MME cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MME
Entrez Gene ID	477120
Full Name	membrane metalloendopeptidase
Synonyms	CD10
General protein information	Preferred Names membrane metalloendopeptidase Names neprilysin neprilysin-410 short isoform
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022408385.1	XP_022264093.1	neprilysin isoform X1
XM_005634590.2	XP_005634647.1	neprilysin isoform X1
NM_001289066.1	NP_001275995.1	neprilysin
XM_005634591.2	XP_005634648.1	neprilysin isoform X1
XM_005634590.1	XP_005634647.1	neprilysin isoform X1
XM_005634591.3	XP_005634648.1	neprilysin isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MME	NP_001179813.1
Homo sapiens (human)	MME	NP_009220.2
Pan troglodytes (chimpanzee)	MME	XP_001148532.1
Canis lupus familiaris (dog)	MME	XP_005634647.1
Gallus gallus (chicken)	MME	NP_001004412.1
	MME	XP_001105907.1
Mus musculus (house mouse)	Mme	NP_032630.2
Rattus norvegicus (Norway rat)	Mme	NP_036740.1

Gene Ontology
Bibliography

Related articles in PubMed

Temporal changes in neutral endopeptidase/CD10 immunoexpression in the cyclic and early pregnant canine endometrium.
Payan-Carreira R, Santos C, Miranda S, Pereira RM, Santos D, Pires MA
Theriogenology82(6)815-26(2014 Oct)

Use of CD10 as a marker of canine mammary myoepithelial cells.
S?nchez-C?spedes R, Su?rez-Bonnet A, Mill?n Y, Guil-Luna S, Reymundo C, Herr?ez P, Espinosa de Los Monteros A, Martin de Las Mulas J
Veterinary journal (London, England : 1997)195(2)192-9(2013 Feb)

Cloning, sequencing and expression in the dog of the main amyloid precursor protein isoforms and some of the enzymes related with their processing.
Sarasa L, Gallego C, Monle?n I, Olvera A, Canudas J, Monta??s M, Pesini P, Sarasa M
Neuroscience171(4)1091-101(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study evaluated the expression of CD10 during the estrous cycle and early pregnancy in health dogs.
Title: Temporal changes in neutral endopeptidase/CD10 immunoexpression in the cyclic and early pregnant canine endometrium.

The findings indicate that the CD10 antigen is a sensitive (although not specific) marker of canine myoepithelial cells in normal, dysplastic and neoplastic mammary tissue.
Title: Use of CD10 as a marker of canine mammary myoepithelial cells.

The following MME gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MME cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe34243	NM_001289066.1 Latest version!	Canis lupus familiaris membrane metalloendopeptidase (MME), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe24333	XM_005634590.2 Latest version!	Canis lupus familiaris membrane metalloendopeptidase (MME), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe24333	XM_022408385.1 Latest version!	Canis lupus familiaris membrane metalloendopeptidase (MME), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe24333	XM_005634591.3 Latest version!	Canis lupus familiaris membrane metalloendopeptidase (MME), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OCe24333	XM_005634590.1	Canis lupus familiaris membrane metallo-endopeptidase (MME), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe24333	XM_005634591.2	Canis lupus familiaris membrane metallo-endopeptidase (MME), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MME ( NM_001289066.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001275995.1 Canis lupus familiaris membrane metalloendopeptidase (MME), mRNA.

Price & Availability↑

CloneID	OCe34243
Clone ID Related Accession (Same CDS sequence)	NM_001289066.1
Accession Version	NM_001289066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	neprilysin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from JF451103.1 and AAEX03013696.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: JF451103.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA GCACTCCAAG GCCAAAGAAA 
CAACGATGGA CCTCCCTGGA GATCAGTCTC TCCGTCCTTG TCCTGCTCCT TACCATCATC 
GCCGTGACAA TGATCGCACT CTATGCAACC TATGATGATG GTATTTGCAA GTCATCAGAC 
TGCATAAAAT CAGCTGCTCG ACTGATCCAG AACATGGATG CCACTGCTGA GCCCTGTACA 
GACTTTTTTA AATACGCCTG TGGAGGCTGG TTGAAACGCA ACGTCATTCC TGAGACTAGC 
TCCCGTTACA GTAACTTTGA CATTTTAAGA GATGAACTTG AAGTCGTTTT GAAAGATGTT 
CTTCAGGAAC CCAAAACTGA GGATATAGTA GCAGTGCAGA AAGCAAAAAC GTTGTACAGA 
TCTTGTATAA ATGAATCTGC TATTGATAGC AGAGGCGGAC AACCTCTGCT CAGTCTGTTA 
CCAGATATCT ATGACTGGCC AGTAGCAACG GATAATTGGG AGCAAACATA TGGTACTTCT 
TGGACAGCTG AGAAGTCTAT TGCACAACTG AATTCTAAAT ATGGGAAAAA AGTCATTCTT 
AATTTTTTTG TTGGCACGGA TGATAAGAAC TCTGTGAACC ATATAATTCA TATTGACCAG 
CCTCGACTTG GCCTCCCGTC CAGAGACTAC TATGTCTGCA CTGGAATATA TGAAGAGGCT 
TGCACAGCGT ATAAAGATTT TATGATTTCT GTGGCCAGAT TGATTCGTAA GGAGAAAGGG 
CTGCTCATCG ATGAAAACCA GCTTTCTTTG GAAATGAACA GAGTTATGGA ATTGGAAAAA 
GAAATTGCCA GTGCTACAAC TAAACCTGAA GATCGAAATG ATCCCATGCT TCTTTATAAC 
AAAATGACAT TAGCCCAGAT CCAAAATAAC TTTACACTAG AGATTGATGG GAAGCCATTC 
AGCTGGTCAA ATTTCACAAA TGAAATCATG TCAACTGTGA ATATTAATAT TCCAAATGAG 
GAAGAAGTGG TCGTTTATGC TCCAGAATAT TTAACCAAAC TTAAGCTCAT TCTTACCAAA 
TATTCCTCCA GAGATCTTCA AAATTTAATG TCCTGGCGGT TCATAATGGA TCTTGTAAGC 
AGCCTCAGCC GAAACTACAA GGAGTCCCGA AATGCTTTCC GCAAGGCCCT TTATGGCACA 
ACATCAGAAA CAGCAACATG GAGACGTTGT GCGAACTATG TCAATGGGAA TATGGAAAAT 
GCTGTGGGAA GGCTGTACGT GGAAGCAGCA TTTGCTGGAG AGAGTAAACA TGTGGTTGAG 
GATTTGATTA CACAGATCCG TGCAGTTTTT ATTCAGACTT TAGATGACCT CACTTGGATG 
GATGCTGAAA CGAAAAAGAA AGCTGAGGAA AAGGCCTTAG CAATTAAAGA AAGAATTGGC 
TATCCTGATG ACATCATTTC AAATGATAGT AAACTGGATA ATGAATACCT GGAGTTGAAC 
TACAGGGAAG ATGAATACTT TGAAAACATA ATTCAAAACT TGAAGTTCAG CCAAAATAAA 
CAGCTCAAGA AGCTCCGAGA AAAAGTGGAC AAGGATGAGT GGATAAGCGG AGCAGCTGTA 
GTCAATGCAT TTTATTCTTC AGGCAGAAAT CAGATAGTCT TCCCAGCTGG CATTCTGCAG 
CCCCCCTTCT TCAGTGCCCA GCAGTCTAAC TCCTTGAACT ACGGGGGTAT CGGCATGGTC 
ATAGGACACG AAATCACCCA TGGCTTCGAT GACAATGGCA GAAATTTTAA CAAGGATGGA 
GACCTCGTTG ACTGGTGGAC TCAACAGTCG GCAAATAATT TTAAAGACCA ATCCCAATGC 
ATGGTGTACC AGTATGGAAA CTTCTCCTGG GACCTAGCAG GTGGACAGCA TCTCAATGGG 
ATTAATACAC TGGGAGAAAA TATCGCTGAT AATGGAGGTA TTGGCCAAGC ATACAGAGCC 
TATCAAAATT ATGTTAAAAA GAACGGTGAA GAAAAACTAC TTCCTGGACT TGACCTAAAT 
CACAAACAGC TGTTCTTCCT CAACTTTGCC CAGGTGTGGT GTGGGACCTA CAGGCCAGAG 
TATGCCGTCA ACTCCATTAA AACAGATGTT CACAGTCCAG GGAATTTCAG GATTATTGGG 
ACTTTGCAGA ACTCCCCTGA GTTTTCAGAG GCCTTCCATT GCCGCAAGAA TTCATACATG 
AATCCAGAAA AGAAATGCCG AGTTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275995.1
CDS	1..2250
Translation	MGRSESQMDITDISTPRPKKQRWTSLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDATAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLSLLPDIYDWPVATDNWEQTYGTSWTAEKSIAQLNSKYGKKVILNFFVGTDDKNSVNHIIHIDQPRLGLPSRDYYVCTGIYEEACTAYKDFMISVARLIRKEKGLLIDENQLSLEMNRVMELEKEIASATTKPEDRNDPMLLYNKMTLAQIQNNFTLEIDGKPFSWSNFTNEIMSTVNINIPNEEEVVVYAPEYLTKLKLILTKYSSRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLITQIRAVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNDSKLDNEYLELNYREDEYFENIIQNLKFSQNKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSPEFSEAFHCRKNSYMNPEKKCRVW

Target ORF information:

RefSeq Version	NM_001289066.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris membrane metalloendopeptidase (MME), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289066.1

ORF Insert Sequence:

ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA GCACTCCAAG GCCAAAGAAA 
CAACGATGGA CCTCCCTGGA GATCAGTCTC TCCGTCCTTG TCCTGCTCCT TACCATCATC 
GCCGTGACAA TGATCGCACT CTATGCAACC TATGATGATG GTATTTGCAA GTCATCAGAC 
TGCATAAAAT CAGCTGCTCG ACTGATCCAG AACATGGATG CCACTGCTGA GCCCTGTACA 
GACTTTTTTA AATACGCCTG TGGAGGCTGG TTGAAACGCA ACGTCATTCC TGAGACTAGC 
TCCCGTTACA GTAACTTTGA CATTTTAAGA GATGAACTTG AAGTCGTTTT GAAAGATGTT 
CTTCAGGAAC CCAAAACTGA GGATATAGTA GCAGTGCAGA AAGCAAAAAC GTTGTACAGA 
TCTTGTATAA ATGAATCTGC TATTGATAGC AGAGGCGGAC AACCTCTGCT CAGTCTGTTA 
CCAGATATCT ATGACTGGCC AGTAGCAACG GATAATTGGG AGCAAACATA TGGTACTTCT 
TGGACAGCTG AGAAGTCTAT TGCACAACTG AATTCTAAAT ATGGGAAAAA AGTCATTCTT 
AATTTTTTTG TTGGCACGGA TGATAAGAAC TCTGTGAACC ATATAATTCA TATTGACCAG 
CCTCGACTTG GCCTCCCGTC CAGAGACTAC TATGTCTGCA CTGGAATATA TGAAGAGGCT 
TGCACAGCGT ATAAAGATTT TATGATTTCT GTGGCCAGAT TGATTCGTAA GGAGAAAGGG 
CTGCTCATCG ATGAAAACCA GCTTTCTTTG GAAATGAACA GAGTTATGGA ATTGGAAAAA 
GAAATTGCCA GTGCTACAAC TAAACCTGAA GATCGAAATG ATCCCATGCT TCTTTATAAC 
AAAATGACAT TAGCCCAGAT CCAAAATAAC TTTACACTAG AGATTGATGG GAAGCCATTC 
AGCTGGTCAA ATTTCACAAA TGAAATCATG TCAACTGTGA ATATTAATAT TCCAAATGAG 
GAAGAAGTGG TCGTTTATGC TCCAGAATAT TTAACCAAAC TTAAGCTCAT TCTTACCAAA 
TATTCCTCCA GAGATCTTCA AAATTTAATG TCCTGGCGGT TCATAATGGA TCTTGTAAGC 
AGCCTCAGCC GAAACTACAA GGAGTCCCGA AATGCTTTCC GCAAGGCCCT TTATGGCACA 
ACATCAGAAA CAGCAACATG GAGACGTTGT GCGAACTATG TCAATGGGAA TATGGAAAAT 
GCTGTGGGAA GGCTGTACGT GGAAGCAGCA TTTGCTGGAG AGAGTAAACA TGTGGTTGAG 
GATTTGATTA CACAGATCCG TGCAGTTTTT ATTCAGACTT TAGATGACCT CACTTGGATG 
GATGCTGAAA CGAAAAAGAA AGCTGAGGAA AAGGCCTTAG CAATTAAAGA AAGAATTGGC 
TATCCTGATG ACATCATTTC AAATGATAGT AAACTGGATA ATGAATACCT GGAGTTGAAC 
TACAGGGAAG ATGAATACTT TGAAAACATA ATTCAAAACT TGAAGTTCAG CCAAAATAAA 
CAGCTCAAGA AGCTCCGAGA AAAAGTGGAC AAGGATGAGT GGATAAGCGG AGCAGCTGTA 
GTCAATGCAT TTTATTCTTC AGGCAGAAAT CAGATAGTCT TCCCAGCTGG CATTCTGCAG 
CCCCCCTTCT TCAGTGCCCA GCAGTCTAAC TCCTTGAACT ACGGGGGTAT CGGCATGGTC 
ATAGGACACG AAATCACCCA TGGCTTCGAT GACAATGGCA GAAATTTTAA CAAGGATGGA 
GACCTCGTTG ACTGGTGGAC TCAACAGTCG GCAAATAATT TTAAAGACCA ATCCCAATGC 
ATGGTGTACC AGTATGGAAA CTTCTCCTGG GACCTAGCAG GTGGACAGCA TCTCAATGGG 
ATTAATACAC TGGGAGAAAA TATCGCTGAT AATGGAGGTA TTGGCCAAGC ATACAGAGCC 
TATCAAAATT ATGTTAAAAA GAACGGTGAA GAAAAACTAC TTCCTGGACT TGACCTAAAT 
CACAAACAGC TGTTCTTCCT CAACTTTGCC CAGGTGTGGT GTGGGACCTA CAGGCCAGAG 
TATGCCGTCA ACTCCATTAA AACAGATGTT CACAGTCCAG GGAATTTCAG GATTATTGGG 
ACTTTGCAGA ACTCCCCTGA GTTTTCAGAG GCCTTCCATT GCCGCAAGAA TTCATACATG 
AATCCAGAAA AGAAATGCCG AGTTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MME ( XM_022408385.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022264093.1 Canis lupus familiaris membrane metalloendopeptidase (MME), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe24333
Clone ID Related Accession (Same CDS sequence)	XM_022408385.1 , XM_005634590.2 , XM_005634591.2 , XM_005634590.1 , XM_005634591.3
Accession Version	XM_022408385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	neprilysin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA GCACTCCAAG GCCAAAGAAG 
CAACGATGGA CCTCCCTGGA GATCAGTCTC TCCGTCCTTG TCCTGCTCCT TACCATCATC 
GCCGTGACAA TGATCGCACT CTATGCAACC TATGATGATG GTATTTGCAA GTCATCAGAC 
TGCATAAAAT CAGCCGCTCG ACTGATCCAG AACATGGATG CCACGGCTGA GCCCTGTACA 
GACTTTTTTA AATACGCCTG TGGAGGCTGG TTGAAACGCA ACGTCATTCC TGAGACTAGC 
TCCCGTTACA GTAACTTTGA CATTTTAAGA GATGAACTTG AAGTCGTTTT GAAAGATGTT 
CTTCAGGAAC CCAAAACTGA GGATATAGTA GCAGTGCAGA AAGCAAAAAC GTTGTACAGA 
TCTTGTATAA ATGAATCTGC TATTGATAGC AGAGGCGGAC AACCTCTGCT CAGTCTGTTA 
CCAGATATCT ATGACTGGCC AGTAGCAACG GATAATTGGG AGCAAACATA TGGTACTTCT 
TGGACAGCTG AGAAGTCTAT TGCACAACTG AATTCTAAAT ATGGGAAAAA AGTCATTCTT 
AATTTTTTTG TTGGCACGGA TGATAAGAAC TCTGTGAACC ATATAATTCA TATTGACCAG 
CCTCGACTTG GCCTCCCGTC CAGAGACTAC TATGTCTGCA CTGGAATATA TGAAGAGGCT 
TGCACAGCGT ATAAAGATTT TATGATTTCT GTGGCCAGAT TGATTCGTAA GGAGAAAGGA 
CTGCTCATCG ATGAAAACCA GCTTTCTTTG GAAATGAACA GAGTTATGGA ATTGGAAAAA 
GAAATTGCCA GTGCTACAAC TAAACCTGAA GATCGAAATG ATCCCATGCT TCTTTATAAC 
AAAATGACAT TAGCCCAGAT CCAAAATAAC TTTACACTAG AGATTGATGG GAAGCCATTC 
AGCTGGTCAA ATTTCACAAA TGAAATCATG TCAACTGTGA ATATTAATAT TCCAAATGAG 
GAAGAAGTGG TCGTTTATGC TCCAGAATAT TTAACCAAAC TTAAGCTCAT TCTTACCAAA 
TATTCCTCCA GAGATCTTCA AAATTTAATG TCCTGGCGGT TCATAATGGA TCTTGTAAGC 
AGCCTCAGCC GAAACTACAA GGAGTCCCGA AATGCTTTCC GCAAGGCCCT TTATGGCACA 
ACATCAGAAA CAGCAACATG GAGACGTTGT GCGAACTATG TCAATGGGAA TATGGAAAAT 
GCTGTGGGAA GGCTGTACGT GGAAGCAGCA TTTGCTGGAG AGAGTAAACA TGTGGTTGAG 
GATTTGATTA CACAGATCCG TGCAGTTTTT ATTCAGACTT TAGATGACCT CACTTGGATG 
GATGCTGAAA CGAAAAAGAA AGCTGAGGAA AAGGCCTTAG CAATTAAAGA AAGAATTGGC 
TATCCTGATG ACATCATTTC AAATGATAGT AAACTGGATA ATGAATACCT GGAGTTGAAC 
TACAGGGAAG ATGAATACTT TGAAAACATA ATTCAAAACT TGAAGTTCAG CCAAAATAAA 
CAGCTCAAGA AGCTCCGAGA AAAAGTGGAC AAGGATGAGT GGATAAGCGG AGCAGCTGTA 
GTCAATGCAT TTTATTCTTC AGGCAGAAAT CAGATAGTCT TCCCAGCTGG CATTCTGCAG 
CCCCCCTTCT TCAGTGCCCA GCAGTCTAAC TCCTTGAACT ACGGGGGTAT CGGCATGGTC 
ATAGGACACG AAATCACCCA TGGCTTCGAT GACAATGGCA GAAATTTTAA CAAGGATGGA 
GACCTCGTTG ACTGGTGGAC TCAACAGTCG GCAAATAATT TTAAAGACCA ATCCCAATGC 
ATGGTGTACC AGTATGGAAA CTTCTCCTGG GACCTAGCAG GTGGACAGCA TCTCAATGGG 
ATTAATACAC TGGGAGAAAA TATCGCTGAT AATGGAGGTA TTGGCCAAGC ATACAGAGCC 
TATCAAAATT ATGTTAAAAA GAACGGTGAA GAAAAACTAC TTCCTGGACT TGACCTAAAT 
CACAAACAGC TGTTCTTCCT CAACTTTGCC CAGGTGTGGT GTGGGACCTA CAGGCCAGAG 
TATGCCGTCA ACTCCATTAA AACAGATGTT CACAGTCCAG GGAATTTCAG GATTATTGGG 
ACTTTGCAGA ACTCCCCTGA GTTTTCAGAG GCCTTCCATT GCCGCAAGAA TTCATACATG 
AATCCAGAAA AGAAATGCCG AGTTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264093.1
CDS	361..2610
Translation	MGRSESQMDITDISTPRPKKQRWTSLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDATAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLSLLPDIYDWPVATDNWEQTYGTSWTAEKSIAQLNSKYGKKVILNFFVGTDDKNSVNHIIHIDQPRLGLPSRDYYVCTGIYEEACTAYKDFMISVARLIRKEKGLLIDENQLSLEMNRVMELEKEIASATTKPEDRNDPMLLYNKMTLAQIQNNFTLEIDGKPFSWSNFTNEIMSTVNINIPNEEEVVVYAPEYLTKLKLILTKYSSRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLITQIRAVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNDSKLDNEYLELNYREDEYFENIIQNLKFSQNKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSPEFSEAFHCRKNSYMNPEKKCRVW

Target ORF information:

RefSeq Version	XM_022408385.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris membrane metalloendopeptidase (MME), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408385.1

ORF Insert Sequence:

ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA GCACTCCAAG GCCAAAGAAG 
CAACGATGGA CCTCCCTGGA GATCAGTCTC TCCGTCCTTG TCCTGCTCCT TACCATCATC 
GCCGTGACAA TGATCGCACT CTATGCAACC TATGATGATG GTATTTGCAA GTCATCAGAC 
TGCATAAAAT CAGCCGCTCG ACTGATCCAG AACATGGATG CCACGGCTGA GCCCTGTACA 
GACTTTTTTA AATACGCCTG TGGAGGCTGG TTGAAACGCA ACGTCATTCC TGAGACTAGC 
TCCCGTTACA GTAACTTTGA CATTTTAAGA GATGAACTTG AAGTCGTTTT GAAAGATGTT 
CTTCAGGAAC CCAAAACTGA GGATATAGTA GCAGTGCAGA AAGCAAAAAC GTTGTACAGA 
TCTTGTATAA ATGAATCTGC TATTGATAGC AGAGGCGGAC AACCTCTGCT CAGTCTGTTA 
CCAGATATCT ATGACTGGCC AGTAGCAACG GATAATTGGG AGCAAACATA TGGTACTTCT 
TGGACAGCTG AGAAGTCTAT TGCACAACTG AATTCTAAAT ATGGGAAAAA AGTCATTCTT 
AATTTTTTTG TTGGCACGGA TGATAAGAAC TCTGTGAACC ATATAATTCA TATTGACCAG 
CCTCGACTTG GCCTCCCGTC CAGAGACTAC TATGTCTGCA CTGGAATATA TGAAGAGGCT 
TGCACAGCGT ATAAAGATTT TATGATTTCT GTGGCCAGAT TGATTCGTAA GGAGAAAGGA 
CTGCTCATCG ATGAAAACCA GCTTTCTTTG GAAATGAACA GAGTTATGGA ATTGGAAAAA 
GAAATTGCCA GTGCTACAAC TAAACCTGAA GATCGAAATG ATCCCATGCT TCTTTATAAC 
AAAATGACAT TAGCCCAGAT CCAAAATAAC TTTACACTAG AGATTGATGG GAAGCCATTC 
AGCTGGTCAA ATTTCACAAA TGAAATCATG TCAACTGTGA ATATTAATAT TCCAAATGAG 
GAAGAAGTGG TCGTTTATGC TCCAGAATAT TTAACCAAAC TTAAGCTCAT TCTTACCAAA 
TATTCCTCCA GAGATCTTCA AAATTTAATG TCCTGGCGGT TCATAATGGA TCTTGTAAGC 
AGCCTCAGCC GAAACTACAA GGAGTCCCGA AATGCTTTCC GCAAGGCCCT TTATGGCACA 
ACATCAGAAA CAGCAACATG GAGACGTTGT GCGAACTATG TCAATGGGAA TATGGAAAAT 
GCTGTGGGAA GGCTGTACGT GGAAGCAGCA TTTGCTGGAG AGAGTAAACA TGTGGTTGAG 
GATTTGATTA CACAGATCCG TGCAGTTTTT ATTCAGACTT TAGATGACCT CACTTGGATG 
GATGCTGAAA CGAAAAAGAA AGCTGAGGAA AAGGCCTTAG CAATTAAAGA AAGAATTGGC 
TATCCTGATG ACATCATTTC AAATGATAGT AAACTGGATA ATGAATACCT GGAGTTGAAC 
TACAGGGAAG ATGAATACTT TGAAAACATA ATTCAAAACT TGAAGTTCAG CCAAAATAAA 
CAGCTCAAGA AGCTCCGAGA AAAAGTGGAC AAGGATGAGT GGATAAGCGG AGCAGCTGTA 
GTCAATGCAT TTTATTCTTC AGGCAGAAAT CAGATAGTCT TCCCAGCTGG CATTCTGCAG 
CCCCCCTTCT TCAGTGCCCA GCAGTCTAAC TCCTTGAACT ACGGGGGTAT CGGCATGGTC 
ATAGGACACG AAATCACCCA TGGCTTCGAT GACAATGGCA GAAATTTTAA CAAGGATGGA 
GACCTCGTTG ACTGGTGGAC TCAACAGTCG GCAAATAATT TTAAAGACCA ATCCCAATGC 
ATGGTGTACC AGTATGGAAA CTTCTCCTGG GACCTAGCAG GTGGACAGCA TCTCAATGGG 
ATTAATACAC TGGGAGAAAA TATCGCTGAT AATGGAGGTA TTGGCCAAGC ATACAGAGCC 
TATCAAAATT ATGTTAAAAA GAACGGTGAA GAAAAACTAC TTCCTGGACT TGACCTAAAT 
CACAAACAGC TGTTCTTCCT CAACTTTGCC CAGGTGTGGT GTGGGACCTA CAGGCCAGAG 
TATGCCGTCA ACTCCATTAA AACAGATGTT CACAGTCCAG GGAATTTCAG GATTATTGGG 
ACTTTGCAGA ACTCCCCTGA GTTTTCAGAG GCCTTCCATT GCCGCAAGAA TTCATACATG 
AATCCAGAAA AGAAATGCCG AGTTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MME ( XM_005634590.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634647.1 Canis lupus familiaris membrane metalloendopeptidase (MME), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe24333
Clone ID Related Accession (Same CDS sequence)	XM_022408385.1 , XM_005634590.2 , XM_005634591.2 , XM_005634590.1 , XM_005634591.3
Accession Version	XM_005634590.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	neprilysin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634590.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA GCACTCCAAG GCCAAAGAAG 
CAACGATGGA CCTCCCTGGA GATCAGTCTC TCCGTCCTTG TCCTGCTCCT TACCATCATC 
GCCGTGACAA TGATCGCACT CTATGCAACC TATGATGATG GTATTTGCAA GTCATCAGAC 
TGCATAAAAT CAGCCGCTCG ACTGATCCAG AACATGGATG CCACGGCTGA GCCCTGTACA 
GACTTTTTTA AATACGCCTG TGGAGGCTGG TTGAAACGCA ACGTCATTCC TGAGACTAGC 
TCCCGTTACA GTAACTTTGA CATTTTAAGA GATGAACTTG AAGTCGTTTT GAAAGATGTT 
CTTCAGGAAC CCAAAACTGA GGATATAGTA GCAGTGCAGA AAGCAAAAAC GTTGTACAGA 
TCTTGTATAA ATGAATCTGC TATTGATAGC AGAGGCGGAC AACCTCTGCT CAGTCTGTTA 
CCAGATATCT ATGACTGGCC AGTAGCAACG GATAATTGGG AGCAAACATA TGGTACTTCT 
TGGACAGCTG AGAAGTCTAT TGCACAACTG AATTCTAAAT ATGGGAAAAA AGTCATTCTT 
AATTTTTTTG TTGGCACGGA TGATAAGAAC TCTGTGAACC ATATAATTCA TATTGACCAG 
CCTCGACTTG GCCTCCCGTC CAGAGACTAC TATGTCTGCA CTGGAATATA TGAAGAGGCT 
TGCACAGCGT ATAAAGATTT TATGATTTCT GTGGCCAGAT TGATTCGTAA GGAGAAAGGA 
CTGCTCATCG ATGAAAACCA GCTTTCTTTG GAAATGAACA GAGTTATGGA ATTGGAAAAA 
GAAATTGCCA GTGCTACAAC TAAACCTGAA GATCGAAATG ATCCCATGCT TCTTTATAAC 
AAAATGACAT TAGCCCAGAT CCAAAATAAC TTTACACTAG AGATTGATGG GAAGCCATTC 
AGCTGGTCAA ATTTCACAAA TGAAATCATG TCAACTGTGA ATATTAATAT TCCAAATGAG 
GAAGAAGTGG TCGTTTATGC TCCAGAATAT TTAACCAAAC TTAAGCTCAT TCTTACCAAA 
TATTCCTCCA GAGATCTTCA AAATTTAATG TCCTGGCGGT TCATAATGGA TCTTGTAAGC 
AGCCTCAGCC GAAACTACAA GGAGTCCCGA AATGCTTTCC GCAAGGCCCT TTATGGCACA 
ACATCAGAAA CAGCAACATG GAGACGTTGT GCGAACTATG TCAATGGGAA TATGGAAAAT 
GCTGTGGGAA GGCTGTACGT GGAAGCAGCA TTTGCTGGAG AGAGTAAACA TGTGGTTGAG 
GATTTGATTA CACAGATCCG TGCAGTTTTT ATTCAGACTT TAGATGACCT CACTTGGATG 
GATGCTGAAA CGAAAAAGAA AGCTGAGGAA AAGGCCTTAG CAATTAAAGA AAGAATTGGC 
TATCCTGATG ACATCATTTC AAATGATAGT AAACTGGATA ATGAATACCT GGAGTTGAAC 
TACAGGGAAG ATGAATACTT TGAAAACATA ATTCAAAACT TGAAGTTCAG CCAAAATAAA 
CAGCTCAAGA AGCTCCGAGA AAAAGTGGAC AAGGATGAGT GGATAAGCGG AGCAGCTGTA 
GTCAATGCAT TTTATTCTTC AGGCAGAAAT CAGATAGTCT TCCCAGCTGG CATTCTGCAG 
CCCCCCTTCT TCAGTGCCCA GCAGTCTAAC TCCTTGAACT ACGGGGGTAT CGGCATGGTC 
ATAGGACACG AAATCACCCA TGGCTTCGAT GACAATGGCA GAAATTTTAA CAAGGATGGA 
GACCTCGTTG ACTGGTGGAC TCAACAGTCG GCAAATAATT TTAAAGACCA ATCCCAATGC 
ATGGTGTACC AGTATGGAAA CTTCTCCTGG GACCTAGCAG GTGGACAGCA TCTCAATGGG 
ATTAATACAC TGGGAGAAAA TATCGCTGAT AATGGAGGTA TTGGCCAAGC ATACAGAGCC 
TATCAAAATT ATGTTAAAAA GAACGGTGAA GAAAAACTAC TTCCTGGACT TGACCTAAAT 
CACAAACAGC TGTTCTTCCT CAACTTTGCC CAGGTGTGGT GTGGGACCTA CAGGCCAGAG 
TATGCCGTCA ACTCCATTAA AACAGATGTT CACAGTCCAG GGAATTTCAG GATTATTGGG 
ACTTTGCAGA ACTCCCCTGA GTTTTCAGAG GCCTTCCATT GCCGCAAGAA TTCATACATG 
AATCCAGAAA AGAAATGCCG AGTTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634647.1
CDS	118..2367
Translation	MGRSESQMDITDISTPRPKKQRWTSLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDATAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLSLLPDIYDWPVATDNWEQTYGTSWTAEKSIAQLNSKYGKKVILNFFVGTDDKNSVNHIIHIDQPRLGLPSRDYYVCTGIYEEACTAYKDFMISVARLIRKEKGLLIDENQLSLEMNRVMELEKEIASATTKPEDRNDPMLLYNKMTLAQIQNNFTLEIDGKPFSWSNFTNEIMSTVNINIPNEEEVVVYAPEYLTKLKLILTKYSSRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLITQIRAVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNDSKLDNEYLELNYREDEYFENIIQNLKFSQNKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSPEFSEAFHCRKNSYMNPEKKCRVW

Target ORF information:

RefSeq Version	XM_005634590.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris membrane metalloendopeptidase (MME), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634590.2

ORF Insert Sequence:

ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA GCACTCCAAG GCCAAAGAAG 
CAACGATGGA CCTCCCTGGA GATCAGTCTC TCCGTCCTTG TCCTGCTCCT TACCATCATC 
GCCGTGACAA TGATCGCACT CTATGCAACC TATGATGATG GTATTTGCAA GTCATCAGAC 
TGCATAAAAT CAGCCGCTCG ACTGATCCAG AACATGGATG CCACGGCTGA GCCCTGTACA 
GACTTTTTTA AATACGCCTG TGGAGGCTGG TTGAAACGCA ACGTCATTCC TGAGACTAGC 
TCCCGTTACA GTAACTTTGA CATTTTAAGA GATGAACTTG AAGTCGTTTT GAAAGATGTT 
CTTCAGGAAC CCAAAACTGA GGATATAGTA GCAGTGCAGA AAGCAAAAAC GTTGTACAGA 
TCTTGTATAA ATGAATCTGC TATTGATAGC AGAGGCGGAC AACCTCTGCT CAGTCTGTTA 
CCAGATATCT ATGACTGGCC AGTAGCAACG GATAATTGGG AGCAAACATA TGGTACTTCT 
TGGACAGCTG AGAAGTCTAT TGCACAACTG AATTCTAAAT ATGGGAAAAA AGTCATTCTT 
AATTTTTTTG TTGGCACGGA TGATAAGAAC TCTGTGAACC ATATAATTCA TATTGACCAG 
CCTCGACTTG GCCTCCCGTC CAGAGACTAC TATGTCTGCA CTGGAATATA TGAAGAGGCT 
TGCACAGCGT ATAAAGATTT TATGATTTCT GTGGCCAGAT TGATTCGTAA GGAGAAAGGA 
CTGCTCATCG ATGAAAACCA GCTTTCTTTG GAAATGAACA GAGTTATGGA ATTGGAAAAA 
GAAATTGCCA GTGCTACAAC TAAACCTGAA GATCGAAATG ATCCCATGCT TCTTTATAAC 
AAAATGACAT TAGCCCAGAT CCAAAATAAC TTTACACTAG AGATTGATGG GAAGCCATTC 
AGCTGGTCAA ATTTCACAAA TGAAATCATG TCAACTGTGA ATATTAATAT TCCAAATGAG 
GAAGAAGTGG TCGTTTATGC TCCAGAATAT TTAACCAAAC TTAAGCTCAT TCTTACCAAA 
TATTCCTCCA GAGATCTTCA AAATTTAATG TCCTGGCGGT TCATAATGGA TCTTGTAAGC 
AGCCTCAGCC GAAACTACAA GGAGTCCCGA AATGCTTTCC GCAAGGCCCT TTATGGCACA 
ACATCAGAAA CAGCAACATG GAGACGTTGT GCGAACTATG TCAATGGGAA TATGGAAAAT 
GCTGTGGGAA GGCTGTACGT GGAAGCAGCA TTTGCTGGAG AGAGTAAACA TGTGGTTGAG 
GATTTGATTA CACAGATCCG TGCAGTTTTT ATTCAGACTT TAGATGACCT CACTTGGATG 
GATGCTGAAA CGAAAAAGAA AGCTGAGGAA AAGGCCTTAG CAATTAAAGA AAGAATTGGC 
TATCCTGATG ACATCATTTC AAATGATAGT AAACTGGATA ATGAATACCT GGAGTTGAAC 
TACAGGGAAG ATGAATACTT TGAAAACATA ATTCAAAACT TGAAGTTCAG CCAAAATAAA 
CAGCTCAAGA AGCTCCGAGA AAAAGTGGAC AAGGATGAGT GGATAAGCGG AGCAGCTGTA 
GTCAATGCAT TTTATTCTTC AGGCAGAAAT CAGATAGTCT TCCCAGCTGG CATTCTGCAG 
CCCCCCTTCT TCAGTGCCCA GCAGTCTAAC TCCTTGAACT ACGGGGGTAT CGGCATGGTC 
ATAGGACACG AAATCACCCA TGGCTTCGAT GACAATGGCA GAAATTTTAA CAAGGATGGA 
GACCTCGTTG ACTGGTGGAC TCAACAGTCG GCAAATAATT TTAAAGACCA ATCCCAATGC 
ATGGTGTACC AGTATGGAAA CTTCTCCTGG GACCTAGCAG GTGGACAGCA TCTCAATGGG 
ATTAATACAC TGGGAGAAAA TATCGCTGAT AATGGAGGTA TTGGCCAAGC ATACAGAGCC 
TATCAAAATT ATGTTAAAAA GAACGGTGAA GAAAAACTAC TTCCTGGACT TGACCTAAAT 
CACAAACAGC TGTTCTTCCT CAACTTTGCC CAGGTGTGGT GTGGGACCTA CAGGCCAGAG 
TATGCCGTCA ACTCCATTAA AACAGATGTT CACAGTCCAG GGAATTTCAG GATTATTGGG 
ACTTTGCAGA ACTCCCCTGA GTTTTCAGAG GCCTTCCATT GCCGCAAGAA TTCATACATG 
AATCCAGAAA AGAAATGCCG AGTTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MME ( XM_005634591.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634648.1 Canis lupus familiaris membrane metallo-endopeptidase (MME), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe24333
Clone ID Related Accession (Same CDS sequence)	XM_022408385.1 , XM_005634590.2 , XM_005634591.2 , XM_005634590.1 , XM_005634591.3
Accession Version	XM_005634591.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	neprilysin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634591.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA GCACTCCAAG GCCAAAGAAG 
CAACGATGGA CCTCCCTGGA GATCAGTCTC TCCGTCCTTG TCCTGCTCCT TACCATCATC 
GCCGTGACAA TGATCGCACT CTATGCAACC TATGATGATG GTATTTGCAA GTCATCAGAC 
TGCATAAAAT CAGCCGCTCG ACTGATCCAG AACATGGATG CCACGGCTGA GCCCTGTACA 
GACTTTTTTA AATACGCCTG TGGAGGCTGG TTGAAACGCA ACGTCATTCC TGAGACTAGC 
TCCCGTTACA GTAACTTTGA CATTTTAAGA GATGAACTTG AAGTCGTTTT GAAAGATGTT 
CTTCAGGAAC CCAAAACTGA GGATATAGTA GCAGTGCAGA AAGCAAAAAC GTTGTACAGA 
TCTTGTATAA ATGAATCTGC TATTGATAGC AGAGGCGGAC AACCTCTGCT CAGTCTGTTA 
CCAGATATCT ATGACTGGCC AGTAGCAACG GATAATTGGG AGCAAACATA TGGTACTTCT 
TGGACAGCTG AGAAGTCTAT TGCACAACTG AATTCTAAAT ATGGGAAAAA AGTCATTCTT 
AATTTTTTTG TTGGCACGGA TGATAAGAAC TCTGTGAACC ATATAATTCA TATTGACCAG 
CCTCGACTTG GCCTCCCGTC CAGAGACTAC TATGTCTGCA CTGGAATATA TGAAGAGGCT 
TGCACAGCGT ATAAAGATTT TATGATTTCT GTGGCCAGAT TGATTCGTAA GGAGAAAGGA 
CTGCTCATCG ATGAAAACCA GCTTTCTTTG GAAATGAACA GAGTTATGGA ATTGGAAAAA 
GAAATTGCCA GTGCTACAAC TAAACCTGAA GATCGAAATG ATCCCATGCT TCTTTATAAC 
AAAATGACAT TAGCCCAGAT CCAAAATAAC TTTACACTAG AGATTGATGG GAAGCCATTC 
AGCTGGTCAA ATTTCACAAA TGAAATCATG TCAACTGTGA ATATTAATAT TCCAAATGAG 
GAAGAAGTGG TCGTTTATGC TCCAGAATAT TTAACCAAAC TTAAGCTCAT TCTTACCAAA 
TATTCCTCCA GAGATCTTCA AAATTTAATG TCCTGGCGGT TCATAATGGA TCTTGTAAGC 
AGCCTCAGCC GAAACTACAA GGAGTCCCGA AATGCTTTCC GCAAGGCCCT TTATGGCACA 
ACATCAGAAA CAGCAACATG GAGACGTTGT GCGAACTATG TCAATGGGAA TATGGAAAAT 
GCTGTGGGAA GGCTGTACGT GGAAGCAGCA TTTGCTGGAG AGAGTAAACA TGTGGTTGAG 
GATTTGATTA CACAGATCCG TGCAGTTTTT ATTCAGACTT TAGATGACCT CACTTGGATG 
GATGCTGAAA CGAAAAAGAA AGCTGAGGAA AAGGCCTTAG CAATTAAAGA AAGAATTGGC 
TATCCTGATG ACATCATTTC AAATGATAGT AAACTGGATA ATGAATACCT GGAGTTGAAC 
TACAGGGAAG ATGAATACTT TGAAAACATA ATTCAAAACT TGAAGTTCAG CCAAAATAAA 
CAGCTCAAGA AGCTCCGAGA AAAAGTGGAC AAGGATGAGT GGATAAGCGG AGCAGCTGTA 
GTCAATGCAT TTTATTCTTC AGGCAGAAAT CAGATAGTCT TCCCAGCTGG CATTCTGCAG 
CCCCCCTTCT TCAGTGCCCA GCAGTCTAAC TCCTTGAACT ACGGGGGTAT CGGCATGGTC 
ATAGGACACG AAATCACCCA TGGCTTCGAT GACAATGGCA GAAATTTTAA CAAGGATGGA 
GACCTCGTTG ACTGGTGGAC TCAACAGTCG GCAAATAATT TTAAAGACCA ATCCCAATGC 
ATGGTGTACC AGTATGGAAA CTTCTCCTGG GACCTAGCAG GTGGACAGCA TCTCAATGGG 
ATTAATACAC TGGGAGAAAA TATCGCTGAT AATGGAGGTA TTGGCCAAGC ATACAGAGCC 
TATCAAAATT ATGTTAAAAA GAACGGTGAA GAAAAACTAC TTCCTGGACT TGACCTAAAT 
CACAAACAGC TGTTCTTCCT CAACTTTGCC CAGGTGTGGT GTGGGACCTA CAGGCCAGAG 
TATGCCGTCA ACTCCATTAA AACAGATGTT CACAGTCCAG GGAATTTCAG GATTATTGGG 
ACTTTGCAGA ACTCCCCTGA GTTTTCAGAG GCCTTCCATT GCCGCAAGAA TTCATACATG 
AATCCAGAAA AGAAATGCCG AGTTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634648.1
CDS	90..2339
Translation	MGRSESQMDITDISTPRPKKQRWTSLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDATAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLSLLPDIYDWPVATDNWEQTYGTSWTAEKSIAQLNSKYGKKVILNFFVGTDDKNSVNHIIHIDQPRLGLPSRDYYVCTGIYEEACTAYKDFMISVARLIRKEKGLLIDENQLSLEMNRVMELEKEIASATTKPEDRNDPMLLYNKMTLAQIQNNFTLEIDGKPFSWSNFTNEIMSTVNINIPNEEEVVVYAPEYLTKLKLILTKYSSRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLITQIRAVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNDSKLDNEYLELNYREDEYFENIIQNLKFSQNKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSPEFSEAFHCRKNSYMNPEKKCRVW

Target ORF information:

RefSeq Version	XM_005634591.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris membrane metallo-endopeptidase (MME), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634591.2

ORF Insert Sequence:

ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA GCACTCCAAG GCCAAAGAAG 
CAACGATGGA CCTCCCTGGA GATCAGTCTC TCCGTCCTTG TCCTGCTCCT TACCATCATC 
GCCGTGACAA TGATCGCACT CTATGCAACC TATGATGATG GTATTTGCAA GTCATCAGAC 
TGCATAAAAT CAGCCGCTCG ACTGATCCAG AACATGGATG CCACGGCTGA GCCCTGTACA 
GACTTTTTTA AATACGCCTG TGGAGGCTGG TTGAAACGCA ACGTCATTCC TGAGACTAGC 
TCCCGTTACA GTAACTTTGA CATTTTAAGA GATGAACTTG AAGTCGTTTT GAAAGATGTT 
CTTCAGGAAC CCAAAACTGA GGATATAGTA GCAGTGCAGA AAGCAAAAAC GTTGTACAGA 
TCTTGTATAA ATGAATCTGC TATTGATAGC AGAGGCGGAC AACCTCTGCT CAGTCTGTTA 
CCAGATATCT ATGACTGGCC AGTAGCAACG GATAATTGGG AGCAAACATA TGGTACTTCT 
TGGACAGCTG AGAAGTCTAT TGCACAACTG AATTCTAAAT ATGGGAAAAA AGTCATTCTT 
AATTTTTTTG TTGGCACGGA TGATAAGAAC TCTGTGAACC ATATAATTCA TATTGACCAG 
CCTCGACTTG GCCTCCCGTC CAGAGACTAC TATGTCTGCA CTGGAATATA TGAAGAGGCT 
TGCACAGCGT ATAAAGATTT TATGATTTCT GTGGCCAGAT TGATTCGTAA GGAGAAAGGA 
CTGCTCATCG ATGAAAACCA GCTTTCTTTG GAAATGAACA GAGTTATGGA ATTGGAAAAA 
GAAATTGCCA GTGCTACAAC TAAACCTGAA GATCGAAATG ATCCCATGCT TCTTTATAAC 
AAAATGACAT TAGCCCAGAT CCAAAATAAC TTTACACTAG AGATTGATGG GAAGCCATTC 
AGCTGGTCAA ATTTCACAAA TGAAATCATG TCAACTGTGA ATATTAATAT TCCAAATGAG 
GAAGAAGTGG TCGTTTATGC TCCAGAATAT TTAACCAAAC TTAAGCTCAT TCTTACCAAA 
TATTCCTCCA GAGATCTTCA AAATTTAATG TCCTGGCGGT TCATAATGGA TCTTGTAAGC 
AGCCTCAGCC GAAACTACAA GGAGTCCCGA AATGCTTTCC GCAAGGCCCT TTATGGCACA 
ACATCAGAAA CAGCAACATG GAGACGTTGT GCGAACTATG TCAATGGGAA TATGGAAAAT 
GCTGTGGGAA GGCTGTACGT GGAAGCAGCA TTTGCTGGAG AGAGTAAACA TGTGGTTGAG 
GATTTGATTA CACAGATCCG TGCAGTTTTT ATTCAGACTT TAGATGACCT CACTTGGATG 
GATGCTGAAA CGAAAAAGAA AGCTGAGGAA AAGGCCTTAG CAATTAAAGA AAGAATTGGC 
TATCCTGATG ACATCATTTC AAATGATAGT AAACTGGATA ATGAATACCT GGAGTTGAAC 
TACAGGGAAG ATGAATACTT TGAAAACATA ATTCAAAACT TGAAGTTCAG CCAAAATAAA 
CAGCTCAAGA AGCTCCGAGA AAAAGTGGAC AAGGATGAGT GGATAAGCGG AGCAGCTGTA 
GTCAATGCAT TTTATTCTTC AGGCAGAAAT CAGATAGTCT TCCCAGCTGG CATTCTGCAG 
CCCCCCTTCT TCAGTGCCCA GCAGTCTAAC TCCTTGAACT ACGGGGGTAT CGGCATGGTC 
ATAGGACACG AAATCACCCA TGGCTTCGAT GACAATGGCA GAAATTTTAA CAAGGATGGA 
GACCTCGTTG ACTGGTGGAC TCAACAGTCG GCAAATAATT TTAAAGACCA ATCCCAATGC 
ATGGTGTACC AGTATGGAAA CTTCTCCTGG GACCTAGCAG GTGGACAGCA TCTCAATGGG 
ATTAATACAC TGGGAGAAAA TATCGCTGAT AATGGAGGTA TTGGCCAAGC ATACAGAGCC 
TATCAAAATT ATGTTAAAAA GAACGGTGAA GAAAAACTAC TTCCTGGACT TGACCTAAAT 
CACAAACAGC TGTTCTTCCT CAACTTTGCC CAGGTGTGGT GTGGGACCTA CAGGCCAGAG 
TATGCCGTCA ACTCCATTAA AACAGATGTT CACAGTCCAG GGAATTTCAG GATTATTGGG 
ACTTTGCAGA ACTCCCCTGA GTTTTCAGAG GCCTTCCATT GCCGCAAGAA TTCATACATG 
AATCCAGAAA AGAAATGCCG AGTTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MME ( XM_005634590.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634647.1 Canis lupus familiaris membrane metallo-endopeptidase (MME), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe24333
Clone ID Related Accession (Same CDS sequence)	XM_022408385.1 , XM_005634590.2 , XM_005634591.2 , XM_005634590.1 , XM_005634591.3
Accession Version	XM_005634590.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	neprilysin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA GCACTCCAAG GCCAAAGAAG 
CAACGATGGA CCTCCCTGGA GATCAGTCTC TCCGTCCTTG TCCTGCTCCT TACCATCATC 
GCCGTGACAA TGATCGCACT CTATGCAACC TATGATGATG GTATTTGCAA GTCATCAGAC 
TGCATAAAAT CAGCCGCTCG ACTGATCCAG AACATGGATG CCACGGCTGA GCCCTGTACA 
GACTTTTTTA AATACGCCTG TGGAGGCTGG TTGAAACGCA ACGTCATTCC TGAGACTAGC 
TCCCGTTACA GTAACTTTGA CATTTTAAGA GATGAACTTG AAGTCGTTTT GAAAGATGTT 
CTTCAGGAAC CCAAAACTGA GGATATAGTA GCAGTGCAGA AAGCAAAAAC GTTGTACAGA 
TCTTGTATAA ATGAATCTGC TATTGATAGC AGAGGCGGAC AACCTCTGCT CAGTCTGTTA 
CCAGATATCT ATGACTGGCC AGTAGCAACG GATAATTGGG AGCAAACATA TGGTACTTCT 
TGGACAGCTG AGAAGTCTAT TGCACAACTG AATTCTAAAT ATGGGAAAAA AGTCATTCTT 
AATTTTTTTG TTGGCACGGA TGATAAGAAC TCTGTGAACC ATATAATTCA TATTGACCAG 
CCTCGACTTG GCCTCCCGTC CAGAGACTAC TATGTCTGCA CTGGAATATA TGAAGAGGCT 
TGCACAGCGT ATAAAGATTT TATGATTTCT GTGGCCAGAT TGATTCGTAA GGAGAAAGGA 
CTGCTCATCG ATGAAAACCA GCTTTCTTTG GAAATGAACA GAGTTATGGA ATTGGAAAAA 
GAAATTGCCA GTGCTACAAC TAAACCTGAA GATCGAAATG ATCCCATGCT TCTTTATAAC 
AAAATGACAT TAGCCCAGAT CCAAAATAAC TTTACACTAG AGATTGATGG GAAGCCATTC 
AGCTGGTCAA ATTTCACAAA TGAAATCATG TCAACTGTGA ATATTAATAT TCCAAATGAG 
GAAGAAGTGG TCGTTTATGC TCCAGAATAT TTAACCAAAC TTAAGCTCAT TCTTACCAAA 
TATTCCTCCA GAGATCTTCA AAATTTAATG TCCTGGCGGT TCATAATGGA TCTTGTAAGC 
AGCCTCAGCC GAAACTACAA GGAGTCCCGA AATGCTTTCC GCAAGGCCCT TTATGGCACA 
ACATCAGAAA CAGCAACATG GAGACGTTGT GCGAACTATG TCAATGGGAA TATGGAAAAT 
GCTGTGGGAA GGCTGTACGT GGAAGCAGCA TTTGCTGGAG AGAGTAAACA TGTGGTTGAG 
GATTTGATTA CACAGATCCG TGCAGTTTTT ATTCAGACTT TAGATGACCT CACTTGGATG 
GATGCTGAAA CGAAAAAGAA AGCTGAGGAA AAGGCCTTAG CAATTAAAGA AAGAATTGGC 
TATCCTGATG ACATCATTTC AAATGATAGT AAACTGGATA ATGAATACCT GGAGTTGAAC 
TACAGGGAAG ATGAATACTT TGAAAACATA ATTCAAAACT TGAAGTTCAG CCAAAATAAA 
CAGCTCAAGA AGCTCCGAGA AAAAGTGGAC AAGGATGAGT GGATAAGCGG AGCAGCTGTA 
GTCAATGCAT TTTATTCTTC AGGCAGAAAT CAGATAGTCT TCCCAGCTGG CATTCTGCAG 
CCCCCCTTCT TCAGTGCCCA GCAGTCTAAC TCCTTGAACT ACGGGGGTAT CGGCATGGTC 
ATAGGACACG AAATCACCCA TGGCTTCGAT GACAATGGCA GAAATTTTAA CAAGGATGGA 
GACCTCGTTG ACTGGTGGAC TCAACAGTCG GCAAATAATT TTAAAGACCA ATCCCAATGC 
ATGGTGTACC AGTATGGAAA CTTCTCCTGG GACCTAGCAG GTGGACAGCA TCTCAATGGG 
ATTAATACAC TGGGAGAAAA TATCGCTGAT AATGGAGGTA TTGGCCAAGC ATACAGAGCC 
TATCAAAATT ATGTTAAAAA GAACGGTGAA GAAAAACTAC TTCCTGGACT TGACCTAAAT 
CACAAACAGC TGTTCTTCCT CAACTTTGCC CAGGTGTGGT GTGGGACCTA CAGGCCAGAG 
TATGCCGTCA ACTCCATTAA AACAGATGTT CACAGTCCAG GGAATTTCAG GATTATTGGG 
ACTTTGCAGA ACTCCCCTGA GTTTTCAGAG GCCTTCCATT GCCGCAAGAA TTCATACATG 
AATCCAGAAA AGAAATGCCG AGTTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634647.1
CDS	116..2365
Translation	MGRSESQMDITDISTPRPKKQRWTSLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDATAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLSLLPDIYDWPVATDNWEQTYGTSWTAEKSIAQLNSKYGKKVILNFFVGTDDKNSVNHIIHIDQPRLGLPSRDYYVCTGIYEEACTAYKDFMISVARLIRKEKGLLIDENQLSLEMNRVMELEKEIASATTKPEDRNDPMLLYNKMTLAQIQNNFTLEIDGKPFSWSNFTNEIMSTVNINIPNEEEVVVYAPEYLTKLKLILTKYSSRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLITQIRAVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNDSKLDNEYLELNYREDEYFENIIQNLKFSQNKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSPEFSEAFHCRKNSYMNPEKKCRVW

Target ORF information:

RefSeq Version	XM_005634590.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris membrane metallo-endopeptidase (MME), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634590.1

ORF Insert Sequence:

ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA GCACTCCAAG GCCAAAGAAG 
CAACGATGGA CCTCCCTGGA GATCAGTCTC TCCGTCCTTG TCCTGCTCCT TACCATCATC 
GCCGTGACAA TGATCGCACT CTATGCAACC TATGATGATG GTATTTGCAA GTCATCAGAC 
TGCATAAAAT CAGCCGCTCG ACTGATCCAG AACATGGATG CCACGGCTGA GCCCTGTACA 
GACTTTTTTA AATACGCCTG TGGAGGCTGG TTGAAACGCA ACGTCATTCC TGAGACTAGC 
TCCCGTTACA GTAACTTTGA CATTTTAAGA GATGAACTTG AAGTCGTTTT GAAAGATGTT 
CTTCAGGAAC CCAAAACTGA GGATATAGTA GCAGTGCAGA AAGCAAAAAC GTTGTACAGA 
TCTTGTATAA ATGAATCTGC TATTGATAGC AGAGGCGGAC AACCTCTGCT CAGTCTGTTA 
CCAGATATCT ATGACTGGCC AGTAGCAACG GATAATTGGG AGCAAACATA TGGTACTTCT 
TGGACAGCTG AGAAGTCTAT TGCACAACTG AATTCTAAAT ATGGGAAAAA AGTCATTCTT 
AATTTTTTTG TTGGCACGGA TGATAAGAAC TCTGTGAACC ATATAATTCA TATTGACCAG 
CCTCGACTTG GCCTCCCGTC CAGAGACTAC TATGTCTGCA CTGGAATATA TGAAGAGGCT 
TGCACAGCGT ATAAAGATTT TATGATTTCT GTGGCCAGAT TGATTCGTAA GGAGAAAGGA 
CTGCTCATCG ATGAAAACCA GCTTTCTTTG GAAATGAACA GAGTTATGGA ATTGGAAAAA 
GAAATTGCCA GTGCTACAAC TAAACCTGAA GATCGAAATG ATCCCATGCT TCTTTATAAC 
AAAATGACAT TAGCCCAGAT CCAAAATAAC TTTACACTAG AGATTGATGG GAAGCCATTC 
AGCTGGTCAA ATTTCACAAA TGAAATCATG TCAACTGTGA ATATTAATAT TCCAAATGAG 
GAAGAAGTGG TCGTTTATGC TCCAGAATAT TTAACCAAAC TTAAGCTCAT TCTTACCAAA 
TATTCCTCCA GAGATCTTCA AAATTTAATG TCCTGGCGGT TCATAATGGA TCTTGTAAGC 
AGCCTCAGCC GAAACTACAA GGAGTCCCGA AATGCTTTCC GCAAGGCCCT TTATGGCACA 
ACATCAGAAA CAGCAACATG GAGACGTTGT GCGAACTATG TCAATGGGAA TATGGAAAAT 
GCTGTGGGAA GGCTGTACGT GGAAGCAGCA TTTGCTGGAG AGAGTAAACA TGTGGTTGAG 
GATTTGATTA CACAGATCCG TGCAGTTTTT ATTCAGACTT TAGATGACCT CACTTGGATG 
GATGCTGAAA CGAAAAAGAA AGCTGAGGAA AAGGCCTTAG CAATTAAAGA AAGAATTGGC 
TATCCTGATG ACATCATTTC AAATGATAGT AAACTGGATA ATGAATACCT GGAGTTGAAC 
TACAGGGAAG ATGAATACTT TGAAAACATA ATTCAAAACT TGAAGTTCAG CCAAAATAAA 
CAGCTCAAGA AGCTCCGAGA AAAAGTGGAC AAGGATGAGT GGATAAGCGG AGCAGCTGTA 
GTCAATGCAT TTTATTCTTC AGGCAGAAAT CAGATAGTCT TCCCAGCTGG CATTCTGCAG 
CCCCCCTTCT TCAGTGCCCA GCAGTCTAAC TCCTTGAACT ACGGGGGTAT CGGCATGGTC 
ATAGGACACG AAATCACCCA TGGCTTCGAT GACAATGGCA GAAATTTTAA CAAGGATGGA 
GACCTCGTTG ACTGGTGGAC TCAACAGTCG GCAAATAATT TTAAAGACCA ATCCCAATGC 
ATGGTGTACC AGTATGGAAA CTTCTCCTGG GACCTAGCAG GTGGACAGCA TCTCAATGGG 
ATTAATACAC TGGGAGAAAA TATCGCTGAT AATGGAGGTA TTGGCCAAGC ATACAGAGCC 
TATCAAAATT ATGTTAAAAA GAACGGTGAA GAAAAACTAC TTCCTGGACT TGACCTAAAT 
CACAAACAGC TGTTCTTCCT CAACTTTGCC CAGGTGTGGT GTGGGACCTA CAGGCCAGAG 
TATGCCGTCA ACTCCATTAA AACAGATGTT CACAGTCCAG GGAATTTCAG GATTATTGGG 
ACTTTGCAGA ACTCCCCTGA GTTTTCAGAG GCCTTCCATT GCCGCAAGAA TTCATACATG 
AATCCAGAAA AGAAATGCCG AGTTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MME ( XM_005634591.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634648.1 Canis lupus familiaris membrane metalloendopeptidase (MME), transcript variant X2, mRNA.

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CloneID	OCe24333
Clone ID Related Accession (Same CDS sequence)	XM_022408385.1 , XM_005634590.2 , XM_005634591.2 , XM_005634590.1 , XM_005634591.3
Accession Version	XM_005634591.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	neprilysin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634591.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA GCACTCCAAG GCCAAAGAAG 
CAACGATGGA CCTCCCTGGA GATCAGTCTC TCCGTCCTTG TCCTGCTCCT TACCATCATC 
GCCGTGACAA TGATCGCACT CTATGCAACC TATGATGATG GTATTTGCAA GTCATCAGAC 
TGCATAAAAT CAGCCGCTCG ACTGATCCAG AACATGGATG CCACGGCTGA GCCCTGTACA 
GACTTTTTTA AATACGCCTG TGGAGGCTGG TTGAAACGCA ACGTCATTCC TGAGACTAGC 
TCCCGTTACA GTAACTTTGA CATTTTAAGA GATGAACTTG AAGTCGTTTT GAAAGATGTT 
CTTCAGGAAC CCAAAACTGA GGATATAGTA GCAGTGCAGA AAGCAAAAAC GTTGTACAGA 
TCTTGTATAA ATGAATCTGC TATTGATAGC AGAGGCGGAC AACCTCTGCT CAGTCTGTTA 
CCAGATATCT ATGACTGGCC AGTAGCAACG GATAATTGGG AGCAAACATA TGGTACTTCT 
TGGACAGCTG AGAAGTCTAT TGCACAACTG AATTCTAAAT ATGGGAAAAA AGTCATTCTT 
AATTTTTTTG TTGGCACGGA TGATAAGAAC TCTGTGAACC ATATAATTCA TATTGACCAG 
CCTCGACTTG GCCTCCCGTC CAGAGACTAC TATGTCTGCA CTGGAATATA TGAAGAGGCT 
TGCACAGCGT ATAAAGATTT TATGATTTCT GTGGCCAGAT TGATTCGTAA GGAGAAAGGA 
CTGCTCATCG ATGAAAACCA GCTTTCTTTG GAAATGAACA GAGTTATGGA ATTGGAAAAA 
GAAATTGCCA GTGCTACAAC TAAACCTGAA GATCGAAATG ATCCCATGCT TCTTTATAAC 
AAAATGACAT TAGCCCAGAT CCAAAATAAC TTTACACTAG AGATTGATGG GAAGCCATTC 
AGCTGGTCAA ATTTCACAAA TGAAATCATG TCAACTGTGA ATATTAATAT TCCAAATGAG 
GAAGAAGTGG TCGTTTATGC TCCAGAATAT TTAACCAAAC TTAAGCTCAT TCTTACCAAA 
TATTCCTCCA GAGATCTTCA AAATTTAATG TCCTGGCGGT TCATAATGGA TCTTGTAAGC 
AGCCTCAGCC GAAACTACAA GGAGTCCCGA AATGCTTTCC GCAAGGCCCT TTATGGCACA 
ACATCAGAAA CAGCAACATG GAGACGTTGT GCGAACTATG TCAATGGGAA TATGGAAAAT 
GCTGTGGGAA GGCTGTACGT GGAAGCAGCA TTTGCTGGAG AGAGTAAACA TGTGGTTGAG 
GATTTGATTA CACAGATCCG TGCAGTTTTT ATTCAGACTT TAGATGACCT CACTTGGATG 
GATGCTGAAA CGAAAAAGAA AGCTGAGGAA AAGGCCTTAG CAATTAAAGA AAGAATTGGC 
TATCCTGATG ACATCATTTC AAATGATAGT AAACTGGATA ATGAATACCT GGAGTTGAAC 
TACAGGGAAG ATGAATACTT TGAAAACATA ATTCAAAACT TGAAGTTCAG CCAAAATAAA 
CAGCTCAAGA AGCTCCGAGA AAAAGTGGAC AAGGATGAGT GGATAAGCGG AGCAGCTGTA 
GTCAATGCAT TTTATTCTTC AGGCAGAAAT CAGATAGTCT TCCCAGCTGG CATTCTGCAG 
CCCCCCTTCT TCAGTGCCCA GCAGTCTAAC TCCTTGAACT ACGGGGGTAT CGGCATGGTC 
ATAGGACACG AAATCACCCA TGGCTTCGAT GACAATGGCA GAAATTTTAA CAAGGATGGA 
GACCTCGTTG ACTGGTGGAC TCAACAGTCG GCAAATAATT TTAAAGACCA ATCCCAATGC 
ATGGTGTACC AGTATGGAAA CTTCTCCTGG GACCTAGCAG GTGGACAGCA TCTCAATGGG 
ATTAATACAC TGGGAGAAAA TATCGCTGAT AATGGAGGTA TTGGCCAAGC ATACAGAGCC 
TATCAAAATT ATGTTAAAAA GAACGGTGAA GAAAAACTAC TTCCTGGACT TGACCTAAAT 
CACAAACAGC TGTTCTTCCT CAACTTTGCC CAGGTGTGGT GTGGGACCTA CAGGCCAGAG 
TATGCCGTCA ACTCCATTAA AACAGATGTT CACAGTCCAG GGAATTTCAG GATTATTGGG 
ACTTTGCAGA ACTCCCCTGA GTTTTCAGAG GCCTTCCATT GCCGCAAGAA TTCATACATG 
AATCCAGAAA AGAAATGCCG AGTTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634648.1
CDS	124..2373
Translation	MGRSESQMDITDISTPRPKKQRWTSLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDATAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLSLLPDIYDWPVATDNWEQTYGTSWTAEKSIAQLNSKYGKKVILNFFVGTDDKNSVNHIIHIDQPRLGLPSRDYYVCTGIYEEACTAYKDFMISVARLIRKEKGLLIDENQLSLEMNRVMELEKEIASATTKPEDRNDPMLLYNKMTLAQIQNNFTLEIDGKPFSWSNFTNEIMSTVNINIPNEEEVVVYAPEYLTKLKLILTKYSSRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLITQIRAVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNDSKLDNEYLELNYREDEYFENIIQNLKFSQNKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSPEFSEAFHCRKNSYMNPEKKCRVW

Target ORF information:

RefSeq Version	XM_005634591.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris membrane metalloendopeptidase (MME), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634591.3

ORF Insert Sequence:

ATGGGCAGAT CAGAAAGTCA GATGGATATA ACTGATATCA GCACTCCAAG GCCAAAGAAG 
CAACGATGGA CCTCCCTGGA GATCAGTCTC TCCGTCCTTG TCCTGCTCCT TACCATCATC 
GCCGTGACAA TGATCGCACT CTATGCAACC TATGATGATG GTATTTGCAA GTCATCAGAC 
TGCATAAAAT CAGCCGCTCG ACTGATCCAG AACATGGATG CCACGGCTGA GCCCTGTACA 
GACTTTTTTA AATACGCCTG TGGAGGCTGG TTGAAACGCA ACGTCATTCC TGAGACTAGC 
TCCCGTTACA GTAACTTTGA CATTTTAAGA GATGAACTTG AAGTCGTTTT GAAAGATGTT 
CTTCAGGAAC CCAAAACTGA GGATATAGTA GCAGTGCAGA AAGCAAAAAC GTTGTACAGA 
TCTTGTATAA ATGAATCTGC TATTGATAGC AGAGGCGGAC AACCTCTGCT CAGTCTGTTA 
CCAGATATCT ATGACTGGCC AGTAGCAACG GATAATTGGG AGCAAACATA TGGTACTTCT 
TGGACAGCTG AGAAGTCTAT TGCACAACTG AATTCTAAAT ATGGGAAAAA AGTCATTCTT 
AATTTTTTTG TTGGCACGGA TGATAAGAAC TCTGTGAACC ATATAATTCA TATTGACCAG 
CCTCGACTTG GCCTCCCGTC CAGAGACTAC TATGTCTGCA CTGGAATATA TGAAGAGGCT 
TGCACAGCGT ATAAAGATTT TATGATTTCT GTGGCCAGAT TGATTCGTAA GGAGAAAGGA 
CTGCTCATCG ATGAAAACCA GCTTTCTTTG GAAATGAACA GAGTTATGGA ATTGGAAAAA 
GAAATTGCCA GTGCTACAAC TAAACCTGAA GATCGAAATG ATCCCATGCT TCTTTATAAC 
AAAATGACAT TAGCCCAGAT CCAAAATAAC TTTACACTAG AGATTGATGG GAAGCCATTC 
AGCTGGTCAA ATTTCACAAA TGAAATCATG TCAACTGTGA ATATTAATAT TCCAAATGAG 
GAAGAAGTGG TCGTTTATGC TCCAGAATAT TTAACCAAAC TTAAGCTCAT TCTTACCAAA 
TATTCCTCCA GAGATCTTCA AAATTTAATG TCCTGGCGGT TCATAATGGA TCTTGTAAGC 
AGCCTCAGCC GAAACTACAA GGAGTCCCGA AATGCTTTCC GCAAGGCCCT TTATGGCACA 
ACATCAGAAA CAGCAACATG GAGACGTTGT GCGAACTATG TCAATGGGAA TATGGAAAAT 
GCTGTGGGAA GGCTGTACGT GGAAGCAGCA TTTGCTGGAG AGAGTAAACA TGTGGTTGAG 
GATTTGATTA CACAGATCCG TGCAGTTTTT ATTCAGACTT TAGATGACCT CACTTGGATG 
GATGCTGAAA CGAAAAAGAA AGCTGAGGAA AAGGCCTTAG CAATTAAAGA AAGAATTGGC 
TATCCTGATG ACATCATTTC AAATGATAGT AAACTGGATA ATGAATACCT GGAGTTGAAC 
TACAGGGAAG ATGAATACTT TGAAAACATA ATTCAAAACT TGAAGTTCAG CCAAAATAAA 
CAGCTCAAGA AGCTCCGAGA AAAAGTGGAC AAGGATGAGT GGATAAGCGG AGCAGCTGTA 
GTCAATGCAT TTTATTCTTC AGGCAGAAAT CAGATAGTCT TCCCAGCTGG CATTCTGCAG 
CCCCCCTTCT TCAGTGCCCA GCAGTCTAAC TCCTTGAACT ACGGGGGTAT CGGCATGGTC 
ATAGGACACG AAATCACCCA TGGCTTCGAT GACAATGGCA GAAATTTTAA CAAGGATGGA 
GACCTCGTTG ACTGGTGGAC TCAACAGTCG GCAAATAATT TTAAAGACCA ATCCCAATGC 
ATGGTGTACC AGTATGGAAA CTTCTCCTGG GACCTAGCAG GTGGACAGCA TCTCAATGGG 
ATTAATACAC TGGGAGAAAA TATCGCTGAT AATGGAGGTA TTGGCCAAGC ATACAGAGCC 
TATCAAAATT ATGTTAAAAA GAACGGTGAA GAAAAACTAC TTCCTGGACT TGACCTAAAT 
CACAAACAGC TGTTCTTCCT CAACTTTGCC CAGGTGTGGT GTGGGACCTA CAGGCCAGAG 
TATGCCGTCA ACTCCATTAA AACAGATGTT CACAGTCCAG GGAATTTCAG GATTATTGGG 
ACTTTGCAGA ACTCCCCTGA GTTTTCAGAG GCCTTCCATT GCCGCAAGAA TTCATACATG 
AATCCAGAAA AGAAATGCCG AGTTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Temporal changes in neutral endopeptidase/CD10 immunoexpression in the cyclic and early pregnant canine endometrium.
Theriogenology82(6)815-26(2014 Oct)
Payan-Carreira R,Santos C,Miranda S,Pereira RM,Santos D,Pires MA

Use of CD10 as a marker of canine mammary myoepithelial cells.
Veterinary journal (London, England : 1997)195(2)192-9(2013 Feb)
S?nchez-C?spedes R,Su?rez-Bonnet A,Mill?n Y,Guil-Luna S,Reymundo C,Herr?ez P,Espinosa de Los Monteros A,Martin de Las Mulas J

Cloning, sequencing and expression in the dog of the main amyloid precursor protein isoforms and some of the enzymes related with their processing.
Neuroscience171(4)1091-101(2010 Dec)
Sarasa L,Gallego C,Monle?n I,Olvera A,Canudas J,Monta??s M,Pesini P,Sarasa M

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MME cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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