Itsn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itsn1
Entrez Gene ID	29491
Full Name	intersectin 1
Synonyms	EHSH1,Itsn
General protein information	Preferred Names intersectin 1 Names intersectin-1 EH domain and SH3 domain regulator of endocytosis 1 intersectin 1 (SH3 domain protein 1A) intersectin 1 (SH3 domain protein)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q11
Summary	binds to dynamin and SNAP-25; may be involved in exocytosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001136096.1	NP_001129568.1	intersectin-1 isoform a
NM_019227.1	NP_062100.1	intersectin-1 isoform b

Pathways from BioSystems

Homologies

Homo sapiens (human)	ITSN1	NP_003015.2
Canis lupus familiaris (dog)	ITSN1	XP_005638937.1
Drosophila melanogaster (fruit fly)	Dap160	NP_001246118.1
Xenopus tropicalis (tropical clawed frog)	itsn1	XP_002939390.1
Bos taurus (cattle)	ITSN1	XP_002707731.1
Mus musculus (house mouse)	Itsn1	NP_034717.2
Gallus gallus (chicken)	ITSN1	XP_416715.2
	ITSN1	XP_002803192.1
Rattus norvegicus (Norway rat)	Itsn1	NP_001129568.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009037	XP_319789.4
Pan troglodytes (chimpanzee)	ITSN1	XP_001166935.1
Canis lupus familiaris (dog)	LOC100856635	XP_003640127.1
Danio rerio (zebrafish)	itsn1	NP_997065.1
Caenorhabditis elegans (roundworm)	itsn-1	NP_001255927.1

Gene Ontology
Bibliography

Related articles in PubMed

Altered Expression of Intersectin1-L in Patients with Refractory Epilepsy and in Experimental Epileptic Rats.
Yang X, Xu X, Zhang Y, Wang S, Li M, Wang X
Cellular and molecular neurobiology35(6)871-80(2015 Aug)

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kr?hnert K, Sch?fer C, Rammner B, Koo SJ, Cla?en GA, Krauss M, Haucke V, Urlaub H, Rizzoli SO
Science (New York, N.Y.)344(6187)1023-8(2014 May)

Fast neurotransmitter release regulated by the endocytic scaffold intersectin.
Sakaba T, Kononenko NL, Bacetic J, Pechstein A, Schmoranzer J, Yao L, Barth H, Shupliakov O, Kobler O, Aktories K, Haucke V
Proceedings of the National Academy of Sciences of the United States of America110(20)8266-71(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The intersectin1-L protein was primarily expressed in neurons in brain tissue. Its expression was remarkably increased in patients with refractory epilepsy and in epilepsy model rats.
Title: Altered Expression of Intersectin1-L in Patients with Refractory Epilepsy and in Experimental Epileptic Rats.

intersectin is an endocytic scaffold with a role in fast neurotransmitter release, which may be of prime importance for information processing in the brain
Title: Fast neurotransmitter release regulated by the endocytic scaffold intersectin.

Intersectin-1L and beta-Pix, play essential roles in neuroendocrine exocytosis by controlling the activity of Cdc42 and Rac1, respectively.
Title: The Rho guanine nucleotide exchange factors Intersectin 1L and ?-Pix control calcium-regulated exocytosis in neuroendocrine PC12 cells.

intersectin-l is indeed an important regulator of constitutive endocytosis and neuronal development but that it is not a prominent player in the regulated endocytosis of SVs.
Title: Intersectin regulates dendritic spine development and somatodendritic endocytosis but not synaptic vesicle recycling in hippocampal neurons.

These results highlight a role of Numb for dendritic spine development and synaptic functions with intersectin and EphB2.
Title: Role of numb in dendritic spine development with a Cdc42 GEF intersectin and EphB2.

The following Itsn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itsn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04096	NM_019227.1 Latest version!	Rattus norvegicus intersectin 1 (Itsn1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa04191	NM_001136096.1 Latest version!	Rattus norvegicus intersectin 1 (Itsn1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itsn1 ( NM_019227.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062100.1 Rattus norvegicus intersectin 1 (Itsn1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa04096
Clone ID Related Accession (Same CDS sequence)	NM_019227.1
Accession Version	NM_019227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	intersectin-1 isoform b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF127798.1. On or before Oct 23, 2008 this sequence version replaced XM_573259.2, XM_001059402.1, XM_001069789.1. Transcript Variant: This variant (2) lacks alternate exons in the 3' coding region and 3' UTR, compared to variant 1, resulting in a novel 3' coding region and 3' UTR. It encodes the shorter isoform (b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF127798.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGT TTCCGACACC TTTTGGTGGG AGCCTGGACA TCTGGGCCAT AACCGTGGAG 
GAAAGAGCCA AGCATGACCA GCAGTTCCAG AGTTTGAAGC CGATATCTGG ATTCATCACT 
GGTGATCAAG CAAGGAACTT CTTTTTCCAA TCTGGGTTAC CTCAGCCCGT CTTAGCACAA 
ATATGGGCGC TGGCTGACAT GAATAAGGAC GGAAGGATGG ATCAGGTGGA GTTTTCCATA 
GCCATGAAGC TCATCAAACT GAAGCTGCAA GGATATCAGC TCCCCCCCGC ACTTCCCCCT 
GTCATGAAGC AGCAGCCAGC GGCCATCTCT AGTGCACCAG CGTTTGGTAT AGGAGGGATG 
GCTGGAATGC CACCACTGAC AGCTGTTGCT CCCGTGCCAA TGGGCTCCAT CCCAGTTGTT 
GGAATGTCTC CGCCCTTAGT ATCTTCTGTC CCTCAAGCAG CAGTGCCTCC CCTGGCTAAC 
GGGGCCCCTC CTGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA ACTACAGAAG 
GCACAATCAT TTGATGTAGC CAGCGCCCCT GCAGCGGCAG AATGGGCTGT GCCTCAGTCG 
TCAAGACTGA AATACAGGCA GTTATTCAAC AGTCACGACA AGACCATGAG TGGACACTTA 
ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT 
TCAATCTGGA ATCTTTCCGA CATTGATCAA GATGGAAAGC TCACCGCAGA AGAATTCATC 
CTAGCGATGC ACCTGATTGA TGTTGCTATG TCTGGTCAGC CACTGCCGCC TGTCCTGCCT 
CCAGAATACA TCCCTCCTTC CTTTAGAAGA GTTCGCTCTG GCAGTGGGAT GTCCGTCATA 
AGCTCTTCGT CTGCAGACCA GCGGCTGCCC GAGGAGCCAT CGTCAGAGGA TGAGCAGCAG 
GTGGAAAAGA AGCTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC 
AACCTGGAGC TGGAGAAGCG CAGGCAGGCC CTCCTGGAGC AGCAGCGCAA GGAGCAGGAG 
CGGCTGGCTC AGCTGGAGCG CGCCGAGCAA GAGAGGAAAG AGCGGGAGCG CCAGGAACAG 
GAGCGCAAGA GACAGCTAGA GCTGGAGAAG CAGCTAGAAA AGCAGCGGGA GCTGGAGAGG 
CAGAGAGAGG AGGAGAGAAG GAAGGAGATC GAGAGGCGGG AGGCAGCAAA ACGGGAGCTC 
GAACGGCAGC GACAGCTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GACTCAGAGG 
AACAAGGACC AGGAGGGCAT CGTGGTTCTG AAGGCGAGGA GGAAGACTCT GGAGTTTGAA 
TTAGAAGCTC TGAATGACAA AAAGCATCAG CTGGAAGGAA AACTTCAGGA TATCAGGTGT 
CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACAAACA AGTCTAGAGA GCTGAGAATT 
GCGGAAATCA CCCACTTACA GCAACAGTTG CAGGAATCTC AGCAGATGCT TGGAAGACTT 
ATTCCAGAGA AGCAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGCTTGCAT 
AGAGATTCGC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAACTGGC CCGGCAGCAG 
CTTCGAGAGC AGCTGGACGA GGTGGAGAAA GAGACCAGGT CAAAGCTGCA GGAGATTGAT 
GTTTTCAACA ACCAGCTGAA GGAACTGAGA GAGATACACA GTAAACAGCA GCTCCAGAAG 
CAGAGGTCCA TCGAGGCGGA GAGGCTAAAG CAGAAAGAGC AGGAGAGGAA GAGCCTGGAG 
TTGGAGAAGC AAAAGGAAGA AGGTCAGAGA CGAGTTCAGG AAAGGGACAA GCAATGGCAG 
GAGCATGTCC AGCAGGAGGA GCAGCAGCGC CCTCGGAAAC CCCACGAGGA GGACAAACTG 
AAAAGGGAAG ACAGTGTCAA GAAGAAGGAG GCGGAAGAGA GAGCCAAGCC GGAAGTGCAA 
GACAAGCAGA GTCGGCTTTT CCATCCACAT CAAGAGCCAG CTAAGCCGGC GCAGGCACCC 
TGGCCCACGA CAGAGAAAGG TCCGCTTACA ATCTCTGCAC AGGAGAGTGC CAAAGTGGTG 
TATTACCGAG CGCTGTACCC CTTTGAGTCC AGAAGTCATG ACGAGATCAC CATCCAGCCA 
GGAGATATAG TCATGGTTAA AGGGGAATGG GTGGATGAAA GCCAGACCGG AGAGCCAGGA 
TGGCTTGGAG GAGAACCGAA GGGGAAGACA GGATGGTTCC CTGCAAACTA TGCAGAGAAG 
ATTCCAGAAA ATGAGATTCC CACTCCAGCC AAACCAGTGA CCGATCTGAC ATCTGCCCCT 
GCCCCCAAAC TGGCTCTGCG TGAGACCCCT GCTCCTTTGC CAGTGACCTC TTCTGAGCCC 
TCCACAACCC CCAACAACTG GGCAGACTTC AGTTCCACGT GGCCCAGCAG CACAAATGAG 
AAACCAGAAA CGGACAACTG GGACACGTGG GCGGCTCAGC CTTCTCTGAC TGTACCCAGT 
GCTGGCCAGT TAAGGCAGAG GTCGGCCTTC ACACCAGCCA CAGCCACTGG CTCCTCCCCT 
TCTCCCGTCC TGGGCCAGGG TGAAAAGGTG GAAGGGCTCC AAGCACAAGC CCTGTATCCC 
TGGAGAGCCA AAAAAGACAA CCACTTAAAT TTTAACAAAA GTGATGTCAT CACCGTTCTG 
GAACAGCAAG ACATGTGGTG GTTTGGAGAA GTTCAAGGTC AGAAGGGTTG GTTCCCCAAG 
TCTTACGTGA AACTCATTTC AGGGCCCGTA AGGAAATCCA CAAGCATCGA TACTGGCCCT 
ACGGAAGCTC CCTCTAGTCT AAAGAGAGTG GCTTCCCCAG CCGCCAAGCC AGCCATTCCT 
GGAGAAGAAT TTGTTGCTAT GTACACTTAC GAGAGTTCTG AGCATGGAGA TTTAACCTTT 
CAGCAAGGGC ATGTGATTGT GGTTACCAAG AAGGATGGTG ACTGGTGGAC GGGCACAGTG 
GGCGAGACGT CCGGAGTCTT CCCTTCTAAC TATGTGAGGC TTAAAGACTC AGAGGGCTCT 
GGAACTGCGG GGAAAACAGG GAGTTTAGAA AAAAAACCTG AAATCGCCCA GGTCATTGCT 
TCCTACACTG CTACGGGTCC TGAACAGCTC ACCCTGGCTC CTGGTCAGCT GATTTTGATC 
CGAAAAAAGA ACCCCGGTGG ATGGTGGGAA GGAGAACTAC AAGCTCGAGG GAAAAAGCGC 
CAGATAGGGT GGTTCCCAGC AAATTATGTC AAACTTCTAA GCCCTGGAAC AAGCAAAATC 
ACCCCAACTG AGCTACCCAA GACCGCAGTG CAGCCAGCAG TGTGCCAGGT GATCGGGATG 
TACGATTACA CCGCGCAGAA TGACGATGAG CTAGCCTTCA GCAAAGGCCA GATCATCAAC 
GTCCTCAGCA AGGAGGACCC GGACTGGTGG AAAGGAGAAG TCAGTGGGCA GGTTGGGCTC 
TTCCCGTCCA ATTATGTGAA GCTGACCACA GACATGGACC CCAGCCAGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062100.1
CDS	41..3694
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFQSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNKDGRMDQVEFSIAMKLIKLKLQGYQLPPALPPVMKQQPAAISSAPAFGIGGMAGMPPLTAVAPVPMGSIPVVGMSPPLVSSVPQAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASAPAAAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGMSVISSSSADQRLPEEPSSEDEQQVEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLTQRNKDQEGIVVLKARRKTLEFELEALNDKKHQLEGKLQDIRCRLATQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILSDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLREQLDEVEKETRSKLQEIDVFNNQLKELREIHSKQQLQKQRSIEAERLKQKEQERKSLELEKQKEEGQRRVQERDKQWQEHVQQEEQQRPRKPHEEDKLKREDSVKKKEAEERAKPEVQDKQSRLFHPHQEPAKPAQAPWPTTEKGPLTISAQESAKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGEPKGKTGWFPANYAEKIPENEIPTPAKPVTDLTSAPAPKLALRETPAPLPVTSSEPSTTPNNWADFSSTWPSSTNEKPETDNWDTWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKSDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPVRKSTSIDTGPTEAPSSLKRVASPAAKPAIPGEEFVAMYTYESSEHGDLTFQQGHVIVVTKKDGDWWTGTVGETSGVFPSNYVRLKDSEGSGTAGKTGSLEKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTELPKTAVQPAVCQVIGMYDYTAQNDDELAFSKGQIINVLSKEDPDWWKGEVSGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	NM_019227.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus intersectin 1 (Itsn1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019227.1

ORF Insert Sequence:

ATGGCTCAGT TTCCGACACC TTTTGGTGGG AGCCTGGACA TCTGGGCCAT AACCGTGGAG 
GAAAGAGCCA AGCATGACCA GCAGTTCCAG AGTTTGAAGC CGATATCTGG ATTCATCACT 
GGTGATCAAG CAAGGAACTT CTTTTTCCAA TCTGGGTTAC CTCAGCCCGT CTTAGCACAA 
ATATGGGCGC TGGCTGACAT GAATAAGGAC GGAAGGATGG ATCAGGTGGA GTTTTCCATA 
GCCATGAAGC TCATCAAACT GAAGCTGCAA GGATATCAGC TCCCCCCCGC ACTTCCCCCT 
GTCATGAAGC AGCAGCCAGC GGCCATCTCT AGTGCACCAG CGTTTGGTAT AGGAGGGATG 
GCTGGAATGC CACCACTGAC AGCTGTTGCT CCCGTGCCAA TGGGCTCCAT CCCAGTTGTT 
GGAATGTCTC CGCCCTTAGT ATCTTCTGTC CCTCAAGCAG CAGTGCCTCC CCTGGCTAAC 
GGGGCCCCTC CTGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA ACTACAGAAG 
GCACAATCAT TTGATGTAGC CAGCGCCCCT GCAGCGGCAG AATGGGCTGT GCCTCAGTCG 
TCAAGACTGA AATACAGGCA GTTATTCAAC AGTCACGACA AGACCATGAG TGGACACTTA 
ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT 
TCAATCTGGA ATCTTTCCGA CATTGATCAA GATGGAAAGC TCACCGCAGA AGAATTCATC 
CTAGCGATGC ACCTGATTGA TGTTGCTATG TCTGGTCAGC CACTGCCGCC TGTCCTGCCT 
CCAGAATACA TCCCTCCTTC CTTTAGAAGA GTTCGCTCTG GCAGTGGGAT GTCCGTCATA 
AGCTCTTCGT CTGCAGACCA GCGGCTGCCC GAGGAGCCAT CGTCAGAGGA TGAGCAGCAG 
GTGGAAAAGA AGCTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC 
AACCTGGAGC TGGAGAAGCG CAGGCAGGCC CTCCTGGAGC AGCAGCGCAA GGAGCAGGAG 
CGGCTGGCTC AGCTGGAGCG CGCCGAGCAA GAGAGGAAAG AGCGGGAGCG CCAGGAACAG 
GAGCGCAAGA GACAGCTAGA GCTGGAGAAG CAGCTAGAAA AGCAGCGGGA GCTGGAGAGG 
CAGAGAGAGG AGGAGAGAAG GAAGGAGATC GAGAGGCGGG AGGCAGCAAA ACGGGAGCTC 
GAACGGCAGC GACAGCTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GACTCAGAGG 
AACAAGGACC AGGAGGGCAT CGTGGTTCTG AAGGCGAGGA GGAAGACTCT GGAGTTTGAA 
TTAGAAGCTC TGAATGACAA AAAGCATCAG CTGGAAGGAA AACTTCAGGA TATCAGGTGT 
CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACAAACA AGTCTAGAGA GCTGAGAATT 
GCGGAAATCA CCCACTTACA GCAACAGTTG CAGGAATCTC AGCAGATGCT TGGAAGACTT 
ATTCCAGAGA AGCAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGCTTGCAT 
AGAGATTCGC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAACTGGC CCGGCAGCAG 
CTTCGAGAGC AGCTGGACGA GGTGGAGAAA GAGACCAGGT CAAAGCTGCA GGAGATTGAT 
GTTTTCAACA ACCAGCTGAA GGAACTGAGA GAGATACACA GTAAACAGCA GCTCCAGAAG 
CAGAGGTCCA TCGAGGCGGA GAGGCTAAAG CAGAAAGAGC AGGAGAGGAA GAGCCTGGAG 
TTGGAGAAGC AAAAGGAAGA AGGTCAGAGA CGAGTTCAGG AAAGGGACAA GCAATGGCAG 
GAGCATGTCC AGCAGGAGGA GCAGCAGCGC CCTCGGAAAC CCCACGAGGA GGACAAACTG 
AAAAGGGAAG ACAGTGTCAA GAAGAAGGAG GCGGAAGAGA GAGCCAAGCC GGAAGTGCAA 
GACAAGCAGA GTCGGCTTTT CCATCCACAT CAAGAGCCAG CTAAGCCGGC GCAGGCACCC 
TGGCCCACGA CAGAGAAAGG TCCGCTTACA ATCTCTGCAC AGGAGAGTGC CAAAGTGGTG 
TATTACCGAG CGCTGTACCC CTTTGAGTCC AGAAGTCATG ACGAGATCAC CATCCAGCCA 
GGAGATATAG TCATGGTTAA AGGGGAATGG GTGGATGAAA GCCAGACCGG AGAGCCAGGA 
TGGCTTGGAG GAGAACCGAA GGGGAAGACA GGATGGTTCC CTGCAAACTA TGCAGAGAAG 
ATTCCAGAAA ATGAGATTCC CACTCCAGCC AAACCAGTGA CCGATCTGAC ATCTGCCCCT 
GCCCCCAAAC TGGCTCTGCG TGAGACCCCT GCTCCTTTGC CAGTGACCTC TTCTGAGCCC 
TCCACAACCC CCAACAACTG GGCAGACTTC AGTTCCACGT GGCCCAGCAG CACAAATGAG 
AAACCAGAAA CGGACAACTG GGACACGTGG GCGGCTCAGC CTTCTCTGAC TGTACCCAGT 
GCTGGCCAGT TAAGGCAGAG GTCGGCCTTC ACACCAGCCA CAGCCACTGG CTCCTCCCCT 
TCTCCCGTCC TGGGCCAGGG TGAAAAGGTG GAAGGGCTCC AAGCACAAGC CCTGTATCCC 
TGGAGAGCCA AAAAAGACAA CCACTTAAAT TTTAACAAAA GTGATGTCAT CACCGTTCTG 
GAACAGCAAG ACATGTGGTG GTTTGGAGAA GTTCAAGGTC AGAAGGGTTG GTTCCCCAAG 
TCTTACGTGA AACTCATTTC AGGGCCCGTA AGGAAATCCA CAAGCATCGA TACTGGCCCT 
ACGGAAGCTC CCTCTAGTCT AAAGAGAGTG GCTTCCCCAG CCGCCAAGCC AGCCATTCCT 
GGAGAAGAAT TTGTTGCTAT GTACACTTAC GAGAGTTCTG AGCATGGAGA TTTAACCTTT 
CAGCAAGGGC ATGTGATTGT GGTTACCAAG AAGGATGGTG ACTGGTGGAC GGGCACAGTG 
GGCGAGACGT CCGGAGTCTT CCCTTCTAAC TATGTGAGGC TTAAAGACTC AGAGGGCTCT 
GGAACTGCGG GGAAAACAGG GAGTTTAGAA AAAAAACCTG AAATCGCCCA GGTCATTGCT 
TCCTACACTG CTACGGGTCC TGAACAGCTC ACCCTGGCTC CTGGTCAGCT GATTTTGATC 
CGAAAAAAGA ACCCCGGTGG ATGGTGGGAA GGAGAACTAC AAGCTCGAGG GAAAAAGCGC 
CAGATAGGGT GGTTCCCAGC AAATTATGTC AAACTTCTAA GCCCTGGAAC AAGCAAAATC 
ACCCCAACTG AGCTACCCAA GACCGCAGTG CAGCCAGCAG TGTGCCAGGT GATCGGGATG 
TACGATTACA CCGCGCAGAA TGACGATGAG CTAGCCTTCA GCAAAGGCCA GATCATCAAC 
GTCCTCAGCA AGGAGGACCC GGACTGGTGG AAAGGAGAAG TCAGTGGGCA GGTTGGGCTC 
TTCCCGTCCA ATTATGTGAA GCTGACCACA GACATGGACC CCAGCCAGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn1 ( NM_001136096.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001129568.1 Rattus norvegicus intersectin 1 (Itsn1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa04191
Clone ID Related Accession (Same CDS sequence)	NM_001136096.1
Accession Version	NM_001136096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	intersectin-1 isoform a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473989.1. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGT TTCCGACACC TTTTGGTGGG AGCCTGGACA TCTGGGCCAT AACCGTGGAG 
GAAAGAGCCA AGCATGACCA GCAGTTCCAG AGTTTGAAGC CGATATCTGG ATTCATCACT 
GGTGATCAAG CAAGGAACTT CTTTTTCCAA TCTGGGTTAC CTCAGCCCGT CTTAGCACAA 
ATATGGGCGC TGGCTGACAT GAATAAGGAC GGAAGGATGG ATCAGGTGGA GTTTTCCATA 
GCCATGAAGC TCATCAAACT GAAGCTGCAA GGATATCAGC TCCCCCCCGC ACTTCCCCCT 
GTCATGAAGC AGCAGCCAGC GGCCATCTCT AGTGCACCAG CGTTTGGTAT AGGAGGGATG 
GCTGGAATGC CACCACTGAC AGCTGTTGCT CCCGTGCCAA TGGGCTCCAT CCCAGTTGTT 
GGAATGTCTC CGCCCTTAGT ATCTTCTGTC CCTCAAGCAG CAGTGCCTCC CCTGGCTAAC 
GGGGCCCCTC CTGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA ACTACAGAAG 
GCACAATCAT TTGATGTAGC CAGCGCCCCT GCAGCGGCAG AATGGGCTGT GCCTCAGTCG 
TCAAGACTGA AATACAGGCA GTTATTCAAC AGTCACGACA AGACCATGAG TGGACACTTA 
ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT 
TCAATCTGGA ATCTTTCCGA CATTGATCAA GATGGAAAGC TCACCGCAGA AGAATTCATC 
CTAGCGATGC ACCTGATTGA TGTTGCTATG TCTGGTCAGC CACTGCCGCC TGTCCTGCCT 
CCAGAATACA TCCCTCCTTC CTTTAGAAGA GTTCGCTCTG GCAGTGGGAT GTCCGTCATA 
AGCTCTTCGT CTGCAGACCA GCGGCTGCCC GAGGAGCCAT CGTCAGAGGA TGAGCAGCAG 
GTGGAAAAGA AGCTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC 
AACCTGGAGC TGGAGAAGCG CAGGCAGGCC CTCCTGGAGC AGCAGCGCAA GGAGCAGGAG 
CGGCTGGCTC AGCTGGAGCG CGCCGAGCAA GAGAGGAAAG AGCGGGAGCG CCAGGAACAG 
GAGCGCAAGA GACAGCTAGA GCTGGAGAAG CAGCTAGAAA AGCAGCGGGA GCTGGAGAGG 
CAGAGAGAGG AGGAGAGAAG GAAGGAGATC GAGAGGCGGG AGGCAGCAAA ACGGGAGCTC 
GAACGGCAGC GACAGCTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GACTCAGAGG 
AACAAGGACC AGGAGGGCAT CGTGGTTCTG AAGGCGAGGA GGAAGACTCT GGAGTTTGAA 
TTAGAAGCTC TGAATGACAA AAAGCATCAG CTGGAAGGAA AACTTCAGGA TATCAGGTGT 
CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACAAACA AGTCTAGAGA GCTGAGAATT 
GCGGAAATCA CCCACTTACA GCAACAGTTG CAGGAATCTC AGCAGATGCT TGGAAGACTT 
ATTCCAGAGA AGCAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGCTTGCAT 
AGAGATTCAC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAACTGGC CCGGCAGCAG 
CTTCGAGAGC AGCTGGACGA GGTGGAGAAA GAGACCAGGT CAAAGCTGCA GGAGATTGAT 
GTTTTCAACA ACCAGCTGAA GGAACTGAGA GAGATACACA GTAAACAGCA GCTCCAGAAG 
CAGAGGTCCA TCGAGGCGGA GAGGCTAAAG CAGAAAGAGC AGGAGAGGAA GAGCCTGGAG 
TTGGAGAAGC AAAAGGAAGA AGGTCAGAGA CGAGTTCAGG AAAGGGACAA GCAATGGCAG 
GAGCATGTGC AGCAGGAGGA GCAGCAGCGC CCTCGGAAAC CCCACGAGGA GGACAAACTG 
AAAAGGGAAG ACAGTGTCAA GAAGAAGGAG GCGGAAGAGA GAGCCAAGCC GGAAGTGCAA 
GACAAGCAGA GTCGGCTTTT CCATCCACAT CAAGAGCCAG CTAAGCCGGC GCAGGCACCC 
TGGCCCACGA CAGAGAAAGG TCCGCTTACA ATCTCTGCAC AGGAGAGTGC CAAAGTGGTG 
TATTACCGAG CGCTGTACCC CTTTGAGTCC AGAAGTCATG ACGAGATCAC CATCCAGCCA 
GGAGATATAG TCATGGTGGA TGAAAGCCAG ACCGGAGAGC CAGGATGGCT TGGAGGAGAA 
CTGAAGGGGA AGACAGGATG GTTCCCTGCA AACTATGCAG AGAAGATTCC AGAAAATGAG 
ATTCCCACTC CAGCCAAACC AGTGACCGAT CTGACATCTG CCCCTGCCCC CAAACTGGCT 
CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG ACCTCTTCTG AGCCCTCCAC AACCCCCAAC 
AACTGGGCAG ACTTCAGTTC CACGTGGCCC AGCAGCACAA ATGAGAAACC AGAAACGGAC 
AACTGGGACA CGTGGGCGGC TCAGCCTTCT CTGACTGTAC CCAGTGCTGG CCAGTTAAGG 
CAGAGGTCGG CCTTCACACC AGCCACAGCC ACTGGCTCCT CCCCTTCTCC CGTCCTGGGC 
CAGGGTGAAA AGGTGGAAGG GCTCCAAGCA CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA 
GACAACCACT TAAATTTTAA CAAAAGTGAT GTCATCACCG TTCTGGAACA GCAAGACATG 
TGGTGGTTTG GAGAAGTTCA AGGTCAGAAG GGTTGGTTCC CCAAGTCTTA CGTGAAACTC 
ATTTCAGGGC CCGTAAGGAA ATCCACAAGC ATCGATACTG GCCCTACGGA AGCTCCCTCT 
AGTCTAAAGA GAGTGGCTTC CCCAGCCGCC AAGCCAGCCA TTCCTGGAGA AGAATTTGTT 
GCTATGTACA CTTACGAGAG TTCTGAGCAT GGAGATTTAA CCTTTCAGCA AGGGGATGTG 
ATTGTGGTTA CCAAGAAGGA TGGTGACTGG TGGACGGGCA CAGTGGGCGA GACGTCCGGA 
GTCTTCCCTT CTAACTATGT GAGGCTTAAA GACTCAGAGG GCTCTGGAAC TGCGGGGAAA 
ACAGGGAGTT TAGGAAAAAA ACCTGAAATC GCCCAGGTCA TTGCTTCCTA CACTGCTACG 
GGTCCTGAAC AGCTCACCCT GGCTCCTGGT CAGCTGATTT TGATCCGAAA AAAGAACCCC 
GGTGGATGGT GGGAAGGAGA ACTACAAGCT CGAGGGAAAA AGCGCCAGAT AGGGTGGTTC 
CCAGCAAATT ATGTCAAACT TCTAAGCCCT GGAACAAGCA AAATCACCCC AACTGAGCTA 
CCCAAGACCG CAGTGCAGCC AGCAGTGTGC CAGGTGATCG GGATGTACGA TTACACCGCG 
CAGAATGACG ATGAGCTAGC CTTCAGCAAA GGCCAGATCA TCAACGTCCT CAGCAAGGAG 
GACCCGGACT GGTGGAAAGG AGAAGTCAGT GGGCAGGTTG GGCTCTTCCC GTCCAATTAT 
GTGAAGCTGA CCACAGACAT GGACCCCAGC CAGCAATGGT GCTCAGACTT ACATCTCTTA 
GATATGTTGA CCCCGACTGA GAGGAAGAGG CAAGGCTACA TCCATGAACT CATTGTCACA 
GAGGAGAACT ATGTGAACGA CCTGCAGCTG GTCACAGAGA TCTTTCAGAA ACCCCTGACG 
GAGTCTGAGC TGCTGACAGA AAAAGAGGTT GCTATGATTT TTGTTAACTG GAAGGAGCTG 
ATTATGTGTA ATATCAAACT GCTGAAGGCG CTGAGAGTCC GCAAGAAGAT GTCCGGAGAG 
AAGATGCCGG TGAAGATGAT CGGTGACATC CTGAGCGCCC AGCTGCCCCA CATGCAGCCT 
TACATCCGCT TCTGCAGCTG CCAGCTCAAT GGGGCCGCCC TCATCCAGCA GAAGACAGAT 
GAGGCTCCAG ACTTCAAGGA GTTCGTCAAA AGACTGGCAA TGGACCCTCG GTGCAAAGGC 
ATGCCACTGT CCAGCTTCAT ACTGAAACCT ATGCAGCGTG TCACAAGATA CCCACTGATC 
ATTAAAAACA TCCTGGAAAA CACTCCTGAA AACCACCCCG ACCACAGCCA CCTGAAGCAC 
GCCCTGGAGA AAGCAGAAGA GCTGTGCTCC CAAGTGAACG AGGGAGTTCG AGAGAAGGAG 
AACTCTGACC GGCTGGAGTG GATCCAGGCG CATGTGCAGT GCGAAGGCCT TTCTGAGCAA 
CTGGTGTTCA ATTCGGTGAC CAACTGCTTG GGACCACGCA AGTTTCTGCA CAGCGGGAAG 
CTCTACAAGG CCAAGAGCAA CAAAGAGCTG TATGGCTTCC TCTTCAATGA CTTCCTCCTG 
CTGACCCAAA TCACAAAGCC CTTAGGCTCT TCCAGCACTG ACAAAGTCTT CAGCCCCAAA 
TCTAACCTGC AGTATAAAAT GTACAAAACG CCCATTTTCT TAAATGAGGT TCTAGTAAAA 
TTGCCCACGG ACCCTTCTGG AGATGAACCT ATCTTTCACA TTTCGCACAT TGACCGAGTC 
TACACGCTCC GAGCAGAAAG TATAAATGAG AGGACTGCCT GGGTGCAGAA AATCAAGGCC 
GCATCTGAGC TCTACATAGA GACGGAGAAA AAGAAGCGAG AGAAGGCGTA TCTGGTCCGT 
TCCCAGAGAG CGACCGGTAT TGGAAGATTG ATGGTGAACG TAGTAGAAGG CATTGAGCTG 
AAGCCCTGTC GGTCACATGG AAAGAGCAAC CCGTACTGTG AGGTGACCAT GGGCTCTCAG 
TGCCACATCA CCAAGACAAT CCAGGACACG CTGAACCCTA AGTGGAATTC TAACTGCCAG 
TTCTTCATCA GAGACCTGGA ACAGGAGGTT CTCTGCATCA CTGTGTTTGA GAGGGACCAA 
TTCTCGCCAG ACGATTTTTT GGGTCGGACA GAGATCCGAG TGGCCGACAT CAAGAAAGAC 
CAGGGCTCCA AGGGGCCAGT TACAAAGTGT CTCCTGCTGC ATGAGGTGCC CACAGGAGAG 
ATTGTGGTTC GCCTTGACCT GCAGTTGTTT GATGAGCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129568.1
CDS	62..5203
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFQSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNKDGRMDQVEFSIAMKLIKLKLQGYQLPPALPPVMKQQPAAISSAPAFGIGGMAGMPPLTAVAPVPMGSIPVVGMSPPLVSSVPQAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASAPAAAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGMSVISSSSADQRLPEEPSSEDEQQVEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLTQRNKDQEGIVVLKARRKTLEFELEALNDKKHQLEGKLQDIRCRLATQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILSDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLREQLDEVEKETRSKLQEIDVFNNQLKELREIHSKQQLQKQRSIEAERLKQKEQERKSLELEKQKEEGQRRVQERDKQWQEHVQQEEQQRPRKPHEEDKLKREDSVKKKEAEERAKPEVQDKQSRLFHPHQEPAKPAQAPWPTTEKGPLTISAQESAKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEIPTPAKPVTDLTSAPAPKLALRETPAPLPVTSSEPSTTPNNWADFSSTWPSSTNEKPETDNWDTWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKSDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPVRKSTSIDTGPTEAPSSLKRVASPAAKPAIPGEEFVAMYTYESSEHGDLTFQQGDVIVVTKKDGDWWTGTVGETSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTELPKTAVQPAVCQVIGMYDYTAQNDDELAFSKGQIINVLSKEDPDWWKGEVSGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLTESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSSTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	NM_001136096.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus intersectin 1 (Itsn1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136096.1

ORF Insert Sequence:

ATGGCTCAGT TTCCGACACC TTTTGGTGGG AGCCTGGACA TCTGGGCCAT AACCGTGGAG 
GAAAGAGCCA AGCATGACCA GCAGTTCCAG AGTTTGAAGC CGATATCTGG ATTCATCACT 
GGTGATCAAG CAAGGAACTT CTTTTTCCAA TCTGGGTTAC CTCAGCCCGT CTTAGCACAA 
ATATGGGCGC TGGCTGACAT GAATAAGGAC GGAAGGATGG ATCAGGTGGA GTTTTCCATA 
GCCATGAAGC TCATCAAACT GAAGCTGCAA GGATATCAGC TCCCCCCCGC ACTTCCCCCT 
GTCATGAAGC AGCAGCCAGC GGCCATCTCT AGTGCACCAG CGTTTGGTAT AGGAGGGATG 
GCTGGAATGC CACCACTGAC AGCTGTTGCT CCCGTGCCAA TGGGCTCCAT CCCAGTTGTT 
GGAATGTCTC CGCCCTTAGT ATCTTCTGTC CCTCAAGCAG CAGTGCCTCC CCTGGCTAAC 
GGGGCCCCTC CTGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTCAGCAG ATCTGGTCCA GGGTCACAAT TAAACACTAA ACTACAGAAG 
GCACAATCAT TTGATGTAGC CAGCGCCCCT GCAGCGGCAG AATGGGCTGT GCCTCAGTCG 
TCAAGACTGA AATACAGGCA GTTATTCAAC AGTCACGACA AGACCATGAG TGGACACTTA 
ACAGGTCCCC AGGCAAGAAC TATTCTCATG CAATCAAGTT TACCCCAGGC TCAGCTGGCT 
TCAATCTGGA ATCTTTCCGA CATTGATCAA GATGGAAAGC TCACCGCAGA AGAATTCATC 
CTAGCGATGC ACCTGATTGA TGTTGCTATG TCTGGTCAGC CACTGCCGCC TGTCCTGCCT 
CCAGAATACA TCCCTCCTTC CTTTAGAAGA GTTCGCTCTG GCAGTGGGAT GTCCGTCATA 
AGCTCTTCGT CTGCAGACCA GCGGCTGCCC GAGGAGCCAT CGTCAGAGGA TGAGCAGCAG 
GTGGAAAAGA AGCTGCCTGT GACATTTGAA GATAAGAAGC GGGAGAACTT CGAGCGAGGC 
AACCTGGAGC TGGAGAAGCG CAGGCAGGCC CTCCTGGAGC AGCAGCGCAA GGAGCAGGAG 
CGGCTGGCTC AGCTGGAGCG CGCCGAGCAA GAGAGGAAAG AGCGGGAGCG CCAGGAACAG 
GAGCGCAAGA GACAGCTAGA GCTGGAGAAG CAGCTAGAAA AGCAGCGGGA GCTGGAGAGG 
CAGAGAGAGG AGGAGAGAAG GAAGGAGATC GAGAGGCGGG AGGCAGCAAA ACGGGAGCTC 
GAACGGCAGC GACAGCTTGA ATGGGAACGG AACCGGAGAC AGGAACTCCT GACTCAGAGG 
AACAAGGACC AGGAGGGCAT CGTGGTTCTG AAGGCGAGGA GGAAGACTCT GGAGTTTGAA 
TTAGAAGCTC TGAATGACAA AAAGCATCAG CTGGAAGGAA AACTTCAGGA TATCAGGTGT 
CGACTGGCAA CCCAGAGGCA AGAAATTGAG AGCACAAACA AGTCTAGAGA GCTGAGAATT 
GCGGAAATCA CCCACTTACA GCAACAGTTG CAGGAATCTC AGCAGATGCT TGGAAGACTT 
ATTCCAGAGA AGCAGATACT CAGTGACCAG TTAAAACAAG TCCAGCAGAA CAGCTTGCAT 
AGAGATTCAC TTCTTACCCT CAAAAGAGCC TTGGAAGCAA AGGAACTGGC CCGGCAGCAG 
CTTCGAGAGC AGCTGGACGA GGTGGAGAAA GAGACCAGGT CAAAGCTGCA GGAGATTGAT 
GTTTTCAACA ACCAGCTGAA GGAACTGAGA GAGATACACA GTAAACAGCA GCTCCAGAAG 
CAGAGGTCCA TCGAGGCGGA GAGGCTAAAG CAGAAAGAGC AGGAGAGGAA GAGCCTGGAG 
TTGGAGAAGC AAAAGGAAGA AGGTCAGAGA CGAGTTCAGG AAAGGGACAA GCAATGGCAG 
GAGCATGTGC AGCAGGAGGA GCAGCAGCGC CCTCGGAAAC CCCACGAGGA GGACAAACTG 
AAAAGGGAAG ACAGTGTCAA GAAGAAGGAG GCGGAAGAGA GAGCCAAGCC GGAAGTGCAA 
GACAAGCAGA GTCGGCTTTT CCATCCACAT CAAGAGCCAG CTAAGCCGGC GCAGGCACCC 
TGGCCCACGA CAGAGAAAGG TCCGCTTACA ATCTCTGCAC AGGAGAGTGC CAAAGTGGTG 
TATTACCGAG CGCTGTACCC CTTTGAGTCC AGAAGTCATG ACGAGATCAC CATCCAGCCA 
GGAGATATAG TCATGGTGGA TGAAAGCCAG ACCGGAGAGC CAGGATGGCT TGGAGGAGAA 
CTGAAGGGGA AGACAGGATG GTTCCCTGCA AACTATGCAG AGAAGATTCC AGAAAATGAG 
ATTCCCACTC CAGCCAAACC AGTGACCGAT CTGACATCTG CCCCTGCCCC CAAACTGGCT 
CTGCGTGAGA CCCCTGCTCC TTTGCCAGTG ACCTCTTCTG AGCCCTCCAC AACCCCCAAC 
AACTGGGCAG ACTTCAGTTC CACGTGGCCC AGCAGCACAA ATGAGAAACC AGAAACGGAC 
AACTGGGACA CGTGGGCGGC TCAGCCTTCT CTGACTGTAC CCAGTGCTGG CCAGTTAAGG 
CAGAGGTCGG CCTTCACACC AGCCACAGCC ACTGGCTCCT CCCCTTCTCC CGTCCTGGGC 
CAGGGTGAAA AGGTGGAAGG GCTCCAAGCA CAAGCCCTGT ATCCCTGGAG AGCCAAAAAA 
GACAACCACT TAAATTTTAA CAAAAGTGAT GTCATCACCG TTCTGGAACA GCAAGACATG 
TGGTGGTTTG GAGAAGTTCA AGGTCAGAAG GGTTGGTTCC CCAAGTCTTA CGTGAAACTC 
ATTTCAGGGC CCGTAAGGAA ATCCACAAGC ATCGATACTG GCCCTACGGA AGCTCCCTCT 
AGTCTAAAGA GAGTGGCTTC CCCAGCCGCC AAGCCAGCCA TTCCTGGAGA AGAATTTGTT 
GCTATGTACA CTTACGAGAG TTCTGAGCAT GGAGATTTAA CCTTTCAGCA AGGGGATGTG 
ATTGTGGTTA CCAAGAAGGA TGGTGACTGG TGGACGGGCA CAGTGGGCGA GACGTCCGGA 
GTCTTCCCTT CTAACTATGT GAGGCTTAAA GACTCAGAGG GCTCTGGAAC TGCGGGGAAA 
ACAGGGAGTT TAGGAAAAAA ACCTGAAATC GCCCAGGTCA TTGCTTCCTA CACTGCTACG 
GGTCCTGAAC AGCTCACCCT GGCTCCTGGT CAGCTGATTT TGATCCGAAA AAAGAACCCC 
GGTGGATGGT GGGAAGGAGA ACTACAAGCT CGAGGGAAAA AGCGCCAGAT AGGGTGGTTC 
CCAGCAAATT ATGTCAAACT TCTAAGCCCT GGAACAAGCA AAATCACCCC AACTGAGCTA 
CCCAAGACCG CAGTGCAGCC AGCAGTGTGC CAGGTGATCG GGATGTACGA TTACACCGCG 
CAGAATGACG ATGAGCTAGC CTTCAGCAAA GGCCAGATCA TCAACGTCCT CAGCAAGGAG 
GACCCGGACT GGTGGAAAGG AGAAGTCAGT GGGCAGGTTG GGCTCTTCCC GTCCAATTAT 
GTGAAGCTGA CCACAGACAT GGACCCCAGC CAGCAATGGT GCTCAGACTT ACATCTCTTA 
GATATGTTGA CCCCGACTGA GAGGAAGAGG CAAGGCTACA TCCATGAACT CATTGTCACA 
GAGGAGAACT ATGTGAACGA CCTGCAGCTG GTCACAGAGA TCTTTCAGAA ACCCCTGACG 
GAGTCTGAGC TGCTGACAGA AAAAGAGGTT GCTATGATTT TTGTTAACTG GAAGGAGCTG 
ATTATGTGTA ATATCAAACT GCTGAAGGCG CTGAGAGTCC GCAAGAAGAT GTCCGGAGAG 
AAGATGCCGG TGAAGATGAT CGGTGACATC CTGAGCGCCC AGCTGCCCCA CATGCAGCCT 
TACATCCGCT TCTGCAGCTG CCAGCTCAAT GGGGCCGCCC TCATCCAGCA GAAGACAGAT 
GAGGCTCCAG ACTTCAAGGA GTTCGTCAAA AGACTGGCAA TGGACCCTCG GTGCAAAGGC 
ATGCCACTGT CCAGCTTCAT ACTGAAACCT ATGCAGCGTG TCACAAGATA CCCACTGATC 
ATTAAAAACA TCCTGGAAAA CACTCCTGAA AACCACCCCG ACCACAGCCA CCTGAAGCAC 
GCCCTGGAGA AAGCAGAAGA GCTGTGCTCC CAAGTGAACG AGGGAGTTCG AGAGAAGGAG 
AACTCTGACC GGCTGGAGTG GATCCAGGCG CATGTGCAGT GCGAAGGCCT TTCTGAGCAA 
CTGGTGTTCA ATTCGGTGAC CAACTGCTTG GGACCACGCA AGTTTCTGCA CAGCGGGAAG 
CTCTACAAGG CCAAGAGCAA CAAAGAGCTG TATGGCTTCC TCTTCAATGA CTTCCTCCTG 
CTGACCCAAA TCACAAAGCC CTTAGGCTCT TCCAGCACTG ACAAAGTCTT CAGCCCCAAA 
TCTAACCTGC AGTATAAAAT GTACAAAACG CCCATTTTCT TAAATGAGGT TCTAGTAAAA 
TTGCCCACGG ACCCTTCTGG AGATGAACCT ATCTTTCACA TTTCGCACAT TGACCGAGTC 
TACACGCTCC GAGCAGAAAG TATAAATGAG AGGACTGCCT GGGTGCAGAA AATCAAGGCC 
GCATCTGAGC TCTACATAGA GACGGAGAAA AAGAAGCGAG AGAAGGCGTA TCTGGTCCGT 
TCCCAGAGAG CGACCGGTAT TGGAAGATTG ATGGTGAACG TAGTAGAAGG CATTGAGCTG 
AAGCCCTGTC GGTCACATGG AAAGAGCAAC CCGTACTGTG AGGTGACCAT GGGCTCTCAG 
TGCCACATCA CCAAGACAAT CCAGGACACG CTGAACCCTA AGTGGAATTC TAACTGCCAG 
TTCTTCATCA GAGACCTGGA ACAGGAGGTT CTCTGCATCA CTGTGTTTGA GAGGGACCAA 
TTCTCGCCAG ACGATTTTTT GGGTCGGACA GAGATCCGAG TGGCCGACAT CAAGAAAGAC 
CAGGGCTCCA AGGGGCCAGT TACAAAGTGT CTCCTGCTGC ATGAGGTGCC CACAGGAGAG 
ATTGTGGTTC GCCTTGACCT GCAGTTGTTT GATGAGCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Altered Expression of Intersectin1-L in Patients with Refractory Epilepsy and in Experimental Epileptic Rats.
Cellular and molecular neurobiology35(6)871-80(2015 Aug)
Yang X,Xu X,Zhang Y,Wang S,Li M,Wang X

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Science (New York, N.Y.)344(6187)1023-8(2014 May)
Wilhelm BG,Mandad S,Truckenbrodt S,Kr?hnert K,Sch?fer C,Rammner B,Koo SJ,Cla?en GA,Krauss M,Haucke V,Urlaub H,Rizzoli SO

Fast neurotransmitter release regulated by the endocytic scaffold intersectin.
Proceedings of the National Academy of Sciences of the United States of America110(20)8266-71(2013 May)
Sakaba T,Kononenko NL,Bacetic J,Pechstein A,Schmoranzer J,Yao L,Barth H,Shupliakov O,Kobler O,Aktories K,Haucke V

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Itsn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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