PEG10 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PEG10
Entrez Gene ID	618138
Full Name	paternally expressed 10
General protein information	Preferred Names paternally expressed 10 Names retrotransposon-derived protein PEG10
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	This is a paternally expressed imprinted gene that is thought to have been derived from the Ty3/Gypsy family of retrotransposons. It contains two overlapping open reading frames, RF1 and RF2, and expresses two proteins: a shorter, gag-like protein (with a CCHC-type zinc finger domain) from RF1; and a longer, gag/pol-like fusion protein (with an additional aspartic protease motif) from RF1/RF2 by -1 translational frameshifting (-1 FS). While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements required for -1 FS. It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation, apoptosis and cancer development. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. Different isoforms resulting from alternatively spliced transcript variants, and use of non-AUG (CUG) translation initiation codon have been found for this gene. [provided by RefSeq, Oct 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001172519.2	NP_001165990.2	retrotransposon-derived protein PEG10 isoform 2
NM_001172518.2	NP_001165989.2	retrotransposon-derived protein PEG10 isoform 1
NM_001109815.5	NP_001103285.1	retrotransposon-derived protein PEG10 isoform 4
NM_001127210.3	NP_001120682.1	retrotransposon-derived protein PEG10 isoform 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Peg10	NP_570947.2
Canis lupus familiaris (dog)	PEG10	NP_001166014.2
Homo sapiens (human)	PEG10	NP_001165908.1
Pan troglodytes (chimpanzee)	PEG10	NP_001165897.1
	PEG10	NP_001165893.1
Rattus norvegicus (Norway rat)	LOC102553587	XP_006236145.1
Bos taurus (cattle)	PEG10	NP_001120682.1

Gene Ontology
Bibliography

Related articles in PubMed

[The methylation status of PEG10 in placentas of cloned transgenic calves].
Su JM, Xu WB, Li YY, Wang LJ, Wang YS, Zhang Y
Yi chuan = Hereditas33(5)533-8(2011 May)

Genetic and molecular analyses of PEG10 reveal new aspects of genomic organization, transcription and translation.
Lux H, Flammann H, Hafner M, Lux A
PloS one5(1)e8686(2010 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Mammalian gene PEG10 expresses two reading frames by high efficiency -1 frameshifting in embryonic-associated tissues.
Clark MB, J?nicke M, Gottesb?hren U, Kleffmann T, Legge M, Poole ES, Tate WP
The Journal of biological chemistry282(52)37359-69(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the abnormal DNA methylation epigenetic reprogramming of imprinting PEG10 gene in placenta may be one of the main causes of the abnormal development and death of the transgenic cloned cattle.
Title: [The methylation status of PEG10 in placentas of cloned transgenic calves].

The following PEG10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PEG10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa134026	NM_001127210.3 Latest version!	Bos taurus paternally expressed 10 (PEG10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa134033	NM_001172518.2 Latest version!	Bos taurus paternally expressed 10 (PEG10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa40028	NM_001109815.5 Latest version!	Bos taurus paternally expressed 10 (PEG10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OBa56515	NM_001172519.2 Latest version!	Bos taurus paternally expressed 10 (PEG10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PEG10 ( NM_001127210.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001120682.1 Bos taurus paternally expressed 10 (PEG10), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OBa134026
Clone ID Related Accession (Same CDS sequence)	NM_001127210.3
Accession Version	NM_001127210.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-13
Organism	Bos taurus(cattle)
Product	retrotransposon-derived protein PEG10 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV621212.1, EV620062.1, NKLS02000004.1, EE376683.1, EE391829.1, EE371339.1, EE381872.1, DV928493.1, EE376643.1, EE381933.1, CB450408.1 and EH200262.1. On Jun 14, 2012 this sequence version replaced NM_001127210.2. Transcript Variant: This variant (2) has two overlapping open reading frames (RF1 and RF2). It uses an alternate donor splice site at the 5' terminal exon and initiates translation from an alternate, upstream AUG compared to variant 1. This isoform (3) derived from RF1/RF2 by -1 translational frameshifting has a longer and distinct N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.46034.1, SRR5571258.1881.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145420 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 21586401 ribosomal slippage :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGAAACA AAAAGCTTCT GAAAACCAGA AGGCGAAAAG GTGGACGCGG AGGCCAGGAC 
ACAGGCCTCC ATTGCCACAG GAGTGAAGCT ACTACGCGCG GGAGGTCTCC TCCCACCCCA 
ACCATCACCC TGGGGCCCGA CTGCCCACCA CCTCCGCCTC CTCCTCCCCC CAACGATCCT 
TCCTCCCCCT CCAACTCCCC CTGCAGCAGC CAGAGGGGCC AGTACAACCC AAACCTGGGA 
GAACGCCGGC GGGAGGATCT CTCAGAGGAG ATCAACAACC TCAGAGAGAA GGTCATGAAG 
CAGTCCGAGG AGAACAACAA CCTGCAGAAC CAGGTGCAGA AACTCACCGA GGAGAACACC 
ACCCTCCGGG AGCAAGTGGA GCCCACCCCC GAGGAGGAAG AGGACGACAT TGAGCTGTGC 
GGTGCAGCGG CCGCCGCCGC CCCAGCCTCC CCTATCGAGG AGGAGTGCCC AGAAGACCTT 
CCCGAGAAGT TCGACGGCAA CCCAGACATG CTGGTTCCCT TCATGTCCCA GTGCCAGCTC 
TTCATGGAGA AGAGCACCCG CGATTTCTCT GTCGATCGCG TGCGCGTCTG CTTCGTGACC 
AGCATGATGA CGGGCCGCGC TGCCCGCTGG GCCTCGGCCA AACTGGAGCG TTCTCACTAT 
CTCATGCACA ACTACCCAGC CTTCATGACC GAAATGAAGC ACGTCTTTGA AGATCCCCAG 
CGGCGCGAGG CTGCCAAACG CAAGATTAGA CGTCTGCGCC AGGGCATGGG GTCAGTGGTC 
GACTACTCTA ATGCTTTCCA GATGATCGCG CAGGACCTGG ATTGGAACGA GCCCGCCCTG 
ATCGACCAGT ACCACGAGGG CCTCAGCGAC CACATCCAGG CAGAGCTGGC GCGCCTCGAA 
GTGGCCAAGT CGCTGTCCGC GCTCATCGGC CAGTGCATCC ACATTGAGAG AAGGCTGGCC 
CGGGCTGCCG CCACCCGCAA GCCGCGCTCC CCGCCACGCG CGCTGGTGGC GCCGCACGTC 
AACAATCACC ACAACCACCA GGTAGACCCG ACTGAGCCCG TGGGGGGCGC CCGCATGCGC 
CTGACGCAGG AAGAGAAAGA GAGACGTCGC AAGCTGAACC TGTGCCTCTA TTGTGGGAAT 
GGAGGCCACT ACGCTGACAA CTGTCCTGCC AAGGCCTCGA AGGCTTCGCC GGCGGGAAAA 
CTCCCCGGCC CCGCTGTAGA GGGACCTTCA GCGACCGGGC CGGAATTAAT AAGGTCCCCC 
CAAGATGATG CTGCGTCATC TCCACACTTG CAAGTGATGC TCCAGATTCA TCTCCCGGGC 
CGACACAGCC TGTTCGTCCG AGCCATGATC GATTCTGGCG CTTCTGGCAA TTTCATTGAT 
CACGAGTACG TGGCCCAGAA CGGCATTCCT CTGAGAATCA AGGACTGGCC CATACTTGTA 
GAAGCAATTG ATGGACGTCC CATAGCATCG GGCCCCGTGG TCCACGAAAC ACATGACCTG 
ATCGTCGACT TGGGAGATCA CCGCGAGGTG CTGTCGTTCG ATGTGACTCA GTCTCCGTTC 
TTCCCCATCG TCCTGGGGGT GCGCTGGCTG AGCACACACG ATCCCAACAT CACATGGAGC 
ACCCGATCGA TCGTCTTCGA TTCCGAATAT TGCAGATACC ACTGCCGGAT GTATTCTCCG 
ATACCACCAC CTCTCCCGCC ACCAGCACAA CCGGCGTTCT ATTACCCGGT AGAGGGGTAC 
AGAGTTTACC AGCCTGTGAG ATACTACTAC GTGCAGAATG TGTACACCCC AGTGGATGAG 
AACGTCTACC CAGACCACCG TCTGGTGGAC CCTAACATAG AAATGATCCC CGGAGCTCAC 
AGTATTCCCA GCGGGCATGT GTACTCACTG TCGGAATCTG AAATGGCAGC CCTGCGAGAT 
TTCGTCGCCA GACATGTGAA GGATGGGCTG ATCACTCCCA CCATCGCGCC AAACGGAGCC 
CAAGTTCTCC AAGTGAAAAG AGGGTGGAAG CTGCAAGTTT CTTACGACTG CCGGGGTCCT 
AACGGTGTCA CCATCCAGAA TCAGTATCCT CGACTCACAA TTCCAAATTT AGATGACCAA 
GCTCACCTGG CGACGTACAC TGAATACGTA CCTCAAATTC CAGGATATCC GACCTACCCC 
GCCTATGCGT ACCCGACCTA CCCAGTAGGA TTCGCCTGGT ACCCCGTGGG GCGAGATGGA 
CACGGGCGAT CCCTCTATGT CCCCGTGATG ATCACCTGGA ATCCTCATTG GTACCGCCAG 
CCGCCAGTGC CCCAGTACCC GCCGCCGCAG CCTCCACCGC CGCCACCCCC GCCGCCACCC 
CCGCCTCCGT CTTACAGCGC CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120682.1
CDS	join(218..1414,1414..2583)
Translation	MRNKKLLKTRRRKGGRGGQDTGLHCHRSEATTRGRSPPTPTITLGPDCPPPPPPPPPNDPSSPSNSPCSSQRGQYNPNLGERRREDLSEEINNLREKVMKQSEENNNLQNQVQKLTEENTTLREQVEPTPEEEEDDIELCGAAAAAAPASPIEEECPEDLPEKFDGNPDMLVPFMSQCQLFMEKSTRDFSVDRVRVCFVTSMMTGRAARWASAKLERSHYLMHNYPAFMTEMKHVFEDPQRREAAKRKIRRLRQGMGSVVDYSNAFQMIAQDLDWNEPALIDQYHEGLSDHIQAELARLEVAKSLSALIGQCIHIERRLARAAATRKPRSPPRALVAPHVNNHHNHQVDPTEPVGGARMRLTQEEKERRRKLNLCLYCGNGGHYADNCPAKASKASPAGKLPGPAVEGPSATGPELIRSPQDDAASSPHLQVMLQIHLPGRHSLFVRAMIDSGASGNFIDHEYVAQNGIPLRIKDWPILVEAIDGRPIASGPVVHETHDLIVDLGDHREVLSFDVTQSPFFPIVLGVRWLSTHDPNITWSTRSIVFDSEYCRYHCRMYSPIPPPLPPPAQPAFYYPVEGYRVYQPVRYYYVQNVYTPVDENVYPDHRLVDPNIEMIPGAHSIPSGHVYSLSESEMAALRDFVARHVKDGLITPTIAPNGAQVLQVKRGWKLQVSYDCRGPNGVTIQNQYPRLTIPNLDDQAHLATYTEYVPQIPGYPTYPAYAYPTYPVGFAWYPVGRDGHGRSLYVPVMITWNPHWYRQPPVPQYPPPQPPPPPPPPPPPPPSYSAL

Target ORF information:

RefSeq Version	NM_001127210.3
Organism	Bos taurus(cattle)
Definition	Bos taurus paternally expressed 10 (PEG10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127210.3

ORF Insert Sequence:

ATGCGAAACA AAAAGCTTCT GAAAACCAGA AGGCGAAAAG GTGGACGCGG AGGCCAGGAC 
ACAGGCCTCC ATTGCCACAG GAGTGAAGCT ACTACGCGCG GGAGGTCTCC TCCCACCCCA 
ACCATCACCC TGGGGCCCGA CTGCCCACCA CCTCCGCCTC CTCCTCCCCC CAACGATCCT 
TCCTCCCCCT CCAACTCCCC CTGCAGCAGC CAGAGGGGCC AGTACAACCC AAACCTGGGA 
GAACGCCGGC GGGAGGATCT CTCAGAGGAG ATCAACAACC TCAGAGAGAA GGTCATGAAG 
CAGTCCGAGG AGAACAACAA CCTGCAGAAC CAGGTGCAGA AACTCACCGA GGAGAACACC 
ACCCTCCGGG AGCAAGTGGA GCCCACCCCC GAGGAGGAAG AGGACGACAT TGAGCTGTGC 
GGTGCAGCGG CCGCCGCCGC CCCAGCCTCC CCTATCGAGG AGGAGTGCCC AGAAGACCTT 
CCCGAGAAGT TCGACGGCAA CCCAGACATG CTGGTTCCCT TCATGTCCCA GTGCCAGCTC 
TTCATGGAGA AGAGCACCCG CGATTTCTCT GTCGATCGCG TGCGCGTCTG CTTCGTGACC 
AGCATGATGA CGGGCCGCGC TGCCCGCTGG GCCTCGGCCA AACTGGAGCG TTCTCACTAT 
CTCATGCACA ACTACCCAGC CTTCATGACC GAAATGAAGC ACGTCTTTGA AGATCCCCAG 
CGGCGCGAGG CTGCCAAACG CAAGATTAGA CGTCTGCGCC AGGGCATGGG GTCAGTGGTC 
GACTACTCTA ATGCTTTCCA GATGATCGCG CAGGACCTGG ATTGGAACGA GCCCGCCCTG 
ATCGACCAGT ACCACGAGGG CCTCAGCGAC CACATCCAGG CAGAGCTGGC GCGCCTCGAA 
GTGGCCAAGT CGCTGTCCGC GCTCATCGGC CAGTGCATCC ACATTGAGAG AAGGCTGGCC 
CGGGCTGCCG CCACCCGCAA GCCGCGCTCC CCGCCACGCG CGCTGGTGGC GCCGCACGTC 
AACAATCACC ACAACCACCA GGTAGACCCG ACTGAGCCCG TGGGGGGCGC CCGCATGCGC 
CTGACGCAGG AAGAGAAAGA GAGACGTCGC AAGCTGAACC TGTGCCTCTA TTGTGGGAAT 
GGAGGCCACT ACGCTGACAA CTGTCCTGCC AAGGCCTCGA AGGCTTCGCC GGCGGGAAAA 
CTCCCCGGCC CCGCTGTAGA GGGACCTTCA GCGACCGGGC CGGAATTAAT AAGGTCCCCC 
CAAGATGATG CTGCGTCATC TCCACACTTG CAAGTGATGC TCCAGATTCA TCTCCCGGGC 
CGACACAGCC TGTTCGTCCG AGCCATGATC GATTCTGGCG CTTCTGGCAA TTTCATTGAT 
CACGAGTACG TGGCCCAGAA CGGCATTCCT CTGAGAATCA AGGACTGGCC CATACTTGTA 
GAAGCAATTG ATGGACGTCC CATAGCATCG GGCCCCGTGG TCCACGAAAC ACATGACCTG 
ATCGTCGACT TGGGAGATCA CCGCGAGGTG CTGTCGTTCG ATGTGACTCA GTCTCCGTTC 
TTCCCCATCG TCCTGGGGGT GCGCTGGCTG AGCACACACG ATCCCAACAT CACATGGAGC 
ACCCGATCGA TCGTCTTCGA TTCCGAATAT TGCAGATACC ACTGCCGGAT GTATTCTCCG 
ATACCACCAC CTCTCCCGCC ACCAGCACAA CCGGCGTTCT ATTACCCGGT AGAGGGGTAC 
AGAGTTTACC AGCCTGTGAG ATACTACTAC GTGCAGAATG TGTACACCCC AGTGGATGAG 
AACGTCTACC CAGACCACCG TCTGGTGGAC CCTAACATAG AAATGATCCC CGGAGCTCAC 
AGTATTCCCA GCGGGCATGT GTACTCACTG TCGGAATCTG AAATGGCAGC CCTGCGAGAT 
TTCGTCGCCA GACATGTGAA GGATGGGCTG ATCACTCCCA CCATCGCGCC AAACGGAGCC 
CAAGTTCTCC AAGTGAAAAG AGGGTGGAAG CTGCAAGTTT CTTACGACTG CCGGGGTCCT 
AACGGTGTCA CCATCCAGAA TCAGTATCCT CGACTCACAA TTCCAAATTT AGATGACCAA 
GCTCACCTGG CGACGTACAC TGAATACGTA CCTCAAATTC CAGGATATCC GACCTACCCC 
GCCTATGCGT ACCCGACCTA CCCAGTAGGA TTCGCCTGGT ACCCCGTGGG GCGAGATGGA 
CACGGGCGAT CCCTCTATGT CCCCGTGATG ATCACCTGGA ATCCTCATTG GTACCGCCAG 
CCGCCAGTGC CCCAGTACCC GCCGCCGCAG CCTCCACCGC CGCCACCCCC GCCGCCACCC 
CCGCCTCCGT CTTACAGCGC CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEG10 ( NM_001172518.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001165989.2 Bos taurus paternally expressed 10 (PEG10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa134033
Clone ID Related Accession (Same CDS sequence)	NM_001172518.2
Accession Version	NM_001172518.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-13
Organism	Bos taurus(cattle)
Product	retrotransposon-derived protein PEG10 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV615229.1, EV620062.1, NKLS02000004.1, EE376683.1, EE391829.1, EE371339.1, EE381872.1, DV928493.1, EE376643.1, EE381933.1, CB450408.1 and EH200262.1. On Jun 14, 2012 this sequence version replaced NM_001172518.1. Transcript Variant: This variant (1) has two overlapping open reading frames (RF1 and RF2) and initiates translation from a non-AUG (CUG) start codon. This isoform (1) is derived from RF1/RF2 by -1 translational frameshifting. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.46010.1, SRR5571257.56332.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145420 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 21586401 non-AUG initiation codon :: inferred from conservation ribosomal slippage :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGGGCCCG ACTGCCCACC ACCTCCGCCT CCTCCTCCCC CCAACGATCC TTCCTCCCCC 
TCCAACTCCC CCTGCAGCAG CCAGAGGGGC CAGTACAACC CAAACCTGGG AGAACGCCGG 
CGGGAGGATC TCTCAGAGGA GATCAACAAC CTCAGAGAGA AGGTCATGAA GCAGTCCGAG 
GAGAACAACA ACCTGCAGAA CCAGGTGCAG AAACTCACCG AGGAGAACAC CACCCTCCGG 
GAGCAAGTGG AGCCCACCCC CGAGGAGGAA GAGGACGACA TTGAGCTGTG CGGTGCAGCG 
GCCGCCGCCG CCCCAGCCTC CCCTATCGAG GAGGAGTGCC CAGAAGACCT TCCCGAGAAG 
TTCGACGGCA ACCCAGACAT GCTGGTTCCC TTCATGTCCC AGTGCCAGCT CTTCATGGAG 
AAGAGCACCC GCGATTTCTC TGTCGATCGC GTGCGCGTCT GCTTCGTGAC CAGCATGATG 
ACGGGCCGCG CTGCCCGCTG GGCCTCGGCC AAACTGGAGC GTTCTCACTA TCTCATGCAC 
AACTACCCAG CCTTCATGAC CGAAATGAAG CACGTCTTTG AAGATCCCCA GCGGCGCGAG 
GCTGCCAAAC GCAAGATTAG ACGTCTGCGC CAGGGCATGG GGTCAGTGGT CGACTACTCT 
AATGCTTTCC AGATGATCGC GCAGGACCTG GATTGGAACG AGCCCGCCCT GATCGACCAG 
TACCACGAGG GCCTCAGCGA CCACATCCAG GCAGAGCTGG CGCGCCTCGA AGTGGCCAAG 
TCGCTGTCCG CGCTCATCGG CCAGTGCATC CACATTGAGA GAAGGCTGGC CCGGGCTGCC 
GCCACCCGCA AGCCGCGCTC CCCGCCACGC GCGCTGGTGG CGCCGCACGT CAACAATCAC 
CACAACCACC AGGTAGACCC GACTGAGCCC GTGGGGGGCG CCCGCATGCG CCTGACGCAG 
GAAGAGAAAG AGAGACGTCG CAAGCTGAAC CTGTGCCTCT ATTGTGGGAA TGGAGGCCAC 
TACGCTGACA ACTGTCCTGC CAAGGCCTCG AAGGCTTCGC CGGCGGGAAA ACTCCCCGGC 
CCCGCTGTAG AGGGACCTTC AGCGACCGGG CCGGAATTAA TAAGGTCCCC CCAAGATGAT 
GCTGCGTCAT CTCCACACTT GCAAGTGATG CTCCAGATTC ATCTCCCGGG CCGACACAGC 
CTGTTCGTCC GAGCCATGAT CGATTCTGGC GCTTCTGGCA ATTTCATTGA TCACGAGTAC 
GTGGCCCAGA ACGGCATTCC TCTGAGAATC AAGGACTGGC CCATACTTGT AGAAGCAATT 
GATGGACGTC CCATAGCATC GGGCCCCGTG GTCCACGAAA CACATGACCT GATCGTCGAC 
TTGGGAGATC ACCGCGAGGT GCTGTCGTTC GATGTGACTC AGTCTCCGTT CTTCCCCATC 
GTCCTGGGGG TGCGCTGGCT GAGCACACAC GATCCCAACA TCACATGGAG CACCCGATCG 
ATCGTCTTCG ATTCCGAATA TTGCAGATAC CACTGCCGGA TGTATTCTCC GATACCACCA 
CCTCTCCCGC CACCAGCACA ACCGGCGTTC TATTACCCGG TAGAGGGGTA CAGAGTTTAC 
CAGCCTGTGA GATACTACTA CGTGCAGAAT GTGTACACCC CAGTGGATGA GAACGTCTAC 
CCAGACCACC GTCTGGTGGA CCCTAACATA GAAATGATCC CCGGAGCTCA CAGTATTCCC 
AGCGGGCATG TGTACTCACT GTCGGAATCT GAAATGGCAG CCCTGCGAGA TTTCGTCGCC 
AGACATGTGA AGGATGGGCT GATCACTCCC ACCATCGCGC CAAACGGAGC CCAAGTTCTC 
CAAGTGAAAA GAGGGTGGAA GCTGCAAGTT TCTTACGACT GCCGGGGTCC TAACGGTGTC 
ACCATCCAGA ATCAGTATCC TCGACTCACA ATTCCAAATT TAGATGACCA AGCTCACCTG 
GCGACGTACA CTGAATACGT ACCTCAAATT CCAGGATATC CGACCTACCC CGCCTATGCG 
TACCCGACCT ACCCAGTAGG ATTCGCCTGG TACCCCGTGG GGCGAGATGG ACACGGGCGA 
TCCCTCTATG TCCCCGTGAT GATCACCTGG AATCCTCATT GGTACCGCCA GCCGCCAGTG 
CCCCAGTACC CGCCGCCGCA GCCTCCACCG CCGCCACCCC CGCCGCCACC CCCGCCTCCG 
TCTTACAGCG CCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165989.2
CDS	join(336..1403,1403..2572)
Translation	LGPDCPPPPPPPPPNDPSSPSNSPCSSQRGQYNPNLGERRREDLSEEINNLREKVMKQSEENNNLQNQVQKLTEENTTLREQVEPTPEEEEDDIELCGAAAAAAPASPIEEECPEDLPEKFDGNPDMLVPFMSQCQLFMEKSTRDFSVDRVRVCFVTSMMTGRAARWASAKLERSHYLMHNYPAFMTEMKHVFEDPQRREAAKRKIRRLRQGMGSVVDYSNAFQMIAQDLDWNEPALIDQYHEGLSDHIQAELARLEVAKSLSALIGQCIHIERRLARAAATRKPRSPPRALVAPHVNNHHNHQVDPTEPVGGARMRLTQEEKERRRKLNLCLYCGNGGHYADNCPAKASKASPAGKLPGPAVEGPSATGPELIRSPQDDAASSPHLQVMLQIHLPGRHSLFVRAMIDSGASGNFIDHEYVAQNGIPLRIKDWPILVEAIDGRPIASGPVVHETHDLIVDLGDHREVLSFDVTQSPFFPIVLGVRWLSTHDPNITWSTRSIVFDSEYCRYHCRMYSPIPPPLPPPAQPAFYYPVEGYRVYQPVRYYYVQNVYTPVDENVYPDHRLVDPNIEMIPGAHSIPSGHVYSLSESEMAALRDFVARHVKDGLITPTIAPNGAQVLQVKRGWKLQVSYDCRGPNGVTIQNQYPRLTIPNLDDQAHLATYTEYVPQIPGYPTYPAYAYPTYPVGFAWYPVGRDGHGRSLYVPVMITWNPHWYRQPPVPQYPPPQPPPPPPPPPPPPPSYSAL

Target ORF information:

RefSeq Version	NM_001172518.2
Organism	Bos taurus(cattle)
Definition	Bos taurus paternally expressed 10 (PEG10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172518.2

ORF Insert Sequence:

CTGGGGCCCG ACTGCCCACC ACCTCCGCCT CCTCCTCCCC CCAACGATCC TTCCTCCCCC 
TCCAACTCCC CCTGCAGCAG CCAGAGGGGC CAGTACAACC CAAACCTGGG AGAACGCCGG 
CGGGAGGATC TCTCAGAGGA GATCAACAAC CTCAGAGAGA AGGTCATGAA GCAGTCCGAG 
GAGAACAACA ACCTGCAGAA CCAGGTGCAG AAACTCACCG AGGAGAACAC CACCCTCCGG 
GAGCAAGTGG AGCCCACCCC CGAGGAGGAA GAGGACGACA TTGAGCTGTG CGGTGCAGCG 
GCCGCCGCCG CCCCAGCCTC CCCTATCGAG GAGGAGTGCC CAGAAGACCT TCCCGAGAAG 
TTCGACGGCA ACCCAGACAT GCTGGTTCCC TTCATGTCCC AGTGCCAGCT CTTCATGGAG 
AAGAGCACCC GCGATTTCTC TGTCGATCGC GTGCGCGTCT GCTTCGTGAC CAGCATGATG 
ACGGGCCGCG CTGCCCGCTG GGCCTCGGCC AAACTGGAGC GTTCTCACTA TCTCATGCAC 
AACTACCCAG CCTTCATGAC CGAAATGAAG CACGTCTTTG AAGATCCCCA GCGGCGCGAG 
GCTGCCAAAC GCAAGATTAG ACGTCTGCGC CAGGGCATGG GGTCAGTGGT CGACTACTCT 
AATGCTTTCC AGATGATCGC GCAGGACCTG GATTGGAACG AGCCCGCCCT GATCGACCAG 
TACCACGAGG GCCTCAGCGA CCACATCCAG GCAGAGCTGG CGCGCCTCGA AGTGGCCAAG 
TCGCTGTCCG CGCTCATCGG CCAGTGCATC CACATTGAGA GAAGGCTGGC CCGGGCTGCC 
GCCACCCGCA AGCCGCGCTC CCCGCCACGC GCGCTGGTGG CGCCGCACGT CAACAATCAC 
CACAACCACC AGGTAGACCC GACTGAGCCC GTGGGGGGCG CCCGCATGCG CCTGACGCAG 
GAAGAGAAAG AGAGACGTCG CAAGCTGAAC CTGTGCCTCT ATTGTGGGAA TGGAGGCCAC 
TACGCTGACA ACTGTCCTGC CAAGGCCTCG AAGGCTTCGC CGGCGGGAAA ACTCCCCGGC 
CCCGCTGTAG AGGGACCTTC AGCGACCGGG CCGGAATTAA TAAGGTCCCC CCAAGATGAT 
GCTGCGTCAT CTCCACACTT GCAAGTGATG CTCCAGATTC ATCTCCCGGG CCGACACAGC 
CTGTTCGTCC GAGCCATGAT CGATTCTGGC GCTTCTGGCA ATTTCATTGA TCACGAGTAC 
GTGGCCCAGA ACGGCATTCC TCTGAGAATC AAGGACTGGC CCATACTTGT AGAAGCAATT 
GATGGACGTC CCATAGCATC GGGCCCCGTG GTCCACGAAA CACATGACCT GATCGTCGAC 
TTGGGAGATC ACCGCGAGGT GCTGTCGTTC GATGTGACTC AGTCTCCGTT CTTCCCCATC 
GTCCTGGGGG TGCGCTGGCT GAGCACACAC GATCCCAACA TCACATGGAG CACCCGATCG 
ATCGTCTTCG ATTCCGAATA TTGCAGATAC CACTGCCGGA TGTATTCTCC GATACCACCA 
CCTCTCCCGC CACCAGCACA ACCGGCGTTC TATTACCCGG TAGAGGGGTA CAGAGTTTAC 
CAGCCTGTGA GATACTACTA CGTGCAGAAT GTGTACACCC CAGTGGATGA GAACGTCTAC 
CCAGACCACC GTCTGGTGGA CCCTAACATA GAAATGATCC CCGGAGCTCA CAGTATTCCC 
AGCGGGCATG TGTACTCACT GTCGGAATCT GAAATGGCAG CCCTGCGAGA TTTCGTCGCC 
AGACATGTGA AGGATGGGCT GATCACTCCC ACCATCGCGC CAAACGGAGC CCAAGTTCTC 
CAAGTGAAAA GAGGGTGGAA GCTGCAAGTT TCTTACGACT GCCGGGGTCC TAACGGTGTC 
ACCATCCAGA ATCAGTATCC TCGACTCACA ATTCCAAATT TAGATGACCA AGCTCACCTG 
GCGACGTACA CTGAATACGT ACCTCAAATT CCAGGATATC CGACCTACCC CGCCTATGCG 
TACCCGACCT ACCCAGTAGG ATTCGCCTGG TACCCCGTGG GGCGAGATGG ACACGGGCGA 
TCCCTCTATG TCCCCGTGAT GATCACCTGG AATCCTCATT GGTACCGCCA GCCGCCAGTG 
CCCCAGTACC CGCCGCCGCA GCCTCCACCG CCGCCACCCC CGCCGCCACC CCCGCCTCCG 
TCTTACAGCG CCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEG10 ( NM_001109815.5 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001103285.1 Bos taurus paternally expressed 10 (PEG10), transcript variant 2, mRNA.

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CloneID	OBa40028
Clone ID Related Accession (Same CDS sequence)	NM_001109815.5
Accession Version	NM_001109815.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-13
Organism	Bos taurus(cattle)
Product	retrotransposon-derived protein PEG10 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV621212.1, EV620062.1, NKLS02000004.1, EE376683.1, EE391829.1, EE371339.1, EE381872.1, DV928493.1, EE376643.1, EE381933.1, CB450408.1 and EH200262.1. On Jun 14, 2012 this sequence version replaced NM_001109815.4. Transcript Variant: This variant (2) has two overlapping open reading frames (RF1 and RF2). It uses an alternate donor splice site at the 5' terminal exon and initiates translation from an alternate, upstream AUG compared to variant 1. This isoform (4) produced from RF1 in the absence of -1 translational frameshifting has a longer and distinct N-terminus, but a shorter C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.46034.1, SRR5571258.1881.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145420 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 21586401 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGAAACA AAAAGCTTCT GAAAACCAGA AGGCGAAAAG GTGGACGCGG AGGCCAGGAC 
ACAGGCCTCC ATTGCCACAG GAGTGAAGCT ACTACGCGCG GGAGGTCTCC TCCCACCCCA 
ACCATCACCC TGGGGCCCGA CTGCCCACCA CCTCCGCCTC CTCCTCCCCC CAACGATCCT 
TCCTCCCCCT CCAACTCCCC CTGCAGCAGC CAGAGGGGCC AGTACAACCC AAACCTGGGA 
GAACGCCGGC GGGAGGATCT CTCAGAGGAG ATCAACAACC TCAGAGAGAA GGTCATGAAG 
CAGTCCGAGG AGAACAACAA CCTGCAGAAC CAGGTGCAGA AACTCACCGA GGAGAACACC 
ACCCTCCGGG AGCAAGTGGA GCCCACCCCC GAGGAGGAAG AGGACGACAT TGAGCTGTGC 
GGTGCAGCGG CCGCCGCCGC CCCAGCCTCC CCTATCGAGG AGGAGTGCCC AGAAGACCTT 
CCCGAGAAGT TCGACGGCAA CCCAGACATG CTGGTTCCCT TCATGTCCCA GTGCCAGCTC 
TTCATGGAGA AGAGCACCCG CGATTTCTCT GTCGATCGCG TGCGCGTCTG CTTCGTGACC 
AGCATGATGA CGGGCCGCGC TGCCCGCTGG GCCTCGGCCA AACTGGAGCG TTCTCACTAT 
CTCATGCACA ACTACCCAGC CTTCATGACC GAAATGAAGC ACGTCTTTGA AGATCCCCAG 
CGGCGCGAGG CTGCCAAACG CAAGATTAGA CGTCTGCGCC AGGGCATGGG GTCAGTGGTC 
GACTACTCTA ATGCTTTCCA GATGATCGCG CAGGACCTGG ATTGGAACGA GCCCGCCCTG 
ATCGACCAGT ACCACGAGGG CCTCAGCGAC CACATCCAGG CAGAGCTGGC GCGCCTCGAA 
GTGGCCAAGT CGCTGTCCGC GCTCATCGGC CAGTGCATCC ACATTGAGAG AAGGCTGGCC 
CGGGCTGCCG CCACCCGCAA GCCGCGCTCC CCGCCACGCG CGCTGGTGGC GCCGCACGTC 
AACAATCACC ACAACCACCA GGTAGACCCG ACTGAGCCCG TGGGGGGCGC CCGCATGCGC 
CTGACGCAGG AAGAGAAAGA GAGACGTCGC AAGCTGAACC TGTGCCTCTA TTGTGGGAAT 
GGAGGCCACT ACGCTGACAA CTGTCCTGCC AAGGCCTCGA AGGCTTCGCC GGCGGGAAAC 
TCCCCGGCCC CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103285.1
CDS	218..1435
Translation	MRNKKLLKTRRRKGGRGGQDTGLHCHRSEATTRGRSPPTPTITLGPDCPPPPPPPPPNDPSSPSNSPCSSQRGQYNPNLGERRREDLSEEINNLREKVMKQSEENNNLQNQVQKLTEENTTLREQVEPTPEEEEDDIELCGAAAAAAPASPIEEECPEDLPEKFDGNPDMLVPFMSQCQLFMEKSTRDFSVDRVRVCFVTSMMTGRAARWASAKLERSHYLMHNYPAFMTEMKHVFEDPQRREAAKRKIRRLRQGMGSVVDYSNAFQMIAQDLDWNEPALIDQYHEGLSDHIQAELARLEVAKSLSALIGQCIHIERRLARAAATRKPRSPPRALVAPHVNNHHNHQVDPTEPVGGARMRLTQEEKERRRKLNLCLYCGNGGHYADNCPAKASKASPAGNSPAPL

Target ORF information:

RefSeq Version	NM_001109815.5
Organism	Bos taurus(cattle)
Definition	Bos taurus paternally expressed 10 (PEG10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109815.5

ORF Insert Sequence:

ATGCGAAACA AAAAGCTTCT GAAAACCAGA AGGCGAAAAG GTGGACGCGG AGGCCAGGAC 
ACAGGCCTCC ATTGCCACAG GAGTGAAGCT ACTACGCGCG GGAGGTCTCC TCCCACCCCA 
ACCATCACCC TGGGGCCCGA CTGCCCACCA CCTCCGCCTC CTCCTCCCCC CAACGATCCT 
TCCTCCCCCT CCAACTCCCC CTGCAGCAGC CAGAGGGGCC AGTACAACCC AAACCTGGGA 
GAACGCCGGC GGGAGGATCT CTCAGAGGAG ATCAACAACC TCAGAGAGAA GGTCATGAAG 
CAGTCCGAGG AGAACAACAA CCTGCAGAAC CAGGTGCAGA AACTCACCGA GGAGAACACC 
ACCCTCCGGG AGCAAGTGGA GCCCACCCCC GAGGAGGAAG AGGACGACAT TGAGCTGTGC 
GGTGCAGCGG CCGCCGCCGC CCCAGCCTCC CCTATCGAGG AGGAGTGCCC AGAAGACCTT 
CCCGAGAAGT TCGACGGCAA CCCAGACATG CTGGTTCCCT TCATGTCCCA GTGCCAGCTC 
TTCATGGAGA AGAGCACCCG CGATTTCTCT GTCGATCGCG TGCGCGTCTG CTTCGTGACC 
AGCATGATGA CGGGCCGCGC TGCCCGCTGG GCCTCGGCCA AACTGGAGCG TTCTCACTAT 
CTCATGCACA ACTACCCAGC CTTCATGACC GAAATGAAGC ACGTCTTTGA AGATCCCCAG 
CGGCGCGAGG CTGCCAAACG CAAGATTAGA CGTCTGCGCC AGGGCATGGG GTCAGTGGTC 
GACTACTCTA ATGCTTTCCA GATGATCGCG CAGGACCTGG ATTGGAACGA GCCCGCCCTG 
ATCGACCAGT ACCACGAGGG CCTCAGCGAC CACATCCAGG CAGAGCTGGC GCGCCTCGAA 
GTGGCCAAGT CGCTGTCCGC GCTCATCGGC CAGTGCATCC ACATTGAGAG AAGGCTGGCC 
CGGGCTGCCG CCACCCGCAA GCCGCGCTCC CCGCCACGCG CGCTGGTGGC GCCGCACGTC 
AACAATCACC ACAACCACCA GGTAGACCCG ACTGAGCCCG TGGGGGGCGC CCGCATGCGC 
CTGACGCAGG AAGAGAAAGA GAGACGTCGC AAGCTGAACC TGTGCCTCTA TTGTGGGAAT 
GGAGGCCACT ACGCTGACAA CTGTCCTGCC AAGGCCTCGA AGGCTTCGCC GGCGGGAAAC 
TCCCCGGCCC CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEG10 ( NM_001172519.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001165990.2 Bos taurus paternally expressed 10 (PEG10), transcript variant 1, mRNA.

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CloneID	OBa56515
Clone ID Related Accession (Same CDS sequence)	NM_001172519.2
Accession Version	NM_001172519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-13
Organism	Bos taurus(cattle)
Product	retrotransposon-derived protein PEG10 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV615229.1, EV620062.1, NKLS02000004.1, EE376683.1, EE391829.1, EE371339.1, EE381872.1, DV928493.1, EE376643.1, EE381933.1, CB450408.1 and EH200262.1. On Jun 14, 2012 this sequence version replaced NM_001172519.1. Transcript Variant: This variant (1) has two overlapping open reading frames (RF1 and RF2) and initiates translation from a non-AUG (CUG) start codon. This isoform (2) produced from RF1 in the absence of -1 translational frameshifting has a shorter C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.46010.1, SRR5571257.56332.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145420 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 21586401 non-AUG initiation codon :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGGGCCCG ACTGCCCACC ACCTCCGCCT CCTCCTCCCC CCAACGATCC TTCCTCCCCC 
TCCAACTCCC CCTGCAGCAG CCAGAGGGGC CAGTACAACC CAAACCTGGG AGAACGCCGG 
CGGGAGGATC TCTCAGAGGA GATCAACAAC CTCAGAGAGA AGGTCATGAA GCAGTCCGAG 
GAGAACAACA ACCTGCAGAA CCAGGTGCAG AAACTCACCG AGGAGAACAC CACCCTCCGG 
GAGCAAGTGG AGCCCACCCC CGAGGAGGAA GAGGACGACA TTGAGCTGTG CGGTGCAGCG 
GCCGCCGCCG CCCCAGCCTC CCCTATCGAG GAGGAGTGCC CAGAAGACCT TCCCGAGAAG 
TTCGACGGCA ACCCAGACAT GCTGGTTCCC TTCATGTCCC AGTGCCAGCT CTTCATGGAG 
AAGAGCACCC GCGATTTCTC TGTCGATCGC GTGCGCGTCT GCTTCGTGAC CAGCATGATG 
ACGGGCCGCG CTGCCCGCTG GGCCTCGGCC AAACTGGAGC GTTCTCACTA TCTCATGCAC 
AACTACCCAG CCTTCATGAC CGAAATGAAG CACGTCTTTG AAGATCCCCA GCGGCGCGAG 
GCTGCCAAAC GCAAGATTAG ACGTCTGCGC CAGGGCATGG GGTCAGTGGT CGACTACTCT 
AATGCTTTCC AGATGATCGC GCAGGACCTG GATTGGAACG AGCCCGCCCT GATCGACCAG 
TACCACGAGG GCCTCAGCGA CCACATCCAG GCAGAGCTGG CGCGCCTCGA AGTGGCCAAG 
TCGCTGTCCG CGCTCATCGG CCAGTGCATC CACATTGAGA GAAGGCTGGC CCGGGCTGCC 
GCCACCCGCA AGCCGCGCTC CCCGCCACGC GCGCTGGTGG CGCCGCACGT CAACAATCAC 
CACAACCACC AGGTAGACCC GACTGAGCCC GTGGGGGGCG CCCGCATGCG CCTGACGCAG 
GAAGAGAAAG AGAGACGTCG CAAGCTGAAC CTGTGCCTCT ATTGTGGGAA TGGAGGCCAC 
TACGCTGACA ACTGTCCTGC CAAGGCCTCG AAGGCTTCGC CGGCGGGAAA CTCCCCGGCC 
CCGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165990.2
CDS	336..1424
Translation	LGPDCPPPPPPPPPNDPSSPSNSPCSSQRGQYNPNLGERRREDLSEEINNLREKVMKQSEENNNLQNQVQKLTEENTTLREQVEPTPEEEEDDIELCGAAAAAAPASPIEEECPEDLPEKFDGNPDMLVPFMSQCQLFMEKSTRDFSVDRVRVCFVTSMMTGRAARWASAKLERSHYLMHNYPAFMTEMKHVFEDPQRREAAKRKIRRLRQGMGSVVDYSNAFQMIAQDLDWNEPALIDQYHEGLSDHIQAELARLEVAKSLSALIGQCIHIERRLARAAATRKPRSPPRALVAPHVNNHHNHQVDPTEPVGGARMRLTQEEKERRRKLNLCLYCGNGGHYADNCPAKASKASPAGNSPAPL

Target ORF information:

RefSeq Version	NM_001172519.2
Organism	Bos taurus(cattle)
Definition	Bos taurus paternally expressed 10 (PEG10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172519.2

ORF Insert Sequence:

CTGGGGCCCG ACTGCCCACC ACCTCCGCCT CCTCCTCCCC CCAACGATCC TTCCTCCCCC 
TCCAACTCCC CCTGCAGCAG CCAGAGGGGC CAGTACAACC CAAACCTGGG AGAACGCCGG 
CGGGAGGATC TCTCAGAGGA GATCAACAAC CTCAGAGAGA AGGTCATGAA GCAGTCCGAG 
GAGAACAACA ACCTGCAGAA CCAGGTGCAG AAACTCACCG AGGAGAACAC CACCCTCCGG 
GAGCAAGTGG AGCCCACCCC CGAGGAGGAA GAGGACGACA TTGAGCTGTG CGGTGCAGCG 
GCCGCCGCCG CCCCAGCCTC CCCTATCGAG GAGGAGTGCC CAGAAGACCT TCCCGAGAAG 
TTCGACGGCA ACCCAGACAT GCTGGTTCCC TTCATGTCCC AGTGCCAGCT CTTCATGGAG 
AAGAGCACCC GCGATTTCTC TGTCGATCGC GTGCGCGTCT GCTTCGTGAC CAGCATGATG 
ACGGGCCGCG CTGCCCGCTG GGCCTCGGCC AAACTGGAGC GTTCTCACTA TCTCATGCAC 
AACTACCCAG CCTTCATGAC CGAAATGAAG CACGTCTTTG AAGATCCCCA GCGGCGCGAG 
GCTGCCAAAC GCAAGATTAG ACGTCTGCGC CAGGGCATGG GGTCAGTGGT CGACTACTCT 
AATGCTTTCC AGATGATCGC GCAGGACCTG GATTGGAACG AGCCCGCCCT GATCGACCAG 
TACCACGAGG GCCTCAGCGA CCACATCCAG GCAGAGCTGG CGCGCCTCGA AGTGGCCAAG 
TCGCTGTCCG CGCTCATCGG CCAGTGCATC CACATTGAGA GAAGGCTGGC CCGGGCTGCC 
GCCACCCGCA AGCCGCGCTC CCCGCCACGC GCGCTGGTGG CGCCGCACGT CAACAATCAC 
CACAACCACC AGGTAGACCC GACTGAGCCC GTGGGGGGCG CCCGCATGCG CCTGACGCAG 
GAAGAGAAAG AGAGACGTCG CAAGCTGAAC CTGTGCCTCT ATTGTGGGAA TGGAGGCCAC 
TACGCTGACA ACTGTCCTGC CAAGGCCTCG AAGGCTTCGC CGGCGGGAAA CTCCCCGGCC 
CCGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[The methylation status of PEG10 in placentas of cloned transgenic calves].
Yi chuan = Hereditas33(5)533-8(2011 May)
Su JM,Xu WB,Li YY,Wang LJ,Wang YS,Zhang Y

Genetic and molecular analyses of PEG10 reveal new aspects of genomic organization, transcription and translation.
PloS one5(1)e8686(2010 Jan)
Lux H,Flammann H,Hafner M,Lux A

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Mammalian gene PEG10 expresses two reading frames by high efficiency -1 frameshifting in embryonic-associated tissues.
The Journal of biological chemistry282(52)37359-69(2007 Dec)
Clark MB,J?nicke M,Gottesb?hren U,Kleffmann T,Legge M,Poole ES,Tate WP

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PEG10 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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