Itsn1 cDNA ORF clone, Mus musculus(house mouse)

The following Itsn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itsn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu68032 XM_011246102.3
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu52683 XM_006522931.3
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu52684 XM_006522932.4
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu52686 XM_006522934.4
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu12995 NM_010587.2
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu13054 NM_001110276.1
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OMu13095 NM_001110275.1
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OMu52685 XM_006522933.2
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu52687 XM_006522935.2
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu52688 XM_006522936.2
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu52689 XM_006522937.3
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OMu52690 XM_006522938.2
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OMu52691 XM_006522939.2
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OMu52692 XM_006522940.2
Latest version!
Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
View Old Accession Versions >>
OMu68032 XM_011246102.2 Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OMu52683 XM_006522931.2 Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu52686 XM_006522934.3 Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu52684 XM_006522932.3 Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu68032
    Clone ID Related Accession (Same CDS sequence) XM_011246102.2 , XM_011246102.3
    Accession Version XM_011246102.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246102.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGGGTGAAA AGGTGGAAGG GCTACAAGCG
    CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA GACAACCACT TAAATTTTAA CAAAAGTGAC
    GTCATCACCG TTCTGGAACA GCAAGACATG TGGTGGTTTG GAGAAGTTCA AGCATCGATA
    CTGGCCCTAC TGAAAGTCCT GCTAGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011244404.1
    CDS428..3397
    Translation

    Target ORF information:

    RefSeq Version XM_011246102.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011246102.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGGGTGAAA AGGTGGAAGG GCTACAAGCG
    CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA GACAACCACT TAAATTTTAA CAAAAGTGAC
    GTCATCACCG TTCTGGAACA GCAAGACATG TGGTGGTTTG GAGAAGTTCA AGCATCGATA
    CTGGCCCTAC TGAAAGTCCT GCTAGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52683
    Clone ID Related Accession (Same CDS sequence) XM_006522931.3 , XM_006522931.2
    Accession Version XM_006522931.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5232bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGGGTGAAA AGGTGGAAGG GCTACAAGCG
    CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA GACAACCACT TAAATTTTAA CAAAAGTGAC
    GTCATCACCG TTCTGGAACA GCAAGACATG TGGTGGTTTG GAGAAGTTCA AGGTCAGAAG
    GGTTGGTTCC CCAAGTCTTA CGTGAAACTC ATTTCAGGGC CCGTAAGGAA ATCCACAAGC
    ATCGATACTG GCCCTACTGA AAGTCCTGCT AGTCTAAAGA GAGTGGCTTC CCCGGCCGCC
    AAGCCAGCCA TTCCCGGAGA AGAGTTTATT GCCATGTACA CATACGAGAG TTCTGAGCAA
    GGAGATTTAA CCTTTCAGCA AGGGGATGTG ATTGTGGTTA CCAAGAAAGA TGGTGACTGG
    TGGACGGGAA CGGTGGGCGA CAAGTCCGGA GTCTTCCCTT CTAACTATGT GAGGCTTAAA
    GATTCAGAGG GCTCTGGAAC TGCTGGGAAA ACAGGGAGTT TAGGAAAAAA ACCTGAAATT
    GCCCAGGTTA TTGCTTCCTA CGCTGCTACT GGTCCCGAAC AACTCACCCT GGCTCCTGGG
    CAGCTGATTC TGATCCGGAA AAAGAACCCA GGTGGATGGT GGGAAGGAGA ACTGCAAGCT
    CGAGGGAAAA AGCGCCAGAT AGGGTGGTTT CCAGCAAATT ATGTCAAACT TCTAAGCCCC
    GGAACAAGCA AAATCACCCC AACTGAGCTA CCCAAGACCG CAGTGCAGCC AGCAGTGTGC
    CAGGTGATCG GGATGTACGA TTACACCGCC CAGAACGATG ACGAACTAGC CTTCAGCAAA
    GGCCAGATCA TCAACGTCCT CAACAAGGAG GACCCGGACT GGTGGAAAGG AGAAGTCAGT
    GGGCAAGTTG GGCTCTTCCC ATCCAATTAT GTAAAGCTGA CCACAGACAT GGACCCCAGC
    CAGCAATGGT GCTCAGACCT GCATCTCTTA GATATGCTGA CCCCGACTGA GAGGAAGCGG
    CAAGGCTACA TCCATGAACT CATTGTCACG GAGGAGAACT ACGTGAACGA CTTGCAGCTG
    GTCACAGAGA TCTTTCAGAA ACCCCTGACG GAGTCTGAGC TGCTGACAGA AAAAGAGGTT
    GCTATGATTT TTGTTAACTG GAAGGAGCTG ATCATGTGTA ATATCAAACT GCTGAAAGCG
    CTGAGAGTCC GCAAGAAGAT GTCTGGGGAG AAGATGCCGG TGAAGATGAT TGGCGACATC
    CTGAGCGCCC AGCTGCCGCA CATGCAGCCT TACATCCGCT TCTGCAGCTG CCAGCTCAAT
    GGGGCTGCCC TCATCCAGCA GAAGACGGAC GAGGCTCCAG ACTTCAAGGA GTTCGTCAAA
    AGACTGGCAA TGGACCCTCG GTGCAAAGGA ATGCCTCTGT CCAGCTTTAT ACTGAAGCCT
    ATGCAGCGTG TCACAAGATA CCCGCTGATC ATTAAAAACA TCCTGGAAAA CACTCCCGAG
    AACCATCCAG ACCACAGCCA CCTGAAGCAT GCCCTGGAAA AGGCGGAGGA GCTATGCTCC
    CAGGTGAACG AGGGAGTTCG AGAGAAGGAG AACTCAGACC GGCTGGAGTG GATCCAAGCC
    CACGTGCAGT GTGAAGGCCT TTCTGAGCAA CTGGTGTTCA ATTCAGTGAC CAACTGCTTG
    GGACCACGCA AGTTTCTGCA CAGCGGGAAG CTCTACAAGG CCAAGAGCAA CAAAGAACTG
    TATGGCTTCC TCTTCAACGA CTTCCTCCTG CTGACCCAAA TCACAAAGCC CTTAGGCTCT
    TCCGGCACCG ACAAAGTCTT CAGCCCCAAA TCTAACCTTC AGTATAAAAT GTACAAAACG
    CCCATTTTCT TAAATGAGGT TCTAGTAAAA TTGCCCACGG ACCCTTCTGG AGATGAGCCT
    ATCTTCCACA TTTCCCACAT CGACCGGGTC TACACCCTCC GAGCAGAGAG CATAAATGAG
    AGGACTGCCT GGGTGCAGAA AATCAAGGCG GCGTCTGAGC TCTACATAGA GACGGAGAAA
    AAGAAGCGAG AGAAGGCGTA CCTGGTCCGT TCCCAGCGGG CGACCGGTAT TGGAAGGTTG
    ATGGTGAACG TGGTAGAAGG CATTGAGCTG AAGCCCTGTC GGTCACATGG AAAGAGCAAC
    CCGTACTGTG AGGTGACCAT GGGCTCTCAG TGCCACATCA CCAAGACAAT CCAGGACACG
    CTAAACCCCA AGTGGAATTC TAACTGCCAG TTCTTCATCA GAGACCTGGA GCAGGAGGTT
    CTCTGCATCA CAGTGTTTGA GAGGGACCAG TTCTCGCCTG ATGATTTTTT GGGTCGGACA
    GAGATCCGAG TGGCCGACAT CAAGAAAGAC CAGGGCTCCA AGGGGCCGGT TACGAAGTGT
    CTCCTGCTGC ATGAGGTCCC CACGGGAGAG ATTGTGGTCC GCCTTGACCT GCAGTTGTTT
    GATGAGCCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522994.1
    CDS929..6160
    Translation

    Target ORF information:

    RefSeq Version XM_006522931.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522931.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGGGTGAAA AGGTGGAAGG GCTACAAGCG
    CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA GACAACCACT TAAATTTTAA CAAAAGTGAC
    GTCATCACCG TTCTGGAACA GCAAGACATG TGGTGGTTTG GAGAAGTTCA AGGTCAGAAG
    GGTTGGTTCC CCAAGTCTTA CGTGAAACTC ATTTCAGGGC CCGTAAGGAA ATCCACAAGC
    ATCGATACTG GCCCTACTGA AAGTCCTGCT AGTCTAAAGA GAGTGGCTTC CCCGGCCGCC
    AAGCCAGCCA TTCCCGGAGA AGAGTTTATT GCCATGTACA CATACGAGAG TTCTGAGCAA
    GGAGATTTAA CCTTTCAGCA AGGGGATGTG ATTGTGGTTA CCAAGAAAGA TGGTGACTGG
    TGGACGGGAA CGGTGGGCGA CAAGTCCGGA GTCTTCCCTT CTAACTATGT GAGGCTTAAA
    GATTCAGAGG GCTCTGGAAC TGCTGGGAAA ACAGGGAGTT TAGGAAAAAA ACCTGAAATT
    GCCCAGGTTA TTGCTTCCTA CGCTGCTACT GGTCCCGAAC AACTCACCCT GGCTCCTGGG
    CAGCTGATTC TGATCCGGAA AAAGAACCCA GGTGGATGGT GGGAAGGAGA ACTGCAAGCT
    CGAGGGAAAA AGCGCCAGAT AGGGTGGTTT CCAGCAAATT ATGTCAAACT TCTAAGCCCC
    GGAACAAGCA AAATCACCCC AACTGAGCTA CCCAAGACCG CAGTGCAGCC AGCAGTGTGC
    CAGGTGATCG GGATGTACGA TTACACCGCC CAGAACGATG ACGAACTAGC CTTCAGCAAA
    GGCCAGATCA TCAACGTCCT CAACAAGGAG GACCCGGACT GGTGGAAAGG AGAAGTCAGT
    GGGCAAGTTG GGCTCTTCCC ATCCAATTAT GTAAAGCTGA CCACAGACAT GGACCCCAGC
    CAGCAATGGT GCTCAGACCT GCATCTCTTA GATATGCTGA CCCCGACTGA GAGGAAGCGG
    CAAGGCTACA TCCATGAACT CATTGTCACG GAGGAGAACT ACGTGAACGA CTTGCAGCTG
    GTCACAGAGA TCTTTCAGAA ACCCCTGACG GAGTCTGAGC TGCTGACAGA AAAAGAGGTT
    GCTATGATTT TTGTTAACTG GAAGGAGCTG ATCATGTGTA ATATCAAACT GCTGAAAGCG
    CTGAGAGTCC GCAAGAAGAT GTCTGGGGAG AAGATGCCGG TGAAGATGAT TGGCGACATC
    CTGAGCGCCC AGCTGCCGCA CATGCAGCCT TACATCCGCT TCTGCAGCTG CCAGCTCAAT
    GGGGCTGCCC TCATCCAGCA GAAGACGGAC GAGGCTCCAG ACTTCAAGGA GTTCGTCAAA
    AGACTGGCAA TGGACCCTCG GTGCAAAGGA ATGCCTCTGT CCAGCTTTAT ACTGAAGCCT
    ATGCAGCGTG TCACAAGATA CCCGCTGATC ATTAAAAACA TCCTGGAAAA CACTCCCGAG
    AACCATCCAG ACCACAGCCA CCTGAAGCAT GCCCTGGAAA AGGCGGAGGA GCTATGCTCC
    CAGGTGAACG AGGGAGTTCG AGAGAAGGAG AACTCAGACC GGCTGGAGTG GATCCAAGCC
    CACGTGCAGT GTGAAGGCCT TTCTGAGCAA CTGGTGTTCA ATTCAGTGAC CAACTGCTTG
    GGACCACGCA AGTTTCTGCA CAGCGGGAAG CTCTACAAGG CCAAGAGCAA CAAAGAACTG
    TATGGCTTCC TCTTCAACGA CTTCCTCCTG CTGACCCAAA TCACAAAGCC CTTAGGCTCT
    TCCGGCACCG ACAAAGTCTT CAGCCCCAAA TCTAACCTTC AGTATAAAAT GTACAAAACG
    CCCATTTTCT TAAATGAGGT TCTAGTAAAA TTGCCCACGG ACCCTTCTGG AGATGAGCCT
    ATCTTCCACA TTTCCCACAT CGACCGGGTC TACACCCTCC GAGCAGAGAG CATAAATGAG
    AGGACTGCCT GGGTGCAGAA AATCAAGGCG GCGTCTGAGC TCTACATAGA GACGGAGAAA
    AAGAAGCGAG AGAAGGCGTA CCTGGTCCGT TCCCAGCGGG CGACCGGTAT TGGAAGGTTG
    ATGGTGAACG TGGTAGAAGG CATTGAGCTG AAGCCCTGTC GGTCACATGG AAAGAGCAAC
    CCGTACTGTG AGGTGACCAT GGGCTCTCAG TGCCACATCA CCAAGACAAT CCAGGACACG
    CTAAACCCCA AGTGGAATTC TAACTGCCAG TTCTTCATCA GAGACCTGGA GCAGGAGGTT
    CTCTGCATCA CAGTGTTTGA GAGGGACCAG TTCTCGCCTG ATGATTTTTT GGGTCGGACA
    GAGATCCGAG TGGCCGACAT CAAGAAAGAC CAGGGCTCCA AGGGGCCGGT TACGAAGTGT
    CTCCTGCTGC ATGAGGTCCC CACGGGAGAG ATTGTGGTCC GCCTTGACCT GCAGTTGTTT
    GATGAGCCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52684
    Clone ID Related Accession (Same CDS sequence) XM_006522932.3 , XM_006522932.4
    Accession Version XM_006522932.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522932.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGACACA TTTTCACAGC TTTCTCCAAA CTCTGCGTCG ATCAGCAAGG TGAACGTAAT 
    AGAACCATGG CTCAGTTTCC CACACCTTTC GGTGGTAGCC TGGATGTCTG GGCCATAACT
    GTGGAGGAAA GGGCCAAGCA TGACCAGCAG TTCCTTAGCC TGAAGCCGAT AGCGGGATTT
    ATTACTGGTG ATCAAGCGAG GAACTTTTTT TTCCAATCTG GGTTACCTCA GCCTGTCTTA
    GCACAAATAT GGGCGCTAGC GGACATGAAT AACGATGGAA GGATGGATCA AGTGGAATTT
    TCCATAGCCA TGAAGCTTAT CAAACTGAAG CTACAAGGAT ATCAGCTCCC CTCCACACTT
    CCCCCTGTCA TGAAACAGCA ACCAGTGGCT ATTTCCAGTG CACCAGCATT TGGTATAGGA
    GGGATTGCTA GCATGCCACC ACTCACAGCT GTTGCTCCTG TGCCAATGGG CTCCATTCCA
    GTTGTTGGAA TGTCTCCACC CTTAGTATCT TCTGTCCCTC CAGCAGCAGT GCCTCCCCTG
    GCTAACGGGG CTCCTCCCGT CATACAGCCT CTGCCTGCGT TTGCGCATCC TGCAGCCACA
    TTGCCAAAGA GTTCTTCCTT CAGCAGATCT GGTCCAGGGT CACAATTAAA CACTAAGTTA
    CAGAAGGCAC AATCATTCGA TGTCGCCAGC GCCCCTCCAG CAGCAGAATG GGCTGTGCCT
    CAGTCATCAA GGCTGAAATA CAGGCAGTTA TTCAACAGCC ACGACAAAAC TATGAGTGGA
    CACTTAACAG GTCCCCAGGC AAGAACTATT CTCATGCAAT CAAGTTTACC CCAGGCTCAG
    CTGGCTTCAA TATGGAATCT TTCTGACATT GATCAAGATG GAAAACTCAC TGCAGAAGAA
    TTTATCCTAG CTATGCACCT AATTGATGTT GCCATGTCTG GTCAGCCACT GCCGCCCGTC
    CTGCCTCCAG AATACATCCC TCCTTCCTTC AGAAGAGTTC GCTCCGGCAG TGGGATGTCC
    GTCATAAGCT CTTCTTCTGT GGATCAGAGG CTGCCTGAGG AGCCGTCGTC AGAGGATGAG
    CAGCAGCCAG AGAAGAAACT GCCTGTGACA TTTGAAGATA AGAAGCGGGA GAACTTCGAG
    CGAGGCAGTG TGGAGCTGGA GAAGCGCCGC CAAGCGCTCT TGGAGCAGCA GCGCAAAGAG
    CAGGAGCGGT TGGCTCAGCT GGAGCGCGCC GAGCAGGAGA GGAAAGAGCG GGAGCGCCAG
    GAGCAGGAGC GCAAGCGGCA GCTGGAGCTG GAGAAGCAGC TGGAGAAGCA GCGGGAGCTG
    GAGCGGCAGC GAGAGGAGGA GAGGAGGAAG GAGATCGAGA GGCGCGAGGC CGCAAAACGG
    GAACTGGAAA GGCAGCGACA ACTTGAATGG GAACGGAACC GGAGACAGGA ACTCCTGAAT
    CAGAGGAACA AGGAGCAGGA GGGCACCGTG GTCCTGAAGG CAAGGAGGAA GACTCTGGAG
    TTTGAGTTAG AAGCTCTGAA TGACAAAAAG CATCAGCTAG AAGGAAAACT TCAGGATATC
    AGGTGTCGAC TGGCAACCCA GAGGCAAGAA ATTGAGAGCA CGAACAAGTC TAGAGAGCTA
    AGAATTGCTG AAATCACCCA CTTACAGCAG CAGTTGCAGG AATCTCAGCA AATGCTTGGA
    AGACTTATTC CAGAGAAACA GATACTCAGT GACCAGTTAA AACAAGTCCA GCAGAACAGT
    TTGCATAGAG ACTCGCTTCT TACCCTCAAA AGAGCCTTGG AAGCAAAGGA GCTGGCCCGG
    CAGCAGCTCC GGGAGCAGCT GGACGAGGTG GAGAGAGAGA CCAGGTCAAA GCTGCAGGAG
    ATTGATGTTT TCAACAACCA GCTGAAGGAA CTGAGAGAGA TACATAGCAA ACAGCAACTC
    CAGAAGCAGA GGTCCCTGGA GGCAGCGCGA CTGAAGCAGA AAGAGCAGGA GAGGAAGAGC
    CTGGAGTTAG AGAAGCAAAA GGAAGACGCT CAGAGACGAG TTCAGGAAAG GGACAAGCAA
    TGGCTGGAGC ATGTGCAGCA GGAGGAGCAG CCACGCCCCC GGAAACCCCA CGAGGAGGAC
    AGACTGAAGA GGGAAGACAG TGTCAGGAAG AAGGAGGCGG AAGAGAGAGC CAAGCCGGAA
    ATGCAAGACA AGCAGAGTCG GCTTTTCCAT CCGCATCAGG AGCCAGCTAA GCTGGCCACC
    CAGGCACCCT GGTCTACCAC AGAGAAAGGC CCGCTTACCA TTTCTGCACA GGAGAGTGTA
    AAAGTGGTAT ATTACCGAGC GCTGTACCCC TTTGAATCCA GAAGTCACGA TGAGATCACC
    ATCCAGCCAG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAGACTGGA
    GAGCCAGGAT GGCTTGGAGG AGAGCTGAAA GGGAAGACGG GATGGTTCCC TGCAAACTAT
    GCAGAAAAGA TTCCAGAAAA TGAGGTTCCC ACTCCAGCCA AACCAGTGAC CGATCTGACA
    TCTGCCCCTG CCCCCAAACT GGCTCTGCGT GAGACCCCTG CTCCTTTGCC AGTGACCTCT
    TCTGAGCCCT CCACAACCCC CAACAACTGG GCAGACTTCA GTTCCACGTG GCCCAGCAGC
    TCAAACGAGA AGCCAGAAAC GGACAACTGG GATACATGGG CGGCTCAGCC TTCTCTGACC
    GTACCTAGTG CTGGCCAGTT ACGGCAGAGA TCAGCCTTTA CCCCAGCCAC AGCCACTGGC
    TCCTCCCCAT CTCCCGTCCT GGGCCAGGGT GAAAAGGTGG AAGGGCTACA AGCGCAAGCC
    CTGTATCCCT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAG TGACGTCATC
    ACCGTTCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCGTAA GGAAATCCAC AAGCATCGAT
    ACTGGCCCTA CTGAAAGTCC TGCTAGTCTA AAGAGAGTGG CTTCCCCGGC CGCCAAGCCA
    GCCATTCCCG GAGAAGAGTT TATTGCCATG TACACATACG AGAGTTCTGA GCAAGGAGAT
    TTAACCTTTC AGCAAGGGGA TGTGATTGTG GTTACCAAGA AAGATGGTGA CTGGTGGACG
    GGAACGGTGG GCGACAAGTC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG
    GTTATTGCTT CCTACGCTGC TACTGGTCCC GAACAACTCA CCCTGGCTCC TGGGCAGCTG
    ATTCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAACTGCA AGCTCGAGGG
    AAAAAGCGCC AGATAGGGTG GTTTCCAGCA AATTATGTCA AACTTCTAAG CCCCGGAACA
    AGCAAAATCA CCCCAACTGA GCTACCCAAG ACCGCAGTGC AGCCAGCAGT GTGCCAGGTG
    ATCGGGATGT ACGATTACAC CGCCCAGAAC GATGACGAAC TAGCCTTCAG CAAAGGCCAG
    ATCATCAACG TCCTCAACAA GGAGGACCCG GACTGGTGGA AAGGAGAAGT CAGTGGGCAA
    GTTGGGCTCT TCCCATCCAA TTATGTAAAG CTGACCACAG ACATGGACCC CAGCCAGCAA
    TGGTGCTCAG ACCTGCATCT CTTAGATATG CTGACCCCGA CTGAGAGGAA GCGGCAAGGC
    TACATCCATG AACTCATTGT CACGGAGGAG AACTACGTGA ACGACTTGCA GCTGGTCACA
    GAGATCTTTC AGAAACCCCT GACGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG
    ATTTTTGTTA ACTGGAAGGA GCTGATCATG TGTAATATCA AACTGCTGAA AGCGCTGAGA
    GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCGGTGAAGA TGATTGGCGA CATCCTGAGC
    GCCCAGCTGC CGCACATGCA GCCTTACATC CGCTTCTGCA GCTGCCAGCT CAATGGGGCT
    GCCCTCATCC AGCAGAAGAC GGACGAGGCT CCAGACTTCA AGGAGTTCGT CAAAAGACTG
    GCAATGGACC CTCGGTGCAA AGGAATGCCT CTGTCCAGCT TTATACTGAA GCCTATGCAG
    CGTGTCACAA GATACCCGCT GATCATTAAA AACATCCTGG AAAACACTCC CGAGAACCAT
    CCAGACCACA GCCACCTGAA GCATGCCCTG GAAAAGGCGG AGGAGCTATG CTCCCAGGTG
    AACGAGGGAG TTCGAGAGAA GGAGAACTCA GACCGGCTGG AGTGGATCCA AGCCCACGTG
    CAGTGTGAAG GCCTTTCTGA GCAACTGGTG TTCAATTCAG TGACCAACTG CTTGGGACCA
    CGCAAGTTTC TGCACAGCGG GAAGCTCTAC AAGGCCAAGA GCAACAAAGA ACTGTATGGC
    TTCCTCTTCA ACGACTTCCT CCTGCTGACC CAAATCACAA AGCCCTTAGG CTCTTCCGGC
    ACCGACAAAG TCTTCAGCCC CAAATCTAAC CTTCAGTATA AAATGTACAA AACGCCCATT
    TTCTTAAATG AGGTTCTAGT AAAATTGCCC ACGGACCCTT CTGGAGATGA GCCTATCTTC
    CACATTTCCC ACATCGACCG GGTCTACACC CTCCGAGCAG AGAGCATAAA TGAGAGGACT
    GCCTGGGTGC AGAAAATCAA GGCGGCGTCT GAGCTCTACA TAGAGACGGA GAAAAAGAAG
    CGAGAGAAGG CGTACCTGGT CCGTTCCCAG CGGGCGACCG GTATTGGAAG GTTGATGGTG
    AACGTGGTAG AAGGCATTGA GCTGAAGCCC TGTCGGTCAC ATGGAAAGAG CAACCCGTAC
    TGTGAGGTGA CCATGGGCTC TCAGTGCCAC ATCACCAAGA CAATCCAGGA CACGCTAAAC
    CCCAAGTGGA ATTCTAACTG CCAGTTCTTC ATCAGAGACC TGGAGCAGGA GGTTCTCTGC
    ATCACAGTGT TTGAGAGGGA CCAGTTCTCG CCTGATGATT TTTTGGGTCG GACAGAGATC
    CGAGTGGCCG ACATCAAGAA AGACCAGGGC TCCAAGGGGC CGGTTACGAA GTGTCTCCTG
    CTGCATGAGG TCCCCACGGG AGAGATTGTG GTCCGCCTTG ACCTGCAGTT GTTTGATGAG
    CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522995.1
    CDS7864..13089
    Translation

    Target ORF information:

    RefSeq Version XM_006522932.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522932.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGACACA TTTTCACAGC TTTCTCCAAA CTCTGCGTCG ATCAGCAAGG TGAACGTAAT 
    AGAACCATGG CTCAGTTTCC CACACCTTTC GGTGGTAGCC TGGATGTCTG GGCCATAACT
    GTGGAGGAAA GGGCCAAGCA TGACCAGCAG TTCCTTAGCC TGAAGCCGAT AGCGGGATTT
    ATTACTGGTG ATCAAGCGAG GAACTTTTTT TTCCAATCTG GGTTACCTCA GCCTGTCTTA
    GCACAAATAT GGGCGCTAGC GGACATGAAT AACGATGGAA GGATGGATCA AGTGGAATTT
    TCCATAGCCA TGAAGCTTAT CAAACTGAAG CTACAAGGAT ATCAGCTCCC CTCCACACTT
    CCCCCTGTCA TGAAACAGCA ACCAGTGGCT ATTTCCAGTG CACCAGCATT TGGTATAGGA
    GGGATTGCTA GCATGCCACC ACTCACAGCT GTTGCTCCTG TGCCAATGGG CTCCATTCCA
    GTTGTTGGAA TGTCTCCACC CTTAGTATCT TCTGTCCCTC CAGCAGCAGT GCCTCCCCTG
    GCTAACGGGG CTCCTCCCGT CATACAGCCT CTGCCTGCGT TTGCGCATCC TGCAGCCACA
    TTGCCAAAGA GTTCTTCCTT CAGCAGATCT GGTCCAGGGT CACAATTAAA CACTAAGTTA
    CAGAAGGCAC AATCATTCGA TGTCGCCAGC GCCCCTCCAG CAGCAGAATG GGCTGTGCCT
    CAGTCATCAA GGCTGAAATA CAGGCAGTTA TTCAACAGCC ACGACAAAAC TATGAGTGGA
    CACTTAACAG GTCCCCAGGC AAGAACTATT CTCATGCAAT CAAGTTTACC CCAGGCTCAG
    CTGGCTTCAA TATGGAATCT TTCTGACATT GATCAAGATG GAAAACTCAC TGCAGAAGAA
    TTTATCCTAG CTATGCACCT AATTGATGTT GCCATGTCTG GTCAGCCACT GCCGCCCGTC
    CTGCCTCCAG AATACATCCC TCCTTCCTTC AGAAGAGTTC GCTCCGGCAG TGGGATGTCC
    GTCATAAGCT CTTCTTCTGT GGATCAGAGG CTGCCTGAGG AGCCGTCGTC AGAGGATGAG
    CAGCAGCCAG AGAAGAAACT GCCTGTGACA TTTGAAGATA AGAAGCGGGA GAACTTCGAG
    CGAGGCAGTG TGGAGCTGGA GAAGCGCCGC CAAGCGCTCT TGGAGCAGCA GCGCAAAGAG
    CAGGAGCGGT TGGCTCAGCT GGAGCGCGCC GAGCAGGAGA GGAAAGAGCG GGAGCGCCAG
    GAGCAGGAGC GCAAGCGGCA GCTGGAGCTG GAGAAGCAGC TGGAGAAGCA GCGGGAGCTG
    GAGCGGCAGC GAGAGGAGGA GAGGAGGAAG GAGATCGAGA GGCGCGAGGC CGCAAAACGG
    GAACTGGAAA GGCAGCGACA ACTTGAATGG GAACGGAACC GGAGACAGGA ACTCCTGAAT
    CAGAGGAACA AGGAGCAGGA GGGCACCGTG GTCCTGAAGG CAAGGAGGAA GACTCTGGAG
    TTTGAGTTAG AAGCTCTGAA TGACAAAAAG CATCAGCTAG AAGGAAAACT TCAGGATATC
    AGGTGTCGAC TGGCAACCCA GAGGCAAGAA ATTGAGAGCA CGAACAAGTC TAGAGAGCTA
    AGAATTGCTG AAATCACCCA CTTACAGCAG CAGTTGCAGG AATCTCAGCA AATGCTTGGA
    AGACTTATTC CAGAGAAACA GATACTCAGT GACCAGTTAA AACAAGTCCA GCAGAACAGT
    TTGCATAGAG ACTCGCTTCT TACCCTCAAA AGAGCCTTGG AAGCAAAGGA GCTGGCCCGG
    CAGCAGCTCC GGGAGCAGCT GGACGAGGTG GAGAGAGAGA CCAGGTCAAA GCTGCAGGAG
    ATTGATGTTT TCAACAACCA GCTGAAGGAA CTGAGAGAGA TACATAGCAA ACAGCAACTC
    CAGAAGCAGA GGTCCCTGGA GGCAGCGCGA CTGAAGCAGA AAGAGCAGGA GAGGAAGAGC
    CTGGAGTTAG AGAAGCAAAA GGAAGACGCT CAGAGACGAG TTCAGGAAAG GGACAAGCAA
    TGGCTGGAGC ATGTGCAGCA GGAGGAGCAG CCACGCCCCC GGAAACCCCA CGAGGAGGAC
    AGACTGAAGA GGGAAGACAG TGTCAGGAAG AAGGAGGCGG AAGAGAGAGC CAAGCCGGAA
    ATGCAAGACA AGCAGAGTCG GCTTTTCCAT CCGCATCAGG AGCCAGCTAA GCTGGCCACC
    CAGGCACCCT GGTCTACCAC AGAGAAAGGC CCGCTTACCA TTTCTGCACA GGAGAGTGTA
    AAAGTGGTAT ATTACCGAGC GCTGTACCCC TTTGAATCCA GAAGTCACGA TGAGATCACC
    ATCCAGCCAG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAGACTGGA
    GAGCCAGGAT GGCTTGGAGG AGAGCTGAAA GGGAAGACGG GATGGTTCCC TGCAAACTAT
    GCAGAAAAGA TTCCAGAAAA TGAGGTTCCC ACTCCAGCCA AACCAGTGAC CGATCTGACA
    TCTGCCCCTG CCCCCAAACT GGCTCTGCGT GAGACCCCTG CTCCTTTGCC AGTGACCTCT
    TCTGAGCCCT CCACAACCCC CAACAACTGG GCAGACTTCA GTTCCACGTG GCCCAGCAGC
    TCAAACGAGA AGCCAGAAAC GGACAACTGG GATACATGGG CGGCTCAGCC TTCTCTGACC
    GTACCTAGTG CTGGCCAGTT ACGGCAGAGA TCAGCCTTTA CCCCAGCCAC AGCCACTGGC
    TCCTCCCCAT CTCCCGTCCT GGGCCAGGGT GAAAAGGTGG AAGGGCTACA AGCGCAAGCC
    CTGTATCCCT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAG TGACGTCATC
    ACCGTTCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCGTAA GGAAATCCAC AAGCATCGAT
    ACTGGCCCTA CTGAAAGTCC TGCTAGTCTA AAGAGAGTGG CTTCCCCGGC CGCCAAGCCA
    GCCATTCCCG GAGAAGAGTT TATTGCCATG TACACATACG AGAGTTCTGA GCAAGGAGAT
    TTAACCTTTC AGCAAGGGGA TGTGATTGTG GTTACCAAGA AAGATGGTGA CTGGTGGACG
    GGAACGGTGG GCGACAAGTC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG
    GTTATTGCTT CCTACGCTGC TACTGGTCCC GAACAACTCA CCCTGGCTCC TGGGCAGCTG
    ATTCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAACTGCA AGCTCGAGGG
    AAAAAGCGCC AGATAGGGTG GTTTCCAGCA AATTATGTCA AACTTCTAAG CCCCGGAACA
    AGCAAAATCA CCCCAACTGA GCTACCCAAG ACCGCAGTGC AGCCAGCAGT GTGCCAGGTG
    ATCGGGATGT ACGATTACAC CGCCCAGAAC GATGACGAAC TAGCCTTCAG CAAAGGCCAG
    ATCATCAACG TCCTCAACAA GGAGGACCCG GACTGGTGGA AAGGAGAAGT CAGTGGGCAA
    GTTGGGCTCT TCCCATCCAA TTATGTAAAG CTGACCACAG ACATGGACCC CAGCCAGCAA
    TGGTGCTCAG ACCTGCATCT CTTAGATATG CTGACCCCGA CTGAGAGGAA GCGGCAAGGC
    TACATCCATG AACTCATTGT CACGGAGGAG AACTACGTGA ACGACTTGCA GCTGGTCACA
    GAGATCTTTC AGAAACCCCT GACGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG
    ATTTTTGTTA ACTGGAAGGA GCTGATCATG TGTAATATCA AACTGCTGAA AGCGCTGAGA
    GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCGGTGAAGA TGATTGGCGA CATCCTGAGC
    GCCCAGCTGC CGCACATGCA GCCTTACATC CGCTTCTGCA GCTGCCAGCT CAATGGGGCT
    GCCCTCATCC AGCAGAAGAC GGACGAGGCT CCAGACTTCA AGGAGTTCGT CAAAAGACTG
    GCAATGGACC CTCGGTGCAA AGGAATGCCT CTGTCCAGCT TTATACTGAA GCCTATGCAG
    CGTGTCACAA GATACCCGCT GATCATTAAA AACATCCTGG AAAACACTCC CGAGAACCAT
    CCAGACCACA GCCACCTGAA GCATGCCCTG GAAAAGGCGG AGGAGCTATG CTCCCAGGTG
    AACGAGGGAG TTCGAGAGAA GGAGAACTCA GACCGGCTGG AGTGGATCCA AGCCCACGTG
    CAGTGTGAAG GCCTTTCTGA GCAACTGGTG TTCAATTCAG TGACCAACTG CTTGGGACCA
    CGCAAGTTTC TGCACAGCGG GAAGCTCTAC AAGGCCAAGA GCAACAAAGA ACTGTATGGC
    TTCCTCTTCA ACGACTTCCT CCTGCTGACC CAAATCACAA AGCCCTTAGG CTCTTCCGGC
    ACCGACAAAG TCTTCAGCCC CAAATCTAAC CTTCAGTATA AAATGTACAA AACGCCCATT
    TTCTTAAATG AGGTTCTAGT AAAATTGCCC ACGGACCCTT CTGGAGATGA GCCTATCTTC
    CACATTTCCC ACATCGACCG GGTCTACACC CTCCGAGCAG AGAGCATAAA TGAGAGGACT
    GCCTGGGTGC AGAAAATCAA GGCGGCGTCT GAGCTCTACA TAGAGACGGA GAAAAAGAAG
    CGAGAGAAGG CGTACCTGGT CCGTTCCCAG CGGGCGACCG GTATTGGAAG GTTGATGGTG
    AACGTGGTAG AAGGCATTGA GCTGAAGCCC TGTCGGTCAC ATGGAAAGAG CAACCCGTAC
    TGTGAGGTGA CCATGGGCTC TCAGTGCCAC ATCACCAAGA CAATCCAGGA CACGCTAAAC
    CCCAAGTGGA ATTCTAACTG CCAGTTCTTC ATCAGAGACC TGGAGCAGGA GGTTCTCTGC
    ATCACAGTGT TTGAGAGGGA CCAGTTCTCG CCTGATGATT TTTTGGGTCG GACAGAGATC
    CGAGTGGCCG ACATCAAGAA AGACCAGGGC TCCAAGGGGC CGGTTACGAA GTGTCTCCTG
    CTGCATGAGG TCCCCACGGG AGAGATTGTG GTCCGCCTTG ACCTGCAGTT GTTTGATGAG
    CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52686
    Clone ID Related Accession (Same CDS sequence) XM_006522934.4 , XM_006522934.3
    Accession Version XM_006522934.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5160bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522934.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCCACACC TTTCGGTGGT AGCCTGGATG TCTGGGCCAT AACTGTGGAG 
    GAAAGGGCCA AGCATGACCA GCAGTTCCTT AGCCTGAAGC CGATAGCGGG ATTTATTACT
    GGTGATCAAG CGAGGAACTT TTTTTTCCAA TCTGGGTTAC CTCAGCCTGT CTTAGCACAA
    ATATGGGCGC TAGCGGACAT GAATAACGAT GGAAGGATGG ATCAAGTGGA ATTTTCCATA
    GCCATGAAGC TTATCAAACT GAAGCTACAA GGATATCAGC TCCCCTCCAC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT GGCTATTTCC AGTGCACCAG CATTTGGTAT AGGAGGGATT
    GCTAGCATGC CACCACTCAC AGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCAGTTGTT
    GGAATGTCTC CACCCTTAGT ATCTTCTGTC CCTCCAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCTC CCGTCATACA GCCTCTGCCT GCGTTTGCGC ATCCTGCAGC CACATTGCCA
    AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAATCAT TCGATGTCGC CAGCGCCCCT CCAGCAGCAG AATGGGCTGT GCCTCAGTCA
    TCAAGGCTGA AATACAGGCA GTTATTCAAC AGCCACGACA AAACTATGAG TGGACACTTA
    ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACTGCAGA AGAATTTATC
    CTAGCTATGC ACCTAATTGA TGTTGCCATG TCTGGTCAGC CACTGCCGCC CGTCCTGCCT
    CCAGAATACA TCCCTCCTTC CTTCAGAAGA GTTCGCTCCG GCAGTGGGAT GTCCGTCATA
    AGCTCTTCTT CTGTGGATCA GAGGCTGCCT GAGGAGCCGT CGTCAGAGGA TGAGCAGCAG
    CCAGAGAAGA AACTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC
    AGTGTGGAGC TGGAGAAGCG CCGCCAAGCG CTCTTGGAGC AGCAGCGCAA AGAGCAGGAG
    CGGTTGGCTC AGCTGGAGCG CGCCGAGCAG GAGAGGAAAG AGCGGGAGCG CCAGGAGCAG
    GAGCGCAAGC GGCAGCTGGA GCTGGAGAAG CAGCTGGAGA AGCAGCGGGA GCTGGAGCGG
    CAGCGAGAGG AGGAGAGGAG GAAGGAGATC GAGAGGCGCG AGGCCGCAAA ACGGGAACTG
    GAAAGGCAGC GACAACTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GAATCAGAGG
    AACAAGGAGC AGGAGGGCAC CGTGGTCCTG AAGGCAAGGA GGAAGACTCT GGAGTTTGAG
    TTAGAAGCTC TGAATGACAA AAAGCATCAG CTAGAAGGAA AACTTCAGGA TATCAGGTGT
    CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACGAACA AGTCTAGAGA GCTAAGAATT
    GCTGAAATCA CCCACTTACA GCAGCAGTTG CAGGAATCTC AGCAAATGCT TGGAAGACTT
    ATTCCAGAGA AACAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGTTTGCAT
    AGAGACTCGC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAGCTGGC CCGGCAGCAG
    CTCCGGGAGC AGCTGGACGA GGTGGAGAGA GAGACCAGGT CAAAGCTGCA GGAGATTGAT
    GTTTTCAACA ACCAGCTGAA GGAACTGAGA GAGATACATA GCAAACAGCA ACTCCAGAAG
    CAGAGGTCCC TGGAGGCAGC GCGACTGAAG CAGAAAGAGC AGGAGAGGAA GAGCCTGGAG
    TTAGAGAAGC AAAAGGAAGA CGCTCAGAGA CGAGTTCAGG AAAGGGACAA GCAATGGCTG
    GAGCATGTGC AGCAGGAGGA GCAGCCACGC CCCCGGAAAC CCCACGAGGA GGACAGACTG
    AAGAGGGAAG ACAGTGTCAG GAAGAAGGAG GCGGAAGAGA GAGCCAAGCC GGAAATGCAA
    GACAAGCAGA GTCGGCTTTT CCATCCGCAT CAGGAGCCAG CTAAGCTGGC CACCCAGGCA
    CCCTGGTCTA CCACAGAGAA AGGCCCGCTT ACCATTTCTG CACAGGAGAG TGTAAAAGTG
    GTATATTACC GAGCGCTGTA CCCCTTTGAA TCCAGAAGTC ACGATGAGAT CACCATCCAG
    CCAGGAGATA TAGTCATGGT TAAAGGGGAA TGGGTGGATG AAAGCCAGAC TGGAGAGCCA
    GGATGGCTTG GAGGAGAGCT GAAAGGGAAG ACGGGATGGT TCCCTGCAAA CTATGCAGAA
    AAGATTCCAG AAAATGAGGT TCCCACTCCA GCCAAACCAG TGACCGATCT GACATCTGCC
    CCTGCCCCCA AACTGGCTCT GCGTGAGACC CCTGCTCCTT TGCCAGTGAC CTCTTCTGAG
    CCCTCCACAA CCCCCAACAA CTGGGCAGAC TTCAGTTCCA CGTGGCCCAG CAGCTCAAAC
    GAGAAGCCAG AAACGGACAA CTGGGATACA TGGGCGGCTC AGCCTTCTCT GACCGTACCT
    AGTGCTGGCC AGTTACGGCA GAGATCAGCC TTTACCCCAG CCACAGCCAC TGGCTCCTCC
    CCATCTCCCG TCCTGGGCCA GGGTGAAAAG GTGGAAGGGC TACAAGCGCA AGCCCTGTAT
    CCCTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAGTGACGT CATCACCGTT
    CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC
    AAGTCTTACG TGAAACTCAT TTCAGGGCCC GTAAGGAAAT CCACAAGCAT CGATACTGGC
    CCTACTGAAA GTCCTGCTAG TCTAAAGAGA GTGGCTTCCC CGGCCGCCAA GCCAGCCATT
    CCCGGAGAAG AGTTTATTGC CATGTACACA TACGAGAGTT CTGAGCAAGG AGATTTAACC
    TTTCAGCAAG GGGATGTGAT TGTGGTTACC AAGAAAGATG GTGACTGGTG GACGGGAACG
    GTGGGCGACA AGTCCGGAGT CTTCCCTTCT AACTATGTGA GGCTTAAAGA TTCAGAGGGC
    TCTGGAACTG CTGGGAAAAC AGGGAGTTTA GGAAAAAAAC CTGAAATTGC CCAGGTTATT
    GCTTCCTACG CTGCTACTGG TCCCGAACAA CTCACCCTGG CTCCTGGGCA GCTGATTCTG
    ATCCGGAAAA AGAACCCAGG TGGATGGTGG GAAGGAGAAC TGCAAGCTCG AGGGAAAAAG
    CGCCAGATAG GGTGGTTTCC AGCAAATTAT GTCAAACTTC TAAGCCCCGG AACAAGCAAA
    ATCACCCCAA CTGAGCTACC CAAGACCGCA GTGCAGCCAG CAGTGTGCCA GGTGATCGGG
    ATGTACGATT ACACCGCCCA GAACGATGAC GAACTAGCCT TCAGCAAAGG CCAGATCATC
    AACGTCCTCA ACAAGGAGGA CCCGGACTGG TGGAAAGGAG AAGTCAGTGG GCAAGTTGGG
    CTCTTCCCAT CCAATTATGT AAAGCTGACC ACAGACATGG ACCCCAGCCA GCAATGGTGC
    TCAGACCTGC ATCTCTTAGA TATGCTGACC CCGACTGAGA GGAAGCGGCA AGGCTACATC
    CATGAACTCA TTGTCACGGA GGAGAACTAC GTGAACGACT TGCAGCTGGT CACAGAGATC
    TTTCAGAAAC CCCTGACGGA GTCTGAGCTG CTGACAGAAA AAGAGGTTGC TATGATTTTT
    GTTAACTGGA AGGAGCTGAT CATGTGTAAT ATCAAACTGC TGAAAGCGCT GAGAGTCCGC
    AAGAAGATGT CTGGGGAGAA GATGCCGGTG AAGATGATTG GCGACATCCT GAGCGCCCAG
    CTGCCGCACA TGCAGCCTTA CATCCGCTTC TGCAGCTGCC AGCTCAATGG GGCTGCCCTC
    ATCCAGCAGA AGACGGACGA GGCTCCAGAC TTCAAGGAGT TCGTCAAAAG ACTGGCAATG
    GACCCTCGGT GCAAAGGAAT GCCTCTGTCC AGCTTTATAC TGAAGCCTAT GCAGCGTGTC
    ACAAGATACC CGCTGATCAT TAAAAACATC CTGGAAAACA CTCCCGAGAA CCATCCAGAC
    CACAGCCACC TGAAGCATGC CCTGGAAAAG GCGGAGGAGC TATGCTCCCA GGTGAACGAG
    GGAGTTCGAG AGAAGGAGAA CTCAGACCGG CTGGAGTGGA TCCAAGCCCA CGTGCAGTGT
    GAAGGCCTTT CTGAGCAACT GGTGTTCAAT TCAGTGACCA ACTGCTTGGG ACCACGCAAG
    TTTCTGCACA GCGGGAAGCT CTACAAGGCC AAGAGCAACA AAGAACTGTA TGGCTTCCTC
    TTCAACGACT TCCTCCTGCT GACCCAAATC ACAAAGCCCT TAGGCTCTTC CGGCACCGAC
    AAAGTCTTCA GCCCCAAATC TAACCTTCAG TATAAAATGT ACAAAACGCC CATTTTCTTA
    AATGAGGTTC TAGTAAAATT GCCCACGGAC CCTTCTGGAG ATGAGCCTAT CTTCCACATT
    TCCCACATCG ACCGGGTCTA CACCCTCCGA GCAGAGAGCA TAAATGAGAG GACTGCCTGG
    GTGCAGAAAA TCAAGGCGGC GTCTGAGCTC TACATAGAGA CGGAGAAAAA GAAGCGAGAG
    AAGGCGTACC TGGTCCGTTC CCAGCGGGCG ACCGGTATTG GAAGGTTGAT GGTGAACGTG
    GTAGAAGGCA TTGAGCTGAA GCCCTGTCGG TCACATGGAA AGAGCAACCC GTACTGTGAG
    GTGACCATGG GCTCTCAGTG CCACATCACC AAGACAATCC AGGACACGCT AAACCCCAAG
    TGGAATTCTA ACTGCCAGTT CTTCATCAGA GACCTGGAGC AGGAGGTTCT CTGCATCACA
    GTGTTTGAGA GGGACCAGTT CTCGCCTGAT GATTTTTTGG GTCGGACAGA GATCCGAGTG
    GCCGACATCA AGAAAGACCA GGGCTCCAAG GGGCCGGTTA CGAAGTGTCT CCTGCTGCAT
    GAGGTCCCCA CGGGAGAGAT TGTGGTCCGC CTTGACCTGC AGTTGTTTGA TGAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522997.1
    CDS323..5482
    Translation

    Target ORF information:

    RefSeq Version XM_006522934.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522934.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCCACACC TTTCGGTGGT AGCCTGGATG TCTGGGCCAT AACTGTGGAG 
    GAAAGGGCCA AGCATGACCA GCAGTTCCTT AGCCTGAAGC CGATAGCGGG ATTTATTACT
    GGTGATCAAG CGAGGAACTT TTTTTTCCAA TCTGGGTTAC CTCAGCCTGT CTTAGCACAA
    ATATGGGCGC TAGCGGACAT GAATAACGAT GGAAGGATGG ATCAAGTGGA ATTTTCCATA
    GCCATGAAGC TTATCAAACT GAAGCTACAA GGATATCAGC TCCCCTCCAC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT GGCTATTTCC AGTGCACCAG CATTTGGTAT AGGAGGGATT
    GCTAGCATGC CACCACTCAC AGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCAGTTGTT
    GGAATGTCTC CACCCTTAGT ATCTTCTGTC CCTCCAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCTC CCGTCATACA GCCTCTGCCT GCGTTTGCGC ATCCTGCAGC CACATTGCCA
    AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAATCAT TCGATGTCGC CAGCGCCCCT CCAGCAGCAG AATGGGCTGT GCCTCAGTCA
    TCAAGGCTGA AATACAGGCA GTTATTCAAC AGCCACGACA AAACTATGAG TGGACACTTA
    ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACTGCAGA AGAATTTATC
    CTAGCTATGC ACCTAATTGA TGTTGCCATG TCTGGTCAGC CACTGCCGCC CGTCCTGCCT
    CCAGAATACA TCCCTCCTTC CTTCAGAAGA GTTCGCTCCG GCAGTGGGAT GTCCGTCATA
    AGCTCTTCTT CTGTGGATCA GAGGCTGCCT GAGGAGCCGT CGTCAGAGGA TGAGCAGCAG
    CCAGAGAAGA AACTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC
    AGTGTGGAGC TGGAGAAGCG CCGCCAAGCG CTCTTGGAGC AGCAGCGCAA AGAGCAGGAG
    CGGTTGGCTC AGCTGGAGCG CGCCGAGCAG GAGAGGAAAG AGCGGGAGCG CCAGGAGCAG
    GAGCGCAAGC GGCAGCTGGA GCTGGAGAAG CAGCTGGAGA AGCAGCGGGA GCTGGAGCGG
    CAGCGAGAGG AGGAGAGGAG GAAGGAGATC GAGAGGCGCG AGGCCGCAAA ACGGGAACTG
    GAAAGGCAGC GACAACTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GAATCAGAGG
    AACAAGGAGC AGGAGGGCAC CGTGGTCCTG AAGGCAAGGA GGAAGACTCT GGAGTTTGAG
    TTAGAAGCTC TGAATGACAA AAAGCATCAG CTAGAAGGAA AACTTCAGGA TATCAGGTGT
    CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACGAACA AGTCTAGAGA GCTAAGAATT
    GCTGAAATCA CCCACTTACA GCAGCAGTTG CAGGAATCTC AGCAAATGCT TGGAAGACTT
    ATTCCAGAGA AACAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGTTTGCAT
    AGAGACTCGC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAGCTGGC CCGGCAGCAG
    CTCCGGGAGC AGCTGGACGA GGTGGAGAGA GAGACCAGGT CAAAGCTGCA GGAGATTGAT
    GTTTTCAACA ACCAGCTGAA GGAACTGAGA GAGATACATA GCAAACAGCA ACTCCAGAAG
    CAGAGGTCCC TGGAGGCAGC GCGACTGAAG CAGAAAGAGC AGGAGAGGAA GAGCCTGGAG
    TTAGAGAAGC AAAAGGAAGA CGCTCAGAGA CGAGTTCAGG AAAGGGACAA GCAATGGCTG
    GAGCATGTGC AGCAGGAGGA GCAGCCACGC CCCCGGAAAC CCCACGAGGA GGACAGACTG
    AAGAGGGAAG ACAGTGTCAG GAAGAAGGAG GCGGAAGAGA GAGCCAAGCC GGAAATGCAA
    GACAAGCAGA GTCGGCTTTT CCATCCGCAT CAGGAGCCAG CTAAGCTGGC CACCCAGGCA
    CCCTGGTCTA CCACAGAGAA AGGCCCGCTT ACCATTTCTG CACAGGAGAG TGTAAAAGTG
    GTATATTACC GAGCGCTGTA CCCCTTTGAA TCCAGAAGTC ACGATGAGAT CACCATCCAG
    CCAGGAGATA TAGTCATGGT TAAAGGGGAA TGGGTGGATG AAAGCCAGAC TGGAGAGCCA
    GGATGGCTTG GAGGAGAGCT GAAAGGGAAG ACGGGATGGT TCCCTGCAAA CTATGCAGAA
    AAGATTCCAG AAAATGAGGT TCCCACTCCA GCCAAACCAG TGACCGATCT GACATCTGCC
    CCTGCCCCCA AACTGGCTCT GCGTGAGACC CCTGCTCCTT TGCCAGTGAC CTCTTCTGAG
    CCCTCCACAA CCCCCAACAA CTGGGCAGAC TTCAGTTCCA CGTGGCCCAG CAGCTCAAAC
    GAGAAGCCAG AAACGGACAA CTGGGATACA TGGGCGGCTC AGCCTTCTCT GACCGTACCT
    AGTGCTGGCC AGTTACGGCA GAGATCAGCC TTTACCCCAG CCACAGCCAC TGGCTCCTCC
    CCATCTCCCG TCCTGGGCCA GGGTGAAAAG GTGGAAGGGC TACAAGCGCA AGCCCTGTAT
    CCCTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAGTGACGT CATCACCGTT
    CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC
    AAGTCTTACG TGAAACTCAT TTCAGGGCCC GTAAGGAAAT CCACAAGCAT CGATACTGGC
    CCTACTGAAA GTCCTGCTAG TCTAAAGAGA GTGGCTTCCC CGGCCGCCAA GCCAGCCATT
    CCCGGAGAAG AGTTTATTGC CATGTACACA TACGAGAGTT CTGAGCAAGG AGATTTAACC
    TTTCAGCAAG GGGATGTGAT TGTGGTTACC AAGAAAGATG GTGACTGGTG GACGGGAACG
    GTGGGCGACA AGTCCGGAGT CTTCCCTTCT AACTATGTGA GGCTTAAAGA TTCAGAGGGC
    TCTGGAACTG CTGGGAAAAC AGGGAGTTTA GGAAAAAAAC CTGAAATTGC CCAGGTTATT
    GCTTCCTACG CTGCTACTGG TCCCGAACAA CTCACCCTGG CTCCTGGGCA GCTGATTCTG
    ATCCGGAAAA AGAACCCAGG TGGATGGTGG GAAGGAGAAC TGCAAGCTCG AGGGAAAAAG
    CGCCAGATAG GGTGGTTTCC AGCAAATTAT GTCAAACTTC TAAGCCCCGG AACAAGCAAA
    ATCACCCCAA CTGAGCTACC CAAGACCGCA GTGCAGCCAG CAGTGTGCCA GGTGATCGGG
    ATGTACGATT ACACCGCCCA GAACGATGAC GAACTAGCCT TCAGCAAAGG CCAGATCATC
    AACGTCCTCA ACAAGGAGGA CCCGGACTGG TGGAAAGGAG AAGTCAGTGG GCAAGTTGGG
    CTCTTCCCAT CCAATTATGT AAAGCTGACC ACAGACATGG ACCCCAGCCA GCAATGGTGC
    TCAGACCTGC ATCTCTTAGA TATGCTGACC CCGACTGAGA GGAAGCGGCA AGGCTACATC
    CATGAACTCA TTGTCACGGA GGAGAACTAC GTGAACGACT TGCAGCTGGT CACAGAGATC
    TTTCAGAAAC CCCTGACGGA GTCTGAGCTG CTGACAGAAA AAGAGGTTGC TATGATTTTT
    GTTAACTGGA AGGAGCTGAT CATGTGTAAT ATCAAACTGC TGAAAGCGCT GAGAGTCCGC
    AAGAAGATGT CTGGGGAGAA GATGCCGGTG AAGATGATTG GCGACATCCT GAGCGCCCAG
    CTGCCGCACA TGCAGCCTTA CATCCGCTTC TGCAGCTGCC AGCTCAATGG GGCTGCCCTC
    ATCCAGCAGA AGACGGACGA GGCTCCAGAC TTCAAGGAGT TCGTCAAAAG ACTGGCAATG
    GACCCTCGGT GCAAAGGAAT GCCTCTGTCC AGCTTTATAC TGAAGCCTAT GCAGCGTGTC
    ACAAGATACC CGCTGATCAT TAAAAACATC CTGGAAAACA CTCCCGAGAA CCATCCAGAC
    CACAGCCACC TGAAGCATGC CCTGGAAAAG GCGGAGGAGC TATGCTCCCA GGTGAACGAG
    GGAGTTCGAG AGAAGGAGAA CTCAGACCGG CTGGAGTGGA TCCAAGCCCA CGTGCAGTGT
    GAAGGCCTTT CTGAGCAACT GGTGTTCAAT TCAGTGACCA ACTGCTTGGG ACCACGCAAG
    TTTCTGCACA GCGGGAAGCT CTACAAGGCC AAGAGCAACA AAGAACTGTA TGGCTTCCTC
    TTCAACGACT TCCTCCTGCT GACCCAAATC ACAAAGCCCT TAGGCTCTTC CGGCACCGAC
    AAAGTCTTCA GCCCCAAATC TAACCTTCAG TATAAAATGT ACAAAACGCC CATTTTCTTA
    AATGAGGTTC TAGTAAAATT GCCCACGGAC CCTTCTGGAG ATGAGCCTAT CTTCCACATT
    TCCCACATCG ACCGGGTCTA CACCCTCCGA GCAGAGAGCA TAAATGAGAG GACTGCCTGG
    GTGCAGAAAA TCAAGGCGGC GTCTGAGCTC TACATAGAGA CGGAGAAAAA GAAGCGAGAG
    AAGGCGTACC TGGTCCGTTC CCAGCGGGCG ACCGGTATTG GAAGGTTGAT GGTGAACGTG
    GTAGAAGGCA TTGAGCTGAA GCCCTGTCGG TCACATGGAA AGAGCAACCC GTACTGTGAG
    GTGACCATGG GCTCTCAGTG CCACATCACC AAGACAATCC AGGACACGCT AAACCCCAAG
    TGGAATTCTA ACTGCCAGTT CTTCATCAGA GACCTGGAGC AGGAGGTTCT CTGCATCACA
    GTGTTTGAGA GGGACCAGTT CTCGCCTGAT GATTTTTTGG GTCGGACAGA GATCCGAGTG
    GCCGACATCA AGAAAGACCA GGGCTCCAAG GGGCCGGTTA CGAAGTGTCT CCTGCTGCAT
    GAGGTCCCCA CGGGAGAGAT TGTGGTCCGC CTTGACCTGC AGTTGTTTGA TGAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu12995
    Clone ID Related Accession (Same CDS sequence) NM_010587.2
    Accession Version NM_010587.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC062938.1, AF132481.1, AK082606.1 and AC126053.3. On Mar 30, 2007 this sequence version replaced NM_010587.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF132481.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCCACACC TTTCGGTGGT AGCCTGGATG TCTGGGCCAT AACTGTGGAG 
    GAAAGGGCCA AGCATGACCA GCAGTTCCTT AGCCTGAAGC CGATAGCGGG ATTTATTACT
    GGTGATCAAG CGAGGAACTT TTTTTTCCAA TCTGGGTTAC CTCAGCCTGT CTTAGCACAA
    ATATGGGCGC TAGCGGACAT GAATAACGAT GGAAGGATGG ATCAAGTGGA ATTTTCCATA
    GCCATGAAGC TTATCAAACT GAAGCTACAA GGATATCAGC TCCCCTCCAC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT GGCTATTTCC AGTGCACCAG CATTTGGTAT AGGAGGGATT
    GCTAGCATGC CACCACTCAC AGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCAGTTGTT
    GGAATGTCTC CACCCTTAGT ATCTTCTGTC CCTCCAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCTC CCGTCATACA GCCTCTGCCT GCGTTTGCGC ATCCTGCAGC CACATTGCCA
    AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAATCAT TCGATGTCGC CAGCGCCCCT CCAGCAGCAG AATGGGCTGT GCCTCAGTCA
    TCAAGGCTGA AATACAGGCA GTTATTCAAC AGCCACGACA AAACTATGAG TGGACACTTA
    ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACTGCAGA AGAATTTATC
    CTAGCTATGC ACCTAATTGA TGTTGCCATG TCTGGTCAGC CACTGCCGCC CGTCCTGCCT
    CCAGAATACA TCCCTCCTTC CTTCAGAAGA GTTCGCTCCG GCAGTGGGAT GTCCGTCATA
    AGCTCTTCTT CTGTGGATCA GAGGCTGCCT GAGGAGCCGT CGTCAGAGGA TGAGCAGCAG
    CCAGAGAAGA AACTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC
    AGTGTGGAGC TGGAGAAGCG CCGCCAAGCG CTCTTGGAGC AGCAGCGCAA AGAGCAGGAG
    CGGTTGGCTC AGCTGGAGCG CGCCGAGCAG GAGAGGAAAG AGCGGGAGCG CCAGGAGCAG
    GAGCGCAAGC GGCAGCTGGA GCTGGAGAAG CAGCTGGAGA AGCAGCGGGA GCTGGAGCGG
    CAGCGAGAGG AGGAGAGGAG GAAGGAGATC GAGAGGCGCG AGGCCGCAAA ACGGGAACTG
    GAAAGGCAGC GACAACTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GAATCAGAGG
    AACAAGGAGC AGGAGGGCAC CGTGGTCCTG AAGGCAAGGA GGAAGACTCT GGAGTTTGAG
    TTAGAAGCTC TGAATGACAA AAAGCATCAG CTAGAAGGAA AACTTCAGGA TATCAGGTGT
    CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACGAACA AGTCTAGAGA GCTAAGAATT
    GCTGAAATCA CCCACTTACA GCAGCAGTTG CAGGAATCTC AGCAAATGCT TGGAAGACTT
    ATTCCAGAGA AACAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGTTTGCAT
    AGAGACTCGC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAGCTGGC CCGGCAGCAG
    CTCCGGGAGC AGCTGGACGA GGTGGAGAGA GAGACCAGGT CAAAGCTGCA GGAGATTGAT
    GTTTTCAACA ACCAGCTGAA GGAACTGAGA GAGATACATA GCAAACAGCA ACTCCAGAAG
    CAGAGGTCCC TGGAGGCAGC GCGACTGAAG CAGAAAGAGC AGGAGAGGAA GAGCCTGGAG
    TTAGAGAAGC AAAAGGAAGA CGCTCAGAGA CGAGTTCAGG AAAGGGACAA GCAATGGCTG
    GAGCATGTGC AGCAGGAGGA GCAGCCACGC CCCCGGAAAC CCCACGAGGA GGACAGACTG
    AAGAGGGAAG ACAGTGTCAG GAAGAAGGAG GCGGAAGAGA GAGCCAAGCC GGAAATGCAA
    GACAAGCAGA GTCGGCTTTT CCATCCGCAT CAGGAGCCAG CTAAGCTGGC CACCCAGGCA
    CCCTGGTCTA CCACAGAGAA AGGCCCGCTT ACCATTTCTG CACAGGAGAG TGTAAAAGTG
    GTATATTACC GAGCGCTGTA CCCCTTTGAA TCCAGAAGTC ACGATGAGAT CACCATCCAG
    CCAGGAGATA TAGTCATGGT GGATGAAAGC CAGACTGGAG AGCCAGGATG GCTTGGAGGA
    GAGCTGAAAG GGAAGACGGG ATGGTTCCCT GCAAACTATG CAGAAAAGAT TCCAGAAAAT
    GAGGTTCCCA CTCCAGCCAA ACCAGTGACC GATCTGACAT CTGCCCCTGC CCCCAAACTG
    GCTCTGCGTG AGACCCCTGC TCCTTTGCCA GTGACCTCTT CTGAGCCCTC CACAACCCCC
    AACAACTGGG CAGACTTCAG TTCCACGTGG CCCAGCAGCT CAAACGAGAA GCCAGAAACG
    GACAACTGGG ATACATGGGC GGCTCAGCCT TCTCTGACCG TACCTAGTGC TGGCCAGTTA
    CGGCAGAGAT CAGCCTTTAC CCCAGCCACA GCCACTGGCT CCTCCCCATC TCCCGTCCTG
    GGCCAGGGTG AAAAGGTGGA AGGGCTACAA GCGCAAGCCC TGTATCCCTG GAGAGCCAAA
    AAAGACAACC ACTTAAATTT TAACAAAAGT GACGTCATCA CCGTTCTGGA ACAGCAAGAC
    ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG AAGGGTTGGT TCCCCAAGTC TTACGTGAAA
    CTCATTTCAG GGCCCGTAAG GAAATCCACA AGCATCGATA CTGGCCCTAC TGAAAGTCCT
    GCTAGTCTAA AGAGAGTGGC TTCCCCGGCC GCCAAGCCAG CCATTCCCGG AGAAGAGTTT
    ATTGCCATGT ACACATACGA GAGTTCTGAG CAAGGAGATT TAACCTTTCA GCAAGGGGAT
    GTGATTGTGG TTACCAAGAA AGATGGTGAC TGGTGGACGG GAACGGTGGG CGACAAGTCC
    GGAGTCTTCC CTTCTAACTA TGTGAGGCTT AAAGATTCAG AGGGCTCTGG AACTGCTGGG
    AAAACAGGGA GTTTAGGAAA AAAACCTGAA ATTGCCCAGG TTATTGCTTC CTACGCTGCT
    ACTGGTCCCG AACAACTCAC CCTGGCTCCT GGGCAGCTGA TTCTGATCCG GAAAAAGAAC
    CCAGGTGGAT GGTGGGAAGG AGAACTGCAA GCTCGAGGGA AAAAGCGCCA GATAGGGTGG
    TTTCCAGCAA ATTATGTCAA ACTTCTAAGC CCCGGAACAA GCAAAATCAC CCCAACTGAG
    CTACCCAAGA CCGCAGTGCA GCCAGCAGTG TGCCAGGTGA TCGGGATGTA CGATTACACC
    GCCCAGAACG ATGACGAACT AGCCTTCAGC AAAGGCCAGA TCATCAACGT CCTCAACAAG
    GAGGACCCGG ACTGGTGGAA AGGAGAAGTC AGTGGGCAAG TTGGGCTCTT CCCATCCAAT
    TATGTAAAGC TGACCACAGA CATGGACCCC AGCCAGCAAT GGTGCTCAGA CCTGCATCTC
    TTAGATATGC TGACCCCGAC TGAGAGGAAG CGGCAAGGCT ACATCCATGA ACTCATTGTC
    ACGGAGGAGA ACTACGTGAA CGACTTGCAG CTGGTCACAG AGATCTTTCA GAAACCCCTG
    ACGGAGTCTG AGCTGCTGAC AGAAAAAGAG GTTGCTATGA TTTTTGTTAA CTGGAAGGAG
    CTGATCATGT GTAATATCAA ACTGCTGAAA GCGCTGAGAG TCCGCAAGAA GATGTCTGGG
    GAGAAGATGC CGGTGAAGAT GATTGGCGAC ATCCTGAGCG CCCAGCTGCC GCACATGCAG
    CCTTACATCC GCTTCTGCAG CTGCCAGCTC AATGGGGCTG CCCTCATCCA GCAGAAGACG
    GACGAGGCTC CAGACTTCAA GGAGTTCGTC AAAAGACTGG CAATGGACCC TCGGTGCAAA
    GGAATGCCTC TGTCCAGCTT TATACTGAAG CCTATGCAGC GTGTCACAAG ATACCCGCTG
    ATCATTAAAA ACATCCTGGA AAACACTCCC GAGAACCATC CAGACCACAG CCACCTGAAG
    CATGCCCTGG AAAAGGCGGA GGAGCTATGC TCCCAGGTGA ACGAGGGAGT TCGAGAGAAG
    GAGAACTCAG ACCGGCTGGA GTGGATCCAA GCCCACGTGC AGTGTGAAGG CCTTTCTGAG
    CAACTGGTGT TCAATTCAGT GACCAACTGC TTGGGACCAC GCAAGTTTCT GCACAGCGGG
    AAGCTCTACA AGGCCAAGAG CAACAAAGAA CTGTATGGCT TCCTCTTCAA CGACTTCCTC
    CTGCTGACCC AAATCACAAA GCCCTTAGGC TCTTCCGGCA CCGACAAAGT CTTCAGCCCC
    AAATCTAACC TTCAGTATAA AATGTACAAA ACGCCCATTT TCTTAAATGA GGTTCTAGTA
    AAATTGCCCA CGGACCCTTC TGGAGATGAG CCTATCTTCC ACATTTCCCA CATCGACCGG
    GTCTACACCC TCCGAGCAGA GAGCATAAAT GAGAGGACTG CCTGGGTGCA GAAAATCAAG
    GCGGCGTCTG AGCTCTACAT AGAGACGGAG AAAAAGAAGC GAGAGAAGGC GTACCTGGTC
    CGTTCCCAGC GGGCGACCGG TATTGGAAGG TTGATGGTGA ACGTGGTAGA AGGCATTGAG
    CTGAAGCCCT GTCGGTCACA TGGAAAGAGC AACCCGTACT GTGAGGTGAC CATGGGCTCT
    CAGTGCCACA TCACCAAGAC AATCCAGGAC ACGCTAAACC CCAAGTGGAA TTCTAACTGC
    CAGTTCTTCA TCAGAGACCT GGAGCAGGAG GTTCTCTGCA TCACAGTGTT TGAGAGGGAC
    CAGTTCTCGC CTGATGATTT TTTGGGTCGG ACAGAGATCC GAGTGGCCGA CATCAAGAAA
    GACCAGGGCT CCAAGGGGCC GGTTACGAAG TGTCTCCTGC TGCATGAGGT CCCCACGGGA
    GAGATTGTGG TCCGCCTTGA CCTGCAGTTG TTTGATGAGC CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_034717.2
    CDS262..5406
    Translation

    Target ORF information:

    RefSeq Version NM_010587.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_010587.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCCACACC TTTCGGTGGT AGCCTGGATG TCTGGGCCAT AACTGTGGAG 
    GAAAGGGCCA AGCATGACCA GCAGTTCCTT AGCCTGAAGC CGATAGCGGG ATTTATTACT
    GGTGATCAAG CGAGGAACTT TTTTTTCCAA TCTGGGTTAC CTCAGCCTGT CTTAGCACAA
    ATATGGGCGC TAGCGGACAT GAATAACGAT GGAAGGATGG ATCAAGTGGA ATTTTCCATA
    GCCATGAAGC TTATCAAACT GAAGCTACAA GGATATCAGC TCCCCTCCAC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT GGCTATTTCC AGTGCACCAG CATTTGGTAT AGGAGGGATT
    GCTAGCATGC CACCACTCAC AGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCAGTTGTT
    GGAATGTCTC CACCCTTAGT ATCTTCTGTC CCTCCAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCTC CCGTCATACA GCCTCTGCCT GCGTTTGCGC ATCCTGCAGC CACATTGCCA
    AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAATCAT TCGATGTCGC CAGCGCCCCT CCAGCAGCAG AATGGGCTGT GCCTCAGTCA
    TCAAGGCTGA AATACAGGCA GTTATTCAAC AGCCACGACA AAACTATGAG TGGACACTTA
    ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACTGCAGA AGAATTTATC
    CTAGCTATGC ACCTAATTGA TGTTGCCATG TCTGGTCAGC CACTGCCGCC CGTCCTGCCT
    CCAGAATACA TCCCTCCTTC CTTCAGAAGA GTTCGCTCCG GCAGTGGGAT GTCCGTCATA
    AGCTCTTCTT CTGTGGATCA GAGGCTGCCT GAGGAGCCGT CGTCAGAGGA TGAGCAGCAG
    CCAGAGAAGA AACTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC
    AGTGTGGAGC TGGAGAAGCG CCGCCAAGCG CTCTTGGAGC AGCAGCGCAA AGAGCAGGAG
    CGGTTGGCTC AGCTGGAGCG CGCCGAGCAG GAGAGGAAAG AGCGGGAGCG CCAGGAGCAG
    GAGCGCAAGC GGCAGCTGGA GCTGGAGAAG CAGCTGGAGA AGCAGCGGGA GCTGGAGCGG
    CAGCGAGAGG AGGAGAGGAG GAAGGAGATC GAGAGGCGCG AGGCCGCAAA ACGGGAACTG
    GAAAGGCAGC GACAACTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GAATCAGAGG
    AACAAGGAGC AGGAGGGCAC CGTGGTCCTG AAGGCAAGGA GGAAGACTCT GGAGTTTGAG
    TTAGAAGCTC TGAATGACAA AAAGCATCAG CTAGAAGGAA AACTTCAGGA TATCAGGTGT
    CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACGAACA AGTCTAGAGA GCTAAGAATT
    GCTGAAATCA CCCACTTACA GCAGCAGTTG CAGGAATCTC AGCAAATGCT TGGAAGACTT
    ATTCCAGAGA AACAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGTTTGCAT
    AGAGACTCGC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAGCTGGC CCGGCAGCAG
    CTCCGGGAGC AGCTGGACGA GGTGGAGAGA GAGACCAGGT CAAAGCTGCA GGAGATTGAT
    GTTTTCAACA ACCAGCTGAA GGAACTGAGA GAGATACATA GCAAACAGCA ACTCCAGAAG
    CAGAGGTCCC TGGAGGCAGC GCGACTGAAG CAGAAAGAGC AGGAGAGGAA GAGCCTGGAG
    TTAGAGAAGC AAAAGGAAGA CGCTCAGAGA CGAGTTCAGG AAAGGGACAA GCAATGGCTG
    GAGCATGTGC AGCAGGAGGA GCAGCCACGC CCCCGGAAAC CCCACGAGGA GGACAGACTG
    AAGAGGGAAG ACAGTGTCAG GAAGAAGGAG GCGGAAGAGA GAGCCAAGCC GGAAATGCAA
    GACAAGCAGA GTCGGCTTTT CCATCCGCAT CAGGAGCCAG CTAAGCTGGC CACCCAGGCA
    CCCTGGTCTA CCACAGAGAA AGGCCCGCTT ACCATTTCTG CACAGGAGAG TGTAAAAGTG
    GTATATTACC GAGCGCTGTA CCCCTTTGAA TCCAGAAGTC ACGATGAGAT CACCATCCAG
    CCAGGAGATA TAGTCATGGT GGATGAAAGC CAGACTGGAG AGCCAGGATG GCTTGGAGGA
    GAGCTGAAAG GGAAGACGGG ATGGTTCCCT GCAAACTATG CAGAAAAGAT TCCAGAAAAT
    GAGGTTCCCA CTCCAGCCAA ACCAGTGACC GATCTGACAT CTGCCCCTGC CCCCAAACTG
    GCTCTGCGTG AGACCCCTGC TCCTTTGCCA GTGACCTCTT CTGAGCCCTC CACAACCCCC
    AACAACTGGG CAGACTTCAG TTCCACGTGG CCCAGCAGCT CAAACGAGAA GCCAGAAACG
    GACAACTGGG ATACATGGGC GGCTCAGCCT TCTCTGACCG TACCTAGTGC TGGCCAGTTA
    CGGCAGAGAT CAGCCTTTAC CCCAGCCACA GCCACTGGCT CCTCCCCATC TCCCGTCCTG
    GGCCAGGGTG AAAAGGTGGA AGGGCTACAA GCGCAAGCCC TGTATCCCTG GAGAGCCAAA
    AAAGACAACC ACTTAAATTT TAACAAAAGT GACGTCATCA CCGTTCTGGA ACAGCAAGAC
    ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG AAGGGTTGGT TCCCCAAGTC TTACGTGAAA
    CTCATTTCAG GGCCCGTAAG GAAATCCACA AGCATCGATA CTGGCCCTAC TGAAAGTCCT
    GCTAGTCTAA AGAGAGTGGC TTCCCCGGCC GCCAAGCCAG CCATTCCCGG AGAAGAGTTT
    ATTGCCATGT ACACATACGA GAGTTCTGAG CAAGGAGATT TAACCTTTCA GCAAGGGGAT
    GTGATTGTGG TTACCAAGAA AGATGGTGAC TGGTGGACGG GAACGGTGGG CGACAAGTCC
    GGAGTCTTCC CTTCTAACTA TGTGAGGCTT AAAGATTCAG AGGGCTCTGG AACTGCTGGG
    AAAACAGGGA GTTTAGGAAA AAAACCTGAA ATTGCCCAGG TTATTGCTTC CTACGCTGCT
    ACTGGTCCCG AACAACTCAC CCTGGCTCCT GGGCAGCTGA TTCTGATCCG GAAAAAGAAC
    CCAGGTGGAT GGTGGGAAGG AGAACTGCAA GCTCGAGGGA AAAAGCGCCA GATAGGGTGG
    TTTCCAGCAA ATTATGTCAA ACTTCTAAGC CCCGGAACAA GCAAAATCAC CCCAACTGAG
    CTACCCAAGA CCGCAGTGCA GCCAGCAGTG TGCCAGGTGA TCGGGATGTA CGATTACACC
    GCCCAGAACG ATGACGAACT AGCCTTCAGC AAAGGCCAGA TCATCAACGT CCTCAACAAG
    GAGGACCCGG ACTGGTGGAA AGGAGAAGTC AGTGGGCAAG TTGGGCTCTT CCCATCCAAT
    TATGTAAAGC TGACCACAGA CATGGACCCC AGCCAGCAAT GGTGCTCAGA CCTGCATCTC
    TTAGATATGC TGACCCCGAC TGAGAGGAAG CGGCAAGGCT ACATCCATGA ACTCATTGTC
    ACGGAGGAGA ACTACGTGAA CGACTTGCAG CTGGTCACAG AGATCTTTCA GAAACCCCTG
    ACGGAGTCTG AGCTGCTGAC AGAAAAAGAG GTTGCTATGA TTTTTGTTAA CTGGAAGGAG
    CTGATCATGT GTAATATCAA ACTGCTGAAA GCGCTGAGAG TCCGCAAGAA GATGTCTGGG
    GAGAAGATGC CGGTGAAGAT GATTGGCGAC ATCCTGAGCG CCCAGCTGCC GCACATGCAG
    CCTTACATCC GCTTCTGCAG CTGCCAGCTC AATGGGGCTG CCCTCATCCA GCAGAAGACG
    GACGAGGCTC CAGACTTCAA GGAGTTCGTC AAAAGACTGG CAATGGACCC TCGGTGCAAA
    GGAATGCCTC TGTCCAGCTT TATACTGAAG CCTATGCAGC GTGTCACAAG ATACCCGCTG
    ATCATTAAAA ACATCCTGGA AAACACTCCC GAGAACCATC CAGACCACAG CCACCTGAAG
    CATGCCCTGG AAAAGGCGGA GGAGCTATGC TCCCAGGTGA ACGAGGGAGT TCGAGAGAAG
    GAGAACTCAG ACCGGCTGGA GTGGATCCAA GCCCACGTGC AGTGTGAAGG CCTTTCTGAG
    CAACTGGTGT TCAATTCAGT GACCAACTGC TTGGGACCAC GCAAGTTTCT GCACAGCGGG
    AAGCTCTACA AGGCCAAGAG CAACAAAGAA CTGTATGGCT TCCTCTTCAA CGACTTCCTC
    CTGCTGACCC AAATCACAAA GCCCTTAGGC TCTTCCGGCA CCGACAAAGT CTTCAGCCCC
    AAATCTAACC TTCAGTATAA AATGTACAAA ACGCCCATTT TCTTAAATGA GGTTCTAGTA
    AAATTGCCCA CGGACCCTTC TGGAGATGAG CCTATCTTCC ACATTTCCCA CATCGACCGG
    GTCTACACCC TCCGAGCAGA GAGCATAAAT GAGAGGACTG CCTGGGTGCA GAAAATCAAG
    GCGGCGTCTG AGCTCTACAT AGAGACGGAG AAAAAGAAGC GAGAGAAGGC GTACCTGGTC
    CGTTCCCAGC GGGCGACCGG TATTGGAAGG TTGATGGTGA ACGTGGTAGA AGGCATTGAG
    CTGAAGCCCT GTCGGTCACA TGGAAAGAGC AACCCGTACT GTGAGGTGAC CATGGGCTCT
    CAGTGCCACA TCACCAAGAC AATCCAGGAC ACGCTAAACC CCAAGTGGAA TTCTAACTGC
    CAGTTCTTCA TCAGAGACCT GGAGCAGGAG GTTCTCTGCA TCACAGTGTT TGAGAGGGAC
    CAGTTCTCGC CTGATGATTT TTTGGGTCGG ACAGAGATCC GAGTGGCCGA CATCAAGAAA
    GACCAGGGCT CCAAGGGGCC GGTTACGAAG TGTCTCCTGC TGCATGAGGT CCCCACGGGA
    GAGATTGTGG TCCGCCTTGA CCTGCAGTTG TTTGATGAGC CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13054
    Clone ID Related Accession (Same CDS sequence) NM_001110276.1
    Accession Version NM_001110276.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB519152.1 and BC066105.1. Transcript Variant: This variant (3) has multiple differences in the presence and absence of exons at its 3' end, compared to variant 1. These differences produce a unique 3' UTR and result in a shorter protein (isoform 3) with a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066105.1, AK160949.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCTCAGT TTCCCACACC TTTCGGTGGT AGCCTGGATG TCTGGGCCAT AACTGTGGAG 
    GAAAGGGCCA AGCATGACCA GCAGTTCCTT AGCCTGAAGC CGATAGCGGG ATTTATTACT
    GGTGATCAAG CGAGGAACTT TTTTTTCCAA TCTGGGTTAC CTCAGCCTGT CTTAGCACAA
    ATATGGGCGC TAGCGGACAT GAATAACGAT GGAAGGATGG ATCAAGTGGA ATTTTCCATA
    GCCATGAAGC TTATCAAACT GAAGCTACAA GGATATCAGC TCCCCTCCAC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT GGCTATTTCC AGTGCACCAG CATTTGGTAT AGGAGGGATT
    GCTAGCATGC CACCACTCAC AGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCAGTTGTT
    GGAATGTCTC CACCCTTAGT ATCTTCTGTC CCTCCAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCTC CCGTCATACA GCCTCTGCCT GCGTTTGCGC ATCCTGCAGC CACATTGCCA
    AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAATCAT TCGATGTCGC CAGCGCCCCT CCAGCAGCAG AATGGGCTGT GCCTCAGTCA
    TCAAGGCTGA AATACAGGCA GTTATTCAAC AGCCACGACA AAACTATGAG TGGACACTTA
    ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACTGCAGA AGAATTTATC
    CTAGCTATGC ACCTAATTGA TGTTGCCATG TCTGGTCAGC CACTGCCGCC CGTCCTGCCT
    CCAGAATACA TCCCTCCTTC CTTCAGAAGA GTTCGCTCCG GCAGTGGGAT GTCCGTCATA
    AGCTCTTCTT CTGTGGATCA GAGGCTGCCT GAGGAGCCGT CGTCAGAGGA TGAGCAGCAG
    CCAGAGAAGA AACTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC
    AGTGTGGAGC TGGAGAAGCG CCGCCAAGCG CTCTTGGAGC AGCAGCGCAA AGAGCAGGAG
    CGGTTGGCTC AGCTGGAGCG CGCCGAGCAG GAGAGGAAAG AGCGGGAGCG CCAGGAGCAG
    GAGCGCAAGC GGCAGCTGGA GCTGGAGAAG CAGCTGGAGA AGCAGCGGGA GCTGGAGCGG
    CAGCGAGAGG AGGAGAGGAG GAAGGAGATC GAGAGGCGCG AGGCCGCAAA ACGGGAACTG
    GAAAGGCAGC GACAACTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GAATCAGAGG
    AACAAGGAGC AGGAGGGCAC CGTGGTCCTG AAGGCAAGGA GGAAGACTCT GGAGTTTGAG
    TTAGAAGCTC TGAATGACAA AAAGCATCAG CTAGAAGGAA AACTTCAGGA TATCAGGTGT
    CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACGAACA AGTCTAGAGA GCTAAGAATT
    GCTGAAATCA CCCACTTACA GCAGCAGTTG CAGGAATCTC AGCAAATGCT TGGAAGACTT
    ATTCCAGAGA AACAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGTTTGCAT
    AGAGACTCGC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAGCTGGC CCGGCAGCAG
    CTCCGGGAGC AGCTGGACGA GGTGGAGAGA GAGACCAGGT CAAAGCTGCA GGAGATTGAT
    GTTTTCAACA ACCAGCTGAA GGTGACTCCT GGGTGCTGGG CCGCCTCCCC TTTGCTTGGC
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001103746.1
    CDS262..2127
    Translation

    Target ORF information:

    RefSeq Version NM_001110276.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001110276.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCTCAGT TTCCCACACC TTTCGGTGGT AGCCTGGATG TCTGGGCCAT AACTGTGGAG 
    GAAAGGGCCA AGCATGACCA GCAGTTCCTT AGCCTGAAGC CGATAGCGGG ATTTATTACT
    GGTGATCAAG CGAGGAACTT TTTTTTCCAA TCTGGGTTAC CTCAGCCTGT CTTAGCACAA
    ATATGGGCGC TAGCGGACAT GAATAACGAT GGAAGGATGG ATCAAGTGGA ATTTTCCATA
    GCCATGAAGC TTATCAAACT GAAGCTACAA GGATATCAGC TCCCCTCCAC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT GGCTATTTCC AGTGCACCAG CATTTGGTAT AGGAGGGATT
    GCTAGCATGC CACCACTCAC AGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCAGTTGTT
    GGAATGTCTC CACCCTTAGT ATCTTCTGTC CCTCCAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCTC CCGTCATACA GCCTCTGCCT GCGTTTGCGC ATCCTGCAGC CACATTGCCA
    AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAATCAT TCGATGTCGC CAGCGCCCCT CCAGCAGCAG AATGGGCTGT GCCTCAGTCA
    TCAAGGCTGA AATACAGGCA GTTATTCAAC AGCCACGACA AAACTATGAG TGGACACTTA
    ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACTGCAGA AGAATTTATC
    CTAGCTATGC ACCTAATTGA TGTTGCCATG TCTGGTCAGC CACTGCCGCC CGTCCTGCCT
    CCAGAATACA TCCCTCCTTC CTTCAGAAGA GTTCGCTCCG GCAGTGGGAT GTCCGTCATA
    AGCTCTTCTT CTGTGGATCA GAGGCTGCCT GAGGAGCCGT CGTCAGAGGA TGAGCAGCAG
    CCAGAGAAGA AACTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC
    AGTGTGGAGC TGGAGAAGCG CCGCCAAGCG CTCTTGGAGC AGCAGCGCAA AGAGCAGGAG
    CGGTTGGCTC AGCTGGAGCG CGCCGAGCAG GAGAGGAAAG AGCGGGAGCG CCAGGAGCAG
    GAGCGCAAGC GGCAGCTGGA GCTGGAGAAG CAGCTGGAGA AGCAGCGGGA GCTGGAGCGG
    CAGCGAGAGG AGGAGAGGAG GAAGGAGATC GAGAGGCGCG AGGCCGCAAA ACGGGAACTG
    GAAAGGCAGC GACAACTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GAATCAGAGG
    AACAAGGAGC AGGAGGGCAC CGTGGTCCTG AAGGCAAGGA GGAAGACTCT GGAGTTTGAG
    TTAGAAGCTC TGAATGACAA AAAGCATCAG CTAGAAGGAA AACTTCAGGA TATCAGGTGT
    CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACGAACA AGTCTAGAGA GCTAAGAATT
    GCTGAAATCA CCCACTTACA GCAGCAGTTG CAGGAATCTC AGCAAATGCT TGGAAGACTT
    ATTCCAGAGA AACAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGTTTGCAT
    AGAGACTCGC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAGCTGGC CCGGCAGCAG
    CTCCGGGAGC AGCTGGACGA GGTGGAGAGA GAGACCAGGT CAAAGCTGCA GGAGATTGAT
    GTTTTCAACA ACCAGCTGAA GGTGACTCCT GGGTGCTGGG CCGCCTCCCC TTTGCTTGGC
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13095
    Clone ID Related Accession (Same CDS sequence) NM_001110275.1
    Accession Version NM_001110275.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC062938.1 and AF132478.1. Transcript Variant: This variant (2) has multiple differences in the presence and absence of exons at its 3' end, compared to variant 1. These differences produce a unique 3' UTR and result in a protein (isoform 2) that is shorter at the C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCAGT TTCCCACACC TTTCGGTGGT AGCCTGGATG TCTGGGCCAT AACTGTGGAG 
    GAAAGGGCCA AGCATGACCA GCAGTTCCTT AGCCTGAAGC CGATAGCGGG ATTTATTACT
    GGTGATCAAG CGAGGAACTT TTTTTTCCAA TCTGGGTTAC CTCAGCCTGT CTTAGCACAA
    ATATGGGCGC TAGCGGACAT GAATAACGAT GGAAGGATGG ATCAAGTGGA ATTTTCCATA
    GCCATGAAGC TTATCAAACT GAAGCTACAA GGATATCAGC TCCCCTCCAC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT GGCTATTTCC AGTGCACCAG CATTTGGTAT AGGAGGGATT
    GCTAGCATGC CACCACTCAC AGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCAGTTGTT
    GGAATGTCTC CACCCTTAGT ATCTTCTGTC CCTCCAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCTC CCGTCATACA GCCTCTGCCT GCGTTTGCGC ATCCTGCAGC CACATTGCCA
    AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAATCAT TCGATGTCGC CAGCGCCCCT CCAGCAGCAG AATGGGCTGT GCCTCAGTCA
    TCAAGGCTGA AATACAGGCA GTTATTCAAC AGCCACGACA AAACTATGAG TGGACACTTA
    ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACTGCAGA AGAATTTATC
    CTAGCTATGC ACCTAATTGA TGTTGCCATG TCTGGTCAGC CACTGCCGCC CGTCCTGCCT
    CCAGAATACA TCCCTCCTTC CTTCAGAAGA GTTCGCTCCG GCAGTGGGAT GTCCGTCATA
    AGCTCTTCTT CTGTGGATCA GAGGCTGCCT GAGGAGCCGT CGTCAGAGGA TGAGCAGCAG
    CCAGAGAAGA AACTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC
    AGTGTGGAGC TGGAGAAGCG CCGCCAAGCG CTCTTGGAGC AGCAGCGCAA AGAGCAGGAG
    CGGTTGGCTC AGCTGGAGCG CGCCGAGCAG GAGAGGAAAG AGCGGGAGCG CCAGGAGCAG
    GAGCGCAAGC GGCAGCTGGA GCTGGAGAAG CAGCTGGAGA AGCAGCGGGA GCTGGAGCGG
    CAGCGAGAGG AGGAGAGGAG GAAGGAGATC GAGAGGCGCG AGGCCGCAAA ACGGGAACTG
    GAAAGGCAGC GACAACTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GAATCAGAGG
    AACAAGGAGC AGGAGGGCAC CGTGGTCCTG AAGGCAAGGA GGAAGACTCT GGAGTTTGAG
    TTAGAAGCTC TGAATGACAA AAAGCATCAG CTAGAAGGAA AACTTCAGGA TATCAGGTGT
    CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACGAACA AGTCTAGAGA GCTAAGAATT
    GCTGAAATCA CCCACTTACA GCAGCAGTTG CAGGAATCTC AGCAAATGCT TGGAAGACTT
    ATTCCAGAGA AACAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGTTTGCAT
    AGAGACTCGC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAGCTGGC CCGGCAGCAG
    CTCCGGGAGC AGCTGGACGA GGTGGAGAGA GAGACCAGGT CAAAGCTGCA GGAGATTGAT
    GTTTTCAACA ACCAGCTGAA GGAACTGAGA GAGATACATA GCAAACAGCA ACTCCAGAAG
    CAGAGGTCCC TGGAGGCAGC GCGACTGAAG CAGAAAGAGC AGGAGAGGAA GAGCCTGGAG
    TTAGAGAAGC AAAAGGAAGA CGCTCAGAGA CGAGTTCAGG AAAGGGACAA GCAATGGCTG
    GAGCATGTGC AGCAGGAGGA GCAGCCACGC CCCCGGAAAC CCCACGAGGA GGACAGACTG
    AAGAGGGAAG ACAGTGTCAG GAAGAAGGAG GCGGAAGAGA GAGCCAAGCC GGAAATGCAA
    GACAAGCAGA GTCGGCTTTT CCATCCGCAT CAGGAGCCAG CTAAGCTGGC CACCCAGGCA
    CCCTGGTCTA CCACAGAGAA AGGCCCGCTT ACCATTTCTG CACAGGAGAG TGTAAAAGTG
    GTATATTACC GAGCGCTGTA CCCCTTTGAA TCCAGAAGTC ACGATGAGAT CACCATCCAG
    CCAGGAGATA TAGTCATGGT GGATGAAAGC CAGACTGGAG AGCCAGGATG GCTTGGAGGA
    GAGCTGAAAG GGAAGACGGG ATGGTTCCCT GCAAACTATG CAGAAAAGAT TCCAGAAAAT
    GAGGTTCCCA CTCCAGCCAA ACCAGTGACC GATCTGACAT CTGCCCCTGC CCCCAAACTG
    GCTCTGCGTG AGACCCCTGC TCCTTTGCCA GTGACCTCTT CTGAGCCCTC CACAACCCCC
    AACAACTGGG CAGACTTCAG TTCCACGTGG CCCAGCAGCT CAAACGAGAA GCCAGAAACG
    GACAACTGGG ATACATGGGC GGCTCAGCCT TCTCTGACCG TACCTAGTGC TGGCCAGTTA
    CGGCAGAGAT CAGCCTTTAC CCCAGCCACA GCCACTGGCT CCTCCCCATC TCCCGTCCTG
    GGCCAGGGTG AAAAGGTGGA AGGGCTACAA GCGCAAGCCC TGTATCCCTG GAGAGCCAAA
    AAAGACAACC ACTTAAATTT TAACAAAAGT GACGTCATCA CCGTTCTGGA ACAGCAAGAC
    ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG AAGGGTTGGT TCCCCAAGTC TTACGTGAAA
    CTCATTTCAG GGCCCGTAAG GAAATCCACA AGCATCGATA CTGGCCCTAC TGAAAGTCCT
    GCTAGTCTAA AGAGAGTGGC TTCCCCGGCC GCCAAGCCAG CCATTCCCGG AGAAGAGTTT
    ATTGCCATGT ACACATACGA GAGTTCTGAG CAAGGAGATT TAACCTTTCA GCAAGGGGAT
    GTGATTGTGG TTACCAAGAA AGATGGTGAC TGGTGGACGG GAACGGTGGG CGACAAGTCC
    GGAGTCTTCC CTTCTAACTA TGTGAGGCTT AAAGATTCAG AGGGCTCTGG AACTGCTGGG
    AAAACAGGGA GTTTAGGAAA AAAACCTGAA ATTGCCCAGG TTATTGCTTC CTACGCTGCT
    ACTGGTCCCG AACAACTCAC CCTGGCTCCT GGGCAGCTGA TTCTGATCCG GAAAAAGAAC
    CCAGGTGGAT GGTGGGAAGG AGAACTGCAA GCTCGAGGGA AAAAGCGCCA GATAGGGTGG
    TTTCCAGCAA ATTATGTCAA ACTTCTAAGC CCCGGAACAA GCAAAATCAC CCCAACTGAG
    CTACCCAAGA CCGCAGTGCA GCCAGCAGTG TGCCAGGTGA TCGGGATGTA CGATTACACC
    GCCCAGAACG ATGACGAACT AGCCTTCAGC AAAGGCCAGA TCATCAACGT CCTCAACAAG
    GAGGACCCGG ACTGGTGGAA AGGAGAAGTC AGTGGGCAAG TTGGGCTCTT CCCATCCAAT
    TATGTAAAGC TGACCACAGA CATGGACCCC AGCCAGCAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001103745.1
    CDS262..3903
    Translation

    Target ORF information:

    RefSeq Version NM_001110275.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001110275.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCAGT TTCCCACACC TTTCGGTGGT AGCCTGGATG TCTGGGCCAT AACTGTGGAG 
    GAAAGGGCCA AGCATGACCA GCAGTTCCTT AGCCTGAAGC CGATAGCGGG ATTTATTACT
    GGTGATCAAG CGAGGAACTT TTTTTTCCAA TCTGGGTTAC CTCAGCCTGT CTTAGCACAA
    ATATGGGCGC TAGCGGACAT GAATAACGAT GGAAGGATGG ATCAAGTGGA ATTTTCCATA
    GCCATGAAGC TTATCAAACT GAAGCTACAA GGATATCAGC TCCCCTCCAC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT GGCTATTTCC AGTGCACCAG CATTTGGTAT AGGAGGGATT
    GCTAGCATGC CACCACTCAC AGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCAGTTGTT
    GGAATGTCTC CACCCTTAGT ATCTTCTGTC CCTCCAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCTC CCGTCATACA GCCTCTGCCT GCGTTTGCGC ATCCTGCAGC CACATTGCCA
    AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAATCAT TCGATGTCGC CAGCGCCCCT CCAGCAGCAG AATGGGCTGT GCCTCAGTCA
    TCAAGGCTGA AATACAGGCA GTTATTCAAC AGCCACGACA AAACTATGAG TGGACACTTA
    ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACTGCAGA AGAATTTATC
    CTAGCTATGC ACCTAATTGA TGTTGCCATG TCTGGTCAGC CACTGCCGCC CGTCCTGCCT
    CCAGAATACA TCCCTCCTTC CTTCAGAAGA GTTCGCTCCG GCAGTGGGAT GTCCGTCATA
    AGCTCTTCTT CTGTGGATCA GAGGCTGCCT GAGGAGCCGT CGTCAGAGGA TGAGCAGCAG
    CCAGAGAAGA AACTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC
    AGTGTGGAGC TGGAGAAGCG CCGCCAAGCG CTCTTGGAGC AGCAGCGCAA AGAGCAGGAG
    CGGTTGGCTC AGCTGGAGCG CGCCGAGCAG GAGAGGAAAG AGCGGGAGCG CCAGGAGCAG
    GAGCGCAAGC GGCAGCTGGA GCTGGAGAAG CAGCTGGAGA AGCAGCGGGA GCTGGAGCGG
    CAGCGAGAGG AGGAGAGGAG GAAGGAGATC GAGAGGCGCG AGGCCGCAAA ACGGGAACTG
    GAAAGGCAGC GACAACTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GAATCAGAGG
    AACAAGGAGC AGGAGGGCAC CGTGGTCCTG AAGGCAAGGA GGAAGACTCT GGAGTTTGAG
    TTAGAAGCTC TGAATGACAA AAAGCATCAG CTAGAAGGAA AACTTCAGGA TATCAGGTGT
    CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACGAACA AGTCTAGAGA GCTAAGAATT
    GCTGAAATCA CCCACTTACA GCAGCAGTTG CAGGAATCTC AGCAAATGCT TGGAAGACTT
    ATTCCAGAGA AACAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGTTTGCAT
    AGAGACTCGC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAGCTGGC CCGGCAGCAG
    CTCCGGGAGC AGCTGGACGA GGTGGAGAGA GAGACCAGGT CAAAGCTGCA GGAGATTGAT
    GTTTTCAACA ACCAGCTGAA GGAACTGAGA GAGATACATA GCAAACAGCA ACTCCAGAAG
    CAGAGGTCCC TGGAGGCAGC GCGACTGAAG CAGAAAGAGC AGGAGAGGAA GAGCCTGGAG
    TTAGAGAAGC AAAAGGAAGA CGCTCAGAGA CGAGTTCAGG AAAGGGACAA GCAATGGCTG
    GAGCATGTGC AGCAGGAGGA GCAGCCACGC CCCCGGAAAC CCCACGAGGA GGACAGACTG
    AAGAGGGAAG ACAGTGTCAG GAAGAAGGAG GCGGAAGAGA GAGCCAAGCC GGAAATGCAA
    GACAAGCAGA GTCGGCTTTT CCATCCGCAT CAGGAGCCAG CTAAGCTGGC CACCCAGGCA
    CCCTGGTCTA CCACAGAGAA AGGCCCGCTT ACCATTTCTG CACAGGAGAG TGTAAAAGTG
    GTATATTACC GAGCGCTGTA CCCCTTTGAA TCCAGAAGTC ACGATGAGAT CACCATCCAG
    CCAGGAGATA TAGTCATGGT GGATGAAAGC CAGACTGGAG AGCCAGGATG GCTTGGAGGA
    GAGCTGAAAG GGAAGACGGG ATGGTTCCCT GCAAACTATG CAGAAAAGAT TCCAGAAAAT
    GAGGTTCCCA CTCCAGCCAA ACCAGTGACC GATCTGACAT CTGCCCCTGC CCCCAAACTG
    GCTCTGCGTG AGACCCCTGC TCCTTTGCCA GTGACCTCTT CTGAGCCCTC CACAACCCCC
    AACAACTGGG CAGACTTCAG TTCCACGTGG CCCAGCAGCT CAAACGAGAA GCCAGAAACG
    GACAACTGGG ATACATGGGC GGCTCAGCCT TCTCTGACCG TACCTAGTGC TGGCCAGTTA
    CGGCAGAGAT CAGCCTTTAC CCCAGCCACA GCCACTGGCT CCTCCCCATC TCCCGTCCTG
    GGCCAGGGTG AAAAGGTGGA AGGGCTACAA GCGCAAGCCC TGTATCCCTG GAGAGCCAAA
    AAAGACAACC ACTTAAATTT TAACAAAAGT GACGTCATCA CCGTTCTGGA ACAGCAAGAC
    ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG AAGGGTTGGT TCCCCAAGTC TTACGTGAAA
    CTCATTTCAG GGCCCGTAAG GAAATCCACA AGCATCGATA CTGGCCCTAC TGAAAGTCCT
    GCTAGTCTAA AGAGAGTGGC TTCCCCGGCC GCCAAGCCAG CCATTCCCGG AGAAGAGTTT
    ATTGCCATGT ACACATACGA GAGTTCTGAG CAAGGAGATT TAACCTTTCA GCAAGGGGAT
    GTGATTGTGG TTACCAAGAA AGATGGTGAC TGGTGGACGG GAACGGTGGG CGACAAGTCC
    GGAGTCTTCC CTTCTAACTA TGTGAGGCTT AAAGATTCAG AGGGCTCTGG AACTGCTGGG
    AAAACAGGGA GTTTAGGAAA AAAACCTGAA ATTGCCCAGG TTATTGCTTC CTACGCTGCT
    ACTGGTCCCG AACAACTCAC CCTGGCTCCT GGGCAGCTGA TTCTGATCCG GAAAAAGAAC
    CCAGGTGGAT GGTGGGAAGG AGAACTGCAA GCTCGAGGGA AAAAGCGCCA GATAGGGTGG
    TTTCCAGCAA ATTATGTCAA ACTTCTAAGC CCCGGAACAA GCAAAATCAC CCCAACTGAG
    CTACCCAAGA CCGCAGTGCA GCCAGCAGTG TGCCAGGTGA TCGGGATGTA CGATTACACC
    GCCCAGAACG ATGACGAACT AGCCTTCAGC AAAGGCCAGA TCATCAACGT CCTCAACAAG
    GAGGACCCGG ACTGGTGGAA AGGAGAAGTC AGTGGGCAAG TTGGGCTCTT CCCATCCAAT
    TATGTAAAGC TGACCACAGA CATGGACCCC AGCCAGCAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52683
    Clone ID Related Accession (Same CDS sequence) XM_006522931.3 , XM_006522931.2
    Accession Version XM_006522931.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5232bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGGGTGAAA AGGTGGAAGG GCTACAAGCG
    CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA GACAACCACT TAAATTTTAA CAAAAGTGAC
    GTCATCACCG TTCTGGAACA GCAAGACATG TGGTGGTTTG GAGAAGTTCA AGGTCAGAAG
    GGTTGGTTCC CCAAGTCTTA CGTGAAACTC ATTTCAGGGC CCGTAAGGAA ATCCACAAGC
    ATCGATACTG GCCCTACTGA AAGTCCTGCT AGTCTAAAGA GAGTGGCTTC CCCGGCCGCC
    AAGCCAGCCA TTCCCGGAGA AGAGTTTATT GCCATGTACA CATACGAGAG TTCTGAGCAA
    GGAGATTTAA CCTTTCAGCA AGGGGATGTG ATTGTGGTTA CCAAGAAAGA TGGTGACTGG
    TGGACGGGAA CGGTGGGCGA CAAGTCCGGA GTCTTCCCTT CTAACTATGT GAGGCTTAAA
    GATTCAGAGG GCTCTGGAAC TGCTGGGAAA ACAGGGAGTT TAGGAAAAAA ACCTGAAATT
    GCCCAGGTTA TTGCTTCCTA CGCTGCTACT GGTCCCGAAC AACTCACCCT GGCTCCTGGG
    CAGCTGATTC TGATCCGGAA AAAGAACCCA GGTGGATGGT GGGAAGGAGA ACTGCAAGCT
    CGAGGGAAAA AGCGCCAGAT AGGGTGGTTT CCAGCAAATT ATGTCAAACT TCTAAGCCCC
    GGAACAAGCA AAATCACCCC AACTGAGCTA CCCAAGACCG CAGTGCAGCC AGCAGTGTGC
    CAGGTGATCG GGATGTACGA TTACACCGCC CAGAACGATG ACGAACTAGC CTTCAGCAAA
    GGCCAGATCA TCAACGTCCT CAACAAGGAG GACCCGGACT GGTGGAAAGG AGAAGTCAGT
    GGGCAAGTTG GGCTCTTCCC ATCCAATTAT GTAAAGCTGA CCACAGACAT GGACCCCAGC
    CAGCAATGGT GCTCAGACCT GCATCTCTTA GATATGCTGA CCCCGACTGA GAGGAAGCGG
    CAAGGCTACA TCCATGAACT CATTGTCACG GAGGAGAACT ACGTGAACGA CTTGCAGCTG
    GTCACAGAGA TCTTTCAGAA ACCCCTGACG GAGTCTGAGC TGCTGACAGA AAAAGAGGTT
    GCTATGATTT TTGTTAACTG GAAGGAGCTG ATCATGTGTA ATATCAAACT GCTGAAAGCG
    CTGAGAGTCC GCAAGAAGAT GTCTGGGGAG AAGATGCCGG TGAAGATGAT TGGCGACATC
    CTGAGCGCCC AGCTGCCGCA CATGCAGCCT TACATCCGCT TCTGCAGCTG CCAGCTCAAT
    GGGGCTGCCC TCATCCAGCA GAAGACGGAC GAGGCTCCAG ACTTCAAGGA GTTCGTCAAA
    AGACTGGCAA TGGACCCTCG GTGCAAAGGA ATGCCTCTGT CCAGCTTTAT ACTGAAGCCT
    ATGCAGCGTG TCACAAGATA CCCGCTGATC ATTAAAAACA TCCTGGAAAA CACTCCCGAG
    AACCATCCAG ACCACAGCCA CCTGAAGCAT GCCCTGGAAA AGGCGGAGGA GCTATGCTCC
    CAGGTGAACG AGGGAGTTCG AGAGAAGGAG AACTCAGACC GGCTGGAGTG GATCCAAGCC
    CACGTGCAGT GTGAAGGCCT TTCTGAGCAA CTGGTGTTCA ATTCAGTGAC CAACTGCTTG
    GGACCACGCA AGTTTCTGCA CAGCGGGAAG CTCTACAAGG CCAAGAGCAA CAAAGAACTG
    TATGGCTTCC TCTTCAACGA CTTCCTCCTG CTGACCCAAA TCACAAAGCC CTTAGGCTCT
    TCCGGCACCG ACAAAGTCTT CAGCCCCAAA TCTAACCTTC AGTATAAAAT GTACAAAACG
    CCCATTTTCT TAAATGAGGT TCTAGTAAAA TTGCCCACGG ACCCTTCTGG AGATGAGCCT
    ATCTTCCACA TTTCCCACAT CGACCGGGTC TACACCCTCC GAGCAGAGAG CATAAATGAG
    AGGACTGCCT GGGTGCAGAA AATCAAGGCG GCGTCTGAGC TCTACATAGA GACGGAGAAA
    AAGAAGCGAG AGAAGGCGTA CCTGGTCCGT TCCCAGCGGG CGACCGGTAT TGGAAGGTTG
    ATGGTGAACG TGGTAGAAGG CATTGAGCTG AAGCCCTGTC GGTCACATGG AAAGAGCAAC
    CCGTACTGTG AGGTGACCAT GGGCTCTCAG TGCCACATCA CCAAGACAAT CCAGGACACG
    CTAAACCCCA AGTGGAATTC TAACTGCCAG TTCTTCATCA GAGACCTGGA GCAGGAGGTT
    CTCTGCATCA CAGTGTTTGA GAGGGACCAG TTCTCGCCTG ATGATTTTTT GGGTCGGACA
    GAGATCCGAG TGGCCGACAT CAAGAAAGAC CAGGGCTCCA AGGGGCCGGT TACGAAGTGT
    CTCCTGCTGC ATGAGGTCCC CACGGGAGAG ATTGTGGTCC GCCTTGACCT GCAGTTGTTT
    GATGAGCCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522994.1
    CDS423..5654
    Translation

    Target ORF information:

    RefSeq Version XM_006522931.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522931.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGGGTGAAA AGGTGGAAGG GCTACAAGCG
    CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA GACAACCACT TAAATTTTAA CAAAAGTGAC
    GTCATCACCG TTCTGGAACA GCAAGACATG TGGTGGTTTG GAGAAGTTCA AGGTCAGAAG
    GGTTGGTTCC CCAAGTCTTA CGTGAAACTC ATTTCAGGGC CCGTAAGGAA ATCCACAAGC
    ATCGATACTG GCCCTACTGA AAGTCCTGCT AGTCTAAAGA GAGTGGCTTC CCCGGCCGCC
    AAGCCAGCCA TTCCCGGAGA AGAGTTTATT GCCATGTACA CATACGAGAG TTCTGAGCAA
    GGAGATTTAA CCTTTCAGCA AGGGGATGTG ATTGTGGTTA CCAAGAAAGA TGGTGACTGG
    TGGACGGGAA CGGTGGGCGA CAAGTCCGGA GTCTTCCCTT CTAACTATGT GAGGCTTAAA
    GATTCAGAGG GCTCTGGAAC TGCTGGGAAA ACAGGGAGTT TAGGAAAAAA ACCTGAAATT
    GCCCAGGTTA TTGCTTCCTA CGCTGCTACT GGTCCCGAAC AACTCACCCT GGCTCCTGGG
    CAGCTGATTC TGATCCGGAA AAAGAACCCA GGTGGATGGT GGGAAGGAGA ACTGCAAGCT
    CGAGGGAAAA AGCGCCAGAT AGGGTGGTTT CCAGCAAATT ATGTCAAACT TCTAAGCCCC
    GGAACAAGCA AAATCACCCC AACTGAGCTA CCCAAGACCG CAGTGCAGCC AGCAGTGTGC
    CAGGTGATCG GGATGTACGA TTACACCGCC CAGAACGATG ACGAACTAGC CTTCAGCAAA
    GGCCAGATCA TCAACGTCCT CAACAAGGAG GACCCGGACT GGTGGAAAGG AGAAGTCAGT
    GGGCAAGTTG GGCTCTTCCC ATCCAATTAT GTAAAGCTGA CCACAGACAT GGACCCCAGC
    CAGCAATGGT GCTCAGACCT GCATCTCTTA GATATGCTGA CCCCGACTGA GAGGAAGCGG
    CAAGGCTACA TCCATGAACT CATTGTCACG GAGGAGAACT ACGTGAACGA CTTGCAGCTG
    GTCACAGAGA TCTTTCAGAA ACCCCTGACG GAGTCTGAGC TGCTGACAGA AAAAGAGGTT
    GCTATGATTT TTGTTAACTG GAAGGAGCTG ATCATGTGTA ATATCAAACT GCTGAAAGCG
    CTGAGAGTCC GCAAGAAGAT GTCTGGGGAG AAGATGCCGG TGAAGATGAT TGGCGACATC
    CTGAGCGCCC AGCTGCCGCA CATGCAGCCT TACATCCGCT TCTGCAGCTG CCAGCTCAAT
    GGGGCTGCCC TCATCCAGCA GAAGACGGAC GAGGCTCCAG ACTTCAAGGA GTTCGTCAAA
    AGACTGGCAA TGGACCCTCG GTGCAAAGGA ATGCCTCTGT CCAGCTTTAT ACTGAAGCCT
    ATGCAGCGTG TCACAAGATA CCCGCTGATC ATTAAAAACA TCCTGGAAAA CACTCCCGAG
    AACCATCCAG ACCACAGCCA CCTGAAGCAT GCCCTGGAAA AGGCGGAGGA GCTATGCTCC
    CAGGTGAACG AGGGAGTTCG AGAGAAGGAG AACTCAGACC GGCTGGAGTG GATCCAAGCC
    CACGTGCAGT GTGAAGGCCT TTCTGAGCAA CTGGTGTTCA ATTCAGTGAC CAACTGCTTG
    GGACCACGCA AGTTTCTGCA CAGCGGGAAG CTCTACAAGG CCAAGAGCAA CAAAGAACTG
    TATGGCTTCC TCTTCAACGA CTTCCTCCTG CTGACCCAAA TCACAAAGCC CTTAGGCTCT
    TCCGGCACCG ACAAAGTCTT CAGCCCCAAA TCTAACCTTC AGTATAAAAT GTACAAAACG
    CCCATTTTCT TAAATGAGGT TCTAGTAAAA TTGCCCACGG ACCCTTCTGG AGATGAGCCT
    ATCTTCCACA TTTCCCACAT CGACCGGGTC TACACCCTCC GAGCAGAGAG CATAAATGAG
    AGGACTGCCT GGGTGCAGAA AATCAAGGCG GCGTCTGAGC TCTACATAGA GACGGAGAAA
    AAGAAGCGAG AGAAGGCGTA CCTGGTCCGT TCCCAGCGGG CGACCGGTAT TGGAAGGTTG
    ATGGTGAACG TGGTAGAAGG CATTGAGCTG AAGCCCTGTC GGTCACATGG AAAGAGCAAC
    CCGTACTGTG AGGTGACCAT GGGCTCTCAG TGCCACATCA CCAAGACAAT CCAGGACACG
    CTAAACCCCA AGTGGAATTC TAACTGCCAG TTCTTCATCA GAGACCTGGA GCAGGAGGTT
    CTCTGCATCA CAGTGTTTGA GAGGGACCAG TTCTCGCCTG ATGATTTTTT GGGTCGGACA
    GAGATCCGAG TGGCCGACAT CAAGAAAGAC CAGGGCTCCA AGGGGCCGGT TACGAAGTGT
    CTCCTGCTGC ATGAGGTCCC CACGGGAGAG ATTGTGGTCC GCCTTGACCT GCAGTTGTTT
    GATGAGCCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52684
    Clone ID Related Accession (Same CDS sequence) XM_006522932.3 , XM_006522932.4
    Accession Version XM_006522932.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522932.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGACACA TTTTCACAGC TTTCTCCAAA CTCTGCGTCG ATCAGCAAGG TGAACGTAAT 
    AGAACCATGG CTCAGTTTCC CACACCTTTC GGTGGTAGCC TGGATGTCTG GGCCATAACT
    GTGGAGGAAA GGGCCAAGCA TGACCAGCAG TTCCTTAGCC TGAAGCCGAT AGCGGGATTT
    ATTACTGGTG ATCAAGCGAG GAACTTTTTT TTCCAATCTG GGTTACCTCA GCCTGTCTTA
    GCACAAATAT GGGCGCTAGC GGACATGAAT AACGATGGAA GGATGGATCA AGTGGAATTT
    TCCATAGCCA TGAAGCTTAT CAAACTGAAG CTACAAGGAT ATCAGCTCCC CTCCACACTT
    CCCCCTGTCA TGAAACAGCA ACCAGTGGCT ATTTCCAGTG CACCAGCATT TGGTATAGGA
    GGGATTGCTA GCATGCCACC ACTCACAGCT GTTGCTCCTG TGCCAATGGG CTCCATTCCA
    GTTGTTGGAA TGTCTCCACC CTTAGTATCT TCTGTCCCTC CAGCAGCAGT GCCTCCCCTG
    GCTAACGGGG CTCCTCCCGT CATACAGCCT CTGCCTGCGT TTGCGCATCC TGCAGCCACA
    TTGCCAAAGA GTTCTTCCTT CAGCAGATCT GGTCCAGGGT CACAATTAAA CACTAAGTTA
    CAGAAGGCAC AATCATTCGA TGTCGCCAGC GCCCCTCCAG CAGCAGAATG GGCTGTGCCT
    CAGTCATCAA GGCTGAAATA CAGGCAGTTA TTCAACAGCC ACGACAAAAC TATGAGTGGA
    CACTTAACAG GTCCCCAGGC AAGAACTATT CTCATGCAAT CAAGTTTACC CCAGGCTCAG
    CTGGCTTCAA TATGGAATCT TTCTGACATT GATCAAGATG GAAAACTCAC TGCAGAAGAA
    TTTATCCTAG CTATGCACCT AATTGATGTT GCCATGTCTG GTCAGCCACT GCCGCCCGTC
    CTGCCTCCAG AATACATCCC TCCTTCCTTC AGAAGAGTTC GCTCCGGCAG TGGGATGTCC
    GTCATAAGCT CTTCTTCTGT GGATCAGAGG CTGCCTGAGG AGCCGTCGTC AGAGGATGAG
    CAGCAGCCAG AGAAGAAACT GCCTGTGACA TTTGAAGATA AGAAGCGGGA GAACTTCGAG
    CGAGGCAGTG TGGAGCTGGA GAAGCGCCGC CAAGCGCTCT TGGAGCAGCA GCGCAAAGAG
    CAGGAGCGGT TGGCTCAGCT GGAGCGCGCC GAGCAGGAGA GGAAAGAGCG GGAGCGCCAG
    GAGCAGGAGC GCAAGCGGCA GCTGGAGCTG GAGAAGCAGC TGGAGAAGCA GCGGGAGCTG
    GAGCGGCAGC GAGAGGAGGA GAGGAGGAAG GAGATCGAGA GGCGCGAGGC CGCAAAACGG
    GAACTGGAAA GGCAGCGACA ACTTGAATGG GAACGGAACC GGAGACAGGA ACTCCTGAAT
    CAGAGGAACA AGGAGCAGGA GGGCACCGTG GTCCTGAAGG CAAGGAGGAA GACTCTGGAG
    TTTGAGTTAG AAGCTCTGAA TGACAAAAAG CATCAGCTAG AAGGAAAACT TCAGGATATC
    AGGTGTCGAC TGGCAACCCA GAGGCAAGAA ATTGAGAGCA CGAACAAGTC TAGAGAGCTA
    AGAATTGCTG AAATCACCCA CTTACAGCAG CAGTTGCAGG AATCTCAGCA AATGCTTGGA
    AGACTTATTC CAGAGAAACA GATACTCAGT GACCAGTTAA AACAAGTCCA GCAGAACAGT
    TTGCATAGAG ACTCGCTTCT TACCCTCAAA AGAGCCTTGG AAGCAAAGGA GCTGGCCCGG
    CAGCAGCTCC GGGAGCAGCT GGACGAGGTG GAGAGAGAGA CCAGGTCAAA GCTGCAGGAG
    ATTGATGTTT TCAACAACCA GCTGAAGGAA CTGAGAGAGA TACATAGCAA ACAGCAACTC
    CAGAAGCAGA GGTCCCTGGA GGCAGCGCGA CTGAAGCAGA AAGAGCAGGA GAGGAAGAGC
    CTGGAGTTAG AGAAGCAAAA GGAAGACGCT CAGAGACGAG TTCAGGAAAG GGACAAGCAA
    TGGCTGGAGC ATGTGCAGCA GGAGGAGCAG CCACGCCCCC GGAAACCCCA CGAGGAGGAC
    AGACTGAAGA GGGAAGACAG TGTCAGGAAG AAGGAGGCGG AAGAGAGAGC CAAGCCGGAA
    ATGCAAGACA AGCAGAGTCG GCTTTTCCAT CCGCATCAGG AGCCAGCTAA GCTGGCCACC
    CAGGCACCCT GGTCTACCAC AGAGAAAGGC CCGCTTACCA TTTCTGCACA GGAGAGTGTA
    AAAGTGGTAT ATTACCGAGC GCTGTACCCC TTTGAATCCA GAAGTCACGA TGAGATCACC
    ATCCAGCCAG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAGACTGGA
    GAGCCAGGAT GGCTTGGAGG AGAGCTGAAA GGGAAGACGG GATGGTTCCC TGCAAACTAT
    GCAGAAAAGA TTCCAGAAAA TGAGGTTCCC ACTCCAGCCA AACCAGTGAC CGATCTGACA
    TCTGCCCCTG CCCCCAAACT GGCTCTGCGT GAGACCCCTG CTCCTTTGCC AGTGACCTCT
    TCTGAGCCCT CCACAACCCC CAACAACTGG GCAGACTTCA GTTCCACGTG GCCCAGCAGC
    TCAAACGAGA AGCCAGAAAC GGACAACTGG GATACATGGG CGGCTCAGCC TTCTCTGACC
    GTACCTAGTG CTGGCCAGTT ACGGCAGAGA TCAGCCTTTA CCCCAGCCAC AGCCACTGGC
    TCCTCCCCAT CTCCCGTCCT GGGCCAGGGT GAAAAGGTGG AAGGGCTACA AGCGCAAGCC
    CTGTATCCCT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAG TGACGTCATC
    ACCGTTCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCGTAA GGAAATCCAC AAGCATCGAT
    ACTGGCCCTA CTGAAAGTCC TGCTAGTCTA AAGAGAGTGG CTTCCCCGGC CGCCAAGCCA
    GCCATTCCCG GAGAAGAGTT TATTGCCATG TACACATACG AGAGTTCTGA GCAAGGAGAT
    TTAACCTTTC AGCAAGGGGA TGTGATTGTG GTTACCAAGA AAGATGGTGA CTGGTGGACG
    GGAACGGTGG GCGACAAGTC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG
    GTTATTGCTT CCTACGCTGC TACTGGTCCC GAACAACTCA CCCTGGCTCC TGGGCAGCTG
    ATTCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAACTGCA AGCTCGAGGG
    AAAAAGCGCC AGATAGGGTG GTTTCCAGCA AATTATGTCA AACTTCTAAG CCCCGGAACA
    AGCAAAATCA CCCCAACTGA GCTACCCAAG ACCGCAGTGC AGCCAGCAGT GTGCCAGGTG
    ATCGGGATGT ACGATTACAC CGCCCAGAAC GATGACGAAC TAGCCTTCAG CAAAGGCCAG
    ATCATCAACG TCCTCAACAA GGAGGACCCG GACTGGTGGA AAGGAGAAGT CAGTGGGCAA
    GTTGGGCTCT TCCCATCCAA TTATGTAAAG CTGACCACAG ACATGGACCC CAGCCAGCAA
    TGGTGCTCAG ACCTGCATCT CTTAGATATG CTGACCCCGA CTGAGAGGAA GCGGCAAGGC
    TACATCCATG AACTCATTGT CACGGAGGAG AACTACGTGA ACGACTTGCA GCTGGTCACA
    GAGATCTTTC AGAAACCCCT GACGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG
    ATTTTTGTTA ACTGGAAGGA GCTGATCATG TGTAATATCA AACTGCTGAA AGCGCTGAGA
    GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCGGTGAAGA TGATTGGCGA CATCCTGAGC
    GCCCAGCTGC CGCACATGCA GCCTTACATC CGCTTCTGCA GCTGCCAGCT CAATGGGGCT
    GCCCTCATCC AGCAGAAGAC GGACGAGGCT CCAGACTTCA AGGAGTTCGT CAAAAGACTG
    GCAATGGACC CTCGGTGCAA AGGAATGCCT CTGTCCAGCT TTATACTGAA GCCTATGCAG
    CGTGTCACAA GATACCCGCT GATCATTAAA AACATCCTGG AAAACACTCC CGAGAACCAT
    CCAGACCACA GCCACCTGAA GCATGCCCTG GAAAAGGCGG AGGAGCTATG CTCCCAGGTG
    AACGAGGGAG TTCGAGAGAA GGAGAACTCA GACCGGCTGG AGTGGATCCA AGCCCACGTG
    CAGTGTGAAG GCCTTTCTGA GCAACTGGTG TTCAATTCAG TGACCAACTG CTTGGGACCA
    CGCAAGTTTC TGCACAGCGG GAAGCTCTAC AAGGCCAAGA GCAACAAAGA ACTGTATGGC
    TTCCTCTTCA ACGACTTCCT CCTGCTGACC CAAATCACAA AGCCCTTAGG CTCTTCCGGC
    ACCGACAAAG TCTTCAGCCC CAAATCTAAC CTTCAGTATA AAATGTACAA AACGCCCATT
    TTCTTAAATG AGGTTCTAGT AAAATTGCCC ACGGACCCTT CTGGAGATGA GCCTATCTTC
    CACATTTCCC ACATCGACCG GGTCTACACC CTCCGAGCAG AGAGCATAAA TGAGAGGACT
    GCCTGGGTGC AGAAAATCAA GGCGGCGTCT GAGCTCTACA TAGAGACGGA GAAAAAGAAG
    CGAGAGAAGG CGTACCTGGT CCGTTCCCAG CGGGCGACCG GTATTGGAAG GTTGATGGTG
    AACGTGGTAG AAGGCATTGA GCTGAAGCCC TGTCGGTCAC ATGGAAAGAG CAACCCGTAC
    TGTGAGGTGA CCATGGGCTC TCAGTGCCAC ATCACCAAGA CAATCCAGGA CACGCTAAAC
    CCCAAGTGGA ATTCTAACTG CCAGTTCTTC ATCAGAGACC TGGAGCAGGA GGTTCTCTGC
    ATCACAGTGT TTGAGAGGGA CCAGTTCTCG CCTGATGATT TTTTGGGTCG GACAGAGATC
    CGAGTGGCCG ACATCAAGAA AGACCAGGGC TCCAAGGGGC CGGTTACGAA GTGTCTCCTG
    CTGCATGAGG TCCCCACGGG AGAGATTGTG GTCCGCCTTG ACCTGCAGTT GTTTGATGAG
    CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522995.1
    CDS766..5991
    Translation

    Target ORF information:

    RefSeq Version XM_006522932.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522932.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGACACA TTTTCACAGC TTTCTCCAAA CTCTGCGTCG ATCAGCAAGG TGAACGTAAT 
    AGAACCATGG CTCAGTTTCC CACACCTTTC GGTGGTAGCC TGGATGTCTG GGCCATAACT
    GTGGAGGAAA GGGCCAAGCA TGACCAGCAG TTCCTTAGCC TGAAGCCGAT AGCGGGATTT
    ATTACTGGTG ATCAAGCGAG GAACTTTTTT TTCCAATCTG GGTTACCTCA GCCTGTCTTA
    GCACAAATAT GGGCGCTAGC GGACATGAAT AACGATGGAA GGATGGATCA AGTGGAATTT
    TCCATAGCCA TGAAGCTTAT CAAACTGAAG CTACAAGGAT ATCAGCTCCC CTCCACACTT
    CCCCCTGTCA TGAAACAGCA ACCAGTGGCT ATTTCCAGTG CACCAGCATT TGGTATAGGA
    GGGATTGCTA GCATGCCACC ACTCACAGCT GTTGCTCCTG TGCCAATGGG CTCCATTCCA
    GTTGTTGGAA TGTCTCCACC CTTAGTATCT TCTGTCCCTC CAGCAGCAGT GCCTCCCCTG
    GCTAACGGGG CTCCTCCCGT CATACAGCCT CTGCCTGCGT TTGCGCATCC TGCAGCCACA
    TTGCCAAAGA GTTCTTCCTT CAGCAGATCT GGTCCAGGGT CACAATTAAA CACTAAGTTA
    CAGAAGGCAC AATCATTCGA TGTCGCCAGC GCCCCTCCAG CAGCAGAATG GGCTGTGCCT
    CAGTCATCAA GGCTGAAATA CAGGCAGTTA TTCAACAGCC ACGACAAAAC TATGAGTGGA
    CACTTAACAG GTCCCCAGGC AAGAACTATT CTCATGCAAT CAAGTTTACC CCAGGCTCAG
    CTGGCTTCAA TATGGAATCT TTCTGACATT GATCAAGATG GAAAACTCAC TGCAGAAGAA
    TTTATCCTAG CTATGCACCT AATTGATGTT GCCATGTCTG GTCAGCCACT GCCGCCCGTC
    CTGCCTCCAG AATACATCCC TCCTTCCTTC AGAAGAGTTC GCTCCGGCAG TGGGATGTCC
    GTCATAAGCT CTTCTTCTGT GGATCAGAGG CTGCCTGAGG AGCCGTCGTC AGAGGATGAG
    CAGCAGCCAG AGAAGAAACT GCCTGTGACA TTTGAAGATA AGAAGCGGGA GAACTTCGAG
    CGAGGCAGTG TGGAGCTGGA GAAGCGCCGC CAAGCGCTCT TGGAGCAGCA GCGCAAAGAG
    CAGGAGCGGT TGGCTCAGCT GGAGCGCGCC GAGCAGGAGA GGAAAGAGCG GGAGCGCCAG
    GAGCAGGAGC GCAAGCGGCA GCTGGAGCTG GAGAAGCAGC TGGAGAAGCA GCGGGAGCTG
    GAGCGGCAGC GAGAGGAGGA GAGGAGGAAG GAGATCGAGA GGCGCGAGGC CGCAAAACGG
    GAACTGGAAA GGCAGCGACA ACTTGAATGG GAACGGAACC GGAGACAGGA ACTCCTGAAT
    CAGAGGAACA AGGAGCAGGA GGGCACCGTG GTCCTGAAGG CAAGGAGGAA GACTCTGGAG
    TTTGAGTTAG AAGCTCTGAA TGACAAAAAG CATCAGCTAG AAGGAAAACT TCAGGATATC
    AGGTGTCGAC TGGCAACCCA GAGGCAAGAA ATTGAGAGCA CGAACAAGTC TAGAGAGCTA
    AGAATTGCTG AAATCACCCA CTTACAGCAG CAGTTGCAGG AATCTCAGCA AATGCTTGGA
    AGACTTATTC CAGAGAAACA GATACTCAGT GACCAGTTAA AACAAGTCCA GCAGAACAGT
    TTGCATAGAG ACTCGCTTCT TACCCTCAAA AGAGCCTTGG AAGCAAAGGA GCTGGCCCGG
    CAGCAGCTCC GGGAGCAGCT GGACGAGGTG GAGAGAGAGA CCAGGTCAAA GCTGCAGGAG
    ATTGATGTTT TCAACAACCA GCTGAAGGAA CTGAGAGAGA TACATAGCAA ACAGCAACTC
    CAGAAGCAGA GGTCCCTGGA GGCAGCGCGA CTGAAGCAGA AAGAGCAGGA GAGGAAGAGC
    CTGGAGTTAG AGAAGCAAAA GGAAGACGCT CAGAGACGAG TTCAGGAAAG GGACAAGCAA
    TGGCTGGAGC ATGTGCAGCA GGAGGAGCAG CCACGCCCCC GGAAACCCCA CGAGGAGGAC
    AGACTGAAGA GGGAAGACAG TGTCAGGAAG AAGGAGGCGG AAGAGAGAGC CAAGCCGGAA
    ATGCAAGACA AGCAGAGTCG GCTTTTCCAT CCGCATCAGG AGCCAGCTAA GCTGGCCACC
    CAGGCACCCT GGTCTACCAC AGAGAAAGGC CCGCTTACCA TTTCTGCACA GGAGAGTGTA
    AAAGTGGTAT ATTACCGAGC GCTGTACCCC TTTGAATCCA GAAGTCACGA TGAGATCACC
    ATCCAGCCAG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAGACTGGA
    GAGCCAGGAT GGCTTGGAGG AGAGCTGAAA GGGAAGACGG GATGGTTCCC TGCAAACTAT
    GCAGAAAAGA TTCCAGAAAA TGAGGTTCCC ACTCCAGCCA AACCAGTGAC CGATCTGACA
    TCTGCCCCTG CCCCCAAACT GGCTCTGCGT GAGACCCCTG CTCCTTTGCC AGTGACCTCT
    TCTGAGCCCT CCACAACCCC CAACAACTGG GCAGACTTCA GTTCCACGTG GCCCAGCAGC
    TCAAACGAGA AGCCAGAAAC GGACAACTGG GATACATGGG CGGCTCAGCC TTCTCTGACC
    GTACCTAGTG CTGGCCAGTT ACGGCAGAGA TCAGCCTTTA CCCCAGCCAC AGCCACTGGC
    TCCTCCCCAT CTCCCGTCCT GGGCCAGGGT GAAAAGGTGG AAGGGCTACA AGCGCAAGCC
    CTGTATCCCT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAG TGACGTCATC
    ACCGTTCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCGTAA GGAAATCCAC AAGCATCGAT
    ACTGGCCCTA CTGAAAGTCC TGCTAGTCTA AAGAGAGTGG CTTCCCCGGC CGCCAAGCCA
    GCCATTCCCG GAGAAGAGTT TATTGCCATG TACACATACG AGAGTTCTGA GCAAGGAGAT
    TTAACCTTTC AGCAAGGGGA TGTGATTGTG GTTACCAAGA AAGATGGTGA CTGGTGGACG
    GGAACGGTGG GCGACAAGTC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG
    GTTATTGCTT CCTACGCTGC TACTGGTCCC GAACAACTCA CCCTGGCTCC TGGGCAGCTG
    ATTCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAACTGCA AGCTCGAGGG
    AAAAAGCGCC AGATAGGGTG GTTTCCAGCA AATTATGTCA AACTTCTAAG CCCCGGAACA
    AGCAAAATCA CCCCAACTGA GCTACCCAAG ACCGCAGTGC AGCCAGCAGT GTGCCAGGTG
    ATCGGGATGT ACGATTACAC CGCCCAGAAC GATGACGAAC TAGCCTTCAG CAAAGGCCAG
    ATCATCAACG TCCTCAACAA GGAGGACCCG GACTGGTGGA AAGGAGAAGT CAGTGGGCAA
    GTTGGGCTCT TCCCATCCAA TTATGTAAAG CTGACCACAG ACATGGACCC CAGCCAGCAA
    TGGTGCTCAG ACCTGCATCT CTTAGATATG CTGACCCCGA CTGAGAGGAA GCGGCAAGGC
    TACATCCATG AACTCATTGT CACGGAGGAG AACTACGTGA ACGACTTGCA GCTGGTCACA
    GAGATCTTTC AGAAACCCCT GACGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG
    ATTTTTGTTA ACTGGAAGGA GCTGATCATG TGTAATATCA AACTGCTGAA AGCGCTGAGA
    GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCGGTGAAGA TGATTGGCGA CATCCTGAGC
    GCCCAGCTGC CGCACATGCA GCCTTACATC CGCTTCTGCA GCTGCCAGCT CAATGGGGCT
    GCCCTCATCC AGCAGAAGAC GGACGAGGCT CCAGACTTCA AGGAGTTCGT CAAAAGACTG
    GCAATGGACC CTCGGTGCAA AGGAATGCCT CTGTCCAGCT TTATACTGAA GCCTATGCAG
    CGTGTCACAA GATACCCGCT GATCATTAAA AACATCCTGG AAAACACTCC CGAGAACCAT
    CCAGACCACA GCCACCTGAA GCATGCCCTG GAAAAGGCGG AGGAGCTATG CTCCCAGGTG
    AACGAGGGAG TTCGAGAGAA GGAGAACTCA GACCGGCTGG AGTGGATCCA AGCCCACGTG
    CAGTGTGAAG GCCTTTCTGA GCAACTGGTG TTCAATTCAG TGACCAACTG CTTGGGACCA
    CGCAAGTTTC TGCACAGCGG GAAGCTCTAC AAGGCCAAGA GCAACAAAGA ACTGTATGGC
    TTCCTCTTCA ACGACTTCCT CCTGCTGACC CAAATCACAA AGCCCTTAGG CTCTTCCGGC
    ACCGACAAAG TCTTCAGCCC CAAATCTAAC CTTCAGTATA AAATGTACAA AACGCCCATT
    TTCTTAAATG AGGTTCTAGT AAAATTGCCC ACGGACCCTT CTGGAGATGA GCCTATCTTC
    CACATTTCCC ACATCGACCG GGTCTACACC CTCCGAGCAG AGAGCATAAA TGAGAGGACT
    GCCTGGGTGC AGAAAATCAA GGCGGCGTCT GAGCTCTACA TAGAGACGGA GAAAAAGAAG
    CGAGAGAAGG CGTACCTGGT CCGTTCCCAG CGGGCGACCG GTATTGGAAG GTTGATGGTG
    AACGTGGTAG AAGGCATTGA GCTGAAGCCC TGTCGGTCAC ATGGAAAGAG CAACCCGTAC
    TGTGAGGTGA CCATGGGCTC TCAGTGCCAC ATCACCAAGA CAATCCAGGA CACGCTAAAC
    CCCAAGTGGA ATTCTAACTG CCAGTTCTTC ATCAGAGACC TGGAGCAGGA GGTTCTCTGC
    ATCACAGTGT TTGAGAGGGA CCAGTTCTCG CCTGATGATT TTTTGGGTCG GACAGAGATC
    CGAGTGGCCG ACATCAAGAA AGACCAGGGC TCCAAGGGGC CGGTTACGAA GTGTCTCCTG
    CTGCATGAGG TCCCCACGGG AGAGATTGTG GTCCGCCTTG ACCTGCAGTT GTTTGATGAG
    CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52685
    Clone ID Related Accession (Same CDS sequence) XM_006522933.2
    Accession Version XM_006522933.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTGGATGAAA GCCAGACTGG AGAGCCAGGA
    TGGCTTGGAG GAGAGCTGAA AGGGAAGACG GGATGGTTCC CTGCAAACTA TGCAGAAAAG
    ATTCCAGAAA ATGAGGTTCC CACTCCAGCC AAACCAGTGA CCGATCTGAC ATCTGCCCCT
    GCCCCCAAAC TGGCTCTGCG TGAGACCCCT GCTCCTTTGC CAGTGACCTC TTCTGAGCCC
    TCCACAACCC CCAACAACTG GGCAGACTTC AGTTCCACGT GGCCCAGCAG CTCAAACGAG
    AAGCCAGAAA CGGACAACTG GGATACATGG GCGGCTCAGC CTTCTCTGAC CGTACCTAGT
    GCTGGCCAGT TACGGCAGAG ATCAGCCTTT ACCCCAGCCA CAGCCACTGG CTCCTCCCCA
    TCTCCCGTCC TGGGCCAGGG TGAAAAGGTG GAAGGGCTAC AAGCGCAAGC CCTGTATCCC
    TGGAGAGCCA AAAAAGACAA CCACTTAAAT TTTAACAAAA GTGACGTCAT CACCGTTCTG
    GAACAGCAAG ACATGTGGTG GTTTGGAGAA GTTCAAGGTC AGAAGGGTTG GTTCCCCAAG
    TCTTACGTGA AACTCATTTC AGGGCCCGTA AGGAAATCCA CAAGCATCGA TACTGGCCCT
    ACTGAAAGTC CTGCTAGTCT AAAGAGAGTG GCTTCCCCGG CCGCCAAGCC AGCCATTCCC
    GGAGAAGAGT TTATTGCCAT GTACACATAC GAGAGTTCTG AGCAAGGAGA TTTAACCTTT
    CAGCAAGGGG ATGTGATTGT GGTTACCAAG AAAGATGGTG ACTGGTGGAC GGGAACGGTG
    GGCGACAAGT CCGGAGTCTT CCCTTCTAAC TATGTGAGGC TTAAAGATTC AGAGGGCTCT
    GGAACTGCTG GGAAAACAGG GAGTTTAGGA AAAAAACCTG AAATTGCCCA GGTTATTGCT
    TCCTACGCTG CTACTGGTCC CGAACAACTC ACCCTGGCTC CTGGGCAGCT GATTCTGATC
    CGGAAAAAGA ACCCAGGTGG ATGGTGGGAA GGAGAACTGC AAGCTCGAGG GAAAAAGCGC
    CAGATAGGGT GGTTTCCAGC AAATTATGTC AAACTTCTAA GCCCCGGAAC AAGCAAAATC
    ACCCCAACTG AGCTACCCAA GACCGCAGTG CAGCCAGCAG TGTGCCAGGT GATCGGGATG
    TACGATTACA CCGCCCAGAA CGATGACGAA CTAGCCTTCA GCAAAGGCCA GATCATCAAC
    GTCCTCAACA AGGAGGACCC GGACTGGTGG AAAGGAGAAG TCAGTGGGCA AGTTGGGCTC
    TTCCCATCCA ATTATGTAAA GCTGACCACA GACATGGACC CCAGCCAGCA ATGGTGCTCA
    GACCTGCATC TCTTAGATAT GCTGACCCCG ACTGAGAGGA AGCGGCAAGG CTACATCCAT
    GAACTCATTG TCACGGAGGA GAACTACGTG AACGACTTGC AGCTGGTCAC AGAGATCTTT
    CAGAAACCCC TGACGGAGTC TGAGCTGCTG ACAGAAAAAG AGGTTGCTAT GATTTTTGTT
    AACTGGAAGG AGCTGATCAT GTGTAATATC AAACTGCTGA AAGCGCTGAG AGTCCGCAAG
    AAGATGTCTG GGGAGAAGAT GCCGGTGAAG ATGATTGGCG ACATCCTGAG CGCCCAGCTG
    CCGCACATGC AGCCTTACAT CCGCTTCTGC AGCTGCCAGC TCAATGGGGC TGCCCTCATC
    CAGCAGAAGA CGGACGAGGC TCCAGACTTC AAGGAGTTCG TCAAAAGACT GGCAATGGAC
    CCTCGGTGCA AAGGAATGCC TCTGTCCAGC TTTATACTGA AGCCTATGCA GCGTGTCACA
    AGATACCCGC TGATCATTAA AAACATCCTG GAAAACACTC CCGAGAACCA TCCAGACCAC
    AGCCACCTGA AGCATGCCCT GGAAAAGGCG GAGGAGCTAT GCTCCCAGGT GAACGAGGGA
    GTTCGAGAGA AGGAGAACTC AGACCGGCTG GAGTGGATCC AAGCCCACGT GCAGTGTGAA
    GGCCTTTCTG AGCAACTGGT GTTCAATTCA GTGACCAACT GCTTGGGACC ACGCAAGTTT
    CTGCACAGCG GGAAGCTCTA CAAGGCCAAG AGCAACAAAG AACTGTATGG CTTCCTCTTC
    AACGACTTCC TCCTGCTGAC CCAAATCACA AAGCCCTTAG GCTCTTCCGG CACCGACAAA
    GTCTTCAGCC CCAAATCTAA CCTTCAGTAT AAAATGTACA AAACGCCCAT TTTCTTAAAT
    GAGGTTCTAG TAAAATTGCC CACGGACCCT TCTGGAGATG AGCCTATCTT CCACATTTCC
    CACATCGACC GGGTCTACAC CCTCCGAGCA GAGAGCATAA ATGAGAGGAC TGCCTGGGTG
    CAGAAAATCA AGGCGGCGTC TGAGCTCTAC ATAGAGACGG AGAAAAAGAA GCGAGAGAAG
    GCGTACCTGG TCCGTTCCCA GCGGGCGACC GGTATTGGAA GGTTGATGGT GAACGTGGTA
    GAAGGCATTG AGCTGAAGCC CTGTCGGTCA CATGGAAAGA GCAACCCGTA CTGTGAGGTG
    ACCATGGGCT CTCAGTGCCA CATCACCAAG ACAATCCAGG ACACGCTAAA CCCCAAGTGG
    AATTCTAACT GCCAGTTCTT CATCAGAGAC CTGGAGCAGG AGGTTCTCTG CATCACAGTG
    TTTGAGAGGG ACCAGTTCTC GCCTGATGAT TTTTTGGGTC GGACAGAGAT CCGAGTGGCC
    GACATCAAGA AAGACCAGGG CTCCAAGGGG CCGGTTACGA AGTGTCTCCT GCTGCATGAG
    GTCCCCACGG GAGAGATTGT GGTCCGCCTT GACCTGCAGT TGTTTGATGA GCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522996.1
    CDS428..5644
    Translation

    Target ORF information:

    RefSeq Version XM_006522933.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522933.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTGGATGAAA GCCAGACTGG AGAGCCAGGA
    TGGCTTGGAG GAGAGCTGAA AGGGAAGACG GGATGGTTCC CTGCAAACTA TGCAGAAAAG
    ATTCCAGAAA ATGAGGTTCC CACTCCAGCC AAACCAGTGA CCGATCTGAC ATCTGCCCCT
    GCCCCCAAAC TGGCTCTGCG TGAGACCCCT GCTCCTTTGC CAGTGACCTC TTCTGAGCCC
    TCCACAACCC CCAACAACTG GGCAGACTTC AGTTCCACGT GGCCCAGCAG CTCAAACGAG
    AAGCCAGAAA CGGACAACTG GGATACATGG GCGGCTCAGC CTTCTCTGAC CGTACCTAGT
    GCTGGCCAGT TACGGCAGAG ATCAGCCTTT ACCCCAGCCA CAGCCACTGG CTCCTCCCCA
    TCTCCCGTCC TGGGCCAGGG TGAAAAGGTG GAAGGGCTAC AAGCGCAAGC CCTGTATCCC
    TGGAGAGCCA AAAAAGACAA CCACTTAAAT TTTAACAAAA GTGACGTCAT CACCGTTCTG
    GAACAGCAAG ACATGTGGTG GTTTGGAGAA GTTCAAGGTC AGAAGGGTTG GTTCCCCAAG
    TCTTACGTGA AACTCATTTC AGGGCCCGTA AGGAAATCCA CAAGCATCGA TACTGGCCCT
    ACTGAAAGTC CTGCTAGTCT AAAGAGAGTG GCTTCCCCGG CCGCCAAGCC AGCCATTCCC
    GGAGAAGAGT TTATTGCCAT GTACACATAC GAGAGTTCTG AGCAAGGAGA TTTAACCTTT
    CAGCAAGGGG ATGTGATTGT GGTTACCAAG AAAGATGGTG ACTGGTGGAC GGGAACGGTG
    GGCGACAAGT CCGGAGTCTT CCCTTCTAAC TATGTGAGGC TTAAAGATTC AGAGGGCTCT
    GGAACTGCTG GGAAAACAGG GAGTTTAGGA AAAAAACCTG AAATTGCCCA GGTTATTGCT
    TCCTACGCTG CTACTGGTCC CGAACAACTC ACCCTGGCTC CTGGGCAGCT GATTCTGATC
    CGGAAAAAGA ACCCAGGTGG ATGGTGGGAA GGAGAACTGC AAGCTCGAGG GAAAAAGCGC
    CAGATAGGGT GGTTTCCAGC AAATTATGTC AAACTTCTAA GCCCCGGAAC AAGCAAAATC
    ACCCCAACTG AGCTACCCAA GACCGCAGTG CAGCCAGCAG TGTGCCAGGT GATCGGGATG
    TACGATTACA CCGCCCAGAA CGATGACGAA CTAGCCTTCA GCAAAGGCCA GATCATCAAC
    GTCCTCAACA AGGAGGACCC GGACTGGTGG AAAGGAGAAG TCAGTGGGCA AGTTGGGCTC
    TTCCCATCCA ATTATGTAAA GCTGACCACA GACATGGACC CCAGCCAGCA ATGGTGCTCA
    GACCTGCATC TCTTAGATAT GCTGACCCCG ACTGAGAGGA AGCGGCAAGG CTACATCCAT
    GAACTCATTG TCACGGAGGA GAACTACGTG AACGACTTGC AGCTGGTCAC AGAGATCTTT
    CAGAAACCCC TGACGGAGTC TGAGCTGCTG ACAGAAAAAG AGGTTGCTAT GATTTTTGTT
    AACTGGAAGG AGCTGATCAT GTGTAATATC AAACTGCTGA AAGCGCTGAG AGTCCGCAAG
    AAGATGTCTG GGGAGAAGAT GCCGGTGAAG ATGATTGGCG ACATCCTGAG CGCCCAGCTG
    CCGCACATGC AGCCTTACAT CCGCTTCTGC AGCTGCCAGC TCAATGGGGC TGCCCTCATC
    CAGCAGAAGA CGGACGAGGC TCCAGACTTC AAGGAGTTCG TCAAAAGACT GGCAATGGAC
    CCTCGGTGCA AAGGAATGCC TCTGTCCAGC TTTATACTGA AGCCTATGCA GCGTGTCACA
    AGATACCCGC TGATCATTAA AAACATCCTG GAAAACACTC CCGAGAACCA TCCAGACCAC
    AGCCACCTGA AGCATGCCCT GGAAAAGGCG GAGGAGCTAT GCTCCCAGGT GAACGAGGGA
    GTTCGAGAGA AGGAGAACTC AGACCGGCTG GAGTGGATCC AAGCCCACGT GCAGTGTGAA
    GGCCTTTCTG AGCAACTGGT GTTCAATTCA GTGACCAACT GCTTGGGACC ACGCAAGTTT
    CTGCACAGCG GGAAGCTCTA CAAGGCCAAG AGCAACAAAG AACTGTATGG CTTCCTCTTC
    AACGACTTCC TCCTGCTGAC CCAAATCACA AAGCCCTTAG GCTCTTCCGG CACCGACAAA
    GTCTTCAGCC CCAAATCTAA CCTTCAGTAT AAAATGTACA AAACGCCCAT TTTCTTAAAT
    GAGGTTCTAG TAAAATTGCC CACGGACCCT TCTGGAGATG AGCCTATCTT CCACATTTCC
    CACATCGACC GGGTCTACAC CCTCCGAGCA GAGAGCATAA ATGAGAGGAC TGCCTGGGTG
    CAGAAAATCA AGGCGGCGTC TGAGCTCTAC ATAGAGACGG AGAAAAAGAA GCGAGAGAAG
    GCGTACCTGG TCCGTTCCCA GCGGGCGACC GGTATTGGAA GGTTGATGGT GAACGTGGTA
    GAAGGCATTG AGCTGAAGCC CTGTCGGTCA CATGGAAAGA GCAACCCGTA CTGTGAGGTG
    ACCATGGGCT CTCAGTGCCA CATCACCAAG ACAATCCAGG ACACGCTAAA CCCCAAGTGG
    AATTCTAACT GCCAGTTCTT CATCAGAGAC CTGGAGCAGG AGGTTCTCTG CATCACAGTG
    TTTGAGAGGG ACCAGTTCTC GCCTGATGAT TTTTTGGGTC GGACAGAGAT CCGAGTGGCC
    GACATCAAGA AAGACCAGGG CTCCAAGGGG CCGGTTACGA AGTGTCTCCT GCTGCATGAG
    GTCCCCACGG GAGAGATTGT GGTCCGCCTT GACCTGCAGT TGTTTGATGA GCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52686
    Clone ID Related Accession (Same CDS sequence) XM_006522934.4 , XM_006522934.3
    Accession Version XM_006522934.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5160bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522934.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCCACACC TTTCGGTGGT AGCCTGGATG TCTGGGCCAT AACTGTGGAG 
    GAAAGGGCCA AGCATGACCA GCAGTTCCTT AGCCTGAAGC CGATAGCGGG ATTTATTACT
    GGTGATCAAG CGAGGAACTT TTTTTTCCAA TCTGGGTTAC CTCAGCCTGT CTTAGCACAA
    ATATGGGCGC TAGCGGACAT GAATAACGAT GGAAGGATGG ATCAAGTGGA ATTTTCCATA
    GCCATGAAGC TTATCAAACT GAAGCTACAA GGATATCAGC TCCCCTCCAC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT GGCTATTTCC AGTGCACCAG CATTTGGTAT AGGAGGGATT
    GCTAGCATGC CACCACTCAC AGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCAGTTGTT
    GGAATGTCTC CACCCTTAGT ATCTTCTGTC CCTCCAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCTC CCGTCATACA GCCTCTGCCT GCGTTTGCGC ATCCTGCAGC CACATTGCCA
    AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAATCAT TCGATGTCGC CAGCGCCCCT CCAGCAGCAG AATGGGCTGT GCCTCAGTCA
    TCAAGGCTGA AATACAGGCA GTTATTCAAC AGCCACGACA AAACTATGAG TGGACACTTA
    ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACTGCAGA AGAATTTATC
    CTAGCTATGC ACCTAATTGA TGTTGCCATG TCTGGTCAGC CACTGCCGCC CGTCCTGCCT
    CCAGAATACA TCCCTCCTTC CTTCAGAAGA GTTCGCTCCG GCAGTGGGAT GTCCGTCATA
    AGCTCTTCTT CTGTGGATCA GAGGCTGCCT GAGGAGCCGT CGTCAGAGGA TGAGCAGCAG
    CCAGAGAAGA AACTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC
    AGTGTGGAGC TGGAGAAGCG CCGCCAAGCG CTCTTGGAGC AGCAGCGCAA AGAGCAGGAG
    CGGTTGGCTC AGCTGGAGCG CGCCGAGCAG GAGAGGAAAG AGCGGGAGCG CCAGGAGCAG
    GAGCGCAAGC GGCAGCTGGA GCTGGAGAAG CAGCTGGAGA AGCAGCGGGA GCTGGAGCGG
    CAGCGAGAGG AGGAGAGGAG GAAGGAGATC GAGAGGCGCG AGGCCGCAAA ACGGGAACTG
    GAAAGGCAGC GACAACTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GAATCAGAGG
    AACAAGGAGC AGGAGGGCAC CGTGGTCCTG AAGGCAAGGA GGAAGACTCT GGAGTTTGAG
    TTAGAAGCTC TGAATGACAA AAAGCATCAG CTAGAAGGAA AACTTCAGGA TATCAGGTGT
    CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACGAACA AGTCTAGAGA GCTAAGAATT
    GCTGAAATCA CCCACTTACA GCAGCAGTTG CAGGAATCTC AGCAAATGCT TGGAAGACTT
    ATTCCAGAGA AACAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGTTTGCAT
    AGAGACTCGC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAGCTGGC CCGGCAGCAG
    CTCCGGGAGC AGCTGGACGA GGTGGAGAGA GAGACCAGGT CAAAGCTGCA GGAGATTGAT
    GTTTTCAACA ACCAGCTGAA GGAACTGAGA GAGATACATA GCAAACAGCA ACTCCAGAAG
    CAGAGGTCCC TGGAGGCAGC GCGACTGAAG CAGAAAGAGC AGGAGAGGAA GAGCCTGGAG
    TTAGAGAAGC AAAAGGAAGA CGCTCAGAGA CGAGTTCAGG AAAGGGACAA GCAATGGCTG
    GAGCATGTGC AGCAGGAGGA GCAGCCACGC CCCCGGAAAC CCCACGAGGA GGACAGACTG
    AAGAGGGAAG ACAGTGTCAG GAAGAAGGAG GCGGAAGAGA GAGCCAAGCC GGAAATGCAA
    GACAAGCAGA GTCGGCTTTT CCATCCGCAT CAGGAGCCAG CTAAGCTGGC CACCCAGGCA
    CCCTGGTCTA CCACAGAGAA AGGCCCGCTT ACCATTTCTG CACAGGAGAG TGTAAAAGTG
    GTATATTACC GAGCGCTGTA CCCCTTTGAA TCCAGAAGTC ACGATGAGAT CACCATCCAG
    CCAGGAGATA TAGTCATGGT TAAAGGGGAA TGGGTGGATG AAAGCCAGAC TGGAGAGCCA
    GGATGGCTTG GAGGAGAGCT GAAAGGGAAG ACGGGATGGT TCCCTGCAAA CTATGCAGAA
    AAGATTCCAG AAAATGAGGT TCCCACTCCA GCCAAACCAG TGACCGATCT GACATCTGCC
    CCTGCCCCCA AACTGGCTCT GCGTGAGACC CCTGCTCCTT TGCCAGTGAC CTCTTCTGAG
    CCCTCCACAA CCCCCAACAA CTGGGCAGAC TTCAGTTCCA CGTGGCCCAG CAGCTCAAAC
    GAGAAGCCAG AAACGGACAA CTGGGATACA TGGGCGGCTC AGCCTTCTCT GACCGTACCT
    AGTGCTGGCC AGTTACGGCA GAGATCAGCC TTTACCCCAG CCACAGCCAC TGGCTCCTCC
    CCATCTCCCG TCCTGGGCCA GGGTGAAAAG GTGGAAGGGC TACAAGCGCA AGCCCTGTAT
    CCCTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAGTGACGT CATCACCGTT
    CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC
    AAGTCTTACG TGAAACTCAT TTCAGGGCCC GTAAGGAAAT CCACAAGCAT CGATACTGGC
    CCTACTGAAA GTCCTGCTAG TCTAAAGAGA GTGGCTTCCC CGGCCGCCAA GCCAGCCATT
    CCCGGAGAAG AGTTTATTGC CATGTACACA TACGAGAGTT CTGAGCAAGG AGATTTAACC
    TTTCAGCAAG GGGATGTGAT TGTGGTTACC AAGAAAGATG GTGACTGGTG GACGGGAACG
    GTGGGCGACA AGTCCGGAGT CTTCCCTTCT AACTATGTGA GGCTTAAAGA TTCAGAGGGC
    TCTGGAACTG CTGGGAAAAC AGGGAGTTTA GGAAAAAAAC CTGAAATTGC CCAGGTTATT
    GCTTCCTACG CTGCTACTGG TCCCGAACAA CTCACCCTGG CTCCTGGGCA GCTGATTCTG
    ATCCGGAAAA AGAACCCAGG TGGATGGTGG GAAGGAGAAC TGCAAGCTCG AGGGAAAAAG
    CGCCAGATAG GGTGGTTTCC AGCAAATTAT GTCAAACTTC TAAGCCCCGG AACAAGCAAA
    ATCACCCCAA CTGAGCTACC CAAGACCGCA GTGCAGCCAG CAGTGTGCCA GGTGATCGGG
    ATGTACGATT ACACCGCCCA GAACGATGAC GAACTAGCCT TCAGCAAAGG CCAGATCATC
    AACGTCCTCA ACAAGGAGGA CCCGGACTGG TGGAAAGGAG AAGTCAGTGG GCAAGTTGGG
    CTCTTCCCAT CCAATTATGT AAAGCTGACC ACAGACATGG ACCCCAGCCA GCAATGGTGC
    TCAGACCTGC ATCTCTTAGA TATGCTGACC CCGACTGAGA GGAAGCGGCA AGGCTACATC
    CATGAACTCA TTGTCACGGA GGAGAACTAC GTGAACGACT TGCAGCTGGT CACAGAGATC
    TTTCAGAAAC CCCTGACGGA GTCTGAGCTG CTGACAGAAA AAGAGGTTGC TATGATTTTT
    GTTAACTGGA AGGAGCTGAT CATGTGTAAT ATCAAACTGC TGAAAGCGCT GAGAGTCCGC
    AAGAAGATGT CTGGGGAGAA GATGCCGGTG AAGATGATTG GCGACATCCT GAGCGCCCAG
    CTGCCGCACA TGCAGCCTTA CATCCGCTTC TGCAGCTGCC AGCTCAATGG GGCTGCCCTC
    ATCCAGCAGA AGACGGACGA GGCTCCAGAC TTCAAGGAGT TCGTCAAAAG ACTGGCAATG
    GACCCTCGGT GCAAAGGAAT GCCTCTGTCC AGCTTTATAC TGAAGCCTAT GCAGCGTGTC
    ACAAGATACC CGCTGATCAT TAAAAACATC CTGGAAAACA CTCCCGAGAA CCATCCAGAC
    CACAGCCACC TGAAGCATGC CCTGGAAAAG GCGGAGGAGC TATGCTCCCA GGTGAACGAG
    GGAGTTCGAG AGAAGGAGAA CTCAGACCGG CTGGAGTGGA TCCAAGCCCA CGTGCAGTGT
    GAAGGCCTTT CTGAGCAACT GGTGTTCAAT TCAGTGACCA ACTGCTTGGG ACCACGCAAG
    TTTCTGCACA GCGGGAAGCT CTACAAGGCC AAGAGCAACA AAGAACTGTA TGGCTTCCTC
    TTCAACGACT TCCTCCTGCT GACCCAAATC ACAAAGCCCT TAGGCTCTTC CGGCACCGAC
    AAAGTCTTCA GCCCCAAATC TAACCTTCAG TATAAAATGT ACAAAACGCC CATTTTCTTA
    AATGAGGTTC TAGTAAAATT GCCCACGGAC CCTTCTGGAG ATGAGCCTAT CTTCCACATT
    TCCCACATCG ACCGGGTCTA CACCCTCCGA GCAGAGAGCA TAAATGAGAG GACTGCCTGG
    GTGCAGAAAA TCAAGGCGGC GTCTGAGCTC TACATAGAGA CGGAGAAAAA GAAGCGAGAG
    AAGGCGTACC TGGTCCGTTC CCAGCGGGCG ACCGGTATTG GAAGGTTGAT GGTGAACGTG
    GTAGAAGGCA TTGAGCTGAA GCCCTGTCGG TCACATGGAA AGAGCAACCC GTACTGTGAG
    GTGACCATGG GCTCTCAGTG CCACATCACC AAGACAATCC AGGACACGCT AAACCCCAAG
    TGGAATTCTA ACTGCCAGTT CTTCATCAGA GACCTGGAGC AGGAGGTTCT CTGCATCACA
    GTGTTTGAGA GGGACCAGTT CTCGCCTGAT GATTTTTTGG GTCGGACAGA GATCCGAGTG
    GCCGACATCA AGAAAGACCA GGGCTCCAAG GGGCCGGTTA CGAAGTGTCT CCTGCTGCAT
    GAGGTCCCCA CGGGAGAGAT TGTGGTCCGC CTTGACCTGC AGTTGTTTGA TGAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522997.1
    CDS280..5439
    Translation

    Target ORF information:

    RefSeq Version XM_006522934.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522934.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCCACACC TTTCGGTGGT AGCCTGGATG TCTGGGCCAT AACTGTGGAG 
    GAAAGGGCCA AGCATGACCA GCAGTTCCTT AGCCTGAAGC CGATAGCGGG ATTTATTACT
    GGTGATCAAG CGAGGAACTT TTTTTTCCAA TCTGGGTTAC CTCAGCCTGT CTTAGCACAA
    ATATGGGCGC TAGCGGACAT GAATAACGAT GGAAGGATGG ATCAAGTGGA ATTTTCCATA
    GCCATGAAGC TTATCAAACT GAAGCTACAA GGATATCAGC TCCCCTCCAC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT GGCTATTTCC AGTGCACCAG CATTTGGTAT AGGAGGGATT
    GCTAGCATGC CACCACTCAC AGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCAGTTGTT
    GGAATGTCTC CACCCTTAGT ATCTTCTGTC CCTCCAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCTC CCGTCATACA GCCTCTGCCT GCGTTTGCGC ATCCTGCAGC CACATTGCCA
    AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAATCAT TCGATGTCGC CAGCGCCCCT CCAGCAGCAG AATGGGCTGT GCCTCAGTCA
    TCAAGGCTGA AATACAGGCA GTTATTCAAC AGCCACGACA AAACTATGAG TGGACACTTA
    ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACTGCAGA AGAATTTATC
    CTAGCTATGC ACCTAATTGA TGTTGCCATG TCTGGTCAGC CACTGCCGCC CGTCCTGCCT
    CCAGAATACA TCCCTCCTTC CTTCAGAAGA GTTCGCTCCG GCAGTGGGAT GTCCGTCATA
    AGCTCTTCTT CTGTGGATCA GAGGCTGCCT GAGGAGCCGT CGTCAGAGGA TGAGCAGCAG
    CCAGAGAAGA AACTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC
    AGTGTGGAGC TGGAGAAGCG CCGCCAAGCG CTCTTGGAGC AGCAGCGCAA AGAGCAGGAG
    CGGTTGGCTC AGCTGGAGCG CGCCGAGCAG GAGAGGAAAG AGCGGGAGCG CCAGGAGCAG
    GAGCGCAAGC GGCAGCTGGA GCTGGAGAAG CAGCTGGAGA AGCAGCGGGA GCTGGAGCGG
    CAGCGAGAGG AGGAGAGGAG GAAGGAGATC GAGAGGCGCG AGGCCGCAAA ACGGGAACTG
    GAAAGGCAGC GACAACTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GAATCAGAGG
    AACAAGGAGC AGGAGGGCAC CGTGGTCCTG AAGGCAAGGA GGAAGACTCT GGAGTTTGAG
    TTAGAAGCTC TGAATGACAA AAAGCATCAG CTAGAAGGAA AACTTCAGGA TATCAGGTGT
    CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACGAACA AGTCTAGAGA GCTAAGAATT
    GCTGAAATCA CCCACTTACA GCAGCAGTTG CAGGAATCTC AGCAAATGCT TGGAAGACTT
    ATTCCAGAGA AACAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGTTTGCAT
    AGAGACTCGC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAGCTGGC CCGGCAGCAG
    CTCCGGGAGC AGCTGGACGA GGTGGAGAGA GAGACCAGGT CAAAGCTGCA GGAGATTGAT
    GTTTTCAACA ACCAGCTGAA GGAACTGAGA GAGATACATA GCAAACAGCA ACTCCAGAAG
    CAGAGGTCCC TGGAGGCAGC GCGACTGAAG CAGAAAGAGC AGGAGAGGAA GAGCCTGGAG
    TTAGAGAAGC AAAAGGAAGA CGCTCAGAGA CGAGTTCAGG AAAGGGACAA GCAATGGCTG
    GAGCATGTGC AGCAGGAGGA GCAGCCACGC CCCCGGAAAC CCCACGAGGA GGACAGACTG
    AAGAGGGAAG ACAGTGTCAG GAAGAAGGAG GCGGAAGAGA GAGCCAAGCC GGAAATGCAA
    GACAAGCAGA GTCGGCTTTT CCATCCGCAT CAGGAGCCAG CTAAGCTGGC CACCCAGGCA
    CCCTGGTCTA CCACAGAGAA AGGCCCGCTT ACCATTTCTG CACAGGAGAG TGTAAAAGTG
    GTATATTACC GAGCGCTGTA CCCCTTTGAA TCCAGAAGTC ACGATGAGAT CACCATCCAG
    CCAGGAGATA TAGTCATGGT TAAAGGGGAA TGGGTGGATG AAAGCCAGAC TGGAGAGCCA
    GGATGGCTTG GAGGAGAGCT GAAAGGGAAG ACGGGATGGT TCCCTGCAAA CTATGCAGAA
    AAGATTCCAG AAAATGAGGT TCCCACTCCA GCCAAACCAG TGACCGATCT GACATCTGCC
    CCTGCCCCCA AACTGGCTCT GCGTGAGACC CCTGCTCCTT TGCCAGTGAC CTCTTCTGAG
    CCCTCCACAA CCCCCAACAA CTGGGCAGAC TTCAGTTCCA CGTGGCCCAG CAGCTCAAAC
    GAGAAGCCAG AAACGGACAA CTGGGATACA TGGGCGGCTC AGCCTTCTCT GACCGTACCT
    AGTGCTGGCC AGTTACGGCA GAGATCAGCC TTTACCCCAG CCACAGCCAC TGGCTCCTCC
    CCATCTCCCG TCCTGGGCCA GGGTGAAAAG GTGGAAGGGC TACAAGCGCA AGCCCTGTAT
    CCCTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAGTGACGT CATCACCGTT
    CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC
    AAGTCTTACG TGAAACTCAT TTCAGGGCCC GTAAGGAAAT CCACAAGCAT CGATACTGGC
    CCTACTGAAA GTCCTGCTAG TCTAAAGAGA GTGGCTTCCC CGGCCGCCAA GCCAGCCATT
    CCCGGAGAAG AGTTTATTGC CATGTACACA TACGAGAGTT CTGAGCAAGG AGATTTAACC
    TTTCAGCAAG GGGATGTGAT TGTGGTTACC AAGAAAGATG GTGACTGGTG GACGGGAACG
    GTGGGCGACA AGTCCGGAGT CTTCCCTTCT AACTATGTGA GGCTTAAAGA TTCAGAGGGC
    TCTGGAACTG CTGGGAAAAC AGGGAGTTTA GGAAAAAAAC CTGAAATTGC CCAGGTTATT
    GCTTCCTACG CTGCTACTGG TCCCGAACAA CTCACCCTGG CTCCTGGGCA GCTGATTCTG
    ATCCGGAAAA AGAACCCAGG TGGATGGTGG GAAGGAGAAC TGCAAGCTCG AGGGAAAAAG
    CGCCAGATAG GGTGGTTTCC AGCAAATTAT GTCAAACTTC TAAGCCCCGG AACAAGCAAA
    ATCACCCCAA CTGAGCTACC CAAGACCGCA GTGCAGCCAG CAGTGTGCCA GGTGATCGGG
    ATGTACGATT ACACCGCCCA GAACGATGAC GAACTAGCCT TCAGCAAAGG CCAGATCATC
    AACGTCCTCA ACAAGGAGGA CCCGGACTGG TGGAAAGGAG AAGTCAGTGG GCAAGTTGGG
    CTCTTCCCAT CCAATTATGT AAAGCTGACC ACAGACATGG ACCCCAGCCA GCAATGGTGC
    TCAGACCTGC ATCTCTTAGA TATGCTGACC CCGACTGAGA GGAAGCGGCA AGGCTACATC
    CATGAACTCA TTGTCACGGA GGAGAACTAC GTGAACGACT TGCAGCTGGT CACAGAGATC
    TTTCAGAAAC CCCTGACGGA GTCTGAGCTG CTGACAGAAA AAGAGGTTGC TATGATTTTT
    GTTAACTGGA AGGAGCTGAT CATGTGTAAT ATCAAACTGC TGAAAGCGCT GAGAGTCCGC
    AAGAAGATGT CTGGGGAGAA GATGCCGGTG AAGATGATTG GCGACATCCT GAGCGCCCAG
    CTGCCGCACA TGCAGCCTTA CATCCGCTTC TGCAGCTGCC AGCTCAATGG GGCTGCCCTC
    ATCCAGCAGA AGACGGACGA GGCTCCAGAC TTCAAGGAGT TCGTCAAAAG ACTGGCAATG
    GACCCTCGGT GCAAAGGAAT GCCTCTGTCC AGCTTTATAC TGAAGCCTAT GCAGCGTGTC
    ACAAGATACC CGCTGATCAT TAAAAACATC CTGGAAAACA CTCCCGAGAA CCATCCAGAC
    CACAGCCACC TGAAGCATGC CCTGGAAAAG GCGGAGGAGC TATGCTCCCA GGTGAACGAG
    GGAGTTCGAG AGAAGGAGAA CTCAGACCGG CTGGAGTGGA TCCAAGCCCA CGTGCAGTGT
    GAAGGCCTTT CTGAGCAACT GGTGTTCAAT TCAGTGACCA ACTGCTTGGG ACCACGCAAG
    TTTCTGCACA GCGGGAAGCT CTACAAGGCC AAGAGCAACA AAGAACTGTA TGGCTTCCTC
    TTCAACGACT TCCTCCTGCT GACCCAAATC ACAAAGCCCT TAGGCTCTTC CGGCACCGAC
    AAAGTCTTCA GCCCCAAATC TAACCTTCAG TATAAAATGT ACAAAACGCC CATTTTCTTA
    AATGAGGTTC TAGTAAAATT GCCCACGGAC CCTTCTGGAG ATGAGCCTAT CTTCCACATT
    TCCCACATCG ACCGGGTCTA CACCCTCCGA GCAGAGAGCA TAAATGAGAG GACTGCCTGG
    GTGCAGAAAA TCAAGGCGGC GTCTGAGCTC TACATAGAGA CGGAGAAAAA GAAGCGAGAG
    AAGGCGTACC TGGTCCGTTC CCAGCGGGCG ACCGGTATTG GAAGGTTGAT GGTGAACGTG
    GTAGAAGGCA TTGAGCTGAA GCCCTGTCGG TCACATGGAA AGAGCAACCC GTACTGTGAG
    GTGACCATGG GCTCTCAGTG CCACATCACC AAGACAATCC AGGACACGCT AAACCCCAAG
    TGGAATTCTA ACTGCCAGTT CTTCATCAGA GACCTGGAGC AGGAGGTTCT CTGCATCACA
    GTGTTTGAGA GGGACCAGTT CTCGCCTGAT GATTTTTTGG GTCGGACAGA GATCCGAGTG
    GCCGACATCA AGAAAGACCA GGGCTCCAAG GGGCCGGTTA CGAAGTGTCT CCTGCTGCAT
    GAGGTCCCCA CGGGAGAGAT TGTGGTCCGC CTTGACCTGC AGTTGTTTGA TGAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52687
    Clone ID Related Accession (Same CDS sequence) XM_006522935.2
    Accession Version XM_006522935.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522935.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGGGTGAAA AGGTGGAAGG GCTACAAGCG
    CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA GACAACCACT TAAATTTTAA CAAAAGTGAC
    GTCATCACCG TTCTGGAACA GCAAGACATG TGGTGGTTTG GAGAAGTTCA AGGTCAGAAG
    GGTTGGTTCC CCAAGTCTTA CGTGAAACTC ATTTCAGGGC CCGTAAGGAA ATCCACAAGC
    ATCGATACTG GCCCTACTGA AAGTCCTGCT AGTCTAAAGA GAGTGGCTTC CCCGGCCGCC
    AAGCCAGCCA TTCCCGGAGA AGAAATTGCC CAGGTTATTG CTTCCTACGC TGCTACTGGT
    CCCGAACAAC TCACCCTGGC TCCTGGGCAG CTGATTCTGA TCCGGAAAAA GAACCCAGGT
    GGATGGTGGG AAGGAGAACT GCAAGCTCGA GGGAAAAAGC GCCAGATAGG GTGGTTTCCA
    GCAAATTATG TCAAACTTCT AAGCCCCGGA ACAAGCAAAA TCACCCCAAC TGAGCTACCC
    AAGACCGCAG TGCAGCCAGC AGTGTGCCAG GTGATCGGGA TGTACGATTA CACCGCCCAG
    AACGATGACG AACTAGCCTT CAGCAAAGGC CAGATCATCA ACGTCCTCAA CAAGGAGGAC
    CCGGACTGGT GGAAAGGAGA AGTCAGTGGG CAAGTTGGGC TCTTCCCATC CAATTATGTA
    AAGCTGACCA CAGACATGGA CCCCAGCCAG CAATGGTGCT CAGACCTGCA TCTCTTAGAT
    ATGCTGACCC CGACTGAGAG GAAGCGGCAA GGCTACATCC ATGAACTCAT TGTCACGGAG
    GAGAACTACG TGAACGACTT GCAGCTGGTC ACAGAGATCT TTCAGAAACC CCTGACGGAG
    TCTGAGCTGC TGACAGAAAA AGAGGTTGCT ATGATTTTTG TTAACTGGAA GGAGCTGATC
    ATGTGTAATA TCAAACTGCT GAAAGCGCTG AGAGTCCGCA AGAAGATGTC TGGGGAGAAG
    ATGCCGGTGA AGATGATTGG CGACATCCTG AGCGCCCAGC TGCCGCACAT GCAGCCTTAC
    ATCCGCTTCT GCAGCTGCCA GCTCAATGGG GCTGCCCTCA TCCAGCAGAA GACGGACGAG
    GCTCCAGACT TCAAGGAGTT CGTCAAAAGA CTGGCAATGG ACCCTCGGTG CAAAGGAATG
    CCTCTGTCCA GCTTTATACT GAAGCCTATG CAGCGTGTCA CAAGATACCC GCTGATCATT
    AAAAACATCC TGGAAAACAC TCCCGAGAAC CATCCAGACC ACAGCCACCT GAAGCATGCC
    CTGGAAAAGG CGGAGGAGCT ATGCTCCCAG GTGAACGAGG GAGTTCGAGA GAAGGAGAAC
    TCAGACCGGC TGGAGTGGAT CCAAGCCCAC GTGCAGTGTG AAGGCCTTTC TGAGCAACTG
    GTGTTCAATT CAGTGACCAA CTGCTTGGGA CCACGCAAGT TTCTGCACAG CGGGAAGCTC
    TACAAGGCCA AGAGCAACAA AGAACTGTAT GGCTTCCTCT TCAACGACTT CCTCCTGCTG
    ACCCAAATCA CAAAGCCCTT AGGCTCTTCC GGCACCGACA AAGTCTTCAG CCCCAAATCT
    AACCTTCAGT ATAAAATGTA CAAAACGCCC ATTTTCTTAA ATGAGGTTCT AGTAAAATTG
    CCCACGGACC CTTCTGGAGA TGAGCCTATC TTCCACATTT CCCACATCGA CCGGGTCTAC
    ACCCTCCGAG CAGAGAGCAT AAATGAGAGG ACTGCCTGGG TGCAGAAAAT CAAGGCGGCG
    TCTGAGCTCT ACATAGAGAC GGAGAAAAAG AAGCGAGAGA AGGCGTACCT GGTCCGTTCC
    CAGCGGGCGA CCGGTATTGG AAGGTTGATG GTGAACGTGG TAGAAGGCAT TGAGCTGAAG
    CCCTGTCGGT CACATGGAAA GAGCAACCCG TACTGTGAGG TGACCATGGG CTCTCAGTGC
    CACATCACCA AGACAATCCA GGACACGCTA AACCCCAAGT GGAATTCTAA CTGCCAGTTC
    TTCATCAGAG ACCTGGAGCA GGAGGTTCTC TGCATCACAG TGTTTGAGAG GGACCAGTTC
    TCGCCTGATG ATTTTTTGGG TCGGACAGAG ATCCGAGTGG CCGACATCAA GAAAGACCAG
    GGCTCCAAGG GGCCGGTTAC GAAGTGTCTC CTGCTGCATG AGGTCCCCAC GGGAGAGATT
    GTGGTCCGCC TTGACCTGCA GTTGTTTGAT GAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522998.1
    CDS428..5446
    Translation

    Target ORF information:

    RefSeq Version XM_006522935.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522935.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGGGTGAAA AGGTGGAAGG GCTACAAGCG
    CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA GACAACCACT TAAATTTTAA CAAAAGTGAC
    GTCATCACCG TTCTGGAACA GCAAGACATG TGGTGGTTTG GAGAAGTTCA AGGTCAGAAG
    GGTTGGTTCC CCAAGTCTTA CGTGAAACTC ATTTCAGGGC CCGTAAGGAA ATCCACAAGC
    ATCGATACTG GCCCTACTGA AAGTCCTGCT AGTCTAAAGA GAGTGGCTTC CCCGGCCGCC
    AAGCCAGCCA TTCCCGGAGA AGAAATTGCC CAGGTTATTG CTTCCTACGC TGCTACTGGT
    CCCGAACAAC TCACCCTGGC TCCTGGGCAG CTGATTCTGA TCCGGAAAAA GAACCCAGGT
    GGATGGTGGG AAGGAGAACT GCAAGCTCGA GGGAAAAAGC GCCAGATAGG GTGGTTTCCA
    GCAAATTATG TCAAACTTCT AAGCCCCGGA ACAAGCAAAA TCACCCCAAC TGAGCTACCC
    AAGACCGCAG TGCAGCCAGC AGTGTGCCAG GTGATCGGGA TGTACGATTA CACCGCCCAG
    AACGATGACG AACTAGCCTT CAGCAAAGGC CAGATCATCA ACGTCCTCAA CAAGGAGGAC
    CCGGACTGGT GGAAAGGAGA AGTCAGTGGG CAAGTTGGGC TCTTCCCATC CAATTATGTA
    AAGCTGACCA CAGACATGGA CCCCAGCCAG CAATGGTGCT CAGACCTGCA TCTCTTAGAT
    ATGCTGACCC CGACTGAGAG GAAGCGGCAA GGCTACATCC ATGAACTCAT TGTCACGGAG
    GAGAACTACG TGAACGACTT GCAGCTGGTC ACAGAGATCT TTCAGAAACC CCTGACGGAG
    TCTGAGCTGC TGACAGAAAA AGAGGTTGCT ATGATTTTTG TTAACTGGAA GGAGCTGATC
    ATGTGTAATA TCAAACTGCT GAAAGCGCTG AGAGTCCGCA AGAAGATGTC TGGGGAGAAG
    ATGCCGGTGA AGATGATTGG CGACATCCTG AGCGCCCAGC TGCCGCACAT GCAGCCTTAC
    ATCCGCTTCT GCAGCTGCCA GCTCAATGGG GCTGCCCTCA TCCAGCAGAA GACGGACGAG
    GCTCCAGACT TCAAGGAGTT CGTCAAAAGA CTGGCAATGG ACCCTCGGTG CAAAGGAATG
    CCTCTGTCCA GCTTTATACT GAAGCCTATG CAGCGTGTCA CAAGATACCC GCTGATCATT
    AAAAACATCC TGGAAAACAC TCCCGAGAAC CATCCAGACC ACAGCCACCT GAAGCATGCC
    CTGGAAAAGG CGGAGGAGCT ATGCTCCCAG GTGAACGAGG GAGTTCGAGA GAAGGAGAAC
    TCAGACCGGC TGGAGTGGAT CCAAGCCCAC GTGCAGTGTG AAGGCCTTTC TGAGCAACTG
    GTGTTCAATT CAGTGACCAA CTGCTTGGGA CCACGCAAGT TTCTGCACAG CGGGAAGCTC
    TACAAGGCCA AGAGCAACAA AGAACTGTAT GGCTTCCTCT TCAACGACTT CCTCCTGCTG
    ACCCAAATCA CAAAGCCCTT AGGCTCTTCC GGCACCGACA AAGTCTTCAG CCCCAAATCT
    AACCTTCAGT ATAAAATGTA CAAAACGCCC ATTTTCTTAA ATGAGGTTCT AGTAAAATTG
    CCCACGGACC CTTCTGGAGA TGAGCCTATC TTCCACATTT CCCACATCGA CCGGGTCTAC
    ACCCTCCGAG CAGAGAGCAT AAATGAGAGG ACTGCCTGGG TGCAGAAAAT CAAGGCGGCG
    TCTGAGCTCT ACATAGAGAC GGAGAAAAAG AAGCGAGAGA AGGCGTACCT GGTCCGTTCC
    CAGCGGGCGA CCGGTATTGG AAGGTTGATG GTGAACGTGG TAGAAGGCAT TGAGCTGAAG
    CCCTGTCGGT CACATGGAAA GAGCAACCCG TACTGTGAGG TGACCATGGG CTCTCAGTGC
    CACATCACCA AGACAATCCA GGACACGCTA AACCCCAAGT GGAATTCTAA CTGCCAGTTC
    TTCATCAGAG ACCTGGAGCA GGAGGTTCTC TGCATCACAG TGTTTGAGAG GGACCAGTTC
    TCGCCTGATG ATTTTTTGGG TCGGACAGAG ATCCGAGTGG CCGACATCAA GAAAGACCAG
    GGCTCCAAGG GGCCGGTTAC GAAGTGTCTC CTGCTGCATG AGGTCCCCAC GGGAGAGATT
    GTGGTCCGCC TTGACCTGCA GTTGTTTGAT GAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52688
    Clone ID Related Accession (Same CDS sequence) XM_006522936.2
    Accession Version XM_006522936.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTGGATGAAA GCCAGACTGG AGAGCCAGGA
    TGGCTTGGAG GAGAGCTGAA AGGGAAGACG GGATGGTTCC CTGCAAACTA TGCAGAAAAG
    ATTCCAGAAA ATGAGGTTCC CACTCCAGCC AAACCAGTGA CCGATCTGAC ATCTGCCCCT
    GCCCCCAAAC TGGCTCTGCG TGAGACCCCT GCTCCTTTGC CAGTGACCTC TTCTGAGCCC
    TCCACAACCC CCAACAACTG GGCAGACTTC AGTTCCACGT GGCCCAGCAG CTCAAACGAG
    AAGCCAGAAA CGGACAACTG GGATACATGG GCGGCTCAGC CTTCTCTGAC CGTACCTAGT
    GCTGGCCAGT TACGGCAGAG ATCAGCCTTT ACCCCAGCCA CAGCCACTGG CTCCTCCCCA
    TCTCCCGTCC TGGGCCAGGG TGAAAAGGTG GAAGGGCTAC AAGCGCAAGC CCTGTATCCC
    TGGAGAGCCA AAAAAGACAA CCACTTAAAT TTTAACAAAA GTGACGTCAT CACCGTTCTG
    GAACAGCAAG ACATGTGGTG GTTTGGAGAA GTTCAAGGTC AGAAGGGTTG GTTCCCCAAG
    TCTTACGTGA AACTCATTTC AGGGCCCGTA AGGAAATCCA CAAGCATCGA TACTGGCCCT
    ACTGAAAGTC CTGCTAGTCT AAAGAGAGTG GCTTCCCCGG CCGCCAAGCC AGCCATTCCC
    GGAGAAGAAA TTGCCCAGGT TATTGCTTCC TACGCTGCTA CTGGTCCCGA ACAACTCACC
    CTGGCTCCTG GGCAGCTGAT TCTGATCCGG AAAAAGAACC CAGGTGGATG GTGGGAAGGA
    GAACTGCAAG CTCGAGGGAA AAAGCGCCAG ATAGGGTGGT TTCCAGCAAA TTATGTCAAA
    CTTCTAAGCC CCGGAACAAG CAAAATCACC CCAACTGAGC TACCCAAGAC CGCAGTGCAG
    CCAGCAGTGT GCCAGGTGAT CGGGATGTAC GATTACACCG CCCAGAACGA TGACGAACTA
    GCCTTCAGCA AAGGCCAGAT CATCAACGTC CTCAACAAGG AGGACCCGGA CTGGTGGAAA
    GGAGAAGTCA GTGGGCAAGT TGGGCTCTTC CCATCCAATT ATGTAAAGCT GACCACAGAC
    ATGGACCCCA GCCAGCAATG GTGCTCAGAC CTGCATCTCT TAGATATGCT GACCCCGACT
    GAGAGGAAGC GGCAAGGCTA CATCCATGAA CTCATTGTCA CGGAGGAGAA CTACGTGAAC
    GACTTGCAGC TGGTCACAGA GATCTTTCAG AAACCCCTGA CGGAGTCTGA GCTGCTGACA
    GAAAAAGAGG TTGCTATGAT TTTTGTTAAC TGGAAGGAGC TGATCATGTG TAATATCAAA
    CTGCTGAAAG CGCTGAGAGT CCGCAAGAAG ATGTCTGGGG AGAAGATGCC GGTGAAGATG
    ATTGGCGACA TCCTGAGCGC CCAGCTGCCG CACATGCAGC CTTACATCCG CTTCTGCAGC
    TGCCAGCTCA ATGGGGCTGC CCTCATCCAG CAGAAGACGG ACGAGGCTCC AGACTTCAAG
    GAGTTCGTCA AAAGACTGGC AATGGACCCT CGGTGCAAAG GAATGCCTCT GTCCAGCTTT
    ATACTGAAGC CTATGCAGCG TGTCACAAGA TACCCGCTGA TCATTAAAAA CATCCTGGAA
    AACACTCCCG AGAACCATCC AGACCACAGC CACCTGAAGC ATGCCCTGGA AAAGGCGGAG
    GAGCTATGCT CCCAGGTGAA CGAGGGAGTT CGAGAGAAGG AGAACTCAGA CCGGCTGGAG
    TGGATCCAAG CCCACGTGCA GTGTGAAGGC CTTTCTGAGC AACTGGTGTT CAATTCAGTG
    ACCAACTGCT TGGGACCACG CAAGTTTCTG CACAGCGGGA AGCTCTACAA GGCCAAGAGC
    AACAAAGAAC TGTATGGCTT CCTCTTCAAC GACTTCCTCC TGCTGACCCA AATCACAAAG
    CCCTTAGGCT CTTCCGGCAC CGACAAAGTC TTCAGCCCCA AATCTAACCT TCAGTATAAA
    ATGTACAAAA CGCCCATTTT CTTAAATGAG GTTCTAGTAA AATTGCCCAC GGACCCTTCT
    GGAGATGAGC CTATCTTCCA CATTTCCCAC ATCGACCGGG TCTACACCCT CCGAGCAGAG
    AGCATAAATG AGAGGACTGC CTGGGTGCAG AAAATCAAGG CGGCGTCTGA GCTCTACATA
    GAGACGGAGA AAAAGAAGCG AGAGAAGGCG TACCTGGTCC GTTCCCAGCG GGCGACCGGT
    ATTGGAAGGT TGATGGTGAA CGTGGTAGAA GGCATTGAGC TGAAGCCCTG TCGGTCACAT
    GGAAAGAGCA ACCCGTACTG TGAGGTGACC ATGGGCTCTC AGTGCCACAT CACCAAGACA
    ATCCAGGACA CGCTAAACCC CAAGTGGAAT TCTAACTGCC AGTTCTTCAT CAGAGACCTG
    GAGCAGGAGG TTCTCTGCAT CACAGTGTTT GAGAGGGACC AGTTCTCGCC TGATGATTTT
    TTGGGTCGGA CAGAGATCCG AGTGGCCGAC ATCAAGAAAG ACCAGGGCTC CAAGGGGCCG
    GTTACGAAGT GTCTCCTGCT GCATGAGGTC CCCACGGGAG AGATTGTGGT CCGCCTTGAC
    CTGCAGTTGT TTGATGAGCC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522999.1
    CDS428..5431
    Translation

    Target ORF information:

    RefSeq Version XM_006522936.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522936.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTGGATGAAA GCCAGACTGG AGAGCCAGGA
    TGGCTTGGAG GAGAGCTGAA AGGGAAGACG GGATGGTTCC CTGCAAACTA TGCAGAAAAG
    ATTCCAGAAA ATGAGGTTCC CACTCCAGCC AAACCAGTGA CCGATCTGAC ATCTGCCCCT
    GCCCCCAAAC TGGCTCTGCG TGAGACCCCT GCTCCTTTGC CAGTGACCTC TTCTGAGCCC
    TCCACAACCC CCAACAACTG GGCAGACTTC AGTTCCACGT GGCCCAGCAG CTCAAACGAG
    AAGCCAGAAA CGGACAACTG GGATACATGG GCGGCTCAGC CTTCTCTGAC CGTACCTAGT
    GCTGGCCAGT TACGGCAGAG ATCAGCCTTT ACCCCAGCCA CAGCCACTGG CTCCTCCCCA
    TCTCCCGTCC TGGGCCAGGG TGAAAAGGTG GAAGGGCTAC AAGCGCAAGC CCTGTATCCC
    TGGAGAGCCA AAAAAGACAA CCACTTAAAT TTTAACAAAA GTGACGTCAT CACCGTTCTG
    GAACAGCAAG ACATGTGGTG GTTTGGAGAA GTTCAAGGTC AGAAGGGTTG GTTCCCCAAG
    TCTTACGTGA AACTCATTTC AGGGCCCGTA AGGAAATCCA CAAGCATCGA TACTGGCCCT
    ACTGAAAGTC CTGCTAGTCT AAAGAGAGTG GCTTCCCCGG CCGCCAAGCC AGCCATTCCC
    GGAGAAGAAA TTGCCCAGGT TATTGCTTCC TACGCTGCTA CTGGTCCCGA ACAACTCACC
    CTGGCTCCTG GGCAGCTGAT TCTGATCCGG AAAAAGAACC CAGGTGGATG GTGGGAAGGA
    GAACTGCAAG CTCGAGGGAA AAAGCGCCAG ATAGGGTGGT TTCCAGCAAA TTATGTCAAA
    CTTCTAAGCC CCGGAACAAG CAAAATCACC CCAACTGAGC TACCCAAGAC CGCAGTGCAG
    CCAGCAGTGT GCCAGGTGAT CGGGATGTAC GATTACACCG CCCAGAACGA TGACGAACTA
    GCCTTCAGCA AAGGCCAGAT CATCAACGTC CTCAACAAGG AGGACCCGGA CTGGTGGAAA
    GGAGAAGTCA GTGGGCAAGT TGGGCTCTTC CCATCCAATT ATGTAAAGCT GACCACAGAC
    ATGGACCCCA GCCAGCAATG GTGCTCAGAC CTGCATCTCT TAGATATGCT GACCCCGACT
    GAGAGGAAGC GGCAAGGCTA CATCCATGAA CTCATTGTCA CGGAGGAGAA CTACGTGAAC
    GACTTGCAGC TGGTCACAGA GATCTTTCAG AAACCCCTGA CGGAGTCTGA GCTGCTGACA
    GAAAAAGAGG TTGCTATGAT TTTTGTTAAC TGGAAGGAGC TGATCATGTG TAATATCAAA
    CTGCTGAAAG CGCTGAGAGT CCGCAAGAAG ATGTCTGGGG AGAAGATGCC GGTGAAGATG
    ATTGGCGACA TCCTGAGCGC CCAGCTGCCG CACATGCAGC CTTACATCCG CTTCTGCAGC
    TGCCAGCTCA ATGGGGCTGC CCTCATCCAG CAGAAGACGG ACGAGGCTCC AGACTTCAAG
    GAGTTCGTCA AAAGACTGGC AATGGACCCT CGGTGCAAAG GAATGCCTCT GTCCAGCTTT
    ATACTGAAGC CTATGCAGCG TGTCACAAGA TACCCGCTGA TCATTAAAAA CATCCTGGAA
    AACACTCCCG AGAACCATCC AGACCACAGC CACCTGAAGC ATGCCCTGGA AAAGGCGGAG
    GAGCTATGCT CCCAGGTGAA CGAGGGAGTT CGAGAGAAGG AGAACTCAGA CCGGCTGGAG
    TGGATCCAAG CCCACGTGCA GTGTGAAGGC CTTTCTGAGC AACTGGTGTT CAATTCAGTG
    ACCAACTGCT TGGGACCACG CAAGTTTCTG CACAGCGGGA AGCTCTACAA GGCCAAGAGC
    AACAAAGAAC TGTATGGCTT CCTCTTCAAC GACTTCCTCC TGCTGACCCA AATCACAAAG
    CCCTTAGGCT CTTCCGGCAC CGACAAAGTC TTCAGCCCCA AATCTAACCT TCAGTATAAA
    ATGTACAAAA CGCCCATTTT CTTAAATGAG GTTCTAGTAA AATTGCCCAC GGACCCTTCT
    GGAGATGAGC CTATCTTCCA CATTTCCCAC ATCGACCGGG TCTACACCCT CCGAGCAGAG
    AGCATAAATG AGAGGACTGC CTGGGTGCAG AAAATCAAGG CGGCGTCTGA GCTCTACATA
    GAGACGGAGA AAAAGAAGCG AGAGAAGGCG TACCTGGTCC GTTCCCAGCG GGCGACCGGT
    ATTGGAAGGT TGATGGTGAA CGTGGTAGAA GGCATTGAGC TGAAGCCCTG TCGGTCACAT
    GGAAAGAGCA ACCCGTACTG TGAGGTGACC ATGGGCTCTC AGTGCCACAT CACCAAGACA
    ATCCAGGACA CGCTAAACCC CAAGTGGAAT TCTAACTGCC AGTTCTTCAT CAGAGACCTG
    GAGCAGGAGG TTCTCTGCAT CACAGTGTTT GAGAGGGACC AGTTCTCGCC TGATGATTTT
    TTGGGTCGGA CAGAGATCCG AGTGGCCGAC ATCAAGAAAG ACCAGGGCTC CAAGGGGCCG
    GTTACGAAGT GTCTCCTGCT GCATGAGGTC CCCACGGGAG AGATTGTGGT CCGCCTTGAC
    CTGCAGTTGT TTGATGAGCC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52689
    Clone ID Related Accession (Same CDS sequence) XM_006522937.3
    Accession Version XM_006522937.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4581bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522937.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGAGAAG TGTTGCAGAA GTACTTACAG CAGCGTCCAG CGTGCGCCCC TCCAGCAGCA 
    GAATGGGCTG TGCCTCAGTC ATCAAGGCTG AAATACAGGC AGTTATTCAA CAGCCACGAC
    AAAACTATGA GTGGACACTT AACAGGTCCC CAGGCAAGAA CTATTCTCAT GCAATCAAGT
    TTACCCCAGG CTCAGCTGGC TTCAATATGG AATCTTTCTG ACATTGATCA AGATGGAAAA
    CTCACTGCAG AAGAATTTAT CCTAGCTATG CACCTAATTG ATGTTGCCAT GTCTGGTCAG
    CCACTGCCGC CCGTCCTGCC TCCAGAATAC ATCCCTCCTT CCTTCAGAAG AGTTCGCTCC
    GGCAGTGGGA TGTCCGTCAT AAGCTCTTCT TCTGTGGATC AGAGGCTGCC TGAGGAGCCG
    TCGTCAGAGG ATGAGCAGCA GCCAGAGAAG AAACTGCCTG TGACATTTGA AGATAAGAAG
    CGGGAGAACT TCGAGCGAGG CAGTGTGGAG CTGGAGAAGC GCCGCCAAGC GCTCTTGGAG
    CAGCAGCGCA AAGAGCAGGA GCGGTTGGCT CAGCTGGAGC GCGCCGAGCA GGAGAGGAAA
    GAGCGGGAGC GCCAGGAGCA GGAGCGCAAG CGGCAGCTGG AGCTGGAGAA GCAGCTGGAG
    AAGCAGCGGG AGCTGGAGCG GCAGCGAGAG GAGGAGAGGA GGAAGGAGAT CGAGAGGCGC
    GAGGCCGCAA AACGGGAACT GGAAAGGCAG CGACAACTTG AATGGGAACG GAACCGGAGA
    CAGGAACTCC TGAATCAGAG GAACAAGGAG CAGGAGGGCA CCGTGGTCCT GAAGGCAAGG
    AGGAAGACTC TGGAGTTTGA GTTAGAAGCT CTGAATGACA AAAAGCATCA GCTAGAAGGA
    AAACTTCAGG ATATCAGGTG TCGACTGGCA ACCCAGAGGC AAGAAATTGA GAGCACGAAC
    AAGTCTAGAG AGCTAAGAAT TGCTGAAATC ACCCACTTAC AGCAGCAGTT GCAGGAATCT
    CAGCAAATGC TTGGAAGACT TATTCCAGAG AAACAGATAC TCAGTGACCA GTTAAAACAA
    GTCCAGCAGA ACAGTTTGCA TAGAGACTCG CTTCTTACCC TCAAAAGAGC CTTGGAAGCA
    AAGGAGCTGG CCCGGCAGCA GCTCCGGGAG CAGCTGGACG AGGTGGAGAG AGAGACCAGG
    TCAAAGCTGC AGGAGATTGA TGTTTTCAAC AACCAGCTGA AGGAACTGAG AGAGATACAT
    AGCAAACAGC AACTCCAGAA GCAGAGGTCC CTGGAGGCAG CGCGACTGAA GCAGAAAGAG
    CAGGAGAGGA AGAGCCTGGA GTTAGAGAAG CAAAAGGAAG ACGCTCAGAG ACGAGTTCAG
    GAAAGGGACA AGCAATGGCT GGAGCATGTG CAGCAGGAGG AGCAGCCACG CCCCCGGAAA
    CCCCACGAGG AGGACAGACT GAAGAGGGAA GACAGTGTCA GGAAGAAGGA GGCGGAAGAG
    AGAGCCAAGC CGGAAATGCA AGACAAGCAG AGTCGGCTTT TCCATCCGCA TCAGGAGCCA
    GCTAAGCTGG CCACCCAGGC ACCCTGGTCT ACCACAGAGA AAGGCCCGCT TACCATTTCT
    GCACAGGAGA GTGTAAAAGT GGTATATTAC CGAGCGCTGT ACCCCTTTGA ATCCAGAAGT
    CACGATGAGA TCACCATCCA GCCAGGAGAT ATAGTCATGG TTAAAGGGGA ATGGGTGGAT
    GAAAGCCAGA CTGGAGAGCC AGGATGGCTT GGAGGAGAGC TGAAAGGGAA GACGGGATGG
    TTCCCTGCAA ACTATGCAGA AAAGATTCCA GAAAATGAGG TTCCCACTCC AGCCAAACCA
    GTGACCGATC TGACATCTGC CCCTGCCCCC AAACTGGCTC TGCGTGAGAC CCCTGCTCCT
    TTGCCAGTGA CCTCTTCTGA GCCCTCCACA ACCCCCAACA ACTGGGCAGA CTTCAGTTCC
    ACGTGGCCCA GCAGCTCAAA CGAGAAGCCA GAAACGGACA ACTGGGATAC ATGGGCGGCT
    CAGCCTTCTC TGACCGTACC TAGTGCTGGC CAGTTACGGC AGAGATCAGC CTTTACCCCA
    GCCACAGCCA CTGGCTCCTC CCCATCTCCC GTCCTGGGCC AGGGTGAAAA GGTGGAAGGG
    CTACAAGCGC AAGCCCTGTA TCCCTGGAGA GCCAAAAAAG ACAACCACTT AAATTTTAAC
    AAAAGTGACG TCATCACCGT TCTGGAACAG CAAGACATGT GGTGGTTTGG AGAAGTTCAA
    GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC GTGAAACTCA TTTCAGGGCC CGTAAGGAAA
    TCCACAAGCA TCGATACTGG CCCTACTGAA AGTCCTGCTA GTCTAAAGAG AGTGGCTTCC
    CCGGCCGCCA AGCCAGCCAT TCCCGGAGAA GAGTTTATTG CCATGTACAC ATACGAGAGT
    TCTGAGCAAG GAGATTTAAC CTTTCAGCAA GGGGATGTGA TTGTGGTTAC CAAGAAAGAT
    GGTGACTGGT GGACGGGAAC GGTGGGCGAC AAGTCCGGAG TCTTCCCTTC TAACTATGTG
    AGGCTTAAAG ATTCAGAGGG CTCTGGAACT GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA
    CCTGAAATTG CCCAGGTTAT TGCTTCCTAC GCTGCTACTG GTCCCGAACA ACTCACCCTG
    GCTCCTGGGC AGCTGATTCT GATCCGGAAA AAGAACCCAG GTGGATGGTG GGAAGGAGAA
    CTGCAAGCTC GAGGGAAAAA GCGCCAGATA GGGTGGTTTC CAGCAAATTA TGTCAAACTT
    CTAAGCCCCG GAACAAGCAA AATCACCCCA ACTGAGCTAC CCAAGACCGC AGTGCAGCCA
    GCAGTGTGCC AGGTGATCGG GATGTACGAT TACACCGCCC AGAACGATGA CGAACTAGCC
    TTCAGCAAAG GCCAGATCAT CAACGTCCTC AACAAGGAGG ACCCGGACTG GTGGAAAGGA
    GAAGTCAGTG GGCAAGTTGG GCTCTTCCCA TCCAATTATG TAAAGCTGAC CACAGACATG
    GACCCCAGCC AGCAATGGTG CTCAGACCTG CATCTCTTAG ATATGCTGAC CCCGACTGAG
    AGGAAGCGGC AAGGCTACAT CCATGAACTC ATTGTCACGG AGGAGAACTA CGTGAACGAC
    TTGCAGCTGG TCACAGAGAT CTTTCAGAAA CCCCTGACGG AGTCTGAGCT GCTGACAGAA
    AAAGAGGTTG CTATGATTTT TGTTAACTGG AAGGAGCTGA TCATGTGTAA TATCAAACTG
    CTGAAAGCGC TGAGAGTCCG CAAGAAGATG TCTGGGGAGA AGATGCCGGT GAAGATGATT
    GGCGACATCC TGAGCGCCCA GCTGCCGCAC ATGCAGCCTT ACATCCGCTT CTGCAGCTGC
    CAGCTCAATG GGGCTGCCCT CATCCAGCAG AAGACGGACG AGGCTCCAGA CTTCAAGGAG
    TTCGTCAAAA GACTGGCAAT GGACCCTCGG TGCAAAGGAA TGCCTCTGTC CAGCTTTATA
    CTGAAGCCTA TGCAGCGTGT CACAAGATAC CCGCTGATCA TTAAAAACAT CCTGGAAAAC
    ACTCCCGAGA ACCATCCAGA CCACAGCCAC CTGAAGCATG CCCTGGAAAA GGCGGAGGAG
    CTATGCTCCC AGGTGAACGA GGGAGTTCGA GAGAAGGAGA ACTCAGACCG GCTGGAGTGG
    ATCCAAGCCC ACGTGCAGTG TGAAGGCCTT TCTGAGCAAC TGGTGTTCAA TTCAGTGACC
    AACTGCTTGG GACCACGCAA GTTTCTGCAC AGCGGGAAGC TCTACAAGGC CAAGAGCAAC
    AAAGAACTGT ATGGCTTCCT CTTCAACGAC TTCCTCCTGC TGACCCAAAT CACAAAGCCC
    TTAGGCTCTT CCGGCACCGA CAAAGTCTTC AGCCCCAAAT CTAACCTTCA GTATAAAATG
    TACAAAACGC CCATTTTCTT AAATGAGGTT CTAGTAAAAT TGCCCACGGA CCCTTCTGGA
    GATGAGCCTA TCTTCCACAT TTCCCACATC GACCGGGTCT ACACCCTCCG AGCAGAGAGC
    ATAAATGAGA GGACTGCCTG GGTGCAGAAA ATCAAGGCGG CGTCTGAGCT CTACATAGAG
    ACGGAGAAAA AGAAGCGAGA GAAGGCGTAC CTGGTCCGTT CCCAGCGGGC GACCGGTATT
    GGAAGGTTGA TGGTGAACGT GGTAGAAGGC ATTGAGCTGA AGCCCTGTCG GTCACATGGA
    AAGAGCAACC CGTACTGTGA GGTGACCATG GGCTCTCAGT GCCACATCAC CAAGACAATC
    CAGGACACGC TAAACCCCAA GTGGAATTCT AACTGCCAGT TCTTCATCAG AGACCTGGAG
    CAGGAGGTTC TCTGCATCAC AGTGTTTGAG AGGGACCAGT TCTCGCCTGA TGATTTTTTG
    GGTCGGACAG AGATCCGAGT GGCCGACATC AAGAAAGACC AGGGCTCCAA GGGGCCGGTT
    ACGAAGTGTC TCCTGCTGCA TGAGGTCCCC ACGGGAGAGA TTGTGGTCCG CCTTGACCTG
    CAGTTGTTTG ATGAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523000.1
    CDS1371..5951
    Translation

    Target ORF information:

    RefSeq Version XM_006522937.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522937.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGAGAAG TGTTGCAGAA GTACTTACAG CAGCGTCCAG CGTGCGCCCC TCCAGCAGCA 
    GAATGGGCTG TGCCTCAGTC ATCAAGGCTG AAATACAGGC AGTTATTCAA CAGCCACGAC
    AAAACTATGA GTGGACACTT AACAGGTCCC CAGGCAAGAA CTATTCTCAT GCAATCAAGT
    TTACCCCAGG CTCAGCTGGC TTCAATATGG AATCTTTCTG ACATTGATCA AGATGGAAAA
    CTCACTGCAG AAGAATTTAT CCTAGCTATG CACCTAATTG ATGTTGCCAT GTCTGGTCAG
    CCACTGCCGC CCGTCCTGCC TCCAGAATAC ATCCCTCCTT CCTTCAGAAG AGTTCGCTCC
    GGCAGTGGGA TGTCCGTCAT AAGCTCTTCT TCTGTGGATC AGAGGCTGCC TGAGGAGCCG
    TCGTCAGAGG ATGAGCAGCA GCCAGAGAAG AAACTGCCTG TGACATTTGA AGATAAGAAG
    CGGGAGAACT TCGAGCGAGG CAGTGTGGAG CTGGAGAAGC GCCGCCAAGC GCTCTTGGAG
    CAGCAGCGCA AAGAGCAGGA GCGGTTGGCT CAGCTGGAGC GCGCCGAGCA GGAGAGGAAA
    GAGCGGGAGC GCCAGGAGCA GGAGCGCAAG CGGCAGCTGG AGCTGGAGAA GCAGCTGGAG
    AAGCAGCGGG AGCTGGAGCG GCAGCGAGAG GAGGAGAGGA GGAAGGAGAT CGAGAGGCGC
    GAGGCCGCAA AACGGGAACT GGAAAGGCAG CGACAACTTG AATGGGAACG GAACCGGAGA
    CAGGAACTCC TGAATCAGAG GAACAAGGAG CAGGAGGGCA CCGTGGTCCT GAAGGCAAGG
    AGGAAGACTC TGGAGTTTGA GTTAGAAGCT CTGAATGACA AAAAGCATCA GCTAGAAGGA
    AAACTTCAGG ATATCAGGTG TCGACTGGCA ACCCAGAGGC AAGAAATTGA GAGCACGAAC
    AAGTCTAGAG AGCTAAGAAT TGCTGAAATC ACCCACTTAC AGCAGCAGTT GCAGGAATCT
    CAGCAAATGC TTGGAAGACT TATTCCAGAG AAACAGATAC TCAGTGACCA GTTAAAACAA
    GTCCAGCAGA ACAGTTTGCA TAGAGACTCG CTTCTTACCC TCAAAAGAGC CTTGGAAGCA
    AAGGAGCTGG CCCGGCAGCA GCTCCGGGAG CAGCTGGACG AGGTGGAGAG AGAGACCAGG
    TCAAAGCTGC AGGAGATTGA TGTTTTCAAC AACCAGCTGA AGGAACTGAG AGAGATACAT
    AGCAAACAGC AACTCCAGAA GCAGAGGTCC CTGGAGGCAG CGCGACTGAA GCAGAAAGAG
    CAGGAGAGGA AGAGCCTGGA GTTAGAGAAG CAAAAGGAAG ACGCTCAGAG ACGAGTTCAG
    GAAAGGGACA AGCAATGGCT GGAGCATGTG CAGCAGGAGG AGCAGCCACG CCCCCGGAAA
    CCCCACGAGG AGGACAGACT GAAGAGGGAA GACAGTGTCA GGAAGAAGGA GGCGGAAGAG
    AGAGCCAAGC CGGAAATGCA AGACAAGCAG AGTCGGCTTT TCCATCCGCA TCAGGAGCCA
    GCTAAGCTGG CCACCCAGGC ACCCTGGTCT ACCACAGAGA AAGGCCCGCT TACCATTTCT
    GCACAGGAGA GTGTAAAAGT GGTATATTAC CGAGCGCTGT ACCCCTTTGA ATCCAGAAGT
    CACGATGAGA TCACCATCCA GCCAGGAGAT ATAGTCATGG TTAAAGGGGA ATGGGTGGAT
    GAAAGCCAGA CTGGAGAGCC AGGATGGCTT GGAGGAGAGC TGAAAGGGAA GACGGGATGG
    TTCCCTGCAA ACTATGCAGA AAAGATTCCA GAAAATGAGG TTCCCACTCC AGCCAAACCA
    GTGACCGATC TGACATCTGC CCCTGCCCCC AAACTGGCTC TGCGTGAGAC CCCTGCTCCT
    TTGCCAGTGA CCTCTTCTGA GCCCTCCACA ACCCCCAACA ACTGGGCAGA CTTCAGTTCC
    ACGTGGCCCA GCAGCTCAAA CGAGAAGCCA GAAACGGACA ACTGGGATAC ATGGGCGGCT
    CAGCCTTCTC TGACCGTACC TAGTGCTGGC CAGTTACGGC AGAGATCAGC CTTTACCCCA
    GCCACAGCCA CTGGCTCCTC CCCATCTCCC GTCCTGGGCC AGGGTGAAAA GGTGGAAGGG
    CTACAAGCGC AAGCCCTGTA TCCCTGGAGA GCCAAAAAAG ACAACCACTT AAATTTTAAC
    AAAAGTGACG TCATCACCGT TCTGGAACAG CAAGACATGT GGTGGTTTGG AGAAGTTCAA
    GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC GTGAAACTCA TTTCAGGGCC CGTAAGGAAA
    TCCACAAGCA TCGATACTGG CCCTACTGAA AGTCCTGCTA GTCTAAAGAG AGTGGCTTCC
    CCGGCCGCCA AGCCAGCCAT TCCCGGAGAA GAGTTTATTG CCATGTACAC ATACGAGAGT
    TCTGAGCAAG GAGATTTAAC CTTTCAGCAA GGGGATGTGA TTGTGGTTAC CAAGAAAGAT
    GGTGACTGGT GGACGGGAAC GGTGGGCGAC AAGTCCGGAG TCTTCCCTTC TAACTATGTG
    AGGCTTAAAG ATTCAGAGGG CTCTGGAACT GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA
    CCTGAAATTG CCCAGGTTAT TGCTTCCTAC GCTGCTACTG GTCCCGAACA ACTCACCCTG
    GCTCCTGGGC AGCTGATTCT GATCCGGAAA AAGAACCCAG GTGGATGGTG GGAAGGAGAA
    CTGCAAGCTC GAGGGAAAAA GCGCCAGATA GGGTGGTTTC CAGCAAATTA TGTCAAACTT
    CTAAGCCCCG GAACAAGCAA AATCACCCCA ACTGAGCTAC CCAAGACCGC AGTGCAGCCA
    GCAGTGTGCC AGGTGATCGG GATGTACGAT TACACCGCCC AGAACGATGA CGAACTAGCC
    TTCAGCAAAG GCCAGATCAT CAACGTCCTC AACAAGGAGG ACCCGGACTG GTGGAAAGGA
    GAAGTCAGTG GGCAAGTTGG GCTCTTCCCA TCCAATTATG TAAAGCTGAC CACAGACATG
    GACCCCAGCC AGCAATGGTG CTCAGACCTG CATCTCTTAG ATATGCTGAC CCCGACTGAG
    AGGAAGCGGC AAGGCTACAT CCATGAACTC ATTGTCACGG AGGAGAACTA CGTGAACGAC
    TTGCAGCTGG TCACAGAGAT CTTTCAGAAA CCCCTGACGG AGTCTGAGCT GCTGACAGAA
    AAAGAGGTTG CTATGATTTT TGTTAACTGG AAGGAGCTGA TCATGTGTAA TATCAAACTG
    CTGAAAGCGC TGAGAGTCCG CAAGAAGATG TCTGGGGAGA AGATGCCGGT GAAGATGATT
    GGCGACATCC TGAGCGCCCA GCTGCCGCAC ATGCAGCCTT ACATCCGCTT CTGCAGCTGC
    CAGCTCAATG GGGCTGCCCT CATCCAGCAG AAGACGGACG AGGCTCCAGA CTTCAAGGAG
    TTCGTCAAAA GACTGGCAAT GGACCCTCGG TGCAAAGGAA TGCCTCTGTC CAGCTTTATA
    CTGAAGCCTA TGCAGCGTGT CACAAGATAC CCGCTGATCA TTAAAAACAT CCTGGAAAAC
    ACTCCCGAGA ACCATCCAGA CCACAGCCAC CTGAAGCATG CCCTGGAAAA GGCGGAGGAG
    CTATGCTCCC AGGTGAACGA GGGAGTTCGA GAGAAGGAGA ACTCAGACCG GCTGGAGTGG
    ATCCAAGCCC ACGTGCAGTG TGAAGGCCTT TCTGAGCAAC TGGTGTTCAA TTCAGTGACC
    AACTGCTTGG GACCACGCAA GTTTCTGCAC AGCGGGAAGC TCTACAAGGC CAAGAGCAAC
    AAAGAACTGT ATGGCTTCCT CTTCAACGAC TTCCTCCTGC TGACCCAAAT CACAAAGCCC
    TTAGGCTCTT CCGGCACCGA CAAAGTCTTC AGCCCCAAAT CTAACCTTCA GTATAAAATG
    TACAAAACGC CCATTTTCTT AAATGAGGTT CTAGTAAAAT TGCCCACGGA CCCTTCTGGA
    GATGAGCCTA TCTTCCACAT TTCCCACATC GACCGGGTCT ACACCCTCCG AGCAGAGAGC
    ATAAATGAGA GGACTGCCTG GGTGCAGAAA ATCAAGGCGG CGTCTGAGCT CTACATAGAG
    ACGGAGAAAA AGAAGCGAGA GAAGGCGTAC CTGGTCCGTT CCCAGCGGGC GACCGGTATT
    GGAAGGTTGA TGGTGAACGT GGTAGAAGGC ATTGAGCTGA AGCCCTGTCG GTCACATGGA
    AAGAGCAACC CGTACTGTGA GGTGACCATG GGCTCTCAGT GCCACATCAC CAAGACAATC
    CAGGACACGC TAAACCCCAA GTGGAATTCT AACTGCCAGT TCTTCATCAG AGACCTGGAG
    CAGGAGGTTC TCTGCATCAC AGTGTTTGAG AGGGACCAGT TCTCGCCTGA TGATTTTTTG
    GGTCGGACAG AGATCCGAGT GGCCGACATC AAGAAAGACC AGGGCTCCAA GGGGCCGGTT
    ACGAAGTGTC TCCTGCTGCA TGAGGTCCCC ACGGGAGAGA TTGTGGTCCG CCTTGACCTG
    CAGTTGTTTG ATGAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52690
    Clone ID Related Accession (Same CDS sequence) XM_006522938.2
    Accession Version XM_006522938.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGGGTGAAA AGGTGGAAGG GCTACAAGCG
    CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA GACAACCACT TAAATTTTAA CAAAAGTGAC
    GTCATCACCG TTCTGGAACA GCAAGACATG TGGTGGTTTG GAGAAGTTCA AGGTCAGAAG
    GGTTGGTTCC CCAAGTCTTA CGTGAAACTC ATTTCAGGGC CCGTAAGGAA ATCCACAAGC
    ATCGATACTG GCCCTACTGA AAGTCCTGCT AGTCTAAAGA GAGTGGCTTC CCCGGCCGCC
    AAGCCAGCCA TTCCCGGAGA AGAGTTTATT GCCATGTACA CATACGAGAG TTCTGAGCAA
    GGAGATTTAA CCTTTCAGCA AGGGGATGTG ATTGTGGTTA CCAAGAAAGA TGGTGACTGG
    TGGACGGGAA CGGTGGGCGA CAAGTCCGGA GTCTTCCCTT CTAACTATGT GAGGCTTAAA
    GATTCAGAGG GCTCTGGAAC TGCTGGGAAA ACAGGGAGTT TAGGAAAAAA ACCTGAAATT
    GCCCAGGTTA TTGCTTCCTA CGCTGCTACT GGTCCCGAAC AACTCACCCT GGCTCCTGGG
    CAGCTGATTC TGATCCGGAA AAAGAACCCA GGTGGATGGT GGGAAGGAGA ACTGCAAGCT
    CGAGGGAAAA AGCGCCAGAT AGGGTGGTTT CCAGCAAATT ATGTCAAACT TCTAAGCCCC
    GGAACAAGCA AAATCACCCC AACTGAGCTA CCCAAGACCG CAGTGCAGCC AGCAGTGTGC
    CAGGTGATCG GGATGTACGA TTACACCGCC CAGAACGATG ACGAACTAGC CTTCAGCAAA
    GGCCAGATCA TCAACGTCCT CAACAAGGAG GACCCGGACT GGTGGAAAGG AGAAGTCAGT
    GGGCAAGTTG GGCTCTTCCC ATCCAATTAT GTAAAGCTGA CCACAGACAT GGACCCCAGC
    CAGCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523001.1
    CDS428..4156
    Translation

    Target ORF information:

    RefSeq Version XM_006522938.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522938.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGGGTGAAA AGGTGGAAGG GCTACAAGCG
    CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA GACAACCACT TAAATTTTAA CAAAAGTGAC
    GTCATCACCG TTCTGGAACA GCAAGACATG TGGTGGTTTG GAGAAGTTCA AGGTCAGAAG
    GGTTGGTTCC CCAAGTCTTA CGTGAAACTC ATTTCAGGGC CCGTAAGGAA ATCCACAAGC
    ATCGATACTG GCCCTACTGA AAGTCCTGCT AGTCTAAAGA GAGTGGCTTC CCCGGCCGCC
    AAGCCAGCCA TTCCCGGAGA AGAGTTTATT GCCATGTACA CATACGAGAG TTCTGAGCAA
    GGAGATTTAA CCTTTCAGCA AGGGGATGTG ATTGTGGTTA CCAAGAAAGA TGGTGACTGG
    TGGACGGGAA CGGTGGGCGA CAAGTCCGGA GTCTTCCCTT CTAACTATGT GAGGCTTAAA
    GATTCAGAGG GCTCTGGAAC TGCTGGGAAA ACAGGGAGTT TAGGAAAAAA ACCTGAAATT
    GCCCAGGTTA TTGCTTCCTA CGCTGCTACT GGTCCCGAAC AACTCACCCT GGCTCCTGGG
    CAGCTGATTC TGATCCGGAA AAAGAACCCA GGTGGATGGT GGGAAGGAGA ACTGCAAGCT
    CGAGGGAAAA AGCGCCAGAT AGGGTGGTTT CCAGCAAATT ATGTCAAACT TCTAAGCCCC
    GGAACAAGCA AAATCACCCC AACTGAGCTA CCCAAGACCG CAGTGCAGCC AGCAGTGTGC
    CAGGTGATCG GGATGTACGA TTACACCGCC CAGAACGATG ACGAACTAGC CTTCAGCAAA
    GGCCAGATCA TCAACGTCCT CAACAAGGAG GACCCGGACT GGTGGAAAGG AGAAGTCAGT
    GGGCAAGTTG GGCTCTTCCC ATCCAATTAT GTAAAGCTGA CCACAGACAT GGACCCCAGC
    CAGCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52691
    Clone ID Related Accession (Same CDS sequence) XM_006522939.2
    Accession Version XM_006522939.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTGGATGAAA GCCAGACTGG AGAGCCAGGA
    TGGCTTGGAG GAGAGCTGAA AGGGAAGACG GGATGGTTCC CTGCAAACTA TGCAGAAAAG
    ATTCCAGAAA ATGAGGTTCC CACTCCAGCC AAACCAGTGA CCGATCTGAC ATCTGCCCCT
    GCCCCCAAAC TGGCTCTGCG TGAGACCCCT GCTCCTTTGC CAGTGACCTC TTCTGAGCCC
    TCCACAACCC CCAACAACTG GGCAGACTTC AGTTCCACGT GGCCCAGCAG CTCAAACGAG
    AAGCCAGAAA CGGACAACTG GGATACATGG GCGGCTCAGC CTTCTCTGAC CGTACCTAGT
    GCTGGCCAGT TACGGCAGAG ATCAGCCTTT ACCCCAGCCA CAGCCACTGG CTCCTCCCCA
    TCTCCCGTCC TGGGCCAGGG TGAAAAGGTG GAAGGGCTAC AAGCGCAAGC CCTGTATCCC
    TGGAGAGCCA AAAAAGACAA CCACTTAAAT TTTAACAAAA GTGACGTCAT CACCGTTCTG
    GAACAGCAAG ACATGTGGTG GTTTGGAGAA GTTCAAGGTC AGAAGGGTTG GTTCCCCAAG
    TCTTACGTGA AACTCATTTC AGGGCCCGTA AGGAAATCCA CAAGCATCGA TACTGGCCCT
    ACTGAAAGTC CTGCTAGTCT AAAGAGAGTG GCTTCCCCGG CCGCCAAGCC AGCCATTCCC
    GGAGAAGAGT TTATTGCCAT GTACACATAC GAGAGTTCTG AGCAAGGAGA TTTAACCTTT
    CAGCAAGGGG ATGTGATTGT GGTTACCAAG AAAGATGGTG ACTGGTGGAC GGGAACGGTG
    GGCGACAAGT CCGGAGTCTT CCCTTCTAAC TATGTGAGGC TTAAAGATTC AGAGGGCTCT
    GGAACTGCTG GGAAAACAGG GAGTTTAGGA AAAAAACCTG AAATTGCCCA GGTTATTGCT
    TCCTACGCTG CTACTGGTCC CGAACAACTC ACCCTGGCTC CTGGGCAGCT GATTCTGATC
    CGGAAAAAGA ACCCAGGTGG ATGGTGGGAA GGAGAACTGC AAGCTCGAGG GAAAAAGCGC
    CAGATAGGGT GGTTTCCAGC AAATTATGTC AAACTTCTAA GCCCCGGAAC AAGCAAAATC
    ACCCCAACTG AGCTACCCAA GACCGCAGTG CAGCCAGCAG TGTGCCAGGT GATCGGGATG
    TACGATTACA CCGCCCAGAA CGATGACGAA CTAGCCTTCA GCAAAGGCCA GATCATCAAC
    GTCCTCAACA AGGAGGACCC GGACTGGTGG AAAGGAGAAG TCAGTGGGCA AGTTGGGCTC
    TTCCCATCCA ATTATGTAAA GCTGACCACA GACATGGACC CCAGCCAGCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523002.1
    CDS428..4141
    Translation

    Target ORF information:

    RefSeq Version XM_006522939.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522939.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTGGATGAAA GCCAGACTGG AGAGCCAGGA
    TGGCTTGGAG GAGAGCTGAA AGGGAAGACG GGATGGTTCC CTGCAAACTA TGCAGAAAAG
    ATTCCAGAAA ATGAGGTTCC CACTCCAGCC AAACCAGTGA CCGATCTGAC ATCTGCCCCT
    GCCCCCAAAC TGGCTCTGCG TGAGACCCCT GCTCCTTTGC CAGTGACCTC TTCTGAGCCC
    TCCACAACCC CCAACAACTG GGCAGACTTC AGTTCCACGT GGCCCAGCAG CTCAAACGAG
    AAGCCAGAAA CGGACAACTG GGATACATGG GCGGCTCAGC CTTCTCTGAC CGTACCTAGT
    GCTGGCCAGT TACGGCAGAG ATCAGCCTTT ACCCCAGCCA CAGCCACTGG CTCCTCCCCA
    TCTCCCGTCC TGGGCCAGGG TGAAAAGGTG GAAGGGCTAC AAGCGCAAGC CCTGTATCCC
    TGGAGAGCCA AAAAAGACAA CCACTTAAAT TTTAACAAAA GTGACGTCAT CACCGTTCTG
    GAACAGCAAG ACATGTGGTG GTTTGGAGAA GTTCAAGGTC AGAAGGGTTG GTTCCCCAAG
    TCTTACGTGA AACTCATTTC AGGGCCCGTA AGGAAATCCA CAAGCATCGA TACTGGCCCT
    ACTGAAAGTC CTGCTAGTCT AAAGAGAGTG GCTTCCCCGG CCGCCAAGCC AGCCATTCCC
    GGAGAAGAGT TTATTGCCAT GTACACATAC GAGAGTTCTG AGCAAGGAGA TTTAACCTTT
    CAGCAAGGGG ATGTGATTGT GGTTACCAAG AAAGATGGTG ACTGGTGGAC GGGAACGGTG
    GGCGACAAGT CCGGAGTCTT CCCTTCTAAC TATGTGAGGC TTAAAGATTC AGAGGGCTCT
    GGAACTGCTG GGAAAACAGG GAGTTTAGGA AAAAAACCTG AAATTGCCCA GGTTATTGCT
    TCCTACGCTG CTACTGGTCC CGAACAACTC ACCCTGGCTC CTGGGCAGCT GATTCTGATC
    CGGAAAAAGA ACCCAGGTGG ATGGTGGGAA GGAGAACTGC AAGCTCGAGG GAAAAAGCGC
    CAGATAGGGT GGTTTCCAGC AAATTATGTC AAACTTCTAA GCCCCGGAAC AAGCAAAATC
    ACCCCAACTG AGCTACCCAA GACCGCAGTG CAGCCAGCAG TGTGCCAGGT GATCGGGATG
    TACGATTACA CCGCCCAGAA CGATGACGAA CTAGCCTTCA GCAAAGGCCA GATCATCAAC
    GTCCTCAACA AGGAGGACCC GGACTGGTGG AAAGGAGAAG TCAGTGGGCA AGTTGGGCTC
    TTCCCATCCA ATTATGTAAA GCTGACCACA GACATGGACC CCAGCCAGCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52692
    Clone ID Related Accession (Same CDS sequence) XM_006522940.2
    Accession Version XM_006522940.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522940.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGCCATGTC TCCTGCTCCG CCCCTTCCTG
    AGCCGAGGCT GCCCACAGCC CCGGAGCCTG CTCCTCCTCC TGGACCACGT GCTGTGCACT
    CAGCGTGCCA CCTCAGGGCC TTTGCCTGTG CTGTGCCTTT GGCCTGGAGC GCCCTTCTGC
    CTCCCTAGCT CTCCAAACCC TTCAAGGCCC AGCTCAAGTG CCTCCTCCCC TGTGAAACTG
    TCCCTGGCGT CCCGAGGCAG GGGTTGGCCT TTTCTCTCTG GTCCTGCTGG GAAGCAGAAG
    TTTTGTATAG TGTGTTTCAC TCCACCCAAT GATACACGCG GCTGTTTCTA TCTGTTCCTT
    GCACACAAAG TTCCTCACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523003.1
    CDS428..3568
    Translation

    Target ORF information:

    RefSeq Version XM_006522940.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522940.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGCCATGTC TCCTGCTCCG CCCCTTCCTG
    AGCCGAGGCT GCCCACAGCC CCGGAGCCTG CTCCTCCTCC TGGACCACGT GCTGTGCACT
    CAGCGTGCCA CCTCAGGGCC TTTGCCTGTG CTGTGCCTTT GGCCTGGAGC GCCCTTCTGC
    CTCCCTAGCT CTCCAAACCC TTCAAGGCCC AGCTCAAGTG CCTCCTCCCC TGTGAAACTG
    TCCCTGGCGT CCCGAGGCAG GGGTTGGCCT TTTCTCTCTG GTCCTGCTGG GAAGCAGAAG
    TTTTGTATAG TGTGTTTCAC TCCACCCAAT GATACACGCG GCTGTTTCTA TCTGTTCCTT
    GCACACAAAG TTCCTCACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu68032
    Clone ID Related Accession (Same CDS sequence) XM_011246102.2 , XM_011246102.3
    Accession Version XM_011246102.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product intersectin-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246102.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGGGTGAAA AGGTGGAAGG GCTACAAGCG
    CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA GACAACCACT TAAATTTTAA CAAAAGTGAC
    GTCATCACCG TTCTGGAACA GCAAGACATG TGGTGGTTTG GAGAAGTTCA AGCATCGATA
    CTGGCCCTAC TGAAAGTCCT GCTAGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011244404.1
    CDS428..3397
    Translation

    Target ORF information:

    RefSeq Version XM_011246102.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011246102.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGACTC TGGCCAGATG CGGTTCTTTT GTGTTTATCG GCGTCGATCA GCAAGGTGAA 
    CGTAATAGAA CCATGGCTCA GTTTCCCACA CCTTTCGGTG GTAGCCTGGA TGTCTGGGCC
    ATAACTGTGG AGGAAAGGGC CAAGCATGAC CAGCAGTTCC TTAGCCTGAA GCCGATAGCG
    GGATTTATTA CTGGTGATCA AGCGAGGAAC TTTTTTTTCC AATCTGGGTT ACCTCAGCCT
    GTCTTAGCAC AAATATGGGC GCTAGCGGAC ATGAATAACG ATGGAAGGAT GGATCAAGTG
    GAATTTTCCA TAGCCATGAA GCTTATCAAA CTGAAGCTAC AAGGATATCA GCTCCCCTCC
    ACACTTCCCC CTGTCATGAA ACAGCAACCA GTGGCTATTT CCAGTGCACC AGCATTTGGT
    ATAGGAGGGA TTGCTAGCAT GCCACCACTC ACAGCTGTTG CTCCTGTGCC AATGGGCTCC
    ATTCCAGTTG TTGGAATGTC TCCACCCTTA GTATCTTCTG TCCCTCCAGC AGCAGTGCCT
    CCCCTGGCTA ACGGGGCTCC TCCCGTCATA CAGCCTCTGC CTGCGTTTGC GCATCCTGCA
    GCCACATTGC CAAAGAGTTC TTCCTTCAGC AGATCTGGTC CAGGGTCACA ATTAAACACT
    AAGTTACAGA AGGCACAATC ATTCGATGTC GCCAGCGCCC CTCCAGCAGC AGAATGGGCT
    GTGCCTCAGT CATCAAGGCT GAAATACAGG CAGTTATTCA ACAGCCACGA CAAAACTATG
    AGTGGACACT TAACAGGTCC CCAGGCAAGA ACTATTCTCA TGCAATCAAG TTTACCCCAG
    GCTCAGCTGG CTTCAATATG GAATCTTTCT GACATTGATC AAGATGGAAA ACTCACTGCA
    GAAGAATTTA TCCTAGCTAT GCACCTAATT GATGTTGCCA TGTCTGGTCA GCCACTGCCG
    CCCGTCCTGC CTCCAGAATA CATCCCTCCT TCCTTCAGAA GAGTTCGCTC CGGCAGTGGG
    ATGTCCGTCA TAAGCTCTTC TTCTGTGGAT CAGAGGCTGC CTGAGGAGCC GTCGTCAGAG
    GATGAGCAGC AGCCAGAGAA GAAACTGCCT GTGACATTTG AAGATAAGAA GCGGGAGAAC
    TTCGAGCGAG GCAGTGTGGA GCTGGAGAAG CGCCGCCAAG CGCTCTTGGA GCAGCAGCGC
    AAAGAGCAGG AGCGGTTGGC TCAGCTGGAG CGCGCCGAGC AGGAGAGGAA AGAGCGGGAG
    CGCCAGGAGC AGGAGCGCAA GCGGCAGCTG GAGCTGGAGA AGCAGCTGGA GAAGCAGCGG
    GAGCTGGAGC GGCAGCGAGA GGAGGAGAGG AGGAAGGAGA TCGAGAGGCG CGAGGCCGCA
    AAACGGGAAC TGGAAAGGCA GCGACAACTT GAATGGGAAC GGAACCGGAG ACAGGAACTC
    CTGAATCAGA GGAACAAGGA GCAGGAGGGC ACCGTGGTCC TGAAGGCAAG GAGGAAGACT
    CTGGAGTTTG AGTTAGAAGC TCTGAATGAC AAAAAGCATC AGCTAGAAGG AAAACTTCAG
    GATATCAGGT GTCGACTGGC AACCCAGAGG CAAGAAATTG AGAGCACGAA CAAGTCTAGA
    GAGCTAAGAA TTGCTGAAAT CACCCACTTA CAGCAGCAGT TGCAGGAATC TCAGCAAATG
    CTTGGAAGAC TTATTCCAGA GAAACAGATA CTCAGTGACC AGTTAAAACA AGTCCAGCAG
    AACAGTTTGC ATAGAGACTC GCTTCTTACC CTCAAAAGAG CCTTGGAAGC AAAGGAGCTG
    GCCCGGCAGC AGCTCCGGGA GCAGCTGGAC GAGGTGGAGA GAGAGACCAG GTCAAAGCTG
    CAGGAGATTG ATGTTTTCAA CAACCAGCTG AAGGAACTGA GAGAGATACA TAGCAAACAG
    CAACTCCAGA AGCAGAGGTC CCTGGAGGCA GCGCGACTGA AGCAGAAAGA GCAGGAGAGG
    AAGAGCCTGG AGTTAGAGAA GCAAAAGGAA GACGCTCAGA GACGAGTTCA GGAAAGGGAC
    AAGCAATGGC TGGAGCATGT GCAGCAGGAG GAGCAGCCAC GCCCCCGGAA ACCCCACGAG
    GAGGACAGAC TGAAGAGGGA AGACAGTGTC AGGAAGAAGG AGGCGGAAGA GAGAGCCAAG
    CCGGAAATGC AAGACAAGCA GAGTCGGCTT TTCCATCCGC ATCAGGAGCC AGCTAAGCTG
    GCCACCCAGG CACCCTGGTC TACCACAGAG AAAGGCCCGC TTACCATTTC TGCACAGGAG
    AGTGTAAAAG TGGTATATTA CCGAGCGCTG TACCCCTTTG AATCCAGAAG TCACGATGAG
    ATCACCATCC AGCCAGGAGA TATAGTCATG GTTAAAGGGG AATGGGTGGA TGAAAGCCAG
    ACTGGAGAGC CAGGATGGCT TGGAGGAGAG CTGAAAGGGA AGACGGGATG GTTCCCTGCA
    AACTATGCAG AAAAGATTCC AGAAAATGAG GTTCCCACTC CAGCCAAACC AGTGACCGAT
    CTGACATCTG CCCCTGCCCC CAAACTGGCT CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG
    ACCTCTTCTG AGCCCTCCAC AACCCCCAAC AACTGGGCAG ACTTCAGTTC CACGTGGCCC
    AGCAGCTCAA ACGAGAAGCC AGAAACGGAC AACTGGGATA CATGGGCGGC TCAGCCTTCT
    CTGACCGTAC CTAGTGCTGG CCAGTTACGG CAGAGATCAG CCTTTACCCC AGCCACAGCC
    ACTGGCTCCT CCCCATCTCC CGTCCTGGGC CAGGGTGAAA AGGTGGAAGG GCTACAAGCG
    CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA GACAACCACT TAAATTTTAA CAAAAGTGAC
    GTCATCACCG TTCTGGAACA GCAAGACATG TGGTGGTTTG GAGAAGTTCA AGCATCGATA
    CTGGCCCTAC TGAAAGTCCT GCTAGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.