MBD5 cDNA ORF clone, Gallus gallus(chicken)

The following MBD5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MBD5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa38601 XM_015289883.2
Latest version!
Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa38602 XM_015289884.2
Latest version!
Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa38603 XM_015289885.2
Latest version!
Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa11434 XM_025152451.1
Latest version!
Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa11434 XM_015289881.2
Latest version!
Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa11434 XM_025152452.1
Latest version!
Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa11434 XM_015289882.2
Latest version!
Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa11434 XM_015289878.2
Latest version!
Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa11434 XM_025152449.1
Latest version!
Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa11434 XM_015289876.2
Latest version!
Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa11434 XM_015289877.2
Latest version!
Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa11435 XM_004943022.3
Latest version!
Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa11437 XM_422153.6
Latest version!
Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OGa38603 XM_015289885.1 Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa11434 XM_015289881.1 Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa11434 XM_015289878.1 Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa11434 XM_015289876.1 Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa38601 XM_015289883.1 Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa11434 XM_015289882.1 Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa11434 XM_015289877.1 Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa11435 XM_004943022.2 Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa38602 XM_015289884.1 Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa11437 XM_422153.5 Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa38601
    Clone ID Related Accession (Same CDS sequence) XM_015289883.1 , XM_015289883.2
    Accession Version XM_015289883.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAG
    TCCACCAAAT CCCACAGGGA GACAGACAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145369.1
    CDS1516..6648
    Translation

    Target ORF information:

    RefSeq Version XM_015289883.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289883.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAG
    TCCACCAAAT CCCACAGGGA GACAGACAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38601
    Clone ID Related Accession (Same CDS sequence) XM_015289883.1 , XM_015289883.2
    Accession Version XM_015289883.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289883.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAG
    TCCACCAAAT CCCACAGGGA GACAGACAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145369.1
    CDS1366..6498
    Translation

    Target ORF information:

    RefSeq Version XM_015289883.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289883.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAG
    TCCACCAAAT CCCACAGGGA GACAGACAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38602
    Clone ID Related Accession (Same CDS sequence) XM_015289884.1 , XM_015289884.2
    Accession Version XM_015289884.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5094bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTGG AGCCAGAGAA GTTGAAGACA CTAACAGAAG GTCTAGAAGC TTACAACCGA
    GCCAGGAAAA GGAACAGAAA GTCCACCAAA TCCCACAGGG AGACAGACAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145370.1
    CDS1516..6609
    Translation

    Target ORF information:

    RefSeq Version XM_015289884.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTGG AGCCAGAGAA GTTGAAGACA CTAACAGAAG GTCTAGAAGC TTACAACCGA
    GCCAGGAAAA GGAACAGAAA GTCCACCAAA TCCCACAGGG AGACAGACAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38602
    Clone ID Related Accession (Same CDS sequence) XM_015289884.1 , XM_015289884.2
    Accession Version XM_015289884.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5094bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289884.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTGG AGCCAGAGAA GTTGAAGACA CTAACAGAAG GTCTAGAAGC TTACAACCGA
    GCCAGGAAAA GGAACAGAAA GTCCACCAAA TCCCACAGGG AGACAGACAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145370.1
    CDS1366..6459
    Translation

    Target ORF information:

    RefSeq Version XM_015289884.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289884.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTGG AGCCAGAGAA GTTGAAGACA CTAACAGAAG GTCTAGAAGC TTACAACCGA
    GCCAGGAAAA GGAACAGAAA GTCCACCAAA TCCCACAGGG AGACAGACAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38603
    Clone ID Related Accession (Same CDS sequence) XM_015289885.1 , XM_015289885.2
    Accession Version XM_015289885.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGTGACAT GTCATCTATA
    AACACTACTT TGAATAACCA TCAACTGAGT CATCTCCAGT CACTATTAAA CAACAATCAG
    ATGTTTCCTT CAAATCAGCA GCAACAGCAC CTTCTCCAGG GGTATCAGAA CATGCAGGGC
    TTTCAAGGCC AGCCCCCAAT TCCTGGCCCA GCTAACAACC CAAACCCCAT GGCATGTCTG
    TTCCAAAATT TCCAGGTGAG AATGCAGGAA GATGCTGCTG CCCTGAACAA AAGAATGATC
    ACTCAGATGG GAATGGCACC AGTTCCTGAG AGCTCCAACG CTATGCTTCC TCCTTTCCAA
    GAAACATCTT GTGATTTGCA GCAAAGAACT GAACCATCTC TTGGTCAACA GGCAAAGGAT
    AACCTCAATG TCGCTGCTCA GGGTGATACA TCAGTGGATG CGATCTACAA AGCAGTCGTA
    GATGCTGCAA GCAAAGGAAT GCAAGTAGTG ATCACCACTG CCGTTAGCAG TACCACACAG
    ATGAGTCCCA TTCCAGCTTT GAGTGCCATG AGTGCCTTCA CAGCCTCAAT TGGTGACCCG
    CTAAATCTCT CTAGTGCTGT CAGTGCAGTA ATCCATGGAA GAAACATTGC TGTTTCTGAT
    CATGAAGGTA GGATAAGAAA CACTAGAGGA ACGCGAGTAC TGAAGAATTC GGAGCATGGT
    AAAAATTCAA GTGAAGGGGA TGGGTATGAA TATTACAAAT CAACAAGTTG TAACACGCCC
    AAAAAACAGT GGGAAGGGGA GCAAAGTCCT GTCGGTGAGA TAAATAGGTG GAAATGTGAG
    GAGTTTCTAG ACCACTCCAC CCATATCCAT AGTAGTCCTT GTCACGAAAG GCCCAATAAC
    ATCTCCACAC TGCCATTATT GCAAGGTGAG CAGCATCAGA TACTGTTATC ACAGAGAAAC
    TGTCAAAGTG ATAAAATGTT GGAGGAGAAT TTCAGGTATA ACAATTACAA AAGAACTATG
    ATGAGTTTTA AGGAAAGACT GGAGAACACT GTGGAACGCT GTGCACACAT AAATGGAAAT
    AGGCCTCAGC AGAACAGAGG ATACGGGGAG TTGCTGAACA CTTCTAAACA AGACCTGATT
    CTGGAAGAGC AATCTCCAAG TTCCTCAAAT AGTTTGGAAA GTTCGTTAGT TAAAGACTAC
    ATCCATTACA ATGGAGATTT TAATGCCAAA AGCATTAATG GGTGTGTGCC TAGCCCTTCA
    GATGCTAAAA GCATCAGTAG TGAAGATGAC CTAAGGAATC CAGATTCCCC TTCTTCAAAT
    GAGCTGATAC ATTACAGGCC GAGGACGTTC AATGTTGGCG ACTTGGTCTG GGGCCAAATC
    AAAGGACTGA CTTCATGGCC TGGAAAACTA GTAAGAGAAG AAGACGTTCA CAATTCATGT
    CAACAGAACG CTGAGGAGGG GAAGGTCTGG GTAATGTGGT TTGGTGTTCA TACCTTCACT
    CAGGTGGAGC CAGAGAAGTT GAAGACACTA ACAGAAGGTC TAGAAGCTTA CAACCGAGCC
    AGGAAAAGGA ACAGAAAAAG TGGAAAGCTA AATAACCATT TAGAAGCTGC TATACACGAG
    GCCATGAGTG AGCTAGACAA AATGTCTGGG AATGTCCACC AAATCCCACA GGGAGACAGA
    CAAGTGAAGC CTCCAAAACC CAAGAGGAGG AAGATCTCTA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145371.1
    CDS1516..6060
    Translation

    Target ORF information:

    RefSeq Version XM_015289885.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289885.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGTGACAT GTCATCTATA
    AACACTACTT TGAATAACCA TCAACTGAGT CATCTCCAGT CACTATTAAA CAACAATCAG
    ATGTTTCCTT CAAATCAGCA GCAACAGCAC CTTCTCCAGG GGTATCAGAA CATGCAGGGC
    TTTCAAGGCC AGCCCCCAAT TCCTGGCCCA GCTAACAACC CAAACCCCAT GGCATGTCTG
    TTCCAAAATT TCCAGGTGAG AATGCAGGAA GATGCTGCTG CCCTGAACAA AAGAATGATC
    ACTCAGATGG GAATGGCACC AGTTCCTGAG AGCTCCAACG CTATGCTTCC TCCTTTCCAA
    GAAACATCTT GTGATTTGCA GCAAAGAACT GAACCATCTC TTGGTCAACA GGCAAAGGAT
    AACCTCAATG TCGCTGCTCA GGGTGATACA TCAGTGGATG CGATCTACAA AGCAGTCGTA
    GATGCTGCAA GCAAAGGAAT GCAAGTAGTG ATCACCACTG CCGTTAGCAG TACCACACAG
    ATGAGTCCCA TTCCAGCTTT GAGTGCCATG AGTGCCTTCA CAGCCTCAAT TGGTGACCCG
    CTAAATCTCT CTAGTGCTGT CAGTGCAGTA ATCCATGGAA GAAACATTGC TGTTTCTGAT
    CATGAAGGTA GGATAAGAAA CACTAGAGGA ACGCGAGTAC TGAAGAATTC GGAGCATGGT
    AAAAATTCAA GTGAAGGGGA TGGGTATGAA TATTACAAAT CAACAAGTTG TAACACGCCC
    AAAAAACAGT GGGAAGGGGA GCAAAGTCCT GTCGGTGAGA TAAATAGGTG GAAATGTGAG
    GAGTTTCTAG ACCACTCCAC CCATATCCAT AGTAGTCCTT GTCACGAAAG GCCCAATAAC
    ATCTCCACAC TGCCATTATT GCAAGGTGAG CAGCATCAGA TACTGTTATC ACAGAGAAAC
    TGTCAAAGTG ATAAAATGTT GGAGGAGAAT TTCAGGTATA ACAATTACAA AAGAACTATG
    ATGAGTTTTA AGGAAAGACT GGAGAACACT GTGGAACGCT GTGCACACAT AAATGGAAAT
    AGGCCTCAGC AGAACAGAGG ATACGGGGAG TTGCTGAACA CTTCTAAACA AGACCTGATT
    CTGGAAGAGC AATCTCCAAG TTCCTCAAAT AGTTTGGAAA GTTCGTTAGT TAAAGACTAC
    ATCCATTACA ATGGAGATTT TAATGCCAAA AGCATTAATG GGTGTGTGCC TAGCCCTTCA
    GATGCTAAAA GCATCAGTAG TGAAGATGAC CTAAGGAATC CAGATTCCCC TTCTTCAAAT
    GAGCTGATAC ATTACAGGCC GAGGACGTTC AATGTTGGCG ACTTGGTCTG GGGCCAAATC
    AAAGGACTGA CTTCATGGCC TGGAAAACTA GTAAGAGAAG AAGACGTTCA CAATTCATGT
    CAACAGAACG CTGAGGAGGG GAAGGTCTGG GTAATGTGGT TTGGTGTTCA TACCTTCACT
    CAGGTGGAGC CAGAGAAGTT GAAGACACTA ACAGAAGGTC TAGAAGCTTA CAACCGAGCC
    AGGAAAAGGA ACAGAAAAAG TGGAAAGCTA AATAACCATT TAGAAGCTGC TATACACGAG
    GCCATGAGTG AGCTAGACAA AATGTCTGGG AATGTCCACC AAATCCCACA GGGAGACAGA
    CAAGTGAAGC CTCCAAAACC CAAGAGGAGG AAGATCTCTA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38603
    Clone ID Related Accession (Same CDS sequence) XM_015289885.1 , XM_015289885.2
    Accession Version XM_015289885.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289885.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGTGACAT GTCATCTATA
    AACACTACTT TGAATAACCA TCAACTGAGT CATCTCCAGT CACTATTAAA CAACAATCAG
    ATGTTTCCTT CAAATCAGCA GCAACAGCAC CTTCTCCAGG GGTATCAGAA CATGCAGGGC
    TTTCAAGGCC AGCCCCCAAT TCCTGGCCCA GCTAACAACC CAAACCCCAT GGCATGTCTG
    TTCCAAAATT TCCAGGTGAG AATGCAGGAA GATGCTGCTG CCCTGAACAA AAGAATGATC
    ACTCAGATGG GAATGGCACC AGTTCCTGAG AGCTCCAACG CTATGCTTCC TCCTTTCCAA
    GAAACATCTT GTGATTTGCA GCAAAGAACT GAACCATCTC TTGGTCAACA GGCAAAGGAT
    AACCTCAATG TCGCTGCTCA GGGTGATACA TCAGTGGATG CGATCTACAA AGCAGTCGTA
    GATGCTGCAA GCAAAGGAAT GCAAGTAGTG ATCACCACTG CCGTTAGCAG TACCACACAG
    ATGAGTCCCA TTCCAGCTTT GAGTGCCATG AGTGCCTTCA CAGCCTCAAT TGGTGACCCG
    CTAAATCTCT CTAGTGCTGT CAGTGCAGTA ATCCATGGAA GAAACATTGC TGTTTCTGAT
    CATGAAGGTA GGATAAGAAA CACTAGAGGA ACGCGAGTAC TGAAGAATTC GGAGCATGGT
    AAAAATTCAA GTGAAGGGGA TGGGTATGAA TATTACAAAT CAACAAGTTG TAACACGCCC
    AAAAAACAGT GGGAAGGGGA GCAAAGTCCT GTCGGTGAGA TAAATAGGTG GAAATGTGAG
    GAGTTTCTAG ACCACTCCAC CCATATCCAT AGTAGTCCTT GTCACGAAAG GCCCAATAAC
    ATCTCCACAC TGCCATTATT GCAAGGTGAG CAGCATCAGA TACTGTTATC ACAGAGAAAC
    TGTCAAAGTG ATAAAATGTT GGAGGAGAAT TTCAGGTATA ACAATTACAA AAGAACTATG
    ATGAGTTTTA AGGAAAGACT GGAGAACACT GTGGAACGCT GTGCACACAT AAATGGAAAT
    AGGCCTCAGC AGAACAGAGG ATACGGGGAG TTGCTGAACA CTTCTAAACA AGACCTGATT
    CTGGAAGAGC AATCTCCAAG TTCCTCAAAT AGTTTGGAAA GTTCGTTAGT TAAAGACTAC
    ATCCATTACA ATGGAGATTT TAATGCCAAA AGCATTAATG GGTGTGTGCC TAGCCCTTCA
    GATGCTAAAA GCATCAGTAG TGAAGATGAC CTAAGGAATC CAGATTCCCC TTCTTCAAAT
    GAGCTGATAC ATTACAGGCC GAGGACGTTC AATGTTGGCG ACTTGGTCTG GGGCCAAATC
    AAAGGACTGA CTTCATGGCC TGGAAAACTA GTAAGAGAAG AAGACGTTCA CAATTCATGT
    CAACAGAACG CTGAGGAGGG GAAGGTCTGG GTAATGTGGT TTGGTGTTCA TACCTTCACT
    CAGGTGGAGC CAGAGAAGTT GAAGACACTA ACAGAAGGTC TAGAAGCTTA CAACCGAGCC
    AGGAAAAGGA ACAGAAAAAG TGGAAAGCTA AATAACCATT TAGAAGCTGC TATACACGAG
    GCCATGAGTG AGCTAGACAA AATGTCTGGG AATGTCCACC AAATCCCACA GGGAGACAGA
    CAAGTGAAGC CTCCAAAACC CAAGAGGAGG AAGATCTCTA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145371.1
    CDS1366..5910
    Translation

    Target ORF information:

    RefSeq Version XM_015289885.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289885.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGTGACAT GTCATCTATA
    AACACTACTT TGAATAACCA TCAACTGAGT CATCTCCAGT CACTATTAAA CAACAATCAG
    ATGTTTCCTT CAAATCAGCA GCAACAGCAC CTTCTCCAGG GGTATCAGAA CATGCAGGGC
    TTTCAAGGCC AGCCCCCAAT TCCTGGCCCA GCTAACAACC CAAACCCCAT GGCATGTCTG
    TTCCAAAATT TCCAGGTGAG AATGCAGGAA GATGCTGCTG CCCTGAACAA AAGAATGATC
    ACTCAGATGG GAATGGCACC AGTTCCTGAG AGCTCCAACG CTATGCTTCC TCCTTTCCAA
    GAAACATCTT GTGATTTGCA GCAAAGAACT GAACCATCTC TTGGTCAACA GGCAAAGGAT
    AACCTCAATG TCGCTGCTCA GGGTGATACA TCAGTGGATG CGATCTACAA AGCAGTCGTA
    GATGCTGCAA GCAAAGGAAT GCAAGTAGTG ATCACCACTG CCGTTAGCAG TACCACACAG
    ATGAGTCCCA TTCCAGCTTT GAGTGCCATG AGTGCCTTCA CAGCCTCAAT TGGTGACCCG
    CTAAATCTCT CTAGTGCTGT CAGTGCAGTA ATCCATGGAA GAAACATTGC TGTTTCTGAT
    CATGAAGGTA GGATAAGAAA CACTAGAGGA ACGCGAGTAC TGAAGAATTC GGAGCATGGT
    AAAAATTCAA GTGAAGGGGA TGGGTATGAA TATTACAAAT CAACAAGTTG TAACACGCCC
    AAAAAACAGT GGGAAGGGGA GCAAAGTCCT GTCGGTGAGA TAAATAGGTG GAAATGTGAG
    GAGTTTCTAG ACCACTCCAC CCATATCCAT AGTAGTCCTT GTCACGAAAG GCCCAATAAC
    ATCTCCACAC TGCCATTATT GCAAGGTGAG CAGCATCAGA TACTGTTATC ACAGAGAAAC
    TGTCAAAGTG ATAAAATGTT GGAGGAGAAT TTCAGGTATA ACAATTACAA AAGAACTATG
    ATGAGTTTTA AGGAAAGACT GGAGAACACT GTGGAACGCT GTGCACACAT AAATGGAAAT
    AGGCCTCAGC AGAACAGAGG ATACGGGGAG TTGCTGAACA CTTCTAAACA AGACCTGATT
    CTGGAAGAGC AATCTCCAAG TTCCTCAAAT AGTTTGGAAA GTTCGTTAGT TAAAGACTAC
    ATCCATTACA ATGGAGATTT TAATGCCAAA AGCATTAATG GGTGTGTGCC TAGCCCTTCA
    GATGCTAAAA GCATCAGTAG TGAAGATGAC CTAAGGAATC CAGATTCCCC TTCTTCAAAT
    GAGCTGATAC ATTACAGGCC GAGGACGTTC AATGTTGGCG ACTTGGTCTG GGGCCAAATC
    AAAGGACTGA CTTCATGGCC TGGAAAACTA GTAAGAGAAG AAGACGTTCA CAATTCATGT
    CAACAGAACG CTGAGGAGGG GAAGGTCTGG GTAATGTGGT TTGGTGTTCA TACCTTCACT
    CAGGTGGAGC CAGAGAAGTT GAAGACACTA ACAGAAGGTC TAGAAGCTTA CAACCGAGCC
    AGGAAAAGGA ACAGAAAAAG TGGAAAGCTA AATAACCATT TAGAAGCTGC TATACACGAG
    GCCATGAGTG AGCTAGACAA AATGTCTGGG AATGTCCACC AAATCCCACA GGGAGACAGA
    CAAGTGAAGC CTCCAAAACC CAAGAGGAGG AAGATCTCTA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11434
    Clone ID Related Accession (Same CDS sequence) XM_015289878.1 , XM_015289881.2 , XM_015289877.1 , XM_015289881.1 , XM_015289878.2 , XM_025152451.1 , XM_015289876.1 , XM_015289876.2 , XM_015289882.2 , XM_015289882.1 , XM_025152449.1 , XM_015289877.2 , XM_025152452.1
    Accession Version XM_015289878.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145364.1
    CDS1091..6337
    Translation

    Target ORF information:

    RefSeq Version XM_015289878.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289878.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11434
    Clone ID Related Accession (Same CDS sequence) XM_015289878.1 , XM_015289881.2 , XM_015289877.1 , XM_015289881.1 , XM_015289878.2 , XM_025152451.1 , XM_015289876.1 , XM_015289876.2 , XM_015289882.2 , XM_015289882.1 , XM_025152449.1 , XM_015289877.2 , XM_025152452.1
    Accession Version XM_015289881.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145367.1
    CDS1236..6482
    Translation

    Target ORF information:

    RefSeq Version XM_015289881.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289881.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11434
    Clone ID Related Accession (Same CDS sequence) XM_015289878.1 , XM_015289881.2 , XM_015289877.1 , XM_015289881.1 , XM_015289878.2 , XM_025152451.1 , XM_015289876.1 , XM_015289876.2 , XM_015289882.2 , XM_015289882.1 , XM_025152449.1 , XM_015289877.2 , XM_025152452.1
    Accession Version XM_015289877.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145363.1
    CDS1221..6467
    Translation

    Target ORF information:

    RefSeq Version XM_015289877.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11434
    Clone ID Related Accession (Same CDS sequence) XM_015289878.1 , XM_015289881.2 , XM_015289877.1 , XM_015289881.1 , XM_015289878.2 , XM_025152451.1 , XM_015289876.1 , XM_015289876.2 , XM_015289882.2 , XM_015289882.1 , XM_025152449.1 , XM_015289877.2 , XM_025152452.1
    Accession Version XM_015289881.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145367.1
    CDS1386..6632
    Translation

    Target ORF information:

    RefSeq Version XM_015289881.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289881.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11434
    Clone ID Related Accession (Same CDS sequence) XM_015289878.1 , XM_015289881.2 , XM_015289877.1 , XM_015289881.1 , XM_015289878.2 , XM_025152451.1 , XM_015289876.1 , XM_015289876.2 , XM_015289882.2 , XM_015289882.1 , XM_025152449.1 , XM_015289877.2 , XM_025152452.1
    Accession Version XM_015289878.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289878.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145364.1
    CDS941..6187
    Translation

    Target ORF information:

    RefSeq Version XM_015289878.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289878.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11434
    Clone ID Related Accession (Same CDS sequence) XM_015289878.1 , XM_015289881.2 , XM_015289877.1 , XM_015289881.1 , XM_015289878.2 , XM_025152451.1 , XM_015289876.1 , XM_015289876.2 , XM_015289882.2 , XM_015289882.1 , XM_025152449.1 , XM_015289877.2 , XM_025152452.1
    Accession Version XM_025152451.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025008219.1
    CDS883..6129
    Translation

    Target ORF information:

    RefSeq Version XM_025152451.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025152451.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11434
    Clone ID Related Accession (Same CDS sequence) XM_015289878.1 , XM_015289881.2 , XM_015289877.1 , XM_015289881.1 , XM_015289878.2 , XM_025152451.1 , XM_015289876.1 , XM_015289876.2 , XM_015289882.2 , XM_015289882.1 , XM_025152449.1 , XM_015289877.2 , XM_025152452.1
    Accession Version XM_015289876.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145362.1
    CDS1458..6704
    Translation

    Target ORF information:

    RefSeq Version XM_015289876.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289876.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11434
    Clone ID Related Accession (Same CDS sequence) XM_015289878.1 , XM_015289881.2 , XM_015289877.1 , XM_015289881.1 , XM_015289878.2 , XM_025152451.1 , XM_015289876.1 , XM_015289876.2 , XM_015289882.2 , XM_015289882.1 , XM_025152449.1 , XM_015289877.2 , XM_025152452.1
    Accession Version XM_015289876.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289876.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145362.1
    CDS1308..6554
    Translation

    Target ORF information:

    RefSeq Version XM_015289876.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289876.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11434
    Clone ID Related Accession (Same CDS sequence) XM_015289878.1 , XM_015289881.2 , XM_015289877.1 , XM_015289881.1 , XM_015289878.2 , XM_025152451.1 , XM_015289876.1 , XM_015289876.2 , XM_015289882.2 , XM_015289882.1 , XM_025152449.1 , XM_015289877.2 , XM_025152452.1
    Accession Version XM_015289882.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145368.1
    CDS926..6172
    Translation

    Target ORF information:

    RefSeq Version XM_015289882.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289882.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11434
    Clone ID Related Accession (Same CDS sequence) XM_015289878.1 , XM_015289881.2 , XM_015289877.1 , XM_015289881.1 , XM_015289878.2 , XM_025152451.1 , XM_015289876.1 , XM_015289876.2 , XM_015289882.2 , XM_015289882.1 , XM_025152449.1 , XM_015289877.2 , XM_025152452.1
    Accession Version XM_015289882.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145368.1
    CDS1076..6322
    Translation

    Target ORF information:

    RefSeq Version XM_015289882.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289882.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11434
    Clone ID Related Accession (Same CDS sequence) XM_015289878.1 , XM_015289881.2 , XM_015289877.1 , XM_015289881.1 , XM_015289878.2 , XM_025152451.1 , XM_015289876.1 , XM_015289876.2 , XM_015289882.2 , XM_015289882.1 , XM_025152449.1 , XM_015289877.2 , XM_025152452.1
    Accession Version XM_025152449.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025008217.1
    CDS1366..6612
    Translation

    Target ORF information:

    RefSeq Version XM_025152449.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025152449.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11434
    Clone ID Related Accession (Same CDS sequence) XM_015289878.1 , XM_015289881.2 , XM_015289877.1 , XM_015289881.1 , XM_015289878.2 , XM_025152451.1 , XM_015289876.1 , XM_015289876.2 , XM_015289882.2 , XM_015289882.1 , XM_025152449.1 , XM_015289877.2 , XM_025152452.1
    Accession Version XM_015289877.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289877.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145363.1
    CDS1071..6317
    Translation

    Target ORF information:

    RefSeq Version XM_015289877.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289877.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11434
    Clone ID Related Accession (Same CDS sequence) XM_015289878.1 , XM_015289881.2 , XM_015289877.1 , XM_015289881.1 , XM_015289878.2 , XM_025152451.1 , XM_015289876.1 , XM_015289876.2 , XM_015289882.2 , XM_015289882.1 , XM_025152449.1 , XM_015289877.2 , XM_025152452.1
    Accession Version XM_025152452.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025008220.1
    CDS868..6114
    Translation

    Target ORF information:

    RefSeq Version XM_025152452.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025152452.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTCT GGGTAATGTG GTTTGGTGTT CATACCTTCA CTCAGGTGGA GCCAGAGAAG
    TTGAAGACAC TAACAGAAGG TCTAGAAGCT TACAACCGAG CCAGGAAAAG GAACAGAAAA
    AGTGGAAAGC TAAATAACCA TTTAGAAGCT GCTATACACG AGGCCATGAG TGAGCTAGAC
    AAAATGTCTG GGAATGTCCA CCAAATCCCA CAGGGAGACA GACAAGTGAA GCCTCCAAAA
    CCCAAGAGGA GGAAGATCTC TAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11435
    Clone ID Related Accession (Same CDS sequence) XM_004943022.2 , XM_004943022.3
    Accession Version XM_004943022.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTGG AGCCAGAGAA GTTGAAGACA CTAACAGAAG GTCTAGAAGC TTACAACCGA
    GCCAGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATACAC
    GAGGCCATGA GTGAGCTAGA CAAAATGTCT GGGAATGTCC ACCAAATCCC ACAGGGAGAC
    AGACAAGTGA AGCCTCCAAA ACCCAAGAGG AGGAAGATCT CTAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943079.1
    CDS1516..6723
    Translation

    Target ORF information:

    RefSeq Version XM_004943022.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943022.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTGG AGCCAGAGAA GTTGAAGACA CTAACAGAAG GTCTAGAAGC TTACAACCGA
    GCCAGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATACAC
    GAGGCCATGA GTGAGCTAGA CAAAATGTCT GGGAATGTCC ACCAAATCCC ACAGGGAGAC
    AGACAAGTGA AGCCTCCAAA ACCCAAGAGG AGGAAGATCT CTAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11435
    Clone ID Related Accession (Same CDS sequence) XM_004943022.2 , XM_004943022.3
    Accession Version XM_004943022.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943022.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTGG AGCCAGAGAA GTTGAAGACA CTAACAGAAG GTCTAGAAGC TTACAACCGA
    GCCAGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATACAC
    GAGGCCATGA GTGAGCTAGA CAAAATGTCT GGGAATGTCC ACCAAATCCC ACAGGGAGAC
    AGACAAGTGA AGCCTCCAAA ACCCAAGAGG AGGAAGATCT CTAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943079.1
    CDS1366..6573
    Translation

    Target ORF information:

    RefSeq Version XM_004943022.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943022.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGCAAGTC TGAGGTCAAC
    CTCAACCCTT TAGGTTTTCT CAACCCGAAT GTAAATGCTG CTTTAGCTTT TCTCTCCAGT
    GACGTGGATG GGCAGGTATT ACAGCCTGTT CATTTTCAGC TTCTAGCAAC CCTCCTCCAG
    AACCAAGCCC AAGCAGCTGC CATGCTTCCC CTACCATCTT TCAATCTGAC CATCTCAGAT
    TTGTTGCAAC AGCAAAATAA CCCTTTGCCC TCAGTAACAC AGATGACAGC CCCACCTGAC
    CATTTGCCAA GCAATCAGTC AGAAAGCAAC AGGGTTGAGA CCCTTTTAAG CAACCCCCTG
    GGGAACCCTT TACCAAGCTT TTCAGGCACT GACACTACTT CTAACCCCCT GCTCCTCCCA
    GCTGTCTCTG GGGCCTCAGC ATTAATGGCC TTGAATCCCC AGCTGGTGGG AGGTGTCCTG
    AACTCTGCGT CGGGCAACAC CGCTAATCAT CCAGAGGTTT CCATAGCCAC CTCCTCCCAA
    GCAACCACTA CCACAACCAC TACATCATCA GCAGTGGCAG CACTGTCTGT CTCTACCCTG
    GGTGGTGGGA CAGCAGTGGT GTCAATGGCA GAAACATTGC TGAACATATC TAATAATGCT
    GGGAATACAT CTGGTCCAGC TAAACTCAAC AGTAACTCTG TGGTGCCACA GCTACTTAAC
    CCTCTACTGG GGACAGGTCT GCTTGGTGAC ATGTCATCTA TAAACACTAC TTTGAATAAC
    CATCAACTGA GTCATCTCCA GTCACTATTA AACAACAATC AGATGTTTCC TTCAAATCAG
    CAGCAACAGC ACCTTCTCCA GGGGTATCAG AACATGCAGG GCTTTCAAGG CCAGCCCCCA
    ATTCCTGGCC CAGCTAACAA CCCAAACCCC ATGGCATGTC TGTTCCAAAA TTTCCAGGTG
    AGAATGCAGG AAGATGCTGC TGCCCTGAAC AAAAGAATGA TCACTCAGAT GGGAATGGCA
    CCAGTTCCTG AGAGCTCCAA CGCTATGCTT CCTCCTTTCC AAGAAACATC TTGTGATTTG
    CAGCAAAGAA CTGAACCATC TCTTGGTCAA CAGGCAAAGG ATAACCTCAA TGTCGCTGCT
    CAGGGTGATA CATCAGTGGA TGCGATCTAC AAAGCAGTCG TAGATGCTGC AAGCAAAGGA
    ATGCAAGTAG TGATCACCAC TGCCGTTAGC AGTACCACAC AGATGAGTCC CATTCCAGCT
    TTGAGTGCCA TGAGTGCCTT CACAGCCTCA ATTGGTGACC CGCTAAATCT CTCTAGTGCT
    GTCAGTGCAG TAATCCATGG AAGAAACATT GCTGTTTCTG ATCATGAAGG TAGGATAAGA
    AACACTAGAG GAACGCGAGT ACTGAAGAAT TCGGAGCATG GTAAAAATTC AAGTGAAGGG
    GATGGGTATG AATATTACAA ATCAACAAGT TGTAACACGC CCAAAAAACA GTGGGAAGGG
    GAGCAAAGTC CTGTCGGTGA GATAAATAGG TGGAAATGTG AGGAGTTTCT AGACCACTCC
    ACCCATATCC ATAGTAGTCC TTGTCACGAA AGGCCCAATA ACATCTCCAC ACTGCCATTA
    TTGCAAGGTG AGCAGCATCA GATACTGTTA TCACAGAGAA ACTGTCAAAG TGATAAAATG
    TTGGAGGAGA ATTTCAGGTA TAACAATTAC AAAAGAACTA TGATGAGTTT TAAGGAAAGA
    CTGGAGAACA CTGTGGAACG CTGTGCACAC ATAAATGGAA ATAGGCCTCA GCAGAACAGA
    GGATACGGGG AGTTGCTGAA CACTTCTAAA CAAGACCTGA TTCTGGAAGA GCAATCTCCA
    AGTTCCTCAA ATAGTTTGGA AAGTTCGTTA GTTAAAGACT ACATCCATTA CAATGGAGAT
    TTTAATGCCA AAAGCATTAA TGGGTGTGTG CCTAGCCCTT CAGATGCTAA AAGCATCAGT
    AGTGAAGATG ACCTAAGGAA TCCAGATTCC CCTTCTTCAA ATGAGCTGAT ACATTACAGG
    CCGAGGACGT TCAATGTTGG CGACTTGGTC TGGGGCCAAA TCAAAGGACT GACTTCATGG
    CCTGGAAAAC TAGTAAGAGA AGAAGACGTT CACAATTCAT GTCAACAGAA CGCTGAGGAG
    GGGAAGGTGG AGCCAGAGAA GTTGAAGACA CTAACAGAAG GTCTAGAAGC TTACAACCGA
    GCCAGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATACAC
    GAGGCCATGA GTGAGCTAGA CAAAATGTCT GGGAATGTCC ACCAAATCCC ACAGGGAGAC
    AGACAAGTGA AGCCTCCAAA ACCCAAGAGG AGGAAGATCT CTAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11437
    Clone ID Related Accession (Same CDS sequence) XM_422153.5 , XM_422153.6
    Accession Version XM_422153.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_422153.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGTGACAT GTCATCTATA
    AACACTACTT TGAATAACCA TCAACTGAGT CATCTCCAGT CACTATTAAA CAACAATCAG
    ATGTTTCCTT CAAATCAGCA GCAACAGCAC CTTCTCCAGG GGTATCAGAA CATGCAGGGC
    TTTCAAGGCC AGCCCCCAAT TCCTGGCCCA GCTAACAACC CAAACCCCAT GGCATGTCTG
    TTCCAAAATT TCCAGGTGAG AATGCAGGAA GATGCTGCTG CCCTGAACAA AAGAATGATC
    ACTCAGATGG GAATGGCACC AGTTCCTGAG AGCTCCAACG CTATGCTTCC TCCTTTCCAA
    GAAACATCTT GTGATTTGCA GCAAAGAACT GAACCATCTC TTGGTCAACA GGCAAAGGAT
    AACCTCAATG TCGCTGCTCA GGGTGATACA TCAGTGGATG CGATCTACAA AGCAGTCGTA
    GATGCTGCAA GCAAAGGAAT GCAAGTAGTG ATCACCACTG CCGTTAGCAG TACCACACAG
    ATGAGTCCCA TTCCAGCTTT GAGTGCCATG AGTGCCTTCA CAGCCTCAAT TGGTGACCCG
    CTAAATCTCT CTAGTGCTGT CAGTGCAGTA ATCCATGGAA GAAACATTGC TGTTTCTGAT
    CATGAAGGTA GGATAAGAAA CACTAGAGGA ACGCGAGTAC TGAAGAATTC GGAGCATGGT
    AAAAATTCAA GTGAAGGGGA TGGGTATGAA TATTACAAAT CAACAAGTTG TAACACGCCC
    AAAAAACAGT GGGAAGGGGA GCAAAGTCCT GTCGGTGAGA TAAATAGGTG GAAATGTGAG
    GAGTTTCTAG ACCACTCCAC CCATATCCAT AGTAGTCCTT GTCACGAAAG GCCCAATAAC
    ATCTCCACAC TGCCATTATT GCAAGGTGAG CAGCATCAGA TACTGTTATC ACAGAGAAAC
    TGTCAAAGTG ATAAAATGTT GGAGGAGAAT TTCAGGTATA ACAATTACAA AAGAACTATG
    ATGAGTTTTA AGGAAAGACT GGAGAACACT GTGGAACGCT GTGCACACAT AAATGGAAAT
    AGGCCTCAGC AGAACAGAGG ATACGGGGAG TTGCTGAACA CTTCTAAACA AGACCTGATT
    CTGGAAGAGC AATCTCCAAG TTCCTCAAAT AGTTTGGAAA GTTCGTTAGT TAAAGACTAC
    ATCCATTACA ATGGAGATTT TAATGCCAAA AGCATTAATG GGTGTGTGCC TAGCCCTTCA
    GATGCTAAAA GCATCAGTAG TGAAGATGAC CTAAGGAATC CAGATTCCCC TTCTTCAAAT
    GAGCTGATAC ATTACAGGCC GAGGACGTTC AATGTTGGCG ACTTGGTCTG GGGCCAAATC
    AAAGGACTGA CTTCATGGCC TGGAAAACTA GTAAGAGAAG AAGACGTTCA CAATTCATGT
    CAACAGAACG CTGAGGAGGG GAAGGTGGAG CCAGAGAAGT TGAAGACACT AACAGAAGGT
    CTAGAAGCTT ACAACCGAGC CAGGAAAAGG AACAGAAAAA GTGGAAAGCT AAATAACCAT
    TTAGAAGCTG CTATACACGA GGCCATGAGT GAGCTAGACA AAATGTCTGG GAATGTCCAC
    CAAATCCCAC AGGGAGACAG ACAAGTGAAG CCTCCAAAAC CCAAGAGGAG GAAGATCTCT
    AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_422153.2
    CDS1516..6021
    Translation

    Target ORF information:

    RefSeq Version XM_422153.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_422153.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGTGACAT GTCATCTATA
    AACACTACTT TGAATAACCA TCAACTGAGT CATCTCCAGT CACTATTAAA CAACAATCAG
    ATGTTTCCTT CAAATCAGCA GCAACAGCAC CTTCTCCAGG GGTATCAGAA CATGCAGGGC
    TTTCAAGGCC AGCCCCCAAT TCCTGGCCCA GCTAACAACC CAAACCCCAT GGCATGTCTG
    TTCCAAAATT TCCAGGTGAG AATGCAGGAA GATGCTGCTG CCCTGAACAA AAGAATGATC
    ACTCAGATGG GAATGGCACC AGTTCCTGAG AGCTCCAACG CTATGCTTCC TCCTTTCCAA
    GAAACATCTT GTGATTTGCA GCAAAGAACT GAACCATCTC TTGGTCAACA GGCAAAGGAT
    AACCTCAATG TCGCTGCTCA GGGTGATACA TCAGTGGATG CGATCTACAA AGCAGTCGTA
    GATGCTGCAA GCAAAGGAAT GCAAGTAGTG ATCACCACTG CCGTTAGCAG TACCACACAG
    ATGAGTCCCA TTCCAGCTTT GAGTGCCATG AGTGCCTTCA CAGCCTCAAT TGGTGACCCG
    CTAAATCTCT CTAGTGCTGT CAGTGCAGTA ATCCATGGAA GAAACATTGC TGTTTCTGAT
    CATGAAGGTA GGATAAGAAA CACTAGAGGA ACGCGAGTAC TGAAGAATTC GGAGCATGGT
    AAAAATTCAA GTGAAGGGGA TGGGTATGAA TATTACAAAT CAACAAGTTG TAACACGCCC
    AAAAAACAGT GGGAAGGGGA GCAAAGTCCT GTCGGTGAGA TAAATAGGTG GAAATGTGAG
    GAGTTTCTAG ACCACTCCAC CCATATCCAT AGTAGTCCTT GTCACGAAAG GCCCAATAAC
    ATCTCCACAC TGCCATTATT GCAAGGTGAG CAGCATCAGA TACTGTTATC ACAGAGAAAC
    TGTCAAAGTG ATAAAATGTT GGAGGAGAAT TTCAGGTATA ACAATTACAA AAGAACTATG
    ATGAGTTTTA AGGAAAGACT GGAGAACACT GTGGAACGCT GTGCACACAT AAATGGAAAT
    AGGCCTCAGC AGAACAGAGG ATACGGGGAG TTGCTGAACA CTTCTAAACA AGACCTGATT
    CTGGAAGAGC AATCTCCAAG TTCCTCAAAT AGTTTGGAAA GTTCGTTAGT TAAAGACTAC
    ATCCATTACA ATGGAGATTT TAATGCCAAA AGCATTAATG GGTGTGTGCC TAGCCCTTCA
    GATGCTAAAA GCATCAGTAG TGAAGATGAC CTAAGGAATC CAGATTCCCC TTCTTCAAAT
    GAGCTGATAC ATTACAGGCC GAGGACGTTC AATGTTGGCG ACTTGGTCTG GGGCCAAATC
    AAAGGACTGA CTTCATGGCC TGGAAAACTA GTAAGAGAAG AAGACGTTCA CAATTCATGT
    CAACAGAACG CTGAGGAGGG GAAGGTGGAG CCAGAGAAGT TGAAGACACT AACAGAAGGT
    CTAGAAGCTT ACAACCGAGC CAGGAAAAGG AACAGAAAAA GTGGAAAGCT AAATAACCAT
    TTAGAAGCTG CTATACACGA GGCCATGAGT GAGCTAGACA AAATGTCTGG GAATGTCCAC
    CAAATCCCAC AGGGAGACAG ACAAGTGAAG CCTCCAAAAC CCAAGAGGAG GAAGATCTCT
    AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11437
    Clone ID Related Accession (Same CDS sequence) XM_422153.5 , XM_422153.6
    Accession Version XM_422153.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product methyl-CpG-binding domain protein 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_422153.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGTGACAT GTCATCTATA
    AACACTACTT TGAATAACCA TCAACTGAGT CATCTCCAGT CACTATTAAA CAACAATCAG
    ATGTTTCCTT CAAATCAGCA GCAACAGCAC CTTCTCCAGG GGTATCAGAA CATGCAGGGC
    TTTCAAGGCC AGCCCCCAAT TCCTGGCCCA GCTAACAACC CAAACCCCAT GGCATGTCTG
    TTCCAAAATT TCCAGGTGAG AATGCAGGAA GATGCTGCTG CCCTGAACAA AAGAATGATC
    ACTCAGATGG GAATGGCACC AGTTCCTGAG AGCTCCAACG CTATGCTTCC TCCTTTCCAA
    GAAACATCTT GTGATTTGCA GCAAAGAACT GAACCATCTC TTGGTCAACA GGCAAAGGAT
    AACCTCAATG TCGCTGCTCA GGGTGATACA TCAGTGGATG CGATCTACAA AGCAGTCGTA
    GATGCTGCAA GCAAAGGAAT GCAAGTAGTG ATCACCACTG CCGTTAGCAG TACCACACAG
    ATGAGTCCCA TTCCAGCTTT GAGTGCCATG AGTGCCTTCA CAGCCTCAAT TGGTGACCCG
    CTAAATCTCT CTAGTGCTGT CAGTGCAGTA ATCCATGGAA GAAACATTGC TGTTTCTGAT
    CATGAAGGTA GGATAAGAAA CACTAGAGGA ACGCGAGTAC TGAAGAATTC GGAGCATGGT
    AAAAATTCAA GTGAAGGGGA TGGGTATGAA TATTACAAAT CAACAAGTTG TAACACGCCC
    AAAAAACAGT GGGAAGGGGA GCAAAGTCCT GTCGGTGAGA TAAATAGGTG GAAATGTGAG
    GAGTTTCTAG ACCACTCCAC CCATATCCAT AGTAGTCCTT GTCACGAAAG GCCCAATAAC
    ATCTCCACAC TGCCATTATT GCAAGGTGAG CAGCATCAGA TACTGTTATC ACAGAGAAAC
    TGTCAAAGTG ATAAAATGTT GGAGGAGAAT TTCAGGTATA ACAATTACAA AAGAACTATG
    ATGAGTTTTA AGGAAAGACT GGAGAACACT GTGGAACGCT GTGCACACAT AAATGGAAAT
    AGGCCTCAGC AGAACAGAGG ATACGGGGAG TTGCTGAACA CTTCTAAACA AGACCTGATT
    CTGGAAGAGC AATCTCCAAG TTCCTCAAAT AGTTTGGAAA GTTCGTTAGT TAAAGACTAC
    ATCCATTACA ATGGAGATTT TAATGCCAAA AGCATTAATG GGTGTGTGCC TAGCCCTTCA
    GATGCTAAAA GCATCAGTAG TGAAGATGAC CTAAGGAATC CAGATTCCCC TTCTTCAAAT
    GAGCTGATAC ATTACAGGCC GAGGACGTTC AATGTTGGCG ACTTGGTCTG GGGCCAAATC
    AAAGGACTGA CTTCATGGCC TGGAAAACTA GTAAGAGAAG AAGACGTTCA CAATTCATGT
    CAACAGAACG CTGAGGAGGG GAAGGTGGAG CCAGAGAAGT TGAAGACACT AACAGAAGGT
    CTAGAAGCTT ACAACCGAGC CAGGAAAAGG AACAGAAAAA GTGGAAAGCT AAATAACCAT
    TTAGAAGCTG CTATACACGA GGCCATGAGT GAGCTAGACA AAATGTCTGG GAATGTCCAC
    CAAATCCCAC AGGGAGACAG ACAAGTGAAG CCTCCAAAAC CCAAGAGGAG GAAGATCTCT
    AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_422153.2
    CDS1366..5871
    Translation

    Target ORF information:

    RefSeq Version XM_422153.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus methyl-CpG binding domain protein 5 (MBD5), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_422153.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGTG GAAAGGAGTG TGACGGAGGG GACACGGATG GAGGGCCACC AGCAGTGCAA 
    GTTCCCGTTG GTTGGCAGCG ACGGGTGGAC CAAAGTGGAG TGCTCTATAT CAGTCCCAGT
    GGGTCTTTAT TATCCTGCTT GGAGCAGGTG AAGACCTACT TGCTGACTGA TGGAACCTGC
    AAATGTGGCC TAGAATGTCC TCTTGTTCTT CCCAAGGTCT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAGC AGAGAACTGC TGAAGATGTT AAAGCAGATG AAGATGTCAC CAAACTATGC
    ATACATAAAA GAAAGATTAT TGCTGTGGCT ACACTTCATA AAAGCATGGA AGCACCACAT
    CCTTCACTAG TTCTAACTAG TCCTGGTGGT GGAACAAGTG CAACTCCGGT AGTTCCTACT
    CGAGCAGCAA CTCCAAGATC AATGAGGAAT AAATCACATG AAGGAATTAC AAATTCTGTG
    ATGCCAGAAT GTAAGACTCC TTTCAAGTTG ATGATAGGGG CATCTAATGC CATGGGTAGG
    CTTTATGTGC AAGAAATGGC TGGAAGCCAG CAACAAGAAC TTCATTCTGT CTATCCTAGG
    CAGAGATTGG GTAGTAATGA ACTTGGACAG AAGTCTCCAT ATCGTGGCAG TCATGGTGGG
    ATGCCCAGTC CAGCTTCATC AGGATCACAG ATATATGGAG ACGGCTCAAT CTCTCCTAGG
    ACTGACCCAC TTGGAAGCCC TGATGTTTTC ACAAGGAACA ATCCCAGTTT TCATGGAGCA
    CCCAACTCAA GTCCTATTCA TATGAACAGG ACACCTCTGT CTCCGCCATC AGTAATGCTA
    CATGGTTCTC CCATACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CCACAAAAAG CCCTGTAATG AAAAAACCCA TGTGTAATTT TTCAGCTGGT
    ATGGAAATAC CACGAGCAAT GTTTCACCAT AAACCACCCC AAGGTCCACC TCCACCTCCT
    CCACCGCCTT CTTGTGCTCT TCAGAAAAAG CCATTAACAT CAGAAAAGGA TCCACTTGGC
    ATACTTGACC CTATTCCTAG CAAACCAGTC AATCAGAATC CTGTTATCAT TAACCCAACT
    ACTTTCCATT CAAATGTCCA CTCTCAGGTA CCCGTGATGA ATGTAAGCAT GCCTCCTGCT
    GTCGTCCCTT TGCCAAGTAA TCTTCCTTTG CCAACTGTAA AACCTGGTCA CGTGAACCAT
    GGAAGTCATG TTCAAAGAGT TCAGCATTCA GCTTCTACTT CCCTATCTCC TTCACCAGTG
    ACATCCCCAG TTCATATGAT GGGATCCGGG ATTGGAAGGA TAGAGGCTTC TCCCCAAAGA
    TCACGTTCTT CTTCCACATC ATCAGATCAT GGAAATTTCC TGCTGCCTCC AGTAGGACCG
    CAGTCATCTT GTACTGGTAT TAAAGTTCCT CCCAGGTCCC CACGGTCAAC AATAGGGTCG
    CCAAGACCGT CTATGCCATC TAGCCCCTCC ACCAAGCATG ATGGACTTAA TCAATACAAG
    GACATCCCTA ACCCATTAAT TGCTGGAATG AGTAATGTAT TAAATCCTCC AAACAATGCA
    GTTTTTTCTA CTGCATCGGC TGGAAGTGGT TCTTTGAAGA GTCAGCCTGG TTTGCTGGGA
    ATGCCTTTAA ATCAGATCTT GAACCAGCAC AATGCTGCCT CTTTTCCAGC AAGTAGTTTA
    CTCTCAGCAG CAGCCAAAGC ACAGCTAGCA AATCAAAATA AACTTGCTGG TAACAACAAT
    AACAGCAGTA GCAATTCTGG ACCTGTTGCC AGCGGTGGCA ACAACGAAGG ACATAGCACT
    TTAAATACCA TGTTCCCTCC TGCTGCCAAT GTGCTTCTAC CAACGACTGA AGGGCAAAGT
    GGTCGAGCAG CACTGAGAGA TAAACTGATG TCTCAGCAAA AAGATCCTCT GAGAAAAAGA
    AAGCAGCCAA CCACCACAGT GTTGAGTTTG CTGAGGCAGT CTCAGTTGGA GAGTTCTGGA
    GTTTCCAAAG CTGGATCTGA TTTAGTAAGA AAGCAAAGTC AAAGCTCTTT TCCCATCAGC
    TCTATGTCCC AATTACTTCA GTCCATGAGT TGTCAAAGCT CTCACATGAG CAGCAATAGT
    ACTACCAGCT GTGGGAGCTC AAACACTGTT TTACCTTGCT CTGGTAACCA GATGCATTTT
    GCAGACACCA GTATGAACTC TGGCACTCTT CAGAACTCAC TGGCACAGAG TTTACCATTA
    CGAGGGGAAG GTATGCACTG CCAGAACACA AACACTAACT TTGTCCATGG TACTAGCCCT
    GCCACCACCA ACCATCTCGC GGGTTTAATA AATCAGATGC AGGCTAGTGG GAACTGTGGG
    ATGCTCAGTC AGTCAGGAAT GGCTTTAGGA AATTCATTAC ATCCGAACCC ACCTCAGCCA
    AGAATCCAGG CATCCTCCAC TCCAGTGATA CCAAACAGCA TTGCTAGCAG CTGTAATCAA
    ACAAGTCCTG AAGCAGGGGG TTCAGGACCA TCATCATCAA TAGCCATAGC TGGCACCAGC
    CAACCTGCCA TCACAAAGAC AACATCTGTT CTTCAAGATG GTGTTATAGT CACTACTGCA
    GCTGGAAACC CTCTTCAGAG CCAGCTGCCC ATTGGGGGTG ATTTCCCTTT CGCTGGCCAC
    GAACACTCGC TTCATTTCCC GCAGAACAGC TCTTCAAACA ACAATCTTCC ACCTTCTTTG
    AATCAAAACC TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAGCA ACATCTCCTA
    AGCCAGAATC TATTAAATAT ACTGCAGCCT TCAGCAGGAG AAGGTGACAT GTCATCTATA
    AACACTACTT TGAATAACCA TCAACTGAGT CATCTCCAGT CACTATTAAA CAACAATCAG
    ATGTTTCCTT CAAATCAGCA GCAACAGCAC CTTCTCCAGG GGTATCAGAA CATGCAGGGC
    TTTCAAGGCC AGCCCCCAAT TCCTGGCCCA GCTAACAACC CAAACCCCAT GGCATGTCTG
    TTCCAAAATT TCCAGGTGAG AATGCAGGAA GATGCTGCTG CCCTGAACAA AAGAATGATC
    ACTCAGATGG GAATGGCACC AGTTCCTGAG AGCTCCAACG CTATGCTTCC TCCTTTCCAA
    GAAACATCTT GTGATTTGCA GCAAAGAACT GAACCATCTC TTGGTCAACA GGCAAAGGAT
    AACCTCAATG TCGCTGCTCA GGGTGATACA TCAGTGGATG CGATCTACAA AGCAGTCGTA
    GATGCTGCAA GCAAAGGAAT GCAAGTAGTG ATCACCACTG CCGTTAGCAG TACCACACAG
    ATGAGTCCCA TTCCAGCTTT GAGTGCCATG AGTGCCTTCA CAGCCTCAAT TGGTGACCCG
    CTAAATCTCT CTAGTGCTGT CAGTGCAGTA ATCCATGGAA GAAACATTGC TGTTTCTGAT
    CATGAAGGTA GGATAAGAAA CACTAGAGGA ACGCGAGTAC TGAAGAATTC GGAGCATGGT
    AAAAATTCAA GTGAAGGGGA TGGGTATGAA TATTACAAAT CAACAAGTTG TAACACGCCC
    AAAAAACAGT GGGAAGGGGA GCAAAGTCCT GTCGGTGAGA TAAATAGGTG GAAATGTGAG
    GAGTTTCTAG ACCACTCCAC CCATATCCAT AGTAGTCCTT GTCACGAAAG GCCCAATAAC
    ATCTCCACAC TGCCATTATT GCAAGGTGAG CAGCATCAGA TACTGTTATC ACAGAGAAAC
    TGTCAAAGTG ATAAAATGTT GGAGGAGAAT TTCAGGTATA ACAATTACAA AAGAACTATG
    ATGAGTTTTA AGGAAAGACT GGAGAACACT GTGGAACGCT GTGCACACAT AAATGGAAAT
    AGGCCTCAGC AGAACAGAGG ATACGGGGAG TTGCTGAACA CTTCTAAACA AGACCTGATT
    CTGGAAGAGC AATCTCCAAG TTCCTCAAAT AGTTTGGAAA GTTCGTTAGT TAAAGACTAC
    ATCCATTACA ATGGAGATTT TAATGCCAAA AGCATTAATG GGTGTGTGCC TAGCCCTTCA
    GATGCTAAAA GCATCAGTAG TGAAGATGAC CTAAGGAATC CAGATTCCCC TTCTTCAAAT
    GAGCTGATAC ATTACAGGCC GAGGACGTTC AATGTTGGCG ACTTGGTCTG GGGCCAAATC
    AAAGGACTGA CTTCATGGCC TGGAAAACTA GTAAGAGAAG AAGACGTTCA CAATTCATGT
    CAACAGAACG CTGAGGAGGG GAAGGTGGAG CCAGAGAAGT TGAAGACACT AACAGAAGGT
    CTAGAAGCTT ACAACCGAGC CAGGAAAAGG AACAGAAAAA GTGGAAAGCT AAATAACCAT
    TTAGAAGCTG CTATACACGA GGCCATGAGT GAGCTAGACA AAATGTCTGG GAATGTCCAC
    CAAATCCCAC AGGGAGACAG ACAAGTGAAG CCTCCAAAAC CCAAGAGGAG GAAGATCTCT
    AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.