MBD5 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017004482.1	XP_016859971.1	methyl-CpG-binding domain protein 5 isoform X2
XM_017004484.1	XP_016859973.1	methyl-CpG-binding domain protein 5 isoform X4
XM_024452988.1	XP_024308756.1	methyl-CpG-binding domain protein 5 isoform X3
XM_017004483.1	XP_016859972.1	methyl-CpG-binding domain protein 5 isoform X3
NM_018328.4	NP_060798.2	methyl-CpG-binding domain protein 5
XM_024452990.1	XP_024308758.1	methyl-CpG-binding domain protein 5 isoform X5
XM_017004485.1	XP_016859974.1	methyl-CpG-binding domain protein 5 isoform X5
XM_011511470.2	XP_011509772.1	methyl-CpG-binding domain protein 5 isoform X1
XM_024452987.1	XP_024308755.1	methyl-CpG-binding domain protein 5 isoform X2
XM_024452989.1	XP_024308757.1	methyl-CpG-binding domain protein 5 isoform X4
XM_011511472.2	XP_011509774.1	methyl-CpG-binding domain protein 5 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19437	NM_018328.4 Latest version!	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu36432	XM_017004482.1 Latest version!	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu36432	XM_024452987.1 Latest version!	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu69742	XM_011511470.2 Latest version!	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu69742	XM_011511472.2 Latest version!	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu69743	XM_017004483.1 Latest version!	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu69743	XM_024452988.1 Latest version!	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu69744	XM_024452989.1 Latest version!	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu69744	XM_017004484.1 Latest version!	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu69745	XM_017004485.1 Latest version!	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu69745	XM_024452990.1 Latest version!	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

MBD5 ( NM_018328.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060798.2 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), mRNA.

Price & Availability↑

CloneID	OHu19437
Clone ID Related Accession (Same CDS sequence)	NM_018328.4
Accession Version	NM_018328.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA240785.1, EF542797.1, AB040894.1 and CA449443.1. This sequence is a reference standard in the RefSeqGene project. On or before Aug 31, 2013 this sequence version replaced XM_005263713.1, NM_018328.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: EF542797.1, BC150264.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC 
CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG 
CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA 
ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA 
ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA 
CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA 
CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA 
GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG 
CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG 
AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC 
AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT 
TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT 
GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG 
CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC 
CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG 
CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA 
GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA 
GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA 
TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT 
TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT 
AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT 
GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA 
AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT 
GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG 
AAGGTGGAGC CCGAGAAGTT GAAGACACTA ACAGAAGGTT TGGAAGCCTA CAGCCGTGTC 
CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA AATAACCATT TAGAAGCTGC TATTCATGAG 
GCCATGAGTG AACTGGACAA AATGTCTGGG ACTGTACACC AAATCCCACA GGGTGACAGA 
CAAATGAGAC CCCCCAAACC CAAGAGGAGG AAGATCTCCA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060798.2
CDS	998..5482
Translation	MNGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRISTQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVIHGRNMGGVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSPRGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILEENFRYNNYKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLENSLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRVRKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	NM_018328.4
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018328.4

ORF Insert Sequence:

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC 
CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG 
CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA 
ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA 
ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA 
CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA 
CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA 
GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG 
CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG 
AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC 
AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT 
TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT 
GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG 
CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC 
CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG 
CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA 
GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA 
GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA 
TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT 
TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT 
AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT 
GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA 
AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT 
GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG 
AAGGTGGAGC CCGAGAAGTT GAAGACACTA ACAGAAGGTT TGGAAGCCTA CAGCCGTGTC 
CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA AATAACCATT TAGAAGCTGC TATTCATGAG 
GCCATGAGTG AACTGGACAA AATGTCTGGG ACTGTACACC AAATCCCACA GGGTGACAGA 
CAAATGAGAC CCCCCAAACC CAAGAGGAGG AAGATCTCCA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_017004482.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859971.1 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu36432
Clone ID Related Accession (Same CDS sequence)	XM_017004482.1 , XM_024452987.1
Accession Version	XM_017004482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT 
GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA 
ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC 
AAGAGGAGGA AGATCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859971.1
CDS	680..5863
Translation	MNGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSSDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSDNSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVNGASGLMTLNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRISTQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVIHGRNMGGVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSPRGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILEENFRYNNYKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLENSLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRVRKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	XM_017004482.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004482.1

ORF Insert Sequence:

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT 
GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA 
ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC 
AAGAGGAGGA AGATCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_024452987.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308755.1 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

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CloneID	OHu36432
Clone ID Related Accession (Same CDS sequence)	XM_017004482.1 , XM_024452987.1
Accession Version	XM_024452987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT 
GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA 
ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC 
AAGAGGAGGA AGATCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308755.1
CDS	313..5496
Translation	MNGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSSDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSDNSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVNGASGLMTLNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRISTQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVIHGRNMGGVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSPRGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILEENFRYNNYKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLENSLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRVRKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	XM_024452987.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452987.1

ORF Insert Sequence:

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT 
GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA 
ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC 
AAGAGGAGGA AGATCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_011511470.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509772.1 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

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CloneID	OHu69742
Clone ID Related Accession (Same CDS sequence)	XM_011511470.2 , XM_011511472.2
Accession Version	XM_011511470.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511470.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA 
GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTACACCAA 
ATCCCACAGG GTGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509772.1
CDS	1230..6452
Translation	MNGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSSDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSDNSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVNGASGLMTLNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRISTQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVIHGRNMGGVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSPRGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILEENFRYNNYKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLENSLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEAYSRVRKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	XM_011511470.2
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511470.2

ORF Insert Sequence:

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA 
GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTACACCAA 
ATCCCACAGG GTGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_011511472.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509774.1 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

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CloneID	OHu69742
Clone ID Related Accession (Same CDS sequence)	XM_011511470.2 , XM_011511472.2
Accession Version	XM_011511472.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511472.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA 
GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTACACCAA 
ATCCCACAGG GTGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509774.1
CDS	810..6032
Translation	MNGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSSDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSDNSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVNGASGLMTLNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRISTQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVIHGRNMGGVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSPRGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILEENFRYNNYKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLENSLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEAYSRVRKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	XM_011511472.2
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511472.2

ORF Insert Sequence:

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA 
GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTACACCAA 
ATCCCACAGG GTGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_024452988.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308756.1 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

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CloneID	OHu69743
Clone ID Related Accession (Same CDS sequence)	XM_024452988.1 , XM_017004483.1
Accession Version	XM_024452988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAACTGA GGGGAAGAAG AAAAGAGGAA 
AAGGCTGAAA AAGGAAGGTG TCATTTGGAG CAAGATAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308756.1
CDS	313..5454
Translation	MNGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSSDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSDNSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVNGASGLMTLNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRISTQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVIHGRNMGGVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSPRGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILEENFRYNNYKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLENSLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEAYSRVRKRNRKLRGRRKEEKAEKGRCHLEQDN

Target ORF information:

RefSeq Version	XM_024452988.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452988.1

ORF Insert Sequence:

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAACTGA GGGGAAGAAG AAAAGAGGAA 
AAGGCTGAAA AAGGAAGGTG TCATTTGGAG CAAGATAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_017004483.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859972.1 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

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CloneID	OHu69743
Clone ID Related Accession (Same CDS sequence)	XM_024452988.1 , XM_017004483.1
Accession Version	XM_017004483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAACTGA GGGGAAGAAG AAAAGAGGAA 
AAGGCTGAAA AAGGAAGGTG TCATTTGGAG CAAGATAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859972.1
CDS	680..5821
Translation	MNGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSSDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSDNSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVNGASGLMTLNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRISTQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVIHGRNMGGVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSPRGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILEENFRYNNYKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLENSLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEAYSRVRKRNRKLRGRRKEEKAEKGRCHLEQDN

Target ORF information:

RefSeq Version	XM_017004483.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004483.1

ORF Insert Sequence:

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAACTGA GGGGAAGAAG AAAAGAGGAA 
AAGGCTGAAA AAGGAAGGTG TCATTTGGAG CAAGATAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_017004484.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859973.1 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.

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CloneID	OHu69744
Clone ID Related Accession (Same CDS sequence)	XM_017004484.1 , XM_024452989.1
Accession Version	XM_017004484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAACTG 
AGGGGAAGAA GAAAAGAGGA AAAGGCTGAA AAAGGAAGGT GTCATTTGGA GCAAGATAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859973.1
CDS	680..5782
Translation	MNGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSSDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSDNSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVNGASGLMTLNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRISTQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVIHGRNMGGVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSPRGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILEENFRYNNYKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLENSLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRVRKRNRKLRGRRKEEKAEKGRCHLEQDN

Target ORF information:

RefSeq Version	XM_017004484.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004484.1

ORF Insert Sequence:

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAACTG 
AGGGGAAGAA GAAAAGAGGA AAAGGCTGAA AAAGGAAGGT GTCATTTGGA GCAAGATAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_024452989.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308757.1 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu69744
Clone ID Related Accession (Same CDS sequence)	XM_017004484.1 , XM_024452989.1
Accession Version	XM_024452989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAACTG 
AGGGGAAGAA GAAAAGAGGA AAAGGCTGAA AAAGGAAGGT GTCATTTGGA GCAAGATAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308757.1
CDS	313..5415
Translation	MNGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSSDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSDNSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVNGASGLMTLNPQLLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRISTQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVIHGRNMGGVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSPRGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILEENFRYNNYKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLENSLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRVRKRNRKLRGRRKEEKAEKGRCHLEQDN

Target ORF information:

RefSeq Version	XM_024452989.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452989.1

ORF Insert Sequence:

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAACTG 
AGGGGAAGAA GAAAAGAGGA AAAGGCTGAA AAAGGAAGGT GTCATTTGGA GCAAGATAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_024452990.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308758.1 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu69745
Clone ID Related Accession (Same CDS sequence)	XM_024452990.1 , XM_017004485.1
Accession Version	XM_024452990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC 
CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG 
CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA 
ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA 
ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA 
CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA 
CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA 
GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG 
CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG 
AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC 
AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT 
TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT 
GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG 
CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC 
CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG 
CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA 
GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA 
GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA 
TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT 
TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT 
AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT 
GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA 
AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT 
GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG 
AAGGTCTGGG TAATGTGGTT TGGTCTTCAT ACCTTCACTC AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT 
GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA 
ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC 
AAGAGGAGGA AGATCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308758.1
CDS	313..4836
Translation	MNGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRISTQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVIHGRNMGGVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSPRGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILEENFRYNNYKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLENSLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEAYSRVRKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	XM_024452990.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452990.1

ORF Insert Sequence:

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC 
CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG 
CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA 
ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA 
ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA 
CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA 
CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA 
GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG 
CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG 
AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC 
AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT 
TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT 
GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG 
CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC 
CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG 
CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA 
GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA 
GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA 
TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT 
TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT 
AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT 
GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA 
AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT 
GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG 
AAGGTCTGGG TAATGTGGTT TGGTCTTCAT ACCTTCACTC AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT 
GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA 
ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC 
AAGAGGAGGA AGATCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD5 ( XM_017004485.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859974.1 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA.

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CloneID	OHu69745
Clone ID Related Accession (Same CDS sequence)	XM_024452990.1 , XM_017004485.1
Accession Version	XM_017004485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC 
CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG 
CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA 
ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA 
ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA 
CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA 
CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA 
GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG 
CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG 
AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC 
AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT 
TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT 
GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG 
CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC 
CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG 
CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA 
GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA 
GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA 
TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT 
TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT 
AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT 
GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA 
AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT 
GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG 
AAGGTCTGGG TAATGTGGTT TGGTCTTCAT ACCTTCACTC AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT 
GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA 
ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC 
AAGAGGAGGA AGATCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859974.1
CDS	680..5203
Translation	MNGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQQQQQLLQGYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRISTQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVIHGRNMGGVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSPRGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILEENFRYNNYKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLENSLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVWVMWFGLHTFTQVEPEKLKTLTEGLEAYSRVRKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

Target ORF information:

RefSeq Version	XM_017004485.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004485.1

ORF Insert Sequence:

ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC 
CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG 
CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA 
ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA 
ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA 
CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA 
CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA 
GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG 
CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG 
AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC 
AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT 
TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT 
GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG 
CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC 
CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG 
CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA 
GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA 
GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA 
TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT 
TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT 
AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT 
GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA 
AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT 
GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG 
AAGGTCTGGG TAATGTGGTT TGGTCTTCAT ACCTTCACTC AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT 
GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA 
ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC 
AAGAGGAGGA AGATCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MBD5
Entrez Gene ID	55777
Full Name	methyl-CpG binding domain protein 5
Synonyms	MRD1
General protein information	Preferred Names methyl-CpG binding domain protein 5 Names methyl-CpG-binding domain protein 5 methyl-CpG-binding protein MBD5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q23.1
Summary	This gene encodes a member of the methyl-CpG-binding domain (MBD) family. The MBD consists of about 70 residues and is the minimal region required for a methyl-CpG-binding protein binding specifically to methylated DNA. In addition to the MBD domain, this protein contains a PWWP domain (Pro-Trp-Trp-Pro motif), which consists of 100-150 amino acids and is found in numerous proteins that are involved in cell division, growth and differentiation. Mutations in this gene cause an autosomal dominant type of cognitive disability. The encoded protein interacts with the polycomb repressive complex PR-DUB which catalyzes the deubiquitination of a lysine residue of histone 2A. Haploinsufficiency of this gene is associated with a syndrome involving microcephaly, intellectual disabilities, severe speech impairment, and seizures. Alternatively spliced transcript variants have been found, but their full-length nature is not determined. [provided by RefSeq, Jul 2017].
Disorder MIM:	611472
Disorder Html:	Mental retardation, autosomal dominant 1, 156200 (3)

Homo sapiens (human)	MBD5	NP_060798.2
Pan troglodytes (chimpanzee)	MBD5	XP_003309303.1
Mus musculus (house mouse)	Mbd5	NP_084200.1
Xenopus tropicalis (tropical clawed frog)	mbd5	XP_002943521.2
	MBD5	XP_002798916.1
Bos taurus (cattle)	MBD5	NP_001178197.1
Gallus gallus (chicken)	MBD5	XP_004943074.1
Canis lupus familiaris (dog)	MBD5	XP_541030.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MBD5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

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