PEG10 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PEG10
Entrez Gene ID	482307
Full Name	paternally expressed 10
General protein information	Preferred Names paternally expressed 10 Names retrotransposon-derived protein PEG10
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)
Summary	This gene is thought to have been derived from the Ty3/Gypsy family of retrotransposons. It contains two overlapping open reading frames, RF1 and RF2, and expresses two proteins: a shorter, gag-like protein (with a CCHC-type zinc finger domain) from RF1; and a longer, gag/pol-like fusion protein (with an additional aspartic protease motif) from RF1/RF2 by -1 translational frameshifting (-1 FS). While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements required for -1 FS. It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation, apoptosis and cancer development. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. Use of non-AUG (CUG) translation initiation codon has been found for this gene. [provided by RefSeq, Oct 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001172544.2	NP_001166015.2	retrotransposon-derived protein PEG10 isoform 2
NM_001172543.2	NP_001166014.2	retrotransposon-derived protein PEG10 isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Peg10	NP_570947.2
Canis lupus familiaris (dog)	PEG10	NP_001166014.2
Homo sapiens (human)	PEG10	NP_001165908.1
Pan troglodytes (chimpanzee)	PEG10	NP_001165897.1
	PEG10	NP_001165893.1
Rattus norvegicus (Norway rat)	LOC102553587	XP_006236145.1
Bos taurus (cattle)	PEG10	NP_001120682.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and molecular analyses of PEG10 reveal new aspects of genomic organization, transcription and translation.
Lux H, Flammann H, Hafner M, Lux A
PloS one5(1)e8686(2010 Jan)

Mammalian gene PEG10 expresses two reading frames by high efficiency -1 frameshifting in embryonic-associated tissues.
Clark MB, J?nicke M, Gottesb?hren U, Kleffmann T, Legge M, Poole ES, Tate WP
The Journal of biological chemistry282(52)37359-69(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PEG10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PEG10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe173669	NM_001172543.2 Latest version!	Canis lupus familiaris paternally expressed 10 (PEG10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe34229	NM_001172544.2 Latest version!	Canis lupus familiaris paternally expressed 10 (PEG10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PEG10 ( NM_001172543.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001166014.2 Canis lupus familiaris paternally expressed 10 (PEG10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OCe173669
Clone ID Related Accession (Same CDS sequence)	NM_001172543.2
Accession Version	NM_001172543.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-13
Organism	Canis lupus familiaris(dog)
Product	retrotransposon-derived protein PEG10 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AAEX03009316.1. On Oct 22, 2014 this sequence version replaced NM_001172543.1. Transcript Variant: This variant (1) has two overlapping open reading frames (RF1 and RF2) and initiates translation from a non-AUG (CUG) start codon. This longer isoform (1) is derived from RF1/RF2 by -1 translational frameshifting. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignment to orthologous human transcripts. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ribosomal slippage :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGGGCCCG ACTGCCCACC TCCTCCTCCT CCTCCTCCCC CCAACGGCCC CTCCTCCTCC 
TCTTCCTCCT GCTCCTCCTC CTCTTCCTCT TCTTCATCCT CACGCAGCGC CCCAAGGGGC 
CAGTACATCC CCAACCTGGC CGAACGAAGG CGAGACGATC TCTCTGAAGA GATCAACAAC 
CTCCGAGAGA AGGTCATGAA GCAGTCCGAG GAGAACAACA ACCTGCAGAA CCAGGTGCAG 
AAGCTCACAG AGGAGAACAC CTCCCTGCGC GAGCAAGTGG AGCCCGCCCC TCAGGACGAG 
GAGGACGACC TGGAGCTCCG AGGTGCTGCA GCCGCCGCTG CCCCACCCAC TCCGCTAGAG 
GAGGAGTGCC CGGACGACCT TCCTGAGAAG TTTGACGGCA ACCCCGACAT GCTGGTCCCG 
TTCATGGCCC AGTGCCAGCT CTTCATGGAA AAGAGCACCA GAGATTTCTC CGTCGACCGC 
GTCCGCGTCT GCTTCGTGAC CAGCATGATG ACGGGCCGTG CCGCCCGCTG GGCCTCCGCC 
AAGCTGGAGC GATCCCACTA CCTGATGCAC AACTACCCCG CCTTCATGAC CGAAATGAAG 
CACGTCTTTG AAGACCCGCA GAGGCGGGAG GCTGCCAAGC GCAAGATCCG ACGTCTGCGC 
CAAGGCATGG GGTCGGTCAT CGACTACTCC AACGCCTTCC AGATGATCGC CCAGGACCTG 
GATTGGAACG AGCCCGCGCT GATCGACCAG TACCACGAGG GCCTCAGCGA CCACATCCAG 
GCCGAGCTGT CGCGCCTCGA GGTCGCCAAG TCGCTGTCCG CCCTGATCGG CCAGTGCATC 
CACATCGAGC GGCGGCTGGC CAGGGCGGCG GCGGCTCGCA AGCCGCGCTC CCCGCCGCGC 
GCGCTCGTCC TGCCGCACAT CACGAGCCAC CACCAGGTGG ACCCCACCGA GCCTGTGGGC 
GGCGCCCGCA TGCGCCTGAC CCAGGAAGAG AAGGAGAGGC GCCGAAAGCT GAACCTGTGC 
CTCTACTGCG GCAACGGGGG TCACTACGCC GACAACTGTC CTGCCAAGGC CTCCAAGGCT 
TCGCCGGCGG GAAAACTCCC CGGCCCCGCT GTAGAGGGAC CTTCAGCGAC CGGGCCAGAA 
ATAATAAGGT CCCCACAAGA TGATGCTTCA TCTCCACACT TGCAAGTGAT GCTCCAGATC 
CATCTCCCGG GCAGACACAC CCTGTTCGTC CGCGCCATGA TCGATTCTGG TGCTTCCGGC 
AACTTCATCG ACCACGAGTA CGTTGCCCAG AACGGAATTC CTCTGAGGGT CAAGGACTGG 
CCAATACTCG TAGAAGCGAT CGATGGGCGT CCCATAGCCT CGGGCCCCGT TGTCCACGAA 
ACGCACGACC TGATAGTCGA CCTGGGAGAT CACCGTGAGG TGCTGTCATT CGACGTGACT 
CAGTCTCCCT TCTTCCCCGT CGTCCTGGGG GTGCGCTGGC TGAGCACACA CGATCCCAAC 
ATCACGTGGA GCACCCGATC CATCGTCTTC GATTCCGAAT ATTGCCGATA CCACTGCCGG 
ATGTATTCCC CAATACCTCC GTCGCTCCCA CCACCAGCAC AGCCGTCCTT TTACTACCCG 
GTGGATGGAT ACCGAGTTTA CCAGCCGGTG AGGTATTACT ACGTCCAGAA TGTGTACACT 
CCGGTGGATG AAAACGTCTA CCCAGACCAC CGCCTGGTCG ACCCGACCAT AGAAATGATC 
CCTGGAGCAC ACAGTATCCC CAGCGGACAC GTGTACTCCC TGTCCGAACC CGAGATGGCA 
GCCCTGCGAG ATTTCGTGGC CAGAAACGTC AAGGATGGGC TCATCACTCC AACCATCGCC 
CCCAACGGAG CCCAAGTTCT CCAGGTGAAA AGGGGGTGGA AACTGCAAGT TTCCTACGAC 
TGCCGAGCTC CCAACAGCTT CACCATCCAG AACCAGTATC CTCGACTCTC CATTCCAAAT 
TTGGATGACC AGGCTCACCT GGCGACGTAC ACGGAGTATG TACCTCAAAT ACCTGGATAC 
CAGACGTACC CCACGTACGC CACGTACCCG ACCTACCCGG TAGGATTCGC CTGGTACCCA 
GTGGGGCGAG ATGGACATGG ACGATCGCTC TATGTCCCCG TGATGATCAC TTGGAATCCG 
CATTGGTACC GCCAGCCTCC GGTGCCCCAG TACCCGCCGC CGCAGCCGCC GCCTCCGCCG 
CCGCCGCCGC CGCCGCCGCC GTCCTACAGC ACCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166014.2
CDS	join(377..1468,1468..2634)
Translation	LGPDCPPPPPPPPPNGPSSSSSSCSSSSSSSSSSRSAPRGQYIPNLAERRRDDLSEEINNLREKVMKQSEENNNLQNQVQKLTEENTSLREQVEPAPQDEEDDLELRGAAAAAAPPTPLEEECPDDLPEKFDGNPDMLVPFMAQCQLFMEKSTRDFSVDRVRVCFVTSMMTGRAARWASAKLERSHYLMHNYPAFMTEMKHVFEDPQRREAAKRKIRRLRQGMGSVIDYSNAFQMIAQDLDWNEPALIDQYHEGLSDHIQAELSRLEVAKSLSALIGQCIHIERRLARAAAARKPRSPPRALVLPHITSHHQVDPTEPVGGARMRLTQEEKERRRKLNLCLYCGNGGHYADNCPAKASKASPAGKLPGPAVEGPSATGPEIIRSPQDDASSPHLQVMLQIHLPGRHTLFVRAMIDSGASGNFIDHEYVAQNGIPLRVKDWPILVEAIDGRPIASGPVVHETHDLIVDLGDHREVLSFDVTQSPFFPVVLGVRWLSTHDPNITWSTRSIVFDSEYCRYHCRMYSPIPPSLPPPAQPSFYYPVDGYRVYQPVRYYYVQNVYTPVDENVYPDHRLVDPTIEMIPGAHSIPSGHVYSLSEPEMAALRDFVARNVKDGLITPTIAPNGAQVLQVKRGWKLQVSYDCRAPNSFTIQNQYPRLSIPNLDDQAHLATYTEYVPQIPGYQTYPTYATYPTYPVGFAWYPVGRDGHGRSLYVPVMITWNPHWYRQPPVPQYPPPQPPPPPPPPPPPPSYSTM

Target ORF information:

RefSeq Version	NM_001172543.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris paternally expressed 10 (PEG10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172543.2

ORF Insert Sequence:

CTGGGGCCCG ACTGCCCACC TCCTCCTCCT CCTCCTCCCC CCAACGGCCC CTCCTCCTCC 
TCTTCCTCCT GCTCCTCCTC CTCTTCCTCT TCTTCATCCT CACGCAGCGC CCCAAGGGGC 
CAGTACATCC CCAACCTGGC CGAACGAAGG CGAGACGATC TCTCTGAAGA GATCAACAAC 
CTCCGAGAGA AGGTCATGAA GCAGTCCGAG GAGAACAACA ACCTGCAGAA CCAGGTGCAG 
AAGCTCACAG AGGAGAACAC CTCCCTGCGC GAGCAAGTGG AGCCCGCCCC TCAGGACGAG 
GAGGACGACC TGGAGCTCCG AGGTGCTGCA GCCGCCGCTG CCCCACCCAC TCCGCTAGAG 
GAGGAGTGCC CGGACGACCT TCCTGAGAAG TTTGACGGCA ACCCCGACAT GCTGGTCCCG 
TTCATGGCCC AGTGCCAGCT CTTCATGGAA AAGAGCACCA GAGATTTCTC CGTCGACCGC 
GTCCGCGTCT GCTTCGTGAC CAGCATGATG ACGGGCCGTG CCGCCCGCTG GGCCTCCGCC 
AAGCTGGAGC GATCCCACTA CCTGATGCAC AACTACCCCG CCTTCATGAC CGAAATGAAG 
CACGTCTTTG AAGACCCGCA GAGGCGGGAG GCTGCCAAGC GCAAGATCCG ACGTCTGCGC 
CAAGGCATGG GGTCGGTCAT CGACTACTCC AACGCCTTCC AGATGATCGC CCAGGACCTG 
GATTGGAACG AGCCCGCGCT GATCGACCAG TACCACGAGG GCCTCAGCGA CCACATCCAG 
GCCGAGCTGT CGCGCCTCGA GGTCGCCAAG TCGCTGTCCG CCCTGATCGG CCAGTGCATC 
CACATCGAGC GGCGGCTGGC CAGGGCGGCG GCGGCTCGCA AGCCGCGCTC CCCGCCGCGC 
GCGCTCGTCC TGCCGCACAT CACGAGCCAC CACCAGGTGG ACCCCACCGA GCCTGTGGGC 
GGCGCCCGCA TGCGCCTGAC CCAGGAAGAG AAGGAGAGGC GCCGAAAGCT GAACCTGTGC 
CTCTACTGCG GCAACGGGGG TCACTACGCC GACAACTGTC CTGCCAAGGC CTCCAAGGCT 
TCGCCGGCGG GAAAACTCCC CGGCCCCGCT GTAGAGGGAC CTTCAGCGAC CGGGCCAGAA 
ATAATAAGGT CCCCACAAGA TGATGCTTCA TCTCCACACT TGCAAGTGAT GCTCCAGATC 
CATCTCCCGG GCAGACACAC CCTGTTCGTC CGCGCCATGA TCGATTCTGG TGCTTCCGGC 
AACTTCATCG ACCACGAGTA CGTTGCCCAG AACGGAATTC CTCTGAGGGT CAAGGACTGG 
CCAATACTCG TAGAAGCGAT CGATGGGCGT CCCATAGCCT CGGGCCCCGT TGTCCACGAA 
ACGCACGACC TGATAGTCGA CCTGGGAGAT CACCGTGAGG TGCTGTCATT CGACGTGACT 
CAGTCTCCCT TCTTCCCCGT CGTCCTGGGG GTGCGCTGGC TGAGCACACA CGATCCCAAC 
ATCACGTGGA GCACCCGATC CATCGTCTTC GATTCCGAAT ATTGCCGATA CCACTGCCGG 
ATGTATTCCC CAATACCTCC GTCGCTCCCA CCACCAGCAC AGCCGTCCTT TTACTACCCG 
GTGGATGGAT ACCGAGTTTA CCAGCCGGTG AGGTATTACT ACGTCCAGAA TGTGTACACT 
CCGGTGGATG AAAACGTCTA CCCAGACCAC CGCCTGGTCG ACCCGACCAT AGAAATGATC 
CCTGGAGCAC ACAGTATCCC CAGCGGACAC GTGTACTCCC TGTCCGAACC CGAGATGGCA 
GCCCTGCGAG ATTTCGTGGC CAGAAACGTC AAGGATGGGC TCATCACTCC AACCATCGCC 
CCCAACGGAG CCCAAGTTCT CCAGGTGAAA AGGGGGTGGA AACTGCAAGT TTCCTACGAC 
TGCCGAGCTC CCAACAGCTT CACCATCCAG AACCAGTATC CTCGACTCTC CATTCCAAAT 
TTGGATGACC AGGCTCACCT GGCGACGTAC ACGGAGTATG TACCTCAAAT ACCTGGATAC 
CAGACGTACC CCACGTACGC CACGTACCCG ACCTACCCGG TAGGATTCGC CTGGTACCCA 
GTGGGGCGAG ATGGACATGG ACGATCGCTC TATGTCCCCG TGATGATCAC TTGGAATCCG 
CATTGGTACC GCCAGCCTCC GGTGCCCCAG TACCCGCCGC CGCAGCCGCC GCCTCCGCCG 
CCGCCGCCGC CGCCGCCGCC GTCCTACAGC ACCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEG10 ( NM_001172544.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001166015.2 Canis lupus familiaris paternally expressed 10 (PEG10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OCe34229
Clone ID Related Accession (Same CDS sequence)	NM_001172544.2
Accession Version	NM_001172544.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-13
Organism	Canis lupus familiaris(dog)
Product	retrotransposon-derived protein PEG10 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AAEX03009316.1. On Oct 22, 2014 this sequence version replaced NM_001172544.1. Transcript Variant: This variant (1) has two overlapping open reading frames (RF1 and RF2) and initiates translation from a non-AUG (CUG) start codon. This isoform (2) produced from RF1 in the absence of -1 translational frameshifting has a shorter C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignment to orthologous human transcripts. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGGGCCCG ACTGCCCACC TCCTCCTCCT CCTCCTCCCC CCAACGGCCC CTCCTCCTCC 
TCTTCCTCCT GCTCCTCCTC CTCTTCCTCT TCTTCATCCT CACGCAGCGC CCCAAGGGGC 
CAGTACATCC CCAACCTGGC CGAACGAAGG CGAGACGATC TCTCTGAAGA GATCAACAAC 
CTCCGAGAGA AGGTCATGAA GCAGTCCGAG GAGAACAACA ACCTGCAGAA CCAGGTGCAG 
AAGCTCACAG AGGAGAACAC CTCCCTGCGC GAGCAAGTGG AGCCCGCCCC TCAGGACGAG 
GAGGACGACC TGGAGCTCCG AGGTGCTGCA GCCGCCGCTG CCCCACCCAC TCCGCTAGAG 
GAGGAGTGCC CGGACGACCT TCCTGAGAAG TTTGACGGCA ACCCCGACAT GCTGGTCCCG 
TTCATGGCCC AGTGCCAGCT CTTCATGGAA AAGAGCACCA GAGATTTCTC CGTCGACCGC 
GTCCGCGTCT GCTTCGTGAC CAGCATGATG ACGGGCCGTG CCGCCCGCTG GGCCTCCGCC 
AAGCTGGAGC GATCCCACTA CCTGATGCAC AACTACCCCG CCTTCATGAC CGAAATGAAG 
CACGTCTTTG AAGACCCGCA GAGGCGGGAG GCTGCCAAGC GCAAGATCCG ACGTCTGCGC 
CAAGGCATGG GGTCGGTCAT CGACTACTCC AACGCCTTCC AGATGATCGC CCAGGACCTG 
GATTGGAACG AGCCCGCGCT GATCGACCAG TACCACGAGG GCCTCAGCGA CCACATCCAG 
GCCGAGCTGT CGCGCCTCGA GGTCGCCAAG TCGCTGTCCG CCCTGATCGG CCAGTGCATC 
CACATCGAGC GGCGGCTGGC CAGGGCGGCG GCGGCTCGCA AGCCGCGCTC CCCGCCGCGC 
GCGCTCGTCC TGCCGCACAT CACGAGCCAC CACCAGGTGG ACCCCACCGA GCCTGTGGGC 
GGCGCCCGCA TGCGCCTGAC CCAGGAAGAG AAGGAGAGGC GCCGAAAGCT GAACCTGTGC 
CTCTACTGCG GCAACGGGGG TCACTACGCC GACAACTGTC CTGCCAAGGC CTCCAAGGCT 
TCGCCGGCGG GAAACTCCCC GGCCCCGCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166015.2
CDS	377..1489
Translation	LGPDCPPPPPPPPPNGPSSSSSSCSSSSSSSSSSRSAPRGQYIPNLAERRRDDLSEEINNLREKVMKQSEENNNLQNQVQKLTEENTSLREQVEPAPQDEEDDLELRGAAAAAAPPTPLEEECPDDLPEKFDGNPDMLVPFMAQCQLFMEKSTRDFSVDRVRVCFVTSMMTGRAARWASAKLERSHYLMHNYPAFMTEMKHVFEDPQRREAAKRKIRRLRQGMGSVIDYSNAFQMIAQDLDWNEPALIDQYHEGLSDHIQAELSRLEVAKSLSALIGQCIHIERRLARAAAARKPRSPPRALVLPHITSHHQVDPTEPVGGARMRLTQEEKERRRKLNLCLYCGNGGHYADNCPAKASKASPAGNSPAPL

Target ORF information:

RefSeq Version	NM_001172544.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris paternally expressed 10 (PEG10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172544.2

ORF Insert Sequence:

CTGGGGCCCG ACTGCCCACC TCCTCCTCCT CCTCCTCCCC CCAACGGCCC CTCCTCCTCC 
TCTTCCTCCT GCTCCTCCTC CTCTTCCTCT TCTTCATCCT CACGCAGCGC CCCAAGGGGC 
CAGTACATCC CCAACCTGGC CGAACGAAGG CGAGACGATC TCTCTGAAGA GATCAACAAC 
CTCCGAGAGA AGGTCATGAA GCAGTCCGAG GAGAACAACA ACCTGCAGAA CCAGGTGCAG 
AAGCTCACAG AGGAGAACAC CTCCCTGCGC GAGCAAGTGG AGCCCGCCCC TCAGGACGAG 
GAGGACGACC TGGAGCTCCG AGGTGCTGCA GCCGCCGCTG CCCCACCCAC TCCGCTAGAG 
GAGGAGTGCC CGGACGACCT TCCTGAGAAG TTTGACGGCA ACCCCGACAT GCTGGTCCCG 
TTCATGGCCC AGTGCCAGCT CTTCATGGAA AAGAGCACCA GAGATTTCTC CGTCGACCGC 
GTCCGCGTCT GCTTCGTGAC CAGCATGATG ACGGGCCGTG CCGCCCGCTG GGCCTCCGCC 
AAGCTGGAGC GATCCCACTA CCTGATGCAC AACTACCCCG CCTTCATGAC CGAAATGAAG 
CACGTCTTTG AAGACCCGCA GAGGCGGGAG GCTGCCAAGC GCAAGATCCG ACGTCTGCGC 
CAAGGCATGG GGTCGGTCAT CGACTACTCC AACGCCTTCC AGATGATCGC CCAGGACCTG 
GATTGGAACG AGCCCGCGCT GATCGACCAG TACCACGAGG GCCTCAGCGA CCACATCCAG 
GCCGAGCTGT CGCGCCTCGA GGTCGCCAAG TCGCTGTCCG CCCTGATCGG CCAGTGCATC 
CACATCGAGC GGCGGCTGGC CAGGGCGGCG GCGGCTCGCA AGCCGCGCTC CCCGCCGCGC 
GCGCTCGTCC TGCCGCACAT CACGAGCCAC CACCAGGTGG ACCCCACCGA GCCTGTGGGC 
GGCGCCCGCA TGCGCCTGAC CCAGGAAGAG AAGGAGAGGC GCCGAAAGCT GAACCTGTGC 
CTCTACTGCG GCAACGGGGG TCACTACGCC GACAACTGTC CTGCCAAGGC CTCCAAGGCT 
TCGCCGGCGG GAAACTCCCC GGCCCCGCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and molecular analyses of PEG10 reveal new aspects of genomic organization, transcription and translation.
PloS one5(1)e8686(2010 Jan)
Lux H,Flammann H,Hafner M,Lux A

Mammalian gene PEG10 expresses two reading frames by high efficiency -1 frameshifting in embryonic-associated tissues.
The Journal of biological chemistry282(52)37359-69(2007 Dec)
Clark MB,J?nicke M,Gottesb?hren U,Kleffmann T,Legge M,Poole ES,Tate WP

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PEG10 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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