DNMT1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001318730.2	NP_001305659.1	DNA (cytosine-5)-methyltransferase 1 isoform c
NM_001379.3	NP_001370.1	DNA (cytosine-5)-methyltransferase 1 isoform b
NM_001130823.2	NP_001124295.1	DNA (cytosine-5)-methyltransferase 1 isoform a
NM_001130823.3	NP_001124295.1	DNA (cytosine-5)-methyltransferase 1 isoform a
NM_001130823.1	NP_001124295.1	DNA (cytosine-5)-methyltransferase 1 isoform a
NM_001318731.1	NP_001305660.1	DNA (cytosine-5)-methyltransferase 1 isoform d
NM_001318731.2	NP_001305660.1	DNA (cytosine-5)-methyltransferase 1 isoform d
NM_001318730.1	NP_001305659.1	DNA (cytosine-5)-methyltransferase 1 isoform c
NM_001379.4	NP_001370.1	DNA (cytosine-5)-methyltransferase 1 isoform b

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27021	NM_001130823.3 Latest version!	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu102073	NM_001318731.2 Latest version!	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$811.30 $1159.00
OHu26697	NM_001379.4 Latest version!	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu57334	NM_001318730.2 Latest version!	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OHu27021	NM_001130823.2	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu27021	NM_001130823.1	Homo sapiens DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu102073	NM_001318731.1	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$811.30 $1159.00
OHu57334	NM_001318730.1	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu26697	NM_001379.3	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
Hide Old Accession Versions >>

DNMT1 ( NM_001130823.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124295.1 Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27021
Clone ID Related Accession (Same CDS sequence)	NM_001130823.2 , NM_001130823.3 , NM_001130823.1
Accession Version	NM_001130823.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	DNA (cytosine-5)-methyltransferase 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X63692.1, BC126227.1, BC092517.1, DA653750.1 and HY245803.1. On Jan 9, 2016 this sequence version replaced NM_001130823.1. Transcript Variant: This variant (1, also known as Dnmt1b in PMIDs 10449766 and 10753866) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.96470.1, SRR1803614.160009.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:10721735) ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCGTTCA AGAGACCCTC CTGCCTCAGC CTCCCAAGTA 
ACTGGGATTA GAGCTGAACC TTCACCTAGC CCCAGGATTA CAAGGAAAAG CACCAGGCAA 
ACCACCATCA CATCTCATTT TGCAAAGGGC CCTGCCAAAC GGAAACCTCA GGAAGAGTCT 
GAAAGAGCCA AATCGGATGA GTCCATCAAG GAAGAAGACA AAGACCAGGA TGAGAAGAGA 
CGTAGAGTTA CATCCAGAGA ACGAGTTGCT AGACCGCTTC CTGCAGAAGA ACCTGAAAGA 
GCAAAATCAG GAACGCGCAC TGAAAAGGAA GAAGAAAGAG ATGAAAAAGA AGAAAAGAGA 
CTCCGAAGTC AAACCAAAGA ACCAACACCC AAACAGAAAC TGAAGGAGGA GCCGGACAGA 
GAAGCCAGGG CAGGCGTGCA GGCTGACGAG GACGAAGATG GAGACGAGAA AGATGAGAAG 
AAGCACAGAA GTCAACCCAA AGATCTAGCT GCCAAACGGA GGCCCGAAGA AAAAGAACCT 
GAAAAAGTAA ATCCACAGAT TTCTGATGAA AAAGACGAGG ATGAAAAGGA GGAGAAGAGA 
CGCAAAACGA CCCCCAAAGA ACCAACGGAG AAAAAAATGG CTCGCGCCAA AACAGTCATG 
AACTCCAAGA CCCACCCTCC CAAGTGCATT CAGTGCGGGC AGTACCTGGA CGACCCTGAC 
CTCAAATATG GGCAGCACCC ACCAGACGCG GTGGATGAGC CACAGATGCT GACAAATGAG 
AAGCTGTCCA TCTTTGATGC CAACGAGTCT GGCTTTGAGA GTTATGAGGC GCTTCCCCAG 
CACAAACTGA CCTGCTTCAG TGTGTACTGT AAGCACGGTC ACCTGTGTCC CATCGACACC 
GGCCTCATCG AGAAGAATAT CGAACTCTTC TTTTCTGGTT CAGCAAAACC AATCTATGAT 
GATGACCCAT CTCTTGAAGG TGGTGTTAAT GGCAAAAATC TTGGCCCCAT AAATGAATGG 
TGGATCACTG GCTTTGATGG AGGTGAAAAG GCCCTCATCG GCTTCAGCAC CTCATTTGCC 
GAATACATTC TGATGGATCC CAGTCCCGAG TATGCGCCCA TATTTGGGCT GATGCAGGAG 
AAGATCTACA TCAGCAAGAT TGTGGTGGAG TTCCTGCAGA GCAATTCCGA CTCGACCTAT 
GAGGACCTGA TCAACAAGAT CGAGACCACG GTTCCTCCTT CTGGCCTCAA CTTGAACCGC 
TTCACAGAGG ACTCCCTCCT GCGACACGCG CAGTTTGTGG TGGAGCAGGT GGAGAGTTAT 
GACGAGGCCG GGGACAGTGA TGAGCAGCCC ATCTTCCTGA CACCCTGCAT GCGGGACCTG 
ATCAAGCTGG CTGGGGTCAC GCTGGGACAG AGGCGAGCCC AGGCGAGGCG GCAGACCATC 
AGGCATTCTA CCAGGGAGAA GGACAGGGGA CCCACGAAAG CCACCACCAC CAAGCTGGTC 
TACCAGATCT TCGATACTTT CTTCGCAGAG CAAATTGAAA AGGATGACAG AGAAGACAAG 
GAGAACGCCT TTAAGCGCCG GCGATGTGGC GTCTGTGAGG TGTGTCAGCA GCCTGAGTGT 
GGGAAATGTA AAGCCTGCAA GGACATGGTT AAATTTGGTG GCAGTGGACG GAGCAAGCAG 
GCTTGCCAAG AGCGGAGGTG TCCCAATATG GCCATGAAGG AGGCAGATGA CGATGAGGAA 
GTCGATGATA ACATCCCAGA GATGCCGTCA CCCAAAAAAA TGCACCAGGG GAAGAAGAAG 
AAACAGAACA AGAATCGCAT CTCTTGGGTC GGAGAAGCCG TCAAGACTGA TGGGAAGAAG 
AGTTACTATA AGAAGGTGTG CATTGATGCG GAAACCCTGG AAGTGGGGGA CTGTGTCTCT 
GTTATTCCAG ATGATTCCTC AAAACCGCTG TATCTAGCAA GGGTCACGGC GCTGTGGGAG 
GACAGCAGCA ACGGGCAGAT GTTTCACGCC CACTGGTTCT GCGCTGGGAC AGACACAGTC 
CTCGGGGCCA CGTCGGACCC TCTGGAGCTG TTCTTGGTGG ATGAATGTGA GGACATGCAG 
CTTTCATATA TCCACAGCAA AGTGAAAGTC ATCTACAAAG CCCCCTCCGA AAACTGGGCC 
ATGGAGGGAG GCATGGATCC CGAGTCCCTG CTGGAGGGGG ACGACGGGAA GACCTACTTC 
TACCAGCTGT GGTATGATCA AGACTACGCG AGATTCGAGT CCCCTCCAAA AACCCAGCCA 
ACAGAGGACA ACAAGTTCAA ATTCTGTGTG AGCTGTGCCC GTCTGGCTGA GATGAGGCAA 
AAAGAAATCC CCAGGGTCCT GGAGCAGCTC GAGGACCTGG ATAGCCGGGT CCTCTACTAC 
TCAGCCACCA AGAACGGCAT CCTGTACCGA GTTGGTGATG GTGTGTACCT GCCCCCTGAG 
GCCTTCACGT TCAACATCAA GCTGTCCAGT CCCGTGAAAC GCCCACGGAA GGAGCCCGTG 
GATGAGGACC TGTACCCAGA GCACTACCGG AAATACTCCG ACTACATCAA AGGCAGCAAC 
CTGGATGCCC CTGAGCCCTA CCGAATTGGC CGGATCAAAG AGATCTTCTG TCCCAAGAAG 
AGCAACGGCA GGCCCAATGA GACTGACATC AAAATCCGGG TCAACAAGTT CTACAGGCCT 
GAGAACACCC ACAAGTCCAC TCCAGCGAGC TACCACGCAG ACATCAACCT GCTCTACTGG 
AGCGACGAGG AGGCCGTGGT GGACTTCAAG GCTGTGCAGG GCCGCTGCAC CGTGGAGTAT 
GGGGAGGACC TGCCCGAGTG CGTCCAGGTG TACTCCATGG GCGGCCCCAA CCGCTTCTAC 
TTCCTCGAGG CCTATAATGC AAAGAGCAAA AGCTTTGAAG ATCCTCCCAA CCATGCCCGT 
AGCCCTGGAA ACAAAGGGAA GGGCAAGGGA AAAGGGAAGG GCAAGCCCAA GTCCCAAGCC 
TGTGAGCCGA GCGAGCCAGA GATAGAGATC AAGCTGCCCA AGCTGCGGAC CCTGGATGTG 
TTTTCTGGCT GCGGGGGGTT GTCGGAGGGA TTCCACCAAG CAGGCATCTC TGACACGCTG 
TGGGCCATCG AGATGTGGGA CCCTGCGGCC CAGGCGTTCC GGCTGAACAA CCCCGGCTCC 
ACAGTGTTCA CAGAGGACTG CAACATCCTG CTGAAGCTGG TCATGGCTGG GGAGACCACC 
AACTCCCGCG GCCAGCGGCT GCCCCAGAAG GGAGACGTGG AGATGCTGTG CGGCGGGCCG 
CCCTGCCAGG GCTTCAGCGG CATGAACCGC TTCAATTCGC GCACCTACTC CAAGTTCAAA 
AACTCTCTGG TGGTTTCCTT CCTCAGCTAC TGCGACTACT ACCGGCCCCG GTTCTTCCTC 
CTGGAGAATG TCAGGAACTT TGTCTCCTTC AAGCGCTCCA TGGTCCTGAA GCTCACCCTC 
CGCTGCCTGG TCCGCATGGG CTATCAGTGC ACCTTCGGCG TGCTGCAGGC CGGTCAGTAC 
GGCGTGGCCC AGACTAGGAG GCGGGCCATC ATCCTGGCCG CGGCCCCTGG AGAGAAGCTC 
CCTCTGTTCC CGGAGCCACT GCACGTGTTT GCTCCCCGGG CCTGCCAGCT GAGCGTGGTG 
GTGGATGACA AGAAGTTTGT GAGCAACATA ACCAGGTTGA GCTCGGGTCC TTTCCGGACC 
ATCACGGTGC GAGACACGAT GTCCGACCTG CCGGAGGTGC GGAATGGAGC CTCGGCACTG 
GAGATCTCCT ACAACGGGGA GCCTCAGTCC TGGTTCCAGA GGCAGCTCCG GGGCGCACAG 
TACCAGCCCA TCCTCAGGGA CCACATCTGT AAGGACATGA GTGCATTGGT GGCTGCCCGC 
ATGCGGCACA TCCCCTTGGC CCCAGGGTCA GACTGGCGCG ATCTGCCCAA CATCGAGGTG 
CGGCTCTCAG ACGGCACCAT GGCCAGGAAG CTGCGGTATA CCCACCATGA CAGGAAGAAC 
GGCCGCAGCA GCTCTGGGGC CCTCCGTGGG GTCTGCTCCT GCGTGGAAGC CGGCAAAGCC 
TGCGACCCCG CAGCCAGGCA GTTCAACACC CTCATCCCCT GGTGCCTGCC CCACACCGGG 
AACCGGCACA ACCACTGGGC TGGCCTCTAT GGAAGGCTCG AGTGGGACGG CTTCTTCAGC 
ACAACCGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCAGAGCAG 
CACCGTGTGG TGAGCGTGCG GGAGTGTGCC CGCTCCCAGG GCTTCCCTGA CACCTACCGG 
CTCTTCGGCA ACATCCTGGA CAAGCACCGG CAGGTGGGCA ATGCCGTGCC ACCGCCCCTG 
GCCAAAGCCA TTGGCTTGGA GATCAAGCTT TGTATGTTGG CCAAAGCCCG AGAGAGTGCC 
TCAGCTAAAA TAAAGGAGGA GGAAGCTGCT AAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124295.1
CDS	237..5135
Translation	MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKRSRDPPASASQVTGIRAEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

Target ORF information:

RefSeq Version	NM_001130823.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130823.2

ORF Insert Sequence:

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCGTTCA AGAGACCCTC CTGCCTCAGC CTCCCAAGTA 
ACTGGGATTA GAGCTGAACC TTCACCTAGC CCCAGGATTA CAAGGAAAAG CACCAGGCAA 
ACCACCATCA CATCTCATTT TGCAAAGGGC CCTGCCAAAC GGAAACCTCA GGAAGAGTCT 
GAAAGAGCCA AATCGGATGA GTCCATCAAG GAAGAAGACA AAGACCAGGA TGAGAAGAGA 
CGTAGAGTTA CATCCAGAGA ACGAGTTGCT AGACCGCTTC CTGCAGAAGA ACCTGAAAGA 
GCAAAATCAG GAACGCGCAC TGAAAAGGAA GAAGAAAGAG ATGAAAAAGA AGAAAAGAGA 
CTCCGAAGTC AAACCAAAGA ACCAACACCC AAACAGAAAC TGAAGGAGGA GCCGGACAGA 
GAAGCCAGGG CAGGCGTGCA GGCTGACGAG GACGAAGATG GAGACGAGAA AGATGAGAAG 
AAGCACAGAA GTCAACCCAA AGATCTAGCT GCCAAACGGA GGCCCGAAGA AAAAGAACCT 
GAAAAAGTAA ATCCACAGAT TTCTGATGAA AAAGACGAGG ATGAAAAGGA GGAGAAGAGA 
CGCAAAACGA CCCCCAAAGA ACCAACGGAG AAAAAAATGG CTCGCGCCAA AACAGTCATG 
AACTCCAAGA CCCACCCTCC CAAGTGCATT CAGTGCGGGC AGTACCTGGA CGACCCTGAC 
CTCAAATATG GGCAGCACCC ACCAGACGCG GTGGATGAGC CACAGATGCT GACAAATGAG 
AAGCTGTCCA TCTTTGATGC CAACGAGTCT GGCTTTGAGA GTTATGAGGC GCTTCCCCAG 
CACAAACTGA CCTGCTTCAG TGTGTACTGT AAGCACGGTC ACCTGTGTCC CATCGACACC 
GGCCTCATCG AGAAGAATAT CGAACTCTTC TTTTCTGGTT CAGCAAAACC AATCTATGAT 
GATGACCCAT CTCTTGAAGG TGGTGTTAAT GGCAAAAATC TTGGCCCCAT AAATGAATGG 
TGGATCACTG GCTTTGATGG AGGTGAAAAG GCCCTCATCG GCTTCAGCAC CTCATTTGCC 
GAATACATTC TGATGGATCC CAGTCCCGAG TATGCGCCCA TATTTGGGCT GATGCAGGAG 
AAGATCTACA TCAGCAAGAT TGTGGTGGAG TTCCTGCAGA GCAATTCCGA CTCGACCTAT 
GAGGACCTGA TCAACAAGAT CGAGACCACG GTTCCTCCTT CTGGCCTCAA CTTGAACCGC 
TTCACAGAGG ACTCCCTCCT GCGACACGCG CAGTTTGTGG TGGAGCAGGT GGAGAGTTAT 
GACGAGGCCG GGGACAGTGA TGAGCAGCCC ATCTTCCTGA CACCCTGCAT GCGGGACCTG 
ATCAAGCTGG CTGGGGTCAC GCTGGGACAG AGGCGAGCCC AGGCGAGGCG GCAGACCATC 
AGGCATTCTA CCAGGGAGAA GGACAGGGGA CCCACGAAAG CCACCACCAC CAAGCTGGTC 
TACCAGATCT TCGATACTTT CTTCGCAGAG CAAATTGAAA AGGATGACAG AGAAGACAAG 
GAGAACGCCT TTAAGCGCCG GCGATGTGGC GTCTGTGAGG TGTGTCAGCA GCCTGAGTGT 
GGGAAATGTA AAGCCTGCAA GGACATGGTT AAATTTGGTG GCAGTGGACG GAGCAAGCAG 
GCTTGCCAAG AGCGGAGGTG TCCCAATATG GCCATGAAGG AGGCAGATGA CGATGAGGAA 
GTCGATGATA ACATCCCAGA GATGCCGTCA CCCAAAAAAA TGCACCAGGG GAAGAAGAAG 
AAACAGAACA AGAATCGCAT CTCTTGGGTC GGAGAAGCCG TCAAGACTGA TGGGAAGAAG 
AGTTACTATA AGAAGGTGTG CATTGATGCG GAAACCCTGG AAGTGGGGGA CTGTGTCTCT 
GTTATTCCAG ATGATTCCTC AAAACCGCTG TATCTAGCAA GGGTCACGGC GCTGTGGGAG 
GACAGCAGCA ACGGGCAGAT GTTTCACGCC CACTGGTTCT GCGCTGGGAC AGACACAGTC 
CTCGGGGCCA CGTCGGACCC TCTGGAGCTG TTCTTGGTGG ATGAATGTGA GGACATGCAG 
CTTTCATATA TCCACAGCAA AGTGAAAGTC ATCTACAAAG CCCCCTCCGA AAACTGGGCC 
ATGGAGGGAG GCATGGATCC CGAGTCCCTG CTGGAGGGGG ACGACGGGAA GACCTACTTC 
TACCAGCTGT GGTATGATCA AGACTACGCG AGATTCGAGT CCCCTCCAAA AACCCAGCCA 
ACAGAGGACA ACAAGTTCAA ATTCTGTGTG AGCTGTGCCC GTCTGGCTGA GATGAGGCAA 
AAAGAAATCC CCAGGGTCCT GGAGCAGCTC GAGGACCTGG ATAGCCGGGT CCTCTACTAC 
TCAGCCACCA AGAACGGCAT CCTGTACCGA GTTGGTGATG GTGTGTACCT GCCCCCTGAG 
GCCTTCACGT TCAACATCAA GCTGTCCAGT CCCGTGAAAC GCCCACGGAA GGAGCCCGTG 
GATGAGGACC TGTACCCAGA GCACTACCGG AAATACTCCG ACTACATCAA AGGCAGCAAC 
CTGGATGCCC CTGAGCCCTA CCGAATTGGC CGGATCAAAG AGATCTTCTG TCCCAAGAAG 
AGCAACGGCA GGCCCAATGA GACTGACATC AAAATCCGGG TCAACAAGTT CTACAGGCCT 
GAGAACACCC ACAAGTCCAC TCCAGCGAGC TACCACGCAG ACATCAACCT GCTCTACTGG 
AGCGACGAGG AGGCCGTGGT GGACTTCAAG GCTGTGCAGG GCCGCTGCAC CGTGGAGTAT 
GGGGAGGACC TGCCCGAGTG CGTCCAGGTG TACTCCATGG GCGGCCCCAA CCGCTTCTAC 
TTCCTCGAGG CCTATAATGC AAAGAGCAAA AGCTTTGAAG ATCCTCCCAA CCATGCCCGT 
AGCCCTGGAA ACAAAGGGAA GGGCAAGGGA AAAGGGAAGG GCAAGCCCAA GTCCCAAGCC 
TGTGAGCCGA GCGAGCCAGA GATAGAGATC AAGCTGCCCA AGCTGCGGAC CCTGGATGTG 
TTTTCTGGCT GCGGGGGGTT GTCGGAGGGA TTCCACCAAG CAGGCATCTC TGACACGCTG 
TGGGCCATCG AGATGTGGGA CCCTGCGGCC CAGGCGTTCC GGCTGAACAA CCCCGGCTCC 
ACAGTGTTCA CAGAGGACTG CAACATCCTG CTGAAGCTGG TCATGGCTGG GGAGACCACC 
AACTCCCGCG GCCAGCGGCT GCCCCAGAAG GGAGACGTGG AGATGCTGTG CGGCGGGCCG 
CCCTGCCAGG GCTTCAGCGG CATGAACCGC TTCAATTCGC GCACCTACTC CAAGTTCAAA 
AACTCTCTGG TGGTTTCCTT CCTCAGCTAC TGCGACTACT ACCGGCCCCG GTTCTTCCTC 
CTGGAGAATG TCAGGAACTT TGTCTCCTTC AAGCGCTCCA TGGTCCTGAA GCTCACCCTC 
CGCTGCCTGG TCCGCATGGG CTATCAGTGC ACCTTCGGCG TGCTGCAGGC CGGTCAGTAC 
GGCGTGGCCC AGACTAGGAG GCGGGCCATC ATCCTGGCCG CGGCCCCTGG AGAGAAGCTC 
CCTCTGTTCC CGGAGCCACT GCACGTGTTT GCTCCCCGGG CCTGCCAGCT GAGCGTGGTG 
GTGGATGACA AGAAGTTTGT GAGCAACATA ACCAGGTTGA GCTCGGGTCC TTTCCGGACC 
ATCACGGTGC GAGACACGAT GTCCGACCTG CCGGAGGTGC GGAATGGAGC CTCGGCACTG 
GAGATCTCCT ACAACGGGGA GCCTCAGTCC TGGTTCCAGA GGCAGCTCCG GGGCGCACAG 
TACCAGCCCA TCCTCAGGGA CCACATCTGT AAGGACATGA GTGCATTGGT GGCTGCCCGC 
ATGCGGCACA TCCCCTTGGC CCCAGGGTCA GACTGGCGCG ATCTGCCCAA CATCGAGGTG 
CGGCTCTCAG ACGGCACCAT GGCCAGGAAG CTGCGGTATA CCCACCATGA CAGGAAGAAC 
GGCCGCAGCA GCTCTGGGGC CCTCCGTGGG GTCTGCTCCT GCGTGGAAGC CGGCAAAGCC 
TGCGACCCCG CAGCCAGGCA GTTCAACACC CTCATCCCCT GGTGCCTGCC CCACACCGGG 
AACCGGCACA ACCACTGGGC TGGCCTCTAT GGAAGGCTCG AGTGGGACGG CTTCTTCAGC 
ACAACCGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCAGAGCAG 
CACCGTGTGG TGAGCGTGCG GGAGTGTGCC CGCTCCCAGG GCTTCCCTGA CACCTACCGG 
CTCTTCGGCA ACATCCTGGA CAAGCACCGG CAGGTGGGCA ATGCCGTGCC ACCGCCCCTG 
GCCAAAGCCA TTGGCTTGGA GATCAAGCTT TGTATGTTGG CCAAAGCCCG AGAGAGTGCC 
TCAGCTAAAA TAAAGGAGGA GGAAGCTGCT AAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNMT1 ( NM_001130823.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124295.1 Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27021
Clone ID Related Accession (Same CDS sequence)	NM_001130823.2 , NM_001130823.3 , NM_001130823.1
Accession Version	NM_001130823.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	DNA (cytosine-5)-methyltransferase 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC126227.1, BC092517.1, DA653750.1 and X63692.1. On Nov 24, 2018 this sequence version replaced NM_001130823.2. Transcript Variant: This variant (1, also known as Dnmt1b in PMIDs 10449766 and 10753866) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.96470.1, SRR1803614.160009.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000359526.9/ ENSP00000352516.3 RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCGTTCA AGAGACCCTC CTGCCTCAGC CTCCCAAGTA 
ACTGGGATTA GAGCTGAACC TTCACCTAGC CCCAGGATTA CAAGGAAAAG CACCAGGCAA 
ACCACCATCA CATCTCATTT TGCAAAGGGC CCTGCCAAAC GGAAACCTCA GGAAGAGTCT 
GAAAGAGCCA AATCGGATGA GTCCATCAAG GAAGAAGACA AAGACCAGGA TGAGAAGAGA 
CGTAGAGTTA CATCCAGAGA ACGAGTTGCT AGACCGCTTC CTGCAGAAGA ACCTGAAAGA 
GCAAAATCAG GAACGCGCAC TGAAAAGGAA GAAGAAAGAG ATGAAAAAGA AGAAAAGAGA 
CTCCGAAGTC AAACCAAAGA ACCAACACCC AAACAGAAAC TGAAGGAGGA GCCGGACAGA 
GAAGCCAGGG CAGGCGTGCA GGCTGACGAG GACGAAGATG GAGACGAGAA AGATGAGAAG 
AAGCACAGAA GTCAACCCAA AGATCTAGCT GCCAAACGGA GGCCCGAAGA AAAAGAACCT 
GAAAAAGTAA ATCCACAGAT TTCTGATGAA AAAGACGAGG ATGAAAAGGA GGAGAAGAGA 
CGCAAAACGA CCCCCAAAGA ACCAACGGAG AAAAAAATGG CTCGCGCCAA AACAGTCATG 
AACTCCAAGA CCCACCCTCC CAAGTGCATT CAGTGCGGGC AGTACCTGGA CGACCCTGAC 
CTCAAATATG GGCAGCACCC ACCAGACGCG GTGGATGAGC CACAGATGCT GACAAATGAG 
AAGCTGTCCA TCTTTGATGC CAACGAGTCT GGCTTTGAGA GTTATGAGGC GCTTCCCCAG 
CACAAACTGA CCTGCTTCAG TGTGTACTGT AAGCACGGTC ACCTGTGTCC CATCGACACC 
GGCCTCATCG AGAAGAATAT CGAACTCTTC TTTTCTGGTT CAGCAAAACC AATCTATGAT 
GATGACCCAT CTCTTGAAGG TGGTGTTAAT GGCAAAAATC TTGGCCCCAT AAATGAATGG 
TGGATCACTG GCTTTGATGG AGGTGAAAAG GCCCTCATCG GCTTCAGCAC CTCATTTGCC 
GAATACATTC TGATGGATCC CAGTCCCGAG TATGCGCCCA TATTTGGGCT GATGCAGGAG 
AAGATCTACA TCAGCAAGAT TGTGGTGGAG TTCCTGCAGA GCAATTCCGA CTCGACCTAT 
GAGGACCTGA TCAACAAGAT CGAGACCACG GTTCCTCCTT CTGGCCTCAA CTTGAACCGC 
TTCACAGAGG ACTCCCTCCT GCGACACGCG CAGTTTGTGG TGGAGCAGGT GGAGAGTTAT 
GACGAGGCCG GGGACAGTGA TGAGCAGCCC ATCTTCCTGA CACCCTGCAT GCGGGACCTG 
ATCAAGCTGG CTGGGGTCAC GCTGGGACAG AGGCGAGCCC AGGCGAGGCG GCAGACCATC 
AGGCATTCTA CCAGGGAGAA GGACAGGGGA CCCACGAAAG CCACCACCAC CAAGCTGGTC 
TACCAGATCT TCGATACTTT CTTCGCAGAG CAAATTGAAA AGGATGACAG AGAAGACAAG 
GAGAACGCCT TTAAGCGCCG GCGATGTGGC GTCTGTGAGG TGTGTCAGCA GCCTGAGTGT 
GGGAAATGTA AAGCCTGCAA GGACATGGTT AAATTTGGTG GCAGTGGACG GAGCAAGCAG 
GCTTGCCAAG AGCGGAGGTG TCCCAATATG GCCATGAAGG AGGCAGATGA CGATGAGGAA 
GTCGATGATA ACATCCCAGA GATGCCGTCA CCCAAAAAAA TGCACCAGGG GAAGAAGAAG 
AAACAGAACA AGAATCGCAT CTCTTGGGTC GGAGAAGCCG TCAAGACTGA TGGGAAGAAG 
AGTTACTATA AGAAGGTGTG CATTGATGCG GAAACCCTGG AAGTGGGGGA CTGTGTCTCT 
GTTATTCCAG ATGATTCCTC AAAACCGCTG TATCTAGCAA GGGTCACGGC GCTGTGGGAG 
GACAGCAGCA ACGGGCAGAT GTTTCACGCC CACTGGTTCT GCGCTGGGAC AGACACAGTC 
CTCGGGGCCA CGTCGGACCC TCTGGAGCTG TTCTTGGTGG ATGAATGTGA GGACATGCAG 
CTTTCATATA TCCACAGCAA AGTGAAAGTC ATCTACAAAG CCCCCTCCGA AAACTGGGCC 
ATGGAGGGAG GCATGGATCC CGAGTCCCTG CTGGAGGGGG ACGACGGGAA GACCTACTTC 
TACCAGCTGT GGTATGATCA AGACTACGCG AGATTCGAGT CCCCTCCAAA AACCCAGCCA 
ACAGAGGACA ACAAGTTCAA ATTCTGTGTG AGCTGTGCCC GTCTGGCTGA GATGAGGCAA 
AAAGAAATCC CCAGGGTCCT GGAGCAGCTC GAGGACCTGG ATAGCCGGGT CCTCTACTAC 
TCAGCCACCA AGAACGGCAT CCTGTACCGA GTTGGTGATG GTGTGTACCT GCCCCCTGAG 
GCCTTCACGT TCAACATCAA GCTGTCCAGT CCCGTGAAAC GCCCACGGAA GGAGCCCGTG 
GATGAGGACC TGTACCCAGA GCACTACCGG AAATACTCCG ACTACATCAA AGGCAGCAAC 
CTGGATGCCC CTGAGCCCTA CCGAATTGGC CGGATCAAAG AGATCTTCTG TCCCAAGAAG 
AGCAACGGCA GGCCCAATGA GACTGACATC AAAATCCGGG TCAACAAGTT CTACAGGCCT 
GAGAACACCC ACAAGTCCAC TCCAGCGAGC TACCACGCAG ACATCAACCT GCTCTACTGG 
AGCGACGAGG AGGCCGTGGT GGACTTCAAG GCTGTGCAGG GCCGCTGCAC CGTGGAGTAT 
GGGGAGGACC TGCCCGAGTG CGTCCAGGTG TACTCCATGG GCGGCCCCAA CCGCTTCTAC 
TTCCTCGAGG CCTATAATGC AAAGAGCAAA AGCTTTGAAG ATCCTCCCAA CCATGCCCGT 
AGCCCTGGAA ACAAAGGGAA GGGCAAGGGA AAAGGGAAGG GCAAGCCCAA GTCCCAAGCC 
TGTGAGCCGA GCGAGCCAGA GATAGAGATC AAGCTGCCCA AGCTGCGGAC CCTGGATGTG 
TTTTCTGGCT GCGGGGGGTT GTCGGAGGGA TTCCACCAAG CAGGCATCTC TGACACGCTG 
TGGGCCATCG AGATGTGGGA CCCTGCGGCC CAGGCGTTCC GGCTGAACAA CCCCGGCTCC 
ACAGTGTTCA CAGAGGACTG CAACATCCTG CTGAAGCTGG TCATGGCTGG GGAGACCACC 
AACTCCCGCG GCCAGCGGCT GCCCCAGAAG GGAGACGTGG AGATGCTGTG CGGCGGGCCG 
CCCTGCCAGG GCTTCAGCGG CATGAACCGC TTCAATTCGC GCACCTACTC CAAGTTCAAA 
AACTCTCTGG TGGTTTCCTT CCTCAGCTAC TGCGACTACT ACCGGCCCCG GTTCTTCCTC 
CTGGAGAATG TCAGGAACTT TGTCTCCTTC AAGCGCTCCA TGGTCCTGAA GCTCACCCTC 
CGCTGCCTGG TCCGCATGGG CTATCAGTGC ACCTTCGGCG TGCTGCAGGC CGGTCAGTAC 
GGCGTGGCCC AGACTAGGAG GCGGGCCATC ATCCTGGCCG CGGCCCCTGG AGAGAAGCTC 
CCTCTGTTCC CGGAGCCACT GCACGTGTTT GCTCCCCGGG CCTGCCAGCT GAGCGTGGTG 
GTGGATGACA AGAAGTTTGT GAGCAACATA ACCAGGTTGA GCTCGGGTCC TTTCCGGACC 
ATCACGGTGC GAGACACGAT GTCCGACCTG CCGGAGGTGC GGAATGGAGC CTCGGCACTG 
GAGATCTCCT ACAACGGGGA GCCTCAGTCC TGGTTCCAGA GGCAGCTCCG GGGCGCACAG 
TACCAGCCCA TCCTCAGGGA CCACATCTGT AAGGACATGA GTGCATTGGT GGCTGCCCGC 
ATGCGGCACA TCCCCTTGGC CCCAGGGTCA GACTGGCGCG ATCTGCCCAA CATCGAGGTG 
CGGCTCTCAG ACGGCACCAT GGCCAGGAAG CTGCGGTATA CCCACCATGA CAGGAAGAAC 
GGCCGCAGCA GCTCTGGGGC CCTCCGTGGG GTCTGCTCCT GCGTGGAAGC CGGCAAAGCC 
TGCGACCCCG CAGCCAGGCA GTTCAACACC CTCATCCCCT GGTGCCTGCC CCACACCGGG 
AACCGGCACA ACCACTGGGC TGGCCTCTAT GGAAGGCTCG AGTGGGACGG CTTCTTCAGC 
ACAACCGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCAGAGCAG 
CACCGTGTGG TGAGCGTGCG GGAGTGTGCC CGCTCCCAGG GCTTCCCTGA CACCTACCGG 
CTCTTCGGCA ACATCCTGGA CAAGCACCGG CAGGTGGGCA ATGCCGTGCC ACCGCCCCTG 
GCCAAAGCCA TTGGCTTGGA GATCAAGCTT TGTATGTTGG CCAAAGCCCG AGAGAGTGCC 
TCAGCTAAAA TAAAGGAGGA GGAAGCTGCT AAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124295.1
CDS	55..4953
Translation	MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKRSRDPPASASQVTGIRAEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

Target ORF information:

RefSeq Version	NM_001130823.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130823.3

ORF Insert Sequence:

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCGTTCA AGAGACCCTC CTGCCTCAGC CTCCCAAGTA 
ACTGGGATTA GAGCTGAACC TTCACCTAGC CCCAGGATTA CAAGGAAAAG CACCAGGCAA 
ACCACCATCA CATCTCATTT TGCAAAGGGC CCTGCCAAAC GGAAACCTCA GGAAGAGTCT 
GAAAGAGCCA AATCGGATGA GTCCATCAAG GAAGAAGACA AAGACCAGGA TGAGAAGAGA 
CGTAGAGTTA CATCCAGAGA ACGAGTTGCT AGACCGCTTC CTGCAGAAGA ACCTGAAAGA 
GCAAAATCAG GAACGCGCAC TGAAAAGGAA GAAGAAAGAG ATGAAAAAGA AGAAAAGAGA 
CTCCGAAGTC AAACCAAAGA ACCAACACCC AAACAGAAAC TGAAGGAGGA GCCGGACAGA 
GAAGCCAGGG CAGGCGTGCA GGCTGACGAG GACGAAGATG GAGACGAGAA AGATGAGAAG 
AAGCACAGAA GTCAACCCAA AGATCTAGCT GCCAAACGGA GGCCCGAAGA AAAAGAACCT 
GAAAAAGTAA ATCCACAGAT TTCTGATGAA AAAGACGAGG ATGAAAAGGA GGAGAAGAGA 
CGCAAAACGA CCCCCAAAGA ACCAACGGAG AAAAAAATGG CTCGCGCCAA AACAGTCATG 
AACTCCAAGA CCCACCCTCC CAAGTGCATT CAGTGCGGGC AGTACCTGGA CGACCCTGAC 
CTCAAATATG GGCAGCACCC ACCAGACGCG GTGGATGAGC CACAGATGCT GACAAATGAG 
AAGCTGTCCA TCTTTGATGC CAACGAGTCT GGCTTTGAGA GTTATGAGGC GCTTCCCCAG 
CACAAACTGA CCTGCTTCAG TGTGTACTGT AAGCACGGTC ACCTGTGTCC CATCGACACC 
GGCCTCATCG AGAAGAATAT CGAACTCTTC TTTTCTGGTT CAGCAAAACC AATCTATGAT 
GATGACCCAT CTCTTGAAGG TGGTGTTAAT GGCAAAAATC TTGGCCCCAT AAATGAATGG 
TGGATCACTG GCTTTGATGG AGGTGAAAAG GCCCTCATCG GCTTCAGCAC CTCATTTGCC 
GAATACATTC TGATGGATCC CAGTCCCGAG TATGCGCCCA TATTTGGGCT GATGCAGGAG 
AAGATCTACA TCAGCAAGAT TGTGGTGGAG TTCCTGCAGA GCAATTCCGA CTCGACCTAT 
GAGGACCTGA TCAACAAGAT CGAGACCACG GTTCCTCCTT CTGGCCTCAA CTTGAACCGC 
TTCACAGAGG ACTCCCTCCT GCGACACGCG CAGTTTGTGG TGGAGCAGGT GGAGAGTTAT 
GACGAGGCCG GGGACAGTGA TGAGCAGCCC ATCTTCCTGA CACCCTGCAT GCGGGACCTG 
ATCAAGCTGG CTGGGGTCAC GCTGGGACAG AGGCGAGCCC AGGCGAGGCG GCAGACCATC 
AGGCATTCTA CCAGGGAGAA GGACAGGGGA CCCACGAAAG CCACCACCAC CAAGCTGGTC 
TACCAGATCT TCGATACTTT CTTCGCAGAG CAAATTGAAA AGGATGACAG AGAAGACAAG 
GAGAACGCCT TTAAGCGCCG GCGATGTGGC GTCTGTGAGG TGTGTCAGCA GCCTGAGTGT 
GGGAAATGTA AAGCCTGCAA GGACATGGTT AAATTTGGTG GCAGTGGACG GAGCAAGCAG 
GCTTGCCAAG AGCGGAGGTG TCCCAATATG GCCATGAAGG AGGCAGATGA CGATGAGGAA 
GTCGATGATA ACATCCCAGA GATGCCGTCA CCCAAAAAAA TGCACCAGGG GAAGAAGAAG 
AAACAGAACA AGAATCGCAT CTCTTGGGTC GGAGAAGCCG TCAAGACTGA TGGGAAGAAG 
AGTTACTATA AGAAGGTGTG CATTGATGCG GAAACCCTGG AAGTGGGGGA CTGTGTCTCT 
GTTATTCCAG ATGATTCCTC AAAACCGCTG TATCTAGCAA GGGTCACGGC GCTGTGGGAG 
GACAGCAGCA ACGGGCAGAT GTTTCACGCC CACTGGTTCT GCGCTGGGAC AGACACAGTC 
CTCGGGGCCA CGTCGGACCC TCTGGAGCTG TTCTTGGTGG ATGAATGTGA GGACATGCAG 
CTTTCATATA TCCACAGCAA AGTGAAAGTC ATCTACAAAG CCCCCTCCGA AAACTGGGCC 
ATGGAGGGAG GCATGGATCC CGAGTCCCTG CTGGAGGGGG ACGACGGGAA GACCTACTTC 
TACCAGCTGT GGTATGATCA AGACTACGCG AGATTCGAGT CCCCTCCAAA AACCCAGCCA 
ACAGAGGACA ACAAGTTCAA ATTCTGTGTG AGCTGTGCCC GTCTGGCTGA GATGAGGCAA 
AAAGAAATCC CCAGGGTCCT GGAGCAGCTC GAGGACCTGG ATAGCCGGGT CCTCTACTAC 
TCAGCCACCA AGAACGGCAT CCTGTACCGA GTTGGTGATG GTGTGTACCT GCCCCCTGAG 
GCCTTCACGT TCAACATCAA GCTGTCCAGT CCCGTGAAAC GCCCACGGAA GGAGCCCGTG 
GATGAGGACC TGTACCCAGA GCACTACCGG AAATACTCCG ACTACATCAA AGGCAGCAAC 
CTGGATGCCC CTGAGCCCTA CCGAATTGGC CGGATCAAAG AGATCTTCTG TCCCAAGAAG 
AGCAACGGCA GGCCCAATGA GACTGACATC AAAATCCGGG TCAACAAGTT CTACAGGCCT 
GAGAACACCC ACAAGTCCAC TCCAGCGAGC TACCACGCAG ACATCAACCT GCTCTACTGG 
AGCGACGAGG AGGCCGTGGT GGACTTCAAG GCTGTGCAGG GCCGCTGCAC CGTGGAGTAT 
GGGGAGGACC TGCCCGAGTG CGTCCAGGTG TACTCCATGG GCGGCCCCAA CCGCTTCTAC 
TTCCTCGAGG CCTATAATGC AAAGAGCAAA AGCTTTGAAG ATCCTCCCAA CCATGCCCGT 
AGCCCTGGAA ACAAAGGGAA GGGCAAGGGA AAAGGGAAGG GCAAGCCCAA GTCCCAAGCC 
TGTGAGCCGA GCGAGCCAGA GATAGAGATC AAGCTGCCCA AGCTGCGGAC CCTGGATGTG 
TTTTCTGGCT GCGGGGGGTT GTCGGAGGGA TTCCACCAAG CAGGCATCTC TGACACGCTG 
TGGGCCATCG AGATGTGGGA CCCTGCGGCC CAGGCGTTCC GGCTGAACAA CCCCGGCTCC 
ACAGTGTTCA CAGAGGACTG CAACATCCTG CTGAAGCTGG TCATGGCTGG GGAGACCACC 
AACTCCCGCG GCCAGCGGCT GCCCCAGAAG GGAGACGTGG AGATGCTGTG CGGCGGGCCG 
CCCTGCCAGG GCTTCAGCGG CATGAACCGC TTCAATTCGC GCACCTACTC CAAGTTCAAA 
AACTCTCTGG TGGTTTCCTT CCTCAGCTAC TGCGACTACT ACCGGCCCCG GTTCTTCCTC 
CTGGAGAATG TCAGGAACTT TGTCTCCTTC AAGCGCTCCA TGGTCCTGAA GCTCACCCTC 
CGCTGCCTGG TCCGCATGGG CTATCAGTGC ACCTTCGGCG TGCTGCAGGC CGGTCAGTAC 
GGCGTGGCCC AGACTAGGAG GCGGGCCATC ATCCTGGCCG CGGCCCCTGG AGAGAAGCTC 
CCTCTGTTCC CGGAGCCACT GCACGTGTTT GCTCCCCGGG CCTGCCAGCT GAGCGTGGTG 
GTGGATGACA AGAAGTTTGT GAGCAACATA ACCAGGTTGA GCTCGGGTCC TTTCCGGACC 
ATCACGGTGC GAGACACGAT GTCCGACCTG CCGGAGGTGC GGAATGGAGC CTCGGCACTG 
GAGATCTCCT ACAACGGGGA GCCTCAGTCC TGGTTCCAGA GGCAGCTCCG GGGCGCACAG 
TACCAGCCCA TCCTCAGGGA CCACATCTGT AAGGACATGA GTGCATTGGT GGCTGCCCGC 
ATGCGGCACA TCCCCTTGGC CCCAGGGTCA GACTGGCGCG ATCTGCCCAA CATCGAGGTG 
CGGCTCTCAG ACGGCACCAT GGCCAGGAAG CTGCGGTATA CCCACCATGA CAGGAAGAAC 
GGCCGCAGCA GCTCTGGGGC CCTCCGTGGG GTCTGCTCCT GCGTGGAAGC CGGCAAAGCC 
TGCGACCCCG CAGCCAGGCA GTTCAACACC CTCATCCCCT GGTGCCTGCC CCACACCGGG 
AACCGGCACA ACCACTGGGC TGGCCTCTAT GGAAGGCTCG AGTGGGACGG CTTCTTCAGC 
ACAACCGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCAGAGCAG 
CACCGTGTGG TGAGCGTGCG GGAGTGTGCC CGCTCCCAGG GCTTCCCTGA CACCTACCGG 
CTCTTCGGCA ACATCCTGGA CAAGCACCGG CAGGTGGGCA ATGCCGTGCC ACCGCCCCTG 
GCCAAAGCCA TTGGCTTGGA GATCAAGCTT TGTATGTTGG CCAAAGCCCG AGAGAGTGCC 
TCAGCTAAAA TAAAGGAGGA GGAAGCTGCT AAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNMT1 ( NM_001130823.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124295.1 Homo sapiens DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1, mRNA.

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CloneID	OHu27021
Clone ID Related Accession (Same CDS sequence)	NM_001130823.2 , NM_001130823.3 , NM_001130823.1
Accession Version	NM_001130823.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	DNA (cytosine-5)-methyltransferase 1 isoform a
Comment

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCGTTCA AGAGACCCTC CTGCCTCAGC CTCCCAAGTA 
ACTGGGATTA GAGCTGAACC TTCACCTAGC CCCAGGATTA CAAGGAAAAG CACCAGGCAA 
ACCACCATCA CATCTCATTT TGCAAAGGGC CCTGCCAAAC GGAAACCTCA GGAAGAGTCT 
GAAAGAGCCA AATCGGATGA GTCCATCAAG GAAGAAGACA AAGACCAGGA TGAGAAGAGA 
CGTAGAGTTA CATCCAGAGA ACGAGTTGCT AGACCGCTTC CTGCAGAAGA ACCTGAAAGA 
GCAAAATCAG GAACGCGCAC TGAAAAGGAA GAAGAAAGAG ATGAAAAAGA AGAAAAGAGA 
CTCCGAAGTC AAACCAAAGA ACCAACACCC AAACAGAAAC TGAAGGAGGA GCCGGACAGA 
GAAGCCAGGG CAGGCGTGCA GGCTGACGAG GACGAAGATG GAGACGAGAA AGATGAGAAG 
AAGCACAGAA GTCAACCCAA AGATCTAGCT GCCAAACGGA GGCCCGAAGA AAAAGAACCT 
GAAAAAGTAA ATCCACAGAT TTCTGATGAA AAAGACGAGG ATGAAAAGGA GGAGAAGAGA 
CGCAAAACGA CCCCCAAAGA ACCAACGGAG AAAAAAATGG CTCGCGCCAA AACAGTCATG 
AACTCCAAGA CCCACCCTCC CAAGTGCATT CAGTGCGGGC AGTACCTGGA CGACCCTGAC 
CTCAAATATG GGCAGCACCC ACCAGACGCG GTGGATGAGC CACAGATGCT GACAAATGAG 
AAGCTGTCCA TCTTTGATGC CAACGAGTCT GGCTTTGAGA GTTATGAGGC GCTTCCCCAG 
CACAAACTGA CCTGCTTCAG TGTGTACTGT AAGCACGGTC ACCTGTGTCC CATCGACACC 
GGCCTCATCG AGAAGAATAT CGAACTCTTC TTTTCTGGTT CAGCAAAACC AATCTATGAT 
GATGACCCAT CTCTTGAAGG TGGTGTTAAT GGCAAAAATC TTGGCCCCAT AAATGAATGG 
TGGATCACTG GCTTTGATGG AGGTGAAAAG GCCCTCATCG GCTTCAGCAC CTCATTTGCC 
GAATACATTC TGATGGATCC CAGTCCCGAG TATGCGCCCA TATTTGGGCT GATGCAGGAG 
AAGATCTACA TCAGCAAGAT TGTGGTGGAG TTCCTGCAGA GCAATTCCGA CTCGACCTAT 
GAGGACCTGA TCAACAAGAT CGAGACCACG GTTCCTCCTT CTGGCCTCAA CTTGAACCGC 
TTCACAGAGG ACTCCCTCCT GCGACACGCG CAGTTTGTGG TGGAGCAGGT GGAGAGTTAT 
GACGAGGCCG GGGACAGTGA TGAGCAGCCC ATCTTCCTGA CACCCTGCAT GCGGGACCTG 
ATCAAGCTGG CTGGGGTCAC GCTGGGACAG AGGCGAGCCC AGGCGAGGCG GCAGACCATC 
AGGCATTCTA CCAGGGAGAA GGACAGGGGA CCCACGAAAG CCACCACCAC CAAGCTGGTC 
TACCAGATCT TCGATACTTT CTTCGCAGAG CAAATTGAAA AGGATGACAG AGAAGACAAG 
GAGAACGCCT TTAAGCGCCG GCGATGTGGC GTCTGTGAGG TGTGTCAGCA GCCTGAGTGT 
GGGAAATGTA AAGCCTGCAA GGACATGGTT AAATTTGGTG GCAGTGGACG GAGCAAGCAG 
GCTTGCCAAG AGCGGAGGTG TCCCAATATG GCCATGAAGG AGGCAGATGA CGATGAGGAA 
GTCGATGATA ACATCCCAGA GATGCCGTCA CCCAAAAAAA TGCACCAGGG GAAGAAGAAG 
AAACAGAACA AGAATCGCAT CTCTTGGGTC GGAGAAGCCG TCAAGACTGA TGGGAAGAAG 
AGTTACTATA AGAAGGTGTG CATTGATGCG GAAACCCTGG AAGTGGGGGA CTGTGTCTCT 
GTTATTCCAG ATGATTCCTC AAAACCGCTG TATCTAGCAA GGGTCACGGC GCTGTGGGAG 
GACAGCAGCA ACGGGCAGAT GTTTCACGCC CACTGGTTCT GCGCTGGGAC AGACACAGTC 
CTCGGGGCCA CGTCGGACCC TCTGGAGCTG TTCTTGGTGG ATGAATGTGA GGACATGCAG 
CTTTCATATA TCCACAGCAA AGTGAAAGTC ATCTACAAAG CCCCCTCCGA AAACTGGGCC 
ATGGAGGGAG GCATGGATCC CGAGTCCCTG CTGGAGGGGG ACGACGGGAA GACCTACTTC 
TACCAGCTGT GGTATGATCA AGACTACGCG AGATTCGAGT CCCCTCCAAA AACCCAGCCA 
ACAGAGGACA ACAAGTTCAA ATTCTGTGTG AGCTGTGCCC GTCTGGCTGA GATGAGGCAA 
AAAGAAATCC CCAGGGTCCT GGAGCAGCTC GAGGACCTGG ATAGCCGGGT CCTCTACTAC 
TCAGCCACCA AGAACGGCAT CCTGTACCGA GTTGGTGATG GTGTGTACCT GCCCCCTGAG 
GCCTTCACGT TCAACATCAA GCTGTCCAGT CCCGTGAAAC GCCCACGGAA GGAGCCCGTG 
GATGAGGACC TGTACCCAGA GCACTACCGG AAATACTCCG ACTACATCAA AGGCAGCAAC 
CTGGATGCCC CTGAGCCCTA CCGAATTGGC CGGATCAAAG AGATCTTCTG TCCCAAGAAG 
AGCAACGGCA GGCCCAATGA GACTGACATC AAAATCCGGG TCAACAAGTT CTACAGGCCT 
GAGAACACCC ACAAGTCCAC TCCAGCGAGC TACCACGCAG ACATCAACCT GCTCTACTGG 
AGCGACGAGG AGGCCGTGGT GGACTTCAAG GCTGTGCAGG GCCGCTGCAC CGTGGAGTAT 
GGGGAGGACC TGCCCGAGTG CGTCCAGGTG TACTCCATGG GCGGCCCCAA CCGCTTCTAC 
TTCCTCGAGG CCTATAATGC AAAGAGCAAA AGCTTTGAAG ATCCTCCCAA CCATGCCCGT 
AGCCCTGGAA ACAAAGGGAA GGGCAAGGGA AAAGGGAAGG GCAAGCCCAA GTCCCAAGCC 
TGTGAGCCGA GCGAGCCAGA GATAGAGATC AAGCTGCCCA AGCTGCGGAC CCTGGATGTG 
TTTTCTGGCT GCGGGGGGTT GTCGGAGGGA TTCCACCAAG CAGGCATCTC TGACACGCTG 
TGGGCCATCG AGATGTGGGA CCCTGCGGCC CAGGCGTTCC GGCTGAACAA CCCCGGCTCC 
ACAGTGTTCA CAGAGGACTG CAACATCCTG CTGAAGCTGG TCATGGCTGG GGAGACCACC 
AACTCCCGCG GCCAGCGGCT GCCCCAGAAG GGAGACGTGG AGATGCTGTG CGGCGGGCCG 
CCCTGCCAGG GCTTCAGCGG CATGAACCGC TTCAATTCGC GCACCTACTC CAAGTTCAAA 
AACTCTCTGG TGGTTTCCTT CCTCAGCTAC TGCGACTACT ACCGGCCCCG GTTCTTCCTC 
CTGGAGAATG TCAGGAACTT TGTCTCCTTC AAGCGCTCCA TGGTCCTGAA GCTCACCCTC 
CGCTGCCTGG TCCGCATGGG CTATCAGTGC ACCTTCGGCG TGCTGCAGGC CGGTCAGTAC 
GGCGTGGCCC AGACTAGGAG GCGGGCCATC ATCCTGGCCG CGGCCCCTGG AGAGAAGCTC 
CCTCTGTTCC CGGAGCCACT GCACGTGTTT GCTCCCCGGG CCTGCCAGCT GAGCGTGGTG 
GTGGATGACA AGAAGTTTGT GAGCAACATA ACCAGGTTGA GCTCGGGTCC TTTCCGGACC 
ATCACGGTGC GAGACACGAT GTCCGACCTG CCGGAGGTGC GGAATGGAGC CTCGGCACTG 
GAGATCTCCT ACAACGGGGA GCCTCAGTCC TGGTTCCAGA GGCAGCTCCG GGGCGCACAG 
TACCAGCCCA TCCTCAGGGA CCACATCTGT AAGGACATGA GTGCATTGGT GGCTGCCCGC 
ATGCGGCACA TCCCCTTGGC CCCAGGGTCA GACTGGCGCG ATCTGCCCAA CATCGAGGTG 
CGGCTCTCAG ACGGCACCAT GGCCAGGAAG CTGCGGTATA CCCACCATGA CAGGAAGAAC 
GGCCGCAGCA GCTCTGGGGC CCTCCGTGGG GTCTGCTCCT GCGTGGAAGC CGGCAAAGCC 
TGCGACCCCG CAGCCAGGCA GTTCAACACC CTCATCCCCT GGTGCCTGCC CCACACCGGG 
AACCGGCACA ACCACTGGGC TGGCCTCTAT GGAAGGCTCG AGTGGGACGG CTTCTTCAGC 
ACAACCGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCAGAGCAG 
CACCGTGTGG TGAGCGTGCG GGAGTGTGCC CGCTCCCAGG GCTTCCCTGA CACCTACCGG 
CTCTTCGGCA ACATCCTGGA CAAGCACCGG CAGGTGGGCA ATGCCGTGCC ACCGCCCCTG 
GCCAAAGCCA TTGGCTTGGA GATCAAGCTT TGTATGTTGG CCAAAGCCCG AGAGAGTGCC 
TCAGCTAAAA TAAAGGAGGA GGAAGCTGCT AAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_001124295.1

Translation

MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKRSRDPPASASQVTGIRAEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

Target ORF information:

RefSeq Version	NM_001130823.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130823.1

ORF Insert Sequence:

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCGTTCA AGAGACCCTC CTGCCTCAGC CTCCCAAGTA 
ACTGGGATTA GAGCTGAACC TTCACCTAGC CCCAGGATTA CAAGGAAAAG CACCAGGCAA 
ACCACCATCA CATCTCATTT TGCAAAGGGC CCTGCCAAAC GGAAACCTCA GGAAGAGTCT 
GAAAGAGCCA AATCGGATGA GTCCATCAAG GAAGAAGACA AAGACCAGGA TGAGAAGAGA 
CGTAGAGTTA CATCCAGAGA ACGAGTTGCT AGACCGCTTC CTGCAGAAGA ACCTGAAAGA 
GCAAAATCAG GAACGCGCAC TGAAAAGGAA GAAGAAAGAG ATGAAAAAGA AGAAAAGAGA 
CTCCGAAGTC AAACCAAAGA ACCAACACCC AAACAGAAAC TGAAGGAGGA GCCGGACAGA 
GAAGCCAGGG CAGGCGTGCA GGCTGACGAG GACGAAGATG GAGACGAGAA AGATGAGAAG 
AAGCACAGAA GTCAACCCAA AGATCTAGCT GCCAAACGGA GGCCCGAAGA AAAAGAACCT 
GAAAAAGTAA ATCCACAGAT TTCTGATGAA AAAGACGAGG ATGAAAAGGA GGAGAAGAGA 
CGCAAAACGA CCCCCAAAGA ACCAACGGAG AAAAAAATGG CTCGCGCCAA AACAGTCATG 
AACTCCAAGA CCCACCCTCC CAAGTGCATT CAGTGCGGGC AGTACCTGGA CGACCCTGAC 
CTCAAATATG GGCAGCACCC ACCAGACGCG GTGGATGAGC CACAGATGCT GACAAATGAG 
AAGCTGTCCA TCTTTGATGC CAACGAGTCT GGCTTTGAGA GTTATGAGGC GCTTCCCCAG 
CACAAACTGA CCTGCTTCAG TGTGTACTGT AAGCACGGTC ACCTGTGTCC CATCGACACC 
GGCCTCATCG AGAAGAATAT CGAACTCTTC TTTTCTGGTT CAGCAAAACC AATCTATGAT 
GATGACCCAT CTCTTGAAGG TGGTGTTAAT GGCAAAAATC TTGGCCCCAT AAATGAATGG 
TGGATCACTG GCTTTGATGG AGGTGAAAAG GCCCTCATCG GCTTCAGCAC CTCATTTGCC 
GAATACATTC TGATGGATCC CAGTCCCGAG TATGCGCCCA TATTTGGGCT GATGCAGGAG 
AAGATCTACA TCAGCAAGAT TGTGGTGGAG TTCCTGCAGA GCAATTCCGA CTCGACCTAT 
GAGGACCTGA TCAACAAGAT CGAGACCACG GTTCCTCCTT CTGGCCTCAA CTTGAACCGC 
TTCACAGAGG ACTCCCTCCT GCGACACGCG CAGTTTGTGG TGGAGCAGGT GGAGAGTTAT 
GACGAGGCCG GGGACAGTGA TGAGCAGCCC ATCTTCCTGA CACCCTGCAT GCGGGACCTG 
ATCAAGCTGG CTGGGGTCAC GCTGGGACAG AGGCGAGCCC AGGCGAGGCG GCAGACCATC 
AGGCATTCTA CCAGGGAGAA GGACAGGGGA CCCACGAAAG CCACCACCAC CAAGCTGGTC 
TACCAGATCT TCGATACTTT CTTCGCAGAG CAAATTGAAA AGGATGACAG AGAAGACAAG 
GAGAACGCCT TTAAGCGCCG GCGATGTGGC GTCTGTGAGG TGTGTCAGCA GCCTGAGTGT 
GGGAAATGTA AAGCCTGCAA GGACATGGTT AAATTTGGTG GCAGTGGACG GAGCAAGCAG 
GCTTGCCAAG AGCGGAGGTG TCCCAATATG GCCATGAAGG AGGCAGATGA CGATGAGGAA 
GTCGATGATA ACATCCCAGA GATGCCGTCA CCCAAAAAAA TGCACCAGGG GAAGAAGAAG 
AAACAGAACA AGAATCGCAT CTCTTGGGTC GGAGAAGCCG TCAAGACTGA TGGGAAGAAG 
AGTTACTATA AGAAGGTGTG CATTGATGCG GAAACCCTGG AAGTGGGGGA CTGTGTCTCT 
GTTATTCCAG ATGATTCCTC AAAACCGCTG TATCTAGCAA GGGTCACGGC GCTGTGGGAG 
GACAGCAGCA ACGGGCAGAT GTTTCACGCC CACTGGTTCT GCGCTGGGAC AGACACAGTC 
CTCGGGGCCA CGTCGGACCC TCTGGAGCTG TTCTTGGTGG ATGAATGTGA GGACATGCAG 
CTTTCATATA TCCACAGCAA AGTGAAAGTC ATCTACAAAG CCCCCTCCGA AAACTGGGCC 
ATGGAGGGAG GCATGGATCC CGAGTCCCTG CTGGAGGGGG ACGACGGGAA GACCTACTTC 
TACCAGCTGT GGTATGATCA AGACTACGCG AGATTCGAGT CCCCTCCAAA AACCCAGCCA 
ACAGAGGACA ACAAGTTCAA ATTCTGTGTG AGCTGTGCCC GTCTGGCTGA GATGAGGCAA 
AAAGAAATCC CCAGGGTCCT GGAGCAGCTC GAGGACCTGG ATAGCCGGGT CCTCTACTAC 
TCAGCCACCA AGAACGGCAT CCTGTACCGA GTTGGTGATG GTGTGTACCT GCCCCCTGAG 
GCCTTCACGT TCAACATCAA GCTGTCCAGT CCCGTGAAAC GCCCACGGAA GGAGCCCGTG 
GATGAGGACC TGTACCCAGA GCACTACCGG AAATACTCCG ACTACATCAA AGGCAGCAAC 
CTGGATGCCC CTGAGCCCTA CCGAATTGGC CGGATCAAAG AGATCTTCTG TCCCAAGAAG 
AGCAACGGCA GGCCCAATGA GACTGACATC AAAATCCGGG TCAACAAGTT CTACAGGCCT 
GAGAACACCC ACAAGTCCAC TCCAGCGAGC TACCACGCAG ACATCAACCT GCTCTACTGG 
AGCGACGAGG AGGCCGTGGT GGACTTCAAG GCTGTGCAGG GCCGCTGCAC CGTGGAGTAT 
GGGGAGGACC TGCCCGAGTG CGTCCAGGTG TACTCCATGG GCGGCCCCAA CCGCTTCTAC 
TTCCTCGAGG CCTATAATGC AAAGAGCAAA AGCTTTGAAG ATCCTCCCAA CCATGCCCGT 
AGCCCTGGAA ACAAAGGGAA GGGCAAGGGA AAAGGGAAGG GCAAGCCCAA GTCCCAAGCC 
TGTGAGCCGA GCGAGCCAGA GATAGAGATC AAGCTGCCCA AGCTGCGGAC CCTGGATGTG 
TTTTCTGGCT GCGGGGGGTT GTCGGAGGGA TTCCACCAAG CAGGCATCTC TGACACGCTG 
TGGGCCATCG AGATGTGGGA CCCTGCGGCC CAGGCGTTCC GGCTGAACAA CCCCGGCTCC 
ACAGTGTTCA CAGAGGACTG CAACATCCTG CTGAAGCTGG TCATGGCTGG GGAGACCACC 
AACTCCCGCG GCCAGCGGCT GCCCCAGAAG GGAGACGTGG AGATGCTGTG CGGCGGGCCG 
CCCTGCCAGG GCTTCAGCGG CATGAACCGC TTCAATTCGC GCACCTACTC CAAGTTCAAA 
AACTCTCTGG TGGTTTCCTT CCTCAGCTAC TGCGACTACT ACCGGCCCCG GTTCTTCCTC 
CTGGAGAATG TCAGGAACTT TGTCTCCTTC AAGCGCTCCA TGGTCCTGAA GCTCACCCTC 
CGCTGCCTGG TCCGCATGGG CTATCAGTGC ACCTTCGGCG TGCTGCAGGC CGGTCAGTAC 
GGCGTGGCCC AGACTAGGAG GCGGGCCATC ATCCTGGCCG CGGCCCCTGG AGAGAAGCTC 
CCTCTGTTCC CGGAGCCACT GCACGTGTTT GCTCCCCGGG CCTGCCAGCT GAGCGTGGTG 
GTGGATGACA AGAAGTTTGT GAGCAACATA ACCAGGTTGA GCTCGGGTCC TTTCCGGACC 
ATCACGGTGC GAGACACGAT GTCCGACCTG CCGGAGGTGC GGAATGGAGC CTCGGCACTG 
GAGATCTCCT ACAACGGGGA GCCTCAGTCC TGGTTCCAGA GGCAGCTCCG GGGCGCACAG 
TACCAGCCCA TCCTCAGGGA CCACATCTGT AAGGACATGA GTGCATTGGT GGCTGCCCGC 
ATGCGGCACA TCCCCTTGGC CCCAGGGTCA GACTGGCGCG ATCTGCCCAA CATCGAGGTG 
CGGCTCTCAG ACGGCACCAT GGCCAGGAAG CTGCGGTATA CCCACCATGA CAGGAAGAAC 
GGCCGCAGCA GCTCTGGGGC CCTCCGTGGG GTCTGCTCCT GCGTGGAAGC CGGCAAAGCC 
TGCGACCCCG CAGCCAGGCA GTTCAACACC CTCATCCCCT GGTGCCTGCC CCACACCGGG 
AACCGGCACA ACCACTGGGC TGGCCTCTAT GGAAGGCTCG AGTGGGACGG CTTCTTCAGC 
ACAACCGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCAGAGCAG 
CACCGTGTGG TGAGCGTGCG GGAGTGTGCC CGCTCCCAGG GCTTCCCTGA CACCTACCGG 
CTCTTCGGCA ACATCCTGGA CAAGCACCGG CAGGTGGGCA ATGCCGTGCC ACCGCCCCTG 
GCCAAAGCCA TTGGCTTGGA GATCAAGCTT TGTATGTTGG CCAAAGCCCG AGAGAGTGCC 
TCAGCTAAAA TAAAGGAGGA GGAAGCTGCT AAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNMT1 ( NM_001318731.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305660.1 Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 4, mRNA.

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CloneID	OHu102073
Clone ID Related Accession (Same CDS sequence)	NM_001318731.1 , NM_001318731.2
Accession Version	NM_001318731.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	DNA (cytosine-5)-methyltransferase 1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX379258.2, BC092517.1, X63692.1, DA653750.1 and HY245803.1. Transcript Variant: This variant (4) has a shorter 5' UTR, uses an alternate splice site in the 5' region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (d) has a shorter N-terminus than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC092517.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGATG CCAACAGCCC CCCCAAACCC CTTTCCAAAC CTCGCACGCC CAGGAGGAGC 
AAGTCCGATG GAGAGGCTAA GCGTTCAAGA GACCCTCCTG CCTCAGCCTC CCAAGTAACT 
GGGATTAGAG CTGAACCTTC ACCTAGCCCC AGGATTACAA GGAAAAGCAC CAGGCAAACC 
ACCATCACAT CTCATTTTGC AAAGGGCCCT GCCAAACGGA AACCTCAGGA AGAGTCTGAA 
AGAGCCAAAT CGGATGAGTC CATCAAGGAA GAAGACAAAG ACCAGGATGA GAAGAGACGT 
AGAGTTACAT CCAGAGAACG AGTTGCTAGA CCGCTTCCTG CAGAAGAACC TGAAAGAGCA 
AAATCAGGAA CGCGCACTGA AAAGGAAGAA GAAAGAGATG AAAAAGAAGA AAAGAGACTC 
CGAAGTCAAA CCAAAGAACC AACACCCAAA CAGAAACTGA AGGAGGAGCC GGACAGAGAA 
GCCAGGGCAG GCGTGCAGGC TGACGAGGAC GAAGATGGAG ACGAGAAAGA TGAGAAGAAG 
CACAGAAGTC AACCCAAAGA TCTAGCTGCC AAACGGAGGC CCGAAGAAAA AGAACCTGAA 
AAAGTAAATC CACAGATTTC TGATGAAAAA GACGAGGATG AAAAGGAGGA GAAGAGACGC 
AAAACGACCC CCAAAGAACC AACGGAGAAA AAAATGGCTC GCGCCAAAAC AGTCATGAAC 
TCCAAGACCC ACCCTCCCAA GTGCATTCAG TGCGGGCAGT ACCTGGACGA CCCTGACCTC 
AAATATGGGC AGCACCCACC AGACGCGGTG GATGAGCCAC AGATGCTGAC AAATGAGAAG 
CTGTCCATCT TTGATGCCAA CGAGTCTGGC TTTGAGAGTT ATGAGGCGCT TCCCCAGCAC 
AAACTGACCT GCTTCAGTGT GTACTGTAAG CACGGTCACC TGTGTCCCAT CGACACCGGC 
CTCATCGAGA AGAATATCGA ACTCTTCTTT TCTGGTTCAG CAAAACCAAT CTATGATGAT 
GACCCATCTC TTGAAGGTGG TGTTAATGGC AAAAATCTTG GCCCCATAAA TGAATGGTGG 
ATCACTGGCT TTGATGGAGG TGAAAAGGCC CTCATCGGCT TCAGCACCTC ATTTGCCGAA 
TACATTCTGA TGGATCCCAG TCCCGAGTAT GCGCCCATAT TTGGGCTGAT GCAGGAGAAG 
ATCTACATCA GCAAGATTGT GGTGGAGTTC CTGCAGAGCA ATTCCGACTC GACCTATGAG 
GACCTGATCA ACAAGATCGA GACCACGGTT CCTCCTTCTG GCCTCAACTT GAACCGCTTC 
ACAGAGGACT CCCTCCTGCG ACACGCGCAG TTTGTGGTGG AGCAGGTGGA GAGTTATGAC 
GAGGCCGGGG ACAGTGATGA GCAGCCCATC TTCCTGACAC CCTGCATGCG GGACCTGATC 
AAGCTGGCTG GGGTCACGCT GGGACAGAGG CGAGCCCAGG CGAGGCGGCA GACCATCAGG 
CATTCTACCA GGGAGAAGGA CAGGGGACCC ACGAAAGCCA CCACCACCAA GCTGGTCTAC 
CAGATCTTCG ATACTTTCTT CGCAGAGCAA ATTGAAAAGG ATGACAGAGA AGACAAGGAG 
AACGCCTTTA AGCGCCGGCG ATGTGGCGTC TGTGAGGTGT GTCAGCAGCC TGAGTGTGGG 
AAATGTAAAG CCTGCAAGGA CATGGTTAAA TTTGGTGGCA GTGGACGGAG CAAGCAGGCT 
TGCCAAGAGC GGAGGTGTCC CAATATGGCC ATGAAGGAGG CAGATGACGA TGAGGAAGTC 
GATGATAACA TCCCAGAGAT GCCGTCACCC AAAAAAATGC ACCAGGGGAA GAAGAAGAAA 
CAGAACAAGA ATCGCATCTC TTGGGTCGGA GAAGCCGTCA AGACTGATGG GAAGAAGAGT 
TACTATAAGA AGGTGTGCAT TGATGCGGAA ACCCTGGAAG TGGGGGACTG TGTCTCTGTT 
ATTCCAGATG ATTCCTCAAA ACCGCTGTAT CTAGCAAGGG TCACGGCGCT GTGGGAGGAC 
AGCAGCAACG GGCAGATGTT TCACGCCCAC TGGTTCTGCG CTGGGACAGA CACAGTCCTC 
GGGGCCACGT CGGACCCTCT GGAGCTGTTC TTGGTGGATG AATGTGAGGA CATGCAGCTT 
TCATATATCC ACAGCAAAGT GAAAGTCATC TACAAAGCCC CCTCCGAAAA CTGGGCCATG 
GAGGGAGGCA TGGATCCCGA GTCCCTGCTG GAGGGGGACG ACGGGAAGAC CTACTTCTAC 
CAGCTGTGGT ATGATCAAGA CTACGCGAGA TTCGAGTCCC CTCCAAAAAC CCAGCCAACA 
GAGGACAACA AGTTCAAATT CTGTGTGAGC TGTGCCCGTC TGGCTGAGAT GAGGCAAAAA 
GAAATCCCCA GGGTCCTGGA GCAGCTCGAG GACCTGGATA GCCGGGTCCT CTACTACTCA 
GCCACCAAGA ACGGCATCCT GTACCGAGTT GGTGATGGTG TGTACCTGCC CCCTGAGGCC 
TTCACGTTCA ACATCAAGCT GTCCAGTCCC GTGAAACGCC CACGGAAGGA GCCCGTGGAT 
GAGGACCTGT ACCCAGAGCA CTACCGGAAA TACTCCGACT ACATCAAAGG CAGCAACCTG 
GATGCCCCTG AGCCCTACCG AATTGGCCGG ATCAAAGAGA TCTTCTGTCC CAAGAAGAGC 
AACGGCAGGC CCAATGAGAC TGACATCAAA ATCCGGGTCA ACAAGTTCTA CAGGCCTGAG 
AACACCCACA AGTCCACTCC AGCGAGCTAC CACGCAGACA TCAACCTGCT CTACTGGAGC 
GACGAGGAGG CCGTGGTGGA CTTCAAGGCT GTGCAGGGCC GCTGCACCGT GGAGTATGGG 
GAGGACCTGC CCGAGTGCGT CCAGGTGTAC TCCATGGGCG GCCCCAACCG CTTCTACTTC 
CTCGAGGCCT ATAATGCAAA GAGCAAAAGC TTTGAAGATC CTCCCAACCA TGCCCGTAGC 
CCTGGAAACA AAGGGAAGGG CAAGGGAAAA GGGAAGGGCA AGCCCAAGTC CCAAGCCTGT 
GAGCCGAGCG AGCCAGAGAT AGAGATCAAG CTGCCCAAGC TGCGGACCCT GGATGTGTTT 
TCTGGCTGCG GGGGGTTGTC GGAGGGATTC CACCAAGCAG GCATCTCTGA CACGCTGTGG 
GCCATCGAGA TGTGGGACCC TGCGGCCCAG GCGTTCCGGC TGAACAACCC CGGCTCCACA 
GTGTTCACAG AGGACTGCAA CATCCTGCTG AAGCTGGTCA TGGCTGGGGA GACCACCAAC 
TCCCGCGGCC AGCGGCTGCC CCAGAAGGGA GACGTGGAGA TGCTGTGCGG CGGGCCGCCC 
TGCCAGGGCT TCAGCGGCAT GAACCGCTTC AATTCGCGCA CCTACTCCAA GTTCAAAAAC 
TCTCTGGTGG TTTCCTTCCT CAGCTACTGC GACTACTACC GGCCCCGGTT CTTCCTCCTG 
GAGAATGTCA GGAACTTTGT CTCCTTCAAG CGCTCCATGG TCCTGAAGCT CACCCTCCGC 
TGCCTGGTCC GCATGGGCTA TCAGTGCACC TTCGGCGTGC TGCAGGCCGG TCAGTACGGC 
GTGGCCCAGA CTAGGAGGCG GGCCATCATC CTGGCCGCGG CCCCTGGAGA GAAGCTCCCT 
CTGTTCCCGG AGCCACTGCA CGTGTTTGCT CCCCGGGCCT GCCAGCTGAG CGTGGTGGTG 
GATGACAAGA AGTTTGTGAG CAACATAACC AGGTTGAGCT CGGGTCCTTT CCGGACCATC 
ACGGTGCGAG ACACGATGTC CGACCTGCCG GAGGTGCGGA ATGGAGCCTC GGCACTGGAG 
ATCTCCTACA ACGGGGAGCC TCAGTCCTGG TTCCAGAGGC AGCTCCGGGG CGCACAGTAC 
CAGCCCATCC TCAGGGACCA CATCTGTAAG GACATGAGTG CATTGGTGGC TGCCCGCATG 
CGGCACATCC CCTTGGCCCC AGGGTCAGAC TGGCGCGATC TGCCCAACAT CGAGGTGCGG 
CTCTCAGACG GCACCATGGC CAGGAAGCTG CGGTATACCC ACCATGACAG GAAGAACGGC 
CGCAGCAGCT CTGGGGCCCT CCGTGGGGTC TGCTCCTGCG TGGAAGCCGG CAAAGCCTGC 
GACCCCGCAG CCAGGCAGTT CAACACCCTC ATCCCCTGGT GCCTGCCCCA CACCGGGAAC 
CGGCACAACC ACTGGGCTGG CCTCTATGGA AGGCTCGAGT GGGACGGCTT CTTCAGCACA 
ACCGTCACCA ACCCCGAGCC CATGGGCAAG CAGGGCCGCG TGCTCCACCC AGAGCAGCAC 
CGTGTGGTGA GCGTGCGGGA GTGTGCCCGC TCCCAGGGCT TCCCTGACAC CTACCGGCTC 
TTCGGCAACA TCCTGGACAA GCACCGGCAG GTGGGCAATG CCGTGCCACC GCCCCTGGCC 
AAAGCCATTG GCTTGGAGAT CAAGCTTTGT ATGTTGGCCA AAGCCCGAGA GAGTGCCTCA 
GCTAAAATAA AGGAGGAGGA AGCTGCTAAG GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305660.1
CDS	479..5014
Translation	MADANSPPKPLSKPRTPRRSKSDGEAKRSRDPPASASQVTGIRAEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

Target ORF information:

RefSeq Version	NM_001318731.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318731.1

ORF Insert Sequence:

ATGGCAGATG CCAACAGCCC CCCCAAACCC CTTTCCAAAC CTCGCACGCC CAGGAGGAGC 
AAGTCCGATG GAGAGGCTAA GCGTTCAAGA GACCCTCCTG CCTCAGCCTC CCAAGTAACT 
GGGATTAGAG CTGAACCTTC ACCTAGCCCC AGGATTACAA GGAAAAGCAC CAGGCAAACC 
ACCATCACAT CTCATTTTGC AAAGGGCCCT GCCAAACGGA AACCTCAGGA AGAGTCTGAA 
AGAGCCAAAT CGGATGAGTC CATCAAGGAA GAAGACAAAG ACCAGGATGA GAAGAGACGT 
AGAGTTACAT CCAGAGAACG AGTTGCTAGA CCGCTTCCTG CAGAAGAACC TGAAAGAGCA 
AAATCAGGAA CGCGCACTGA AAAGGAAGAA GAAAGAGATG AAAAAGAAGA AAAGAGACTC 
CGAAGTCAAA CCAAAGAACC AACACCCAAA CAGAAACTGA AGGAGGAGCC GGACAGAGAA 
GCCAGGGCAG GCGTGCAGGC TGACGAGGAC GAAGATGGAG ACGAGAAAGA TGAGAAGAAG 
CACAGAAGTC AACCCAAAGA TCTAGCTGCC AAACGGAGGC CCGAAGAAAA AGAACCTGAA 
AAAGTAAATC CACAGATTTC TGATGAAAAA GACGAGGATG AAAAGGAGGA GAAGAGACGC 
AAAACGACCC CCAAAGAACC AACGGAGAAA AAAATGGCTC GCGCCAAAAC AGTCATGAAC 
TCCAAGACCC ACCCTCCCAA GTGCATTCAG TGCGGGCAGT ACCTGGACGA CCCTGACCTC 
AAATATGGGC AGCACCCACC AGACGCGGTG GATGAGCCAC AGATGCTGAC AAATGAGAAG 
CTGTCCATCT TTGATGCCAA CGAGTCTGGC TTTGAGAGTT ATGAGGCGCT TCCCCAGCAC 
AAACTGACCT GCTTCAGTGT GTACTGTAAG CACGGTCACC TGTGTCCCAT CGACACCGGC 
CTCATCGAGA AGAATATCGA ACTCTTCTTT TCTGGTTCAG CAAAACCAAT CTATGATGAT 
GACCCATCTC TTGAAGGTGG TGTTAATGGC AAAAATCTTG GCCCCATAAA TGAATGGTGG 
ATCACTGGCT TTGATGGAGG TGAAAAGGCC CTCATCGGCT TCAGCACCTC ATTTGCCGAA 
TACATTCTGA TGGATCCCAG TCCCGAGTAT GCGCCCATAT TTGGGCTGAT GCAGGAGAAG 
ATCTACATCA GCAAGATTGT GGTGGAGTTC CTGCAGAGCA ATTCCGACTC GACCTATGAG 
GACCTGATCA ACAAGATCGA GACCACGGTT CCTCCTTCTG GCCTCAACTT GAACCGCTTC 
ACAGAGGACT CCCTCCTGCG ACACGCGCAG TTTGTGGTGG AGCAGGTGGA GAGTTATGAC 
GAGGCCGGGG ACAGTGATGA GCAGCCCATC TTCCTGACAC CCTGCATGCG GGACCTGATC 
AAGCTGGCTG GGGTCACGCT GGGACAGAGG CGAGCCCAGG CGAGGCGGCA GACCATCAGG 
CATTCTACCA GGGAGAAGGA CAGGGGACCC ACGAAAGCCA CCACCACCAA GCTGGTCTAC 
CAGATCTTCG ATACTTTCTT CGCAGAGCAA ATTGAAAAGG ATGACAGAGA AGACAAGGAG 
AACGCCTTTA AGCGCCGGCG ATGTGGCGTC TGTGAGGTGT GTCAGCAGCC TGAGTGTGGG 
AAATGTAAAG CCTGCAAGGA CATGGTTAAA TTTGGTGGCA GTGGACGGAG CAAGCAGGCT 
TGCCAAGAGC GGAGGTGTCC CAATATGGCC ATGAAGGAGG CAGATGACGA TGAGGAAGTC 
GATGATAACA TCCCAGAGAT GCCGTCACCC AAAAAAATGC ACCAGGGGAA GAAGAAGAAA 
CAGAACAAGA ATCGCATCTC TTGGGTCGGA GAAGCCGTCA AGACTGATGG GAAGAAGAGT 
TACTATAAGA AGGTGTGCAT TGATGCGGAA ACCCTGGAAG TGGGGGACTG TGTCTCTGTT 
ATTCCAGATG ATTCCTCAAA ACCGCTGTAT CTAGCAAGGG TCACGGCGCT GTGGGAGGAC 
AGCAGCAACG GGCAGATGTT TCACGCCCAC TGGTTCTGCG CTGGGACAGA CACAGTCCTC 
GGGGCCACGT CGGACCCTCT GGAGCTGTTC TTGGTGGATG AATGTGAGGA CATGCAGCTT 
TCATATATCC ACAGCAAAGT GAAAGTCATC TACAAAGCCC CCTCCGAAAA CTGGGCCATG 
GAGGGAGGCA TGGATCCCGA GTCCCTGCTG GAGGGGGACG ACGGGAAGAC CTACTTCTAC 
CAGCTGTGGT ATGATCAAGA CTACGCGAGA TTCGAGTCCC CTCCAAAAAC CCAGCCAACA 
GAGGACAACA AGTTCAAATT CTGTGTGAGC TGTGCCCGTC TGGCTGAGAT GAGGCAAAAA 
GAAATCCCCA GGGTCCTGGA GCAGCTCGAG GACCTGGATA GCCGGGTCCT CTACTACTCA 
GCCACCAAGA ACGGCATCCT GTACCGAGTT GGTGATGGTG TGTACCTGCC CCCTGAGGCC 
TTCACGTTCA ACATCAAGCT GTCCAGTCCC GTGAAACGCC CACGGAAGGA GCCCGTGGAT 
GAGGACCTGT ACCCAGAGCA CTACCGGAAA TACTCCGACT ACATCAAAGG CAGCAACCTG 
GATGCCCCTG AGCCCTACCG AATTGGCCGG ATCAAAGAGA TCTTCTGTCC CAAGAAGAGC 
AACGGCAGGC CCAATGAGAC TGACATCAAA ATCCGGGTCA ACAAGTTCTA CAGGCCTGAG 
AACACCCACA AGTCCACTCC AGCGAGCTAC CACGCAGACA TCAACCTGCT CTACTGGAGC 
GACGAGGAGG CCGTGGTGGA CTTCAAGGCT GTGCAGGGCC GCTGCACCGT GGAGTATGGG 
GAGGACCTGC CCGAGTGCGT CCAGGTGTAC TCCATGGGCG GCCCCAACCG CTTCTACTTC 
CTCGAGGCCT ATAATGCAAA GAGCAAAAGC TTTGAAGATC CTCCCAACCA TGCCCGTAGC 
CCTGGAAACA AAGGGAAGGG CAAGGGAAAA GGGAAGGGCA AGCCCAAGTC CCAAGCCTGT 
GAGCCGAGCG AGCCAGAGAT AGAGATCAAG CTGCCCAAGC TGCGGACCCT GGATGTGTTT 
TCTGGCTGCG GGGGGTTGTC GGAGGGATTC CACCAAGCAG GCATCTCTGA CACGCTGTGG 
GCCATCGAGA TGTGGGACCC TGCGGCCCAG GCGTTCCGGC TGAACAACCC CGGCTCCACA 
GTGTTCACAG AGGACTGCAA CATCCTGCTG AAGCTGGTCA TGGCTGGGGA GACCACCAAC 
TCCCGCGGCC AGCGGCTGCC CCAGAAGGGA GACGTGGAGA TGCTGTGCGG CGGGCCGCCC 
TGCCAGGGCT TCAGCGGCAT GAACCGCTTC AATTCGCGCA CCTACTCCAA GTTCAAAAAC 
TCTCTGGTGG TTTCCTTCCT CAGCTACTGC GACTACTACC GGCCCCGGTT CTTCCTCCTG 
GAGAATGTCA GGAACTTTGT CTCCTTCAAG CGCTCCATGG TCCTGAAGCT CACCCTCCGC 
TGCCTGGTCC GCATGGGCTA TCAGTGCACC TTCGGCGTGC TGCAGGCCGG TCAGTACGGC 
GTGGCCCAGA CTAGGAGGCG GGCCATCATC CTGGCCGCGG CCCCTGGAGA GAAGCTCCCT 
CTGTTCCCGG AGCCACTGCA CGTGTTTGCT CCCCGGGCCT GCCAGCTGAG CGTGGTGGTG 
GATGACAAGA AGTTTGTGAG CAACATAACC AGGTTGAGCT CGGGTCCTTT CCGGACCATC 
ACGGTGCGAG ACACGATGTC CGACCTGCCG GAGGTGCGGA ATGGAGCCTC GGCACTGGAG 
ATCTCCTACA ACGGGGAGCC TCAGTCCTGG TTCCAGAGGC AGCTCCGGGG CGCACAGTAC 
CAGCCCATCC TCAGGGACCA CATCTGTAAG GACATGAGTG CATTGGTGGC TGCCCGCATG 
CGGCACATCC CCTTGGCCCC AGGGTCAGAC TGGCGCGATC TGCCCAACAT CGAGGTGCGG 
CTCTCAGACG GCACCATGGC CAGGAAGCTG CGGTATACCC ACCATGACAG GAAGAACGGC 
CGCAGCAGCT CTGGGGCCCT CCGTGGGGTC TGCTCCTGCG TGGAAGCCGG CAAAGCCTGC 
GACCCCGCAG CCAGGCAGTT CAACACCCTC ATCCCCTGGT GCCTGCCCCA CACCGGGAAC 
CGGCACAACC ACTGGGCTGG CCTCTATGGA AGGCTCGAGT GGGACGGCTT CTTCAGCACA 
ACCGTCACCA ACCCCGAGCC CATGGGCAAG CAGGGCCGCG TGCTCCACCC AGAGCAGCAC 
CGTGTGGTGA GCGTGCGGGA GTGTGCCCGC TCCCAGGGCT TCCCTGACAC CTACCGGCTC 
TTCGGCAACA TCCTGGACAA GCACCGGCAG GTGGGCAATG CCGTGCCACC GCCCCTGGCC 
AAAGCCATTG GCTTGGAGAT CAAGCTTTGT ATGTTGGCCA AAGCCCGAGA GAGTGCCTCA 
GCTAAAATAA AGGAGGAGGA AGCTGCTAAG GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNMT1 ( NM_001318731.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305660.1 Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 4, mRNA.

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CloneID	OHu102073
Clone ID Related Accession (Same CDS sequence)	NM_001318731.1 , NM_001318731.2
Accession Version	NM_001318731.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	DNA (cytosine-5)-methyltransferase 1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX379258.2, BC092517.1, X63692.1 and DA653750.1. On May 31, 2019 this sequence version replaced NM_001318731.1. Transcript Variant: This variant (4) has a shorter 5' UTR, uses an alternate splice site in the 5' region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (d) has a shorter N-terminus than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC092517.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGATG CCAACAGCCC CCCCAAACCC CTTTCCAAAC CTCGCACGCC CAGGAGGAGC 
AAGTCCGATG GAGAGGCTAA GCGTTCAAGA GACCCTCCTG CCTCAGCCTC CCAAGTAACT 
GGGATTAGAG CTGAACCTTC ACCTAGCCCC AGGATTACAA GGAAAAGCAC CAGGCAAACC 
ACCATCACAT CTCATTTTGC AAAGGGCCCT GCCAAACGGA AACCTCAGGA AGAGTCTGAA 
AGAGCCAAAT CGGATGAGTC CATCAAGGAA GAAGACAAAG ACCAGGATGA GAAGAGACGT 
AGAGTTACAT CCAGAGAACG AGTTGCTAGA CCGCTTCCTG CAGAAGAACC TGAAAGAGCA 
AAATCAGGAA CGCGCACTGA AAAGGAAGAA GAAAGAGATG AAAAAGAAGA AAAGAGACTC 
CGAAGTCAAA CCAAAGAACC AACACCCAAA CAGAAACTGA AGGAGGAGCC GGACAGAGAA 
GCCAGGGCAG GCGTGCAGGC TGACGAGGAC GAAGATGGAG ACGAGAAAGA TGAGAAGAAG 
CACAGAAGTC AACCCAAAGA TCTAGCTGCC AAACGGAGGC CCGAAGAAAA AGAACCTGAA 
AAAGTAAATC CACAGATTTC TGATGAAAAA GACGAGGATG AAAAGGAGGA GAAGAGACGC 
AAAACGACCC CCAAAGAACC AACGGAGAAA AAAATGGCTC GCGCCAAAAC AGTCATGAAC 
TCCAAGACCC ACCCTCCCAA GTGCATTCAG TGCGGGCAGT ACCTGGACGA CCCTGACCTC 
AAATATGGGC AGCACCCACC AGACGCGGTG GATGAGCCAC AGATGCTGAC AAATGAGAAG 
CTGTCCATCT TTGATGCCAA CGAGTCTGGC TTTGAGAGTT ATGAGGCGCT TCCCCAGCAC 
AAACTGACCT GCTTCAGTGT GTACTGTAAG CACGGTCACC TGTGTCCCAT CGACACCGGC 
CTCATCGAGA AGAATATCGA ACTCTTCTTT TCTGGTTCAG CAAAACCAAT CTATGATGAT 
GACCCATCTC TTGAAGGTGG TGTTAATGGC AAAAATCTTG GCCCCATAAA TGAATGGTGG 
ATCACTGGCT TTGATGGAGG TGAAAAGGCC CTCATCGGCT TCAGCACCTC ATTTGCCGAA 
TACATTCTGA TGGATCCCAG TCCCGAGTAT GCGCCCATAT TTGGGCTGAT GCAGGAGAAG 
ATCTACATCA GCAAGATTGT GGTGGAGTTC CTGCAGAGCA ATTCCGACTC GACCTATGAG 
GACCTGATCA ACAAGATCGA GACCACGGTT CCTCCTTCTG GCCTCAACTT GAACCGCTTC 
ACAGAGGACT CCCTCCTGCG ACACGCGCAG TTTGTGGTGG AGCAGGTGGA GAGTTATGAC 
GAGGCCGGGG ACAGTGATGA GCAGCCCATC TTCCTGACAC CCTGCATGCG GGACCTGATC 
AAGCTGGCTG GGGTCACGCT GGGACAGAGG CGAGCCCAGG CGAGGCGGCA GACCATCAGG 
CATTCTACCA GGGAGAAGGA CAGGGGACCC ACGAAAGCCA CCACCACCAA GCTGGTCTAC 
CAGATCTTCG ATACTTTCTT CGCAGAGCAA ATTGAAAAGG ATGACAGAGA AGACAAGGAG 
AACGCCTTTA AGCGCCGGCG ATGTGGCGTC TGTGAGGTGT GTCAGCAGCC TGAGTGTGGG 
AAATGTAAAG CCTGCAAGGA CATGGTTAAA TTTGGTGGCA GTGGACGGAG CAAGCAGGCT 
TGCCAAGAGC GGAGGTGTCC CAATATGGCC ATGAAGGAGG CAGATGACGA TGAGGAAGTC 
GATGATAACA TCCCAGAGAT GCCGTCACCC AAAAAAATGC ACCAGGGGAA GAAGAAGAAA 
CAGAACAAGA ATCGCATCTC TTGGGTCGGA GAAGCCGTCA AGACTGATGG GAAGAAGAGT 
TACTATAAGA AGGTGTGCAT TGATGCGGAA ACCCTGGAAG TGGGGGACTG TGTCTCTGTT 
ATTCCAGATG ATTCCTCAAA ACCGCTGTAT CTAGCAAGGG TCACGGCGCT GTGGGAGGAC 
AGCAGCAACG GGCAGATGTT TCACGCCCAC TGGTTCTGCG CTGGGACAGA CACAGTCCTC 
GGGGCCACGT CGGACCCTCT GGAGCTGTTC TTGGTGGATG AATGTGAGGA CATGCAGCTT 
TCATATATCC ACAGCAAAGT GAAAGTCATC TACAAAGCCC CCTCCGAAAA CTGGGCCATG 
GAGGGAGGCA TGGATCCCGA GTCCCTGCTG GAGGGGGACG ACGGGAAGAC CTACTTCTAC 
CAGCTGTGGT ATGATCAAGA CTACGCGAGA TTCGAGTCCC CTCCAAAAAC CCAGCCAACA 
GAGGACAACA AGTTCAAATT CTGTGTGAGC TGTGCCCGTC TGGCTGAGAT GAGGCAAAAA 
GAAATCCCCA GGGTCCTGGA GCAGCTCGAG GACCTGGATA GCCGGGTCCT CTACTACTCA 
GCCACCAAGA ACGGCATCCT GTACCGAGTT GGTGATGGTG TGTACCTGCC CCCTGAGGCC 
TTCACGTTCA ACATCAAGCT GTCCAGTCCC GTGAAACGCC CACGGAAGGA GCCCGTGGAT 
GAGGACCTGT ACCCAGAGCA CTACCGGAAA TACTCCGACT ACATCAAAGG CAGCAACCTG 
GATGCCCCTG AGCCCTACCG AATTGGCCGG ATCAAAGAGA TCTTCTGTCC CAAGAAGAGC 
AACGGCAGGC CCAATGAGAC TGACATCAAA ATCCGGGTCA ACAAGTTCTA CAGGCCTGAG 
AACACCCACA AGTCCACTCC AGCGAGCTAC CACGCAGACA TCAACCTGCT CTACTGGAGC 
GACGAGGAGG CCGTGGTGGA CTTCAAGGCT GTGCAGGGCC GCTGCACCGT GGAGTATGGG 
GAGGACCTGC CCGAGTGCGT CCAGGTGTAC TCCATGGGCG GCCCCAACCG CTTCTACTTC 
CTCGAGGCCT ATAATGCAAA GAGCAAAAGC TTTGAAGATC CTCCCAACCA TGCCCGTAGC 
CCTGGAAACA AAGGGAAGGG CAAGGGAAAA GGGAAGGGCA AGCCCAAGTC CCAAGCCTGT 
GAGCCGAGCG AGCCAGAGAT AGAGATCAAG CTGCCCAAGC TGCGGACCCT GGATGTGTTT 
TCTGGCTGCG GGGGGTTGTC GGAGGGATTC CACCAAGCAG GCATCTCTGA CACGCTGTGG 
GCCATCGAGA TGTGGGACCC TGCGGCCCAG GCGTTCCGGC TGAACAACCC CGGCTCCACA 
GTGTTCACAG AGGACTGCAA CATCCTGCTG AAGCTGGTCA TGGCTGGGGA GACCACCAAC 
TCCCGCGGCC AGCGGCTGCC CCAGAAGGGA GACGTGGAGA TGCTGTGCGG CGGGCCGCCC 
TGCCAGGGCT TCAGCGGCAT GAACCGCTTC AATTCGCGCA CCTACTCCAA GTTCAAAAAC 
TCTCTGGTGG TTTCCTTCCT CAGCTACTGC GACTACTACC GGCCCCGGTT CTTCCTCCTG 
GAGAATGTCA GGAACTTTGT CTCCTTCAAG CGCTCCATGG TCCTGAAGCT CACCCTCCGC 
TGCCTGGTCC GCATGGGCTA TCAGTGCACC TTCGGCGTGC TGCAGGCCGG TCAGTACGGC 
GTGGCCCAGA CTAGGAGGCG GGCCATCATC CTGGCCGCGG CCCCTGGAGA GAAGCTCCCT 
CTGTTCCCGG AGCCACTGCA CGTGTTTGCT CCCCGGGCCT GCCAGCTGAG CGTGGTGGTG 
GATGACAAGA AGTTTGTGAG CAACATAACC AGGTTGAGCT CGGGTCCTTT CCGGACCATC 
ACGGTGCGAG ACACGATGTC CGACCTGCCG GAGGTGCGGA ATGGAGCCTC GGCACTGGAG 
ATCTCCTACA ACGGGGAGCC TCAGTCCTGG TTCCAGAGGC AGCTCCGGGG CGCACAGTAC 
CAGCCCATCC TCAGGGACCA CATCTGTAAG GACATGAGTG CATTGGTGGC TGCCCGCATG 
CGGCACATCC CCTTGGCCCC AGGGTCAGAC TGGCGCGATC TGCCCAACAT CGAGGTGCGG 
CTCTCAGACG GCACCATGGC CAGGAAGCTG CGGTATACCC ACCATGACAG GAAGAACGGC 
CGCAGCAGCT CTGGGGCCCT CCGTGGGGTC TGCTCCTGCG TGGAAGCCGG CAAAGCCTGC 
GACCCCGCAG CCAGGCAGTT CAACACCCTC ATCCCCTGGT GCCTGCCCCA CACCGGGAAC 
CGGCACAACC ACTGGGCTGG CCTCTATGGA AGGCTCGAGT GGGACGGCTT CTTCAGCACA 
ACCGTCACCA ACCCCGAGCC CATGGGCAAG CAGGGCCGCG TGCTCCACCC AGAGCAGCAC 
CGTGTGGTGA GCGTGCGGGA GTGTGCCCGC TCCCAGGGCT TCCCTGACAC CTACCGGCTC 
TTCGGCAACA TCCTGGACAA GCACCGGCAG GTGGGCAATG CCGTGCCACC GCCCCTGGCC 
AAAGCCATTG GCTTGGAGAT CAAGCTTTGT ATGTTGGCCA AAGCCCGAGA GAGTGCCTCA 
GCTAAAATAA AGGAGGAGGA AGCTGCTAAG GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305660.1
CDS	378..4913
Translation	MADANSPPKPLSKPRTPRRSKSDGEAKRSRDPPASASQVTGIRAEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

Target ORF information:

RefSeq Version	NM_001318731.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318731.2

ORF Insert Sequence:

ATGGCAGATG CCAACAGCCC CCCCAAACCC CTTTCCAAAC CTCGCACGCC CAGGAGGAGC 
AAGTCCGATG GAGAGGCTAA GCGTTCAAGA GACCCTCCTG CCTCAGCCTC CCAAGTAACT 
GGGATTAGAG CTGAACCTTC ACCTAGCCCC AGGATTACAA GGAAAAGCAC CAGGCAAACC 
ACCATCACAT CTCATTTTGC AAAGGGCCCT GCCAAACGGA AACCTCAGGA AGAGTCTGAA 
AGAGCCAAAT CGGATGAGTC CATCAAGGAA GAAGACAAAG ACCAGGATGA GAAGAGACGT 
AGAGTTACAT CCAGAGAACG AGTTGCTAGA CCGCTTCCTG CAGAAGAACC TGAAAGAGCA 
AAATCAGGAA CGCGCACTGA AAAGGAAGAA GAAAGAGATG AAAAAGAAGA AAAGAGACTC 
CGAAGTCAAA CCAAAGAACC AACACCCAAA CAGAAACTGA AGGAGGAGCC GGACAGAGAA 
GCCAGGGCAG GCGTGCAGGC TGACGAGGAC GAAGATGGAG ACGAGAAAGA TGAGAAGAAG 
CACAGAAGTC AACCCAAAGA TCTAGCTGCC AAACGGAGGC CCGAAGAAAA AGAACCTGAA 
AAAGTAAATC CACAGATTTC TGATGAAAAA GACGAGGATG AAAAGGAGGA GAAGAGACGC 
AAAACGACCC CCAAAGAACC AACGGAGAAA AAAATGGCTC GCGCCAAAAC AGTCATGAAC 
TCCAAGACCC ACCCTCCCAA GTGCATTCAG TGCGGGCAGT ACCTGGACGA CCCTGACCTC 
AAATATGGGC AGCACCCACC AGACGCGGTG GATGAGCCAC AGATGCTGAC AAATGAGAAG 
CTGTCCATCT TTGATGCCAA CGAGTCTGGC TTTGAGAGTT ATGAGGCGCT TCCCCAGCAC 
AAACTGACCT GCTTCAGTGT GTACTGTAAG CACGGTCACC TGTGTCCCAT CGACACCGGC 
CTCATCGAGA AGAATATCGA ACTCTTCTTT TCTGGTTCAG CAAAACCAAT CTATGATGAT 
GACCCATCTC TTGAAGGTGG TGTTAATGGC AAAAATCTTG GCCCCATAAA TGAATGGTGG 
ATCACTGGCT TTGATGGAGG TGAAAAGGCC CTCATCGGCT TCAGCACCTC ATTTGCCGAA 
TACATTCTGA TGGATCCCAG TCCCGAGTAT GCGCCCATAT TTGGGCTGAT GCAGGAGAAG 
ATCTACATCA GCAAGATTGT GGTGGAGTTC CTGCAGAGCA ATTCCGACTC GACCTATGAG 
GACCTGATCA ACAAGATCGA GACCACGGTT CCTCCTTCTG GCCTCAACTT GAACCGCTTC 
ACAGAGGACT CCCTCCTGCG ACACGCGCAG TTTGTGGTGG AGCAGGTGGA GAGTTATGAC 
GAGGCCGGGG ACAGTGATGA GCAGCCCATC TTCCTGACAC CCTGCATGCG GGACCTGATC 
AAGCTGGCTG GGGTCACGCT GGGACAGAGG CGAGCCCAGG CGAGGCGGCA GACCATCAGG 
CATTCTACCA GGGAGAAGGA CAGGGGACCC ACGAAAGCCA CCACCACCAA GCTGGTCTAC 
CAGATCTTCG ATACTTTCTT CGCAGAGCAA ATTGAAAAGG ATGACAGAGA AGACAAGGAG 
AACGCCTTTA AGCGCCGGCG ATGTGGCGTC TGTGAGGTGT GTCAGCAGCC TGAGTGTGGG 
AAATGTAAAG CCTGCAAGGA CATGGTTAAA TTTGGTGGCA GTGGACGGAG CAAGCAGGCT 
TGCCAAGAGC GGAGGTGTCC CAATATGGCC ATGAAGGAGG CAGATGACGA TGAGGAAGTC 
GATGATAACA TCCCAGAGAT GCCGTCACCC AAAAAAATGC ACCAGGGGAA GAAGAAGAAA 
CAGAACAAGA ATCGCATCTC TTGGGTCGGA GAAGCCGTCA AGACTGATGG GAAGAAGAGT 
TACTATAAGA AGGTGTGCAT TGATGCGGAA ACCCTGGAAG TGGGGGACTG TGTCTCTGTT 
ATTCCAGATG ATTCCTCAAA ACCGCTGTAT CTAGCAAGGG TCACGGCGCT GTGGGAGGAC 
AGCAGCAACG GGCAGATGTT TCACGCCCAC TGGTTCTGCG CTGGGACAGA CACAGTCCTC 
GGGGCCACGT CGGACCCTCT GGAGCTGTTC TTGGTGGATG AATGTGAGGA CATGCAGCTT 
TCATATATCC ACAGCAAAGT GAAAGTCATC TACAAAGCCC CCTCCGAAAA CTGGGCCATG 
GAGGGAGGCA TGGATCCCGA GTCCCTGCTG GAGGGGGACG ACGGGAAGAC CTACTTCTAC 
CAGCTGTGGT ATGATCAAGA CTACGCGAGA TTCGAGTCCC CTCCAAAAAC CCAGCCAACA 
GAGGACAACA AGTTCAAATT CTGTGTGAGC TGTGCCCGTC TGGCTGAGAT GAGGCAAAAA 
GAAATCCCCA GGGTCCTGGA GCAGCTCGAG GACCTGGATA GCCGGGTCCT CTACTACTCA 
GCCACCAAGA ACGGCATCCT GTACCGAGTT GGTGATGGTG TGTACCTGCC CCCTGAGGCC 
TTCACGTTCA ACATCAAGCT GTCCAGTCCC GTGAAACGCC CACGGAAGGA GCCCGTGGAT 
GAGGACCTGT ACCCAGAGCA CTACCGGAAA TACTCCGACT ACATCAAAGG CAGCAACCTG 
GATGCCCCTG AGCCCTACCG AATTGGCCGG ATCAAAGAGA TCTTCTGTCC CAAGAAGAGC 
AACGGCAGGC CCAATGAGAC TGACATCAAA ATCCGGGTCA ACAAGTTCTA CAGGCCTGAG 
AACACCCACA AGTCCACTCC AGCGAGCTAC CACGCAGACA TCAACCTGCT CTACTGGAGC 
GACGAGGAGG CCGTGGTGGA CTTCAAGGCT GTGCAGGGCC GCTGCACCGT GGAGTATGGG 
GAGGACCTGC CCGAGTGCGT CCAGGTGTAC TCCATGGGCG GCCCCAACCG CTTCTACTTC 
CTCGAGGCCT ATAATGCAAA GAGCAAAAGC TTTGAAGATC CTCCCAACCA TGCCCGTAGC 
CCTGGAAACA AAGGGAAGGG CAAGGGAAAA GGGAAGGGCA AGCCCAAGTC CCAAGCCTGT 
GAGCCGAGCG AGCCAGAGAT AGAGATCAAG CTGCCCAAGC TGCGGACCCT GGATGTGTTT 
TCTGGCTGCG GGGGGTTGTC GGAGGGATTC CACCAAGCAG GCATCTCTGA CACGCTGTGG 
GCCATCGAGA TGTGGGACCC TGCGGCCCAG GCGTTCCGGC TGAACAACCC CGGCTCCACA 
GTGTTCACAG AGGACTGCAA CATCCTGCTG AAGCTGGTCA TGGCTGGGGA GACCACCAAC 
TCCCGCGGCC AGCGGCTGCC CCAGAAGGGA GACGTGGAGA TGCTGTGCGG CGGGCCGCCC 
TGCCAGGGCT TCAGCGGCAT GAACCGCTTC AATTCGCGCA CCTACTCCAA GTTCAAAAAC 
TCTCTGGTGG TTTCCTTCCT CAGCTACTGC GACTACTACC GGCCCCGGTT CTTCCTCCTG 
GAGAATGTCA GGAACTTTGT CTCCTTCAAG CGCTCCATGG TCCTGAAGCT CACCCTCCGC 
TGCCTGGTCC GCATGGGCTA TCAGTGCACC TTCGGCGTGC TGCAGGCCGG TCAGTACGGC 
GTGGCCCAGA CTAGGAGGCG GGCCATCATC CTGGCCGCGG CCCCTGGAGA GAAGCTCCCT 
CTGTTCCCGG AGCCACTGCA CGTGTTTGCT CCCCGGGCCT GCCAGCTGAG CGTGGTGGTG 
GATGACAAGA AGTTTGTGAG CAACATAACC AGGTTGAGCT CGGGTCCTTT CCGGACCATC 
ACGGTGCGAG ACACGATGTC CGACCTGCCG GAGGTGCGGA ATGGAGCCTC GGCACTGGAG 
ATCTCCTACA ACGGGGAGCC TCAGTCCTGG TTCCAGAGGC AGCTCCGGGG CGCACAGTAC 
CAGCCCATCC TCAGGGACCA CATCTGTAAG GACATGAGTG CATTGGTGGC TGCCCGCATG 
CGGCACATCC CCTTGGCCCC AGGGTCAGAC TGGCGCGATC TGCCCAACAT CGAGGTGCGG 
CTCTCAGACG GCACCATGGC CAGGAAGCTG CGGTATACCC ACCATGACAG GAAGAACGGC 
CGCAGCAGCT CTGGGGCCCT CCGTGGGGTC TGCTCCTGCG TGGAAGCCGG CAAAGCCTGC 
GACCCCGCAG CCAGGCAGTT CAACACCCTC ATCCCCTGGT GCCTGCCCCA CACCGGGAAC 
CGGCACAACC ACTGGGCTGG CCTCTATGGA AGGCTCGAGT GGGACGGCTT CTTCAGCACA 
ACCGTCACCA ACCCCGAGCC CATGGGCAAG CAGGGCCGCG TGCTCCACCC AGAGCAGCAC 
CGTGTGGTGA GCGTGCGGGA GTGTGCCCGC TCCCAGGGCT TCCCTGACAC CTACCGGCTC 
TTCGGCAACA TCCTGGACAA GCACCGGCAG GTGGGCAATG CCGTGCCACC GCCCCTGGCC 
AAAGCCATTG GCTTGGAGAT CAAGCTTTGT ATGTTGGCCA AAGCCCGAGA GAGTGCCTCA 
GCTAAAATAA AGGAGGAGGA AGCTGCTAAG GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNMT1 ( NM_001379.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001370.1 Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 2, mRNA.

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CloneID	OHu26697
Clone ID Related Accession (Same CDS sequence)	NM_001379.3 , NM_001379.4
Accession Version	NM_001379.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	DNA (cytosine-5)-methyltransferase 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X63692.1, BC126227.1, BC092517.1, DA653750.1 and HY245803.1. On Jan 9, 2016 this sequence version replaced NM_001379.2. Transcript Variant: This variant (2, also known as Dnmt1a in PMIDs 10449766 and 10753866) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting isoform (b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X63692.1, SRR1803617.79972.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:10721735) ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCCTGAA CCTTCACCTA GCCCCAGGAT TACAAGGAAA 
AGCACCAGGC AAACCACCAT CACATCTCAT TTTGCAAAGG GCCCTGCCAA ACGGAAACCT 
CAGGAAGAGT CTGAAAGAGC CAAATCGGAT GAGTCCATCA AGGAAGAAGA CAAAGACCAG 
GATGAGAAGA GACGTAGAGT TACATCCAGA GAACGAGTTG CTAGACCGCT TCCTGCAGAA 
GAACCTGAAA GAGCAAAATC AGGAACGCGC ACTGAAAAGG AAGAAGAAAG AGATGAAAAA 
GAAGAAAAGA GACTCCGAAG TCAAACCAAA GAACCAACAC CCAAACAGAA ACTGAAGGAG 
GAGCCGGACA GAGAAGCCAG GGCAGGCGTG CAGGCTGACG AGGACGAAGA TGGAGACGAG 
AAAGATGAGA AGAAGCACAG AAGTCAACCC AAAGATCTAG CTGCCAAACG GAGGCCCGAA 
GAAAAAGAAC CTGAAAAAGT AAATCCACAG ATTTCTGATG AAAAAGACGA GGATGAAAAG 
GAGGAGAAGA GACGCAAAAC GACCCCCAAA GAACCAACGG AGAAAAAAAT GGCTCGCGCC 
AAAACAGTCA TGAACTCCAA GACCCACCCT CCCAAGTGCA TTCAGTGCGG GCAGTACCTG 
GACGACCCTG ACCTCAAATA TGGGCAGCAC CCACCAGACG CGGTGGATGA GCCACAGATG 
CTGACAAATG AGAAGCTGTC CATCTTTGAT GCCAACGAGT CTGGCTTTGA GAGTTATGAG 
GCGCTTCCCC AGCACAAACT GACCTGCTTC AGTGTGTACT GTAAGCACGG TCACCTGTGT 
CCCATCGACA CCGGCCTCAT CGAGAAGAAT ATCGAACTCT TCTTTTCTGG TTCAGCAAAA 
CCAATCTATG ATGATGACCC ATCTCTTGAA GGTGGTGTTA ATGGCAAAAA TCTTGGCCCC 
ATAAATGAAT GGTGGATCAC TGGCTTTGAT GGAGGTGAAA AGGCCCTCAT CGGCTTCAGC 
ACCTCATTTG CCGAATACAT TCTGATGGAT CCCAGTCCCG AGTATGCGCC CATATTTGGG 
CTGATGCAGG AGAAGATCTA CATCAGCAAG ATTGTGGTGG AGTTCCTGCA GAGCAATTCC 
GACTCGACCT ATGAGGACCT GATCAACAAG ATCGAGACCA CGGTTCCTCC TTCTGGCCTC 
AACTTGAACC GCTTCACAGA GGACTCCCTC CTGCGACACG CGCAGTTTGT GGTGGAGCAG 
GTGGAGAGTT ATGACGAGGC CGGGGACAGT GATGAGCAGC CCATCTTCCT GACACCCTGC 
ATGCGGGACC TGATCAAGCT GGCTGGGGTC ACGCTGGGAC AGAGGCGAGC CCAGGCGAGG 
CGGCAGACCA TCAGGCATTC TACCAGGGAG AAGGACAGGG GACCCACGAA AGCCACCACC 
ACCAAGCTGG TCTACCAGAT CTTCGATACT TTCTTCGCAG AGCAAATTGA AAAGGATGAC 
AGAGAAGACA AGGAGAACGC CTTTAAGCGC CGGCGATGTG GCGTCTGTGA GGTGTGTCAG 
CAGCCTGAGT GTGGGAAATG TAAAGCCTGC AAGGACATGG TTAAATTTGG TGGCAGTGGA 
CGGAGCAAGC AGGCTTGCCA AGAGCGGAGG TGTCCCAATA TGGCCATGAA GGAGGCAGAT 
GACGATGAGG AAGTCGATGA TAACATCCCA GAGATGCCGT CACCCAAAAA AATGCACCAG 
GGGAAGAAGA AGAAACAGAA CAAGAATCGC ATCTCTTGGG TCGGAGAAGC CGTCAAGACT 
GATGGGAAGA AGAGTTACTA TAAGAAGGTG TGCATTGATG CGGAAACCCT GGAAGTGGGG 
GACTGTGTCT CTGTTATTCC AGATGATTCC TCAAAACCGC TGTATCTAGC AAGGGTCACG 
GCGCTGTGGG AGGACAGCAG CAACGGGCAG ATGTTTCACG CCCACTGGTT CTGCGCTGGG 
ACAGACACAG TCCTCGGGGC CACGTCGGAC CCTCTGGAGC TGTTCTTGGT GGATGAATGT 
GAGGACATGC AGCTTTCATA TATCCACAGC AAAGTGAAAG TCATCTACAA AGCCCCCTCC 
GAAAACTGGG CCATGGAGGG AGGCATGGAT CCCGAGTCCC TGCTGGAGGG GGACGACGGG 
AAGACCTACT TCTACCAGCT GTGGTATGAT CAAGACTACG CGAGATTCGA GTCCCCTCCA 
AAAACCCAGC CAACAGAGGA CAACAAGTTC AAATTCTGTG TGAGCTGTGC CCGTCTGGCT 
GAGATGAGGC AAAAAGAAAT CCCCAGGGTC CTGGAGCAGC TCGAGGACCT GGATAGCCGG 
GTCCTCTACT ACTCAGCCAC CAAGAACGGC ATCCTGTACC GAGTTGGTGA TGGTGTGTAC 
CTGCCCCCTG AGGCCTTCAC GTTCAACATC AAGCTGTCCA GTCCCGTGAA ACGCCCACGG 
AAGGAGCCCG TGGATGAGGA CCTGTACCCA GAGCACTACC GGAAATACTC CGACTACATC 
AAAGGCAGCA ACCTGGATGC CCCTGAGCCC TACCGAATTG GCCGGATCAA AGAGATCTTC 
TGTCCCAAGA AGAGCAACGG CAGGCCCAAT GAGACTGACA TCAAAATCCG GGTCAACAAG 
TTCTACAGGC CTGAGAACAC CCACAAGTCC ACTCCAGCGA GCTACCACGC AGACATCAAC 
CTGCTCTACT GGAGCGACGA GGAGGCCGTG GTGGACTTCA AGGCTGTGCA GGGCCGCTGC 
ACCGTGGAGT ATGGGGAGGA CCTGCCCGAG TGCGTCCAGG TGTACTCCAT GGGCGGCCCC 
AACCGCTTCT ACTTCCTCGA GGCCTATAAT GCAAAGAGCA AAAGCTTTGA AGATCCTCCC 
AACCATGCCC GTAGCCCTGG AAACAAAGGG AAGGGCAAGG GAAAAGGGAA GGGCAAGCCC 
AAGTCCCAAG CCTGTGAGCC GAGCGAGCCA GAGATAGAGA TCAAGCTGCC CAAGCTGCGG 
ACCCTGGATG TGTTTTCTGG CTGCGGGGGG TTGTCGGAGG GATTCCACCA AGCAGGCATC 
TCTGACACGC TGTGGGCCAT CGAGATGTGG GACCCTGCGG CCCAGGCGTT CCGGCTGAAC 
AACCCCGGCT CCACAGTGTT CACAGAGGAC TGCAACATCC TGCTGAAGCT GGTCATGGCT 
GGGGAGACCA CCAACTCCCG CGGCCAGCGG CTGCCCCAGA AGGGAGACGT GGAGATGCTG 
TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC GGCATGAACC GCTTCAATTC GCGCACCTAC 
TCCAAGTTCA AAAACTCTCT GGTGGTTTCC TTCCTCAGCT ACTGCGACTA CTACCGGCCC 
CGGTTCTTCC TCCTGGAGAA TGTCAGGAAC TTTGTCTCCT TCAAGCGCTC CATGGTCCTG 
AAGCTCACCC TCCGCTGCCT GGTCCGCATG GGCTATCAGT GCACCTTCGG CGTGCTGCAG 
GCCGGTCAGT ACGGCGTGGC CCAGACTAGG AGGCGGGCCA TCATCCTGGC CGCGGCCCCT 
GGAGAGAAGC TCCCTCTGTT CCCGGAGCCA CTGCACGTGT TTGCTCCCCG GGCCTGCCAG 
CTGAGCGTGG TGGTGGATGA CAAGAAGTTT GTGAGCAACA TAACCAGGTT GAGCTCGGGT 
CCTTTCCGGA CCATCACGGT GCGAGACACG ATGTCCGACC TGCCGGAGGT GCGGAATGGA 
GCCTCGGCAC TGGAGATCTC CTACAACGGG GAGCCTCAGT CCTGGTTCCA GAGGCAGCTC 
CGGGGCGCAC AGTACCAGCC CATCCTCAGG GACCACATCT GTAAGGACAT GAGTGCATTG 
GTGGCTGCCC GCATGCGGCA CATCCCCTTG GCCCCAGGGT CAGACTGGCG CGATCTGCCC 
AACATCGAGG TGCGGCTCTC AGACGGCACC ATGGCCAGGA AGCTGCGGTA TACCCACCAT 
GACAGGAAGA ACGGCCGCAG CAGCTCTGGG GCCCTCCGTG GGGTCTGCTC CTGCGTGGAA 
GCCGGCAAAG CCTGCGACCC CGCAGCCAGG CAGTTCAACA CCCTCATCCC CTGGTGCCTG 
CCCCACACCG GGAACCGGCA CAACCACTGG GCTGGCCTCT ATGGAAGGCT CGAGTGGGAC 
GGCTTCTTCA GCACAACCGT CACCAACCCC GAGCCCATGG GCAAGCAGGG CCGCGTGCTC 
CACCCAGAGC AGCACCGTGT GGTGAGCGTG CGGGAGTGTG CCCGCTCCCA GGGCTTCCCT 
GACACCTACC GGCTCTTCGG CAACATCCTG GACAAGCACC GGCAGGTGGG CAATGCCGTG 
CCACCGCCCC TGGCCAAAGC CATTGGCTTG GAGATCAAGC TTTGTATGTT GGCCAAAGCC 
CGAGAGAGTG CCTCAGCTAA AATAAAGGAG GAGGAAGCTG CTAAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001370.1
CDS	237..5087
Translation	MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

Target ORF information:

RefSeq Version	NM_001379.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001379.3

ORF Insert Sequence:

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCCTGAA CCTTCACCTA GCCCCAGGAT TACAAGGAAA 
AGCACCAGGC AAACCACCAT CACATCTCAT TTTGCAAAGG GCCCTGCCAA ACGGAAACCT 
CAGGAAGAGT CTGAAAGAGC CAAATCGGAT GAGTCCATCA AGGAAGAAGA CAAAGACCAG 
GATGAGAAGA GACGTAGAGT TACATCCAGA GAACGAGTTG CTAGACCGCT TCCTGCAGAA 
GAACCTGAAA GAGCAAAATC AGGAACGCGC ACTGAAAAGG AAGAAGAAAG AGATGAAAAA 
GAAGAAAAGA GACTCCGAAG TCAAACCAAA GAACCAACAC CCAAACAGAA ACTGAAGGAG 
GAGCCGGACA GAGAAGCCAG GGCAGGCGTG CAGGCTGACG AGGACGAAGA TGGAGACGAG 
AAAGATGAGA AGAAGCACAG AAGTCAACCC AAAGATCTAG CTGCCAAACG GAGGCCCGAA 
GAAAAAGAAC CTGAAAAAGT AAATCCACAG ATTTCTGATG AAAAAGACGA GGATGAAAAG 
GAGGAGAAGA GACGCAAAAC GACCCCCAAA GAACCAACGG AGAAAAAAAT GGCTCGCGCC 
AAAACAGTCA TGAACTCCAA GACCCACCCT CCCAAGTGCA TTCAGTGCGG GCAGTACCTG 
GACGACCCTG ACCTCAAATA TGGGCAGCAC CCACCAGACG CGGTGGATGA GCCACAGATG 
CTGACAAATG AGAAGCTGTC CATCTTTGAT GCCAACGAGT CTGGCTTTGA GAGTTATGAG 
GCGCTTCCCC AGCACAAACT GACCTGCTTC AGTGTGTACT GTAAGCACGG TCACCTGTGT 
CCCATCGACA CCGGCCTCAT CGAGAAGAAT ATCGAACTCT TCTTTTCTGG TTCAGCAAAA 
CCAATCTATG ATGATGACCC ATCTCTTGAA GGTGGTGTTA ATGGCAAAAA TCTTGGCCCC 
ATAAATGAAT GGTGGATCAC TGGCTTTGAT GGAGGTGAAA AGGCCCTCAT CGGCTTCAGC 
ACCTCATTTG CCGAATACAT TCTGATGGAT CCCAGTCCCG AGTATGCGCC CATATTTGGG 
CTGATGCAGG AGAAGATCTA CATCAGCAAG ATTGTGGTGG AGTTCCTGCA GAGCAATTCC 
GACTCGACCT ATGAGGACCT GATCAACAAG ATCGAGACCA CGGTTCCTCC TTCTGGCCTC 
AACTTGAACC GCTTCACAGA GGACTCCCTC CTGCGACACG CGCAGTTTGT GGTGGAGCAG 
GTGGAGAGTT ATGACGAGGC CGGGGACAGT GATGAGCAGC CCATCTTCCT GACACCCTGC 
ATGCGGGACC TGATCAAGCT GGCTGGGGTC ACGCTGGGAC AGAGGCGAGC CCAGGCGAGG 
CGGCAGACCA TCAGGCATTC TACCAGGGAG AAGGACAGGG GACCCACGAA AGCCACCACC 
ACCAAGCTGG TCTACCAGAT CTTCGATACT TTCTTCGCAG AGCAAATTGA AAAGGATGAC 
AGAGAAGACA AGGAGAACGC CTTTAAGCGC CGGCGATGTG GCGTCTGTGA GGTGTGTCAG 
CAGCCTGAGT GTGGGAAATG TAAAGCCTGC AAGGACATGG TTAAATTTGG TGGCAGTGGA 
CGGAGCAAGC AGGCTTGCCA AGAGCGGAGG TGTCCCAATA TGGCCATGAA GGAGGCAGAT 
GACGATGAGG AAGTCGATGA TAACATCCCA GAGATGCCGT CACCCAAAAA AATGCACCAG 
GGGAAGAAGA AGAAACAGAA CAAGAATCGC ATCTCTTGGG TCGGAGAAGC CGTCAAGACT 
GATGGGAAGA AGAGTTACTA TAAGAAGGTG TGCATTGATG CGGAAACCCT GGAAGTGGGG 
GACTGTGTCT CTGTTATTCC AGATGATTCC TCAAAACCGC TGTATCTAGC AAGGGTCACG 
GCGCTGTGGG AGGACAGCAG CAACGGGCAG ATGTTTCACG CCCACTGGTT CTGCGCTGGG 
ACAGACACAG TCCTCGGGGC CACGTCGGAC CCTCTGGAGC TGTTCTTGGT GGATGAATGT 
GAGGACATGC AGCTTTCATA TATCCACAGC AAAGTGAAAG TCATCTACAA AGCCCCCTCC 
GAAAACTGGG CCATGGAGGG AGGCATGGAT CCCGAGTCCC TGCTGGAGGG GGACGACGGG 
AAGACCTACT TCTACCAGCT GTGGTATGAT CAAGACTACG CGAGATTCGA GTCCCCTCCA 
AAAACCCAGC CAACAGAGGA CAACAAGTTC AAATTCTGTG TGAGCTGTGC CCGTCTGGCT 
GAGATGAGGC AAAAAGAAAT CCCCAGGGTC CTGGAGCAGC TCGAGGACCT GGATAGCCGG 
GTCCTCTACT ACTCAGCCAC CAAGAACGGC ATCCTGTACC GAGTTGGTGA TGGTGTGTAC 
CTGCCCCCTG AGGCCTTCAC GTTCAACATC AAGCTGTCCA GTCCCGTGAA ACGCCCACGG 
AAGGAGCCCG TGGATGAGGA CCTGTACCCA GAGCACTACC GGAAATACTC CGACTACATC 
AAAGGCAGCA ACCTGGATGC CCCTGAGCCC TACCGAATTG GCCGGATCAA AGAGATCTTC 
TGTCCCAAGA AGAGCAACGG CAGGCCCAAT GAGACTGACA TCAAAATCCG GGTCAACAAG 
TTCTACAGGC CTGAGAACAC CCACAAGTCC ACTCCAGCGA GCTACCACGC AGACATCAAC 
CTGCTCTACT GGAGCGACGA GGAGGCCGTG GTGGACTTCA AGGCTGTGCA GGGCCGCTGC 
ACCGTGGAGT ATGGGGAGGA CCTGCCCGAG TGCGTCCAGG TGTACTCCAT GGGCGGCCCC 
AACCGCTTCT ACTTCCTCGA GGCCTATAAT GCAAAGAGCA AAAGCTTTGA AGATCCTCCC 
AACCATGCCC GTAGCCCTGG AAACAAAGGG AAGGGCAAGG GAAAAGGGAA GGGCAAGCCC 
AAGTCCCAAG CCTGTGAGCC GAGCGAGCCA GAGATAGAGA TCAAGCTGCC CAAGCTGCGG 
ACCCTGGATG TGTTTTCTGG CTGCGGGGGG TTGTCGGAGG GATTCCACCA AGCAGGCATC 
TCTGACACGC TGTGGGCCAT CGAGATGTGG GACCCTGCGG CCCAGGCGTT CCGGCTGAAC 
AACCCCGGCT CCACAGTGTT CACAGAGGAC TGCAACATCC TGCTGAAGCT GGTCATGGCT 
GGGGAGACCA CCAACTCCCG CGGCCAGCGG CTGCCCCAGA AGGGAGACGT GGAGATGCTG 
TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC GGCATGAACC GCTTCAATTC GCGCACCTAC 
TCCAAGTTCA AAAACTCTCT GGTGGTTTCC TTCCTCAGCT ACTGCGACTA CTACCGGCCC 
CGGTTCTTCC TCCTGGAGAA TGTCAGGAAC TTTGTCTCCT TCAAGCGCTC CATGGTCCTG 
AAGCTCACCC TCCGCTGCCT GGTCCGCATG GGCTATCAGT GCACCTTCGG CGTGCTGCAG 
GCCGGTCAGT ACGGCGTGGC CCAGACTAGG AGGCGGGCCA TCATCCTGGC CGCGGCCCCT 
GGAGAGAAGC TCCCTCTGTT CCCGGAGCCA CTGCACGTGT TTGCTCCCCG GGCCTGCCAG 
CTGAGCGTGG TGGTGGATGA CAAGAAGTTT GTGAGCAACA TAACCAGGTT GAGCTCGGGT 
CCTTTCCGGA CCATCACGGT GCGAGACACG ATGTCCGACC TGCCGGAGGT GCGGAATGGA 
GCCTCGGCAC TGGAGATCTC CTACAACGGG GAGCCTCAGT CCTGGTTCCA GAGGCAGCTC 
CGGGGCGCAC AGTACCAGCC CATCCTCAGG GACCACATCT GTAAGGACAT GAGTGCATTG 
GTGGCTGCCC GCATGCGGCA CATCCCCTTG GCCCCAGGGT CAGACTGGCG CGATCTGCCC 
AACATCGAGG TGCGGCTCTC AGACGGCACC ATGGCCAGGA AGCTGCGGTA TACCCACCAT 
GACAGGAAGA ACGGCCGCAG CAGCTCTGGG GCCCTCCGTG GGGTCTGCTC CTGCGTGGAA 
GCCGGCAAAG CCTGCGACCC CGCAGCCAGG CAGTTCAACA CCCTCATCCC CTGGTGCCTG 
CCCCACACCG GGAACCGGCA CAACCACTGG GCTGGCCTCT ATGGAAGGCT CGAGTGGGAC 
GGCTTCTTCA GCACAACCGT CACCAACCCC GAGCCCATGG GCAAGCAGGG CCGCGTGCTC 
CACCCAGAGC AGCACCGTGT GGTGAGCGTG CGGGAGTGTG CCCGCTCCCA GGGCTTCCCT 
GACACCTACC GGCTCTTCGG CAACATCCTG GACAAGCACC GGCAGGTGGG CAATGCCGTG 
CCACCGCCCC TGGCCAAAGC CATTGGCTTG GAGATCAAGC TTTGTATGTT GGCCAAAGCC 
CGAGAGAGTG CCTCAGCTAA AATAAAGGAG GAGGAAGCTG CTAAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNMT1 ( NM_001379.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001370.1 Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 2, mRNA.

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CloneID	OHu26697
Clone ID Related Accession (Same CDS sequence)	NM_001379.3 , NM_001379.4
Accession Version	NM_001379.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	DNA (cytosine-5)-methyltransferase 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC126227.1, X63692.1, BC092517.1 and DA653750.1. On Jun 3, 2019 this sequence version replaced NM_001379.3. Transcript Variant: This variant (2, also known as Dnmt1a in PMIDs 10449766 and 10753866) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting isoform (b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.157645.1, SRR1803617.79972.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCCTGAA CCTTCACCTA GCCCCAGGAT TACAAGGAAA 
AGCACCAGGC AAACCACCAT CACATCTCAT TTTGCAAAGG GCCCTGCCAA ACGGAAACCT 
CAGGAAGAGT CTGAAAGAGC CAAATCGGAT GAGTCCATCA AGGAAGAAGA CAAAGACCAG 
GATGAGAAGA GACGTAGAGT TACATCCAGA GAACGAGTTG CTAGACCGCT TCCTGCAGAA 
GAACCTGAAA GAGCAAAATC AGGAACGCGC ACTGAAAAGG AAGAAGAAAG AGATGAAAAA 
GAAGAAAAGA GACTCCGAAG TCAAACCAAA GAACCAACAC CCAAACAGAA ACTGAAGGAG 
GAGCCGGACA GAGAAGCCAG GGCAGGCGTG CAGGCTGACG AGGACGAAGA TGGAGACGAG 
AAAGATGAGA AGAAGCACAG AAGTCAACCC AAAGATCTAG CTGCCAAACG GAGGCCCGAA 
GAAAAAGAAC CTGAAAAAGT AAATCCACAG ATTTCTGATG AAAAAGACGA GGATGAAAAG 
GAGGAGAAGA GACGCAAAAC GACCCCCAAA GAACCAACGG AGAAAAAAAT GGCTCGCGCC 
AAAACAGTCA TGAACTCCAA GACCCACCCT CCCAAGTGCA TTCAGTGCGG GCAGTACCTG 
GACGACCCTG ACCTCAAATA TGGGCAGCAC CCACCAGACG CGGTGGATGA GCCACAGATG 
CTGACAAATG AGAAGCTGTC CATCTTTGAT GCCAACGAGT CTGGCTTTGA GAGTTATGAG 
GCGCTTCCCC AGCACAAACT GACCTGCTTC AGTGTGTACT GTAAGCACGG TCACCTGTGT 
CCCATCGACA CCGGCCTCAT CGAGAAGAAT ATCGAACTCT TCTTTTCTGG TTCAGCAAAA 
CCAATCTATG ATGATGACCC ATCTCTTGAA GGTGGTGTTA ATGGCAAAAA TCTTGGCCCC 
ATAAATGAAT GGTGGATCAC TGGCTTTGAT GGAGGTGAAA AGGCCCTCAT CGGCTTCAGC 
ACCTCATTTG CCGAATACAT TCTGATGGAT CCCAGTCCCG AGTATGCGCC CATATTTGGG 
CTGATGCAGG AGAAGATCTA CATCAGCAAG ATTGTGGTGG AGTTCCTGCA GAGCAATTCC 
GACTCGACCT ATGAGGACCT GATCAACAAG ATCGAGACCA CGGTTCCTCC TTCTGGCCTC 
AACTTGAACC GCTTCACAGA GGACTCCCTC CTGCGACACG CGCAGTTTGT GGTGGAGCAG 
GTGGAGAGTT ATGACGAGGC CGGGGACAGT GATGAGCAGC CCATCTTCCT GACACCCTGC 
ATGCGGGACC TGATCAAGCT GGCTGGGGTC ACGCTGGGAC AGAGGCGAGC CCAGGCGAGG 
CGGCAGACCA TCAGGCATTC TACCAGGGAG AAGGACAGGG GACCCACGAA AGCCACCACC 
ACCAAGCTGG TCTACCAGAT CTTCGATACT TTCTTCGCAG AGCAAATTGA AAAGGATGAC 
AGAGAAGACA AGGAGAACGC CTTTAAGCGC CGGCGATGTG GCGTCTGTGA GGTGTGTCAG 
CAGCCTGAGT GTGGGAAATG TAAAGCCTGC AAGGACATGG TTAAATTTGG TGGCAGTGGA 
CGGAGCAAGC AGGCTTGCCA AGAGCGGAGG TGTCCCAATA TGGCCATGAA GGAGGCAGAT 
GACGATGAGG AAGTCGATGA TAACATCCCA GAGATGCCGT CACCCAAAAA AATGCACCAG 
GGGAAGAAGA AGAAACAGAA CAAGAATCGC ATCTCTTGGG TCGGAGAAGC CGTCAAGACT 
GATGGGAAGA AGAGTTACTA TAAGAAGGTG TGCATTGATG CGGAAACCCT GGAAGTGGGG 
GACTGTGTCT CTGTTATTCC AGATGATTCC TCAAAACCGC TGTATCTAGC AAGGGTCACG 
GCGCTGTGGG AGGACAGCAG CAACGGGCAG ATGTTTCACG CCCACTGGTT CTGCGCTGGG 
ACAGACACAG TCCTCGGGGC CACGTCGGAC CCTCTGGAGC TGTTCTTGGT GGATGAATGT 
GAGGACATGC AGCTTTCATA TATCCACAGC AAAGTGAAAG TCATCTACAA AGCCCCCTCC 
GAAAACTGGG CCATGGAGGG AGGCATGGAT CCCGAGTCCC TGCTGGAGGG GGACGACGGG 
AAGACCTACT TCTACCAGCT GTGGTATGAT CAAGACTACG CGAGATTCGA GTCCCCTCCA 
AAAACCCAGC CAACAGAGGA CAACAAGTTC AAATTCTGTG TGAGCTGTGC CCGTCTGGCT 
GAGATGAGGC AAAAAGAAAT CCCCAGGGTC CTGGAGCAGC TCGAGGACCT GGATAGCCGG 
GTCCTCTACT ACTCAGCCAC CAAGAACGGC ATCCTGTACC GAGTTGGTGA TGGTGTGTAC 
CTGCCCCCTG AGGCCTTCAC GTTCAACATC AAGCTGTCCA GTCCCGTGAA ACGCCCACGG 
AAGGAGCCCG TGGATGAGGA CCTGTACCCA GAGCACTACC GGAAATACTC CGACTACATC 
AAAGGCAGCA ACCTGGATGC CCCTGAGCCC TACCGAATTG GCCGGATCAA AGAGATCTTC 
TGTCCCAAGA AGAGCAACGG CAGGCCCAAT GAGACTGACA TCAAAATCCG GGTCAACAAG 
TTCTACAGGC CTGAGAACAC CCACAAGTCC ACTCCAGCGA GCTACCACGC AGACATCAAC 
CTGCTCTACT GGAGCGACGA GGAGGCCGTG GTGGACTTCA AGGCTGTGCA GGGCCGCTGC 
ACCGTGGAGT ATGGGGAGGA CCTGCCCGAG TGCGTCCAGG TGTACTCCAT GGGCGGCCCC 
AACCGCTTCT ACTTCCTCGA GGCCTATAAT GCAAAGAGCA AAAGCTTTGA AGATCCTCCC 
AACCATGCCC GTAGCCCTGG AAACAAAGGG AAGGGCAAGG GAAAAGGGAA GGGCAAGCCC 
AAGTCCCAAG CCTGTGAGCC GAGCGAGCCA GAGATAGAGA TCAAGCTGCC CAAGCTGCGG 
ACCCTGGATG TGTTTTCTGG CTGCGGGGGG TTGTCGGAGG GATTCCACCA AGCAGGCATC 
TCTGACACGC TGTGGGCCAT CGAGATGTGG GACCCTGCGG CCCAGGCGTT CCGGCTGAAC 
AACCCCGGCT CCACAGTGTT CACAGAGGAC TGCAACATCC TGCTGAAGCT GGTCATGGCT 
GGGGAGACCA CCAACTCCCG CGGCCAGCGG CTGCCCCAGA AGGGAGACGT GGAGATGCTG 
TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC GGCATGAACC GCTTCAATTC GCGCACCTAC 
TCCAAGTTCA AAAACTCTCT GGTGGTTTCC TTCCTCAGCT ACTGCGACTA CTACCGGCCC 
CGGTTCTTCC TCCTGGAGAA TGTCAGGAAC TTTGTCTCCT TCAAGCGCTC CATGGTCCTG 
AAGCTCACCC TCCGCTGCCT GGTCCGCATG GGCTATCAGT GCACCTTCGG CGTGCTGCAG 
GCCGGTCAGT ACGGCGTGGC CCAGACTAGG AGGCGGGCCA TCATCCTGGC CGCGGCCCCT 
GGAGAGAAGC TCCCTCTGTT CCCGGAGCCA CTGCACGTGT TTGCTCCCCG GGCCTGCCAG 
CTGAGCGTGG TGGTGGATGA CAAGAAGTTT GTGAGCAACA TAACCAGGTT GAGCTCGGGT 
CCTTTCCGGA CCATCACGGT GCGAGACACG ATGTCCGACC TGCCGGAGGT GCGGAATGGA 
GCCTCGGCAC TGGAGATCTC CTACAACGGG GAGCCTCAGT CCTGGTTCCA GAGGCAGCTC 
CGGGGCGCAC AGTACCAGCC CATCCTCAGG GACCACATCT GTAAGGACAT GAGTGCATTG 
GTGGCTGCCC GCATGCGGCA CATCCCCTTG GCCCCAGGGT CAGACTGGCG CGATCTGCCC 
AACATCGAGG TGCGGCTCTC AGACGGCACC ATGGCCAGGA AGCTGCGGTA TACCCACCAT 
GACAGGAAGA ACGGCCGCAG CAGCTCTGGG GCCCTCCGTG GGGTCTGCTC CTGCGTGGAA 
GCCGGCAAAG CCTGCGACCC CGCAGCCAGG CAGTTCAACA CCCTCATCCC CTGGTGCCTG 
CCCCACACCG GGAACCGGCA CAACCACTGG GCTGGCCTCT ATGGAAGGCT CGAGTGGGAC 
GGCTTCTTCA GCACAACCGT CACCAACCCC GAGCCCATGG GCAAGCAGGG CCGCGTGCTC 
CACCCAGAGC AGCACCGTGT GGTGAGCGTG CGGGAGTGTG CCCGCTCCCA GGGCTTCCCT 
GACACCTACC GGCTCTTCGG CAACATCCTG GACAAGCACC GGCAGGTGGG CAATGCCGTG 
CCACCGCCCC TGGCCAAAGC CATTGGCTTG GAGATCAAGC TTTGTATGTT GGCCAAAGCC 
CGAGAGAGTG CCTCAGCTAA AATAAAGGAG GAGGAAGCTG CTAAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001370.1
CDS	55..4905
Translation	MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

Target ORF information:

RefSeq Version	NM_001379.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001379.4

ORF Insert Sequence:

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCCTGAA CCTTCACCTA GCCCCAGGAT TACAAGGAAA 
AGCACCAGGC AAACCACCAT CACATCTCAT TTTGCAAAGG GCCCTGCCAA ACGGAAACCT 
CAGGAAGAGT CTGAAAGAGC CAAATCGGAT GAGTCCATCA AGGAAGAAGA CAAAGACCAG 
GATGAGAAGA GACGTAGAGT TACATCCAGA GAACGAGTTG CTAGACCGCT TCCTGCAGAA 
GAACCTGAAA GAGCAAAATC AGGAACGCGC ACTGAAAAGG AAGAAGAAAG AGATGAAAAA 
GAAGAAAAGA GACTCCGAAG TCAAACCAAA GAACCAACAC CCAAACAGAA ACTGAAGGAG 
GAGCCGGACA GAGAAGCCAG GGCAGGCGTG CAGGCTGACG AGGACGAAGA TGGAGACGAG 
AAAGATGAGA AGAAGCACAG AAGTCAACCC AAAGATCTAG CTGCCAAACG GAGGCCCGAA 
GAAAAAGAAC CTGAAAAAGT AAATCCACAG ATTTCTGATG AAAAAGACGA GGATGAAAAG 
GAGGAGAAGA GACGCAAAAC GACCCCCAAA GAACCAACGG AGAAAAAAAT GGCTCGCGCC 
AAAACAGTCA TGAACTCCAA GACCCACCCT CCCAAGTGCA TTCAGTGCGG GCAGTACCTG 
GACGACCCTG ACCTCAAATA TGGGCAGCAC CCACCAGACG CGGTGGATGA GCCACAGATG 
CTGACAAATG AGAAGCTGTC CATCTTTGAT GCCAACGAGT CTGGCTTTGA GAGTTATGAG 
GCGCTTCCCC AGCACAAACT GACCTGCTTC AGTGTGTACT GTAAGCACGG TCACCTGTGT 
CCCATCGACA CCGGCCTCAT CGAGAAGAAT ATCGAACTCT TCTTTTCTGG TTCAGCAAAA 
CCAATCTATG ATGATGACCC ATCTCTTGAA GGTGGTGTTA ATGGCAAAAA TCTTGGCCCC 
ATAAATGAAT GGTGGATCAC TGGCTTTGAT GGAGGTGAAA AGGCCCTCAT CGGCTTCAGC 
ACCTCATTTG CCGAATACAT TCTGATGGAT CCCAGTCCCG AGTATGCGCC CATATTTGGG 
CTGATGCAGG AGAAGATCTA CATCAGCAAG ATTGTGGTGG AGTTCCTGCA GAGCAATTCC 
GACTCGACCT ATGAGGACCT GATCAACAAG ATCGAGACCA CGGTTCCTCC TTCTGGCCTC 
AACTTGAACC GCTTCACAGA GGACTCCCTC CTGCGACACG CGCAGTTTGT GGTGGAGCAG 
GTGGAGAGTT ATGACGAGGC CGGGGACAGT GATGAGCAGC CCATCTTCCT GACACCCTGC 
ATGCGGGACC TGATCAAGCT GGCTGGGGTC ACGCTGGGAC AGAGGCGAGC CCAGGCGAGG 
CGGCAGACCA TCAGGCATTC TACCAGGGAG AAGGACAGGG GACCCACGAA AGCCACCACC 
ACCAAGCTGG TCTACCAGAT CTTCGATACT TTCTTCGCAG AGCAAATTGA AAAGGATGAC 
AGAGAAGACA AGGAGAACGC CTTTAAGCGC CGGCGATGTG GCGTCTGTGA GGTGTGTCAG 
CAGCCTGAGT GTGGGAAATG TAAAGCCTGC AAGGACATGG TTAAATTTGG TGGCAGTGGA 
CGGAGCAAGC AGGCTTGCCA AGAGCGGAGG TGTCCCAATA TGGCCATGAA GGAGGCAGAT 
GACGATGAGG AAGTCGATGA TAACATCCCA GAGATGCCGT CACCCAAAAA AATGCACCAG 
GGGAAGAAGA AGAAACAGAA CAAGAATCGC ATCTCTTGGG TCGGAGAAGC CGTCAAGACT 
GATGGGAAGA AGAGTTACTA TAAGAAGGTG TGCATTGATG CGGAAACCCT GGAAGTGGGG 
GACTGTGTCT CTGTTATTCC AGATGATTCC TCAAAACCGC TGTATCTAGC AAGGGTCACG 
GCGCTGTGGG AGGACAGCAG CAACGGGCAG ATGTTTCACG CCCACTGGTT CTGCGCTGGG 
ACAGACACAG TCCTCGGGGC CACGTCGGAC CCTCTGGAGC TGTTCTTGGT GGATGAATGT 
GAGGACATGC AGCTTTCATA TATCCACAGC AAAGTGAAAG TCATCTACAA AGCCCCCTCC 
GAAAACTGGG CCATGGAGGG AGGCATGGAT CCCGAGTCCC TGCTGGAGGG GGACGACGGG 
AAGACCTACT TCTACCAGCT GTGGTATGAT CAAGACTACG CGAGATTCGA GTCCCCTCCA 
AAAACCCAGC CAACAGAGGA CAACAAGTTC AAATTCTGTG TGAGCTGTGC CCGTCTGGCT 
GAGATGAGGC AAAAAGAAAT CCCCAGGGTC CTGGAGCAGC TCGAGGACCT GGATAGCCGG 
GTCCTCTACT ACTCAGCCAC CAAGAACGGC ATCCTGTACC GAGTTGGTGA TGGTGTGTAC 
CTGCCCCCTG AGGCCTTCAC GTTCAACATC AAGCTGTCCA GTCCCGTGAA ACGCCCACGG 
AAGGAGCCCG TGGATGAGGA CCTGTACCCA GAGCACTACC GGAAATACTC CGACTACATC 
AAAGGCAGCA ACCTGGATGC CCCTGAGCCC TACCGAATTG GCCGGATCAA AGAGATCTTC 
TGTCCCAAGA AGAGCAACGG CAGGCCCAAT GAGACTGACA TCAAAATCCG GGTCAACAAG 
TTCTACAGGC CTGAGAACAC CCACAAGTCC ACTCCAGCGA GCTACCACGC AGACATCAAC 
CTGCTCTACT GGAGCGACGA GGAGGCCGTG GTGGACTTCA AGGCTGTGCA GGGCCGCTGC 
ACCGTGGAGT ATGGGGAGGA CCTGCCCGAG TGCGTCCAGG TGTACTCCAT GGGCGGCCCC 
AACCGCTTCT ACTTCCTCGA GGCCTATAAT GCAAAGAGCA AAAGCTTTGA AGATCCTCCC 
AACCATGCCC GTAGCCCTGG AAACAAAGGG AAGGGCAAGG GAAAAGGGAA GGGCAAGCCC 
AAGTCCCAAG CCTGTGAGCC GAGCGAGCCA GAGATAGAGA TCAAGCTGCC CAAGCTGCGG 
ACCCTGGATG TGTTTTCTGG CTGCGGGGGG TTGTCGGAGG GATTCCACCA AGCAGGCATC 
TCTGACACGC TGTGGGCCAT CGAGATGTGG GACCCTGCGG CCCAGGCGTT CCGGCTGAAC 
AACCCCGGCT CCACAGTGTT CACAGAGGAC TGCAACATCC TGCTGAAGCT GGTCATGGCT 
GGGGAGACCA CCAACTCCCG CGGCCAGCGG CTGCCCCAGA AGGGAGACGT GGAGATGCTG 
TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC GGCATGAACC GCTTCAATTC GCGCACCTAC 
TCCAAGTTCA AAAACTCTCT GGTGGTTTCC TTCCTCAGCT ACTGCGACTA CTACCGGCCC 
CGGTTCTTCC TCCTGGAGAA TGTCAGGAAC TTTGTCTCCT TCAAGCGCTC CATGGTCCTG 
AAGCTCACCC TCCGCTGCCT GGTCCGCATG GGCTATCAGT GCACCTTCGG CGTGCTGCAG 
GCCGGTCAGT ACGGCGTGGC CCAGACTAGG AGGCGGGCCA TCATCCTGGC CGCGGCCCCT 
GGAGAGAAGC TCCCTCTGTT CCCGGAGCCA CTGCACGTGT TTGCTCCCCG GGCCTGCCAG 
CTGAGCGTGG TGGTGGATGA CAAGAAGTTT GTGAGCAACA TAACCAGGTT GAGCTCGGGT 
CCTTTCCGGA CCATCACGGT GCGAGACACG ATGTCCGACC TGCCGGAGGT GCGGAATGGA 
GCCTCGGCAC TGGAGATCTC CTACAACGGG GAGCCTCAGT CCTGGTTCCA GAGGCAGCTC 
CGGGGCGCAC AGTACCAGCC CATCCTCAGG GACCACATCT GTAAGGACAT GAGTGCATTG 
GTGGCTGCCC GCATGCGGCA CATCCCCTTG GCCCCAGGGT CAGACTGGCG CGATCTGCCC 
AACATCGAGG TGCGGCTCTC AGACGGCACC ATGGCCAGGA AGCTGCGGTA TACCCACCAT 
GACAGGAAGA ACGGCCGCAG CAGCTCTGGG GCCCTCCGTG GGGTCTGCTC CTGCGTGGAA 
GCCGGCAAAG CCTGCGACCC CGCAGCCAGG CAGTTCAACA CCCTCATCCC CTGGTGCCTG 
CCCCACACCG GGAACCGGCA CAACCACTGG GCTGGCCTCT ATGGAAGGCT CGAGTGGGAC 
GGCTTCTTCA GCACAACCGT CACCAACCCC GAGCCCATGG GCAAGCAGGG CCGCGTGCTC 
CACCCAGAGC AGCACCGTGT GGTGAGCGTG CGGGAGTGTG CCCGCTCCCA GGGCTTCCCT 
GACACCTACC GGCTCTTCGG CAACATCCTG GACAAGCACC GGCAGGTGGG CAATGCCGTG 
CCACCGCCCC TGGCCAAAGC CATTGGCTTG GAGATCAAGC TTTGTATGTT GGCCAAAGCC 
CGAGAGAGTG CCTCAGCTAA AATAAAGGAG GAGGAAGCTG CTAAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNMT1 ( NM_001318730.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305659.1 Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu57334
Clone ID Related Accession (Same CDS sequence)	NM_001318730.2 , NM_001318730.1
Accession Version	NM_001318730.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	DNA (cytosine-5)-methyltransferase 1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX379258.2, BC126227.1, X63692.1, BC092517.1, DA653750.1, AB209413.2 and HY245803.1. On May 31, 2019 this sequence version replaced NM_001318730.1. Transcript Variant: This variant (3) has a shorter 5' UTR, lacks an alternate in-frame exon in the 5' coding region, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209413.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCCTGAA CCTTCACCTA GCCCCAGGAT TACAAGGAAA 
AGCACCAGGC AAACCACCAT CACATCTCAT TTTGCAAAGG GCCCTGCCAA ACGGAAACCT 
CAGGAAGAGT CTGAAAGAGC CAAATCGGAT GAGTCCATCA AGGAAGAAGA CAAAGACCAG 
GATGAGAAGA GACGTAGAGT TACATCCAGA GAACGAGTTG CTAGACCGCT TCCTGCAGAA 
GAACCTGAAA GAGCAAAATC AGGAACGCGC ACTGAAAAGG AAGAAGAAAG AGATGAAAAA 
GAAGAAAAGA GACTCCGAAG TCAAACCAAA GAACCAACAC CCAAACAGAA ACTGAAGGAG 
GAGCCGGACA GAGAAGCCAG GGCAGGCGTG CAGGCTGACG AGGACGAAGA TGGAGACGAG 
AAAGATGAGA AGAAGCACAG AAGTCAACCC AAAGATCTAG CTGCCAAACG GAGGCCCGAA 
GAAAAAGAAC CTGAAAAAGT AAATCCACAG ATTTCTGATG AAAAAGACGA GGATGAAAAG 
GAGGAGAAGA GACGCAAAAC GACCCCCAAA GAACCAACGG AGAAAAAAAT GGCTCGCGCC 
AAAACAGTCA TGAACTCCAA GACCCACCCT CCCAAGTGCA TTCAGTGCGG GCAGTACCTG 
GACGACCCTG ACCTCAAATA TGGGCAGCAC CCACCAGACG CGGTGGATGA GCCACAGATG 
CTGACAAATG AGAAGCTGTC CATCTTTGAT GCCAACGAGT CTGGCTTTGA GAGTTATGAG 
GCGCTTCCCC AGCACAAACT GACCTGCTTC AGTGTGTACT GTAAGCACGG TCACCTGTGT 
CCCATCGACA CCGGCCTCAT CGAGAAGAAT ATCGAACTCT TCTTTTCTGG TTCAGCAAAA 
CCAATCTATG ATGATGACCC ATCTCTTGAA GGTGGTGTTA ATGGCAAAAA TCTTGGCCCC 
ATAAATGAAT GGTGGATCAC TGGCTTTGAT GGAGGTGAAA AGGCCCTCAT CGGCTTCAGC 
ACCTCATTTG CCGAATACAT TCTGATGGAT CCCAGTCCCG AGTATGCGCC CATATTTGGG 
CTGATGCAGG AGAAGATCTA CATCAGCAAG ATTGTGGTGG AGTTCCTGCA GAGCAATTCC 
GACTCGACCT ATGAGGACCT GATCAACAAG ATCGAGACCA CGGTTCCTCC TTCTGGCCTC 
AACTTGAACC GCTTCACAGA GGACTCCCTC CTGCGACACG CGCAGTTTGT GGTGGAGCAG 
GTGGAGAGTT ATGACGAGGC CGGGGACAGT GATGAGCAGC CCATCTTCCT GACACCCTGC 
ATGCGGGACC TGATCAAGCT GGCTGGGGTC ACGCTGGGAC AGAGGCGAGC CCAGGCGAGG 
CGGCAGACCA TCAGGCATTC TACCAGGGAG AAGGACAGGG GACCCACGAA AGCCACCACC 
ACCAAGCTGG TCTACCAGAT CTTCGATACT TTCTTCGCAG AGCAAATTGA AAAGGATGAC 
AGAGAAGACA AGGAGAACGC CTTTAAGCGC CGGCGATGTG GCGTCTGTGA GGTGTGTCAG 
CAGCCTGAGT GTGGGAAATG TAAAGCCTGC AAGGACATGG TTAAATTTGG TGGCAGTGGA 
CGGAGCAAGC AGGCTTGCCA AGAGCGGAGG TGTCCCAATA TGGCCATGAA GGAGGCAGAT 
GACGATGAGG AAGTCGATGA TAACATCCCA GAGATGCCGT CACCCAAAAA AATGCACCAG 
GGGAAGAAGA AGAAACAGAA CAAGAATCGC ATCTCTTGGG TCGGAGAAGC CGTCAAGACT 
GATGGGAAGA AGAGTTACTA TAAGAAGGTG TGCATTGATG CGGAAACCCT GGAAGTGGGG 
GACTGTGTCT CTGTTATTCC AGATGATTCC TCAAAACCGC TGTATCTAGC AAGGGTCACG 
GCGCTGTGGG AGGACAGCAG CAACGGGCAG ATGTTTCACG CCCACTGGTT CTGCGCTGGG 
ACAGACACAG TCCTCGGGGC CACGTCGGAC CCTCTGGAGC TGTTCTTGGT GGATGAATGT 
GAGGACATGC AGCTTTCATA TATCCACAGC AAAGTGAAAG TCATCTACAA AGCCCCCTCC 
GAAAACTGGG CCATGGAGGG AGGCATGGAT CCCGAGTCCC TGCTGGAGGG GGACGACGGG 
AAGACCTACT TCTACCAGCT GTGGTATGAT CAAGACTACG CGAGATTCGA GTCCCCTCCA 
AAAACCCAGC CAACAGAGGA CAACAAGTTC AAATTCTGTG TGAGCTGTGC CCGTCTGGCT 
GAGATGAGGC AAAAAGAAAT CCCCAGGGTC CTGGAGCAGC TCGAGGACCT GGATAGCCGG 
GTCCTCTACT ACTCAGCCAC CAAGAACGGC ATCCTGTACC GAGTTGGTGA TGGTGTGTAC 
CTGCCCCCTG AGGCCTTCAC GTTCAACATC AAGCTGTCCA GTCCCGTGAA ACGCCCACGG 
AAGGAGCCCG TGGATGAGGA CCTGTACCCA GAGCACTACC GGAAATACTC CGACTACATC 
AAAGGCAGCA ACCTGGATGC CCCTGAGCCC TACCGAATTG GCCGGATCAA AGAGATCTTC 
TGTCCCAAGA AGAGCAACGG CAGGCCCAAT GAGACTGACA TCAAAATCCG GGTCAACAAG 
TTCTACAGGC CTGAGAACAC CCACAAGTCC ACTCCAGCGA GCTACCACGC AGACATCAAC 
CTGCTCTACT GGAGCGACGA GGAGGCCGTG GTGGACTTCA AGGCTGTGCA GGGCCGCTGC 
ACCGTGGAGT ATGGGGAGGA CCTGCCCGAG TGCGTCCAGG TGTACTCCAT GGGCGGCCCC 
AACCGCTTCT ACTTCCTCGA GGCCTATAAT GCAAAGAGCA AAAGCTTTGA AGATCCTCCC 
AACCATGCCC GTAGCCCTGG AAACAAAGGG AAGGGCAAGG GAAAAGGGAA GGGCAAGCCC 
AAGTCCCAAG CCTGTGAGCC GAGCGAGCCA GAGATAGAGA TCAAGCTGCC CAAGCTGCGG 
ACCCTGGATG TGTTTTCTGG CTGCGGGGGG TTGTCGGAGG GATTCCACCA AGCAGGCATC 
TCTGACACGC TGTGGGCCAT CGAGATGTGG GACCCTGCGG CCCAGGCGTT CCGGCTGAAC 
AACCCCGGCT CCACAGTGTT CACAGAGGAC TGCAACATCC TGCTGAAGCT GGTCATGGCT 
GGGGAGACCA CCAACTCCCG CGGCCAGCGG CTGCCCCAGA AGGGAGACGT GGAGATGCTG 
TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC GGCATGAACC GCTTCAATTC GCGCACCTAC 
TCCAAGTTCA AAAACTCTCT GGTGGTTTCC TTCCTCAGCT ACTGCGACTA CTACCGGCCC 
CGGTTCTTCC TCCTGGAGAA TGTCAGGAAC TTTGTCTCCT TCAAGCGCTC CATGGTCCTG 
AAGCTCACCC TCCGCTGCCT GGTCCGCATG GGCTATCAGT GCACCTTCGG CGTGCTGCAG 
GCCGGTCAGT ACGGCGTGGC CCAGACTAGG AGGCGGGCCA TCATCCTGGC CGCGGCCCCT 
GGAGAGAAGC TCCCTCTGTT CCCGGAGCCA CTGCACGTGT TTGCTCCCCG GGCCTGCCAG 
CTGAGCGTGG TGGTGGATGA CAAGAAGTTT GTGAGCAACA TAACCAGGTT GAGCTCGGGT 
CCTTTCCGGA CCATCACGGT GCGAGACACG ATGTCCGACC TGCCGGAGGT GCGGAATGGA 
GCCTCGGCAC TGGAGATCTC CTACAACGGG GAGCCTCAGT CCTGGTTCCA GAGGCAGCTC 
CGGGGCGCAC AGTACCAGCC CATCCTCAGG GACCACATCT GTAAGGACAT GAGTGCATTG 
GTGGCTGCCC GCATGCGGCA CATCCCCTTG GCCCCAGGGT CAGACTGGCG CGATCTGCCC 
AACATCGAGG TGCGGCTCTC AGACGGCACC ATGGCCAGGA AGCTGCGGTA TACCCACCAT 
GACAGGAAGA ACGGCCGCAG CAGCTCTGGG GCCCTCCGTG GGGTCTGCTC CTGCGTGGAA 
GGTGGGTCCT CCGGCAAAGC CTGCGACCCC GCAGCCAGGC AGTTCAACAC CCTCATCCCC 
TGGTGCCTGC CCCACACCGG GAACCGGCAC AACCACTGGG CTGGCCTCTA TGGAAGGCTC 
GAGTGGGACG GCTTCTTCAG CACAACCGTC ACCAACCCCG AGCCCATGGG CAAGCAGGGC 
CGCGTGCTCC ACCCAGAGCA GCACCGTGTG GTGAGCGTGC GGGAGTGTGC CCGCTCCCAG 
GGCTTCCCTG ACACCTACCG GCTCTTCGGC AACATCCTGG ACAAGCACCG GCAGGTGGGC 
AATGCCGTGC CACCGCCCCT GGCCAAAGCC ATTGGCTTGG AGATCAAGCT TTGTATGTTG 
GCCAAAGCCC GAGAGAGTGC CTCAGCTAAA ATAAAGGAGG AGGAAGCTGC TAAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305659.1
CDS	55..4914
Translation	MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEGGSSGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

Target ORF information:

RefSeq Version	NM_001318730.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318730.2

ORF Insert Sequence:

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCCTGAA CCTTCACCTA GCCCCAGGAT TACAAGGAAA 
AGCACCAGGC AAACCACCAT CACATCTCAT TTTGCAAAGG GCCCTGCCAA ACGGAAACCT 
CAGGAAGAGT CTGAAAGAGC CAAATCGGAT GAGTCCATCA AGGAAGAAGA CAAAGACCAG 
GATGAGAAGA GACGTAGAGT TACATCCAGA GAACGAGTTG CTAGACCGCT TCCTGCAGAA 
GAACCTGAAA GAGCAAAATC AGGAACGCGC ACTGAAAAGG AAGAAGAAAG AGATGAAAAA 
GAAGAAAAGA GACTCCGAAG TCAAACCAAA GAACCAACAC CCAAACAGAA ACTGAAGGAG 
GAGCCGGACA GAGAAGCCAG GGCAGGCGTG CAGGCTGACG AGGACGAAGA TGGAGACGAG 
AAAGATGAGA AGAAGCACAG AAGTCAACCC AAAGATCTAG CTGCCAAACG GAGGCCCGAA 
GAAAAAGAAC CTGAAAAAGT AAATCCACAG ATTTCTGATG AAAAAGACGA GGATGAAAAG 
GAGGAGAAGA GACGCAAAAC GACCCCCAAA GAACCAACGG AGAAAAAAAT GGCTCGCGCC 
AAAACAGTCA TGAACTCCAA GACCCACCCT CCCAAGTGCA TTCAGTGCGG GCAGTACCTG 
GACGACCCTG ACCTCAAATA TGGGCAGCAC CCACCAGACG CGGTGGATGA GCCACAGATG 
CTGACAAATG AGAAGCTGTC CATCTTTGAT GCCAACGAGT CTGGCTTTGA GAGTTATGAG 
GCGCTTCCCC AGCACAAACT GACCTGCTTC AGTGTGTACT GTAAGCACGG TCACCTGTGT 
CCCATCGACA CCGGCCTCAT CGAGAAGAAT ATCGAACTCT TCTTTTCTGG TTCAGCAAAA 
CCAATCTATG ATGATGACCC ATCTCTTGAA GGTGGTGTTA ATGGCAAAAA TCTTGGCCCC 
ATAAATGAAT GGTGGATCAC TGGCTTTGAT GGAGGTGAAA AGGCCCTCAT CGGCTTCAGC 
ACCTCATTTG CCGAATACAT TCTGATGGAT CCCAGTCCCG AGTATGCGCC CATATTTGGG 
CTGATGCAGG AGAAGATCTA CATCAGCAAG ATTGTGGTGG AGTTCCTGCA GAGCAATTCC 
GACTCGACCT ATGAGGACCT GATCAACAAG ATCGAGACCA CGGTTCCTCC TTCTGGCCTC 
AACTTGAACC GCTTCACAGA GGACTCCCTC CTGCGACACG CGCAGTTTGT GGTGGAGCAG 
GTGGAGAGTT ATGACGAGGC CGGGGACAGT GATGAGCAGC CCATCTTCCT GACACCCTGC 
ATGCGGGACC TGATCAAGCT GGCTGGGGTC ACGCTGGGAC AGAGGCGAGC CCAGGCGAGG 
CGGCAGACCA TCAGGCATTC TACCAGGGAG AAGGACAGGG GACCCACGAA AGCCACCACC 
ACCAAGCTGG TCTACCAGAT CTTCGATACT TTCTTCGCAG AGCAAATTGA AAAGGATGAC 
AGAGAAGACA AGGAGAACGC CTTTAAGCGC CGGCGATGTG GCGTCTGTGA GGTGTGTCAG 
CAGCCTGAGT GTGGGAAATG TAAAGCCTGC AAGGACATGG TTAAATTTGG TGGCAGTGGA 
CGGAGCAAGC AGGCTTGCCA AGAGCGGAGG TGTCCCAATA TGGCCATGAA GGAGGCAGAT 
GACGATGAGG AAGTCGATGA TAACATCCCA GAGATGCCGT CACCCAAAAA AATGCACCAG 
GGGAAGAAGA AGAAACAGAA CAAGAATCGC ATCTCTTGGG TCGGAGAAGC CGTCAAGACT 
GATGGGAAGA AGAGTTACTA TAAGAAGGTG TGCATTGATG CGGAAACCCT GGAAGTGGGG 
GACTGTGTCT CTGTTATTCC AGATGATTCC TCAAAACCGC TGTATCTAGC AAGGGTCACG 
GCGCTGTGGG AGGACAGCAG CAACGGGCAG ATGTTTCACG CCCACTGGTT CTGCGCTGGG 
ACAGACACAG TCCTCGGGGC CACGTCGGAC CCTCTGGAGC TGTTCTTGGT GGATGAATGT 
GAGGACATGC AGCTTTCATA TATCCACAGC AAAGTGAAAG TCATCTACAA AGCCCCCTCC 
GAAAACTGGG CCATGGAGGG AGGCATGGAT CCCGAGTCCC TGCTGGAGGG GGACGACGGG 
AAGACCTACT TCTACCAGCT GTGGTATGAT CAAGACTACG CGAGATTCGA GTCCCCTCCA 
AAAACCCAGC CAACAGAGGA CAACAAGTTC AAATTCTGTG TGAGCTGTGC CCGTCTGGCT 
GAGATGAGGC AAAAAGAAAT CCCCAGGGTC CTGGAGCAGC TCGAGGACCT GGATAGCCGG 
GTCCTCTACT ACTCAGCCAC CAAGAACGGC ATCCTGTACC GAGTTGGTGA TGGTGTGTAC 
CTGCCCCCTG AGGCCTTCAC GTTCAACATC AAGCTGTCCA GTCCCGTGAA ACGCCCACGG 
AAGGAGCCCG TGGATGAGGA CCTGTACCCA GAGCACTACC GGAAATACTC CGACTACATC 
AAAGGCAGCA ACCTGGATGC CCCTGAGCCC TACCGAATTG GCCGGATCAA AGAGATCTTC 
TGTCCCAAGA AGAGCAACGG CAGGCCCAAT GAGACTGACA TCAAAATCCG GGTCAACAAG 
TTCTACAGGC CTGAGAACAC CCACAAGTCC ACTCCAGCGA GCTACCACGC AGACATCAAC 
CTGCTCTACT GGAGCGACGA GGAGGCCGTG GTGGACTTCA AGGCTGTGCA GGGCCGCTGC 
ACCGTGGAGT ATGGGGAGGA CCTGCCCGAG TGCGTCCAGG TGTACTCCAT GGGCGGCCCC 
AACCGCTTCT ACTTCCTCGA GGCCTATAAT GCAAAGAGCA AAAGCTTTGA AGATCCTCCC 
AACCATGCCC GTAGCCCTGG AAACAAAGGG AAGGGCAAGG GAAAAGGGAA GGGCAAGCCC 
AAGTCCCAAG CCTGTGAGCC GAGCGAGCCA GAGATAGAGA TCAAGCTGCC CAAGCTGCGG 
ACCCTGGATG TGTTTTCTGG CTGCGGGGGG TTGTCGGAGG GATTCCACCA AGCAGGCATC 
TCTGACACGC TGTGGGCCAT CGAGATGTGG GACCCTGCGG CCCAGGCGTT CCGGCTGAAC 
AACCCCGGCT CCACAGTGTT CACAGAGGAC TGCAACATCC TGCTGAAGCT GGTCATGGCT 
GGGGAGACCA CCAACTCCCG CGGCCAGCGG CTGCCCCAGA AGGGAGACGT GGAGATGCTG 
TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC GGCATGAACC GCTTCAATTC GCGCACCTAC 
TCCAAGTTCA AAAACTCTCT GGTGGTTTCC TTCCTCAGCT ACTGCGACTA CTACCGGCCC 
CGGTTCTTCC TCCTGGAGAA TGTCAGGAAC TTTGTCTCCT TCAAGCGCTC CATGGTCCTG 
AAGCTCACCC TCCGCTGCCT GGTCCGCATG GGCTATCAGT GCACCTTCGG CGTGCTGCAG 
GCCGGTCAGT ACGGCGTGGC CCAGACTAGG AGGCGGGCCA TCATCCTGGC CGCGGCCCCT 
GGAGAGAAGC TCCCTCTGTT CCCGGAGCCA CTGCACGTGT TTGCTCCCCG GGCCTGCCAG 
CTGAGCGTGG TGGTGGATGA CAAGAAGTTT GTGAGCAACA TAACCAGGTT GAGCTCGGGT 
CCTTTCCGGA CCATCACGGT GCGAGACACG ATGTCCGACC TGCCGGAGGT GCGGAATGGA 
GCCTCGGCAC TGGAGATCTC CTACAACGGG GAGCCTCAGT CCTGGTTCCA GAGGCAGCTC 
CGGGGCGCAC AGTACCAGCC CATCCTCAGG GACCACATCT GTAAGGACAT GAGTGCATTG 
GTGGCTGCCC GCATGCGGCA CATCCCCTTG GCCCCAGGGT CAGACTGGCG CGATCTGCCC 
AACATCGAGG TGCGGCTCTC AGACGGCACC ATGGCCAGGA AGCTGCGGTA TACCCACCAT 
GACAGGAAGA ACGGCCGCAG CAGCTCTGGG GCCCTCCGTG GGGTCTGCTC CTGCGTGGAA 
GGTGGGTCCT CCGGCAAAGC CTGCGACCCC GCAGCCAGGC AGTTCAACAC CCTCATCCCC 
TGGTGCCTGC CCCACACCGG GAACCGGCAC AACCACTGGG CTGGCCTCTA TGGAAGGCTC 
GAGTGGGACG GCTTCTTCAG CACAACCGTC ACCAACCCCG AGCCCATGGG CAAGCAGGGC 
CGCGTGCTCC ACCCAGAGCA GCACCGTGTG GTGAGCGTGC GGGAGTGTGC CCGCTCCCAG 
GGCTTCCCTG ACACCTACCG GCTCTTCGGC AACATCCTGG ACAAGCACCG GCAGGTGGGC 
AATGCCGTGC CACCGCCCCT GGCCAAAGCC ATTGGCTTGG AGATCAAGCT TTGTATGTTG 
GCCAAAGCCC GAGAGAGTGC CTCAGCTAAA ATAAAGGAGG AGGAAGCTGC TAAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNMT1 ( NM_001318730.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305659.1 Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 3, mRNA.

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CloneID	OHu57334
Clone ID Related Accession (Same CDS sequence)	NM_001318730.2 , NM_001318730.1
Accession Version	NM_001318730.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	DNA (cytosine-5)-methyltransferase 1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX379258.2, BC126227.1, X63692.1, BC092517.1, DA653750.1, AB209413.2 and HY245803.1. On Jan 9, 2016 this sequence version replaced XM_011527773.1. Transcript Variant: This variant (3) has a shorter 5' UTR, lacks an alternate in-frame exon in the 5' coding region, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209413.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCCTGAA CCTTCACCTA GCCCCAGGAT TACAAGGAAA 
AGCACCAGGC AAACCACCAT CACATCTCAT TTTGCAAAGG GCCCTGCCAA ACGGAAACCT 
CAGGAAGAGT CTGAAAGAGC CAAATCGGAT GAGTCCATCA AGGAAGAAGA CAAAGACCAG 
GATGAGAAGA GACGTAGAGT TACATCCAGA GAACGAGTTG CTAGACCGCT TCCTGCAGAA 
GAACCTGAAA GAGCAAAATC AGGAACGCGC ACTGAAAAGG AAGAAGAAAG AGATGAAAAA 
GAAGAAAAGA GACTCCGAAG TCAAACCAAA GAACCAACAC CCAAACAGAA ACTGAAGGAG 
GAGCCGGACA GAGAAGCCAG GGCAGGCGTG CAGGCTGACG AGGACGAAGA TGGAGACGAG 
AAAGATGAGA AGAAGCACAG AAGTCAACCC AAAGATCTAG CTGCCAAACG GAGGCCCGAA 
GAAAAAGAAC CTGAAAAAGT AAATCCACAG ATTTCTGATG AAAAAGACGA GGATGAAAAG 
GAGGAGAAGA GACGCAAAAC GACCCCCAAA GAACCAACGG AGAAAAAAAT GGCTCGCGCC 
AAAACAGTCA TGAACTCCAA GACCCACCCT CCCAAGTGCA TTCAGTGCGG GCAGTACCTG 
GACGACCCTG ACCTCAAATA TGGGCAGCAC CCACCAGACG CGGTGGATGA GCCACAGATG 
CTGACAAATG AGAAGCTGTC CATCTTTGAT GCCAACGAGT CTGGCTTTGA GAGTTATGAG 
GCGCTTCCCC AGCACAAACT GACCTGCTTC AGTGTGTACT GTAAGCACGG TCACCTGTGT 
CCCATCGACA CCGGCCTCAT CGAGAAGAAT ATCGAACTCT TCTTTTCTGG TTCAGCAAAA 
CCAATCTATG ATGATGACCC ATCTCTTGAA GGTGGTGTTA ATGGCAAAAA TCTTGGCCCC 
ATAAATGAAT GGTGGATCAC TGGCTTTGAT GGAGGTGAAA AGGCCCTCAT CGGCTTCAGC 
ACCTCATTTG CCGAATACAT TCTGATGGAT CCCAGTCCCG AGTATGCGCC CATATTTGGG 
CTGATGCAGG AGAAGATCTA CATCAGCAAG ATTGTGGTGG AGTTCCTGCA GAGCAATTCC 
GACTCGACCT ATGAGGACCT GATCAACAAG ATCGAGACCA CGGTTCCTCC TTCTGGCCTC 
AACTTGAACC GCTTCACAGA GGACTCCCTC CTGCGACACG CGCAGTTTGT GGTGGAGCAG 
GTGGAGAGTT ATGACGAGGC CGGGGACAGT GATGAGCAGC CCATCTTCCT GACACCCTGC 
ATGCGGGACC TGATCAAGCT GGCTGGGGTC ACGCTGGGAC AGAGGCGAGC CCAGGCGAGG 
CGGCAGACCA TCAGGCATTC TACCAGGGAG AAGGACAGGG GACCCACGAA AGCCACCACC 
ACCAAGCTGG TCTACCAGAT CTTCGATACT TTCTTCGCAG AGCAAATTGA AAAGGATGAC 
AGAGAAGACA AGGAGAACGC CTTTAAGCGC CGGCGATGTG GCGTCTGTGA GGTGTGTCAG 
CAGCCTGAGT GTGGGAAATG TAAAGCCTGC AAGGACATGG TTAAATTTGG TGGCAGTGGA 
CGGAGCAAGC AGGCTTGCCA AGAGCGGAGG TGTCCCAATA TGGCCATGAA GGAGGCAGAT 
GACGATGAGG AAGTCGATGA TAACATCCCA GAGATGCCGT CACCCAAAAA AATGCACCAG 
GGGAAGAAGA AGAAACAGAA CAAGAATCGC ATCTCTTGGG TCGGAGAAGC CGTCAAGACT 
GATGGGAAGA AGAGTTACTA TAAGAAGGTG TGCATTGATG CGGAAACCCT GGAAGTGGGG 
GACTGTGTCT CTGTTATTCC AGATGATTCC TCAAAACCGC TGTATCTAGC AAGGGTCACG 
GCGCTGTGGG AGGACAGCAG CAACGGGCAG ATGTTTCACG CCCACTGGTT CTGCGCTGGG 
ACAGACACAG TCCTCGGGGC CACGTCGGAC CCTCTGGAGC TGTTCTTGGT GGATGAATGT 
GAGGACATGC AGCTTTCATA TATCCACAGC AAAGTGAAAG TCATCTACAA AGCCCCCTCC 
GAAAACTGGG CCATGGAGGG AGGCATGGAT CCCGAGTCCC TGCTGGAGGG GGACGACGGG 
AAGACCTACT TCTACCAGCT GTGGTATGAT CAAGACTACG CGAGATTCGA GTCCCCTCCA 
AAAACCCAGC CAACAGAGGA CAACAAGTTC AAATTCTGTG TGAGCTGTGC CCGTCTGGCT 
GAGATGAGGC AAAAAGAAAT CCCCAGGGTC CTGGAGCAGC TCGAGGACCT GGATAGCCGG 
GTCCTCTACT ACTCAGCCAC CAAGAACGGC ATCCTGTACC GAGTTGGTGA TGGTGTGTAC 
CTGCCCCCTG AGGCCTTCAC GTTCAACATC AAGCTGTCCA GTCCCGTGAA ACGCCCACGG 
AAGGAGCCCG TGGATGAGGA CCTGTACCCA GAGCACTACC GGAAATACTC CGACTACATC 
AAAGGCAGCA ACCTGGATGC CCCTGAGCCC TACCGAATTG GCCGGATCAA AGAGATCTTC 
TGTCCCAAGA AGAGCAACGG CAGGCCCAAT GAGACTGACA TCAAAATCCG GGTCAACAAG 
TTCTACAGGC CTGAGAACAC CCACAAGTCC ACTCCAGCGA GCTACCACGC AGACATCAAC 
CTGCTCTACT GGAGCGACGA GGAGGCCGTG GTGGACTTCA AGGCTGTGCA GGGCCGCTGC 
ACCGTGGAGT ATGGGGAGGA CCTGCCCGAG TGCGTCCAGG TGTACTCCAT GGGCGGCCCC 
AACCGCTTCT ACTTCCTCGA GGCCTATAAT GCAAAGAGCA AAAGCTTTGA AGATCCTCCC 
AACCATGCCC GTAGCCCTGG AAACAAAGGG AAGGGCAAGG GAAAAGGGAA GGGCAAGCCC 
AAGTCCCAAG CCTGTGAGCC GAGCGAGCCA GAGATAGAGA TCAAGCTGCC CAAGCTGCGG 
ACCCTGGATG TGTTTTCTGG CTGCGGGGGG TTGTCGGAGG GATTCCACCA AGCAGGCATC 
TCTGACACGC TGTGGGCCAT CGAGATGTGG GACCCTGCGG CCCAGGCGTT CCGGCTGAAC 
AACCCCGGCT CCACAGTGTT CACAGAGGAC TGCAACATCC TGCTGAAGCT GGTCATGGCT 
GGGGAGACCA CCAACTCCCG CGGCCAGCGG CTGCCCCAGA AGGGAGACGT GGAGATGCTG 
TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC GGCATGAACC GCTTCAATTC GCGCACCTAC 
TCCAAGTTCA AAAACTCTCT GGTGGTTTCC TTCCTCAGCT ACTGCGACTA CTACCGGCCC 
CGGTTCTTCC TCCTGGAGAA TGTCAGGAAC TTTGTCTCCT TCAAGCGCTC CATGGTCCTG 
AAGCTCACCC TCCGCTGCCT GGTCCGCATG GGCTATCAGT GCACCTTCGG CGTGCTGCAG 
GCCGGTCAGT ACGGCGTGGC CCAGACTAGG AGGCGGGCCA TCATCCTGGC CGCGGCCCCT 
GGAGAGAAGC TCCCTCTGTT CCCGGAGCCA CTGCACGTGT TTGCTCCCCG GGCCTGCCAG 
CTGAGCGTGG TGGTGGATGA CAAGAAGTTT GTGAGCAACA TAACCAGGTT GAGCTCGGGT 
CCTTTCCGGA CCATCACGGT GCGAGACACG ATGTCCGACC TGCCGGAGGT GCGGAATGGA 
GCCTCGGCAC TGGAGATCTC CTACAACGGG GAGCCTCAGT CCTGGTTCCA GAGGCAGCTC 
CGGGGCGCAC AGTACCAGCC CATCCTCAGG GACCACATCT GTAAGGACAT GAGTGCATTG 
GTGGCTGCCC GCATGCGGCA CATCCCCTTG GCCCCAGGGT CAGACTGGCG CGATCTGCCC 
AACATCGAGG TGCGGCTCTC AGACGGCACC ATGGCCAGGA AGCTGCGGTA TACCCACCAT 
GACAGGAAGA ACGGCCGCAG CAGCTCTGGG GCCCTCCGTG GGGTCTGCTC CTGCGTGGAA 
GGTGGGTCCT CCGGCAAAGC CTGCGACCCC GCAGCCAGGC AGTTCAACAC CCTCATCCCC 
TGGTGCCTGC CCCACACCGG GAACCGGCAC AACCACTGGG CTGGCCTCTA TGGAAGGCTC 
GAGTGGGACG GCTTCTTCAG CACAACCGTC ACCAACCCCG AGCCCATGGG CAAGCAGGGC 
CGCGTGCTCC ACCCAGAGCA GCACCGTGTG GTGAGCGTGC GGGAGTGTGC CCGCTCCCAG 
GGCTTCCCTG ACACCTACCG GCTCTTCGGC AACATCCTGG ACAAGCACCG GCAGGTGGGC 
AATGCCGTGC CACCGCCCCT GGCCAAAGCC ATTGGCTTGG AGATCAAGCT TTGTATGTTG 
GCCAAAGCCC GAGAGAGTGC CTCAGCTAAA ATAAAGGAGG AGGAAGCTGC TAAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305659.1
CDS	156..5015
Translation	MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEGGSSGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

Target ORF information:

RefSeq Version	NM_001318730.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA methyltransferase 1 (DNMT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318730.1

ORF Insert Sequence:

ATGCCGGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 
CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 
TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 
TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 
AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 
CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 
GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 
AGCAAGTCCG ATGGAGAGGC TAAGCCTGAA CCTTCACCTA GCCCCAGGAT TACAAGGAAA 
AGCACCAGGC AAACCACCAT CACATCTCAT TTTGCAAAGG GCCCTGCCAA ACGGAAACCT 
CAGGAAGAGT CTGAAAGAGC CAAATCGGAT GAGTCCATCA AGGAAGAAGA CAAAGACCAG 
GATGAGAAGA GACGTAGAGT TACATCCAGA GAACGAGTTG CTAGACCGCT TCCTGCAGAA 
GAACCTGAAA GAGCAAAATC AGGAACGCGC ACTGAAAAGG AAGAAGAAAG AGATGAAAAA 
GAAGAAAAGA GACTCCGAAG TCAAACCAAA GAACCAACAC CCAAACAGAA ACTGAAGGAG 
GAGCCGGACA GAGAAGCCAG GGCAGGCGTG CAGGCTGACG AGGACGAAGA TGGAGACGAG 
AAAGATGAGA AGAAGCACAG AAGTCAACCC AAAGATCTAG CTGCCAAACG GAGGCCCGAA 
GAAAAAGAAC CTGAAAAAGT AAATCCACAG ATTTCTGATG AAAAAGACGA GGATGAAAAG 
GAGGAGAAGA GACGCAAAAC GACCCCCAAA GAACCAACGG AGAAAAAAAT GGCTCGCGCC 
AAAACAGTCA TGAACTCCAA GACCCACCCT CCCAAGTGCA TTCAGTGCGG GCAGTACCTG 
GACGACCCTG ACCTCAAATA TGGGCAGCAC CCACCAGACG CGGTGGATGA GCCACAGATG 
CTGACAAATG AGAAGCTGTC CATCTTTGAT GCCAACGAGT CTGGCTTTGA GAGTTATGAG 
GCGCTTCCCC AGCACAAACT GACCTGCTTC AGTGTGTACT GTAAGCACGG TCACCTGTGT 
CCCATCGACA CCGGCCTCAT CGAGAAGAAT ATCGAACTCT TCTTTTCTGG TTCAGCAAAA 
CCAATCTATG ATGATGACCC ATCTCTTGAA GGTGGTGTTA ATGGCAAAAA TCTTGGCCCC 
ATAAATGAAT GGTGGATCAC TGGCTTTGAT GGAGGTGAAA AGGCCCTCAT CGGCTTCAGC 
ACCTCATTTG CCGAATACAT TCTGATGGAT CCCAGTCCCG AGTATGCGCC CATATTTGGG 
CTGATGCAGG AGAAGATCTA CATCAGCAAG ATTGTGGTGG AGTTCCTGCA GAGCAATTCC 
GACTCGACCT ATGAGGACCT GATCAACAAG ATCGAGACCA CGGTTCCTCC TTCTGGCCTC 
AACTTGAACC GCTTCACAGA GGACTCCCTC CTGCGACACG CGCAGTTTGT GGTGGAGCAG 
GTGGAGAGTT ATGACGAGGC CGGGGACAGT GATGAGCAGC CCATCTTCCT GACACCCTGC 
ATGCGGGACC TGATCAAGCT GGCTGGGGTC ACGCTGGGAC AGAGGCGAGC CCAGGCGAGG 
CGGCAGACCA TCAGGCATTC TACCAGGGAG AAGGACAGGG GACCCACGAA AGCCACCACC 
ACCAAGCTGG TCTACCAGAT CTTCGATACT TTCTTCGCAG AGCAAATTGA AAAGGATGAC 
AGAGAAGACA AGGAGAACGC CTTTAAGCGC CGGCGATGTG GCGTCTGTGA GGTGTGTCAG 
CAGCCTGAGT GTGGGAAATG TAAAGCCTGC AAGGACATGG TTAAATTTGG TGGCAGTGGA 
CGGAGCAAGC AGGCTTGCCA AGAGCGGAGG TGTCCCAATA TGGCCATGAA GGAGGCAGAT 
GACGATGAGG AAGTCGATGA TAACATCCCA GAGATGCCGT CACCCAAAAA AATGCACCAG 
GGGAAGAAGA AGAAACAGAA CAAGAATCGC ATCTCTTGGG TCGGAGAAGC CGTCAAGACT 
GATGGGAAGA AGAGTTACTA TAAGAAGGTG TGCATTGATG CGGAAACCCT GGAAGTGGGG 
GACTGTGTCT CTGTTATTCC AGATGATTCC TCAAAACCGC TGTATCTAGC AAGGGTCACG 
GCGCTGTGGG AGGACAGCAG CAACGGGCAG ATGTTTCACG CCCACTGGTT CTGCGCTGGG 
ACAGACACAG TCCTCGGGGC CACGTCGGAC CCTCTGGAGC TGTTCTTGGT GGATGAATGT 
GAGGACATGC AGCTTTCATA TATCCACAGC AAAGTGAAAG TCATCTACAA AGCCCCCTCC 
GAAAACTGGG CCATGGAGGG AGGCATGGAT CCCGAGTCCC TGCTGGAGGG GGACGACGGG 
AAGACCTACT TCTACCAGCT GTGGTATGAT CAAGACTACG CGAGATTCGA GTCCCCTCCA 
AAAACCCAGC CAACAGAGGA CAACAAGTTC AAATTCTGTG TGAGCTGTGC CCGTCTGGCT 
GAGATGAGGC AAAAAGAAAT CCCCAGGGTC CTGGAGCAGC TCGAGGACCT GGATAGCCGG 
GTCCTCTACT ACTCAGCCAC CAAGAACGGC ATCCTGTACC GAGTTGGTGA TGGTGTGTAC 
CTGCCCCCTG AGGCCTTCAC GTTCAACATC AAGCTGTCCA GTCCCGTGAA ACGCCCACGG 
AAGGAGCCCG TGGATGAGGA CCTGTACCCA GAGCACTACC GGAAATACTC CGACTACATC 
AAAGGCAGCA ACCTGGATGC CCCTGAGCCC TACCGAATTG GCCGGATCAA AGAGATCTTC 
TGTCCCAAGA AGAGCAACGG CAGGCCCAAT GAGACTGACA TCAAAATCCG GGTCAACAAG 
TTCTACAGGC CTGAGAACAC CCACAAGTCC ACTCCAGCGA GCTACCACGC AGACATCAAC 
CTGCTCTACT GGAGCGACGA GGAGGCCGTG GTGGACTTCA AGGCTGTGCA GGGCCGCTGC 
ACCGTGGAGT ATGGGGAGGA CCTGCCCGAG TGCGTCCAGG TGTACTCCAT GGGCGGCCCC 
AACCGCTTCT ACTTCCTCGA GGCCTATAAT GCAAAGAGCA AAAGCTTTGA AGATCCTCCC 
AACCATGCCC GTAGCCCTGG AAACAAAGGG AAGGGCAAGG GAAAAGGGAA GGGCAAGCCC 
AAGTCCCAAG CCTGTGAGCC GAGCGAGCCA GAGATAGAGA TCAAGCTGCC CAAGCTGCGG 
ACCCTGGATG TGTTTTCTGG CTGCGGGGGG TTGTCGGAGG GATTCCACCA AGCAGGCATC 
TCTGACACGC TGTGGGCCAT CGAGATGTGG GACCCTGCGG CCCAGGCGTT CCGGCTGAAC 
AACCCCGGCT CCACAGTGTT CACAGAGGAC TGCAACATCC TGCTGAAGCT GGTCATGGCT 
GGGGAGACCA CCAACTCCCG CGGCCAGCGG CTGCCCCAGA AGGGAGACGT GGAGATGCTG 
TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC GGCATGAACC GCTTCAATTC GCGCACCTAC 
TCCAAGTTCA AAAACTCTCT GGTGGTTTCC TTCCTCAGCT ACTGCGACTA CTACCGGCCC 
CGGTTCTTCC TCCTGGAGAA TGTCAGGAAC TTTGTCTCCT TCAAGCGCTC CATGGTCCTG 
AAGCTCACCC TCCGCTGCCT GGTCCGCATG GGCTATCAGT GCACCTTCGG CGTGCTGCAG 
GCCGGTCAGT ACGGCGTGGC CCAGACTAGG AGGCGGGCCA TCATCCTGGC CGCGGCCCCT 
GGAGAGAAGC TCCCTCTGTT CCCGGAGCCA CTGCACGTGT TTGCTCCCCG GGCCTGCCAG 
CTGAGCGTGG TGGTGGATGA CAAGAAGTTT GTGAGCAACA TAACCAGGTT GAGCTCGGGT 
CCTTTCCGGA CCATCACGGT GCGAGACACG ATGTCCGACC TGCCGGAGGT GCGGAATGGA 
GCCTCGGCAC TGGAGATCTC CTACAACGGG GAGCCTCAGT CCTGGTTCCA GAGGCAGCTC 
CGGGGCGCAC AGTACCAGCC CATCCTCAGG GACCACATCT GTAAGGACAT GAGTGCATTG 
GTGGCTGCCC GCATGCGGCA CATCCCCTTG GCCCCAGGGT CAGACTGGCG CGATCTGCCC 
AACATCGAGG TGCGGCTCTC AGACGGCACC ATGGCCAGGA AGCTGCGGTA TACCCACCAT 
GACAGGAAGA ACGGCCGCAG CAGCTCTGGG GCCCTCCGTG GGGTCTGCTC CTGCGTGGAA 
GGTGGGTCCT CCGGCAAAGC CTGCGACCCC GCAGCCAGGC AGTTCAACAC CCTCATCCCC 
TGGTGCCTGC CCCACACCGG GAACCGGCAC AACCACTGGG CTGGCCTCTA TGGAAGGCTC 
GAGTGGGACG GCTTCTTCAG CACAACCGTC ACCAACCCCG AGCCCATGGG CAAGCAGGGC 
CGCGTGCTCC ACCCAGAGCA GCACCGTGTG GTGAGCGTGC GGGAGTGTGC CCGCTCCCAG 
GGCTTCCCTG ACACCTACCG GCTCTTCGGC AACATCCTGG ACAAGCACCG GCAGGTGGGC 
AATGCCGTGC CACCGCCCCT GGCCAAAGCC ATTGGCTTGG AGATCAAGCT TTGTATGTTG 
GCCAAAGCCC GAGAGAGTGC CTCAGCTAAA ATAAAGGAGG AGGAAGCTGC TAAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DNMT1
Entrez Gene ID	1786
Full Name	DNA methyltransferase 1
Synonyms	ADCADN,AIM,CXXC9,DNMT,HSN1E,MCMT,m.HsaI
General protein information	Preferred Names DNA methyltransferase 1 Names DNA (cytosine-5)-methyltransferase 1 CXXC-type zinc finger protein 9 DNA (cytosine-5-)-methyltransferase 1 DNA MTase HsaI DNA methyltransferase HsaI
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.2
Summary	This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016].
Disorder MIM:	126375
Disorder Html:	Neuropathy, hereditary sensory, type IE, 614116 (3); Cerebellar ataxia, deafness, and narcolepsy, autosomal dominant, 604121 (3)

Arabidopsis thaliana (thale cress)	MEE57	NP_193097.1
Homo sapiens (human)	DNMT1	NP_001124295.1
Bos taurus (cattle)	DNMT1	NP_872592.2
Danio rerio (zebrafish)	dnmt1	NP_571264.1
Oryza sativa (rice)	Os03g0798300	NP_001051569.1
Oryza sativa (rice)	Os07g0182900	NP_001059052.1
Canis lupus familiaris (dog)	DNMT1	XP_533919.4
Arabidopsis thaliana (thale cress)	AT4G08990	NP_192638.1
Xenopus tropicalis (tropical clawed frog)	dnmt1	XP_002934384.1
Pan troglodytes (chimpanzee)	DNMT1	XP_512361.3
	DNMT1	XP_001104704.2
Mus musculus (house mouse)	Dnmt1	NP_001186360.2
Rattus norvegicus (Norway rat)	Dnmt1	NP_445806.3
Arabidopsis thaliana (thale cress)	DMT2	NP_193150.1
Arabidopsis thaliana (thale cress)	MET1	NP_199727.1
Gallus gallus (chicken)	DNMT1	NP_996835.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DNMT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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