DNMT1 cDNA ORF clone, Bos taurus(cattle)

The following DNMT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNMT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa235760 XM_024993829.1
Latest version!
Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa235761 XM_024993830.1
Latest version!
Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa235761 XM_024993831.1
Latest version!
Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa235762 XM_024993832.1
Latest version!
Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa91052 XM_015471991.2
Latest version!
Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa91053 XM_015471992.2
Latest version!
Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa91054 XM_015471993.1
Latest version!
Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa91055 XM_015471995.2
Latest version!
Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa91056 XM_015471996.1
Latest version!
Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa91057 XM_015471997.1
Latest version!
Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa44289 NM_182651.2
Latest version!
Bos taurus DNA methyltransferase 1 (DNMT1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45072 XM_005208712.4
Latest version!
Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45073 XM_010806776.3
Latest version!
Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OBa45073 XM_010806776.2 Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa91052 XM_015471991.1 Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa45072 XM_005208712.3 Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa91055 XM_015471995.1 Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa91053 XM_015471992.1 Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa235760
    Clone ID Related Accession (Same CDS sequence) XM_024993829.1
    Accession Version XM_024993829.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGCTGGACT TTCTACTTGT
    ATATTTGCCT ATGTCATCAA CTGCCCAGAT CAAGATGCAG AAAATTCCCT TCACCTGCCT
    GGCACTGCAA GCTGGTTCCA TCTCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849597.1
    CDS79..5028
    Translation

    Target ORF information:

    RefSeq Version XM_024993829.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993829.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGCTGGACT TTCTACTTGT
    ATATTTGCCT ATGTCATCAA CTGCCCAGAT CAAGATGCAG AAAATTCCCT TCACCTGCCT
    GGCACTGCAA GCTGGTTCCA TCTCCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235761
    Clone ID Related Accession (Same CDS sequence) XM_024993831.1 , XM_024993830.1
    Accession Version XM_024993831.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGGAGGAG
    AAGAGACGCA GAACTACATA CAGAGAACTA ACCGAGAAGA AAATGACTCG AACCAAAATA
    GCCGTAGTGT CCAAGACCAA TCCTCCGAAG TGCACCGAGT GCTTGCAGTA CCTGGACGAC
    CCTGAGCTGA GATACGAGCA GCACCCCCCC GATGCGGTGG AAGAGATACA GATACTGACC
    AACGAGAGGT TGTCCATCTT TGATGCCAAC GAATCTGGCT TTGAGAGTTA CGAGGATTTG
    CCTCAGCACA AACTAACCTG CTTCAGCGTG TACTGTAAAC GCGGTCACCT TTGCCCGATC
    GACACCGGCC TCATTGAGAA GGATGTCGAG CTCCTCTTTT CTGGTTCAGC AAAGCCGATA
    TATGAGGATG ACCCATCTCC CGAAGGTGGT ATTAATGGCA AAAATTTTGG CCCCATAAAC
    GAATGGTGGA TTGCTGGTTT TGATGGAGGT GAAAAGGCTC TTCTTGGCTT TAGCACCTCA
    TTTGCCGAGT ATATCTTGAT GGATCCCAGC CCAGAGTACG CACCACTATT CAGCGTGATG
    CAGGAGAAGA TCTATATAAG TAAGATAGTG GTTGAGTTCC TGCAGAGCAA CCCTGACTCC
    ACCTACGAAG ACCTGATCAA TAAGATTGAG ACCACCGTTC CTCCTTGTAT GCTCAACTTG
    AATCGATTCA CAGAGGATTC TCTCCTGCGG CATGCCCAGT TCGTGGTGGA GCAAGTAGAG
    AGTTATGATC GGGCTGGGGA CAGTGACGAG CAGCCCATCT TCCTGAGCCC CTGCATGAGA
    GACCTCATCA AGCTGGCCGG GGTCACCCTG GGAAAAAGGC GAGCCGAGAG GCGGCAGACC
    ATCCGGCAAC CCGCCAAAGA GAAGGACAAG GGCCCCACCA AGGCCACCAC CACCAAGCTG
    GTCTACCAGA TCTTTGACAC TTTCTTTGCG GAGCAAATTG AAAAAGATGA CAAGGAAGAC
    AAGGAGAATG CCTTCAAGCG CCGGCGCTGT GGCGTCTGTG AGATTTGTCA ACAGCCCGAG
    TGTGGAAAGT GTAAGGCCTG TAAGGATATG GTTAAATTTG GTGGTAGCGG ACGGAGCAAG
    CAGGCTTGCC AAAAGAGGAG GTGTCCCAAC ATGGCCATGA AGGAGGCAGA CGATGACGAG
    GAAGTGGATG ACAATATTCC AGAGATGCCA TCACCCAAAA AGATGCATCA GGGGAAGAAA
    AAGAAGCAGA ATAAGAATCG GATCTCTTGG GTTGGCGATG CCGTCAAGAC TGACGGGAAG
    AAGAGTTACT ACAAGAAGGT ATGCATCGAC TCGGAAACCC TGGAAGTGGG GGACTGTGTT
    TCTGTAATTC CAGACGACTC TTCAAAACCA CTGTATCTAG CAAGGGTCAC GGCGCTGTGG
    GAGGACAGCA GCAATGGGCA GATGTTCCAT GCCCACTGGT TCTGTGCTGG GACGGACACG
    GTCCTCGGGG CCACATCGGA CCCCCTGGAG CTGTTCCTGG TTGACGAGTG TGAGGACATG
    CAGCTCTCGT ACATCCACAG CAAGGTGCAG GTCATTTATA AGGCGCCCTC AGAGAACTGG
    GCCATGGAGG GAGGCGTGGA CCCCGAGGCC CTGATGTCAG AGGACGACGG GAAGACCTAC
    TTCTACCAGC TGTGGTACGA CCAAGACTAC GCGAGATTTG AGTCCCCTCC GAAAACTCAG
    CCGACGGAGG ACAACAAGTA CAAGTTCTGC GCAAGCTGTG CACGTCTGGC CGAAATGAGG
    CAGAAGGAAA TCCCCAGGGT CGTGGAGCAG CTCCAGGACC TGGAAGGCCG CGTCCTCTAC
    AGCCTCGCCA CCAAGAACGG CGTCCAGTAC CGGGTGGGCG ATGGCGTGTA CCTCCCTCCC
    GAGGCCTTCA CCTTCAACAT CAAGCTGTCC AGTCCTGTGA AACGCCCCCG GAAGGAGCCT
    GTGGACGAAG CTCTGTATCC AGAACACTAC CGGAAGTACT CTGACTACAT CAAGGGCAGC
    AACCTGGATG CCCCTGAGCC CTACCGTATT GGCCGCATAA AGGAGATCTT CTGCAGCAAG
    AAGAGCAACG GCCGGCCCAA TGAGACAGAC ATCAAGATCA GGGTCAACAA GTTCTACAGG
    CCGGAGAACA CACACAAGTC TACCCCAGCC AGTTACCACG CAGACATCAA CCTGCTTTAC
    TGGAGCGATG AGGAGGCCGT GGTGGACTTC AAGGCCGTGC AGGGCCGCTG CACCGTGGAG
    TACGGAGAGG ACCTGCCTCA GTGCCTCCAG GACTTCTCCG CTGGTGGCCC CGATCGCTTC
    TATTTTCTCG AGGCCTATAA CGCCAAGAGC AAAAGCTTTG AAGATCCTCC GAACCACGCC
    CGGAGCACCG GAAATAAAGG GAAAGGGAAG GGGAAAGGAA AAAACAGGAC GAAATCTCAG
    ACGTGTGAGC CGAGTGAACT GGAGACAGAA ATCAAACTGC CGAAGCTGCG GACCCTGGAC
    GTGTTTTCCG GCTGTGGGGG ATTGTCGGAA GGCTTCCACC AAGCAGGCAT CTCGGAGACA
    CTTTGGGCCA TCGAGATGTG GGACCCTGCG GCCCAGGCGT TCCGGCTCAA CAACCCTGGG
    TCCACGGTGT TCACAGAGGA CTGCAACGTC CTGCTGAAGC TGGTCATGGC TGGGGAGGTG
    ACCAACTCCC GCGGCCAGAA GCTGCCTCAG AAGGGAGATG TGGAGATGCT GTGCGGCGGG
    CCGCCCTGCC AGGGCTTCAG CGGCATGAAC CGCTTCAACT CTCGAACCTA CTCCAAATTC
    AAGAACTCTC TGGTGGTCTC TTTCCTCAGC TACTGTGACT ACTACCGGCC CCGCTACTTC
    CTCTTGGAGA ACGTTCGGAA CTTCGTCTCC TTCAAGCGCT CCATGGTCCT GAAGCTGACG
    CTGCGCTGCC TGGTCCGCAT GGGCTACCAG TGCACCTTTG GCGTGCTGCA GGCTGGTCAG
    TACGGCGTGG CCCAGACTCG GAGGCGAGCC ATCATCCTGG CTGCAGCCCC TGGGGAGCCA
    CTCCCGCTGT TCCCGGAGCC GTTGCATGTG TTCGCACCCC GGGCCTGCCA GCTGAGCGTC
    GTAGTGGACG ACAAGAAGTT TGTCAGCAAC ATCACCAGGT TGAGCTCGGG TCCCTTCCGA
    ACCATCACCG TGCGGGACAC CATGTCTGAC CTCCCTGAGA TCCGGAACGG GGCCTCGGCA
    CTGGAGATTT CATACAACGG GGAGCCCCAG TCCTGGTTCC AGAGGCAGCT CCGGGGCTCG
    CAGTACCAGC CCATCCTCAG GGATCATATT TGCAAGGACA TGAGCGCCTT GGTGGCTGCC
    CGCATGCGGC ACATCCCCCT GGCCCCGGGC TCGGACTGGC GTGACCTGCC CAACATTGAG
    GTGCGGCTCT CTGACGGCAC CCTGGCCCGG AAGCTGCGGT ACAACTACCA CGACAAGAAG
    AACGGCTGCA GCAGCAGCGG CGCCCTCCGT GGGGTCTGCT CCTGTGTGGA AGGCAAGCCC
    TGTGAGCCTG CGGCCCGACA GTTTAACACC CTTATCCCCT GGTGCCTGCC CCACACTGGG
    AACAGGCACA ACCACTGGGC CGGCCTCTAC GGGCGTCTCG AGTGGGACGG CTTCTTCAGC
    ACAACTGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCCGAGCAG
    CACCGAGTGG TGAGCGTCCG GGAGTGCGCC CGCTCCCAGG GCTTCCCCGA CACCTATCGG
    CTGTTCGGCA ACATCCTAGA CAAGCACCGG CAGGTGGGTA ATGCTGTGCC GCCGCCACTG
    GCCAAAGCCA TCGGCTTGGA GATCAAGCGC TGCATGTTGG CCAAAGCGCG CGAGAGCGCC
    TCAGCTGGAC TTTCTACTTG TATATTTGCC TATGTCATCA ACTGCCCAGA TCAAGATGCA
    GAAAATTCCC TTCACCTGCC TGGCACTGCA AGCTGGTTCC ATCTCCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849599.1
    CDS79..4989
    Translation

    Target ORF information:

    RefSeq Version XM_024993831.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993831.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGGAGGAG
    AAGAGACGCA GAACTACATA CAGAGAACTA ACCGAGAAGA AAATGACTCG AACCAAAATA
    GCCGTAGTGT CCAAGACCAA TCCTCCGAAG TGCACCGAGT GCTTGCAGTA CCTGGACGAC
    CCTGAGCTGA GATACGAGCA GCACCCCCCC GATGCGGTGG AAGAGATACA GATACTGACC
    AACGAGAGGT TGTCCATCTT TGATGCCAAC GAATCTGGCT TTGAGAGTTA CGAGGATTTG
    CCTCAGCACA AACTAACCTG CTTCAGCGTG TACTGTAAAC GCGGTCACCT TTGCCCGATC
    GACACCGGCC TCATTGAGAA GGATGTCGAG CTCCTCTTTT CTGGTTCAGC AAAGCCGATA
    TATGAGGATG ACCCATCTCC CGAAGGTGGT ATTAATGGCA AAAATTTTGG CCCCATAAAC
    GAATGGTGGA TTGCTGGTTT TGATGGAGGT GAAAAGGCTC TTCTTGGCTT TAGCACCTCA
    TTTGCCGAGT ATATCTTGAT GGATCCCAGC CCAGAGTACG CACCACTATT CAGCGTGATG
    CAGGAGAAGA TCTATATAAG TAAGATAGTG GTTGAGTTCC TGCAGAGCAA CCCTGACTCC
    ACCTACGAAG ACCTGATCAA TAAGATTGAG ACCACCGTTC CTCCTTGTAT GCTCAACTTG
    AATCGATTCA CAGAGGATTC TCTCCTGCGG CATGCCCAGT TCGTGGTGGA GCAAGTAGAG
    AGTTATGATC GGGCTGGGGA CAGTGACGAG CAGCCCATCT TCCTGAGCCC CTGCATGAGA
    GACCTCATCA AGCTGGCCGG GGTCACCCTG GGAAAAAGGC GAGCCGAGAG GCGGCAGACC
    ATCCGGCAAC CCGCCAAAGA GAAGGACAAG GGCCCCACCA AGGCCACCAC CACCAAGCTG
    GTCTACCAGA TCTTTGACAC TTTCTTTGCG GAGCAAATTG AAAAAGATGA CAAGGAAGAC
    AAGGAGAATG CCTTCAAGCG CCGGCGCTGT GGCGTCTGTG AGATTTGTCA ACAGCCCGAG
    TGTGGAAAGT GTAAGGCCTG TAAGGATATG GTTAAATTTG GTGGTAGCGG ACGGAGCAAG
    CAGGCTTGCC AAAAGAGGAG GTGTCCCAAC ATGGCCATGA AGGAGGCAGA CGATGACGAG
    GAAGTGGATG ACAATATTCC AGAGATGCCA TCACCCAAAA AGATGCATCA GGGGAAGAAA
    AAGAAGCAGA ATAAGAATCG GATCTCTTGG GTTGGCGATG CCGTCAAGAC TGACGGGAAG
    AAGAGTTACT ACAAGAAGGT ATGCATCGAC TCGGAAACCC TGGAAGTGGG GGACTGTGTT
    TCTGTAATTC CAGACGACTC TTCAAAACCA CTGTATCTAG CAAGGGTCAC GGCGCTGTGG
    GAGGACAGCA GCAATGGGCA GATGTTCCAT GCCCACTGGT TCTGTGCTGG GACGGACACG
    GTCCTCGGGG CCACATCGGA CCCCCTGGAG CTGTTCCTGG TTGACGAGTG TGAGGACATG
    CAGCTCTCGT ACATCCACAG CAAGGTGCAG GTCATTTATA AGGCGCCCTC AGAGAACTGG
    GCCATGGAGG GAGGCGTGGA CCCCGAGGCC CTGATGTCAG AGGACGACGG GAAGACCTAC
    TTCTACCAGC TGTGGTACGA CCAAGACTAC GCGAGATTTG AGTCCCCTCC GAAAACTCAG
    CCGACGGAGG ACAACAAGTA CAAGTTCTGC GCAAGCTGTG CACGTCTGGC CGAAATGAGG
    CAGAAGGAAA TCCCCAGGGT CGTGGAGCAG CTCCAGGACC TGGAAGGCCG CGTCCTCTAC
    AGCCTCGCCA CCAAGAACGG CGTCCAGTAC CGGGTGGGCG ATGGCGTGTA CCTCCCTCCC
    GAGGCCTTCA CCTTCAACAT CAAGCTGTCC AGTCCTGTGA AACGCCCCCG GAAGGAGCCT
    GTGGACGAAG CTCTGTATCC AGAACACTAC CGGAAGTACT CTGACTACAT CAAGGGCAGC
    AACCTGGATG CCCCTGAGCC CTACCGTATT GGCCGCATAA AGGAGATCTT CTGCAGCAAG
    AAGAGCAACG GCCGGCCCAA TGAGACAGAC ATCAAGATCA GGGTCAACAA GTTCTACAGG
    CCGGAGAACA CACACAAGTC TACCCCAGCC AGTTACCACG CAGACATCAA CCTGCTTTAC
    TGGAGCGATG AGGAGGCCGT GGTGGACTTC AAGGCCGTGC AGGGCCGCTG CACCGTGGAG
    TACGGAGAGG ACCTGCCTCA GTGCCTCCAG GACTTCTCCG CTGGTGGCCC CGATCGCTTC
    TATTTTCTCG AGGCCTATAA CGCCAAGAGC AAAAGCTTTG AAGATCCTCC GAACCACGCC
    CGGAGCACCG GAAATAAAGG GAAAGGGAAG GGGAAAGGAA AAAACAGGAC GAAATCTCAG
    ACGTGTGAGC CGAGTGAACT GGAGACAGAA ATCAAACTGC CGAAGCTGCG GACCCTGGAC
    GTGTTTTCCG GCTGTGGGGG ATTGTCGGAA GGCTTCCACC AAGCAGGCAT CTCGGAGACA
    CTTTGGGCCA TCGAGATGTG GGACCCTGCG GCCCAGGCGT TCCGGCTCAA CAACCCTGGG
    TCCACGGTGT TCACAGAGGA CTGCAACGTC CTGCTGAAGC TGGTCATGGC TGGGGAGGTG
    ACCAACTCCC GCGGCCAGAA GCTGCCTCAG AAGGGAGATG TGGAGATGCT GTGCGGCGGG
    CCGCCCTGCC AGGGCTTCAG CGGCATGAAC CGCTTCAACT CTCGAACCTA CTCCAAATTC
    AAGAACTCTC TGGTGGTCTC TTTCCTCAGC TACTGTGACT ACTACCGGCC CCGCTACTTC
    CTCTTGGAGA ACGTTCGGAA CTTCGTCTCC TTCAAGCGCT CCATGGTCCT GAAGCTGACG
    CTGCGCTGCC TGGTCCGCAT GGGCTACCAG TGCACCTTTG GCGTGCTGCA GGCTGGTCAG
    TACGGCGTGG CCCAGACTCG GAGGCGAGCC ATCATCCTGG CTGCAGCCCC TGGGGAGCCA
    CTCCCGCTGT TCCCGGAGCC GTTGCATGTG TTCGCACCCC GGGCCTGCCA GCTGAGCGTC
    GTAGTGGACG ACAAGAAGTT TGTCAGCAAC ATCACCAGGT TGAGCTCGGG TCCCTTCCGA
    ACCATCACCG TGCGGGACAC CATGTCTGAC CTCCCTGAGA TCCGGAACGG GGCCTCGGCA
    CTGGAGATTT CATACAACGG GGAGCCCCAG TCCTGGTTCC AGAGGCAGCT CCGGGGCTCG
    CAGTACCAGC CCATCCTCAG GGATCATATT TGCAAGGACA TGAGCGCCTT GGTGGCTGCC
    CGCATGCGGC ACATCCCCCT GGCCCCGGGC TCGGACTGGC GTGACCTGCC CAACATTGAG
    GTGCGGCTCT CTGACGGCAC CCTGGCCCGG AAGCTGCGGT ACAACTACCA CGACAAGAAG
    AACGGCTGCA GCAGCAGCGG CGCCCTCCGT GGGGTCTGCT CCTGTGTGGA AGGCAAGCCC
    TGTGAGCCTG CGGCCCGACA GTTTAACACC CTTATCCCCT GGTGCCTGCC CCACACTGGG
    AACAGGCACA ACCACTGGGC CGGCCTCTAC GGGCGTCTCG AGTGGGACGG CTTCTTCAGC
    ACAACTGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCCGAGCAG
    CACCGAGTGG TGAGCGTCCG GGAGTGCGCC CGCTCCCAGG GCTTCCCCGA CACCTATCGG
    CTGTTCGGCA ACATCCTAGA CAAGCACCGG CAGGTGGGTA ATGCTGTGCC GCCGCCACTG
    GCCAAAGCCA TCGGCTTGGA GATCAAGCGC TGCATGTTGG CCAAAGCGCG CGAGAGCGCC
    TCAGCTGGAC TTTCTACTTG TATATTTGCC TATGTCATCA ACTGCCCAGA TCAAGATGCA
    GAAAATTCCC TTCACCTGCC TGGCACTGCA AGCTGGTTCC ATCTCCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235761
    Clone ID Related Accession (Same CDS sequence) XM_024993831.1 , XM_024993830.1
    Accession Version XM_024993830.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGGAGGAG
    AAGAGACGCA GAACTACATA CAGAGAACTA ACCGAGAAGA AAATGACTCG AACCAAAATA
    GCCGTAGTGT CCAAGACCAA TCCTCCGAAG TGCACCGAGT GCTTGCAGTA CCTGGACGAC
    CCTGAGCTGA GATACGAGCA GCACCCCCCC GATGCGGTGG AAGAGATACA GATACTGACC
    AACGAGAGGT TGTCCATCTT TGATGCCAAC GAATCTGGCT TTGAGAGTTA CGAGGATTTG
    CCTCAGCACA AACTAACCTG CTTCAGCGTG TACTGTAAAC GCGGTCACCT TTGCCCGATC
    GACACCGGCC TCATTGAGAA GGATGTCGAG CTCCTCTTTT CTGGTTCAGC AAAGCCGATA
    TATGAGGATG ACCCATCTCC CGAAGGTGGT ATTAATGGCA AAAATTTTGG CCCCATAAAC
    GAATGGTGGA TTGCTGGTTT TGATGGAGGT GAAAAGGCTC TTCTTGGCTT TAGCACCTCA
    TTTGCCGAGT ATATCTTGAT GGATCCCAGC CCAGAGTACG CACCACTATT CAGCGTGATG
    CAGGAGAAGA TCTATATAAG TAAGATAGTG GTTGAGTTCC TGCAGAGCAA CCCTGACTCC
    ACCTACGAAG ACCTGATCAA TAAGATTGAG ACCACCGTTC CTCCTTGTAT GCTCAACTTG
    AATCGATTCA CAGAGGATTC TCTCCTGCGG CATGCCCAGT TCGTGGTGGA GCAAGTAGAG
    AGTTATGATC GGGCTGGGGA CAGTGACGAG CAGCCCATCT TCCTGAGCCC CTGCATGAGA
    GACCTCATCA AGCTGGCCGG GGTCACCCTG GGAAAAAGGC GAGCCGAGAG GCGGCAGACC
    ATCCGGCAAC CCGCCAAAGA GAAGGACAAG GGCCCCACCA AGGCCACCAC CACCAAGCTG
    GTCTACCAGA TCTTTGACAC TTTCTTTGCG GAGCAAATTG AAAAAGATGA CAAGGAAGAC
    AAGGAGAATG CCTTCAAGCG CCGGCGCTGT GGCGTCTGTG AGATTTGTCA ACAGCCCGAG
    TGTGGAAAGT GTAAGGCCTG TAAGGATATG GTTAAATTTG GTGGTAGCGG ACGGAGCAAG
    CAGGCTTGCC AAAAGAGGAG GTGTCCCAAC ATGGCCATGA AGGAGGCAGA CGATGACGAG
    GAAGTGGATG ACAATATTCC AGAGATGCCA TCACCCAAAA AGATGCATCA GGGGAAGAAA
    AAGAAGCAGA ATAAGAATCG GATCTCTTGG GTTGGCGATG CCGTCAAGAC TGACGGGAAG
    AAGAGTTACT ACAAGAAGGT ATGCATCGAC TCGGAAACCC TGGAAGTGGG GGACTGTGTT
    TCTGTAATTC CAGACGACTC TTCAAAACCA CTGTATCTAG CAAGGGTCAC GGCGCTGTGG
    GAGGACAGCA GCAATGGGCA GATGTTCCAT GCCCACTGGT TCTGTGCTGG GACGGACACG
    GTCCTCGGGG CCACATCGGA CCCCCTGGAG CTGTTCCTGG TTGACGAGTG TGAGGACATG
    CAGCTCTCGT ACATCCACAG CAAGGTGCAG GTCATTTATA AGGCGCCCTC AGAGAACTGG
    GCCATGGAGG GAGGCGTGGA CCCCGAGGCC CTGATGTCAG AGGACGACGG GAAGACCTAC
    TTCTACCAGC TGTGGTACGA CCAAGACTAC GCGAGATTTG AGTCCCCTCC GAAAACTCAG
    CCGACGGAGG ACAACAAGTA CAAGTTCTGC GCAAGCTGTG CACGTCTGGC CGAAATGAGG
    CAGAAGGAAA TCCCCAGGGT CGTGGAGCAG CTCCAGGACC TGGAAGGCCG CGTCCTCTAC
    AGCCTCGCCA CCAAGAACGG CGTCCAGTAC CGGGTGGGCG ATGGCGTGTA CCTCCCTCCC
    GAGGCCTTCA CCTTCAACAT CAAGCTGTCC AGTCCTGTGA AACGCCCCCG GAAGGAGCCT
    GTGGACGAAG CTCTGTATCC AGAACACTAC CGGAAGTACT CTGACTACAT CAAGGGCAGC
    AACCTGGATG CCCCTGAGCC CTACCGTATT GGCCGCATAA AGGAGATCTT CTGCAGCAAG
    AAGAGCAACG GCCGGCCCAA TGAGACAGAC ATCAAGATCA GGGTCAACAA GTTCTACAGG
    CCGGAGAACA CACACAAGTC TACCCCAGCC AGTTACCACG CAGACATCAA CCTGCTTTAC
    TGGAGCGATG AGGAGGCCGT GGTGGACTTC AAGGCCGTGC AGGGCCGCTG CACCGTGGAG
    TACGGAGAGG ACCTGCCTCA GTGCCTCCAG GACTTCTCCG CTGGTGGCCC CGATCGCTTC
    TATTTTCTCG AGGCCTATAA CGCCAAGAGC AAAAGCTTTG AAGATCCTCC GAACCACGCC
    CGGAGCACCG GAAATAAAGG GAAAGGGAAG GGGAAAGGAA AAAACAGGAC GAAATCTCAG
    ACGTGTGAGC CGAGTGAACT GGAGACAGAA ATCAAACTGC CGAAGCTGCG GACCCTGGAC
    GTGTTTTCCG GCTGTGGGGG ATTGTCGGAA GGCTTCCACC AAGCAGGCAT CTCGGAGACA
    CTTTGGGCCA TCGAGATGTG GGACCCTGCG GCCCAGGCGT TCCGGCTCAA CAACCCTGGG
    TCCACGGTGT TCACAGAGGA CTGCAACGTC CTGCTGAAGC TGGTCATGGC TGGGGAGGTG
    ACCAACTCCC GCGGCCAGAA GCTGCCTCAG AAGGGAGATG TGGAGATGCT GTGCGGCGGG
    CCGCCCTGCC AGGGCTTCAG CGGCATGAAC CGCTTCAACT CTCGAACCTA CTCCAAATTC
    AAGAACTCTC TGGTGGTCTC TTTCCTCAGC TACTGTGACT ACTACCGGCC CCGCTACTTC
    CTCTTGGAGA ACGTTCGGAA CTTCGTCTCC TTCAAGCGCT CCATGGTCCT GAAGCTGACG
    CTGCGCTGCC TGGTCCGCAT GGGCTACCAG TGCACCTTTG GCGTGCTGCA GGCTGGTCAG
    TACGGCGTGG CCCAGACTCG GAGGCGAGCC ATCATCCTGG CTGCAGCCCC TGGGGAGCCA
    CTCCCGCTGT TCCCGGAGCC GTTGCATGTG TTCGCACCCC GGGCCTGCCA GCTGAGCGTC
    GTAGTGGACG ACAAGAAGTT TGTCAGCAAC ATCACCAGGT TGAGCTCGGG TCCCTTCCGA
    ACCATCACCG TGCGGGACAC CATGTCTGAC CTCCCTGAGA TCCGGAACGG GGCCTCGGCA
    CTGGAGATTT CATACAACGG GGAGCCCCAG TCCTGGTTCC AGAGGCAGCT CCGGGGCTCG
    CAGTACCAGC CCATCCTCAG GGATCATATT TGCAAGGACA TGAGCGCCTT GGTGGCTGCC
    CGCATGCGGC ACATCCCCCT GGCCCCGGGC TCGGACTGGC GTGACCTGCC CAACATTGAG
    GTGCGGCTCT CTGACGGCAC CCTGGCCCGG AAGCTGCGGT ACAACTACCA CGACAAGAAG
    AACGGCTGCA GCAGCAGCGG CGCCCTCCGT GGGGTCTGCT CCTGTGTGGA AGGCAAGCCC
    TGTGAGCCTG CGGCCCGACA GTTTAACACC CTTATCCCCT GGTGCCTGCC CCACACTGGG
    AACAGGCACA ACCACTGGGC CGGCCTCTAC GGGCGTCTCG AGTGGGACGG CTTCTTCAGC
    ACAACTGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCCGAGCAG
    CACCGAGTGG TGAGCGTCCG GGAGTGCGCC CGCTCCCAGG GCTTCCCCGA CACCTATCGG
    CTGTTCGGCA ACATCCTAGA CAAGCACCGG CAGGTGGGTA ATGCTGTGCC GCCGCCACTG
    GCCAAAGCCA TCGGCTTGGA GATCAAGCGC TGCATGTTGG CCAAAGCGCG CGAGAGCGCC
    TCAGCTGGAC TTTCTACTTG TATATTTGCC TATGTCATCA ACTGCCCAGA TCAAGATGCA
    GAAAATTCCC TTCACCTGCC TGGCACTGCA AGCTGGTTCC ATCTCCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849598.1
    CDS79..4989
    Translation

    Target ORF information:

    RefSeq Version XM_024993830.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993830.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGGAGGAG
    AAGAGACGCA GAACTACATA CAGAGAACTA ACCGAGAAGA AAATGACTCG AACCAAAATA
    GCCGTAGTGT CCAAGACCAA TCCTCCGAAG TGCACCGAGT GCTTGCAGTA CCTGGACGAC
    CCTGAGCTGA GATACGAGCA GCACCCCCCC GATGCGGTGG AAGAGATACA GATACTGACC
    AACGAGAGGT TGTCCATCTT TGATGCCAAC GAATCTGGCT TTGAGAGTTA CGAGGATTTG
    CCTCAGCACA AACTAACCTG CTTCAGCGTG TACTGTAAAC GCGGTCACCT TTGCCCGATC
    GACACCGGCC TCATTGAGAA GGATGTCGAG CTCCTCTTTT CTGGTTCAGC AAAGCCGATA
    TATGAGGATG ACCCATCTCC CGAAGGTGGT ATTAATGGCA AAAATTTTGG CCCCATAAAC
    GAATGGTGGA TTGCTGGTTT TGATGGAGGT GAAAAGGCTC TTCTTGGCTT TAGCACCTCA
    TTTGCCGAGT ATATCTTGAT GGATCCCAGC CCAGAGTACG CACCACTATT CAGCGTGATG
    CAGGAGAAGA TCTATATAAG TAAGATAGTG GTTGAGTTCC TGCAGAGCAA CCCTGACTCC
    ACCTACGAAG ACCTGATCAA TAAGATTGAG ACCACCGTTC CTCCTTGTAT GCTCAACTTG
    AATCGATTCA CAGAGGATTC TCTCCTGCGG CATGCCCAGT TCGTGGTGGA GCAAGTAGAG
    AGTTATGATC GGGCTGGGGA CAGTGACGAG CAGCCCATCT TCCTGAGCCC CTGCATGAGA
    GACCTCATCA AGCTGGCCGG GGTCACCCTG GGAAAAAGGC GAGCCGAGAG GCGGCAGACC
    ATCCGGCAAC CCGCCAAAGA GAAGGACAAG GGCCCCACCA AGGCCACCAC CACCAAGCTG
    GTCTACCAGA TCTTTGACAC TTTCTTTGCG GAGCAAATTG AAAAAGATGA CAAGGAAGAC
    AAGGAGAATG CCTTCAAGCG CCGGCGCTGT GGCGTCTGTG AGATTTGTCA ACAGCCCGAG
    TGTGGAAAGT GTAAGGCCTG TAAGGATATG GTTAAATTTG GTGGTAGCGG ACGGAGCAAG
    CAGGCTTGCC AAAAGAGGAG GTGTCCCAAC ATGGCCATGA AGGAGGCAGA CGATGACGAG
    GAAGTGGATG ACAATATTCC AGAGATGCCA TCACCCAAAA AGATGCATCA GGGGAAGAAA
    AAGAAGCAGA ATAAGAATCG GATCTCTTGG GTTGGCGATG CCGTCAAGAC TGACGGGAAG
    AAGAGTTACT ACAAGAAGGT ATGCATCGAC TCGGAAACCC TGGAAGTGGG GGACTGTGTT
    TCTGTAATTC CAGACGACTC TTCAAAACCA CTGTATCTAG CAAGGGTCAC GGCGCTGTGG
    GAGGACAGCA GCAATGGGCA GATGTTCCAT GCCCACTGGT TCTGTGCTGG GACGGACACG
    GTCCTCGGGG CCACATCGGA CCCCCTGGAG CTGTTCCTGG TTGACGAGTG TGAGGACATG
    CAGCTCTCGT ACATCCACAG CAAGGTGCAG GTCATTTATA AGGCGCCCTC AGAGAACTGG
    GCCATGGAGG GAGGCGTGGA CCCCGAGGCC CTGATGTCAG AGGACGACGG GAAGACCTAC
    TTCTACCAGC TGTGGTACGA CCAAGACTAC GCGAGATTTG AGTCCCCTCC GAAAACTCAG
    CCGACGGAGG ACAACAAGTA CAAGTTCTGC GCAAGCTGTG CACGTCTGGC CGAAATGAGG
    CAGAAGGAAA TCCCCAGGGT CGTGGAGCAG CTCCAGGACC TGGAAGGCCG CGTCCTCTAC
    AGCCTCGCCA CCAAGAACGG CGTCCAGTAC CGGGTGGGCG ATGGCGTGTA CCTCCCTCCC
    GAGGCCTTCA CCTTCAACAT CAAGCTGTCC AGTCCTGTGA AACGCCCCCG GAAGGAGCCT
    GTGGACGAAG CTCTGTATCC AGAACACTAC CGGAAGTACT CTGACTACAT CAAGGGCAGC
    AACCTGGATG CCCCTGAGCC CTACCGTATT GGCCGCATAA AGGAGATCTT CTGCAGCAAG
    AAGAGCAACG GCCGGCCCAA TGAGACAGAC ATCAAGATCA GGGTCAACAA GTTCTACAGG
    CCGGAGAACA CACACAAGTC TACCCCAGCC AGTTACCACG CAGACATCAA CCTGCTTTAC
    TGGAGCGATG AGGAGGCCGT GGTGGACTTC AAGGCCGTGC AGGGCCGCTG CACCGTGGAG
    TACGGAGAGG ACCTGCCTCA GTGCCTCCAG GACTTCTCCG CTGGTGGCCC CGATCGCTTC
    TATTTTCTCG AGGCCTATAA CGCCAAGAGC AAAAGCTTTG AAGATCCTCC GAACCACGCC
    CGGAGCACCG GAAATAAAGG GAAAGGGAAG GGGAAAGGAA AAAACAGGAC GAAATCTCAG
    ACGTGTGAGC CGAGTGAACT GGAGACAGAA ATCAAACTGC CGAAGCTGCG GACCCTGGAC
    GTGTTTTCCG GCTGTGGGGG ATTGTCGGAA GGCTTCCACC AAGCAGGCAT CTCGGAGACA
    CTTTGGGCCA TCGAGATGTG GGACCCTGCG GCCCAGGCGT TCCGGCTCAA CAACCCTGGG
    TCCACGGTGT TCACAGAGGA CTGCAACGTC CTGCTGAAGC TGGTCATGGC TGGGGAGGTG
    ACCAACTCCC GCGGCCAGAA GCTGCCTCAG AAGGGAGATG TGGAGATGCT GTGCGGCGGG
    CCGCCCTGCC AGGGCTTCAG CGGCATGAAC CGCTTCAACT CTCGAACCTA CTCCAAATTC
    AAGAACTCTC TGGTGGTCTC TTTCCTCAGC TACTGTGACT ACTACCGGCC CCGCTACTTC
    CTCTTGGAGA ACGTTCGGAA CTTCGTCTCC TTCAAGCGCT CCATGGTCCT GAAGCTGACG
    CTGCGCTGCC TGGTCCGCAT GGGCTACCAG TGCACCTTTG GCGTGCTGCA GGCTGGTCAG
    TACGGCGTGG CCCAGACTCG GAGGCGAGCC ATCATCCTGG CTGCAGCCCC TGGGGAGCCA
    CTCCCGCTGT TCCCGGAGCC GTTGCATGTG TTCGCACCCC GGGCCTGCCA GCTGAGCGTC
    GTAGTGGACG ACAAGAAGTT TGTCAGCAAC ATCACCAGGT TGAGCTCGGG TCCCTTCCGA
    ACCATCACCG TGCGGGACAC CATGTCTGAC CTCCCTGAGA TCCGGAACGG GGCCTCGGCA
    CTGGAGATTT CATACAACGG GGAGCCCCAG TCCTGGTTCC AGAGGCAGCT CCGGGGCTCG
    CAGTACCAGC CCATCCTCAG GGATCATATT TGCAAGGACA TGAGCGCCTT GGTGGCTGCC
    CGCATGCGGC ACATCCCCCT GGCCCCGGGC TCGGACTGGC GTGACCTGCC CAACATTGAG
    GTGCGGCTCT CTGACGGCAC CCTGGCCCGG AAGCTGCGGT ACAACTACCA CGACAAGAAG
    AACGGCTGCA GCAGCAGCGG CGCCCTCCGT GGGGTCTGCT CCTGTGTGGA AGGCAAGCCC
    TGTGAGCCTG CGGCCCGACA GTTTAACACC CTTATCCCCT GGTGCCTGCC CCACACTGGG
    AACAGGCACA ACCACTGGGC CGGCCTCTAC GGGCGTCTCG AGTGGGACGG CTTCTTCAGC
    ACAACTGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCCGAGCAG
    CACCGAGTGG TGAGCGTCCG GGAGTGCGCC CGCTCCCAGG GCTTCCCCGA CACCTATCGG
    CTGTTCGGCA ACATCCTAGA CAAGCACCGG CAGGTGGGTA ATGCTGTGCC GCCGCCACTG
    GCCAAAGCCA TCGGCTTGGA GATCAAGCGC TGCATGTTGG CCAAAGCGCG CGAGAGCGCC
    TCAGCTGGAC TTTCTACTTG TATATTTGCC TATGTCATCA ACTGCCCAGA TCAAGATGCA
    GAAAATTCCC TTCACCTGCC TGGCACTGCA AGCTGGTTCC ATCTCCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235762
    Clone ID Related Accession (Same CDS sequence) XM_024993832.1
    Accession Version XM_024993832.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGGAGGAG
    AAGAGACGCA GAACTACATA CAGAGAACTA ACCGAGAAGA AAATGACTCG AACCAAAATA
    GCCGTAGTGT CCAAGACCAA TCCTCCGAAG TGCACCGAGT GCTTGCAGTA CCTGGACGAC
    CCTGAGCTGA GATACGAGCA GCACCCCCCC GATGCGGTGG AAGAGATACA GATACTGACC
    AACGAGAGGT TGTCCATCTT TGATGCCAAC GAATCTGGCT TTGAGAGTTA CGAGGATTTG
    CCTCAGCACA AACTAACCTG CTTCAGCGTG TACTGTAAAC GCGGTCACCT TTGCCCGATC
    GACACCGGCC TCATTGAGAA GGATGTCGAG CTCCTCTTTT CTGGTTCAGC AAAGCCGATA
    TATGAGGATG ACCCATCTCC CGAAGGTGGT ATTAATGGCA AAAATTTTGG CCCCATAAAC
    GAATGGTGGA TTGCTGGTTT TGATGGAGGT GAAAAGGCTC TTCTTGGCTT TAGCACCTCA
    TTTGCCGAGT ATATCTTGAT GGATCCCAGC CCAGAGTACG CACCACTATT CAGCGTGATG
    CAGGAGAAGA TCTATATAAG TAAGATAGTG GTTGAGTTCC TGCAGAGCAA CCCTGACTCC
    ACCTACGAAG ACCTGATCAA TAAGATTGAG ACCACCGTTC CTCCTTGTAT GCTCAACTTG
    AATCGATTCA CAGAGGATTC TCTCCTGCGG CATGCCCAGT TCGTGGTGGA GCAAGTAGAG
    AGTTATGATC GGGCTGGGGA CAGTGACGAG CAGCCCATCT TCCTGAGCCC CTGCATGAGA
    GACCTCATCA AGCTGGCCGG GGTCACCCTG GGAAAAAGGC GAGCCGAGAG GCGGCAGACC
    ATCCGGCAAC CCGCCAAAGA GAAGGACAAG GGCCCCACCA AGGCCACCAC CACCAAGCTG
    GTCTACCAGA TCTTTGACAC TTTCTTTGCG GAGCAAATTG AAAAAGATGA CAAGGAAGAC
    AAGGAGAATG CCTTCAAGCG CCGGCGCTGT GGCGTCTGTG AGATTTGTCA ACAGCCCGAG
    TGTGGAAAGT GTAAGGCCTG TAAGGATATG GTTAAATTTG GTGGTAGCGG ACGGAGCAAG
    CAGGCTTGCC AAAAGAGGAG GTGTCCCAAC ATGGCCATGA AGGAGGCAGA CGATGACGAG
    GAAGTGGATG ACAATATTCC AGAGATGCCA TCACCCAAAA AGATGCATCA GGGGAAGAAA
    AAGAAGCAGA ATAAGAATCG GATCTCTTGG GTTGGCGATG CCGTCAAGAC TGACGGGAAG
    AAGAGTTACT ACAAGAAGGT ATGCATCGAC TCGGAAACCC TGGAAGTGGG GGACTGTGTT
    TCTGTAATTC CAGACGACTC TTCAAAACCA CTGTATCTAG CAAGGGTCAC GGCGCTGTGG
    GAGGACAGCA GCAATGGGCA GATGTTCCAT GCCCACTGGT TCTGTGCTGG GACGGACACG
    GTCCTCGGGG CCACATCGGA CCCCCTGGAG CTGTTCCTGG TTGACGAGTG TGAGGACATG
    CAGCTCTCGT ACATCCACAG CAAGGTGCAG GTCATTTATA AGGCGCCCTC AGAGAACTGG
    GCCATGGAGG GAGGCGTGGA CCCCGAGGCC CTGATGTCAG AGGACGACGG GAAGACCTAC
    TTCTACCAGC TGTGGTACGA CCAAGACTAC GCGAGATTTG AGTCCCCTCC GAAAACTCAG
    CCGACGGAGG ACAACAAGTA CAAGTTCTGC GCAAGCTGTG CACGTCTGGC CGAAATGAGG
    CAGAAGGAAA TCCCCAGGGT CGTGGAGCAG CTCCAGGACC TGGAAGGCCG CGTCCTCTAC
    AGCCTCGCCA CCAAGAACGG CGTCCAGTAC CGGGTGGGCG ATGGCGTGTA CCTCCCTCCC
    GAGGCCTTCA CCTTCAACAT CAAGCTGTCC AGTCCTGTGA AACGCCCCCG GAAGGAGCCT
    GTGGACGAAG CTCTGTATCC AGAACACTAC CGGAAGTACT CTGACTACAT CAAGGGCAGC
    AACCTGGATG CCCCTGAGCC CTACCGTATT GGCCGCATAA AGGAGATCTT CTGCAGCAAG
    AAGAGCAACG GCCGGCCCAA TGAGACAGAC ATCAAGATCA GGGTCAACAA GTTCTACAGG
    CCGGAGAACA CACACAAGTC TACCCCAGCC AGTTACCACG CAGACATCAA CCTGCTTTAC
    TGGAGCGATG AGGAGGCCGT GGTGGACTTC AAGGCCGTGC AGGGCCGCTG CACCGTGGAG
    TACGGAGAGG ACCTGCCTCA GTGCCTCCAG GACTTCTCCG CTGGTGGCCC CGATCGCTTC
    TATTTTCTCG AGGCCTATAA CGCCAAGAGC AAAAGCTTTG AAGATCCTCC GAACCACGCC
    CGGAGCACCG GAAATAAAGG GAAAGGGAAG GGGAAAGGAA AAAACAGGAC GAAATCTCAG
    ACGTGTGAGC CGAGTGAACT GGAGACAGAA ATCAAACTGC CGAAGCTGCG GACCCTGGAC
    GTGTTTTCCG GCTGTGGGGG ATTGTCGGAA GGCTTCCACC AAGCAGGCAT CTCGGAGACA
    CTTTGGGCCA TCGAGATGTG GGACCCTGCG GCCCAGGCGT TCCGGCTCAA CAACCCTGGG
    TCCACGGTGT TCACAGAGGA CTGCAACGTC CTGCTGAAGC TGGTCATGGC TGGGGAGGTG
    ACCAACTCCC GCGGCCAGAA GCTGCCTCAG AAGGGAGATG TGGAGATGCT GTGCGGCGGG
    CCGCCCTGCC AGGGCTTCAG CGGCATGAAC CGCTTCAACT CTCGAACCTA CTCCAAATTC
    AAGAACTCTC TGGTGGTCTC TTTCCTCAGC TACTGTGACT ACTACCGGCC CCGCTACTTC
    CTCTTGGAGA ACGTTCGGAA CTTCGTCTCC TTCAAGCGCT CCATGGTCCT GAAGCTGACG
    CTGCGCTGCC TGGTCCGCAT GGGCTACCAG TGCACCTTTG GCGTGCTGCA GGCTGGTCAG
    TACGGCGTGG CCCAGACTCG GAGGCGAGCC ATCATCCTGG CTGCAGCCCC TGGGGAGCCA
    CTCCCGCTGT TCCCGGAGCC GTTGCATGTG TTCGCACCCC GGGCCTGCCA GCTGAGCGTC
    GTAGTGGACG ACAAGAAGTT TGTCAGCAAC ATCACCAGGT TGAGCTCGGG TCCCTTCCGA
    ACCATCACCG TGCGGGACAC CATGTCTGAC CTCCCTGAGA TCCGGAACGG GGCCTCGGCA
    CTGGAGATTT CATACAACGG GGAGCCCCAG TCCTGGTTCC AGAGGCAGCT CCGGGGCTCG
    CAGTACCAGC CCATCCTCAG GGATCATATT TGCAAGGACA TGAGCGCCTT GGTGGCTGCC
    CGCATGCGGC ACATCCCCCT GGCCCCGGGC TCGGACTGGC GTGACCTGCC CAACATTGAG
    GTGCGGCTCT CTGACGGCAC CCTGGCCCGG AAGCTGCGGT ACAACTACCA CGACAAGAAG
    AACGGCTGCA GCAGCAGCGG CGCCCTCCGT GGGGTCTGCT CCTGTGTGGA AGGCAAGCCC
    TGTGAGCCTG CGGCCCGACA GTTTAACACC CTTATCCCCT GGTGCCTGCC CCACACTGGG
    AACAGGCACA ACCACTGGGC CGGCCTCTAC GGGCGTCTCG AGTGGGACGG CTTCTTCAGC
    ACAACTGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCCGAGCAG
    CACCGAGTGG TGAGCGTCCG GGAGTGCGCC CGCTCCCAGG GCTTCCCCGA CACCTATCGG
    CTGTTCGGCA ACATCCTAGA CAAGCACCGG CAGGTGGGTA ATGCTGTGCC GCCGCCACTG
    GCCAAAGCCA TCGGCTTGGA GATCAAGCGC TGCATGTTGG CCAAAGCGCG CGAGAGCGCC
    TCAGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849600.1
    CDS79..4887
    Translation

    Target ORF information:

    RefSeq Version XM_024993832.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993832.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGGAGGAG
    AAGAGACGCA GAACTACATA CAGAGAACTA ACCGAGAAGA AAATGACTCG AACCAAAATA
    GCCGTAGTGT CCAAGACCAA TCCTCCGAAG TGCACCGAGT GCTTGCAGTA CCTGGACGAC
    CCTGAGCTGA GATACGAGCA GCACCCCCCC GATGCGGTGG AAGAGATACA GATACTGACC
    AACGAGAGGT TGTCCATCTT TGATGCCAAC GAATCTGGCT TTGAGAGTTA CGAGGATTTG
    CCTCAGCACA AACTAACCTG CTTCAGCGTG TACTGTAAAC GCGGTCACCT TTGCCCGATC
    GACACCGGCC TCATTGAGAA GGATGTCGAG CTCCTCTTTT CTGGTTCAGC AAAGCCGATA
    TATGAGGATG ACCCATCTCC CGAAGGTGGT ATTAATGGCA AAAATTTTGG CCCCATAAAC
    GAATGGTGGA TTGCTGGTTT TGATGGAGGT GAAAAGGCTC TTCTTGGCTT TAGCACCTCA
    TTTGCCGAGT ATATCTTGAT GGATCCCAGC CCAGAGTACG CACCACTATT CAGCGTGATG
    CAGGAGAAGA TCTATATAAG TAAGATAGTG GTTGAGTTCC TGCAGAGCAA CCCTGACTCC
    ACCTACGAAG ACCTGATCAA TAAGATTGAG ACCACCGTTC CTCCTTGTAT GCTCAACTTG
    AATCGATTCA CAGAGGATTC TCTCCTGCGG CATGCCCAGT TCGTGGTGGA GCAAGTAGAG
    AGTTATGATC GGGCTGGGGA CAGTGACGAG CAGCCCATCT TCCTGAGCCC CTGCATGAGA
    GACCTCATCA AGCTGGCCGG GGTCACCCTG GGAAAAAGGC GAGCCGAGAG GCGGCAGACC
    ATCCGGCAAC CCGCCAAAGA GAAGGACAAG GGCCCCACCA AGGCCACCAC CACCAAGCTG
    GTCTACCAGA TCTTTGACAC TTTCTTTGCG GAGCAAATTG AAAAAGATGA CAAGGAAGAC
    AAGGAGAATG CCTTCAAGCG CCGGCGCTGT GGCGTCTGTG AGATTTGTCA ACAGCCCGAG
    TGTGGAAAGT GTAAGGCCTG TAAGGATATG GTTAAATTTG GTGGTAGCGG ACGGAGCAAG
    CAGGCTTGCC AAAAGAGGAG GTGTCCCAAC ATGGCCATGA AGGAGGCAGA CGATGACGAG
    GAAGTGGATG ACAATATTCC AGAGATGCCA TCACCCAAAA AGATGCATCA GGGGAAGAAA
    AAGAAGCAGA ATAAGAATCG GATCTCTTGG GTTGGCGATG CCGTCAAGAC TGACGGGAAG
    AAGAGTTACT ACAAGAAGGT ATGCATCGAC TCGGAAACCC TGGAAGTGGG GGACTGTGTT
    TCTGTAATTC CAGACGACTC TTCAAAACCA CTGTATCTAG CAAGGGTCAC GGCGCTGTGG
    GAGGACAGCA GCAATGGGCA GATGTTCCAT GCCCACTGGT TCTGTGCTGG GACGGACACG
    GTCCTCGGGG CCACATCGGA CCCCCTGGAG CTGTTCCTGG TTGACGAGTG TGAGGACATG
    CAGCTCTCGT ACATCCACAG CAAGGTGCAG GTCATTTATA AGGCGCCCTC AGAGAACTGG
    GCCATGGAGG GAGGCGTGGA CCCCGAGGCC CTGATGTCAG AGGACGACGG GAAGACCTAC
    TTCTACCAGC TGTGGTACGA CCAAGACTAC GCGAGATTTG AGTCCCCTCC GAAAACTCAG
    CCGACGGAGG ACAACAAGTA CAAGTTCTGC GCAAGCTGTG CACGTCTGGC CGAAATGAGG
    CAGAAGGAAA TCCCCAGGGT CGTGGAGCAG CTCCAGGACC TGGAAGGCCG CGTCCTCTAC
    AGCCTCGCCA CCAAGAACGG CGTCCAGTAC CGGGTGGGCG ATGGCGTGTA CCTCCCTCCC
    GAGGCCTTCA CCTTCAACAT CAAGCTGTCC AGTCCTGTGA AACGCCCCCG GAAGGAGCCT
    GTGGACGAAG CTCTGTATCC AGAACACTAC CGGAAGTACT CTGACTACAT CAAGGGCAGC
    AACCTGGATG CCCCTGAGCC CTACCGTATT GGCCGCATAA AGGAGATCTT CTGCAGCAAG
    AAGAGCAACG GCCGGCCCAA TGAGACAGAC ATCAAGATCA GGGTCAACAA GTTCTACAGG
    CCGGAGAACA CACACAAGTC TACCCCAGCC AGTTACCACG CAGACATCAA CCTGCTTTAC
    TGGAGCGATG AGGAGGCCGT GGTGGACTTC AAGGCCGTGC AGGGCCGCTG CACCGTGGAG
    TACGGAGAGG ACCTGCCTCA GTGCCTCCAG GACTTCTCCG CTGGTGGCCC CGATCGCTTC
    TATTTTCTCG AGGCCTATAA CGCCAAGAGC AAAAGCTTTG AAGATCCTCC GAACCACGCC
    CGGAGCACCG GAAATAAAGG GAAAGGGAAG GGGAAAGGAA AAAACAGGAC GAAATCTCAG
    ACGTGTGAGC CGAGTGAACT GGAGACAGAA ATCAAACTGC CGAAGCTGCG GACCCTGGAC
    GTGTTTTCCG GCTGTGGGGG ATTGTCGGAA GGCTTCCACC AAGCAGGCAT CTCGGAGACA
    CTTTGGGCCA TCGAGATGTG GGACCCTGCG GCCCAGGCGT TCCGGCTCAA CAACCCTGGG
    TCCACGGTGT TCACAGAGGA CTGCAACGTC CTGCTGAAGC TGGTCATGGC TGGGGAGGTG
    ACCAACTCCC GCGGCCAGAA GCTGCCTCAG AAGGGAGATG TGGAGATGCT GTGCGGCGGG
    CCGCCCTGCC AGGGCTTCAG CGGCATGAAC CGCTTCAACT CTCGAACCTA CTCCAAATTC
    AAGAACTCTC TGGTGGTCTC TTTCCTCAGC TACTGTGACT ACTACCGGCC CCGCTACTTC
    CTCTTGGAGA ACGTTCGGAA CTTCGTCTCC TTCAAGCGCT CCATGGTCCT GAAGCTGACG
    CTGCGCTGCC TGGTCCGCAT GGGCTACCAG TGCACCTTTG GCGTGCTGCA GGCTGGTCAG
    TACGGCGTGG CCCAGACTCG GAGGCGAGCC ATCATCCTGG CTGCAGCCCC TGGGGAGCCA
    CTCCCGCTGT TCCCGGAGCC GTTGCATGTG TTCGCACCCC GGGCCTGCCA GCTGAGCGTC
    GTAGTGGACG ACAAGAAGTT TGTCAGCAAC ATCACCAGGT TGAGCTCGGG TCCCTTCCGA
    ACCATCACCG TGCGGGACAC CATGTCTGAC CTCCCTGAGA TCCGGAACGG GGCCTCGGCA
    CTGGAGATTT CATACAACGG GGAGCCCCAG TCCTGGTTCC AGAGGCAGCT CCGGGGCTCG
    CAGTACCAGC CCATCCTCAG GGATCATATT TGCAAGGACA TGAGCGCCTT GGTGGCTGCC
    CGCATGCGGC ACATCCCCCT GGCCCCGGGC TCGGACTGGC GTGACCTGCC CAACATTGAG
    GTGCGGCTCT CTGACGGCAC CCTGGCCCGG AAGCTGCGGT ACAACTACCA CGACAAGAAG
    AACGGCTGCA GCAGCAGCGG CGCCCTCCGT GGGGTCTGCT CCTGTGTGGA AGGCAAGCCC
    TGTGAGCCTG CGGCCCGACA GTTTAACACC CTTATCCCCT GGTGCCTGCC CCACACTGGG
    AACAGGCACA ACCACTGGGC CGGCCTCTAC GGGCGTCTCG AGTGGGACGG CTTCTTCAGC
    ACAACTGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCCGAGCAG
    CACCGAGTGG TGAGCGTCCG GGAGTGCGCC CGCTCCCAGG GCTTCCCCGA CACCTATCGG
    CTGTTCGGCA ACATCCTAGA CAAGCACCGG CAGGTGGGTA ATGCTGTGCC GCCGCCACTG
    GCCAAAGCCA TCGGCTTGGA GATCAAGCGC TGCATGTTGG CCAAAGCGCG CGAGAGCGCC
    TCAGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91052
    Clone ID Related Accession (Same CDS sequence) XM_015471991.2 , XM_015471991.1
    Accession Version XM_015471991.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGGGCATAT AAAAGTCATG
    TTTGGGGGAT TCCTTGATGG CTTAGTGGTA AAGAATCTGC CTGCCAGTGC AGGAGACACG
    GATTCAATCC CTGATCCAGG AAGATCCCAC ATGCTGCAGA GCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327477.1
    CDS79..5046
    Translation

    Target ORF information:

    RefSeq Version XM_015471991.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471991.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGGGCATAT AAAAGTCATG
    TTTGGGGGAT TCCTTGATGG CTTAGTGGTA AAGAATCTGC CTGCCAGTGC AGGAGACACG
    GATTCAATCC CTGATCCAGG AAGATCCCAC ATGCTGCAGA GCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91052
    Clone ID Related Accession (Same CDS sequence) XM_015471991.2 , XM_015471991.1
    Accession Version XM_015471991.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGGGCATAT AAAAGTCATG
    TTTGGGGGAT TCCTTGATGG CTTAGTGGTA AAGAATCTGC CTGCCAGTGC AGGAGACACG
    GATTCAATCC CTGATCCAGG AAGATCCCAC ATGCTGCAGA GCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327477.1
    CDS76..5043
    Translation

    Target ORF information:

    RefSeq Version XM_015471991.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471991.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGGGCATAT AAAAGTCATG
    TTTGGGGGAT TCCTTGATGG CTTAGTGGTA AAGAATCTGC CTGCCAGTGC AGGAGACACG
    GATTCAATCC CTGATCCAGG AAGATCCCAC ATGCTGCAGA GCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91053
    Clone ID Related Accession (Same CDS sequence) XM_015471992.1 , XM_015471992.2
    Accession Version XM_015471992.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGGAGGAG
    AAGAGACGCA GAACTACATA CAGAGAACTA ACCGAGAAGA AAATGACTCG AACCAAAATA
    GCCGTAGTGT CCAAGACCAA TCCTCCGAAG TGCACCGAGT GCTTGCAGTA CCTGGACGAC
    CCTGAGCTGA GATACGAGCA GCACCCCCCC GATGCGGTGG AAGAGATACA GATACTGACC
    AACGAGAGGT TGTCCATCTT TGATGCCAAC GAATCTGGCT TTGAGAGTTA CGAGGATTTG
    CCTCAGCACA AACTAACCTG CTTCAGCGTG TACTGTAAAC GCGGTCACCT TTGCCCGATC
    GACACCGGCC TCATTGAGAA GGATGTCGAG CTCCTCTTTT CTGGTTCAGC AAAGCCGATA
    TATGAGGATG ACCCATCTCC CGAAGGTGGT ATTAATGGCA AAAATTTTGG CCCCATAAAC
    GAATGGTGGA TTGCTGGTTT TGATGGAGGT GAAAAGGCTC TTCTTGGCTT TAGCACCTCA
    TTTGCCGAGT ATATCTTGAT GGATCCCAGC CCAGAGTACG CACCACTATT CAGCGTGATG
    CAGGAGAAGA TCTATATAAG TAAGATAGTG GTTGAGTTCC TGCAGAGCAA CCCTGACTCC
    ACCTACGAAG ACCTGATCAA TAAGATTGAG ACCACCGTTC CTCCTTGTAT GCTCAACTTG
    AATCGATTCA CAGAGGATTC TCTCCTGCGG CATGCCCAGT TCGTGGTGGA GCAAGTAGAG
    AGTTATGATC GGGCTGGGGA CAGTGACGAG CAGCCCATCT TCCTGAGCCC CTGCATGAGA
    GACCTCATCA AGCTGGCCGG GGTCACCCTG GGAAAAAGGC GAGCCGAGAG GCGGCAGACC
    ATCCGGCAAC CCGCCAAAGA GAAGGACAAG GGCCCCACCA AGGCCACCAC CACCAAGCTG
    GTCTACCAGA TCTTTGACAC TTTCTTTGCG GAGCAAATTG AAAAAGATGA CAAGGAAGAC
    AAGGAGAATG CCTTCAAGCG CCGGCGCTGT GGCGTCTGTG AGATTTGTCA ACAGCCCGAG
    TGTGGAAAGT GTAAGGCCTG TAAGGATATG GTTAAATTTG GTGGTAGCGG ACGGAGCAAG
    CAGGCTTGCC AAAAGAGGAG GTGTCCCAAC ATGGCCATGA AGGAGGCAGA CGATGACGAG
    GAAGTGGATG ACAATATTCC AGAGATGCCA TCACCCAAAA AGATGCATCA GGGGAAGAAA
    AAGAAGCAGA ATAAGAATCG GATCTCTTGG GTTGGCGATG CCGTCAAGAC TGACGGGAAG
    AAGAGTTACT ACAAGAAGGT ATGCATCGAC TCGGAAACCC TGGAAGTGGG GGACTGTGTT
    TCTGTAATTC CAGACGACTC TTCAAAACCA CTGTATCTAG CAAGGGTCAC GGCGCTGTGG
    GAGGACAGCA GCAATGGGCA GATGTTCCAT GCCCACTGGT TCTGTGCTGG GACGGACACG
    GTCCTCGGGG CCACATCGGA CCCCCTGGAG CTGTTCCTGG TTGACGAGTG TGAGGACATG
    CAGCTCTCGT ACATCCACAG CAAGGTGCAG GTCATTTATA AGGCGCCCTC AGAGAACTGG
    GCCATGGAGG GAGGCGTGGA CCCCGAGGCC CTGATGTCAG AGGACGACGG GAAGACCTAC
    TTCTACCAGC TGTGGTACGA CCAAGACTAC GCGAGATTTG AGTCCCCTCC GAAAACTCAG
    CCGACGGAGG ACAACAAGTA CAAGTTCTGC GCAAGCTGTG CACGTCTGGC CGAAATGAGG
    CAGAAGGAAA TCCCCAGGGT CGTGGAGCAG CTCCAGGACC TGGAAGGCCG CGTCCTCTAC
    AGCCTCGCCA CCAAGAACGG CGTCCAGTAC CGGGTGGGCG ATGGCGTGTA CCTCCCTCCC
    GAGGCCTTCA CCTTCAACAT CAAGCTGTCC AGTCCTGTGA AACGCCCCCG GAAGGAGCCT
    GTGGACGAAG CTCTGTATCC AGAACACTAC CGGAAGTACT CTGACTACAT CAAGGGCAGC
    AACCTGGATG CCCCTGAGCC CTACCGTATT GGCCGCATAA AGGAGATCTT CTGCAGCAAG
    AAGAGCAACG GCCGGCCCAA TGAGACAGAC ATCAAGATCA GGGTCAACAA GTTCTACAGG
    CCGGAGAACA CACACAAGTC TACCCCAGCC AGTTACCACG CAGACATCAA CCTGCTTTAC
    TGGAGCGATG AGGAGGCCGT GGTGGACTTC AAGGCCGTGC AGGGCCGCTG CACCGTGGAG
    TACGGAGAGG ACCTGCCTCA GTGCCTCCAG GACTTCTCCG CTGGTGGCCC CGATCGCTTC
    TATTTTCTCG AGGCCTATAA CGCCAAGAGC AAAAGCTTTG AAGATCCTCC GAACCACGCC
    CGGAGCACCG GAAATAAAGG GAAAGGGAAG GGGAAAGGAA AAAACAGGAC GAAATCTCAG
    ACGTGTGAGC CGAGTGAACT GGAGACAGAA ATCAAACTGC CGAAGCTGCG GACCCTGGAC
    GTGTTTTCCG GCTGTGGGGG ATTGTCGGAA GGCTTCCACC AAGCAGGCAT CTCGGAGACA
    CTTTGGGCCA TCGAGATGTG GGACCCTGCG GCCCAGGCGT TCCGGCTCAA CAACCCTGGG
    TCCACGGTGT TCACAGAGGA CTGCAACGTC CTGCTGAAGC TGGTCATGGC TGGGGAGGTG
    ACCAACTCCC GCGGCCAGAA GCTGCCTCAG AAGGGAGATG TGGAGATGCT GTGCGGCGGG
    CCGCCCTGCC AGGGCTTCAG CGGCATGAAC CGCTTCAACT CTCGAACCTA CTCCAAATTC
    AAGAACTCTC TGGTGGTCTC TTTCCTCAGC TACTGTGACT ACTACCGGCC CCGCTACTTC
    CTCTTGGAGA ACGTTCGGAA CTTCGTCTCC TTCAAGCGCT CCATGGTCCT GAAGCTGACG
    CTGCGCTGCC TGGTCCGCAT GGGCTACCAG TGCACCTTTG GCGTGCTGCA GGCTGGTCAG
    TACGGCGTGG CCCAGACTCG GAGGCGAGCC ATCATCCTGG CTGCAGCCCC TGGGGAGCCA
    CTCCCGCTGT TCCCGGAGCC GTTGCATGTG TTCGCACCCC GGGCCTGCCA GCTGAGCGTC
    GTAGTGGACG ACAAGAAGTT TGTCAGCAAC ATCACCAGGT TGAGCTCGGG TCCCTTCCGA
    ACCATCACCG TGCGGGACAC CATGTCTGAC CTCCCTGAGA TCCGGAACGG GGCCTCGGCA
    CTGGAGATTT CATACAACGG GGAGCCCCAG TCCTGGTTCC AGAGGCAGCT CCGGGGCTCG
    CAGTACCAGC CCATCCTCAG GGATCATATT TGCAAGGACA TGAGCGCCTT GGTGGCTGCC
    CGCATGCGGC ACATCCCCCT GGCCCCGGGC TCGGACTGGC GTGACCTGCC CAACATTGAG
    GTGCGGCTCT CTGACGGCAC CCTGGCCCGG AAGCTGCGGT ACAACTACCA CGACAAGAAG
    AACGGCTGCA GCAGCAGCGG CGCCCTCCGT GGGGTCTGCT CCTGTGTGGA AGGCAAGCCC
    TGTGAGCCTG CGGCCCGACA GTTTAACACC CTTATCCCCT GGTGCCTGCC CCACACTGGG
    AACAGGCACA ACCACTGGGC CGGCCTCTAC GGGCGTCTCG AGTGGGACGG CTTCTTCAGC
    ACAACTGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCCGAGCAG
    CACCGAGTGG TGAGCGTCCG GGAGTGCGCC CGCTCCCAGG GCTTCCCCGA CACCTATCGG
    CTGTTCGGCA ACATCCTAGA CAAGCACCGG CAGGTGGGTA ATGCTGTGCC GCCGCCACTG
    GCCAAAGCCA TCGGCTTGGA GATCAAGCGC TGCATGTTGG CCAAAGCGCG CGAGAGCGCC
    TCAGGGCATA TAAAAGTCAT GTTTGGGGGA TTCCTTGATG GCTTAGTGGT AAAGAATCTG
    CCTGCCAGTG CAGGAGACAC GGATTCAATC CCTGATCCAG GAAGATCCCA CATGCTGCAG
    AGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327478.1
    CDS75..5003
    Translation

    Target ORF information:

    RefSeq Version XM_015471992.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471992.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGGAGGAG
    AAGAGACGCA GAACTACATA CAGAGAACTA ACCGAGAAGA AAATGACTCG AACCAAAATA
    GCCGTAGTGT CCAAGACCAA TCCTCCGAAG TGCACCGAGT GCTTGCAGTA CCTGGACGAC
    CCTGAGCTGA GATACGAGCA GCACCCCCCC GATGCGGTGG AAGAGATACA GATACTGACC
    AACGAGAGGT TGTCCATCTT TGATGCCAAC GAATCTGGCT TTGAGAGTTA CGAGGATTTG
    CCTCAGCACA AACTAACCTG CTTCAGCGTG TACTGTAAAC GCGGTCACCT TTGCCCGATC
    GACACCGGCC TCATTGAGAA GGATGTCGAG CTCCTCTTTT CTGGTTCAGC AAAGCCGATA
    TATGAGGATG ACCCATCTCC CGAAGGTGGT ATTAATGGCA AAAATTTTGG CCCCATAAAC
    GAATGGTGGA TTGCTGGTTT TGATGGAGGT GAAAAGGCTC TTCTTGGCTT TAGCACCTCA
    TTTGCCGAGT ATATCTTGAT GGATCCCAGC CCAGAGTACG CACCACTATT CAGCGTGATG
    CAGGAGAAGA TCTATATAAG TAAGATAGTG GTTGAGTTCC TGCAGAGCAA CCCTGACTCC
    ACCTACGAAG ACCTGATCAA TAAGATTGAG ACCACCGTTC CTCCTTGTAT GCTCAACTTG
    AATCGATTCA CAGAGGATTC TCTCCTGCGG CATGCCCAGT TCGTGGTGGA GCAAGTAGAG
    AGTTATGATC GGGCTGGGGA CAGTGACGAG CAGCCCATCT TCCTGAGCCC CTGCATGAGA
    GACCTCATCA AGCTGGCCGG GGTCACCCTG GGAAAAAGGC GAGCCGAGAG GCGGCAGACC
    ATCCGGCAAC CCGCCAAAGA GAAGGACAAG GGCCCCACCA AGGCCACCAC CACCAAGCTG
    GTCTACCAGA TCTTTGACAC TTTCTTTGCG GAGCAAATTG AAAAAGATGA CAAGGAAGAC
    AAGGAGAATG CCTTCAAGCG CCGGCGCTGT GGCGTCTGTG AGATTTGTCA ACAGCCCGAG
    TGTGGAAAGT GTAAGGCCTG TAAGGATATG GTTAAATTTG GTGGTAGCGG ACGGAGCAAG
    CAGGCTTGCC AAAAGAGGAG GTGTCCCAAC ATGGCCATGA AGGAGGCAGA CGATGACGAG
    GAAGTGGATG ACAATATTCC AGAGATGCCA TCACCCAAAA AGATGCATCA GGGGAAGAAA
    AAGAAGCAGA ATAAGAATCG GATCTCTTGG GTTGGCGATG CCGTCAAGAC TGACGGGAAG
    AAGAGTTACT ACAAGAAGGT ATGCATCGAC TCGGAAACCC TGGAAGTGGG GGACTGTGTT
    TCTGTAATTC CAGACGACTC TTCAAAACCA CTGTATCTAG CAAGGGTCAC GGCGCTGTGG
    GAGGACAGCA GCAATGGGCA GATGTTCCAT GCCCACTGGT TCTGTGCTGG GACGGACACG
    GTCCTCGGGG CCACATCGGA CCCCCTGGAG CTGTTCCTGG TTGACGAGTG TGAGGACATG
    CAGCTCTCGT ACATCCACAG CAAGGTGCAG GTCATTTATA AGGCGCCCTC AGAGAACTGG
    GCCATGGAGG GAGGCGTGGA CCCCGAGGCC CTGATGTCAG AGGACGACGG GAAGACCTAC
    TTCTACCAGC TGTGGTACGA CCAAGACTAC GCGAGATTTG AGTCCCCTCC GAAAACTCAG
    CCGACGGAGG ACAACAAGTA CAAGTTCTGC GCAAGCTGTG CACGTCTGGC CGAAATGAGG
    CAGAAGGAAA TCCCCAGGGT CGTGGAGCAG CTCCAGGACC TGGAAGGCCG CGTCCTCTAC
    AGCCTCGCCA CCAAGAACGG CGTCCAGTAC CGGGTGGGCG ATGGCGTGTA CCTCCCTCCC
    GAGGCCTTCA CCTTCAACAT CAAGCTGTCC AGTCCTGTGA AACGCCCCCG GAAGGAGCCT
    GTGGACGAAG CTCTGTATCC AGAACACTAC CGGAAGTACT CTGACTACAT CAAGGGCAGC
    AACCTGGATG CCCCTGAGCC CTACCGTATT GGCCGCATAA AGGAGATCTT CTGCAGCAAG
    AAGAGCAACG GCCGGCCCAA TGAGACAGAC ATCAAGATCA GGGTCAACAA GTTCTACAGG
    CCGGAGAACA CACACAAGTC TACCCCAGCC AGTTACCACG CAGACATCAA CCTGCTTTAC
    TGGAGCGATG AGGAGGCCGT GGTGGACTTC AAGGCCGTGC AGGGCCGCTG CACCGTGGAG
    TACGGAGAGG ACCTGCCTCA GTGCCTCCAG GACTTCTCCG CTGGTGGCCC CGATCGCTTC
    TATTTTCTCG AGGCCTATAA CGCCAAGAGC AAAAGCTTTG AAGATCCTCC GAACCACGCC
    CGGAGCACCG GAAATAAAGG GAAAGGGAAG GGGAAAGGAA AAAACAGGAC GAAATCTCAG
    ACGTGTGAGC CGAGTGAACT GGAGACAGAA ATCAAACTGC CGAAGCTGCG GACCCTGGAC
    GTGTTTTCCG GCTGTGGGGG ATTGTCGGAA GGCTTCCACC AAGCAGGCAT CTCGGAGACA
    CTTTGGGCCA TCGAGATGTG GGACCCTGCG GCCCAGGCGT TCCGGCTCAA CAACCCTGGG
    TCCACGGTGT TCACAGAGGA CTGCAACGTC CTGCTGAAGC TGGTCATGGC TGGGGAGGTG
    ACCAACTCCC GCGGCCAGAA GCTGCCTCAG AAGGGAGATG TGGAGATGCT GTGCGGCGGG
    CCGCCCTGCC AGGGCTTCAG CGGCATGAAC CGCTTCAACT CTCGAACCTA CTCCAAATTC
    AAGAACTCTC TGGTGGTCTC TTTCCTCAGC TACTGTGACT ACTACCGGCC CCGCTACTTC
    CTCTTGGAGA ACGTTCGGAA CTTCGTCTCC TTCAAGCGCT CCATGGTCCT GAAGCTGACG
    CTGCGCTGCC TGGTCCGCAT GGGCTACCAG TGCACCTTTG GCGTGCTGCA GGCTGGTCAG
    TACGGCGTGG CCCAGACTCG GAGGCGAGCC ATCATCCTGG CTGCAGCCCC TGGGGAGCCA
    CTCCCGCTGT TCCCGGAGCC GTTGCATGTG TTCGCACCCC GGGCCTGCCA GCTGAGCGTC
    GTAGTGGACG ACAAGAAGTT TGTCAGCAAC ATCACCAGGT TGAGCTCGGG TCCCTTCCGA
    ACCATCACCG TGCGGGACAC CATGTCTGAC CTCCCTGAGA TCCGGAACGG GGCCTCGGCA
    CTGGAGATTT CATACAACGG GGAGCCCCAG TCCTGGTTCC AGAGGCAGCT CCGGGGCTCG
    CAGTACCAGC CCATCCTCAG GGATCATATT TGCAAGGACA TGAGCGCCTT GGTGGCTGCC
    CGCATGCGGC ACATCCCCCT GGCCCCGGGC TCGGACTGGC GTGACCTGCC CAACATTGAG
    GTGCGGCTCT CTGACGGCAC CCTGGCCCGG AAGCTGCGGT ACAACTACCA CGACAAGAAG
    AACGGCTGCA GCAGCAGCGG CGCCCTCCGT GGGGTCTGCT CCTGTGTGGA AGGCAAGCCC
    TGTGAGCCTG CGGCCCGACA GTTTAACACC CTTATCCCCT GGTGCCTGCC CCACACTGGG
    AACAGGCACA ACCACTGGGC CGGCCTCTAC GGGCGTCTCG AGTGGGACGG CTTCTTCAGC
    ACAACTGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCCGAGCAG
    CACCGAGTGG TGAGCGTCCG GGAGTGCGCC CGCTCCCAGG GCTTCCCCGA CACCTATCGG
    CTGTTCGGCA ACATCCTAGA CAAGCACCGG CAGGTGGGTA ATGCTGTGCC GCCGCCACTG
    GCCAAAGCCA TCGGCTTGGA GATCAAGCGC TGCATGTTGG CCAAAGCGCG CGAGAGCGCC
    TCAGGGCATA TAAAAGTCAT GTTTGGGGGA TTCCTTGATG GCTTAGTGGT AAAGAATCTG
    CCTGCCAGTG CAGGAGACAC GGATTCAATC CCTGATCCAG GAAGATCCCA CATGCTGCAG
    AGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91053
    Clone ID Related Accession (Same CDS sequence) XM_015471992.1 , XM_015471992.2
    Accession Version XM_015471992.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGGAGGAG
    AAGAGACGCA GAACTACATA CAGAGAACTA ACCGAGAAGA AAATGACTCG AACCAAAATA
    GCCGTAGTGT CCAAGACCAA TCCTCCGAAG TGCACCGAGT GCTTGCAGTA CCTGGACGAC
    CCTGAGCTGA GATACGAGCA GCACCCCCCC GATGCGGTGG AAGAGATACA GATACTGACC
    AACGAGAGGT TGTCCATCTT TGATGCCAAC GAATCTGGCT TTGAGAGTTA CGAGGATTTG
    CCTCAGCACA AACTAACCTG CTTCAGCGTG TACTGTAAAC GCGGTCACCT TTGCCCGATC
    GACACCGGCC TCATTGAGAA GGATGTCGAG CTCCTCTTTT CTGGTTCAGC AAAGCCGATA
    TATGAGGATG ACCCATCTCC CGAAGGTGGT ATTAATGGCA AAAATTTTGG CCCCATAAAC
    GAATGGTGGA TTGCTGGTTT TGATGGAGGT GAAAAGGCTC TTCTTGGCTT TAGCACCTCA
    TTTGCCGAGT ATATCTTGAT GGATCCCAGC CCAGAGTACG CACCACTATT CAGCGTGATG
    CAGGAGAAGA TCTATATAAG TAAGATAGTG GTTGAGTTCC TGCAGAGCAA CCCTGACTCC
    ACCTACGAAG ACCTGATCAA TAAGATTGAG ACCACCGTTC CTCCTTGTAT GCTCAACTTG
    AATCGATTCA CAGAGGATTC TCTCCTGCGG CATGCCCAGT TCGTGGTGGA GCAAGTAGAG
    AGTTATGATC GGGCTGGGGA CAGTGACGAG CAGCCCATCT TCCTGAGCCC CTGCATGAGA
    GACCTCATCA AGCTGGCCGG GGTCACCCTG GGAAAAAGGC GAGCCGAGAG GCGGCAGACC
    ATCCGGCAAC CCGCCAAAGA GAAGGACAAG GGCCCCACCA AGGCCACCAC CACCAAGCTG
    GTCTACCAGA TCTTTGACAC TTTCTTTGCG GAGCAAATTG AAAAAGATGA CAAGGAAGAC
    AAGGAGAATG CCTTCAAGCG CCGGCGCTGT GGCGTCTGTG AGATTTGTCA ACAGCCCGAG
    TGTGGAAAGT GTAAGGCCTG TAAGGATATG GTTAAATTTG GTGGTAGCGG ACGGAGCAAG
    CAGGCTTGCC AAAAGAGGAG GTGTCCCAAC ATGGCCATGA AGGAGGCAGA CGATGACGAG
    GAAGTGGATG ACAATATTCC AGAGATGCCA TCACCCAAAA AGATGCATCA GGGGAAGAAA
    AAGAAGCAGA ATAAGAATCG GATCTCTTGG GTTGGCGATG CCGTCAAGAC TGACGGGAAG
    AAGAGTTACT ACAAGAAGGT ATGCATCGAC TCGGAAACCC TGGAAGTGGG GGACTGTGTT
    TCTGTAATTC CAGACGACTC TTCAAAACCA CTGTATCTAG CAAGGGTCAC GGCGCTGTGG
    GAGGACAGCA GCAATGGGCA GATGTTCCAT GCCCACTGGT TCTGTGCTGG GACGGACACG
    GTCCTCGGGG CCACATCGGA CCCCCTGGAG CTGTTCCTGG TTGACGAGTG TGAGGACATG
    CAGCTCTCGT ACATCCACAG CAAGGTGCAG GTCATTTATA AGGCGCCCTC AGAGAACTGG
    GCCATGGAGG GAGGCGTGGA CCCCGAGGCC CTGATGTCAG AGGACGACGG GAAGACCTAC
    TTCTACCAGC TGTGGTACGA CCAAGACTAC GCGAGATTTG AGTCCCCTCC GAAAACTCAG
    CCGACGGAGG ACAACAAGTA CAAGTTCTGC GCAAGCTGTG CACGTCTGGC CGAAATGAGG
    CAGAAGGAAA TCCCCAGGGT CGTGGAGCAG CTCCAGGACC TGGAAGGCCG CGTCCTCTAC
    AGCCTCGCCA CCAAGAACGG CGTCCAGTAC CGGGTGGGCG ATGGCGTGTA CCTCCCTCCC
    GAGGCCTTCA CCTTCAACAT CAAGCTGTCC AGTCCTGTGA AACGCCCCCG GAAGGAGCCT
    GTGGACGAAG CTCTGTATCC AGAACACTAC CGGAAGTACT CTGACTACAT CAAGGGCAGC
    AACCTGGATG CCCCTGAGCC CTACCGTATT GGCCGCATAA AGGAGATCTT CTGCAGCAAG
    AAGAGCAACG GCCGGCCCAA TGAGACAGAC ATCAAGATCA GGGTCAACAA GTTCTACAGG
    CCGGAGAACA CACACAAGTC TACCCCAGCC AGTTACCACG CAGACATCAA CCTGCTTTAC
    TGGAGCGATG AGGAGGCCGT GGTGGACTTC AAGGCCGTGC AGGGCCGCTG CACCGTGGAG
    TACGGAGAGG ACCTGCCTCA GTGCCTCCAG GACTTCTCCG CTGGTGGCCC CGATCGCTTC
    TATTTTCTCG AGGCCTATAA CGCCAAGAGC AAAAGCTTTG AAGATCCTCC GAACCACGCC
    CGGAGCACCG GAAATAAAGG GAAAGGGAAG GGGAAAGGAA AAAACAGGAC GAAATCTCAG
    ACGTGTGAGC CGAGTGAACT GGAGACAGAA ATCAAACTGC CGAAGCTGCG GACCCTGGAC
    GTGTTTTCCG GCTGTGGGGG ATTGTCGGAA GGCTTCCACC AAGCAGGCAT CTCGGAGACA
    CTTTGGGCCA TCGAGATGTG GGACCCTGCG GCCCAGGCGT TCCGGCTCAA CAACCCTGGG
    TCCACGGTGT TCACAGAGGA CTGCAACGTC CTGCTGAAGC TGGTCATGGC TGGGGAGGTG
    ACCAACTCCC GCGGCCAGAA GCTGCCTCAG AAGGGAGATG TGGAGATGCT GTGCGGCGGG
    CCGCCCTGCC AGGGCTTCAG CGGCATGAAC CGCTTCAACT CTCGAACCTA CTCCAAATTC
    AAGAACTCTC TGGTGGTCTC TTTCCTCAGC TACTGTGACT ACTACCGGCC CCGCTACTTC
    CTCTTGGAGA ACGTTCGGAA CTTCGTCTCC TTCAAGCGCT CCATGGTCCT GAAGCTGACG
    CTGCGCTGCC TGGTCCGCAT GGGCTACCAG TGCACCTTTG GCGTGCTGCA GGCTGGTCAG
    TACGGCGTGG CCCAGACTCG GAGGCGAGCC ATCATCCTGG CTGCAGCCCC TGGGGAGCCA
    CTCCCGCTGT TCCCGGAGCC GTTGCATGTG TTCGCACCCC GGGCCTGCCA GCTGAGCGTC
    GTAGTGGACG ACAAGAAGTT TGTCAGCAAC ATCACCAGGT TGAGCTCGGG TCCCTTCCGA
    ACCATCACCG TGCGGGACAC CATGTCTGAC CTCCCTGAGA TCCGGAACGG GGCCTCGGCA
    CTGGAGATTT CATACAACGG GGAGCCCCAG TCCTGGTTCC AGAGGCAGCT CCGGGGCTCG
    CAGTACCAGC CCATCCTCAG GGATCATATT TGCAAGGACA TGAGCGCCTT GGTGGCTGCC
    CGCATGCGGC ACATCCCCCT GGCCCCGGGC TCGGACTGGC GTGACCTGCC CAACATTGAG
    GTGCGGCTCT CTGACGGCAC CCTGGCCCGG AAGCTGCGGT ACAACTACCA CGACAAGAAG
    AACGGCTGCA GCAGCAGCGG CGCCCTCCGT GGGGTCTGCT CCTGTGTGGA AGGCAAGCCC
    TGTGAGCCTG CGGCCCGACA GTTTAACACC CTTATCCCCT GGTGCCTGCC CCACACTGGG
    AACAGGCACA ACCACTGGGC CGGCCTCTAC GGGCGTCTCG AGTGGGACGG CTTCTTCAGC
    ACAACTGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCCGAGCAG
    CACCGAGTGG TGAGCGTCCG GGAGTGCGCC CGCTCCCAGG GCTTCCCCGA CACCTATCGG
    CTGTTCGGCA ACATCCTAGA CAAGCACCGG CAGGTGGGTA ATGCTGTGCC GCCGCCACTG
    GCCAAAGCCA TCGGCTTGGA GATCAAGCGC TGCATGTTGG CCAAAGCGCG CGAGAGCGCC
    TCAGGGCATA TAAAAGTCAT GTTTGGGGGA TTCCTTGATG GCTTAGTGGT AAAGAATCTG
    CCTGCCAGTG CAGGAGACAC GGATTCAATC CCTGATCCAG GAAGATCCCA CATGCTGCAG
    AGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327478.1
    CDS79..5007
    Translation

    Target ORF information:

    RefSeq Version XM_015471992.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471992.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGGAGGAG
    AAGAGACGCA GAACTACATA CAGAGAACTA ACCGAGAAGA AAATGACTCG AACCAAAATA
    GCCGTAGTGT CCAAGACCAA TCCTCCGAAG TGCACCGAGT GCTTGCAGTA CCTGGACGAC
    CCTGAGCTGA GATACGAGCA GCACCCCCCC GATGCGGTGG AAGAGATACA GATACTGACC
    AACGAGAGGT TGTCCATCTT TGATGCCAAC GAATCTGGCT TTGAGAGTTA CGAGGATTTG
    CCTCAGCACA AACTAACCTG CTTCAGCGTG TACTGTAAAC GCGGTCACCT TTGCCCGATC
    GACACCGGCC TCATTGAGAA GGATGTCGAG CTCCTCTTTT CTGGTTCAGC AAAGCCGATA
    TATGAGGATG ACCCATCTCC CGAAGGTGGT ATTAATGGCA AAAATTTTGG CCCCATAAAC
    GAATGGTGGA TTGCTGGTTT TGATGGAGGT GAAAAGGCTC TTCTTGGCTT TAGCACCTCA
    TTTGCCGAGT ATATCTTGAT GGATCCCAGC CCAGAGTACG CACCACTATT CAGCGTGATG
    CAGGAGAAGA TCTATATAAG TAAGATAGTG GTTGAGTTCC TGCAGAGCAA CCCTGACTCC
    ACCTACGAAG ACCTGATCAA TAAGATTGAG ACCACCGTTC CTCCTTGTAT GCTCAACTTG
    AATCGATTCA CAGAGGATTC TCTCCTGCGG CATGCCCAGT TCGTGGTGGA GCAAGTAGAG
    AGTTATGATC GGGCTGGGGA CAGTGACGAG CAGCCCATCT TCCTGAGCCC CTGCATGAGA
    GACCTCATCA AGCTGGCCGG GGTCACCCTG GGAAAAAGGC GAGCCGAGAG GCGGCAGACC
    ATCCGGCAAC CCGCCAAAGA GAAGGACAAG GGCCCCACCA AGGCCACCAC CACCAAGCTG
    GTCTACCAGA TCTTTGACAC TTTCTTTGCG GAGCAAATTG AAAAAGATGA CAAGGAAGAC
    AAGGAGAATG CCTTCAAGCG CCGGCGCTGT GGCGTCTGTG AGATTTGTCA ACAGCCCGAG
    TGTGGAAAGT GTAAGGCCTG TAAGGATATG GTTAAATTTG GTGGTAGCGG ACGGAGCAAG
    CAGGCTTGCC AAAAGAGGAG GTGTCCCAAC ATGGCCATGA AGGAGGCAGA CGATGACGAG
    GAAGTGGATG ACAATATTCC AGAGATGCCA TCACCCAAAA AGATGCATCA GGGGAAGAAA
    AAGAAGCAGA ATAAGAATCG GATCTCTTGG GTTGGCGATG CCGTCAAGAC TGACGGGAAG
    AAGAGTTACT ACAAGAAGGT ATGCATCGAC TCGGAAACCC TGGAAGTGGG GGACTGTGTT
    TCTGTAATTC CAGACGACTC TTCAAAACCA CTGTATCTAG CAAGGGTCAC GGCGCTGTGG
    GAGGACAGCA GCAATGGGCA GATGTTCCAT GCCCACTGGT TCTGTGCTGG GACGGACACG
    GTCCTCGGGG CCACATCGGA CCCCCTGGAG CTGTTCCTGG TTGACGAGTG TGAGGACATG
    CAGCTCTCGT ACATCCACAG CAAGGTGCAG GTCATTTATA AGGCGCCCTC AGAGAACTGG
    GCCATGGAGG GAGGCGTGGA CCCCGAGGCC CTGATGTCAG AGGACGACGG GAAGACCTAC
    TTCTACCAGC TGTGGTACGA CCAAGACTAC GCGAGATTTG AGTCCCCTCC GAAAACTCAG
    CCGACGGAGG ACAACAAGTA CAAGTTCTGC GCAAGCTGTG CACGTCTGGC CGAAATGAGG
    CAGAAGGAAA TCCCCAGGGT CGTGGAGCAG CTCCAGGACC TGGAAGGCCG CGTCCTCTAC
    AGCCTCGCCA CCAAGAACGG CGTCCAGTAC CGGGTGGGCG ATGGCGTGTA CCTCCCTCCC
    GAGGCCTTCA CCTTCAACAT CAAGCTGTCC AGTCCTGTGA AACGCCCCCG GAAGGAGCCT
    GTGGACGAAG CTCTGTATCC AGAACACTAC CGGAAGTACT CTGACTACAT CAAGGGCAGC
    AACCTGGATG CCCCTGAGCC CTACCGTATT GGCCGCATAA AGGAGATCTT CTGCAGCAAG
    AAGAGCAACG GCCGGCCCAA TGAGACAGAC ATCAAGATCA GGGTCAACAA GTTCTACAGG
    CCGGAGAACA CACACAAGTC TACCCCAGCC AGTTACCACG CAGACATCAA CCTGCTTTAC
    TGGAGCGATG AGGAGGCCGT GGTGGACTTC AAGGCCGTGC AGGGCCGCTG CACCGTGGAG
    TACGGAGAGG ACCTGCCTCA GTGCCTCCAG GACTTCTCCG CTGGTGGCCC CGATCGCTTC
    TATTTTCTCG AGGCCTATAA CGCCAAGAGC AAAAGCTTTG AAGATCCTCC GAACCACGCC
    CGGAGCACCG GAAATAAAGG GAAAGGGAAG GGGAAAGGAA AAAACAGGAC GAAATCTCAG
    ACGTGTGAGC CGAGTGAACT GGAGACAGAA ATCAAACTGC CGAAGCTGCG GACCCTGGAC
    GTGTTTTCCG GCTGTGGGGG ATTGTCGGAA GGCTTCCACC AAGCAGGCAT CTCGGAGACA
    CTTTGGGCCA TCGAGATGTG GGACCCTGCG GCCCAGGCGT TCCGGCTCAA CAACCCTGGG
    TCCACGGTGT TCACAGAGGA CTGCAACGTC CTGCTGAAGC TGGTCATGGC TGGGGAGGTG
    ACCAACTCCC GCGGCCAGAA GCTGCCTCAG AAGGGAGATG TGGAGATGCT GTGCGGCGGG
    CCGCCCTGCC AGGGCTTCAG CGGCATGAAC CGCTTCAACT CTCGAACCTA CTCCAAATTC
    AAGAACTCTC TGGTGGTCTC TTTCCTCAGC TACTGTGACT ACTACCGGCC CCGCTACTTC
    CTCTTGGAGA ACGTTCGGAA CTTCGTCTCC TTCAAGCGCT CCATGGTCCT GAAGCTGACG
    CTGCGCTGCC TGGTCCGCAT GGGCTACCAG TGCACCTTTG GCGTGCTGCA GGCTGGTCAG
    TACGGCGTGG CCCAGACTCG GAGGCGAGCC ATCATCCTGG CTGCAGCCCC TGGGGAGCCA
    CTCCCGCTGT TCCCGGAGCC GTTGCATGTG TTCGCACCCC GGGCCTGCCA GCTGAGCGTC
    GTAGTGGACG ACAAGAAGTT TGTCAGCAAC ATCACCAGGT TGAGCTCGGG TCCCTTCCGA
    ACCATCACCG TGCGGGACAC CATGTCTGAC CTCCCTGAGA TCCGGAACGG GGCCTCGGCA
    CTGGAGATTT CATACAACGG GGAGCCCCAG TCCTGGTTCC AGAGGCAGCT CCGGGGCTCG
    CAGTACCAGC CCATCCTCAG GGATCATATT TGCAAGGACA TGAGCGCCTT GGTGGCTGCC
    CGCATGCGGC ACATCCCCCT GGCCCCGGGC TCGGACTGGC GTGACCTGCC CAACATTGAG
    GTGCGGCTCT CTGACGGCAC CCTGGCCCGG AAGCTGCGGT ACAACTACCA CGACAAGAAG
    AACGGCTGCA GCAGCAGCGG CGCCCTCCGT GGGGTCTGCT CCTGTGTGGA AGGCAAGCCC
    TGTGAGCCTG CGGCCCGACA GTTTAACACC CTTATCCCCT GGTGCCTGCC CCACACTGGG
    AACAGGCACA ACCACTGGGC CGGCCTCTAC GGGCGTCTCG AGTGGGACGG CTTCTTCAGC
    ACAACTGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCCGAGCAG
    CACCGAGTGG TGAGCGTCCG GGAGTGCGCC CGCTCCCAGG GCTTCCCCGA CACCTATCGG
    CTGTTCGGCA ACATCCTAGA CAAGCACCGG CAGGTGGGTA ATGCTGTGCC GCCGCCACTG
    GCCAAAGCCA TCGGCTTGGA GATCAAGCGC TGCATGTTGG CCAAAGCGCG CGAGAGCGCC
    TCAGGGCATA TAAAAGTCAT GTTTGGGGGA TTCCTTGATG GCTTAGTGGT AAAGAATCTG
    CCTGCCAGTG CAGGAGACAC GGATTCAATC CCTGATCCAG GAAGATCCCA CATGCTGCAG
    AGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91054
    Clone ID Related Accession (Same CDS sequence) XM_015471993.1
    Accession Version XM_015471993.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATTTTCT TTGCAGCTCA GGGGCAGATG TGGTGTGTTT TCACTTCGTG CACCAGAAGG 
    CTCAAAGATT TGGAAAGAGA TAGTTTGACA GAAAAGGAAT GTGTGAAGGA GAAACTGAAT
    CTCTTGCACG AATTTCTGCG GACAGAAATA AAGAATCAGT TATGTGATTT GGAAACCAAA
    TTGCATAAAG AAGAATTATC TGAGGAGGGC TACCTGGCTA AAGTCAAATC CCTTTTAAAT
    AAAGATTTGT CCTTGGAGAA CGGAGCTCAT GCTTTCAGTC GGGAAGCGAA TGGATGTCTA
    GAGAACGGGA GCCAGACAAG TGGTGAGGAT TGCAGAGTGG TAATGGCAGA GAAAGGCAAG
    CCCCCCAAAC CTGTCTCCAG ACTTTACACG CCCAGGAGAA GCAAGTCTGA TGGAGAAACA
    AAGTCTGAAG TCTCTTCTAG CCCCAGGATT ACAAGGAAGA CTACCAGGCA GACCACCATC
    ACATCTCATT TCCCACGGGG CCCTGCCAAA CGAAAACCTG AGGAAGAACC TGAAAAAGTG
    AAGTCAGACG ATTCTGTTGA TGAAGAAAAA GACCAGGAGG AAAAGAGACG TCGAGTTACA
    TCCAGAGAAC GAGTTGCTGG GCTGCTCCCT GCAGAAGAAC CAGGAAGAGT AAGACCAGGA
    ACACACATGG AAGAAGAAGG AAGAGATGAT AAAGAAGAAA AGAGACTCAG AAGTCAAACC
    AAAGAACCGA CACCTAAACA CAAAGCTAAG GAGGAGCCAG ACAGAGATGT GAGGCCTGGA
    GGAGCTCAGG CTGAAATGAA TGAAGGAGAA GACAAAGATG AAAAGAGGCA CAGAAGTCAA
    CCCAAAGATC TAGCTAGCAA ACGGAGACCA GAAGAAAAAG AACCTGAAAG AGTAAAGCCA
    CAAGTTTCTG ATGAGAAAGA TGAAGATGAA AAGTTCTGGA GAATACAGTT CACATATCAA
    AGTACCAGCA GGGAGGAGAA GAGACGCAGA ACTACATACA GAGAACTAAC CGAGAAGAAA
    ATGACTCGAA CCAAAATAGC CGTAGTGTCC AAGACCAATC CTCCGAAGTG CACCGAGTGC
    TTGCAGTACC TGGACGACCC TGAGCTGAGA TACGAGCAGC ACCCCCCCGA TGCGGTGGAA
    GAGATACAGA TACTGACCAA CGAGAGGTTG TCCATCTTTG ATGCCAACGA ATCTGGCTTT
    GAGAGTTACG AGGATTTGCC TCAGCACAAA CTAACCTGCT TCAGCGTGTA CTGTAAACGC
    GGTCACCTTT GCCCGATCGA CACCGGCCTC ATTGAGAAGG ATGTCGAGCT CCTCTTTTCT
    GGTTCAGCAA AGCCGATATA TGAGGATGAC CCATCTCCCG AAGGTGGTAT TAATGGCAAA
    AATTTTGGCC CCATAAACGA ATGGTGGATT GCTGGTTTTG ATGGAGGTGA AAAGGCTCTT
    CTTGGCTTTA GCACCTCATT TGCCGAGTAT ATCTTGATGG ATCCCAGCCC AGAGTACGCA
    CCACTATTCA GCGTGATGCA GGAGAAGATC TATATAAGTA AGATAGTGGT TGAGTTCCTG
    CAGAGCAACC CTGACTCCAC CTACGAAGAC CTGATCAATA AGATTGAGAC CACCGTTCCT
    CCTTGTATGC TCAACTTGAA TCGATTCACA GAGGATTCTC TCCTGCGGCA TGCCCAGTTC
    GTGGTGGAGC AAGTAGAGAG TTATGATCGG GCTGGGGACA GTGACGAGCA GCCCATCTTC
    CTGAGCCCCT GCATGAGAGA CCTCATCAAG CTGGCCGGGG TCACCCTGGG AAAAAGGCGA
    GCCGAGAGGC GGCAGACCAT CCGGCAACCC GCCAAAGAGA AGGACAAGGG CCCCACCAAG
    GCCACCACCA CCAAGCTGGT CTACCAGATC TTTGACACTT TCTTTGCGGA GCAAATTGAA
    AAAGATGACA AGGAAGACAA GGAGAATGCC TTCAAGCGCC GGCGCTGTGG CGTCTGTGAG
    ATTTGTCAAC AGCCCGAGTG TGGAAAGTGT AAGGCCTGTA AGGATATGGT TAAATTTGGT
    GGTAGCGGAC GGAGCAAGCA GGCTTGCCAA AAGAGGAGGT GTCCCAACAT GGCCATGAAG
    GAGGCAGACG ATGACGAGGA AGTGGATGAC AATATTCCAG AGATGCCATC ACCCAAAAAG
    ATGCATCAGG GGAAGAAAAA GAAGCAGAAT AAGAATCGGA TCTCTTGGGT TGGCGATGCC
    GTCAAGACTG ACGGGAAGAA GAGTTACTAC AAGAAGGTAT GCATCGACTC GGAAACCCTG
    GAAGTGGGGG ACTGTGTTTC TGTAATTCCA GACGACTCTT CAAAACCACT GTATCTAGCA
    AGGGTCACGG CGCTGTGGGA GGACAGCAGC AATGGGCAGA TGTTCCATGC CCACTGGTTC
    TGTGCTGGGA CGGACACGGT CCTCGGGGCC ACATCGGACC CCCTGGAGCT GTTCCTGGTT
    GACGAGTGTG AGGACATGCA GCTCTCGTAC ATCCACAGCA AGGTGCAGGT CATTTATAAG
    GCGCCCTCAG AGAACTGGGC CATGGAGGGA GGCGTGGACC CCGAGGCCCT GATGTCAGAG
    GACGACGGGA AGACCTACTT CTACCAGCTG TGGTACGACC AAGACTACGC GAGATTTGAG
    TCCCCTCCGA AAACTCAGCC GACGGAGGAC AACAAGTACA AGTTCTGCGC AAGCTGTGCA
    CGTCTGGCCG AAATGAGGCA GAAGGAAATC CCCAGGGTCG TGGAGCAGCT CCAGGACCTG
    GAAGGCCGCG TCCTCTACAG CCTCGCCACC AAGAACGGCG TCCAGTACCG GGTGGGCGAT
    GGCGTGTACC TCCCTCCCGA GGCCTTCACC TTCAACATCA AGCTGTCCAG TCCTGTGAAA
    CGCCCCCGGA AGGAGCCTGT GGACGAAGCT CTGTATCCAG AACACTACCG GAAGTACTCT
    GACTACATCA AGGGCAGCAA CCTGGATGCC CCTGAGCCCT ACCGTATTGG CCGCATAAAG
    GAGATCTTCT GCAGCAAGAA GAGCAACGGC CGGCCCAATG AGACAGACAT CAAGATCAGG
    GTCAACAAGT TCTACAGGCC GGAGAACACA CACAAGTCTA CCCCAGCCAG TTACCACGCA
    GACATCAACC TGCTTTACTG GAGCGATGAG GAGGCCGTGG TGGACTTCAA GGCCGTGCAG
    GGCCGCTGCA CCGTGGAGTA CGGAGAGGAC CTGCCTCAGT GCCTCCAGGA CTTCTCCGCT
    GGTGGCCCCG ATCGCTTCTA TTTTCTCGAG GCCTATAACG CCAAGAGCAA AAGCTTTGAA
    GATCCTCCGA ACCACGCCCG GAGCACCGGA AATAAAGGGA AAGGGAAGGG GAAAGGAAAA
    AACAGGACGA AATCTCAGAC GTGTGAGCCG AGTGAACTGG AGACAGAAAT CAAACTGCCG
    AAGCTGCGGA CCCTGGACGT GTTTTCCGGC TGTGGGGGAT TGTCGGAAGG CTTCCACCAA
    GCAGGCATCT CGGAGACACT TTGGGCCATC GAGATGTGGG ACCCTGCGGC CCAGGCGTTC
    CGGCTCAACA ACCCTGGGTC CACGGTGTTC ACAGAGGACT GCAACGTCCT GCTGAAGCTG
    GTCATGGCTG GGGAGGTGAC CAACTCCCGC GGCCAGAAGC TGCCTCAGAA GGGAGATGTG
    GAGATGCTGT GCGGCGGGCC GCCCTGCCAG GGCTTCAGCG GCATGAACCG CTTCAACTCT
    CGAACCTACT CCAAATTCAA GAACTCTCTG GTGGTCTCTT TCCTCAGCTA CTGTGACTAC
    TACCGGCCCC GCTACTTCCT CTTGGAGAAC GTTCGGAACT TCGTCTCCTT CAAGCGCTCC
    ATGGTCCTGA AGCTGACGCT GCGCTGCCTG GTCCGCATGG GCTACCAGTG CACCTTTGGC
    GTGCTGCAGG CTGGTCAGTA CGGCGTGGCC CAGACTCGGA GGCGAGCCAT CATCCTGGCT
    GCAGCCCCTG GGGAGCCACT CCCGCTGTTC CCGGAGCCGT TGCATGTGTT CGCACCCCGG
    GCCTGCCAGC TGAGCGTCGT AGTGGACGAC AAGAAGTTTG TCAGCAACAT CACCAGGTTG
    AGCTCGGGTC CCTTCCGAAC CATCACCGTG CGGGACACCA TGTCTGACCT CCCTGAGATC
    CGGAACGGGG CCTCGGCACT GGAGATTTCA TACAACGGGG AGCCCCAGTC CTGGTTCCAG
    AGGCAGCTCC GGGGCTCGCA GTACCAGCCC ATCCTCAGGG ATCATATTTG CAAGGACATG
    AGCGCCTTGG TGGCTGCCCG CATGCGGCAC ATCCCCCTGG CCCCGGGCTC GGACTGGCGT
    GACCTGCCCA ACATTGAGGT GCGGCTCTCT GACGGCACCC TGGCCCGGAA GCTGCGGTAC
    AACTACCACG ACAAGAAGAA CGGCTGCAGC AGCAGCGGCG CCCTCCGTGG GGTCTGCTCC
    TGTGTGGAAG GCAAGCCCTG TGAGCCTGCG GCCCGACAGT TTAACACCCT TATCCCCTGG
    TGCCTGCCCC ACACTGGGAA CAGGCACAAC CACTGGGCCG GCCTCTACGG GCGTCTCGAG
    TGGGACGGCT TCTTCAGCAC AACTGTCACC AACCCCGAGC CCATGGGCAA GCAGGGCCGC
    GTGCTCCACC CCGAGCAGCA CCGAGTGGTG AGCGTCCGGG AGTGCGCCCG CTCCCAGGGC
    TTCCCCGACA CCTATCGGCT GTTCGGCAAC ATCCTAGACA AGCACCGGCA GGTGGGTAAT
    GCTGTGCCGC CGCCACTGGC CAAAGCCATC GGCTTGGAGA TCAAGCGCTG CATGTTGGCC
    AAAGCGCGCG AGAGCGCCTC AGGGCATATA AAAGTCATGT TTGGGGGATT CCTTGATGGC
    TTAGTGGTAA AGAATCTGCC TGCCAGTGCA GGAGACACGG ATTCAATCCC TGATCCAGGA
    AGATCCCACA TGCTGCAGAG CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327479.1
    CDS32..4978
    Translation

    Target ORF information:

    RefSeq Version XM_015471993.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471993.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATTTTCT TTGCAGCTCA GGGGCAGATG TGGTGTGTTT TCACTTCGTG CACCAGAAGG 
    CTCAAAGATT TGGAAAGAGA TAGTTTGACA GAAAAGGAAT GTGTGAAGGA GAAACTGAAT
    CTCTTGCACG AATTTCTGCG GACAGAAATA AAGAATCAGT TATGTGATTT GGAAACCAAA
    TTGCATAAAG AAGAATTATC TGAGGAGGGC TACCTGGCTA AAGTCAAATC CCTTTTAAAT
    AAAGATTTGT CCTTGGAGAA CGGAGCTCAT GCTTTCAGTC GGGAAGCGAA TGGATGTCTA
    GAGAACGGGA GCCAGACAAG TGGTGAGGAT TGCAGAGTGG TAATGGCAGA GAAAGGCAAG
    CCCCCCAAAC CTGTCTCCAG ACTTTACACG CCCAGGAGAA GCAAGTCTGA TGGAGAAACA
    AAGTCTGAAG TCTCTTCTAG CCCCAGGATT ACAAGGAAGA CTACCAGGCA GACCACCATC
    ACATCTCATT TCCCACGGGG CCCTGCCAAA CGAAAACCTG AGGAAGAACC TGAAAAAGTG
    AAGTCAGACG ATTCTGTTGA TGAAGAAAAA GACCAGGAGG AAAAGAGACG TCGAGTTACA
    TCCAGAGAAC GAGTTGCTGG GCTGCTCCCT GCAGAAGAAC CAGGAAGAGT AAGACCAGGA
    ACACACATGG AAGAAGAAGG AAGAGATGAT AAAGAAGAAA AGAGACTCAG AAGTCAAACC
    AAAGAACCGA CACCTAAACA CAAAGCTAAG GAGGAGCCAG ACAGAGATGT GAGGCCTGGA
    GGAGCTCAGG CTGAAATGAA TGAAGGAGAA GACAAAGATG AAAAGAGGCA CAGAAGTCAA
    CCCAAAGATC TAGCTAGCAA ACGGAGACCA GAAGAAAAAG AACCTGAAAG AGTAAAGCCA
    CAAGTTTCTG ATGAGAAAGA TGAAGATGAA AAGTTCTGGA GAATACAGTT CACATATCAA
    AGTACCAGCA GGGAGGAGAA GAGACGCAGA ACTACATACA GAGAACTAAC CGAGAAGAAA
    ATGACTCGAA CCAAAATAGC CGTAGTGTCC AAGACCAATC CTCCGAAGTG CACCGAGTGC
    TTGCAGTACC TGGACGACCC TGAGCTGAGA TACGAGCAGC ACCCCCCCGA TGCGGTGGAA
    GAGATACAGA TACTGACCAA CGAGAGGTTG TCCATCTTTG ATGCCAACGA ATCTGGCTTT
    GAGAGTTACG AGGATTTGCC TCAGCACAAA CTAACCTGCT TCAGCGTGTA CTGTAAACGC
    GGTCACCTTT GCCCGATCGA CACCGGCCTC ATTGAGAAGG ATGTCGAGCT CCTCTTTTCT
    GGTTCAGCAA AGCCGATATA TGAGGATGAC CCATCTCCCG AAGGTGGTAT TAATGGCAAA
    AATTTTGGCC CCATAAACGA ATGGTGGATT GCTGGTTTTG ATGGAGGTGA AAAGGCTCTT
    CTTGGCTTTA GCACCTCATT TGCCGAGTAT ATCTTGATGG ATCCCAGCCC AGAGTACGCA
    CCACTATTCA GCGTGATGCA GGAGAAGATC TATATAAGTA AGATAGTGGT TGAGTTCCTG
    CAGAGCAACC CTGACTCCAC CTACGAAGAC CTGATCAATA AGATTGAGAC CACCGTTCCT
    CCTTGTATGC TCAACTTGAA TCGATTCACA GAGGATTCTC TCCTGCGGCA TGCCCAGTTC
    GTGGTGGAGC AAGTAGAGAG TTATGATCGG GCTGGGGACA GTGACGAGCA GCCCATCTTC
    CTGAGCCCCT GCATGAGAGA CCTCATCAAG CTGGCCGGGG TCACCCTGGG AAAAAGGCGA
    GCCGAGAGGC GGCAGACCAT CCGGCAACCC GCCAAAGAGA AGGACAAGGG CCCCACCAAG
    GCCACCACCA CCAAGCTGGT CTACCAGATC TTTGACACTT TCTTTGCGGA GCAAATTGAA
    AAAGATGACA AGGAAGACAA GGAGAATGCC TTCAAGCGCC GGCGCTGTGG CGTCTGTGAG
    ATTTGTCAAC AGCCCGAGTG TGGAAAGTGT AAGGCCTGTA AGGATATGGT TAAATTTGGT
    GGTAGCGGAC GGAGCAAGCA GGCTTGCCAA AAGAGGAGGT GTCCCAACAT GGCCATGAAG
    GAGGCAGACG ATGACGAGGA AGTGGATGAC AATATTCCAG AGATGCCATC ACCCAAAAAG
    ATGCATCAGG GGAAGAAAAA GAAGCAGAAT AAGAATCGGA TCTCTTGGGT TGGCGATGCC
    GTCAAGACTG ACGGGAAGAA GAGTTACTAC AAGAAGGTAT GCATCGACTC GGAAACCCTG
    GAAGTGGGGG ACTGTGTTTC TGTAATTCCA GACGACTCTT CAAAACCACT GTATCTAGCA
    AGGGTCACGG CGCTGTGGGA GGACAGCAGC AATGGGCAGA TGTTCCATGC CCACTGGTTC
    TGTGCTGGGA CGGACACGGT CCTCGGGGCC ACATCGGACC CCCTGGAGCT GTTCCTGGTT
    GACGAGTGTG AGGACATGCA GCTCTCGTAC ATCCACAGCA AGGTGCAGGT CATTTATAAG
    GCGCCCTCAG AGAACTGGGC CATGGAGGGA GGCGTGGACC CCGAGGCCCT GATGTCAGAG
    GACGACGGGA AGACCTACTT CTACCAGCTG TGGTACGACC AAGACTACGC GAGATTTGAG
    TCCCCTCCGA AAACTCAGCC GACGGAGGAC AACAAGTACA AGTTCTGCGC AAGCTGTGCA
    CGTCTGGCCG AAATGAGGCA GAAGGAAATC CCCAGGGTCG TGGAGCAGCT CCAGGACCTG
    GAAGGCCGCG TCCTCTACAG CCTCGCCACC AAGAACGGCG TCCAGTACCG GGTGGGCGAT
    GGCGTGTACC TCCCTCCCGA GGCCTTCACC TTCAACATCA AGCTGTCCAG TCCTGTGAAA
    CGCCCCCGGA AGGAGCCTGT GGACGAAGCT CTGTATCCAG AACACTACCG GAAGTACTCT
    GACTACATCA AGGGCAGCAA CCTGGATGCC CCTGAGCCCT ACCGTATTGG CCGCATAAAG
    GAGATCTTCT GCAGCAAGAA GAGCAACGGC CGGCCCAATG AGACAGACAT CAAGATCAGG
    GTCAACAAGT TCTACAGGCC GGAGAACACA CACAAGTCTA CCCCAGCCAG TTACCACGCA
    GACATCAACC TGCTTTACTG GAGCGATGAG GAGGCCGTGG TGGACTTCAA GGCCGTGCAG
    GGCCGCTGCA CCGTGGAGTA CGGAGAGGAC CTGCCTCAGT GCCTCCAGGA CTTCTCCGCT
    GGTGGCCCCG ATCGCTTCTA TTTTCTCGAG GCCTATAACG CCAAGAGCAA AAGCTTTGAA
    GATCCTCCGA ACCACGCCCG GAGCACCGGA AATAAAGGGA AAGGGAAGGG GAAAGGAAAA
    AACAGGACGA AATCTCAGAC GTGTGAGCCG AGTGAACTGG AGACAGAAAT CAAACTGCCG
    AAGCTGCGGA CCCTGGACGT GTTTTCCGGC TGTGGGGGAT TGTCGGAAGG CTTCCACCAA
    GCAGGCATCT CGGAGACACT TTGGGCCATC GAGATGTGGG ACCCTGCGGC CCAGGCGTTC
    CGGCTCAACA ACCCTGGGTC CACGGTGTTC ACAGAGGACT GCAACGTCCT GCTGAAGCTG
    GTCATGGCTG GGGAGGTGAC CAACTCCCGC GGCCAGAAGC TGCCTCAGAA GGGAGATGTG
    GAGATGCTGT GCGGCGGGCC GCCCTGCCAG GGCTTCAGCG GCATGAACCG CTTCAACTCT
    CGAACCTACT CCAAATTCAA GAACTCTCTG GTGGTCTCTT TCCTCAGCTA CTGTGACTAC
    TACCGGCCCC GCTACTTCCT CTTGGAGAAC GTTCGGAACT TCGTCTCCTT CAAGCGCTCC
    ATGGTCCTGA AGCTGACGCT GCGCTGCCTG GTCCGCATGG GCTACCAGTG CACCTTTGGC
    GTGCTGCAGG CTGGTCAGTA CGGCGTGGCC CAGACTCGGA GGCGAGCCAT CATCCTGGCT
    GCAGCCCCTG GGGAGCCACT CCCGCTGTTC CCGGAGCCGT TGCATGTGTT CGCACCCCGG
    GCCTGCCAGC TGAGCGTCGT AGTGGACGAC AAGAAGTTTG TCAGCAACAT CACCAGGTTG
    AGCTCGGGTC CCTTCCGAAC CATCACCGTG CGGGACACCA TGTCTGACCT CCCTGAGATC
    CGGAACGGGG CCTCGGCACT GGAGATTTCA TACAACGGGG AGCCCCAGTC CTGGTTCCAG
    AGGCAGCTCC GGGGCTCGCA GTACCAGCCC ATCCTCAGGG ATCATATTTG CAAGGACATG
    AGCGCCTTGG TGGCTGCCCG CATGCGGCAC ATCCCCCTGG CCCCGGGCTC GGACTGGCGT
    GACCTGCCCA ACATTGAGGT GCGGCTCTCT GACGGCACCC TGGCCCGGAA GCTGCGGTAC
    AACTACCACG ACAAGAAGAA CGGCTGCAGC AGCAGCGGCG CCCTCCGTGG GGTCTGCTCC
    TGTGTGGAAG GCAAGCCCTG TGAGCCTGCG GCCCGACAGT TTAACACCCT TATCCCCTGG
    TGCCTGCCCC ACACTGGGAA CAGGCACAAC CACTGGGCCG GCCTCTACGG GCGTCTCGAG
    TGGGACGGCT TCTTCAGCAC AACTGTCACC AACCCCGAGC CCATGGGCAA GCAGGGCCGC
    GTGCTCCACC CCGAGCAGCA CCGAGTGGTG AGCGTCCGGG AGTGCGCCCG CTCCCAGGGC
    TTCCCCGACA CCTATCGGCT GTTCGGCAAC ATCCTAGACA AGCACCGGCA GGTGGGTAAT
    GCTGTGCCGC CGCCACTGGC CAAAGCCATC GGCTTGGAGA TCAAGCGCTG CATGTTGGCC
    AAAGCGCGCG AGAGCGCCTC AGGGCATATA AAAGTCATGT TTGGGGGATT CCTTGATGGC
    TTAGTGGTAA AGAATCTGCC TGCCAGTGCA GGAGACACGG ATTCAATCCC TGATCCAGGA
    AGATCCCACA TGCTGCAGAG CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91055
    Clone ID Related Accession (Same CDS sequence) XM_015471995.1 , XM_015471995.2
    Accession Version XM_015471995.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCAGCCT CCAGAGACAG ATGCCGCCGC CGGTCGGGGC CCTGGCGCGG CCGGCTCAGG 
    CTCAAAGATT TGGAAAGAGA TAGTTTGACA GAAAAGGAAT GTGTGAAGGA GAAACTGAAT
    CTCTTGCACG AATTTCTGCG GACAGAAATA AAGAATCAGT TATGTGATTT GGAAACCAAA
    TTGCATAAAG AAGAATTATC TGAGGAGGGC TACCTGGCTA AAGTCAAATC CCTTTTAAAT
    AAAGATTTGT CCTTGGAGAA CGGAGCTCAT GCTTTCAGTC GGGAAGCGAA TGGATGTCTA
    GAGAACGGGA GCCAGACAAG TGGTGAGGAT TGCAGAGTGG TAATGGCAGA GAAAGGCAAG
    CCCCCCAAAC CTGTCTCCAG ACTTTACACG CCCAGGAGAA GCAAGTCTGA TGGAGAAACA
    AAGTCTGAAG TCTCTTCTAG CCCCAGGATT ACAAGGAAGA CTACCAGGCA GACCACCATC
    ACATCTCATT TCCCACGGGG CCCTGCCAAA CGAAAACCTG AGGAAGAACC TGAAAAAGTG
    AAGTCAGACG ATTCTGTTGA TGAAGAAAAA GACCAGGAGG AAAAGAGACG TCGAGTTACA
    TCCAGAGAAC GAGTTGCTGG GCTGCTCCCT GCAGAAGAAC CAGGAAGAGT AAGACCAGGA
    ACACACATGG AAGAAGAAGG AAGAGATGAT AAAGAAGAAA AGAGACTCAG AAGTCAAACC
    AAAGAACCGA CACCTAAACA CAAAGCTAAG GAGGAGCCAG ACAGAGATGT GAGGCCTGGA
    GGAGCTCAGG CTGAAATGAA TGAAGGAGAA GACAAAGATG AAAAGAGGCA CAGAAGTCAA
    CCCAAAGATC TAGCTAGCAA ACGGAGACCA GAAGAAAAAG AACCTGAAAG AGTAAAGCCA
    CAAGTTTCTG ATGAGAAAGA TGAAGATGAA AAGTTCTGGA GAATACAGTT CACATATCAA
    AGTACCAGCA GGGAGGAGAA GAGACGCAGA ACTACATACA GAGAACTAAC CGAGAAGAAA
    ATGACTCGAA CCAAAATAGC CGTAGTGTCC AAGACCAATC CTCCGAAGTG CACCGAGTGC
    TTGCAGTACC TGGACGACCC TGAGCTGAGA TACGAGCAGC ACCCCCCCGA TGCGGTGGAA
    GAGATACAGA TACTGACCAA CGAGAGGTTG TCCATCTTTG ATGCCAACGA ATCTGGCTTT
    GAGAGTTACG AGGATTTGCC TCAGCACAAA CTAACCTGCT TCAGCGTGTA CTGTAAACGC
    GGTCACCTTT GCCCGATCGA CACCGGCCTC ATTGAGAAGG ATGTCGAGCT CCTCTTTTCT
    GGTTCAGCAA AGCCGATATA TGAGGATGAC CCATCTCCCG AAGGTGGTAT TAATGGCAAA
    AATTTTGGCC CCATAAACGA ATGGTGGATT GCTGGTTTTG ATGGAGGTGA AAAGGCTCTT
    CTTGGCTTTA GCACCTCATT TGCCGAGTAT ATCTTGATGG ATCCCAGCCC AGAGTACGCA
    CCACTATTCA GCGTGATGCA GGAGAAGATC TATATAAGTA AGATAGTGGT TGAGTTCCTG
    CAGAGCAACC CTGACTCCAC CTACGAAGAC CTGATCAATA AGATTGAGAC CACCGTTCCT
    CCTTGTATGC TCAACTTGAA TCGATTCACA GAGGATTCTC TCCTGCGGCA TGCCCAGTTC
    GTGGTGGAGC AAGTAGAGAG TTATGATCGG GCTGGGGACA GTGACGAGCA GCCCATCTTC
    CTGAGCCCCT GCATGAGAGA CCTCATCAAG CTGGCCGGGG TCACCCTGGG AAAAAGGCGA
    GCCGAGAGGC GGCAGACCAT CCGGCAACCC GCCAAAGAGA AGGACAAGGG CCCCACCAAG
    GCCACCACCA CCAAGCTGGT CTACCAGATC TTTGACACTT TCTTTGCGGA GCAAATTGAA
    AAAGATGACA AGGAAGACAA GGAGAATGCC TTCAAGCGCC GGCGCTGTGG CGTCTGTGAG
    ATTTGTCAAC AGCCCGAGTG TGGAAAGTGT AAGGCCTGTA AGGATATGGT TAAATTTGGT
    GGTAGCGGAC GGAGCAAGCA GGCTTGCCAA AAGAGGAGGT GTCCCAACAT GGCCATGAAG
    GAGGCAGACG ATGACGAGGA AGTGGATGAC AATATTCCAG AGATGCCATC ACCCAAAAAG
    ATGCATCAGG GGAAGAAAAA GAAGCAGAAT AAGAATCGGA TCTCTTGGGT TGGCGATGCC
    GTCAAGACTG ACGGGAAGAA GAGTTACTAC AAGAAGGTAT GCATCGACTC GGAAACCCTG
    GAAGTGGGGG ACTGTGTTTC TGTAATTCCA GACGACTCTT CAAAACCACT GTATCTAGCA
    AGGGTCACGG CGCTGTGGGA GGACAGCAGC AATGGGCAGA TGTTCCATGC CCACTGGTTC
    TGTGCTGGGA CGGACACGGT CCTCGGGGCC ACATCGGACC CCCTGGAGCT GTTCCTGGTT
    GACGAGTGTG AGGACATGCA GCTCTCGTAC ATCCACAGCA AGGTGCAGGT CATTTATAAG
    GCGCCCTCAG AGAACTGGGC CATGGAGGGA GGCGTGGACC CCGAGGCCCT GATGTCAGAG
    GACGACGGGA AGACCTACTT CTACCAGCTG TGGTACGACC AAGACTACGC GAGATTTGAG
    TCCCCTCCGA AAACTCAGCC GACGGAGGAC AACAAGTACA AGTTCTGCGC AAGCTGTGCA
    CGTCTGGCCG AAATGAGGCA GAAGGAAATC CCCAGGGTCG TGGAGCAGCT CCAGGACCTG
    GAAGGCCGCG TCCTCTACAG CCTCGCCACC AAGAACGGCG TCCAGTACCG GGTGGGCGAT
    GGCGTGTACC TCCCTCCCGA GGCCTTCACC TTCAACATCA AGCTGTCCAG TCCTGTGAAA
    CGCCCCCGGA AGGAGCCTGT GGACGAAGCT CTGTATCCAG AACACTACCG GAAGTACTCT
    GACTACATCA AGGGCAGCAA CCTGGATGCC CCTGAGCCCT ACCGTATTGG CCGCATAAAG
    GAGATCTTCT GCAGCAAGAA GAGCAACGGC CGGCCCAATG AGACAGACAT CAAGATCAGG
    GTCAACAAGT TCTACAGGCC GGAGAACACA CACAAGTCTA CCCCAGCCAG TTACCACGCA
    GACATCAACC TGCTTTACTG GAGCGATGAG GAGGCCGTGG TGGACTTCAA GGCCGTGCAG
    GGCCGCTGCA CCGTGGAGTA CGGAGAGGAC CTGCCTCAGT GCCTCCAGGA CTTCTCCGCT
    GGTGGCCCCG ATCGCTTCTA TTTTCTCGAG GCCTATAACG CCAAGAGCAA AAGCTTTGAA
    GATCCTCCGA ACCACGCCCG GAGCACCGGA AATAAAGGGA AAGGGAAGGG GAAAGGAAAA
    AACAGGACGA AATCTCAGAC GTGTGAGCCG AGTGAACTGG AGACAGAAAT CAAACTGCCG
    AAGCTGCGGA CCCTGGACGT GTTTTCCGGC TGTGGGGGAT TGTCGGAAGG CTTCCACCAA
    GCAGGCATCT CGGAGACACT TTGGGCCATC GAGATGTGGG ACCCTGCGGC CCAGGCGTTC
    CGGCTCAACA ACCCTGGGTC CACGGTGTTC ACAGAGGACT GCAACGTCCT GCTGAAGCTG
    GTCATGGCTG GGGAGGTGAC CAACTCCCGC GGCCAGAAGC TGCCTCAGAA GGGAGATGTG
    GAGATGCTGT GCGGCGGGCC GCCCTGCCAG GGCTTCAGCG GCATGAACCG CTTCAACTCT
    CGAACCTACT CCAAATTCAA GAACTCTCTG GTGGTCTCTT TCCTCAGCTA CTGTGACTAC
    TACCGGCCCC GCTACTTCCT CTTGGAGAAC GTTCGGAACT TCGTCTCCTT CAAGCGCTCC
    ATGGTCCTGA AGCTGACGCT GCGCTGCCTG GTCCGCATGG GCTACCAGTG CACCTTTGGC
    GTGCTGCAGG CTGGTCAGTA CGGCGTGGCC CAGACTCGGA GGCGAGCCAT CATCCTGGCT
    GCAGCCCCTG GGGAGCCACT CCCGCTGTTC CCGGAGCCGT TGCATGTGTT CGCACCCCGG
    GCCTGCCAGC TGAGCGTCGT AGTGGACGAC AAGAAGTTTG TCAGCAACAT CACCAGGTTG
    AGCTCGGGTC CCTTCCGAAC CATCACCGTG CGGGACACCA TGTCTGACCT CCCTGAGATC
    CGGAACGGGG CCTCGGCACT GGAGATTTCA TACAACGGGG AGCCCCAGTC CTGGTTCCAG
    AGGCAGCTCC GGGGCTCGCA GTACCAGCCC ATCCTCAGGG ATCATATTTG CAAGGACATG
    AGCGCCTTGG TGGCTGCCCG CATGCGGCAC ATCCCCCTGG CCCCGGGCTC GGACTGGCGT
    GACCTGCCCA ACATTGAGGT GCGGCTCTCT GACGGCACCC TGGCCCGGAA GCTGCGGTAC
    AACTACCACG ACAAGAAGAA CGGCTGCAGC AGCAGCGGCG CCCTCCGTGG GGTCTGCTCC
    TGTGTGGAAG GCAAGCCCTG TGAGCCTGCG GCCCGACAGT TTAACACCCT TATCCCCTGG
    TGCCTGCCCC ACACTGGGAA CAGGCACAAC CACTGGGCCG GCCTCTACGG GCGTCTCGAG
    TGGGACGGCT TCTTCAGCAC AACTGTCACC AACCCCGAGC CCATGGGCAA GCAGGGCCGC
    GTGCTCCACC CCGAGCAGCA CCGAGTGGTG AGCGTCCGGG AGTGCGCCCG CTCCCAGGGC
    TTCCCCGACA CCTATCGGCT GTTCGGCAAC ATCCTAGACA AGCACCGGCA GGTGGGTAAT
    GCTGTGCCGC CGCCACTGGC CAAAGCCATC GGCTTGGAGA TCAAGCGCTG CATGTTGGCC
    AAAGCGCGCG AGAGCGCCTC AGGGCATATA AAAGTCATGT TTGGGGGATT CCTTGATGGC
    TTAGTGGTAA AGAATCTGCC TGCCAGTGCA GGAGACACGG ATTCAATCCC TGATCCAGGA
    AGATCCCACA TGCTGCAGAG CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327481.1
    CDS37..4983
    Translation

    Target ORF information:

    RefSeq Version XM_015471995.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471995.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCAGCCT CCAGAGACAG ATGCCGCCGC CGGTCGGGGC CCTGGCGCGG CCGGCTCAGG 
    CTCAAAGATT TGGAAAGAGA TAGTTTGACA GAAAAGGAAT GTGTGAAGGA GAAACTGAAT
    CTCTTGCACG AATTTCTGCG GACAGAAATA AAGAATCAGT TATGTGATTT GGAAACCAAA
    TTGCATAAAG AAGAATTATC TGAGGAGGGC TACCTGGCTA AAGTCAAATC CCTTTTAAAT
    AAAGATTTGT CCTTGGAGAA CGGAGCTCAT GCTTTCAGTC GGGAAGCGAA TGGATGTCTA
    GAGAACGGGA GCCAGACAAG TGGTGAGGAT TGCAGAGTGG TAATGGCAGA GAAAGGCAAG
    CCCCCCAAAC CTGTCTCCAG ACTTTACACG CCCAGGAGAA GCAAGTCTGA TGGAGAAACA
    AAGTCTGAAG TCTCTTCTAG CCCCAGGATT ACAAGGAAGA CTACCAGGCA GACCACCATC
    ACATCTCATT TCCCACGGGG CCCTGCCAAA CGAAAACCTG AGGAAGAACC TGAAAAAGTG
    AAGTCAGACG ATTCTGTTGA TGAAGAAAAA GACCAGGAGG AAAAGAGACG TCGAGTTACA
    TCCAGAGAAC GAGTTGCTGG GCTGCTCCCT GCAGAAGAAC CAGGAAGAGT AAGACCAGGA
    ACACACATGG AAGAAGAAGG AAGAGATGAT AAAGAAGAAA AGAGACTCAG AAGTCAAACC
    AAAGAACCGA CACCTAAACA CAAAGCTAAG GAGGAGCCAG ACAGAGATGT GAGGCCTGGA
    GGAGCTCAGG CTGAAATGAA TGAAGGAGAA GACAAAGATG AAAAGAGGCA CAGAAGTCAA
    CCCAAAGATC TAGCTAGCAA ACGGAGACCA GAAGAAAAAG AACCTGAAAG AGTAAAGCCA
    CAAGTTTCTG ATGAGAAAGA TGAAGATGAA AAGTTCTGGA GAATACAGTT CACATATCAA
    AGTACCAGCA GGGAGGAGAA GAGACGCAGA ACTACATACA GAGAACTAAC CGAGAAGAAA
    ATGACTCGAA CCAAAATAGC CGTAGTGTCC AAGACCAATC CTCCGAAGTG CACCGAGTGC
    TTGCAGTACC TGGACGACCC TGAGCTGAGA TACGAGCAGC ACCCCCCCGA TGCGGTGGAA
    GAGATACAGA TACTGACCAA CGAGAGGTTG TCCATCTTTG ATGCCAACGA ATCTGGCTTT
    GAGAGTTACG AGGATTTGCC TCAGCACAAA CTAACCTGCT TCAGCGTGTA CTGTAAACGC
    GGTCACCTTT GCCCGATCGA CACCGGCCTC ATTGAGAAGG ATGTCGAGCT CCTCTTTTCT
    GGTTCAGCAA AGCCGATATA TGAGGATGAC CCATCTCCCG AAGGTGGTAT TAATGGCAAA
    AATTTTGGCC CCATAAACGA ATGGTGGATT GCTGGTTTTG ATGGAGGTGA AAAGGCTCTT
    CTTGGCTTTA GCACCTCATT TGCCGAGTAT ATCTTGATGG ATCCCAGCCC AGAGTACGCA
    CCACTATTCA GCGTGATGCA GGAGAAGATC TATATAAGTA AGATAGTGGT TGAGTTCCTG
    CAGAGCAACC CTGACTCCAC CTACGAAGAC CTGATCAATA AGATTGAGAC CACCGTTCCT
    CCTTGTATGC TCAACTTGAA TCGATTCACA GAGGATTCTC TCCTGCGGCA TGCCCAGTTC
    GTGGTGGAGC AAGTAGAGAG TTATGATCGG GCTGGGGACA GTGACGAGCA GCCCATCTTC
    CTGAGCCCCT GCATGAGAGA CCTCATCAAG CTGGCCGGGG TCACCCTGGG AAAAAGGCGA
    GCCGAGAGGC GGCAGACCAT CCGGCAACCC GCCAAAGAGA AGGACAAGGG CCCCACCAAG
    GCCACCACCA CCAAGCTGGT CTACCAGATC TTTGACACTT TCTTTGCGGA GCAAATTGAA
    AAAGATGACA AGGAAGACAA GGAGAATGCC TTCAAGCGCC GGCGCTGTGG CGTCTGTGAG
    ATTTGTCAAC AGCCCGAGTG TGGAAAGTGT AAGGCCTGTA AGGATATGGT TAAATTTGGT
    GGTAGCGGAC GGAGCAAGCA GGCTTGCCAA AAGAGGAGGT GTCCCAACAT GGCCATGAAG
    GAGGCAGACG ATGACGAGGA AGTGGATGAC AATATTCCAG AGATGCCATC ACCCAAAAAG
    ATGCATCAGG GGAAGAAAAA GAAGCAGAAT AAGAATCGGA TCTCTTGGGT TGGCGATGCC
    GTCAAGACTG ACGGGAAGAA GAGTTACTAC AAGAAGGTAT GCATCGACTC GGAAACCCTG
    GAAGTGGGGG ACTGTGTTTC TGTAATTCCA GACGACTCTT CAAAACCACT GTATCTAGCA
    AGGGTCACGG CGCTGTGGGA GGACAGCAGC AATGGGCAGA TGTTCCATGC CCACTGGTTC
    TGTGCTGGGA CGGACACGGT CCTCGGGGCC ACATCGGACC CCCTGGAGCT GTTCCTGGTT
    GACGAGTGTG AGGACATGCA GCTCTCGTAC ATCCACAGCA AGGTGCAGGT CATTTATAAG
    GCGCCCTCAG AGAACTGGGC CATGGAGGGA GGCGTGGACC CCGAGGCCCT GATGTCAGAG
    GACGACGGGA AGACCTACTT CTACCAGCTG TGGTACGACC AAGACTACGC GAGATTTGAG
    TCCCCTCCGA AAACTCAGCC GACGGAGGAC AACAAGTACA AGTTCTGCGC AAGCTGTGCA
    CGTCTGGCCG AAATGAGGCA GAAGGAAATC CCCAGGGTCG TGGAGCAGCT CCAGGACCTG
    GAAGGCCGCG TCCTCTACAG CCTCGCCACC AAGAACGGCG TCCAGTACCG GGTGGGCGAT
    GGCGTGTACC TCCCTCCCGA GGCCTTCACC TTCAACATCA AGCTGTCCAG TCCTGTGAAA
    CGCCCCCGGA AGGAGCCTGT GGACGAAGCT CTGTATCCAG AACACTACCG GAAGTACTCT
    GACTACATCA AGGGCAGCAA CCTGGATGCC CCTGAGCCCT ACCGTATTGG CCGCATAAAG
    GAGATCTTCT GCAGCAAGAA GAGCAACGGC CGGCCCAATG AGACAGACAT CAAGATCAGG
    GTCAACAAGT TCTACAGGCC GGAGAACACA CACAAGTCTA CCCCAGCCAG TTACCACGCA
    GACATCAACC TGCTTTACTG GAGCGATGAG GAGGCCGTGG TGGACTTCAA GGCCGTGCAG
    GGCCGCTGCA CCGTGGAGTA CGGAGAGGAC CTGCCTCAGT GCCTCCAGGA CTTCTCCGCT
    GGTGGCCCCG ATCGCTTCTA TTTTCTCGAG GCCTATAACG CCAAGAGCAA AAGCTTTGAA
    GATCCTCCGA ACCACGCCCG GAGCACCGGA AATAAAGGGA AAGGGAAGGG GAAAGGAAAA
    AACAGGACGA AATCTCAGAC GTGTGAGCCG AGTGAACTGG AGACAGAAAT CAAACTGCCG
    AAGCTGCGGA CCCTGGACGT GTTTTCCGGC TGTGGGGGAT TGTCGGAAGG CTTCCACCAA
    GCAGGCATCT CGGAGACACT TTGGGCCATC GAGATGTGGG ACCCTGCGGC CCAGGCGTTC
    CGGCTCAACA ACCCTGGGTC CACGGTGTTC ACAGAGGACT GCAACGTCCT GCTGAAGCTG
    GTCATGGCTG GGGAGGTGAC CAACTCCCGC GGCCAGAAGC TGCCTCAGAA GGGAGATGTG
    GAGATGCTGT GCGGCGGGCC GCCCTGCCAG GGCTTCAGCG GCATGAACCG CTTCAACTCT
    CGAACCTACT CCAAATTCAA GAACTCTCTG GTGGTCTCTT TCCTCAGCTA CTGTGACTAC
    TACCGGCCCC GCTACTTCCT CTTGGAGAAC GTTCGGAACT TCGTCTCCTT CAAGCGCTCC
    ATGGTCCTGA AGCTGACGCT GCGCTGCCTG GTCCGCATGG GCTACCAGTG CACCTTTGGC
    GTGCTGCAGG CTGGTCAGTA CGGCGTGGCC CAGACTCGGA GGCGAGCCAT CATCCTGGCT
    GCAGCCCCTG GGGAGCCACT CCCGCTGTTC CCGGAGCCGT TGCATGTGTT CGCACCCCGG
    GCCTGCCAGC TGAGCGTCGT AGTGGACGAC AAGAAGTTTG TCAGCAACAT CACCAGGTTG
    AGCTCGGGTC CCTTCCGAAC CATCACCGTG CGGGACACCA TGTCTGACCT CCCTGAGATC
    CGGAACGGGG CCTCGGCACT GGAGATTTCA TACAACGGGG AGCCCCAGTC CTGGTTCCAG
    AGGCAGCTCC GGGGCTCGCA GTACCAGCCC ATCCTCAGGG ATCATATTTG CAAGGACATG
    AGCGCCTTGG TGGCTGCCCG CATGCGGCAC ATCCCCCTGG CCCCGGGCTC GGACTGGCGT
    GACCTGCCCA ACATTGAGGT GCGGCTCTCT GACGGCACCC TGGCCCGGAA GCTGCGGTAC
    AACTACCACG ACAAGAAGAA CGGCTGCAGC AGCAGCGGCG CCCTCCGTGG GGTCTGCTCC
    TGTGTGGAAG GCAAGCCCTG TGAGCCTGCG GCCCGACAGT TTAACACCCT TATCCCCTGG
    TGCCTGCCCC ACACTGGGAA CAGGCACAAC CACTGGGCCG GCCTCTACGG GCGTCTCGAG
    TGGGACGGCT TCTTCAGCAC AACTGTCACC AACCCCGAGC CCATGGGCAA GCAGGGCCGC
    GTGCTCCACC CCGAGCAGCA CCGAGTGGTG AGCGTCCGGG AGTGCGCCCG CTCCCAGGGC
    TTCCCCGACA CCTATCGGCT GTTCGGCAAC ATCCTAGACA AGCACCGGCA GGTGGGTAAT
    GCTGTGCCGC CGCCACTGGC CAAAGCCATC GGCTTGGAGA TCAAGCGCTG CATGTTGGCC
    AAAGCGCGCG AGAGCGCCTC AGGGCATATA AAAGTCATGT TTGGGGGATT CCTTGATGGC
    TTAGTGGTAA AGAATCTGCC TGCCAGTGCA GGAGACACGG ATTCAATCCC TGATCCAGGA
    AGATCCCACA TGCTGCAGAG CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91055
    Clone ID Related Accession (Same CDS sequence) XM_015471995.1 , XM_015471995.2
    Accession Version XM_015471995.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471995.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCAGCCT CCAGAGACAG ATGCCGCCGC CGGTCGGGGC CCTGGCGCGG CCGGCTCAGG 
    CTCAAAGATT TGGAAAGAGA TAGTTTGACA GAAAAGGAAT GTGTGAAGGA GAAACTGAAT
    CTCTTGCACG AATTTCTGCG GACAGAAATA AAGAATCAGT TATGTGATTT GGAAACCAAA
    TTGCATAAAG AAGAATTATC TGAGGAGGGC TACCTGGCTA AAGTCAAATC CCTTTTAAAT
    AAAGATTTGT CCTTGGAGAA CGGAGCTCAT GCTTTCAGTC GGGAAGCGAA TGGATGTCTA
    GAGAACGGGA GCCAGACAAG TGGTGAGGAT TGCAGAGTGG TAATGGCAGA GAAAGGCAAG
    CCCCCCAAAC CTGTCTCCAG ACTTTACACG CCCAGGAGAA GCAAGTCTGA TGGAGAAACA
    AAGTCTGAAG TCTCTTCTAG CCCCAGGATT ACAAGGAAGA CTACCAGGCA GACCACCATC
    ACATCTCATT TCCCACGGGG CCCTGCCAAA CGAAAACCTG AGGAAGAACC TGAAAAAGTG
    AAGTCAGACG ATTCTGTTGA TGAAGAAAAA GACCAGGAGG AAAAGAGACG TCGAGTTACA
    TCCAGAGAAC GAGTTGCTGG GCTGCTCCCT GCAGAAGAAC CAGGAAGAGT AAGACCAGGA
    ACACACATGG AAGAAGAAGG AAGAGATGAT AAAGAAGAAA AGAGACTCAG AAGTCAAACC
    AAAGAACCGA CACCTAAACA CAAAGCTAAG GAGGAGCCAG ACAGAGATGT GAGGCCTGGA
    GGAGCTCAGG CTGAAATGAA TGAAGGAGAA GACAAAGATG AAAAGAGGCA CAGAAGTCAA
    CCCAAAGATC TAGCTAGCAA ACGGAGACCA GAAGAAAAAG AACCTGAAAG AGTAAAGCCA
    CAAGTTTCTG ATGAGAAAGA TGAAGATGAA AAGTTCTGGA GAATACAGTT CACATATCAA
    AGTACCAGCA GGGAGGAGAA GAGACGCAGA ACTACATACA GAGAACTAAC CGAGAAGAAA
    ATGACTCGAA CCAAAATAGC CGTAGTGTCC AAGACCAATC CTCCGAAGTG CACCGAGTGC
    TTGCAGTACC TGGACGACCC TGAGCTGAGA TACGAGCAGC ACCCCCCCGA TGCGGTGGAA
    GAGATACAGA TACTGACCAA CGAGAGGTTG TCCATCTTTG ATGCCAACGA ATCTGGCTTT
    GAGAGTTACG AGGATTTGCC TCAGCACAAA CTAACCTGCT TCAGCGTGTA CTGTAAACGC
    GGTCACCTTT GCCCGATCGA CACCGGCCTC ATTGAGAAGG ATGTCGAGCT CCTCTTTTCT
    GGTTCAGCAA AGCCGATATA TGAGGATGAC CCATCTCCCG AAGGTGGTAT TAATGGCAAA
    AATTTTGGCC CCATAAACGA ATGGTGGATT GCTGGTTTTG ATGGAGGTGA AAAGGCTCTT
    CTTGGCTTTA GCACCTCATT TGCCGAGTAT ATCTTGATGG ATCCCAGCCC AGAGTACGCA
    CCACTATTCA GCGTGATGCA GGAGAAGATC TATATAAGTA AGATAGTGGT TGAGTTCCTG
    CAGAGCAACC CTGACTCCAC CTACGAAGAC CTGATCAATA AGATTGAGAC CACCGTTCCT
    CCTTGTATGC TCAACTTGAA TCGATTCACA GAGGATTCTC TCCTGCGGCA TGCCCAGTTC
    GTGGTGGAGC AAGTAGAGAG TTATGATCGG GCTGGGGACA GTGACGAGCA GCCCATCTTC
    CTGAGCCCCT GCATGAGAGA CCTCATCAAG CTGGCCGGGG TCACCCTGGG AAAAAGGCGA
    GCCGAGAGGC GGCAGACCAT CCGGCAACCC GCCAAAGAGA AGGACAAGGG CCCCACCAAG
    GCCACCACCA CCAAGCTGGT CTACCAGATC TTTGACACTT TCTTTGCGGA GCAAATTGAA
    AAAGATGACA AGGAAGACAA GGAGAATGCC TTCAAGCGCC GGCGCTGTGG CGTCTGTGAG
    ATTTGTCAAC AGCCCGAGTG TGGAAAGTGT AAGGCCTGTA AGGATATGGT TAAATTTGGT
    GGTAGCGGAC GGAGCAAGCA GGCTTGCCAA AAGAGGAGGT GTCCCAACAT GGCCATGAAG
    GAGGCAGACG ATGACGAGGA AGTGGATGAC AATATTCCAG AGATGCCATC ACCCAAAAAG
    ATGCATCAGG GGAAGAAAAA GAAGCAGAAT AAGAATCGGA TCTCTTGGGT TGGCGATGCC
    GTCAAGACTG ACGGGAAGAA GAGTTACTAC AAGAAGGTAT GCATCGACTC GGAAACCCTG
    GAAGTGGGGG ACTGTGTTTC TGTAATTCCA GACGACTCTT CAAAACCACT GTATCTAGCA
    AGGGTCACGG CGCTGTGGGA GGACAGCAGC AATGGGCAGA TGTTCCATGC CCACTGGTTC
    TGTGCTGGGA CGGACACGGT CCTCGGGGCC ACATCGGACC CCCTGGAGCT GTTCCTGGTT
    GACGAGTGTG AGGACATGCA GCTCTCGTAC ATCCACAGCA AGGTGCAGGT CATTTATAAG
    GCGCCCTCAG AGAACTGGGC CATGGAGGGA GGCGTGGACC CCGAGGCCCT GATGTCAGAG
    GACGACGGGA AGACCTACTT CTACCAGCTG TGGTACGACC AAGACTACGC GAGATTTGAG
    TCCCCTCCGA AAACTCAGCC GACGGAGGAC AACAAGTACA AGTTCTGCGC AAGCTGTGCA
    CGTCTGGCCG AAATGAGGCA GAAGGAAATC CCCAGGGTCG TGGAGCAGCT CCAGGACCTG
    GAAGGCCGCG TCCTCTACAG CCTCGCCACC AAGAACGGCG TCCAGTACCG GGTGGGCGAT
    GGCGTGTACC TCCCTCCCGA GGCCTTCACC TTCAACATCA AGCTGTCCAG TCCTGTGAAA
    CGCCCCCGGA AGGAGCCTGT GGACGAAGCT CTGTATCCAG AACACTACCG GAAGTACTCT
    GACTACATCA AGGGCAGCAA CCTGGATGCC CCTGAGCCCT ACCGTATTGG CCGCATAAAG
    GAGATCTTCT GCAGCAAGAA GAGCAACGGC CGGCCCAATG AGACAGACAT CAAGATCAGG
    GTCAACAAGT TCTACAGGCC GGAGAACACA CACAAGTCTA CCCCAGCCAG TTACCACGCA
    GACATCAACC TGCTTTACTG GAGCGATGAG GAGGCCGTGG TGGACTTCAA GGCCGTGCAG
    GGCCGCTGCA CCGTGGAGTA CGGAGAGGAC CTGCCTCAGT GCCTCCAGGA CTTCTCCGCT
    GGTGGCCCCG ATCGCTTCTA TTTTCTCGAG GCCTATAACG CCAAGAGCAA AAGCTTTGAA
    GATCCTCCGA ACCACGCCCG GAGCACCGGA AATAAAGGGA AAGGGAAGGG GAAAGGAAAA
    AACAGGACGA AATCTCAGAC GTGTGAGCCG AGTGAACTGG AGACAGAAAT CAAACTGCCG
    AAGCTGCGGA CCCTGGACGT GTTTTCCGGC TGTGGGGGAT TGTCGGAAGG CTTCCACCAA
    GCAGGCATCT CGGAGACACT TTGGGCCATC GAGATGTGGG ACCCTGCGGC CCAGGCGTTC
    CGGCTCAACA ACCCTGGGTC CACGGTGTTC ACAGAGGACT GCAACGTCCT GCTGAAGCTG
    GTCATGGCTG GGGAGGTGAC CAACTCCCGC GGCCAGAAGC TGCCTCAGAA GGGAGATGTG
    GAGATGCTGT GCGGCGGGCC GCCCTGCCAG GGCTTCAGCG GCATGAACCG CTTCAACTCT
    CGAACCTACT CCAAATTCAA GAACTCTCTG GTGGTCTCTT TCCTCAGCTA CTGTGACTAC
    TACCGGCCCC GCTACTTCCT CTTGGAGAAC GTTCGGAACT TCGTCTCCTT CAAGCGCTCC
    ATGGTCCTGA AGCTGACGCT GCGCTGCCTG GTCCGCATGG GCTACCAGTG CACCTTTGGC
    GTGCTGCAGG CTGGTCAGTA CGGCGTGGCC CAGACTCGGA GGCGAGCCAT CATCCTGGCT
    GCAGCCCCTG GGGAGCCACT CCCGCTGTTC CCGGAGCCGT TGCATGTGTT CGCACCCCGG
    GCCTGCCAGC TGAGCGTCGT AGTGGACGAC AAGAAGTTTG TCAGCAACAT CACCAGGTTG
    AGCTCGGGTC CCTTCCGAAC CATCACCGTG CGGGACACCA TGTCTGACCT CCCTGAGATC
    CGGAACGGGG CCTCGGCACT GGAGATTTCA TACAACGGGG AGCCCCAGTC CTGGTTCCAG
    AGGCAGCTCC GGGGCTCGCA GTACCAGCCC ATCCTCAGGG ATCATATTTG CAAGGACATG
    AGCGCCTTGG TGGCTGCCCG CATGCGGCAC ATCCCCCTGG CCCCGGGCTC GGACTGGCGT
    GACCTGCCCA ACATTGAGGT GCGGCTCTCT GACGGCACCC TGGCCCGGAA GCTGCGGTAC
    AACTACCACG ACAAGAAGAA CGGCTGCAGC AGCAGCGGCG CCCTCCGTGG GGTCTGCTCC
    TGTGTGGAAG GCAAGCCCTG TGAGCCTGCG GCCCGACAGT TTAACACCCT TATCCCCTGG
    TGCCTGCCCC ACACTGGGAA CAGGCACAAC CACTGGGCCG GCCTCTACGG GCGTCTCGAG
    TGGGACGGCT TCTTCAGCAC AACTGTCACC AACCCCGAGC CCATGGGCAA GCAGGGCCGC
    GTGCTCCACC CCGAGCAGCA CCGAGTGGTG AGCGTCCGGG AGTGCGCCCG CTCCCAGGGC
    TTCCCCGACA CCTATCGGCT GTTCGGCAAC ATCCTAGACA AGCACCGGCA GGTGGGTAAT
    GCTGTGCCGC CGCCACTGGC CAAAGCCATC GGCTTGGAGA TCAAGCGCTG CATGTTGGCC
    AAAGCGCGCG AGAGCGCCTC AGGGCATATA AAAGTCATGT TTGGGGGATT CCTTGATGGC
    TTAGTGGTAA AGAATCTGCC TGCCAGTGCA GGAGACACGG ATTCAATCCC TGATCCAGGA
    AGATCCCACA TGCTGCAGAG CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327481.1
    CDS86..5032
    Translation

    Target ORF information:

    RefSeq Version XM_015471995.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471995.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCAGCCT CCAGAGACAG ATGCCGCCGC CGGTCGGGGC CCTGGCGCGG CCGGCTCAGG 
    CTCAAAGATT TGGAAAGAGA TAGTTTGACA GAAAAGGAAT GTGTGAAGGA GAAACTGAAT
    CTCTTGCACG AATTTCTGCG GACAGAAATA AAGAATCAGT TATGTGATTT GGAAACCAAA
    TTGCATAAAG AAGAATTATC TGAGGAGGGC TACCTGGCTA AAGTCAAATC CCTTTTAAAT
    AAAGATTTGT CCTTGGAGAA CGGAGCTCAT GCTTTCAGTC GGGAAGCGAA TGGATGTCTA
    GAGAACGGGA GCCAGACAAG TGGTGAGGAT TGCAGAGTGG TAATGGCAGA GAAAGGCAAG
    CCCCCCAAAC CTGTCTCCAG ACTTTACACG CCCAGGAGAA GCAAGTCTGA TGGAGAAACA
    AAGTCTGAAG TCTCTTCTAG CCCCAGGATT ACAAGGAAGA CTACCAGGCA GACCACCATC
    ACATCTCATT TCCCACGGGG CCCTGCCAAA CGAAAACCTG AGGAAGAACC TGAAAAAGTG
    AAGTCAGACG ATTCTGTTGA TGAAGAAAAA GACCAGGAGG AAAAGAGACG TCGAGTTACA
    TCCAGAGAAC GAGTTGCTGG GCTGCTCCCT GCAGAAGAAC CAGGAAGAGT AAGACCAGGA
    ACACACATGG AAGAAGAAGG AAGAGATGAT AAAGAAGAAA AGAGACTCAG AAGTCAAACC
    AAAGAACCGA CACCTAAACA CAAAGCTAAG GAGGAGCCAG ACAGAGATGT GAGGCCTGGA
    GGAGCTCAGG CTGAAATGAA TGAAGGAGAA GACAAAGATG AAAAGAGGCA CAGAAGTCAA
    CCCAAAGATC TAGCTAGCAA ACGGAGACCA GAAGAAAAAG AACCTGAAAG AGTAAAGCCA
    CAAGTTTCTG ATGAGAAAGA TGAAGATGAA AAGTTCTGGA GAATACAGTT CACATATCAA
    AGTACCAGCA GGGAGGAGAA GAGACGCAGA ACTACATACA GAGAACTAAC CGAGAAGAAA
    ATGACTCGAA CCAAAATAGC CGTAGTGTCC AAGACCAATC CTCCGAAGTG CACCGAGTGC
    TTGCAGTACC TGGACGACCC TGAGCTGAGA TACGAGCAGC ACCCCCCCGA TGCGGTGGAA
    GAGATACAGA TACTGACCAA CGAGAGGTTG TCCATCTTTG ATGCCAACGA ATCTGGCTTT
    GAGAGTTACG AGGATTTGCC TCAGCACAAA CTAACCTGCT TCAGCGTGTA CTGTAAACGC
    GGTCACCTTT GCCCGATCGA CACCGGCCTC ATTGAGAAGG ATGTCGAGCT CCTCTTTTCT
    GGTTCAGCAA AGCCGATATA TGAGGATGAC CCATCTCCCG AAGGTGGTAT TAATGGCAAA
    AATTTTGGCC CCATAAACGA ATGGTGGATT GCTGGTTTTG ATGGAGGTGA AAAGGCTCTT
    CTTGGCTTTA GCACCTCATT TGCCGAGTAT ATCTTGATGG ATCCCAGCCC AGAGTACGCA
    CCACTATTCA GCGTGATGCA GGAGAAGATC TATATAAGTA AGATAGTGGT TGAGTTCCTG
    CAGAGCAACC CTGACTCCAC CTACGAAGAC CTGATCAATA AGATTGAGAC CACCGTTCCT
    CCTTGTATGC TCAACTTGAA TCGATTCACA GAGGATTCTC TCCTGCGGCA TGCCCAGTTC
    GTGGTGGAGC AAGTAGAGAG TTATGATCGG GCTGGGGACA GTGACGAGCA GCCCATCTTC
    CTGAGCCCCT GCATGAGAGA CCTCATCAAG CTGGCCGGGG TCACCCTGGG AAAAAGGCGA
    GCCGAGAGGC GGCAGACCAT CCGGCAACCC GCCAAAGAGA AGGACAAGGG CCCCACCAAG
    GCCACCACCA CCAAGCTGGT CTACCAGATC TTTGACACTT TCTTTGCGGA GCAAATTGAA
    AAAGATGACA AGGAAGACAA GGAGAATGCC TTCAAGCGCC GGCGCTGTGG CGTCTGTGAG
    ATTTGTCAAC AGCCCGAGTG TGGAAAGTGT AAGGCCTGTA AGGATATGGT TAAATTTGGT
    GGTAGCGGAC GGAGCAAGCA GGCTTGCCAA AAGAGGAGGT GTCCCAACAT GGCCATGAAG
    GAGGCAGACG ATGACGAGGA AGTGGATGAC AATATTCCAG AGATGCCATC ACCCAAAAAG
    ATGCATCAGG GGAAGAAAAA GAAGCAGAAT AAGAATCGGA TCTCTTGGGT TGGCGATGCC
    GTCAAGACTG ACGGGAAGAA GAGTTACTAC AAGAAGGTAT GCATCGACTC GGAAACCCTG
    GAAGTGGGGG ACTGTGTTTC TGTAATTCCA GACGACTCTT CAAAACCACT GTATCTAGCA
    AGGGTCACGG CGCTGTGGGA GGACAGCAGC AATGGGCAGA TGTTCCATGC CCACTGGTTC
    TGTGCTGGGA CGGACACGGT CCTCGGGGCC ACATCGGACC CCCTGGAGCT GTTCCTGGTT
    GACGAGTGTG AGGACATGCA GCTCTCGTAC ATCCACAGCA AGGTGCAGGT CATTTATAAG
    GCGCCCTCAG AGAACTGGGC CATGGAGGGA GGCGTGGACC CCGAGGCCCT GATGTCAGAG
    GACGACGGGA AGACCTACTT CTACCAGCTG TGGTACGACC AAGACTACGC GAGATTTGAG
    TCCCCTCCGA AAACTCAGCC GACGGAGGAC AACAAGTACA AGTTCTGCGC AAGCTGTGCA
    CGTCTGGCCG AAATGAGGCA GAAGGAAATC CCCAGGGTCG TGGAGCAGCT CCAGGACCTG
    GAAGGCCGCG TCCTCTACAG CCTCGCCACC AAGAACGGCG TCCAGTACCG GGTGGGCGAT
    GGCGTGTACC TCCCTCCCGA GGCCTTCACC TTCAACATCA AGCTGTCCAG TCCTGTGAAA
    CGCCCCCGGA AGGAGCCTGT GGACGAAGCT CTGTATCCAG AACACTACCG GAAGTACTCT
    GACTACATCA AGGGCAGCAA CCTGGATGCC CCTGAGCCCT ACCGTATTGG CCGCATAAAG
    GAGATCTTCT GCAGCAAGAA GAGCAACGGC CGGCCCAATG AGACAGACAT CAAGATCAGG
    GTCAACAAGT TCTACAGGCC GGAGAACACA CACAAGTCTA CCCCAGCCAG TTACCACGCA
    GACATCAACC TGCTTTACTG GAGCGATGAG GAGGCCGTGG TGGACTTCAA GGCCGTGCAG
    GGCCGCTGCA CCGTGGAGTA CGGAGAGGAC CTGCCTCAGT GCCTCCAGGA CTTCTCCGCT
    GGTGGCCCCG ATCGCTTCTA TTTTCTCGAG GCCTATAACG CCAAGAGCAA AAGCTTTGAA
    GATCCTCCGA ACCACGCCCG GAGCACCGGA AATAAAGGGA AAGGGAAGGG GAAAGGAAAA
    AACAGGACGA AATCTCAGAC GTGTGAGCCG AGTGAACTGG AGACAGAAAT CAAACTGCCG
    AAGCTGCGGA CCCTGGACGT GTTTTCCGGC TGTGGGGGAT TGTCGGAAGG CTTCCACCAA
    GCAGGCATCT CGGAGACACT TTGGGCCATC GAGATGTGGG ACCCTGCGGC CCAGGCGTTC
    CGGCTCAACA ACCCTGGGTC CACGGTGTTC ACAGAGGACT GCAACGTCCT GCTGAAGCTG
    GTCATGGCTG GGGAGGTGAC CAACTCCCGC GGCCAGAAGC TGCCTCAGAA GGGAGATGTG
    GAGATGCTGT GCGGCGGGCC GCCCTGCCAG GGCTTCAGCG GCATGAACCG CTTCAACTCT
    CGAACCTACT CCAAATTCAA GAACTCTCTG GTGGTCTCTT TCCTCAGCTA CTGTGACTAC
    TACCGGCCCC GCTACTTCCT CTTGGAGAAC GTTCGGAACT TCGTCTCCTT CAAGCGCTCC
    ATGGTCCTGA AGCTGACGCT GCGCTGCCTG GTCCGCATGG GCTACCAGTG CACCTTTGGC
    GTGCTGCAGG CTGGTCAGTA CGGCGTGGCC CAGACTCGGA GGCGAGCCAT CATCCTGGCT
    GCAGCCCCTG GGGAGCCACT CCCGCTGTTC CCGGAGCCGT TGCATGTGTT CGCACCCCGG
    GCCTGCCAGC TGAGCGTCGT AGTGGACGAC AAGAAGTTTG TCAGCAACAT CACCAGGTTG
    AGCTCGGGTC CCTTCCGAAC CATCACCGTG CGGGACACCA TGTCTGACCT CCCTGAGATC
    CGGAACGGGG CCTCGGCACT GGAGATTTCA TACAACGGGG AGCCCCAGTC CTGGTTCCAG
    AGGCAGCTCC GGGGCTCGCA GTACCAGCCC ATCCTCAGGG ATCATATTTG CAAGGACATG
    AGCGCCTTGG TGGCTGCCCG CATGCGGCAC ATCCCCCTGG CCCCGGGCTC GGACTGGCGT
    GACCTGCCCA ACATTGAGGT GCGGCTCTCT GACGGCACCC TGGCCCGGAA GCTGCGGTAC
    AACTACCACG ACAAGAAGAA CGGCTGCAGC AGCAGCGGCG CCCTCCGTGG GGTCTGCTCC
    TGTGTGGAAG GCAAGCCCTG TGAGCCTGCG GCCCGACAGT TTAACACCCT TATCCCCTGG
    TGCCTGCCCC ACACTGGGAA CAGGCACAAC CACTGGGCCG GCCTCTACGG GCGTCTCGAG
    TGGGACGGCT TCTTCAGCAC AACTGTCACC AACCCCGAGC CCATGGGCAA GCAGGGCCGC
    GTGCTCCACC CCGAGCAGCA CCGAGTGGTG AGCGTCCGGG AGTGCGCCCG CTCCCAGGGC
    TTCCCCGACA CCTATCGGCT GTTCGGCAAC ATCCTAGACA AGCACCGGCA GGTGGGTAAT
    GCTGTGCCGC CGCCACTGGC CAAAGCCATC GGCTTGGAGA TCAAGCGCTG CATGTTGGCC
    AAAGCGCGCG AGAGCGCCTC AGGGCATATA AAAGTCATGT TTGGGGGATT CCTTGATGGC
    TTAGTGGTAA AGAATCTGCC TGCCAGTGCA GGAGACACGG ATTCAATCCC TGATCCAGGA
    AGATCCCACA TGCTGCAGAG CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91056
    Clone ID Related Accession (Same CDS sequence) XM_015471996.1
    Accession Version XM_015471996.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGATCTT GGACCAGAAG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA 
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGGGCATAT AAAAGTCATG
    TTTGGGGGAT TCCTTGATGG CTTAGTGGTA AAGAATCTGC CTGCCAGTGC AGGAGACACG
    GATTCAATCC CTGATCCAGG AAGATCCCAC ATGCTGCAGA GCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327482.1
    CDS96..5003
    Translation

    Target ORF information:

    RefSeq Version XM_015471996.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471996.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGATCTT GGACCAGAAG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA 
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGGGCATAT AAAAGTCATG
    TTTGGGGGAT TCCTTGATGG CTTAGTGGTA AAGAATCTGC CTGCCAGTGC AGGAGACACG
    GATTCAATCC CTGATCCAGG AAGATCCCAC ATGCTGCAGA GCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91057
    Clone ID Related Accession (Same CDS sequence) XM_015471997.1
    Accession Version XM_015471997.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCAGAGA AAGGCAAGCC CCCCAAACCT GTCTCCAGAC TTTACACGCC CAGGAGAAGC 
    AAGTCTGATG GAGAAACAAA GTCTGAAGTC TCTTCTAGCC CCAGGATTAC AAGGAAGACT
    ACCAGGCAGA CCACCATCAC ATCTCATTTC CCACGGGGCC CTGCCAAACG AAAACCTGAG
    GAAGAACCTG AAAAAGTGAA GTCAGACGAT TCTGTTGATG AAGAAAAAGA CCAGGAGGAA
    AAGAGACGTC GAGTTACATC CAGAGAACGA GTTGCTGGGC TGCTCCCTGC AGAAGAACCA
    GGAAGAGTAA GACCAGGAAC ACACATGGAA GAAGAAGGAA GAGATGATAA AGAAGAAAAG
    AGACTCAGAA GTCAAACCAA AGAACCGACA CCTAAACACA AAGCTAAGGA GGAGCCAGAC
    AGAGATGTGA GGCCTGGAGG AGCTCAGGCT GAAATGAATG AAGGAGAAGA CAAAGATGAA
    AAGAGGCACA GAAGTCAACC CAAAGATCTA GCTAGCAAAC GGAGACCAGA AGAAAAAGAA
    CCTGAAAGAG TAAAGCCACA AGTTTCTGAT GAGAAAGATG AAGATGAAAA GTTCTGGAGA
    ATACAGTTCA CATATCAAAG TACCAGCAGG GAGGAGAAGA GACGCAGAAC TACATACAGA
    GAACTAACCG AGAAGAAAAT GACTCGAACC AAAATAGCCG TAGTGTCCAA GACCAATCCT
    CCGAAGTGCA CCGAGTGCTT GCAGTACCTG GACGACCCTG AGCTGAGATA CGAGCAGCAC
    CCCCCCGATG CGGTGGAAGA GATACAGATA CTGACCAACG AGAGGTTGTC CATCTTTGAT
    GCCAACGAAT CTGGCTTTGA GAGTTACGAG GATTTGCCTC AGCACAAACT AACCTGCTTC
    AGCGTGTACT GTAAACGCGG TCACCTTTGC CCGATCGACA CCGGCCTCAT TGAGAAGGAT
    GTCGAGCTCC TCTTTTCTGG TTCAGCAAAG CCGATATATG AGGATGACCC ATCTCCCGAA
    GGTGGTATTA ATGGCAAAAA TTTTGGCCCC ATAAACGAAT GGTGGATTGC TGGTTTTGAT
    GGAGGTGAAA AGGCTCTTCT TGGCTTTAGC ACCTCATTTG CCGAGTATAT CTTGATGGAT
    CCCAGCCCAG AGTACGCACC ACTATTCAGC GTGATGCAGG AGAAGATCTA TATAAGTAAG
    ATAGTGGTTG AGTTCCTGCA GAGCAACCCT GACTCCACCT ACGAAGACCT GATCAATAAG
    ATTGAGACCA CCGTTCCTCC TTGTATGCTC AACTTGAATC GATTCACAGA GGATTCTCTC
    CTGCGGCATG CCCAGTTCGT GGTGGAGCAA GTAGAGAGTT ATGATCGGGC TGGGGACAGT
    GACGAGCAGC CCATCTTCCT GAGCCCCTGC ATGAGAGACC TCATCAAGCT GGCCGGGGTC
    ACCCTGGGAA AAAGGCGAGC CGAGAGGCGG CAGACCATCC GGCAACCCGC CAAAGAGAAG
    GACAAGGGCC CCACCAAGGC CACCACCACC AAGCTGGTCT ACCAGATCTT TGACACTTTC
    TTTGCGGAGC AAATTGAAAA AGATGACAAG GAAGACAAGG AGAATGCCTT CAAGCGCCGG
    CGCTGTGGCG TCTGTGAGAT TTGTCAACAG CCCGAGTGTG GAAAGTGTAA GGCCTGTAAG
    GATATGGTTA AATTTGGTGG TAGCGGACGG AGCAAGCAGG CTTGCCAAAA GAGGAGGTGT
    CCCAACATGG CCATGAAGGA GGCAGACGAT GACGAGGAAG TGGATGACAA TATTCCAGAG
    ATGCCATCAC CCAAAAAGAT GCATCAGGGG AAGAAAAAGA AGCAGAATAA GAATCGGATC
    TCTTGGGTTG GCGATGCCGT CAAGACTGAC GGGAAGAAGA GTTACTACAA GAAGGTATGC
    ATCGACTCGG AAACCCTGGA AGTGGGGGAC TGTGTTTCTG TAATTCCAGA CGACTCTTCA
    AAACCACTGT ATCTAGCAAG GGTCACGGCG CTGTGGGAGG ACAGCAGCAA TGGGCAGATG
    TTCCATGCCC ACTGGTTCTG TGCTGGGACG GACACGGTCC TCGGGGCCAC ATCGGACCCC
    CTGGAGCTGT TCCTGGTTGA CGAGTGTGAG GACATGCAGC TCTCGTACAT CCACAGCAAG
    GTGCAGGTCA TTTATAAGGC GCCCTCAGAG AACTGGGCCA TGGAGGGAGG CGTGGACCCC
    GAGGCCCTGA TGTCAGAGGA CGACGGGAAG ACCTACTTCT ACCAGCTGTG GTACGACCAA
    GACTACGCGA GATTTGAGTC CCCTCCGAAA ACTCAGCCGA CGGAGGACAA CAAGTACAAG
    TTCTGCGCAA GCTGTGCACG TCTGGCCGAA ATGAGGCAGA AGGAAATCCC CAGGGTCGTG
    GAGCAGCTCC AGGACCTGGA AGGCCGCGTC CTCTACAGCC TCGCCACCAA GAACGGCGTC
    CAGTACCGGG TGGGCGATGG CGTGTACCTC CCTCCCGAGG CCTTCACCTT CAACATCAAG
    CTGTCCAGTC CTGTGAAACG CCCCCGGAAG GAGCCTGTGG ACGAAGCTCT GTATCCAGAA
    CACTACCGGA AGTACTCTGA CTACATCAAG GGCAGCAACC TGGATGCCCC TGAGCCCTAC
    CGTATTGGCC GCATAAAGGA GATCTTCTGC AGCAAGAAGA GCAACGGCCG GCCCAATGAG
    ACAGACATCA AGATCAGGGT CAACAAGTTC TACAGGCCGG AGAACACACA CAAGTCTACC
    CCAGCCAGTT ACCACGCAGA CATCAACCTG CTTTACTGGA GCGATGAGGA GGCCGTGGTG
    GACTTCAAGG CCGTGCAGGG CCGCTGCACC GTGGAGTACG GAGAGGACCT GCCTCAGTGC
    CTCCAGGACT TCTCCGCTGG TGGCCCCGAT CGCTTCTATT TTCTCGAGGC CTATAACGCC
    AAGAGCAAAA GCTTTGAAGA TCCTCCGAAC CACGCCCGGA GCACCGGAAA TAAAGGGAAA
    GGGAAGGGGA AAGGAAAAAA CAGGACGAAA TCTCAGACGT GTGAGCCGAG TGAACTGGAG
    ACAGAAATCA AACTGCCGAA GCTGCGGACC CTGGACGTGT TTTCCGGCTG TGGGGGATTG
    TCGGAAGGCT TCCACCAAGC AGGCATCTCG GAGACACTTT GGGCCATCGA GATGTGGGAC
    CCTGCGGCCC AGGCGTTCCG GCTCAACAAC CCTGGGTCCA CGGTGTTCAC AGAGGACTGC
    AACGTCCTGC TGAAGCTGGT CATGGCTGGG GAGGTGACCA ACTCCCGCGG CCAGAAGCTG
    CCTCAGAAGG GAGATGTGGA GATGCTGTGC GGCGGGCCGC CCTGCCAGGG CTTCAGCGGC
    ATGAACCGCT TCAACTCTCG AACCTACTCC AAATTCAAGA ACTCTCTGGT GGTCTCTTTC
    CTCAGCTACT GTGACTACTA CCGGCCCCGC TACTTCCTCT TGGAGAACGT TCGGAACTTC
    GTCTCCTTCA AGCGCTCCAT GGTCCTGAAG CTGACGCTGC GCTGCCTGGT CCGCATGGGC
    TACCAGTGCA CCTTTGGCGT GCTGCAGGCT GGTCAGTACG GCGTGGCCCA GACTCGGAGG
    CGAGCCATCA TCCTGGCTGC AGCCCCTGGG GAGCCACTCC CGCTGTTCCC GGAGCCGTTG
    CATGTGTTCG CACCCCGGGC CTGCCAGCTG AGCGTCGTAG TGGACGACAA GAAGTTTGTC
    AGCAACATCA CCAGGTTGAG CTCGGGTCCC TTCCGAACCA TCACCGTGCG GGACACCATG
    TCTGACCTCC CTGAGATCCG GAACGGGGCC TCGGCACTGG AGATTTCATA CAACGGGGAG
    CCCCAGTCCT GGTTCCAGAG GCAGCTCCGG GGCTCGCAGT ACCAGCCCAT CCTCAGGGAT
    CATATTTGCA AGGACATGAG CGCCTTGGTG GCTGCCCGCA TGCGGCACAT CCCCCTGGCC
    CCGGGCTCGG ACTGGCGTGA CCTGCCCAAC ATTGAGGTGC GGCTCTCTGA CGGCACCCTG
    GCCCGGAAGC TGCGGTACAA CTACCACGAC AAGAAGAACG GCTGCAGCAG CAGCGGCGCC
    CTCCGTGGGG TCTGCTCCTG TGTGGAAGGC AAGCCCTGTG AGCCTGCGGC CCGACAGTTT
    AACACCCTTA TCCCCTGGTG CCTGCCCCAC ACTGGGAACA GGCACAACCA CTGGGCCGGC
    CTCTACGGGC GTCTCGAGTG GGACGGCTTC TTCAGCACAA CTGTCACCAA CCCCGAGCCC
    ATGGGCAAGC AGGGCCGCGT GCTCCACCCC GAGCAGCACC GAGTGGTGAG CGTCCGGGAG
    TGCGCCCGCT CCCAGGGCTT CCCCGACACC TATCGGCTGT TCGGCAACAT CCTAGACAAG
    CACCGGCAGG TGGGTAATGC TGTGCCGCCG CCACTGGCCA AAGCCATCGG CTTGGAGATC
    AAGCGCTGCA TGTTGGCCAA AGCGCGCGAG AGCGCCTCAG GGCATATAAA AGTCATGTTT
    GGGGGATTCC TTGATGGCTT AGTGGTAAAG AATCTGCCTG CCAGTGCAGG AGACACGGAT
    TCAATCCCTG ATCCAGGAAG ATCCCACATG CTGCAGAGCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327483.1
    CDS303..4907
    Translation

    Target ORF information:

    RefSeq Version XM_015471997.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471997.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCAGAGA AAGGCAAGCC CCCCAAACCT GTCTCCAGAC TTTACACGCC CAGGAGAAGC 
    AAGTCTGATG GAGAAACAAA GTCTGAAGTC TCTTCTAGCC CCAGGATTAC AAGGAAGACT
    ACCAGGCAGA CCACCATCAC ATCTCATTTC CCACGGGGCC CTGCCAAACG AAAACCTGAG
    GAAGAACCTG AAAAAGTGAA GTCAGACGAT TCTGTTGATG AAGAAAAAGA CCAGGAGGAA
    AAGAGACGTC GAGTTACATC CAGAGAACGA GTTGCTGGGC TGCTCCCTGC AGAAGAACCA
    GGAAGAGTAA GACCAGGAAC ACACATGGAA GAAGAAGGAA GAGATGATAA AGAAGAAAAG
    AGACTCAGAA GTCAAACCAA AGAACCGACA CCTAAACACA AAGCTAAGGA GGAGCCAGAC
    AGAGATGTGA GGCCTGGAGG AGCTCAGGCT GAAATGAATG AAGGAGAAGA CAAAGATGAA
    AAGAGGCACA GAAGTCAACC CAAAGATCTA GCTAGCAAAC GGAGACCAGA AGAAAAAGAA
    CCTGAAAGAG TAAAGCCACA AGTTTCTGAT GAGAAAGATG AAGATGAAAA GTTCTGGAGA
    ATACAGTTCA CATATCAAAG TACCAGCAGG GAGGAGAAGA GACGCAGAAC TACATACAGA
    GAACTAACCG AGAAGAAAAT GACTCGAACC AAAATAGCCG TAGTGTCCAA GACCAATCCT
    CCGAAGTGCA CCGAGTGCTT GCAGTACCTG GACGACCCTG AGCTGAGATA CGAGCAGCAC
    CCCCCCGATG CGGTGGAAGA GATACAGATA CTGACCAACG AGAGGTTGTC CATCTTTGAT
    GCCAACGAAT CTGGCTTTGA GAGTTACGAG GATTTGCCTC AGCACAAACT AACCTGCTTC
    AGCGTGTACT GTAAACGCGG TCACCTTTGC CCGATCGACA CCGGCCTCAT TGAGAAGGAT
    GTCGAGCTCC TCTTTTCTGG TTCAGCAAAG CCGATATATG AGGATGACCC ATCTCCCGAA
    GGTGGTATTA ATGGCAAAAA TTTTGGCCCC ATAAACGAAT GGTGGATTGC TGGTTTTGAT
    GGAGGTGAAA AGGCTCTTCT TGGCTTTAGC ACCTCATTTG CCGAGTATAT CTTGATGGAT
    CCCAGCCCAG AGTACGCACC ACTATTCAGC GTGATGCAGG AGAAGATCTA TATAAGTAAG
    ATAGTGGTTG AGTTCCTGCA GAGCAACCCT GACTCCACCT ACGAAGACCT GATCAATAAG
    ATTGAGACCA CCGTTCCTCC TTGTATGCTC AACTTGAATC GATTCACAGA GGATTCTCTC
    CTGCGGCATG CCCAGTTCGT GGTGGAGCAA GTAGAGAGTT ATGATCGGGC TGGGGACAGT
    GACGAGCAGC CCATCTTCCT GAGCCCCTGC ATGAGAGACC TCATCAAGCT GGCCGGGGTC
    ACCCTGGGAA AAAGGCGAGC CGAGAGGCGG CAGACCATCC GGCAACCCGC CAAAGAGAAG
    GACAAGGGCC CCACCAAGGC CACCACCACC AAGCTGGTCT ACCAGATCTT TGACACTTTC
    TTTGCGGAGC AAATTGAAAA AGATGACAAG GAAGACAAGG AGAATGCCTT CAAGCGCCGG
    CGCTGTGGCG TCTGTGAGAT TTGTCAACAG CCCGAGTGTG GAAAGTGTAA GGCCTGTAAG
    GATATGGTTA AATTTGGTGG TAGCGGACGG AGCAAGCAGG CTTGCCAAAA GAGGAGGTGT
    CCCAACATGG CCATGAAGGA GGCAGACGAT GACGAGGAAG TGGATGACAA TATTCCAGAG
    ATGCCATCAC CCAAAAAGAT GCATCAGGGG AAGAAAAAGA AGCAGAATAA GAATCGGATC
    TCTTGGGTTG GCGATGCCGT CAAGACTGAC GGGAAGAAGA GTTACTACAA GAAGGTATGC
    ATCGACTCGG AAACCCTGGA AGTGGGGGAC TGTGTTTCTG TAATTCCAGA CGACTCTTCA
    AAACCACTGT ATCTAGCAAG GGTCACGGCG CTGTGGGAGG ACAGCAGCAA TGGGCAGATG
    TTCCATGCCC ACTGGTTCTG TGCTGGGACG GACACGGTCC TCGGGGCCAC ATCGGACCCC
    CTGGAGCTGT TCCTGGTTGA CGAGTGTGAG GACATGCAGC TCTCGTACAT CCACAGCAAG
    GTGCAGGTCA TTTATAAGGC GCCCTCAGAG AACTGGGCCA TGGAGGGAGG CGTGGACCCC
    GAGGCCCTGA TGTCAGAGGA CGACGGGAAG ACCTACTTCT ACCAGCTGTG GTACGACCAA
    GACTACGCGA GATTTGAGTC CCCTCCGAAA ACTCAGCCGA CGGAGGACAA CAAGTACAAG
    TTCTGCGCAA GCTGTGCACG TCTGGCCGAA ATGAGGCAGA AGGAAATCCC CAGGGTCGTG
    GAGCAGCTCC AGGACCTGGA AGGCCGCGTC CTCTACAGCC TCGCCACCAA GAACGGCGTC
    CAGTACCGGG TGGGCGATGG CGTGTACCTC CCTCCCGAGG CCTTCACCTT CAACATCAAG
    CTGTCCAGTC CTGTGAAACG CCCCCGGAAG GAGCCTGTGG ACGAAGCTCT GTATCCAGAA
    CACTACCGGA AGTACTCTGA CTACATCAAG GGCAGCAACC TGGATGCCCC TGAGCCCTAC
    CGTATTGGCC GCATAAAGGA GATCTTCTGC AGCAAGAAGA GCAACGGCCG GCCCAATGAG
    ACAGACATCA AGATCAGGGT CAACAAGTTC TACAGGCCGG AGAACACACA CAAGTCTACC
    CCAGCCAGTT ACCACGCAGA CATCAACCTG CTTTACTGGA GCGATGAGGA GGCCGTGGTG
    GACTTCAAGG CCGTGCAGGG CCGCTGCACC GTGGAGTACG GAGAGGACCT GCCTCAGTGC
    CTCCAGGACT TCTCCGCTGG TGGCCCCGAT CGCTTCTATT TTCTCGAGGC CTATAACGCC
    AAGAGCAAAA GCTTTGAAGA TCCTCCGAAC CACGCCCGGA GCACCGGAAA TAAAGGGAAA
    GGGAAGGGGA AAGGAAAAAA CAGGACGAAA TCTCAGACGT GTGAGCCGAG TGAACTGGAG
    ACAGAAATCA AACTGCCGAA GCTGCGGACC CTGGACGTGT TTTCCGGCTG TGGGGGATTG
    TCGGAAGGCT TCCACCAAGC AGGCATCTCG GAGACACTTT GGGCCATCGA GATGTGGGAC
    CCTGCGGCCC AGGCGTTCCG GCTCAACAAC CCTGGGTCCA CGGTGTTCAC AGAGGACTGC
    AACGTCCTGC TGAAGCTGGT CATGGCTGGG GAGGTGACCA ACTCCCGCGG CCAGAAGCTG
    CCTCAGAAGG GAGATGTGGA GATGCTGTGC GGCGGGCCGC CCTGCCAGGG CTTCAGCGGC
    ATGAACCGCT TCAACTCTCG AACCTACTCC AAATTCAAGA ACTCTCTGGT GGTCTCTTTC
    CTCAGCTACT GTGACTACTA CCGGCCCCGC TACTTCCTCT TGGAGAACGT TCGGAACTTC
    GTCTCCTTCA AGCGCTCCAT GGTCCTGAAG CTGACGCTGC GCTGCCTGGT CCGCATGGGC
    TACCAGTGCA CCTTTGGCGT GCTGCAGGCT GGTCAGTACG GCGTGGCCCA GACTCGGAGG
    CGAGCCATCA TCCTGGCTGC AGCCCCTGGG GAGCCACTCC CGCTGTTCCC GGAGCCGTTG
    CATGTGTTCG CACCCCGGGC CTGCCAGCTG AGCGTCGTAG TGGACGACAA GAAGTTTGTC
    AGCAACATCA CCAGGTTGAG CTCGGGTCCC TTCCGAACCA TCACCGTGCG GGACACCATG
    TCTGACCTCC CTGAGATCCG GAACGGGGCC TCGGCACTGG AGATTTCATA CAACGGGGAG
    CCCCAGTCCT GGTTCCAGAG GCAGCTCCGG GGCTCGCAGT ACCAGCCCAT CCTCAGGGAT
    CATATTTGCA AGGACATGAG CGCCTTGGTG GCTGCCCGCA TGCGGCACAT CCCCCTGGCC
    CCGGGCTCGG ACTGGCGTGA CCTGCCCAAC ATTGAGGTGC GGCTCTCTGA CGGCACCCTG
    GCCCGGAAGC TGCGGTACAA CTACCACGAC AAGAAGAACG GCTGCAGCAG CAGCGGCGCC
    CTCCGTGGGG TCTGCTCCTG TGTGGAAGGC AAGCCCTGTG AGCCTGCGGC CCGACAGTTT
    AACACCCTTA TCCCCTGGTG CCTGCCCCAC ACTGGGAACA GGCACAACCA CTGGGCCGGC
    CTCTACGGGC GTCTCGAGTG GGACGGCTTC TTCAGCACAA CTGTCACCAA CCCCGAGCCC
    ATGGGCAAGC AGGGCCGCGT GCTCCACCCC GAGCAGCACC GAGTGGTGAG CGTCCGGGAG
    TGCGCCCGCT CCCAGGGCTT CCCCGACACC TATCGGCTGT TCGGCAACAT CCTAGACAAG
    CACCGGCAGG TGGGTAATGC TGTGCCGCCG CCACTGGCCA AAGCCATCGG CTTGGAGATC
    AAGCGCTGCA TGTTGGCCAA AGCGCGCGAG AGCGCCTCAG GGCATATAAA AGTCATGTTT
    GGGGGATTCC TTGATGGCTT AGTGGTAAAG AATCTGCCTG CCAGTGCAGG AGACACGGAT
    TCAATCCCTG ATCCAGGAAG ATCCCACATG CTGCAGAGCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa44289
    Clone ID Related Accession (Same CDS sequence) NM_182651.2
    Accession Version NM_182651.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH376402.1, BC114063.1 and DV890836.1. On Jan 7, 2011 this sequence version replaced NM_182651.1. ##Evidence-Data-START## Transcript exon combination :: BC114063.1, AY244709.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGGAGGAG
    AAGAGACGCA GAACTACATA CAGAGAACTA ACCGAGAAGA AAATGACTCG AACCAAAATA
    GCCGTAGTGT CCAAGACCAA TCCTCCGAAG TGCACCGAGT GCTTGCAGTA CCTGGACGAC
    CCTGAGCTGA GATACGAGCA GCACCCCCCC GATGCGGTGG AAGAGATACA GATACTGACC
    AACGAGAGGT TGTCCATCTT TGATGCCAAC GAATCTGGCT TTGAGAGTTA CGAGGATTTG
    CCTCAGCACA AACTAACCTG CTTCAGCGTG TACTGTAAAC GCGGTCACCT TTGCCCGATC
    GACACCGGCC TCATTGAGAA GGATGTCGAG CTCCTCTTTT CTGGTTCAGC AAAGCCGATA
    TATGAGGATG ACCCATCTCC CGAAGGTGGT ATTAATGGCA AAAATTTTGG CCCCATAAAC
    GAATGGTGGA TTGCTGGTTT TGATGGAGGT GAAAAGGCTC TTCTTGGCTT TAGCACCTCA
    TTTGCCGAGT ATATCTTGAT GGATCCCAGC CCAGAGTACG CACCACTATT CAGCGTGATG
    CAGGAGAAGA TCTATATAAG TAAGATAGTG GTTGAGTTCC TGCAGAGCAA CCCTGACTCC
    ACCTACGAAG ACCTGATCAA TAAGATTGAG ACCACCGTTC CTCCTTGTAT GCTCAACTTG
    AATCGATTCA CAGAGGATTC TCTCCTGCGG CATGCCCAGT TCGTGGTGGA GCAAGTAGAG
    AGTTATGATC GGGCTGGGGA CAGTGACGAG CAGCCCATCT TCCTGAGCCC CTGCATGAGA
    GACCTCATCA AGCTGGCCGG GGTCACCCTG GGAAAAAGGC GAGCCGAGAG GCGGCAGACC
    ATCCGGCAAC CCGCCAAAGA GAAGGACAAG GGCCCCACCA AGGCCACCAC CACCAAGCTG
    GTCTACCAGA TCTTTGACAC TTTCTTTGCG GAGCAAATTG AAAAAGATGA CAAGGAAGAC
    AAGGAGAATG CCTTCAAGCG CCGGCGCTGT GGCGTCTGTG AGATTTGTCA ACAGCCCGAG
    TGTGGAAAGT GTAAGGCCTG TAAGGATATG GTTAAATTTG GTGGTAGCGG ACGGAGCAAG
    CAGGCTTGCC AAAAGAGGAG GTGTCCCAAC ATGGCCATGA AGGAGGCAGA CGATGACGAG
    GAAGTGGATG ACAATATTCC AGAGATGCCA TCACCCAAAA AGATGCATCA GGGGAAGAAA
    AAGAAGCAGA ATAAGAATCG GATCTCTTGG GTTGGCGATG CCGTCAAGAC TGACGGGAAG
    AAGAGTTACT ACAAGAAGGT ATGCATCGAC TCGGAAACCC TGGAAGTGGG GGACTGTGTT
    TCTGTAATTC CAGACGACTC TTCAAAACCA CTGTATCTAG CAAGGGTCAC GGCGCTGTGG
    GAGGACAGCA GCAATGGGCA GATGTTCCAT GCCCACTGGT TCTGTGCTGG GACGGACACG
    GTCCTCGGGG CCACATCGGA CCCCCTGGAG CTGTTCCTGG TTGACGAGTG TGAGGACATG
    CAGCTCTCGT ACATCCACAG CAAGGTGCAG GTCATTTATA AGGCGCCCTC AGAGAACTGG
    GCCATGGAGG GAGGCGTGGA CCCCGAGGCC CTGATGTCAG AGGACGACGG GAAGACCTAC
    TTCTACCAGC TGTGGTACGA CCAAGACTAC GCGAGATTTG AGTCCCCTCC GAAAACTCAG
    CCGACGGAGG ACAACAAGTA CAAGTTCTGC GCAAGCTGTG CACGTCTGGC CGAAATGAGG
    CAGAAGGAAA TCCCCAGGGT CGTGGAGCAG CTCCAGGACC TGGAAGGCCG CGTCCTCTAC
    AGCCTCGCCA CCAAGAACGG CGTCCAGTAC CGGGTGGGCG ATGGCGTGTA CCTCCCTCCC
    GAGGCCTTCA CCTTCAACAT CAAGCTGTCC AGTCCTGTGA AACGCCCCCG GAAGGAGCCT
    GTGGACGAAG CTCTGTATCC AGAACACTAC CGGAAGTACT CTGACTACAT CAAGGGCAGC
    AACCTGGATG CCCCTGAGCC CTACCGTATT GGCCGCATAA AGGAGATCTT CTGCAGCAAG
    AAGAGCAACG GCCGGCCCAA TGAGACAGAC ATCAAGATCA GGGTCAACAA GTTCTACAGG
    CCGGAGAACA CACACAAGTC TACCCCAGCC AGTTACCACG CAGACATCAA CCTGCTTTAC
    TGGAGCGATG AGGAGGCCGT GGTGGACTTC AAGGCCGTGC AGGGCCGCTG CACCGTGGAG
    TACGGAGAGG ACCTGCCTCA GTGCCTCCAG GACTTCTCCG CTGGTGGCCC CGATCGCTTC
    TATTTTCTCG AGGCCTATAA CGCCAAGAGC AAAAGCTTTG AAGATCCTCC GAACCACGCC
    CGGAGCACCG GAAATAAAGG GAAAGGGAAG GGGAAAGGAA AAAACAGGAC GAAATCTCAG
    ACGTGTGAGC CGAGTGAACT GGAGACAGAA ATCAAACTGC CGAAGCTGCG GACCCTGGAC
    GTGTTTTCCG GCTGTGGGGG ATTGTCGGAA GGCTTCCACC AAGCAGGCAT CTCGGAGACA
    CTTTGGGCCA TCGAGATGTG GGACCCTGCG GCCCAGGCGT TCCGGCTCAA CAACCCTGGG
    TCCACGGTGT TCACAGAGGA CTGCAACGTC CTGCTGAAGC TGGTCATGGC TGGGGAGGTG
    ACCAACTCCC GCGGCCAGAA GCTGCCTCAG AAGGGAGATG TGGAGATGCT GTGCGGCGGG
    CCGCCCTGCC AGGGCTTCAG CGGCATGAAC CGCTTCAACT CTCGAACCTA CTCCAAATTC
    AAGAACTCTC TGGTGGTCTC TTTCCTCAGC TACTGTGACT ACTACCGGCC CCGCTACTTC
    CTCTTGGAGA ACGTTCGGAA CTTCGTCTCC TTCAAGCGCT CCATGGTCCT GAAGCTGACG
    CTGCGCTGCC TGGTCCGCAT GGGCTACCAG TGCACCTTTG GCGTGCTGCA GGCTGGTCAG
    TACGGCGTGG CCCAGACTCG GAGGCGAGCC ATCATCCTGG CTGCAGCCCC TGGGGAGCCA
    CTCCCGCTGT TCCCGGAGCC GTTGCATGTG TTCGCACCCC GGGCCTGCCA GCTGAGCGTC
    GTAGTGGACG ACAAGAAGTT TGTCAGCAAC ATCACCAGGT TGAGCTCGGG TCCCTTCCGA
    ACCATCACCG TGCGGGACAC CATGTCTGAC CTCCCTGAGA TCCGGAACGG GGCCTCGGCA
    CTGGAGATTT CATACAACGG GGAGCCCCAG TCCTGGTTCC AGAGGCAGCT CCGGGGCTCG
    CAGTACCAGC CCATCCTCAG GGATCATATT TGCAAGGACA TGAGCGCCTT GGTGGCTGCC
    CGCATGCGGC ACATCCCCCT GGCCCCGGGC TCGGACTGGC GTGACCTGCC CAACATTGAG
    GTGCGGCTCT CTGACGGCAC CCTGGCCCGG AAGCTGCGGT ACAACTACCA CGACAAGAAG
    AACGGCTGCA GCAGCAGCGG CGCCCTCCGT GGGGTCTGCT CCTGTGTGGA AGGCAAGCCC
    TGTGAGCCTG CGGCCCGACA GTTTAACACC CTTATCCCCT GGTGCCTGCC CCACACTGGG
    AACAGGCACA ACCACTGGGC CGGCCTCTAC GGGCGTCTCG AGTGGGACGG CTTCTTCAGC
    ACAACTGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCCGAGCAG
    CACCGAGTGG TGAGCGTCCG GGAGTGCGCC CGCTCCCAGG GCTTCCCCGA CACCTATCGG
    CTGTTCGGCA ACATCCTAGA CAAGCACCGG CAGGTGGGTA ATGCTGTGCC GCCGCCACTG
    GCCAAAGCCA TCGGCTTGGA GATCAAGCGC TGCATGTTGG CCAAAGCGCG CGAGAGCGCC
    TCAGCTAAAA TCAAGGAGGA GGCTGCCAAG GACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_872592.2
    CDS24..4859
    Translation

    Target ORF information:

    RefSeq Version NM_182651.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA methyltransferase 1 (DNMT1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_182651.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGGAGGAG
    AAGAGACGCA GAACTACATA CAGAGAACTA ACCGAGAAGA AAATGACTCG AACCAAAATA
    GCCGTAGTGT CCAAGACCAA TCCTCCGAAG TGCACCGAGT GCTTGCAGTA CCTGGACGAC
    CCTGAGCTGA GATACGAGCA GCACCCCCCC GATGCGGTGG AAGAGATACA GATACTGACC
    AACGAGAGGT TGTCCATCTT TGATGCCAAC GAATCTGGCT TTGAGAGTTA CGAGGATTTG
    CCTCAGCACA AACTAACCTG CTTCAGCGTG TACTGTAAAC GCGGTCACCT TTGCCCGATC
    GACACCGGCC TCATTGAGAA GGATGTCGAG CTCCTCTTTT CTGGTTCAGC AAAGCCGATA
    TATGAGGATG ACCCATCTCC CGAAGGTGGT ATTAATGGCA AAAATTTTGG CCCCATAAAC
    GAATGGTGGA TTGCTGGTTT TGATGGAGGT GAAAAGGCTC TTCTTGGCTT TAGCACCTCA
    TTTGCCGAGT ATATCTTGAT GGATCCCAGC CCAGAGTACG CACCACTATT CAGCGTGATG
    CAGGAGAAGA TCTATATAAG TAAGATAGTG GTTGAGTTCC TGCAGAGCAA CCCTGACTCC
    ACCTACGAAG ACCTGATCAA TAAGATTGAG ACCACCGTTC CTCCTTGTAT GCTCAACTTG
    AATCGATTCA CAGAGGATTC TCTCCTGCGG CATGCCCAGT TCGTGGTGGA GCAAGTAGAG
    AGTTATGATC GGGCTGGGGA CAGTGACGAG CAGCCCATCT TCCTGAGCCC CTGCATGAGA
    GACCTCATCA AGCTGGCCGG GGTCACCCTG GGAAAAAGGC GAGCCGAGAG GCGGCAGACC
    ATCCGGCAAC CCGCCAAAGA GAAGGACAAG GGCCCCACCA AGGCCACCAC CACCAAGCTG
    GTCTACCAGA TCTTTGACAC TTTCTTTGCG GAGCAAATTG AAAAAGATGA CAAGGAAGAC
    AAGGAGAATG CCTTCAAGCG CCGGCGCTGT GGCGTCTGTG AGATTTGTCA ACAGCCCGAG
    TGTGGAAAGT GTAAGGCCTG TAAGGATATG GTTAAATTTG GTGGTAGCGG ACGGAGCAAG
    CAGGCTTGCC AAAAGAGGAG GTGTCCCAAC ATGGCCATGA AGGAGGCAGA CGATGACGAG
    GAAGTGGATG ACAATATTCC AGAGATGCCA TCACCCAAAA AGATGCATCA GGGGAAGAAA
    AAGAAGCAGA ATAAGAATCG GATCTCTTGG GTTGGCGATG CCGTCAAGAC TGACGGGAAG
    AAGAGTTACT ACAAGAAGGT ATGCATCGAC TCGGAAACCC TGGAAGTGGG GGACTGTGTT
    TCTGTAATTC CAGACGACTC TTCAAAACCA CTGTATCTAG CAAGGGTCAC GGCGCTGTGG
    GAGGACAGCA GCAATGGGCA GATGTTCCAT GCCCACTGGT TCTGTGCTGG GACGGACACG
    GTCCTCGGGG CCACATCGGA CCCCCTGGAG CTGTTCCTGG TTGACGAGTG TGAGGACATG
    CAGCTCTCGT ACATCCACAG CAAGGTGCAG GTCATTTATA AGGCGCCCTC AGAGAACTGG
    GCCATGGAGG GAGGCGTGGA CCCCGAGGCC CTGATGTCAG AGGACGACGG GAAGACCTAC
    TTCTACCAGC TGTGGTACGA CCAAGACTAC GCGAGATTTG AGTCCCCTCC GAAAACTCAG
    CCGACGGAGG ACAACAAGTA CAAGTTCTGC GCAAGCTGTG CACGTCTGGC CGAAATGAGG
    CAGAAGGAAA TCCCCAGGGT CGTGGAGCAG CTCCAGGACC TGGAAGGCCG CGTCCTCTAC
    AGCCTCGCCA CCAAGAACGG CGTCCAGTAC CGGGTGGGCG ATGGCGTGTA CCTCCCTCCC
    GAGGCCTTCA CCTTCAACAT CAAGCTGTCC AGTCCTGTGA AACGCCCCCG GAAGGAGCCT
    GTGGACGAAG CTCTGTATCC AGAACACTAC CGGAAGTACT CTGACTACAT CAAGGGCAGC
    AACCTGGATG CCCCTGAGCC CTACCGTATT GGCCGCATAA AGGAGATCTT CTGCAGCAAG
    AAGAGCAACG GCCGGCCCAA TGAGACAGAC ATCAAGATCA GGGTCAACAA GTTCTACAGG
    CCGGAGAACA CACACAAGTC TACCCCAGCC AGTTACCACG CAGACATCAA CCTGCTTTAC
    TGGAGCGATG AGGAGGCCGT GGTGGACTTC AAGGCCGTGC AGGGCCGCTG CACCGTGGAG
    TACGGAGAGG ACCTGCCTCA GTGCCTCCAG GACTTCTCCG CTGGTGGCCC CGATCGCTTC
    TATTTTCTCG AGGCCTATAA CGCCAAGAGC AAAAGCTTTG AAGATCCTCC GAACCACGCC
    CGGAGCACCG GAAATAAAGG GAAAGGGAAG GGGAAAGGAA AAAACAGGAC GAAATCTCAG
    ACGTGTGAGC CGAGTGAACT GGAGACAGAA ATCAAACTGC CGAAGCTGCG GACCCTGGAC
    GTGTTTTCCG GCTGTGGGGG ATTGTCGGAA GGCTTCCACC AAGCAGGCAT CTCGGAGACA
    CTTTGGGCCA TCGAGATGTG GGACCCTGCG GCCCAGGCGT TCCGGCTCAA CAACCCTGGG
    TCCACGGTGT TCACAGAGGA CTGCAACGTC CTGCTGAAGC TGGTCATGGC TGGGGAGGTG
    ACCAACTCCC GCGGCCAGAA GCTGCCTCAG AAGGGAGATG TGGAGATGCT GTGCGGCGGG
    CCGCCCTGCC AGGGCTTCAG CGGCATGAAC CGCTTCAACT CTCGAACCTA CTCCAAATTC
    AAGAACTCTC TGGTGGTCTC TTTCCTCAGC TACTGTGACT ACTACCGGCC CCGCTACTTC
    CTCTTGGAGA ACGTTCGGAA CTTCGTCTCC TTCAAGCGCT CCATGGTCCT GAAGCTGACG
    CTGCGCTGCC TGGTCCGCAT GGGCTACCAG TGCACCTTTG GCGTGCTGCA GGCTGGTCAG
    TACGGCGTGG CCCAGACTCG GAGGCGAGCC ATCATCCTGG CTGCAGCCCC TGGGGAGCCA
    CTCCCGCTGT TCCCGGAGCC GTTGCATGTG TTCGCACCCC GGGCCTGCCA GCTGAGCGTC
    GTAGTGGACG ACAAGAAGTT TGTCAGCAAC ATCACCAGGT TGAGCTCGGG TCCCTTCCGA
    ACCATCACCG TGCGGGACAC CATGTCTGAC CTCCCTGAGA TCCGGAACGG GGCCTCGGCA
    CTGGAGATTT CATACAACGG GGAGCCCCAG TCCTGGTTCC AGAGGCAGCT CCGGGGCTCG
    CAGTACCAGC CCATCCTCAG GGATCATATT TGCAAGGACA TGAGCGCCTT GGTGGCTGCC
    CGCATGCGGC ACATCCCCCT GGCCCCGGGC TCGGACTGGC GTGACCTGCC CAACATTGAG
    GTGCGGCTCT CTGACGGCAC CCTGGCCCGG AAGCTGCGGT ACAACTACCA CGACAAGAAG
    AACGGCTGCA GCAGCAGCGG CGCCCTCCGT GGGGTCTGCT CCTGTGTGGA AGGCAAGCCC
    TGTGAGCCTG CGGCCCGACA GTTTAACACC CTTATCCCCT GGTGCCTGCC CCACACTGGG
    AACAGGCACA ACCACTGGGC CGGCCTCTAC GGGCGTCTCG AGTGGGACGG CTTCTTCAGC
    ACAACTGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCCGAGCAG
    CACCGAGTGG TGAGCGTCCG GGAGTGCGCC CGCTCCCAGG GCTTCCCCGA CACCTATCGG
    CTGTTCGGCA ACATCCTAGA CAAGCACCGG CAGGTGGGTA ATGCTGTGCC GCCGCCACTG
    GCCAAAGCCA TCGGCTTGGA GATCAAGCGC TGCATGTTGG CCAAAGCGCG CGAGAGCGCC
    TCAGCTAAAA TCAAGGAGGA GGCTGCCAAG GACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45072
    Clone ID Related Accession (Same CDS sequence) XM_005208712.3 , XM_005208712.4
    Accession Version XM_005208712.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208712.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGCTAAAAT CAAGGAGGAG
    GCTGCCAAGG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208769.2
    CDS75..4949
    Translation

    Target ORF information:

    RefSeq Version XM_005208712.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208712.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGCTAAAAT CAAGGAGGAG
    GCTGCCAAGG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45072
    Clone ID Related Accession (Same CDS sequence) XM_005208712.3 , XM_005208712.4
    Accession Version XM_005208712.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208712.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGCTAAAAT CAAGGAGGAG
    GCTGCCAAGG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208769.2
    CDS79..4953
    Translation

    Target ORF information:

    RefSeq Version XM_005208712.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208712.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGCTAAAAT CAAGGAGGAG
    GCTGCCAAGG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45073
    Clone ID Related Accession (Same CDS sequence) XM_010806776.2 , XM_010806776.3
    Accession Version XM_010806776.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805078.1
    CDS75..4922
    Translation

    Target ORF information:

    RefSeq Version XM_010806776.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806776.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45073
    Clone ID Related Accession (Same CDS sequence) XM_010806776.2 , XM_010806776.3
    Accession Version XM_010806776.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA (cytosine-5)-methyltransferase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806776.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805078.1
    CDS79..4926
    Translation

    Target ORF information:

    RefSeq Version XM_010806776.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA methyltransferase 1 (DNMT1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806776.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTGCCC GAACCGCCCC GGCGCGGGTG CCTGCGCTGG CCTCCCGGGC CTTCTCACTG 
    CCTGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ATAGTTTGAC AGAAAAGGAA
    TGTGTGAAGG AGAAACTGAA TCTCTTGCAC GAATTTCTGC GGACAGAAAT AAAGAATCAG
    TTATGTGATT TGGAAACCAA ATTGCATAAA GAAGAATTAT CTGAGGAGGG CTACCTGGCT
    AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGAGCTCA TGCTTTCAGT
    CGGGAAGCGA ATGGATGTCT AGAGAACGGG AGCCAGACAA GTGGTGAGGA TTGCAGAGTG
    GTAATGGCAG AGAAAGGCAA GCCCCCCAAA CCTGTCTCCA GACTTTACAC GCCCAGGAGA
    AGCAAGTCTG ATGGAGAAAC AAAGTCTGAA GTCTCTTCTA GCCCCAGGAT TACAAGGAAG
    ACTACCAGGC AGACCACCAT CACATCTCAT TTCCCACGGG GCCCTGCCAA ACGAAAACCT
    GAGGAAGAAC CTGAAAAAGT GAAGTCAGAC GATTCTGTTG ATGAAGAAAA AGACCAGGAG
    GAAAAGAGAC GTCGAGTTAC ATCCAGAGAA CGAGTTGCTG GGCTGCTCCC TGCAGAAGAA
    CCAGGAAGAG TAAGACCAGG AACACACATG GAAGAAGAAG GAAGAGATGA TAAAGAAGAA
    AAGAGACTCA GAAGTCAAAC CAAAGAACCG ACACCTAAAC ACAAAGCTAA GGAGGAGCCA
    GACAGAGATG TGAGGCCTGG AGGAGCTCAG GCTGAAATGA ATGAAGGAGA AGACAAAGAT
    GAAAAGAGGC ACAGAAGTCA ACCCAAAGAT CTAGCTAGCA AACGGAGACC AGAAGAAAAA
    GAACCTGAAA GAGTAAAGCC ACAAGTTTCT GATGAGAAAG ATGAAGATGA AAAGTTCTGG
    AGAATACAGT TCACATATCA AAGTACCAGC AGGGAGGAGA AGAGACGCAG AACTACATAC
    AGAGAACTAA CCGAGAAGAA AATGACTCGA ACCAAAATAG CCGTAGTGTC CAAGACCAAT
    CCTCCGAAGT GCACCGAGTG CTTGCAGTAC CTGGACGACC CTGAGCTGAG ATACGAGCAG
    CACCCCCCCG ATGCGGTGGA AGAGATACAG ATACTGACCA ACGAGAGGTT GTCCATCTTT
    GATGCCAACG AATCTGGCTT TGAGAGTTAC GAGGATTTGC CTCAGCACAA ACTAACCTGC
    TTCAGCGTGT ACTGTAAACG CGGTCACCTT TGCCCGATCG ACACCGGCCT CATTGAGAAG
    GATGTCGAGC TCCTCTTTTC TGGTTCAGCA AAGCCGATAT ATGAGGATGA CCCATCTCCC
    GAAGGTGGTA TTAATGGCAA AAATTTTGGC CCCATAAACG AATGGTGGAT TGCTGGTTTT
    GATGGAGGTG AAAAGGCTCT TCTTGGCTTT AGCACCTCAT TTGCCGAGTA TATCTTGATG
    GATCCCAGCC CAGAGTACGC ACCACTATTC AGCGTGATGC AGGAGAAGAT CTATATAAGT
    AAGATAGTGG TTGAGTTCCT GCAGAGCAAC CCTGACTCCA CCTACGAAGA CCTGATCAAT
    AAGATTGAGA CCACCGTTCC TCCTTGTATG CTCAACTTGA ATCGATTCAC AGAGGATTCT
    CTCCTGCGGC ATGCCCAGTT CGTGGTGGAG CAAGTAGAGA GTTATGATCG GGCTGGGGAC
    AGTGACGAGC AGCCCATCTT CCTGAGCCCC TGCATGAGAG ACCTCATCAA GCTGGCCGGG
    GTCACCCTGG GAAAAAGGCG AGCCGAGAGG CGGCAGACCA TCCGGCAACC CGCCAAAGAG
    AAGGACAAGG GCCCCACCAA GGCCACCACC ACCAAGCTGG TCTACCAGAT CTTTGACACT
    TTCTTTGCGG AGCAAATTGA AAAAGATGAC AAGGAAGACA AGGAGAATGC CTTCAAGCGC
    CGGCGCTGTG GCGTCTGTGA GATTTGTCAA CAGCCCGAGT GTGGAAAGTG TAAGGCCTGT
    AAGGATATGG TTAAATTTGG TGGTAGCGGA CGGAGCAAGC AGGCTTGCCA AAAGAGGAGG
    TGTCCCAACA TGGCCATGAA GGAGGCAGAC GATGACGAGG AAGTGGATGA CAATATTCCA
    GAGATGCCAT CACCCAAAAA GATGCATCAG GGGAAGAAAA AGAAGCAGAA TAAGAATCGG
    ATCTCTTGGG TTGGCGATGC CGTCAAGACT GACGGGAAGA AGAGTTACTA CAAGAAGGTA
    TGCATCGACT CGGAAACCCT GGAAGTGGGG GACTGTGTTT CTGTAATTCC AGACGACTCT
    TCAAAACCAC TGTATCTAGC AAGGGTCACG GCGCTGTGGG AGGACAGCAG CAATGGGCAG
    ATGTTCCATG CCCACTGGTT CTGTGCTGGG ACGGACACGG TCCTCGGGGC CACATCGGAC
    CCCCTGGAGC TGTTCCTGGT TGACGAGTGT GAGGACATGC AGCTCTCGTA CATCCACAGC
    AAGGTGCAGG TCATTTATAA GGCGCCCTCA GAGAACTGGG CCATGGAGGG AGGCGTGGAC
    CCCGAGGCCC TGATGTCAGA GGACGACGGG AAGACCTACT TCTACCAGCT GTGGTACGAC
    CAAGACTACG CGAGATTTGA GTCCCCTCCG AAAACTCAGC CGACGGAGGA CAACAAGTAC
    AAGTTCTGCG CAAGCTGTGC ACGTCTGGCC GAAATGAGGC AGAAGGAAAT CCCCAGGGTC
    GTGGAGCAGC TCCAGGACCT GGAAGGCCGC GTCCTCTACA GCCTCGCCAC CAAGAACGGC
    GTCCAGTACC GGGTGGGCGA TGGCGTGTAC CTCCCTCCCG AGGCCTTCAC CTTCAACATC
    AAGCTGTCCA GTCCTGTGAA ACGCCCCCGG AAGGAGCCTG TGGACGAAGC TCTGTATCCA
    GAACACTACC GGAAGTACTC TGACTACATC AAGGGCAGCA ACCTGGATGC CCCTGAGCCC
    TACCGTATTG GCCGCATAAA GGAGATCTTC TGCAGCAAGA AGAGCAACGG CCGGCCCAAT
    GAGACAGACA TCAAGATCAG GGTCAACAAG TTCTACAGGC CGGAGAACAC ACACAAGTCT
    ACCCCAGCCA GTTACCACGC AGACATCAAC CTGCTTTACT GGAGCGATGA GGAGGCCGTG
    GTGGACTTCA AGGCCGTGCA GGGCCGCTGC ACCGTGGAGT ACGGAGAGGA CCTGCCTCAG
    TGCCTCCAGG ACTTCTCCGC TGGTGGCCCC GATCGCTTCT ATTTTCTCGA GGCCTATAAC
    GCCAAGAGCA AAAGCTTTGA AGATCCTCCG AACCACGCCC GGAGCACCGG AAATAAAGGG
    AAAGGGAAGG GGAAAGGAAA AAACAGGACG AAATCTCAGA CGTGTGAGCC GAGTGAACTG
    GAGACAGAAA TCAAACTGCC GAAGCTGCGG ACCCTGGACG TGTTTTCCGG CTGTGGGGGA
    TTGTCGGAAG GCTTCCACCA AGCAGGCATC TCGGAGACAC TTTGGGCCAT CGAGATGTGG
    GACCCTGCGG CCCAGGCGTT CCGGCTCAAC AACCCTGGGT CCACGGTGTT CACAGAGGAC
    TGCAACGTCC TGCTGAAGCT GGTCATGGCT GGGGAGGTGA CCAACTCCCG CGGCCAGAAG
    CTGCCTCAGA AGGGAGATGT GGAGATGCTG TGCGGCGGGC CGCCCTGCCA GGGCTTCAGC
    GGCATGAACC GCTTCAACTC TCGAACCTAC TCCAAATTCA AGAACTCTCT GGTGGTCTCT
    TTCCTCAGCT ACTGTGACTA CTACCGGCCC CGCTACTTCC TCTTGGAGAA CGTTCGGAAC
    TTCGTCTCCT TCAAGCGCTC CATGGTCCTG AAGCTGACGC TGCGCTGCCT GGTCCGCATG
    GGCTACCAGT GCACCTTTGG CGTGCTGCAG GCTGGTCAGT ACGGCGTGGC CCAGACTCGG
    AGGCGAGCCA TCATCCTGGC TGCAGCCCCT GGGGAGCCAC TCCCGCTGTT CCCGGAGCCG
    TTGCATGTGT TCGCACCCCG GGCCTGCCAG CTGAGCGTCG TAGTGGACGA CAAGAAGTTT
    GTCAGCAACA TCACCAGGTT GAGCTCGGGT CCCTTCCGAA CCATCACCGT GCGGGACACC
    ATGTCTGACC TCCCTGAGAT CCGGAACGGG GCCTCGGCAC TGGAGATTTC ATACAACGGG
    GAGCCCCAGT CCTGGTTCCA GAGGCAGCTC CGGGGCTCGC AGTACCAGCC CATCCTCAGG
    GATCATATTT GCAAGGACAT GAGCGCCTTG GTGGCTGCCC GCATGCGGCA CATCCCCCTG
    GCCCCGGGCT CGGACTGGCG TGACCTGCCC AACATTGAGG TGCGGCTCTC TGACGGCACC
    CTGGCCCGGA AGCTGCGGTA CAACTACCAC GACAAGAAGA ACGGCTGCAG CAGCAGCGGC
    GCCCTCCGTG GGGTCTGCTC CTGTGTGGAA GGCAAGCCCT GTGAGCCTGC GGCCCGACAG
    TTTAACACCC TTATCCCCTG GTGCCTGCCC CACACTGGGA ACAGGCACAA CCACTGGGCC
    GGCCTCTACG GGCGTCTCGA GTGGGACGGC TTCTTCAGCA CAACTGTCAC CAACCCCGAG
    CCCATGGGCA AGCAGGGCCG CGTGCTCCAC CCCGAGCAGC ACCGAGTGGT GAGCGTCCGG
    GAGTGCGCCC GCTCCCAGGG CTTCCCCGAC ACCTATCGGC TGTTCGGCAA CATCCTAGAC
    AAGCACCGGC AGGTGGGTAA TGCTGTGCCG CCGCCACTGG CCAAAGCCAT CGGCTTGGAG
    ATCAAGCGCT GCATGTTGGC CAAAGCGCGC GAGAGCGCCT CAGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.