Crkl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Crkl
Entrez Gene ID	12929
Full Name	v-crk avian sarcoma virus CT10 oncogene homolog-like
Synonyms	1110025F07Rik,AA589403,AI325100,Crkol
General protein information	Preferred Names v-crk avian sarcoma virus CT10 oncogene homolog-like Names crk-like protein v-crk sarcoma virus CT10 oncogene homolog-like
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 A3
Summary	This gene is part of a family of adapter proteins that mediate formation of signal transduction complexes in response to extracellular stimuli, such as growth and differentiation factors. Protein-protein interactions occur through the SH2 domain, which binds phosphorylated tyrosine residues, and the SH3 domain, which binds proline-rich peptide motifs. These interactions promote recruitment and activation of effector proteins to regulate cell migration, adhesion, and proliferation. In certain mouse genetic backgrounds this protein is essential for embryonic development. It is important for neural crest cell differentiation and survival and is proposed to play an important role in transducing the oncogenic signal of Bcr/Abl. Deletion of this gene in mouse mimics the phenotype of DiGeorge/velocardiofacial syndrome in human. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Mar 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007764.5	NP_031790.2	crk-like protein isoform 1
NM_001277231.1	NP_001264160.1	crk-like protein isoform 2

Pathways from BioSystems

Homologies

	CRKL	XP_001086123.1
Gallus gallus (chicken)	CRKL	XP_415233.1
Pan troglodytes (chimpanzee)	CRKL	XP_525530.2
Canis lupus familiaris (dog)	CRKL	XP_849949.1
Xenopus tropicalis (tropical clawed frog)	crkl	NP_989060.1
Mus musculus (house mouse)	Crkl	NP_031790.2
Homo sapiens (human)	CRKL	NP_005198.1
Rattus norvegicus (Norway rat)	Crkl	NP_001008285.1
Rattus norvegicus (Norway rat)	LOC100911248	XP_003751143.1
Danio rerio (zebrafish)	crkl	NP_998703.1

Gene Ontology
Bibliography

Related articles in PubMed

Crk proteins transduce FGF signaling to promote lens fiber cell elongation.
Collins TN, Mao Y, Li H, Bouaziz M, Hong A, Feng GS, Wang F, Quilliam LA, Chen L, Park T, Curran T, Zhang X
eLife7(2018 Jan)

Murine model indicates 22q11.2 signaling adaptor
Haller M, Mo Q, Imamoto A, Lamb DJ
Proceedings of the National Academy of Sciences of the United States of America114(19)4981-4986(2017 May)

Genetic Drivers of Kidney Defects in the DiGeorge Syndrome.
Lopez-Rivera E, Liu YP, Verbitsky M, Anderson BR, Capone VP, Otto EA, Yan Z, Mitrotti A, Martino J, Steers NJ, Fasel DA, Vukojevic K, Deng R, Racedo SE, Liu Q, Werth M, Westland R, Vivante A, Makar GS, Bodria M, Sampson MG, Gillies CE, Vega-Warner V, Maiorana M, Petrey DS, Honig B, Lozanovski VJ, Salomon R, Heidet L, Carpentier W, Gaillard D, Carrea A, Gesualdo L, Cusi D, Izzi C, Scolari F, van Wijk JA, Arapovic A, Saraga-Babic M, Saraga M, Kunac N, Samii A, McDonald-McGinn DM, Crowley TB, Zackai EH, Drozdz D, Miklaszewska M, Tkaczyk M, Sikora P, Szczepanska M, Mizerska-Wasiak M, Krzemien G, Szmigielska A, Zaniew M, Darlow JM, Puri P, Barton D, Casolari E, Furth SL, Warady BA, Gucev Z, Hakonarson H, Flogelova H, Tasic V, Latos-Bielenska A, Materna-Kiryluk A, Allegri L, Wong CS, Drummond IA, D'Agati V, Imamoto A, Barasch JM, Hildebrandt F, Kiryluk K, Lifton RP, Morrow BE, Jeanpierre C, Papaioannou VE, Ghiggeri GM, Gharavi AG, Katsanis N, Sanna-Cherchi S
The New England journal of medicine376(8)742-754(2017 02)

BCR/ABL P190 transgenic mice develop leukemia in the absence of Crkl.
Hemmeryckx B, Reichert A, Watanabe M, Kaartinen V, de Jong R, Pattengale PK, Groffen J, Heisterkamp N
Oncogene21(20)3225-31(2002 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest that Crk and CrkL play essential overlapping roles in fibroblast growth.
Title: Fibroblast Growth Requires CT10 Regulator of Kinase (Crk) and Crk-like (CrkL).

The study screened CrkL binding proteins using RNA interference (RNAi) and identified Sorbs1 and Sorbs2 as two proteins that are enriched at AChR clusters and are required for the formation of AChR aggregation in vitro.
Title: Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation.

CRKL plays an important role in hepatocarcinoma cell proliferation, invasion and migration as well hepatocarcinoma malignancy and metastasis.
Title: CRKL knockdown promotes in vitro proliferation, migration and invasion, in vivo tumor malignancy and lymph node metastasis of murine hepatocarcinoma Hca-P cells.

Differential migration of CRK/CRKL-deficient T cells resulted in efficient graft-versus-leukemia responses with minimal graft-versus-host disease in mice.
Title: CRK proteins selectively regulate T cell migration into inflamed tissues.

CRKL is shown to act as a potential suppressor and to provide new insight for both the malignant behaviors of hepatocarcinoma cells and lymphatic metastasis mechanism of hepatocarcinoma.
Title: CRKL overexpression suppresses in vitro proliferation, invasion and migration of murine hepatocarcinoma Hca-P cells.

The following Crkl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Crkl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07884	NM_001277231.1 Latest version!	Mus musculus v-crk avian sarcoma virus CT10 oncogene homolog-like (Crkl), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu00758	NM_007764.5 Latest version!	Mus musculus v-crk avian sarcoma virus CT10 oncogene homolog-like (Crkl), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Crkl ( NM_001277231.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001264160.1 Mus musculus v-crk avian sarcoma virus CT10 oncogene homolog-like (Crkl), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07884
Clone ID Related Accession (Same CDS sequence)	NM_001277231.1
Accession Version	NM_001277231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	crk-like protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK052315.1, CA328740.1, AK048641.1, AW541802.2, BF453354.1, BF464821.1 and BY471344.1. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift and an early stop codon compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660825.62902.1, SRR7652917.394701.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTCCG CCAGGTTTGA TTCTTCAGAC CGCTCTGCCT GGTACATGGG GCCAGTGACT 
CGCCAGGAGG CGCAGACTCG TCTCCAAGGC CAGCGCCATG GCATGTTCCT AGTCCGGGAC 
TCATCTACCT GCCCCGGGGA CTATGTACTG TCCGTGTCCG AGAACTCGCG TGTCTCGCAC 
TACATCATCA ACTCCCTGCC CAACCGCCGC TTTAAGATCG GGGACCAGGA GTTTGACCAT 
TTGCCGGCCT TGTTAGAGTT CTACAAGATC CACTACCTGG ACACTACCAC CTTAATCGAA 
CCGGCGCCCA GGTTGGTGAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264160.1
CDS	494..820
Translation	MSSARFDSSDRSAWYMGPVTRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRLVTL

Target ORF information:

RefSeq Version	NM_001277231.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus v-crk avian sarcoma virus CT10 oncogene homolog-like (Crkl), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277231.1

ORF Insert Sequence:

ATGTCCTCCG CCAGGTTTGA TTCTTCAGAC CGCTCTGCCT GGTACATGGG GCCAGTGACT 
CGCCAGGAGG CGCAGACTCG TCTCCAAGGC CAGCGCCATG GCATGTTCCT AGTCCGGGAC 
TCATCTACCT GCCCCGGGGA CTATGTACTG TCCGTGTCCG AGAACTCGCG TGTCTCGCAC 
TACATCATCA ACTCCCTGCC CAACCGCCGC TTTAAGATCG GGGACCAGGA GTTTGACCAT 
TTGCCGGCCT TGTTAGAGTT CTACAAGATC CACTACCTGG ACACTACCAC CTTAATCGAA 
CCGGCGCCCA GGTTGGTGAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crkl ( NM_007764.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031790.2 Mus musculus v-crk avian sarcoma virus CT10 oncogene homolog-like (Crkl), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00758
Clone ID Related Accession (Same CDS sequence)	NM_007764.5
Accession Version	NM_007764.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	crk-like protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK052315.1, AK048641.1, AW541802.2, BF453354.1, BF464821.1 and BY471344.1. On Mar 23, 2013 this sequence version replaced NM_007764.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK052315.1, SRR1660815.35856.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTCCG CCAGGTTTGA TTCTTCAGAC CGCTCTGCCT GGTACATGGG GCCAGTGACT 
CGCCAGGAGG CGCAGACTCG TCTCCAAGGC CAGCGCCATG GCATGTTCCT AGTCCGGGAC 
TCATCTACCT GCCCCGGGGA CTATGTACTG TCCGTGTCCG AGAACTCGCG TGTCTCGCAC 
TACATCATCA ACTCCCTGCC CAACCGCCGC TTTAAGATCG GGGACCAGGA GTTTGACCAT 
TTGCCGGCCT TGTTAGAGTT CTACAAGATC CACTACCTGG ACACTACCAC CTTAATCGAA 
CCGGCGCCCA GGTACCCAAG CCCACCAGTG GGTTCTGTCT CAGCACCCAA CTTACCTACA 
GCAGAAGAAA ATCTGGAATA TGTACGGACC CTTTATGATT TTCCTGGGAA TGATGCTGAA 
GACCTACCCT TTAAAAAGGG CGAGCTTCTA GTGATAATAG AAAAGCCTGA AGAGCAGTGG 
TGGAGTGCCC GCAACAAGGA CGGCCGGGTT GGGATGATTC CTGTCCCTTA CGTTGAAAAG 
CTTGTGAGGT CTTCGCCACA TGGAAAGCAT GGAAATAGGA ATTCTAACAG TTATGGCATC 
CCAGAACCTG CTCATGCGTA TGCTCAACCT CAGACCACAA CTCCTCTACC TACAGTTGCC 
AGTACTCCTG GGGCAGCGAT CAACCCTTTG CCATCCACAC AGAATGGACC TGTCTTTGCA 
AAAGCAATCC AGAAGAGAGT ACCTTGTGCT TATGACAAGA CTGCCTTGGC ATTGGAGGTT 
GGTGACATTG TGAAAGTCAC AAGGATGAAT ATCAATGGCC AATGGGAAGG CGAGGTGAAT 
GGGCGCAAGG GGCTTTTCCC CTTCACACAT GTTAAAATCT TTGACCCTCA GAACCCCGAT 
GACAACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031790.2
CDS	494..1405
Translation	MSSARFDSSDRSAWYMGPVTRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPVGSVSAPNLPTAEENLEYVRTLYDFPGNDAEDLPFKKGELLVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPTVASTPGAAINPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDDNE

Target ORF information:

RefSeq Version	NM_007764.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus v-crk avian sarcoma virus CT10 oncogene homolog-like (Crkl), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007764.5

ORF Insert Sequence:

ATGTCCTCCG CCAGGTTTGA TTCTTCAGAC CGCTCTGCCT GGTACATGGG GCCAGTGACT 
CGCCAGGAGG CGCAGACTCG TCTCCAAGGC CAGCGCCATG GCATGTTCCT AGTCCGGGAC 
TCATCTACCT GCCCCGGGGA CTATGTACTG TCCGTGTCCG AGAACTCGCG TGTCTCGCAC 
TACATCATCA ACTCCCTGCC CAACCGCCGC TTTAAGATCG GGGACCAGGA GTTTGACCAT 
TTGCCGGCCT TGTTAGAGTT CTACAAGATC CACTACCTGG ACACTACCAC CTTAATCGAA 
CCGGCGCCCA GGTACCCAAG CCCACCAGTG GGTTCTGTCT CAGCACCCAA CTTACCTACA 
GCAGAAGAAA ATCTGGAATA TGTACGGACC CTTTATGATT TTCCTGGGAA TGATGCTGAA 
GACCTACCCT TTAAAAAGGG CGAGCTTCTA GTGATAATAG AAAAGCCTGA AGAGCAGTGG 
TGGAGTGCCC GCAACAAGGA CGGCCGGGTT GGGATGATTC CTGTCCCTTA CGTTGAAAAG 
CTTGTGAGGT CTTCGCCACA TGGAAAGCAT GGAAATAGGA ATTCTAACAG TTATGGCATC 
CCAGAACCTG CTCATGCGTA TGCTCAACCT CAGACCACAA CTCCTCTACC TACAGTTGCC 
AGTACTCCTG GGGCAGCGAT CAACCCTTTG CCATCCACAC AGAATGGACC TGTCTTTGCA 
AAAGCAATCC AGAAGAGAGT ACCTTGTGCT TATGACAAGA CTGCCTTGGC ATTGGAGGTT 
GGTGACATTG TGAAAGTCAC AAGGATGAAT ATCAATGGCC AATGGGAAGG CGAGGTGAAT 
GGGCGCAAGG GGCTTTTCCC CTTCACACAT GTTAAAATCT TTGACCCTCA GAACCCCGAT 
GACAACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crk proteins transduce FGF signaling to promote lens fiber cell elongation.
eLife7(2018 Jan)
Collins TN,Mao Y,Li H,Bouaziz M,Hong A,Feng GS,Wang F,Quilliam LA,Chen L,Park T,Curran T,Zhang X

Murine model indicates 22q11.2 signaling adaptor
Proceedings of the National Academy of Sciences of the United States of America114(19)4981-4986(2017 May)
Haller M,Mo Q,Imamoto A,Lamb DJ

Genetic Drivers of Kidney Defects in the DiGeorge Syndrome.
The New England journal of medicine376(8)742-754(2017 02)
Lopez-Rivera E,Liu YP,Verbitsky M,Anderson BR,Capone VP,Otto EA,Yan Z,Mitrotti A,Martino J,Steers NJ,Fasel DA,Vukojevic K,Deng R,Racedo SE,Liu Q,Werth M,Westland R,Vivante A,Makar GS,Bodria M,Sampson MG,Gillies CE,Vega-Warner V,Maiorana M,Petrey DS,Honig B,Lozanovski VJ,Salomon R,Heidet L,Carpentier W,Gaillard D,Carrea A,Gesualdo L,Cusi D,Izzi C,Scolari F,van Wijk JA,Arapovic A,Saraga-Babic M,Saraga M,Kunac N,Samii A,McDonald-McGinn DM,Crowley TB,Zackai EH,Drozdz D,Miklaszewska M,Tkaczyk M,Sikora P,Szczepanska M,Mizerska-Wasiak M,Krzemien G,Szmigielska A,Zaniew M,Darlow JM,Puri P,Barton D,Casolari E,Furth SL,Warady BA,Gucev Z,Hakonarson H,Flogelova H,Tasic V,Latos-Bielenska A,Materna-Kiryluk A,Allegri L,Wong CS,Drummond IA,D'Agati V,Imamoto A,Barasch JM,Hildebrandt F,Kiryluk K,Lifton RP,Morrow BE,Jeanpierre C,Papaioannou VE,Ghiggeri GM,Gharavi AG,Katsanis N,Sanna-Cherchi S

BCR/ABL P190 transgenic mice develop leukemia in the absence of Crkl.
Oncogene21(20)3225-31(2002 May)
Hemmeryckx B,Reichert A,Watanabe M,Kaartinen V,de Jong R,Pattengale PK,Groffen J,Heisterkamp N

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Crkl cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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