Peg10 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Peg10
Entrez Gene ID	170676
Full Name	paternally expressed 10
Synonyms	AA407948,Edr,HB-1,MEF3L,Mar2,Mart2,MyEF-3,Rtl2
General protein information	Preferred Names paternally expressed 10 Names retrotransposon-derived protein PEG10 MEF3-like protein 1 MyEF-3 like embryonal carcinoma differentiation regulated protein mammalian retrotransposon-derived protein 2 myelin expression factor 3 myelin expression factor 3-like protein 1 paternally expressed gene 10 protein retrotransposon gag domain-containing protein 3 retrotransposon-derived gag-like polyprotein ty3/Gypsy-like protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 A1\|6 1.81 cM
Summary	This is a paternally expressed imprinted gene that is thought to have been derived from the Ty3/Gypsy family of retrotransposons. It contains two overlapping open reading frames, RF1 and RF2, and expresses two proteins: a shorter, gag-like protein (with a CCHC-type zinc finger domain) from RF1; and a longer, gag/pol-like fusion protein (with an additional aspartic protease motif) from RF1/RF2 by -1 translational frameshifting (-1 FS). While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements required for -1 FS. It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation, apoptosis and cancer development. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. [provided by RefSeq, Oct 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001040611.1	NP_001035701.1	retrotransposon-derived protein PEG10 isoform 2
NM_130877.2	NP_570947.2	retrotransposon-derived protein PEG10 isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Peg10	NP_570947.2
Canis lupus familiaris (dog)	PEG10	NP_001166014.2
Homo sapiens (human)	PEG10	NP_001165908.1
Pan troglodytes (chimpanzee)	PEG10	NP_001165897.1
	PEG10	NP_001165893.1
Rattus norvegicus (Norway rat)	LOC102553587	XP_006236145.1
Bos taurus (cattle)	PEG10	NP_001120682.1

Gene Ontology
Bibliography

Related articles in PubMed

Mammalian gene PEG10 expresses two reading frames by high efficiency -1 frameshifting in embryonic-associated tissues.
Clark MB, J?nicke M, Gottesb?hren U, Kleffmann T, Legge M, Poole ES, Tate WP
The Journal of biological chemistry282(52)37359-69(2007 Dec)

Deletion of Peg10, an imprinted gene acquired from a retrotransposon, causes early embryonic lethality.
Ono R, Nakamura K, Inoue K, Naruse M, Usami T, Wakisaka-Saito N, Hino T, Suzuki-Migishima R, Ogonuki N, Miki H, Kohda T, Ogura A, Yokoyama M, Kaneko-Ishino T, Ishino F
Nature genetics38(1)101-6(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of DNA methylation at the Peg10 imprinted gDMD associated with decreased embryonic viability and decreased labyrinthine volume.
Title: Partial Loss of Genomic Imprinting Reveals Important Roles for Kcnq1 and Peg10 Imprinted Domains in Placental Development.

Knockdown of Syncytin-A demonstrated a functional regulation of cell-cell fusion, where knockdown of Peg10 showed no involvement in cell fusion
Title: Regulation of murine placentogenesis by the retroviral genes Syncytin-A, Syncytin-B and Peg10.

the ORF1-2 protein of PEG10, synthesized utilizing the most efficient -1 frameshift mechanism yet documented in vivo, will have an essential function that is intrinsic to the importance of PEG10 in mammals
Title: Mammalian gene PEG10 expresses two reading frames by high efficiency -1 frameshifting in embryonic-associated tissues.

It is a retrotransposon-derived imprinted gene and plays a essential role in placental formation.(review)
Title: [Origin of viviparity and genomic imprinting in mammals: a view from a retrotransposon-derived imprinted gene PEG10].

These findings strongly indicate that peg10 plays a crucial role at the immediate early stage of adipocyte differentiation.
Title: peg10, an imprinted gene, plays a crucial role in adipocyte differentiation.

The following Peg10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Peg10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14774	NM_001040611.1 Latest version!	Mus musculus paternally expressed 10 (Peg10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OMu83568	NM_130877.2 Latest version!	Mus musculus paternally expressed 10 (Peg10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Peg10 ( NM_001040611.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001035701.1 Mus musculus paternally expressed 10 (Peg10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14774
Clone ID Related Accession (Same CDS sequence)	NM_001040611.1
Accession Version	NM_001040611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	retrotransposon-derived protein PEG10 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ127881.1, AB091827.1, AC084315.4, CF735227.1 and BC094558.1. Transcript Variant: This variant (1) has two overlapping open reading frames (RFI and RF2). This isoform (2) produced from RF1 in the absence of -1 translational frameshifting has a shorter C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CJ128728.1, BU519162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 12840045 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCTG CGGGTGGTTC CTCCAACTGC CCGCCCCCTC CCCCTCCCCC TCCTCCCAAC 
AACAACAACA ACAACAACAC CCCAAAGAGC CCAGGCGTGC CTGACGCCGA AGATGATGAT 
GAACGCAGAC ACGATGAGCT CCCTGAAGAC ATCAACAACT TTGACGAAGA CATGAACAGG 
CAGTTTGAGA ATATGAACCT GCTGGATCAG GTGGAGTTGC TTGCACAGAG CTACAGTCTG 
CTGGATCATT TAGATGACTT TGATGATGAT GATGAAGACG ATGACTTTGA TCCAGAACCT 
GACCAGGATG AGCTCCCTGA GTACAGTGAC GATGATGACC TGGAGCTTCA GGGTGCTGCA 
GCAGCCCCTA TCCCAAACTT TTTCTCCGAT GATGACTGCC TTGAAGACCT TCCTGAGAAG 
TTCGATGGCA ACCCTGACAT GCTGGGTCCT TTCATGTATC AGTGCCAGCT CTTCATGGAA 
AAGAGCACCA GAGATTTCTC AGTTGACCGC ATCCGTGTGT GCTTCGTGAC AAGCATGCTG 
ATCGGCCGTG CCGCCCGCTG GGCTACTGCC AAGCTGCAAA GATGTACTTA CCTGATGCAC 
AACTACACTG CCTTTATGAT GGAGCTGAAG CATGTCTTTG AAGACCCTCA GAGACGTGAA 
GCTGCCAAAC GCAAGATCAG ACGTCTGCGC CAGGGCCCTG GGCCTGTTGT GGACTACTCC 
AATGCATTCC AGATGATTGC CCAGGACCTG GATTGGACTG AGCCTGCCCT GATGGATCAG 
TTCCAGGAAG GTCTCAACCC AGACATTCGC GCAGAGCTGT CTCGCCAGGA GGCCCCCAAG 
ACCCTGGCTG CTCTGATTAC TGCCTGTATT CACATCGAGA GAAGGCTGGC TCGTGACGCT 
GCTGCAAAGC CCGATCCTTC ACCCAGAGCC TTGGTGATGC CTCCAAACAG CCAGACCGAT 
CCCACCGAGC CTGTGGGAGG TGCCCGCATG CGCCTGTCCA AGGAAGAAAA GGAGAGACGC 
CGCAAAATGA ATTTGTGTCT CTACTGTGGC AATGGAGGCC ATTTCGCCGA CACGTGTCCA 
GCGAAAGCCT CCAAGAATTC GCCGCCGGGA AACTCCCCGG CCCCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035701.1
CDS	371..1501
Translation	MAAAGGSSNCPPPPPPPPPNNNNNNNTPKSPGVPDAEDDDERRHDELPEDINNFDEDMNRQFENMNLLDQVELLAQSYSLLDHLDDFDDDDEDDDFDPEPDQDELPEYSDDDDLELQGAAAAPIPNFFSDDDCLEDLPEKFDGNPDMLGPFMYQCQLFMEKSTRDFSVDRIRVCFVTSMLIGRAARWATAKLQRCTYLMHNYTAFMMELKHVFEDPQRREAAKRKIRRLRQGPGPVVDYSNAFQMIAQDLDWTEPALMDQFQEGLNPDIRAELSRQEAPKTLAALITACIHIERRLARDAAAKPDPSPRALVMPPNSQTDPTEPVGGARMRLSKEEKERRRKMNLCLYCGNGGHFADTCPAKASKNSPPGNSPAPL

Target ORF information:

RefSeq Version	NM_001040611.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus paternally expressed 10 (Peg10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040611.1

ORF Insert Sequence:

ATGGCTGCTG CGGGTGGTTC CTCCAACTGC CCGCCCCCTC CCCCTCCCCC TCCTCCCAAC 
AACAACAACA ACAACAACAC CCCAAAGAGC CCAGGCGTGC CTGACGCCGA AGATGATGAT 
GAACGCAGAC ACGATGAGCT CCCTGAAGAC ATCAACAACT TTGACGAAGA CATGAACAGG 
CAGTTTGAGA ATATGAACCT GCTGGATCAG GTGGAGTTGC TTGCACAGAG CTACAGTCTG 
CTGGATCATT TAGATGACTT TGATGATGAT GATGAAGACG ATGACTTTGA TCCAGAACCT 
GACCAGGATG AGCTCCCTGA GTACAGTGAC GATGATGACC TGGAGCTTCA GGGTGCTGCA 
GCAGCCCCTA TCCCAAACTT TTTCTCCGAT GATGACTGCC TTGAAGACCT TCCTGAGAAG 
TTCGATGGCA ACCCTGACAT GCTGGGTCCT TTCATGTATC AGTGCCAGCT CTTCATGGAA 
AAGAGCACCA GAGATTTCTC AGTTGACCGC ATCCGTGTGT GCTTCGTGAC AAGCATGCTG 
ATCGGCCGTG CCGCCCGCTG GGCTACTGCC AAGCTGCAAA GATGTACTTA CCTGATGCAC 
AACTACACTG CCTTTATGAT GGAGCTGAAG CATGTCTTTG AAGACCCTCA GAGACGTGAA 
GCTGCCAAAC GCAAGATCAG ACGTCTGCGC CAGGGCCCTG GGCCTGTTGT GGACTACTCC 
AATGCATTCC AGATGATTGC CCAGGACCTG GATTGGACTG AGCCTGCCCT GATGGATCAG 
TTCCAGGAAG GTCTCAACCC AGACATTCGC GCAGAGCTGT CTCGCCAGGA GGCCCCCAAG 
ACCCTGGCTG CTCTGATTAC TGCCTGTATT CACATCGAGA GAAGGCTGGC TCGTGACGCT 
GCTGCAAAGC CCGATCCTTC ACCCAGAGCC TTGGTGATGC CTCCAAACAG CCAGACCGAT 
CCCACCGAGC CTGTGGGAGG TGCCCGCATG CGCCTGTCCA AGGAAGAAAA GGAGAGACGC 
CGCAAAATGA ATTTGTGTCT CTACTGTGGC AATGGAGGCC ATTTCGCCGA CACGTGTCCA 
GCGAAAGCCT CCAAGAATTC GCCGCCGGGA AACTCCCCGG CCCCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Peg10 ( NM_130877.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_570947.2 Mus musculus paternally expressed 10 (Peg10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu83568
Clone ID Related Accession (Same CDS sequence)	NM_130877.2
Accession Version	NM_130877.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	retrotransposon-derived protein PEG10 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ127881.1, AB091827.1, AC084315.4, CF735227.1 and BC094558.1. On May 18, 2006 this sequence version replaced NM_130877.1. Transcript Variant: This variant (1) has two overlapping open reading frames (RFI and RF2). This longer isoform (1) is derived from RF1/RF2 by -1 translational frameshifting. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CJ128728.1, BU519162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 12840045 ribosomal slippage :: PMID: 11574691, 17942406 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCTG CGGGTGGTTC CTCCAACTGC CCGCCCCCTC CCCCTCCCCC TCCTCCCAAC 
AACAACAACA ACAACAACAC CCCAAAGAGC CCAGGCGTGC CTGACGCCGA AGATGATGAT 
GAACGCAGAC ACGATGAGCT CCCTGAAGAC ATCAACAACT TTGACGAAGA CATGAACAGG 
CAGTTTGAGA ATATGAACCT GCTGGATCAG GTGGAGTTGC TTGCACAGAG CTACAGTCTG 
CTGGATCATT TAGATGACTT TGATGATGAT GATGAAGACG ATGACTTTGA TCCAGAACCT 
GACCAGGATG AGCTCCCTGA GTACAGTGAC GATGATGACC TGGAGCTTCA GGGTGCTGCA 
GCAGCCCCTA TCCCAAACTT TTTCTCCGAT GATGACTGCC TTGAAGACCT TCCTGAGAAG 
TTCGATGGCA ACCCTGACAT GCTGGGTCCT TTCATGTATC AGTGCCAGCT CTTCATGGAA 
AAGAGCACCA GAGATTTCTC AGTTGACCGC ATCCGTGTGT GCTTCGTGAC AAGCATGCTG 
ATCGGCCGTG CCGCCCGCTG GGCTACTGCC AAGCTGCAAA GATGTACTTA CCTGATGCAC 
AACTACACTG CCTTTATGAT GGAGCTGAAG CATGTCTTTG AAGACCCTCA GAGACGTGAA 
GCTGCCAAAC GCAAGATCAG ACGTCTGCGC CAGGGCCCTG GGCCTGTTGT GGACTACTCC 
AATGCATTCC AGATGATTGC CCAGGACCTG GATTGGACTG AGCCTGCCCT GATGGATCAG 
TTCCAGGAAG GTCTCAACCC AGACATTCGC GCAGAGCTGT CTCGCCAGGA GGCCCCCAAG 
ACCCTGGCTG CTCTGATTAC TGCCTGTATT CACATCGAGA GAAGGCTGGC TCGTGACGCT 
GCTGCAAAGC CCGATCCTTC ACCCAGAGCC TTGGTGATGC CTCCAAACAG CCAGACCGAT 
CCCACCGAGC CTGTGGGAGG TGCCCGCATG CGCCTGTCCA AGGAAGAAAA GGAGAGACGC 
CGCAAAATGA ATTTGTGTCT CTACTGTGGC AATGGAGGCC ATTTCGCCGA CACGTGTCCA 
GCGAAAGCCT CCAAGAATTC GCCGCCGGGA AAACTCCCCG GCCCCGCTGT AGGGGGACCT 
TCAGCGACAG GGCCAGAACG AATAAGGTCC CCACCCTCCG AGGCTTCGAC TCAGCACCTG 
CAAGTGATGC TCCAGATTCA TATGCCGGGC AGACCCACCC TGTTTGTCCG AGCTATGATT 
GATTCTGGTG CATCTGGCAA CTTCATTGAT CAAGACTTTG TCATACAAAA TGCAATTCCT 
CTCAGAATCA AAGACTGGCC AGTGATGGTG GAAGCTATTG ATGGGCATCC AATTGCCTCG 
GGCCCAATCA TTTTGGAAAC CCACCACCTG ATAGTTGATC TGGGAGACCA CCGTGAGATA 
CTGTCATTTG ATGTGACTCA GTCTCCATTC TTTCCTATTG TCCTAGGAAT TCGTTGGCTG 
AGCACGCATG ACCCTCACAT TACCTGGAGT ACCCGCTCCA TTGTCTTCAA CTCTGATTAC 
TGCCGACTTC GCTGCCGGAT GTTTGCACAG ATACCTTCTA ACTTACTGTT TACAGTGCCA 
CAACCGAATT TGCATCCGTA TCTACTTCAT CATGTGCATC CGCATGTCCA TCCGCATATG 
CATCAGCATC TGCATCAGCA TCTGCATCAG TTTCTGCATC CAGATCCGCA TCAGTATCCG 
CATCCGGATC CGCATTATCA TCATCATCAG CAGGCGGATA TGCAGCACCA ACTGCAGCAG 
TATCTATATC AGTATTTGTA TTACCATCTG TATCCGGTTA TGCACCACCA TCTGCCTCCA 
GATCAGCATG AGCATCTGCA TGAGTATCTG CATCAGTATC TGCATCAGTA TCTGCATCAG 
TTTCTGCATC ACCATCTGCA TCCGGATCTG CATCAGTATC TGTATCAGTA TCTGCATAAC 
CATATGAATC CGGATCCACA TCACCATCCT CATCCAGATC CCCCTCAGGA TCCACATCAC 
CCTCCACATC AGGATCCACA TCAGCATCCG GATCCCCATC AGGATCCTCC ACATCAGGAT 
CCACATCAGC ATCCGGATCC CCATCAGGAT CCTCCACATC AGGATCCACA TCAGCATCCG 
GATCCCCATC AGGATCCTCC ACATCAGGAT CCACATCAGG ATGCACATCA GCATCAGGAT 
CCCCATCAGG ATGCACATCA GGATCCACAT CAGGATGCAC ATCAGGATCC CCATATGGAT 
CCACACCTGC ATCAGCACCA GCATCCGCAG CCGCAGCCGC ATCCACAACA GCATCCTAAC 
CATCCTCAGC AGCCACCATT CTTCTACCAC ATGGCTGGAT TCAGAATTTA CCACCCTGTA 
AGGTATTACT ATATTCAGAA TGTGTATACA CCTGTTGATG AGCATGTCTA TCCGGGTCAC 
CGGGTGGTTG ACCCTAACAT TGAGATGATT CCTGGAGCGC ACAGCCTGCC CAGTGGACAT 
TTGTACTCAA TGTCTGAGTC TGAAATGAAT GCTCTGCGAA ATTTCGTGGA CAGGAATGTT 
AAAGATGGGC TCATGACTCC CACTGTGGCG CCCAATGGAG CCCAAGTCCT GCAAGTGAAA 
AGAGGGTGGA AACTCCAAGT CACTTACAAT TGCCGAGCTC CACAGAGTGG CACCATCCAA 
AATCAGTACC TACGCATGTC TCTTCCAAAT ATGGGAGACC CTGCACACCT GGCAAGCTAT 
GGTGAATTTG TCCAAGTTCC TGGCTACCCA TATCCAGCCT ATGTTTACTA TACAAGCCCG 
CATATGATGA CTGCGTGGTA CCCAGTAGGA CGAGATGTAC ATGGACGAAT AATCGTTGTG 
CCTGTTGTAA TCACCTGGTC TCAAAATACG AACCGCCAGC CTCCGGTGCC CCAGTATCCT 
CCTCCGCAGC CACCTCCACC ACCACCACCA CCTCCACCGC CACCACCACC TCCACCAGCA 
TCATCCTGCA GTGCTGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570947.2
CDS	join(371..1480,1480..3390)
Translation	MAAAGGSSNCPPPPPPPPPNNNNNNNTPKSPGVPDAEDDDERRHDELPEDINNFDEDMNRQFENMNLLDQVELLAQSYSLLDHLDDFDDDDEDDDFDPEPDQDELPEYSDDDDLELQGAAAAPIPNFFSDDDCLEDLPEKFDGNPDMLGPFMYQCQLFMEKSTRDFSVDRIRVCFVTSMLIGRAARWATAKLQRCTYLMHNYTAFMMELKHVFEDPQRREAAKRKIRRLRQGPGPVVDYSNAFQMIAQDLDWTEPALMDQFQEGLNPDIRAELSRQEAPKTLAALITACIHIERRLARDAAAKPDPSPRALVMPPNSQTDPTEPVGGARMRLSKEEKERRRKMNLCLYCGNGGHFADTCPAKASKNSPPGKLPGPAVGGPSATGPERIRSPPSEASTQHLQVMLQIHMPGRPTLFVRAMIDSGASGNFIDQDFVIQNAIPLRIKDWPVMVEAIDGHPIASGPIILETHHLIVDLGDHREILSFDVTQSPFFPIVLGIRWLSTHDPHITWSTRSIVFNSDYCRLRCRMFAQIPSNLLFTVPQPNLHPYLLHHVHPHVHPHMHQHLHQHLHQFLHPDPHQYPHPDPHYHHHQQADMQHQLQQYLYQYLYYHLYPVMHHHLPPDQHEHLHEYLHQYLHQYLHQFLHHHLHPDLHQYLYQYLHNHMNPDPHHHPHPDPPQDPHHPPHQDPHQHPDPHQDPPHQDPHQHPDPHQDPPHQDPHQHPDPHQDPPHQDPHQDAHQHQDPHQDAHQDPHQDAHQDPHMDPHLHQHQHPQPQPHPQQHPNHPQQPPFFYHMAGFRIYHPVRYYYIQNVYTPVDEHVYPGHRVVDPNIEMIPGAHSLPSGHLYSMSESEMNALRNFVDRNVKDGLMTPTVAPNGAQVLQVKRGWKLQVTYNCRAPQSGTIQNQYLRMSLPNMGDPAHLASYGEFVQVPGYPYPAYVYYTSPHMMTAWYPVGRDVHGRIIVVPVVITWSQNTNRQPPVPQYPPPQPPPPPPPPPPPPPPPPASSCSAA

Target ORF information:

RefSeq Version	NM_130877.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus paternally expressed 10 (Peg10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130877.2

ORF Insert Sequence:

ATGGCTGCTG CGGGTGGTTC CTCCAACTGC CCGCCCCCTC CCCCTCCCCC TCCTCCCAAC 
AACAACAACA ACAACAACAC CCCAAAGAGC CCAGGCGTGC CTGACGCCGA AGATGATGAT 
GAACGCAGAC ACGATGAGCT CCCTGAAGAC ATCAACAACT TTGACGAAGA CATGAACAGG 
CAGTTTGAGA ATATGAACCT GCTGGATCAG GTGGAGTTGC TTGCACAGAG CTACAGTCTG 
CTGGATCATT TAGATGACTT TGATGATGAT GATGAAGACG ATGACTTTGA TCCAGAACCT 
GACCAGGATG AGCTCCCTGA GTACAGTGAC GATGATGACC TGGAGCTTCA GGGTGCTGCA 
GCAGCCCCTA TCCCAAACTT TTTCTCCGAT GATGACTGCC TTGAAGACCT TCCTGAGAAG 
TTCGATGGCA ACCCTGACAT GCTGGGTCCT TTCATGTATC AGTGCCAGCT CTTCATGGAA 
AAGAGCACCA GAGATTTCTC AGTTGACCGC ATCCGTGTGT GCTTCGTGAC AAGCATGCTG 
ATCGGCCGTG CCGCCCGCTG GGCTACTGCC AAGCTGCAAA GATGTACTTA CCTGATGCAC 
AACTACACTG CCTTTATGAT GGAGCTGAAG CATGTCTTTG AAGACCCTCA GAGACGTGAA 
GCTGCCAAAC GCAAGATCAG ACGTCTGCGC CAGGGCCCTG GGCCTGTTGT GGACTACTCC 
AATGCATTCC AGATGATTGC CCAGGACCTG GATTGGACTG AGCCTGCCCT GATGGATCAG 
TTCCAGGAAG GTCTCAACCC AGACATTCGC GCAGAGCTGT CTCGCCAGGA GGCCCCCAAG 
ACCCTGGCTG CTCTGATTAC TGCCTGTATT CACATCGAGA GAAGGCTGGC TCGTGACGCT 
GCTGCAAAGC CCGATCCTTC ACCCAGAGCC TTGGTGATGC CTCCAAACAG CCAGACCGAT 
CCCACCGAGC CTGTGGGAGG TGCCCGCATG CGCCTGTCCA AGGAAGAAAA GGAGAGACGC 
CGCAAAATGA ATTTGTGTCT CTACTGTGGC AATGGAGGCC ATTTCGCCGA CACGTGTCCA 
GCGAAAGCCT CCAAGAATTC GCCGCCGGGA AAACTCCCCG GCCCCGCTGT AGGGGGACCT 
TCAGCGACAG GGCCAGAACG AATAAGGTCC CCACCCTCCG AGGCTTCGAC TCAGCACCTG 
CAAGTGATGC TCCAGATTCA TATGCCGGGC AGACCCACCC TGTTTGTCCG AGCTATGATT 
GATTCTGGTG CATCTGGCAA CTTCATTGAT CAAGACTTTG TCATACAAAA TGCAATTCCT 
CTCAGAATCA AAGACTGGCC AGTGATGGTG GAAGCTATTG ATGGGCATCC AATTGCCTCG 
GGCCCAATCA TTTTGGAAAC CCACCACCTG ATAGTTGATC TGGGAGACCA CCGTGAGATA 
CTGTCATTTG ATGTGACTCA GTCTCCATTC TTTCCTATTG TCCTAGGAAT TCGTTGGCTG 
AGCACGCATG ACCCTCACAT TACCTGGAGT ACCCGCTCCA TTGTCTTCAA CTCTGATTAC 
TGCCGACTTC GCTGCCGGAT GTTTGCACAG ATACCTTCTA ACTTACTGTT TACAGTGCCA 
CAACCGAATT TGCATCCGTA TCTACTTCAT CATGTGCATC CGCATGTCCA TCCGCATATG 
CATCAGCATC TGCATCAGCA TCTGCATCAG TTTCTGCATC CAGATCCGCA TCAGTATCCG 
CATCCGGATC CGCATTATCA TCATCATCAG CAGGCGGATA TGCAGCACCA ACTGCAGCAG 
TATCTATATC AGTATTTGTA TTACCATCTG TATCCGGTTA TGCACCACCA TCTGCCTCCA 
GATCAGCATG AGCATCTGCA TGAGTATCTG CATCAGTATC TGCATCAGTA TCTGCATCAG 
TTTCTGCATC ACCATCTGCA TCCGGATCTG CATCAGTATC TGTATCAGTA TCTGCATAAC 
CATATGAATC CGGATCCACA TCACCATCCT CATCCAGATC CCCCTCAGGA TCCACATCAC 
CCTCCACATC AGGATCCACA TCAGCATCCG GATCCCCATC AGGATCCTCC ACATCAGGAT 
CCACATCAGC ATCCGGATCC CCATCAGGAT CCTCCACATC AGGATCCACA TCAGCATCCG 
GATCCCCATC AGGATCCTCC ACATCAGGAT CCACATCAGG ATGCACATCA GCATCAGGAT 
CCCCATCAGG ATGCACATCA GGATCCACAT CAGGATGCAC ATCAGGATCC CCATATGGAT 
CCACACCTGC ATCAGCACCA GCATCCGCAG CCGCAGCCGC ATCCACAACA GCATCCTAAC 
CATCCTCAGC AGCCACCATT CTTCTACCAC ATGGCTGGAT TCAGAATTTA CCACCCTGTA 
AGGTATTACT ATATTCAGAA TGTGTATACA CCTGTTGATG AGCATGTCTA TCCGGGTCAC 
CGGGTGGTTG ACCCTAACAT TGAGATGATT CCTGGAGCGC ACAGCCTGCC CAGTGGACAT 
TTGTACTCAA TGTCTGAGTC TGAAATGAAT GCTCTGCGAA ATTTCGTGGA CAGGAATGTT 
AAAGATGGGC TCATGACTCC CACTGTGGCG CCCAATGGAG CCCAAGTCCT GCAAGTGAAA 
AGAGGGTGGA AACTCCAAGT CACTTACAAT TGCCGAGCTC CACAGAGTGG CACCATCCAA 
AATCAGTACC TACGCATGTC TCTTCCAAAT ATGGGAGACC CTGCACACCT GGCAAGCTAT 
GGTGAATTTG TCCAAGTTCC TGGCTACCCA TATCCAGCCT ATGTTTACTA TACAAGCCCG 
CATATGATGA CTGCGTGGTA CCCAGTAGGA CGAGATGTAC ATGGACGAAT AATCGTTGTG 
CCTGTTGTAA TCACCTGGTC TCAAAATACG AACCGCCAGC CTCCGGTGCC CCAGTATCCT 
CCTCCGCAGC CACCTCCACC ACCACCACCA CCTCCACCGC CACCACCACC TCCACCAGCA 
TCATCCTGCA GTGCTGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mammalian gene PEG10 expresses two reading frames by high efficiency -1 frameshifting in embryonic-associated tissues.
The Journal of biological chemistry282(52)37359-69(2007 Dec)
Clark MB,J?nicke M,Gottesb?hren U,Kleffmann T,Legge M,Poole ES,Tate WP

Deletion of Peg10, an imprinted gene acquired from a retrotransposon, causes early embryonic lethality.
Nature genetics38(1)101-6(2006 Jan)
Ono R,Nakamura K,Inoue K,Naruse M,Usami T,Wakisaka-Saito N,Hino T,Suzuki-Migishima R,Ogonuki N,Miki H,Kohda T,Ogura A,Yokoyama M,Kaneko-Ishino T,Ishino F

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Peg10 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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