F8 cDNA ORF clone, Homo sapiens(human)

The following F8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23554 NM_019863.2
Latest version!
Homo sapiens coagulation factor VIII (F8), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu23783 NM_000132.3
Latest version!
Homo sapiens coagulation factor VIII (F8), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$929.50-$1301.30
$1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu23554
    Clone ID Related Accession (Same CDS sequence) NM_019863.2
    Accession Version NM_019863.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product coagulation factor VIII isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI547023.1, BC064380.1 and BC022513.1. On May 19, 2005 this sequence version replaced NM_019863.1. Transcript Variant: This variant (2) contains an unique 5' exon located within intron 22 of transcript variant 1. This exon codes for eight amino acids and is spliced to exons 23-26 maintaining the reading frame. The resulting isoform (b) is considerably shorter compared to isoform a, and includes the phospholipid binding domain. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098389.1, BC064380.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGCGGATCC AAGACCCTGG GAAGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA 
    CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT
    AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG
    CCATTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTT
    ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTCACCTCCA AGGGAGGAGT
    AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG
    ACAATGAAAG TCACAGGAGT AACTACTCAG GGAGTAAAAT CTCTGCTTAC CAGCATGTAT
    GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC AGTGGACTCT CTTTTTTCAG
    AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT
    CTAGACCCAC CGTTACTGAC TCGCTACCTT CGAATTCACC CCCAGAGTTG GGTGCACCAG
    ATTGCCCTGA GGATGGAGGT TCTGGGCTGC GAGGCACAGG ACCTCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_063916.1
    CDS146..796
    Translation

    Target ORF information:

    RefSeq Version NM_019863.2
    Organism Homo sapiens(human)
    Definition Homo sapiens coagulation factor VIII (F8), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_019863.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGCGGATCC AAGACCCTGG GAAGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA 
    CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT
    AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG
    CCATTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTT
    ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTCACCTCCA AGGGAGGAGT
    AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG
    ACAATGAAAG TCACAGGAGT AACTACTCAG GGAGTAAAAT CTCTGCTTAC CAGCATGTAT
    GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC AGTGGACTCT CTTTTTTCAG
    AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT
    CTAGACCCAC CGTTACTGAC TCGCTACCTT CGAATTCACC CCCAGAGTTG GGTGCACCAG
    ATTGCCCTGA GGATGGAGGT TCTGGGCTGC GAGGCACAGG ACCTCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23783
    Clone ID Related Accession (Same CDS sequence) NM_000132.3
    Accession Version NM_000132.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product coagulation factor VIII isoform a preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M14113.1 and BC022513.1. This sequence is a reference standard in the RefSeqGene project. On Jun 24, 2008 this sequence version replaced NM_000132.2. Transcript Variant: This variant (1) consists of 26 exons and encodes the full-length isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M14113.1, K01740.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAAATAG AGCTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
    ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC
    GGTGAGCTGC CTGTGGACGC AAGATTTCCT CCTAGAGTGC CAAAATCTTT TCCATTCAAC
    ACCTCAGTCG TGTACAAAAA GACTCTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC
    GCTAAGCCAA GGCCACCCTG GATGGGTCTG CTAGGTCCTA CCATCCAGGC TGAGGTTTAT
    GATACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT
    GGTGTATCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG
    GAGAAAGAAG ATGATAAAGT CTTCCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG
    AAAGAGAATG GTCCAATGGC CTCTGACCCA CTGTGCCTTA CCTACTCATA TCTTTCTCAT
    GTGGACCTGG TAAAAGACTT GAATTCAGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA
    GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTATACTACT TTTTGCTGTA
    TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT
    GCTGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT
    CTGCCAGGTC TGATTGGATG CCACAGGAAA TCAGTCTATT GGCATGTGAT TGGAATGGGC
    ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT
    CGCCAGGCGT CCTTGGAAAT CTCGCCAATA ACTTTCCTTA CTGCTCAAAC ACTCTTGATG
    GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA
    GCTTATGTCA AAGTAGACAG CTGTCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA
    GAAGCGGAAG ACTATGATGA TGATCTTACT GATTCTGAAA TGGATGTGGT CAGGTTTGAT
    GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT
    TGGGTACATT ACATTGCTGC TGAAGAGGAG GACTGGGACT ATGCTCCCTT AGTCCTCGCC
    CCCGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCCCTCAGCG GATTGGTAGG
    AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT
    ATTCAGCATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG
    TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT
    GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT
    CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA
    ACTAAATCAG ATCCTCGGTG CCTGACCCGC TATTACTCTA GTTTCGTTAA TATGGAGAGA
    GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT ATTTGATGAG
    AACCGAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGCTGGAGTG
    CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT
    TTTGATAGTT TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC
    ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA
    ATGGTCTATG AAGACACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG
    ATGGAAAACC CAGGTCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC
    ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC
    AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC
    TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT
    CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA
    ATACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT
    GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA
    CCTGGAGCAA TAGACAGTAA TAACAGCCTG TCTGAAATGA CACACTTCAG GCCACAGCTC
    CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTAAG ATTAAATGAG
    AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA
    TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA
    AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA
    TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTGGAC CTCTGAGCTT GAGTGAAGAA
    AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA
    AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT
    GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC
    AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA
    ATTGAGAATA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA
    GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA
    AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCA ACAGAAAAAA
    GAGGGCCCCA TTCCACCAGA TGCACAAAAT CCAGATATGT CGTTCTTTAA GATGCTATTC
    TTGCCAGAAT CAGCAAGGTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG
    CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG
    AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA
    GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT
    TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAAGAAG
    GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG
    AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAC
    GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA
    AAGAAACACA CAGCTCATTT CTCAAAAAAA GGGGAGGAAG AAAACTTGGA AGGCTTGGGA
    AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA
    AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA
    CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC
    AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG
    AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT
    CAAGCAAATA GATCTCCATT ACCCATTGCA AAGGTATCAT CATTTCCATC TATTAGACCT
    ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT
    AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT
    AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC
    CTGGGGACAA GTGCCACAAA TTCAGTCACA TACAAGAAAG TTGAGAACAC TGTTCTCCCG
    AAACCAGACT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT
    CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG
    GAAGGGAGCC TTCTTCAGGG AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT
    GGAAAAGTTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA
    TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA
    TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG
    AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA
    ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA
    GTCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA
    ATTGACTATG ATGATACCAT ATCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT
    GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT
    GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG
    GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC
    TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA
    TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAGAAATCA GGCCTCTCGT
    CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA
    CCTAGAAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT
    CATATGGCAC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT
    GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC
    ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC
    ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAATA TGGAAAGAAA CTGCAGGGCT
    CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC
    AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA
    TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT
    GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT
    GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT
    ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT
    CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA
    CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC
    TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT
    CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT
    ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA
    ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC
    CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT
    CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG
    AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC
    ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT
    CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA
    GGAGTAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC
    ATCTCCAGCA GTCAAGATGG CCATCAGTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG
    GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA
    CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG
    GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000123.1
    CDS172..7227
    Translation

    Target ORF information:

    RefSeq Version NM_000132.3
    Organism Homo sapiens(human)
    Definition Homo sapiens coagulation factor VIII (F8), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000132.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAAATAG AGCTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
    ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC
    GGTGAGCTGC CTGTGGACGC AAGATTTCCT CCTAGAGTGC CAAAATCTTT TCCATTCAAC
    ACCTCAGTCG TGTACAAAAA GACTCTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC
    GCTAAGCCAA GGCCACCCTG GATGGGTCTG CTAGGTCCTA CCATCCAGGC TGAGGTTTAT
    GATACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT
    GGTGTATCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG
    GAGAAAGAAG ATGATAAAGT CTTCCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG
    AAAGAGAATG GTCCAATGGC CTCTGACCCA CTGTGCCTTA CCTACTCATA TCTTTCTCAT
    GTGGACCTGG TAAAAGACTT GAATTCAGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA
    GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTATACTACT TTTTGCTGTA
    TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT
    GCTGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT
    CTGCCAGGTC TGATTGGATG CCACAGGAAA TCAGTCTATT GGCATGTGAT TGGAATGGGC
    ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT
    CGCCAGGCGT CCTTGGAAAT CTCGCCAATA ACTTTCCTTA CTGCTCAAAC ACTCTTGATG
    GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA
    GCTTATGTCA AAGTAGACAG CTGTCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA
    GAAGCGGAAG ACTATGATGA TGATCTTACT GATTCTGAAA TGGATGTGGT CAGGTTTGAT
    GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT
    TGGGTACATT ACATTGCTGC TGAAGAGGAG GACTGGGACT ATGCTCCCTT AGTCCTCGCC
    CCCGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCCCTCAGCG GATTGGTAGG
    AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT
    ATTCAGCATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG
    TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT
    GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT
    CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA
    ACTAAATCAG ATCCTCGGTG CCTGACCCGC TATTACTCTA GTTTCGTTAA TATGGAGAGA
    GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT ATTTGATGAG
    AACCGAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGCTGGAGTG
    CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT
    TTTGATAGTT TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC
    ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA
    ATGGTCTATG AAGACACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG
    ATGGAAAACC CAGGTCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC
    ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC
    AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC
    TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT
    CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA
    ATACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT
    GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA
    CCTGGAGCAA TAGACAGTAA TAACAGCCTG TCTGAAATGA CACACTTCAG GCCACAGCTC
    CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTAAG ATTAAATGAG
    AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA
    TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA
    AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA
    TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTGGAC CTCTGAGCTT GAGTGAAGAA
    AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA
    AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT
    GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC
    AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA
    ATTGAGAATA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA
    GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA
    AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCA ACAGAAAAAA
    GAGGGCCCCA TTCCACCAGA TGCACAAAAT CCAGATATGT CGTTCTTTAA GATGCTATTC
    TTGCCAGAAT CAGCAAGGTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG
    CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG
    AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA
    GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT
    TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAAGAAG
    GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG
    AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAC
    GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA
    AAGAAACACA CAGCTCATTT CTCAAAAAAA GGGGAGGAAG AAAACTTGGA AGGCTTGGGA
    AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA
    AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA
    CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC
    AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG
    AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT
    CAAGCAAATA GATCTCCATT ACCCATTGCA AAGGTATCAT CATTTCCATC TATTAGACCT
    ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT
    AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT
    AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC
    CTGGGGACAA GTGCCACAAA TTCAGTCACA TACAAGAAAG TTGAGAACAC TGTTCTCCCG
    AAACCAGACT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT
    CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG
    GAAGGGAGCC TTCTTCAGGG AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT
    GGAAAAGTTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA
    TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA
    TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG
    AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA
    ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA
    GTCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA
    ATTGACTATG ATGATACCAT ATCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT
    GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT
    GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG
    GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC
    TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA
    TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAGAAATCA GGCCTCTCGT
    CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA
    CCTAGAAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT
    CATATGGCAC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT
    GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC
    ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC
    ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAATA TGGAAAGAAA CTGCAGGGCT
    CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC
    AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA
    TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT
    GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT
    GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT
    ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT
    CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA
    CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC
    TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT
    CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT
    ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA
    ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC
    CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT
    CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG
    AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC
    ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT
    CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA
    GGAGTAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC
    ATCTCCAGCA GTCAAGATGG CCATCAGTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG
    GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA
    CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG
    GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.