Mbd5 cDNA ORF clone, Mus musculus(house mouse)

The following Mbd5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mbd5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu17187 XM_030246635.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu72276 XM_030246638.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu72276 XM_017314916.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu21530 XM_030246628.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu21530 XM_030246629.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu21530 XM_030246625.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu21530 XM_030246624.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu21530 XM_030246630.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu21530 XM_030246626.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu21530 XM_017314911.2
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu29239 XM_006497604.3
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu17187 NM_001290656.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu21530 NM_029924.2
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu17187 XM_017314915.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu21530 XM_017314912.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu21530 XM_017314910.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu21530 XM_017314909.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu72275 XM_017314914.1
Latest version!
Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
View Old Accession Versions >>
OMu21530 XM_017314911.1 Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu29239 XM_006497604.2 Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu17187
    Clone ID Related Accession (Same CDS sequence) XM_030246635.1 , XM_017314915.1 , NM_001290656.1
    Accession Version XM_030246635.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GTGATATATC ATCCATAAAC
    AATTCATTGA ATAACCATCA ACTGACTCAT CTACAGTCGC TACTAAACAG CAATCAGATG
    TTTCCTCCAA ATCAGCAGCC GCAGCAACAC CTTCTCCAGG GACACCAGAA TCTCCAGGCC
    TTCCAAGGAC AACCCACAGT CCCTTGCCCA GCTAACAATA ACCCCATGGC TTGTCTGTTT
    CAGAACTTCC AGGTGAGGAT GCAAGGAGAT GCAGCTCTTC TAAATAAAAG AATAAGCACT
    CAGCCTGGGC TCACAACACT TCCTGAAAAC CCAAACCTTG CACTTCCACA TTTCCAAGAT
    ACACCCTGTG AGCTGCAGCC AAGGATTGAC CTTGGTCAGC CTATGAAGGA CGGCCTCGTT
    ATGGGTGGCC AAGGAGATGC CTCCGTAGAC GCCATTTACA AAGCAGTTGT GGATGCAGCC
    AGCAAAGGAA TGCAGGTTGT CATCACCACT GCTGTCAACA GCACGACTCA AATCAGCCCT
    ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC ACTGCCTCCA TTGGTGACCC ATTAAATCTG
    TCCAGTGCTG TGAGCGCTGT CATCCACGGG CGGAACATGG GAGGAGTGGA TCATGATGGC
    AGGCTGAGGA ATGCGAGAGG GGCACGGCTA CCCAAGAACA TAGACCATGG GAAGAACTCC
    AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG TCAGCGAGCT GCCACACATC CAGAAAACAG
    TGGGATGGGG AGCAAAGTCC CAGAGGGGAG CGCAACAGGT GGAAGTATGA GGAGTTTTTA
    GATCATCCAG GCCATATCCA CAGTAGTCCT TGCCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TAGCTGGGGA ACAGCACCCG ATACTGTTAC CACCAAGAAA CTGTCAAGGT
    GATAAGATTC TGGAGGAAAA TTTCAGGTAT AATAATTACA AAAGAACTAT GATGAGTTTT
    AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA TGTACACACA TTAACGGAAA CAGACCTCGT
    CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC ACTGCAAAAC AAGACCTAGT TCTAGAGGGG
    CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AACCATTAAT GGGTGTGTGC CTAGTCCCTC AGATGCTAAA
    AGCATTAGTA GTGAAGACGA CCTACGGAAT CCAGACTCCC CCTCCTCACA TGAGTTGATA
    CACTATAGAC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGGAAATT CATAAGAGAA GACGACGTTC ACAATTCATG CCAACAAAGC
    CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG TTGAAGACTC TCACAGAAGG TTTAGAAGCC
    TACAGCCGCG TCCGGAAAAG GAGCAGAAAA AGTGGGAAAC TAAATAACCA TTTAGAAGCT
    GCTATTCACG AGGCCATGAG CGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCA
    CAGGGTGACA GACAAATGAG GCCCCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030102495.1
    CDS1301..5797
    Translation

    Target ORF information:

    RefSeq Version XM_030246635.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246635.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GTGATATATC ATCCATAAAC
    AATTCATTGA ATAACCATCA ACTGACTCAT CTACAGTCGC TACTAAACAG CAATCAGATG
    TTTCCTCCAA ATCAGCAGCC GCAGCAACAC CTTCTCCAGG GACACCAGAA TCTCCAGGCC
    TTCCAAGGAC AACCCACAGT CCCTTGCCCA GCTAACAATA ACCCCATGGC TTGTCTGTTT
    CAGAACTTCC AGGTGAGGAT GCAAGGAGAT GCAGCTCTTC TAAATAAAAG AATAAGCACT
    CAGCCTGGGC TCACAACACT TCCTGAAAAC CCAAACCTTG CACTTCCACA TTTCCAAGAT
    ACACCCTGTG AGCTGCAGCC AAGGATTGAC CTTGGTCAGC CTATGAAGGA CGGCCTCGTT
    ATGGGTGGCC AAGGAGATGC CTCCGTAGAC GCCATTTACA AAGCAGTTGT GGATGCAGCC
    AGCAAAGGAA TGCAGGTTGT CATCACCACT GCTGTCAACA GCACGACTCA AATCAGCCCT
    ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC ACTGCCTCCA TTGGTGACCC ATTAAATCTG
    TCCAGTGCTG TGAGCGCTGT CATCCACGGG CGGAACATGG GAGGAGTGGA TCATGATGGC
    AGGCTGAGGA ATGCGAGAGG GGCACGGCTA CCCAAGAACA TAGACCATGG GAAGAACTCC
    AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG TCAGCGAGCT GCCACACATC CAGAAAACAG
    TGGGATGGGG AGCAAAGTCC CAGAGGGGAG CGCAACAGGT GGAAGTATGA GGAGTTTTTA
    GATCATCCAG GCCATATCCA CAGTAGTCCT TGCCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TAGCTGGGGA ACAGCACCCG ATACTGTTAC CACCAAGAAA CTGTCAAGGT
    GATAAGATTC TGGAGGAAAA TTTCAGGTAT AATAATTACA AAAGAACTAT GATGAGTTTT
    AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA TGTACACACA TTAACGGAAA CAGACCTCGT
    CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC ACTGCAAAAC AAGACCTAGT TCTAGAGGGG
    CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AACCATTAAT GGGTGTGTGC CTAGTCCCTC AGATGCTAAA
    AGCATTAGTA GTGAAGACGA CCTACGGAAT CCAGACTCCC CCTCCTCACA TGAGTTGATA
    CACTATAGAC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGGAAATT CATAAGAGAA GACGACGTTC ACAATTCATG CCAACAAAGC
    CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG TTGAAGACTC TCACAGAAGG TTTAGAAGCC
    TACAGCCGCG TCCGGAAAAG GAGCAGAAAA AGTGGGAAAC TAAATAACCA TTTAGAAGCT
    GCTATTCACG AGGCCATGAG CGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCA
    CAGGGTGACA GACAAATGAG GCCCCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72276
    Clone ID Related Accession (Same CDS sequence) XM_030246638.1 , XM_017314916.1
    Accession Version XM_030246638.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGGAGCC CGAGAAGTTG
    AAGACTCTCA CAGAAGGTTT AGAAGCCTAC AGCCGCGTCC GGAAAAGGAG CAGAAAAAGT
    GGGAAACTAA ATAACCATTT AGAAGCTGCT ATTCACGAGG CCATGAGCGA ACTGGACAAA
    ATGTCTGGGA CTGTTCACCA AATCCCACAG GGTGACAGAC AAATGAGGCC CCCCAAACCC
    AAGAGGAGGA AGATCTCCAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030102498.1
    CDS1301..5284
    Translation

    Target ORF information:

    RefSeq Version XM_030246638.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246638.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGGAGCC CGAGAAGTTG
    AAGACTCTCA CAGAAGGTTT AGAAGCCTAC AGCCGCGTCC GGAAAAGGAG CAGAAAAAGT
    GGGAAACTAA ATAACCATTT AGAAGCTGCT ATTCACGAGG CCATGAGCGA ACTGGACAAA
    ATGTCTGGGA CTGTTCACCA AATCCCACAG GGTGACAGAC AAATGAGGCC CCCCAAACCC
    AAGAGGAGGA AGATCTCCAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72276
    Clone ID Related Accession (Same CDS sequence) XM_030246638.1 , XM_017314916.1
    Accession Version XM_017314916.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGGAGCC CGAGAAGTTG
    AAGACTCTCA CAGAAGGTTT AGAAGCCTAC AGCCGCGTCC GGAAAAGGAG CAGAAAAAGT
    GGGAAACTAA ATAACCATTT AGAAGCTGCT ATTCACGAGG CCATGAGCGA ACTGGACAAA
    ATGTCTGGGA CTGTTCACCA AATCCCACAG GGTGACAGAC AAATGAGGCC CCCCAAACCC
    AAGAGGAGGA AGATCTCCAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170405.1
    CDS1269..5252
    Translation

    Target ORF information:

    RefSeq Version XM_017314916.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314916.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGGAGCC CGAGAAGTTG
    AAGACTCTCA CAGAAGGTTT AGAAGCCTAC AGCCGCGTCC GGAAAAGGAG CAGAAAAAGT
    GGGAAACTAA ATAACCATTT AGAAGCTGCT ATTCACGAGG CCATGAGCGA ACTGGACAAA
    ATGTCTGGGA CTGTTCACCA AATCCCACAG GGTGACAGAC AAATGAGGCC CCCCAAACCC
    AAGAGGAGGA AGATCTCCAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21530
    Clone ID Related Accession (Same CDS sequence) XM_030246630.1 , XM_017314910.1 , XM_030246625.1 , XM_030246626.1 , XM_030246628.1 , XM_017314912.1 , NM_029924.2 , XM_030246624.1 , XM_030246629.1 , XM_017314911.2 , XM_017314909.1 , XM_017314911.1
    Accession Version XM_030246630.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030102490.1
    CDS1210..6396
    Translation

    Target ORF information:

    RefSeq Version XM_030246630.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21530
    Clone ID Related Accession (Same CDS sequence) XM_030246630.1 , XM_017314910.1 , XM_030246625.1 , XM_030246626.1 , XM_030246628.1 , XM_017314912.1 , NM_029924.2 , XM_030246624.1 , XM_030246629.1 , XM_017314911.2 , XM_017314909.1 , XM_017314911.1
    Accession Version XM_030246625.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030102485.1
    CDS1477..6663
    Translation

    Target ORF information:

    RefSeq Version XM_030246625.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246625.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21530
    Clone ID Related Accession (Same CDS sequence) XM_030246630.1 , XM_017314910.1 , XM_030246625.1 , XM_030246626.1 , XM_030246628.1 , XM_017314912.1 , NM_029924.2 , XM_030246624.1 , XM_030246629.1 , XM_017314911.2 , XM_017314909.1 , XM_017314911.1
    Accession Version XM_030246626.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030102486.1
    CDS1343..6529
    Translation

    Target ORF information:

    RefSeq Version XM_030246626.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246626.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21530
    Clone ID Related Accession (Same CDS sequence) XM_030246630.1 , XM_017314910.1 , XM_030246625.1 , XM_030246626.1 , XM_030246628.1 , XM_017314912.1 , NM_029924.2 , XM_030246624.1 , XM_030246629.1 , XM_017314911.2 , XM_017314909.1 , XM_017314911.1
    Accession Version XM_030246628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030102488.1
    CDS1417..6603
    Translation

    Target ORF information:

    RefSeq Version XM_030246628.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21530
    Clone ID Related Accession (Same CDS sequence) XM_030246630.1 , XM_017314910.1 , XM_030246625.1 , XM_030246626.1 , XM_030246628.1 , XM_017314912.1 , NM_029924.2 , XM_030246624.1 , XM_030246629.1 , XM_017314911.2 , XM_017314909.1 , XM_017314911.1
    Accession Version XM_030246624.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030102484.1
    CDS1301..6487
    Translation

    Target ORF information:

    RefSeq Version XM_030246624.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21530
    Clone ID Related Accession (Same CDS sequence) XM_030246630.1 , XM_017314910.1 , XM_030246625.1 , XM_030246626.1 , XM_030246628.1 , XM_017314912.1 , NM_029924.2 , XM_030246624.1 , XM_030246629.1 , XM_017314911.2 , XM_017314909.1 , XM_017314911.1
    Accession Version XM_030246629.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030102489.1
    CDS1238..6424
    Translation

    Target ORF information:

    RefSeq Version XM_030246629.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21530
    Clone ID Related Accession (Same CDS sequence) XM_030246630.1 , XM_017314910.1 , XM_030246625.1 , XM_030246626.1 , XM_030246628.1 , XM_017314912.1 , NM_029924.2 , XM_030246624.1 , XM_030246629.1 , XM_017314911.2 , XM_017314909.1 , XM_017314911.1
    Accession Version XM_017314911.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314911.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170400.1
    CDS1224..6410
    Translation

    Target ORF information:

    RefSeq Version XM_017314911.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314911.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29239
    Clone ID Related Accession (Same CDS sequence) XM_006497604.2 , XM_006497604.3
    Accession Version XM_006497604.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497604.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCAAGTGT GGCTTGGAAT GTCCTCTTAT TCTTCCCAAG ATGCAACTCC AGTAGTACCT 
    TCACGGGCGG CAACTCCAAG ATCAGTAAGA AACAAGTCTC ATGAAGGAAT TACAAATTCT
    GTAATGCCAG AATGTAAGAA TCCTTTCAAG TTAATGACTG GATCATCAAA TGCCATGGGA
    AGGCTATACA TGCAAGACCT GCCTGGAAGC CAGCAGCAGG AGCTCCACCC TGTCTACCCC
    CGCCAGAGAT TGGGCAGTAG TGAGCATGGA CAGAAATCTC CATTTCGTGG CAGCCATGGA
    GGCCTGCCTA GCCCAGCATC ATCAGGGTCT CAGATCTATG GAGATGGCTC AATCTCTCCA
    AGGACTGACC CGCTCGGAAG CCCTGATGTC TTCACAAGAA ATAACCCTGG TTTTCATGGA
    GCTCCCAATT CCAGCCCTAT TCACCTAAAT AGGACTCCTC TTTCTCCACC TTCAGTAATG
    CTACATGGTT CTCCCGTACA GTCATCCTGT GCAATGGCTG GAAGAACTAA TATACCTCTT
    TCCCCAACCT TGACTACAAA GAGTCCAGTA ATGAAAAAAC CAATGTGTAA TTTTTCAACT
    AATATGGAAA TACCACGAGC AATGTTCCAC CACAAACCAC CCCAAGGCCC ACCTCCCCCT
    CCTCCACCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CTGAGAAAGA TCCACTTGGC
    ATTCTTGACC CTATTCCTAG TAAACCAGTG AATCAGAACC CTATTATCAT TAATCCAACC
    AGTTTCCATT CAAATGTCCA CTCTCAGGTA CCTGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTTGTTCCTT TGCCAAGTAA TCTCCCTTTG CCAACTGTAA AACCTGGTCA TATGAATCAT
    GGGAGTCATG TACAAAGAAT TCAGCATTCA GCTTCAACCT CCCTGTCCCC TTCTCCAGTG
    ACATCCCCAG TGCACATGAT GGGGACTGGA ATTGGAAGGA TTGAGGCATC GCCCCAAAGA
    TCACGCTCAT CTTCCACATC ATCAGATCAT GGAAATTTCA TGATGCCACC TGTAGGACCC
    CAGGCCACTT GTAGTGGCAT AAAGGTTCCA CCTAGGTCAC CAAGGTCAAC AATAGGTTCC
    CCAAGGCCAT CAATGCCATC AAGCCCTTCT ACCAAGTCTG ATGGACATCA TCAGTACAAG
    GATATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAC TCAATACCCC AAGCAGTGCA
    GCTTTTCCTA CTGCACCTGC CGGAAATGGT TCTGTAAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CCTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAACA AACTTGCTGG TAACAACAGT
    AGCAGCAGTA ACAATTCTGG AGCTGTTGCC AGCAGCGGCA ACACTGAAGG ACACAGCACT
    TTAAACACCA TGTTCCCTCC AACTGCCAAC ATGCTTCTCC CAACAGGTGA AGGTCAAAGT
    GGTCGAGCAG CACTAAGAGA TAAGTTGATG TCTCAGCAAA AAGACTCATT ACGGAAAAGA
    AAGCAGCCAC CCACCACAGT GTTGAGTTTG CTCAGACAGT CCCAAATGGA TAGTTCTGCA
    GCTCCTAAAC CTGGACCTGA CTTGCTAAGG AAGCATGGGC AGGGGTCATT TCCCATCAGT
    TCAATGTCTC AGTTACTACA GTCTATGAGT TGTCAAAGCT CTCACTTGAG TAGCAATAGT
    ACCCCGGGTT GTGGGGGATC AAATACTGCT TTGCCTTGCT CTGCTAACCA GCTGCATTTT
    CCAGATCCTA ATATGAATTC CACTGTTCTT CAGAATTCAC TGACACAGAG CATACCTTTA
    AGAGGGGAAG CCGTGCACTG CCACAATGCA AACACTAACT TTGTTCACAG TAACAGTCCA
    GTCCCCAACC ACCATCTTGC AGGTTTAATA AATCAGATTC AGGCTAGCGG GAACTGTGGG
    ATGCTCAGTC AGTCGGGCAT GGCTTTAGGA AACTCATTAC ATCCCAATCC ACCTCAGTCA
    AGAATTTCAA CGTCCTCCAC TCCAGTGATA CCAAACAGCA TTGTTAGCAG CTATAATCAA
    ACAAGTTCTG AAGCAGGTGG TTCAAGCCTG CCGTCCTCCA TAGCTATAGC TGGCAGCAAC
    CACCCTGCCA TCACAAAGAC AACATCTGTT CTCCAAGATG GTGTCATAGT CACCACCGCA
    GCTGGAAACC CACTGCAGAG TCAGCTGCCC ATTGGGAGTG ACTTTCCTTT TGTTGGCCAA
    GAGCATGCCC TTCATTTTCC ATCCAACAGC ACCGCAAACA ACCATCTTCC ACACCCCTTG
    AACCCCAGCC TCCTCAGCTC TCTACCTATC TCTTTGCCAG TGAATCAACA GCATCTCCTA
    AACCAGAATC TATTAAATAT CCTCCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGATCAAC
    CTCCACCCTT TAGGTTTTCT CAACCCGAAT GTAAACGCTG CTTTAGCTTT CCTCTCCGGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTC CACTTTCAGC TCTTAGCAGC CCTGCTTCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTGCCGTCTT TCAATCTGAC CATCTCAGAT
    CTTTTGCAAC CGCAAAATAC CCCTTTACCC TTATTAACAC AGATGACAGC CCCACCAGAC
    CGTTTGCCAA GCAATCAGTC AGACAACAGC CGAGCTGATA CCCTTTTAGG CAGCCCCCTG
    GGAAACCCTT TAGCAGGCAG TGACACTACT TTTAACCCCC TGCTCCTCCC AGCTGTCACT
    GGGGCCTCAG GATTAATGGC CTTGAATCCC CAGCTGTTGG GGGGTGTCCT GAACTCGGCA
    TCGGCCAACA CCGCTATTCA TCCAGAGGTT TCCATAGCAA CCTCCTCCCA GGCAACCACT
    ACCACAACCA CTACATCATC AGCAGTGGCA GCACTGACTG TCTCAACACT TGGTGGGACA
    GCAGTGGTGT CAATGGCAGA AACATTGCTG AATATATCTA ATAATGCTGG GAATCCACCT
    GGTCCAGCTA AACTCAACAG TAACTCTATG GTGCCACAGC TACTTAACCC TCTACTGGGG
    ACAGGTCTGC TTGGTGATAT ATCATCCATA AACAATTCAT TGAATAACCA TCAACTGACT
    CATCTACAGT CGCTACTAAA CAGCAATCAG ATGTTTCCTC CAAATCAGCA GCCGCAGCAA
    CACCTTCTCC AGGGACACCA GAATCTCCAG GCCTTCCAAG GACAACCCAC AGTCCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAACT TCCAGGTGAG GATGCAAGGA
    GATGCAGCTC TTCTAAATAA AAGAATAAGC ACTCAGCCTG GGCTCACAAC ACTTCCTGAA
    AACCCAAACC TTGCACTTCC ACATTTCCAA GATACACCCT GTGAGCTGCA GCCAAGGATT
    GACCTTGGTC AGCCTATGAA GGACGGCCTC GTTATGGGTG GCCAAGGAGA TGCCTCCGTA
    GACGCCATTT ACAAAGCAGT TGTGGATGCA GCCAGCAAAG GAATGCAGGT TGTCATCACC
    ACTGCTGTCA ACAGCACGAC TCAAATCAGC CCTATCCCAG CCCTGAGCGC CATGAGTGCC
    TTCACTGCCT CCATTGGTGA CCCATTAAAT CTGTCCAGTG CTGTGAGCGC TGTCATCCAC
    GGGCGGAACA TGGGAGGAGT GGATCATGAT GGCAGGCTGA GGAATGCGAG AGGGGCACGG
    CTACCCAAGA ACATAGACCA TGGGAAGAAC TCCAGCGAAG GAGACGGGTT TGAGTGTTTC
    AAGTCAGCGA GCTGCCACAC ATCCAGAAAA CAGTGGGATG GGGAGCAAAG TCCCAGAGGG
    GAGCGCAACA GGTGGAAGTA TGAGGAGTTT TTAGATCATC CAGGCCATAT CCACAGTAGT
    CCTTGCCACG AAAGGCCCAA CAATGTCTCT ACACTGCCAT TTCTAGCTGG GGAACAGCAC
    CCGATACTGT TACCACCAAG AAACTGTCAA GGTGATAAGA TTCTGGAGGA AAATTTCAGG
    TATAATAATT ACAAAAGAAC TATGATGAGT TTTAAAGAGA GACTAGAGAG TACTGTGGAG
    AGATGTACAC ACATTAACGG AAACAGACCT CGTCAGAGTC GGGGCTTTGG AGAGCTGCTA
    GGCACTGCAA AACAAGACCT AGTTCTAGAG GGGCAGTCTC CGGGTTCTTC AAATAGTTTG
    GAAAGTTCTC TGGTCAAAGA CTACATCCAT TACAATGGAG ACTTTAATGC CAAAACCATT
    AATGGGTGTG TGCCTAGTCC CTCAGATGCT AAAAGCATTA GTAGTGAAGA CGACCTACGG
    AATCCAGACT CCCCCTCCTC ACATGAGTTG ATACACTATA GACCAAGGAC GTTCAACGTT
    GGCGACTTGG TCTGGGGCCA AATCAAAGGA CTGACTTCCT GGCCTGGGAA ATTCATAAGA
    GAAGACGACG TTCACAATTC ATGCCAACAA AGCCCCGAGG AAGGGAAGGT GGAGCCCGAG
    AAGTTGAAGA CTCTCACAGA AGGTTTAGAA GCCTACAGCC GCGTCCGGAA AAGGAGCAGA
    AAAAGTGGGA AACTAAATAA CCATTTAGAA GCTGCTATTC ACGAGGCCAT GAGCGAACTG
    GACAAAATGT CTGGGACTGT TCACCAAATC CCACAGGGTG ACAGACAAAT GAGGCCCCCC
    AAACCCAAGA GGAGGAAGAT CTCCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497667.1
    CDS1477..6306
    Translation

    Target ORF information:

    RefSeq Version XM_006497604.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006497604.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCAAGTGT GGCTTGGAAT GTCCTCTTAT TCTTCCCAAG ATGCAACTCC AGTAGTACCT 
    TCACGGGCGG CAACTCCAAG ATCAGTAAGA AACAAGTCTC ATGAAGGAAT TACAAATTCT
    GTAATGCCAG AATGTAAGAA TCCTTTCAAG TTAATGACTG GATCATCAAA TGCCATGGGA
    AGGCTATACA TGCAAGACCT GCCTGGAAGC CAGCAGCAGG AGCTCCACCC TGTCTACCCC
    CGCCAGAGAT TGGGCAGTAG TGAGCATGGA CAGAAATCTC CATTTCGTGG CAGCCATGGA
    GGCCTGCCTA GCCCAGCATC ATCAGGGTCT CAGATCTATG GAGATGGCTC AATCTCTCCA
    AGGACTGACC CGCTCGGAAG CCCTGATGTC TTCACAAGAA ATAACCCTGG TTTTCATGGA
    GCTCCCAATT CCAGCCCTAT TCACCTAAAT AGGACTCCTC TTTCTCCACC TTCAGTAATG
    CTACATGGTT CTCCCGTACA GTCATCCTGT GCAATGGCTG GAAGAACTAA TATACCTCTT
    TCCCCAACCT TGACTACAAA GAGTCCAGTA ATGAAAAAAC CAATGTGTAA TTTTTCAACT
    AATATGGAAA TACCACGAGC AATGTTCCAC CACAAACCAC CCCAAGGCCC ACCTCCCCCT
    CCTCCACCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CTGAGAAAGA TCCACTTGGC
    ATTCTTGACC CTATTCCTAG TAAACCAGTG AATCAGAACC CTATTATCAT TAATCCAACC
    AGTTTCCATT CAAATGTCCA CTCTCAGGTA CCTGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTTGTTCCTT TGCCAAGTAA TCTCCCTTTG CCAACTGTAA AACCTGGTCA TATGAATCAT
    GGGAGTCATG TACAAAGAAT TCAGCATTCA GCTTCAACCT CCCTGTCCCC TTCTCCAGTG
    ACATCCCCAG TGCACATGAT GGGGACTGGA ATTGGAAGGA TTGAGGCATC GCCCCAAAGA
    TCACGCTCAT CTTCCACATC ATCAGATCAT GGAAATTTCA TGATGCCACC TGTAGGACCC
    CAGGCCACTT GTAGTGGCAT AAAGGTTCCA CCTAGGTCAC CAAGGTCAAC AATAGGTTCC
    CCAAGGCCAT CAATGCCATC AAGCCCTTCT ACCAAGTCTG ATGGACATCA TCAGTACAAG
    GATATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAC TCAATACCCC AAGCAGTGCA
    GCTTTTCCTA CTGCACCTGC CGGAAATGGT TCTGTAAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CCTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAACA AACTTGCTGG TAACAACAGT
    AGCAGCAGTA ACAATTCTGG AGCTGTTGCC AGCAGCGGCA ACACTGAAGG ACACAGCACT
    TTAAACACCA TGTTCCCTCC AACTGCCAAC ATGCTTCTCC CAACAGGTGA AGGTCAAAGT
    GGTCGAGCAG CACTAAGAGA TAAGTTGATG TCTCAGCAAA AAGACTCATT ACGGAAAAGA
    AAGCAGCCAC CCACCACAGT GTTGAGTTTG CTCAGACAGT CCCAAATGGA TAGTTCTGCA
    GCTCCTAAAC CTGGACCTGA CTTGCTAAGG AAGCATGGGC AGGGGTCATT TCCCATCAGT
    TCAATGTCTC AGTTACTACA GTCTATGAGT TGTCAAAGCT CTCACTTGAG TAGCAATAGT
    ACCCCGGGTT GTGGGGGATC AAATACTGCT TTGCCTTGCT CTGCTAACCA GCTGCATTTT
    CCAGATCCTA ATATGAATTC CACTGTTCTT CAGAATTCAC TGACACAGAG CATACCTTTA
    AGAGGGGAAG CCGTGCACTG CCACAATGCA AACACTAACT TTGTTCACAG TAACAGTCCA
    GTCCCCAACC ACCATCTTGC AGGTTTAATA AATCAGATTC AGGCTAGCGG GAACTGTGGG
    ATGCTCAGTC AGTCGGGCAT GGCTTTAGGA AACTCATTAC ATCCCAATCC ACCTCAGTCA
    AGAATTTCAA CGTCCTCCAC TCCAGTGATA CCAAACAGCA TTGTTAGCAG CTATAATCAA
    ACAAGTTCTG AAGCAGGTGG TTCAAGCCTG CCGTCCTCCA TAGCTATAGC TGGCAGCAAC
    CACCCTGCCA TCACAAAGAC AACATCTGTT CTCCAAGATG GTGTCATAGT CACCACCGCA
    GCTGGAAACC CACTGCAGAG TCAGCTGCCC ATTGGGAGTG ACTTTCCTTT TGTTGGCCAA
    GAGCATGCCC TTCATTTTCC ATCCAACAGC ACCGCAAACA ACCATCTTCC ACACCCCTTG
    AACCCCAGCC TCCTCAGCTC TCTACCTATC TCTTTGCCAG TGAATCAACA GCATCTCCTA
    AACCAGAATC TATTAAATAT CCTCCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGATCAAC
    CTCCACCCTT TAGGTTTTCT CAACCCGAAT GTAAACGCTG CTTTAGCTTT CCTCTCCGGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTC CACTTTCAGC TCTTAGCAGC CCTGCTTCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTGCCGTCTT TCAATCTGAC CATCTCAGAT
    CTTTTGCAAC CGCAAAATAC CCCTTTACCC TTATTAACAC AGATGACAGC CCCACCAGAC
    CGTTTGCCAA GCAATCAGTC AGACAACAGC CGAGCTGATA CCCTTTTAGG CAGCCCCCTG
    GGAAACCCTT TAGCAGGCAG TGACACTACT TTTAACCCCC TGCTCCTCCC AGCTGTCACT
    GGGGCCTCAG GATTAATGGC CTTGAATCCC CAGCTGTTGG GGGGTGTCCT GAACTCGGCA
    TCGGCCAACA CCGCTATTCA TCCAGAGGTT TCCATAGCAA CCTCCTCCCA GGCAACCACT
    ACCACAACCA CTACATCATC AGCAGTGGCA GCACTGACTG TCTCAACACT TGGTGGGACA
    GCAGTGGTGT CAATGGCAGA AACATTGCTG AATATATCTA ATAATGCTGG GAATCCACCT
    GGTCCAGCTA AACTCAACAG TAACTCTATG GTGCCACAGC TACTTAACCC TCTACTGGGG
    ACAGGTCTGC TTGGTGATAT ATCATCCATA AACAATTCAT TGAATAACCA TCAACTGACT
    CATCTACAGT CGCTACTAAA CAGCAATCAG ATGTTTCCTC CAAATCAGCA GCCGCAGCAA
    CACCTTCTCC AGGGACACCA GAATCTCCAG GCCTTCCAAG GACAACCCAC AGTCCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAACT TCCAGGTGAG GATGCAAGGA
    GATGCAGCTC TTCTAAATAA AAGAATAAGC ACTCAGCCTG GGCTCACAAC ACTTCCTGAA
    AACCCAAACC TTGCACTTCC ACATTTCCAA GATACACCCT GTGAGCTGCA GCCAAGGATT
    GACCTTGGTC AGCCTATGAA GGACGGCCTC GTTATGGGTG GCCAAGGAGA TGCCTCCGTA
    GACGCCATTT ACAAAGCAGT TGTGGATGCA GCCAGCAAAG GAATGCAGGT TGTCATCACC
    ACTGCTGTCA ACAGCACGAC TCAAATCAGC CCTATCCCAG CCCTGAGCGC CATGAGTGCC
    TTCACTGCCT CCATTGGTGA CCCATTAAAT CTGTCCAGTG CTGTGAGCGC TGTCATCCAC
    GGGCGGAACA TGGGAGGAGT GGATCATGAT GGCAGGCTGA GGAATGCGAG AGGGGCACGG
    CTACCCAAGA ACATAGACCA TGGGAAGAAC TCCAGCGAAG GAGACGGGTT TGAGTGTTTC
    AAGTCAGCGA GCTGCCACAC ATCCAGAAAA CAGTGGGATG GGGAGCAAAG TCCCAGAGGG
    GAGCGCAACA GGTGGAAGTA TGAGGAGTTT TTAGATCATC CAGGCCATAT CCACAGTAGT
    CCTTGCCACG AAAGGCCCAA CAATGTCTCT ACACTGCCAT TTCTAGCTGG GGAACAGCAC
    CCGATACTGT TACCACCAAG AAACTGTCAA GGTGATAAGA TTCTGGAGGA AAATTTCAGG
    TATAATAATT ACAAAAGAAC TATGATGAGT TTTAAAGAGA GACTAGAGAG TACTGTGGAG
    AGATGTACAC ACATTAACGG AAACAGACCT CGTCAGAGTC GGGGCTTTGG AGAGCTGCTA
    GGCACTGCAA AACAAGACCT AGTTCTAGAG GGGCAGTCTC CGGGTTCTTC AAATAGTTTG
    GAAAGTTCTC TGGTCAAAGA CTACATCCAT TACAATGGAG ACTTTAATGC CAAAACCATT
    AATGGGTGTG TGCCTAGTCC CTCAGATGCT AAAAGCATTA GTAGTGAAGA CGACCTACGG
    AATCCAGACT CCCCCTCCTC ACATGAGTTG ATACACTATA GACCAAGGAC GTTCAACGTT
    GGCGACTTGG TCTGGGGCCA AATCAAAGGA CTGACTTCCT GGCCTGGGAA ATTCATAAGA
    GAAGACGACG TTCACAATTC ATGCCAACAA AGCCCCGAGG AAGGGAAGGT GGAGCCCGAG
    AAGTTGAAGA CTCTCACAGA AGGTTTAGAA GCCTACAGCC GCGTCCGGAA AAGGAGCAGA
    AAAAGTGGGA AACTAAATAA CCATTTAGAA GCTGCTATTC ACGAGGCCAT GAGCGAACTG
    GACAAAATGT CTGGGACTGT TCACCAAATC CCACAGGGTG ACAGACAAAT GAGGCCCCCC
    AAACCCAAGA GGAGGAAGAT CTCCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17187
    Clone ID Related Accession (Same CDS sequence) XM_030246635.1 , XM_017314915.1 , NM_001290656.1
    Accession Version NM_001290656.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-11
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845298.5, CB522230.1, BQ551845.1, BU610422.1, AV298746.2 and BX546465.7. On Mar 15, 2014 this sequence version replaced XM_006497605.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. The 5' UTR of this variant is incomplete due to a lack of full-length transcript support and splice pattern ambiguity in the upstream region. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC138375.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GTGATATATC ATCCATAAAC
    AATTCATTGA ATAACCATCA ACTGACTCAT CTACAGTCGC TACTAAACAG CAATCAGATG
    TTTCCTCCAA ATCAGCAGCC GCAGCAACAC CTTCTCCAGG GACACCAGAA TCTCCAGGCC
    TTCCAAGGAC AACCCACAGT CCCTTGCCCA GCTAACAATA ACCCCATGGC TTGTCTGTTT
    CAGAACTTCC AGGTGAGGAT GCAAGGAGAT GCAGCTCTTC TAAATAAAAG AATAAGCACT
    CAGCCTGGGC TCACAACACT TCCTGAAAAC CCAAACCTTG CACTTCCACA TTTCCAAGAT
    ACACCCTGTG AGCTGCAGCC AAGGATTGAC CTTGGTCAGC CTATGAAGGA CGGCCTCGTT
    ATGGGTGGCC AAGGAGATGC CTCCGTAGAC GCCATTTACA AAGCAGTTGT GGATGCAGCC
    AGCAAAGGAA TGCAGGTTGT CATCACCACT GCTGTCAACA GCACGACTCA AATCAGCCCT
    ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC ACTGCCTCCA TTGGTGACCC ATTAAATCTG
    TCCAGTGCTG TGAGCGCTGT CATCCACGGG CGGAACATGG GAGGAGTGGA TCATGATGGC
    AGGCTGAGGA ATGCGAGAGG GGCACGGCTA CCCAAGAACA TAGACCATGG GAAGAACTCC
    AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG TCAGCGAGCT GCCACACATC CAGAAAACAG
    TGGGATGGGG AGCAAAGTCC CAGAGGGGAG CGCAACAGGT GGAAGTATGA GGAGTTTTTA
    GATCATCCAG GCCATATCCA CAGTAGTCCT TGCCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TAGCTGGGGA ACAGCACCCG ATACTGTTAC CACCAAGAAA CTGTCAAGGT
    GATAAGATTC TGGAGGAAAA TTTCAGGTAT AATAATTACA AAAGAACTAT GATGAGTTTT
    AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA TGTACACACA TTAACGGAAA CAGACCTCGT
    CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC ACTGCAAAAC AAGACCTAGT TCTAGAGGGG
    CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AACCATTAAT GGGTGTGTGC CTAGTCCCTC AGATGCTAAA
    AGCATTAGTA GTGAAGACGA CCTACGGAAT CCAGACTCCC CCTCCTCACA TGAGTTGATA
    CACTATAGAC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGGAAATT CATAAGAGAA GACGACGTTC ACAATTCATG CCAACAAAGC
    CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG TTGAAGACTC TCACAGAAGG TTTAGAAGCC
    TACAGCCGCG TCCGGAAAAG GAGCAGAAAA AGTGGGAAAC TAAATAACCA TTTAGAAGCT
    GCTATTCACG AGGCCATGAG CGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCA
    CAGGGTGACA GACAAATGAG GCCCCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277585.1
    CDS195..4691
    Translation

    Target ORF information:

    RefSeq Version NM_001290656.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290656.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GTGATATATC ATCCATAAAC
    AATTCATTGA ATAACCATCA ACTGACTCAT CTACAGTCGC TACTAAACAG CAATCAGATG
    TTTCCTCCAA ATCAGCAGCC GCAGCAACAC CTTCTCCAGG GACACCAGAA TCTCCAGGCC
    TTCCAAGGAC AACCCACAGT CCCTTGCCCA GCTAACAATA ACCCCATGGC TTGTCTGTTT
    CAGAACTTCC AGGTGAGGAT GCAAGGAGAT GCAGCTCTTC TAAATAAAAG AATAAGCACT
    CAGCCTGGGC TCACAACACT TCCTGAAAAC CCAAACCTTG CACTTCCACA TTTCCAAGAT
    ACACCCTGTG AGCTGCAGCC AAGGATTGAC CTTGGTCAGC CTATGAAGGA CGGCCTCGTT
    ATGGGTGGCC AAGGAGATGC CTCCGTAGAC GCCATTTACA AAGCAGTTGT GGATGCAGCC
    AGCAAAGGAA TGCAGGTTGT CATCACCACT GCTGTCAACA GCACGACTCA AATCAGCCCT
    ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC ACTGCCTCCA TTGGTGACCC ATTAAATCTG
    TCCAGTGCTG TGAGCGCTGT CATCCACGGG CGGAACATGG GAGGAGTGGA TCATGATGGC
    AGGCTGAGGA ATGCGAGAGG GGCACGGCTA CCCAAGAACA TAGACCATGG GAAGAACTCC
    AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG TCAGCGAGCT GCCACACATC CAGAAAACAG
    TGGGATGGGG AGCAAAGTCC CAGAGGGGAG CGCAACAGGT GGAAGTATGA GGAGTTTTTA
    GATCATCCAG GCCATATCCA CAGTAGTCCT TGCCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TAGCTGGGGA ACAGCACCCG ATACTGTTAC CACCAAGAAA CTGTCAAGGT
    GATAAGATTC TGGAGGAAAA TTTCAGGTAT AATAATTACA AAAGAACTAT GATGAGTTTT
    AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA TGTACACACA TTAACGGAAA CAGACCTCGT
    CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC ACTGCAAAAC AAGACCTAGT TCTAGAGGGG
    CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AACCATTAAT GGGTGTGTGC CTAGTCCCTC AGATGCTAAA
    AGCATTAGTA GTGAAGACGA CCTACGGAAT CCAGACTCCC CCTCCTCACA TGAGTTGATA
    CACTATAGAC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGGAAATT CATAAGAGAA GACGACGTTC ACAATTCATG CCAACAAAGC
    CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG TTGAAGACTC TCACAGAAGG TTTAGAAGCC
    TACAGCCGCG TCCGGAAAAG GAGCAGAAAA AGTGGGAAAC TAAATAACCA TTTAGAAGCT
    GCTATTCACG AGGCCATGAG CGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCA
    CAGGGTGACA GACAAATGAG GCCCCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21530
    Clone ID Related Accession (Same CDS sequence) XM_030246630.1 , XM_017314910.1 , XM_030246625.1 , XM_030246626.1 , XM_030246628.1 , XM_017314912.1 , NM_029924.2 , XM_030246624.1 , XM_030246629.1 , XM_017314911.2 , XM_017314909.1 , XM_017314911.1
    Accession Version NM_029924.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-11
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845521.9, AL845298.5 and AK173176.1. On Sep 4, 2008 this sequence version replaced NM_029924.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). This variant has an inferred 5' UTR exon combination based on the mRNA AK155574.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## CDS exon combination :: AK173176.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_084200.1
    CDS1259..6445
    Translation

    Target ORF information:

    RefSeq Version NM_029924.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_029924.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17187
    Clone ID Related Accession (Same CDS sequence) XM_030246635.1 , XM_017314915.1 , NM_001290656.1
    Accession Version XM_017314915.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GTGATATATC ATCCATAAAC
    AATTCATTGA ATAACCATCA ACTGACTCAT CTACAGTCGC TACTAAACAG CAATCAGATG
    TTTCCTCCAA ATCAGCAGCC GCAGCAACAC CTTCTCCAGG GACACCAGAA TCTCCAGGCC
    TTCCAAGGAC AACCCACAGT CCCTTGCCCA GCTAACAATA ACCCCATGGC TTGTCTGTTT
    CAGAACTTCC AGGTGAGGAT GCAAGGAGAT GCAGCTCTTC TAAATAAAAG AATAAGCACT
    CAGCCTGGGC TCACAACACT TCCTGAAAAC CCAAACCTTG CACTTCCACA TTTCCAAGAT
    ACACCCTGTG AGCTGCAGCC AAGGATTGAC CTTGGTCAGC CTATGAAGGA CGGCCTCGTT
    ATGGGTGGCC AAGGAGATGC CTCCGTAGAC GCCATTTACA AAGCAGTTGT GGATGCAGCC
    AGCAAAGGAA TGCAGGTTGT CATCACCACT GCTGTCAACA GCACGACTCA AATCAGCCCT
    ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC ACTGCCTCCA TTGGTGACCC ATTAAATCTG
    TCCAGTGCTG TGAGCGCTGT CATCCACGGG CGGAACATGG GAGGAGTGGA TCATGATGGC
    AGGCTGAGGA ATGCGAGAGG GGCACGGCTA CCCAAGAACA TAGACCATGG GAAGAACTCC
    AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG TCAGCGAGCT GCCACACATC CAGAAAACAG
    TGGGATGGGG AGCAAAGTCC CAGAGGGGAG CGCAACAGGT GGAAGTATGA GGAGTTTTTA
    GATCATCCAG GCCATATCCA CAGTAGTCCT TGCCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TAGCTGGGGA ACAGCACCCG ATACTGTTAC CACCAAGAAA CTGTCAAGGT
    GATAAGATTC TGGAGGAAAA TTTCAGGTAT AATAATTACA AAAGAACTAT GATGAGTTTT
    AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA TGTACACACA TTAACGGAAA CAGACCTCGT
    CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC ACTGCAAAAC AAGACCTAGT TCTAGAGGGG
    CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AACCATTAAT GGGTGTGTGC CTAGTCCCTC AGATGCTAAA
    AGCATTAGTA GTGAAGACGA CCTACGGAAT CCAGACTCCC CCTCCTCACA TGAGTTGATA
    CACTATAGAC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGGAAATT CATAAGAGAA GACGACGTTC ACAATTCATG CCAACAAAGC
    CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG TTGAAGACTC TCACAGAAGG TTTAGAAGCC
    TACAGCCGCG TCCGGAAAAG GAGCAGAAAA AGTGGGAAAC TAAATAACCA TTTAGAAGCT
    GCTATTCACG AGGCCATGAG CGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCA
    CAGGGTGACA GACAAATGAG GCCCCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170404.1
    CDS1270..5766
    Translation

    Target ORF information:

    RefSeq Version XM_017314915.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GTGATATATC ATCCATAAAC
    AATTCATTGA ATAACCATCA ACTGACTCAT CTACAGTCGC TACTAAACAG CAATCAGATG
    TTTCCTCCAA ATCAGCAGCC GCAGCAACAC CTTCTCCAGG GACACCAGAA TCTCCAGGCC
    TTCCAAGGAC AACCCACAGT CCCTTGCCCA GCTAACAATA ACCCCATGGC TTGTCTGTTT
    CAGAACTTCC AGGTGAGGAT GCAAGGAGAT GCAGCTCTTC TAAATAAAAG AATAAGCACT
    CAGCCTGGGC TCACAACACT TCCTGAAAAC CCAAACCTTG CACTTCCACA TTTCCAAGAT
    ACACCCTGTG AGCTGCAGCC AAGGATTGAC CTTGGTCAGC CTATGAAGGA CGGCCTCGTT
    ATGGGTGGCC AAGGAGATGC CTCCGTAGAC GCCATTTACA AAGCAGTTGT GGATGCAGCC
    AGCAAAGGAA TGCAGGTTGT CATCACCACT GCTGTCAACA GCACGACTCA AATCAGCCCT
    ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC ACTGCCTCCA TTGGTGACCC ATTAAATCTG
    TCCAGTGCTG TGAGCGCTGT CATCCACGGG CGGAACATGG GAGGAGTGGA TCATGATGGC
    AGGCTGAGGA ATGCGAGAGG GGCACGGCTA CCCAAGAACA TAGACCATGG GAAGAACTCC
    AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG TCAGCGAGCT GCCACACATC CAGAAAACAG
    TGGGATGGGG AGCAAAGTCC CAGAGGGGAG CGCAACAGGT GGAAGTATGA GGAGTTTTTA
    GATCATCCAG GCCATATCCA CAGTAGTCCT TGCCACGAAA GGCCCAACAA TGTCTCTACA
    CTGCCATTTC TAGCTGGGGA ACAGCACCCG ATACTGTTAC CACCAAGAAA CTGTCAAGGT
    GATAAGATTC TGGAGGAAAA TTTCAGGTAT AATAATTACA AAAGAACTAT GATGAGTTTT
    AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA TGTACACACA TTAACGGAAA CAGACCTCGT
    CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC ACTGCAAAAC AAGACCTAGT TCTAGAGGGG
    CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA AGTTCTCTGG TCAAAGACTA CATCCATTAC
    AATGGAGACT TTAATGCCAA AACCATTAAT GGGTGTGTGC CTAGTCCCTC AGATGCTAAA
    AGCATTAGTA GTGAAGACGA CCTACGGAAT CCAGACTCCC CCTCCTCACA TGAGTTGATA
    CACTATAGAC CAAGGACGTT CAACGTTGGC GACTTGGTCT GGGGCCAAAT CAAAGGACTG
    ACTTCCTGGC CTGGGAAATT CATAAGAGAA GACGACGTTC ACAATTCATG CCAACAAAGC
    CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG TTGAAGACTC TCACAGAAGG TTTAGAAGCC
    TACAGCCGCG TCCGGAAAAG GAGCAGAAAA AGTGGGAAAC TAAATAACCA TTTAGAAGCT
    GCTATTCACG AGGCCATGAG CGAACTGGAC AAAATGTCTG GGACTGTTCA CCAAATCCCA
    CAGGGTGACA GACAAATGAG GCCCCCCAAA CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21530
    Clone ID Related Accession (Same CDS sequence) XM_030246630.1 , XM_017314910.1 , XM_030246625.1 , XM_030246626.1 , XM_030246628.1 , XM_017314912.1 , NM_029924.2 , XM_030246624.1 , XM_030246629.1 , XM_017314911.2 , XM_017314909.1 , XM_017314911.1
    Accession Version XM_017314910.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170399.1
    CDS1460..6646
    Translation

    Target ORF information:

    RefSeq Version XM_017314910.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314910.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21530
    Clone ID Related Accession (Same CDS sequence) XM_030246630.1 , XM_017314910.1 , XM_030246625.1 , XM_030246626.1 , XM_030246628.1 , XM_017314912.1 , NM_029924.2 , XM_030246624.1 , XM_030246629.1 , XM_017314911.2 , XM_017314909.1 , XM_017314911.1
    Accession Version XM_017314912.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170401.1
    CDS1269..6455
    Translation

    Target ORF information:

    RefSeq Version XM_017314912.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314912.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21530
    Clone ID Related Accession (Same CDS sequence) XM_030246630.1 , XM_017314910.1 , XM_030246625.1 , XM_030246626.1 , XM_030246628.1 , XM_017314912.1 , NM_029924.2 , XM_030246624.1 , XM_030246629.1 , XM_017314911.2 , XM_017314909.1 , XM_017314911.1
    Accession Version XM_017314909.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170398.1
    CDS1374..6560
    Translation

    Target ORF information:

    RefSeq Version XM_017314909.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314909.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21530
    Clone ID Related Accession (Same CDS sequence) XM_030246630.1 , XM_017314910.1 , XM_030246625.1 , XM_030246626.1 , XM_030246628.1 , XM_017314912.1 , NM_029924.2 , XM_030246624.1 , XM_030246629.1 , XM_017314911.2 , XM_017314909.1 , XM_017314911.1
    Accession Version XM_017314911.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170400.1
    CDS1232..6418
    Translation

    Target ORF information:

    RefSeq Version XM_017314911.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314911.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGGTGGA GCCCGAGAAG
    TTGAAGACTC TCACAGAAGG TTTAGAAGCC TACAGCCGCG TCCGGAAAAG GAGCAGAAAA
    AGTGGGAAAC TAAATAACCA TTTAGAAGCT GCTATTCACG AGGCCATGAG CGAACTGGAC
    AAAATGTCTG GGACTGTTCA CCAAATCCCA CAGGGTGACA GACAAATGAG GCCCCCCAAA
    CCCAAGAGGA GGAAGATCTC CAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29239
    Clone ID Related Accession (Same CDS sequence) XM_006497604.2 , XM_006497604.3
    Accession Version XM_006497604.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497604.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCAAGTGT GGCTTGGAAT GTCCTCTTAT TCTTCCCAAG ATGCAACTCC AGTAGTACCT 
    TCACGGGCGG CAACTCCAAG ATCAGTAAGA AACAAGTCTC ATGAAGGAAT TACAAATTCT
    GTAATGCCAG AATGTAAGAA TCCTTTCAAG TTAATGACTG GATCATCAAA TGCCATGGGA
    AGGCTATACA TGCAAGACCT GCCTGGAAGC CAGCAGCAGG AGCTCCACCC TGTCTACCCC
    CGCCAGAGAT TGGGCAGTAG TGAGCATGGA CAGAAATCTC CATTTCGTGG CAGCCATGGA
    GGCCTGCCTA GCCCAGCATC ATCAGGGTCT CAGATCTATG GAGATGGCTC AATCTCTCCA
    AGGACTGACC CGCTCGGAAG CCCTGATGTC TTCACAAGAA ATAACCCTGG TTTTCATGGA
    GCTCCCAATT CCAGCCCTAT TCACCTAAAT AGGACTCCTC TTTCTCCACC TTCAGTAATG
    CTACATGGTT CTCCCGTACA GTCATCCTGT GCAATGGCTG GAAGAACTAA TATACCTCTT
    TCCCCAACCT TGACTACAAA GAGTCCAGTA ATGAAAAAAC CAATGTGTAA TTTTTCAACT
    AATATGGAAA TACCACGAGC AATGTTCCAC CACAAACCAC CCCAAGGCCC ACCTCCCCCT
    CCTCCACCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CTGAGAAAGA TCCACTTGGC
    ATTCTTGACC CTATTCCTAG TAAACCAGTG AATCAGAACC CTATTATCAT TAATCCAACC
    AGTTTCCATT CAAATGTCCA CTCTCAGGTA CCTGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTTGTTCCTT TGCCAAGTAA TCTCCCTTTG CCAACTGTAA AACCTGGTCA TATGAATCAT
    GGGAGTCATG TACAAAGAAT TCAGCATTCA GCTTCAACCT CCCTGTCCCC TTCTCCAGTG
    ACATCCCCAG TGCACATGAT GGGGACTGGA ATTGGAAGGA TTGAGGCATC GCCCCAAAGA
    TCACGCTCAT CTTCCACATC ATCAGATCAT GGAAATTTCA TGATGCCACC TGTAGGACCC
    CAGGCCACTT GTAGTGGCAT AAAGGTTCCA CCTAGGTCAC CAAGGTCAAC AATAGGTTCC
    CCAAGGCCAT CAATGCCATC AAGCCCTTCT ACCAAGTCTG ATGGACATCA TCAGTACAAG
    GATATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAC TCAATACCCC AAGCAGTGCA
    GCTTTTCCTA CTGCACCTGC CGGAAATGGT TCTGTAAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CCTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAACA AACTTGCTGG TAACAACAGT
    AGCAGCAGTA ACAATTCTGG AGCTGTTGCC AGCAGCGGCA ACACTGAAGG ACACAGCACT
    TTAAACACCA TGTTCCCTCC AACTGCCAAC ATGCTTCTCC CAACAGGTGA AGGTCAAAGT
    GGTCGAGCAG CACTAAGAGA TAAGTTGATG TCTCAGCAAA AAGACTCATT ACGGAAAAGA
    AAGCAGCCAC CCACCACAGT GTTGAGTTTG CTCAGACAGT CCCAAATGGA TAGTTCTGCA
    GCTCCTAAAC CTGGACCTGA CTTGCTAAGG AAGCATGGGC AGGGGTCATT TCCCATCAGT
    TCAATGTCTC AGTTACTACA GTCTATGAGT TGTCAAAGCT CTCACTTGAG TAGCAATAGT
    ACCCCGGGTT GTGGGGGATC AAATACTGCT TTGCCTTGCT CTGCTAACCA GCTGCATTTT
    CCAGATCCTA ATATGAATTC CACTGTTCTT CAGAATTCAC TGACACAGAG CATACCTTTA
    AGAGGGGAAG CCGTGCACTG CCACAATGCA AACACTAACT TTGTTCACAG TAACAGTCCA
    GTCCCCAACC ACCATCTTGC AGGTTTAATA AATCAGATTC AGGCTAGCGG GAACTGTGGG
    ATGCTCAGTC AGTCGGGCAT GGCTTTAGGA AACTCATTAC ATCCCAATCC ACCTCAGTCA
    AGAATTTCAA CGTCCTCCAC TCCAGTGATA CCAAACAGCA TTGTTAGCAG CTATAATCAA
    ACAAGTTCTG AAGCAGGTGG TTCAAGCCTG CCGTCCTCCA TAGCTATAGC TGGCAGCAAC
    CACCCTGCCA TCACAAAGAC AACATCTGTT CTCCAAGATG GTGTCATAGT CACCACCGCA
    GCTGGAAACC CACTGCAGAG TCAGCTGCCC ATTGGGAGTG ACTTTCCTTT TGTTGGCCAA
    GAGCATGCCC TTCATTTTCC ATCCAACAGC ACCGCAAACA ACCATCTTCC ACACCCCTTG
    AACCCCAGCC TCCTCAGCTC TCTACCTATC TCTTTGCCAG TGAATCAACA GCATCTCCTA
    AACCAGAATC TATTAAATAT CCTCCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGATCAAC
    CTCCACCCTT TAGGTTTTCT CAACCCGAAT GTAAACGCTG CTTTAGCTTT CCTCTCCGGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTC CACTTTCAGC TCTTAGCAGC CCTGCTTCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTGCCGTCTT TCAATCTGAC CATCTCAGAT
    CTTTTGCAAC CGCAAAATAC CCCTTTACCC TTATTAACAC AGATGACAGC CCCACCAGAC
    CGTTTGCCAA GCAATCAGTC AGACAACAGC CGAGCTGATA CCCTTTTAGG CAGCCCCCTG
    GGAAACCCTT TAGCAGGCAG TGACACTACT TTTAACCCCC TGCTCCTCCC AGCTGTCACT
    GGGGCCTCAG GATTAATGGC CTTGAATCCC CAGCTGTTGG GGGGTGTCCT GAACTCGGCA
    TCGGCCAACA CCGCTATTCA TCCAGAGGTT TCCATAGCAA CCTCCTCCCA GGCAACCACT
    ACCACAACCA CTACATCATC AGCAGTGGCA GCACTGACTG TCTCAACACT TGGTGGGACA
    GCAGTGGTGT CAATGGCAGA AACATTGCTG AATATATCTA ATAATGCTGG GAATCCACCT
    GGTCCAGCTA AACTCAACAG TAACTCTATG GTGCCACAGC TACTTAACCC TCTACTGGGG
    ACAGGTCTGC TTGGTGATAT ATCATCCATA AACAATTCAT TGAATAACCA TCAACTGACT
    CATCTACAGT CGCTACTAAA CAGCAATCAG ATGTTTCCTC CAAATCAGCA GCCGCAGCAA
    CACCTTCTCC AGGGACACCA GAATCTCCAG GCCTTCCAAG GACAACCCAC AGTCCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAACT TCCAGGTGAG GATGCAAGGA
    GATGCAGCTC TTCTAAATAA AAGAATAAGC ACTCAGCCTG GGCTCACAAC ACTTCCTGAA
    AACCCAAACC TTGCACTTCC ACATTTCCAA GATACACCCT GTGAGCTGCA GCCAAGGATT
    GACCTTGGTC AGCCTATGAA GGACGGCCTC GTTATGGGTG GCCAAGGAGA TGCCTCCGTA
    GACGCCATTT ACAAAGCAGT TGTGGATGCA GCCAGCAAAG GAATGCAGGT TGTCATCACC
    ACTGCTGTCA ACAGCACGAC TCAAATCAGC CCTATCCCAG CCCTGAGCGC CATGAGTGCC
    TTCACTGCCT CCATTGGTGA CCCATTAAAT CTGTCCAGTG CTGTGAGCGC TGTCATCCAC
    GGGCGGAACA TGGGAGGAGT GGATCATGAT GGCAGGCTGA GGAATGCGAG AGGGGCACGG
    CTACCCAAGA ACATAGACCA TGGGAAGAAC TCCAGCGAAG GAGACGGGTT TGAGTGTTTC
    AAGTCAGCGA GCTGCCACAC ATCCAGAAAA CAGTGGGATG GGGAGCAAAG TCCCAGAGGG
    GAGCGCAACA GGTGGAAGTA TGAGGAGTTT TTAGATCATC CAGGCCATAT CCACAGTAGT
    CCTTGCCACG AAAGGCCCAA CAATGTCTCT ACACTGCCAT TTCTAGCTGG GGAACAGCAC
    CCGATACTGT TACCACCAAG AAACTGTCAA GGTGATAAGA TTCTGGAGGA AAATTTCAGG
    TATAATAATT ACAAAAGAAC TATGATGAGT TTTAAAGAGA GACTAGAGAG TACTGTGGAG
    AGATGTACAC ACATTAACGG AAACAGACCT CGTCAGAGTC GGGGCTTTGG AGAGCTGCTA
    GGCACTGCAA AACAAGACCT AGTTCTAGAG GGGCAGTCTC CGGGTTCTTC AAATAGTTTG
    GAAAGTTCTC TGGTCAAAGA CTACATCCAT TACAATGGAG ACTTTAATGC CAAAACCATT
    AATGGGTGTG TGCCTAGTCC CTCAGATGCT AAAAGCATTA GTAGTGAAGA CGACCTACGG
    AATCCAGACT CCCCCTCCTC ACATGAGTTG ATACACTATA GACCAAGGAC GTTCAACGTT
    GGCGACTTGG TCTGGGGCCA AATCAAAGGA CTGACTTCCT GGCCTGGGAA ATTCATAAGA
    GAAGACGACG TTCACAATTC ATGCCAACAA AGCCCCGAGG AAGGGAAGGT GGAGCCCGAG
    AAGTTGAAGA CTCTCACAGA AGGTTTAGAA GCCTACAGCC GCGTCCGGAA AAGGAGCAGA
    AAAAGTGGGA AACTAAATAA CCATTTAGAA GCTGCTATTC ACGAGGCCAT GAGCGAACTG
    GACAAAATGT CTGGGACTGT TCACCAAATC CCACAGGGTG ACAGACAAAT GAGGCCCCCC
    AAACCCAAGA GGAGGAAGAT CTCCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497667.1
    CDS154..4983
    Translation

    Target ORF information:

    RefSeq Version XM_006497604.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006497604.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCAAGTGT GGCTTGGAAT GTCCTCTTAT TCTTCCCAAG ATGCAACTCC AGTAGTACCT 
    TCACGGGCGG CAACTCCAAG ATCAGTAAGA AACAAGTCTC ATGAAGGAAT TACAAATTCT
    GTAATGCCAG AATGTAAGAA TCCTTTCAAG TTAATGACTG GATCATCAAA TGCCATGGGA
    AGGCTATACA TGCAAGACCT GCCTGGAAGC CAGCAGCAGG AGCTCCACCC TGTCTACCCC
    CGCCAGAGAT TGGGCAGTAG TGAGCATGGA CAGAAATCTC CATTTCGTGG CAGCCATGGA
    GGCCTGCCTA GCCCAGCATC ATCAGGGTCT CAGATCTATG GAGATGGCTC AATCTCTCCA
    AGGACTGACC CGCTCGGAAG CCCTGATGTC TTCACAAGAA ATAACCCTGG TTTTCATGGA
    GCTCCCAATT CCAGCCCTAT TCACCTAAAT AGGACTCCTC TTTCTCCACC TTCAGTAATG
    CTACATGGTT CTCCCGTACA GTCATCCTGT GCAATGGCTG GAAGAACTAA TATACCTCTT
    TCCCCAACCT TGACTACAAA GAGTCCAGTA ATGAAAAAAC CAATGTGTAA TTTTTCAACT
    AATATGGAAA TACCACGAGC AATGTTCCAC CACAAACCAC CCCAAGGCCC ACCTCCCCCT
    CCTCCACCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CTGAGAAAGA TCCACTTGGC
    ATTCTTGACC CTATTCCTAG TAAACCAGTG AATCAGAACC CTATTATCAT TAATCCAACC
    AGTTTCCATT CAAATGTCCA CTCTCAGGTA CCTGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTTGTTCCTT TGCCAAGTAA TCTCCCTTTG CCAACTGTAA AACCTGGTCA TATGAATCAT
    GGGAGTCATG TACAAAGAAT TCAGCATTCA GCTTCAACCT CCCTGTCCCC TTCTCCAGTG
    ACATCCCCAG TGCACATGAT GGGGACTGGA ATTGGAAGGA TTGAGGCATC GCCCCAAAGA
    TCACGCTCAT CTTCCACATC ATCAGATCAT GGAAATTTCA TGATGCCACC TGTAGGACCC
    CAGGCCACTT GTAGTGGCAT AAAGGTTCCA CCTAGGTCAC CAAGGTCAAC AATAGGTTCC
    CCAAGGCCAT CAATGCCATC AAGCCCTTCT ACCAAGTCTG ATGGACATCA TCAGTACAAG
    GATATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAC TCAATACCCC AAGCAGTGCA
    GCTTTTCCTA CTGCACCTGC CGGAAATGGT TCTGTAAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CCTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAACA AACTTGCTGG TAACAACAGT
    AGCAGCAGTA ACAATTCTGG AGCTGTTGCC AGCAGCGGCA ACACTGAAGG ACACAGCACT
    TTAAACACCA TGTTCCCTCC AACTGCCAAC ATGCTTCTCC CAACAGGTGA AGGTCAAAGT
    GGTCGAGCAG CACTAAGAGA TAAGTTGATG TCTCAGCAAA AAGACTCATT ACGGAAAAGA
    AAGCAGCCAC CCACCACAGT GTTGAGTTTG CTCAGACAGT CCCAAATGGA TAGTTCTGCA
    GCTCCTAAAC CTGGACCTGA CTTGCTAAGG AAGCATGGGC AGGGGTCATT TCCCATCAGT
    TCAATGTCTC AGTTACTACA GTCTATGAGT TGTCAAAGCT CTCACTTGAG TAGCAATAGT
    ACCCCGGGTT GTGGGGGATC AAATACTGCT TTGCCTTGCT CTGCTAACCA GCTGCATTTT
    CCAGATCCTA ATATGAATTC CACTGTTCTT CAGAATTCAC TGACACAGAG CATACCTTTA
    AGAGGGGAAG CCGTGCACTG CCACAATGCA AACACTAACT TTGTTCACAG TAACAGTCCA
    GTCCCCAACC ACCATCTTGC AGGTTTAATA AATCAGATTC AGGCTAGCGG GAACTGTGGG
    ATGCTCAGTC AGTCGGGCAT GGCTTTAGGA AACTCATTAC ATCCCAATCC ACCTCAGTCA
    AGAATTTCAA CGTCCTCCAC TCCAGTGATA CCAAACAGCA TTGTTAGCAG CTATAATCAA
    ACAAGTTCTG AAGCAGGTGG TTCAAGCCTG CCGTCCTCCA TAGCTATAGC TGGCAGCAAC
    CACCCTGCCA TCACAAAGAC AACATCTGTT CTCCAAGATG GTGTCATAGT CACCACCGCA
    GCTGGAAACC CACTGCAGAG TCAGCTGCCC ATTGGGAGTG ACTTTCCTTT TGTTGGCCAA
    GAGCATGCCC TTCATTTTCC ATCCAACAGC ACCGCAAACA ACCATCTTCC ACACCCCTTG
    AACCCCAGCC TCCTCAGCTC TCTACCTATC TCTTTGCCAG TGAATCAACA GCATCTCCTA
    AACCAGAATC TATTAAATAT CCTCCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGATCAAC
    CTCCACCCTT TAGGTTTTCT CAACCCGAAT GTAAACGCTG CTTTAGCTTT CCTCTCCGGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTC CACTTTCAGC TCTTAGCAGC CCTGCTTCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTGCCGTCTT TCAATCTGAC CATCTCAGAT
    CTTTTGCAAC CGCAAAATAC CCCTTTACCC TTATTAACAC AGATGACAGC CCCACCAGAC
    CGTTTGCCAA GCAATCAGTC AGACAACAGC CGAGCTGATA CCCTTTTAGG CAGCCCCCTG
    GGAAACCCTT TAGCAGGCAG TGACACTACT TTTAACCCCC TGCTCCTCCC AGCTGTCACT
    GGGGCCTCAG GATTAATGGC CTTGAATCCC CAGCTGTTGG GGGGTGTCCT GAACTCGGCA
    TCGGCCAACA CCGCTATTCA TCCAGAGGTT TCCATAGCAA CCTCCTCCCA GGCAACCACT
    ACCACAACCA CTACATCATC AGCAGTGGCA GCACTGACTG TCTCAACACT TGGTGGGACA
    GCAGTGGTGT CAATGGCAGA AACATTGCTG AATATATCTA ATAATGCTGG GAATCCACCT
    GGTCCAGCTA AACTCAACAG TAACTCTATG GTGCCACAGC TACTTAACCC TCTACTGGGG
    ACAGGTCTGC TTGGTGATAT ATCATCCATA AACAATTCAT TGAATAACCA TCAACTGACT
    CATCTACAGT CGCTACTAAA CAGCAATCAG ATGTTTCCTC CAAATCAGCA GCCGCAGCAA
    CACCTTCTCC AGGGACACCA GAATCTCCAG GCCTTCCAAG GACAACCCAC AGTCCCTTGC
    CCAGCTAACA ATAACCCCAT GGCTTGTCTG TTTCAGAACT TCCAGGTGAG GATGCAAGGA
    GATGCAGCTC TTCTAAATAA AAGAATAAGC ACTCAGCCTG GGCTCACAAC ACTTCCTGAA
    AACCCAAACC TTGCACTTCC ACATTTCCAA GATACACCCT GTGAGCTGCA GCCAAGGATT
    GACCTTGGTC AGCCTATGAA GGACGGCCTC GTTATGGGTG GCCAAGGAGA TGCCTCCGTA
    GACGCCATTT ACAAAGCAGT TGTGGATGCA GCCAGCAAAG GAATGCAGGT TGTCATCACC
    ACTGCTGTCA ACAGCACGAC TCAAATCAGC CCTATCCCAG CCCTGAGCGC CATGAGTGCC
    TTCACTGCCT CCATTGGTGA CCCATTAAAT CTGTCCAGTG CTGTGAGCGC TGTCATCCAC
    GGGCGGAACA TGGGAGGAGT GGATCATGAT GGCAGGCTGA GGAATGCGAG AGGGGCACGG
    CTACCCAAGA ACATAGACCA TGGGAAGAAC TCCAGCGAAG GAGACGGGTT TGAGTGTTTC
    AAGTCAGCGA GCTGCCACAC ATCCAGAAAA CAGTGGGATG GGGAGCAAAG TCCCAGAGGG
    GAGCGCAACA GGTGGAAGTA TGAGGAGTTT TTAGATCATC CAGGCCATAT CCACAGTAGT
    CCTTGCCACG AAAGGCCCAA CAATGTCTCT ACACTGCCAT TTCTAGCTGG GGAACAGCAC
    CCGATACTGT TACCACCAAG AAACTGTCAA GGTGATAAGA TTCTGGAGGA AAATTTCAGG
    TATAATAATT ACAAAAGAAC TATGATGAGT TTTAAAGAGA GACTAGAGAG TACTGTGGAG
    AGATGTACAC ACATTAACGG AAACAGACCT CGTCAGAGTC GGGGCTTTGG AGAGCTGCTA
    GGCACTGCAA AACAAGACCT AGTTCTAGAG GGGCAGTCTC CGGGTTCTTC AAATAGTTTG
    GAAAGTTCTC TGGTCAAAGA CTACATCCAT TACAATGGAG ACTTTAATGC CAAAACCATT
    AATGGGTGTG TGCCTAGTCC CTCAGATGCT AAAAGCATTA GTAGTGAAGA CGACCTACGG
    AATCCAGACT CCCCCTCCTC ACATGAGTTG ATACACTATA GACCAAGGAC GTTCAACGTT
    GGCGACTTGG TCTGGGGCCA AATCAAAGGA CTGACTTCCT GGCCTGGGAA ATTCATAAGA
    GAAGACGACG TTCACAATTC ATGCCAACAA AGCCCCGAGG AAGGGAAGGT GGAGCCCGAG
    AAGTTGAAGA CTCTCACAGA AGGTTTAGAA GCCTACAGCC GCGTCCGGAA AAGGAGCAGA
    AAAAGTGGGA AACTAAATAA CCATTTAGAA GCTGCTATTC ACGAGGCCAT GAGCGAACTG
    GACAAAATGT CTGGGACTGT TCACCAAATC CCACAGGGTG ACAGACAAAT GAGGCCCCCC
    AAACCCAAGA GGAGGAAGAT CTCCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72275
    Clone ID Related Accession (Same CDS sequence) XM_017314914.1
    Accession Version XM_017314914.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product methyl-CpG-binding domain protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGATTCT TCATGACTCA
    TTGAGGTCTC ACAAGCTTCT GGAACATAAA ATGAAGAGGG GATGGTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170403.1
    CDS1277..6307
    Translation

    Target ORF information:

    RefSeq Version XM_017314914.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATGGAG GCAAAGAGTG TGACGGAGGA GACAAGGAAG GAGGTCTTGC AGCTATCCAG 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAC CATAATGGCG TGCTGTACAT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGACCAGGTT AAGACATACC TGCTCACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC AGAAGATGTT AAGGCAGATG ATGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAGATCAT TGCAGTGGCC ACACTCCATC AAAGCATGGA GGCCCCGCAT
    CCTTCTCTGG TGCTCACCAG TCCTGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCGGCAA CTCCAAGATC AGTAAGAAAC AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCAGAAT GTAAGAATCC TTTCAAGTTA ATGACTGGAT CATCAAATGC CATGGGAAGG
    CTATACATGC AAGACCTGCC TGGAAGCCAG CAGCAGGAGC TCCACCCTGT CTACCCCCGC
    CAGAGATTGG GCAGTAGTGA GCATGGACAG AAATCTCCAT TTCGTGGCAG CCATGGAGGC
    CTGCCTAGCC CAGCATCATC AGGGTCTCAG ATCTATGGAG ATGGCTCAAT CTCTCCAAGG
    ACTGACCCGC TCGGAAGCCC TGATGTCTTC ACAAGAAATA ACCCTGGTTT TCATGGAGCT
    CCCAATTCCA GCCCTATTCA CCTAAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CCGTACAGTC ATCCTGTGCA ATGGCTGGAA GAACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTA TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACTGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATGTAC AAAGAATTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTGTA GTGGCATAAA GGTTCCACCT AGGTCACCAA GGTCAACAAT AGGTTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCTGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTCA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CACCTGCCGG AAATGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAACA ATTCTGGAGC TGTTGCCAGC AGCGGCAACA CTGAAGGACA CAGCACTTTA
    AACACCATGT TCCCTCCAAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG TCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GTTGATGTCT CAGCAAAAAG ACTCATTACG GAAAAGAAAG
    CAGCCACCCA CCACAGTGTT GAGTTTGCTC AGACAGTCCC AAATGGATAG TTCTGCAGCT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CATGGGCAGG GGTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGATCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTCCA
    GATCCTAATA TGAATTCCAC TGTTCTTCAG AATTCACTGA CACAGAGCAT ACCTTTAAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCCAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAC TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGTGGTTC AAGCCTGCCG TCCTCCATAG CTATAGCTGG CAGCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTC CAAGATGGTG TCATAGTCAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCCATT GGGAGTGACT TTCCTTTTGT TGGCCAAGAG
    CATGCCCTTC ATTTTCCATC CAACAGCACC GCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGCTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTCCT CTCCGGTGAC
    GTGGATGGGC AGGTATTACA GCCTGTCCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCGTCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACCGC AAAATACCCC TTTACCCTTA TTAACACAGA TGACAGCCCC ACCAGACCGT
    TTGCCAAGCA ATCAGTCAGA CAACAGCCGA GCTGATACCC TTTTAGGCAG CCCCCTGGGA
    AACCCTTTAG CAGGCAGTGA CACTACTTTT AACCCCCTGC TCCTCCCAGC TGTCACTGGG
    GCCTCAGGAT TAATGGCCTT GAATCCCCAG CTGTTGGGGG GTGTCCTGAA CTCGGCATCG
    GCCAACACCG CTATTCATCC AGAGGTTTCC ATAGCAACCT CCTCCCAGGC AACCACTACC
    ACAACCACTA CATCATCAGC AGTGGCAGCA CTGACTGTCT CAACACTTGG TGGGACAGCA
    GTGGTGTCAA TGGCAGAAAC ATTGCTGAAT ATATCTAATA ATGCTGGGAA TCCACCTGGT
    CCAGCTAAAC TCAACAGTAA CTCTATGGTG CCACAGCTAC TTAACCCTCT ACTGGGGACA
    GGTCTGCTTG GTGATATATC ATCCATAAAC AATTCATTGA ATAACCATCA ACTGACTCAT
    CTACAGTCGC TACTAAACAG CAATCAGATG TTTCCTCCAA ATCAGCAGCC GCAGCAACAC
    CTTCTCCAGG GACACCAGAA TCTCCAGGCC TTCCAAGGAC AACCCACAGT CCCTTGCCCA
    GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTCC AGGTGAGGAT GCAAGGAGAT
    GCAGCTCTTC TAAATAAAAG AATAAGCACT CAGCCTGGGC TCACAACACT TCCTGAAAAC
    CCAAACCTTG CACTTCCACA TTTCCAAGAT ACACCCTGTG AGCTGCAGCC AAGGATTGAC
    CTTGGTCAGC CTATGAAGGA CGGCCTCGTT ATGGGTGGCC AAGGAGATGC CTCCGTAGAC
    GCCATTTACA AAGCAGTTGT GGATGCAGCC AGCAAAGGAA TGCAGGTTGT CATCACCACT
    GCTGTCAACA GCACGACTCA AATCAGCCCT ATCCCAGCCC TGAGCGCCAT GAGTGCCTTC
    ACTGCCTCCA TTGGTGACCC ATTAAATCTG TCCAGTGCTG TGAGCGCTGT CATCCACGGG
    CGGAACATGG GAGGAGTGGA TCATGATGGC AGGCTGAGGA ATGCGAGAGG GGCACGGCTA
    CCCAAGAACA TAGACCATGG GAAGAACTCC AGCGAAGGAG ACGGGTTTGA GTGTTTCAAG
    TCAGCGAGCT GCCACACATC CAGAAAACAG TGGGATGGGG AGCAAAGTCC CAGAGGGGAG
    CGCAACAGGT GGAAGTATGA GGAGTTTTTA GATCATCCAG GCCATATCCA CAGTAGTCCT
    TGCCACGAAA GGCCCAACAA TGTCTCTACA CTGCCATTTC TAGCTGGGGA ACAGCACCCG
    ATACTGTTAC CACCAAGAAA CTGTCAAGGT GATAAGATTC TGGAGGAAAA TTTCAGGTAT
    AATAATTACA AAAGAACTAT GATGAGTTTT AAAGAGAGAC TAGAGAGTAC TGTGGAGAGA
    TGTACACACA TTAACGGAAA CAGACCTCGT CAGAGTCGGG GCTTTGGAGA GCTGCTAGGC
    ACTGCAAAAC AAGACCTAGT TCTAGAGGGG CAGTCTCCGG GTTCTTCAAA TAGTTTGGAA
    AGTTCTCTGG TCAAAGACTA CATCCATTAC AATGGAGACT TTAATGCCAA AACCATTAAT
    GGGTGTGTGC CTAGTCCCTC AGATGCTAAA AGCATTAGTA GTGAAGACGA CCTACGGAAT
    CCAGACTCCC CCTCCTCACA TGAGTTGATA CACTATAGAC CAAGGACGTT CAACGTTGGC
    GACTTGGTCT GGGGCCAAAT CAAAGGACTG ACTTCCTGGC CTGGGAAATT CATAAGAGAA
    GACGACGTTC ACAATTCATG CCAACAAAGC CCCGAGGAAG GGAAGATTCT TCATGACTCA
    TTGAGGTCTC ACAAGCTTCT GGAACATAAA ATGAAGAGGG GATGGTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.