAG TGATTGTTTA TGCTCCAGAA TATTTAACCA ATCTTAAGCT CATTCTTGCC
    AAATATTCTG CCAGAGATCT TCAAAATTTA ATGTCCTGGC GGTTCATAAT GGATCTTGTA
    AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC TCTTTATGGC
    ACAACATCAG AAACAGCAAC TTGGAGGCGC TGCGCAAACT ATGTCAATGG GAATATGGAA
    AATGCCGTGG GAAGGCTCTA TGTGGAAGCG GCATTTCCTG GAGATAGTAA ACATGTGGTT
    GAGGATTTAA TTGCCCAGAT CCGGGCGGTT TTTATTCAAA CTTTAGATGA CCTAACTTGG
    ATGGATGCTG AAACAAAAAA GAAAGCTGAA GAAAAGGCCT TAGCAATTAA AGAAAGAATT
    GGCTATCCTG ATGACATTAT TTCAAATGAT GACAAATTGA ATAAAGAGTA CCTTGAGTTG
    AGCTACCAGG AAGAAGAATA CTTTGAGAAT ATAATTCAAA ACTTGAAGTT TGGCCAAAAT
    AGACAACTAA AGAAGCTTCG AGAAAAGGTG GACAAGGATG AGTGGATAAG CGGAGCAGCT
    GTAGTCAATG CATTTTACTC GTCAGGCAGA AATCAGATAG TCTTCCCAGC TGGCATTCTG
    CAGCCACCCT TTTTCAGTGC CCAGCAGCCT AAGTCATTGA ACTATGGGGG CATTGGCATG
    GTCATAGGAC ACGAAATCAC CCATGGCTTC GATGACAATG GCAGAAATTT TAACAAGGAT
    GGAGACCTTG TTGACTGGTG GACTCAACAG TCTGCAAATA ATTTTAAAGA CCTATCCCAG
    TGCATGGTGT ACCAGTATGG GAACTTCTCC TGGGACCTAG CAAATGGACA GCATCTCAAT
    GGAATTAATA CACTGGGAGA AAACATTGCT GATAATGGTG GTATTGGCCA AGCATACAGA
    GCCTATCAAA ACTATGTTAA AAAGCATGGT GAAGAAAAAT TACTTCCTGG ACTTGACCTA
    AATCACAGAC AACTGTTCTT CCTGAATTTT GCCCAGGTGT GGTGTGGAAC CTACAGGACG
    GAGTATGCTC TCAACTCCAT TAAAACAGAT GTGCACAGTC CAGGCAATTT CAGGATTATT
    GGGACTTTGC AGAACTCTCC TCAGTTTTCA GAAGCCTTCC ATTGTCGCAA GAATTCATAC
    ATGAATCCAG AAAAGAAATG CCGGGTTTGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846356.1
    CDS79..2331
    Translation

    Target ORF information:

    RefSeq Version XM_024990588.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metalloendopeptidase (MME), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990588.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATGCTAC AACTAAATCT GAAGATCGAA ATGATCCAAT GCTTCTTTAT
    AACAAAATGA CATTAGCTGA GGTCCAAAAT AAATTTTCAC TGGAATTCAG TGGGAAGCCA
    TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACCG TGAATATCAA TATTCCACAT
    GAGGAAGAAG TGATTGTTTA TGCTCCAGAA TATTTAACCA ATCTTAAGCT CATTCTTGCC
    AAATATTCTG CCAGAGATCT TCAAAATTTA ATGTCCTGGC GGTTCATAAT GGATCTTGTA
    AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC TCTTTATGGC
    ACAACATCAG AAACAGCAAC TTGGAGGCGC TGCGCAAACT ATGTCAATGG GAATATGGAA
    AATGCCGTGG GAAGGCTCTA TGTGGAAGCG GCATTTCCTG GAGATAGTAA ACATGTGGTT
    GAGGATTTAA TTGCCCAGAT CCGGGCGGTT TTTATTCAAA CTTTAGATGA CCTAACTTGG
    ATGGATGCTG AAACAAAAAA GAAAGCTGAA GAAAAGGCCT TAGCAATTAA AGAAAGAATT
    GGCTATCCTG ATGACATTAT TTCAAATGAT GACAAATTGA ATAAAGAGTA CCTTGAGTTG
    AGCTACCAGG AAGAAGAATA CTTTGAGAAT ATAATTCAAA ACTTGAAGTT TGGCCAAAAT
    AGACAACTAA AGAAGCTTCG AGAAAAGGTG GACAAGGATG AGTGGATAAG CGGAGCAGCT
    GTAGTCAATG CATTTTACTC GTCAGGCAGA AATCAGATAG TCTTCCCAGC TGGCATTCTG
    CAGCCACCCT TTTTCAGTGC CCAGCAGCCT AAGTCATTGA ACTATGGGGG CATTGGCATG
    GTCATAGGAC ACGAAATCAC CCATGGCTTC GATGACAATG GCAGAAATTT TAACAAGGAT
    GGAGACCTTG TTGACTGGTG GACTCAACAG TCTGCAAATA ATTTTAAAGA CCTATCCCAG
    TGCATGGTGT ACCAGTATGG GAACTTCTCC TGGGACCTAG CAAATGGACA GCATCTCAAT
    GGAATTAATA CACTGGGAGA AAACATTGCT GATAATGGTG GTATTGGCCA AGCATACAGA
    GCCTATCAAA ACTATGTTAA AAAGCATGGT GAAGAAAAAT TACTTCCTGG ACTTGACCTA
    AATCACAGAC AACTGTTCTT CCTGAATTTT GCCCAGGTGT GGTGTGGAAC CTACAGGACG
    GAGTATGCTC TCAACTCCAT TAAAACAGAT GTGCACAGTC CAGGCAATTT CAGGATTATT
    GGGACTTTGC AGAACTCTCC TCAGTTTTCA GAAGCCTTCC ATTGTCGCAA GAATTCATAC
    ATGAATCCAG AAAAGAAATG CCGGGTTTGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39701
    Clone ID Related Accession (Same CDS sequence) XM_024990577.1 , NM_001192884.1 , XM_024990588.1 , XM_024990584.1
    Accession Version XM_024990584.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neprilysin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATGCTAC AACTAAATCT GAAGATCGAA ATGATCCAAT GCTTCTTTAT
    AACAAAATGA CATTAGCTGA GGTCCAAAAT AAATTTTCAC TGGAATTCAG TGGGAAGCCA
    TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACCG TGAATATCAA TATTCCACAT
    GAGGAAGAAG TGATTGTTTA TGCTCCAGAA TATTTAACCA ATCTTAAGCT CATTCTTGCC
    AAATATTCTG CCAGAGATCT TCAAAATTTA ATGTCCTGGC GGTTCATAAT GGATCTTGTA
    AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC TCTTTATGGC
    ACAACATCAG AAACAGCAAC TTGGAGGCGC TGCGCAAACT ATGTCAATGG GAATATGGAA
    AATGCCGTGG GAAGGCTCTA TGTGGAAGCG GCATTTCCTG GAGATAGTAA ACATGTGGTT
    GAGGATTTAA TTGCCCAGAT CCGGGCGGTT TTTATTCAAA CTTTAGATGA CCTAACTTGG
    ATGGATGCTG AAACAAAAAA GAAAGCTGAA GAAAAGGCCT TAGCAATTAA AGAAAGAATT
    GGCTATCCTG ATGACATTAT TTCAAATGAT GACAAATTGA ATAAAGAGTA CCTTGAGTTG
    AGCTACCAGG AAGAAGAATA CTTTGAGAAT ATAATTCAAA ACTTGAAGTT TGGCCAAAAT
    AGACAACTAA AGAAGCTTCG AGAAAAGGTG GACAAGGATG AGTGGATAAG CGGAGCAGCT
    GTAGTCAATG CATTTTACTC GTCAGGCAGA AATCAGATAG TCTTCCCAGC TGGCATTCTG
    CAGCCACCCT TTTTCAGTGC CCAGCAGCCT AAGTCATTGA ACTATGGGGG CATTGGCATG
    GTCATAGGAC ACGAAATCAC CCATGGCTTC GATGACAATG GCAGAAATTT TAACAAGGAT
    GGAGACCTTG TTGACTGGTG GACTCAACAG TCTGCAAATA ATTTTAAAGA CCTATCCCAG
    TGCATGGTGT ACCAGTATGG GAACTTCTCC TGGGACCTAG CAAATGGACA GCATCTCAAT
    GGAATTAATA CACTGGGAGA AAACATTGCT GATAATGGTG GTATTGGCCA AGCATACAGA
    GCCTATCAAA ACTATGTTAA AAAGCATGGT GAAGAAAAAT TACTTCCTGG ACTTGACCTA
    AATCACAGAC AACTGTTCTT CCTGAATTTT GCCCAGGTGT GGTGTGGAAC CTACAGGACG
    GAGTATGCTC TCAACTCCAT TAAAACAGAT GTGCACAGTC CAGGCAATTT CAGGATTATT
    GGGACTTTGC AGAACTCTCC TCAGTTTTCA GAAGCCTTCC ATTGTCGCAA GAATTCATAC
    ATGAATCCAG AAAAGAAATG CCGGGTTTGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846352.1
    CDS285..2537
    Translation

    Target ORF information:

    RefSeq Version XM_024990584.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metalloendopeptidase (MME), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990584.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATGCTAC AACTAAATCT GAAGATCGAA ATGATCCAAT GCTTCTTTAT
    AACAAAATGA CATTAGCTGA GGTCCAAAAT AAATTTTCAC TGGAATTCAG TGGGAAGCCA
    TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACCG TGAATATCAA TATTCCACAT
    GAGGAAGAAG TGATTGTTTA TGCTCCAGAA TATTTAACCA ATCTTAAGCT CATTCTTGCC
    AAATATTCTG CCAGAGATCT TCAAAATTTA ATGTCCTGGC GGTTCATAAT GGATCTTGTA
    AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC TCTTTATGGC
    ACAACATCAG AAACAGCAAC TTGGAGGCGC TGCGCAAACT ATGTCAATGG GAATATGGAA
    AATGCCGTGG GAAGGCTCTA TGTGGAAGCG GCATTTCCTG GAGATAGTAA ACATGTGGTT
    GAGGATTTAA TTGCCCAGAT CCGGGCGGTT TTTATTCAAA CTTTAGATGA CCTAACTTGG
    ATGGATGCTG AAACAAAAAA GAAAGCTGAA GAAAAGGCCT TAGCAATTAA AGAAAGAATT
    GGCTATCCTG ATGACATTAT TTCAAATGAT GACAAATTGA ATAAAGAGTA CCTTGAGTTG
    AGCTACCAGG AAGAAGAATA CTTTGAGAAT ATAATTCAAA ACTTGAAGTT TGGCCAAAAT
    AGACAACTAA AGAAGCTTCG AGAAAAGGTG GACAAGGATG AGTGGATAAG CGGAGCAGCT
    GTAGTCAATG CATTTTACTC GTCAGGCAGA AATCAGATAG TCTTCCCAGC TGGCATTCTG
    CAGCCACCCT TTTTCAGTGC CCAGCAGCCT AAGTCATTGA ACTATGGGGG CATTGGCATG
    GTCATAGGAC ACGAAATCAC CCATGGCTTC GATGACAATG GCAGAAATTT TAACAAGGAT
    GGAGACCTTG TTGACTGGTG GACTCAACAG TCTGCAAATA ATTTTAAAGA CCTATCCCAG
    TGCATGGTGT ACCAGTATGG GAACTTCTCC TGGGACCTAG CAAATGGACA GCATCTCAAT
    GGAATTAATA CACTGGGAGA AAACATTGCT GATAATGGTG GTATTGGCCA AGCATACAGA
    GCCTATCAAA ACTATGTTAA AAAGCATGGT GAAGAAAAAT TACTTCCTGG ACTTGACCTA
    AATCACAGAC AACTGTTCTT CCTGAATTTT GCCCAGGTGT GGTGTGGAAC CTACAGGACG
    GAGTATGCTC TCAACTCCAT TAAAACAGAT GTGCACAGTC CAGGCAATTT CAGGATTATT
    GGGACTTTGC AGAACTCTCC TCAGTTTTCA GAAGCCTTCC ATTGTCGCAA GAATTCATAC
    ATGAATCCAG AAAAGAAATG CCGGGTTTGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218841
    Clone ID Related Accession (Same CDS sequence) XM_024990592.1
    Accession Version XM_024990592.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neprilysin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGATGT CCTTCAGGAA CCCAAAACTG AAGATATAAT AGCAGTGCAG
    AAAGCAAAAA TGTTATATAG ATCTTGTATA AATGAAACTG CTATTGAGAG CAGAGGCGGG
    GAACCTCTAC TTAGACTGTT GCCAGATATA TATGATTGGC CAGTGGCGCT AGAAAACTGG
    GAGCAAAAAT ATGGTTCTTC TTGGACAGCT GAGAAATCTA TTGCACAACT GAATTCTAAA
    TATGGAAAAA AAGTCATTAT TAATTTTTTT GTTGGCACTG ATGATAAAAA CTCTACGAAT
    CATATAATTC ATATTGACCA ACCTCAACTT GGCCTGCCTT CCAGAGACTA CTATGAATGC
    ACTGCAGCAT ATAAAGAGGC TTGTACGGCA TATGTGGATT TTATGATTTC TGTGGCCAAA
    TTGATTCGTC AGGAAAGAGG ACTGCCCATA GATGAAAACC AGCTTTCTTT GGAAATGAAT
    AAAGTTATGG ATTTGGAAAA AGAAATTGCC AATGCTACAA CTAAATCTGA AGATCGAAAT
    GATCCAATGC TTCTTTATAA CAAAATGACA TTAGCTGAGG TCCAAAATAA ATTTTCACTG
    GAATTCAGTG GGAAGCCATT CAGCTGGTCA AATTTCACAA ATGAAATCAT GTCAACCGTG
    AATATCAATA TTCCACATGA GGAAGAAGTG ATTGTTTATG CTCCAGAATA TTTAACCAAT
    CTTAAGCTCA TTCTTGCCAA ATATTCTGCC AGAGATCTTC AAAATTTAAT GTCCTGGCGG
    TTCATAATGG ATCTTGTAAG CAGCCTCAGC CGAAACTACA AGGAGTCCAG AAATGCTTTC
    CGCAAGGCTC TTTATGGCAC AACATCAGAA ACAGCAACTT GGAGGCGCTG CGCAAACTAT
    GTCAATGGGA ATATGGAAAA TGCCGTGGGA AGGCTCTATG TGGAAGCGGC ATTTCCTGGA
    GATAGTAAAC ATGTGGTTGA GGATTTAATT GCCCAGATCC GGGCGGTTTT TATTCAAACT
    TTAGATGACC TAACTTGGAT GGATGCTGAA ACAAAAAAGA AAGCTGAAGA AAAGGCCTTA
    GCAATTAAAG AAAGAATTGG CTATCCTGAT GACATTATTT CAAATGATGA CAAATTGAAT
    AAAGAGTACC TTGAGTTGAG CTACCAGGAA GAAGAATACT TTGAGAATAT AATTCAAAAC
    TTGAAGTTTG GCCAAAATAG ACAACTAAAG AAGCTTCGAG AAAAGGTGGA CAAGGATGAG
    TGGATAAGCG GAGCAGCTGT AGTCAATGCA TTTTACTCGT CAGGCAGAAA TCAGATAGTC
    TTCCCAGCTG GCATTCTGCA GCCACCCTTT TTCAGTGCCC AGCAGCCTAA GTCATTGAAC
    TATGGGGGCA TTGGCATGGT CATAGGACAC GAAATCACCC ATGGCTTCGA TGACAATGGC
    AGAAATTTTA ACAAGGATGG AGACCTTGTT GACTGGTGGA CTCAACAGTC TGCAAATAAT
    TTTAAAGACC TATCCCAGTG CATGGTGTAC CAGTATGGGA ACTTCTCCTG GGACCTAGCA
    AATGGACAGC ATCTCAATGG AATTAATACA CTGGGAGAAA ACATTGCTGA TAATGGTGGT
    ATTGGCCAAG CATACAGAGC CTATCAAAAC TATGTTAAAA AGCATGGTGA AGAAAAATTA
    CTTCCTGGAC TTGACCTAAA TCACAGACAA CTGTTCTTCC TGAATTTTGC CCAGGTGTGG
    TGTGGAACCT ACAGGACGGA GTATGCTCTC AACTCCATTA AAACAGATGT GCACAGTCCA
    GGCAATTTCA GGATTATTGG GACTTTGCAG AACTCTCCTC AGTTTTCAGA AGCCTTCCAT
    TGTCGCAAGA ATTCATACAT GAATCCAGAA AAGAAATGCC GGGTTTGGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846360.1
    CDS225..2315
    Translation

    Target ORF information:

    RefSeq Version XM_024990592.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metalloendopeptidase (MME), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990592.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGATGT CCTTCAGGAA CCCAAAACTG AAGATATAAT AGCAGTGCAG
    AAAGCAAAAA TGTTATATAG ATCTTGTATA AATGAAACTG CTATTGAGAG CAGAGGCGGG
    GAACCTCTAC TTAGACTGTT GCCAGATATA TATGATTGGC CAGTGGCGCT AGAAAACTGG
    GAGCAAAAAT ATGGTTCTTC TTGGACAGCT GAGAAATCTA TTGCACAACT GAATTCTAAA
    TATGGAAAAA AAGTCATTAT TAATTTTTTT GTTGGCACTG ATGATAAAAA CTCTACGAAT
    CATATAATTC ATATTGACCA ACCTCAACTT GGCCTGCCTT CCAGAGACTA CTATGAATGC
    ACTGCAGCAT ATAAAGAGGC TTGTACGGCA TATGTGGATT TTATGATTTC TGTGGCCAAA
    TTGATTCGTC AGGAAAGAGG ACTGCCCATA GATGAAAACC AGCTTTCTTT GGAAATGAAT
    AAAGTTATGG ATTTGGAAAA AGAAATTGCC AATGCTACAA CTAAATCTGA AGATCGAAAT
    GATCCAATGC TTCTTTATAA CAAAATGACA TTAGCTGAGG TCCAAAATAA ATTTTCACTG
    GAATTCAGTG GGAAGCCATT CAGCTGGTCA AATTTCACAA ATGAAATCAT GTCAACCGTG
    AATATCAATA TTCCACATGA GGAAGAAGTG ATTGTTTATG CTCCAGAATA TTTAACCAAT
    CTTAAGCTCA TTCTTGCCAA ATATTCTGCC AGAGATCTTC AAAATTTAAT GTCCTGGCGG
    TTCATAATGG ATCTTGTAAG CAGCCTCAGC CGAAACTACA AGGAGTCCAG AAATGCTTTC
    CGCAAGGCTC TTTATGGCAC AACATCAGAA ACAGCAACTT GGAGGCGCTG CGCAAACTAT
    GTCAATGGGA ATATGGAAAA TGCCGTGGGA AGGCTCTATG TGGAAGCGGC ATTTCCTGGA
    GATAGTAAAC ATGTGGTTGA GGATTTAATT GCCCAGATCC GGGCGGTTTT TATTCAAACT
    TTAGATGACC TAACTTGGAT GGATGCTGAA ACAAAAAAGA AAGCTGAAGA AAAGGCCTTA
    GCAATTAAAG AAAGAATTGG CTATCCTGAT GACATTATTT CAAATGATGA CAAATTGAAT
    AAAGAGTACC TTGAGTTGAG CTACCAGGAA GAAGAATACT TTGAGAATAT AATTCAAAAC
    TTGAAGTTTG GCCAAAATAG ACAACTAAAG AAGCTTCGAG AAAAGGTGGA CAAGGATGAG
    TGGATAAGCG GAGCAGCTGT AGTCAATGCA TTTTACTCGT CAGGCAGAAA TCAGATAGTC
    TTCCCAGCTG GCATTCTGCA GCCACCCTTT TTCAGTGCCC AGCAGCCTAA GTCATTGAAC
    TATGGGGGCA TTGGCATGGT CATAGGACAC GAAATCACCC ATGGCTTCGA TGACAATGGC
    AGAAATTTTA ACAAGGATGG AGACCTTGTT GACTGGTGGA CTCAACAGTC TGCAAATAAT
    TTTAAAGACC TATCCCAGTG CATGGTGTAC CAGTATGGGA ACTTCTCCTG GGACCTAGCA
    AATGGACAGC ATCTCAATGG AATTAATACA CTGGGAGAAA ACATTGCTGA TAATGGTGGT
    ATTGGCCAAG CATACAGAGC CTATCAAAAC TATGTTAAAA AGCATGGTGA AGAAAAATTA
    CTTCCTGGAC TTGACCTAAA TCACAGACAA CTGTTCTTCC TGAATTTTGC CCAGGTGTGG
    TGTGGAACCT ACAGGACGGA GTATGCTCTC AACTCCATTA AAACAGATGT GCACAGTCCA
    GGCAATTTCA GGATTATTGG GACTTTGCAG AACTCTCCTC AGTTTTCAGA AGCCTTCCAT
    TGTCGCAAGA ATTCATACAT GAATCCAGAA AAGAAATGCC GGGTTTGGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87801
    Clone ID Related Accession (Same CDS sequence) XM_015473014.1 , XM_015473015.1 , XM_015473017.1 , XM_015473025.1 , XM_015473028.1 , XM_015473018.1 , XM_015473030.1
    Accession Version XM_015473014.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neprilysin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328500.1
    CDS400..2727
    Translation

    Target ORF information:

    RefSeq Version XM_015473014.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metallo-endopeptidase (MME), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473014.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87801
    Clone ID Related Accession (Same CDS sequence) XM_015473014.1 , XM_015473015.1 , XM_015473017.1 , XM_015473025.1 , XM_015473028.1 , XM_015473018.1 , XM_015473030.1
    Accession Version XM_015473015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neprilysin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328501.1
    CDS74..2401
    Translation

    Target ORF information:

    RefSeq Version XM_015473015.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metallo-endopeptidase (MME), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87801
    Clone ID Related Accession (Same CDS sequence) XM_015473014.1 , XM_015473015.1 , XM_015473017.1 , XM_015473025.1 , XM_015473028.1 , XM_015473018.1 , XM_015473030.1
    Accession Version XM_015473017.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neprilysin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328503.1
    CDS186..2513
    Translation

    Target ORF information:

    RefSeq Version XM_015473017.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metallo-endopeptidase (MME), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473017.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87801
    Clone ID Related Accession (Same CDS sequence) XM_015473014.1 , XM_015473015.1 , XM_015473017.1 , XM_015473025.1 , XM_015473028.1 , XM_015473018.1 , XM_015473030.1
    Accession Version XM_015473025.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neprilysin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328511.1
    CDS127..2454
    Translation

    Target ORF information:

    RefSeq Version XM_015473025.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metallo-endopeptidase (MME), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473025.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87801
    Clone ID Related Accession (Same CDS sequence) XM_015473014.1 , XM_015473015.1 , XM_015473017.1 , XM_015473025.1 , XM_015473028.1 , XM_015473018.1 , XM_015473030.1
    Accession Version XM_015473028.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neprilysin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328514.1
    CDS112..2439
    Translation

    Target ORF information:

    RefSeq Version XM_015473028.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metallo-endopeptidase (MME), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473028.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87801
    Clone ID Related Accession (Same CDS sequence) XM_015473014.1 , XM_015473015.1 , XM_015473017.1 , XM_015473025.1 , XM_015473028.1 , XM_015473018.1 , XM_015473030.1
    Accession Version XM_015473018.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neprilysin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328504.1
    CDS225..2552
    Translation

    Target ORF information:

    RefSeq Version XM_015473018.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metallo-endopeptidase (MME), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473018.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87801
    Clone ID Related Accession (Same CDS sequence) XM_015473014.1 , XM_015473015.1 , XM_015473017.1 , XM_015473025.1 , XM_015473028.1 , XM_015473018.1 , XM_015473030.1
    Accession Version XM_015473030.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neprilysin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328516.1
    CDS51..2378
    Translation

    Target ORF information:

    RefSeq Version XM_015473030.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane metallo-endopeptidase (MME), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473030.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA ACACACCAAA GCCAAAGAAG 
    AAACAGAGAT GGACCCCATT GGAGATCAGC CTCTCCGTCC TCGTCCTGCT GCTCACCATC
    ATTGCAGTGA CAATGATAGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAGTCATCA
    GAGTGCATAA AATCAGCCGC TCGACTGATC CAGAACATGG ATGCCACTGT TGAGCCTTGT
    ACAGACTTTT TCAAATATGC TTGCGGAGGC TGGTTGAAAC GTAACGTCAT TCCGGAAACC
    AGCTCTCGTT ACAGTAACTT TGACATTTTA AGAGATGAAT TAGAAGTCGT TTTGAAAGAT
    GTCCTTCAGG AACCCAAAAC TGAAGATATA ATAGCAGTGC AGAAAGCAAA AATGTTATAT
    AGATCTTGTA TAAATGAAAC TGCTATTGAG AGCAGAGGCG GGGAACCTCT ACTTAGACTG
    TTGCCAGATA TATATGATTG GCCAGTGGCG CTAGAAAACT GGGAGCAAAA ATATGGTTCT
    TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGAAA AAAAGTCATT
    ATTAATTTTT TTGTTGGCAC TGATGATAAA AACTCTACGA ATCATATAAT TCATATTGAC
    CAACCTCAAC TTGGCCTGCC TTCCAGAGAC TACTATGAAT GCACTGCAGC ATATAAAGAG
    GCTTGTACGG CATATGTGGA TTTTATGATT TCTGTGGCCA AATTGATTCG TCAGGAAAGA
    GGACTGCCCA TAGATGAAAA CCAGCTTTCT TTGGAAATGA ATAAAGTTAT GGATTTGGAA
    AAAGAAATTG CCAATTCAGT GAAAACAGTA ATACTGGATG ATGATGATAT TACTAAACTC
    GTTAGGCCAC ATGAGCGGAC AGTCACAGAA GCTACAACTA AATCTGAAGA TCGAAATGAT
    CCAATGCTTC TTTATAACAA AATGACATTA GCTGAGGTCC AAAATAAATT TTCACTGGAA
    TTCAGTGGGA AGCCATTCAG CTGGTCAAAT TTCACAAATG AAATCATGTC AACCGTGAAT
    ATCAATATTC CACATGAGGA AGAAGTGATT GTTTATGCTC CAGAATATTT AACCAATCTT
    AAGCTCATTC TTGCCAAATA TTCTGCCAGA GATCTTCAAA ATTTAATGTC CTGGCGGTTC
    ATAATGGATC TTGTAAGCAG CCTCAGCCGA AACTACAAGG AGTCCAGAAA TGCTTTCCGC
    AAGGCTCTTT ATGGCACAAC ATCAGAAACA GCAACTTGGA GGCGCTGCGC AAACTATGTC
    AATGGGAATA TGGAAAATGC CGTGGGAAGG CTCTATGTGG AAGCGGCATT TCCTGGAGAT
    AGTAAACATG TGGTTGAGGA TTTAATTGCC CAGATCCGGG CGGTTTTTAT TCAAACTTTA
    GATGACCTAA CTTGGATGGA TGCTGAAACA AAAAAGAAAG CTGAAGAAAA GGCCTTAGCA
    ATTAAAGAAA GAATTGGCTA TCCTGATGAC ATTATTTCAA ATGATGACAA ATTGAATAAA
    GAGTACCTTG AGTTGAGCTA CCAGGAAGAA GAATACTTTG AGAATATAAT TCAAAACTTG
    AAGTTTGGCC AAAATAGACA ACTAAAGAAG CTTCGAGAAA AGGTGGACAA GGATGAGTGG
    ATAAGCGGAG CAGCTGTAGT CAATGCATTT TACTCGTCAG GCAGAAATCA GATAGTCTTC
    CCAGCTGGCA TTCTGCAGCC ACCCTTTTTC AGTGCCCAGC AGCCTAAGTC ATTGAACTAT
    GGGGGCATTG GCATGGTCAT AGGACACGAA ATCACCCATG GCTTCGATGA CAATGGCAGA
    AATTTTAACA AGGATGGAGA CCTTGTTGAC TGGTGGACTC AACAGTCTGC AAATAATTTT
    AAAGACCTAT CCCAGTGCAT GGTGTACCAG TATGGGAACT TCTCCTGGGA CCTAGCAAAT
    GGACAGCATC TCAATGGAAT TAATACACTG GGAGAAAACA TTGCTGATAA TGGTGGTATT
    GGCCAAGCAT ACAGAGCCTA TCAAAACTAT GTTAAAAAGC ATGGTGAAGA AAAATTACTT
    CCTGGACTTG ACCTAAATCA CAGACAACTG TTCTTCCTGA ATTTTGCCCA GGTGTGGTGT
    GGAACCTACA GGACGGAGTA TGCTCTCAAC TCCATTAAAA CAGATGTGCA CAGTCCAGGC
    AATTTCAGGA TTATTGGGAC TTTGCAGAAC TCTCCTCAGT TTTCAGAAGC CTTCCATTGT
    CGCAAGAATT CATACATGAA TCCAGAAAAG AAATGCCGGG TTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.