CRKL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRKL
Entrez Gene ID	1399
Full Name	CRK like proto-oncogene, adaptor protein
General protein information	Preferred Names CRK like proto-oncogene, adaptor protein Names crk-like protein v-crk avian sarcoma virus CT10 oncogene homolog-like
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q11.21
Summary	This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009].
Disorder MIM:	602007

mRNA and Protein(s)

mRNA	Protein	Name
NM_005207.4	NP_005198.1	crk-like protein
NM_005207.3	NP_005198.1	crk-like protein

Pathways from BioSystems

Homologies

	CRKL	XP_001086123.1
Gallus gallus (chicken)	CRKL	XP_415233.1
Pan troglodytes (chimpanzee)	CRKL	XP_525530.2
Canis lupus familiaris (dog)	CRKL	XP_849949.1
Xenopus tropicalis (tropical clawed frog)	crkl	NP_989060.1
Mus musculus (house mouse)	Crkl	NP_031790.2
Homo sapiens (human)	CRKL	NP_005198.1
Rattus norvegicus (Norway rat)	Crkl	NP_001008285.1
Rattus norvegicus (Norway rat)	LOC100911248	XP_003751143.1
Danio rerio (zebrafish)	crkl	NP_998703.1

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-379 suppresses cervical cancer cell proliferation and invasion by directly targeting V-crk avian sarcoma virus CT10 oncogene homolog-like.
Shi X, Yuan N, Zhang S, Yuan F, Wang X
Oncology research(2018 Jan)

Dynamic multi-site phosphorylation by Fyn and Abl drives the interaction between CRKL and the novel scaffolding receptors DCBLD1 and DCBLD2.
Schmoker AM, Weinert JL, Kellett KJ, Johnson HE, Joy RM, Weir ME, Ebert AM, Ballif BA
The Biochemical journal474(23)3963-3984(2017 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dynamic multi-site phosphorylation by Fyn and Abl drives the interaction between CRKL and the novel scaffolding receptors DCBLD1 and DCBLD2.
Title: Dynamic multi-site phosphorylation by Fyn and Abl drives the interaction between CRKL and the novel scaffolding receptors DCBLD1 and DCBLD2.

Data show that miR-193b, by directly targeting focal adhesion kinase (FAK), CRK-like proto-oncogene (CRKL), and methionine sulfoxide reductase A (MSRA), regulates focal adhesion signaling and ROS signaling, which play pivotal roles in liposarcomagenesis and adipogenic differentiation.
Title: miR-193b-Regulated Signaling Networks Serve as Tumor Suppressors in Liposarcoma and Promote Adipogenesis in Adipose-Derived Stem Cells.

TP53-miR-215-PCAT-1-CRKL axis might represent an important regulatory pathway in hepatocellular carcinoma.
Title: The long noncoding RNA

We identified a recurrent 370-kb deletion at the 22q11.2 locus as a driver of kidney defects in the DiGeorge syndrome and in sporadic congenital kidney and urinary tract anomalies. SNAP29, AIFM3, and CRKL appear to be critical to the phenotype, with haploinsufficiency of CRKL emerging as the main genetic driver.
Title: Genetic Drivers of Kidney Defects in the DiGeorge Syndrome.

he findings in this study indicate a regulation relationship between CRKL and SLC7A5, and provide useful evidence for gastric cancer therapeutic strategies.
Title: SLC7A5 Functions as a Downstream Target Modulated by CRKL in Metastasis Process of Gastric Cancer SGC-7901 Cells.

The following CRKL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRKL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18349	NM_005207.4 Latest version!	Homo sapiens CRK like proto-oncogene, adaptor protein (CRKL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
View Old Accession Versions >>
OHu18349	NM_005207.3	Homo sapiens CRK like proto-oncogene, adaptor protein (CRKL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRKL ( NM_005207.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005198.1 Homo sapiens CRK like proto-oncogene, adaptor protein (CRKL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18349
Clone ID Related Accession (Same CDS sequence)	NM_005207.4 , NM_005207.3
Accession Version	NM_005207.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	crk-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002470.17, X59656.1, BC043500.1 and AA844269.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005207.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.144276.1, SRR1803615.104356.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000354336.8/ ENSP00000346300.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCTCCG CCAGGTTCGA CTCCTCGGAC CGCTCCGCCT GGTATATGGG GCCGGTGTCT 
CGCCAGGAGG CGCAGACCCG GCTCCAGGGC CAGCGCCACG GTATGTTCCT CGTCCGCGAT 
TCTTCCACCT GCCCTGGGGA CTATGTGCTG TCGGTGTCCG AGAACTCGCG GGTCTCCCAC 
TACATCATCA ACTCGCTGCC CAACCGCCGT TTTAAGATCG GGGACCAGGA ATTTGACCAT 
TTGCCGGCCC TGCTGGAGTT TTACAAGATC CACTACCTGG ACACCACCAC CCTCATCGAG 
CCTGCGCCCA GGTATCCAAG CCCACCAATG GGATCTGTCT CAGCACCCAA CCTGCCTACA 
GCAGAAGATA ACCTGGAATA TGTACGGACT CTGTATGATT TTCCTGGGAA TGATGCCGAA 
GACCTGCCCT TTAAAAAGGG TGAGATCCTA GTGATAATAG AGAAGCCTGA AGAACAGTGG 
TGGAGTGCCC GGAACAAGGA TGGCCGGGTT GGGATGATTC CTGTCCCTTA TGTCGAAAAG 
CTTGTGAGAT CCTCACCACA CGGAAAGCAT GGAAATAGGA ATTCCAACAG TTATGGGATC 
CCAGAACCTG CTCATGCATA CGCTCAACCT CAGACCACAA CTCCTCTACC TGCAGTTTCC 
GGTTCTCCTG GGGCAGCAAT CACCCCTTTG CCATCCACAC AGAATGGACC TGTCTTTGCG 
AAAGCAATCC AGAAAAGAGT ACCCTGTGCT TATGACAAGA CTGCCTTGGC ATTAGAGGTT 
GGTGACATCG TGAAAGTCAC AAGGATGAAT ATAAATGGCC AGTGGGAAGG CGAAGTGAAC 
GGGCGCAAAG GGCTTTTCCC CTTTACGCAC GTCAAAATCT TTGACCCTCA AAACCCAGAT 
GAAAACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005198.1
CDS	529..1440
Translation	MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPMGSVSAPNLPTAEDNLEYVRTLYDFPGNDAEDLPFKKGEILVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDENE

Target ORF information:

RefSeq Version	NM_005207.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CRK like proto-oncogene, adaptor protein (CRKL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005207.4

ORF Insert Sequence:

ATGTCCTCCG CCAGGTTCGA CTCCTCGGAC CGCTCCGCCT GGTATATGGG GCCGGTGTCT 
CGCCAGGAGG CGCAGACCCG GCTCCAGGGC CAGCGCCACG GTATGTTCCT CGTCCGCGAT 
TCTTCCACCT GCCCTGGGGA CTATGTGCTG TCGGTGTCCG AGAACTCGCG GGTCTCCCAC 
TACATCATCA ACTCGCTGCC CAACCGCCGT TTTAAGATCG GGGACCAGGA ATTTGACCAT 
TTGCCGGCCC TGCTGGAGTT TTACAAGATC CACTACCTGG ACACCACCAC CCTCATCGAG 
CCTGCGCCCA GGTATCCAAG CCCACCAATG GGATCTGTCT CAGCACCCAA CCTGCCTACA 
GCAGAAGATA ACCTGGAATA TGTACGGACT CTGTATGATT TTCCTGGGAA TGATGCCGAA 
GACCTGCCCT TTAAAAAGGG TGAGATCCTA GTGATAATAG AGAAGCCTGA AGAACAGTGG 
TGGAGTGCCC GGAACAAGGA TGGCCGGGTT GGGATGATTC CTGTCCCTTA TGTCGAAAAG 
CTTGTGAGAT CCTCACCACA CGGAAAGCAT GGAAATAGGA ATTCCAACAG TTATGGGATC 
CCAGAACCTG CTCATGCATA CGCTCAACCT CAGACCACAA CTCCTCTACC TGCAGTTTCC 
GGTTCTCCTG GGGCAGCAAT CACCCCTTTG CCATCCACAC AGAATGGACC TGTCTTTGCG 
AAAGCAATCC AGAAAAGAGT ACCCTGTGCT TATGACAAGA CTGCCTTGGC ATTAGAGGTT 
GGTGACATCG TGAAAGTCAC AAGGATGAAT ATAAATGGCC AGTGGGAAGG CGAAGTGAAC 
GGGCGCAAAG GGCTTTTCCC CTTTACGCAC GTCAAAATCT TTGACCCTCA AAACCCAGAT 
GAAAACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRKL ( NM_005207.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005198.1 Homo sapiens CRK like proto-oncogene, adaptor protein (CRKL), mRNA.

Price & Availability↑

CloneID	OHu18349
Clone ID Related Accession (Same CDS sequence)	NM_005207.4 , NM_005207.3
Accession Version	NM_005207.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	crk-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X59656.1, BC043500.1, AC002470.17 and AA844269.1. This sequence is a reference standard in the RefSeqGene project. On Jan 6, 2009 this sequence version replaced NM_005207.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X59656.1, SRR1803616.132654.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTCCG CCAGGTTCGA CTCCTCGGAC CGCTCCGCCT GGTATATGGG GCCGGTGTCT 
CGCCAGGAGG CGCAGACCCG GCTCCAGGGC CAGCGCCACG GTATGTTCCT CGTCCGCGAT 
TCTTCCACCT GCCCTGGGGA CTATGTGCTG TCGGTGTCCG AGAACTCGCG GGTCTCCCAC 
TACATCATCA ACTCGCTGCC CAACCGCCGT TTTAAGATCG GGGACCAGGA ATTTGACCAT 
TTGCCGGCCC TGCTGGAGTT TTACAAGATC CACTACCTGG ACACCACCAC CCTCATCGAG 
CCTGCGCCCA GGTATCCAAG CCCACCAATG GGATCTGTCT CAGCACCCAA CCTGCCTACA 
GCAGAAGATA ACCTGGAATA TGTACGGACT CTGTATGATT TTCCTGGGAA TGATGCCGAA 
GACCTGCCCT TTAAAAAGGG TGAGATCCTA GTGATAATAG AGAAGCCTGA AGAACAGTGG 
TGGAGTGCCC GGAACAAGGA TGGCCGGGTT GGGATGATTC CTGTCCCTTA TGTCGAAAAG 
CTTGTGAGAT CCTCACCACA CGGAAAGCAT GGAAATAGGA ATTCCAACAG TTATGGGATC 
CCAGAACCTG CTCATGCATA CGCTCAACCT CAGACCACAA CTCCTCTACC TGCAGTTTCC 
GGTTCTCCTG GGGCAGCAAT CACCCCTTTG CCATCCACAC AGAATGGACC TGTCTTTGCG 
AAAGCAATCC AGAAAAGAGT ACCCTGTGCT TATGACAAGA CTGCCTTGGC ATTAGAGGTT 
GGTGACATCG TGAAAGTCAC AAGGATGAAT ATAAATGGCC AGTGGGAAGG CGAAGTGAAC 
GGGCGCAAAG GGCTTTTCCC CTTTACGCAC GTCAAAATCT TTGACCCTCA AAACCCAGAT 
GAAAACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005198.1
CDS	510..1421
Translation	MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPMGSVSAPNLPTAEDNLEYVRTLYDFPGNDAEDLPFKKGEILVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDENE

Target ORF information:

RefSeq Version	NM_005207.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CRK like proto-oncogene, adaptor protein (CRKL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005207.3

ORF Insert Sequence:

ATGTCCTCCG CCAGGTTCGA CTCCTCGGAC CGCTCCGCCT GGTATATGGG GCCGGTGTCT 
CGCCAGGAGG CGCAGACCCG GCTCCAGGGC CAGCGCCACG GTATGTTCCT CGTCCGCGAT 
TCTTCCACCT GCCCTGGGGA CTATGTGCTG TCGGTGTCCG AGAACTCGCG GGTCTCCCAC 
TACATCATCA ACTCGCTGCC CAACCGCCGT TTTAAGATCG GGGACCAGGA ATTTGACCAT 
TTGCCGGCCC TGCTGGAGTT TTACAAGATC CACTACCTGG ACACCACCAC CCTCATCGAG 
CCTGCGCCCA GGTATCCAAG CCCACCAATG GGATCTGTCT CAGCACCCAA CCTGCCTACA 
GCAGAAGATA ACCTGGAATA TGTACGGACT CTGTATGATT TTCCTGGGAA TGATGCCGAA 
GACCTGCCCT TTAAAAAGGG TGAGATCCTA GTGATAATAG AGAAGCCTGA AGAACAGTGG 
TGGAGTGCCC GGAACAAGGA TGGCCGGGTT GGGATGATTC CTGTCCCTTA TGTCGAAAAG 
CTTGTGAGAT CCTCACCACA CGGAAAGCAT GGAAATAGGA ATTCCAACAG TTATGGGATC 
CCAGAACCTG CTCATGCATA CGCTCAACCT CAGACCACAA CTCCTCTACC TGCAGTTTCC 
GGTTCTCCTG GGGCAGCAAT CACCCCTTTG CCATCCACAC AGAATGGACC TGTCTTTGCG 
AAAGCAATCC AGAAAAGAGT ACCCTGTGCT TATGACAAGA CTGCCTTGGC ATTAGAGGTT 
GGTGACATCG TGAAAGTCAC AAGGATGAAT ATAAATGGCC AGTGGGAAGG CGAAGTGAAC 
GGGCGCAAAG GGCTTTTCCC CTTTACGCAC GTCAAAATCT TTGACCCTCA AAACCCAGAT 
GAAAACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-379 suppresses cervical cancer cell proliferation and invasion by directly targeting V-crk avian sarcoma virus CT10 oncogene homolog-like.
Oncology research(2018 Jan)
Shi X,Yuan N,Zhang S,Yuan F,Wang X

Dynamic multi-site phosphorylation by Fyn and Abl drives the interaction between CRKL and the novel scaffolding receptors DCBLD1 and DCBLD2.
The Biochemical journal474(23)3963-3984(2017 11)
Schmoker AM,Weinert JL,Kellett KJ,Johnson HE,Joy RM,Weir ME,Ebert AM,Ballif BA

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CRKL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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