Dnmt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnmt1
Entrez Gene ID	13433
Full Name	DNA methyltransferase (cytosine-5) 1
Synonyms	Cxxc9,Dnmt,Dnmt1o,MCMT,MTase,Met-1,Met1,MommeD2,m.MmuI
General protein information	Preferred Names DNA methyltransferase (cytosine-5) 1 Names DNA (cytosine-5)-methyltransferase 1 DNA MTase MmuI DNA methyltransferase MmuI
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 A3\|9 7.66 cM
Summary	This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010066.4	NP_034196.5	DNA (cytosine-5)-methyltransferase 1 isoform 2
XM_011242393.2	XP_011240695.1	DNA (cytosine-5)-methyltransferase 1 isoform X2
NM_001199433.1	NP_001186362.1	DNA (cytosine-5)-methyltransferase 1 isoform 4
NM_001199431.1	NP_001186360.2	DNA (cytosine-5)-methyltransferase 1 isoform 1
NM_001314011.1	NP_001300940.1	DNA (cytosine-5)-methyltransferase 1 isoform 5
NM_001199432.1	NP_001186361.1	DNA (cytosine-5)-methyltransferase 1 isoform 3
XM_006509988.2	XP_006510051.1	DNA (cytosine-5)-methyltransferase 1 isoform X1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	MEE57	NP_193097.1
Homo sapiens (human)	DNMT1	NP_001124295.1
Bos taurus (cattle)	DNMT1	NP_872592.2
Danio rerio (zebrafish)	dnmt1	NP_571264.1
Oryza sativa (rice)	Os03g0798300	NP_001051569.1
Oryza sativa (rice)	Os07g0182900	NP_001059052.1
Canis lupus familiaris (dog)	DNMT1	XP_533919.4
Arabidopsis thaliana (thale cress)	AT4G08990	NP_192638.1
Xenopus tropicalis (tropical clawed frog)	dnmt1	XP_002934384.1
Pan troglodytes (chimpanzee)	DNMT1	XP_512361.3
	DNMT1	XP_001104704.2
Mus musculus (house mouse)	Dnmt1	NP_001186360.2
Rattus norvegicus (Norway rat)	Dnmt1	NP_445806.3
Arabidopsis thaliana (thale cress)	DMT2	NP_193150.1
Arabidopsis thaliana (thale cress)	MET1	NP_199727.1
Gallus gallus (chicken)	DNMT1	NP_996835.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of DNMT1-DNA complex reveals a role for autoinhibition in maintenance DNA methylation.
Song J, Rechkoblit O, Bestor TH, Patel DJ
Science (New York, N.Y.)331(6020)1036-40(2011 Feb)

Biological functions of DNA methyltransferase 1 require its methyltransferase activity.
Damelin M, Bestor TH
Molecular and cellular biology27(11)3891-9(2007 Jun)

Stage-by-stage change in DNA methylation status of Dnmt1 locus during mouse early development.
Ko YG, Nishino K, Hattori N, Arai Y, Tanaka S, Shiota K
The Journal of biological chemistry280(10)9627-34(2005 Mar)

The activity of the murine DNA methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA.
Fatemi M, Hermann A, Pradhan S, Jeltsch A
Journal of molecular biology309(5)1189-99(2001 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data from studies using mouse embryonic fibroblasts suggest that cell proliferation rate positively correlates with expression of Dnmt1 in G1 phase; global DNA methylation is significantly higher in G1 phase than in G2/M phase; larger methylation differences are observed on promoters of pluripotency-related genes; thus, high cell proliferation rates promote generation of induced pluripotent stem cells.
Title: Passive DNA demethylation preferentially up-regulates pluripotency-related genes and facilitates the generation of induced pluripotent stem cells.

The lethal Ogden syndrome-associated mutation of Naa10p disrupts its binding to the imprinting control region of H19 and Dnmt1 recruitment.
Title: The Role of N-?-acetyltransferase 10 Protein in DNA Methylation and Genomic Imprinting.

The lack of Sirt7 is associated with reduced recruitment of DNMT1 and Sirt1 at rRNA genes leading to hyperacetylation of histones, reduced DNA methylation, fragmentation of the nucleolar structure and loss of rDNA repeats leading to anincreased spontaneous immortalization of primary mouse embryonic fibroblasts.
Title: Sirt7 stabilizes rDNA heterochromatin through recruitment of DNMT1 and Sirt1.

During neurogenesis, cortical neurons became protected from S-phase Chk1 pathway activation by the DNA methyltransferase Dnmt1, and underwent cell death after S-phase progression.
Title: Dnmt1-dependent Chk1 pathway suppression is protective against neuron division.

reciprocal regulation between miR-148a/152 and DNMT1 in foam cells
Title: Reciprocal Regulation Between miR-148a/152 and DNA Methyltransferase 1 Is Associated with Hyperhomocysteinemia-Accelerated Atherosclerosis.

The following Dnmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu83580	NM_001314011.1 Latest version!	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$811.30 $1159.00
OMu02259	NM_001199433.1 Latest version!	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$811.30 $1159.00
OMu67558	XM_011242393.2 Latest version!	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$811.30 $1159.00
OMu02256	NM_001199432.1 Latest version!	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu15704	NM_001199431.1 Latest version!	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu15992	NM_010066.4 Latest version!	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu41988	XM_006509988.2 Latest version!	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnmt1 ( NM_001314011.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300940.1 Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 5, mRNA.

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CloneID	OMu83580
Clone ID Related Accession (Same CDS sequence)	NM_001314011.1
Accession Version	NM_001314011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	DNA (cytosine-5)-methyltransferase 1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF162282.1, BC053047.1 and AK147143.1. On Sep 24, 2015 this sequence version replaced XM_006509987.1. Transcript Variant: This variant (5) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. It encodes isoform 5, which is longer than isoform 1. This variant has two upstream in-frame AUGs but N-terminal sequencing and western analysis from PMID: 9830015 (Table I and Figure 2) support the use of the third in-frame AUG. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053047.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9830015) ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCAGCGC GAACAGCTCC AGCCCGAGTG CCTGCGCTTG CCTCCCCGGC AGGCTCGCTC 
CCGGACCATG TCCGCAGGCG GCTCAAAGAC TTGGAAAGAG ATGGCTTAAC AGAAAAGGAG 
TGTGTGAGGG AGAAATTAAA CTTACTGCAT GAATTCCTGC AAACAGAAAT AAAAAGCCAG 
TTGTGTGACT TGGAAACCAA ATTACATAAA GAGGAATTAT CTGAGGAAGG CTACCTGGCT 
AAAGTCAAGT CCCTCTTAAA TAAGGATTTG TCCTTGGAGA ACGGAACACA CACTCTCACT 
CAAAAAGCCA ACGGTTGTCC CGCCAACGGG AGCCGGCCAA CCTGGAGAGC AGAAATGGCA 
GACTCAAATA GATCCCCAAG ATCCAGGCCC AAGCCTCGGG GACCCAGGAG AAGCAAGTCG 
GACAGTGACA CCCTTTGTAA GGACACGAGA CATACTGCAG TTGAAACTTC ACCTAGTTCC 
GTGGCTACGA GGAGAACCAC CAGGCAGACC ACCATCACGG CTCACTTCAC GAAGGGCCCC 
ACTAAACGGA AACCCAAGGA AGAGTCGGAA GAGGGGAACT CGGCTGAGTC GGCTGCAGAG 
GAGAGAGACC AGGATAAGAA ACGCAGAGTT GTAGACACAG AGAGTGGTGC TGCAGCTGCT 
GTGGAGAAAC TGGAAGAGGT AACAGCGGGA ACCCAGCTGG GTCCGGAAGA GCCATGTGAA 
CAGGAAGATG ACAACAGGAG TCTTCGACGT CACACCAGAG AGCTATCATT GAGGCGGAAA 
TCAAAGGAGG ATCCAGACAG AGAAGCAAGA CCGGAAACTC ACTTGGACGA GGACGAGGAC 
GGAAAAAAGG ATAAAAGAAG TTCCAGACCC AGGAGCCAGC CCAGAGATCC AGCTGCCAAA 
CGGAGACCCA AGGAAGCAGA GCCAGAGCAG GTAGCTCCAG AGACTCCCGA GGACAGAGAC 
GAGGATGAGA GGGAGGAGAA GAGACGAAAA ACGACACGTA AAAAACTGGA GTCACACACC 
GTTCCCGTTC AGAGCAGATC GGAGAGAAAA GCCGCTCAAA GCAAAAGTGT GATCCCGAAG 
ATCAACTCAC CAAAGTGCCC CGAGTGTGGC CAGCACCTAG ACGACCCTAA CCTGAAGTAC 
CAGCAGCACC CTGAGGATGC TGTGGATGAA CCCCAGATGT TGACCAGTGA GAAACTGTCC 
ATCTACGACT CCACCTCGAC CTGGTTTGAT ACTTATGAAG ATTCTCCCAT GCATAGGTTC 
ACTTCCTTCA GTGTGTACTG CAGTCGCGGG CACCTGTGTC CTGTCGACAC CGGTCTCATT 
GAGAAGAATG TAGAGCTCTA CTTTTCTGGG TGTGCCAAAG CAATTCATGA CGAGAATCCA 
TCTATGGAAG GTGGTATTAA TGGCAAAAAC CTCGGGCCAA TCAATCAGTG GTGGCTCAGT 
GGCTTTGATG GTGGCGAGAA GGTGCTCATT GGCTTCTCCA CTGCATTTGC TGAATACATT 
TTGATGGAGC CCAGCAAAGA GTATGAGCCA ATATTTGGGC TGATGCAGGA GAAAATTTAC 
ATCAGCAAGA TTGTTGTTGA GTTCCTGCAA AACAATCCTG ATGCTGTATA TGAAGACCTG 
ATCAATAAGA TTGAGACCAC TGTTCCTCCT TCTACCATTA ATGTGAACCG GTTCACAGAG 
GACTCCCTCT TACGCCACGC CCAGTTTGTA GTGAGCCAGG TAGAGAGTTA CGACGAAGCC 
AAGGACGATG ATGAGACCCC CATCTTCTTG TCTCCCTGTA TGAGAGCCCT GATCCATTTG 
GCTGGTGTCT CCCTGGGACA GAGGCGAGCA ACAAGGCGCG TCATGGGTGC TACCAAGGAG 
AAGGACAAAG CACCCACGAA AGCCACCACC ACCAAGCTGG TCTATCAGAT CTTTGACACT 
TTCTTCTCAG AGCAGATTGA GAAGTATGAT AAGGAGGACA AGGAGAATGC CATGAAGCGC 
CGCCGCTGTG GTGTCTGTGA GGTCTGTCAG CAGCCTGAGT GTGGGAAGTG CAAGGCGTGC 
AAAGATATGG TGAAGTTTGG TGGCACTGGA CGGAGTAAGC AGGCTTGCCT CAAGAGGAGG 
TGTCCTAACT TGGCGGTGAA GGAGGCAGAC GACGATGAAG AGGCTGATGA TGATGTGTCA 
GAGATGCCAT CACCCAAAAA GCTGCATCAG GGGAAGAAGA AGAAGCAGAA CAAGGACCGC 
ATCTCCTGGC TTGGGCAGCC TATGAAGATT GAAGAGAATA GAACTTACTA TCAGAAGGTG 
AGCATCGATG AGGAGATGCT AGAGGTGGGC GACTGCGTCT CGGTCATTCC AGATGATTCC 
TCCAAACCAC TCTATCTAGC CAGGGTCACA GCTCTGTGGG AAGACAAAAA TGGTCAGATG 
ATGTTCCATG CGCACTGGTT CTGCGCTGGG ACAGACACAG TCCTGGGAGC CACCTCCGAC 
CCCCTGGAAC TGTTCCTGGT GGGCGAGTGC GAAAACATGC AGCTTTCCTA CATCCACAGC 
AAGGTCAAGG TCATCTACAA AGCCCCTTCT GAAAACTGGG CCATGGAGGG AGGCACAGAC 
CCTGAGACCA CACTGCCTGG GGCTGAGGAT GGCAAGACTT ACTTCTTCCA GCTCTGGTAC 
AACCAGGAGT ACGCAAGGTT TGAATCCCCA CCCAAGACCC AGCCGACCGA GGACAACAAG 
CACAAGTTCT GCCTATCTTG TATCCGGCTG GCTGAGCTGA GACAAAAAGA AATGCCCAAG 
GTCCTGGAAC AAATTGAGGA GGTGGATGGC CGGGTCTACT GCAGTTCCAT CACCAAGAAT 
GGTGTTGTCT ACCGACTGGG TGACAGTGTG TACCTTCCTC CCGAGGCCTT TACTTTCAAC 
ATCAAAGTGG CTAGCCCCGT GAAACGCCCA AAGAAGGATC CTGTGAACGA GACCCTGTAC 
CCTGAGCACT ACCGCAAGTA TTCTGACTAC ATCAAGGGGA GCAACCTGGA TGCTCCAGAG 
CCCTATCGCA TCGGTCGGAT AAAAGAGATC CACTGTGGCA AGAAGAAAGG CAAGGTCAAC 
GAGGCAGACA TCAAGCTGAG GCTCTACAAG TTCTACAGGC CTGAGAATAC CCACAGGTCC 
TACAACGGAT CCTATCACAC TGACATCAAC ATGCTTTACT GGAGCGACGA GGAAGCTGTG 
GTGAACTTCA GCGACGTGCA GGGCCGCTGT ACCGTGGAGT ACGGGGAAGA CCTACTTGAG 
AGCATCCAGG ATTATTCACA AGGGGGCCCT GACCGCTTCT ACTTCCTCGA GGCCTACAAT 
TCAAAGACCA AGAACTTTGA AGACCCACCA AACCATGCCC GCAGCCCTGG GAACAAAGGG 
AAAGGGAAGG GGAAAGGGAA GGGGAAGGGG AAGCATCAGG TGTCAGAGCC CAAAGAGCCT 
GAGGCAGCCA TCAAACTGCC CAAGCTCCGG ACCCTGGATG TGTTTTCCGG CTGTGGAGGG 
TTATCGGAAG GATTCCACCA AGCAGGCATC TCGGAAACGC TGTGGGCCAT CGAGATGTGG 
GACCCGGCAG CCCAGGCATT TCGGCTGAAC AACCCCGGCA CCACAGTGTT CACAGAGGAC 
TGCAACGTGC TTCTTAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCTCT GGGCCAAAGG 
CTGCCACAGA AGGGCGATGT GGAGATGCTG TGTGGTGGGC CACCCTGCCA GGGCTTCAGT 
GGCATGAACC GCTTCAACTC CCGCACTTAC TCCAAGTTCA AAAACTCCCT AGTGGTCTCC 
TTCCTCAGCT ACTGTGACTA CTACCGGCCT CGGTTCTTCC TTCTGGAGAA CGTCAGGAAC 
TTCGTGTCCT ACAGACGCTC CATGGTGCTG AAGCTCACAC TGCGCTGCCT GGTCCGCATG 
GGCTACCAGT GCACCTTTGG TGTGCTCCAG GCTGGACAGT ATGGCGTGGC CCAGACACGA 
AGGAGGGCCA TCATCTTGGC TGCAGCCCCA GGAGAAAAGC TGCCTCTGTT CCCAGAGCCT 
CTGCATGTGT TTGCGCCCCG TGCCTGCCAG CTGAGCGTTG TGGTGGATGA CAAGAAGTTT 
GTTAGCAACA TAACGAGGCT GAGCTCGGGG CCCTTCCGAA CCATCACCGT GCGAGACACC 
ATGTCTGACC TCCCCGAGAT CCAGAATGGA GCCTCGAATT CTGAGATCCC CTACAATGGA 
GAGCCACTGT CCTGGTTCCA GAGGCAGCTG CGAGGATCAC ACTACCAGCC CATCCTCAGG 
GACCATATCT GCAAGGACAT GAGCCCACTG GTGGCTGCCC GCATGCGGCA CATCCCACTG 
TTCCCAGGAT CAGATTGGCG TGACCTGCCC AACATACAGG TGCGGCTGGG AGATGGCGTC 
ATAGCCCATA AGCTACAGTA CACCTTTCAT GATGTGAAAA ATGGCTACAG CAGCACCGGT 
GCCCTGCGTG GAGTCTGTTC CTGTGCAGAA GGCAAGGCCT GCGACCCTGA GTCCAGGCAA 
TTCAGCACCC TCATCCCCTG GTGCCTGCCG CACACTGGGA ACCGGCACAA CCACTGGGCT 
GGCCTCTACG GGCGTCTGGA GTGGGATGGC TTCTTCAGCA CCACTGTCAC CAACCCTGAG 
CCCATGGGCA AGCAGGGTCG GGTGCTCCAC CCGGAGCAGC ACCGGGTCGT GAGTGTTCGG 
GAATGTGCCC GCTCCCAGGG CTTTCCAGAT AGCTACCGGT TCTTCGGCAA CATCCTGGAC 
AGACACCGGC AGGTGGGTAA TGCTGTGCCA CCACCCCTGG CCAAAGCCAT TGGCCTGGAG 
ATTAAGCTCT GCCTGCTGTC CAGTGCTCGG GAGAGCGCAT CAGCTGCAGT TAAAGCAAAA 
GAGGAGGCTG CTACCAAGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300940.1
CDS	193..5076
Translation	MPARTAPARVPALASPAGSLPDHVRRRLKDLERDGLTEKECVREKLNLLHEFLQTEIKSQLCDLETKLHKEELSEEGYLAKVKSLLNKDLSLENGTHTLTQKANGCPANGSRPTWRAEMADSNRSPRSRPKPRGPRRSKSDSDTLCKDTRHTAVETSPSSVATRRTTRQTTITAHFTKGPTKRKPKEESEEGNSAESAAEERDQDKKRRVVDTESGAAAAVEKLEEVTAGTQLGPEEPCEQEDDNRSLRRHTRELSLRRKSKEDPDREARPETHLDEDEDGKKDKRSSRPRSQPRDPAAKRRPKEAEPEQVAPETPEDRDEDEREEKRRKTTRKKLESHTVPVQSRSERKAAQSKSVIPKINSPKCPECGQHLDDPNLKYQQHPEDAVDEPQMLTSEKLSIYDSTSTWFDTYEDSPMHRFTSFSVYCSRGHLCPVDTGLIEKNVELYFSGCAKAIHDENPSMEGGINGKNLGPINQWWLSGFDGGEKVLIGFSTAFAEYILMEPSKEYEPIFGLMQEKIYISKIVVEFLQNNPDAVYEDLINKIETTVPPSTINVNRFTEDSLLRHAQFVVSQVESYDEAKDDDETPIFLSPCMRALIHLAGVSLGQRRATRRVMGATKEKDKAPTKATTTKLVYQIFDTFFSEQIEKYDKEDKENAMKRRRCGVCEVCQQPECGKCKACKDMVKFGGTGRSKQACLKRRCPNLAVKEADDDEEADDDVSEMPSPKKLHQGKKKKQNKDRISWLGQPMKIEENRTYYQKVSIDEEMLEVGDCVSVIPDDSSKPLYLARVTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYIHSKVKVIYKAPSENWAMEGGTDPETTLPGAEDGKTYFFQLWYNQEYARFESPPKTQPTEDNKHKFCLSCIRLAELRQKEMPKVLEQIEEVDGRVYCSSITKNGVVYRLGDSVYLPPEAFTFNIKVASPVKRPKKDPVNETLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPENTHRSYNGSYHTDINMLYWSDEEAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSKTKNFEDPPNHARSPGNKGKGKGKGKGKGKHQVSEPKEPEAAIKLPKLRTLDVFSGCGGLSEGFHQAGISETLWAIEMWDPAAQAFRLNNPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEIQNGASNSEIPYNGEPLSWFQRQLRGSHYQPILRDHICKDMSPLVAARMRHIPLFPGSDWRDLPNIQVRLGDGVIAHKLQYTFHDVKNGYSSTGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDSYRFFGNILDRHRQVGNAVPPPLAKAIGLEIKLCLLSSARESASAAVKAKEEAATKD

Target ORF information:

RefSeq Version	NM_001314011.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001314011.1

ORF Insert Sequence:

ATGCCAGCGC GAACAGCTCC AGCCCGAGTG CCTGCGCTTG CCTCCCCGGC AGGCTCGCTC 
CCGGACCATG TCCGCAGGCG GCTCAAAGAC TTGGAAAGAG ATGGCTTAAC AGAAAAGGAG 
TGTGTGAGGG AGAAATTAAA CTTACTGCAT GAATTCCTGC AAACAGAAAT AAAAAGCCAG 
TTGTGTGACT TGGAAACCAA ATTACATAAA GAGGAATTAT CTGAGGAAGG CTACCTGGCT 
AAAGTCAAGT CCCTCTTAAA TAAGGATTTG TCCTTGGAGA ACGGAACACA CACTCTCACT 
CAAAAAGCCA ACGGTTGTCC CGCCAACGGG AGCCGGCCAA CCTGGAGAGC AGAAATGGCA 
GACTCAAATA GATCCCCAAG ATCCAGGCCC AAGCCTCGGG GACCCAGGAG AAGCAAGTCG 
GACAGTGACA CCCTTTGTAA GGACACGAGA CATACTGCAG TTGAAACTTC ACCTAGTTCC 
GTGGCTACGA GGAGAACCAC CAGGCAGACC ACCATCACGG CTCACTTCAC GAAGGGCCCC 
ACTAAACGGA AACCCAAGGA AGAGTCGGAA GAGGGGAACT CGGCTGAGTC GGCTGCAGAG 
GAGAGAGACC AGGATAAGAA ACGCAGAGTT GTAGACACAG AGAGTGGTGC TGCAGCTGCT 
GTGGAGAAAC TGGAAGAGGT AACAGCGGGA ACCCAGCTGG GTCCGGAAGA GCCATGTGAA 
CAGGAAGATG ACAACAGGAG TCTTCGACGT CACACCAGAG AGCTATCATT GAGGCGGAAA 
TCAAAGGAGG ATCCAGACAG AGAAGCAAGA CCGGAAACTC ACTTGGACGA GGACGAGGAC 
GGAAAAAAGG ATAAAAGAAG TTCCAGACCC AGGAGCCAGC CCAGAGATCC AGCTGCCAAA 
CGGAGACCCA AGGAAGCAGA GCCAGAGCAG GTAGCTCCAG AGACTCCCGA GGACAGAGAC 
GAGGATGAGA GGGAGGAGAA GAGACGAAAA ACGACACGTA AAAAACTGGA GTCACACACC 
GTTCCCGTTC AGAGCAGATC GGAGAGAAAA GCCGCTCAAA GCAAAAGTGT GATCCCGAAG 
ATCAACTCAC CAAAGTGCCC CGAGTGTGGC CAGCACCTAG ACGACCCTAA CCTGAAGTAC 
CAGCAGCACC CTGAGGATGC TGTGGATGAA CCCCAGATGT TGACCAGTGA GAAACTGTCC 
ATCTACGACT CCACCTCGAC CTGGTTTGAT ACTTATGAAG ATTCTCCCAT GCATAGGTTC 
ACTTCCTTCA GTGTGTACTG CAGTCGCGGG CACCTGTGTC CTGTCGACAC CGGTCTCATT 
GAGAAGAATG TAGAGCTCTA CTTTTCTGGG TGTGCCAAAG CAATTCATGA CGAGAATCCA 
TCTATGGAAG GTGGTATTAA TGGCAAAAAC CTCGGGCCAA TCAATCAGTG GTGGCTCAGT 
GGCTTTGATG GTGGCGAGAA GGTGCTCATT GGCTTCTCCA CTGCATTTGC TGAATACATT 
TTGATGGAGC CCAGCAAAGA GTATGAGCCA ATATTTGGGC TGATGCAGGA GAAAATTTAC 
ATCAGCAAGA TTGTTGTTGA GTTCCTGCAA AACAATCCTG ATGCTGTATA TGAAGACCTG 
ATCAATAAGA TTGAGACCAC TGTTCCTCCT TCTACCATTA ATGTGAACCG GTTCACAGAG 
GACTCCCTCT TACGCCACGC CCAGTTTGTA GTGAGCCAGG TAGAGAGTTA CGACGAAGCC 
AAGGACGATG ATGAGACCCC CATCTTCTTG TCTCCCTGTA TGAGAGCCCT GATCCATTTG 
GCTGGTGTCT CCCTGGGACA GAGGCGAGCA ACAAGGCGCG TCATGGGTGC TACCAAGGAG 
AAGGACAAAG CACCCACGAA AGCCACCACC ACCAAGCTGG TCTATCAGAT CTTTGACACT 
TTCTTCTCAG AGCAGATTGA GAAGTATGAT AAGGAGGACA AGGAGAATGC CATGAAGCGC 
CGCCGCTGTG GTGTCTGTGA GGTCTGTCAG CAGCCTGAGT GTGGGAAGTG CAAGGCGTGC 
AAAGATATGG TGAAGTTTGG TGGCACTGGA CGGAGTAAGC AGGCTTGCCT CAAGAGGAGG 
TGTCCTAACT TGGCGGTGAA GGAGGCAGAC GACGATGAAG AGGCTGATGA TGATGTGTCA 
GAGATGCCAT CACCCAAAAA GCTGCATCAG GGGAAGAAGA AGAAGCAGAA CAAGGACCGC 
ATCTCCTGGC TTGGGCAGCC TATGAAGATT GAAGAGAATA GAACTTACTA TCAGAAGGTG 
AGCATCGATG AGGAGATGCT AGAGGTGGGC GACTGCGTCT CGGTCATTCC AGATGATTCC 
TCCAAACCAC TCTATCTAGC CAGGGTCACA GCTCTGTGGG AAGACAAAAA TGGTCAGATG 
ATGTTCCATG CGCACTGGTT CTGCGCTGGG ACAGACACAG TCCTGGGAGC CACCTCCGAC 
CCCCTGGAAC TGTTCCTGGT GGGCGAGTGC GAAAACATGC AGCTTTCCTA CATCCACAGC 
AAGGTCAAGG TCATCTACAA AGCCCCTTCT GAAAACTGGG CCATGGAGGG AGGCACAGAC 
CCTGAGACCA CACTGCCTGG GGCTGAGGAT GGCAAGACTT ACTTCTTCCA GCTCTGGTAC 
AACCAGGAGT ACGCAAGGTT TGAATCCCCA CCCAAGACCC AGCCGACCGA GGACAACAAG 
CACAAGTTCT GCCTATCTTG TATCCGGCTG GCTGAGCTGA GACAAAAAGA AATGCCCAAG 
GTCCTGGAAC AAATTGAGGA GGTGGATGGC CGGGTCTACT GCAGTTCCAT CACCAAGAAT 
GGTGTTGTCT ACCGACTGGG TGACAGTGTG TACCTTCCTC CCGAGGCCTT TACTTTCAAC 
ATCAAAGTGG CTAGCCCCGT GAAACGCCCA AAGAAGGATC CTGTGAACGA GACCCTGTAC 
CCTGAGCACT ACCGCAAGTA TTCTGACTAC ATCAAGGGGA GCAACCTGGA TGCTCCAGAG 
CCCTATCGCA TCGGTCGGAT AAAAGAGATC CACTGTGGCA AGAAGAAAGG CAAGGTCAAC 
GAGGCAGACA TCAAGCTGAG GCTCTACAAG TTCTACAGGC CTGAGAATAC CCACAGGTCC 
TACAACGGAT CCTATCACAC TGACATCAAC ATGCTTTACT GGAGCGACGA GGAAGCTGTG 
GTGAACTTCA GCGACGTGCA GGGCCGCTGT ACCGTGGAGT ACGGGGAAGA CCTACTTGAG 
AGCATCCAGG ATTATTCACA AGGGGGCCCT GACCGCTTCT ACTTCCTCGA GGCCTACAAT 
TCAAAGACCA AGAACTTTGA AGACCCACCA AACCATGCCC GCAGCCCTGG GAACAAAGGG 
AAAGGGAAGG GGAAAGGGAA GGGGAAGGGG AAGCATCAGG TGTCAGAGCC CAAAGAGCCT 
GAGGCAGCCA TCAAACTGCC CAAGCTCCGG ACCCTGGATG TGTTTTCCGG CTGTGGAGGG 
TTATCGGAAG GATTCCACCA AGCAGGCATC TCGGAAACGC TGTGGGCCAT CGAGATGTGG 
GACCCGGCAG CCCAGGCATT TCGGCTGAAC AACCCCGGCA CCACAGTGTT CACAGAGGAC 
TGCAACGTGC TTCTTAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCTCT GGGCCAAAGG 
CTGCCACAGA AGGGCGATGT GGAGATGCTG TGTGGTGGGC CACCCTGCCA GGGCTTCAGT 
GGCATGAACC GCTTCAACTC CCGCACTTAC TCCAAGTTCA AAAACTCCCT AGTGGTCTCC 
TTCCTCAGCT ACTGTGACTA CTACCGGCCT CGGTTCTTCC TTCTGGAGAA CGTCAGGAAC 
TTCGTGTCCT ACAGACGCTC CATGGTGCTG AAGCTCACAC TGCGCTGCCT GGTCCGCATG 
GGCTACCAGT GCACCTTTGG TGTGCTCCAG GCTGGACAGT ATGGCGTGGC CCAGACACGA 
AGGAGGGCCA TCATCTTGGC TGCAGCCCCA GGAGAAAAGC TGCCTCTGTT CCCAGAGCCT 
CTGCATGTGT TTGCGCCCCG TGCCTGCCAG CTGAGCGTTG TGGTGGATGA CAAGAAGTTT 
GTTAGCAACA TAACGAGGCT GAGCTCGGGG CCCTTCCGAA CCATCACCGT GCGAGACACC 
ATGTCTGACC TCCCCGAGAT CCAGAATGGA GCCTCGAATT CTGAGATCCC CTACAATGGA 
GAGCCACTGT CCTGGTTCCA GAGGCAGCTG CGAGGATCAC ACTACCAGCC CATCCTCAGG 
GACCATATCT GCAAGGACAT GAGCCCACTG GTGGCTGCCC GCATGCGGCA CATCCCACTG 
TTCCCAGGAT CAGATTGGCG TGACCTGCCC AACATACAGG TGCGGCTGGG AGATGGCGTC 
ATAGCCCATA AGCTACAGTA CACCTTTCAT GATGTGAAAA ATGGCTACAG CAGCACCGGT 
GCCCTGCGTG GAGTCTGTTC CTGTGCAGAA GGCAAGGCCT GCGACCCTGA GTCCAGGCAA 
TTCAGCACCC TCATCCCCTG GTGCCTGCCG CACACTGGGA ACCGGCACAA CCACTGGGCT 
GGCCTCTACG GGCGTCTGGA GTGGGATGGC TTCTTCAGCA CCACTGTCAC CAACCCTGAG 
CCCATGGGCA AGCAGGGTCG GGTGCTCCAC CCGGAGCAGC ACCGGGTCGT GAGTGTTCGG 
GAATGTGCCC GCTCCCAGGG CTTTCCAGAT AGCTACCGGT TCTTCGGCAA CATCCTGGAC 
AGACACCGGC AGGTGGGTAA TGCTGTGCCA CCACCCCTGG CCAAAGCCAT TGGCCTGGAG 
ATTAAGCTCT GCCTGCTGTC CAGTGCTCGG GAGAGCGCAT CAGCTGCAGT TAAAGCAAAA 
GAGGAGGCTG CTACCAAGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnmt1 ( NM_001199433.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001186362.1 Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 4, mRNA.

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CloneID	OMu02259
Clone ID Related Accession (Same CDS sequence)	NM_001199433.1
Accession Version	NM_001199433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	DNA (cytosine-5)-methyltransferase 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF036009.1, CK636383.1, AF175432.1 and AK147143.1. Transcript Variant: This variant (4) uses an alternate first exon, which results in use of a downstream start codon compared to variant 1. The resulting protein (isoform 4) has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF175432.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCAGACT CAAATAGATC CCCAAGATCC AGGCCCAAGC CTCGGGGACC CAGGAGAAGC 
AAGTCGGACA GTGACACCCT TTCAGTTGAA ACTTCACCTA GTTCCGTGGC TACGAGGAGA 
ACCACCAGGC AGACCACCAT CACGGCTCAC TTCACGAAGG GCCCCACTAA ACGGAAACCC 
AAGGAAGAGT CGGAAGAGGG GAACTCGGCT GAGTCGGCTG CAGAGGAGAG AGACCAGGAT 
AAGAAACGCA GAGTTGTAGA CACAGAGAGT GGTGCTGCAG CTGCTGTGGA GAAACTGGAA 
GAGGTAACAG CGGGAACCCA GCTGGGTCCG GAAGAGCCAT GTGAACAGGA AGATGACAAC 
AGGAGTCTTC GACGTCACAC CAGAGAGCTA TCATTGAGGC GGAAATCAAA GGAGGATCCA 
GACAGAGAAG CAAGACCGGA AACTCACTTG GACGAGGACG AGGACGGAAA AAAGGATAAA 
AGAAGTTCCA GACCCAGGAG CCAGCCCAGA GATCCAGCTG CCAAACGGAG ACCCAAGGAA 
GCAGAGCCAG AGCAGGTAGC TCCAGAGACT CCCGAGGACA GAGACGAGGA TGAGAGGGAG 
GAGAAGAGAC GAAAAACGAC ACGTAAAAAA CTGGAGTCAC ACACCGTTCC CGTTCAGAGC 
AGATCGGAGA GAAAAGCCGC TCAAAGCAAA AGTGTGATCC CGAAGATCAA CTCACCAAAG 
TGCCCCGAGT GTGGCCAGCA CCTAGACGAC CCTAACCTGA AGTACCAGCA GCACCCTGAG 
GATGCTGTGG ATGAACCCCA GATGTTGACC AGTGAGAAAC TGTCCATCTA CGACTCCACC 
TCGACCTGGT TTGATACTTA TGAAGATTCT CCCATGCATA GGTTCACTTC CTTCAGTGTG 
TACTGCAGTC GCGGGCACCT GTGTCCTGTC GACACCGGTC TCATTGAGAA GAATGTAGAG 
CTCTACTTTT CTGGGTGTGC CAAAGCAATT CATGACGAGA ATCCATCTAT GGAAGGTGGT 
ATTAATGGCA AAAACCTCGG GCCAATCAAT CAGTGGTGGC TCAGTGGCTT TGATGGTGGC 
GAGAAGGTGC TCATTGGCTT CTCCACTGCA TTTGCTGAAT ACATTTTGAT GGAGCCCAGC 
AAAGAGTATG AGCCAATATT TGGGCTGATG CAGGAGAAAA TTTACATCAG CAAGATTGTT 
GTTGAGTTCC TGCAAAACAA TCCTGATGCT GTATATGAAG ACCTGATCAA TAAGATTGAG 
ACCACTGTTC CTCCTTCTAC CATTAATGTG AACCGGTTCA CAGAGGACTC CCTCTTACGC 
CACGCCCAGT TTGTAGTGAG CCAGGTAGAG AGTTACGACG AAGCCAAGGA CGATGATGAG 
ACCCCCATCT TCTTGTCTCC CTGTATGAGA GCCCTGATCC ATTTGGCTGG TGTCTCCCTG 
GGACAGAGGC GAGCAACAAG GCGCGTCATG GGTGCTACCA AGGAGAAGGA CAAAGCACCC 
ACGAAAGCCA CCACCACCAA GCTGGTCTAT CAGATCTTTG ACACTTTCTT CTCAGAGCAG 
ATTGAGAAGT ATGATAAGGA GGACAAGGAG AATGCCATGA AGCGCCGCCG CTGTGGTGTC 
TGTGAGGTCT GTCAGCAGCC TGAGTGTGGG AAGTGCAAGG CGTGCAAAGA TATGGTGAAG 
TTTGGTGGCA CTGGACGGAG TAAGCAGGCT TGCCTCAAGA GGAGGTGTCC TAACTTGGCG 
GTGAAGGAGG CAGACGACGA TGAAGAGGCT GATGATGATG TGTCAGAGAT GCCATCACCC 
AAAAAGCTGC ATCAGGGGAA GAAGAAGAAG CAGAACAAGG ACCGCATCTC CTGGCTTGGG 
CAGCCTATGA AGATTGAAGA GAATAGAACT TACTATCAGA AGGTGAGCAT CGATGAGGAG 
ATGCTAGAGG TGGGCGACTG CGTCTCGGTC ATTCCAGATG ATTCCTCCAA ACCACTCTAT 
CTAGCCAGGG TCACAGCTCT GTGGGAAGAC AAAAATGGTC AGATGATGTT CCATGCGCAC 
TGGTTCTGCG CTGGGACAGA CACAGTCCTG GGAGCCACCT CCGACCCCCT GGAACTGTTC 
CTGGTGGGCG AGTGCGAAAA CATGCAGCTT TCCTACATCC ACAGCAAGGT CAAGGTCATC 
TACAAAGCCC CTTCTGAAAA CTGGGCCATG GAGGGAGGCA CAGACCCTGA GACCACACTG 
CCTGGGGCTG AGGATGGCAA GACTTACTTC TTCCAGCTCT GGTACAACCA GGAGTACGCA 
AGGTTTGAAT CCCCACCCAA GACCCAGCCG ACCGAGGACA ACAAGCACAA GTTCTGCCTA 
TCTTGTATCC GGCTGGCTGA GCTGAGACAA AAAGAAATGC CCAAGGTCCT GGAACAAATT 
GAGGAGGTGG ATGGCCGGGT CTACTGCAGT TCCATCACCA AGAATGGTGT TGTCTACCGA 
CTGGGTGACA GTGTGTACCT TCCTCCCGAG GCCTTTACTT TCAACATCAA AGTGGCTAGC 
CCCGTGAAAC GCCCAAAGAA GGATCCTGTG AACGAGACCC TGTACCCTGA GCACTACCGC 
AAGTATTCTG ACTACATCAA GGGGAGCAAC CTGGATGCTC CAGAGCCCTA TCGCATCGGT 
CGGATAAAAG AGATCCACTG TGGCAAGAAG AAAGGCAAGG TCAACGAGGC AGACATCAAG 
CTGAGGCTCT ACAAGTTCTA CAGGCCTGAG AATACCCACA GGTCCTACAA CGGATCCTAT 
CACACTGACA TCAACATGCT TTACTGGAGC GACGAGGAAG CTGTGGTGAA CTTCAGCGAC 
GTGCAGGGCC GCTGTACCGT GGAGTACGGG GAAGACCTAC TTGAGAGCAT CCAGGATTAT 
TCACAAGGGG GCCCTGACCG CTTCTACTTC CTCGAGGCCT ACAATTCAAA GACCAAGAAC 
TTTGAAGACC CACCAAACCA TGCCCGCAGC CCTGGGAACA AAGGGAAAGG GAAGGGGAAA 
GGGAAGGGGA AGGGGAAGCA TCAGGTGTCA GAGCCCAAAG AGCCTGAGGC AGCCATCAAA 
CTGCCCAAGC TCCGGACCCT GGATGTGTTT TCCGGCTGTG GAGGGTTATC GGAAGGATTC 
CACCAAGCAG GCATCTCGGA AACGCTGTGG GCCATCGAGA TGTGGGACCC GGCAGCCCAG 
GCATTTCGGC TGAACAACCC CGGCACCACA GTGTTCACAG AGGACTGCAA CGTGCTTCTT 
AAGCTGGTCA TGGCTGGGGA GGTGACCAAC TCTCTGGGCC AAAGGCTGCC ACAGAAGGGC 
GATGTGGAGA TGCTGTGTGG TGGGCCACCC TGCCAGGGCT TCAGTGGCAT GAACCGCTTC 
AACTCCCGCA CTTACTCCAA GTTCAAAAAC TCCCTAGTGG TCTCCTTCCT CAGCTACTGT 
GACTACTACC GGCCTCGGTT CTTCCTTCTG GAGAACGTCA GGAACTTCGT GTCCTACAGA 
CGCTCCATGG TGCTGAAGCT CACACTGCGC TGCCTGGTCC GCATGGGCTA CCAGTGCACC 
TTTGGTGTGC TCCAGGCTGG ACAGTATGGC GTGGCCCAGA CACGAAGGAG GGCCATCATC 
TTGGCTGCAG CCCCAGGAGA AAAGCTGCCT CTGTTCCCAG AGCCTCTGCA TGTGTTTGCG 
CCCCGTGCCT GCCAGCTGAG CGTTGTGGTG GATGACAAGA AGTTTGTTAG CAACATAACG 
AGGCTGAGCT CGGGGCCCTT CCGAACCATC ACCGTGCGAG ACACCATGTC TGACCTCCCC 
GAGATCCAGA ATGGAGCCTC GAATTCTGAG ATCCCCTACA ATGGAGAGCC ACTGTCCTGG 
TTCCAGAGGC AGCTGCGAGG ATCACACTAC CAGCCCATCC TCAGGGACCA TATCTGCAAG 
GACATGAGCC CACTGGTGGC TGCCCGCATG CGGCACATCC CACTGTTCCC AGGATCAGAT 
TGGCGTGACC TGCCCAACAT ACAGGTGCGG CTGGGAGATG GCGTCATAGC CCATAAGCTA 
CAGTACACCT TTCATGATGT GAAAAATGGC TACAGCAGCA CCGGTGCCCT GCGTGGAGTC 
TGTTCCTGTG CAGAAGGCAA GGCCTGCGAC CCTGAGTCCA GGCAATTCAG CACCCTCATC 
CCCTGGTGCC TGCCGCACAC TGGGAACCGG CACAACCACT GGGCTGGCCT CTACGGGCGT 
CTGGAGTGGG ATGGCTTCTT CAGCACCACT GTCACCAACC CTGAGCCCAT GGGCAAGCAG 
GGTCGGGTGC TCCACCCGGA GCAGCACCGG GTCGTGAGTG TTCGGGAATG TGCCCGCTCC 
CAGGGCTTTC CAGATAGCTA CCGGTTCTTC GGCAACATCC TGGACAGACA CCGGCAGGTG 
GGTAATGCTG TGCCACCACC CCTGGCCAAA GCCATTGGCC TGGAGATTAA GCTCTGCCTG 
CTGTCCAGTG CTCGGGAGAG CGCATCAGCT GCAGTTAAAG CAAAAGAGGA GGCTGCTACC 
AAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186362.1
CDS	1053..5561
Translation	MADSNRSPRSRPKPRGPRRSKSDSDTLSVETSPSSVATRRTTRQTTITAHFTKGPTKRKPKEESEEGNSAESAAEERDQDKKRRVVDTESGAAAAVEKLEEVTAGTQLGPEEPCEQEDDNRSLRRHTRELSLRRKSKEDPDREARPETHLDEDEDGKKDKRSSRPRSQPRDPAAKRRPKEAEPEQVAPETPEDRDEDEREEKRRKTTRKKLESHTVPVQSRSERKAAQSKSVIPKINSPKCPECGQHLDDPNLKYQQHPEDAVDEPQMLTSEKLSIYDSTSTWFDTYEDSPMHRFTSFSVYCSRGHLCPVDTGLIEKNVELYFSGCAKAIHDENPSMEGGINGKNLGPINQWWLSGFDGGEKVLIGFSTAFAEYILMEPSKEYEPIFGLMQEKIYISKIVVEFLQNNPDAVYEDLINKIETTVPPSTINVNRFTEDSLLRHAQFVVSQVESYDEAKDDDETPIFLSPCMRALIHLAGVSLGQRRATRRVMGATKEKDKAPTKATTTKLVYQIFDTFFSEQIEKYDKEDKENAMKRRRCGVCEVCQQPECGKCKACKDMVKFGGTGRSKQACLKRRCPNLAVKEADDDEEADDDVSEMPSPKKLHQGKKKKQNKDRISWLGQPMKIEENRTYYQKVSIDEEMLEVGDCVSVIPDDSSKPLYLARVTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYIHSKVKVIYKAPSENWAMEGGTDPETTLPGAEDGKTYFFQLWYNQEYARFESPPKTQPTEDNKHKFCLSCIRLAELRQKEMPKVLEQIEEVDGRVYCSSITKNGVVYRLGDSVYLPPEAFTFNIKVASPVKRPKKDPVNETLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPENTHRSYNGSYHTDINMLYWSDEEAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSKTKNFEDPPNHARSPGNKGKGKGKGKGKGKHQVSEPKEPEAAIKLPKLRTLDVFSGCGGLSEGFHQAGISETLWAIEMWDPAAQAFRLNNPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEIQNGASNSEIPYNGEPLSWFQRQLRGSHYQPILRDHICKDMSPLVAARMRHIPLFPGSDWRDLPNIQVRLGDGVIAHKLQYTFHDVKNGYSSTGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDSYRFFGNILDRHRQVGNAVPPPLAKAIGLEIKLCLLSSARESASAAVKAKEEAATKD

Target ORF information:

RefSeq Version	NM_001199433.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199433.1

ORF Insert Sequence:

ATGGCAGACT CAAATAGATC CCCAAGATCC AGGCCCAAGC CTCGGGGACC CAGGAGAAGC 
AAGTCGGACA GTGACACCCT TTCAGTTGAA ACTTCACCTA GTTCCGTGGC TACGAGGAGA 
ACCACCAGGC AGACCACCAT CACGGCTCAC TTCACGAAGG GCCCCACTAA ACGGAAACCC 
AAGGAAGAGT CGGAAGAGGG GAACTCGGCT GAGTCGGCTG CAGAGGAGAG AGACCAGGAT 
AAGAAACGCA GAGTTGTAGA CACAGAGAGT GGTGCTGCAG CTGCTGTGGA GAAACTGGAA 
GAGGTAACAG CGGGAACCCA GCTGGGTCCG GAAGAGCCAT GTGAACAGGA AGATGACAAC 
AGGAGTCTTC GACGTCACAC CAGAGAGCTA TCATTGAGGC GGAAATCAAA GGAGGATCCA 
GACAGAGAAG CAAGACCGGA AACTCACTTG GACGAGGACG AGGACGGAAA AAAGGATAAA 
AGAAGTTCCA GACCCAGGAG CCAGCCCAGA GATCCAGCTG CCAAACGGAG ACCCAAGGAA 
GCAGAGCCAG AGCAGGTAGC TCCAGAGACT CCCGAGGACA GAGACGAGGA TGAGAGGGAG 
GAGAAGAGAC GAAAAACGAC ACGTAAAAAA CTGGAGTCAC ACACCGTTCC CGTTCAGAGC 
AGATCGGAGA GAAAAGCCGC TCAAAGCAAA AGTGTGATCC CGAAGATCAA CTCACCAAAG 
TGCCCCGAGT GTGGCCAGCA CCTAGACGAC CCTAACCTGA AGTACCAGCA GCACCCTGAG 
GATGCTGTGG ATGAACCCCA GATGTTGACC AGTGAGAAAC TGTCCATCTA CGACTCCACC 
TCGACCTGGT TTGATACTTA TGAAGATTCT CCCATGCATA GGTTCACTTC CTTCAGTGTG 
TACTGCAGTC GCGGGCACCT GTGTCCTGTC GACACCGGTC TCATTGAGAA GAATGTAGAG 
CTCTACTTTT CTGGGTGTGC CAAAGCAATT CATGACGAGA ATCCATCTAT GGAAGGTGGT 
ATTAATGGCA AAAACCTCGG GCCAATCAAT CAGTGGTGGC TCAGTGGCTT TGATGGTGGC 
GAGAAGGTGC TCATTGGCTT CTCCACTGCA TTTGCTGAAT ACATTTTGAT GGAGCCCAGC 
AAAGAGTATG AGCCAATATT TGGGCTGATG CAGGAGAAAA TTTACATCAG CAAGATTGTT 
GTTGAGTTCC TGCAAAACAA TCCTGATGCT GTATATGAAG ACCTGATCAA TAAGATTGAG 
ACCACTGTTC CTCCTTCTAC CATTAATGTG AACCGGTTCA CAGAGGACTC CCTCTTACGC 
CACGCCCAGT TTGTAGTGAG CCAGGTAGAG AGTTACGACG AAGCCAAGGA CGATGATGAG 
ACCCCCATCT TCTTGTCTCC CTGTATGAGA GCCCTGATCC ATTTGGCTGG TGTCTCCCTG 
GGACAGAGGC GAGCAACAAG GCGCGTCATG GGTGCTACCA AGGAGAAGGA CAAAGCACCC 
ACGAAAGCCA CCACCACCAA GCTGGTCTAT CAGATCTTTG ACACTTTCTT CTCAGAGCAG 
ATTGAGAAGT ATGATAAGGA GGACAAGGAG AATGCCATGA AGCGCCGCCG CTGTGGTGTC 
TGTGAGGTCT GTCAGCAGCC TGAGTGTGGG AAGTGCAAGG CGTGCAAAGA TATGGTGAAG 
TTTGGTGGCA CTGGACGGAG TAAGCAGGCT TGCCTCAAGA GGAGGTGTCC TAACTTGGCG 
GTGAAGGAGG CAGACGACGA TGAAGAGGCT GATGATGATG TGTCAGAGAT GCCATCACCC 
AAAAAGCTGC ATCAGGGGAA GAAGAAGAAG CAGAACAAGG ACCGCATCTC CTGGCTTGGG 
CAGCCTATGA AGATTGAAGA GAATAGAACT TACTATCAGA AGGTGAGCAT CGATGAGGAG 
ATGCTAGAGG TGGGCGACTG CGTCTCGGTC ATTCCAGATG ATTCCTCCAA ACCACTCTAT 
CTAGCCAGGG TCACAGCTCT GTGGGAAGAC AAAAATGGTC AGATGATGTT CCATGCGCAC 
TGGTTCTGCG CTGGGACAGA CACAGTCCTG GGAGCCACCT CCGACCCCCT GGAACTGTTC 
CTGGTGGGCG AGTGCGAAAA CATGCAGCTT TCCTACATCC ACAGCAAGGT CAAGGTCATC 
TACAAAGCCC CTTCTGAAAA CTGGGCCATG GAGGGAGGCA CAGACCCTGA GACCACACTG 
CCTGGGGCTG AGGATGGCAA GACTTACTTC TTCCAGCTCT GGTACAACCA GGAGTACGCA 
AGGTTTGAAT CCCCACCCAA GACCCAGCCG ACCGAGGACA ACAAGCACAA GTTCTGCCTA 
TCTTGTATCC GGCTGGCTGA GCTGAGACAA AAAGAAATGC CCAAGGTCCT GGAACAAATT 
GAGGAGGTGG ATGGCCGGGT CTACTGCAGT TCCATCACCA AGAATGGTGT TGTCTACCGA 
CTGGGTGACA GTGTGTACCT TCCTCCCGAG GCCTTTACTT TCAACATCAA AGTGGCTAGC 
CCCGTGAAAC GCCCAAAGAA GGATCCTGTG AACGAGACCC TGTACCCTGA GCACTACCGC 
AAGTATTCTG ACTACATCAA GGGGAGCAAC CTGGATGCTC CAGAGCCCTA TCGCATCGGT 
CGGATAAAAG AGATCCACTG TGGCAAGAAG AAAGGCAAGG TCAACGAGGC AGACATCAAG 
CTGAGGCTCT ACAAGTTCTA CAGGCCTGAG AATACCCACA GGTCCTACAA CGGATCCTAT 
CACACTGACA TCAACATGCT TTACTGGAGC GACGAGGAAG CTGTGGTGAA CTTCAGCGAC 
GTGCAGGGCC GCTGTACCGT GGAGTACGGG GAAGACCTAC TTGAGAGCAT CCAGGATTAT 
TCACAAGGGG GCCCTGACCG CTTCTACTTC CTCGAGGCCT ACAATTCAAA GACCAAGAAC 
TTTGAAGACC CACCAAACCA TGCCCGCAGC CCTGGGAACA AAGGGAAAGG GAAGGGGAAA 
GGGAAGGGGA AGGGGAAGCA TCAGGTGTCA GAGCCCAAAG AGCCTGAGGC AGCCATCAAA 
CTGCCCAAGC TCCGGACCCT GGATGTGTTT TCCGGCTGTG GAGGGTTATC GGAAGGATTC 
CACCAAGCAG GCATCTCGGA AACGCTGTGG GCCATCGAGA TGTGGGACCC GGCAGCCCAG 
GCATTTCGGC TGAACAACCC CGGCACCACA GTGTTCACAG AGGACTGCAA CGTGCTTCTT 
AAGCTGGTCA TGGCTGGGGA GGTGACCAAC TCTCTGGGCC AAAGGCTGCC ACAGAAGGGC 
GATGTGGAGA TGCTGTGTGG TGGGCCACCC TGCCAGGGCT TCAGTGGCAT GAACCGCTTC 
AACTCCCGCA CTTACTCCAA GTTCAAAAAC TCCCTAGTGG TCTCCTTCCT CAGCTACTGT 
GACTACTACC GGCCTCGGTT CTTCCTTCTG GAGAACGTCA GGAACTTCGT GTCCTACAGA 
CGCTCCATGG TGCTGAAGCT CACACTGCGC TGCCTGGTCC GCATGGGCTA CCAGTGCACC 
TTTGGTGTGC TCCAGGCTGG ACAGTATGGC GTGGCCCAGA CACGAAGGAG GGCCATCATC 
TTGGCTGCAG CCCCAGGAGA AAAGCTGCCT CTGTTCCCAG AGCCTCTGCA TGTGTTTGCG 
CCCCGTGCCT GCCAGCTGAG CGTTGTGGTG GATGACAAGA AGTTTGTTAG CAACATAACG 
AGGCTGAGCT CGGGGCCCTT CCGAACCATC ACCGTGCGAG ACACCATGTC TGACCTCCCC 
GAGATCCAGA ATGGAGCCTC GAATTCTGAG ATCCCCTACA ATGGAGAGCC ACTGTCCTGG 
TTCCAGAGGC AGCTGCGAGG ATCACACTAC CAGCCCATCC TCAGGGACCA TATCTGCAAG 
GACATGAGCC CACTGGTGGC TGCCCGCATG CGGCACATCC CACTGTTCCC AGGATCAGAT 
TGGCGTGACC TGCCCAACAT ACAGGTGCGG CTGGGAGATG GCGTCATAGC CCATAAGCTA 
CAGTACACCT TTCATGATGT GAAAAATGGC TACAGCAGCA CCGGTGCCCT GCGTGGAGTC 
TGTTCCTGTG CAGAAGGCAA GGCCTGCGAC CCTGAGTCCA GGCAATTCAG CACCCTCATC 
CCCTGGTGCC TGCCGCACAC TGGGAACCGG CACAACCACT GGGCTGGCCT CTACGGGCGT 
CTGGAGTGGG ATGGCTTCTT CAGCACCACT GTCACCAACC CTGAGCCCAT GGGCAAGCAG 
GGTCGGGTGC TCCACCCGGA GCAGCACCGG GTCGTGAGTG TTCGGGAATG TGCCCGCTCC 
CAGGGCTTTC CAGATAGCTA CCGGTTCTTC GGCAACATCC TGGACAGACA CCGGCAGGTG 
GGTAATGCTG TGCCACCACC CCTGGCCAAA GCCATTGGCC TGGAGATTAA GCTCTGCCTG 
CTGTCCAGTG CTCGGGAGAG CGCATCAGCT GCAGTTAAAG CAAAAGAGGA GGCTGCTACC 
AAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnmt1 ( XM_011242393.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240695.1 Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu67558
Clone ID Related Accession (Same CDS sequence)	XM_011242393.2
Accession Version	XM_011242393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA (cytosine-5)-methyltransferase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACT CAAATAGATC CCCAAGATCC AGGCCCAAGC CTCGGGGACC CAGGAGAAGC 
AAGTCGGACA GTGACACCCT TTGTAAGGAC ACGAGACATA CTGCAGTTGA AACTTCACCT 
AGTTCCGTGG CTACGAGGAG AACCACCAGG CAGACCACCA TCACGGCTCA CTTCACGAAG 
GGCCCCACTA AACGGAAACC CAAGGAAGAG TCGGAAGAGG GGAACTCGGC TGAGTCGGCT 
GCAGAGGAGA GAGACCAGGA TAAGAAACGC AGAGTTGTAG ACACAGAGAG TGGTGCTGCA 
GCTGCTGTGG AGAAACTGGA AGAGGTAACA GCGGGAACCC AGCTGGGTCC GGAAGAGCCA 
TGTGAACAGG AAGATGACAA CAGGAGTCTT CGACGTCACA CCAGAGAGCT ATCATTGAGG 
CGGAAATCAA AGGAGGATCC AGACAGAGAA GCAAGACCGG AAACTCACTT GGACGAGGAC 
GAGGACGGAA AAAAGGATAA AAGAAGTTCC AGACCCAGGA GCCAGCCCAG AGATCCAGCT 
GCCAAACGGA GACCCAAGGA AGCAGAGCCA GAGCAGGTAG CTCCAGAGAC TCCCGAGGAC 
AGAGACGAGG ATGAGAGGGA GGAGAAGAGA CGAAAAACGA CACGTAAAAA ACTGGAGTCA 
CACACCGTTC CCGTTCAGAG CAGATCGGAG AGAAAAGCCG CTCAAAGCAA AAGTGTGATC 
CCGAAGATCA ACTCACCAAA GTGCCCCGAG TGTGGCCAGC ACCTAGACGA CCCTAACCTG 
AAGTACCAGC AGCACCCTGA GGATGCTGTG GATGAACCCC AGATGTTGAC CAGTGAGAAA 
CTGTCCATCT ACGACTCCAC CTCGACCTGG TTTGATACTT ATGAAGATTC TCCCATGCAT 
AGGTTCACTT CCTTCAGTGT GTACTGCAGT CGCGGGCACC TGTGTCCTGT CGACACCGGT 
CTCATTGAGA AGAATGTAGA GCTCTACTTT TCTGGGTGTG CCAAAGCAAT TCATGACGAG 
AATCCATCTA TGGAAGGTGG TATTAATGGC AAAAACCTCG GGCCAATCAA TCAGTGGTGG 
CTCAGTGGCT TTGATGGTGG CGAGAAGGTG CTCATTGGCT TCTCCACTGC ATTTGCTGAA 
TACATTTTGA TGGAGCCCAG CAAAGAGTAT GAGCCAATAT TTGGGCTGAT GCAGGAGAAA 
ATTTACATCA GCAAGATTGT TGTTGAGTTC CTGCAAAACA ATCCTGATGC TGTATATGAA 
GACCTGATCA ATAAGATTGA GACCACTGTT CCTCCTTCTA CCATTAATGT GAACCGGTTC 
ACAGAGGACT CCCTCTTACG CCACGCCCAG TTTGTAGTGA GCCAGGTAGA GAGTTACGAC 
GAAGCCAAGG ACGATGATGA GACCCCCATC TTCTTGTCTC CCTGTATGAG AGCCCTGATC 
CATTTGGCTG GTGTCTCCCT GGGACAGAGG CGAGCAACAA GGCGCGTCAT GGGTGCTACC 
AAGGAGAAGG ACAAAGCACC CACGAAAGCC ACCACCACCA AGCTGGTCTA TCAGATCTTT 
GACACTTTCT TCTCAGAGCA GATTGAGAAG TATGATAAGG AGGACAAGGA GAATGCCATG 
AAGCGCCGCC GCTGTGGTGT CTGTGAGGTC TGTCAGCAGC CTGAGTGTGG GAAGTGCAAG 
GCGTGCAAAG ATATGGTGAA GTTTGGTGGC ACTGGACGGA GTAAGCAGGC TTGCCTCAAG 
AGGAGGTGTC CTAACTTGGC GGTGAAGGAG GCAGACGACG ATGAAGAGGC TGATGATGAT 
GTGTCAGAGA TGCCATCACC CAAAAAGCTG CATCAGGGGA AGAAGAAGAA GCAGAACAAG 
GACCGCATCT CCTGGCTTGG GCAGCCTATG AAGATTGAAG AGAATAGAAC TTACTATCAG 
AAGGTGAGCA TCGATGAGGA GATGCTAGAG GTGGGCGACT GCGTCTCGGT CATTCCAGAT 
GATTCCTCCA AACCACTCTA TCTAGCCAGG GTCACAGCTC TGTGGGAAGA CAAAAATGGT 
CAGATGATGT TCCATGCGCA CTGGTTCTGC GCTGGGACAG ACACAGTCCT GGGAGCCACC 
TCCGACCCCC TGGAACTGTT CCTGGTGGGC GAGTGCGAAA ACATGCAGCT TTCCTACATC 
CACAGCAAGG TCAAGGTCAT CTACAAAGCC CCTTCTGAAA ACTGGGCCAT GGAGGGAGGC 
ACAGACCCTG AGACCACACT GCCTGGGGCT GAGGATGGCA AGACTTACTT CTTCCAGCTC 
TGGTACAACC AGGAGTACGC AAGGTTTGAA TCCCCACCCA AGACCCAGCC GACCGAGGAC 
AACAAGCACA AGTTCTGCCT ATCTTGTATC CGGCTGGCTG AGCTGAGACA AAAAGAAATG 
CCCAAGGTCC TGGAACAAAT TGAGGAGGTG GATGGCCGGG TCTACTGCAG TTCCATCACC 
AAGAATGGTG TTGTCTACCG ACTGGGTGAC AGTGTGTACC TTCCTCCCGA GGCCTTTACT 
TTCAACATCA AAGTGGCTAG CCCCGTGAAA CGCCCAAAGA AGGATCCTGT GAACGAGACC 
CTGTACCCTG AGCACTACCG CAAGTATTCT GACTACATCA AGGGGAGCAA CCTGGATGCT 
CCAGAGCCCT ATCGCATCGG TCGGATAAAA GAGATCCACT GTGGCAAGAA GAAAGGCAAG 
GTCAACGAGG CAGACATCAA GCTGAGGCTC TACAAGTTCT ACAGGCCTGA GAATACCCAC 
AGGTCCTACA ACGGATCCTA TCACACTGAC ATCAACATGC TTTACTGGAG CGACGAGGAA 
GCTGTGGTGA ACTTCAGCGA CGTGCAGGGC CGCTGTACCG TGGAGTACGG GGAAGACCTA 
CTTGAGAGCA TCCAGGATTA TTCACAAGGG GGCCCTGACC GCTTCTACTT CCTCGAGGCC 
TACAATTCAA AGACCAAGAA CTTTGAAGAC CCACCAAACC ATGCCCGCAG CCCTGGGAAC 
AAAGGGAAAG GGAAGGGGAA AGGGAAGGGG AAGGGGAAGC ATCAGGTGTC AGAGCCCAAA 
GAGCCTGAGG CAGCCATCAA ACTGCCCAAG CTCCGGACCC TGGATGTGTT TTCCGGCTGT 
GGAGGGTTAT CGGAAGGATT CCACCAAGCA GGCATCTCGG AAACGCTGTG GGCCATCGAG 
ATGTGGGACC CGGCAGCCCA GGCATTTCGG CTGAACAACC CCGGCACCAC AGTGTTCACA 
GAGGACTGCA ACGTGCTTCT TAAGCTGGTC ATGGCTGGGG AGGTGACCAA CTCTCTGGGC 
CAAAGGCTGC CACAGAAGGG CGATGTGGAG ATGCTGTGTG GTGGGCCACC CTGCCAGGGC 
TTCAGTGGCA TGAACCGCTT CAACTCCCGC ACTTACTCCA AGTTCAAAAA CTCCCTAGTG 
GTCTCCTTCC TCAGCTACTG TGACTACTAC CGGCCTCGGT TCTTCCTTCT GGAGAACGTC 
AGGAACTTCG TGTCCTACAG ACGCTCCATG GTGCTGAAGC TCACACTGCG CTGCCTGGTC 
CGCATGGGCT ACCAGTGCAC CTTTGGTGTG CTCCAGGCTG GACAGTATGG CGTGGCCCAG 
ACACGAAGGA GGGCCATCAT CTTGGCTGCA GCCCCAGGAG AAAAGCTGCC TCTGTTCCCA 
GAGCCTCTGC ATGTGTTTGC GCCCCGTGCC TGCCAGCTGA GCGTTGTGGT GGATGACAAG 
AAGTTTGTTA GCAACATAAC GAGGCTGAGC TCGGGGCCCT TCCGAACCAT CACCGTGCGA 
GACACCATGT CTGACCTCCC CGAGATCCAG AATGGAGCCT CGAATTCTGA GATCCCCTAC 
AATGGAGAGC CACTGTCCTG GTTCCAGAGG CAGCTGCGAG GATCACACTA CCAGCCCATC 
CTCAGGGACC ATATCTGCAA GGACATGAGC CCACTGGTGG CTGCCCGCAT GCGGCACATC 
CCACTGTTCC CAGGATCAGA TTGGCGTGAC CTGCCCAACA TACAGGTGCG GCTGGGAGAT 
GGCGTCATAG CCCATAAGCT ACAGTACACC TTTCATGATG TGAAAAATGG CTACAGCAGC 
ACCGGTGCCC TGCGTGGAGT CTGTTCCTGT GCAGAAGGCA AGGCCTGCGA CCCTGAGTCC 
AGGCAATTCA GCACCCTCAT CCCCTGGTGC CTGCCGCACA CTGGGAACCG GCACAACCAC 
TGGGCTGGCC TCTACGGGCG TCTGGAGTGG GATGGCTTCT TCAGCACCAC TGTCACCAAC 
CCTGAGCCCA TGGGCAAGCA GGGTCGGGTG CTCCACCCGG AGCAGCACCG GGTCGTGAGT 
GTTCGGGAAT GTGCCCGCTC CCAGGGCTTT CCAGATAGCT ACCGGTTCTT CGGCAACATC 
CTGGACAGAC ACCGGCAGGT GGGTAATGCT GTGCCACCAC CCCTGGCCAA AGCCATTGGC 
CTGGAGATTA AGCTCTGCCT GCTGTCCAGT GCTCGGGAGA GCGCATCAGC TGCAGTTAAA 
GCAAAAGAGG AGGCTGCTAC CAAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240695.1
CDS	339..4868
Translation	MADSNRSPRSRPKPRGPRRSKSDSDTLCKDTRHTAVETSPSSVATRRTTRQTTITAHFTKGPTKRKPKEESEEGNSAESAAEERDQDKKRRVVDTESGAAAAVEKLEEVTAGTQLGPEEPCEQEDDNRSLRRHTRELSLRRKSKEDPDREARPETHLDEDEDGKKDKRSSRPRSQPRDPAAKRRPKEAEPEQVAPETPEDRDEDEREEKRRKTTRKKLESHTVPVQSRSERKAAQSKSVIPKINSPKCPECGQHLDDPNLKYQQHPEDAVDEPQMLTSEKLSIYDSTSTWFDTYEDSPMHRFTSFSVYCSRGHLCPVDTGLIEKNVELYFSGCAKAIHDENPSMEGGINGKNLGPINQWWLSGFDGGEKVLIGFSTAFAEYILMEPSKEYEPIFGLMQEKIYISKIVVEFLQNNPDAVYEDLINKIETTVPPSTINVNRFTEDSLLRHAQFVVSQVESYDEAKDDDETPIFLSPCMRALIHLAGVSLGQRRATRRVMGATKEKDKAPTKATTTKLVYQIFDTFFSEQIEKYDKEDKENAMKRRRCGVCEVCQQPECGKCKACKDMVKFGGTGRSKQACLKRRCPNLAVKEADDDEEADDDVSEMPSPKKLHQGKKKKQNKDRISWLGQPMKIEENRTYYQKVSIDEEMLEVGDCVSVIPDDSSKPLYLARVTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYIHSKVKVIYKAPSENWAMEGGTDPETTLPGAEDGKTYFFQLWYNQEYARFESPPKTQPTEDNKHKFCLSCIRLAELRQKEMPKVLEQIEEVDGRVYCSSITKNGVVYRLGDSVYLPPEAFTFNIKVASPVKRPKKDPVNETLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPENTHRSYNGSYHTDINMLYWSDEEAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSKTKNFEDPPNHARSPGNKGKGKGKGKGKGKHQVSEPKEPEAAIKLPKLRTLDVFSGCGGLSEGFHQAGISETLWAIEMWDPAAQAFRLNNPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEIQNGASNSEIPYNGEPLSWFQRQLRGSHYQPILRDHICKDMSPLVAARMRHIPLFPGSDWRDLPNIQVRLGDGVIAHKLQYTFHDVKNGYSSTGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDSYRFFGNILDRHRQVGNAVPPPLAKAIGLEIKLCLLSSARESASAAVKAKEEAATKD

Target ORF information:

RefSeq Version	XM_011242393.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242393.2

ORF Insert Sequence:

ATGGCAGACT CAAATAGATC CCCAAGATCC AGGCCCAAGC CTCGGGGACC CAGGAGAAGC 
AAGTCGGACA GTGACACCCT TTGTAAGGAC ACGAGACATA CTGCAGTTGA AACTTCACCT 
AGTTCCGTGG CTACGAGGAG AACCACCAGG CAGACCACCA TCACGGCTCA CTTCACGAAG 
GGCCCCACTA AACGGAAACC CAAGGAAGAG TCGGAAGAGG GGAACTCGGC TGAGTCGGCT 
GCAGAGGAGA GAGACCAGGA TAAGAAACGC AGAGTTGTAG ACACAGAGAG TGGTGCTGCA 
GCTGCTGTGG AGAAACTGGA AGAGGTAACA GCGGGAACCC AGCTGGGTCC GGAAGAGCCA 
TGTGAACAGG AAGATGACAA CAGGAGTCTT CGACGTCACA CCAGAGAGCT ATCATTGAGG 
CGGAAATCAA AGGAGGATCC AGACAGAGAA GCAAGACCGG AAACTCACTT GGACGAGGAC 
GAGGACGGAA AAAAGGATAA AAGAAGTTCC AGACCCAGGA GCCAGCCCAG AGATCCAGCT 
GCCAAACGGA GACCCAAGGA AGCAGAGCCA GAGCAGGTAG CTCCAGAGAC TCCCGAGGAC 
AGAGACGAGG ATGAGAGGGA GGAGAAGAGA CGAAAAACGA CACGTAAAAA ACTGGAGTCA 
CACACCGTTC CCGTTCAGAG CAGATCGGAG AGAAAAGCCG CTCAAAGCAA AAGTGTGATC 
CCGAAGATCA ACTCACCAAA GTGCCCCGAG TGTGGCCAGC ACCTAGACGA CCCTAACCTG 
AAGTACCAGC AGCACCCTGA GGATGCTGTG GATGAACCCC AGATGTTGAC CAGTGAGAAA 
CTGTCCATCT ACGACTCCAC CTCGACCTGG TTTGATACTT ATGAAGATTC TCCCATGCAT 
AGGTTCACTT CCTTCAGTGT GTACTGCAGT CGCGGGCACC TGTGTCCTGT CGACACCGGT 
CTCATTGAGA AGAATGTAGA GCTCTACTTT TCTGGGTGTG CCAAAGCAAT TCATGACGAG 
AATCCATCTA TGGAAGGTGG TATTAATGGC AAAAACCTCG GGCCAATCAA TCAGTGGTGG 
CTCAGTGGCT TTGATGGTGG CGAGAAGGTG CTCATTGGCT TCTCCACTGC ATTTGCTGAA 
TACATTTTGA TGGAGCCCAG CAAAGAGTAT GAGCCAATAT TTGGGCTGAT GCAGGAGAAA 
ATTTACATCA GCAAGATTGT TGTTGAGTTC CTGCAAAACA ATCCTGATGC TGTATATGAA 
GACCTGATCA ATAAGATTGA GACCACTGTT CCTCCTTCTA CCATTAATGT GAACCGGTTC 
ACAGAGGACT CCCTCTTACG CCACGCCCAG TTTGTAGTGA GCCAGGTAGA GAGTTACGAC 
GAAGCCAAGG ACGATGATGA GACCCCCATC TTCTTGTCTC CCTGTATGAG AGCCCTGATC 
CATTTGGCTG GTGTCTCCCT GGGACAGAGG CGAGCAACAA GGCGCGTCAT GGGTGCTACC 
AAGGAGAAGG ACAAAGCACC CACGAAAGCC ACCACCACCA AGCTGGTCTA TCAGATCTTT 
GACACTTTCT TCTCAGAGCA GATTGAGAAG TATGATAAGG AGGACAAGGA GAATGCCATG 
AAGCGCCGCC GCTGTGGTGT CTGTGAGGTC TGTCAGCAGC CTGAGTGTGG GAAGTGCAAG 
GCGTGCAAAG ATATGGTGAA GTTTGGTGGC ACTGGACGGA GTAAGCAGGC TTGCCTCAAG 
AGGAGGTGTC CTAACTTGGC GGTGAAGGAG GCAGACGACG ATGAAGAGGC TGATGATGAT 
GTGTCAGAGA TGCCATCACC CAAAAAGCTG CATCAGGGGA AGAAGAAGAA GCAGAACAAG 
GACCGCATCT CCTGGCTTGG GCAGCCTATG AAGATTGAAG AGAATAGAAC TTACTATCAG 
AAGGTGAGCA TCGATGAGGA GATGCTAGAG GTGGGCGACT GCGTCTCGGT CATTCCAGAT 
GATTCCTCCA AACCACTCTA TCTAGCCAGG GTCACAGCTC TGTGGGAAGA CAAAAATGGT 
CAGATGATGT TCCATGCGCA CTGGTTCTGC GCTGGGACAG ACACAGTCCT GGGAGCCACC 
TCCGACCCCC TGGAACTGTT CCTGGTGGGC GAGTGCGAAA ACATGCAGCT TTCCTACATC 
CACAGCAAGG TCAAGGTCAT CTACAAAGCC CCTTCTGAAA ACTGGGCCAT GGAGGGAGGC 
ACAGACCCTG AGACCACACT GCCTGGGGCT GAGGATGGCA AGACTTACTT CTTCCAGCTC 
TGGTACAACC AGGAGTACGC AAGGTTTGAA TCCCCACCCA AGACCCAGCC GACCGAGGAC 
AACAAGCACA AGTTCTGCCT ATCTTGTATC CGGCTGGCTG AGCTGAGACA AAAAGAAATG 
CCCAAGGTCC TGGAACAAAT TGAGGAGGTG GATGGCCGGG TCTACTGCAG TTCCATCACC 
AAGAATGGTG TTGTCTACCG ACTGGGTGAC AGTGTGTACC TTCCTCCCGA GGCCTTTACT 
TTCAACATCA AAGTGGCTAG CCCCGTGAAA CGCCCAAAGA AGGATCCTGT GAACGAGACC 
CTGTACCCTG AGCACTACCG CAAGTATTCT GACTACATCA AGGGGAGCAA CCTGGATGCT 
CCAGAGCCCT ATCGCATCGG TCGGATAAAA GAGATCCACT GTGGCAAGAA GAAAGGCAAG 
GTCAACGAGG CAGACATCAA GCTGAGGCTC TACAAGTTCT ACAGGCCTGA GAATACCCAC 
AGGTCCTACA ACGGATCCTA TCACACTGAC ATCAACATGC TTTACTGGAG CGACGAGGAA 
GCTGTGGTGA ACTTCAGCGA CGTGCAGGGC CGCTGTACCG TGGAGTACGG GGAAGACCTA 
CTTGAGAGCA TCCAGGATTA TTCACAAGGG GGCCCTGACC GCTTCTACTT CCTCGAGGCC 
TACAATTCAA AGACCAAGAA CTTTGAAGAC CCACCAAACC ATGCCCGCAG CCCTGGGAAC 
AAAGGGAAAG GGAAGGGGAA AGGGAAGGGG AAGGGGAAGC ATCAGGTGTC AGAGCCCAAA 
GAGCCTGAGG CAGCCATCAA ACTGCCCAAG CTCCGGACCC TGGATGTGTT TTCCGGCTGT 
GGAGGGTTAT CGGAAGGATT CCACCAAGCA GGCATCTCGG AAACGCTGTG GGCCATCGAG 
ATGTGGGACC CGGCAGCCCA GGCATTTCGG CTGAACAACC CCGGCACCAC AGTGTTCACA 
GAGGACTGCA ACGTGCTTCT TAAGCTGGTC ATGGCTGGGG AGGTGACCAA CTCTCTGGGC 
CAAAGGCTGC CACAGAAGGG CGATGTGGAG ATGCTGTGTG GTGGGCCACC CTGCCAGGGC 
TTCAGTGGCA TGAACCGCTT CAACTCCCGC ACTTACTCCA AGTTCAAAAA CTCCCTAGTG 
GTCTCCTTCC TCAGCTACTG TGACTACTAC CGGCCTCGGT TCTTCCTTCT GGAGAACGTC 
AGGAACTTCG TGTCCTACAG ACGCTCCATG GTGCTGAAGC TCACACTGCG CTGCCTGGTC 
CGCATGGGCT ACCAGTGCAC CTTTGGTGTG CTCCAGGCTG GACAGTATGG CGTGGCCCAG 
ACACGAAGGA GGGCCATCAT CTTGGCTGCA GCCCCAGGAG AAAAGCTGCC TCTGTTCCCA 
GAGCCTCTGC ATGTGTTTGC GCCCCGTGCC TGCCAGCTGA GCGTTGTGGT GGATGACAAG 
AAGTTTGTTA GCAACATAAC GAGGCTGAGC TCGGGGCCCT TCCGAACCAT CACCGTGCGA 
GACACCATGT CTGACCTCCC CGAGATCCAG AATGGAGCCT CGAATTCTGA GATCCCCTAC 
AATGGAGAGC CACTGTCCTG GTTCCAGAGG CAGCTGCGAG GATCACACTA CCAGCCCATC 
CTCAGGGACC ATATCTGCAA GGACATGAGC CCACTGGTGG CTGCCCGCAT GCGGCACATC 
CCACTGTTCC CAGGATCAGA TTGGCGTGAC CTGCCCAACA TACAGGTGCG GCTGGGAGAT 
GGCGTCATAG CCCATAAGCT ACAGTACACC TTTCATGATG TGAAAAATGG CTACAGCAGC 
ACCGGTGCCC TGCGTGGAGT CTGTTCCTGT GCAGAAGGCA AGGCCTGCGA CCCTGAGTCC 
AGGCAATTCA GCACCCTCAT CCCCTGGTGC CTGCCGCACA CTGGGAACCG GCACAACCAC 
TGGGCTGGCC TCTACGGGCG TCTGGAGTGG GATGGCTTCT TCAGCACCAC TGTCACCAAC 
CCTGAGCCCA TGGGCAAGCA GGGTCGGGTG CTCCACCCGG AGCAGCACCG GGTCGTGAGT 
GTTCGGGAAT GTGCCCGCTC CCAGGGCTTT CCAGATAGCT ACCGGTTCTT CGGCAACATC 
CTGGACAGAC ACCGGCAGGT GGGTAATGCT GTGCCACCAC CCCTGGCCAA AGCCATTGGC 
CTGGAGATTA AGCTCTGCCT GCTGTCCAGT GCTCGGGAGA GCGCATCAGC TGCAGTTAAA 
GCAAAAGAGG AGGCTGCTAC CAAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnmt1 ( NM_001199432.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001186361.1 Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 3, mRNA.

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CloneID	OMu02256
Clone ID Related Accession (Same CDS sequence)	NM_001199432.1
Accession Version	NM_001199432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	DNA (cytosine-5)-methyltransferase 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF036007.1, AK136213.1, AF162282.1 and AK147143.1. Transcript Variant: This variant (3) uses an alternate first exon, which results in the use of a downstream start codon, and an alternate in-frame splice site compared to variant 1. The resulting protein (isoform 3) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF036007.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCAGACT CAAATAGATC CCCAAGATCC AGGCCCAAGC CTCGGGGACC CAGGAGAAGC 
AAGTCGGACA GTGACACCCT TTTTGAAACT TCACCTAGTT CCGTGGCTAC GAGGAGAACC 
ACCAGGCAGA CCACCATCAC GGCTCACTTC ACGAAGGGCC CCACTAAACG GAAACCCAAG 
GAAGAGTCGG AAGAGGGGAA CTCGGCTGAG TCGGCTGCAG AGGAGAGAGA CCAGGATAAG 
AAACGCAGAG TTGTAGACAC AGAGAGTGGT GCTGCAGCTG CTGTGGAGAA ACTGGAAGAG 
GTAACAGCGG GAACCCAGCT GGGTCCGGAA GAGCCATGTG AACAGGAAGA TGACAACAGG 
AGTCTTCGAC GTCACACCAG AGAGCTATCA TTGAGGCGGA AATCAAAGGA GGATCCAGAC 
AGAGAAGCAA GACCGGAAAC TCACTTGGAC GAGGACGAGG ACGGAAAAAA GGATAAAAGA 
AGTTCCAGAC CCAGGAGCCA GCCCAGAGAT CCAGCTGCCA AACGGAGACC CAAGGAAGCA 
GAGCCAGAGC AGGTAGCTCC AGAGACTCCC GAGGACAGAG ACGAGGATGA GAGGGAGGAG 
AAGAGACGAA AAACGACACG TAAAAAACTG GAGTCACACA CCGTTCCCGT TCAGAGCAGA 
TCGGAGAGAA AAGCCGCTCA AAGCAAAAGT GTGATCCCGA AGATCAACTC ACCAAAGTGC 
CCCGAGTGTG GCCAGCACCT AGACGACCCT AACCTGAAGT ACCAGCAGCA CCCTGAGGAT 
GCTGTGGATG AACCCCAGAT GTTGACCAGT GAGAAACTGT CCATCTACGA CTCCACCTCG 
ACCTGGTTTG ATACTTATGA AGATTCTCCC ATGCATAGGT TCACTTCCTT CAGTGTGTAC 
TGCAGTCGCG GGCACCTGTG TCCTGTCGAC ACCGGTCTCA TTGAGAAGAA TGTAGAGCTC 
TACTTTTCTG GGTGTGCCAA AGCAATTCAT GACGAGAATC CATCTATGGA AGGTGGTATT 
AATGGCAAAA ACCTCGGGCC AATCAATCAG TGGTGGCTCA GTGGCTTTGA TGGTGGCGAG 
AAGGTGCTCA TTGGCTTCTC CACTGCATTT GCTGAATACA TTTTGATGGA GCCCAGCAAA 
GAGTATGAGC CAATATTTGG GCTGATGCAG GAGAAAATTT ACATCAGCAA GATTGTTGTT 
GAGTTCCTGC AAAACAATCC TGATGCTGTA TATGAAGACC TGATCAATAA GATTGAGACC 
ACTGTTCCTC CTTCTACCAT TAATGTGAAC CGGTTCACAG AGGACTCCCT CTTACGCCAC 
GCCCAGTTTG TAGTGAGCCA GGTAGAGAGT TACGACGAAG CCAAGGACGA TGATGAGACC 
CCCATCTTCT TGTCTCCCTG TATGAGAGCC CTGATCCATT TGGCTGGTGT CTCCCTGGGA 
CAGAGGCGAG CAACAAGGCG CGTCATGGGT GCTACCAAGG AGAAGGACAA AGCACCCACG 
AAAGCCACCA CCACCAAGCT GGTCTATCAG ATCTTTGACA CTTTCTTCTC AGAGCAGATT 
GAGAAGTATG ATAAGGAGGA CAAGGAGAAT GCCATGAAGC GCCGCCGCTG TGGTGTCTGT 
GAGGTCTGTC AGCAGCCTGA GTGTGGGAAG TGCAAGGCGT GCAAAGATAT GGTGAAGTTT 
GGTGGCACTG GACGGAGTAA GCAGGCTTGC CTCAAGAGGA GGTGTCCTAA CTTGGCGGTG 
AAGGAGGCAG ACGACGATGA AGAGGCTGAT GATGATGTGT CAGAGATGCC ATCACCCAAA 
AAGCTGCATC AGGGGAAGAA GAAGAAGCAG AACAAGGACC GCATCTCCTG GCTTGGGCAG 
CCTATGAAGA TTGAAGAGAA TAGAACTTAC TATCAGAAGG TGAGCATCGA TGAGGAGATG 
CTAGAGGTGG GCGACTGCGT CTCGGTCATT CCAGATGATT CCTCCAAACC ACTCTATCTA 
GCCAGGGTCA CAGCTCTGTG GGAAGACAAA AATGGTCAGA TGATGTTCCA TGCGCACTGG 
TTCTGCGCTG GGACAGACAC AGTCCTGGGA GCCACCTCCG ACCCCCTGGA ACTGTTCCTG 
GTGGGCGAGT GCGAAAACAT GCAGCTTTCC TACATCCACA GCAAGGTCAA GGTCATCTAC 
AAAGCCCCTT CTGAAAACTG GGCCATGGAG GGAGGCACAG ACCCTGAGAC CACACTGCCT 
GGGGCTGAGG ATGGCAAGAC TTACTTCTTC CAGCTCTGGT ACAACCAGGA GTACGCAAGG 
TTTGAATCCC CACCCAAGAC CCAGCCGACC GAGGACAACA AGCACAAGTT CTGCCTATCT 
TGTATCCGGC TGGCTGAGCT GAGACAAAAA GAAATGCCCA AGGTCCTGGA ACAAATTGAG 
GAGGTGGATG GCCGGGTCTA CTGCAGTTCC ATCACCAAGA ATGGTGTTGT CTACCGACTG 
GGTGACAGTG TGTACCTTCC TCCCGAGGCC TTTACTTTCA ACATCAAAGT GGCTAGCCCC 
GTGAAACGCC CAAAGAAGGA TCCTGTGAAC GAGACCCTGT ACCCTGAGCA CTACCGCAAG 
TATTCTGACT ACATCAAGGG GAGCAACCTG GATGCTCCAG AGCCCTATCG CATCGGTCGG 
ATAAAAGAGA TCCACTGTGG CAAGAAGAAA GGCAAGGTCA ACGAGGCAGA CATCAAGCTG 
AGGCTCTACA AGTTCTACAG GCCTGAGAAT ACCCACAGGT CCTACAACGG ATCCTATCAC 
ACTGACATCA ACATGCTTTA CTGGAGCGAC GAGGAAGCTG TGGTGAACTT CAGCGACGTG 
CAGGGCCGCT GTACCGTGGA GTACGGGGAA GACCTACTTG AGAGCATCCA GGATTATTCA 
CAAGGGGGCC CTGACCGCTT CTACTTCCTC GAGGCCTACA ATTCAAAGAC CAAGAACTTT 
GAAGACCCAC CAAACCATGC CCGCAGCCCT GGGAACAAAG GGAAAGGGAA GGGGAAAGGG 
AAGGGGAAGG GGAAGCATCA GGTGTCAGAG CCCAAAGAGC CTGAGGCAGC CATCAAACTG 
CCCAAGCTCC GGACCCTGGA TGTGTTTTCC GGCTGTGGAG GGTTATCGGA AGGATTCCAC 
CAAGCAGGCA TCTCGGAAAC GCTGTGGGCC ATCGAGATGT GGGACCCGGC AGCCCAGGCA 
TTTCGGCTGA ACAACCCCGG CACCACAGTG TTCACAGAGG ACTGCAACGT GCTTCTTAAG 
CTGGTCATGG CTGGGGAGGT GACCAACTCT CTGGGCCAAA GGCTGCCACA GAAGGGCGAT 
GTGGAGATGC TGTGTGGTGG GCCACCCTGC CAGGGCTTCA GTGGCATGAA CCGCTTCAAC 
TCCCGCACTT ACTCCAAGTT CAAAAACTCC CTAGTGGTCT CCTTCCTCAG CTACTGTGAC 
TACTACCGGC CTCGGTTCTT CCTTCTGGAG AACGTCAGGA ACTTCGTGTC CTACAGACGC 
TCCATGGTGC TGAAGCTCAC ACTGCGCTGC CTGGTCCGCA TGGGCTACCA GTGCACCTTT 
GGTGTGCTCC AGGCTGGACA GTATGGCGTG GCCCAGACAC GAAGGAGGGC CATCATCTTG 
GCTGCAGCCC CAGGAGAAAA GCTGCCTCTG TTCCCAGAGC CTCTGCATGT GTTTGCGCCC 
CGTGCCTGCC AGCTGAGCGT TGTGGTGGAT GACAAGAAGT TTGTTAGCAA CATAACGAGG 
CTGAGCTCGG GGCCCTTCCG AACCATCACC GTGCGAGACA CCATGTCTGA CCTCCCCGAG 
ATCCAGAATG GAGCCTCGAA TTCTGAGATC CCCTACAATG GAGAGCCACT GTCCTGGTTC 
CAGAGGCAGC TGCGAGGATC ACACTACCAG CCCATCCTCA GGGACCATAT CTGCAAGGAC 
ATGAGCCCAC TGGTGGCTGC CCGCATGCGG CACATCCCAC TGTTCCCAGG ATCAGATTGG 
CGTGACCTGC CCAACATACA GGTGCGGCTG GGAGATGGCG TCATAGCCCA TAAGCTACAG 
TACACCTTTC ATGATGTGAA AAATGGCTAC AGCAGCACCG GTGCCCTGCG TGGAGTCTGT 
TCCTGTGCAG AAGGCAAGGC CTGCGACCCT GAGTCCAGGC AATTCAGCAC CCTCATCCCC 
TGGTGCCTGC CGCACACTGG GAACCGGCAC AACCACTGGG CTGGCCTCTA CGGGCGTCTG 
GAGTGGGATG GCTTCTTCAG CACCACTGTC ACCAACCCTG AGCCCATGGG CAAGCAGGGT 
CGGGTGCTCC ACCCGGAGCA GCACCGGGTC GTGAGTGTTC GGGAATGTGC CCGCTCCCAG 
GGCTTTCCAG ATAGCTACCG GTTCTTCGGC AACATCCTGG ACAGACACCG GCAGGTGGGT 
AATGCTGTGC CACCACCCCT GGCCAAAGCC ATTGGCCTGG AGATTAAGCT CTGCCTGCTG 
TCCAGTGCTC GGGAGAGCGC ATCAGCTGCA GTTAAAGCAA AAGAGGAGGC TGCTACCAAG 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186361.1
CDS	431..4936
Translation	MADSNRSPRSRPKPRGPRRSKSDSDTLFETSPSSVATRRTTRQTTITAHFTKGPTKRKPKEESEEGNSAESAAEERDQDKKRRVVDTESGAAAAVEKLEEVTAGTQLGPEEPCEQEDDNRSLRRHTRELSLRRKSKEDPDREARPETHLDEDEDGKKDKRSSRPRSQPRDPAAKRRPKEAEPEQVAPETPEDRDEDEREEKRRKTTRKKLESHTVPVQSRSERKAAQSKSVIPKINSPKCPECGQHLDDPNLKYQQHPEDAVDEPQMLTSEKLSIYDSTSTWFDTYEDSPMHRFTSFSVYCSRGHLCPVDTGLIEKNVELYFSGCAKAIHDENPSMEGGINGKNLGPINQWWLSGFDGGEKVLIGFSTAFAEYILMEPSKEYEPIFGLMQEKIYISKIVVEFLQNNPDAVYEDLINKIETTVPPSTINVNRFTEDSLLRHAQFVVSQVESYDEAKDDDETPIFLSPCMRALIHLAGVSLGQRRATRRVMGATKEKDKAPTKATTTKLVYQIFDTFFSEQIEKYDKEDKENAMKRRRCGVCEVCQQPECGKCKACKDMVKFGGTGRSKQACLKRRCPNLAVKEADDDEEADDDVSEMPSPKKLHQGKKKKQNKDRISWLGQPMKIEENRTYYQKVSIDEEMLEVGDCVSVIPDDSSKPLYLARVTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYIHSKVKVIYKAPSENWAMEGGTDPETTLPGAEDGKTYFFQLWYNQEYARFESPPKTQPTEDNKHKFCLSCIRLAELRQKEMPKVLEQIEEVDGRVYCSSITKNGVVYRLGDSVYLPPEAFTFNIKVASPVKRPKKDPVNETLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPENTHRSYNGSYHTDINMLYWSDEEAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSKTKNFEDPPNHARSPGNKGKGKGKGKGKGKHQVSEPKEPEAAIKLPKLRTLDVFSGCGGLSEGFHQAGISETLWAIEMWDPAAQAFRLNNPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEIQNGASNSEIPYNGEPLSWFQRQLRGSHYQPILRDHICKDMSPLVAARMRHIPLFPGSDWRDLPNIQVRLGDGVIAHKLQYTFHDVKNGYSSTGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDSYRFFGNILDRHRQVGNAVPPPLAKAIGLEIKLCLLSSARESASAAVKAKEEAATKD

Target ORF information:

RefSeq Version	NM_001199432.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199432.1

ORF Insert Sequence:

ATGGCAGACT CAAATAGATC CCCAAGATCC AGGCCCAAGC CTCGGGGACC CAGGAGAAGC 
AAGTCGGACA GTGACACCCT TTTTGAAACT TCACCTAGTT CCGTGGCTAC GAGGAGAACC 
ACCAGGCAGA CCACCATCAC GGCTCACTTC ACGAAGGGCC CCACTAAACG GAAACCCAAG 
GAAGAGTCGG AAGAGGGGAA CTCGGCTGAG TCGGCTGCAG AGGAGAGAGA CCAGGATAAG 
AAACGCAGAG TTGTAGACAC AGAGAGTGGT GCTGCAGCTG CTGTGGAGAA ACTGGAAGAG 
GTAACAGCGG GAACCCAGCT GGGTCCGGAA GAGCCATGTG AACAGGAAGA TGACAACAGG 
AGTCTTCGAC GTCACACCAG AGAGCTATCA TTGAGGCGGA AATCAAAGGA GGATCCAGAC 
AGAGAAGCAA GACCGGAAAC TCACTTGGAC GAGGACGAGG ACGGAAAAAA GGATAAAAGA 
AGTTCCAGAC CCAGGAGCCA GCCCAGAGAT CCAGCTGCCA AACGGAGACC CAAGGAAGCA 
GAGCCAGAGC AGGTAGCTCC AGAGACTCCC GAGGACAGAG ACGAGGATGA GAGGGAGGAG 
AAGAGACGAA AAACGACACG TAAAAAACTG GAGTCACACA CCGTTCCCGT TCAGAGCAGA 
TCGGAGAGAA AAGCCGCTCA AAGCAAAAGT GTGATCCCGA AGATCAACTC ACCAAAGTGC 
CCCGAGTGTG GCCAGCACCT AGACGACCCT AACCTGAAGT ACCAGCAGCA CCCTGAGGAT 
GCTGTGGATG AACCCCAGAT GTTGACCAGT GAGAAACTGT CCATCTACGA CTCCACCTCG 
ACCTGGTTTG ATACTTATGA AGATTCTCCC ATGCATAGGT TCACTTCCTT CAGTGTGTAC 
TGCAGTCGCG GGCACCTGTG TCCTGTCGAC ACCGGTCTCA TTGAGAAGAA TGTAGAGCTC 
TACTTTTCTG GGTGTGCCAA AGCAATTCAT GACGAGAATC CATCTATGGA AGGTGGTATT 
AATGGCAAAA ACCTCGGGCC AATCAATCAG TGGTGGCTCA GTGGCTTTGA TGGTGGCGAG 
AAGGTGCTCA TTGGCTTCTC CACTGCATTT GCTGAATACA TTTTGATGGA GCCCAGCAAA 
GAGTATGAGC CAATATTTGG GCTGATGCAG GAGAAAATTT ACATCAGCAA GATTGTTGTT 
GAGTTCCTGC AAAACAATCC TGATGCTGTA TATGAAGACC TGATCAATAA GATTGAGACC 
ACTGTTCCTC CTTCTACCAT TAATGTGAAC CGGTTCACAG AGGACTCCCT CTTACGCCAC 
GCCCAGTTTG TAGTGAGCCA GGTAGAGAGT TACGACGAAG CCAAGGACGA TGATGAGACC 
CCCATCTTCT TGTCTCCCTG TATGAGAGCC CTGATCCATT TGGCTGGTGT CTCCCTGGGA 
CAGAGGCGAG CAACAAGGCG CGTCATGGGT GCTACCAAGG AGAAGGACAA AGCACCCACG 
AAAGCCACCA CCACCAAGCT GGTCTATCAG ATCTTTGACA CTTTCTTCTC AGAGCAGATT 
GAGAAGTATG ATAAGGAGGA CAAGGAGAAT GCCATGAAGC GCCGCCGCTG TGGTGTCTGT 
GAGGTCTGTC AGCAGCCTGA GTGTGGGAAG TGCAAGGCGT GCAAAGATAT GGTGAAGTTT 
GGTGGCACTG GACGGAGTAA GCAGGCTTGC CTCAAGAGGA GGTGTCCTAA CTTGGCGGTG 
AAGGAGGCAG ACGACGATGA AGAGGCTGAT GATGATGTGT CAGAGATGCC ATCACCCAAA 
AAGCTGCATC AGGGGAAGAA GAAGAAGCAG AACAAGGACC GCATCTCCTG GCTTGGGCAG 
CCTATGAAGA TTGAAGAGAA TAGAACTTAC TATCAGAAGG TGAGCATCGA TGAGGAGATG 
CTAGAGGTGG GCGACTGCGT CTCGGTCATT CCAGATGATT CCTCCAAACC ACTCTATCTA 
GCCAGGGTCA CAGCTCTGTG GGAAGACAAA AATGGTCAGA TGATGTTCCA TGCGCACTGG 
TTCTGCGCTG GGACAGACAC AGTCCTGGGA GCCACCTCCG ACCCCCTGGA ACTGTTCCTG 
GTGGGCGAGT GCGAAAACAT GCAGCTTTCC TACATCCACA GCAAGGTCAA GGTCATCTAC 
AAAGCCCCTT CTGAAAACTG GGCCATGGAG GGAGGCACAG ACCCTGAGAC CACACTGCCT 
GGGGCTGAGG ATGGCAAGAC TTACTTCTTC CAGCTCTGGT ACAACCAGGA GTACGCAAGG 
TTTGAATCCC CACCCAAGAC CCAGCCGACC GAGGACAACA AGCACAAGTT CTGCCTATCT 
TGTATCCGGC TGGCTGAGCT GAGACAAAAA GAAATGCCCA AGGTCCTGGA ACAAATTGAG 
GAGGTGGATG GCCGGGTCTA CTGCAGTTCC ATCACCAAGA ATGGTGTTGT CTACCGACTG 
GGTGACAGTG TGTACCTTCC TCCCGAGGCC TTTACTTTCA ACATCAAAGT GGCTAGCCCC 
GTGAAACGCC CAAAGAAGGA TCCTGTGAAC GAGACCCTGT ACCCTGAGCA CTACCGCAAG 
TATTCTGACT ACATCAAGGG GAGCAACCTG GATGCTCCAG AGCCCTATCG CATCGGTCGG 
ATAAAAGAGA TCCACTGTGG CAAGAAGAAA GGCAAGGTCA ACGAGGCAGA CATCAAGCTG 
AGGCTCTACA AGTTCTACAG GCCTGAGAAT ACCCACAGGT CCTACAACGG ATCCTATCAC 
ACTGACATCA ACATGCTTTA CTGGAGCGAC GAGGAAGCTG TGGTGAACTT CAGCGACGTG 
CAGGGCCGCT GTACCGTGGA GTACGGGGAA GACCTACTTG AGAGCATCCA GGATTATTCA 
CAAGGGGGCC CTGACCGCTT CTACTTCCTC GAGGCCTACA ATTCAAAGAC CAAGAACTTT 
GAAGACCCAC CAAACCATGC CCGCAGCCCT GGGAACAAAG GGAAAGGGAA GGGGAAAGGG 
AAGGGGAAGG GGAAGCATCA GGTGTCAGAG CCCAAAGAGC CTGAGGCAGC CATCAAACTG 
CCCAAGCTCC GGACCCTGGA TGTGTTTTCC GGCTGTGGAG GGTTATCGGA AGGATTCCAC 
CAAGCAGGCA TCTCGGAAAC GCTGTGGGCC ATCGAGATGT GGGACCCGGC AGCCCAGGCA 
TTTCGGCTGA ACAACCCCGG CACCACAGTG TTCACAGAGG ACTGCAACGT GCTTCTTAAG 
CTGGTCATGG CTGGGGAGGT GACCAACTCT CTGGGCCAAA GGCTGCCACA GAAGGGCGAT 
GTGGAGATGC TGTGTGGTGG GCCACCCTGC CAGGGCTTCA GTGGCATGAA CCGCTTCAAC 
TCCCGCACTT ACTCCAAGTT CAAAAACTCC CTAGTGGTCT CCTTCCTCAG CTACTGTGAC 
TACTACCGGC CTCGGTTCTT CCTTCTGGAG AACGTCAGGA ACTTCGTGTC CTACAGACGC 
TCCATGGTGC TGAAGCTCAC ACTGCGCTGC CTGGTCCGCA TGGGCTACCA GTGCACCTTT 
GGTGTGCTCC AGGCTGGACA GTATGGCGTG GCCCAGACAC GAAGGAGGGC CATCATCTTG 
GCTGCAGCCC CAGGAGAAAA GCTGCCTCTG TTCCCAGAGC CTCTGCATGT GTTTGCGCCC 
CGTGCCTGCC AGCTGAGCGT TGTGGTGGAT GACAAGAAGT TTGTTAGCAA CATAACGAGG 
CTGAGCTCGG GGCCCTTCCG AACCATCACC GTGCGAGACA CCATGTCTGA CCTCCCCGAG 
ATCCAGAATG GAGCCTCGAA TTCTGAGATC CCCTACAATG GAGAGCCACT GTCCTGGTTC 
CAGAGGCAGC TGCGAGGATC ACACTACCAG CCCATCCTCA GGGACCATAT CTGCAAGGAC 
ATGAGCCCAC TGGTGGCTGC CCGCATGCGG CACATCCCAC TGTTCCCAGG ATCAGATTGG 
CGTGACCTGC CCAACATACA GGTGCGGCTG GGAGATGGCG TCATAGCCCA TAAGCTACAG 
TACACCTTTC ATGATGTGAA AAATGGCTAC AGCAGCACCG GTGCCCTGCG TGGAGTCTGT 
TCCTGTGCAG AAGGCAAGGC CTGCGACCCT GAGTCCAGGC AATTCAGCAC CCTCATCCCC 
TGGTGCCTGC CGCACACTGG GAACCGGCAC AACCACTGGG CTGGCCTCTA CGGGCGTCTG 
GAGTGGGATG GCTTCTTCAG CACCACTGTC ACCAACCCTG AGCCCATGGG CAAGCAGGGT 
CGGGTGCTCC ACCCGGAGCA GCACCGGGTC GTGAGTGTTC GGGAATGTGC CCGCTCCCAG 
GGCTTTCCAG ATAGCTACCG GTTCTTCGGC AACATCCTGG ACAGACACCG GCAGGTGGGT 
AATGCTGTGC CACCACCCCT GGCCAAAGCC ATTGGCCTGG AGATTAAGCT CTGCCTGCTG 
TCCAGTGCTC GGGAGAGCGC ATCAGCTGCA GTTAAAGCAA AAGAGGAGGC TGCTACCAAG 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnmt1 ( NM_001199431.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001186360.2 Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 1, mRNA.

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CloneID	OMu15704
Clone ID Related Accession (Same CDS sequence)	NM_001199431.1
Accession Version	NM_001199431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	DNA (cytosine-5)-methyltransferase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF162282.1 and AK147143.1. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). This variant has two upstream in-frame AUGs but N-terminal sequencing and western analysis from PMID: 9830015 (Table I and Figure 2) support the use of the third in-frame AUG. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF162282.1, BC048148.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9830015) ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCAGCGC GAACAGCTCC AGCCCGAGTG CCTGCGCTTG CCTCCCCGGC AGGCTCGCTC 
CCGGACCATG TCCGCAGGCG GCTCAAAGAC TTGGAAAGAG ATGGCTTAAC AGAAAAGGAG 
TGTGTGAGGG AGAAATTAAA CTTACTGCAT GAATTCCTGC AAACAGAAAT AAAAAGCCAG 
TTGTGTGACT TGGAAACCAA ATTACATAAA GAGGAATTAT CTGAGGAAGG CTACCTGGCT 
AAAGTCAAGT CCCTCTTAAA TAAGGATTTG TCCTTGGAGA ACGGAACACA CACTCTCACT 
CAAAAAGCCA ACGGTTGTCC CGCCAACGGG AGCCGGCCAA CCTGGAGAGC AGAAATGGCA 
GACTCAAATA GATCCCCAAG ATCCAGGCCC AAGCCTCGGG GACCCAGGAG AAGCAAGTCG 
GACAGTGACA CCCTTTCAGT TGAAACTTCA CCTAGTTCCG TGGCTACGAG GAGAACCACC 
AGGCAGACCA CCATCACGGC TCACTTCACG AAGGGCCCCA CTAAACGGAA ACCCAAGGAA 
GAGTCGGAAG AGGGGAACTC GGCTGAGTCG GCTGCAGAGG AGAGAGACCA GGATAAGAAA 
CGCAGAGTTG TAGACACAGA GAGTGGTGCT GCAGCTGCTG TGGAGAAACT GGAAGAGGTA 
ACAGCGGGAA CCCAGCTGGG TCCGGAAGAG CCATGTGAAC AGGAAGATGA CAACAGGAGT 
CTTCGACGTC ACACCAGAGA GCTATCATTG AGGCGGAAAT CAAAGGAGGA TCCAGACAGA 
GAAGCAAGAC CGGAAACTCA CTTGGACGAG GACGAGGACG GAAAAAAGGA TAAAAGAAGT 
TCCAGACCCA GGAGCCAGCC CAGAGATCCA GCTGCCAAAC GGAGACCCAA GGAAGCAGAG 
CCAGAGCAGG TAGCTCCAGA GACTCCCGAG GACAGAGACG AGGATGAGAG GGAGGAGAAG 
AGACGAAAAA CGACACGTAA AAAACTGGAG TCACACACCG TTCCCGTTCA GAGCAGATCG 
GAGAGAAAAG CCGCTCAAAG CAAAAGTGTG ATCCCGAAGA TCAACTCACC AAAGTGCCCC 
GAGTGTGGCC AGCACCTAGA CGACCCTAAC CTGAAGTACC AGCAGCACCC TGAGGATGCT 
GTGGATGAAC CCCAGATGTT GACCAGTGAG AAACTGTCCA TCTACGACTC CACCTCGACC 
TGGTTTGATA CTTATGAAGA TTCTCCCATG CATAGGTTCA CTTCCTTCAG TGTGTACTGC 
AGTCGCGGGC ACCTGTGTCC TGTCGACACC GGTCTCATTG AGAAGAATGT AGAGCTCTAC 
TTTTCTGGGT GTGCCAAAGC AATTCATGAC GAGAATCCAT CTATGGAAGG TGGTATTAAT 
GGCAAAAACC TCGGGCCAAT CAATCAGTGG TGGCTCAGTG GCTTTGATGG TGGCGAGAAG 
GTGCTCATTG GCTTCTCCAC TGCATTTGCT GAATACATTT TGATGGAGCC CAGCAAAGAG 
TATGAGCCAA TATTTGGGCT GATGCAGGAG AAAATTTACA TCAGCAAGAT TGTTGTTGAG 
TTCCTGCAAA ACAATCCTGA TGCTGTATAT GAAGACCTGA TCAATAAGAT TGAGACCACT 
GTTCCTCCTT CTACCATTAA TGTGAACCGG TTCACAGAGG ACTCCCTCTT ACGCCACGCC 
CAGTTTGTAG TGAGCCAGGT AGAGAGTTAC GACGAAGCCA AGGACGATGA TGAGACCCCC 
ATCTTCTTGT CTCCCTGTAT GAGAGCCCTG ATCCATTTGG CTGGTGTCTC CCTGGGACAG 
AGGCGAGCAA CAAGGCGCGT CATGGGTGCT ACCAAGGAGA AGGACAAAGC ACCCACGAAA 
GCCACCACCA CCAAGCTGGT CTATCAGATC TTTGACACTT TCTTCTCAGA GCAGATTGAG 
AAGTATGATA AGGAGGACAA GGAGAATGCC ATGAAGCGCC GCCGCTGTGG TGTCTGTGAG 
GTCTGTCAGC AGCCTGAGTG TGGGAAGTGC AAGGCGTGCA AAGATATGGT GAAGTTTGGT 
GGCACTGGAC GGAGTAAGCA GGCTTGCCTC AAGAGGAGGT GTCCTAACTT GGCGGTGAAG 
GAGGCAGACG ACGATGAAGA GGCTGATGAT GATGTGTCAG AGATGCCATC ACCCAAAAAG 
CTGCATCAGG GGAAGAAGAA GAAGCAGAAC AAGGACCGCA TCTCCTGGCT TGGGCAGCCT 
ATGAAGATTG AAGAGAATAG AACTTACTAT CAGAAGGTGA GCATCGATGA GGAGATGCTA 
GAGGTGGGCG ACTGCGTCTC GGTCATTCCA GATGATTCCT CCAAACCACT CTATCTAGCC 
AGGGTCACAG CTCTGTGGGA AGACAAAAAT GGTCAGATGA TGTTCCATGC GCACTGGTTC 
TGCGCTGGGA CAGACACAGT CCTGGGAGCC ACCTCCGACC CCCTGGAACT GTTCCTGGTG 
GGCGAGTGCG AAAACATGCA GCTTTCCTAC ATCCACAGCA AGGTCAAGGT CATCTACAAA 
GCCCCTTCTG AAAACTGGGC CATGGAGGGA GGCACAGACC CTGAGACCAC ACTGCCTGGG 
GCTGAGGATG GCAAGACTTA CTTCTTCCAG CTCTGGTACA ACCAGGAGTA CGCAAGGTTT 
GAATCCCCAC CCAAGACCCA GCCGACCGAG GACAACAAGC ACAAGTTCTG CCTATCTTGT 
ATCCGGCTGG CTGAGCTGAG ACAAAAAGAA ATGCCCAAGG TCCTGGAACA AATTGAGGAG 
GTGGATGGCC GGGTCTACTG CAGTTCCATC ACCAAGAATG GTGTTGTCTA CCGACTGGGT 
GACAGTGTGT ACCTTCCTCC CGAGGCCTTT ACTTTCAACA TCAAAGTGGC TAGCCCCGTG 
AAACGCCCAA AGAAGGATCC TGTGAACGAG ACCCTGTACC CTGAGCACTA CCGCAAGTAT 
TCTGACTACA TCAAGGGGAG CAACCTGGAT GCTCCAGAGC CCTATCGCAT CGGTCGGATA 
AAAGAGATCC ACTGTGGCAA GAAGAAAGGC AAGGTCAACG AGGCAGACAT CAAGCTGAGG 
CTCTACAAGT TCTACAGGCC TGAGAATACC CACAGGTCCT ACAACGGATC CTATCACACT 
GACATCAACA TGCTTTACTG GAGCGACGAG GAAGCTGTGG TGAACTTCAG CGACGTGCAG 
GGCCGCTGTA CCGTGGAGTA CGGGGAAGAC CTACTTGAGA GCATCCAGGA TTATTCACAA 
GGGGGCCCTG ACCGCTTCTA CTTCCTCGAG GCCTACAATT CAAAGACCAA GAACTTTGAA 
GACCCACCAA ACCATGCCCG CAGCCCTGGG AACAAAGGGA AAGGGAAGGG GAAAGGGAAG 
GGGAAGGGGA AGCATCAGGT GTCAGAGCCC AAAGAGCCTG AGGCAGCCAT CAAACTGCCC 
AAGCTCCGGA CCCTGGATGT GTTTTCCGGC TGTGGAGGGT TATCGGAAGG ATTCCACCAA 
GCAGGCATCT CGGAAACGCT GTGGGCCATC GAGATGTGGG ACCCGGCAGC CCAGGCATTT 
CGGCTGAACA ACCCCGGCAC CACAGTGTTC ACAGAGGACT GCAACGTGCT TCTTAAGCTG 
GTCATGGCTG GGGAGGTGAC CAACTCTCTG GGCCAAAGGC TGCCACAGAA GGGCGATGTG 
GAGATGCTGT GTGGTGGGCC ACCCTGCCAG GGCTTCAGTG GCATGAACCG CTTCAACTCC 
CGCACTTACT CCAAGTTCAA AAACTCCCTA GTGGTCTCCT TCCTCAGCTA CTGTGACTAC 
TACCGGCCTC GGTTCTTCCT TCTGGAGAAC GTCAGGAACT TCGTGTCCTA CAGACGCTCC 
ATGGTGCTGA AGCTCACACT GCGCTGCCTG GTCCGCATGG GCTACCAGTG CACCTTTGGT 
GTGCTCCAGG CTGGACAGTA TGGCGTGGCC CAGACACGAA GGAGGGCCAT CATCTTGGCT 
GCAGCCCCAG GAGAAAAGCT GCCTCTGTTC CCAGAGCCTC TGCATGTGTT TGCGCCCCGT 
GCCTGCCAGC TGAGCGTTGT GGTGGATGAC AAGAAGTTTG TTAGCAACAT AACGAGGCTG 
AGCTCGGGGC CCTTCCGAAC CATCACCGTG CGAGACACCA TGTCTGACCT CCCCGAGATC 
CAGAATGGAG CCTCGAATTC TGAGATCCCC TACAATGGAG AGCCACTGTC CTGGTTCCAG 
AGGCAGCTGC GAGGATCACA CTACCAGCCC ATCCTCAGGG ACCATATCTG CAAGGACATG 
AGCCCACTGG TGGCTGCCCG CATGCGGCAC ATCCCACTGT TCCCAGGATC AGATTGGCGT 
GACCTGCCCA ACATACAGGT GCGGCTGGGA GATGGCGTCA TAGCCCATAA GCTACAGTAC 
ACCTTTCATG ATGTGAAAAA TGGCTACAGC AGCACCGGTG CCCTGCGTGG AGTCTGTTCC 
TGTGCAGAAG GCAAGGCCTG CGACCCTGAG TCCAGGCAAT TCAGCACCCT CATCCCCTGG 
TGCCTGCCGC ACACTGGGAA CCGGCACAAC CACTGGGCTG GCCTCTACGG GCGTCTGGAG 
TGGGATGGCT TCTTCAGCAC CACTGTCACC AACCCTGAGC CCATGGGCAA GCAGGGTCGG 
GTGCTCCACC CGGAGCAGCA CCGGGTCGTG AGTGTTCGGG AATGTGCCCG CTCCCAGGGC 
TTTCCAGATA GCTACCGGTT CTTCGGCAAC ATCCTGGACA GACACCGGCA GGTGGGTAAT 
GCTGTGCCAC CACCCCTGGC CAAAGCCATT GGCCTGGAGA TTAAGCTCTG CCTGCTGTCC 
AGTGCTCGGG AGAGCGCATC AGCTGCAGTT AAAGCAAAAG AGGAGGCTGC TACCAAGGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186360.2
CDS	193..5055
Translation	MPARTAPARVPALASPAGSLPDHVRRRLKDLERDGLTEKECVREKLNLLHEFLQTEIKSQLCDLETKLHKEELSEEGYLAKVKSLLNKDLSLENGTHTLTQKANGCPANGSRPTWRAEMADSNRSPRSRPKPRGPRRSKSDSDTLSVETSPSSVATRRTTRQTTITAHFTKGPTKRKPKEESEEGNSAESAAEERDQDKKRRVVDTESGAAAAVEKLEEVTAGTQLGPEEPCEQEDDNRSLRRHTRELSLRRKSKEDPDREARPETHLDEDEDGKKDKRSSRPRSQPRDPAAKRRPKEAEPEQVAPETPEDRDEDEREEKRRKTTRKKLESHTVPVQSRSERKAAQSKSVIPKINSPKCPECGQHLDDPNLKYQQHPEDAVDEPQMLTSEKLSIYDSTSTWFDTYEDSPMHRFTSFSVYCSRGHLCPVDTGLIEKNVELYFSGCAKAIHDENPSMEGGINGKNLGPINQWWLSGFDGGEKVLIGFSTAFAEYILMEPSKEYEPIFGLMQEKIYISKIVVEFLQNNPDAVYEDLINKIETTVPPSTINVNRFTEDSLLRHAQFVVSQVESYDEAKDDDETPIFLSPCMRALIHLAGVSLGQRRATRRVMGATKEKDKAPTKATTTKLVYQIFDTFFSEQIEKYDKEDKENAMKRRRCGVCEVCQQPECGKCKACKDMVKFGGTGRSKQACLKRRCPNLAVKEADDDEEADDDVSEMPSPKKLHQGKKKKQNKDRISWLGQPMKIEENRTYYQKVSIDEEMLEVGDCVSVIPDDSSKPLYLARVTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYIHSKVKVIYKAPSENWAMEGGTDPETTLPGAEDGKTYFFQLWYNQEYARFESPPKTQPTEDNKHKFCLSCIRLAELRQKEMPKVLEQIEEVDGRVYCSSITKNGVVYRLGDSVYLPPEAFTFNIKVASPVKRPKKDPVNETLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPENTHRSYNGSYHTDINMLYWSDEEAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSKTKNFEDPPNHARSPGNKGKGKGKGKGKGKHQVSEPKEPEAAIKLPKLRTLDVFSGCGGLSEGFHQAGISETLWAIEMWDPAAQAFRLNNPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEIQNGASNSEIPYNGEPLSWFQRQLRGSHYQPILRDHICKDMSPLVAARMRHIPLFPGSDWRDLPNIQVRLGDGVIAHKLQYTFHDVKNGYSSTGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDSYRFFGNILDRHRQVGNAVPPPLAKAIGLEIKLCLLSSARESASAAVKAKEEAATKD

Target ORF information:

RefSeq Version	NM_001199431.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199431.1

ORF Insert Sequence:

ATGCCAGCGC GAACAGCTCC AGCCCGAGTG CCTGCGCTTG CCTCCCCGGC AGGCTCGCTC 
CCGGACCATG TCCGCAGGCG GCTCAAAGAC TTGGAAAGAG ATGGCTTAAC AGAAAAGGAG 
TGTGTGAGGG AGAAATTAAA CTTACTGCAT GAATTCCTGC AAACAGAAAT AAAAAGCCAG 
TTGTGTGACT TGGAAACCAA ATTACATAAA GAGGAATTAT CTGAGGAAGG CTACCTGGCT 
AAAGTCAAGT CCCTCTTAAA TAAGGATTTG TCCTTGGAGA ACGGAACACA CACTCTCACT 
CAAAAAGCCA ACGGTTGTCC CGCCAACGGG AGCCGGCCAA CCTGGAGAGC AGAAATGGCA 
GACTCAAATA GATCCCCAAG ATCCAGGCCC AAGCCTCGGG GACCCAGGAG AAGCAAGTCG 
GACAGTGACA CCCTTTCAGT TGAAACTTCA CCTAGTTCCG TGGCTACGAG GAGAACCACC 
AGGCAGACCA CCATCACGGC TCACTTCACG AAGGGCCCCA CTAAACGGAA ACCCAAGGAA 
GAGTCGGAAG AGGGGAACTC GGCTGAGTCG GCTGCAGAGG AGAGAGACCA GGATAAGAAA 
CGCAGAGTTG TAGACACAGA GAGTGGTGCT GCAGCTGCTG TGGAGAAACT GGAAGAGGTA 
ACAGCGGGAA CCCAGCTGGG TCCGGAAGAG CCATGTGAAC AGGAAGATGA CAACAGGAGT 
CTTCGACGTC ACACCAGAGA GCTATCATTG AGGCGGAAAT CAAAGGAGGA TCCAGACAGA 
GAAGCAAGAC CGGAAACTCA CTTGGACGAG GACGAGGACG GAAAAAAGGA TAAAAGAAGT 
TCCAGACCCA GGAGCCAGCC CAGAGATCCA GCTGCCAAAC GGAGACCCAA GGAAGCAGAG 
CCAGAGCAGG TAGCTCCAGA GACTCCCGAG GACAGAGACG AGGATGAGAG GGAGGAGAAG 
AGACGAAAAA CGACACGTAA AAAACTGGAG TCACACACCG TTCCCGTTCA GAGCAGATCG 
GAGAGAAAAG CCGCTCAAAG CAAAAGTGTG ATCCCGAAGA TCAACTCACC AAAGTGCCCC 
GAGTGTGGCC AGCACCTAGA CGACCCTAAC CTGAAGTACC AGCAGCACCC TGAGGATGCT 
GTGGATGAAC CCCAGATGTT GACCAGTGAG AAACTGTCCA TCTACGACTC CACCTCGACC 
TGGTTTGATA CTTATGAAGA TTCTCCCATG CATAGGTTCA CTTCCTTCAG TGTGTACTGC 
AGTCGCGGGC ACCTGTGTCC TGTCGACACC GGTCTCATTG AGAAGAATGT AGAGCTCTAC 
TTTTCTGGGT GTGCCAAAGC AATTCATGAC GAGAATCCAT CTATGGAAGG TGGTATTAAT 
GGCAAAAACC TCGGGCCAAT CAATCAGTGG TGGCTCAGTG GCTTTGATGG TGGCGAGAAG 
GTGCTCATTG GCTTCTCCAC TGCATTTGCT GAATACATTT TGATGGAGCC CAGCAAAGAG 
TATGAGCCAA TATTTGGGCT GATGCAGGAG AAAATTTACA TCAGCAAGAT TGTTGTTGAG 
TTCCTGCAAA ACAATCCTGA TGCTGTATAT GAAGACCTGA TCAATAAGAT TGAGACCACT 
GTTCCTCCTT CTACCATTAA TGTGAACCGG TTCACAGAGG ACTCCCTCTT ACGCCACGCC 
CAGTTTGTAG TGAGCCAGGT AGAGAGTTAC GACGAAGCCA AGGACGATGA TGAGACCCCC 
ATCTTCTTGT CTCCCTGTAT GAGAGCCCTG ATCCATTTGG CTGGTGTCTC CCTGGGACAG 
AGGCGAGCAA CAAGGCGCGT CATGGGTGCT ACCAAGGAGA AGGACAAAGC ACCCACGAAA 
GCCACCACCA CCAAGCTGGT CTATCAGATC TTTGACACTT TCTTCTCAGA GCAGATTGAG 
AAGTATGATA AGGAGGACAA GGAGAATGCC ATGAAGCGCC GCCGCTGTGG TGTCTGTGAG 
GTCTGTCAGC AGCCTGAGTG TGGGAAGTGC AAGGCGTGCA AAGATATGGT GAAGTTTGGT 
GGCACTGGAC GGAGTAAGCA GGCTTGCCTC AAGAGGAGGT GTCCTAACTT GGCGGTGAAG 
GAGGCAGACG ACGATGAAGA GGCTGATGAT GATGTGTCAG AGATGCCATC ACCCAAAAAG 
CTGCATCAGG GGAAGAAGAA GAAGCAGAAC AAGGACCGCA TCTCCTGGCT TGGGCAGCCT 
ATGAAGATTG AAGAGAATAG AACTTACTAT CAGAAGGTGA GCATCGATGA GGAGATGCTA 
GAGGTGGGCG ACTGCGTCTC GGTCATTCCA GATGATTCCT CCAAACCACT CTATCTAGCC 
AGGGTCACAG CTCTGTGGGA AGACAAAAAT GGTCAGATGA TGTTCCATGC GCACTGGTTC 
TGCGCTGGGA CAGACACAGT CCTGGGAGCC ACCTCCGACC CCCTGGAACT GTTCCTGGTG 
GGCGAGTGCG AAAACATGCA GCTTTCCTAC ATCCACAGCA AGGTCAAGGT CATCTACAAA 
GCCCCTTCTG AAAACTGGGC CATGGAGGGA GGCACAGACC CTGAGACCAC ACTGCCTGGG 
GCTGAGGATG GCAAGACTTA CTTCTTCCAG CTCTGGTACA ACCAGGAGTA CGCAAGGTTT 
GAATCCCCAC CCAAGACCCA GCCGACCGAG GACAACAAGC ACAAGTTCTG CCTATCTTGT 
ATCCGGCTGG CTGAGCTGAG ACAAAAAGAA ATGCCCAAGG TCCTGGAACA AATTGAGGAG 
GTGGATGGCC GGGTCTACTG CAGTTCCATC ACCAAGAATG GTGTTGTCTA CCGACTGGGT 
GACAGTGTGT ACCTTCCTCC CGAGGCCTTT ACTTTCAACA TCAAAGTGGC TAGCCCCGTG 
AAACGCCCAA AGAAGGATCC TGTGAACGAG ACCCTGTACC CTGAGCACTA CCGCAAGTAT 
TCTGACTACA TCAAGGGGAG CAACCTGGAT GCTCCAGAGC CCTATCGCAT CGGTCGGATA 
AAAGAGATCC ACTGTGGCAA GAAGAAAGGC AAGGTCAACG AGGCAGACAT CAAGCTGAGG 
CTCTACAAGT TCTACAGGCC TGAGAATACC CACAGGTCCT ACAACGGATC CTATCACACT 
GACATCAACA TGCTTTACTG GAGCGACGAG GAAGCTGTGG TGAACTTCAG CGACGTGCAG 
GGCCGCTGTA CCGTGGAGTA CGGGGAAGAC CTACTTGAGA GCATCCAGGA TTATTCACAA 
GGGGGCCCTG ACCGCTTCTA CTTCCTCGAG GCCTACAATT CAAAGACCAA GAACTTTGAA 
GACCCACCAA ACCATGCCCG CAGCCCTGGG AACAAAGGGA AAGGGAAGGG GAAAGGGAAG 
GGGAAGGGGA AGCATCAGGT GTCAGAGCCC AAAGAGCCTG AGGCAGCCAT CAAACTGCCC 
AAGCTCCGGA CCCTGGATGT GTTTTCCGGC TGTGGAGGGT TATCGGAAGG ATTCCACCAA 
GCAGGCATCT CGGAAACGCT GTGGGCCATC GAGATGTGGG ACCCGGCAGC CCAGGCATTT 
CGGCTGAACA ACCCCGGCAC CACAGTGTTC ACAGAGGACT GCAACGTGCT TCTTAAGCTG 
GTCATGGCTG GGGAGGTGAC CAACTCTCTG GGCCAAAGGC TGCCACAGAA GGGCGATGTG 
GAGATGCTGT GTGGTGGGCC ACCCTGCCAG GGCTTCAGTG GCATGAACCG CTTCAACTCC 
CGCACTTACT CCAAGTTCAA AAACTCCCTA GTGGTCTCCT TCCTCAGCTA CTGTGACTAC 
TACCGGCCTC GGTTCTTCCT TCTGGAGAAC GTCAGGAACT TCGTGTCCTA CAGACGCTCC 
ATGGTGCTGA AGCTCACACT GCGCTGCCTG GTCCGCATGG GCTACCAGTG CACCTTTGGT 
GTGCTCCAGG CTGGACAGTA TGGCGTGGCC CAGACACGAA GGAGGGCCAT CATCTTGGCT 
GCAGCCCCAG GAGAAAAGCT GCCTCTGTTC CCAGAGCCTC TGCATGTGTT TGCGCCCCGT 
GCCTGCCAGC TGAGCGTTGT GGTGGATGAC AAGAAGTTTG TTAGCAACAT AACGAGGCTG 
AGCTCGGGGC CCTTCCGAAC CATCACCGTG CGAGACACCA TGTCTGACCT CCCCGAGATC 
CAGAATGGAG CCTCGAATTC TGAGATCCCC TACAATGGAG AGCCACTGTC CTGGTTCCAG 
AGGCAGCTGC GAGGATCACA CTACCAGCCC ATCCTCAGGG ACCATATCTG CAAGGACATG 
AGCCCACTGG TGGCTGCCCG CATGCGGCAC ATCCCACTGT TCCCAGGATC AGATTGGCGT 
GACCTGCCCA ACATACAGGT GCGGCTGGGA GATGGCGTCA TAGCCCATAA GCTACAGTAC 
ACCTTTCATG ATGTGAAAAA TGGCTACAGC AGCACCGGTG CCCTGCGTGG AGTCTGTTCC 
TGTGCAGAAG GCAAGGCCTG CGACCCTGAG TCCAGGCAAT TCAGCACCCT CATCCCCTGG 
TGCCTGCCGC ACACTGGGAA CCGGCACAAC CACTGGGCTG GCCTCTACGG GCGTCTGGAG 
TGGGATGGCT TCTTCAGCAC CACTGTCACC AACCCTGAGC CCATGGGCAA GCAGGGTCGG 
GTGCTCCACC CGGAGCAGCA CCGGGTCGTG AGTGTTCGGG AATGTGCCCG CTCCCAGGGC 
TTTCCAGATA GCTACCGGTT CTTCGGCAAC ATCCTGGACA GACACCGGCA GGTGGGTAAT 
GCTGTGCCAC CACCCCTGGC CAAAGCCATT GGCCTGGAGA TTAAGCTCTG CCTGCTGTCC 
AGTGCTCGGG AGAGCGCATC AGCTGCAGTT AAAGCAAAAG AGGAGGCTGC TACCAAGGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnmt1 ( NM_010066.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034196.5 Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 2, mRNA.

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CloneID	OMu15992
Clone ID Related Accession (Same CDS sequence)	NM_010066.4
Accession Version	NM_010066.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	DNA (cytosine-5)-methyltransferase 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF162282.1, AK147143.1 and AC170598.2. On Dec 7, 2010 this sequence version replaced NM_010066.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is one amino acid shorter than isoform 1. This variant has two upstream in-frame AUGs but N-terminal sequencing and western analysis from PMID: 9830015 (Table I and Figure 2) support the use of the third in-frame AUG. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147143.1, X14805.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9830015) ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCAGCGC GAACAGCTCC AGCCCGAGTG CCTGCGCTTG CCTCCCCGGC AGGCTCGCTC 
CCGGACCATG TCCGCAGGCG GCTCAAAGAC TTGGAAAGAG ATGGCTTAAC AGAAAAGGAG 
TGTGTGAGGG AGAAATTAAA CTTACTGCAT GAATTCCTGC AAACAGAAAT AAAAAGCCAG 
TTGTGTGACT TGGAAACCAA ATTACATAAA GAGGAATTAT CTGAGGAAGG CTACCTGGCT 
AAAGTCAAGT CCCTCTTAAA TAAGGATTTG TCCTTGGAGA ACGGAACACA CACTCTCACT 
CAAAAAGCCA ACGGTTGTCC CGCCAACGGG AGCCGGCCAA CCTGGAGAGC AGAAATGGCA 
GACTCAAATA GATCCCCAAG ATCCAGGCCC AAGCCTCGGG GACCCAGGAG AAGCAAGTCG 
GACAGTGACA CCCTTTTTGA AACTTCACCT AGTTCCGTGG CTACGAGGAG AACCACCAGG 
CAGACCACCA TCACGGCTCA CTTCACGAAG GGCCCCACTA AACGGAAACC CAAGGAAGAG 
TCGGAAGAGG GGAACTCGGC TGAGTCGGCT GCAGAGGAGA GAGACCAGGA TAAGAAACGC 
AGAGTTGTAG ACACAGAGAG TGGTGCTGCA GCTGCTGTGG AGAAACTGGA AGAGGTAACA 
GCGGGAACCC AGCTGGGTCC GGAAGAGCCA TGTGAACAGG AAGATGACAA CAGGAGTCTT 
CGACGTCACA CCAGAGAGCT ATCATTGAGG CGGAAATCAA AGGAGGATCC AGACAGAGAA 
GCAAGACCGG AAACTCACTT GGACGAGGAC GAGGACGGAA AAAAGGATAA AAGAAGTTCC 
AGACCCAGGA GCCAGCCCAG AGATCCAGCT GCCAAACGGA GACCCAAGGA AGCAGAGCCA 
GAGCAGGTAG CTCCAGAGAC TCCCGAGGAC AGAGACGAGG ATGAGAGGGA GGAGAAGAGA 
CGAAAAACGA CACGTAAAAA ACTGGAGTCA CACACCGTTC CCGTTCAGAG CAGATCGGAG 
AGAAAAGCCG CTCAAAGCAA AAGTGTGATC CCGAAGATCA ACTCACCAAA GTGCCCCGAG 
TGTGGCCAGC ACCTAGACGA CCCTAACCTG AAGTACCAGC AGCACCCTGA GGATGCTGTG 
GATGAACCCC AGATGTTGAC CAGTGAGAAA CTGTCCATCT ACGACTCCAC CTCGACCTGG 
TTTGATACTT ATGAAGATTC TCCCATGCAT AGGTTCACTT CCTTCAGTGT GTACTGCAGT 
CGCGGGCACC TGTGTCCTGT CGACACCGGT CTCATTGAGA AGAATGTAGA GCTCTACTTT 
TCTGGGTGTG CCAAAGCAAT TCATGACGAG AATCCATCTA TGGAAGGTGG TATTAATGGC 
AAAAACCTCG GGCCAATCAA TCAGTGGTGG CTCAGTGGCT TTGATGGTGG CGAGAAGGTG 
CTCATTGGCT TCTCCACTGC ATTTGCTGAA TACATTTTGA TGGAGCCCAG CAAAGAGTAT 
GAGCCAATAT TTGGGCTGAT GCAGGAGAAA ATTTACATCA GCAAGATTGT TGTTGAGTTC 
CTGCAAAACA ATCCTGATGC TGTATATGAA GACCTGATCA ATAAGATTGA GACCACTGTT 
CCTCCTTCTA CCATTAATGT GAACCGGTTC ACAGAGGACT CCCTCTTACG CCACGCCCAG 
TTTGTAGTGA GCCAGGTAGA GAGTTACGAC GAAGCCAAGG ACGATGATGA GACCCCCATC 
TTCTTGTCTC CCTGTATGAG AGCCCTGATC CATTTGGCTG GTGTCTCCCT GGGACAGAGG 
CGAGCAACAA GGCGCGTCAT GGGTGCTACC AAGGAGAAGG ACAAAGCACC CACGAAAGCC 
ACCACCACCA AGCTGGTCTA TCAGATCTTT GACACTTTCT TCTCAGAGCA GATTGAGAAG 
TATGATAAGG AGGACAAGGA GAATGCCATG AAGCGCCGCC GCTGTGGTGT CTGTGAGGTC 
TGTCAGCAGC CTGAGTGTGG GAAGTGCAAG GCGTGCAAAG ATATGGTGAA GTTTGGTGGC 
ACTGGACGGA GTAAGCAGGC TTGCCTCAAG AGGAGGTGTC CTAACTTGGC GGTGAAGGAG 
GCAGACGACG ATGAAGAGGC TGATGATGAT GTGTCAGAGA TGCCATCACC CAAAAAGCTG 
CATCAGGGGA AGAAGAAGAA GCAGAACAAG GACCGCATCT CCTGGCTTGG GCAGCCTATG 
AAGATTGAAG AGAATAGAAC TTACTATCAG AAGGTGAGCA TCGATGAGGA GATGCTAGAG 
GTGGGCGACT GCGTCTCGGT CATTCCAGAT GATTCCTCCA AACCACTCTA TCTAGCCAGG 
GTCACAGCTC TGTGGGAAGA CAAAAATGGT CAGATGATGT TCCATGCGCA CTGGTTCTGC 
GCTGGGACAG ACACAGTCCT GGGAGCCACC TCCGACCCCC TGGAACTGTT CCTGGTGGGC 
GAGTGCGAAA ACATGCAGCT TTCCTACATC CACAGCAAGG TCAAGGTCAT CTACAAAGCC 
CCTTCTGAAA ACTGGGCCAT GGAGGGAGGC ACAGACCCTG AGACCACACT GCCTGGGGCT 
GAGGATGGCA AGACTTACTT CTTCCAGCTC TGGTACAACC AGGAGTACGC AAGGTTTGAA 
TCCCCACCCA AGACCCAGCC GACCGAGGAC AACAAGCACA AGTTCTGCCT ATCTTGTATC 
CGGCTGGCTG AGCTGAGACA AAAAGAAATG CCCAAGGTCC TGGAACAAAT TGAGGAGGTG 
GATGGCCGGG TCTACTGCAG TTCCATCACC AAGAATGGTG TTGTCTACCG ACTGGGTGAC 
AGTGTGTACC TTCCTCCCGA GGCCTTTACT TTCAACATCA AAGTGGCTAG CCCCGTGAAA 
CGCCCAAAGA AGGATCCTGT GAACGAGACC CTGTACCCTG AGCACTACCG CAAGTATTCT 
GACTACATCA AGGGGAGCAA CCTGGATGCT CCAGAGCCCT ATCGCATCGG TCGGATAAAA 
GAGATCCACT GTGGCAAGAA GAAAGGCAAG GTCAACGAGG CAGACATCAA GCTGAGGCTC 
TACAAGTTCT ACAGGCCTGA GAATACCCAC AGGTCCTACA ACGGATCCTA TCACACTGAC 
ATCAACATGC TTTACTGGAG CGACGAGGAA GCTGTGGTGA ACTTCAGCGA CGTGCAGGGC 
CGCTGTACCG TGGAGTACGG GGAAGACCTA CTTGAGAGCA TCCAGGATTA TTCACAAGGG 
GGCCCTGACC GCTTCTACTT CCTCGAGGCC TACAATTCAA AGACCAAGAA CTTTGAAGAC 
CCACCAAACC ATGCCCGCAG CCCTGGGAAC AAAGGGAAAG GGAAGGGGAA AGGGAAGGGG 
AAGGGGAAGC ATCAGGTGTC AGAGCCCAAA GAGCCTGAGG CAGCCATCAA ACTGCCCAAG 
CTCCGGACCC TGGATGTGTT TTCCGGCTGT GGAGGGTTAT CGGAAGGATT CCACCAAGCA 
GGCATCTCGG AAACGCTGTG GGCCATCGAG ATGTGGGACC CGGCAGCCCA GGCATTTCGG 
CTGAACAACC CCGGCACCAC AGTGTTCACA GAGGACTGCA ACGTGCTTCT TAAGCTGGTC 
ATGGCTGGGG AGGTGACCAA CTCTCTGGGC CAAAGGCTGC CACAGAAGGG CGATGTGGAG 
ATGCTGTGTG GTGGGCCACC CTGCCAGGGC TTCAGTGGCA TGAACCGCTT CAACTCCCGC 
ACTTACTCCA AGTTCAAAAA CTCCCTAGTG GTCTCCTTCC TCAGCTACTG TGACTACTAC 
CGGCCTCGGT TCTTCCTTCT GGAGAACGTC AGGAACTTCG TGTCCTACAG ACGCTCCATG 
GTGCTGAAGC TCACACTGCG CTGCCTGGTC CGCATGGGCT ACCAGTGCAC CTTTGGTGTG 
CTCCAGGCTG GACAGTATGG CGTGGCCCAG ACACGAAGGA GGGCCATCAT CTTGGCTGCA 
GCCCCAGGAG AAAAGCTGCC TCTGTTCCCA GAGCCTCTGC ATGTGTTTGC GCCCCGTGCC 
TGCCAGCTGA GCGTTGTGGT GGATGACAAG AAGTTTGTTA GCAACATAAC GAGGCTGAGC 
TCGGGGCCCT TCCGAACCAT CACCGTGCGA GACACCATGT CTGACCTCCC CGAGATCCAG 
AATGGAGCCT CGAATTCTGA GATCCCCTAC AATGGAGAGC CACTGTCCTG GTTCCAGAGG 
CAGCTGCGAG GATCACACTA CCAGCCCATC CTCAGGGACC ATATCTGCAA GGACATGAGC 
CCACTGGTGG CTGCCCGCAT GCGGCACATC CCACTGTTCC CAGGATCAGA TTGGCGTGAC 
CTGCCCAACA TACAGGTGCG GCTGGGAGAT GGCGTCATAG CCCATAAGCT ACAGTACACC 
TTTCATGATG TGAAAAATGG CTACAGCAGC ACCGGTGCCC TGCGTGGAGT CTGTTCCTGT 
GCAGAAGGCA AGGCCTGCGA CCCTGAGTCC AGGCAATTCA GCACCCTCAT CCCCTGGTGC 
CTGCCGCACA CTGGGAACCG GCACAACCAC TGGGCTGGCC TCTACGGGCG TCTGGAGTGG 
GATGGCTTCT TCAGCACCAC TGTCACCAAC CCTGAGCCCA TGGGCAAGCA GGGTCGGGTG 
CTCCACCCGG AGCAGCACCG GGTCGTGAGT GTTCGGGAAT GTGCCCGCTC CCAGGGCTTT 
CCAGATAGCT ACCGGTTCTT CGGCAACATC CTGGACAGAC ACCGGCAGGT GGGTAATGCT 
GTGCCACCAC CCCTGGCCAA AGCCATTGGC CTGGAGATTA AGCTCTGCCT GCTGTCCAGT 
GCTCGGGAGA GCGCATCAGC TGCAGTTAAA GCAAAAGAGG AGGCTGCTAC CAAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034196.5
CDS	193..5052
Translation	MPARTAPARVPALASPAGSLPDHVRRRLKDLERDGLTEKECVREKLNLLHEFLQTEIKSQLCDLETKLHKEELSEEGYLAKVKSLLNKDLSLENGTHTLTQKANGCPANGSRPTWRAEMADSNRSPRSRPKPRGPRRSKSDSDTLFETSPSSVATRRTTRQTTITAHFTKGPTKRKPKEESEEGNSAESAAEERDQDKKRRVVDTESGAAAAVEKLEEVTAGTQLGPEEPCEQEDDNRSLRRHTRELSLRRKSKEDPDREARPETHLDEDEDGKKDKRSSRPRSQPRDPAAKRRPKEAEPEQVAPETPEDRDEDEREEKRRKTTRKKLESHTVPVQSRSERKAAQSKSVIPKINSPKCPECGQHLDDPNLKYQQHPEDAVDEPQMLTSEKLSIYDSTSTWFDTYEDSPMHRFTSFSVYCSRGHLCPVDTGLIEKNVELYFSGCAKAIHDENPSMEGGINGKNLGPINQWWLSGFDGGEKVLIGFSTAFAEYILMEPSKEYEPIFGLMQEKIYISKIVVEFLQNNPDAVYEDLINKIETTVPPSTINVNRFTEDSLLRHAQFVVSQVESYDEAKDDDETPIFLSPCMRALIHLAGVSLGQRRATRRVMGATKEKDKAPTKATTTKLVYQIFDTFFSEQIEKYDKEDKENAMKRRRCGVCEVCQQPECGKCKACKDMVKFGGTGRSKQACLKRRCPNLAVKEADDDEEADDDVSEMPSPKKLHQGKKKKQNKDRISWLGQPMKIEENRTYYQKVSIDEEMLEVGDCVSVIPDDSSKPLYLARVTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYIHSKVKVIYKAPSENWAMEGGTDPETTLPGAEDGKTYFFQLWYNQEYARFESPPKTQPTEDNKHKFCLSCIRLAELRQKEMPKVLEQIEEVDGRVYCSSITKNGVVYRLGDSVYLPPEAFTFNIKVASPVKRPKKDPVNETLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPENTHRSYNGSYHTDINMLYWSDEEAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSKTKNFEDPPNHARSPGNKGKGKGKGKGKGKHQVSEPKEPEAAIKLPKLRTLDVFSGCGGLSEGFHQAGISETLWAIEMWDPAAQAFRLNNPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEIQNGASNSEIPYNGEPLSWFQRQLRGSHYQPILRDHICKDMSPLVAARMRHIPLFPGSDWRDLPNIQVRLGDGVIAHKLQYTFHDVKNGYSSTGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDSYRFFGNILDRHRQVGNAVPPPLAKAIGLEIKLCLLSSARESASAAVKAKEEAATKD

Target ORF information:

RefSeq Version	NM_010066.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010066.4

ORF Insert Sequence:

ATGCCAGCGC GAACAGCTCC AGCCCGAGTG CCTGCGCTTG CCTCCCCGGC AGGCTCGCTC 
CCGGACCATG TCCGCAGGCG GCTCAAAGAC TTGGAAAGAG ATGGCTTAAC AGAAAAGGAG 
TGTGTGAGGG AGAAATTAAA CTTACTGCAT GAATTCCTGC AAACAGAAAT AAAAAGCCAG 
TTGTGTGACT TGGAAACCAA ATTACATAAA GAGGAATTAT CTGAGGAAGG CTACCTGGCT 
AAAGTCAAGT CCCTCTTAAA TAAGGATTTG TCCTTGGAGA ACGGAACACA CACTCTCACT 
CAAAAAGCCA ACGGTTGTCC CGCCAACGGG AGCCGGCCAA CCTGGAGAGC AGAAATGGCA 
GACTCAAATA GATCCCCAAG ATCCAGGCCC AAGCCTCGGG GACCCAGGAG AAGCAAGTCG 
GACAGTGACA CCCTTTTTGA AACTTCACCT AGTTCCGTGG CTACGAGGAG AACCACCAGG 
CAGACCACCA TCACGGCTCA CTTCACGAAG GGCCCCACTA AACGGAAACC CAAGGAAGAG 
TCGGAAGAGG GGAACTCGGC TGAGTCGGCT GCAGAGGAGA GAGACCAGGA TAAGAAACGC 
AGAGTTGTAG ACACAGAGAG TGGTGCTGCA GCTGCTGTGG AGAAACTGGA AGAGGTAACA 
GCGGGAACCC AGCTGGGTCC GGAAGAGCCA TGTGAACAGG AAGATGACAA CAGGAGTCTT 
CGACGTCACA CCAGAGAGCT ATCATTGAGG CGGAAATCAA AGGAGGATCC AGACAGAGAA 
GCAAGACCGG AAACTCACTT GGACGAGGAC GAGGACGGAA AAAAGGATAA AAGAAGTTCC 
AGACCCAGGA GCCAGCCCAG AGATCCAGCT GCCAAACGGA GACCCAAGGA AGCAGAGCCA 
GAGCAGGTAG CTCCAGAGAC TCCCGAGGAC AGAGACGAGG ATGAGAGGGA GGAGAAGAGA 
CGAAAAACGA CACGTAAAAA ACTGGAGTCA CACACCGTTC CCGTTCAGAG CAGATCGGAG 
AGAAAAGCCG CTCAAAGCAA AAGTGTGATC CCGAAGATCA ACTCACCAAA GTGCCCCGAG 
TGTGGCCAGC ACCTAGACGA CCCTAACCTG AAGTACCAGC AGCACCCTGA GGATGCTGTG 
GATGAACCCC AGATGTTGAC CAGTGAGAAA CTGTCCATCT ACGACTCCAC CTCGACCTGG 
TTTGATACTT ATGAAGATTC TCCCATGCAT AGGTTCACTT CCTTCAGTGT GTACTGCAGT 
CGCGGGCACC TGTGTCCTGT CGACACCGGT CTCATTGAGA AGAATGTAGA GCTCTACTTT 
TCTGGGTGTG CCAAAGCAAT TCATGACGAG AATCCATCTA TGGAAGGTGG TATTAATGGC 
AAAAACCTCG GGCCAATCAA TCAGTGGTGG CTCAGTGGCT TTGATGGTGG CGAGAAGGTG 
CTCATTGGCT TCTCCACTGC ATTTGCTGAA TACATTTTGA TGGAGCCCAG CAAAGAGTAT 
GAGCCAATAT TTGGGCTGAT GCAGGAGAAA ATTTACATCA GCAAGATTGT TGTTGAGTTC 
CTGCAAAACA ATCCTGATGC TGTATATGAA GACCTGATCA ATAAGATTGA GACCACTGTT 
CCTCCTTCTA CCATTAATGT GAACCGGTTC ACAGAGGACT CCCTCTTACG CCACGCCCAG 
TTTGTAGTGA GCCAGGTAGA GAGTTACGAC GAAGCCAAGG ACGATGATGA GACCCCCATC 
TTCTTGTCTC CCTGTATGAG AGCCCTGATC CATTTGGCTG GTGTCTCCCT GGGACAGAGG 
CGAGCAACAA GGCGCGTCAT GGGTGCTACC AAGGAGAAGG ACAAAGCACC CACGAAAGCC 
ACCACCACCA AGCTGGTCTA TCAGATCTTT GACACTTTCT TCTCAGAGCA GATTGAGAAG 
TATGATAAGG AGGACAAGGA GAATGCCATG AAGCGCCGCC GCTGTGGTGT CTGTGAGGTC 
TGTCAGCAGC CTGAGTGTGG GAAGTGCAAG GCGTGCAAAG ATATGGTGAA GTTTGGTGGC 
ACTGGACGGA GTAAGCAGGC TTGCCTCAAG AGGAGGTGTC CTAACTTGGC GGTGAAGGAG 
GCAGACGACG ATGAAGAGGC TGATGATGAT GTGTCAGAGA TGCCATCACC CAAAAAGCTG 
CATCAGGGGA AGAAGAAGAA GCAGAACAAG GACCGCATCT CCTGGCTTGG GCAGCCTATG 
AAGATTGAAG AGAATAGAAC TTACTATCAG AAGGTGAGCA TCGATGAGGA GATGCTAGAG 
GTGGGCGACT GCGTCTCGGT CATTCCAGAT GATTCCTCCA AACCACTCTA TCTAGCCAGG 
GTCACAGCTC TGTGGGAAGA CAAAAATGGT CAGATGATGT TCCATGCGCA CTGGTTCTGC 
GCTGGGACAG ACACAGTCCT GGGAGCCACC TCCGACCCCC TGGAACTGTT CCTGGTGGGC 
GAGTGCGAAA ACATGCAGCT TTCCTACATC CACAGCAAGG TCAAGGTCAT CTACAAAGCC 
CCTTCTGAAA ACTGGGCCAT GGAGGGAGGC ACAGACCCTG AGACCACACT GCCTGGGGCT 
GAGGATGGCA AGACTTACTT CTTCCAGCTC TGGTACAACC AGGAGTACGC AAGGTTTGAA 
TCCCCACCCA AGACCCAGCC GACCGAGGAC AACAAGCACA AGTTCTGCCT ATCTTGTATC 
CGGCTGGCTG AGCTGAGACA AAAAGAAATG CCCAAGGTCC TGGAACAAAT TGAGGAGGTG 
GATGGCCGGG TCTACTGCAG TTCCATCACC AAGAATGGTG TTGTCTACCG ACTGGGTGAC 
AGTGTGTACC TTCCTCCCGA GGCCTTTACT TTCAACATCA AAGTGGCTAG CCCCGTGAAA 
CGCCCAAAGA AGGATCCTGT GAACGAGACC CTGTACCCTG AGCACTACCG CAAGTATTCT 
GACTACATCA AGGGGAGCAA CCTGGATGCT CCAGAGCCCT ATCGCATCGG TCGGATAAAA 
GAGATCCACT GTGGCAAGAA GAAAGGCAAG GTCAACGAGG CAGACATCAA GCTGAGGCTC 
TACAAGTTCT ACAGGCCTGA GAATACCCAC AGGTCCTACA ACGGATCCTA TCACACTGAC 
ATCAACATGC TTTACTGGAG CGACGAGGAA GCTGTGGTGA ACTTCAGCGA CGTGCAGGGC 
CGCTGTACCG TGGAGTACGG GGAAGACCTA CTTGAGAGCA TCCAGGATTA TTCACAAGGG 
GGCCCTGACC GCTTCTACTT CCTCGAGGCC TACAATTCAA AGACCAAGAA CTTTGAAGAC 
CCACCAAACC ATGCCCGCAG CCCTGGGAAC AAAGGGAAAG GGAAGGGGAA AGGGAAGGGG 
AAGGGGAAGC ATCAGGTGTC AGAGCCCAAA GAGCCTGAGG CAGCCATCAA ACTGCCCAAG 
CTCCGGACCC TGGATGTGTT TTCCGGCTGT GGAGGGTTAT CGGAAGGATT CCACCAAGCA 
GGCATCTCGG AAACGCTGTG GGCCATCGAG ATGTGGGACC CGGCAGCCCA GGCATTTCGG 
CTGAACAACC CCGGCACCAC AGTGTTCACA GAGGACTGCA ACGTGCTTCT TAAGCTGGTC 
ATGGCTGGGG AGGTGACCAA CTCTCTGGGC CAAAGGCTGC CACAGAAGGG CGATGTGGAG 
ATGCTGTGTG GTGGGCCACC CTGCCAGGGC TTCAGTGGCA TGAACCGCTT CAACTCCCGC 
ACTTACTCCA AGTTCAAAAA CTCCCTAGTG GTCTCCTTCC TCAGCTACTG TGACTACTAC 
CGGCCTCGGT TCTTCCTTCT GGAGAACGTC AGGAACTTCG TGTCCTACAG ACGCTCCATG 
GTGCTGAAGC TCACACTGCG CTGCCTGGTC CGCATGGGCT ACCAGTGCAC CTTTGGTGTG 
CTCCAGGCTG GACAGTATGG CGTGGCCCAG ACACGAAGGA GGGCCATCAT CTTGGCTGCA 
GCCCCAGGAG AAAAGCTGCC TCTGTTCCCA GAGCCTCTGC ATGTGTTTGC GCCCCGTGCC 
TGCCAGCTGA GCGTTGTGGT GGATGACAAG AAGTTTGTTA GCAACATAAC GAGGCTGAGC 
TCGGGGCCCT TCCGAACCAT CACCGTGCGA GACACCATGT CTGACCTCCC CGAGATCCAG 
AATGGAGCCT CGAATTCTGA GATCCCCTAC AATGGAGAGC CACTGTCCTG GTTCCAGAGG 
CAGCTGCGAG GATCACACTA CCAGCCCATC CTCAGGGACC ATATCTGCAA GGACATGAGC 
CCACTGGTGG CTGCCCGCAT GCGGCACATC CCACTGTTCC CAGGATCAGA TTGGCGTGAC 
CTGCCCAACA TACAGGTGCG GCTGGGAGAT GGCGTCATAG CCCATAAGCT ACAGTACACC 
TTTCATGATG TGAAAAATGG CTACAGCAGC ACCGGTGCCC TGCGTGGAGT CTGTTCCTGT 
GCAGAAGGCA AGGCCTGCGA CCCTGAGTCC AGGCAATTCA GCACCCTCAT CCCCTGGTGC 
CTGCCGCACA CTGGGAACCG GCACAACCAC TGGGCTGGCC TCTACGGGCG TCTGGAGTGG 
GATGGCTTCT TCAGCACCAC TGTCACCAAC CCTGAGCCCA TGGGCAAGCA GGGTCGGGTG 
CTCCACCCGG AGCAGCACCG GGTCGTGAGT GTTCGGGAAT GTGCCCGCTC CCAGGGCTTT 
CCAGATAGCT ACCGGTTCTT CGGCAACATC CTGGACAGAC ACCGGCAGGT GGGTAATGCT 
GTGCCACCAC CCCTGGCCAA AGCCATTGGC CTGGAGATTA AGCTCTGCCT GCTGTCCAGT 
GCTCGGGAGA GCGCATCAGC TGCAGTTAAA GCAAAAGAGG AGGCTGCTAC CAAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnmt1 ( XM_006509988.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510051.1 Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant X1, mRNA.

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CloneID	OMu41988
Clone ID Related Accession (Same CDS sequence)	XM_006509988.2
Accession Version	XM_006509988.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA (cytosine-5)-methyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509988.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCACTC CCTTCGGGCA TAGCATGGTC TTCCCCCACT CTCTTGCCCT GTGTGGTACA 
TGCTGCTTCC GCTTGCGCCG CCCCCTCCCA ATTGGTTTCC GCGCGCGCGA AAAAGCCGGG 
GTCTCGTTCA GAGCTGTTCT GTCGTCTGCA ACCTGCAAGA TGCCAGCGCG AACAGCTCCA 
GCCCGAGTGC CTGCGCTTGC CTCCCCGGCA GGCTCGCTCC CGGACCATGT CCGCAGGCGG 
CTCAAAGACT TGGAAAGAGA TGGCTTAACA GAAAAGGAGT GTGTGAGGGA GAAATTAAAC 
TTACTGCATG AATTCCTGCA AACAGAAATA AAAAGCCAGT TGTGTGACTT GGAAACCAAA 
TTACATAAAG AGGAATTATC TGAGGAAGGC TACCTGGCTA AAGTCAAGTC CCTCTTAAAT 
AAGGATTTGT CCTTGGAGAA CGGAACACAC ACTCTCACTC AAAAAGCCAA CGGTTGTCCC 
GCCAACGGGA GCCGGCCAAC CTGGAGAGCA GAAATGGCAG ACTCAAATAG ATCCCCAAGA 
TCCAGGCCCA AGCCTCGGGG ACCCAGGAGA AGCAAGTCGG ACAGTGACAC CCTTTGTAAG 
GACACGAGAC ATACTGTTGA AACTTCACCT AGTTCCGTGG CTACGAGGAG AACCACCAGG 
CAGACCACCA TCACGGCTCA CTTCACGAAG GGCCCCACTA AACGGAAACC CAAGGAAGAG 
TCGGAAGAGG GGAACTCGGC TGAGTCGGCT GCAGAGGAGA GAGACCAGGA TAAGAAACGC 
AGAGTTGTAG ACACAGAGAG TGGTGCTGCA GCTGCTGTGG AGAAACTGGA AGAGGTAACA 
GCGGGAACCC AGCTGGGTCC GGAAGAGCCA TGTGAACAGG AAGATGACAA CAGGAGTCTT 
CGACGTCACA CCAGAGAGCT ATCATTGAGG CGGAAATCAA AGGAGGATCC AGACAGAGAA 
GCAAGACCGG AAACTCACTT GGACGAGGAC GAGGACGGAA AAAAGGATAA AAGAAGTTCC 
AGACCCAGGA GCCAGCCCAG AGATCCAGCT GCCAAACGGA GACCCAAGGA AGCAGAGCCA 
GAGCAGGTAG CTCCAGAGAC TCCCGAGGAC AGAGACGAGG ATGAGAGGGA GGAGAAGAGA 
CGAAAAACGA CACGTAAAAA ACTGGAGTCA CACACCGTTC CCGTTCAGAG CAGATCGGAG 
AGAAAAGCCG CTCAAAGCAA AAGTGTGATC CCGAAGATCA ACTCACCAAA GTGCCCCGAG 
TGTGGCCAGC ACCTAGACGA CCCTAACCTG AAGTACCAGC AGCACCCTGA GGATGCTGTG 
GATGAACCCC AGATGTTGAC CAGTGAGAAA CTGTCCATCT ACGACTCCAC CTCGACCTGG 
TTTGATACTT ATGAAGATTC TCCCATGCAT AGGTTCACTT CCTTCAGTGT GTACTGCAGT 
CGCGGGCACC TGTGTCCTGT CGACACCGGT CTCATTGAGA AGAATGTAGA GCTCTACTTT 
TCTGGGTGTG CCAAAGCAAT TCATGACGAG AATCCATCTA TGGAAGGTGG TATTAATGGC 
AAAAACCTCG GGCCAATCAA TCAGTGGTGG CTCAGTGGCT TTGATGGTGG CGAGAAGGTG 
CTCATTGGCT TCTCCACTGC ATTTGCTGAA TACATTTTGA TGGAGCCCAG CAAAGAGTAT 
GAGCCAATAT TTGGGCTGAT GCAGGAGAAA ATTTACATCA GCAAGATTGT TGTTGAGTTC 
CTGCAAAACA ATCCTGATGC TGTATATGAA GACCTGATCA ATAAGATTGA GACCACTGTT 
CCTCCTTCTA CCATTAATGT GAACCGGTTC ACAGAGGACT CCCTCTTACG CCACGCCCAG 
TTTGTAGTGA GCCAGGTAGA GAGTTACGAC GAAGCCAAGG ACGATGATGA GACCCCCATC 
TTCTTGTCTC CCTGTATGAG AGCCCTGATC CATTTGGCTG GTGTCTCCCT GGGACAGAGG 
CGAGCAACAA GGCGCGTCAT GGGTGCTACC AAGGAGAAGG ACAAAGCACC CACGAAAGCC 
ACCACCACCA AGCTGGTCTA TCAGATCTTT GACACTTTCT TCTCAGAGCA GATTGAGAAG 
TATGATAAGG AGGACAAGGA GAATGCCATG AAGCGCCGCC GCTGTGGTGT CTGTGAGGTC 
TGTCAGCAGC CTGAGTGTGG GAAGTGCAAG GCGTGCAAAG ATATGGTGAA GTTTGGTGGC 
ACTGGACGGA GTAAGCAGGC TTGCCTCAAG AGGAGGTGTC CTAACTTGGC GGTGAAGGAG 
GCAGACGACG ATGAAGAGGC TGATGATGAT GTGTCAGAGA TGCCATCACC CAAAAAGCTG 
CATCAGGGGA AGAAGAAGAA GCAGAACAAG GACCGCATCT CCTGGCTTGG GCAGCCTATG 
AAGATTGAAG AGAATAGAAC TTACTATCAG AAGGTGAGCA TCGATGAGGA GATGCTAGAG 
GTGGGCGACT GCGTCTCGGT CATTCCAGAT GATTCCTCCA AACCACTCTA TCTAGCCAGG 
GTCACAGCTC TGTGGGAAGA CAAAAATGGT CAGATGATGT TCCATGCGCA CTGGTTCTGC 
GCTGGGACAG ACACAGTCCT GGGAGCCACC TCCGACCCCC TGGAACTGTT CCTGGTGGGC 
GAGTGCGAAA ACATGCAGCT TTCCTACATC CACAGCAAGG TCAAGGTCAT CTACAAAGCC 
CCTTCTGAAA ACTGGGCCAT GGAGGGAGGC ACAGACCCTG AGACCACACT GCCTGGGGCT 
GAGGATGGCA AGACTTACTT CTTCCAGCTC TGGTACAACC AGGAGTACGC AAGGTTTGAA 
TCCCCACCCA AGACCCAGCC GACCGAGGAC AACAAGCACA AGTTCTGCCT ATCTTGTATC 
CGGCTGGCTG AGCTGAGACA AAAAGAAATG CCCAAGGTCC TGGAACAAAT TGAGGAGGTG 
GATGGCCGGG TCTACTGCAG TTCCATCACC AAGAATGGTG TTGTCTACCG ACTGGGTGAC 
AGTGTGTACC TTCCTCCCGA GGCCTTTACT TTCAACATCA AAGTGGCTAG CCCCGTGAAA 
CGCCCAAAGA AGGATCCTGT GAACGAGACC CTGTACCCTG AGCACTACCG CAAGTATTCT 
GACTACATCA AGGGGAGCAA CCTGGATGCT CCAGAGCCCT ATCGCATCGG TCGGATAAAA 
GAGATCCACT GTGGCAAGAA GAAAGGCAAG GTCAACGAGG CAGACATCAA GCTGAGGCTC 
TACAAGTTCT ACAGGCCTGA GAATACCCAC AGGTCCTACA ACGGATCCTA TCACACTGAC 
ATCAACATGC TTTACTGGAG CGACGAGGAA GCTGTGGTGA ACTTCAGCGA CGTGCAGGGC 
CGCTGTACCG TGGAGTACGG GGAAGACCTA CTTGAGAGCA TCCAGGATTA TTCACAAGGG 
GGCCCTGACC GCTTCTACTT CCTCGAGGCC TACAATTCAA AGACCAAGAA CTTTGAAGAC 
CCACCAAACC ATGCCCGCAG CCCTGGGAAC AAAGGGAAAG GGAAGGGGAA AGGGAAGGGG 
AAGGGGAAGC ATCAGGTGTC AGAGCCCAAA GAGCCTGAGG CAGCCATCAA ACTGCCCAAG 
CTCCGGACCC TGGATGTGTT TTCCGGCTGT GGAGGGTTAT CGGAAGGATT CCACCAAGCA 
GGCATCTCGG AAACGCTGTG GGCCATCGAG ATGTGGGACC CGGCAGCCCA GGCATTTCGG 
CTGAACAACC CCGGCACCAC AGTGTTCACA GAGGACTGCA ACGTGCTTCT TAAGCTGGTC 
ATGGCTGGGG AGGTGACCAA CTCTCTGGGC CAAAGGCTGC CACAGAAGGG CGATGTGGAG 
ATGCTGTGTG GTGGGCCACC CTGCCAGGGC TTCAGTGGCA TGAACCGCTT CAACTCCCGC 
ACTTACTCCA AGTTCAAAAA CTCCCTAGTG GTCTCCTTCC TCAGCTACTG TGACTACTAC 
CGGCCTCGGT TCTTCCTTCT GGAGAACGTC AGGAACTTCG TGTCCTACAG ACGCTCCATG 
GTGCTGAAGC TCACACTGCG CTGCCTGGTC CGCATGGGCT ACCAGTGCAC CTTTGGTGTG 
CTCCAGGCTG GACAGTATGG CGTGGCCCAG ACACGAAGGA GGGCCATCAT CTTGGCTGCA 
GCCCCAGGAG AAAAGCTGCC TCTGTTCCCA GAGCCTCTGC ATGTGTTTGC GCCCCGTGCC 
TGCCAGCTGA GCGTTGTGGT GGATGACAAG AAGTTTGTTA GCAACATAAC GAGGCTGAGC 
TCGGGGCCCT TCCGAACCAT CACCGTGCGA GACACCATGT CTGACCTCCC CGAGATCCAG 
AATGGAGCCT CGAATTCTGA GATCCCCTAC AATGGAGAGC CACTGTCCTG GTTCCAGAGG 
CAGCTGCGAG GATCACACTA CCAGCCCATC CTCAGGGACC ATATCTGCAA GGACATGAGC 
CCACTGGTGG CTGCCCGCAT GCGGCACATC CCACTGTTCC CAGGATCAGA TTGGCGTGAC 
CTGCCCAACA TACAGGTGCG GCTGGGAGAT GGCGTCATAG CCCATAAGCT ACAGTACACC 
TTTCATGATG TGAAAAATGG CTACAGCAGC ACCGGTGCCC TGCGTGGAGT CTGTTCCTGT 
GCAGAAGGCA AGGCCTGCGA CCCTGAGTCC AGGCAATTCA GCACCCTCAT CCCCTGGTGC 
CTGCCGCACA CTGGGAACCG GCACAACCAC TGGGCTGGCC TCTACGGGCG TCTGGAGTGG 
GATGGCTTCT TCAGCACCAC TGTCACCAAC CCTGAGCCCA TGGGCAAGCA GGGTCGGGTG 
CTCCACCCGG AGCAGCACCG GGTCGTGAGT GTTCGGGAAT GTGCCCGCTC CCAGGGCTTT 
CCAGATAGCT ACCGGTTCTT CGGCAACATC CTGGACAGAC ACCGGCAGGT GGGTAATGCT 
GTGCCACCAC CCCTGGCCAA AGCCATTGGC CTGGAGATTA AGCTCTGCCT GCTGTCCAGT 
GCTCGGGAGA GCGCATCAGC TGCAGTTAAA GCAAAAGAGG AGGCTGCTAC CAAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510051.1
CDS	29..5068
Translation	MRTPFGHSMVFPHSLALCGTCCFRLRRPLPIGFRAREKAGVSFRAVLSSATCKMPARTAPARVPALASPAGSLPDHVRRRLKDLERDGLTEKECVREKLNLLHEFLQTEIKSQLCDLETKLHKEELSEEGYLAKVKSLLNKDLSLENGTHTLTQKANGCPANGSRPTWRAEMADSNRSPRSRPKPRGPRRSKSDSDTLCKDTRHTVETSPSSVATRRTTRQTTITAHFTKGPTKRKPKEESEEGNSAESAAEERDQDKKRRVVDTESGAAAAVEKLEEVTAGTQLGPEEPCEQEDDNRSLRRHTRELSLRRKSKEDPDREARPETHLDEDEDGKKDKRSSRPRSQPRDPAAKRRPKEAEPEQVAPETPEDRDEDEREEKRRKTTRKKLESHTVPVQSRSERKAAQSKSVIPKINSPKCPECGQHLDDPNLKYQQHPEDAVDEPQMLTSEKLSIYDSTSTWFDTYEDSPMHRFTSFSVYCSRGHLCPVDTGLIEKNVELYFSGCAKAIHDENPSMEGGINGKNLGPINQWWLSGFDGGEKVLIGFSTAFAEYILMEPSKEYEPIFGLMQEKIYISKIVVEFLQNNPDAVYEDLINKIETTVPPSTINVNRFTEDSLLRHAQFVVSQVESYDEAKDDDETPIFLSPCMRALIHLAGVSLGQRRATRRVMGATKEKDKAPTKATTTKLVYQIFDTFFSEQIEKYDKEDKENAMKRRRCGVCEVCQQPECGKCKACKDMVKFGGTGRSKQACLKRRCPNLAVKEADDDEEADDDVSEMPSPKKLHQGKKKKQNKDRISWLGQPMKIEENRTYYQKVSIDEEMLEVGDCVSVIPDDSSKPLYLARVTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYIHSKVKVIYKAPSENWAMEGGTDPETTLPGAEDGKTYFFQLWYNQEYARFESPPKTQPTEDNKHKFCLSCIRLAELRQKEMPKVLEQIEEVDGRVYCSSITKNGVVYRLGDSVYLPPEAFTFNIKVASPVKRPKKDPVNETLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPENTHRSYNGSYHTDINMLYWSDEEAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSKTKNFEDPPNHARSPGNKGKGKGKGKGKGKHQVSEPKEPEAAIKLPKLRTLDVFSGCGGLSEGFHQAGISETLWAIEMWDPAAQAFRLNNPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEIQNGASNSEIPYNGEPLSWFQRQLRGSHYQPILRDHICKDMSPLVAARMRHIPLFPGSDWRDLPNIQVRLGDGVIAHKLQYTFHDVKNGYSSTGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDSYRFFGNILDRHRQVGNAVPPPLAKAIGLEIKLCLLSSARESASAAVKAKEEAATKD

Target ORF information:

RefSeq Version	XM_006509988.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509988.2

ORF Insert Sequence:

ATGCGCACTC CCTTCGGGCA TAGCATGGTC TTCCCCCACT CTCTTGCCCT GTGTGGTACA 
TGCTGCTTCC GCTTGCGCCG CCCCCTCCCA ATTGGTTTCC GCGCGCGCGA AAAAGCCGGG 
GTCTCGTTCA GAGCTGTTCT GTCGTCTGCA ACCTGCAAGA TGCCAGCGCG AACAGCTCCA 
GCCCGAGTGC CTGCGCTTGC CTCCCCGGCA GGCTCGCTCC CGGACCATGT CCGCAGGCGG 
CTCAAAGACT TGGAAAGAGA TGGCTTAACA GAAAAGGAGT GTGTGAGGGA GAAATTAAAC 
TTACTGCATG AATTCCTGCA AACAGAAATA AAAAGCCAGT TGTGTGACTT GGAAACCAAA 
TTACATAAAG AGGAATTATC TGAGGAAGGC TACCTGGCTA AAGTCAAGTC CCTCTTAAAT 
AAGGATTTGT CCTTGGAGAA CGGAACACAC ACTCTCACTC AAAAAGCCAA CGGTTGTCCC 
GCCAACGGGA GCCGGCCAAC CTGGAGAGCA GAAATGGCAG ACTCAAATAG ATCCCCAAGA 
TCCAGGCCCA AGCCTCGGGG ACCCAGGAGA AGCAAGTCGG ACAGTGACAC CCTTTGTAAG 
GACACGAGAC ATACTGTTGA AACTTCACCT AGTTCCGTGG CTACGAGGAG AACCACCAGG 
CAGACCACCA TCACGGCTCA CTTCACGAAG GGCCCCACTA AACGGAAACC CAAGGAAGAG 
TCGGAAGAGG GGAACTCGGC TGAGTCGGCT GCAGAGGAGA GAGACCAGGA TAAGAAACGC 
AGAGTTGTAG ACACAGAGAG TGGTGCTGCA GCTGCTGTGG AGAAACTGGA AGAGGTAACA 
GCGGGAACCC AGCTGGGTCC GGAAGAGCCA TGTGAACAGG AAGATGACAA CAGGAGTCTT 
CGACGTCACA CCAGAGAGCT ATCATTGAGG CGGAAATCAA AGGAGGATCC AGACAGAGAA 
GCAAGACCGG AAACTCACTT GGACGAGGAC GAGGACGGAA AAAAGGATAA AAGAAGTTCC 
AGACCCAGGA GCCAGCCCAG AGATCCAGCT GCCAAACGGA GACCCAAGGA AGCAGAGCCA 
GAGCAGGTAG CTCCAGAGAC TCCCGAGGAC AGAGACGAGG ATGAGAGGGA GGAGAAGAGA 
CGAAAAACGA CACGTAAAAA ACTGGAGTCA CACACCGTTC CCGTTCAGAG CAGATCGGAG 
AGAAAAGCCG CTCAAAGCAA AAGTGTGATC CCGAAGATCA ACTCACCAAA GTGCCCCGAG 
TGTGGCCAGC ACCTAGACGA CCCTAACCTG AAGTACCAGC AGCACCCTGA GGATGCTGTG 
GATGAACCCC AGATGTTGAC CAGTGAGAAA CTGTCCATCT ACGACTCCAC CTCGACCTGG 
TTTGATACTT ATGAAGATTC TCCCATGCAT AGGTTCACTT CCTTCAGTGT GTACTGCAGT 
CGCGGGCACC TGTGTCCTGT CGACACCGGT CTCATTGAGA AGAATGTAGA GCTCTACTTT 
TCTGGGTGTG CCAAAGCAAT TCATGACGAG AATCCATCTA TGGAAGGTGG TATTAATGGC 
AAAAACCTCG GGCCAATCAA TCAGTGGTGG CTCAGTGGCT TTGATGGTGG CGAGAAGGTG 
CTCATTGGCT TCTCCACTGC ATTTGCTGAA TACATTTTGA TGGAGCCCAG CAAAGAGTAT 
GAGCCAATAT TTGGGCTGAT GCAGGAGAAA ATTTACATCA GCAAGATTGT TGTTGAGTTC 
CTGCAAAACA ATCCTGATGC TGTATATGAA GACCTGATCA ATAAGATTGA GACCACTGTT 
CCTCCTTCTA CCATTAATGT GAACCGGTTC ACAGAGGACT CCCTCTTACG CCACGCCCAG 
TTTGTAGTGA GCCAGGTAGA GAGTTACGAC GAAGCCAAGG ACGATGATGA GACCCCCATC 
TTCTTGTCTC CCTGTATGAG AGCCCTGATC CATTTGGCTG GTGTCTCCCT GGGACAGAGG 
CGAGCAACAA GGCGCGTCAT GGGTGCTACC AAGGAGAAGG ACAAAGCACC CACGAAAGCC 
ACCACCACCA AGCTGGTCTA TCAGATCTTT GACACTTTCT TCTCAGAGCA GATTGAGAAG 
TATGATAAGG AGGACAAGGA GAATGCCATG AAGCGCCGCC GCTGTGGTGT CTGTGAGGTC 
TGTCAGCAGC CTGAGTGTGG GAAGTGCAAG GCGTGCAAAG ATATGGTGAA GTTTGGTGGC 
ACTGGACGGA GTAAGCAGGC TTGCCTCAAG AGGAGGTGTC CTAACTTGGC GGTGAAGGAG 
GCAGACGACG ATGAAGAGGC TGATGATGAT GTGTCAGAGA TGCCATCACC CAAAAAGCTG 
CATCAGGGGA AGAAGAAGAA GCAGAACAAG GACCGCATCT CCTGGCTTGG GCAGCCTATG 
AAGATTGAAG AGAATAGAAC TTACTATCAG AAGGTGAGCA TCGATGAGGA GATGCTAGAG 
GTGGGCGACT GCGTCTCGGT CATTCCAGAT GATTCCTCCA AACCACTCTA TCTAGCCAGG 
GTCACAGCTC TGTGGGAAGA CAAAAATGGT CAGATGATGT TCCATGCGCA CTGGTTCTGC 
GCTGGGACAG ACACAGTCCT GGGAGCCACC TCCGACCCCC TGGAACTGTT CCTGGTGGGC 
GAGTGCGAAA ACATGCAGCT TTCCTACATC CACAGCAAGG TCAAGGTCAT CTACAAAGCC 
CCTTCTGAAA ACTGGGCCAT GGAGGGAGGC ACAGACCCTG AGACCACACT GCCTGGGGCT 
GAGGATGGCA AGACTTACTT CTTCCAGCTC TGGTACAACC AGGAGTACGC AAGGTTTGAA 
TCCCCACCCA AGACCCAGCC GACCGAGGAC AACAAGCACA AGTTCTGCCT ATCTTGTATC 
CGGCTGGCTG AGCTGAGACA AAAAGAAATG CCCAAGGTCC TGGAACAAAT TGAGGAGGTG 
GATGGCCGGG TCTACTGCAG TTCCATCACC AAGAATGGTG TTGTCTACCG ACTGGGTGAC 
AGTGTGTACC TTCCTCCCGA GGCCTTTACT TTCAACATCA AAGTGGCTAG CCCCGTGAAA 
CGCCCAAAGA AGGATCCTGT GAACGAGACC CTGTACCCTG AGCACTACCG CAAGTATTCT 
GACTACATCA AGGGGAGCAA CCTGGATGCT CCAGAGCCCT ATCGCATCGG TCGGATAAAA 
GAGATCCACT GTGGCAAGAA GAAAGGCAAG GTCAACGAGG CAGACATCAA GCTGAGGCTC 
TACAAGTTCT ACAGGCCTGA GAATACCCAC AGGTCCTACA ACGGATCCTA TCACACTGAC 
ATCAACATGC TTTACTGGAG CGACGAGGAA GCTGTGGTGA ACTTCAGCGA CGTGCAGGGC 
CGCTGTACCG TGGAGTACGG GGAAGACCTA CTTGAGAGCA TCCAGGATTA TTCACAAGGG 
GGCCCTGACC GCTTCTACTT CCTCGAGGCC TACAATTCAA AGACCAAGAA CTTTGAAGAC 
CCACCAAACC ATGCCCGCAG CCCTGGGAAC AAAGGGAAAG GGAAGGGGAA AGGGAAGGGG 
AAGGGGAAGC ATCAGGTGTC AGAGCCCAAA GAGCCTGAGG CAGCCATCAA ACTGCCCAAG 
CTCCGGACCC TGGATGTGTT TTCCGGCTGT GGAGGGTTAT CGGAAGGATT CCACCAAGCA 
GGCATCTCGG AAACGCTGTG GGCCATCGAG ATGTGGGACC CGGCAGCCCA GGCATTTCGG 
CTGAACAACC CCGGCACCAC AGTGTTCACA GAGGACTGCA ACGTGCTTCT TAAGCTGGTC 
ATGGCTGGGG AGGTGACCAA CTCTCTGGGC CAAAGGCTGC CACAGAAGGG CGATGTGGAG 
ATGCTGTGTG GTGGGCCACC CTGCCAGGGC TTCAGTGGCA TGAACCGCTT CAACTCCCGC 
ACTTACTCCA AGTTCAAAAA CTCCCTAGTG GTCTCCTTCC TCAGCTACTG TGACTACTAC 
CGGCCTCGGT TCTTCCTTCT GGAGAACGTC AGGAACTTCG TGTCCTACAG ACGCTCCATG 
GTGCTGAAGC TCACACTGCG CTGCCTGGTC CGCATGGGCT ACCAGTGCAC CTTTGGTGTG 
CTCCAGGCTG GACAGTATGG CGTGGCCCAG ACACGAAGGA GGGCCATCAT CTTGGCTGCA 
GCCCCAGGAG AAAAGCTGCC TCTGTTCCCA GAGCCTCTGC ATGTGTTTGC GCCCCGTGCC 
TGCCAGCTGA GCGTTGTGGT GGATGACAAG AAGTTTGTTA GCAACATAAC GAGGCTGAGC 
TCGGGGCCCT TCCGAACCAT CACCGTGCGA GACACCATGT CTGACCTCCC CGAGATCCAG 
AATGGAGCCT CGAATTCTGA GATCCCCTAC AATGGAGAGC CACTGTCCTG GTTCCAGAGG 
CAGCTGCGAG GATCACACTA CCAGCCCATC CTCAGGGACC ATATCTGCAA GGACATGAGC 
CCACTGGTGG CTGCCCGCAT GCGGCACATC CCACTGTTCC CAGGATCAGA TTGGCGTGAC 
CTGCCCAACA TACAGGTGCG GCTGGGAGAT GGCGTCATAG CCCATAAGCT ACAGTACACC 
TTTCATGATG TGAAAAATGG CTACAGCAGC ACCGGTGCCC TGCGTGGAGT CTGTTCCTGT 
GCAGAAGGCA AGGCCTGCGA CCCTGAGTCC AGGCAATTCA GCACCCTCAT CCCCTGGTGC 
CTGCCGCACA CTGGGAACCG GCACAACCAC TGGGCTGGCC TCTACGGGCG TCTGGAGTGG 
GATGGCTTCT TCAGCACCAC TGTCACCAAC CCTGAGCCCA TGGGCAAGCA GGGTCGGGTG 
CTCCACCCGG AGCAGCACCG GGTCGTGAGT GTTCGGGAAT GTGCCCGCTC CCAGGGCTTT 
CCAGATAGCT ACCGGTTCTT CGGCAACATC CTGGACAGAC ACCGGCAGGT GGGTAATGCT 
GTGCCACCAC CCCTGGCCAA AGCCATTGGC CTGGAGATTA AGCTCTGCCT GCTGTCCAGT 
GCTCGGGAGA GCGCATCAGC TGCAGTTAAA GCAAAAGAGG AGGCTGCTAC CAAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of DNMT1-DNA complex reveals a role for autoinhibition in maintenance DNA methylation.
Science (New York, N.Y.)331(6020)1036-40(2011 Feb)
Song J,Rechkoblit O,Bestor TH,Patel DJ

Biological functions of DNA methyltransferase 1 require its methyltransferase activity.
Molecular and cellular biology27(11)3891-9(2007 Jun)
Damelin M,Bestor TH

Stage-by-stage change in DNA methylation status of Dnmt1 locus during mouse early development.
The Journal of biological chemistry280(10)9627-34(2005 Mar)
Ko YG,Nishino K,Hattori N,Arai Y,Tanaka S,Shiota K

The activity of the murine DNA methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA.
Journal of molecular biology309(5)1189-99(2001 Jun)
Fatemi M,Hermann A,Pradhan S,Jeltsch A

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Dnmt1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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