itsn1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004912127.3	XP_004912184.1	intersectin-1 isoform X2
XM_004912128.3	XP_004912185.1	intersectin-1 isoform X3
XM_002939344.4	XP_002939390.1	intersectin-1 isoform X1
XM_002939344.5	XP_002939390.1	intersectin-1 isoform X1
XM_004912129.3	XP_004912186.1	intersectin-1 isoform X4
XM_004912128.4	XP_004912185.1	intersectin-1 isoform X3
XM_004912130.3	XP_004912187.1	intersectin-1 isoform X5
XM_004912129.4	XP_004912186.1	intersectin-1 isoform X4
XM_004912130.4	XP_004912187.1	intersectin-1 isoform X5
XM_004912127.4	XP_004912184.1	intersectin-1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa06418	XM_004912130.4 Latest version!	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa06414	XM_002939344.5 Latest version!	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa06415	XM_004912127.4 Latest version!	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa06416	XM_004912128.4 Latest version!	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa06417	XM_004912129.4 Latest version!	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OXa06414	XM_002939344.4	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa06416	XM_004912128.3	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa06417	XM_004912129.3	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa06418	XM_004912130.3	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa06415	XM_004912127.3	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

itsn1 ( XM_004912130.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912187.1 Xenopus tropicalis intersectin 1 (itsn1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa06418
Clone ID Related Accession (Same CDS sequence)	XM_004912130.3 , XM_004912130.4
Accession Version	XM_004912130.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intersectin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912130.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGGGATGA GTGGAATCAG TGGCATTCCT CCCCTAGCAG CTGTAGCCCC TGTGCCAATG 
CCATCCATCC CAGTAGTTGG AATGTCTCCT CCTCTTGTGT CCTCTGTCCC CACAGTGCCA 
CCCTTGGCTA ACGGAGCTCC TGCAGTAATA CAGCCTCTTC CTGCTTTCGC TCACTCTGCC 
ACGTTGCCAA AGAGTTCTTC ATTCGGCCGC TCAGGTGCCG GCTCCCAGAT GAACACTAAA 
CTTCAGAAAG CTCAGTCCTT TGATGTCCCA ACCCCCCCAC CCCTGGCAGA ATGGGCTGTA 
CCACAGTCTT CAAGGTTGAA GTACAGACAG TTGTTCAACA GCCACGACAA AACCATGAGT 
GGGCATTTAA CAGGTCCTCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCGCAGAGC 
CAGCTAGCTA CGATATGGAA CCTTTCAGAC ATTGACCTAG ATGGAAAACT AACGGCCGAA 
GAATTTATTT TAGCTATGCA CTTAATAGAT GTGGCCATGT CTGGCCAGCC ACTTCCTCCA 
GTTCTGCCTC CTGAGTATAT TCCCCCATCT TTTAGAAGAG TTCGATCGGG GAGCGGGTTA 
TCGATTATGA GCTCGGTGTC TGTAGATCAG CGCTTACCGG AGGAACCAGC AGAAGAGGAG 
CCACAGAACA CAGACAAAAG ACTTCCAGTG ACATTTGAAG ACAAGAAAAG GGAAAACTTT 
GAGCGAGGAA ACCTGGAATT AGAAAAGAGG AGACAGGCAC TTCTAGAGCA GCAGCGCAAA 
GAGCAGGAGA GGCTGGCACA GCTGGAAAGG GCAGAGCAGG AGAGAAAAGA ACGAGAGCGG 
CAAGATCAAG AGCGCAAGCG GCAGCAAGAG CTGGAAAAAC AGCTGGAGAA ACAACGAGAG 
CTGGAGCGGC AACGGGAAGA GGAGCGCAGG AAAGAAATAG AAAGGCGAGA GGCAGCAAAG 
AGAGAACTTG AAAGGCAGAG GCAGTTGGAG TGGGAACGAA ACCGGAGGCA AGAACTCCTT 
AATCAAAGGA ACAGAGAGCA AGAGGACATT GTTGTCTTAA AAGCAAAGAA AAAGACTCTT 
GAGTTTGAGC TGGAAGCTCT AAATGATAAA AAGCACCAGC TGGAAGGGAA ACTTCAGGAT 
ATCCGCTGTC GCCTCACTAC TCAACGGCAC GAAATAGAAA GCACTAACAA ATCCAGGGAG 
CTTAGGATCG CAGAGATCAC GCATTTACAG CAGCAGCTTC AGGAATCTCA GCAGCTGCTT 
GGAAAACTGA TTCCTGAGAA ACAGTCCCTT AACGATCAAT TAAAGCAGGT TCAACAAAAC 
AGTTTGCACA GAGATTCCTT GCTGACACTA AAAAGGGCAT TGGAAGCCAA GGAGATGGGC 
CGCCAGCAGC TCCGAGACCA GTTGGATGAA GTAGAAAAAG AAACACGAGC TAAGCTCCAG 
GAAATCGATG TGTTTAATAA CCAATTAAAG GAACTGAGAG AATTGTACAA CAGACAACAG 
CTCCAGAAAC AGAAGGACTT TGAAGCCGAG AAGATCAAGC AGAAAGAGCT GGAACGGAAA 
ACCAGTGAGC TGGACAAGAT GAAAGAGGAG GACAAAAGGC GGATGCTAGA GCAGGATAAG 
CTTTGGCAGG ATCGTGTGAA GCAGGAAGAG GAACGTTACA AATTCCAAGA TGAGGAGAAG 
CAGAAAAGGG AAGAATCCGC TCAACAATGT GAGGAAGAAA AGAAACCAGA GATCCAAGAG 
AAGCCCAACA AACTGTTCCA CCTACCACCG GAGCCAGGAA AGCTAGGAGG GCATGTCCCC 
TGGATGAATA CAGAGAAAGC ACCGCTAACA TTCAACCAGG GTGATGTTAA GGTTGTGTAT 
TACCGGGCAC TCTACCCTTT TGATGCTCGA AGTCATGATG AAATCACTAT TCAGCCTGGG 
GACATTATTA TGGTGGATGA GAGCCAAACC GGAGAGCCTG GATGGCTGGG AGGTGAACTG 
AAGGGAAAAA CTGGCTGGTT TCCTGCCAAC TATGCAGAGA GAATGCCCGA GGGTGAATTC 
CCAAGCACAA CTAAACAGGC AGCAGACACT ACGGCCAAAC CCACTGTCCA TTTGGCACCT 
TCCACTCCCA CACCTGCAGC TTTTACAGAT AACTCTGCAA ATTCCAACAA CTGGGCAGAC 
TTCAGCTCCA CGTGGCCAAC CAATAACACT GATAAAGCAG AGACGGATAA CTGGGATACA 
TGGGGAGCTC AACCATCTCT GACTGTTCCT AGTGCCGGAC AGCACAGGCA GCGCTCTGCT 
TTTACCCCAG CAACGGTTAC TGGATCATCT CCATCTCCTG TACTTGGTCA GGGTGAAAAA 
GTAGAAGGGC TTCAGGCACA AGCCTTGTAT CCTTGGAGAG CAAAAAAGGA CAACCACCTT 
AATTTTAACA AAAATGACAT CATCACGGTT CTAGAGCAGC AGGATATGTG GTGGTTTGGG 
GAAGTTCAAG GTCAAAAGGG CTGGTTTCCC AAATCCTACG TAAAACTTAT ATCCGGGCCA 
CTAAGGAAAT CTACAAGCAT CGATTCCACA TCTTCAGAAA GCCCTGCTAG TTTAAAAAGA 
GTCTCTTCAC CAGCATTTAA ACCTGCCATG CAAGGAGAAG AATACATAGC CATGTACACG 
TACGAGAGCA ACGAGCAGGG AGACTTGACC TTTCAGCAAG GAGATCTGAT TGTGGTGATT 
AAAAAAGATG GGGACTGGTG GACTGGCACG GTGGGAGAGA AGACGGGGGT TTTCCCTTCC 
AACTACGTAA GGCCCAAAGA CTCCGAGGCG GCTGGAAGCG CAGGAAAGAC AGGAAGCCTT 
GGGAAGAAAC CAGAAATTGC CCAGGTTATT GCTTCTTACA CAGCAACTGG CCCTGAGCAG 
CTGACACTGG CTCCTGGCCA ACTTATCCTC ATCCGGAAAA AGAATCCTGG AGGATGGTGG 
GAAGGAGAAC TGCAGGCACG TGGGAAGAAG CGACAGATTG GATGGTTTCC AGCAAATTAT 
GTGAAGCTTT TGAGCCCTGG TACAAACAAA AGCACGCCGA CAGAACCACC AAAAGCAACA 
TCATTGCCTC CAACATGCCA AGTCATCGGG ATGTACGATT ATATCGCTCA GAATGACGAT 
GAGTTGGCCT TCGGTAAAGG TCAGGTCATT AACGTGCTGA ACAAAGAGGA CCCGGACTGG 
TGGAAAGGAG AACTGAACGG CCATGTTGGT CTGTTTCCCT CCAACTATGT GAAACTGACC 
ACAGACATGG ATCCCAGTCA GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912187.1
CDS	258..3884
Translation	MAQFGTPFGGNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSAAAAAAAAAGFGMSGISGIPPLAAVAPVPMPSIPVVGMSPPLVSSVPTVPPLANGAPAVIQPLPAFAHSATLPKSSSFGRSGAGSQMNTKLQKAQSFDVPTPPPLAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGLSIMSSVSVDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRELRIAEITHLQQQLQESQQLLGKLIPEKQSLNDQLKQVQQNSLHRDSLLTLKRALEAKEMGRQQLRDQLDEVEKETRAKLQEIDVFNNQLKELRELYNRQQLQKQKDFEAEKIKQKELERKTSELDKMKEEDKRRMLEQDKLWQDRVKQEEERYKFQDEEKQKREESAQQCEEEKKPEIQEKPNKLFHLPPEPGKLGGHVPWMNTEKAPLTFNQGDVKVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEPGWLGGELKGKTGWFPANYAERMPEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTWPTNNTDKAETDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVTGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRVSSPAFKPAMQGEEYIAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTNKSTPTEPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGELNGHVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	XM_004912130.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912130.4

ORF Insert Sequence:

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGGGATGA GTGGAATCAG TGGCATTCCT CCCCTAGCAG CTGTAGCCCC TGTGCCAATG 
CCATCCATCC CAGTAGTTGG AATGTCTCCT CCTCTTGTGT CCTCTGTCCC CACAGTGCCA 
CCCTTGGCTA ACGGAGCTCC TGCAGTAATA CAGCCTCTTC CTGCTTTCGC TCACTCTGCC 
ACGTTGCCAA AGAGTTCTTC ATTCGGCCGC TCAGGTGCCG GCTCCCAGAT GAACACTAAA 
CTTCAGAAAG CTCAGTCCTT TGATGTCCCA ACCCCCCCAC CCCTGGCAGA ATGGGCTGTA 
CCACAGTCTT CAAGGTTGAA GTACAGACAG TTGTTCAACA GCCACGACAA AACCATGAGT 
GGGCATTTAA CAGGTCCTCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCGCAGAGC 
CAGCTAGCTA CGATATGGAA CCTTTCAGAC ATTGACCTAG ATGGAAAACT AACGGCCGAA 
GAATTTATTT TAGCTATGCA CTTAATAGAT GTGGCCATGT CTGGCCAGCC ACTTCCTCCA 
GTTCTGCCTC CTGAGTATAT TCCCCCATCT TTTAGAAGAG TTCGATCGGG GAGCGGGTTA 
TCGATTATGA GCTCGGTGTC TGTAGATCAG CGCTTACCGG AGGAACCAGC AGAAGAGGAG 
CCACAGAACA CAGACAAAAG ACTTCCAGTG ACATTTGAAG ACAAGAAAAG GGAAAACTTT 
GAGCGAGGAA ACCTGGAATT AGAAAAGAGG AGACAGGCAC TTCTAGAGCA GCAGCGCAAA 
GAGCAGGAGA GGCTGGCACA GCTGGAAAGG GCAGAGCAGG AGAGAAAAGA ACGAGAGCGG 
CAAGATCAAG AGCGCAAGCG GCAGCAAGAG CTGGAAAAAC AGCTGGAGAA ACAACGAGAG 
CTGGAGCGGC AACGGGAAGA GGAGCGCAGG AAAGAAATAG AAAGGCGAGA GGCAGCAAAG 
AGAGAACTTG AAAGGCAGAG GCAGTTGGAG TGGGAACGAA ACCGGAGGCA AGAACTCCTT 
AATCAAAGGA ACAGAGAGCA AGAGGACATT GTTGTCTTAA AAGCAAAGAA AAAGACTCTT 
GAGTTTGAGC TGGAAGCTCT AAATGATAAA AAGCACCAGC TGGAAGGGAA ACTTCAGGAT 
ATCCGCTGTC GCCTCACTAC TCAACGGCAC GAAATAGAAA GCACTAACAA ATCCAGGGAG 
CTTAGGATCG CAGAGATCAC GCATTTACAG CAGCAGCTTC AGGAATCTCA GCAGCTGCTT 
GGAAAACTGA TTCCTGAGAA ACAGTCCCTT AACGATCAAT TAAAGCAGGT TCAACAAAAC 
AGTTTGCACA GAGATTCCTT GCTGACACTA AAAAGGGCAT TGGAAGCCAA GGAGATGGGC 
CGCCAGCAGC TCCGAGACCA GTTGGATGAA GTAGAAAAAG AAACACGAGC TAAGCTCCAG 
GAAATCGATG TGTTTAATAA CCAATTAAAG GAACTGAGAG AATTGTACAA CAGACAACAG 
CTCCAGAAAC AGAAGGACTT TGAAGCCGAG AAGATCAAGC AGAAAGAGCT GGAACGGAAA 
ACCAGTGAGC TGGACAAGAT GAAAGAGGAG GACAAAAGGC GGATGCTAGA GCAGGATAAG 
CTTTGGCAGG ATCGTGTGAA GCAGGAAGAG GAACGTTACA AATTCCAAGA TGAGGAGAAG 
CAGAAAAGGG AAGAATCCGC TCAACAATGT GAGGAAGAAA AGAAACCAGA GATCCAAGAG 
AAGCCCAACA AACTGTTCCA CCTACCACCG GAGCCAGGAA AGCTAGGAGG GCATGTCCCC 
TGGATGAATA CAGAGAAAGC ACCGCTAACA TTCAACCAGG GTGATGTTAA GGTTGTGTAT 
TACCGGGCAC TCTACCCTTT TGATGCTCGA AGTCATGATG AAATCACTAT TCAGCCTGGG 
GACATTATTA TGGTGGATGA GAGCCAAACC GGAGAGCCTG GATGGCTGGG AGGTGAACTG 
AAGGGAAAAA CTGGCTGGTT TCCTGCCAAC TATGCAGAGA GAATGCCCGA GGGTGAATTC 
CCAAGCACAA CTAAACAGGC AGCAGACACT ACGGCCAAAC CCACTGTCCA TTTGGCACCT 
TCCACTCCCA CACCTGCAGC TTTTACAGAT AACTCTGCAA ATTCCAACAA CTGGGCAGAC 
TTCAGCTCCA CGTGGCCAAC CAATAACACT GATAAAGCAG AGACGGATAA CTGGGATACA 
TGGGGAGCTC AACCATCTCT GACTGTTCCT AGTGCCGGAC AGCACAGGCA GCGCTCTGCT 
TTTACCCCAG CAACGGTTAC TGGATCATCT CCATCTCCTG TACTTGGTCA GGGTGAAAAA 
GTAGAAGGGC TTCAGGCACA AGCCTTGTAT CCTTGGAGAG CAAAAAAGGA CAACCACCTT 
AATTTTAACA AAAATGACAT CATCACGGTT CTAGAGCAGC AGGATATGTG GTGGTTTGGG 
GAAGTTCAAG GTCAAAAGGG CTGGTTTCCC AAATCCTACG TAAAACTTAT ATCCGGGCCA 
CTAAGGAAAT CTACAAGCAT CGATTCCACA TCTTCAGAAA GCCCTGCTAG TTTAAAAAGA 
GTCTCTTCAC CAGCATTTAA ACCTGCCATG CAAGGAGAAG AATACATAGC CATGTACACG 
TACGAGAGCA ACGAGCAGGG AGACTTGACC TTTCAGCAAG GAGATCTGAT TGTGGTGATT 
AAAAAAGATG GGGACTGGTG GACTGGCACG GTGGGAGAGA AGACGGGGGT TTTCCCTTCC 
AACTACGTAA GGCCCAAAGA CTCCGAGGCG GCTGGAAGCG CAGGAAAGAC AGGAAGCCTT 
GGGAAGAAAC CAGAAATTGC CCAGGTTATT GCTTCTTACA CAGCAACTGG CCCTGAGCAG 
CTGACACTGG CTCCTGGCCA ACTTATCCTC ATCCGGAAAA AGAATCCTGG AGGATGGTGG 
GAAGGAGAAC TGCAGGCACG TGGGAAGAAG CGACAGATTG GATGGTTTCC AGCAAATTAT 
GTGAAGCTTT TGAGCCCTGG TACAAACAAA AGCACGCCGA CAGAACCACC AAAAGCAACA 
TCATTGCCTC CAACATGCCA AGTCATCGGG ATGTACGATT ATATCGCTCA GAATGACGAT 
GAGTTGGCCT TCGGTAAAGG TCAGGTCATT AACGTGCTGA ACAAAGAGGA CCCGGACTGG 
TGGAAAGGAG AACTGAACGG CCATGTTGGT CTGTTTCCCT CCAACTATGT GAAACTGACC 
ACAGACATGG ATCCCAGTCA GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_004912130.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912187.1 Xenopus tropicalis intersectin 1 (itsn1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa06418
Clone ID Related Accession (Same CDS sequence)	XM_004912130.3 , XM_004912130.4
Accession Version	XM_004912130.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intersectin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912130.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGGGATGA GTGGAATCAG TGGCATTCCT CCCCTAGCAG CTGTAGCCCC TGTGCCAATG 
CCATCCATCC CAGTAGTTGG AATGTCTCCT CCTCTTGTGT CCTCTGTCCC CACAGTGCCA 
CCCTTGGCTA ACGGAGCTCC TGCAGTAATA CAGCCTCTTC CTGCTTTCGC TCACTCTGCC 
ACGTTGCCAA AGAGTTCTTC ATTCGGCCGC TCAGGTGCCG GCTCCCAGAT GAACACTAAA 
CTTCAGAAAG CTCAGTCCTT TGATGTCCCA ACCCCCCCAC CCCTGGCAGA ATGGGCTGTA 
CCACAGTCTT CAAGGTTGAA GTACAGACAG TTGTTCAACA GCCACGACAA AACCATGAGT 
GGGCATTTAA CAGGTCCTCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCGCAGAGC 
CAGCTAGCTA CGATATGGAA CCTTTCAGAC ATTGACCTAG ATGGAAAACT AACGGCCGAA 
GAATTTATTT TAGCTATGCA CTTAATAGAT GTGGCCATGT CTGGCCAGCC ACTTCCTCCA 
GTTCTGCCTC CTGAGTATAT TCCCCCATCT TTTAGAAGAG TTCGATCGGG GAGCGGGTTA 
TCGATTATGA GCTCGGTGTC TGTAGATCAG CGCTTACCGG AGGAACCAGC AGAAGAGGAG 
CCACAGAACA CAGACAAAAG ACTTCCAGTG ACATTTGAAG ACAAGAAAAG GGAAAACTTT 
GAGCGAGGAA ACCTGGAATT AGAAAAGAGG AGACAGGCAC TTCTAGAGCA GCAGCGCAAA 
GAGCAGGAGA GGCTGGCACA GCTGGAAAGG GCAGAGCAGG AGAGAAAAGA ACGAGAGCGG 
CAAGATCAAG AGCGCAAGCG GCAGCAAGAG CTGGAAAAAC AGCTGGAGAA ACAACGAGAG 
CTGGAGCGGC AACGGGAAGA GGAGCGCAGG AAAGAAATAG AAAGGCGAGA GGCAGCAAAG 
AGAGAACTTG AAAGGCAGAG GCAGTTGGAG TGGGAACGAA ACCGGAGGCA AGAACTCCTT 
AATCAAAGGA ACAGAGAGCA AGAGGACATT GTTGTCTTAA AAGCAAAGAA AAAGACTCTT 
GAGTTTGAGC TGGAAGCTCT AAATGATAAA AAGCACCAGC TGGAAGGGAA ACTTCAGGAT 
ATCCGCTGTC GCCTCACTAC TCAACGGCAC GAAATAGAAA GCACTAACAA ATCCAGGGAG 
CTTAGGATCG CAGAGATCAC GCATTTACAG CAGCAGCTTC AGGAATCTCA GCAGCTGCTT 
GGAAAACTGA TTCCTGAGAA ACAGTCCCTT AACGATCAAT TAAAGCAGGT TCAACAAAAC 
AGTTTGCACA GAGATTCCTT GCTGACACTA AAAAGGGCAT TGGAAGCCAA GGAGATGGGC 
CGCCAGCAGC TCCGAGACCA GTTGGATGAA GTAGAAAAAG AAACACGAGC TAAGCTCCAG 
GAAATCGATG TGTTTAATAA CCAATTAAAG GAACTGAGAG AATTGTACAA CAGACAACAG 
CTCCAGAAAC AGAAGGACTT TGAAGCCGAG AAGATCAAGC AGAAAGAGCT GGAACGGAAA 
ACCAGTGAGC TGGACAAGAT GAAAGAGGAG GACAAAAGGC GGATGCTAGA GCAGGATAAG 
CTTTGGCAGG ATCGTGTGAA GCAGGAAGAG GAACGTTACA AATTCCAAGA TGAGGAGAAG 
CAGAAAAGGG AAGAATCCGC TCAACAATGT GAGGAAGAAA AGAAACCAGA GATCCAAGAG 
AAGCCCAACA AACTGTTCCA CCTACCACCG GAGCCAGGAA AGCTAGGAGG GCATGTCCCC 
TGGATGAATA CAGAGAAAGC ACCGCTAACA TTCAACCAGG GTGATGTTAA GGTTGTGTAT 
TACCGGGCAC TCTACCCTTT TGATGCTCGA AGTCATGATG AAATCACTAT TCAGCCTGGG 
GACATTATTA TGGTGGATGA GAGCCAAACC GGAGAGCCTG GATGGCTGGG AGGTGAACTG 
AAGGGAAAAA CTGGCTGGTT TCCTGCCAAC TATGCAGAGA GAATGCCCGA GGGTGAATTC 
CCAAGCACAA CTAAACAGGC AGCAGACACT ACGGCCAAAC CCACTGTCCA TTTGGCACCT 
TCCACTCCCA CACCTGCAGC TTTTACAGAT AACTCTGCAA ATTCCAACAA CTGGGCAGAC 
TTCAGCTCCA CGTGGCCAAC CAATAACACT GATAAAGCAG AGACGGATAA CTGGGATACA 
TGGGGAGCTC AACCATCTCT GACTGTTCCT AGTGCCGGAC AGCACAGGCA GCGCTCTGCT 
TTTACCCCAG CAACGGTTAC TGGATCATCT CCATCTCCTG TACTTGGTCA GGGTGAAAAA 
GTAGAAGGGC TTCAGGCACA AGCCTTGTAT CCTTGGAGAG CAAAAAAGGA CAACCACCTT 
AATTTTAACA AAAATGACAT CATCACGGTT CTAGAGCAGC AGGATATGTG GTGGTTTGGG 
GAAGTTCAAG GTCAAAAGGG CTGGTTTCCC AAATCCTACG TAAAACTTAT ATCCGGGCCA 
CTAAGGAAAT CTACAAGCAT CGATTCCACA TCTTCAGAAA GCCCTGCTAG TTTAAAAAGA 
GTCTCTTCAC CAGCATTTAA ACCTGCCATG CAAGGAGAAG AATACATAGC CATGTACACG 
TACGAGAGCA ACGAGCAGGG AGACTTGACC TTTCAGCAAG GAGATCTGAT TGTGGTGATT 
AAAAAAGATG GGGACTGGTG GACTGGCACG GTGGGAGAGA AGACGGGGGT TTTCCCTTCC 
AACTACGTAA GGCCCAAAGA CTCCGAGGCG GCTGGAAGCG CAGGAAAGAC AGGAAGCCTT 
GGGAAGAAAC CAGAAATTGC CCAGGTTATT GCTTCTTACA CAGCAACTGG CCCTGAGCAG 
CTGACACTGG CTCCTGGCCA ACTTATCCTC ATCCGGAAAA AGAATCCTGG AGGATGGTGG 
GAAGGAGAAC TGCAGGCACG TGGGAAGAAG CGACAGATTG GATGGTTTCC AGCAAATTAT 
GTGAAGCTTT TGAGCCCTGG TACAAACAAA AGCACGCCGA CAGAACCACC AAAAGCAACA 
TCATTGCCTC CAACATGCCA AGTCATCGGG ATGTACGATT ATATCGCTCA GAATGACGAT 
GAGTTGGCCT TCGGTAAAGG TCAGGTCATT AACGTGCTGA ACAAAGAGGA CCCGGACTGG 
TGGAAAGGAG AACTGAACGG CCATGTTGGT CTGTTTCCCT CCAACTATGT GAAACTGACC 
ACAGACATGG ATCCCAGTCA GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912187.1
CDS	296..3922
Translation	MAQFGTPFGGNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSAAAAAAAAAGFGMSGISGIPPLAAVAPVPMPSIPVVGMSPPLVSSVPTVPPLANGAPAVIQPLPAFAHSATLPKSSSFGRSGAGSQMNTKLQKAQSFDVPTPPPLAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGLSIMSSVSVDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRELRIAEITHLQQQLQESQQLLGKLIPEKQSLNDQLKQVQQNSLHRDSLLTLKRALEAKEMGRQQLRDQLDEVEKETRAKLQEIDVFNNQLKELRELYNRQQLQKQKDFEAEKIKQKELERKTSELDKMKEEDKRRMLEQDKLWQDRVKQEEERYKFQDEEKQKREESAQQCEEEKKPEIQEKPNKLFHLPPEPGKLGGHVPWMNTEKAPLTFNQGDVKVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEPGWLGGELKGKTGWFPANYAERMPEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTWPTNNTDKAETDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVTGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRVSSPAFKPAMQGEEYIAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTNKSTPTEPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGELNGHVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	XM_004912130.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912130.3

ORF Insert Sequence:

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGGGATGA GTGGAATCAG TGGCATTCCT CCCCTAGCAG CTGTAGCCCC TGTGCCAATG 
CCATCCATCC CAGTAGTTGG AATGTCTCCT CCTCTTGTGT CCTCTGTCCC CACAGTGCCA 
CCCTTGGCTA ACGGAGCTCC TGCAGTAATA CAGCCTCTTC CTGCTTTCGC TCACTCTGCC 
ACGTTGCCAA AGAGTTCTTC ATTCGGCCGC TCAGGTGCCG GCTCCCAGAT GAACACTAAA 
CTTCAGAAAG CTCAGTCCTT TGATGTCCCA ACCCCCCCAC CCCTGGCAGA ATGGGCTGTA 
CCACAGTCTT CAAGGTTGAA GTACAGACAG TTGTTCAACA GCCACGACAA AACCATGAGT 
GGGCATTTAA CAGGTCCTCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCGCAGAGC 
CAGCTAGCTA CGATATGGAA CCTTTCAGAC ATTGACCTAG ATGGAAAACT AACGGCCGAA 
GAATTTATTT TAGCTATGCA CTTAATAGAT GTGGCCATGT CTGGCCAGCC ACTTCCTCCA 
GTTCTGCCTC CTGAGTATAT TCCCCCATCT TTTAGAAGAG TTCGATCGGG GAGCGGGTTA 
TCGATTATGA GCTCGGTGTC TGTAGATCAG CGCTTACCGG AGGAACCAGC AGAAGAGGAG 
CCACAGAACA CAGACAAAAG ACTTCCAGTG ACATTTGAAG ACAAGAAAAG GGAAAACTTT 
GAGCGAGGAA ACCTGGAATT AGAAAAGAGG AGACAGGCAC TTCTAGAGCA GCAGCGCAAA 
GAGCAGGAGA GGCTGGCACA GCTGGAAAGG GCAGAGCAGG AGAGAAAAGA ACGAGAGCGG 
CAAGATCAAG AGCGCAAGCG GCAGCAAGAG CTGGAAAAAC AGCTGGAGAA ACAACGAGAG 
CTGGAGCGGC AACGGGAAGA GGAGCGCAGG AAAGAAATAG AAAGGCGAGA GGCAGCAAAG 
AGAGAACTTG AAAGGCAGAG GCAGTTGGAG TGGGAACGAA ACCGGAGGCA AGAACTCCTT 
AATCAAAGGA ACAGAGAGCA AGAGGACATT GTTGTCTTAA AAGCAAAGAA AAAGACTCTT 
GAGTTTGAGC TGGAAGCTCT AAATGATAAA AAGCACCAGC TGGAAGGGAA ACTTCAGGAT 
ATCCGCTGTC GCCTCACTAC TCAACGGCAC GAAATAGAAA GCACTAACAA ATCCAGGGAG 
CTTAGGATCG CAGAGATCAC GCATTTACAG CAGCAGCTTC AGGAATCTCA GCAGCTGCTT 
GGAAAACTGA TTCCTGAGAA ACAGTCCCTT AACGATCAAT TAAAGCAGGT TCAACAAAAC 
AGTTTGCACA GAGATTCCTT GCTGACACTA AAAAGGGCAT TGGAAGCCAA GGAGATGGGC 
CGCCAGCAGC TCCGAGACCA GTTGGATGAA GTAGAAAAAG AAACACGAGC TAAGCTCCAG 
GAAATCGATG TGTTTAATAA CCAATTAAAG GAACTGAGAG AATTGTACAA CAGACAACAG 
CTCCAGAAAC AGAAGGACTT TGAAGCCGAG AAGATCAAGC AGAAAGAGCT GGAACGGAAA 
ACCAGTGAGC TGGACAAGAT GAAAGAGGAG GACAAAAGGC GGATGCTAGA GCAGGATAAG 
CTTTGGCAGG ATCGTGTGAA GCAGGAAGAG GAACGTTACA AATTCCAAGA TGAGGAGAAG 
CAGAAAAGGG AAGAATCCGC TCAACAATGT GAGGAAGAAA AGAAACCAGA GATCCAAGAG 
AAGCCCAACA AACTGTTCCA CCTACCACCG GAGCCAGGAA AGCTAGGAGG GCATGTCCCC 
TGGATGAATA CAGAGAAAGC ACCGCTAACA TTCAACCAGG GTGATGTTAA GGTTGTGTAT 
TACCGGGCAC TCTACCCTTT TGATGCTCGA AGTCATGATG AAATCACTAT TCAGCCTGGG 
GACATTATTA TGGTGGATGA GAGCCAAACC GGAGAGCCTG GATGGCTGGG AGGTGAACTG 
AAGGGAAAAA CTGGCTGGTT TCCTGCCAAC TATGCAGAGA GAATGCCCGA GGGTGAATTC 
CCAAGCACAA CTAAACAGGC AGCAGACACT ACGGCCAAAC CCACTGTCCA TTTGGCACCT 
TCCACTCCCA CACCTGCAGC TTTTACAGAT AACTCTGCAA ATTCCAACAA CTGGGCAGAC 
TTCAGCTCCA CGTGGCCAAC CAATAACACT GATAAAGCAG AGACGGATAA CTGGGATACA 
TGGGGAGCTC AACCATCTCT GACTGTTCCT AGTGCCGGAC AGCACAGGCA GCGCTCTGCT 
TTTACCCCAG CAACGGTTAC TGGATCATCT CCATCTCCTG TACTTGGTCA GGGTGAAAAA 
GTAGAAGGGC TTCAGGCACA AGCCTTGTAT CCTTGGAGAG CAAAAAAGGA CAACCACCTT 
AATTTTAACA AAAATGACAT CATCACGGTT CTAGAGCAGC AGGATATGTG GTGGTTTGGG 
GAAGTTCAAG GTCAAAAGGG CTGGTTTCCC AAATCCTACG TAAAACTTAT ATCCGGGCCA 
CTAAGGAAAT CTACAAGCAT CGATTCCACA TCTTCAGAAA GCCCTGCTAG TTTAAAAAGA 
GTCTCTTCAC CAGCATTTAA ACCTGCCATG CAAGGAGAAG AATACATAGC CATGTACACG 
TACGAGAGCA ACGAGCAGGG AGACTTGACC TTTCAGCAAG GAGATCTGAT TGTGGTGATT 
AAAAAAGATG GGGACTGGTG GACTGGCACG GTGGGAGAGA AGACGGGGGT TTTCCCTTCC 
AACTACGTAA GGCCCAAAGA CTCCGAGGCG GCTGGAAGCG CAGGAAAGAC AGGAAGCCTT 
GGGAAGAAAC CAGAAATTGC CCAGGTTATT GCTTCTTACA CAGCAACTGG CCCTGAGCAG 
CTGACACTGG CTCCTGGCCA ACTTATCCTC ATCCGGAAAA AGAATCCTGG AGGATGGTGG 
GAAGGAGAAC TGCAGGCACG TGGGAAGAAG CGACAGATTG GATGGTTTCC AGCAAATTAT 
GTGAAGCTTT TGAGCCCTGG TACAAACAAA AGCACGCCGA CAGAACCACC AAAAGCAACA 
TCATTGCCTC CAACATGCCA AGTCATCGGG ATGTACGATT ATATCGCTCA GAATGACGAT 
GAGTTGGCCT TCGGTAAAGG TCAGGTCATT AACGTGCTGA ACAAAGAGGA CCCGGACTGG 
TGGAAAGGAG AACTGAACGG CCATGTTGGT CTGTTTCCCT CCAACTATGT GAAACTGACC 
ACAGACATGG ATCCCAGTCA GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_002939344.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939390.1 Xenopus tropicalis intersectin 1 (itsn1), transcript variant X1, mRNA.

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CloneID	OXa06414
Clone ID Related Accession (Same CDS sequence)	XM_002939344.4 , XM_002939344.5
Accession Version	XM_002939344.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intersectin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939344.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGGGATGA GTGGAATCAG TGGCATTCCT CCCCTAGCAG CTGTAGCCCC TGTGCCAATG 
CCATCCATCC CAGTAGTTGG AATGTCTCCT CCTCTTGTGT CCTCTGTCCC CACAGTGCCA 
CCCTTGGCTA ACGGAGCTCC TGCAGTAATA CAGCCTCTTC CTGCTTTCGC TCACTCTGCC 
ACGTTGCCAA AGAGTTCTTC ATTCGGCCGC TCAGGTGCCG GCTCCCAGAT GAACACTAAA 
CTTCAGAAAG CTCAGTCCTT TGATGTCCCA ACCCCCCCAC CCCTGGCAGA ATGGGCTGTA 
CCACAGTCTT CAAGGTTGAA GTACAGACAG TTGTTCAACA GCCACGACAA AACCATGAGT 
GGGCATTTAA CAGGTCCTCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCGCAGAGC 
CAGCTAGCTA CGATATGGAA CCTTTCAGAC ATTGACCTAG ATGGAAAACT AACGGCCGAA 
GAATTTATTT TAGCTATGCA CTTAATAGAT GTGGCCATGT CTGGCCAGCC ACTTCCTCCA 
GTTCTGCCTC CTGAGTATAT TCCCCCATCT TTTAGAAGAG TTCGATCGGG GAGCGGGTTA 
TCGATTATGA GCTCGGTGTC TGTAGATCAG CGCTTACCGG AGGAACCAGC AGAAGAGGAG 
CCACAGAACA CAGACAAAAG ACTTCCAGTG ACATTTGAAG ACAAGAAAAG GGAAAACTTT 
GAGCGAGGAA ACCTGGAATT AGAAAAGAGG AGACAGGCAC TTCTAGAGCA GCAGCGCAAA 
GAGCAGGAGA GGCTGGCACA GCTGGAAAGG GCAGAGCAGG AGAGAAAAGA ACGAGAGCGG 
CAAGATCAAG AGCGCAAGCG GCAGCAAGAG CTGGAAAAAC AGCTGGAGAA ACAACGAGAG 
CTGGAGCGGC AACGGGAAGA GGAGCGCAGG AAAGAAATAG AAAGGCGAGA GGCAGCAAAG 
AGAGAACTTG AAAGGCAGAG GCAGTTGGAG TGGGAACGAA ACCGGAGGCA AGAACTCCTT 
AATCAAAGGA ACAGAGAGCA AGAGGACATT GTTGTCTTAA AAGCAAAGAA AAAGACTCTT 
GAGTTTGAGC TGGAAGCTCT AAATGATAAA AAGCACCAGC TGGAAGGGAA ACTTCAGGAT 
ATCCGCTGTC GCCTCACTAC TCAACGGCAC GAAATAGAAA GCACTAACAA ATCCAGGGAG 
CTTAGGATCG CAGAGATCAC GCATTTACAG CAGCAGCTTC AGGAATCTCA GCAGCTGCTT 
GGAAAACTGA TTCCTGAGAA ACAGTCCCTT AACGATCAAT TAAAGCAGGT TCAACAAAAC 
AGTTTGCACA GAGATTCCTT GCTGACACTA AAAAGGGCAT TGGAAGCCAA GGAGATGGGC 
CGCCAGCAGC TCCGAGACCA GTTGGATGAA GTAGAAAAAG AAACACGAGC TAAGCTCCAG 
GAAATCGATG TGTTTAATAA CCAATTAAAG GAACTGAGAG AATTGTACAA CAGACAACAG 
CTCCAGAAAC AGAAGGACTT TGAAGCCGAG AAGATCAAGC AGAAAGAGCT GGAACGGAAA 
ACCAGTGAGC TGGACAAGAT GAAAGAGGAG GACAAAAGGC GGATGCTAGA GCAGGATAAG 
CTTTGGCAGG ATCGTGTGAA GCAGGAAGAG GAACGTTACA AATTCCAAGA TGAGGAGAAG 
CAGAAAAGGG AAGAATCCGC TCAACAATGT GAGGAAGAAA AGAAACCAGA GATCCAAGAG 
AAGCCCAACA AACTGTTCCA CCTACCACCG GAGCCAGGAA AGCTAGGAGG GCATGTCCCC 
TGGATGAATA CAGAGAAAGC ACCGCTAACA TTCAACCAGG GTGATGTTAA GGTTGTGTAT 
TACCGGGCAC TCTACCCTTT TGATGCTCGA AGTCATGATG AAATCACTAT TCAGCCTGGG 
GACATTATTA TGGTGGATGA GAGCCAAACC GGAGAGCCTG GATGGCTGGG AGGTGAACTG 
AAGGGAAAAA CTGGCTGGTT TCCTGCCAAC TATGCAGAGA GAATGCCCGA GGGTGAATTC 
CCAAGCACAA CTAAACAGGC AGCAGACACT ACGGCCAAAC CCACTGTCCA TTTGGCACCT 
TCCACTCCCA CACCTGCAGC TTTTACAGAT AACTCTGCAA ATTCCAACAA CTGGGCAGAC 
TTCAGCTCCA CGTGGCCAAC CAATAACACT GATAAAGCAG AGACGGATAA CTGGGATACA 
TGGGGAGCTC AACCATCTCT GACTGTTCCT AGTGCCGGAC AGCACAGGCA GCGCTCTGCT 
TTTACCCCAG CAACGGTTAC TGGATCATCT CCATCTCCTG TACTTGGTCA GGGTGAAAAA 
GTAGAAGGGC TTCAGGCACA AGCCTTGTAT CCTTGGAGAG CAAAAAAGGA CAACCACCTT 
AATTTTAACA AAAATGACAT CATCACGGTT CTAGAGCAGC AGGATATGTG GTGGTTTGGG 
GAAGTTCAAG GTCAAAAGGG CTGGTTTCCC AAATCCTACG TAAAACTTAT ATCCGGGCCA 
CTAAGGAAAT CTACAAGCAT CGATTCCACA TCTTCAGAAA GCCCTGCTAG TTTAAAAAGA 
GTCTCTTCAC CAGCATTTAA ACCTGCCATG CAAGGAGAAG AATACATAGC CATGTACACG 
TACGAGAGCA ACGAGCAGGG AGACTTGACC TTTCAGCAAG GAGATCTGAT TGTGGTGATT 
AAAAAAGATG GGGACTGGTG GACTGGCACG GTGGGAGAGA AGACGGGGGT TTTCCCTTCC 
AACTACGTAA GGCCCAAAGA CTCCGAGGCG GCTGGAAGCG CAGGAAAGAC AGGAAGCCTT 
GGGAAGAAAC CAGAAATTGC CCAGGTTATT GCTTCTTACA CAGCAACTGG CCCTGAGCAG 
CTGACACTGG CTCCTGGCCA ACTTATCCTC ATCCGGAAAA AGAATCCTGG AGGATGGTGG 
GAAGGAGAAC TGCAGGCACG TGGGAAGAAG CGACAGATTG GATGGTTTCC AGCAAATTAT 
GTGAAGCTTT TGAGCCCTGG TACAAACAAA AGCACGCCGA CAGAACCACC AAAAGCAACA 
TCATTGCCTC CAACATGCCA AGTCATCGGG ATGTACGATT ATATCGCTCA GAATGACGAT 
GAGTTGGCCT TCGGTAAAGG TCAGGTCATT AACGTGCTGA ACAAAGAGGA CCCGGACTGG 
TGGAAAGGAG AACTGAACGG CCATGTTGGT CTGTTTCCCT CCAACTATGT GAAACTGACC 
ACAGACATGG ATCCCAGTCA GCAATGGTGT GCAGACCTAC ATCTCCTGGA CATGCTGAGT 
CCCACCGAGA GGAAGAGGCA AGGGTACATC CACGAGCTCA TTGTCACCGA GGAGAACTAT 
GTCAATGACT TACAGTTAGT CACCGAGACA TTCCAAAAGC CTCTGCTGGA ATCTGAATTA 
CTGACGGAGA AGGAAGTTGC CATGATTTTT GTCAACTGGA AGGAGCTGAT AATGTGCAAT 
ATCAAACTGC TCAAGGCCTT GCGAGTTCGC AAGAAAATGT CTGGGGAGAA GATGCCGGTG 
AAGATGATCG GGGACATCCT GACGGCCCAG TTGCCCCACA TGCAGCCGTA CATTCGCTTC 
TGCAGCTGCC AGCTCAACGG CGCCGCTCTC ATTCAGCAGA AAACGGACGA GGTGCCCGAG 
TTCAAGGAGT TTGTGAAAAG GCTTGCAATG GATCCCCGGT GTAAGGGAAT GCCGCTGTCC 
AGCTTCCTAC TCAAACCCAT GCAGAGAGTA ACCAGATACC CGCTCATCAT TAAAAATATT 
ATTGAAAATA CTCCAGAAAA TCACCCTGAC CACAGCCACC TGAAGCAAGC CCTAGAGAAA 
GCCGAAGAAT TATGTTCCCA AGTGAATGAG GGGGTTCGGG AGAAGGAAAA CTCAGACCGA 
CTGGAGTGGA TTCAGGCGCA CGTTCAGTGT GAGGGTTTGT CTGAGCAATT AGTATTTAAC 
TCTGTTACAA ACTGCCTGGG CCCACGCAAG TTCCTGCACA GTGGGAAACT GTACAAAGCC 
AAGAGCAACA AGGAACTATA CGGCTTCCTG TTCAACGACT TCCTGCTGCT CACCCAGATC 
ATTAAGCCCC TCGGCTCCTC CGGCAACGAT AAGGTGTTCA GCCCCAAGTC CAATCTCCAG 
TACAAGATGT ACAAAACTCC AATATTCCTC AACGAAGTGC TGGTGAAACT GCCCACGGAC 
CCATCCGGGG ATGAGCCAAT CTTTCATATT TCTCACATTG ACCGAGTCTA CACATTGCGT 
GCAGAGAGCA TCAATGAGAG GACGGCCTGG GTGCAGAAAA TCAAAGCGGC ATCGGAGTTG 
TACATAGAGA CGGAGAAGAA GAAGAGAGAG AAGGCCTACT TGGTTCGCTC CCAACGCGCA 
ACAGGCATCG GAAGACTGAT GGTAAATATC GTTGAGGGAA TTGAGTTGAA GCCCTGCAGA 
ACCCACGGGA AGAGCAATCC TTACTGTGAG ATCACCATGG GCTCCCAGTG CCACATTACC 
AAGACCATAC AGGACACCCT GAACCCCAAG TGGAACTCCA ACTGCCAATT CTTTATCAAG 
GACCTGGAGC AGGACGTTCT GTGCATCACC GTGTTTGAGA GGGACCAGTT TTCCCCTGAC 
GATTTCCTGG GCCGGACAGA AATCCGAGTT GCTGACATAA AGAAAGACCA AGGTTCTAAA 
GGACCAGTAA CCAAGTGTCT ACTTCTGCAC GAAGTTCCTA CTGGAGAGAT AGTGGTCCGA 
TTGGATCTGC AGCTGTTTGA CGAGCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939390.1
CDS	267..5396
Translation	MAQFGTPFGGNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSAAAAAAAAAGFGMSGISGIPPLAAVAPVPMPSIPVVGMSPPLVSSVPTVPPLANGAPAVIQPLPAFAHSATLPKSSSFGRSGAGSQMNTKLQKAQSFDVPTPPPLAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGLSIMSSVSVDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRELRIAEITHLQQQLQESQQLLGKLIPEKQSLNDQLKQVQQNSLHRDSLLTLKRALEAKEMGRQQLRDQLDEVEKETRAKLQEIDVFNNQLKELRELYNRQQLQKQKDFEAEKIKQKELERKTSELDKMKEEDKRRMLEQDKLWQDRVKQEEERYKFQDEEKQKREESAQQCEEEKKPEIQEKPNKLFHLPPEPGKLGGHVPWMNTEKAPLTFNQGDVKVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEPGWLGGELKGKTGWFPANYAERMPEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTWPTNNTDKAETDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVTGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRVSSPAFKPAMQGEEYIAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTNKSTPTEPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGELNGHVGLFPSNYVKLTTDMDPSQQWCADLHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_002939344.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939344.5

ORF Insert Sequence:

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGGGATGA GTGGAATCAG TGGCATTCCT CCCCTAGCAG CTGTAGCCCC TGTGCCAATG 
CCATCCATCC CAGTAGTTGG AATGTCTCCT CCTCTTGTGT CCTCTGTCCC CACAGTGCCA 
CCCTTGGCTA ACGGAGCTCC TGCAGTAATA CAGCCTCTTC CTGCTTTCGC TCACTCTGCC 
ACGTTGCCAA AGAGTTCTTC ATTCGGCCGC TCAGGTGCCG GCTCCCAGAT GAACACTAAA 
CTTCAGAAAG CTCAGTCCTT TGATGTCCCA ACCCCCCCAC CCCTGGCAGA ATGGGCTGTA 
CCACAGTCTT CAAGGTTGAA GTACAGACAG TTGTTCAACA GCCACGACAA AACCATGAGT 
GGGCATTTAA CAGGTCCTCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCGCAGAGC 
CAGCTAGCTA CGATATGGAA CCTTTCAGAC ATTGACCTAG ATGGAAAACT AACGGCCGAA 
GAATTTATTT TAGCTATGCA CTTAATAGAT GTGGCCATGT CTGGCCAGCC ACTTCCTCCA 
GTTCTGCCTC CTGAGTATAT TCCCCCATCT TTTAGAAGAG TTCGATCGGG GAGCGGGTTA 
TCGATTATGA GCTCGGTGTC TGTAGATCAG CGCTTACCGG AGGAACCAGC AGAAGAGGAG 
CCACAGAACA CAGACAAAAG ACTTCCAGTG ACATTTGAAG ACAAGAAAAG GGAAAACTTT 
GAGCGAGGAA ACCTGGAATT AGAAAAGAGG AGACAGGCAC TTCTAGAGCA GCAGCGCAAA 
GAGCAGGAGA GGCTGGCACA GCTGGAAAGG GCAGAGCAGG AGAGAAAAGA ACGAGAGCGG 
CAAGATCAAG AGCGCAAGCG GCAGCAAGAG CTGGAAAAAC AGCTGGAGAA ACAACGAGAG 
CTGGAGCGGC AACGGGAAGA GGAGCGCAGG AAAGAAATAG AAAGGCGAGA GGCAGCAAAG 
AGAGAACTTG AAAGGCAGAG GCAGTTGGAG TGGGAACGAA ACCGGAGGCA AGAACTCCTT 
AATCAAAGGA ACAGAGAGCA AGAGGACATT GTTGTCTTAA AAGCAAAGAA AAAGACTCTT 
GAGTTTGAGC TGGAAGCTCT AAATGATAAA AAGCACCAGC TGGAAGGGAA ACTTCAGGAT 
ATCCGCTGTC GCCTCACTAC TCAACGGCAC GAAATAGAAA GCACTAACAA ATCCAGGGAG 
CTTAGGATCG CAGAGATCAC GCATTTACAG CAGCAGCTTC AGGAATCTCA GCAGCTGCTT 
GGAAAACTGA TTCCTGAGAA ACAGTCCCTT AACGATCAAT TAAAGCAGGT TCAACAAAAC 
AGTTTGCACA GAGATTCCTT GCTGACACTA AAAAGGGCAT TGGAAGCCAA GGAGATGGGC 
CGCCAGCAGC TCCGAGACCA GTTGGATGAA GTAGAAAAAG AAACACGAGC TAAGCTCCAG 
GAAATCGATG TGTTTAATAA CCAATTAAAG GAACTGAGAG AATTGTACAA CAGACAACAG 
CTCCAGAAAC AGAAGGACTT TGAAGCCGAG AAGATCAAGC AGAAAGAGCT GGAACGGAAA 
ACCAGTGAGC TGGACAAGAT GAAAGAGGAG GACAAAAGGC GGATGCTAGA GCAGGATAAG 
CTTTGGCAGG ATCGTGTGAA GCAGGAAGAG GAACGTTACA AATTCCAAGA TGAGGAGAAG 
CAGAAAAGGG AAGAATCCGC TCAACAATGT GAGGAAGAAA AGAAACCAGA GATCCAAGAG 
AAGCCCAACA AACTGTTCCA CCTACCACCG GAGCCAGGAA AGCTAGGAGG GCATGTCCCC 
TGGATGAATA CAGAGAAAGC ACCGCTAACA TTCAACCAGG GTGATGTTAA GGTTGTGTAT 
TACCGGGCAC TCTACCCTTT TGATGCTCGA AGTCATGATG AAATCACTAT TCAGCCTGGG 
GACATTATTA TGGTGGATGA GAGCCAAACC GGAGAGCCTG GATGGCTGGG AGGTGAACTG 
AAGGGAAAAA CTGGCTGGTT TCCTGCCAAC TATGCAGAGA GAATGCCCGA GGGTGAATTC 
CCAAGCACAA CTAAACAGGC AGCAGACACT ACGGCCAAAC CCACTGTCCA TTTGGCACCT 
TCCACTCCCA CACCTGCAGC TTTTACAGAT AACTCTGCAA ATTCCAACAA CTGGGCAGAC 
TTCAGCTCCA CGTGGCCAAC CAATAACACT GATAAAGCAG AGACGGATAA CTGGGATACA 
TGGGGAGCTC AACCATCTCT GACTGTTCCT AGTGCCGGAC AGCACAGGCA GCGCTCTGCT 
TTTACCCCAG CAACGGTTAC TGGATCATCT CCATCTCCTG TACTTGGTCA GGGTGAAAAA 
GTAGAAGGGC TTCAGGCACA AGCCTTGTAT CCTTGGAGAG CAAAAAAGGA CAACCACCTT 
AATTTTAACA AAAATGACAT CATCACGGTT CTAGAGCAGC AGGATATGTG GTGGTTTGGG 
GAAGTTCAAG GTCAAAAGGG CTGGTTTCCC AAATCCTACG TAAAACTTAT ATCCGGGCCA 
CTAAGGAAAT CTACAAGCAT CGATTCCACA TCTTCAGAAA GCCCTGCTAG TTTAAAAAGA 
GTCTCTTCAC CAGCATTTAA ACCTGCCATG CAAGGAGAAG AATACATAGC CATGTACACG 
TACGAGAGCA ACGAGCAGGG AGACTTGACC TTTCAGCAAG GAGATCTGAT TGTGGTGATT 
AAAAAAGATG GGGACTGGTG GACTGGCACG GTGGGAGAGA AGACGGGGGT TTTCCCTTCC 
AACTACGTAA GGCCCAAAGA CTCCGAGGCG GCTGGAAGCG CAGGAAAGAC AGGAAGCCTT 
GGGAAGAAAC CAGAAATTGC CCAGGTTATT GCTTCTTACA CAGCAACTGG CCCTGAGCAG 
CTGACACTGG CTCCTGGCCA ACTTATCCTC ATCCGGAAAA AGAATCCTGG AGGATGGTGG 
GAAGGAGAAC TGCAGGCACG TGGGAAGAAG CGACAGATTG GATGGTTTCC AGCAAATTAT 
GTGAAGCTTT TGAGCCCTGG TACAAACAAA AGCACGCCGA CAGAACCACC AAAAGCAACA 
TCATTGCCTC CAACATGCCA AGTCATCGGG ATGTACGATT ATATCGCTCA GAATGACGAT 
GAGTTGGCCT TCGGTAAAGG TCAGGTCATT AACGTGCTGA ACAAAGAGGA CCCGGACTGG 
TGGAAAGGAG AACTGAACGG CCATGTTGGT CTGTTTCCCT CCAACTATGT GAAACTGACC 
ACAGACATGG ATCCCAGTCA GCAATGGTGT GCAGACCTAC ATCTCCTGGA CATGCTGAGT 
CCCACCGAGA GGAAGAGGCA AGGGTACATC CACGAGCTCA TTGTCACCGA GGAGAACTAT 
GTCAATGACT TACAGTTAGT CACCGAGACA TTCCAAAAGC CTCTGCTGGA ATCTGAATTA 
CTGACGGAGA AGGAAGTTGC CATGATTTTT GTCAACTGGA AGGAGCTGAT AATGTGCAAT 
ATCAAACTGC TCAAGGCCTT GCGAGTTCGC AAGAAAATGT CTGGGGAGAA GATGCCGGTG 
AAGATGATCG GGGACATCCT GACGGCCCAG TTGCCCCACA TGCAGCCGTA CATTCGCTTC 
TGCAGCTGCC AGCTCAACGG CGCCGCTCTC ATTCAGCAGA AAACGGACGA GGTGCCCGAG 
TTCAAGGAGT TTGTGAAAAG GCTTGCAATG GATCCCCGGT GTAAGGGAAT GCCGCTGTCC 
AGCTTCCTAC TCAAACCCAT GCAGAGAGTA ACCAGATACC CGCTCATCAT TAAAAATATT 
ATTGAAAATA CTCCAGAAAA TCACCCTGAC CACAGCCACC TGAAGCAAGC CCTAGAGAAA 
GCCGAAGAAT TATGTTCCCA AGTGAATGAG GGGGTTCGGG AGAAGGAAAA CTCAGACCGA 
CTGGAGTGGA TTCAGGCGCA CGTTCAGTGT GAGGGTTTGT CTGAGCAATT AGTATTTAAC 
TCTGTTACAA ACTGCCTGGG CCCACGCAAG TTCCTGCACA GTGGGAAACT GTACAAAGCC 
AAGAGCAACA AGGAACTATA CGGCTTCCTG TTCAACGACT TCCTGCTGCT CACCCAGATC 
ATTAAGCCCC TCGGCTCCTC CGGCAACGAT AAGGTGTTCA GCCCCAAGTC CAATCTCCAG 
TACAAGATGT ACAAAACTCC AATATTCCTC AACGAAGTGC TGGTGAAACT GCCCACGGAC 
CCATCCGGGG ATGAGCCAAT CTTTCATATT TCTCACATTG ACCGAGTCTA CACATTGCGT 
GCAGAGAGCA TCAATGAGAG GACGGCCTGG GTGCAGAAAA TCAAAGCGGC ATCGGAGTTG 
TACATAGAGA CGGAGAAGAA GAAGAGAGAG AAGGCCTACT TGGTTCGCTC CCAACGCGCA 
ACAGGCATCG GAAGACTGAT GGTAAATATC GTTGAGGGAA TTGAGTTGAA GCCCTGCAGA 
ACCCACGGGA AGAGCAATCC TTACTGTGAG ATCACCATGG GCTCCCAGTG CCACATTACC 
AAGACCATAC AGGACACCCT GAACCCCAAG TGGAACTCCA ACTGCCAATT CTTTATCAAG 
GACCTGGAGC AGGACGTTCT GTGCATCACC GTGTTTGAGA GGGACCAGTT TTCCCCTGAC 
GATTTCCTGG GCCGGACAGA AATCCGAGTT GCTGACATAA AGAAAGACCA AGGTTCTAAA 
GGACCAGTAA CCAAGTGTCT ACTTCTGCAC GAAGTTCCTA CTGGAGAGAT AGTGGTCCGA 
TTGGATCTGC AGCTGTTTGA CGAGCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_004912127.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912184.1 Xenopus tropicalis intersectin 1 (itsn1), transcript variant X2, mRNA.

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CloneID	OXa06415
Clone ID Related Accession (Same CDS sequence)	XM_004912127.3 , XM_004912127.4
Accession Version	XM_004912127.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intersectin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912127.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGGGATGA GTGGAATCAG TGGCATTCCT CCCCTAGCAG CTGTAGCCCC TGTGCCAATG 
CCATCCATCC CAGTAGTTGG AATGTCTCCT CCTCTTGTGT CCTCTGTCCC CACAGTGCCA 
CCCTTGGCTA ACGGAGCTCC TGCAGTAATA CAGCCTCTTC CTGCTTTCGC TCACTCTGCC 
ACGTTGCCAA AGAGTTCTTC ATTCGGCCGC TCAGGTGCCG GCTCCCAGAT GAACACTAAA 
CTTCAGAAAG CTCAGTCCTT TGATGTCCCA ACCCCCCCAC CCCTGGCAGA ATGGGCTGTA 
CCACAGTCTT CAAGGTTGAA GTACAGACAG TTGTTCAACA GCCACGACAA AACCATGAGT 
GGGCATTTAA CAGGTCCTCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCGCAGAGC 
CAGCTAGCTA CGATATGGAA CCTTTCAGAC ATTGACCTAG ATGGAAAACT AACGGCCGAA 
GAATTTATTT TAGCTATGCA CTTAATAGAT GTGGCCATGT CTGGCCAGCC ACTTCCTCCA 
GTTCTGCCTC CTGAGTATAT TCCCCCATCT TTTAGAGTTC GATCGGGGAG CGGGTTATCG 
ATTATGAGCT CGGTGTCTGT AGATCAGCGC TTACCGGAGG AACCAGCAGA AGAGGAGCCA 
CAGAACACAG ACAAAAGACT TCCAGTGACA TTTGAAGACA AGAAAAGGGA AAACTTTGAG 
CGAGGAAACC TGGAATTAGA AAAGAGGAGA CAGGCACTTC TAGAGCAGCA GCGCAAAGAG 
CAGGAGAGGC TGGCACAGCT GGAAAGGGCA GAGCAGGAGA GAAAAGAACG AGAGCGGCAA 
GATCAAGAGC GCAAGCGGCA GCAAGAGCTG GAAAAACAGC TGGAGAAACA ACGAGAGCTG 
GAGCGGCAAC GGGAAGAGGA GCGCAGGAAA GAAATAGAAA GGCGAGAGGC AGCAAAGAGA 
GAACTTGAAA GGCAGAGGCA GTTGGAGTGG GAACGAAACC GGAGGCAAGA ACTCCTTAAT 
CAAAGGAACA GAGAGCAAGA GGACATTGTT GTCTTAAAAG CAAAGAAAAA GACTCTTGAG 
TTTGAGCTGG AAGCTCTAAA TGATAAAAAG CACCAGCTGG AAGGGAAACT TCAGGATATC 
CGCTGTCGCC TCACTACTCA ACGGCACGAA ATAGAAAGCA CTAACAAATC CAGGGAGCTT 
AGGATCGCAG AGATCACGCA TTTACAGCAG CAGCTTCAGG AATCTCAGCA GCTGCTTGGA 
AAACTGATTC CTGAGAAACA GTCCCTTAAC GATCAATTAA AGCAGGTTCA ACAAAACAGT 
TTGCACAGAG ATTCCTTGCT GACACTAAAA AGGGCATTGG AAGCCAAGGA GATGGGCCGC 
CAGCAGCTCC GAGACCAGTT GGATGAAGTA GAAAAAGAAA CACGAGCTAA GCTCCAGGAA 
ATCGATGTGT TTAATAACCA ATTAAAGGAA CTGAGAGAAT TGTACAACAG ACAACAGCTC 
CAGAAACAGA AGGACTTTGA AGCCGAGAAG ATCAAGCAGA AAGAGCTGGA ACGGAAAACC 
AGTGAGCTGG ACAAGATGAA AGAGGAGGAC AAAAGGCGGA TGCTAGAGCA GGATAAGCTT 
TGGCAGGATC GTGTGAAGCA GGAAGAGGAA CGTTACAAAT TCCAAGATGA GGAGAAGCAG 
AAAAGGGAAG AATCCGCTCA ACAATGTGAG GAAGAAAAGA AACCAGAGAT CCAAGAGAAG 
CCCAACAAAC TGTTCCACCT ACCACCGGAG CCAGGAAAGC TAGGAGGGCA TGTCCCCTGG 
ATGAATACAG AGAAAGCACC GCTAACATTC AACCAGGGTG ATGTTAAGGT TGTGTATTAC 
CGGGCACTCT ACCCTTTTGA TGCTCGAAGT CATGATGAAA TCACTATTCA GCCTGGGGAC 
ATTATTATGG TGGATGAGAG CCAAACCGGA GAGCCTGGAT GGCTGGGAGG TGAACTGAAG 
GGAAAAACTG GCTGGTTTCC TGCCAACTAT GCAGAGAGAA TGCCCGAGGG TGAATTCCCA 
AGCACAACTA AACAGGCAGC AGACACTACG GCCAAACCCA CTGTCCATTT GGCACCTTCC 
ACTCCCACAC CTGCAGCTTT TACAGATAAC TCTGCAAATT CCAACAACTG GGCAGACTTC 
AGCTCCACGT GGCCAACCAA TAACACTGAT AAAGCAGAGA CGGATAACTG GGATACATGG 
GGAGCTCAAC CATCTCTGAC TGTTCCTAGT GCCGGACAGC ACAGGCAGCG CTCTGCTTTT 
ACCCCAGCAA CGGTTACTGG ATCATCTCCA TCTCCTGTAC TTGGTCAGGG TGAAAAAGTA 
GAAGGGCTTC AGGCACAAGC CTTGTATCCT TGGAGAGCAA AAAAGGACAA CCACCTTAAT 
TTTAACAAAA ATGACATCAT CACGGTTCTA GAGCAGCAGG ATATGTGGTG GTTTGGGGAA 
GTTCAAGGTC AAAAGGGCTG GTTTCCCAAA TCCTACGTAA AACTTATATC CGGGCCACTA 
AGGAAATCTA CAAGCATCGA TTCCACATCT TCAGAAAGCC CTGCTAGTTT AAAAAGAGTC 
TCTTCACCAG CATTTAAACC TGCCATGCAA GGAGAAGAAT ACATAGCCAT GTACACGTAC 
GAGAGCAACG AGCAGGGAGA CTTGACCTTT CAGCAAGGAG ATCTGATTGT GGTGATTAAA 
AAAGATGGGG ACTGGTGGAC TGGCACGGTG GGAGAGAAGA CGGGGGTTTT CCCTTCCAAC 
TACGTAAGGC CCAAAGACTC CGAGGCGGCT GGAAGCGCAG GAAAGACAGG AAGCCTTGGG 
AAGAAACCAG AAATTGCCCA GGTTATTGCT TCTTACACAG CAACTGGCCC TGAGCAGCTG 
ACACTGGCTC CTGGCCAACT TATCCTCATC CGGAAAAAGA ATCCTGGAGG ATGGTGGGAA 
GGAGAACTGC AGGCACGTGG GAAGAAGCGA CAGATTGGAT GGTTTCCAGC AAATTATGTG 
AAGCTTTTGA GCCCTGGTAC AAACAAAAGC ACGCCGACAG AACCACCAAA AGCAACATCA 
TTGCCTCCAA CATGCCAAGT CATCGGGATG TACGATTATA TCGCTCAGAA TGACGATGAG 
TTGGCCTTCG GTAAAGGTCA GGTCATTAAC GTGCTGAACA AAGAGGACCC GGACTGGTGG 
AAAGGAGAAC TGAACGGCCA TGTTGGTCTG TTTCCCTCCA ACTATGTGAA ACTGACCACA 
GACATGGATC CCAGTCAGCA ATGGTGTGCA GACCTACATC TCCTGGACAT GCTGAGTCCC 
ACCGAGAGGA AGAGGCAAGG GTACATCCAC GAGCTCATTG TCACCGAGGA GAACTATGTC 
AATGACTTAC AGTTAGTCAC CGAGACATTC CAAAAGCCTC TGCTGGAATC TGAATTACTG 
ACGGAGAAGG AAGTTGCCAT GATTTTTGTC AACTGGAAGG AGCTGATAAT GTGCAATATC 
AAACTGCTCA AGGCCTTGCG AGTTCGCAAG AAAATGTCTG GGGAGAAGAT GCCGGTGAAG 
ATGATCGGGG ACATCCTGAC GGCCCAGTTG CCCCACATGC AGCCGTACAT TCGCTTCTGC 
AGCTGCCAGC TCAACGGCGC CGCTCTCATT CAGCAGAAAA CGGACGAGGT GCCCGAGTTC 
AAGGAGTTTG TGAAAAGGCT TGCAATGGAT CCCCGGTGTA AGGGAATGCC GCTGTCCAGC 
TTCCTACTCA AACCCATGCA GAGAGTAACC AGATACCCGC TCATCATTAA AAATATTATT 
GAAAATACTC CAGAAAATCA CCCTGACCAC AGCCACCTGA AGCAAGCCCT AGAGAAAGCC 
GAAGAATTAT GTTCCCAAGT GAATGAGGGG GTTCGGGAGA AGGAAAACTC AGACCGACTG 
GAGTGGATTC AGGCGCACGT TCAGTGTGAG GGTTTGTCTG AGCAATTAGT ATTTAACTCT 
GTTACAAACT GCCTGGGCCC ACGCAAGTTC CTGCACAGTG GGAAACTGTA CAAAGCCAAG 
AGCAACAAGG AACTATACGG CTTCCTGTTC AACGACTTCC TGCTGCTCAC CCAGATCATT 
AAGCCCCTCG GCTCCTCCGG CAACGATAAG GTGTTCAGCC CCAAGTCCAA TCTCCAGTAC 
AAGATGTACA AAACTCCAAT ATTCCTCAAC GAAGTGCTGG TGAAACTGCC CACGGACCCA 
TCCGGGGATG AGCCAATCTT TCATATTTCT CACATTGACC GAGTCTACAC ATTGCGTGCA 
GAGAGCATCA ATGAGAGGAC GGCCTGGGTG CAGAAAATCA AAGCGGCATC GGAGTTGTAC 
ATAGAGACGG AGAAGAAGAA GAGAGAGAAG GCCTACTTGG TTCGCTCCCA ACGCGCAACA 
GGCATCGGAA GACTGATGGT AAATATCGTT GAGGGAATTG AGTTGAAGCC CTGCAGAACC 
CACGGGAAGA GCAATCCTTA CTGTGAGATC ACCATGGGCT CCCAGTGCCA CATTACCAAG 
ACCATACAGG ACACCCTGAA CCCCAAGTGG AACTCCAACT GCCAATTCTT TATCAAGGAC 
CTGGAGCAGG ACGTTCTGTG CATCACCGTG TTTGAGAGGG ACCAGTTTTC CCCTGACGAT 
TTCCTGGGCC GGACAGAAAT CCGAGTTGCT GACATAAAGA AAGACCAAGG TTCTAAAGGA 
CCAGTAACCA AGTGTCTACT TCTGCACGAA GTTCCTACTG GAGAGATAGT GGTCCGATTG 
GATCTGCAGC TGTTTGACGA GCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912184.1
CDS	267..5393
Translation	MAQFGTPFGGNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSAAAAAAAAAGFGMSGISGIPPLAAVAPVPMPSIPVVGMSPPLVSSVPTVPPLANGAPAVIQPLPAFAHSATLPKSSSFGRSGAGSQMNTKLQKAQSFDVPTPPPLAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRVRSGSGLSIMSSVSVDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRELRIAEITHLQQQLQESQQLLGKLIPEKQSLNDQLKQVQQNSLHRDSLLTLKRALEAKEMGRQQLRDQLDEVEKETRAKLQEIDVFNNQLKELRELYNRQQLQKQKDFEAEKIKQKELERKTSELDKMKEEDKRRMLEQDKLWQDRVKQEEERYKFQDEEKQKREESAQQCEEEKKPEIQEKPNKLFHLPPEPGKLGGHVPWMNTEKAPLTFNQGDVKVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEPGWLGGELKGKTGWFPANYAERMPEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTWPTNNTDKAETDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVTGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRVSSPAFKPAMQGEEYIAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTNKSTPTEPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGELNGHVGLFPSNYVKLTTDMDPSQQWCADLHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_004912127.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912127.4

ORF Insert Sequence:

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGGGATGA GTGGAATCAG TGGCATTCCT CCCCTAGCAG CTGTAGCCCC TGTGCCAATG 
CCATCCATCC CAGTAGTTGG AATGTCTCCT CCTCTTGTGT CCTCTGTCCC CACAGTGCCA 
CCCTTGGCTA ACGGAGCTCC TGCAGTAATA CAGCCTCTTC CTGCTTTCGC TCACTCTGCC 
ACGTTGCCAA AGAGTTCTTC ATTCGGCCGC TCAGGTGCCG GCTCCCAGAT GAACACTAAA 
CTTCAGAAAG CTCAGTCCTT TGATGTCCCA ACCCCCCCAC CCCTGGCAGA ATGGGCTGTA 
CCACAGTCTT CAAGGTTGAA GTACAGACAG TTGTTCAACA GCCACGACAA AACCATGAGT 
GGGCATTTAA CAGGTCCTCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCGCAGAGC 
CAGCTAGCTA CGATATGGAA CCTTTCAGAC ATTGACCTAG ATGGAAAACT AACGGCCGAA 
GAATTTATTT TAGCTATGCA CTTAATAGAT GTGGCCATGT CTGGCCAGCC ACTTCCTCCA 
GTTCTGCCTC CTGAGTATAT TCCCCCATCT TTTAGAGTTC GATCGGGGAG CGGGTTATCG 
ATTATGAGCT CGGTGTCTGT AGATCAGCGC TTACCGGAGG AACCAGCAGA AGAGGAGCCA 
CAGAACACAG ACAAAAGACT TCCAGTGACA TTTGAAGACA AGAAAAGGGA AAACTTTGAG 
CGAGGAAACC TGGAATTAGA AAAGAGGAGA CAGGCACTTC TAGAGCAGCA GCGCAAAGAG 
CAGGAGAGGC TGGCACAGCT GGAAAGGGCA GAGCAGGAGA GAAAAGAACG AGAGCGGCAA 
GATCAAGAGC GCAAGCGGCA GCAAGAGCTG GAAAAACAGC TGGAGAAACA ACGAGAGCTG 
GAGCGGCAAC GGGAAGAGGA GCGCAGGAAA GAAATAGAAA GGCGAGAGGC AGCAAAGAGA 
GAACTTGAAA GGCAGAGGCA GTTGGAGTGG GAACGAAACC GGAGGCAAGA ACTCCTTAAT 
CAAAGGAACA GAGAGCAAGA GGACATTGTT GTCTTAAAAG CAAAGAAAAA GACTCTTGAG 
TTTGAGCTGG AAGCTCTAAA TGATAAAAAG CACCAGCTGG AAGGGAAACT TCAGGATATC 
CGCTGTCGCC TCACTACTCA ACGGCACGAA ATAGAAAGCA CTAACAAATC CAGGGAGCTT 
AGGATCGCAG AGATCACGCA TTTACAGCAG CAGCTTCAGG AATCTCAGCA GCTGCTTGGA 
AAACTGATTC CTGAGAAACA GTCCCTTAAC GATCAATTAA AGCAGGTTCA ACAAAACAGT 
TTGCACAGAG ATTCCTTGCT GACACTAAAA AGGGCATTGG AAGCCAAGGA GATGGGCCGC 
CAGCAGCTCC GAGACCAGTT GGATGAAGTA GAAAAAGAAA CACGAGCTAA GCTCCAGGAA 
ATCGATGTGT TTAATAACCA ATTAAAGGAA CTGAGAGAAT TGTACAACAG ACAACAGCTC 
CAGAAACAGA AGGACTTTGA AGCCGAGAAG ATCAAGCAGA AAGAGCTGGA ACGGAAAACC 
AGTGAGCTGG ACAAGATGAA AGAGGAGGAC AAAAGGCGGA TGCTAGAGCA GGATAAGCTT 
TGGCAGGATC GTGTGAAGCA GGAAGAGGAA CGTTACAAAT TCCAAGATGA GGAGAAGCAG 
AAAAGGGAAG AATCCGCTCA ACAATGTGAG GAAGAAAAGA AACCAGAGAT CCAAGAGAAG 
CCCAACAAAC TGTTCCACCT ACCACCGGAG CCAGGAAAGC TAGGAGGGCA TGTCCCCTGG 
ATGAATACAG AGAAAGCACC GCTAACATTC AACCAGGGTG ATGTTAAGGT TGTGTATTAC 
CGGGCACTCT ACCCTTTTGA TGCTCGAAGT CATGATGAAA TCACTATTCA GCCTGGGGAC 
ATTATTATGG TGGATGAGAG CCAAACCGGA GAGCCTGGAT GGCTGGGAGG TGAACTGAAG 
GGAAAAACTG GCTGGTTTCC TGCCAACTAT GCAGAGAGAA TGCCCGAGGG TGAATTCCCA 
AGCACAACTA AACAGGCAGC AGACACTACG GCCAAACCCA CTGTCCATTT GGCACCTTCC 
ACTCCCACAC CTGCAGCTTT TACAGATAAC TCTGCAAATT CCAACAACTG GGCAGACTTC 
AGCTCCACGT GGCCAACCAA TAACACTGAT AAAGCAGAGA CGGATAACTG GGATACATGG 
GGAGCTCAAC CATCTCTGAC TGTTCCTAGT GCCGGACAGC ACAGGCAGCG CTCTGCTTTT 
ACCCCAGCAA CGGTTACTGG ATCATCTCCA TCTCCTGTAC TTGGTCAGGG TGAAAAAGTA 
GAAGGGCTTC AGGCACAAGC CTTGTATCCT TGGAGAGCAA AAAAGGACAA CCACCTTAAT 
TTTAACAAAA ATGACATCAT CACGGTTCTA GAGCAGCAGG ATATGTGGTG GTTTGGGGAA 
GTTCAAGGTC AAAAGGGCTG GTTTCCCAAA TCCTACGTAA AACTTATATC CGGGCCACTA 
AGGAAATCTA CAAGCATCGA TTCCACATCT TCAGAAAGCC CTGCTAGTTT AAAAAGAGTC 
TCTTCACCAG CATTTAAACC TGCCATGCAA GGAGAAGAAT ACATAGCCAT GTACACGTAC 
GAGAGCAACG AGCAGGGAGA CTTGACCTTT CAGCAAGGAG ATCTGATTGT GGTGATTAAA 
AAAGATGGGG ACTGGTGGAC TGGCACGGTG GGAGAGAAGA CGGGGGTTTT CCCTTCCAAC 
TACGTAAGGC CCAAAGACTC CGAGGCGGCT GGAAGCGCAG GAAAGACAGG AAGCCTTGGG 
AAGAAACCAG AAATTGCCCA GGTTATTGCT TCTTACACAG CAACTGGCCC TGAGCAGCTG 
ACACTGGCTC CTGGCCAACT TATCCTCATC CGGAAAAAGA ATCCTGGAGG ATGGTGGGAA 
GGAGAACTGC AGGCACGTGG GAAGAAGCGA CAGATTGGAT GGTTTCCAGC AAATTATGTG 
AAGCTTTTGA GCCCTGGTAC AAACAAAAGC ACGCCGACAG AACCACCAAA AGCAACATCA 
TTGCCTCCAA CATGCCAAGT CATCGGGATG TACGATTATA TCGCTCAGAA TGACGATGAG 
TTGGCCTTCG GTAAAGGTCA GGTCATTAAC GTGCTGAACA AAGAGGACCC GGACTGGTGG 
AAAGGAGAAC TGAACGGCCA TGTTGGTCTG TTTCCCTCCA ACTATGTGAA ACTGACCACA 
GACATGGATC CCAGTCAGCA ATGGTGTGCA GACCTACATC TCCTGGACAT GCTGAGTCCC 
ACCGAGAGGA AGAGGCAAGG GTACATCCAC GAGCTCATTG TCACCGAGGA GAACTATGTC 
AATGACTTAC AGTTAGTCAC CGAGACATTC CAAAAGCCTC TGCTGGAATC TGAATTACTG 
ACGGAGAAGG AAGTTGCCAT GATTTTTGTC AACTGGAAGG AGCTGATAAT GTGCAATATC 
AAACTGCTCA AGGCCTTGCG AGTTCGCAAG AAAATGTCTG GGGAGAAGAT GCCGGTGAAG 
ATGATCGGGG ACATCCTGAC GGCCCAGTTG CCCCACATGC AGCCGTACAT TCGCTTCTGC 
AGCTGCCAGC TCAACGGCGC CGCTCTCATT CAGCAGAAAA CGGACGAGGT GCCCGAGTTC 
AAGGAGTTTG TGAAAAGGCT TGCAATGGAT CCCCGGTGTA AGGGAATGCC GCTGTCCAGC 
TTCCTACTCA AACCCATGCA GAGAGTAACC AGATACCCGC TCATCATTAA AAATATTATT 
GAAAATACTC CAGAAAATCA CCCTGACCAC AGCCACCTGA AGCAAGCCCT AGAGAAAGCC 
GAAGAATTAT GTTCCCAAGT GAATGAGGGG GTTCGGGAGA AGGAAAACTC AGACCGACTG 
GAGTGGATTC AGGCGCACGT TCAGTGTGAG GGTTTGTCTG AGCAATTAGT ATTTAACTCT 
GTTACAAACT GCCTGGGCCC ACGCAAGTTC CTGCACAGTG GGAAACTGTA CAAAGCCAAG 
AGCAACAAGG AACTATACGG CTTCCTGTTC AACGACTTCC TGCTGCTCAC CCAGATCATT 
AAGCCCCTCG GCTCCTCCGG CAACGATAAG GTGTTCAGCC CCAAGTCCAA TCTCCAGTAC 
AAGATGTACA AAACTCCAAT ATTCCTCAAC GAAGTGCTGG TGAAACTGCC CACGGACCCA 
TCCGGGGATG AGCCAATCTT TCATATTTCT CACATTGACC GAGTCTACAC ATTGCGTGCA 
GAGAGCATCA ATGAGAGGAC GGCCTGGGTG CAGAAAATCA AAGCGGCATC GGAGTTGTAC 
ATAGAGACGG AGAAGAAGAA GAGAGAGAAG GCCTACTTGG TTCGCTCCCA ACGCGCAACA 
GGCATCGGAA GACTGATGGT AAATATCGTT GAGGGAATTG AGTTGAAGCC CTGCAGAACC 
CACGGGAAGA GCAATCCTTA CTGTGAGATC ACCATGGGCT CCCAGTGCCA CATTACCAAG 
ACCATACAGG ACACCCTGAA CCCCAAGTGG AACTCCAACT GCCAATTCTT TATCAAGGAC 
CTGGAGCAGG ACGTTCTGTG CATCACCGTG TTTGAGAGGG ACCAGTTTTC CCCTGACGAT 
TTCCTGGGCC GGACAGAAAT CCGAGTTGCT GACATAAAGA AAGACCAAGG TTCTAAAGGA 
CCAGTAACCA AGTGTCTACT TCTGCACGAA GTTCCTACTG GAGAGATAGT GGTCCGATTG 
GATCTGCAGC TGTTTGACGA GCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_004912128.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912185.1 Xenopus tropicalis intersectin 1 (itsn1), transcript variant X3, mRNA.

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CloneID	OXa06416
Clone ID Related Accession (Same CDS sequence)	XM_004912128.3 , XM_004912128.4
Accession Version	XM_004912128.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intersectin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912128.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGCCACGT TGCCAAAGAG TTCTTCATTC GGCCGCTCAG GTGCCGGCTC CCAGATGAAC 
ACTAAACTTC AGAAAGCTCA GTCCTTTGAT GTCCCAACCC CCCCACCCCT GGCAGAATGG 
GCTGTACCAC AGTCTTCAAG GTTGAAGTAC AGACAGTTGT TCAACAGCCA CGACAAAACC 
ATGAGTGGGC ATTTAACAGG TCCTCAAGCA AGAACTATTC TTATGCAGTC AAGTTTACCG 
CAGAGCCAGC TAGCTACGAT ATGGAACCTT TCAGACATTG ACCTAGATGG AAAACTAACG 
GCCGAAGAAT TTATTTTAGC TATGCACTTA ATAGATGTGG CCATGTCTGG CCAGCCACTT 
CCTCCAGTTC TGCCTCCTGA GTATATTCCC CCATCTTTTA GAAGAGTTCG ATCGGGGAGC 
GGGTTATCGA TTATGAGCTC GGTGTCTGTA GATCAGCGCT TACCGGAGGA ACCAGCAGAA 
GAGGAGCCAC AGAACACAGA CAAAAGACTT CCAGTGACAT TTGAAGACAA GAAAAGGGAA 
AACTTTGAGC GAGGAAACCT GGAATTAGAA AAGAGGAGAC AGGCACTTCT AGAGCAGCAG 
CGCAAAGAGC AGGAGAGGCT GGCACAGCTG GAAAGGGCAG AGCAGGAGAG AAAAGAACGA 
GAGCGGCAAG ATCAAGAGCG CAAGCGGCAG CAAGAGCTGG AAAAACAGCT GGAGAAACAA 
CGAGAGCTGG AGCGGCAACG GGAAGAGGAG CGCAGGAAAG AAATAGAAAG GCGAGAGGCA 
GCAAAGAGAG AACTTGAAAG GCAGAGGCAG TTGGAGTGGG AACGAAACCG GAGGCAAGAA 
CTCCTTAATC AAAGGAACAG AGAGCAAGAG GACATTGTTG TCTTAAAAGC AAAGAAAAAG 
ACTCTTGAGT TTGAGCTGGA AGCTCTAAAT GATAAAAAGC ACCAGCTGGA AGGGAAACTT 
CAGGATATCC GCTGTCGCCT CACTACTCAA CGGCACGAAA TAGAAAGCAC TAACAAATCC 
AGGGAGCTTA GGATCGCAGA GATCACGCAT TTACAGCAGC AGCTTCAGGA ATCTCAGCAG 
CTGCTTGGAA AACTGATTCC TGAGAAACAG TCCCTTAACG ATCAATTAAA GCAGGTTCAA 
CAAAACAGTT TGCACAGAGA TTCCTTGCTG ACACTAAAAA GGGCATTGGA AGCCAAGGAG 
ATGGGCCGCC AGCAGCTCCG AGACCAGTTG GATGAAGTAG AAAAAGAAAC ACGAGCTAAG 
CTCCAGGAAA TCGATGTGTT TAATAACCAA TTAAAGGAAC TGAGAGAATT GTACAACAGA 
CAACAGCTCC AGAAACAGAA GGACTTTGAA GCCGAGAAGA TCAAGCAGAA AGAGCTGGAA 
CGGAAAACCA GTGAGCTGGA CAAGATGAAA GAGGAGGACA AAAGGCGGAT GCTAGAGCAG 
GATAAGCTTT GGCAGGATCG TGTGAAGCAG GAAGAGGAAC GTTACAAATT CCAAGATGAG 
GAGAAGCAGA AAAGGGAAGA ATCCGCTCAA CAATGTGAGG AAGAAAAGAA ACCAGAGATC 
CAAGAGAAGC CCAACAAACT GTTCCACCTA CCACCGGAGC CAGGAAAGCT AGGAGGGCAT 
GTCCCCTGGA TGAATACAGA GAAAGCACCG CTAACATTCA ACCAGGGTGA TGTTAAGGTT 
GTGTATTACC GGGCACTCTA CCCTTTTGAT GCTCGAAGTC ATGATGAAAT CACTATTCAG 
CCTGGGGACA TTATTATGGT GGATGAGAGC CAAACCGGAG AGCCTGGATG GCTGGGAGGT 
GAACTGAAGG GAAAAACTGG CTGGTTTCCT GCCAACTATG CAGAGAGAAT GCCCGAGGGT 
GAATTCCCAA GCACAACTAA ACAGGCAGCA GACACTACGG CCAAACCCAC TGTCCATTTG 
GCACCTTCCA CTCCCACACC TGCAGCTTTT ACAGATAACT CTGCAAATTC CAACAACTGG 
GCAGACTTCA GCTCCACGTG GCCAACCAAT AACACTGATA AAGCAGAGAC GGATAACTGG 
GATACATGGG GAGCTCAACC ATCTCTGACT GTTCCTAGTG CCGGACAGCA CAGGCAGCGC 
TCTGCTTTTA CCCCAGCAAC GGTTACTGGA TCATCTCCAT CTCCTGTACT TGGTCAGGGT 
GAAAAAGTAG AAGGGCTTCA GGCACAAGCC TTGTATCCTT GGAGAGCAAA AAAGGACAAC 
CACCTTAATT TTAACAAAAA TGACATCATC ACGGTTCTAG AGCAGCAGGA TATGTGGTGG 
TTTGGGGAAG TTCAAGGTCA AAAGGGCTGG TTTCCCAAAT CCTACGTAAA ACTTATATCC 
GGGCCACTAA GGAAATCTAC AAGCATCGAT TCCACATCTT CAGAAAGCCC TGCTAGTTTA 
AAAAGAGTCT CTTCACCAGC ATTTAAACCT GCCATGCAAG GAGAAGAATA CATAGCCATG 
TACACGTACG AGAGCAACGA GCAGGGAGAC TTGACCTTTC AGCAAGGAGA TCTGATTGTG 
GTGATTAAAA AAGATGGGGA CTGGTGGACT GGCACGGTGG GAGAGAAGAC GGGGGTTTTC 
CCTTCCAACT ACGTAAGGCC CAAAGACTCC GAGGCGGCTG GAAGCGCAGG AAAGACAGGA 
AGCCTTGGGA AGAAACCAGA AATTGCCCAG GTTATTGCTT CTTACACAGC AACTGGCCCT 
GAGCAGCTGA CACTGGCTCC TGGCCAACTT ATCCTCATCC GGAAAAAGAA TCCTGGAGGA 
TGGTGGGAAG GAGAACTGCA GGCACGTGGG AAGAAGCGAC AGATTGGATG GTTTCCAGCA 
AATTATGTGA AGCTTTTGAG CCCTGGTACA AACAAAAGCA CGCCGACAGA ACCACCAAAA 
GCAACATCAT TGCCTCCAAC ATGCCAAGTC ATCGGGATGT ACGATTATAT CGCTCAGAAT 
GACGATGAGT TGGCCTTCGG TAAAGGTCAG GTCATTAACG TGCTGAACAA AGAGGACCCG 
GACTGGTGGA AAGGAGAACT GAACGGCCAT GTTGGTCTGT TTCCCTCCAA CTATGTGAAA 
CTGACCACAG ACATGGATCC CAGTCAGCAA TGGTGTGCAG ACCTACATCT CCTGGACATG 
CTGAGTCCCA CCGAGAGGAA GAGGCAAGGG TACATCCACG AGCTCATTGT CACCGAGGAG 
AACTATGTCA ATGACTTACA GTTAGTCACC GAGACATTCC AAAAGCCTCT GCTGGAATCT 
GAATTACTGA CGGAGAAGGA AGTTGCCATG ATTTTTGTCA ACTGGAAGGA GCTGATAATG 
TGCAATATCA AACTGCTCAA GGCCTTGCGA GTTCGCAAGA AAATGTCTGG GGAGAAGATG 
CCGGTGAAGA TGATCGGGGA CATCCTGACG GCCCAGTTGC CCCACATGCA GCCGTACATT 
CGCTTCTGCA GCTGCCAGCT CAACGGCGCC GCTCTCATTC AGCAGAAAAC GGACGAGGTG 
CCCGAGTTCA AGGAGTTTGT GAAAAGGCTT GCAATGGATC CCCGGTGTAA GGGAATGCCG 
CTGTCCAGCT TCCTACTCAA ACCCATGCAG AGAGTAACCA GATACCCGCT CATCATTAAA 
AATATTATTG AAAATACTCC AGAAAATCAC CCTGACCACA GCCACCTGAA GCAAGCCCTA 
GAGAAAGCCG AAGAATTATG TTCCCAAGTG AATGAGGGGG TTCGGGAGAA GGAAAACTCA 
GACCGACTGG AGTGGATTCA GGCGCACGTT CAGTGTGAGG GTTTGTCTGA GCAATTAGTA 
TTTAACTCTG TTACAAACTG CCTGGGCCCA CGCAAGTTCC TGCACAGTGG GAAACTGTAC 
AAAGCCAAGA GCAACAAGGA ACTATACGGC TTCCTGTTCA ACGACTTCCT GCTGCTCACC 
CAGATCATTA AGCCCCTCGG CTCCTCCGGC AACGATAAGG TGTTCAGCCC CAAGTCCAAT 
CTCCAGTACA AGATGTACAA AACTCCAATA TTCCTCAACG AAGTGCTGGT GAAACTGCCC 
ACGGACCCAT CCGGGGATGA GCCAATCTTT CATATTTCTC ACATTGACCG AGTCTACACA 
TTGCGTGCAG AGAGCATCAA TGAGAGGACG GCCTGGGTGC AGAAAATCAA AGCGGCATCG 
GAGTTGTACA TAGAGACGGA GAAGAAGAAG AGAGAGAAGG CCTACTTGGT TCGCTCCCAA 
CGCGCAACAG GCATCGGAAG ACTGATGGTA AATATCGTTG AGGGAATTGA GTTGAAGCCC 
TGCAGAACCC ACGGGAAGAG CAATCCTTAC TGTGAGATCA CCATGGGCTC CCAGTGCCAC 
ATTACCAAGA CCATACAGGA CACCCTGAAC CCCAAGTGGA ACTCCAACTG CCAATTCTTT 
ATCAAGGACC TGGAGCAGGA CGTTCTGTGC ATCACCGTGT TTGAGAGGGA CCAGTTTTCC 
CCTGACGATT TCCTGGGCCG GACAGAAATC CGAGTTGCTG ACATAAAGAA AGACCAAGGT 
TCTAAAGGAC CAGTAACCAA GTGTCTACTT CTGCACGAAG TTCCTACTGG AGAGATAGTG 
GTCCGATTGG ATCTGCAGCT GTTTGACGAG CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912185.1
CDS	267..5222
Translation	MAQFGTPFGGNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSAAAAAAAAAGFATLPKSSSFGRSGAGSQMNTKLQKAQSFDVPTPPPLAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGLSIMSSVSVDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRELRIAEITHLQQQLQESQQLLGKLIPEKQSLNDQLKQVQQNSLHRDSLLTLKRALEAKEMGRQQLRDQLDEVEKETRAKLQEIDVFNNQLKELRELYNRQQLQKQKDFEAEKIKQKELERKTSELDKMKEEDKRRMLEQDKLWQDRVKQEEERYKFQDEEKQKREESAQQCEEEKKPEIQEKPNKLFHLPPEPGKLGGHVPWMNTEKAPLTFNQGDVKVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEPGWLGGELKGKTGWFPANYAERMPEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTWPTNNTDKAETDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVTGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRVSSPAFKPAMQGEEYIAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTNKSTPTEPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGELNGHVGLFPSNYVKLTTDMDPSQQWCADLHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_004912128.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912128.4

ORF Insert Sequence:

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGCCACGT TGCCAAAGAG TTCTTCATTC GGCCGCTCAG GTGCCGGCTC CCAGATGAAC 
ACTAAACTTC AGAAAGCTCA GTCCTTTGAT GTCCCAACCC CCCCACCCCT GGCAGAATGG 
GCTGTACCAC AGTCTTCAAG GTTGAAGTAC AGACAGTTGT TCAACAGCCA CGACAAAACC 
ATGAGTGGGC ATTTAACAGG TCCTCAAGCA AGAACTATTC TTATGCAGTC AAGTTTACCG 
CAGAGCCAGC TAGCTACGAT ATGGAACCTT TCAGACATTG ACCTAGATGG AAAACTAACG 
GCCGAAGAAT TTATTTTAGC TATGCACTTA ATAGATGTGG CCATGTCTGG CCAGCCACTT 
CCTCCAGTTC TGCCTCCTGA GTATATTCCC CCATCTTTTA GAAGAGTTCG ATCGGGGAGC 
GGGTTATCGA TTATGAGCTC GGTGTCTGTA GATCAGCGCT TACCGGAGGA ACCAGCAGAA 
GAGGAGCCAC AGAACACAGA CAAAAGACTT CCAGTGACAT TTGAAGACAA GAAAAGGGAA 
AACTTTGAGC GAGGAAACCT GGAATTAGAA AAGAGGAGAC AGGCACTTCT AGAGCAGCAG 
CGCAAAGAGC AGGAGAGGCT GGCACAGCTG GAAAGGGCAG AGCAGGAGAG AAAAGAACGA 
GAGCGGCAAG ATCAAGAGCG CAAGCGGCAG CAAGAGCTGG AAAAACAGCT GGAGAAACAA 
CGAGAGCTGG AGCGGCAACG GGAAGAGGAG CGCAGGAAAG AAATAGAAAG GCGAGAGGCA 
GCAAAGAGAG AACTTGAAAG GCAGAGGCAG TTGGAGTGGG AACGAAACCG GAGGCAAGAA 
CTCCTTAATC AAAGGAACAG AGAGCAAGAG GACATTGTTG TCTTAAAAGC AAAGAAAAAG 
ACTCTTGAGT TTGAGCTGGA AGCTCTAAAT GATAAAAAGC ACCAGCTGGA AGGGAAACTT 
CAGGATATCC GCTGTCGCCT CACTACTCAA CGGCACGAAA TAGAAAGCAC TAACAAATCC 
AGGGAGCTTA GGATCGCAGA GATCACGCAT TTACAGCAGC AGCTTCAGGA ATCTCAGCAG 
CTGCTTGGAA AACTGATTCC TGAGAAACAG TCCCTTAACG ATCAATTAAA GCAGGTTCAA 
CAAAACAGTT TGCACAGAGA TTCCTTGCTG ACACTAAAAA GGGCATTGGA AGCCAAGGAG 
ATGGGCCGCC AGCAGCTCCG AGACCAGTTG GATGAAGTAG AAAAAGAAAC ACGAGCTAAG 
CTCCAGGAAA TCGATGTGTT TAATAACCAA TTAAAGGAAC TGAGAGAATT GTACAACAGA 
CAACAGCTCC AGAAACAGAA GGACTTTGAA GCCGAGAAGA TCAAGCAGAA AGAGCTGGAA 
CGGAAAACCA GTGAGCTGGA CAAGATGAAA GAGGAGGACA AAAGGCGGAT GCTAGAGCAG 
GATAAGCTTT GGCAGGATCG TGTGAAGCAG GAAGAGGAAC GTTACAAATT CCAAGATGAG 
GAGAAGCAGA AAAGGGAAGA ATCCGCTCAA CAATGTGAGG AAGAAAAGAA ACCAGAGATC 
CAAGAGAAGC CCAACAAACT GTTCCACCTA CCACCGGAGC CAGGAAAGCT AGGAGGGCAT 
GTCCCCTGGA TGAATACAGA GAAAGCACCG CTAACATTCA ACCAGGGTGA TGTTAAGGTT 
GTGTATTACC GGGCACTCTA CCCTTTTGAT GCTCGAAGTC ATGATGAAAT CACTATTCAG 
CCTGGGGACA TTATTATGGT GGATGAGAGC CAAACCGGAG AGCCTGGATG GCTGGGAGGT 
GAACTGAAGG GAAAAACTGG CTGGTTTCCT GCCAACTATG CAGAGAGAAT GCCCGAGGGT 
GAATTCCCAA GCACAACTAA ACAGGCAGCA GACACTACGG CCAAACCCAC TGTCCATTTG 
GCACCTTCCA CTCCCACACC TGCAGCTTTT ACAGATAACT CTGCAAATTC CAACAACTGG 
GCAGACTTCA GCTCCACGTG GCCAACCAAT AACACTGATA AAGCAGAGAC GGATAACTGG 
GATACATGGG GAGCTCAACC ATCTCTGACT GTTCCTAGTG CCGGACAGCA CAGGCAGCGC 
TCTGCTTTTA CCCCAGCAAC GGTTACTGGA TCATCTCCAT CTCCTGTACT TGGTCAGGGT 
GAAAAAGTAG AAGGGCTTCA GGCACAAGCC TTGTATCCTT GGAGAGCAAA AAAGGACAAC 
CACCTTAATT TTAACAAAAA TGACATCATC ACGGTTCTAG AGCAGCAGGA TATGTGGTGG 
TTTGGGGAAG TTCAAGGTCA AAAGGGCTGG TTTCCCAAAT CCTACGTAAA ACTTATATCC 
GGGCCACTAA GGAAATCTAC AAGCATCGAT TCCACATCTT CAGAAAGCCC TGCTAGTTTA 
AAAAGAGTCT CTTCACCAGC ATTTAAACCT GCCATGCAAG GAGAAGAATA CATAGCCATG 
TACACGTACG AGAGCAACGA GCAGGGAGAC TTGACCTTTC AGCAAGGAGA TCTGATTGTG 
GTGATTAAAA AAGATGGGGA CTGGTGGACT GGCACGGTGG GAGAGAAGAC GGGGGTTTTC 
CCTTCCAACT ACGTAAGGCC CAAAGACTCC GAGGCGGCTG GAAGCGCAGG AAAGACAGGA 
AGCCTTGGGA AGAAACCAGA AATTGCCCAG GTTATTGCTT CTTACACAGC AACTGGCCCT 
GAGCAGCTGA CACTGGCTCC TGGCCAACTT ATCCTCATCC GGAAAAAGAA TCCTGGAGGA 
TGGTGGGAAG GAGAACTGCA GGCACGTGGG AAGAAGCGAC AGATTGGATG GTTTCCAGCA 
AATTATGTGA AGCTTTTGAG CCCTGGTACA AACAAAAGCA CGCCGACAGA ACCACCAAAA 
GCAACATCAT TGCCTCCAAC ATGCCAAGTC ATCGGGATGT ACGATTATAT CGCTCAGAAT 
GACGATGAGT TGGCCTTCGG TAAAGGTCAG GTCATTAACG TGCTGAACAA AGAGGACCCG 
GACTGGTGGA AAGGAGAACT GAACGGCCAT GTTGGTCTGT TTCCCTCCAA CTATGTGAAA 
CTGACCACAG ACATGGATCC CAGTCAGCAA TGGTGTGCAG ACCTACATCT CCTGGACATG 
CTGAGTCCCA CCGAGAGGAA GAGGCAAGGG TACATCCACG AGCTCATTGT CACCGAGGAG 
AACTATGTCA ATGACTTACA GTTAGTCACC GAGACATTCC AAAAGCCTCT GCTGGAATCT 
GAATTACTGA CGGAGAAGGA AGTTGCCATG ATTTTTGTCA ACTGGAAGGA GCTGATAATG 
TGCAATATCA AACTGCTCAA GGCCTTGCGA GTTCGCAAGA AAATGTCTGG GGAGAAGATG 
CCGGTGAAGA TGATCGGGGA CATCCTGACG GCCCAGTTGC CCCACATGCA GCCGTACATT 
CGCTTCTGCA GCTGCCAGCT CAACGGCGCC GCTCTCATTC AGCAGAAAAC GGACGAGGTG 
CCCGAGTTCA AGGAGTTTGT GAAAAGGCTT GCAATGGATC CCCGGTGTAA GGGAATGCCG 
CTGTCCAGCT TCCTACTCAA ACCCATGCAG AGAGTAACCA GATACCCGCT CATCATTAAA 
AATATTATTG AAAATACTCC AGAAAATCAC CCTGACCACA GCCACCTGAA GCAAGCCCTA 
GAGAAAGCCG AAGAATTATG TTCCCAAGTG AATGAGGGGG TTCGGGAGAA GGAAAACTCA 
GACCGACTGG AGTGGATTCA GGCGCACGTT CAGTGTGAGG GTTTGTCTGA GCAATTAGTA 
TTTAACTCTG TTACAAACTG CCTGGGCCCA CGCAAGTTCC TGCACAGTGG GAAACTGTAC 
AAAGCCAAGA GCAACAAGGA ACTATACGGC TTCCTGTTCA ACGACTTCCT GCTGCTCACC 
CAGATCATTA AGCCCCTCGG CTCCTCCGGC AACGATAAGG TGTTCAGCCC CAAGTCCAAT 
CTCCAGTACA AGATGTACAA AACTCCAATA TTCCTCAACG AAGTGCTGGT GAAACTGCCC 
ACGGACCCAT CCGGGGATGA GCCAATCTTT CATATTTCTC ACATTGACCG AGTCTACACA 
TTGCGTGCAG AGAGCATCAA TGAGAGGACG GCCTGGGTGC AGAAAATCAA AGCGGCATCG 
GAGTTGTACA TAGAGACGGA GAAGAAGAAG AGAGAGAAGG CCTACTTGGT TCGCTCCCAA 
CGCGCAACAG GCATCGGAAG ACTGATGGTA AATATCGTTG AGGGAATTGA GTTGAAGCCC 
TGCAGAACCC ACGGGAAGAG CAATCCTTAC TGTGAGATCA CCATGGGCTC CCAGTGCCAC 
ATTACCAAGA CCATACAGGA CACCCTGAAC CCCAAGTGGA ACTCCAACTG CCAATTCTTT 
ATCAAGGACC TGGAGCAGGA CGTTCTGTGC ATCACCGTGT TTGAGAGGGA CCAGTTTTCC 
CCTGACGATT TCCTGGGCCG GACAGAAATC CGAGTTGCTG ACATAAAGAA AGACCAAGGT 
TCTAAAGGAC CAGTAACCAA GTGTCTACTT CTGCACGAAG TTCCTACTGG AGAGATAGTG 
GTCCGATTGG ATCTGCAGCT GTTTGACGAG CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_004912129.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912186.1 Xenopus tropicalis intersectin 1 (itsn1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa06417
Clone ID Related Accession (Same CDS sequence)	XM_004912129.3 , XM_004912129.4
Accession Version	XM_004912129.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intersectin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912129.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGCCACGT TGCCAAAGAG TTCTTCATTC GGCCGCTCAG GTGCCGGCTC CCAGATGAAC 
ACTAAACTTC AGAAAGCTCA GTCCTTTGAT GTCCCAACCC CCCCACCCCT GGCAGAATGG 
GCTGTACCAC AGTCTTCAAG GTTGAAGTAC AGACAGTTGT TCAACAGCCA CGACAAAACC 
ATGAGTGGGC ATTTAACAGG TCCTCAAGCA AGAACTATTC TTATGCAGTC AAGTTTACCG 
CAGAGCCAGC TAGCTACGAT ATGGAACCTT TCAGACATTG ACCTAGATGG AAAACTAACG 
GCCGAAGAAT TTATTTTAGC TATGCACTTA ATAGATGTGG CCATGTCTGG CCAGCCACTT 
CCTCCAGTTC TGCCTCCTGA GTATATTCCC CCATCTTTTA GAGTTCGATC GGGGAGCGGG 
TTATCGATTA TGAGCTCGGT GTCTGTAGAT CAGCGCTTAC CGGAGGAACC AGCAGAAGAG 
GAGCCACAGA ACACAGACAA AAGACTTCCA GTGACATTTG AAGACAAGAA AAGGGAAAAC 
TTTGAGCGAG GAAACCTGGA ATTAGAAAAG AGGAGACAGG CACTTCTAGA GCAGCAGCGC 
AAAGAGCAGG AGAGGCTGGC ACAGCTGGAA AGGGCAGAGC AGGAGAGAAA AGAACGAGAG 
CGGCAAGATC AAGAGCGCAA GCGGCAGCAA GAGCTGGAAA AACAGCTGGA GAAACAACGA 
GAGCTGGAGC GGCAACGGGA AGAGGAGCGC AGGAAAGAAA TAGAAAGGCG AGAGGCAGCA 
AAGAGAGAAC TTGAAAGGCA GAGGCAGTTG GAGTGGGAAC GAAACCGGAG GCAAGAACTC 
CTTAATCAAA GGAACAGAGA GCAAGAGGAC ATTGTTGTCT TAAAAGCAAA GAAAAAGACT 
CTTGAGTTTG AGCTGGAAGC TCTAAATGAT AAAAAGCACC AGCTGGAAGG GAAACTTCAG 
GATATCCGCT GTCGCCTCAC TACTCAACGG CACGAAATAG AAAGCACTAA CAAATCCAGG 
GAGCTTAGGA TCGCAGAGAT CACGCATTTA CAGCAGCAGC TTCAGGAATC TCAGCAGCTG 
CTTGGAAAAC TGATTCCTGA GAAACAGTCC CTTAACGATC AATTAAAGCA GGTTCAACAA 
AACAGTTTGC ACAGAGATTC CTTGCTGACA CTAAAAAGGG CATTGGAAGC CAAGGAGATG 
GGCCGCCAGC AGCTCCGAGA CCAGTTGGAT GAAGTAGAAA AAGAAACACG AGCTAAGCTC 
CAGGAAATCG ATGTGTTTAA TAACCAATTA AAGGAACTGA GAGAATTGTA CAACAGACAA 
CAGCTCCAGA AACAGAAGGA CTTTGAAGCC GAGAAGATCA AGCAGAAAGA GCTGGAACGG 
AAAACCAGTG AGCTGGACAA GATGAAAGAG GAGGACAAAA GGCGGATGCT AGAGCAGGAT 
AAGCTTTGGC AGGATCGTGT GAAGCAGGAA GAGGAACGTT ACAAATTCCA AGATGAGGAG 
AAGCAGAAAA GGGAAGAATC CGCTCAACAA TGTGAGGAAG AAAAGAAACC AGAGATCCAA 
GAGAAGCCCA ACAAACTGTT CCACCTACCA CCGGAGCCAG GAAAGCTAGG AGGGCATGTC 
CCCTGGATGA ATACAGAGAA AGCACCGCTA ACATTCAACC AGGGTGATGT TAAGGTTGTG 
TATTACCGGG CACTCTACCC TTTTGATGCT CGAAGTCATG ATGAAATCAC TATTCAGCCT 
GGGGACATTA TTATGGTGGA TGAGAGCCAA ACCGGAGAGC CTGGATGGCT GGGAGGTGAA 
CTGAAGGGAA AAACTGGCTG GTTTCCTGCC AACTATGCAG AGAGAATGCC CGAGGGTGAA 
TTCCCAAGCA CAACTAAACA GGCAGCAGAC ACTACGGCCA AACCCACTGT CCATTTGGCA 
CCTTCCACTC CCACACCTGC AGCTTTTACA GATAACTCTG CAAATTCCAA CAACTGGGCA 
GACTTCAGCT CCACGTGGCC AACCAATAAC ACTGATAAAG CAGAGACGGA TAACTGGGAT 
ACATGGGGAG CTCAACCATC TCTGACTGTT CCTAGTGCCG GACAGCACAG GCAGCGCTCT 
GCTTTTACCC CAGCAACGGT TACTGGATCA TCTCCATCTC CTGTACTTGG TCAGGGTGAA 
AAAGTAGAAG GGCTTCAGGC ACAAGCCTTG TATCCTTGGA GAGCAAAAAA GGACAACCAC 
CTTAATTTTA ACAAAAATGA CATCATCACG GTTCTAGAGC AGCAGGATAT GTGGTGGTTT 
GGGGAAGTTC AAGGTCAAAA GGGCTGGTTT CCCAAATCCT ACGTAAAACT TATATCCGGG 
CCACTAAGGA AATCTACAAG CATCGATTCC ACATCTTCAG AAAGCCCTGC TAGTTTAAAA 
AGAGTCTCTT CACCAGCATT TAAACCTGCC ATGCAAGGAG AAGAATACAT AGCCATGTAC 
ACGTACGAGA GCAACGAGCA GGGAGACTTG ACCTTTCAGC AAGGAGATCT GATTGTGGTG 
ATTAAAAAAG ATGGGGACTG GTGGACTGGC ACGGTGGGAG AGAAGACGGG GGTTTTCCCT 
TCCAACTACG TAAGGCCCAA AGACTCCGAG GCGGCTGGAA GCGCAGGAAA GACAGGAAGC 
CTTGGGAAGA AACCAGAAAT TGCCCAGGTT ATTGCTTCTT ACACAGCAAC TGGCCCTGAG 
CAGCTGACAC TGGCTCCTGG CCAACTTATC CTCATCCGGA AAAAGAATCC TGGAGGATGG 
TGGGAAGGAG AACTGCAGGC ACGTGGGAAG AAGCGACAGA TTGGATGGTT TCCAGCAAAT 
TATGTGAAGC TTTTGAGCCC TGGTACAAAC AAAAGCACGC CGACAGAACC ACCAAAAGCA 
ACATCATTGC CTCCAACATG CCAAGTCATC GGGATGTACG ATTATATCGC TCAGAATGAC 
GATGAGTTGG CCTTCGGTAA AGGTCAGGTC ATTAACGTGC TGAACAAAGA GGACCCGGAC 
TGGTGGAAAG GAGAACTGAA CGGCCATGTT GGTCTGTTTC CCTCCAACTA TGTGAAACTG 
ACCACAGACA TGGATCCCAG TCAGCAATGG TGTGCAGACC TACATCTCCT GGACATGCTG 
AGTCCCACCG AGAGGAAGAG GCAAGGGTAC ATCCACGAGC TCATTGTCAC CGAGGAGAAC 
TATGTCAATG ACTTACAGTT AGTCACCGAG ACATTCCAAA AGCCTCTGCT GGAATCTGAA 
TTACTGACGG AGAAGGAAGT TGCCATGATT TTTGTCAACT GGAAGGAGCT GATAATGTGC 
AATATCAAAC TGCTCAAGGC CTTGCGAGTT CGCAAGAAAA TGTCTGGGGA GAAGATGCCG 
GTGAAGATGA TCGGGGACAT CCTGACGGCC CAGTTGCCCC ACATGCAGCC GTACATTCGC 
TTCTGCAGCT GCCAGCTCAA CGGCGCCGCT CTCATTCAGC AGAAAACGGA CGAGGTGCCC 
GAGTTCAAGG AGTTTGTGAA AAGGCTTGCA ATGGATCCCC GGTGTAAGGG AATGCCGCTG 
TCCAGCTTCC TACTCAAACC CATGCAGAGA GTAACCAGAT ACCCGCTCAT CATTAAAAAT 
ATTATTGAAA ATACTCCAGA AAATCACCCT GACCACAGCC ACCTGAAGCA AGCCCTAGAG 
AAAGCCGAAG AATTATGTTC CCAAGTGAAT GAGGGGGTTC GGGAGAAGGA AAACTCAGAC 
CGACTGGAGT GGATTCAGGC GCACGTTCAG TGTGAGGGTT TGTCTGAGCA ATTAGTATTT 
AACTCTGTTA CAAACTGCCT GGGCCCACGC AAGTTCCTGC ACAGTGGGAA ACTGTACAAA 
GCCAAGAGCA ACAAGGAACT ATACGGCTTC CTGTTCAACG ACTTCCTGCT GCTCACCCAG 
ATCATTAAGC CCCTCGGCTC CTCCGGCAAC GATAAGGTGT TCAGCCCCAA GTCCAATCTC 
CAGTACAAGA TGTACAAAAC TCCAATATTC CTCAACGAAG TGCTGGTGAA ACTGCCCACG 
GACCCATCCG GGGATGAGCC AATCTTTCAT ATTTCTCACA TTGACCGAGT CTACACATTG 
CGTGCAGAGA GCATCAATGA GAGGACGGCC TGGGTGCAGA AAATCAAAGC GGCATCGGAG 
TTGTACATAG AGACGGAGAA GAAGAAGAGA GAGAAGGCCT ACTTGGTTCG CTCCCAACGC 
GCAACAGGCA TCGGAAGACT GATGGTAAAT ATCGTTGAGG GAATTGAGTT GAAGCCCTGC 
AGAACCCACG GGAAGAGCAA TCCTTACTGT GAGATCACCA TGGGCTCCCA GTGCCACATT 
ACCAAGACCA TACAGGACAC CCTGAACCCC AAGTGGAACT CCAACTGCCA ATTCTTTATC 
AAGGACCTGG AGCAGGACGT TCTGTGCATC ACCGTGTTTG AGAGGGACCA GTTTTCCCCT 
GACGATTTCC TGGGCCGGAC AGAAATCCGA GTTGCTGACA TAAAGAAAGA CCAAGGTTCT 
AAAGGACCAG TAACCAAGTG TCTACTTCTG CACGAAGTTC CTACTGGAGA GATAGTGGTC 
CGATTGGATC TGCAGCTGTT TGACGAGCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912186.1
CDS	266..5218
Translation	MAQFGTPFGGNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSAAAAAAAAAGFATLPKSSSFGRSGAGSQMNTKLQKAQSFDVPTPPPLAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRVRSGSGLSIMSSVSVDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRELRIAEITHLQQQLQESQQLLGKLIPEKQSLNDQLKQVQQNSLHRDSLLTLKRALEAKEMGRQQLRDQLDEVEKETRAKLQEIDVFNNQLKELRELYNRQQLQKQKDFEAEKIKQKELERKTSELDKMKEEDKRRMLEQDKLWQDRVKQEEERYKFQDEEKQKREESAQQCEEEKKPEIQEKPNKLFHLPPEPGKLGGHVPWMNTEKAPLTFNQGDVKVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEPGWLGGELKGKTGWFPANYAERMPEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTWPTNNTDKAETDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVTGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRVSSPAFKPAMQGEEYIAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTNKSTPTEPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGELNGHVGLFPSNYVKLTTDMDPSQQWCADLHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_004912129.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912129.4

ORF Insert Sequence:

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGCCACGT TGCCAAAGAG TTCTTCATTC GGCCGCTCAG GTGCCGGCTC CCAGATGAAC 
ACTAAACTTC AGAAAGCTCA GTCCTTTGAT GTCCCAACCC CCCCACCCCT GGCAGAATGG 
GCTGTACCAC AGTCTTCAAG GTTGAAGTAC AGACAGTTGT TCAACAGCCA CGACAAAACC 
ATGAGTGGGC ATTTAACAGG TCCTCAAGCA AGAACTATTC TTATGCAGTC AAGTTTACCG 
CAGAGCCAGC TAGCTACGAT ATGGAACCTT TCAGACATTG ACCTAGATGG AAAACTAACG 
GCCGAAGAAT TTATTTTAGC TATGCACTTA ATAGATGTGG CCATGTCTGG CCAGCCACTT 
CCTCCAGTTC TGCCTCCTGA GTATATTCCC CCATCTTTTA GAGTTCGATC GGGGAGCGGG 
TTATCGATTA TGAGCTCGGT GTCTGTAGAT CAGCGCTTAC CGGAGGAACC AGCAGAAGAG 
GAGCCACAGA ACACAGACAA AAGACTTCCA GTGACATTTG AAGACAAGAA AAGGGAAAAC 
TTTGAGCGAG GAAACCTGGA ATTAGAAAAG AGGAGACAGG CACTTCTAGA GCAGCAGCGC 
AAAGAGCAGG AGAGGCTGGC ACAGCTGGAA AGGGCAGAGC AGGAGAGAAA AGAACGAGAG 
CGGCAAGATC AAGAGCGCAA GCGGCAGCAA GAGCTGGAAA AACAGCTGGA GAAACAACGA 
GAGCTGGAGC GGCAACGGGA AGAGGAGCGC AGGAAAGAAA TAGAAAGGCG AGAGGCAGCA 
AAGAGAGAAC TTGAAAGGCA GAGGCAGTTG GAGTGGGAAC GAAACCGGAG GCAAGAACTC 
CTTAATCAAA GGAACAGAGA GCAAGAGGAC ATTGTTGTCT TAAAAGCAAA GAAAAAGACT 
CTTGAGTTTG AGCTGGAAGC TCTAAATGAT AAAAAGCACC AGCTGGAAGG GAAACTTCAG 
GATATCCGCT GTCGCCTCAC TACTCAACGG CACGAAATAG AAAGCACTAA CAAATCCAGG 
GAGCTTAGGA TCGCAGAGAT CACGCATTTA CAGCAGCAGC TTCAGGAATC TCAGCAGCTG 
CTTGGAAAAC TGATTCCTGA GAAACAGTCC CTTAACGATC AATTAAAGCA GGTTCAACAA 
AACAGTTTGC ACAGAGATTC CTTGCTGACA CTAAAAAGGG CATTGGAAGC CAAGGAGATG 
GGCCGCCAGC AGCTCCGAGA CCAGTTGGAT GAAGTAGAAA AAGAAACACG AGCTAAGCTC 
CAGGAAATCG ATGTGTTTAA TAACCAATTA AAGGAACTGA GAGAATTGTA CAACAGACAA 
CAGCTCCAGA AACAGAAGGA CTTTGAAGCC GAGAAGATCA AGCAGAAAGA GCTGGAACGG 
AAAACCAGTG AGCTGGACAA GATGAAAGAG GAGGACAAAA GGCGGATGCT AGAGCAGGAT 
AAGCTTTGGC AGGATCGTGT GAAGCAGGAA GAGGAACGTT ACAAATTCCA AGATGAGGAG 
AAGCAGAAAA GGGAAGAATC CGCTCAACAA TGTGAGGAAG AAAAGAAACC AGAGATCCAA 
GAGAAGCCCA ACAAACTGTT CCACCTACCA CCGGAGCCAG GAAAGCTAGG AGGGCATGTC 
CCCTGGATGA ATACAGAGAA AGCACCGCTA ACATTCAACC AGGGTGATGT TAAGGTTGTG 
TATTACCGGG CACTCTACCC TTTTGATGCT CGAAGTCATG ATGAAATCAC TATTCAGCCT 
GGGGACATTA TTATGGTGGA TGAGAGCCAA ACCGGAGAGC CTGGATGGCT GGGAGGTGAA 
CTGAAGGGAA AAACTGGCTG GTTTCCTGCC AACTATGCAG AGAGAATGCC CGAGGGTGAA 
TTCCCAAGCA CAACTAAACA GGCAGCAGAC ACTACGGCCA AACCCACTGT CCATTTGGCA 
CCTTCCACTC CCACACCTGC AGCTTTTACA GATAACTCTG CAAATTCCAA CAACTGGGCA 
GACTTCAGCT CCACGTGGCC AACCAATAAC ACTGATAAAG CAGAGACGGA TAACTGGGAT 
ACATGGGGAG CTCAACCATC TCTGACTGTT CCTAGTGCCG GACAGCACAG GCAGCGCTCT 
GCTTTTACCC CAGCAACGGT TACTGGATCA TCTCCATCTC CTGTACTTGG TCAGGGTGAA 
AAAGTAGAAG GGCTTCAGGC ACAAGCCTTG TATCCTTGGA GAGCAAAAAA GGACAACCAC 
CTTAATTTTA ACAAAAATGA CATCATCACG GTTCTAGAGC AGCAGGATAT GTGGTGGTTT 
GGGGAAGTTC AAGGTCAAAA GGGCTGGTTT CCCAAATCCT ACGTAAAACT TATATCCGGG 
CCACTAAGGA AATCTACAAG CATCGATTCC ACATCTTCAG AAAGCCCTGC TAGTTTAAAA 
AGAGTCTCTT CACCAGCATT TAAACCTGCC ATGCAAGGAG AAGAATACAT AGCCATGTAC 
ACGTACGAGA GCAACGAGCA GGGAGACTTG ACCTTTCAGC AAGGAGATCT GATTGTGGTG 
ATTAAAAAAG ATGGGGACTG GTGGACTGGC ACGGTGGGAG AGAAGACGGG GGTTTTCCCT 
TCCAACTACG TAAGGCCCAA AGACTCCGAG GCGGCTGGAA GCGCAGGAAA GACAGGAAGC 
CTTGGGAAGA AACCAGAAAT TGCCCAGGTT ATTGCTTCTT ACACAGCAAC TGGCCCTGAG 
CAGCTGACAC TGGCTCCTGG CCAACTTATC CTCATCCGGA AAAAGAATCC TGGAGGATGG 
TGGGAAGGAG AACTGCAGGC ACGTGGGAAG AAGCGACAGA TTGGATGGTT TCCAGCAAAT 
TATGTGAAGC TTTTGAGCCC TGGTACAAAC AAAAGCACGC CGACAGAACC ACCAAAAGCA 
ACATCATTGC CTCCAACATG CCAAGTCATC GGGATGTACG ATTATATCGC TCAGAATGAC 
GATGAGTTGG CCTTCGGTAA AGGTCAGGTC ATTAACGTGC TGAACAAAGA GGACCCGGAC 
TGGTGGAAAG GAGAACTGAA CGGCCATGTT GGTCTGTTTC CCTCCAACTA TGTGAAACTG 
ACCACAGACA TGGATCCCAG TCAGCAATGG TGTGCAGACC TACATCTCCT GGACATGCTG 
AGTCCCACCG AGAGGAAGAG GCAAGGGTAC ATCCACGAGC TCATTGTCAC CGAGGAGAAC 
TATGTCAATG ACTTACAGTT AGTCACCGAG ACATTCCAAA AGCCTCTGCT GGAATCTGAA 
TTACTGACGG AGAAGGAAGT TGCCATGATT TTTGTCAACT GGAAGGAGCT GATAATGTGC 
AATATCAAAC TGCTCAAGGC CTTGCGAGTT CGCAAGAAAA TGTCTGGGGA GAAGATGCCG 
GTGAAGATGA TCGGGGACAT CCTGACGGCC CAGTTGCCCC ACATGCAGCC GTACATTCGC 
TTCTGCAGCT GCCAGCTCAA CGGCGCCGCT CTCATTCAGC AGAAAACGGA CGAGGTGCCC 
GAGTTCAAGG AGTTTGTGAA AAGGCTTGCA ATGGATCCCC GGTGTAAGGG AATGCCGCTG 
TCCAGCTTCC TACTCAAACC CATGCAGAGA GTAACCAGAT ACCCGCTCAT CATTAAAAAT 
ATTATTGAAA ATACTCCAGA AAATCACCCT GACCACAGCC ACCTGAAGCA AGCCCTAGAG 
AAAGCCGAAG AATTATGTTC CCAAGTGAAT GAGGGGGTTC GGGAGAAGGA AAACTCAGAC 
CGACTGGAGT GGATTCAGGC GCACGTTCAG TGTGAGGGTT TGTCTGAGCA ATTAGTATTT 
AACTCTGTTA CAAACTGCCT GGGCCCACGC AAGTTCCTGC ACAGTGGGAA ACTGTACAAA 
GCCAAGAGCA ACAAGGAACT ATACGGCTTC CTGTTCAACG ACTTCCTGCT GCTCACCCAG 
ATCATTAAGC CCCTCGGCTC CTCCGGCAAC GATAAGGTGT TCAGCCCCAA GTCCAATCTC 
CAGTACAAGA TGTACAAAAC TCCAATATTC CTCAACGAAG TGCTGGTGAA ACTGCCCACG 
GACCCATCCG GGGATGAGCC AATCTTTCAT ATTTCTCACA TTGACCGAGT CTACACATTG 
CGTGCAGAGA GCATCAATGA GAGGACGGCC TGGGTGCAGA AAATCAAAGC GGCATCGGAG 
TTGTACATAG AGACGGAGAA GAAGAAGAGA GAGAAGGCCT ACTTGGTTCG CTCCCAACGC 
GCAACAGGCA TCGGAAGACT GATGGTAAAT ATCGTTGAGG GAATTGAGTT GAAGCCCTGC 
AGAACCCACG GGAAGAGCAA TCCTTACTGT GAGATCACCA TGGGCTCCCA GTGCCACATT 
ACCAAGACCA TACAGGACAC CCTGAACCCC AAGTGGAACT CCAACTGCCA ATTCTTTATC 
AAGGACCTGG AGCAGGACGT TCTGTGCATC ACCGTGTTTG AGAGGGACCA GTTTTCCCCT 
GACGATTTCC TGGGCCGGAC AGAAATCCGA GTTGCTGACA TAAAGAAAGA CCAAGGTTCT 
AAAGGACCAG TAACCAAGTG TCTACTTCTG CACGAAGTTC CTACTGGAGA GATAGTGGTC 
CGATTGGATC TGCAGCTGTT TGACGAGCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_002939344.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939390.1 Xenopus tropicalis intersectin 1 (itsn1), transcript variant X1, mRNA.

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CloneID	OXa06414
Clone ID Related Accession (Same CDS sequence)	XM_002939344.4 , XM_002939344.5
Accession Version	XM_002939344.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intersectin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939344.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGGGATGA GTGGAATCAG TGGCATTCCT CCCCTAGCAG CTGTAGCCCC TGTGCCAATG 
CCATCCATCC CAGTAGTTGG AATGTCTCCT CCTCTTGTGT CCTCTGTCCC CACAGTGCCA 
CCCTTGGCTA ACGGAGCTCC TGCAGTAATA CAGCCTCTTC CTGCTTTCGC TCACTCTGCC 
ACGTTGCCAA AGAGTTCTTC ATTCGGCCGC TCAGGTGCCG GCTCCCAGAT GAACACTAAA 
CTTCAGAAAG CTCAGTCCTT TGATGTCCCA ACCCCCCCAC CCCTGGCAGA ATGGGCTGTA 
CCACAGTCTT CAAGGTTGAA GTACAGACAG TTGTTCAACA GCCACGACAA AACCATGAGT 
GGGCATTTAA CAGGTCCTCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCGCAGAGC 
CAGCTAGCTA CGATATGGAA CCTTTCAGAC ATTGACCTAG ATGGAAAACT AACGGCCGAA 
GAATTTATTT TAGCTATGCA CTTAATAGAT GTGGCCATGT CTGGCCAGCC ACTTCCTCCA 
GTTCTGCCTC CTGAGTATAT TCCCCCATCT TTTAGAAGAG TTCGATCGGG GAGCGGGTTA 
TCGATTATGA GCTCGGTGTC TGTAGATCAG CGCTTACCGG AGGAACCAGC AGAAGAGGAG 
CCACAGAACA CAGACAAAAG ACTTCCAGTG ACATTTGAAG ACAAGAAAAG GGAAAACTTT 
GAGCGAGGAA ACCTGGAATT AGAAAAGAGG AGACAGGCAC TTCTAGAGCA GCAGCGCAAA 
GAGCAGGAGA GGCTGGCACA GCTGGAAAGG GCAGAGCAGG AGAGAAAAGA ACGAGAGCGG 
CAAGATCAAG AGCGCAAGCG GCAGCAAGAG CTGGAAAAAC AGCTGGAGAA ACAACGAGAG 
CTGGAGCGGC AACGGGAAGA GGAGCGCAGG AAAGAAATAG AAAGGCGAGA GGCAGCAAAG 
AGAGAACTTG AAAGGCAGAG GCAGTTGGAG TGGGAACGAA ACCGGAGGCA AGAACTCCTT 
AATCAAAGGA ACAGAGAGCA AGAGGACATT GTTGTCTTAA AAGCAAAGAA AAAGACTCTT 
GAGTTTGAGC TGGAAGCTCT AAATGATAAA AAGCACCAGC TGGAAGGGAA ACTTCAGGAT 
ATCCGCTGTC GCCTCACTAC TCAACGGCAC GAAATAGAAA GCACTAACAA ATCCAGGGAG 
CTTAGGATCG CAGAGATCAC GCATTTACAG CAGCAGCTTC AGGAATCTCA GCAGCTGCTT 
GGAAAACTGA TTCCTGAGAA ACAGTCCCTT AACGATCAAT TAAAGCAGGT TCAACAAAAC 
AGTTTGCACA GAGATTCCTT GCTGACACTA AAAAGGGCAT TGGAAGCCAA GGAGATGGGC 
CGCCAGCAGC TCCGAGACCA GTTGGATGAA GTAGAAAAAG AAACACGAGC TAAGCTCCAG 
GAAATCGATG TGTTTAATAA CCAATTAAAG GAACTGAGAG AATTGTACAA CAGACAACAG 
CTCCAGAAAC AGAAGGACTT TGAAGCCGAG AAGATCAAGC AGAAAGAGCT GGAACGGAAA 
ACCAGTGAGC TGGACAAGAT GAAAGAGGAG GACAAAAGGC GGATGCTAGA GCAGGATAAG 
CTTTGGCAGG ATCGTGTGAA GCAGGAAGAG GAACGTTACA AATTCCAAGA TGAGGAGAAG 
CAGAAAAGGG AAGAATCCGC TCAACAATGT GAGGAAGAAA AGAAACCAGA GATCCAAGAG 
AAGCCCAACA AACTGTTCCA CCTACCACCG GAGCCAGGAA AGCTAGGAGG GCATGTCCCC 
TGGATGAATA CAGAGAAAGC ACCGCTAACA TTCAACCAGG GTGATGTTAA GGTTGTGTAT 
TACCGGGCAC TCTACCCTTT TGATGCTCGA AGTCATGATG AAATCACTAT TCAGCCTGGG 
GACATTATTA TGGTGGATGA GAGCCAAACC GGAGAGCCTG GATGGCTGGG AGGTGAACTG 
AAGGGAAAAA CTGGCTGGTT TCCTGCCAAC TATGCAGAGA GAATGCCCGA GGGTGAATTC 
CCAAGCACAA CTAAACAGGC AGCAGACACT ACGGCCAAAC CCACTGTCCA TTTGGCACCT 
TCCACTCCCA CACCTGCAGC TTTTACAGAT AACTCTGCAA ATTCCAACAA CTGGGCAGAC 
TTCAGCTCCA CGTGGCCAAC CAATAACACT GATAAAGCAG AGACGGATAA CTGGGATACA 
TGGGGAGCTC AACCATCTCT GACTGTTCCT AGTGCCGGAC AGCACAGGCA GCGCTCTGCT 
TTTACCCCAG CAACGGTTAC TGGATCATCT CCATCTCCTG TACTTGGTCA GGGTGAAAAA 
GTAGAAGGGC TTCAGGCACA AGCCTTGTAT CCTTGGAGAG CAAAAAAGGA CAACCACCTT 
AATTTTAACA AAAATGACAT CATCACGGTT CTAGAGCAGC AGGATATGTG GTGGTTTGGG 
GAAGTTCAAG GTCAAAAGGG CTGGTTTCCC AAATCCTACG TAAAACTTAT ATCCGGGCCA 
CTAAGGAAAT CTACAAGCAT CGATTCCACA TCTTCAGAAA GCCCTGCTAG TTTAAAAAGA 
GTCTCTTCAC CAGCATTTAA ACCTGCCATG CAAGGAGAAG AATACATAGC CATGTACACG 
TACGAGAGCA ACGAGCAGGG AGACTTGACC TTTCAGCAAG GAGATCTGAT TGTGGTGATT 
AAAAAAGATG GGGACTGGTG GACTGGCACG GTGGGAGAGA AGACGGGGGT TTTCCCTTCC 
AACTACGTAA GGCCCAAAGA CTCCGAGGCG GCTGGAAGCG CAGGAAAGAC AGGAAGCCTT 
GGGAAGAAAC CAGAAATTGC CCAGGTTATT GCTTCTTACA CAGCAACTGG CCCTGAGCAG 
CTGACACTGG CTCCTGGCCA ACTTATCCTC ATCCGGAAAA AGAATCCTGG AGGATGGTGG 
GAAGGAGAAC TGCAGGCACG TGGGAAGAAG CGACAGATTG GATGGTTTCC AGCAAATTAT 
GTGAAGCTTT TGAGCCCTGG TACAAACAAA AGCACGCCGA CAGAACCACC AAAAGCAACA 
TCATTGCCTC CAACATGCCA AGTCATCGGG ATGTACGATT ATATCGCTCA GAATGACGAT 
GAGTTGGCCT TCGGTAAAGG TCAGGTCATT AACGTGCTGA ACAAAGAGGA CCCGGACTGG 
TGGAAAGGAG AACTGAACGG CCATGTTGGT CTGTTTCCCT CCAACTATGT GAAACTGACC 
ACAGACATGG ATCCCAGTCA GCAATGGTGT GCAGACCTAC ATCTCCTGGA CATGCTGAGT 
CCCACCGAGA GGAAGAGGCA AGGGTACATC CACGAGCTCA TTGTCACCGA GGAGAACTAT 
GTCAATGACT TACAGTTAGT CACCGAGACA TTCCAAAAGC CTCTGCTGGA ATCTGAATTA 
CTGACGGAGA AGGAAGTTGC CATGATTTTT GTCAACTGGA AGGAGCTGAT AATGTGCAAT 
ATCAAACTGC TCAAGGCCTT GCGAGTTCGC AAGAAAATGT CTGGGGAGAA GATGCCGGTG 
AAGATGATCG GGGACATCCT GACGGCCCAG TTGCCCCACA TGCAGCCGTA CATTCGCTTC 
TGCAGCTGCC AGCTCAACGG CGCCGCTCTC ATTCAGCAGA AAACGGACGA GGTGCCCGAG 
TTCAAGGAGT TTGTGAAAAG GCTTGCAATG GATCCCCGGT GTAAGGGAAT GCCGCTGTCC 
AGCTTCCTAC TCAAACCCAT GCAGAGAGTA ACCAGATACC CGCTCATCAT TAAAAATATT 
ATTGAAAATA CTCCAGAAAA TCACCCTGAC CACAGCCACC TGAAGCAAGC CCTAGAGAAA 
GCCGAAGAAT TATGTTCCCA AGTGAATGAG GGGGTTCGGG AGAAGGAAAA CTCAGACCGA 
CTGGAGTGGA TTCAGGCGCA CGTTCAGTGT GAGGGTTTGT CTGAGCAATT AGTATTTAAC 
TCTGTTACAA ACTGCCTGGG CCCACGCAAG TTCCTGCACA GTGGGAAACT GTACAAAGCC 
AAGAGCAACA AGGAACTATA CGGCTTCCTG TTCAACGACT TCCTGCTGCT CACCCAGATC 
ATTAAGCCCC TCGGCTCCTC CGGCAACGAT AAGGTGTTCA GCCCCAAGTC CAATCTCCAG 
TACAAGATGT ACAAAACTCC AATATTCCTC AACGAAGTGC TGGTGAAACT GCCCACGGAC 
CCATCCGGGG ATGAGCCAAT CTTTCATATT TCTCACATTG ACCGAGTCTA CACATTGCGT 
GCAGAGAGCA TCAATGAGAG GACGGCCTGG GTGCAGAAAA TCAAAGCGGC ATCGGAGTTG 
TACATAGAGA CGGAGAAGAA GAAGAGAGAG AAGGCCTACT TGGTTCGCTC CCAACGCGCA 
ACAGGCATCG GAAGACTGAT GGTAAATATC GTTGAGGGAA TTGAGTTGAA GCCCTGCAGA 
ACCCACGGGA AGAGCAATCC TTACTGTGAG ATCACCATGG GCTCCCAGTG CCACATTACC 
AAGACCATAC AGGACACCCT GAACCCCAAG TGGAACTCCA ACTGCCAATT CTTTATCAAG 
GACCTGGAGC AGGACGTTCT GTGCATCACC GTGTTTGAGA GGGACCAGTT TTCCCCTGAC 
GATTTCCTGG GCCGGACAGA AATCCGAGTT GCTGACATAA AGAAAGACCA AGGTTCTAAA 
GGACCAGTAA CCAAGTGTCT ACTTCTGCAC GAAGTTCCTA CTGGAGAGAT AGTGGTCCGA 
TTGGATCTGC AGCTGTTTGA CGAGCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939390.1
CDS	301..5430
Translation	MAQFGTPFGGNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSAAAAAAAAAGFGMSGISGIPPLAAVAPVPMPSIPVVGMSPPLVSSVPTVPPLANGAPAVIQPLPAFAHSATLPKSSSFGRSGAGSQMNTKLQKAQSFDVPTPPPLAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGLSIMSSVSVDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRELRIAEITHLQQQLQESQQLLGKLIPEKQSLNDQLKQVQQNSLHRDSLLTLKRALEAKEMGRQQLRDQLDEVEKETRAKLQEIDVFNNQLKELRELYNRQQLQKQKDFEAEKIKQKELERKTSELDKMKEEDKRRMLEQDKLWQDRVKQEEERYKFQDEEKQKREESAQQCEEEKKPEIQEKPNKLFHLPPEPGKLGGHVPWMNTEKAPLTFNQGDVKVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEPGWLGGELKGKTGWFPANYAERMPEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTWPTNNTDKAETDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVTGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRVSSPAFKPAMQGEEYIAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTNKSTPTEPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGELNGHVGLFPSNYVKLTTDMDPSQQWCADLHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_002939344.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939344.4

ORF Insert Sequence:

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGGGATGA GTGGAATCAG TGGCATTCCT CCCCTAGCAG CTGTAGCCCC TGTGCCAATG 
CCATCCATCC CAGTAGTTGG AATGTCTCCT CCTCTTGTGT CCTCTGTCCC CACAGTGCCA 
CCCTTGGCTA ACGGAGCTCC TGCAGTAATA CAGCCTCTTC CTGCTTTCGC TCACTCTGCC 
ACGTTGCCAA AGAGTTCTTC ATTCGGCCGC TCAGGTGCCG GCTCCCAGAT GAACACTAAA 
CTTCAGAAAG CTCAGTCCTT TGATGTCCCA ACCCCCCCAC CCCTGGCAGA ATGGGCTGTA 
CCACAGTCTT CAAGGTTGAA GTACAGACAG TTGTTCAACA GCCACGACAA AACCATGAGT 
GGGCATTTAA CAGGTCCTCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCGCAGAGC 
CAGCTAGCTA CGATATGGAA CCTTTCAGAC ATTGACCTAG ATGGAAAACT AACGGCCGAA 
GAATTTATTT TAGCTATGCA CTTAATAGAT GTGGCCATGT CTGGCCAGCC ACTTCCTCCA 
GTTCTGCCTC CTGAGTATAT TCCCCCATCT TTTAGAAGAG TTCGATCGGG GAGCGGGTTA 
TCGATTATGA GCTCGGTGTC TGTAGATCAG CGCTTACCGG AGGAACCAGC AGAAGAGGAG 
CCACAGAACA CAGACAAAAG ACTTCCAGTG ACATTTGAAG ACAAGAAAAG GGAAAACTTT 
GAGCGAGGAA ACCTGGAATT AGAAAAGAGG AGACAGGCAC TTCTAGAGCA GCAGCGCAAA 
GAGCAGGAGA GGCTGGCACA GCTGGAAAGG GCAGAGCAGG AGAGAAAAGA ACGAGAGCGG 
CAAGATCAAG AGCGCAAGCG GCAGCAAGAG CTGGAAAAAC AGCTGGAGAA ACAACGAGAG 
CTGGAGCGGC AACGGGAAGA GGAGCGCAGG AAAGAAATAG AAAGGCGAGA GGCAGCAAAG 
AGAGAACTTG AAAGGCAGAG GCAGTTGGAG TGGGAACGAA ACCGGAGGCA AGAACTCCTT 
AATCAAAGGA ACAGAGAGCA AGAGGACATT GTTGTCTTAA AAGCAAAGAA AAAGACTCTT 
GAGTTTGAGC TGGAAGCTCT AAATGATAAA AAGCACCAGC TGGAAGGGAA ACTTCAGGAT 
ATCCGCTGTC GCCTCACTAC TCAACGGCAC GAAATAGAAA GCACTAACAA ATCCAGGGAG 
CTTAGGATCG CAGAGATCAC GCATTTACAG CAGCAGCTTC AGGAATCTCA GCAGCTGCTT 
GGAAAACTGA TTCCTGAGAA ACAGTCCCTT AACGATCAAT TAAAGCAGGT TCAACAAAAC 
AGTTTGCACA GAGATTCCTT GCTGACACTA AAAAGGGCAT TGGAAGCCAA GGAGATGGGC 
CGCCAGCAGC TCCGAGACCA GTTGGATGAA GTAGAAAAAG AAACACGAGC TAAGCTCCAG 
GAAATCGATG TGTTTAATAA CCAATTAAAG GAACTGAGAG AATTGTACAA CAGACAACAG 
CTCCAGAAAC AGAAGGACTT TGAAGCCGAG AAGATCAAGC AGAAAGAGCT GGAACGGAAA 
ACCAGTGAGC TGGACAAGAT GAAAGAGGAG GACAAAAGGC GGATGCTAGA GCAGGATAAG 
CTTTGGCAGG ATCGTGTGAA GCAGGAAGAG GAACGTTACA AATTCCAAGA TGAGGAGAAG 
CAGAAAAGGG AAGAATCCGC TCAACAATGT GAGGAAGAAA AGAAACCAGA GATCCAAGAG 
AAGCCCAACA AACTGTTCCA CCTACCACCG GAGCCAGGAA AGCTAGGAGG GCATGTCCCC 
TGGATGAATA CAGAGAAAGC ACCGCTAACA TTCAACCAGG GTGATGTTAA GGTTGTGTAT 
TACCGGGCAC TCTACCCTTT TGATGCTCGA AGTCATGATG AAATCACTAT TCAGCCTGGG 
GACATTATTA TGGTGGATGA GAGCCAAACC GGAGAGCCTG GATGGCTGGG AGGTGAACTG 
AAGGGAAAAA CTGGCTGGTT TCCTGCCAAC TATGCAGAGA GAATGCCCGA GGGTGAATTC 
CCAAGCACAA CTAAACAGGC AGCAGACACT ACGGCCAAAC CCACTGTCCA TTTGGCACCT 
TCCACTCCCA CACCTGCAGC TTTTACAGAT AACTCTGCAA ATTCCAACAA CTGGGCAGAC 
TTCAGCTCCA CGTGGCCAAC CAATAACACT GATAAAGCAG AGACGGATAA CTGGGATACA 
TGGGGAGCTC AACCATCTCT GACTGTTCCT AGTGCCGGAC AGCACAGGCA GCGCTCTGCT 
TTTACCCCAG CAACGGTTAC TGGATCATCT CCATCTCCTG TACTTGGTCA GGGTGAAAAA 
GTAGAAGGGC TTCAGGCACA AGCCTTGTAT CCTTGGAGAG CAAAAAAGGA CAACCACCTT 
AATTTTAACA AAAATGACAT CATCACGGTT CTAGAGCAGC AGGATATGTG GTGGTTTGGG 
GAAGTTCAAG GTCAAAAGGG CTGGTTTCCC AAATCCTACG TAAAACTTAT ATCCGGGCCA 
CTAAGGAAAT CTACAAGCAT CGATTCCACA TCTTCAGAAA GCCCTGCTAG TTTAAAAAGA 
GTCTCTTCAC CAGCATTTAA ACCTGCCATG CAAGGAGAAG AATACATAGC CATGTACACG 
TACGAGAGCA ACGAGCAGGG AGACTTGACC TTTCAGCAAG GAGATCTGAT TGTGGTGATT 
AAAAAAGATG GGGACTGGTG GACTGGCACG GTGGGAGAGA AGACGGGGGT TTTCCCTTCC 
AACTACGTAA GGCCCAAAGA CTCCGAGGCG GCTGGAAGCG CAGGAAAGAC AGGAAGCCTT 
GGGAAGAAAC CAGAAATTGC CCAGGTTATT GCTTCTTACA CAGCAACTGG CCCTGAGCAG 
CTGACACTGG CTCCTGGCCA ACTTATCCTC ATCCGGAAAA AGAATCCTGG AGGATGGTGG 
GAAGGAGAAC TGCAGGCACG TGGGAAGAAG CGACAGATTG GATGGTTTCC AGCAAATTAT 
GTGAAGCTTT TGAGCCCTGG TACAAACAAA AGCACGCCGA CAGAACCACC AAAAGCAACA 
TCATTGCCTC CAACATGCCA AGTCATCGGG ATGTACGATT ATATCGCTCA GAATGACGAT 
GAGTTGGCCT TCGGTAAAGG TCAGGTCATT AACGTGCTGA ACAAAGAGGA CCCGGACTGG 
TGGAAAGGAG AACTGAACGG CCATGTTGGT CTGTTTCCCT CCAACTATGT GAAACTGACC 
ACAGACATGG ATCCCAGTCA GCAATGGTGT GCAGACCTAC ATCTCCTGGA CATGCTGAGT 
CCCACCGAGA GGAAGAGGCA AGGGTACATC CACGAGCTCA TTGTCACCGA GGAGAACTAT 
GTCAATGACT TACAGTTAGT CACCGAGACA TTCCAAAAGC CTCTGCTGGA ATCTGAATTA 
CTGACGGAGA AGGAAGTTGC CATGATTTTT GTCAACTGGA AGGAGCTGAT AATGTGCAAT 
ATCAAACTGC TCAAGGCCTT GCGAGTTCGC AAGAAAATGT CTGGGGAGAA GATGCCGGTG 
AAGATGATCG GGGACATCCT GACGGCCCAG TTGCCCCACA TGCAGCCGTA CATTCGCTTC 
TGCAGCTGCC AGCTCAACGG CGCCGCTCTC ATTCAGCAGA AAACGGACGA GGTGCCCGAG 
TTCAAGGAGT TTGTGAAAAG GCTTGCAATG GATCCCCGGT GTAAGGGAAT GCCGCTGTCC 
AGCTTCCTAC TCAAACCCAT GCAGAGAGTA ACCAGATACC CGCTCATCAT TAAAAATATT 
ATTGAAAATA CTCCAGAAAA TCACCCTGAC CACAGCCACC TGAAGCAAGC CCTAGAGAAA 
GCCGAAGAAT TATGTTCCCA AGTGAATGAG GGGGTTCGGG AGAAGGAAAA CTCAGACCGA 
CTGGAGTGGA TTCAGGCGCA CGTTCAGTGT GAGGGTTTGT CTGAGCAATT AGTATTTAAC 
TCTGTTACAA ACTGCCTGGG CCCACGCAAG TTCCTGCACA GTGGGAAACT GTACAAAGCC 
AAGAGCAACA AGGAACTATA CGGCTTCCTG TTCAACGACT TCCTGCTGCT CACCCAGATC 
ATTAAGCCCC TCGGCTCCTC CGGCAACGAT AAGGTGTTCA GCCCCAAGTC CAATCTCCAG 
TACAAGATGT ACAAAACTCC AATATTCCTC AACGAAGTGC TGGTGAAACT GCCCACGGAC 
CCATCCGGGG ATGAGCCAAT CTTTCATATT TCTCACATTG ACCGAGTCTA CACATTGCGT 
GCAGAGAGCA TCAATGAGAG GACGGCCTGG GTGCAGAAAA TCAAAGCGGC ATCGGAGTTG 
TACATAGAGA CGGAGAAGAA GAAGAGAGAG AAGGCCTACT TGGTTCGCTC CCAACGCGCA 
ACAGGCATCG GAAGACTGAT GGTAAATATC GTTGAGGGAA TTGAGTTGAA GCCCTGCAGA 
ACCCACGGGA AGAGCAATCC TTACTGTGAG ATCACCATGG GCTCCCAGTG CCACATTACC 
AAGACCATAC AGGACACCCT GAACCCCAAG TGGAACTCCA ACTGCCAATT CTTTATCAAG 
GACCTGGAGC AGGACGTTCT GTGCATCACC GTGTTTGAGA GGGACCAGTT TTCCCCTGAC 
GATTTCCTGG GCCGGACAGA AATCCGAGTT GCTGACATAA AGAAAGACCA AGGTTCTAAA 
GGACCAGTAA CCAAGTGTCT ACTTCTGCAC GAAGTTCCTA CTGGAGAGAT AGTGGTCCGA 
TTGGATCTGC AGCTGTTTGA CGAGCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_004912127.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912184.1 Xenopus tropicalis intersectin 1 (itsn1), transcript variant X2, mRNA.

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CloneID	OXa06415
Clone ID Related Accession (Same CDS sequence)	XM_004912127.3 , XM_004912127.4
Accession Version	XM_004912127.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intersectin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912127.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGGGATGA GTGGAATCAG TGGCATTCCT CCCCTAGCAG CTGTAGCCCC TGTGCCAATG 
CCATCCATCC CAGTAGTTGG AATGTCTCCT CCTCTTGTGT CCTCTGTCCC CACAGTGCCA 
CCCTTGGCTA ACGGAGCTCC TGCAGTAATA CAGCCTCTTC CTGCTTTCGC TCACTCTGCC 
ACGTTGCCAA AGAGTTCTTC ATTCGGCCGC TCAGGTGCCG GCTCCCAGAT GAACACTAAA 
CTTCAGAAAG CTCAGTCCTT TGATGTCCCA ACCCCCCCAC CCCTGGCAGA ATGGGCTGTA 
CCACAGTCTT CAAGGTTGAA GTACAGACAG TTGTTCAACA GCCACGACAA AACCATGAGT 
GGGCATTTAA CAGGTCCTCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCGCAGAGC 
CAGCTAGCTA CGATATGGAA CCTTTCAGAC ATTGACCTAG ATGGAAAACT AACGGCCGAA 
GAATTTATTT TAGCTATGCA CTTAATAGAT GTGGCCATGT CTGGCCAGCC ACTTCCTCCA 
GTTCTGCCTC CTGAGTATAT TCCCCCATCT TTTAGAGTTC GATCGGGGAG CGGGTTATCG 
ATTATGAGCT CGGTGTCTGT AGATCAGCGC TTACCGGAGG AACCAGCAGA AGAGGAGCCA 
CAGAACACAG ACAAAAGACT TCCAGTGACA TTTGAAGACA AGAAAAGGGA AAACTTTGAG 
CGAGGAAACC TGGAATTAGA AAAGAGGAGA CAGGCACTTC TAGAGCAGCA GCGCAAAGAG 
CAGGAGAGGC TGGCACAGCT GGAAAGGGCA GAGCAGGAGA GAAAAGAACG AGAGCGGCAA 
GATCAAGAGC GCAAGCGGCA GCAAGAGCTG GAAAAACAGC TGGAGAAACA ACGAGAGCTG 
GAGCGGCAAC GGGAAGAGGA GCGCAGGAAA GAAATAGAAA GGCGAGAGGC AGCAAAGAGA 
GAACTTGAAA GGCAGAGGCA GTTGGAGTGG GAACGAAACC GGAGGCAAGA ACTCCTTAAT 
CAAAGGAACA GAGAGCAAGA GGACATTGTT GTCTTAAAAG CAAAGAAAAA GACTCTTGAG 
TTTGAGCTGG AAGCTCTAAA TGATAAAAAG CACCAGCTGG AAGGGAAACT TCAGGATATC 
CGCTGTCGCC TCACTACTCA ACGGCACGAA ATAGAAAGCA CTAACAAATC CAGGGAGCTT 
AGGATCGCAG AGATCACGCA TTTACAGCAG CAGCTTCAGG AATCTCAGCA GCTGCTTGGA 
AAACTGATTC CTGAGAAACA GTCCCTTAAC GATCAATTAA AGCAGGTTCA ACAAAACAGT 
TTGCACAGAG ATTCCTTGCT GACACTAAAA AGGGCATTGG AAGCCAAGGA GATGGGCCGC 
CAGCAGCTCC GAGACCAGTT GGATGAAGTA GAAAAAGAAA CACGAGCTAA GCTCCAGGAA 
ATCGATGTGT TTAATAACCA ATTAAAGGAA CTGAGAGAAT TGTACAACAG ACAACAGCTC 
CAGAAACAGA AGGACTTTGA AGCCGAGAAG ATCAAGCAGA AAGAGCTGGA ACGGAAAACC 
AGTGAGCTGG ACAAGATGAA AGAGGAGGAC AAAAGGCGGA TGCTAGAGCA GGATAAGCTT 
TGGCAGGATC GTGTGAAGCA GGAAGAGGAA CGTTACAAAT TCCAAGATGA GGAGAAGCAG 
AAAAGGGAAG AATCCGCTCA ACAATGTGAG GAAGAAAAGA AACCAGAGAT CCAAGAGAAG 
CCCAACAAAC TGTTCCACCT ACCACCGGAG CCAGGAAAGC TAGGAGGGCA TGTCCCCTGG 
ATGAATACAG AGAAAGCACC GCTAACATTC AACCAGGGTG ATGTTAAGGT TGTGTATTAC 
CGGGCACTCT ACCCTTTTGA TGCTCGAAGT CATGATGAAA TCACTATTCA GCCTGGGGAC 
ATTATTATGG TGGATGAGAG CCAAACCGGA GAGCCTGGAT GGCTGGGAGG TGAACTGAAG 
GGAAAAACTG GCTGGTTTCC TGCCAACTAT GCAGAGAGAA TGCCCGAGGG TGAATTCCCA 
AGCACAACTA AACAGGCAGC AGACACTACG GCCAAACCCA CTGTCCATTT GGCACCTTCC 
ACTCCCACAC CTGCAGCTTT TACAGATAAC TCTGCAAATT CCAACAACTG GGCAGACTTC 
AGCTCCACGT GGCCAACCAA TAACACTGAT AAAGCAGAGA CGGATAACTG GGATACATGG 
GGAGCTCAAC CATCTCTGAC TGTTCCTAGT GCCGGACAGC ACAGGCAGCG CTCTGCTTTT 
ACCCCAGCAA CGGTTACTGG ATCATCTCCA TCTCCTGTAC TTGGTCAGGG TGAAAAAGTA 
GAAGGGCTTC AGGCACAAGC CTTGTATCCT TGGAGAGCAA AAAAGGACAA CCACCTTAAT 
TTTAACAAAA ATGACATCAT CACGGTTCTA GAGCAGCAGG ATATGTGGTG GTTTGGGGAA 
GTTCAAGGTC AAAAGGGCTG GTTTCCCAAA TCCTACGTAA AACTTATATC CGGGCCACTA 
AGGAAATCTA CAAGCATCGA TTCCACATCT TCAGAAAGCC CTGCTAGTTT AAAAAGAGTC 
TCTTCACCAG CATTTAAACC TGCCATGCAA GGAGAAGAAT ACATAGCCAT GTACACGTAC 
GAGAGCAACG AGCAGGGAGA CTTGACCTTT CAGCAAGGAG ATCTGATTGT GGTGATTAAA 
AAAGATGGGG ACTGGTGGAC TGGCACGGTG GGAGAGAAGA CGGGGGTTTT CCCTTCCAAC 
TACGTAAGGC CCAAAGACTC CGAGGCGGCT GGAAGCGCAG GAAAGACAGG AAGCCTTGGG 
AAGAAACCAG AAATTGCCCA GGTTATTGCT TCTTACACAG CAACTGGCCC TGAGCAGCTG 
ACACTGGCTC CTGGCCAACT TATCCTCATC CGGAAAAAGA ATCCTGGAGG ATGGTGGGAA 
GGAGAACTGC AGGCACGTGG GAAGAAGCGA CAGATTGGAT GGTTTCCAGC AAATTATGTG 
AAGCTTTTGA GCCCTGGTAC AAACAAAAGC ACGCCGACAG AACCACCAAA AGCAACATCA 
TTGCCTCCAA CATGCCAAGT CATCGGGATG TACGATTATA TCGCTCAGAA TGACGATGAG 
TTGGCCTTCG GTAAAGGTCA GGTCATTAAC GTGCTGAACA AAGAGGACCC GGACTGGTGG 
AAAGGAGAAC TGAACGGCCA TGTTGGTCTG TTTCCCTCCA ACTATGTGAA ACTGACCACA 
GACATGGATC CCAGTCAGCA ATGGTGTGCA GACCTACATC TCCTGGACAT GCTGAGTCCC 
ACCGAGAGGA AGAGGCAAGG GTACATCCAC GAGCTCATTG TCACCGAGGA GAACTATGTC 
AATGACTTAC AGTTAGTCAC CGAGACATTC CAAAAGCCTC TGCTGGAATC TGAATTACTG 
ACGGAGAAGG AAGTTGCCAT GATTTTTGTC AACTGGAAGG AGCTGATAAT GTGCAATATC 
AAACTGCTCA AGGCCTTGCG AGTTCGCAAG AAAATGTCTG GGGAGAAGAT GCCGGTGAAG 
ATGATCGGGG ACATCCTGAC GGCCCAGTTG CCCCACATGC AGCCGTACAT TCGCTTCTGC 
AGCTGCCAGC TCAACGGCGC CGCTCTCATT CAGCAGAAAA CGGACGAGGT GCCCGAGTTC 
AAGGAGTTTG TGAAAAGGCT TGCAATGGAT CCCCGGTGTA AGGGAATGCC GCTGTCCAGC 
TTCCTACTCA AACCCATGCA GAGAGTAACC AGATACCCGC TCATCATTAA AAATATTATT 
GAAAATACTC CAGAAAATCA CCCTGACCAC AGCCACCTGA AGCAAGCCCT AGAGAAAGCC 
GAAGAATTAT GTTCCCAAGT GAATGAGGGG GTTCGGGAGA AGGAAAACTC AGACCGACTG 
GAGTGGATTC AGGCGCACGT TCAGTGTGAG GGTTTGTCTG AGCAATTAGT ATTTAACTCT 
GTTACAAACT GCCTGGGCCC ACGCAAGTTC CTGCACAGTG GGAAACTGTA CAAAGCCAAG 
AGCAACAAGG AACTATACGG CTTCCTGTTC AACGACTTCC TGCTGCTCAC CCAGATCATT 
AAGCCCCTCG GCTCCTCCGG CAACGATAAG GTGTTCAGCC CCAAGTCCAA TCTCCAGTAC 
AAGATGTACA AAACTCCAAT ATTCCTCAAC GAAGTGCTGG TGAAACTGCC CACGGACCCA 
TCCGGGGATG AGCCAATCTT TCATATTTCT CACATTGACC GAGTCTACAC ATTGCGTGCA 
GAGAGCATCA ATGAGAGGAC GGCCTGGGTG CAGAAAATCA AAGCGGCATC GGAGTTGTAC 
ATAGAGACGG AGAAGAAGAA GAGAGAGAAG GCCTACTTGG TTCGCTCCCA ACGCGCAACA 
GGCATCGGAA GACTGATGGT AAATATCGTT GAGGGAATTG AGTTGAAGCC CTGCAGAACC 
CACGGGAAGA GCAATCCTTA CTGTGAGATC ACCATGGGCT CCCAGTGCCA CATTACCAAG 
ACCATACAGG ACACCCTGAA CCCCAAGTGG AACTCCAACT GCCAATTCTT TATCAAGGAC 
CTGGAGCAGG ACGTTCTGTG CATCACCGTG TTTGAGAGGG ACCAGTTTTC CCCTGACGAT 
TTCCTGGGCC GGACAGAAAT CCGAGTTGCT GACATAAAGA AAGACCAAGG TTCTAAAGGA 
CCAGTAACCA AGTGTCTACT TCTGCACGAA GTTCCTACTG GAGAGATAGT GGTCCGATTG 
GATCTGCAGC TGTTTGACGA GCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912184.1
CDS	301..5427
Translation	MAQFGTPFGGNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSAAAAAAAAAGFGMSGISGIPPLAAVAPVPMPSIPVVGMSPPLVSSVPTVPPLANGAPAVIQPLPAFAHSATLPKSSSFGRSGAGSQMNTKLQKAQSFDVPTPPPLAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRVRSGSGLSIMSSVSVDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRELRIAEITHLQQQLQESQQLLGKLIPEKQSLNDQLKQVQQNSLHRDSLLTLKRALEAKEMGRQQLRDQLDEVEKETRAKLQEIDVFNNQLKELRELYNRQQLQKQKDFEAEKIKQKELERKTSELDKMKEEDKRRMLEQDKLWQDRVKQEEERYKFQDEEKQKREESAQQCEEEKKPEIQEKPNKLFHLPPEPGKLGGHVPWMNTEKAPLTFNQGDVKVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEPGWLGGELKGKTGWFPANYAERMPEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTWPTNNTDKAETDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVTGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRVSSPAFKPAMQGEEYIAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTNKSTPTEPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGELNGHVGLFPSNYVKLTTDMDPSQQWCADLHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_004912127.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912127.3

ORF Insert Sequence:

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGGGATGA GTGGAATCAG TGGCATTCCT CCCCTAGCAG CTGTAGCCCC TGTGCCAATG 
CCATCCATCC CAGTAGTTGG AATGTCTCCT CCTCTTGTGT CCTCTGTCCC CACAGTGCCA 
CCCTTGGCTA ACGGAGCTCC TGCAGTAATA CAGCCTCTTC CTGCTTTCGC TCACTCTGCC 
ACGTTGCCAA AGAGTTCTTC ATTCGGCCGC TCAGGTGCCG GCTCCCAGAT GAACACTAAA 
CTTCAGAAAG CTCAGTCCTT TGATGTCCCA ACCCCCCCAC CCCTGGCAGA ATGGGCTGTA 
CCACAGTCTT CAAGGTTGAA GTACAGACAG TTGTTCAACA GCCACGACAA AACCATGAGT 
GGGCATTTAA CAGGTCCTCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCGCAGAGC 
CAGCTAGCTA CGATATGGAA CCTTTCAGAC ATTGACCTAG ATGGAAAACT AACGGCCGAA 
GAATTTATTT TAGCTATGCA CTTAATAGAT GTGGCCATGT CTGGCCAGCC ACTTCCTCCA 
GTTCTGCCTC CTGAGTATAT TCCCCCATCT TTTAGAGTTC GATCGGGGAG CGGGTTATCG 
ATTATGAGCT CGGTGTCTGT AGATCAGCGC TTACCGGAGG AACCAGCAGA AGAGGAGCCA 
CAGAACACAG ACAAAAGACT TCCAGTGACA TTTGAAGACA AGAAAAGGGA AAACTTTGAG 
CGAGGAAACC TGGAATTAGA AAAGAGGAGA CAGGCACTTC TAGAGCAGCA GCGCAAAGAG 
CAGGAGAGGC TGGCACAGCT GGAAAGGGCA GAGCAGGAGA GAAAAGAACG AGAGCGGCAA 
GATCAAGAGC GCAAGCGGCA GCAAGAGCTG GAAAAACAGC TGGAGAAACA ACGAGAGCTG 
GAGCGGCAAC GGGAAGAGGA GCGCAGGAAA GAAATAGAAA GGCGAGAGGC AGCAAAGAGA 
GAACTTGAAA GGCAGAGGCA GTTGGAGTGG GAACGAAACC GGAGGCAAGA ACTCCTTAAT 
CAAAGGAACA GAGAGCAAGA GGACATTGTT GTCTTAAAAG CAAAGAAAAA GACTCTTGAG 
TTTGAGCTGG AAGCTCTAAA TGATAAAAAG CACCAGCTGG AAGGGAAACT TCAGGATATC 
CGCTGTCGCC TCACTACTCA ACGGCACGAA ATAGAAAGCA CTAACAAATC CAGGGAGCTT 
AGGATCGCAG AGATCACGCA TTTACAGCAG CAGCTTCAGG AATCTCAGCA GCTGCTTGGA 
AAACTGATTC CTGAGAAACA GTCCCTTAAC GATCAATTAA AGCAGGTTCA ACAAAACAGT 
TTGCACAGAG ATTCCTTGCT GACACTAAAA AGGGCATTGG AAGCCAAGGA GATGGGCCGC 
CAGCAGCTCC GAGACCAGTT GGATGAAGTA GAAAAAGAAA CACGAGCTAA GCTCCAGGAA 
ATCGATGTGT TTAATAACCA ATTAAAGGAA CTGAGAGAAT TGTACAACAG ACAACAGCTC 
CAGAAACAGA AGGACTTTGA AGCCGAGAAG ATCAAGCAGA AAGAGCTGGA ACGGAAAACC 
AGTGAGCTGG ACAAGATGAA AGAGGAGGAC AAAAGGCGGA TGCTAGAGCA GGATAAGCTT 
TGGCAGGATC GTGTGAAGCA GGAAGAGGAA CGTTACAAAT TCCAAGATGA GGAGAAGCAG 
AAAAGGGAAG AATCCGCTCA ACAATGTGAG GAAGAAAAGA AACCAGAGAT CCAAGAGAAG 
CCCAACAAAC TGTTCCACCT ACCACCGGAG CCAGGAAAGC TAGGAGGGCA TGTCCCCTGG 
ATGAATACAG AGAAAGCACC GCTAACATTC AACCAGGGTG ATGTTAAGGT TGTGTATTAC 
CGGGCACTCT ACCCTTTTGA TGCTCGAAGT CATGATGAAA TCACTATTCA GCCTGGGGAC 
ATTATTATGG TGGATGAGAG CCAAACCGGA GAGCCTGGAT GGCTGGGAGG TGAACTGAAG 
GGAAAAACTG GCTGGTTTCC TGCCAACTAT GCAGAGAGAA TGCCCGAGGG TGAATTCCCA 
AGCACAACTA AACAGGCAGC AGACACTACG GCCAAACCCA CTGTCCATTT GGCACCTTCC 
ACTCCCACAC CTGCAGCTTT TACAGATAAC TCTGCAAATT CCAACAACTG GGCAGACTTC 
AGCTCCACGT GGCCAACCAA TAACACTGAT AAAGCAGAGA CGGATAACTG GGATACATGG 
GGAGCTCAAC CATCTCTGAC TGTTCCTAGT GCCGGACAGC ACAGGCAGCG CTCTGCTTTT 
ACCCCAGCAA CGGTTACTGG ATCATCTCCA TCTCCTGTAC TTGGTCAGGG TGAAAAAGTA 
GAAGGGCTTC AGGCACAAGC CTTGTATCCT TGGAGAGCAA AAAAGGACAA CCACCTTAAT 
TTTAACAAAA ATGACATCAT CACGGTTCTA GAGCAGCAGG ATATGTGGTG GTTTGGGGAA 
GTTCAAGGTC AAAAGGGCTG GTTTCCCAAA TCCTACGTAA AACTTATATC CGGGCCACTA 
AGGAAATCTA CAAGCATCGA TTCCACATCT TCAGAAAGCC CTGCTAGTTT AAAAAGAGTC 
TCTTCACCAG CATTTAAACC TGCCATGCAA GGAGAAGAAT ACATAGCCAT GTACACGTAC 
GAGAGCAACG AGCAGGGAGA CTTGACCTTT CAGCAAGGAG ATCTGATTGT GGTGATTAAA 
AAAGATGGGG ACTGGTGGAC TGGCACGGTG GGAGAGAAGA CGGGGGTTTT CCCTTCCAAC 
TACGTAAGGC CCAAAGACTC CGAGGCGGCT GGAAGCGCAG GAAAGACAGG AAGCCTTGGG 
AAGAAACCAG AAATTGCCCA GGTTATTGCT TCTTACACAG CAACTGGCCC TGAGCAGCTG 
ACACTGGCTC CTGGCCAACT TATCCTCATC CGGAAAAAGA ATCCTGGAGG ATGGTGGGAA 
GGAGAACTGC AGGCACGTGG GAAGAAGCGA CAGATTGGAT GGTTTCCAGC AAATTATGTG 
AAGCTTTTGA GCCCTGGTAC AAACAAAAGC ACGCCGACAG AACCACCAAA AGCAACATCA 
TTGCCTCCAA CATGCCAAGT CATCGGGATG TACGATTATA TCGCTCAGAA TGACGATGAG 
TTGGCCTTCG GTAAAGGTCA GGTCATTAAC GTGCTGAACA AAGAGGACCC GGACTGGTGG 
AAAGGAGAAC TGAACGGCCA TGTTGGTCTG TTTCCCTCCA ACTATGTGAA ACTGACCACA 
GACATGGATC CCAGTCAGCA ATGGTGTGCA GACCTACATC TCCTGGACAT GCTGAGTCCC 
ACCGAGAGGA AGAGGCAAGG GTACATCCAC GAGCTCATTG TCACCGAGGA GAACTATGTC 
AATGACTTAC AGTTAGTCAC CGAGACATTC CAAAAGCCTC TGCTGGAATC TGAATTACTG 
ACGGAGAAGG AAGTTGCCAT GATTTTTGTC AACTGGAAGG AGCTGATAAT GTGCAATATC 
AAACTGCTCA AGGCCTTGCG AGTTCGCAAG AAAATGTCTG GGGAGAAGAT GCCGGTGAAG 
ATGATCGGGG ACATCCTGAC GGCCCAGTTG CCCCACATGC AGCCGTACAT TCGCTTCTGC 
AGCTGCCAGC TCAACGGCGC CGCTCTCATT CAGCAGAAAA CGGACGAGGT GCCCGAGTTC 
AAGGAGTTTG TGAAAAGGCT TGCAATGGAT CCCCGGTGTA AGGGAATGCC GCTGTCCAGC 
TTCCTACTCA AACCCATGCA GAGAGTAACC AGATACCCGC TCATCATTAA AAATATTATT 
GAAAATACTC CAGAAAATCA CCCTGACCAC AGCCACCTGA AGCAAGCCCT AGAGAAAGCC 
GAAGAATTAT GTTCCCAAGT GAATGAGGGG GTTCGGGAGA AGGAAAACTC AGACCGACTG 
GAGTGGATTC AGGCGCACGT TCAGTGTGAG GGTTTGTCTG AGCAATTAGT ATTTAACTCT 
GTTACAAACT GCCTGGGCCC ACGCAAGTTC CTGCACAGTG GGAAACTGTA CAAAGCCAAG 
AGCAACAAGG AACTATACGG CTTCCTGTTC AACGACTTCC TGCTGCTCAC CCAGATCATT 
AAGCCCCTCG GCTCCTCCGG CAACGATAAG GTGTTCAGCC CCAAGTCCAA TCTCCAGTAC 
AAGATGTACA AAACTCCAAT ATTCCTCAAC GAAGTGCTGG TGAAACTGCC CACGGACCCA 
TCCGGGGATG AGCCAATCTT TCATATTTCT CACATTGACC GAGTCTACAC ATTGCGTGCA 
GAGAGCATCA ATGAGAGGAC GGCCTGGGTG CAGAAAATCA AAGCGGCATC GGAGTTGTAC 
ATAGAGACGG AGAAGAAGAA GAGAGAGAAG GCCTACTTGG TTCGCTCCCA ACGCGCAACA 
GGCATCGGAA GACTGATGGT AAATATCGTT GAGGGAATTG AGTTGAAGCC CTGCAGAACC 
CACGGGAAGA GCAATCCTTA CTGTGAGATC ACCATGGGCT CCCAGTGCCA CATTACCAAG 
ACCATACAGG ACACCCTGAA CCCCAAGTGG AACTCCAACT GCCAATTCTT TATCAAGGAC 
CTGGAGCAGG ACGTTCTGTG CATCACCGTG TTTGAGAGGG ACCAGTTTTC CCCTGACGAT 
TTCCTGGGCC GGACAGAAAT CCGAGTTGCT GACATAAAGA AAGACCAAGG TTCTAAAGGA 
CCAGTAACCA AGTGTCTACT TCTGCACGAA GTTCCTACTG GAGAGATAGT GGTCCGATTG 
GATCTGCAGC TGTTTGACGA GCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_004912128.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912185.1 Xenopus tropicalis intersectin 1 (itsn1), transcript variant X3, mRNA.

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CloneID	OXa06416
Clone ID Related Accession (Same CDS sequence)	XM_004912128.3 , XM_004912128.4
Accession Version	XM_004912128.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intersectin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912128.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGCCACGT TGCCAAAGAG TTCTTCATTC GGCCGCTCAG GTGCCGGCTC CCAGATGAAC 
ACTAAACTTC AGAAAGCTCA GTCCTTTGAT GTCCCAACCC CCCCACCCCT GGCAGAATGG 
GCTGTACCAC AGTCTTCAAG GTTGAAGTAC AGACAGTTGT TCAACAGCCA CGACAAAACC 
ATGAGTGGGC ATTTAACAGG TCCTCAAGCA AGAACTATTC TTATGCAGTC AAGTTTACCG 
CAGAGCCAGC TAGCTACGAT ATGGAACCTT TCAGACATTG ACCTAGATGG AAAACTAACG 
GCCGAAGAAT TTATTTTAGC TATGCACTTA ATAGATGTGG CCATGTCTGG CCAGCCACTT 
CCTCCAGTTC TGCCTCCTGA GTATATTCCC CCATCTTTTA GAAGAGTTCG ATCGGGGAGC 
GGGTTATCGA TTATGAGCTC GGTGTCTGTA GATCAGCGCT TACCGGAGGA ACCAGCAGAA 
GAGGAGCCAC AGAACACAGA CAAAAGACTT CCAGTGACAT TTGAAGACAA GAAAAGGGAA 
AACTTTGAGC GAGGAAACCT GGAATTAGAA AAGAGGAGAC AGGCACTTCT AGAGCAGCAG 
CGCAAAGAGC AGGAGAGGCT GGCACAGCTG GAAAGGGCAG AGCAGGAGAG AAAAGAACGA 
GAGCGGCAAG ATCAAGAGCG CAAGCGGCAG CAAGAGCTGG AAAAACAGCT GGAGAAACAA 
CGAGAGCTGG AGCGGCAACG GGAAGAGGAG CGCAGGAAAG AAATAGAAAG GCGAGAGGCA 
GCAAAGAGAG AACTTGAAAG GCAGAGGCAG TTGGAGTGGG AACGAAACCG GAGGCAAGAA 
CTCCTTAATC AAAGGAACAG AGAGCAAGAG GACATTGTTG TCTTAAAAGC AAAGAAAAAG 
ACTCTTGAGT TTGAGCTGGA AGCTCTAAAT GATAAAAAGC ACCAGCTGGA AGGGAAACTT 
CAGGATATCC GCTGTCGCCT CACTACTCAA CGGCACGAAA TAGAAAGCAC TAACAAATCC 
AGGGAGCTTA GGATCGCAGA GATCACGCAT TTACAGCAGC AGCTTCAGGA ATCTCAGCAG 
CTGCTTGGAA AACTGATTCC TGAGAAACAG TCCCTTAACG ATCAATTAAA GCAGGTTCAA 
CAAAACAGTT TGCACAGAGA TTCCTTGCTG ACACTAAAAA GGGCATTGGA AGCCAAGGAG 
ATGGGCCGCC AGCAGCTCCG AGACCAGTTG GATGAAGTAG AAAAAGAAAC ACGAGCTAAG 
CTCCAGGAAA TCGATGTGTT TAATAACCAA TTAAAGGAAC TGAGAGAATT GTACAACAGA 
CAACAGCTCC AGAAACAGAA GGACTTTGAA GCCGAGAAGA TCAAGCAGAA AGAGCTGGAA 
CGGAAAACCA GTGAGCTGGA CAAGATGAAA GAGGAGGACA AAAGGCGGAT GCTAGAGCAG 
GATAAGCTTT GGCAGGATCG TGTGAAGCAG GAAGAGGAAC GTTACAAATT CCAAGATGAG 
GAGAAGCAGA AAAGGGAAGA ATCCGCTCAA CAATGTGAGG AAGAAAAGAA ACCAGAGATC 
CAAGAGAAGC CCAACAAACT GTTCCACCTA CCACCGGAGC CAGGAAAGCT AGGAGGGCAT 
GTCCCCTGGA TGAATACAGA GAAAGCACCG CTAACATTCA ACCAGGGTGA TGTTAAGGTT 
GTGTATTACC GGGCACTCTA CCCTTTTGAT GCTCGAAGTC ATGATGAAAT CACTATTCAG 
CCTGGGGACA TTATTATGGT GGATGAGAGC CAAACCGGAG AGCCTGGATG GCTGGGAGGT 
GAACTGAAGG GAAAAACTGG CTGGTTTCCT GCCAACTATG CAGAGAGAAT GCCCGAGGGT 
GAATTCCCAA GCACAACTAA ACAGGCAGCA GACACTACGG CCAAACCCAC TGTCCATTTG 
GCACCTTCCA CTCCCACACC TGCAGCTTTT ACAGATAACT CTGCAAATTC CAACAACTGG 
GCAGACTTCA GCTCCACGTG GCCAACCAAT AACACTGATA AAGCAGAGAC GGATAACTGG 
GATACATGGG GAGCTCAACC ATCTCTGACT GTTCCTAGTG CCGGACAGCA CAGGCAGCGC 
TCTGCTTTTA CCCCAGCAAC GGTTACTGGA TCATCTCCAT CTCCTGTACT TGGTCAGGGT 
GAAAAAGTAG AAGGGCTTCA GGCACAAGCC TTGTATCCTT GGAGAGCAAA AAAGGACAAC 
CACCTTAATT TTAACAAAAA TGACATCATC ACGGTTCTAG AGCAGCAGGA TATGTGGTGG 
TTTGGGGAAG TTCAAGGTCA AAAGGGCTGG TTTCCCAAAT CCTACGTAAA ACTTATATCC 
GGGCCACTAA GGAAATCTAC AAGCATCGAT TCCACATCTT CAGAAAGCCC TGCTAGTTTA 
AAAAGAGTCT CTTCACCAGC ATTTAAACCT GCCATGCAAG GAGAAGAATA CATAGCCATG 
TACACGTACG AGAGCAACGA GCAGGGAGAC TTGACCTTTC AGCAAGGAGA TCTGATTGTG 
GTGATTAAAA AAGATGGGGA CTGGTGGACT GGCACGGTGG GAGAGAAGAC GGGGGTTTTC 
CCTTCCAACT ACGTAAGGCC CAAAGACTCC GAGGCGGCTG GAAGCGCAGG AAAGACAGGA 
AGCCTTGGGA AGAAACCAGA AATTGCCCAG GTTATTGCTT CTTACACAGC AACTGGCCCT 
GAGCAGCTGA CACTGGCTCC TGGCCAACTT ATCCTCATCC GGAAAAAGAA TCCTGGAGGA 
TGGTGGGAAG GAGAACTGCA GGCACGTGGG AAGAAGCGAC AGATTGGATG GTTTCCAGCA 
AATTATGTGA AGCTTTTGAG CCCTGGTACA AACAAAAGCA CGCCGACAGA ACCACCAAAA 
GCAACATCAT TGCCTCCAAC ATGCCAAGTC ATCGGGATGT ACGATTATAT CGCTCAGAAT 
GACGATGAGT TGGCCTTCGG TAAAGGTCAG GTCATTAACG TGCTGAACAA AGAGGACCCG 
GACTGGTGGA AAGGAGAACT GAACGGCCAT GTTGGTCTGT TTCCCTCCAA CTATGTGAAA 
CTGACCACAG ACATGGATCC CAGTCAGCAA TGGTGTGCAG ACCTACATCT CCTGGACATG 
CTGAGTCCCA CCGAGAGGAA GAGGCAAGGG TACATCCACG AGCTCATTGT CACCGAGGAG 
AACTATGTCA ATGACTTACA GTTAGTCACC GAGACATTCC AAAAGCCTCT GCTGGAATCT 
GAATTACTGA CGGAGAAGGA AGTTGCCATG ATTTTTGTCA ACTGGAAGGA GCTGATAATG 
TGCAATATCA AACTGCTCAA GGCCTTGCGA GTTCGCAAGA AAATGTCTGG GGAGAAGATG 
CCGGTGAAGA TGATCGGGGA CATCCTGACG GCCCAGTTGC CCCACATGCA GCCGTACATT 
CGCTTCTGCA GCTGCCAGCT CAACGGCGCC GCTCTCATTC AGCAGAAAAC GGACGAGGTG 
CCCGAGTTCA AGGAGTTTGT GAAAAGGCTT GCAATGGATC CCCGGTGTAA GGGAATGCCG 
CTGTCCAGCT TCCTACTCAA ACCCATGCAG AGAGTAACCA GATACCCGCT CATCATTAAA 
AATATTATTG AAAATACTCC AGAAAATCAC CCTGACCACA GCCACCTGAA GCAAGCCCTA 
GAGAAAGCCG AAGAATTATG TTCCCAAGTG AATGAGGGGG TTCGGGAGAA GGAAAACTCA 
GACCGACTGG AGTGGATTCA GGCGCACGTT CAGTGTGAGG GTTTGTCTGA GCAATTAGTA 
TTTAACTCTG TTACAAACTG CCTGGGCCCA CGCAAGTTCC TGCACAGTGG GAAACTGTAC 
AAAGCCAAGA GCAACAAGGA ACTATACGGC TTCCTGTTCA ACGACTTCCT GCTGCTCACC 
CAGATCATTA AGCCCCTCGG CTCCTCCGGC AACGATAAGG TGTTCAGCCC CAAGTCCAAT 
CTCCAGTACA AGATGTACAA AACTCCAATA TTCCTCAACG AAGTGCTGGT GAAACTGCCC 
ACGGACCCAT CCGGGGATGA GCCAATCTTT CATATTTCTC ACATTGACCG AGTCTACACA 
TTGCGTGCAG AGAGCATCAA TGAGAGGACG GCCTGGGTGC AGAAAATCAA AGCGGCATCG 
GAGTTGTACA TAGAGACGGA GAAGAAGAAG AGAGAGAAGG CCTACTTGGT TCGCTCCCAA 
CGCGCAACAG GCATCGGAAG ACTGATGGTA AATATCGTTG AGGGAATTGA GTTGAAGCCC 
TGCAGAACCC ACGGGAAGAG CAATCCTTAC TGTGAGATCA CCATGGGCTC CCAGTGCCAC 
ATTACCAAGA CCATACAGGA CACCCTGAAC CCCAAGTGGA ACTCCAACTG CCAATTCTTT 
ATCAAGGACC TGGAGCAGGA CGTTCTGTGC ATCACCGTGT TTGAGAGGGA CCAGTTTTCC 
CCTGACGATT TCCTGGGCCG GACAGAAATC CGAGTTGCTG ACATAAAGAA AGACCAAGGT 
TCTAAAGGAC CAGTAACCAA GTGTCTACTT CTGCACGAAG TTCCTACTGG AGAGATAGTG 
GTCCGATTGG ATCTGCAGCT GTTTGACGAG CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912185.1
CDS	301..5256
Translation	MAQFGTPFGGNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSAAAAAAAAAGFATLPKSSSFGRSGAGSQMNTKLQKAQSFDVPTPPPLAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGLSIMSSVSVDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRELRIAEITHLQQQLQESQQLLGKLIPEKQSLNDQLKQVQQNSLHRDSLLTLKRALEAKEMGRQQLRDQLDEVEKETRAKLQEIDVFNNQLKELRELYNRQQLQKQKDFEAEKIKQKELERKTSELDKMKEEDKRRMLEQDKLWQDRVKQEEERYKFQDEEKQKREESAQQCEEEKKPEIQEKPNKLFHLPPEPGKLGGHVPWMNTEKAPLTFNQGDVKVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEPGWLGGELKGKTGWFPANYAERMPEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTWPTNNTDKAETDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVTGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRVSSPAFKPAMQGEEYIAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTNKSTPTEPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGELNGHVGLFPSNYVKLTTDMDPSQQWCADLHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_004912128.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912128.3

ORF Insert Sequence:

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGCCACGT TGCCAAAGAG TTCTTCATTC GGCCGCTCAG GTGCCGGCTC CCAGATGAAC 
ACTAAACTTC AGAAAGCTCA GTCCTTTGAT GTCCCAACCC CCCCACCCCT GGCAGAATGG 
GCTGTACCAC AGTCTTCAAG GTTGAAGTAC AGACAGTTGT TCAACAGCCA CGACAAAACC 
ATGAGTGGGC ATTTAACAGG TCCTCAAGCA AGAACTATTC TTATGCAGTC AAGTTTACCG 
CAGAGCCAGC TAGCTACGAT ATGGAACCTT TCAGACATTG ACCTAGATGG AAAACTAACG 
GCCGAAGAAT TTATTTTAGC TATGCACTTA ATAGATGTGG CCATGTCTGG CCAGCCACTT 
CCTCCAGTTC TGCCTCCTGA GTATATTCCC CCATCTTTTA GAAGAGTTCG ATCGGGGAGC 
GGGTTATCGA TTATGAGCTC GGTGTCTGTA GATCAGCGCT TACCGGAGGA ACCAGCAGAA 
GAGGAGCCAC AGAACACAGA CAAAAGACTT CCAGTGACAT TTGAAGACAA GAAAAGGGAA 
AACTTTGAGC GAGGAAACCT GGAATTAGAA AAGAGGAGAC AGGCACTTCT AGAGCAGCAG 
CGCAAAGAGC AGGAGAGGCT GGCACAGCTG GAAAGGGCAG AGCAGGAGAG AAAAGAACGA 
GAGCGGCAAG ATCAAGAGCG CAAGCGGCAG CAAGAGCTGG AAAAACAGCT GGAGAAACAA 
CGAGAGCTGG AGCGGCAACG GGAAGAGGAG CGCAGGAAAG AAATAGAAAG GCGAGAGGCA 
GCAAAGAGAG AACTTGAAAG GCAGAGGCAG TTGGAGTGGG AACGAAACCG GAGGCAAGAA 
CTCCTTAATC AAAGGAACAG AGAGCAAGAG GACATTGTTG TCTTAAAAGC AAAGAAAAAG 
ACTCTTGAGT TTGAGCTGGA AGCTCTAAAT GATAAAAAGC ACCAGCTGGA AGGGAAACTT 
CAGGATATCC GCTGTCGCCT CACTACTCAA CGGCACGAAA TAGAAAGCAC TAACAAATCC 
AGGGAGCTTA GGATCGCAGA GATCACGCAT TTACAGCAGC AGCTTCAGGA ATCTCAGCAG 
CTGCTTGGAA AACTGATTCC TGAGAAACAG TCCCTTAACG ATCAATTAAA GCAGGTTCAA 
CAAAACAGTT TGCACAGAGA TTCCTTGCTG ACACTAAAAA GGGCATTGGA AGCCAAGGAG 
ATGGGCCGCC AGCAGCTCCG AGACCAGTTG GATGAAGTAG AAAAAGAAAC ACGAGCTAAG 
CTCCAGGAAA TCGATGTGTT TAATAACCAA TTAAAGGAAC TGAGAGAATT GTACAACAGA 
CAACAGCTCC AGAAACAGAA GGACTTTGAA GCCGAGAAGA TCAAGCAGAA AGAGCTGGAA 
CGGAAAACCA GTGAGCTGGA CAAGATGAAA GAGGAGGACA AAAGGCGGAT GCTAGAGCAG 
GATAAGCTTT GGCAGGATCG TGTGAAGCAG GAAGAGGAAC GTTACAAATT CCAAGATGAG 
GAGAAGCAGA AAAGGGAAGA ATCCGCTCAA CAATGTGAGG AAGAAAAGAA ACCAGAGATC 
CAAGAGAAGC CCAACAAACT GTTCCACCTA CCACCGGAGC CAGGAAAGCT AGGAGGGCAT 
GTCCCCTGGA TGAATACAGA GAAAGCACCG CTAACATTCA ACCAGGGTGA TGTTAAGGTT 
GTGTATTACC GGGCACTCTA CCCTTTTGAT GCTCGAAGTC ATGATGAAAT CACTATTCAG 
CCTGGGGACA TTATTATGGT GGATGAGAGC CAAACCGGAG AGCCTGGATG GCTGGGAGGT 
GAACTGAAGG GAAAAACTGG CTGGTTTCCT GCCAACTATG CAGAGAGAAT GCCCGAGGGT 
GAATTCCCAA GCACAACTAA ACAGGCAGCA GACACTACGG CCAAACCCAC TGTCCATTTG 
GCACCTTCCA CTCCCACACC TGCAGCTTTT ACAGATAACT CTGCAAATTC CAACAACTGG 
GCAGACTTCA GCTCCACGTG GCCAACCAAT AACACTGATA AAGCAGAGAC GGATAACTGG 
GATACATGGG GAGCTCAACC ATCTCTGACT GTTCCTAGTG CCGGACAGCA CAGGCAGCGC 
TCTGCTTTTA CCCCAGCAAC GGTTACTGGA TCATCTCCAT CTCCTGTACT TGGTCAGGGT 
GAAAAAGTAG AAGGGCTTCA GGCACAAGCC TTGTATCCTT GGAGAGCAAA AAAGGACAAC 
CACCTTAATT TTAACAAAAA TGACATCATC ACGGTTCTAG AGCAGCAGGA TATGTGGTGG 
TTTGGGGAAG TTCAAGGTCA AAAGGGCTGG TTTCCCAAAT CCTACGTAAA ACTTATATCC 
GGGCCACTAA GGAAATCTAC AAGCATCGAT TCCACATCTT CAGAAAGCCC TGCTAGTTTA 
AAAAGAGTCT CTTCACCAGC ATTTAAACCT GCCATGCAAG GAGAAGAATA CATAGCCATG 
TACACGTACG AGAGCAACGA GCAGGGAGAC TTGACCTTTC AGCAAGGAGA TCTGATTGTG 
GTGATTAAAA AAGATGGGGA CTGGTGGACT GGCACGGTGG GAGAGAAGAC GGGGGTTTTC 
CCTTCCAACT ACGTAAGGCC CAAAGACTCC GAGGCGGCTG GAAGCGCAGG AAAGACAGGA 
AGCCTTGGGA AGAAACCAGA AATTGCCCAG GTTATTGCTT CTTACACAGC AACTGGCCCT 
GAGCAGCTGA CACTGGCTCC TGGCCAACTT ATCCTCATCC GGAAAAAGAA TCCTGGAGGA 
TGGTGGGAAG GAGAACTGCA GGCACGTGGG AAGAAGCGAC AGATTGGATG GTTTCCAGCA 
AATTATGTGA AGCTTTTGAG CCCTGGTACA AACAAAAGCA CGCCGACAGA ACCACCAAAA 
GCAACATCAT TGCCTCCAAC ATGCCAAGTC ATCGGGATGT ACGATTATAT CGCTCAGAAT 
GACGATGAGT TGGCCTTCGG TAAAGGTCAG GTCATTAACG TGCTGAACAA AGAGGACCCG 
GACTGGTGGA AAGGAGAACT GAACGGCCAT GTTGGTCTGT TTCCCTCCAA CTATGTGAAA 
CTGACCACAG ACATGGATCC CAGTCAGCAA TGGTGTGCAG ACCTACATCT CCTGGACATG 
CTGAGTCCCA CCGAGAGGAA GAGGCAAGGG TACATCCACG AGCTCATTGT CACCGAGGAG 
AACTATGTCA ATGACTTACA GTTAGTCACC GAGACATTCC AAAAGCCTCT GCTGGAATCT 
GAATTACTGA CGGAGAAGGA AGTTGCCATG ATTTTTGTCA ACTGGAAGGA GCTGATAATG 
TGCAATATCA AACTGCTCAA GGCCTTGCGA GTTCGCAAGA AAATGTCTGG GGAGAAGATG 
CCGGTGAAGA TGATCGGGGA CATCCTGACG GCCCAGTTGC CCCACATGCA GCCGTACATT 
CGCTTCTGCA GCTGCCAGCT CAACGGCGCC GCTCTCATTC AGCAGAAAAC GGACGAGGTG 
CCCGAGTTCA AGGAGTTTGT GAAAAGGCTT GCAATGGATC CCCGGTGTAA GGGAATGCCG 
CTGTCCAGCT TCCTACTCAA ACCCATGCAG AGAGTAACCA GATACCCGCT CATCATTAAA 
AATATTATTG AAAATACTCC AGAAAATCAC CCTGACCACA GCCACCTGAA GCAAGCCCTA 
GAGAAAGCCG AAGAATTATG TTCCCAAGTG AATGAGGGGG TTCGGGAGAA GGAAAACTCA 
GACCGACTGG AGTGGATTCA GGCGCACGTT CAGTGTGAGG GTTTGTCTGA GCAATTAGTA 
TTTAACTCTG TTACAAACTG CCTGGGCCCA CGCAAGTTCC TGCACAGTGG GAAACTGTAC 
AAAGCCAAGA GCAACAAGGA ACTATACGGC TTCCTGTTCA ACGACTTCCT GCTGCTCACC 
CAGATCATTA AGCCCCTCGG CTCCTCCGGC AACGATAAGG TGTTCAGCCC CAAGTCCAAT 
CTCCAGTACA AGATGTACAA AACTCCAATA TTCCTCAACG AAGTGCTGGT GAAACTGCCC 
ACGGACCCAT CCGGGGATGA GCCAATCTTT CATATTTCTC ACATTGACCG AGTCTACACA 
TTGCGTGCAG AGAGCATCAA TGAGAGGACG GCCTGGGTGC AGAAAATCAA AGCGGCATCG 
GAGTTGTACA TAGAGACGGA GAAGAAGAAG AGAGAGAAGG CCTACTTGGT TCGCTCCCAA 
CGCGCAACAG GCATCGGAAG ACTGATGGTA AATATCGTTG AGGGAATTGA GTTGAAGCCC 
TGCAGAACCC ACGGGAAGAG CAATCCTTAC TGTGAGATCA CCATGGGCTC CCAGTGCCAC 
ATTACCAAGA CCATACAGGA CACCCTGAAC CCCAAGTGGA ACTCCAACTG CCAATTCTTT 
ATCAAGGACC TGGAGCAGGA CGTTCTGTGC ATCACCGTGT TTGAGAGGGA CCAGTTTTCC 
CCTGACGATT TCCTGGGCCG GACAGAAATC CGAGTTGCTG ACATAAAGAA AGACCAAGGT 
TCTAAAGGAC CAGTAACCAA GTGTCTACTT CTGCACGAAG TTCCTACTGG AGAGATAGTG 
GTCCGATTGG ATCTGCAGCT GTTTGACGAG CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itsn1 ( XM_004912129.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912186.1 Xenopus tropicalis intersectin 1 (itsn1), transcript variant X4, mRNA.

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CloneID	OXa06417
Clone ID Related Accession (Same CDS sequence)	XM_004912129.3 , XM_004912129.4
Accession Version	XM_004912129.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intersectin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912129.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGCCACGT TGCCAAAGAG TTCTTCATTC GGCCGCTCAG GTGCCGGCTC CCAGATGAAC 
ACTAAACTTC AGAAAGCTCA GTCCTTTGAT GTCCCAACCC CCCCACCCCT GGCAGAATGG 
GCTGTACCAC AGTCTTCAAG GTTGAAGTAC AGACAGTTGT TCAACAGCCA CGACAAAACC 
ATGAGTGGGC ATTTAACAGG TCCTCAAGCA AGAACTATTC TTATGCAGTC AAGTTTACCG 
CAGAGCCAGC TAGCTACGAT ATGGAACCTT TCAGACATTG ACCTAGATGG AAAACTAACG 
GCCGAAGAAT TTATTTTAGC TATGCACTTA ATAGATGTGG CCATGTCTGG CCAGCCACTT 
CCTCCAGTTC TGCCTCCTGA GTATATTCCC CCATCTTTTA GAGTTCGATC GGGGAGCGGG 
TTATCGATTA TGAGCTCGGT GTCTGTAGAT CAGCGCTTAC CGGAGGAACC AGCAGAAGAG 
GAGCCACAGA ACACAGACAA AAGACTTCCA GTGACATTTG AAGACAAGAA AAGGGAAAAC 
TTTGAGCGAG GAAACCTGGA ATTAGAAAAG AGGAGACAGG CACTTCTAGA GCAGCAGCGC 
AAAGAGCAGG AGAGGCTGGC ACAGCTGGAA AGGGCAGAGC AGGAGAGAAA AGAACGAGAG 
CGGCAAGATC AAGAGCGCAA GCGGCAGCAA GAGCTGGAAA AACAGCTGGA GAAACAACGA 
GAGCTGGAGC GGCAACGGGA AGAGGAGCGC AGGAAAGAAA TAGAAAGGCG AGAGGCAGCA 
AAGAGAGAAC TTGAAAGGCA GAGGCAGTTG GAGTGGGAAC GAAACCGGAG GCAAGAACTC 
CTTAATCAAA GGAACAGAGA GCAAGAGGAC ATTGTTGTCT TAAAAGCAAA GAAAAAGACT 
CTTGAGTTTG AGCTGGAAGC TCTAAATGAT AAAAAGCACC AGCTGGAAGG GAAACTTCAG 
GATATCCGCT GTCGCCTCAC TACTCAACGG CACGAAATAG AAAGCACTAA CAAATCCAGG 
GAGCTTAGGA TCGCAGAGAT CACGCATTTA CAGCAGCAGC TTCAGGAATC TCAGCAGCTG 
CTTGGAAAAC TGATTCCTGA GAAACAGTCC CTTAACGATC AATTAAAGCA GGTTCAACAA 
AACAGTTTGC ACAGAGATTC CTTGCTGACA CTAAAAAGGG CATTGGAAGC CAAGGAGATG 
GGCCGCCAGC AGCTCCGAGA CCAGTTGGAT GAAGTAGAAA AAGAAACACG AGCTAAGCTC 
CAGGAAATCG ATGTGTTTAA TAACCAATTA AAGGAACTGA GAGAATTGTA CAACAGACAA 
CAGCTCCAGA AACAGAAGGA CTTTGAAGCC GAGAAGATCA AGCAGAAAGA GCTGGAACGG 
AAAACCAGTG AGCTGGACAA GATGAAAGAG GAGGACAAAA GGCGGATGCT AGAGCAGGAT 
AAGCTTTGGC AGGATCGTGT GAAGCAGGAA GAGGAACGTT ACAAATTCCA AGATGAGGAG 
AAGCAGAAAA GGGAAGAATC CGCTCAACAA TGTGAGGAAG AAAAGAAACC AGAGATCCAA 
GAGAAGCCCA ACAAACTGTT CCACCTACCA CCGGAGCCAG GAAAGCTAGG AGGGCATGTC 
CCCTGGATGA ATACAGAGAA AGCACCGCTA ACATTCAACC AGGGTGATGT TAAGGTTGTG 
TATTACCGGG CACTCTACCC TTTTGATGCT CGAAGTCATG ATGAAATCAC TATTCAGCCT 
GGGGACATTA TTATGGTGGA TGAGAGCCAA ACCGGAGAGC CTGGATGGCT GGGAGGTGAA 
CTGAAGGGAA AAACTGGCTG GTTTCCTGCC AACTATGCAG AGAGAATGCC CGAGGGTGAA 
TTCCCAAGCA CAACTAAACA GGCAGCAGAC ACTACGGCCA AACCCACTGT CCATTTGGCA 
CCTTCCACTC CCACACCTGC AGCTTTTACA GATAACTCTG CAAATTCCAA CAACTGGGCA 
GACTTCAGCT CCACGTGGCC AACCAATAAC ACTGATAAAG CAGAGACGGA TAACTGGGAT 
ACATGGGGAG CTCAACCATC TCTGACTGTT CCTAGTGCCG GACAGCACAG GCAGCGCTCT 
GCTTTTACCC CAGCAACGGT TACTGGATCA TCTCCATCTC CTGTACTTGG TCAGGGTGAA 
AAAGTAGAAG GGCTTCAGGC ACAAGCCTTG TATCCTTGGA GAGCAAAAAA GGACAACCAC 
CTTAATTTTA ACAAAAATGA CATCATCACG GTTCTAGAGC AGCAGGATAT GTGGTGGTTT 
GGGGAAGTTC AAGGTCAAAA GGGCTGGTTT CCCAAATCCT ACGTAAAACT TATATCCGGG 
CCACTAAGGA AATCTACAAG CATCGATTCC ACATCTTCAG AAAGCCCTGC TAGTTTAAAA 
AGAGTCTCTT CACCAGCATT TAAACCTGCC ATGCAAGGAG AAGAATACAT AGCCATGTAC 
ACGTACGAGA GCAACGAGCA GGGAGACTTG ACCTTTCAGC AAGGAGATCT GATTGTGGTG 
ATTAAAAAAG ATGGGGACTG GTGGACTGGC ACGGTGGGAG AGAAGACGGG GGTTTTCCCT 
TCCAACTACG TAAGGCCCAA AGACTCCGAG GCGGCTGGAA GCGCAGGAAA GACAGGAAGC 
CTTGGGAAGA AACCAGAAAT TGCCCAGGTT ATTGCTTCTT ACACAGCAAC TGGCCCTGAG 
CAGCTGACAC TGGCTCCTGG CCAACTTATC CTCATCCGGA AAAAGAATCC TGGAGGATGG 
TGGGAAGGAG AACTGCAGGC ACGTGGGAAG AAGCGACAGA TTGGATGGTT TCCAGCAAAT 
TATGTGAAGC TTTTGAGCCC TGGTACAAAC AAAAGCACGC CGACAGAACC ACCAAAAGCA 
ACATCATTGC CTCCAACATG CCAAGTCATC GGGATGTACG ATTATATCGC TCAGAATGAC 
GATGAGTTGG CCTTCGGTAA AGGTCAGGTC ATTAACGTGC TGAACAAAGA GGACCCGGAC 
TGGTGGAAAG GAGAACTGAA CGGCCATGTT GGTCTGTTTC CCTCCAACTA TGTGAAACTG 
ACCACAGACA TGGATCCCAG TCAGCAATGG TGTGCAGACC TACATCTCCT GGACATGCTG 
AGTCCCACCG AGAGGAAGAG GCAAGGGTAC ATCCACGAGC TCATTGTCAC CGAGGAGAAC 
TATGTCAATG ACTTACAGTT AGTCACCGAG ACATTCCAAA AGCCTCTGCT GGAATCTGAA 
TTACTGACGG AGAAGGAAGT TGCCATGATT TTTGTCAACT GGAAGGAGCT GATAATGTGC 
AATATCAAAC TGCTCAAGGC CTTGCGAGTT CGCAAGAAAA TGTCTGGGGA GAAGATGCCG 
GTGAAGATGA TCGGGGACAT CCTGACGGCC CAGTTGCCCC ACATGCAGCC GTACATTCGC 
TTCTGCAGCT GCCAGCTCAA CGGCGCCGCT CTCATTCAGC AGAAAACGGA CGAGGTGCCC 
GAGTTCAAGG AGTTTGTGAA AAGGCTTGCA ATGGATCCCC GGTGTAAGGG AATGCCGCTG 
TCCAGCTTCC TACTCAAACC CATGCAGAGA GTAACCAGAT ACCCGCTCAT CATTAAAAAT 
ATTATTGAAA ATACTCCAGA AAATCACCCT GACCACAGCC ACCTGAAGCA AGCCCTAGAG 
AAAGCCGAAG AATTATGTTC CCAAGTGAAT GAGGGGGTTC GGGAGAAGGA AAACTCAGAC 
CGACTGGAGT GGATTCAGGC GCACGTTCAG TGTGAGGGTT TGTCTGAGCA ATTAGTATTT 
AACTCTGTTA CAAACTGCCT GGGCCCACGC AAGTTCCTGC ACAGTGGGAA ACTGTACAAA 
GCCAAGAGCA ACAAGGAACT ATACGGCTTC CTGTTCAACG ACTTCCTGCT GCTCACCCAG 
ATCATTAAGC CCCTCGGCTC CTCCGGCAAC GATAAGGTGT TCAGCCCCAA GTCCAATCTC 
CAGTACAAGA TGTACAAAAC TCCAATATTC CTCAACGAAG TGCTGGTGAA ACTGCCCACG 
GACCCATCCG GGGATGAGCC AATCTTTCAT ATTTCTCACA TTGACCGAGT CTACACATTG 
CGTGCAGAGA GCATCAATGA GAGGACGGCC TGGGTGCAGA AAATCAAAGC GGCATCGGAG 
TTGTACATAG AGACGGAGAA GAAGAAGAGA GAGAAGGCCT ACTTGGTTCG CTCCCAACGC 
GCAACAGGCA TCGGAAGACT GATGGTAAAT ATCGTTGAGG GAATTGAGTT GAAGCCCTGC 
AGAACCCACG GGAAGAGCAA TCCTTACTGT GAGATCACCA TGGGCTCCCA GTGCCACATT 
ACCAAGACCA TACAGGACAC CCTGAACCCC AAGTGGAACT CCAACTGCCA ATTCTTTATC 
AAGGACCTGG AGCAGGACGT TCTGTGCATC ACCGTGTTTG AGAGGGACCA GTTTTCCCCT 
GACGATTTCC TGGGCCGGAC AGAAATCCGA GTTGCTGACA TAAAGAAAGA CCAAGGTTCT 
AAAGGACCAG TAACCAAGTG TCTACTTCTG CACGAAGTTC CTACTGGAGA GATAGTGGTC 
CGATTGGATC TGCAGCTGTT TGACGAGCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912186.1
CDS	301..5253
Translation	MAQFGTPFGGNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSAAAAAAAAAGFATLPKSSSFGRSGAGSQMNTKLQKAQSFDVPTPPPLAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRVRSGSGLSIMSSVSVDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRELRIAEITHLQQQLQESQQLLGKLIPEKQSLNDQLKQVQQNSLHRDSLLTLKRALEAKEMGRQQLRDQLDEVEKETRAKLQEIDVFNNQLKELRELYNRQQLQKQKDFEAEKIKQKELERKTSELDKMKEEDKRRMLEQDKLWQDRVKQEEERYKFQDEEKQKREESAQQCEEEKKPEIQEKPNKLFHLPPEPGKLGGHVPWMNTEKAPLTFNQGDVKVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEPGWLGGELKGKTGWFPANYAERMPEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTWPTNNTDKAETDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVTGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRVSSPAFKPAMQGEEYIAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTNKSTPTEPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGELNGHVGLFPSNYVKLTTDMDPSQQWCADLHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_004912129.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intersectin 1 (itsn1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912129.3

ORF Insert Sequence:

ATGGCTCAGT TTGGAACTCC TTTTGGGGGT AATTTGGACA TCTGGGCCAT AACAGTGGAG 
GAGCGAGCTA AACATGACCA GCAGTTCCAT GGGCTCCAGC CAACAGCTGG ATATATCACT 
GGTGACCAAG CTAGGAATTT TTTCCTTCAG TCCGGCCTCC CCCAGCCTGT GCTGGCACAG 
ATATGGGCCC TGGCTGACAT GAACAACGAT GGGAGGATGG ACCAACTGGA GTTCTCCATA 
GCTATGAAAT TGATCAAGCT AAAACTACAA GGTTACCCGC TGCCCTCTGC TCTCCCTTCC 
AATATGTTGA AGCAACCAGT TGCCATGTCC GCTGCTGCCG CCGCTGCTGC CGCTGCGGGG 
TTTGCCACGT TGCCAAAGAG TTCTTCATTC GGCCGCTCAG GTGCCGGCTC CCAGATGAAC 
ACTAAACTTC AGAAAGCTCA GTCCTTTGAT GTCCCAACCC CCCCACCCCT GGCAGAATGG 
GCTGTACCAC AGTCTTCAAG GTTGAAGTAC AGACAGTTGT TCAACAGCCA CGACAAAACC 
ATGAGTGGGC ATTTAACAGG TCCTCAAGCA AGAACTATTC TTATGCAGTC AAGTTTACCG 
CAGAGCCAGC TAGCTACGAT ATGGAACCTT TCAGACATTG ACCTAGATGG AAAACTAACG 
GCCGAAGAAT TTATTTTAGC TATGCACTTA ATAGATGTGG CCATGTCTGG CCAGCCACTT 
CCTCCAGTTC TGCCTCCTGA GTATATTCCC CCATCTTTTA GAGTTCGATC GGGGAGCGGG 
TTATCGATTA TGAGCTCGGT GTCTGTAGAT CAGCGCTTAC CGGAGGAACC AGCAGAAGAG 
GAGCCACAGA ACACAGACAA AAGACTTCCA GTGACATTTG AAGACAAGAA AAGGGAAAAC 
TTTGAGCGAG GAAACCTGGA ATTAGAAAAG AGGAGACAGG CACTTCTAGA GCAGCAGCGC 
AAAGAGCAGG AGAGGCTGGC ACAGCTGGAA AGGGCAGAGC AGGAGAGAAA AGAACGAGAG 
CGGCAAGATC AAGAGCGCAA GCGGCAGCAA GAGCTGGAAA AACAGCTGGA GAAACAACGA 
GAGCTGGAGC GGCAACGGGA AGAGGAGCGC AGGAAAGAAA TAGAAAGGCG AGAGGCAGCA 
AAGAGAGAAC TTGAAAGGCA GAGGCAGTTG GAGTGGGAAC GAAACCGGAG GCAAGAACTC 
CTTAATCAAA GGAACAGAGA GCAAGAGGAC ATTGTTGTCT TAAAAGCAAA GAAAAAGACT 
CTTGAGTTTG AGCTGGAAGC TCTAAATGAT AAAAAGCACC AGCTGGAAGG GAAACTTCAG 
GATATCCGCT GTCGCCTCAC TACTCAACGG CACGAAATAG AAAGCACTAA CAAATCCAGG 
GAGCTTAGGA TCGCAGAGAT CACGCATTTA CAGCAGCAGC TTCAGGAATC TCAGCAGCTG 
CTTGGAAAAC TGATTCCTGA GAAACAGTCC CTTAACGATC AATTAAAGCA GGTTCAACAA 
AACAGTTTGC ACAGAGATTC CTTGCTGACA CTAAAAAGGG CATTGGAAGC CAAGGAGATG 
GGCCGCCAGC AGCTCCGAGA CCAGTTGGAT GAAGTAGAAA AAGAAACACG AGCTAAGCTC 
CAGGAAATCG ATGTGTTTAA TAACCAATTA AAGGAACTGA GAGAATTGTA CAACAGACAA 
CAGCTCCAGA AACAGAAGGA CTTTGAAGCC GAGAAGATCA AGCAGAAAGA GCTGGAACGG 
AAAACCAGTG AGCTGGACAA GATGAAAGAG GAGGACAAAA GGCGGATGCT AGAGCAGGAT 
AAGCTTTGGC AGGATCGTGT GAAGCAGGAA GAGGAACGTT ACAAATTCCA AGATGAGGAG 
AAGCAGAAAA GGGAAGAATC CGCTCAACAA TGTGAGGAAG AAAAGAAACC AGAGATCCAA 
GAGAAGCCCA ACAAACTGTT CCACCTACCA CCGGAGCCAG GAAAGCTAGG AGGGCATGTC 
CCCTGGATGA ATACAGAGAA AGCACCGCTA ACATTCAACC AGGGTGATGT TAAGGTTGTG 
TATTACCGGG CACTCTACCC TTTTGATGCT CGAAGTCATG ATGAAATCAC TATTCAGCCT 
GGGGACATTA TTATGGTGGA TGAGAGCCAA ACCGGAGAGC CTGGATGGCT GGGAGGTGAA 
CTGAAGGGAA AAACTGGCTG GTTTCCTGCC AACTATGCAG AGAGAATGCC CGAGGGTGAA 
TTCCCAAGCA CAACTAAACA GGCAGCAGAC ACTACGGCCA AACCCACTGT CCATTTGGCA 
CCTTCCACTC CCACACCTGC AGCTTTTACA GATAACTCTG CAAATTCCAA CAACTGGGCA 
GACTTCAGCT CCACGTGGCC AACCAATAAC ACTGATAAAG CAGAGACGGA TAACTGGGAT 
ACATGGGGAG CTCAACCATC TCTGACTGTT CCTAGTGCCG GACAGCACAG GCAGCGCTCT 
GCTTTTACCC CAGCAACGGT TACTGGATCA TCTCCATCTC CTGTACTTGG TCAGGGTGAA 
AAAGTAGAAG GGCTTCAGGC ACAAGCCTTG TATCCTTGGA GAGCAAAAAA GGACAACCAC 
CTTAATTTTA ACAAAAATGA CATCATCACG GTTCTAGAGC AGCAGGATAT GTGGTGGTTT 
GGGGAAGTTC AAGGTCAAAA GGGCTGGTTT CCCAAATCCT ACGTAAAACT TATATCCGGG 
CCACTAAGGA AATCTACAAG CATCGATTCC ACATCTTCAG AAAGCCCTGC TAGTTTAAAA 
AGAGTCTCTT CACCAGCATT TAAACCTGCC ATGCAAGGAG AAGAATACAT AGCCATGTAC 
ACGTACGAGA GCAACGAGCA GGGAGACTTG ACCTTTCAGC AAGGAGATCT GATTGTGGTG 
ATTAAAAAAG ATGGGGACTG GTGGACTGGC ACGGTGGGAG AGAAGACGGG GGTTTTCCCT 
TCCAACTACG TAAGGCCCAA AGACTCCGAG GCGGCTGGAA GCGCAGGAAA GACAGGAAGC 
CTTGGGAAGA AACCAGAAAT TGCCCAGGTT ATTGCTTCTT ACACAGCAAC TGGCCCTGAG 
CAGCTGACAC TGGCTCCTGG CCAACTTATC CTCATCCGGA AAAAGAATCC TGGAGGATGG 
TGGGAAGGAG AACTGCAGGC ACGTGGGAAG AAGCGACAGA TTGGATGGTT TCCAGCAAAT 
TATGTGAAGC TTTTGAGCCC TGGTACAAAC AAAAGCACGC CGACAGAACC ACCAAAAGCA 
ACATCATTGC CTCCAACATG CCAAGTCATC GGGATGTACG ATTATATCGC TCAGAATGAC 
GATGAGTTGG CCTTCGGTAA AGGTCAGGTC ATTAACGTGC TGAACAAAGA GGACCCGGAC 
TGGTGGAAAG GAGAACTGAA CGGCCATGTT GGTCTGTTTC CCTCCAACTA TGTGAAACTG 
ACCACAGACA TGGATCCCAG TCAGCAATGG TGTGCAGACC TACATCTCCT GGACATGCTG 
AGTCCCACCG AGAGGAAGAG GCAAGGGTAC ATCCACGAGC TCATTGTCAC CGAGGAGAAC 
TATGTCAATG ACTTACAGTT AGTCACCGAG ACATTCCAAA AGCCTCTGCT GGAATCTGAA 
TTACTGACGG AGAAGGAAGT TGCCATGATT TTTGTCAACT GGAAGGAGCT GATAATGTGC 
AATATCAAAC TGCTCAAGGC CTTGCGAGTT CGCAAGAAAA TGTCTGGGGA GAAGATGCCG 
GTGAAGATGA TCGGGGACAT CCTGACGGCC CAGTTGCCCC ACATGCAGCC GTACATTCGC 
TTCTGCAGCT GCCAGCTCAA CGGCGCCGCT CTCATTCAGC AGAAAACGGA CGAGGTGCCC 
GAGTTCAAGG AGTTTGTGAA AAGGCTTGCA ATGGATCCCC GGTGTAAGGG AATGCCGCTG 
TCCAGCTTCC TACTCAAACC CATGCAGAGA GTAACCAGAT ACCCGCTCAT CATTAAAAAT 
ATTATTGAAA ATACTCCAGA AAATCACCCT GACCACAGCC ACCTGAAGCA AGCCCTAGAG 
AAAGCCGAAG AATTATGTTC CCAAGTGAAT GAGGGGGTTC GGGAGAAGGA AAACTCAGAC 
CGACTGGAGT GGATTCAGGC GCACGTTCAG TGTGAGGGTT TGTCTGAGCA ATTAGTATTT 
AACTCTGTTA CAAACTGCCT GGGCCCACGC AAGTTCCTGC ACAGTGGGAA ACTGTACAAA 
GCCAAGAGCA ACAAGGAACT ATACGGCTTC CTGTTCAACG ACTTCCTGCT GCTCACCCAG 
ATCATTAAGC CCCTCGGCTC CTCCGGCAAC GATAAGGTGT TCAGCCCCAA GTCCAATCTC 
CAGTACAAGA TGTACAAAAC TCCAATATTC CTCAACGAAG TGCTGGTGAA ACTGCCCACG 
GACCCATCCG GGGATGAGCC AATCTTTCAT ATTTCTCACA TTGACCGAGT CTACACATTG 
CGTGCAGAGA GCATCAATGA GAGGACGGCC TGGGTGCAGA AAATCAAAGC GGCATCGGAG 
TTGTACATAG AGACGGAGAA GAAGAAGAGA GAGAAGGCCT ACTTGGTTCG CTCCCAACGC 
GCAACAGGCA TCGGAAGACT GATGGTAAAT ATCGTTGAGG GAATTGAGTT GAAGCCCTGC 
AGAACCCACG GGAAGAGCAA TCCTTACTGT GAGATCACCA TGGGCTCCCA GTGCCACATT 
ACCAAGACCA TACAGGACAC CCTGAACCCC AAGTGGAACT CCAACTGCCA ATTCTTTATC 
AAGGACCTGG AGCAGGACGT TCTGTGCATC ACCGTGTTTG AGAGGGACCA GTTTTCCCCT 
GACGATTTCC TGGGCCGGAC AGAAATCCGA GTTGCTGACA TAAAGAAAGA CCAAGGTTCT 
AAAGGACCAG TAACCAAGTG TCTACTTCTG CACGAAGTTC CTACTGGAGA GATAGTGGTC 
CGATTGGATC TGCAGCTGTT TGACGAGCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	itsn1
Entrez Gene ID	733480
Full Name	intersectin 1
General protein information	Preferred Names intersectin 1 Names intersectin-1 intersectin 1 (SH3 domain protein)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Homo sapiens (human)	ITSN1	NP_003015.2
Canis lupus familiaris (dog)	ITSN1	XP_005638937.1
Drosophila melanogaster (fruit fly)	Dap160	NP_001246118.1
Xenopus tropicalis (tropical clawed frog)	itsn1	XP_002939390.1
Bos taurus (cattle)	ITSN1	XP_002707731.1
Mus musculus (house mouse)	Itsn1	NP_034717.2
Gallus gallus (chicken)	ITSN1	XP_416715.2
	ITSN1	XP_002803192.1
Rattus norvegicus (Norway rat)	Itsn1	NP_001129568.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009037	XP_319789.4
Pan troglodytes (chimpanzee)	ITSN1	XP_001166935.1
Canis lupus familiaris (dog)	LOC100856635	XP_003640127.1
Danio rerio (zebrafish)	itsn1	NP_997065.1
Caenorhabditis elegans (roundworm)	itsn-1	NP_001255927.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

itsn1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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