F8 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	F8
Entrez Gene ID	100424151
Full Name	coagulation factor VIII
Gene Type	protein-coding
Organism	Macaca mulatta(Rhesus monkey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_028842499.1	XP_028698332.1	coagulation factor VIII isoform X3
XM_015128573.1	XP_014984059.1	coagulation factor VIII isoform X1
XM_015128573.2	XP_014984059.2	coagulation factor VIII isoform X1
XM_015128574.1	XP_014984060.1	coagulation factor VIII isoform X2
XM_015128574.2	XP_014984060.2	coagulation factor VIII isoform X2
XM_015128575.1	XP_014984061.1	coagulation factor VIII isoform X3
XM_028842500.1	XP_028698333.1	coagulation factor VIII isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	F8	NP_000123.1
Canis lupus familiaris (dog)	F8	NP_001003212.1
Pan troglodytes (chimpanzee)	F8	XP_003317837.1
	LOC100424151	XP_002806506.1
Rattus norvegicus (Norway rat)	F8	NP_899160.1
Mus musculus (house mouse)	F8	NP_032003.2
Bos taurus (cattle)	F8	NP_001138980.1

Gene Ontology

The following F8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMb94359	XM_028842499.1 Latest version!	Macaca mulatta coagulation factor VIII (F8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMb94360	XM_028842500.1 Latest version!	Macaca mulatta coagulation factor VIII (F8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMb56946	XM_015128575.1 Latest version!	Macaca mulatta coagulation factor VIII, procoagulant component (F8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMb56944	XM_015128573.1 Latest version!	Macaca mulatta coagulation factor VIII, procoagulant component (F8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb56945	XM_015128574.1 Latest version!	Macaca mulatta coagulation factor VIII, procoagulant component (F8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb94357	XM_015128573.2 Latest version!	Macaca mulatta coagulation factor VIII (F8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMb94358	XM_015128574.2 Latest version!	Macaca mulatta coagulation factor VIII (F8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F8 ( XM_028842499.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028698332.1 Macaca mulatta coagulation factor VIII (F8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMb94359
Clone ID Related Accession (Same CDS sequence)	XM_028842499.1
Accession Version	XM_028842499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	coagulation factor VIII isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATC CACTCTGGTT CATAGCAATC AATGGCTACA TAATGGATAC ACTACCTGGC 
TTAGTAATGG CTCAGGATCA AAGGATTCGA TGGTATCTGC TCAGCATGGG CAGCAATGAA 
AACATCCATT CTATTCATTT TAGTGGACAT GTGTTCACTG TACGAAAAAA AGAGGAGTAT 
AAAATGGCAG TGTACAATCT CTATCCAGGT GTTTTTGAGA CAGTTGAAAT GTTACCATCC 
AAAGCTGGAA TTTGGCGGGT GGAATGCCTT ATTGGCGAGC ACCTACATGC TGGGATGAGC 
ACACTTTTTC TGGTGTACAG CAACAAGTGT CAGACTCCCC TGGGAATGGC TTCTGGACGC 
ATTAGAGATT TTCAGATTAC AGCTTCAGGA CAATATGGAC AGTGGGCCCC AAAGCTGGCC 
AGACTTCATT ATTCTGGATC AATCAATGCC TGGAGCACCA AGGAGCCCTT TTCTTGGATC 
AAGGTGGATC TGTTGGCACC AATGATTATT CACGGCATCA AGACCCAGGG TGCCCGTCAG 
AAGTTCTCCA GCCTCTACAT CTCTCAGTTT ATCATCATGT ATAGTCTTGA TGGGAAAAAG 
TGGCAGACTT ATCGAGGAAA TTCCACTGGA ACCTTAATGG TCTTCTTTGG CAATGTGGAT 
TCATCTGGGA TAAAACACAA TATTTTTAAC CCTCCAATTA TTGCTCGATA CATCCGTTTG 
CACCCAACTC ATTATAGCAT TCGCAGCACT CTTCGCATGG AGTTGATGGG CTGTGATTTA 
AATAGTTGCA GCATGCCGTT GGGAATGGAG AGTAAAGCAA TATCAGATGC ACAGATTACT 
GCTTCATCCT ACTTCACCAA TATGTTTGCC ACCTGGTCTC CTTCAAAAGC TCGACTTAAC 
CTCCAAGGAA GGAGTAATGC CTGGAGACCT CAGGTGAATA ATCCAAAAGA GTGGCTGCAA 
GTGGACTTCC AGAAGACAAT GAAAGTCACA GGAATAACTA CTCAGGGAGT AAAATCTCTA 
CTTACCAACA TGTATGTGAA GGAGTTCCTC ATCTCCAGCA GTCAAGATGG CCATCACTGG 
ACTCTCTTCT TTCAGAATGG CAAAGTAAAG GTTTTTCAGG GAAATCAAGA CTCCTTCACA 
CCTGTGGTGA ACTCTCTAGA CTCACCGTTA CTGACTCGCT ACCTTCGAAT TCATCCCCAG 
AGTTGGGTGC ACCAGATTGC CCTGAGGATA GAGGTTCTGG GCTGCGAGGC ACAGGAGCTC 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028698332.1
CDS	169..1434
Translation	MRNPLWFIAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMAVYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLNLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTNMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDSPLLTRYLRIHPQSWVHQIALRIEVLGCEAQELY

Target ORF information:

RefSeq Version	XM_028842499.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta coagulation factor VIII (F8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028842499.1

ORF Insert Sequence:

ATGAGAAATC CACTCTGGTT CATAGCAATC AATGGCTACA TAATGGATAC ACTACCTGGC 
TTAGTAATGG CTCAGGATCA AAGGATTCGA TGGTATCTGC TCAGCATGGG CAGCAATGAA 
AACATCCATT CTATTCATTT TAGTGGACAT GTGTTCACTG TACGAAAAAA AGAGGAGTAT 
AAAATGGCAG TGTACAATCT CTATCCAGGT GTTTTTGAGA CAGTTGAAAT GTTACCATCC 
AAAGCTGGAA TTTGGCGGGT GGAATGCCTT ATTGGCGAGC ACCTACATGC TGGGATGAGC 
ACACTTTTTC TGGTGTACAG CAACAAGTGT CAGACTCCCC TGGGAATGGC TTCTGGACGC 
ATTAGAGATT TTCAGATTAC AGCTTCAGGA CAATATGGAC AGTGGGCCCC AAAGCTGGCC 
AGACTTCATT ATTCTGGATC AATCAATGCC TGGAGCACCA AGGAGCCCTT TTCTTGGATC 
AAGGTGGATC TGTTGGCACC AATGATTATT CACGGCATCA AGACCCAGGG TGCCCGTCAG 
AAGTTCTCCA GCCTCTACAT CTCTCAGTTT ATCATCATGT ATAGTCTTGA TGGGAAAAAG 
TGGCAGACTT ATCGAGGAAA TTCCACTGGA ACCTTAATGG TCTTCTTTGG CAATGTGGAT 
TCATCTGGGA TAAAACACAA TATTTTTAAC CCTCCAATTA TTGCTCGATA CATCCGTTTG 
CACCCAACTC ATTATAGCAT TCGCAGCACT CTTCGCATGG AGTTGATGGG CTGTGATTTA 
AATAGTTGCA GCATGCCGTT GGGAATGGAG AGTAAAGCAA TATCAGATGC ACAGATTACT 
GCTTCATCCT ACTTCACCAA TATGTTTGCC ACCTGGTCTC CTTCAAAAGC TCGACTTAAC 
CTCCAAGGAA GGAGTAATGC CTGGAGACCT CAGGTGAATA ATCCAAAAGA GTGGCTGCAA 
GTGGACTTCC AGAAGACAAT GAAAGTCACA GGAATAACTA CTCAGGGAGT AAAATCTCTA 
CTTACCAACA TGTATGTGAA GGAGTTCCTC ATCTCCAGCA GTCAAGATGG CCATCACTGG 
ACTCTCTTCT TTCAGAATGG CAAAGTAAAG GTTTTTCAGG GAAATCAAGA CTCCTTCACA 
CCTGTGGTGA ACTCTCTAGA CTCACCGTTA CTGACTCGCT ACCTTCGAAT TCATCCCCAG 
AGTTGGGTGC ACCAGATTGC CCTGAGGATA GAGGTTCTGG GCTGCGAGGC ACAGGAGCTC 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_028842500.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028698333.1 Macaca mulatta coagulation factor VIII (F8), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMb94360
Clone ID Related Accession (Same CDS sequence)	XM_028842500.1
Accession Version	XM_028842500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	coagulation factor VIII isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCC AAGACCCTGG GAAGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA 
CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT 
AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG 
CCGTTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTC 
ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTAACCTCCA AGGAAGGAGT 
AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG 
ACAATGAAAG TCACAGGAAT AACTACTCAG GGAGTAAAAT CTCTACTTAC CAACATGTAT 
GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC ACTGGACTCT CTTCTTTCAG 
AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT 
CTAGACTCAC CGTTACTGAC TCGCTACCTT CGAATTCATC CCCAGAGTTG GGTGCACCAG 
ATTGCCCTGA GGATAGAGGT TCTGGGCTGC GAGGCACAGG AGCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028698333.1
CDS	837..1487
Translation	MRSQDPGKVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLNLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTNMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDSPLLTRYLRIHPQSWVHQIALRIEVLGCEAQELY

Target ORF information:

RefSeq Version	XM_028842500.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta coagulation factor VIII (F8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028842500.1

ORF Insert Sequence:

ATGCGGAGCC AAGACCCTGG GAAGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA 
CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT 
AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG 
CCGTTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTC 
ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTAACCTCCA AGGAAGGAGT 
AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG 
ACAATGAAAG TCACAGGAAT AACTACTCAG GGAGTAAAAT CTCTACTTAC CAACATGTAT 
GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC ACTGGACTCT CTTCTTTCAG 
AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT 
CTAGACTCAC CGTTACTGAC TCGCTACCTT CGAATTCATC CCCAGAGTTG GGTGCACCAG 
ATTGCCCTGA GGATAGAGGT TCTGGGCTGC GAGGCACAGG AGCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_015128575.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014984061.1 Macaca mulatta coagulation factor VIII, procoagulant component (F8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMb56946
Clone ID Related Accession (Same CDS sequence)	XM_015128575.1
Accession Version	XM_015128575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	coagulation factor VIII isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014806045.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAATAG AGCTCTCCAC CTACTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC 
GGTGAGCTAC CTGTGGACAC AAGATTTCCT CCTAGAGTGC CAAGATCTTT TCCATTCAAC 
ACCTCAGTCA TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC 
GCTAAGCCAA GGCCACCTTG GATGGGTCTG CTGGGTCCTA CTATCCAGGC TGAGGTTTAT 
GACACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT 
GGTGTTTCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG 
GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG 
AAAGAGAATG GTCCAATGGC CTCTGACCCG CTGTGCCTTA CCTACTCGTA TCTTTCTCAT 
GTGGACCTGG TAAAAGACTT GAATTCGGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA 
GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTGTACTACT TTTTGCTGTA 
TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT 
GATGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT 
CTACCAGGTC TGATTGGATG CCACAGGAAG TCAGTCTATT GGCATGTGAT TGGAATGGGC 
ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT 
CGCCAGGCCT CCTTGGAAAT CTCACCAATA ACTTTCCTTA CTGCTCAAAC ACTCCTGATG 
GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA 
GCTTATGTCA AAGTAGACAG TTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA 
GAAGCGGAAG ACTATGATGA TGATCTTGCT GATTCTGAAA TGGATGTGGT CAGGTTTGAT 
GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT 
TGGGTACATT ACATTGCTGC TGAAGAGGAG GTCTGGGACT ATGCTCCCTC AGTCCTCGCC 
CCTGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCTCTCAGCG GATTGGTAGG 
AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT 
ATTCAGTATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG 
TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT 
GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT 
CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA 
ACTAAATCAG ATCCTCGGTG CCTGACCCGT TATTACTCTA GTTTCATTAA TATGGAGAGA 
GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA 
AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT TTTTGATGAG 
AACCAAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGTTGGAGTG 
CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT 
TTTGATAGTC TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC 
ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA 
ATGGTCTATG AAGACACACT TACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAAC ATACTTGCTG AGTAAAAACA ATGCCATTGA GCCAAGAAGT 
TTCTCCCAGA ATTCAAGACA CCCTAGCCCT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCCAAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC AATGCCTAAA 
GTACAGAATG TCTCCTCTAG TGATTTGCTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC GAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACACTAA TAACAACCTT TCTAAAATGA CACACCTCAG GCCACAGCCC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGCCAGACC TCCAATTAAG ATTAAATGAG 
AAACTGGGGA CAACTGTAGC AACAGAGTTG AAAAAATTTG ATTTTAAAGT TTCTAGTTCA 
TCAAATAATC TGATTTCAAC AATTCCGTCA GACAATTTGG TAGCAGGTAG TGATAATACA 
AGTTCCTTAG GACCCCCAAA TATACCAGTT CATTATGAGA GTCAATTAGA TACCACTCTG 
TCTGGCAAAA AGTCATCTCC CCTTATTGAG TCTGGTGGAC CTCTGAGTTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT GGTCAACAGA GAGTGGTAGG TTATTTAAAG AGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGATAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
GTTGAGAATA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGATACTGA ATTTCAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ACAGACAAAA ATACTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCG AAAAACATGG AAATGGTCCA ACAAAAAATA 
GAGGGCCCCA TACTACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTACCAGAAT CAGCAAATTG GATACAAAGG ACTCATGGAA AGAACTCCCT GAACTCGGGG 
CAAGGCCCCA GTGCAAAACT ATTCATATCC TTAGGACCAG AAAACTCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA TAAAGTGGTA GTAGGAAAGG GTGAACTTAC AAAGGATATA 
GGACTCAAAG AGATAGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGAAAG 
GAAACATTAA TCCAAGATAA TATAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA GTGATTCAAC AAATAGAACA 
AAGAACCACA TGGCTCATTT CTCAGAAAAA GGGGAGGAAG AAAACTTGGA AGGTTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT CCACACACCA CAAGGATATC TCCTAATCCA 
AACCAGCAAA ATTTTGTCAC GCAACGTGGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGCTA ATTGTGGAGG AGACCTCAAC CCAGTGGTCC 
AAAAACATAA AACATTTGAC CCCAAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGAGCCA TTACTCAGTC TCCCTTATCA GATTGTCTTA CGAGGAGTCA TAGCATCACT 
CAAGCAAATA GATCTCCATT ACCCATTGCA AAGGTATCAT CATTTCCATC TATTAGACCT 
ATGGATCTGA CCAGGGTCCT ATTCCAAGAC AACTTTTCTC GTCTTCCAGC ACCACCTTAC 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGT CAAAAAAAAT 
AACCTTTCTT TAGCTATTCT AACCTTGGAG ATGATTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGTGACAA GTGCTACAAA TTCAGTCACG TACAAGAAAG TTGAGAACAC TGTTTTCTTG 
AAACCAGGCT TGCCCGAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCGCATTTAT 
CAGAAGGACC TTTTCCCTAC AGAAACTAGC AGTGGGTCTC CTGGCCATCT GGATCTCATG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCAATTAAGT GGAAGGAAGC AAATAGACCT 
GGAAAAATTC CCTTTCTGAG AGGAGCAACA GAAAGCTCTG CAAAGACTCC TTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAACACAGCT TTTAAGAAAA AGGATACCAT TTTGCCCCTG 
AACCCTTGTG AAAGCAATCA TACAATAGCA GCAATAAATG AGGAACAAAA TGAGCCCCAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTGGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GTCTTGAAAC GTCATCAAAG GGAAATAACT CTTAATACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGGT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGC GGCTCTGGGA TTATGGGATG AGTAGTTCCC CGCATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGAAAAA ATTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGAAAT GTCATTCTGG AATCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014984061.1
CDS	434..6043
Translation	MQIELSTYFFLCLLRFCFSATRRYYLGAVELSWDYMQSDLGELPVDTRFPPRVPRSFPFNTSVMYKKTVFVEFTDHLFNIAKPRPPWMGLLGPTIQAEVYDTVVITLKNMASHPVSLHAVGVSYWKASEGAEYDDQTSQREKEDDKVFPGGSHTYVWQVLKENGPMASDPLCLTYSYLSHVDLVKDLNSGLIGALLVCREGSLAKEKTQTLHKFVLLFAVFDEGKSWHSETKNSLMQDRDDASARAWPKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTLLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKNNEEAEDYDDDLADSEMDVVRFDDDNSPSFIQIRSVAKKHPKTWVHYIAAEEEVWDYAPSVLAPDDRSYKSQYLNNGSQRIGRKYKKVRFMAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVRPLYSRRLPKGVKHLKDFPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINMERDLASGLIGPLLICYKESVDQRGNQIMSDKRNVILFSVFDENQSWYLTENIQRFLPNPVGVQLEDPEFQASNIMHSINGYVFDSLQLSVCLHEVAYWYILSIGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISTYLLSKNNAIEPRSFSQNSRHPSPRQKQFNATTIPKNDIEKTDPWFAHRTPMPKVQNVSSSDLLMLLRQSPTPHGLSLSDLQEAEYETFSDDPSPGAIDTNNNLSKMTHLRPQPHHSGDMVFTPEPDLQLRLNEKLGTTVATELKKFDFKVSSSSNNLISTIPSDNLVAGSDNTSSLGPPNIPVHYESQLDTTLSGKKSSPLIESGGPLSLSEENNDSKLLESGLMNSQESSWGKNVWSTESGRLFKEKRAHGPALLTKDNALFKVSISLLKINKTSNNSATNRKTHIDGPSLLVENSPSVWQNILESDTEFQKVTPLIHDRMLTDKNTTALRLNHMSNKTTSSKNMEMVQQKIEGPILPDAENPDMSFFKMLFLPESANWIQRTHGKNSLNSGQGPSAKLFISLGPENSVEGQNFLSEKNKVVVGKGELTKDIGLKEIVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIERKETLIQDNIVLPQIHTVTGTKNFMKNLFLLSTRQNVEGSYEGAYAPVLQDFRSLSDSTNRTKNHMAHFSEKGEEENLEGLGNQTKQIVEKYPHTTRISPNPNQQNFVTQRGKRALKQFRLPLEETELEKRLIVEETSTQWSKNIKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSITQANRSPLPIAKVSSFPSIRPMDLTRVLFQDNFSRLPAPPYRKKDSGVQESSHFLQGVKKNNLSLAILTLEMIGDQREVGSLVTSATNSVTYKKVENTVFLKPGLPETSGKVELLPKVRIYQKDLFPTETSSGSPGHLDLMEGSLLQETEGAIKWKEANRPGKIPFLRGATESSAKTPSKLLDPLAWDNHYGTQIPKEEWKSQEKSPENTAFKKKDTILPLNPCESNHTIAAINEEQNEPQIEVTWAKQGGTERLCSQNPPVLKRHQREITLNTLQSDQEEIDYDDTISVEMKKEDFDIYGEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSGSVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLKCHSGIY

Target ORF information:

RefSeq Version	XM_015128575.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta coagulation factor VIII, procoagulant component (F8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015128575.1

ORF Insert Sequence:

ATGCAAATAG AGCTCTCCAC CTACTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC 
GGTGAGCTAC CTGTGGACAC AAGATTTCCT CCTAGAGTGC CAAGATCTTT TCCATTCAAC 
ACCTCAGTCA TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC 
GCTAAGCCAA GGCCACCTTG GATGGGTCTG CTGGGTCCTA CTATCCAGGC TGAGGTTTAT 
GACACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT 
GGTGTTTCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG 
GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG 
AAAGAGAATG GTCCAATGGC CTCTGACCCG CTGTGCCTTA CCTACTCGTA TCTTTCTCAT 
GTGGACCTGG TAAAAGACTT GAATTCGGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA 
GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTGTACTACT TTTTGCTGTA 
TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT 
GATGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT 
CTACCAGGTC TGATTGGATG CCACAGGAAG TCAGTCTATT GGCATGTGAT TGGAATGGGC 
ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT 
CGCCAGGCCT CCTTGGAAAT CTCACCAATA ACTTTCCTTA CTGCTCAAAC ACTCCTGATG 
GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA 
GCTTATGTCA AAGTAGACAG TTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA 
GAAGCGGAAG ACTATGATGA TGATCTTGCT GATTCTGAAA TGGATGTGGT CAGGTTTGAT 
GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT 
TGGGTACATT ACATTGCTGC TGAAGAGGAG GTCTGGGACT ATGCTCCCTC AGTCCTCGCC 
CCTGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCTCTCAGCG GATTGGTAGG 
AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT 
ATTCAGTATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG 
TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT 
GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT 
CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA 
ACTAAATCAG ATCCTCGGTG CCTGACCCGT TATTACTCTA GTTTCATTAA TATGGAGAGA 
GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA 
AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT TTTTGATGAG 
AACCAAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGTTGGAGTG 
CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT 
TTTGATAGTC TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC 
ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA 
ATGGTCTATG AAGACACACT TACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAAC ATACTTGCTG AGTAAAAACA ATGCCATTGA GCCAAGAAGT 
TTCTCCCAGA ATTCAAGACA CCCTAGCCCT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCCAAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC AATGCCTAAA 
GTACAGAATG TCTCCTCTAG TGATTTGCTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC GAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACACTAA TAACAACCTT TCTAAAATGA CACACCTCAG GCCACAGCCC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGCCAGACC TCCAATTAAG ATTAAATGAG 
AAACTGGGGA CAACTGTAGC AACAGAGTTG AAAAAATTTG ATTTTAAAGT TTCTAGTTCA 
TCAAATAATC TGATTTCAAC AATTCCGTCA GACAATTTGG TAGCAGGTAG TGATAATACA 
AGTTCCTTAG GACCCCCAAA TATACCAGTT CATTATGAGA GTCAATTAGA TACCACTCTG 
TCTGGCAAAA AGTCATCTCC CCTTATTGAG TCTGGTGGAC CTCTGAGTTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT GGTCAACAGA GAGTGGTAGG TTATTTAAAG AGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGATAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
GTTGAGAATA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGATACTGA ATTTCAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ACAGACAAAA ATACTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCG AAAAACATGG AAATGGTCCA ACAAAAAATA 
GAGGGCCCCA TACTACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTACCAGAAT CAGCAAATTG GATACAAAGG ACTCATGGAA AGAACTCCCT GAACTCGGGG 
CAAGGCCCCA GTGCAAAACT ATTCATATCC TTAGGACCAG AAAACTCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA TAAAGTGGTA GTAGGAAAGG GTGAACTTAC AAAGGATATA 
GGACTCAAAG AGATAGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGAAAG 
GAAACATTAA TCCAAGATAA TATAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA GTGATTCAAC AAATAGAACA 
AAGAACCACA TGGCTCATTT CTCAGAAAAA GGGGAGGAAG AAAACTTGGA AGGTTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT CCACACACCA CAAGGATATC TCCTAATCCA 
AACCAGCAAA ATTTTGTCAC GCAACGTGGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGCTA ATTGTGGAGG AGACCTCAAC CCAGTGGTCC 
AAAAACATAA AACATTTGAC CCCAAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGAGCCA TTACTCAGTC TCCCTTATCA GATTGTCTTA CGAGGAGTCA TAGCATCACT 
CAAGCAAATA GATCTCCATT ACCCATTGCA AAGGTATCAT CATTTCCATC TATTAGACCT 
ATGGATCTGA CCAGGGTCCT ATTCCAAGAC AACTTTTCTC GTCTTCCAGC ACCACCTTAC 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGT CAAAAAAAAT 
AACCTTTCTT TAGCTATTCT AACCTTGGAG ATGATTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGTGACAA GTGCTACAAA TTCAGTCACG TACAAGAAAG TTGAGAACAC TGTTTTCTTG 
AAACCAGGCT TGCCCGAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCGCATTTAT 
CAGAAGGACC TTTTCCCTAC AGAAACTAGC AGTGGGTCTC CTGGCCATCT GGATCTCATG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCAATTAAGT GGAAGGAAGC AAATAGACCT 
GGAAAAATTC CCTTTCTGAG AGGAGCAACA GAAAGCTCTG CAAAGACTCC TTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAACACAGCT TTTAAGAAAA AGGATACCAT TTTGCCCCTG 
AACCCTTGTG AAAGCAATCA TACAATAGCA GCAATAAATG AGGAACAAAA TGAGCCCCAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTGGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GTCTTGAAAC GTCATCAAAG GGAAATAACT CTTAATACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGGT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGC GGCTCTGGGA TTATGGGATG AGTAGTTCCC CGCATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGAAAAA ATTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGAAAT GTCATTCTGG AATCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_015128573.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014984059.1 Macaca mulatta coagulation factor VIII, procoagulant component (F8), transcript variant X1, mRNA.

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CloneID	OMb56944
Clone ID Related Accession (Same CDS sequence)	XM_015128573.1
Accession Version	XM_015128573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	coagulation factor VIII isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014806045.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAATAG AGCTCTCCAC CTACTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC 
GGTGAGCTAC CTGTGGACAC AAGATTTCCT CCTAGAGTGC CAAGATCTTT TCCATTCAAC 
ACCTCAGTCA TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC 
GCTAAGCCAA GGCCACCTTG GATGGGTCTG CTGGGTCCTA CTATCCAGGC TGAGGTTTAT 
GACACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT 
GGTGTTTCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG 
GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG 
AAAGAGAATG GTCCAATGGC CTCTGACCCG CTGTGCCTTA CCTACTCGTA TCTTTCTCAT 
GTGGACCTGG TAAAAGACTT GAATTCGGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA 
GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTGTACTACT TTTTGCTGTA 
TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT 
GATGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT 
CTACCAGGTC TGATTGGATG CCACAGGAAG TCAGTCTATT GGCATGTGAT TGGAATGGGC 
ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT 
CGCCAGGCCT CCTTGGAAAT CTCACCAATA ACTTTCCTTA CTGCTCAAAC ACTCCTGATG 
GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA 
GCTTATGTCA AAGTAGACAG TTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA 
GAAGCGGAAG ACTATGATGA TGATCTTGCT GATTCTGAAA TGGATGTGGT CAGGTTTGAT 
GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT 
TGGGTACATT ACATTGCTGC TGAAGAGGAG GTCTGGGACT ATGCTCCCTC AGTCCTCGCC 
CCTGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCTCTCAGCG GATTGGTAGG 
AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT 
ATTCAGTATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG 
TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT 
GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT 
CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA 
ACTAAATCAG ATCCTCGGTG CCTGACCCGT TATTACTCTA GTTTCATTAA TATGGAGAGA 
GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA 
AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT TTTTGATGAG 
AACCAAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGTTGGAGTG 
CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT 
TTTGATAGTC TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC 
ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA 
ATGGTCTATG AAGACACACT TACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAAC ATACTTGCTG AGTAAAAACA ATGCCATTGA GCCAAGAAGT 
TTCTCCCAGA ATTCAAGACA CCCTAGCCCT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCCAAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC AATGCCTAAA 
GTACAGAATG TCTCCTCTAG TGATTTGCTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC GAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACACTAA TAACAACCTT TCTAAAATGA CACACCTCAG GCCACAGCCC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGCCAGACC TCCAATTAAG ATTAAATGAG 
AAACTGGGGA CAACTGTAGC AACAGAGTTG AAAAAATTTG ATTTTAAAGT TTCTAGTTCA 
TCAAATAATC TGATTTCAAC AATTCCGTCA GACAATTTGG TAGCAGGTAG TGATAATACA 
AGTTCCTTAG GACCCCCAAA TATACCAGTT CATTATGAGA GTCAATTAGA TACCACTCTG 
TCTGGCAAAA AGTCATCTCC CCTTATTGAG TCTGGTGGAC CTCTGAGTTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT GGTCAACAGA GAGTGGTAGG TTATTTAAAG AGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGATAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
GTTGAGAATA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGATACTGA ATTTCAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ACAGACAAAA ATACTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCG AAAAACATGG AAATGGTCCA ACAAAAAATA 
GAGGGCCCCA TACTACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTACCAGAAT CAGCAAATTG GATACAAAGG ACTCATGGAA AGAACTCCCT GAACTCGGGG 
CAAGGCCCCA GTGCAAAACT ATTCATATCC TTAGGACCAG AAAACTCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA TAAAGTGGTA GTAGGAAAGG GTGAACTTAC AAAGGATATA 
GGACTCAAAG AGATAGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGAAAG 
GAAACATTAA TCCAAGATAA TATAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA GTGATTCAAC AAATAGAACA 
AAGAACCACA TGGCTCATTT CTCAGAAAAA GGGGAGGAAG AAAACTTGGA AGGTTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT CCACACACCA CAAGGATATC TCCTAATCCA 
AACCAGCAAA ATTTTGTCAC GCAACGTGGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGCTA ATTGTGGAGG AGACCTCAAC CCAGTGGTCC 
AAAAACATAA AACATTTGAC CCCAAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGAGCCA TTACTCAGTC TCCCTTATCA GATTGTCTTA CGAGGAGTCA TAGCATCACT 
CAAGCAAATA GATCTCCATT ACCCATTGCA AAGGTATCAT CATTTCCATC TATTAGACCT 
ATGGATCTGA CCAGGGTCCT ATTCCAAGAC AACTTTTCTC GTCTTCCAGC ACCACCTTAC 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGT CAAAAAAAAT 
AACCTTTCTT TAGCTATTCT AACCTTGGAG ATGATTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGTGACAA GTGCTACAAA TTCAGTCACG TACAAGAAAG TTGAGAACAC TGTTTTCTTG 
AAACCAGGCT TGCCCGAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCGCATTTAT 
CAGAAGGACC TTTTCCCTAC AGAAACTAGC AGTGGGTCTC CTGGCCATCT GGATCTCATG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCAATTAAGT GGAAGGAAGC AAATAGACCT 
GGAAAAATTC CCTTTCTGAG AGGAGCAACA GAAAGCTCTG CAAAGACTCC TTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAACACAGCT TTTAAGAAAA AGGATACCAT TTTGCCCCTG 
AACCCTTGTG AAAGCAATCA TACAATAGCA GCAATAAATG AGGAACAAAA TGAGCCCCAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTGGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GTCTTGAAAC GTCATCAAAG GGAAATAACT CTTAATACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGGT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGC GGCTCTGGGA TTATGGGATG AGTAGTTCCC CGCATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGAAAAA ATTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC 
ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACT 
ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA CAGAAAGAAA CTGCAGGGCT 
CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC 
AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA 
TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT TAGTGGACAT 
GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAG TGTACAATCT CTATCCAGGT 
GTTTTTGAGA CAGTTGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT 
ATTGGCGAGC ACCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAACAAGTGT 
CAGACTCCCC TGGGAATGGC TTCTGGACGC ATTAGAGATT TTCAGATTAC AGCTTCAGGA 
CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCTGGATC AATCAATGCC 
TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT 
CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT 
ATCATCATGT ATAGTCTTGA TGGGAAAAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA 
ACCTTAATGG TATGTAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014984059.1
CDS	433..6873
Translation	MQIELSTYFFLCLLRFCFSATRRYYLGAVELSWDYMQSDLGELPVDTRFPPRVPRSFPFNTSVMYKKTVFVEFTDHLFNIAKPRPPWMGLLGPTIQAEVYDTVVITLKNMASHPVSLHAVGVSYWKASEGAEYDDQTSQREKEDDKVFPGGSHTYVWQVLKENGPMASDPLCLTYSYLSHVDLVKDLNSGLIGALLVCREGSLAKEKTQTLHKFVLLFAVFDEGKSWHSETKNSLMQDRDDASARAWPKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTLLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKNNEEAEDYDDDLADSEMDVVRFDDDNSPSFIQIRSVAKKHPKTWVHYIAAEEEVWDYAPSVLAPDDRSYKSQYLNNGSQRIGRKYKKVRFMAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVRPLYSRRLPKGVKHLKDFPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINMERDLASGLIGPLLICYKESVDQRGNQIMSDKRNVILFSVFDENQSWYLTENIQRFLPNPVGVQLEDPEFQASNIMHSINGYVFDSLQLSVCLHEVAYWYILSIGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISTYLLSKNNAIEPRSFSQNSRHPSPRQKQFNATTIPKNDIEKTDPWFAHRTPMPKVQNVSSSDLLMLLRQSPTPHGLSLSDLQEAEYETFSDDPSPGAIDTNNNLSKMTHLRPQPHHSGDMVFTPEPDLQLRLNEKLGTTVATELKKFDFKVSSSSNNLISTIPSDNLVAGSDNTSSLGPPNIPVHYESQLDTTLSGKKSSPLIESGGPLSLSEENNDSKLLESGLMNSQESSWGKNVWSTESGRLFKEKRAHGPALLTKDNALFKVSISLLKINKTSNNSATNRKTHIDGPSLLVENSPSVWQNILESDTEFQKVTPLIHDRMLTDKNTTALRLNHMSNKTTSSKNMEMVQQKIEGPILPDAENPDMSFFKMLFLPESANWIQRTHGKNSLNSGQGPSAKLFISLGPENSVEGQNFLSEKNKVVVGKGELTKDIGLKEIVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIERKETLIQDNIVLPQIHTVTGTKNFMKNLFLLSTRQNVEGSYEGAYAPVLQDFRSLSDSTNRTKNHMAHFSEKGEEENLEGLGNQTKQIVEKYPHTTRISPNPNQQNFVTQRGKRALKQFRLPLEETELEKRLIVEETSTQWSKNIKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSITQANRSPLPIAKVSSFPSIRPMDLTRVLFQDNFSRLPAPPYRKKDSGVQESSHFLQGVKKNNLSLAILTLEMIGDQREVGSLVTSATNSVTYKKVENTVFLKPGLPETSGKVELLPKVRIYQKDLFPTETSSGSPGHLDLMEGSLLQETEGAIKWKEANRPGKIPFLRGATESSAKTPSKLLDPLAWDNHYGTQIPKEEWKSQEKSPENTAFKKKDTILPLNPCESNHTIAAINEEQNEPQIEVTWAKQGGTERLCSQNPPVLKRHQREITLNTLQSDQEEIDYDDTISVEMKKEDFDIYGEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSGSVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFDETKSWYFTENTERNCRAPCNIQMEDPTFKENYRFHAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMAVYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVCN

Target ORF information:

RefSeq Version	XM_015128573.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta coagulation factor VIII, procoagulant component (F8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015128573.1

ORF Insert Sequence:

ATGCAAATAG AGCTCTCCAC CTACTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC 
GGTGAGCTAC CTGTGGACAC AAGATTTCCT CCTAGAGTGC CAAGATCTTT TCCATTCAAC 
ACCTCAGTCA TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC 
GCTAAGCCAA GGCCACCTTG GATGGGTCTG CTGGGTCCTA CTATCCAGGC TGAGGTTTAT 
GACACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT 
GGTGTTTCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG 
GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG 
AAAGAGAATG GTCCAATGGC CTCTGACCCG CTGTGCCTTA CCTACTCGTA TCTTTCTCAT 
GTGGACCTGG TAAAAGACTT GAATTCGGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA 
GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTGTACTACT TTTTGCTGTA 
TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT 
GATGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT 
CTACCAGGTC TGATTGGATG CCACAGGAAG TCAGTCTATT GGCATGTGAT TGGAATGGGC 
ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT 
CGCCAGGCCT CCTTGGAAAT CTCACCAATA ACTTTCCTTA CTGCTCAAAC ACTCCTGATG 
GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA 
GCTTATGTCA AAGTAGACAG TTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA 
GAAGCGGAAG ACTATGATGA TGATCTTGCT GATTCTGAAA TGGATGTGGT CAGGTTTGAT 
GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT 
TGGGTACATT ACATTGCTGC TGAAGAGGAG GTCTGGGACT ATGCTCCCTC AGTCCTCGCC 
CCTGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCTCTCAGCG GATTGGTAGG 
AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT 
ATTCAGTATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG 
TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT 
GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT 
CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA 
ACTAAATCAG ATCCTCGGTG CCTGACCCGT TATTACTCTA GTTTCATTAA TATGGAGAGA 
GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA 
AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT TTTTGATGAG 
AACCAAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGTTGGAGTG 
CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT 
TTTGATAGTC TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC 
ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA 
ATGGTCTATG AAGACACACT TACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAAC ATACTTGCTG AGTAAAAACA ATGCCATTGA GCCAAGAAGT 
TTCTCCCAGA ATTCAAGACA CCCTAGCCCT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCCAAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC AATGCCTAAA 
GTACAGAATG TCTCCTCTAG TGATTTGCTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC GAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACACTAA TAACAACCTT TCTAAAATGA CACACCTCAG GCCACAGCCC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGCCAGACC TCCAATTAAG ATTAAATGAG 
AAACTGGGGA CAACTGTAGC AACAGAGTTG AAAAAATTTG ATTTTAAAGT TTCTAGTTCA 
TCAAATAATC TGATTTCAAC AATTCCGTCA GACAATTTGG TAGCAGGTAG TGATAATACA 
AGTTCCTTAG GACCCCCAAA TATACCAGTT CATTATGAGA GTCAATTAGA TACCACTCTG 
TCTGGCAAAA AGTCATCTCC CCTTATTGAG TCTGGTGGAC CTCTGAGTTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT GGTCAACAGA GAGTGGTAGG TTATTTAAAG AGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGATAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
GTTGAGAATA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGATACTGA ATTTCAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ACAGACAAAA ATACTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCG AAAAACATGG AAATGGTCCA ACAAAAAATA 
GAGGGCCCCA TACTACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTACCAGAAT CAGCAAATTG GATACAAAGG ACTCATGGAA AGAACTCCCT GAACTCGGGG 
CAAGGCCCCA GTGCAAAACT ATTCATATCC TTAGGACCAG AAAACTCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA TAAAGTGGTA GTAGGAAAGG GTGAACTTAC AAAGGATATA 
GGACTCAAAG AGATAGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGAAAG 
GAAACATTAA TCCAAGATAA TATAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA GTGATTCAAC AAATAGAACA 
AAGAACCACA TGGCTCATTT CTCAGAAAAA GGGGAGGAAG AAAACTTGGA AGGTTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT CCACACACCA CAAGGATATC TCCTAATCCA 
AACCAGCAAA ATTTTGTCAC GCAACGTGGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGCTA ATTGTGGAGG AGACCTCAAC CCAGTGGTCC 
AAAAACATAA AACATTTGAC CCCAAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGAGCCA TTACTCAGTC TCCCTTATCA GATTGTCTTA CGAGGAGTCA TAGCATCACT 
CAAGCAAATA GATCTCCATT ACCCATTGCA AAGGTATCAT CATTTCCATC TATTAGACCT 
ATGGATCTGA CCAGGGTCCT ATTCCAAGAC AACTTTTCTC GTCTTCCAGC ACCACCTTAC 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGT CAAAAAAAAT 
AACCTTTCTT TAGCTATTCT AACCTTGGAG ATGATTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGTGACAA GTGCTACAAA TTCAGTCACG TACAAGAAAG TTGAGAACAC TGTTTTCTTG 
AAACCAGGCT TGCCCGAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCGCATTTAT 
CAGAAGGACC TTTTCCCTAC AGAAACTAGC AGTGGGTCTC CTGGCCATCT GGATCTCATG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCAATTAAGT GGAAGGAAGC AAATAGACCT 
GGAAAAATTC CCTTTCTGAG AGGAGCAACA GAAAGCTCTG CAAAGACTCC TTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAACACAGCT TTTAAGAAAA AGGATACCAT TTTGCCCCTG 
AACCCTTGTG AAAGCAATCA TACAATAGCA GCAATAAATG AGGAACAAAA TGAGCCCCAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTGGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GTCTTGAAAC GTCATCAAAG GGAAATAACT CTTAATACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGGT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGC GGCTCTGGGA TTATGGGATG AGTAGTTCCC CGCATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGAAAAA ATTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC 
ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACT 
ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA CAGAAAGAAA CTGCAGGGCT 
CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC 
AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA 
TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT TAGTGGACAT 
GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAG TGTACAATCT CTATCCAGGT 
GTTTTTGAGA CAGTTGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT 
ATTGGCGAGC ACCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAACAAGTGT 
CAGACTCCCC TGGGAATGGC TTCTGGACGC ATTAGAGATT TTCAGATTAC AGCTTCAGGA 
CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCTGGATC AATCAATGCC 
TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT 
CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT 
ATCATCATGT ATAGTCTTGA TGGGAAAAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA 
ACCTTAATGG TATGTAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_015128574.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014984060.1 Macaca mulatta coagulation factor VIII, procoagulant component (F8), transcript variant X2, mRNA.

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CloneID	OMb56945
Clone ID Related Accession (Same CDS sequence)	XM_015128574.1
Accession Version	XM_015128574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	coagulation factor VIII isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014806045.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGTG ATCTCGGTGA GCTACCTGTG GACACAAGAT TTCCTCCTAG AGTGCCAAGA 
TCTTTTCCAT TCAACACCTC AGTCATGTAC AAAAAGACTG TGTTTGTAGA ATTCACGGAT 
CACCTTTTCA ACATCGCTAA GCCAAGGCCA CCTTGGATGG GTCTGCTGGG TCCTACTATC 
CAGGCTGAGG TTTATGACAC AGTGGTCATT ACACTTAAGA ACATGGCTTC CCATCCTGTC 
AGTCTTCATG CTGTTGGTGT TTCCTACTGG AAAGCTTCTG AGGGAGCTGA ATATGATGAT 
CAGACCAGTC AAAGGGAGAA AGAAGATGAT AAAGTCTTTC CTGGTGGAAG CCATACATAT 
GTCTGGCAGG TCCTGAAAGA GAATGGTCCA ATGGCCTCTG ACCCGCTGTG CCTTACCTAC 
TCGTATCTTT CTCATGTGGA CCTGGTAAAA GACTTGAATT CGGGCCTCAT TGGAGCCCTA 
CTAGTATGTA GAGAAGGGAG TCTGGCCAAG GAAAAGACAC AGACCTTGCA CAAATTTGTA 
CTACTTTTTG CTGTATTTGA TGAAGGGAAA AGTTGGCACT CAGAAACAAA GAACTCCTTG 
ATGCAGGATA GGGATGATGC ATCTGCTCGG GCCTGGCCTA AAATGCACAC AGTCAATGGT 
TATGTAAACA GGTCTCTACC AGGTCTGATT GGATGCCACA GGAAGTCAGT CTATTGGCAT 
GTGATTGGAA TGGGCACCAC TCCTGAAGTG CACTCAATAT TCCTCGAAGG TCACACATTT 
CTTGTGAGGA ACCATCGCCA GGCCTCCTTG GAAATCTCAC CAATAACTTT CCTTACTGCT 
CAAACACTCC TGATGGACCT TGGACAGTTT CTACTGTTTT GTCATATCTC TTCCCACCAA 
CATGATGGCA TGGAAGCTTA TGTCAAAGTA GACAGTTGCC CAGAGGAACC CCAACTACGA 
ATGAAAAATA ATGAAGAAGC GGAAGACTAT GATGATGATC TTGCTGATTC TGAAATGGAT 
GTGGTCAGGT TTGATGATGA CAACTCTCCT TCCTTTATCC AAATTCGCTC AGTTGCCAAG 
AAGCATCCTA AAACTTGGGT ACATTACATT GCTGCTGAAG AGGAGGTCTG GGACTATGCT 
CCCTCAGTCC TCGCCCCTGA TGACAGAAGT TATAAAAGTC AATATTTGAA CAATGGCTCT 
CAGCGGATTG GTAGGAAGTA CAAAAAAGTC CGATTTATGG CATACACAGA TGAAACCTTT 
AAGACTCGTG AAGCTATTCA GTATGAATCA GGAATCTTGG GACCTTTACT TTATGGGGAA 
GTTGGAGACA CACTGTTGAT TATATTTAAG AATCAAGCAA GCAGACCATA TAACATCTAC 
CCTCACGGAA TCACTGATGT CCGTCCTTTG TATTCAAGGA GATTACCAAA AGGTGTAAAA 
CATTTGAAGG ATTTTCCAAT TCTGCCAGGA GAAATATTCA AATATAAATG GACAGTGACT 
GTAGAAGATG GGCCAACTAA ATCAGATCCT CGGTGCCTGA CCCGTTATTA CTCTAGTTTC 
ATTAATATGG AGAGAGATCT AGCTTCAGGA CTCATTGGCC CTCTCCTCAT CTGCTACAAA 
GAATCTGTAG ATCAAAGAGG AAACCAGATA ATGTCAGACA AGAGGAATGT CATCCTGTTT 
TCTGTTTTTG ATGAGAACCA AAGCTGGTAC CTCACAGAGA ATATACAACG CTTTCTCCCC 
AATCCAGTTG GAGTGCAGCT TGAGGATCCA GAGTTCCAAG CCTCCAACAT CATGCACAGC 
ATCAATGGCT ATGTTTTTGA TAGTCTGCAG TTGTCAGTTT GTTTGCATGA GGTGGCATAC 
TGGTACATTC TAAGCATTGG AGCACAGACT GACTTCCTTT CTGTCTTCTT CTCTGGATAT 
ACCTTCAAAC ACAAAATGGT CTATGAAGAC ACACTTACCC TATTCCCATT CTCAGGAGAA 
ACTGTCTTCA TGTCGATGGA AAACCCAGGC CTATGGATTC TGGGGTGCCA CAACTCAGAC 
TTTCGGAACA GAGGCATGAC CGCCTTACTG AAGGTTTCTA GTTGTGACAA GAACACTGGT 
GATTATTACG AGGACAGTTA TGAAGATATT TCAACATACT TGCTGAGTAA AAACAATGCC 
ATTGAGCCAA GAAGTTTCTC CCAGAATTCA AGACACCCTA GCCCTAGGCA AAAGCAATTT 
AATGCCACCA CAATTCCCAA AAATGACATA GAGAAGACTG ACCCTTGGTT TGCACACAGA 
ACACCAATGC CTAAAGTACA GAATGTCTCC TCTAGTGATT TGCTGATGCT CTTGCGACAG 
AGTCCTACTC CACATGGGCT ATCCTTATCT GATCTCCAAG AAGCCGAATA TGAGACTTTT 
TCTGATGATC CATCACCTGG AGCAATAGAC ACTAATAACA ACCTTTCTAA AATGACACAC 
CTCAGGCCAC AGCCCCATCA CAGTGGGGAC ATGGTATTTA CCCCTGAGCC AGACCTCCAA 
TTAAGATTAA ATGAGAAACT GGGGACAACT GTAGCAACAG AGTTGAAAAA ATTTGATTTT 
AAAGTTTCTA GTTCATCAAA TAATCTGATT TCAACAATTC CGTCAGACAA TTTGGTAGCA 
GGTAGTGATA ATACAAGTTC CTTAGGACCC CCAAATATAC CAGTTCATTA TGAGAGTCAA 
TTAGATACCA CTCTGTCTGG CAAAAAGTCA TCTCCCCTTA TTGAGTCTGG TGGACCTCTG 
AGTTTGAGTG AAGAAAATAA TGATTCAAAG TTGTTAGAAT CAGGTTTAAT GAATAGCCAA 
GAAAGTTCAT GGGGAAAAAA TGTATGGTCA ACAGAGAGTG GTAGGTTATT TAAAGAGAAA 
AGAGCTCATG GACCTGCTTT GTTGACTAAA GATAATGCCT TATTCAAAGT TAGCATCTCT 
TTGTTAAAGA TAAACAAAAC TTCCAATAAT TCAGCAACTA ATAGAAAGAC TCACATTGAT 
GGCCCATCAT TATTAGTTGA GAATAGTCCA TCAGTCTGGC AAAATATATT AGAAAGTGAT 
ACTGAATTTC AAAAAGTGAC ACCTTTGATT CATGACAGAA TGCTTACAGA CAAAAATACT 
ACAGCTTTGA GGCTAAATCA TATGTCAAAT AAAACTACTT CATCGAAAAA CATGGAAATG 
GTCCAACAAA AAATAGAGGG CCCCATACTA CCAGATGCAG AAAATCCAGA TATGTCATTC 
TTTAAGATGC TATTCTTACC AGAATCAGCA AATTGGATAC AAAGGACTCA TGGAAAGAAC 
TCCCTGAACT CGGGGCAAGG CCCCAGTGCA AAACTATTCA TATCCTTAGG ACCAGAAAAC 
TCTGTGGAAG GTCAGAATTT CTTGTCTGAG AAAAATAAAG TGGTAGTAGG AAAGGGTGAA 
CTTACAAAGG ATATAGGACT CAAAGAGATA GTTTTTCCAA GCAGCAGAAA CCTATTTCTT 
ACTAACTTGG ATAATTTACA TGAAAATAAT ACACACAATC AAGAAAAAAA AATTCAGGAA 
GAAATAGAAA GAAAGGAAAC ATTAATCCAA GATAATATAG TTTTGCCTCA GATACATACA 
GTGACTGGCA CTAAGAATTT CATGAAGAAC CTTTTCTTAC TGAGCACTAG GCAAAATGTA 
GAAGGTTCAT ATGAGGGGGC ATATGCTCCA GTACTTCAAG ATTTTAGGTC ATTAAGTGAT 
TCAACAAATA GAACAAAGAA CCACATGGCT CATTTCTCAG AAAAAGGGGA GGAAGAAAAC 
TTGGAAGGTT TGGGAAATCA AACCAAGCAA ATTGTAGAGA AATATCCACA CACCACAAGG 
ATATCTCCTA ATCCAAACCA GCAAAATTTT GTCACGCAAC GTGGTAAGAG AGCTTTGAAA 
CAATTCAGAC TCCCACTAGA AGAAACAGAA CTTGAAAAAA GGCTAATTGT GGAGGAGACC 
TCAACCCAGT GGTCCAAAAA CATAAAACAT TTGACCCCAA GCACCCTCAC ACAGATAGAC 
TACAATGAGA AGGAGAAAGG AGCCATTACT CAGTCTCCCT TATCAGATTG TCTTACGAGG 
AGTCATAGCA TCACTCAAGC AAATAGATCT CCATTACCCA TTGCAAAGGT ATCATCATTT 
CCATCTATTA GACCTATGGA TCTGACCAGG GTCCTATTCC AAGACAACTT TTCTCGTCTT 
CCAGCACCAC CTTACAGAAA GAAAGATTCT GGGGTCCAAG AAAGCAGTCA TTTCTTACAA 
GGAGTCAAAA AAAATAACCT TTCTTTAGCT ATTCTAACCT TGGAGATGAT TGGTGATCAA 
AGAGAGGTTG GCTCCCTGGT GACAAGTGCT ACAAATTCAG TCACGTACAA GAAAGTTGAG 
AACACTGTTT TCTTGAAACC AGGCTTGCCC GAAACATCTG GCAAAGTTGA ATTGCTTCCA 
AAAGTTCGCA TTTATCAGAA GGACCTTTTC CCTACAGAAA CTAGCAGTGG GTCTCCTGGC 
CATCTGGATC TCATGGAAGG GAGCCTTCTT CAGGAAACAG AGGGAGCAAT TAAGTGGAAG 
GAAGCAAATA GACCTGGAAA AATTCCCTTT CTGAGAGGAG CAACAGAAAG CTCTGCAAAG 
ACTCCTTCCA AGCTATTGGA TCCTCTTGCT TGGGATAACC ACTATGGTAC TCAGATACCA 
AAAGAAGAGT GGAAATCCCA AGAGAAGTCA CCAGAAAACA CAGCTTTTAA GAAAAAGGAT 
ACCATTTTGC CCCTGAACCC TTGTGAAAGC AATCATACAA TAGCAGCAAT AAATGAGGAA 
CAAAATGAGC CCCAAATAGA AGTCACCTGG GCAAAGCAAG GTGGGACTGA AAGGCTGTGC 
TCTCAAAACC CACCAGTCTT GAAACGTCAT CAAAGGGAAA TAACTCTTAA TACTCTTCAG 
TCAGATCAAG AGGAAATTGA CTATGATGAT ACCATCTCAG TTGAAATGAA GAAGGAAGAT 
TTTGACATTT ATGGTGAGGA TGAAAATCAG AGCCCCCGCA GCTTTCAAAA GAAAACACGA 
CACTATTTTA TTGCTGCAGT GGAGCGGCTC TGGGATTATG GGATGAGTAG TTCCCCGCAT 
GTTCTAAGAA ACAGGGCTCA GAGTGGCAGT GTCCCTCAGT TCAAGAAAGT TGTTTTCCAG 
GAATTTACTG ATGGCTCCTT TACTCAGCCC TTATACCGTG GAGAACTAAA TGAACATTTG 
GGACTCCTGG GGCCATATAT AAGAGCAGAA GTTGAAGATA ATATCATGGT AACTTTCAAA 
AATCAGGCCT CTCGTCCCTA TTCCTTCTAT TCTAGCCTTA TTTCTTATGA GGAAGATCAG 
AGGCAAGGAG CAGAACCTAG AAAAAATTTT GTCAAGCCTA ATGAAACCAA AACTTACTTT 
TGGAAAGTGC AACATCATAT GGCGCCCACT AAAGATGAGT TTGACTGCAA AGCCTGGGCT 
TATTTCTCTG ATGTTGACCT GGAAAAAGAT GTGCACTCAG GCCTGATTGG ACCCCTTCTG 
GTCTGCCACA CTAACACACT GAACCCTGCT CATGGGAGAC AAGTGACAGT ACAGGAATTT 
GCTCTGTTTT TCACTATCTT TGATGAGACC AAAAGCTGGT ACTTCACTGA AAACACAGAA 
AGAAACTGCA GGGCTCCCTG CAATATCCAG ATGGAAGATC CCACTTTTAA AGAGAATTAT 
CGCTTCCATG CAATCAATGG CTACATAATG GATACACTAC CTGGCTTAGT AATGGCTCAG 
GATCAAAGGA TTCGATGGTA TCTGCTCAGC ATGGGCAGCA ATGAAAACAT CCATTCTATT 
CATTTTAGTG GACATGTGTT CACTGTACGA AAAAAAGAGG AGTATAAAAT GGCAGTGTAC 
AATCTCTATC CAGGTGTTTT TGAGACAGTT GAAATGTTAC CATCCAAAGC TGGAATTTGG 
CGGGTGGAAT GCCTTATTGG CGAGCACCTA CATGCTGGGA TGAGCACACT TTTTCTGGTG 
TACAGCAACA AGTGTCAGAC TCCCCTGGGA ATGGCTTCTG GACGCATTAG AGATTTTCAG 
ATTACAGCTT CAGGACAATA TGGACAGTGG GCCCCAAAGC TGGCCAGACT TCATTATTCT 
GGATCAATCA ATGCCTGGAG CACCAAGGAG CCCTTTTCTT GGATCAAGGT GGATCTGTTG 
GCACCAATGA TTATTCACGG CATCAAGACC CAGGGTGCCC GTCAGAAGTT CTCCAGCCTC 
TACATCTCTC AGTTTATCAT CATGTATAGT CTTGATGGGA AAAAGTGGCA GACTTATCGA 
GGAAATTCCA CTGGAACCTT AATGGTATGT AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014984060.1
CDS	466..6801
Translation	MQSDLGELPVDTRFPPRVPRSFPFNTSVMYKKTVFVEFTDHLFNIAKPRPPWMGLLGPTIQAEVYDTVVITLKNMASHPVSLHAVGVSYWKASEGAEYDDQTSQREKEDDKVFPGGSHTYVWQVLKENGPMASDPLCLTYSYLSHVDLVKDLNSGLIGALLVCREGSLAKEKTQTLHKFVLLFAVFDEGKSWHSETKNSLMQDRDDASARAWPKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTLLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKNNEEAEDYDDDLADSEMDVVRFDDDNSPSFIQIRSVAKKHPKTWVHYIAAEEEVWDYAPSVLAPDDRSYKSQYLNNGSQRIGRKYKKVRFMAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVRPLYSRRLPKGVKHLKDFPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINMERDLASGLIGPLLICYKESVDQRGNQIMSDKRNVILFSVFDENQSWYLTENIQRFLPNPVGVQLEDPEFQASNIMHSINGYVFDSLQLSVCLHEVAYWYILSIGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISTYLLSKNNAIEPRSFSQNSRHPSPRQKQFNATTIPKNDIEKTDPWFAHRTPMPKVQNVSSSDLLMLLRQSPTPHGLSLSDLQEAEYETFSDDPSPGAIDTNNNLSKMTHLRPQPHHSGDMVFTPEPDLQLRLNEKLGTTVATELKKFDFKVSSSSNNLISTIPSDNLVAGSDNTSSLGPPNIPVHYESQLDTTLSGKKSSPLIESGGPLSLSEENNDSKLLESGLMNSQESSWGKNVWSTESGRLFKEKRAHGPALLTKDNALFKVSISLLKINKTSNNSATNRKTHIDGPSLLVENSPSVWQNILESDTEFQKVTPLIHDRMLTDKNTTALRLNHMSNKTTSSKNMEMVQQKIEGPILPDAENPDMSFFKMLFLPESANWIQRTHGKNSLNSGQGPSAKLFISLGPENSVEGQNFLSEKNKVVVGKGELTKDIGLKEIVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIERKETLIQDNIVLPQIHTVTGTKNFMKNLFLLSTRQNVEGSYEGAYAPVLQDFRSLSDSTNRTKNHMAHFSEKGEEENLEGLGNQTKQIVEKYPHTTRISPNPNQQNFVTQRGKRALKQFRLPLEETELEKRLIVEETSTQWSKNIKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSITQANRSPLPIAKVSSFPSIRPMDLTRVLFQDNFSRLPAPPYRKKDSGVQESSHFLQGVKKNNLSLAILTLEMIGDQREVGSLVTSATNSVTYKKVENTVFLKPGLPETSGKVELLPKVRIYQKDLFPTETSSGSPGHLDLMEGSLLQETEGAIKWKEANRPGKIPFLRGATESSAKTPSKLLDPLAWDNHYGTQIPKEEWKSQEKSPENTAFKKKDTILPLNPCESNHTIAAINEEQNEPQIEVTWAKQGGTERLCSQNPPVLKRHQREITLNTLQSDQEEIDYDDTISVEMKKEDFDIYGEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSGSVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFDETKSWYFTENTERNCRAPCNIQMEDPTFKENYRFHAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMAVYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVCN

Target ORF information:

RefSeq Version	XM_015128574.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta coagulation factor VIII, procoagulant component (F8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015128574.1

ORF Insert Sequence:

ATGCAAAGTG ATCTCGGTGA GCTACCTGTG GACACAAGAT TTCCTCCTAG AGTGCCAAGA 
TCTTTTCCAT TCAACACCTC AGTCATGTAC AAAAAGACTG TGTTTGTAGA ATTCACGGAT 
CACCTTTTCA ACATCGCTAA GCCAAGGCCA CCTTGGATGG GTCTGCTGGG TCCTACTATC 
CAGGCTGAGG TTTATGACAC AGTGGTCATT ACACTTAAGA ACATGGCTTC CCATCCTGTC 
AGTCTTCATG CTGTTGGTGT TTCCTACTGG AAAGCTTCTG AGGGAGCTGA ATATGATGAT 
CAGACCAGTC AAAGGGAGAA AGAAGATGAT AAAGTCTTTC CTGGTGGAAG CCATACATAT 
GTCTGGCAGG TCCTGAAAGA GAATGGTCCA ATGGCCTCTG ACCCGCTGTG CCTTACCTAC 
TCGTATCTTT CTCATGTGGA CCTGGTAAAA GACTTGAATT CGGGCCTCAT TGGAGCCCTA 
CTAGTATGTA GAGAAGGGAG TCTGGCCAAG GAAAAGACAC AGACCTTGCA CAAATTTGTA 
CTACTTTTTG CTGTATTTGA TGAAGGGAAA AGTTGGCACT CAGAAACAAA GAACTCCTTG 
ATGCAGGATA GGGATGATGC ATCTGCTCGG GCCTGGCCTA AAATGCACAC AGTCAATGGT 
TATGTAAACA GGTCTCTACC AGGTCTGATT GGATGCCACA GGAAGTCAGT CTATTGGCAT 
GTGATTGGAA TGGGCACCAC TCCTGAAGTG CACTCAATAT TCCTCGAAGG TCACACATTT 
CTTGTGAGGA ACCATCGCCA GGCCTCCTTG GAAATCTCAC CAATAACTTT CCTTACTGCT 
CAAACACTCC TGATGGACCT TGGACAGTTT CTACTGTTTT GTCATATCTC TTCCCACCAA 
CATGATGGCA TGGAAGCTTA TGTCAAAGTA GACAGTTGCC CAGAGGAACC CCAACTACGA 
ATGAAAAATA ATGAAGAAGC GGAAGACTAT GATGATGATC TTGCTGATTC TGAAATGGAT 
GTGGTCAGGT TTGATGATGA CAACTCTCCT TCCTTTATCC AAATTCGCTC AGTTGCCAAG 
AAGCATCCTA AAACTTGGGT ACATTACATT GCTGCTGAAG AGGAGGTCTG GGACTATGCT 
CCCTCAGTCC TCGCCCCTGA TGACAGAAGT TATAAAAGTC AATATTTGAA CAATGGCTCT 
CAGCGGATTG GTAGGAAGTA CAAAAAAGTC CGATTTATGG CATACACAGA TGAAACCTTT 
AAGACTCGTG AAGCTATTCA GTATGAATCA GGAATCTTGG GACCTTTACT TTATGGGGAA 
GTTGGAGACA CACTGTTGAT TATATTTAAG AATCAAGCAA GCAGACCATA TAACATCTAC 
CCTCACGGAA TCACTGATGT CCGTCCTTTG TATTCAAGGA GATTACCAAA AGGTGTAAAA 
CATTTGAAGG ATTTTCCAAT TCTGCCAGGA GAAATATTCA AATATAAATG GACAGTGACT 
GTAGAAGATG GGCCAACTAA ATCAGATCCT CGGTGCCTGA CCCGTTATTA CTCTAGTTTC 
ATTAATATGG AGAGAGATCT AGCTTCAGGA CTCATTGGCC CTCTCCTCAT CTGCTACAAA 
GAATCTGTAG ATCAAAGAGG AAACCAGATA ATGTCAGACA AGAGGAATGT CATCCTGTTT 
TCTGTTTTTG ATGAGAACCA AAGCTGGTAC CTCACAGAGA ATATACAACG CTTTCTCCCC 
AATCCAGTTG GAGTGCAGCT TGAGGATCCA GAGTTCCAAG CCTCCAACAT CATGCACAGC 
ATCAATGGCT ATGTTTTTGA TAGTCTGCAG TTGTCAGTTT GTTTGCATGA GGTGGCATAC 
TGGTACATTC TAAGCATTGG AGCACAGACT GACTTCCTTT CTGTCTTCTT CTCTGGATAT 
ACCTTCAAAC ACAAAATGGT CTATGAAGAC ACACTTACCC TATTCCCATT CTCAGGAGAA 
ACTGTCTTCA TGTCGATGGA AAACCCAGGC CTATGGATTC TGGGGTGCCA CAACTCAGAC 
TTTCGGAACA GAGGCATGAC CGCCTTACTG AAGGTTTCTA GTTGTGACAA GAACACTGGT 
GATTATTACG AGGACAGTTA TGAAGATATT TCAACATACT TGCTGAGTAA AAACAATGCC 
ATTGAGCCAA GAAGTTTCTC CCAGAATTCA AGACACCCTA GCCCTAGGCA AAAGCAATTT 
AATGCCACCA CAATTCCCAA AAATGACATA GAGAAGACTG ACCCTTGGTT TGCACACAGA 
ACACCAATGC CTAAAGTACA GAATGTCTCC TCTAGTGATT TGCTGATGCT CTTGCGACAG 
AGTCCTACTC CACATGGGCT ATCCTTATCT GATCTCCAAG AAGCCGAATA TGAGACTTTT 
TCTGATGATC CATCACCTGG AGCAATAGAC ACTAATAACA ACCTTTCTAA AATGACACAC 
CTCAGGCCAC AGCCCCATCA CAGTGGGGAC ATGGTATTTA CCCCTGAGCC AGACCTCCAA 
TTAAGATTAA ATGAGAAACT GGGGACAACT GTAGCAACAG AGTTGAAAAA ATTTGATTTT 
AAAGTTTCTA GTTCATCAAA TAATCTGATT TCAACAATTC CGTCAGACAA TTTGGTAGCA 
GGTAGTGATA ATACAAGTTC CTTAGGACCC CCAAATATAC CAGTTCATTA TGAGAGTCAA 
TTAGATACCA CTCTGTCTGG CAAAAAGTCA TCTCCCCTTA TTGAGTCTGG TGGACCTCTG 
AGTTTGAGTG AAGAAAATAA TGATTCAAAG TTGTTAGAAT CAGGTTTAAT GAATAGCCAA 
GAAAGTTCAT GGGGAAAAAA TGTATGGTCA ACAGAGAGTG GTAGGTTATT TAAAGAGAAA 
AGAGCTCATG GACCTGCTTT GTTGACTAAA GATAATGCCT TATTCAAAGT TAGCATCTCT 
TTGTTAAAGA TAAACAAAAC TTCCAATAAT TCAGCAACTA ATAGAAAGAC TCACATTGAT 
GGCCCATCAT TATTAGTTGA GAATAGTCCA TCAGTCTGGC AAAATATATT AGAAAGTGAT 
ACTGAATTTC AAAAAGTGAC ACCTTTGATT CATGACAGAA TGCTTACAGA CAAAAATACT 
ACAGCTTTGA GGCTAAATCA TATGTCAAAT AAAACTACTT CATCGAAAAA CATGGAAATG 
GTCCAACAAA AAATAGAGGG CCCCATACTA CCAGATGCAG AAAATCCAGA TATGTCATTC 
TTTAAGATGC TATTCTTACC AGAATCAGCA AATTGGATAC AAAGGACTCA TGGAAAGAAC 
TCCCTGAACT CGGGGCAAGG CCCCAGTGCA AAACTATTCA TATCCTTAGG ACCAGAAAAC 
TCTGTGGAAG GTCAGAATTT CTTGTCTGAG AAAAATAAAG TGGTAGTAGG AAAGGGTGAA 
CTTACAAAGG ATATAGGACT CAAAGAGATA GTTTTTCCAA GCAGCAGAAA CCTATTTCTT 
ACTAACTTGG ATAATTTACA TGAAAATAAT ACACACAATC AAGAAAAAAA AATTCAGGAA 
GAAATAGAAA GAAAGGAAAC ATTAATCCAA GATAATATAG TTTTGCCTCA GATACATACA 
GTGACTGGCA CTAAGAATTT CATGAAGAAC CTTTTCTTAC TGAGCACTAG GCAAAATGTA 
GAAGGTTCAT ATGAGGGGGC ATATGCTCCA GTACTTCAAG ATTTTAGGTC ATTAAGTGAT 
TCAACAAATA GAACAAAGAA CCACATGGCT CATTTCTCAG AAAAAGGGGA GGAAGAAAAC 
TTGGAAGGTT TGGGAAATCA AACCAAGCAA ATTGTAGAGA AATATCCACA CACCACAAGG 
ATATCTCCTA ATCCAAACCA GCAAAATTTT GTCACGCAAC GTGGTAAGAG AGCTTTGAAA 
CAATTCAGAC TCCCACTAGA AGAAACAGAA CTTGAAAAAA GGCTAATTGT GGAGGAGACC 
TCAACCCAGT GGTCCAAAAA CATAAAACAT TTGACCCCAA GCACCCTCAC ACAGATAGAC 
TACAATGAGA AGGAGAAAGG AGCCATTACT CAGTCTCCCT TATCAGATTG TCTTACGAGG 
AGTCATAGCA TCACTCAAGC AAATAGATCT CCATTACCCA TTGCAAAGGT ATCATCATTT 
CCATCTATTA GACCTATGGA TCTGACCAGG GTCCTATTCC AAGACAACTT TTCTCGTCTT 
CCAGCACCAC CTTACAGAAA GAAAGATTCT GGGGTCCAAG AAAGCAGTCA TTTCTTACAA 
GGAGTCAAAA AAAATAACCT TTCTTTAGCT ATTCTAACCT TGGAGATGAT TGGTGATCAA 
AGAGAGGTTG GCTCCCTGGT GACAAGTGCT ACAAATTCAG TCACGTACAA GAAAGTTGAG 
AACACTGTTT TCTTGAAACC AGGCTTGCCC GAAACATCTG GCAAAGTTGA ATTGCTTCCA 
AAAGTTCGCA TTTATCAGAA GGACCTTTTC CCTACAGAAA CTAGCAGTGG GTCTCCTGGC 
CATCTGGATC TCATGGAAGG GAGCCTTCTT CAGGAAACAG AGGGAGCAAT TAAGTGGAAG 
GAAGCAAATA GACCTGGAAA AATTCCCTTT CTGAGAGGAG CAACAGAAAG CTCTGCAAAG 
ACTCCTTCCA AGCTATTGGA TCCTCTTGCT TGGGATAACC ACTATGGTAC TCAGATACCA 
AAAGAAGAGT GGAAATCCCA AGAGAAGTCA CCAGAAAACA CAGCTTTTAA GAAAAAGGAT 
ACCATTTTGC CCCTGAACCC TTGTGAAAGC AATCATACAA TAGCAGCAAT AAATGAGGAA 
CAAAATGAGC CCCAAATAGA AGTCACCTGG GCAAAGCAAG GTGGGACTGA AAGGCTGTGC 
TCTCAAAACC CACCAGTCTT GAAACGTCAT CAAAGGGAAA TAACTCTTAA TACTCTTCAG 
TCAGATCAAG AGGAAATTGA CTATGATGAT ACCATCTCAG TTGAAATGAA GAAGGAAGAT 
TTTGACATTT ATGGTGAGGA TGAAAATCAG AGCCCCCGCA GCTTTCAAAA GAAAACACGA 
CACTATTTTA TTGCTGCAGT GGAGCGGCTC TGGGATTATG GGATGAGTAG TTCCCCGCAT 
GTTCTAAGAA ACAGGGCTCA GAGTGGCAGT GTCCCTCAGT TCAAGAAAGT TGTTTTCCAG 
GAATTTACTG ATGGCTCCTT TACTCAGCCC TTATACCGTG GAGAACTAAA TGAACATTTG 
GGACTCCTGG GGCCATATAT AAGAGCAGAA GTTGAAGATA ATATCATGGT AACTTTCAAA 
AATCAGGCCT CTCGTCCCTA TTCCTTCTAT TCTAGCCTTA TTTCTTATGA GGAAGATCAG 
AGGCAAGGAG CAGAACCTAG AAAAAATTTT GTCAAGCCTA ATGAAACCAA AACTTACTTT 
TGGAAAGTGC AACATCATAT GGCGCCCACT AAAGATGAGT TTGACTGCAA AGCCTGGGCT 
TATTTCTCTG ATGTTGACCT GGAAAAAGAT GTGCACTCAG GCCTGATTGG ACCCCTTCTG 
GTCTGCCACA CTAACACACT GAACCCTGCT CATGGGAGAC AAGTGACAGT ACAGGAATTT 
GCTCTGTTTT TCACTATCTT TGATGAGACC AAAAGCTGGT ACTTCACTGA AAACACAGAA 
AGAAACTGCA GGGCTCCCTG CAATATCCAG ATGGAAGATC CCACTTTTAA AGAGAATTAT 
CGCTTCCATG CAATCAATGG CTACATAATG GATACACTAC CTGGCTTAGT AATGGCTCAG 
GATCAAAGGA TTCGATGGTA TCTGCTCAGC ATGGGCAGCA ATGAAAACAT CCATTCTATT 
CATTTTAGTG GACATGTGTT CACTGTACGA AAAAAAGAGG AGTATAAAAT GGCAGTGTAC 
AATCTCTATC CAGGTGTTTT TGAGACAGTT GAAATGTTAC CATCCAAAGC TGGAATTTGG 
CGGGTGGAAT GCCTTATTGG CGAGCACCTA CATGCTGGGA TGAGCACACT TTTTCTGGTG 
TACAGCAACA AGTGTCAGAC TCCCCTGGGA ATGGCTTCTG GACGCATTAG AGATTTTCAG 
ATTACAGCTT CAGGACAATA TGGACAGTGG GCCCCAAAGC TGGCCAGACT TCATTATTCT 
GGATCAATCA ATGCCTGGAG CACCAAGGAG CCCTTTTCTT GGATCAAGGT GGATCTGTTG 
GCACCAATGA TTATTCACGG CATCAAGACC CAGGGTGCCC GTCAGAAGTT CTCCAGCCTC 
TACATCTCTC AGTTTATCAT CATGTATAGT CTTGATGGGA AAAAGTGGCA GACTTATCGA 
GGAAATTCCA CTGGAACCTT AATGGTATGT AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_015128573.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014984059.2 Macaca mulatta coagulation factor VIII (F8), transcript variant X1, mRNA.

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CloneID	OMb94357
Clone ID Related Accession (Same CDS sequence)	XM_015128573.2
Accession Version	XM_015128573.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	coagulation factor VIII isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015128573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAATAG AGCTCTCCAC CTACTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC 
GGTGAGCTAC CTGTGGACAC AAGATTTCCT CCTAGAGTGC CAAGATCTTT TCCATTCAAC 
ACCTCAGTCA TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC 
GCTAAGCCAA GGCCACCTTG GATGGGTCTG CTGGGTCCTA CTATCCAGGC TGAGGTTTAT 
GACACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT 
GGTGTTTCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG 
GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG 
AAAGAGAATG GTCCAATGGC CTCTGACCCG CTGTGCCTTA CCTACTCGTA TCTTTCTCAT 
GTGGACCTGG TAAAAGACTT GAATTCGGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA 
GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTGTACTACT TTTTGCTGTA 
TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT 
GATGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT 
CTACCAGGTC TGATTGGATG CCACAGGAAG TCAGTCTATT GGCATGTGAT TGGAATGGGC 
ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT 
CGCCAGGCCT CCTTGGAAAT CTCACCAATA ACTTTCCTTA CTGCTCAAAC ACTCCTGATG 
GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA 
GCTTATGTCA AAGTAGACAG TTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA 
GAAGCGGAAG ACTATGATGA TGATCTTGCT GATTCTGAAA TGGATGTGGT CAGGTTTGAT 
GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT 
TGGGTACATT ACATTGCTGC TGAAGAGGAG GTCTGGGACT ATGCTCCCTC AGTCCTCGCC 
CCTGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCTCTCAGCG GATTGGTAGG 
AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT 
ATTCAGTATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG 
TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT 
GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT 
CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA 
ACTAAATCAG ATCCTCGGTG CCTGACCCGT TATTACTCTA GTTTCATTAA TATGGAGAGA 
GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA 
AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT TTTTGATGAG 
AACCAAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGTTGGAGTG 
CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT 
TTTGATAGTC TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC 
ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA 
ATGGTCTATG AAGACACACT TACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAAC ATACTTGCTG AGTAAAAACA ATGCCATTGA GCCAAGAAGT 
TTCTCCCAGA ATTCAAGACA CCCTAGCCCT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCCAAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC AATGCCTAAA 
GTACAGAATG TCTCCTCTAG TGATTTGCTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC GAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACACTAA TAACAACCTT TCTAAAATGA CACACCTCAG GCCACAGCCC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGCCAGACC TCCAATTAAG ATTAAATGAG 
AAACTGGGGA CAACTGTAGC AACAGAGTTG AAAAAATTTG ATTTTAAAGT TTCTAGTTCA 
TCAAATAATC TGATTTCAAC AATTCCGTCA GACAATTTGG TAGCAGGTAG TGATAATACA 
AGTTCCTTAG GACCCCCAAA TATACCAGTT CATTATGAGA GTCAATTAGA TACCACTCTG 
TCTGGCAAAA AGTCATCTCC CCTTATTGAG TCTGGTGGAC CTCTGAGTTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT GGTCAACAGA GAGTGGTAGG TTATTTAAAG AGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGATAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
GTTGAGAATA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGATACTGA ATTTCAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ACAGACAAAA ATACTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCG AAAAACATGG AAATGGTCCA ACAAAAAATA 
GAGGGCCCCA TACTACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTACCAGAAT CAGCAAATTG GATACAAAGG ACTCATGGAA AGAACTCCCT GAACTCGGGG 
CAAGGCCCCA GTGCAAAACT ATTCATATCC TTAGGACCAG AAAACTCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA TAAAGTGGTA GTAGGAAAGG GTGAACTTAC AAAGGATATA 
GGACTCAAAG AGATAGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGAAAG 
GAAACATTAA TCCAAGATAA TATAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA GTGATTCAAC AAATAGAACA 
AAGAACCACA TGGCTCATTT CTCAGAAAAA GGGGAGGAAG AAAACTTGGA AGGTTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT CCACACACCA CAAGGATATC TCCTAATCCA 
AACCAGCAAA ATTTTGTCAC GCAACGTGGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGCTA ATTGTGGAGG AGACCTCAAC CCAGTGGTCC 
AAAAACATAA AACATTTGAC CCCAAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGAGCCA TTACTCAGTC TCCCTTATCA GATTGTCTTA CGAGGAGTCA TAGCATCACT 
CAAGCAAATA GATCTCCATT ACCCATTGCA AAGGTATCAT CATTTCCATC TATTAGACCT 
ATGGATCTGA CCAGGGTCCT ATTCCAAGAC AACTTTTCTC GTCTTCCAGC ACCACCTTAC 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGT CAAAAAAAAT 
AACCTTTCTT TAGCTATTCT AACCTTGGAG ATGATTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGTGACAA GTGCTACAAA TTCAGTCACG TACAAGAAAG TTGAGAACAC TGTTTTCTTG 
AAACCAGGCT TGCCCGAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCGCATTTAT 
CAGAAGGACC TTTTCCCTAC AGAAACTAGC AGTGGGTCTC CTGGCCATCT GGATCTCATG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCAATTAAGT GGAAGGAAGC AAATAGACCT 
GGAAAAATTC CCTTTCTGAG AGGAGCAACA GAAAGCTCTG CAAAGACTCC TTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAACACAGCT TTTAAGAAAA AGGATACCAT TTTGCCCCTG 
AACCCTTGTG AAAGCAATCA TACAATAGCA GCAATAAATG AGGAACAAAA TGAGCCCCAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTGGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GTCTTGAAAC GTCATCAAAG GGAAATAACT CTTAATACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGGT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGC GGCTCTGGGA TTATGGGATG AGTAGTTCCC CGCATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGAAAAA ATTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC 
ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACT 
ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA CAGAAAGAAA CTGCAGGGCT 
CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC 
AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA 
TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT TAGTGGACAT 
GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAG TGTACAATCT CTATCCAGGT 
GTTTTTGAGA CAGTTGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT 
ATTGGCGAGC ACCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAACAAGTGT 
CAGACTCCCC TGGGAATGGC TTCTGGACGC ATTAGAGATT TTCAGATTAC AGCTTCAGGA 
CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCTGGATC AATCAATGCC 
TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT 
CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT 
ATCATCATGT ATAGTCTTGA TGGGAAAAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA 
ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC 
CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT 
CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCGTT GGGAATGGAG 
AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTCACCAA TATGTTTGCC 
ACCTGGTCTC CTTCAAAAGC TCGACTTAAC CTCCAAGGAA GGAGTAATGC CTGGAGACCT 
CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA 
GGAATAACTA CTCAGGGAGT AAAATCTCTA CTTACCAACA TGTATGTGAA GGAGTTCCTC 
ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTCT TTCAGAATGG CAAAGTAAAG 
GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CTCACCGTTA 
CTGACTCGCT ACCTTCGAAT TCATCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATA 
GAGGTTCTGG GCTGCGAGGC ACAGGAGCTC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014984059.2
CDS	458..7513
Translation	MQIELSTYFFLCLLRFCFSATRRYYLGAVELSWDYMQSDLGELPVDTRFPPRVPRSFPFNTSVMYKKTVFVEFTDHLFNIAKPRPPWMGLLGPTIQAEVYDTVVITLKNMASHPVSLHAVGVSYWKASEGAEYDDQTSQREKEDDKVFPGGSHTYVWQVLKENGPMASDPLCLTYSYLSHVDLVKDLNSGLIGALLVCREGSLAKEKTQTLHKFVLLFAVFDEGKSWHSETKNSLMQDRDDASARAWPKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTLLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKNNEEAEDYDDDLADSEMDVVRFDDDNSPSFIQIRSVAKKHPKTWVHYIAAEEEVWDYAPSVLAPDDRSYKSQYLNNGSQRIGRKYKKVRFMAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVRPLYSRRLPKGVKHLKDFPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINMERDLASGLIGPLLICYKESVDQRGNQIMSDKRNVILFSVFDENQSWYLTENIQRFLPNPVGVQLEDPEFQASNIMHSINGYVFDSLQLSVCLHEVAYWYILSIGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISTYLLSKNNAIEPRSFSQNSRHPSPRQKQFNATTIPKNDIEKTDPWFAHRTPMPKVQNVSSSDLLMLLRQSPTPHGLSLSDLQEAEYETFSDDPSPGAIDTNNNLSKMTHLRPQPHHSGDMVFTPEPDLQLRLNEKLGTTVATELKKFDFKVSSSSNNLISTIPSDNLVAGSDNTSSLGPPNIPVHYESQLDTTLSGKKSSPLIESGGPLSLSEENNDSKLLESGLMNSQESSWGKNVWSTESGRLFKEKRAHGPALLTKDNALFKVSISLLKINKTSNNSATNRKTHIDGPSLLVENSPSVWQNILESDTEFQKVTPLIHDRMLTDKNTTALRLNHMSNKTTSSKNMEMVQQKIEGPILPDAENPDMSFFKMLFLPESANWIQRTHGKNSLNSGQGPSAKLFISLGPENSVEGQNFLSEKNKVVVGKGELTKDIGLKEIVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIERKETLIQDNIVLPQIHTVTGTKNFMKNLFLLSTRQNVEGSYEGAYAPVLQDFRSLSDSTNRTKNHMAHFSEKGEEENLEGLGNQTKQIVEKYPHTTRISPNPNQQNFVTQRGKRALKQFRLPLEETELEKRLIVEETSTQWSKNIKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSITQANRSPLPIAKVSSFPSIRPMDLTRVLFQDNFSRLPAPPYRKKDSGVQESSHFLQGVKKNNLSLAILTLEMIGDQREVGSLVTSATNSVTYKKVENTVFLKPGLPETSGKVELLPKVRIYQKDLFPTETSSGSPGHLDLMEGSLLQETEGAIKWKEANRPGKIPFLRGATESSAKTPSKLLDPLAWDNHYGTQIPKEEWKSQEKSPENTAFKKKDTILPLNPCESNHTIAAINEEQNEPQIEVTWAKQGGTERLCSQNPPVLKRHQREITLNTLQSDQEEIDYDDTISVEMKKEDFDIYGEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSGSVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFDETKSWYFTENTERNCRAPCNIQMEDPTFKENYRFHAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMAVYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLNLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTNMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDSPLLTRYLRIHPQSWVHQIALRIEVLGCEAQELY

Target ORF information:

RefSeq Version	XM_015128573.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta coagulation factor VIII (F8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015128573.2

ORF Insert Sequence:

ATGCAAATAG AGCTCTCCAC CTACTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC 
GGTGAGCTAC CTGTGGACAC AAGATTTCCT CCTAGAGTGC CAAGATCTTT TCCATTCAAC 
ACCTCAGTCA TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC 
GCTAAGCCAA GGCCACCTTG GATGGGTCTG CTGGGTCCTA CTATCCAGGC TGAGGTTTAT 
GACACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT 
GGTGTTTCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG 
GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG 
AAAGAGAATG GTCCAATGGC CTCTGACCCG CTGTGCCTTA CCTACTCGTA TCTTTCTCAT 
GTGGACCTGG TAAAAGACTT GAATTCGGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA 
GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTGTACTACT TTTTGCTGTA 
TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT 
GATGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT 
CTACCAGGTC TGATTGGATG CCACAGGAAG TCAGTCTATT GGCATGTGAT TGGAATGGGC 
ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT 
CGCCAGGCCT CCTTGGAAAT CTCACCAATA ACTTTCCTTA CTGCTCAAAC ACTCCTGATG 
GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA 
GCTTATGTCA AAGTAGACAG TTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA 
GAAGCGGAAG ACTATGATGA TGATCTTGCT GATTCTGAAA TGGATGTGGT CAGGTTTGAT 
GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT 
TGGGTACATT ACATTGCTGC TGAAGAGGAG GTCTGGGACT ATGCTCCCTC AGTCCTCGCC 
CCTGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCTCTCAGCG GATTGGTAGG 
AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT 
ATTCAGTATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG 
TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT 
GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT 
CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA 
ACTAAATCAG ATCCTCGGTG CCTGACCCGT TATTACTCTA GTTTCATTAA TATGGAGAGA 
GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA 
AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT TTTTGATGAG 
AACCAAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGTTGGAGTG 
CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT 
TTTGATAGTC TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC 
ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA 
ATGGTCTATG AAGACACACT TACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAAC ATACTTGCTG AGTAAAAACA ATGCCATTGA GCCAAGAAGT 
TTCTCCCAGA ATTCAAGACA CCCTAGCCCT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCCAAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC AATGCCTAAA 
GTACAGAATG TCTCCTCTAG TGATTTGCTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC GAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACACTAA TAACAACCTT TCTAAAATGA CACACCTCAG GCCACAGCCC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGCCAGACC TCCAATTAAG ATTAAATGAG 
AAACTGGGGA CAACTGTAGC AACAGAGTTG AAAAAATTTG ATTTTAAAGT TTCTAGTTCA 
TCAAATAATC TGATTTCAAC AATTCCGTCA GACAATTTGG TAGCAGGTAG TGATAATACA 
AGTTCCTTAG GACCCCCAAA TATACCAGTT CATTATGAGA GTCAATTAGA TACCACTCTG 
TCTGGCAAAA AGTCATCTCC CCTTATTGAG TCTGGTGGAC CTCTGAGTTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT GGTCAACAGA GAGTGGTAGG TTATTTAAAG AGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGATAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
GTTGAGAATA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGATACTGA ATTTCAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ACAGACAAAA ATACTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCG AAAAACATGG AAATGGTCCA ACAAAAAATA 
GAGGGCCCCA TACTACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTACCAGAAT CAGCAAATTG GATACAAAGG ACTCATGGAA AGAACTCCCT GAACTCGGGG 
CAAGGCCCCA GTGCAAAACT ATTCATATCC TTAGGACCAG AAAACTCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA TAAAGTGGTA GTAGGAAAGG GTGAACTTAC AAAGGATATA 
GGACTCAAAG AGATAGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGAAAG 
GAAACATTAA TCCAAGATAA TATAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA GTGATTCAAC AAATAGAACA 
AAGAACCACA TGGCTCATTT CTCAGAAAAA GGGGAGGAAG AAAACTTGGA AGGTTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT CCACACACCA CAAGGATATC TCCTAATCCA 
AACCAGCAAA ATTTTGTCAC GCAACGTGGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGCTA ATTGTGGAGG AGACCTCAAC CCAGTGGTCC 
AAAAACATAA AACATTTGAC CCCAAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGAGCCA TTACTCAGTC TCCCTTATCA GATTGTCTTA CGAGGAGTCA TAGCATCACT 
CAAGCAAATA GATCTCCATT ACCCATTGCA AAGGTATCAT CATTTCCATC TATTAGACCT 
ATGGATCTGA CCAGGGTCCT ATTCCAAGAC AACTTTTCTC GTCTTCCAGC ACCACCTTAC 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGT CAAAAAAAAT 
AACCTTTCTT TAGCTATTCT AACCTTGGAG ATGATTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGTGACAA GTGCTACAAA TTCAGTCACG TACAAGAAAG TTGAGAACAC TGTTTTCTTG 
AAACCAGGCT TGCCCGAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCGCATTTAT 
CAGAAGGACC TTTTCCCTAC AGAAACTAGC AGTGGGTCTC CTGGCCATCT GGATCTCATG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCAATTAAGT GGAAGGAAGC AAATAGACCT 
GGAAAAATTC CCTTTCTGAG AGGAGCAACA GAAAGCTCTG CAAAGACTCC TTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAACACAGCT TTTAAGAAAA AGGATACCAT TTTGCCCCTG 
AACCCTTGTG AAAGCAATCA TACAATAGCA GCAATAAATG AGGAACAAAA TGAGCCCCAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTGGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GTCTTGAAAC GTCATCAAAG GGAAATAACT CTTAATACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGGT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGC GGCTCTGGGA TTATGGGATG AGTAGTTCCC CGCATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGAAAAA ATTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC 
ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACT 
ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA CAGAAAGAAA CTGCAGGGCT 
CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC 
AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA 
TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT TAGTGGACAT 
GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAG TGTACAATCT CTATCCAGGT 
GTTTTTGAGA CAGTTGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT 
ATTGGCGAGC ACCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAACAAGTGT 
CAGACTCCCC TGGGAATGGC TTCTGGACGC ATTAGAGATT TTCAGATTAC AGCTTCAGGA 
CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCTGGATC AATCAATGCC 
TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT 
CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT 
ATCATCATGT ATAGTCTTGA TGGGAAAAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA 
ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC 
CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT 
CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCGTT GGGAATGGAG 
AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTCACCAA TATGTTTGCC 
ACCTGGTCTC CTTCAAAAGC TCGACTTAAC CTCCAAGGAA GGAGTAATGC CTGGAGACCT 
CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA 
GGAATAACTA CTCAGGGAGT AAAATCTCTA CTTACCAACA TGTATGTGAA GGAGTTCCTC 
ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTCT TTCAGAATGG CAAAGTAAAG 
GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CTCACCGTTA 
CTGACTCGCT ACCTTCGAAT TCATCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATA 
GAGGTTCTGG GCTGCGAGGC ACAGGAGCTC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_015128574.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014984060.2 Macaca mulatta coagulation factor VIII (F8), transcript variant X2, mRNA.

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CloneID	OMb94358
Clone ID Related Accession (Same CDS sequence)	XM_015128574.2
Accession Version	XM_015128574.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	coagulation factor VIII isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015128574.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGTG ATCTCGGTGA GCTACCTGTG GACACAAGAT TTCCTCCTAG AGTGCCAAGA 
TCTTTTCCAT TCAACACCTC AGTCATGTAC AAAAAGACTG TGTTTGTAGA ATTCACGGAT 
CACCTTTTCA ACATCGCTAA GCCAAGGCCA CCTTGGATGG GTCTGCTGGG TCCTACTATC 
CAGGCTGAGG TTTATGACAC AGTGGTCATT ACACTTAAGA ACATGGCTTC CCATCCTGTC 
AGTCTTCATG CTGTTGGTGT TTCCTACTGG AAAGCTTCTG AGGGAGCTGA ATATGATGAT 
CAGACCAGTC AAAGGGAGAA AGAAGATGAT AAAGTCTTTC CTGGTGGAAG CCATACATAT 
GTCTGGCAGG TCCTGAAAGA GAATGGTCCA ATGGCCTCTG ACCCGCTGTG CCTTACCTAC 
TCGTATCTTT CTCATGTGGA CCTGGTAAAA GACTTGAATT CGGGCCTCAT TGGAGCCCTA 
CTAGTATGTA GAGAAGGGAG TCTGGCCAAG GAAAAGACAC AGACCTTGCA CAAATTTGTA 
CTACTTTTTG CTGTATTTGA TGAAGGGAAA AGTTGGCACT CAGAAACAAA GAACTCCTTG 
ATGCAGGATA GGGATGATGC ATCTGCTCGG GCCTGGCCTA AAATGCACAC AGTCAATGGT 
TATGTAAACA GGTCTCTACC AGGTCTGATT GGATGCCACA GGAAGTCAGT CTATTGGCAT 
GTGATTGGAA TGGGCACCAC TCCTGAAGTG CACTCAATAT TCCTCGAAGG TCACACATTT 
CTTGTGAGGA ACCATCGCCA GGCCTCCTTG GAAATCTCAC CAATAACTTT CCTTACTGCT 
CAAACACTCC TGATGGACCT TGGACAGTTT CTACTGTTTT GTCATATCTC TTCCCACCAA 
CATGATGGCA TGGAAGCTTA TGTCAAAGTA GACAGTTGCC CAGAGGAACC CCAACTACGA 
ATGAAAAATA ATGAAGAAGC GGAAGACTAT GATGATGATC TTGCTGATTC TGAAATGGAT 
GTGGTCAGGT TTGATGATGA CAACTCTCCT TCCTTTATCC AAATTCGCTC AGTTGCCAAG 
AAGCATCCTA AAACTTGGGT ACATTACATT GCTGCTGAAG AGGAGGTCTG GGACTATGCT 
CCCTCAGTCC TCGCCCCTGA TGACAGAAGT TATAAAAGTC AATATTTGAA CAATGGCTCT 
CAGCGGATTG GTAGGAAGTA CAAAAAAGTC CGATTTATGG CATACACAGA TGAAACCTTT 
AAGACTCGTG AAGCTATTCA GTATGAATCA GGAATCTTGG GACCTTTACT TTATGGGGAA 
GTTGGAGACA CACTGTTGAT TATATTTAAG AATCAAGCAA GCAGACCATA TAACATCTAC 
CCTCACGGAA TCACTGATGT CCGTCCTTTG TATTCAAGGA GATTACCAAA AGGTGTAAAA 
CATTTGAAGG ATTTTCCAAT TCTGCCAGGA GAAATATTCA AATATAAATG GACAGTGACT 
GTAGAAGATG GGCCAACTAA ATCAGATCCT CGGTGCCTGA CCCGTTATTA CTCTAGTTTC 
ATTAATATGG AGAGAGATCT AGCTTCAGGA CTCATTGGCC CTCTCCTCAT CTGCTACAAA 
GAATCTGTAG ATCAAAGAGG AAACCAGATA ATGTCAGACA AGAGGAATGT CATCCTGTTT 
TCTGTTTTTG ATGAGAACCA AAGCTGGTAC CTCACAGAGA ATATACAACG CTTTCTCCCC 
AATCCAGTTG GAGTGCAGCT TGAGGATCCA GAGTTCCAAG CCTCCAACAT CATGCACAGC 
ATCAATGGCT ATGTTTTTGA TAGTCTGCAG TTGTCAGTTT GTTTGCATGA GGTGGCATAC 
TGGTACATTC TAAGCATTGG AGCACAGACT GACTTCCTTT CTGTCTTCTT CTCTGGATAT 
ACCTTCAAAC ACAAAATGGT CTATGAAGAC ACACTTACCC TATTCCCATT CTCAGGAGAA 
ACTGTCTTCA TGTCGATGGA AAACCCAGGC CTATGGATTC TGGGGTGCCA CAACTCAGAC 
TTTCGGAACA GAGGCATGAC CGCCTTACTG AAGGTTTCTA GTTGTGACAA GAACACTGGT 
GATTATTACG AGGACAGTTA TGAAGATATT TCAACATACT TGCTGAGTAA AAACAATGCC 
ATTGAGCCAA GAAGTTTCTC CCAGAATTCA AGACACCCTA GCCCTAGGCA AAAGCAATTT 
AATGCCACCA CAATTCCCAA AAATGACATA GAGAAGACTG ACCCTTGGTT TGCACACAGA 
ACACCAATGC CTAAAGTACA GAATGTCTCC TCTAGTGATT TGCTGATGCT CTTGCGACAG 
AGTCCTACTC CACATGGGCT ATCCTTATCT GATCTCCAAG AAGCCGAATA TGAGACTTTT 
TCTGATGATC CATCACCTGG AGCAATAGAC ACTAATAACA ACCTTTCTAA AATGACACAC 
CTCAGGCCAC AGCCCCATCA CAGTGGGGAC ATGGTATTTA CCCCTGAGCC AGACCTCCAA 
TTAAGATTAA ATGAGAAACT GGGGACAACT GTAGCAACAG AGTTGAAAAA ATTTGATTTT 
AAAGTTTCTA GTTCATCAAA TAATCTGATT TCAACAATTC CGTCAGACAA TTTGGTAGCA 
GGTAGTGATA ATACAAGTTC CTTAGGACCC CCAAATATAC CAGTTCATTA TGAGAGTCAA 
TTAGATACCA CTCTGTCTGG CAAAAAGTCA TCTCCCCTTA TTGAGTCTGG TGGACCTCTG 
AGTTTGAGTG AAGAAAATAA TGATTCAAAG TTGTTAGAAT CAGGTTTAAT GAATAGCCAA 
GAAAGTTCAT GGGGAAAAAA TGTATGGTCA ACAGAGAGTG GTAGGTTATT TAAAGAGAAA 
AGAGCTCATG GACCTGCTTT GTTGACTAAA GATAATGCCT TATTCAAAGT TAGCATCTCT 
TTGTTAAAGA TAAACAAAAC TTCCAATAAT TCAGCAACTA ATAGAAAGAC TCACATTGAT 
GGCCCATCAT TATTAGTTGA GAATAGTCCA TCAGTCTGGC AAAATATATT AGAAAGTGAT 
ACTGAATTTC AAAAAGTGAC ACCTTTGATT CATGACAGAA TGCTTACAGA CAAAAATACT 
ACAGCTTTGA GGCTAAATCA TATGTCAAAT AAAACTACTT CATCGAAAAA CATGGAAATG 
GTCCAACAAA AAATAGAGGG CCCCATACTA CCAGATGCAG AAAATCCAGA TATGTCATTC 
TTTAAGATGC TATTCTTACC AGAATCAGCA AATTGGATAC AAAGGACTCA TGGAAAGAAC 
TCCCTGAACT CGGGGCAAGG CCCCAGTGCA AAACTATTCA TATCCTTAGG ACCAGAAAAC 
TCTGTGGAAG GTCAGAATTT CTTGTCTGAG AAAAATAAAG TGGTAGTAGG AAAGGGTGAA 
CTTACAAAGG ATATAGGACT CAAAGAGATA GTTTTTCCAA GCAGCAGAAA CCTATTTCTT 
ACTAACTTGG ATAATTTACA TGAAAATAAT ACACACAATC AAGAAAAAAA AATTCAGGAA 
GAAATAGAAA GAAAGGAAAC ATTAATCCAA GATAATATAG TTTTGCCTCA GATACATACA 
GTGACTGGCA CTAAGAATTT CATGAAGAAC CTTTTCTTAC TGAGCACTAG GCAAAATGTA 
GAAGGTTCAT ATGAGGGGGC ATATGCTCCA GTACTTCAAG ATTTTAGGTC ATTAAGTGAT 
TCAACAAATA GAACAAAGAA CCACATGGCT CATTTCTCAG AAAAAGGGGA GGAAGAAAAC 
TTGGAAGGTT TGGGAAATCA AACCAAGCAA ATTGTAGAGA AATATCCACA CACCACAAGG 
ATATCTCCTA ATCCAAACCA GCAAAATTTT GTCACGCAAC GTGGTAAGAG AGCTTTGAAA 
CAATTCAGAC TCCCACTAGA AGAAACAGAA CTTGAAAAAA GGCTAATTGT GGAGGAGACC 
TCAACCCAGT GGTCCAAAAA CATAAAACAT TTGACCCCAA GCACCCTCAC ACAGATAGAC 
TACAATGAGA AGGAGAAAGG AGCCATTACT CAGTCTCCCT TATCAGATTG TCTTACGAGG 
AGTCATAGCA TCACTCAAGC AAATAGATCT CCATTACCCA TTGCAAAGGT ATCATCATTT 
CCATCTATTA GACCTATGGA TCTGACCAGG GTCCTATTCC AAGACAACTT TTCTCGTCTT 
CCAGCACCAC CTTACAGAAA GAAAGATTCT GGGGTCCAAG AAAGCAGTCA TTTCTTACAA 
GGAGTCAAAA AAAATAACCT TTCTTTAGCT ATTCTAACCT TGGAGATGAT TGGTGATCAA 
AGAGAGGTTG GCTCCCTGGT GACAAGTGCT ACAAATTCAG TCACGTACAA GAAAGTTGAG 
AACACTGTTT TCTTGAAACC AGGCTTGCCC GAAACATCTG GCAAAGTTGA ATTGCTTCCA 
AAAGTTCGCA TTTATCAGAA GGACCTTTTC CCTACAGAAA CTAGCAGTGG GTCTCCTGGC 
CATCTGGATC TCATGGAAGG GAGCCTTCTT CAGGAAACAG AGGGAGCAAT TAAGTGGAAG 
GAAGCAAATA GACCTGGAAA AATTCCCTTT CTGAGAGGAG CAACAGAAAG CTCTGCAAAG 
ACTCCTTCCA AGCTATTGGA TCCTCTTGCT TGGGATAACC ACTATGGTAC TCAGATACCA 
AAAGAAGAGT GGAAATCCCA AGAGAAGTCA CCAGAAAACA CAGCTTTTAA GAAAAAGGAT 
ACCATTTTGC CCCTGAACCC TTGTGAAAGC AATCATACAA TAGCAGCAAT AAATGAGGAA 
CAAAATGAGC CCCAAATAGA AGTCACCTGG GCAAAGCAAG GTGGGACTGA AAGGCTGTGC 
TCTCAAAACC CACCAGTCTT GAAACGTCAT CAAAGGGAAA TAACTCTTAA TACTCTTCAG 
TCAGATCAAG AGGAAATTGA CTATGATGAT ACCATCTCAG TTGAAATGAA GAAGGAAGAT 
TTTGACATTT ATGGTGAGGA TGAAAATCAG AGCCCCCGCA GCTTTCAAAA GAAAACACGA 
CACTATTTTA TTGCTGCAGT GGAGCGGCTC TGGGATTATG GGATGAGTAG TTCCCCGCAT 
GTTCTAAGAA ACAGGGCTCA GAGTGGCAGT GTCCCTCAGT TCAAGAAAGT TGTTTTCCAG 
GAATTTACTG ATGGCTCCTT TACTCAGCCC TTATACCGTG GAGAACTAAA TGAACATTTG 
GGACTCCTGG GGCCATATAT AAGAGCAGAA GTTGAAGATA ATATCATGGT AACTTTCAAA 
AATCAGGCCT CTCGTCCCTA TTCCTTCTAT TCTAGCCTTA TTTCTTATGA GGAAGATCAG 
AGGCAAGGAG CAGAACCTAG AAAAAATTTT GTCAAGCCTA ATGAAACCAA AACTTACTTT 
TGGAAAGTGC AACATCATAT GGCGCCCACT AAAGATGAGT TTGACTGCAA AGCCTGGGCT 
TATTTCTCTG ATGTTGACCT GGAAAAAGAT GTGCACTCAG GCCTGATTGG ACCCCTTCTG 
GTCTGCCACA CTAACACACT GAACCCTGCT CATGGGAGAC AAGTGACAGT ACAGGAATTT 
GCTCTGTTTT TCACTATCTT TGATGAGACC AAAAGCTGGT ACTTCACTGA AAACACAGAA 
AGAAACTGCA GGGCTCCCTG CAATATCCAG ATGGAAGATC CCACTTTTAA AGAGAATTAT 
CGCTTCCATG CAATCAATGG CTACATAATG GATACACTAC CTGGCTTAGT AATGGCTCAG 
GATCAAAGGA TTCGATGGTA TCTGCTCAGC ATGGGCAGCA ATGAAAACAT CCATTCTATT 
CATTTTAGTG GACATGTGTT CACTGTACGA AAAAAAGAGG AGTATAAAAT GGCAGTGTAC 
AATCTCTATC CAGGTGTTTT TGAGACAGTT GAAATGTTAC CATCCAAAGC TGGAATTTGG 
CGGGTGGAAT GCCTTATTGG CGAGCACCTA CATGCTGGGA TGAGCACACT TTTTCTGGTG 
TACAGCAACA AGTGTCAGAC TCCCCTGGGA ATGGCTTCTG GACGCATTAG AGATTTTCAG 
ATTACAGCTT CAGGACAATA TGGACAGTGG GCCCCAAAGC TGGCCAGACT TCATTATTCT 
GGATCAATCA ATGCCTGGAG CACCAAGGAG CCCTTTTCTT GGATCAAGGT GGATCTGTTG 
GCACCAATGA TTATTCACGG CATCAAGACC CAGGGTGCCC GTCAGAAGTT CTCCAGCCTC 
TACATCTCTC AGTTTATCAT CATGTATAGT CTTGATGGGA AAAAGTGGCA GACTTATCGA 
GGAAATTCCA CTGGAACCTT AATGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA 
CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT 
AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG 
CCGTTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTC 
ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTAACCTCCA AGGAAGGAGT 
AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG 
ACAATGAAAG TCACAGGAAT AACTACTCAG GGAGTAAAAT CTCTACTTAC CAACATGTAT 
GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC ACTGGACTCT CTTCTTTCAG 
AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT 
CTAGACTCAC CGTTACTGAC TCGCTACCTT CGAATTCATC CCCAGAGTTG GGTGCACCAG 
ATTGCCCTGA GGATAGAGGT TCTGGGCTGC GAGGCACAGG AGCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014984060.2
CDS	1020..7970
Translation	MQSDLGELPVDTRFPPRVPRSFPFNTSVMYKKTVFVEFTDHLFNIAKPRPPWMGLLGPTIQAEVYDTVVITLKNMASHPVSLHAVGVSYWKASEGAEYDDQTSQREKEDDKVFPGGSHTYVWQVLKENGPMASDPLCLTYSYLSHVDLVKDLNSGLIGALLVCREGSLAKEKTQTLHKFVLLFAVFDEGKSWHSETKNSLMQDRDDASARAWPKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTLLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKNNEEAEDYDDDLADSEMDVVRFDDDNSPSFIQIRSVAKKHPKTWVHYIAAEEEVWDYAPSVLAPDDRSYKSQYLNNGSQRIGRKYKKVRFMAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVRPLYSRRLPKGVKHLKDFPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINMERDLASGLIGPLLICYKESVDQRGNQIMSDKRNVILFSVFDENQSWYLTENIQRFLPNPVGVQLEDPEFQASNIMHSINGYVFDSLQLSVCLHEVAYWYILSIGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISTYLLSKNNAIEPRSFSQNSRHPSPRQKQFNATTIPKNDIEKTDPWFAHRTPMPKVQNVSSSDLLMLLRQSPTPHGLSLSDLQEAEYETFSDDPSPGAIDTNNNLSKMTHLRPQPHHSGDMVFTPEPDLQLRLNEKLGTTVATELKKFDFKVSSSSNNLISTIPSDNLVAGSDNTSSLGPPNIPVHYESQLDTTLSGKKSSPLIESGGPLSLSEENNDSKLLESGLMNSQESSWGKNVWSTESGRLFKEKRAHGPALLTKDNALFKVSISLLKINKTSNNSATNRKTHIDGPSLLVENSPSVWQNILESDTEFQKVTPLIHDRMLTDKNTTALRLNHMSNKTTSSKNMEMVQQKIEGPILPDAENPDMSFFKMLFLPESANWIQRTHGKNSLNSGQGPSAKLFISLGPENSVEGQNFLSEKNKVVVGKGELTKDIGLKEIVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIERKETLIQDNIVLPQIHTVTGTKNFMKNLFLLSTRQNVEGSYEGAYAPVLQDFRSLSDSTNRTKNHMAHFSEKGEEENLEGLGNQTKQIVEKYPHTTRISPNPNQQNFVTQRGKRALKQFRLPLEETELEKRLIVEETSTQWSKNIKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSITQANRSPLPIAKVSSFPSIRPMDLTRVLFQDNFSRLPAPPYRKKDSGVQESSHFLQGVKKNNLSLAILTLEMIGDQREVGSLVTSATNSVTYKKVENTVFLKPGLPETSGKVELLPKVRIYQKDLFPTETSSGSPGHLDLMEGSLLQETEGAIKWKEANRPGKIPFLRGATESSAKTPSKLLDPLAWDNHYGTQIPKEEWKSQEKSPENTAFKKKDTILPLNPCESNHTIAAINEEQNEPQIEVTWAKQGGTERLCSQNPPVLKRHQREITLNTLQSDQEEIDYDDTISVEMKKEDFDIYGEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSGSVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFDETKSWYFTENTERNCRAPCNIQMEDPTFKENYRFHAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMAVYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLNLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTNMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDSPLLTRYLRIHPQSWVHQIALRIEVLGCEAQELY

Target ORF information:

RefSeq Version	XM_015128574.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta coagulation factor VIII (F8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015128574.2

ORF Insert Sequence:

ATGCAAAGTG ATCTCGGTGA GCTACCTGTG GACACAAGAT TTCCTCCTAG AGTGCCAAGA 
TCTTTTCCAT TCAACACCTC AGTCATGTAC AAAAAGACTG TGTTTGTAGA ATTCACGGAT 
CACCTTTTCA ACATCGCTAA GCCAAGGCCA CCTTGGATGG GTCTGCTGGG TCCTACTATC 
CAGGCTGAGG TTTATGACAC AGTGGTCATT ACACTTAAGA ACATGGCTTC CCATCCTGTC 
AGTCTTCATG CTGTTGGTGT TTCCTACTGG AAAGCTTCTG AGGGAGCTGA ATATGATGAT 
CAGACCAGTC AAAGGGAGAA AGAAGATGAT AAAGTCTTTC CTGGTGGAAG CCATACATAT 
GTCTGGCAGG TCCTGAAAGA GAATGGTCCA ATGGCCTCTG ACCCGCTGTG CCTTACCTAC 
TCGTATCTTT CTCATGTGGA CCTGGTAAAA GACTTGAATT CGGGCCTCAT TGGAGCCCTA 
CTAGTATGTA GAGAAGGGAG TCTGGCCAAG GAAAAGACAC AGACCTTGCA CAAATTTGTA 
CTACTTTTTG CTGTATTTGA TGAAGGGAAA AGTTGGCACT CAGAAACAAA GAACTCCTTG 
ATGCAGGATA GGGATGATGC ATCTGCTCGG GCCTGGCCTA AAATGCACAC AGTCAATGGT 
TATGTAAACA GGTCTCTACC AGGTCTGATT GGATGCCACA GGAAGTCAGT CTATTGGCAT 
GTGATTGGAA TGGGCACCAC TCCTGAAGTG CACTCAATAT TCCTCGAAGG TCACACATTT 
CTTGTGAGGA ACCATCGCCA GGCCTCCTTG GAAATCTCAC CAATAACTTT CCTTACTGCT 
CAAACACTCC TGATGGACCT TGGACAGTTT CTACTGTTTT GTCATATCTC TTCCCACCAA 
CATGATGGCA TGGAAGCTTA TGTCAAAGTA GACAGTTGCC CAGAGGAACC CCAACTACGA 
ATGAAAAATA ATGAAGAAGC GGAAGACTAT GATGATGATC TTGCTGATTC TGAAATGGAT 
GTGGTCAGGT TTGATGATGA CAACTCTCCT TCCTTTATCC AAATTCGCTC AGTTGCCAAG 
AAGCATCCTA AAACTTGGGT ACATTACATT GCTGCTGAAG AGGAGGTCTG GGACTATGCT 
CCCTCAGTCC TCGCCCCTGA TGACAGAAGT TATAAAAGTC AATATTTGAA CAATGGCTCT 
CAGCGGATTG GTAGGAAGTA CAAAAAAGTC CGATTTATGG CATACACAGA TGAAACCTTT 
AAGACTCGTG AAGCTATTCA GTATGAATCA GGAATCTTGG GACCTTTACT TTATGGGGAA 
GTTGGAGACA CACTGTTGAT TATATTTAAG AATCAAGCAA GCAGACCATA TAACATCTAC 
CCTCACGGAA TCACTGATGT CCGTCCTTTG TATTCAAGGA GATTACCAAA AGGTGTAAAA 
CATTTGAAGG ATTTTCCAAT TCTGCCAGGA GAAATATTCA AATATAAATG GACAGTGACT 
GTAGAAGATG GGCCAACTAA ATCAGATCCT CGGTGCCTGA CCCGTTATTA CTCTAGTTTC 
ATTAATATGG AGAGAGATCT AGCTTCAGGA CTCATTGGCC CTCTCCTCAT CTGCTACAAA 
GAATCTGTAG ATCAAAGAGG AAACCAGATA ATGTCAGACA AGAGGAATGT CATCCTGTTT 
TCTGTTTTTG ATGAGAACCA AAGCTGGTAC CTCACAGAGA ATATACAACG CTTTCTCCCC 
AATCCAGTTG GAGTGCAGCT TGAGGATCCA GAGTTCCAAG CCTCCAACAT CATGCACAGC 
ATCAATGGCT ATGTTTTTGA TAGTCTGCAG TTGTCAGTTT GTTTGCATGA GGTGGCATAC 
TGGTACATTC TAAGCATTGG AGCACAGACT GACTTCCTTT CTGTCTTCTT CTCTGGATAT 
ACCTTCAAAC ACAAAATGGT CTATGAAGAC ACACTTACCC TATTCCCATT CTCAGGAGAA 
ACTGTCTTCA TGTCGATGGA AAACCCAGGC CTATGGATTC TGGGGTGCCA CAACTCAGAC 
TTTCGGAACA GAGGCATGAC CGCCTTACTG AAGGTTTCTA GTTGTGACAA GAACACTGGT 
GATTATTACG AGGACAGTTA TGAAGATATT TCAACATACT TGCTGAGTAA AAACAATGCC 
ATTGAGCCAA GAAGTTTCTC CCAGAATTCA AGACACCCTA GCCCTAGGCA AAAGCAATTT 
AATGCCACCA CAATTCCCAA AAATGACATA GAGAAGACTG ACCCTTGGTT TGCACACAGA 
ACACCAATGC CTAAAGTACA GAATGTCTCC TCTAGTGATT TGCTGATGCT CTTGCGACAG 
AGTCCTACTC CACATGGGCT ATCCTTATCT GATCTCCAAG AAGCCGAATA TGAGACTTTT 
TCTGATGATC CATCACCTGG AGCAATAGAC ACTAATAACA ACCTTTCTAA AATGACACAC 
CTCAGGCCAC AGCCCCATCA CAGTGGGGAC ATGGTATTTA CCCCTGAGCC AGACCTCCAA 
TTAAGATTAA ATGAGAAACT GGGGACAACT GTAGCAACAG AGTTGAAAAA ATTTGATTTT 
AAAGTTTCTA GTTCATCAAA TAATCTGATT TCAACAATTC CGTCAGACAA TTTGGTAGCA 
GGTAGTGATA ATACAAGTTC CTTAGGACCC CCAAATATAC CAGTTCATTA TGAGAGTCAA 
TTAGATACCA CTCTGTCTGG CAAAAAGTCA TCTCCCCTTA TTGAGTCTGG TGGACCTCTG 
AGTTTGAGTG AAGAAAATAA TGATTCAAAG TTGTTAGAAT CAGGTTTAAT GAATAGCCAA 
GAAAGTTCAT GGGGAAAAAA TGTATGGTCA ACAGAGAGTG GTAGGTTATT TAAAGAGAAA 
AGAGCTCATG GACCTGCTTT GTTGACTAAA GATAATGCCT TATTCAAAGT TAGCATCTCT 
TTGTTAAAGA TAAACAAAAC TTCCAATAAT TCAGCAACTA ATAGAAAGAC TCACATTGAT 
GGCCCATCAT TATTAGTTGA GAATAGTCCA TCAGTCTGGC AAAATATATT AGAAAGTGAT 
ACTGAATTTC AAAAAGTGAC ACCTTTGATT CATGACAGAA TGCTTACAGA CAAAAATACT 
ACAGCTTTGA GGCTAAATCA TATGTCAAAT AAAACTACTT CATCGAAAAA CATGGAAATG 
GTCCAACAAA AAATAGAGGG CCCCATACTA CCAGATGCAG AAAATCCAGA TATGTCATTC 
TTTAAGATGC TATTCTTACC AGAATCAGCA AATTGGATAC AAAGGACTCA TGGAAAGAAC 
TCCCTGAACT CGGGGCAAGG CCCCAGTGCA AAACTATTCA TATCCTTAGG ACCAGAAAAC 
TCTGTGGAAG GTCAGAATTT CTTGTCTGAG AAAAATAAAG TGGTAGTAGG AAAGGGTGAA 
CTTACAAAGG ATATAGGACT CAAAGAGATA GTTTTTCCAA GCAGCAGAAA CCTATTTCTT 
ACTAACTTGG ATAATTTACA TGAAAATAAT ACACACAATC AAGAAAAAAA AATTCAGGAA 
GAAATAGAAA GAAAGGAAAC ATTAATCCAA GATAATATAG TTTTGCCTCA GATACATACA 
GTGACTGGCA CTAAGAATTT CATGAAGAAC CTTTTCTTAC TGAGCACTAG GCAAAATGTA 
GAAGGTTCAT ATGAGGGGGC ATATGCTCCA GTACTTCAAG ATTTTAGGTC ATTAAGTGAT 
TCAACAAATA GAACAAAGAA CCACATGGCT CATTTCTCAG AAAAAGGGGA GGAAGAAAAC 
TTGGAAGGTT TGGGAAATCA AACCAAGCAA ATTGTAGAGA AATATCCACA CACCACAAGG 
ATATCTCCTA ATCCAAACCA GCAAAATTTT GTCACGCAAC GTGGTAAGAG AGCTTTGAAA 
CAATTCAGAC TCCCACTAGA AGAAACAGAA CTTGAAAAAA GGCTAATTGT GGAGGAGACC 
TCAACCCAGT GGTCCAAAAA CATAAAACAT TTGACCCCAA GCACCCTCAC ACAGATAGAC 
TACAATGAGA AGGAGAAAGG AGCCATTACT CAGTCTCCCT TATCAGATTG TCTTACGAGG 
AGTCATAGCA TCACTCAAGC AAATAGATCT CCATTACCCA TTGCAAAGGT ATCATCATTT 
CCATCTATTA GACCTATGGA TCTGACCAGG GTCCTATTCC AAGACAACTT TTCTCGTCTT 
CCAGCACCAC CTTACAGAAA GAAAGATTCT GGGGTCCAAG AAAGCAGTCA TTTCTTACAA 
GGAGTCAAAA AAAATAACCT TTCTTTAGCT ATTCTAACCT TGGAGATGAT TGGTGATCAA 
AGAGAGGTTG GCTCCCTGGT GACAAGTGCT ACAAATTCAG TCACGTACAA GAAAGTTGAG 
AACACTGTTT TCTTGAAACC AGGCTTGCCC GAAACATCTG GCAAAGTTGA ATTGCTTCCA 
AAAGTTCGCA TTTATCAGAA GGACCTTTTC CCTACAGAAA CTAGCAGTGG GTCTCCTGGC 
CATCTGGATC TCATGGAAGG GAGCCTTCTT CAGGAAACAG AGGGAGCAAT TAAGTGGAAG 
GAAGCAAATA GACCTGGAAA AATTCCCTTT CTGAGAGGAG CAACAGAAAG CTCTGCAAAG 
ACTCCTTCCA AGCTATTGGA TCCTCTTGCT TGGGATAACC ACTATGGTAC TCAGATACCA 
AAAGAAGAGT GGAAATCCCA AGAGAAGTCA CCAGAAAACA CAGCTTTTAA GAAAAAGGAT 
ACCATTTTGC CCCTGAACCC TTGTGAAAGC AATCATACAA TAGCAGCAAT AAATGAGGAA 
CAAAATGAGC CCCAAATAGA AGTCACCTGG GCAAAGCAAG GTGGGACTGA AAGGCTGTGC 
TCTCAAAACC CACCAGTCTT GAAACGTCAT CAAAGGGAAA TAACTCTTAA TACTCTTCAG 
TCAGATCAAG AGGAAATTGA CTATGATGAT ACCATCTCAG TTGAAATGAA GAAGGAAGAT 
TTTGACATTT ATGGTGAGGA TGAAAATCAG AGCCCCCGCA GCTTTCAAAA GAAAACACGA 
CACTATTTTA TTGCTGCAGT GGAGCGGCTC TGGGATTATG GGATGAGTAG TTCCCCGCAT 
GTTCTAAGAA ACAGGGCTCA GAGTGGCAGT GTCCCTCAGT TCAAGAAAGT TGTTTTCCAG 
GAATTTACTG ATGGCTCCTT TACTCAGCCC TTATACCGTG GAGAACTAAA TGAACATTTG 
GGACTCCTGG GGCCATATAT AAGAGCAGAA GTTGAAGATA ATATCATGGT AACTTTCAAA 
AATCAGGCCT CTCGTCCCTA TTCCTTCTAT TCTAGCCTTA TTTCTTATGA GGAAGATCAG 
AGGCAAGGAG CAGAACCTAG AAAAAATTTT GTCAAGCCTA ATGAAACCAA AACTTACTTT 
TGGAAAGTGC AACATCATAT GGCGCCCACT AAAGATGAGT TTGACTGCAA AGCCTGGGCT 
TATTTCTCTG ATGTTGACCT GGAAAAAGAT GTGCACTCAG GCCTGATTGG ACCCCTTCTG 
GTCTGCCACA CTAACACACT GAACCCTGCT CATGGGAGAC AAGTGACAGT ACAGGAATTT 
GCTCTGTTTT TCACTATCTT TGATGAGACC AAAAGCTGGT ACTTCACTGA AAACACAGAA 
AGAAACTGCA GGGCTCCCTG CAATATCCAG ATGGAAGATC CCACTTTTAA AGAGAATTAT 
CGCTTCCATG CAATCAATGG CTACATAATG GATACACTAC CTGGCTTAGT AATGGCTCAG 
GATCAAAGGA TTCGATGGTA TCTGCTCAGC ATGGGCAGCA ATGAAAACAT CCATTCTATT 
CATTTTAGTG GACATGTGTT CACTGTACGA AAAAAAGAGG AGTATAAAAT GGCAGTGTAC 
AATCTCTATC CAGGTGTTTT TGAGACAGTT GAAATGTTAC CATCCAAAGC TGGAATTTGG 
CGGGTGGAAT GCCTTATTGG CGAGCACCTA CATGCTGGGA TGAGCACACT TTTTCTGGTG 
TACAGCAACA AGTGTCAGAC TCCCCTGGGA ATGGCTTCTG GACGCATTAG AGATTTTCAG 
ATTACAGCTT CAGGACAATA TGGACAGTGG GCCCCAAAGC TGGCCAGACT TCATTATTCT 
GGATCAATCA ATGCCTGGAG CACCAAGGAG CCCTTTTCTT GGATCAAGGT GGATCTGTTG 
GCACCAATGA TTATTCACGG CATCAAGACC CAGGGTGCCC GTCAGAAGTT CTCCAGCCTC 
TACATCTCTC AGTTTATCAT CATGTATAGT CTTGATGGGA AAAAGTGGCA GACTTATCGA 
GGAAATTCCA CTGGAACCTT AATGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA 
CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT 
AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG 
CCGTTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTC 
ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTAACCTCCA AGGAAGGAGT 
AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG 
ACAATGAAAG TCACAGGAAT AACTACTCAG GGAGTAAAAT CTCTACTTAC CAACATGTAT 
GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC ACTGGACTCT CTTCTTTCAG 
AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT 
CTAGACTCAC CGTTACTGAC TCGCTACCTT CGAATTCATC CCCAGAGTTG GGTGCACCAG 
ATTGCCCTGA GGATAGAGGT TCTGGGCTGC GAGGCACAGG AGCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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F8 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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