Hyal2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hyal2
Entrez Gene ID	15587
Full Name	hyaluronoglucosaminidase 2
Synonyms	AI256841,AU020858,AW555733
General protein information	Preferred Names hyaluronoglucosaminidase 2 Names hyaluronidase-2 hyal-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 F1\|9 58.12 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010489.2	NP_034619.2	hyaluronidase-2 precursor
XM_006511643.3	XP_006511706.1	hyaluronidase-2 isoform X1
XM_006511645.3	XP_006511708.1	hyaluronidase-2 isoform X1
XM_006511645.4	XP_006511708.1	hyaluronidase-2 isoform X1
XM_006511643.4	XP_006511706.1	hyaluronidase-2 isoform X1
XM_006511644.4	XP_006511707.1	hyaluronidase-2 isoform X1
XM_006511644.3	XP_006511707.1	hyaluronidase-2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HYAL2	XP_001168599.1
Danio rerio (zebrafish)	hyal2	XP_687640.2
Rattus norvegicus (Norway rat)	Hyal2	NP_742037.2
Danio rerio (zebrafish)	LOC100330444	XP_002662593.3
Danio rerio (zebrafish)	LOC567869	XP_005166205.1
Homo sapiens (human)	HYAL2	NP_149348.2
	HYAL2	XP_001101134.1
Bos taurus (cattle)	HYAL2	NP_776772.1
Gallus gallus (chicken)	HYAL2	XP_414258.2
Canis lupus familiaris (dog)	HYAL2	XP_005632567.1
Mus musculus (house mouse)	Hyal2	NP_034619.2
Xenopus tropicalis (tropical clawed frog)	hyal2	NP_001119986.1

Gene Ontology
Bibliography

Related articles in PubMed

Conditional knockdown of hyaluronidase 2 in articular cartilage stimulates osteoarthritic progression in a mice model.
Higuchi Y, Nishida Y, Kozawa E, Zhuo L, Arai E, Hamada S, Morita D, Ikuta K, Kimata K, Ushida T, Ishiguro N
Scientific reports7(1)7028(2017 Aug)

Hyaluronidase 2 Deficiency Causes Increased Mesenchymal Cells, Congenital Heart Defects, and Heart Failure.
Chowdhury B, Xiang B, Liu M, Hemming R, Dolinsky VW, Triggs-Raine B
Circulation. Cardiovascular genetics10(1)(2017 Jan)

Mutations in HYAL2, Encoding Hyaluronidase 2, Cause a Syndrome of Orofacial Clefting and Cor Triatriatum Sinister in Humans and Mice.
Muggenthaler MM, Chowdhury B, Hasan SN, Cross HE, Mark B, Harlalka GV, Patton MA, Ishida M, Behr ER, Sharma S, Zahka K, Faqeih E, Blakley B, Jackson M, Lees M, Dolinsky V, Cross L, Stanier P, Salter C, Baple EL, Alkuraya FS, Crosby AH, Triggs-Raine B, Chioza BA
PLoS genetics13(1)e1006470(2017 Jan)

Hyaluronan Depolymerization by Megakaryocyte Hyaluronidase-2 Is Required for Thrombopoiesis.
Petrey AC, Obery DR, Kessler SP, Flamion B, de la Motte CA
The American journal of pathology186(9)2390-403(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hyal2(-/-) mice have increased hyaluronan, which may promote endothelial-to-mesenchymal transition and proliferation of mesenchymal cells leading to congenital heart defects and heart failure.
Title: Hyaluronidase 2 Deficiency Causes Increased Mesenchymal Cells, Congenital Heart Defects, and Heart Failure.

Hyal2-/- mice present craniofacial abnormalities, including submucosal cleft palate and incompletely penetrant cor triatriatum sinister with hearing loss.
Title: Mutations in HYAL2, Encoding Hyaluronidase 2, Cause a Syndrome of Orofacial Clefting and Cor Triatriatum Sinister in Humans and Mice.

our data uncover a previously unsuspected mechanism of how hyaluronan and Hyal-2 control platelet generation.
Title: Hyaluronan Depolymerization by Megakaryocyte Hyaluronidase-2 Is Required for Thrombopoiesis.

It was concluded that: HYAL1 and HYAL2 are both needed for tissue HA catabolism; 2) HYAL2 is required for high MW HA clearance in lymph nodes and plasma and for HA endocytosis by liver NPCs; and 3) the main role of HYAL1 is HA degradation within liver NPCs.
Title: Respective roles of hyaluronidases 1 and 2 in endogenous hyaluronan turnover.

Removal of Hyal2 activity resulted in increased levels of hyaluronan creating a risk for cor triatriatum sinister.
Title: Hyaluronidase 2 deficiency is a molecular cause of cor triatriatum sinister in mice.

The following Hyal2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hyal2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu06298	NM_010489.2 Latest version!	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu06298	XM_006511645.4 Latest version!	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu06298	XM_006511643.4 Latest version!	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu06298	XM_006511644.4 Latest version!	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu06298	XM_006511645.3	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu06298	XM_006511643.3	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu06298	XM_006511644.3	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hyal2 ( NM_010489.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034619.2 Mus musculus hyaluronoglucosaminidase 2 (Hyal2), mRNA.

Price & Availability↑

CloneID	OMu06298
Clone ID Related Accession (Same CDS sequence)	NM_010489.2 , XM_006511643.3 , XM_006511645.3 , XM_006511645.4 , XM_006511643.4 , XM_006511644.4 , XM_006511644.3
Accession Version	NM_010489.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	hyaluronidase-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF422177.1. On Mar 10, 2004 this sequence version replaced NM_010489.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF422177.1, AJ000059.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034619.2
CDS	118..1539
Translation	MRAGLGPIITLALVLEVAWAGELKPTAPPIFTGRPFVVAWNVPTQECAPRHKVPLDLRAFDVKATPNEGFFNQNITTFYYDRLGLYPRFDAAGTSVHGGVPQNGSLCAHLPMLKESVERYIQTQEPGGLAVIDWEEWRPVWVRNWQEKDVYRQSSRQLVASRHPDWPSDRVMKQAQYEFEFAARQFMLNTLRYVKAVRPQHLWGFYLFPDCYNHDYVQNWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSVHSRNFVSFRVREALRVAHTHHANHALPVYVFTRPTYTRGLTGLSQVDLISTIGESAALGSAGVIFWGDSEDASSMETCQYLKNYLTQLLVPYIVNVSWATQYCSWTQCHGHGRCVRRNPSANTFLHLNASSFRLVPGHTPSEPQLRPEGQLSEADLNYLQKHFRCQCYLGWGGEQCQRNYKGAAGNASRAWAGSHLTSLLGLVAVALTWTL

Target ORF information:

RefSeq Version	NM_010489.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010489.2

ORF Insert Sequence:

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hyal2 ( XM_006511643.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511706.1 Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu06298
Clone ID Related Accession (Same CDS sequence)	NM_010489.2 , XM_006511643.3 , XM_006511645.3 , XM_006511645.4 , XM_006511643.4 , XM_006511644.4 , XM_006511644.3
Accession Version	XM_006511643.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	hyaluronidase-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511643.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511706.1
CDS	380..1801
Translation	MRAGLGPIITLALVLEVAWAGELKPTAPPIFTGRPFVVAWNVPTQECAPRHKVPLDLRAFDVKATPNEGFFNQNITTFYYDRLGLYPRFDAAGTSVHGGVPQNGSLCAHLPMLKESVERYIQTQEPGGLAVIDWEEWRPVWVRNWQEKDVYRQSSRQLVASRHPDWPSDRVMKQAQYEFEFAARQFMLNTLRYVKAVRPQHLWGFYLFPDCYNHDYVQNWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSVHSRNFVSFRVREALRVAHTHHANHALPVYVFTRPTYTRGLTGLSQVDLISTIGESAALGSAGVIFWGDSEDASSMETCQYLKNYLTQLLVPYIVNVSWATQYCSWTQCHGHGRCVRRNPSANTFLHLNASSFRLVPGHTPSEPQLRPEGQLSEADLNYLQKHFRCQCYLGWGGEQCQRNYKGAAGNASRAWAGSHLTSLLGLVAVALTWTL

Target ORF information:

RefSeq Version	XM_006511643.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511643.3

ORF Insert Sequence:

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hyal2 ( XM_006511645.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511708.1 Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu06298
Clone ID Related Accession (Same CDS sequence)	NM_010489.2 , XM_006511643.3 , XM_006511645.3 , XM_006511645.4 , XM_006511643.4 , XM_006511644.4 , XM_006511644.3
Accession Version	XM_006511645.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	hyaluronidase-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511645.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511708.1
CDS	226..1647
Translation	MRAGLGPIITLALVLEVAWAGELKPTAPPIFTGRPFVVAWNVPTQECAPRHKVPLDLRAFDVKATPNEGFFNQNITTFYYDRLGLYPRFDAAGTSVHGGVPQNGSLCAHLPMLKESVERYIQTQEPGGLAVIDWEEWRPVWVRNWQEKDVYRQSSRQLVASRHPDWPSDRVMKQAQYEFEFAARQFMLNTLRYVKAVRPQHLWGFYLFPDCYNHDYVQNWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSVHSRNFVSFRVREALRVAHTHHANHALPVYVFTRPTYTRGLTGLSQVDLISTIGESAALGSAGVIFWGDSEDASSMETCQYLKNYLTQLLVPYIVNVSWATQYCSWTQCHGHGRCVRRNPSANTFLHLNASSFRLVPGHTPSEPQLRPEGQLSEADLNYLQKHFRCQCYLGWGGEQCQRNYKGAAGNASRAWAGSHLTSLLGLVAVALTWTL

Target ORF information:

RefSeq Version	XM_006511645.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511645.3

ORF Insert Sequence:

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hyal2 ( XM_006511644.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511707.1 Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu06298
Clone ID Related Accession (Same CDS sequence)	NM_010489.2 , XM_006511643.3 , XM_006511645.3 , XM_006511645.4 , XM_006511643.4 , XM_006511644.4 , XM_006511644.3
Accession Version	XM_006511644.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	hyaluronidase-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511644.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511707.1
CDS	138..1559
Translation	MRAGLGPIITLALVLEVAWAGELKPTAPPIFTGRPFVVAWNVPTQECAPRHKVPLDLRAFDVKATPNEGFFNQNITTFYYDRLGLYPRFDAAGTSVHGGVPQNGSLCAHLPMLKESVERYIQTQEPGGLAVIDWEEWRPVWVRNWQEKDVYRQSSRQLVASRHPDWPSDRVMKQAQYEFEFAARQFMLNTLRYVKAVRPQHLWGFYLFPDCYNHDYVQNWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSVHSRNFVSFRVREALRVAHTHHANHALPVYVFTRPTYTRGLTGLSQVDLISTIGESAALGSAGVIFWGDSEDASSMETCQYLKNYLTQLLVPYIVNVSWATQYCSWTQCHGHGRCVRRNPSANTFLHLNASSFRLVPGHTPSEPQLRPEGQLSEADLNYLQKHFRCQCYLGWGGEQCQRNYKGAAGNASRAWAGSHLTSLLGLVAVALTWTL

Target ORF information:

RefSeq Version	XM_006511644.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511644.3

ORF Insert Sequence:

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hyal2 ( XM_006511645.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511708.1 Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu06298
Clone ID Related Accession (Same CDS sequence)	NM_010489.2 , XM_006511643.3 , XM_006511645.3 , XM_006511645.4 , XM_006511643.4 , XM_006511644.4 , XM_006511644.3
Accession Version	XM_006511645.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	hyaluronidase-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511645.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511708.1
CDS	147..1568
Translation	MRAGLGPIITLALVLEVAWAGELKPTAPPIFTGRPFVVAWNVPTQECAPRHKVPLDLRAFDVKATPNEGFFNQNITTFYYDRLGLYPRFDAAGTSVHGGVPQNGSLCAHLPMLKESVERYIQTQEPGGLAVIDWEEWRPVWVRNWQEKDVYRQSSRQLVASRHPDWPSDRVMKQAQYEFEFAARQFMLNTLRYVKAVRPQHLWGFYLFPDCYNHDYVQNWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSVHSRNFVSFRVREALRVAHTHHANHALPVYVFTRPTYTRGLTGLSQVDLISTIGESAALGSAGVIFWGDSEDASSMETCQYLKNYLTQLLVPYIVNVSWATQYCSWTQCHGHGRCVRRNPSANTFLHLNASSFRLVPGHTPSEPQLRPEGQLSEADLNYLQKHFRCQCYLGWGGEQCQRNYKGAAGNASRAWAGSHLTSLLGLVAVALTWTL

Target ORF information:

RefSeq Version	XM_006511645.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511645.4

ORF Insert Sequence:

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hyal2 ( XM_006511643.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511706.1 Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu06298
Clone ID Related Accession (Same CDS sequence)	NM_010489.2 , XM_006511643.3 , XM_006511645.3 , XM_006511645.4 , XM_006511643.4 , XM_006511644.4 , XM_006511644.3
Accession Version	XM_006511643.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	hyaluronidase-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511643.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511706.1
CDS	378..1799
Translation	MRAGLGPIITLALVLEVAWAGELKPTAPPIFTGRPFVVAWNVPTQECAPRHKVPLDLRAFDVKATPNEGFFNQNITTFYYDRLGLYPRFDAAGTSVHGGVPQNGSLCAHLPMLKESVERYIQTQEPGGLAVIDWEEWRPVWVRNWQEKDVYRQSSRQLVASRHPDWPSDRVMKQAQYEFEFAARQFMLNTLRYVKAVRPQHLWGFYLFPDCYNHDYVQNWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSVHSRNFVSFRVREALRVAHTHHANHALPVYVFTRPTYTRGLTGLSQVDLISTIGESAALGSAGVIFWGDSEDASSMETCQYLKNYLTQLLVPYIVNVSWATQYCSWTQCHGHGRCVRRNPSANTFLHLNASSFRLVPGHTPSEPQLRPEGQLSEADLNYLQKHFRCQCYLGWGGEQCQRNYKGAAGNASRAWAGSHLTSLLGLVAVALTWTL

Target ORF information:

RefSeq Version	XM_006511643.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511643.4

ORF Insert Sequence:

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hyal2 ( XM_006511644.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511707.1 Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X2, mRNA.

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CloneID	OMu06298
Clone ID Related Accession (Same CDS sequence)	NM_010489.2 , XM_006511643.3 , XM_006511645.3 , XM_006511645.4 , XM_006511643.4 , XM_006511644.4 , XM_006511644.3
Accession Version	XM_006511644.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	hyaluronidase-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511644.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511707.1
CDS	151..1572
Translation	MRAGLGPIITLALVLEVAWAGELKPTAPPIFTGRPFVVAWNVPTQECAPRHKVPLDLRAFDVKATPNEGFFNQNITTFYYDRLGLYPRFDAAGTSVHGGVPQNGSLCAHLPMLKESVERYIQTQEPGGLAVIDWEEWRPVWVRNWQEKDVYRQSSRQLVASRHPDWPSDRVMKQAQYEFEFAARQFMLNTLRYVKAVRPQHLWGFYLFPDCYNHDYVQNWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSVHSRNFVSFRVREALRVAHTHHANHALPVYVFTRPTYTRGLTGLSQVDLISTIGESAALGSAGVIFWGDSEDASSMETCQYLKNYLTQLLVPYIVNVSWATQYCSWTQCHGHGRCVRRNPSANTFLHLNASSFRLVPGHTPSEPQLRPEGQLSEADLNYLQKHFRCQCYLGWGGEQCQRNYKGAAGNASRAWAGSHLTSLLGLVAVALTWTL

Target ORF information:

RefSeq Version	XM_006511644.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus hyaluronoglucosaminidase 2 (Hyal2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511644.4

ORF Insert Sequence:

ATGCGGGCAG GACTAGGTCC CATCATCACA CTGGCCCTAG TGCTGGAGGT AGCATGGGCC 
GGGGAGCTTA AGCCCACAGC GCCGCCCATC TTCACTGGCC GACCCTTTGT GGTAGCATGG 
AACGTGCCCA CACAAGAATG TGCCCCACGC CACAAAGTGC CACTGGACCT TAGGGCCTTC 
GATGTGAAGG CTACACCGAA TGAGGGTTTT TTCAACCAGA ATATCACCAC CTTCTACTAC 
GACCGTCTAG GCCTGTATCC ACGTTTTGAT GCAGCCGGGA CATCTGTGCA TGGCGGTGTG 
CCTCAGAACG GCAGCCTCTG TGCACACCTG CCCATGCTGA AGGAATCTGT GGAACGCTAC 
ATCCAGACCC AGGAGCCTGG GGGGCTGGCA GTCATTGACT GGGAGGAATG GCGGCCTGTA 
TGGGTTCGAA ACTGGCAGGA GAAAGATGTT TACCGACAGT CTTCACGCCA GCTGGTGGCC 
AGTCGGCACC CTGACTGGCC ATCAGACAGA GTAATGAAGC AGGCCCAGTA CGAGTTTGAG 
TTCGCCGCTC GGCAGTTCAT GTTGAACACT CTCCGTTACG TCAAGGCAGT CAGACCCCAG 
CACCTGTGGG GCTTCTACCT CTTTCCTGAC TGCTACAATC ACGATTATGT ACAGAACTGG 
GAGAGCTACA CGGGCCGCTG TCCCGATGTG GAGGTGGCAC GGAACGACCA GCTGGCCTGG 
CTCTGGGCTG AGAGCACGGC TCTCTTTCCC TCTGTGTACC TGGACGAGAC ACTGGCGTCC 
TCCGTACACA GCCGCAACTT TGTCAGTTTC CGTGTTCGGG AGGCCCTTCG AGTGGCTCAC 
ACCCACCATG CCAACCACGC CCTCCCCGTG TACGTCTTCA CGCGTCCCAC ATACACCCGA 
GGACTCACGG GACTGAGCCA GGTGGACCTT ATCTCTACCA TCGGTGAGAG TGCCGCCCTG 
GGCTCAGCTG GCGTCATCTT CTGGGGCGAC TCGGAAGACG CTTCAAGTAT GGAGACCTGC 
CAATACCTCA AGAATTACCT AACTCAGCTG CTGGTTCCCT ACATAGTCAA CGTGTCCTGG 
GCCACCCAGT ATTGCAGTTG GACCCAGTGC CATGGCCATG GGCGATGTGT GCGCCGCAAC 
CCCAGCGCCA ATACCTTCCT GCACCTCAAT GCCAGCAGCT TCCGCCTAGT GCCTGGCCAT 
ACCCCCAGTG AACCCCAGCT TCGACCCGAG GGGCAGCTCA GCGAAGCCGA CCTCAACTAC 
CTGCAGAAGC ACTTTCGCTG CCAGTGCTAT CTGGGCTGGG GTGGCGAGCA GTGCCAACGG 
AACTATAAGG GGGCAGCTGG AAATGCCAGC AGAGCCTGGG CTGGATCCCA CCTCACCAGC 
CTGCTGGGTT TGGTAGCTGT GGCTCTCACC TGGACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Conditional knockdown of hyaluronidase 2 in articular cartilage stimulates osteoarthritic progression in a mice model.
Scientific reports7(1)7028(2017 Aug)
Higuchi Y,Nishida Y,Kozawa E,Zhuo L,Arai E,Hamada S,Morita D,Ikuta K,Kimata K,Ushida T,Ishiguro N

Hyaluronidase 2 Deficiency Causes Increased Mesenchymal Cells, Congenital Heart Defects, and Heart Failure.
Circulation. Cardiovascular genetics10(1)(2017 Jan)
Chowdhury B,Xiang B,Liu M,Hemming R,Dolinsky VW,Triggs-Raine B

Mutations in HYAL2, Encoding Hyaluronidase 2, Cause a Syndrome of Orofacial Clefting and Cor Triatriatum Sinister in Humans and Mice.
PLoS genetics13(1)e1006470(2017 Jan)
Muggenthaler MM,Chowdhury B,Hasan SN,Cross HE,Mark B,Harlalka GV,Patton MA,Ishida M,Behr ER,Sharma S,Zahka K,Faqeih E,Blakley B,Jackson M,Lees M,Dolinsky V,Cross L,Stanier P,Salter C,Baple EL,Alkuraya FS,Crosby AH,Triggs-Raine B,Chioza BA

Hyaluronan Depolymerization by Megakaryocyte Hyaluronidase-2 Is Required for Thrombopoiesis.
The American journal of pathology186(9)2390-403(2016 Sep)
Petrey AC,Obery DR,Kessler SP,Flamion B,de la Motte CA

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Hyal2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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