F8 gene cDNA ORF clone, Pan troglodytes(chimpanzee)

mRNA	Protein	Name
XM_003317788.3	XP_003317836.2	coagulation factor VIII isoform X7
XM_016944625.1	XP_016800114.1	coagulation factor VIII isoform X3
XM_016944627.2	XP_016800116.1	coagulation factor VIII isoform X6
XM_003317789.3	XP_003317837.1	coagulation factor VIII isoform X1
XM_016944626.1	XP_016800115.1	coagulation factor VIII isoform X5
XM_016944625.2	XP_016800114.1	coagulation factor VIII isoform X3
XM_009439870.2	XP_009438145.1	coagulation factor VIII isoform X4
XM_016944627.1	XP_016800116.1	coagulation factor VIII isoform X6
XM_003317789.4	XP_003317837.1	coagulation factor VIII isoform X1
XM_016944624.1	XP_016800113.1	coagulation factor VIII isoform X2
XM_016944626.2	XP_016800115.1	coagulation factor VIII isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc91299	XM_016944627.2 Latest version!	Pan troglodytes coagulation factor VIII (F8), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OPc91298	XM_016944626.2 Latest version!	Pan troglodytes coagulation factor VIII (F8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc91296	XM_016944624.1 Latest version!	Pan troglodytes coagulation factor VIII (F8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc91297	XM_016944625.2 Latest version!	Pan troglodytes coagulation factor VIII (F8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc25432	XM_003317789.4 Latest version!	Pan troglodytes coagulation factor VIII (F8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OPc46412	XM_009439870.2 Latest version!	Pan troglodytes coagulation factor VIII (F8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OPc91300	XM_003317788.3 Latest version!	Pan troglodytes coagulation factor VIII (F8), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
View Old Accession Versions >>
OPc91297	XM_016944625.1	Pan troglodytes coagulation factor VIII (F8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc91299	XM_016944627.1	Pan troglodytes coagulation factor VIII (F8), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OPc25432	XM_003317789.3	Pan troglodytes coagulation factor VIII (F8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OPc91298	XM_016944626.1	Pan troglodytes coagulation factor VIII (F8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

F8 ( XM_016944627.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016800116.1 Pan troglodytes coagulation factor VIII (F8), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPc91299
Clone ID Related Accession (Same CDS sequence)	XM_016944627.2 , XM_016944627.1
Accession Version	XM_016944627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	coagulation factor VIII isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016944627.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACCAT CCAAAGCTGG AATTTGGCGG GTGGAATGCC TTATTGGCGA GCATCTACAT 
GCTGGGATGA GCACACTTTT TCTGGTGTAC AGCAATAAGT GTCAGACTCC CCTGGGAATG 
GCTTCTGGAC ACATTAGAGA TTTTCAGATT ACAGCTTCAG GACAATATGG ACAGTGGGCC 
CCAAAGCTGG CCAGACTTCA TTATTCCGGA TCAATCAATG CCTGGAGCAC CAAGGAGCCC 
TTTTCTTGGA TCAAGGTGGA TCTGTTGGCA CCAATGATTA TTCACGGCAT CAAGACCCAG 
GGTGCCCGTC AGAAGTTCTC CAGCCTCTAC ATCTCTCAGT TTATCATCAT GTATAGTCTT 
GATGGGAAGA AGTGGCAGAC TTATCGAGGA AATTCCACTG GAACCTTAAT GGTCTTCTTT 
GGCAATGTGG ATTCATCTGG GATAAAACAC AATATTTTTA ACCCTCCAAT TATTGCTCGA 
TACATCCGTT TGCACCCAAC TCATTATAGC ATTCGCAGCA CTCTTCGCAT GGAGTTGATG 
GGCTGTGATT TAAATAGTTG CAGCATGCCA TTGGGAATGG AGAGTAAAGC AATATCAGAT 
GCACAGATTA CTGCTTCATC CTACTTTACC AATATGTTTG CCACCTGGTC TCCTTCAAAA 
GCTCGACTTC ACCTCCAAGG GAGGAGTAAT GCCTGGAGAC CTCAGGTGAA TAATCCAAAA 
GAGTGGCTGC AAGTGGACTT CCAGAAGACA ATGAAAGTCA CAGGAATAAC TACTCAGGGA 
GTAAAATCTC TGCTTACCAG CATGTATGTG AAGGAGTTCC TCATCTCCAG CAGTCAAGAT 
GGCCATCACT GGACTCTCTT TTTTCAGAAT GGCAAAGTAA AGGTTTTTCA GGGAAATCAA 
GACTCCTTCA CACCTGTGGT GAACTCTCTA GACCCACCGT TACTGACTCG CTACCTTCGA 
ATTCACCCCC AGAGTTGGGT GCACCAGATT GCCCTGAGGA TGGAGGTTCT GGGCTGCGAG 
GCACAGGACC TCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016800116.1
CDS	426..1463
Translation	MLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGHIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTSMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY

Target ORF information:

RefSeq Version	XM_016944627.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes coagulation factor VIII (F8), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016944627.2

ORF Insert Sequence:

ATGTTACCAT CCAAAGCTGG AATTTGGCGG GTGGAATGCC TTATTGGCGA GCATCTACAT 
GCTGGGATGA GCACACTTTT TCTGGTGTAC AGCAATAAGT GTCAGACTCC CCTGGGAATG 
GCTTCTGGAC ACATTAGAGA TTTTCAGATT ACAGCTTCAG GACAATATGG ACAGTGGGCC 
CCAAAGCTGG CCAGACTTCA TTATTCCGGA TCAATCAATG CCTGGAGCAC CAAGGAGCCC 
TTTTCTTGGA TCAAGGTGGA TCTGTTGGCA CCAATGATTA TTCACGGCAT CAAGACCCAG 
GGTGCCCGTC AGAAGTTCTC CAGCCTCTAC ATCTCTCAGT TTATCATCAT GTATAGTCTT 
GATGGGAAGA AGTGGCAGAC TTATCGAGGA AATTCCACTG GAACCTTAAT GGTCTTCTTT 
GGCAATGTGG ATTCATCTGG GATAAAACAC AATATTTTTA ACCCTCCAAT TATTGCTCGA 
TACATCCGTT TGCACCCAAC TCATTATAGC ATTCGCAGCA CTCTTCGCAT GGAGTTGATG 
GGCTGTGATT TAAATAGTTG CAGCATGCCA TTGGGAATGG AGAGTAAAGC AATATCAGAT 
GCACAGATTA CTGCTTCATC CTACTTTACC AATATGTTTG CCACCTGGTC TCCTTCAAAA 
GCTCGACTTC ACCTCCAAGG GAGGAGTAAT GCCTGGAGAC CTCAGGTGAA TAATCCAAAA 
GAGTGGCTGC AAGTGGACTT CCAGAAGACA ATGAAAGTCA CAGGAATAAC TACTCAGGGA 
GTAAAATCTC TGCTTACCAG CATGTATGTG AAGGAGTTCC TCATCTCCAG CAGTCAAGAT 
GGCCATCACT GGACTCTCTT TTTTCAGAAT GGCAAAGTAA AGGTTTTTCA GGGAAATCAA 
GACTCCTTCA CACCTGTGGT GAACTCTCTA GACCCACCGT TACTGACTCG CTACCTTCGA 
ATTCACCCCC AGAGTTGGGT GCACCAGATT GCCCTGAGGA TGGAGGTTCT GGGCTGCGAG 
GCACAGGACC TCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_016944627.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016800116.1 Pan troglodytes coagulation factor VIII (F8), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPc91299
Clone ID Related Accession (Same CDS sequence)	XM_016944627.2 , XM_016944627.1
Accession Version	XM_016944627.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	coagulation factor VIII isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACCAT CCAAAGCTGG AATTTGGCGG GTGGAATGCC TTATTGGCGA GCATCTACAT 
GCTGGGATGA GCACACTTTT TCTGGTGTAC AGCAATAAGT GTCAGACTCC CCTGGGAATG 
GCTTCTGGAC ACATTAGAGA TTTTCAGATT ACAGCTTCAG GACAATATGG ACAGTGGGCC 
CCAAAGCTGG CCAGACTTCA TTATTCCGGA TCAATCAATG CCTGGAGCAC CAAGGAGCCC 
TTTTCTTGGA TCAAGGTGGA TCTGTTGGCA CCAATGATTA TTCACGGCAT CAAGACCCAG 
GGTGCCCGTC AGAAGTTCTC CAGCCTCTAC ATCTCTCAGT TTATCATCAT GTATAGTCTT 
GATGGGAAGA AGTGGCAGAC TTATCGAGGA AATTCCACTG GAACCTTAAT GGTCTTCTTT 
GGCAATGTGG ATTCATCTGG GATAAAACAC AATATTTTTA ACCCTCCAAT TATTGCTCGA 
TACATCCGTT TGCACCCAAC TCATTATAGC ATTCGCAGCA CTCTTCGCAT GGAGTTGATG 
GGCTGTGATT TAAATAGTTG CAGCATGCCA TTGGGAATGG AGAGTAAAGC AATATCAGAT 
GCACAGATTA CTGCTTCATC CTACTTTACC AATATGTTTG CCACCTGGTC TCCTTCAAAA 
GCTCGACTTC ACCTCCAAGG GAGGAGTAAT GCCTGGAGAC CTCAGGTGAA TAATCCAAAA 
GAGTGGCTGC AAGTGGACTT CCAGAAGACA ATGAAAGTCA CAGGAATAAC TACTCAGGGA 
GTAAAATCTC TGCTTACCAG CATGTATGTG AAGGAGTTCC TCATCTCCAG CAGTCAAGAT 
GGCCATCACT GGACTCTCTT TTTTCAGAAT GGCAAAGTAA AGGTTTTTCA GGGAAATCAA 
GACTCCTTCA CACCTGTGGT GAACTCTCTA GACCCACCGT TACTGACTCG CTACCTTCGA 
ATTCACCCCC AGAGTTGGGT GCACCAGATT GCCCTGAGGA TGGAGGTTCT GGGCTGCGAG 
GCACAGGACC TCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016800116.1
CDS	425..1462
Translation	MLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGHIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTSMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY

Target ORF information:

RefSeq Version	XM_016944627.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes coagulation factor VIII (F8), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016944627.1

ORF Insert Sequence:

ATGTTACCAT CCAAAGCTGG AATTTGGCGG GTGGAATGCC TTATTGGCGA GCATCTACAT 
GCTGGGATGA GCACACTTTT TCTGGTGTAC AGCAATAAGT GTCAGACTCC CCTGGGAATG 
GCTTCTGGAC ACATTAGAGA TTTTCAGATT ACAGCTTCAG GACAATATGG ACAGTGGGCC 
CCAAAGCTGG CCAGACTTCA TTATTCCGGA TCAATCAATG CCTGGAGCAC CAAGGAGCCC 
TTTTCTTGGA TCAAGGTGGA TCTGTTGGCA CCAATGATTA TTCACGGCAT CAAGACCCAG 
GGTGCCCGTC AGAAGTTCTC CAGCCTCTAC ATCTCTCAGT TTATCATCAT GTATAGTCTT 
GATGGGAAGA AGTGGCAGAC TTATCGAGGA AATTCCACTG GAACCTTAAT GGTCTTCTTT 
GGCAATGTGG ATTCATCTGG GATAAAACAC AATATTTTTA ACCCTCCAAT TATTGCTCGA 
TACATCCGTT TGCACCCAAC TCATTATAGC ATTCGCAGCA CTCTTCGCAT GGAGTTGATG 
GGCTGTGATT TAAATAGTTG CAGCATGCCA TTGGGAATGG AGAGTAAAGC AATATCAGAT 
GCACAGATTA CTGCTTCATC CTACTTTACC AATATGTTTG CCACCTGGTC TCCTTCAAAA 
GCTCGACTTC ACCTCCAAGG GAGGAGTAAT GCCTGGAGAC CTCAGGTGAA TAATCCAAAA 
GAGTGGCTGC AAGTGGACTT CCAGAAGACA ATGAAAGTCA CAGGAATAAC TACTCAGGGA 
GTAAAATCTC TGCTTACCAG CATGTATGTG AAGGAGTTCC TCATCTCCAG CAGTCAAGAT 
GGCCATCACT GGACTCTCTT TTTTCAGAAT GGCAAAGTAA AGGTTTTTCA GGGAAATCAA 
GACTCCTTCA CACCTGTGGT GAACTCTCTA GACCCACCGT TACTGACTCG CTACCTTCGA 
ATTCACCCCC AGAGTTGGGT GCACCAGATT GCCCTGAGGA TGGAGGTTCT GGGCTGCGAG 
GCACAGGACC TCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_016944626.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016800115.1 Pan troglodytes coagulation factor VIII (F8), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPc91298
Clone ID Related Accession (Same CDS sequence)	XM_016944626.1 , XM_016944626.2
Accession Version	XM_016944626.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	coagulation factor VIII isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC 
TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA 
GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG 
AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA 
TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA 
AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA 
TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA 
GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG 
CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA 
GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG 
GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA 
AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA 
AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC 
AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT 
CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT 
ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT 
AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG 
AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT 
CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT 
GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG 
AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC 
ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC 
ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT 
CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC 
AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA 
TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT 
GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT 
GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT 
ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT 
CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA 
CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC 
TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT 
CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT 
ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA 
ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC 
CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT 
CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG 
AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC 
ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT 
CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA 
GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC 
ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG 
GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA 
CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG 
GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016800115.1
CDS	427..5442
Translation	MVYEDTLTLFPFSGETVFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISAYLLSKNNAIEPRSFSQNSRHPSTRQKQFNATTIPENDIEKTDPWFAHRTPMPKVQNVSSSDLLMLLRQSPTPHGLSLSDLQEAKYETFSDDPSPGAIDSNNSLSEMTHFRPQFHHSGDMVFTPESGLQLRLNEKLGTTAATELKKLDFKVSSTSNNLISTIPSDNLAAGTDNTSSLGPPSMPVHYDSQLDTTLFGKKSSPLTESGRPLSLSEENNDSKLLESGLMNSQESSWGKNVSSTESGRLFKGKRAHGPALLTKDNALFKVSISLLKTNKTSNNSATNRKTHIDGPSLLIQSSPSVWQNILESDTEFKKVTPLIHDRMLMDKNATALRLNHMSNKTTSSKNMEMVRQKKEGPIPPDAENPDMSFFKMLFLPESASWIQRTHGKNSLNSGQGPSPKQLVSLGPEKSVEGQNFLSEKNKVVVGKGEFTKDVGLKEMVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIERKETLIQENVVLPQIHTVTGTKNFMKNLFLLSTRQNVEGSYEGAYAPVLQDFRSLNDSTNRTKKHTAHFSKKGEEENLAGLGNQTKQIVEKYACTTRISPNTSQQNFVTQRSKRALKQFRLPLEETELEKRIIVDDTSTQWSKNMKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSIPQANRSPLPIAEVSSFPSIRPIYLTRVLFQDNSSHLPAASYRKKDSGVQESSHFLQGAKKNNLSLAILTLEMTGDQREVGSLGTSATNSVTYKKVENTVLPKPGLPKTSGKVELLPKVHIYQKDLFPTETSNGSPGHLDLVEGSLLQETEGAIKWNEANRPGKIPFLRVATESSAKTPSKLLDPLAWDNHYGTQIPKEEWKSQEKSPEKTAFKKKDTILSLNACESNHAIAAINEGQNKPEIEVTWAKQGRTERLCSQNPPGLKRHQREITRTTLQSDQEEIDYDDTISVEMKKEDFDIYDEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSGSVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFDETKSWYFTENMERNCRAPCNIQMEDPTFKENYRFHAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMALYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGHIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTSMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY

Target ORF information:

RefSeq Version	XM_016944626.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes coagulation factor VIII (F8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016944626.1

ORF Insert Sequence:

ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC 
TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA 
GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG 
AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA 
TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA 
AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA 
TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA 
GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG 
CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA 
GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG 
GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA 
AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA 
AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC 
AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT 
CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT 
ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT 
AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG 
AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT 
CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT 
GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG 
AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC 
ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC 
ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT 
CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC 
AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA 
TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT 
GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT 
GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT 
ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT 
CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA 
CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC 
TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT 
CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT 
ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA 
ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC 
CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT 
CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG 
AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC 
ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT 
CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA 
GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC 
ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG 
GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA 
CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG 
GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_016944626.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016800115.1 Pan troglodytes coagulation factor VIII (F8), transcript variant X5, mRNA.

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CloneID	OPc91298
Clone ID Related Accession (Same CDS sequence)	XM_016944626.1 , XM_016944626.2
Accession Version	XM_016944626.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	coagulation factor VIII isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016944626.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC 
TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA 
GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG 
AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA 
TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA 
AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA 
TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA 
GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG 
CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA 
GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG 
GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA 
AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA 
AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC 
AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT 
CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT 
ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT 
AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG 
AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT 
CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT 
GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG 
AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC 
ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC 
ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT 
CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC 
AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA 
TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT 
GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT 
GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT 
ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT 
CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA 
CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC 
TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT 
CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT 
ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA 
ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC 
CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT 
CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG 
AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC 
ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT 
CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA 
GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC 
ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG 
GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA 
CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG 
GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016800115.1
CDS	419..5434
Translation	MVYEDTLTLFPFSGETVFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISAYLLSKNNAIEPRSFSQNSRHPSTRQKQFNATTIPENDIEKTDPWFAHRTPMPKVQNVSSSDLLMLLRQSPTPHGLSLSDLQEAKYETFSDDPSPGAIDSNNSLSEMTHFRPQFHHSGDMVFTPESGLQLRLNEKLGTTAATELKKLDFKVSSTSNNLISTIPSDNLAAGTDNTSSLGPPSMPVHYDSQLDTTLFGKKSSPLTESGRPLSLSEENNDSKLLESGLMNSQESSWGKNVSSTESGRLFKGKRAHGPALLTKDNALFKVSISLLKTNKTSNNSATNRKTHIDGPSLLIQSSPSVWQNILESDTEFKKVTPLIHDRMLMDKNATALRLNHMSNKTTSSKNMEMVRQKKEGPIPPDAENPDMSFFKMLFLPESASWIQRTHGKNSLNSGQGPSPKQLVSLGPEKSVEGQNFLSEKNKVVVGKGEFTKDVGLKEMVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIERKETLIQENVVLPQIHTVTGTKNFMKNLFLLSTRQNVEGSYEGAYAPVLQDFRSLNDSTNRTKKHTAHFSKKGEEENLAGLGNQTKQIVEKYACTTRISPNTSQQNFVTQRSKRALKQFRLPLEETELEKRIIVDDTSTQWSKNMKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSIPQANRSPLPIAEVSSFPSIRPIYLTRVLFQDNSSHLPAASYRKKDSGVQESSHFLQGAKKNNLSLAILTLEMTGDQREVGSLGTSATNSVTYKKVENTVLPKPGLPKTSGKVELLPKVHIYQKDLFPTETSNGSPGHLDLVEGSLLQETEGAIKWNEANRPGKIPFLRVATESSAKTPSKLLDPLAWDNHYGTQIPKEEWKSQEKSPEKTAFKKKDTILSLNACESNHAIAAINEGQNKPEIEVTWAKQGRTERLCSQNPPGLKRHQREITRTTLQSDQEEIDYDDTISVEMKKEDFDIYDEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSGSVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFDETKSWYFTENMERNCRAPCNIQMEDPTFKENYRFHAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMALYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGHIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTSMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY

Target ORF information:

RefSeq Version	XM_016944626.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes coagulation factor VIII (F8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016944626.2

ORF Insert Sequence:

ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC 
TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA 
GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG 
AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA 
TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA 
AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA 
TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA 
GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG 
CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA 
GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG 
GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA 
AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA 
AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC 
AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT 
CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT 
ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT 
AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG 
AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT 
CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT 
GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG 
AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC 
ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC 
ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT 
CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC 
AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA 
TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT 
GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT 
GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT 
ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT 
CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA 
CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC 
TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT 
CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT 
ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA 
ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC 
CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT 
CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG 
AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC 
ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT 
CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA 
GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC 
ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG 
GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA 
CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG 
GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_016944624.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016800113.1 Pan troglodytes coagulation factor VIII (F8), transcript variant X2, mRNA.

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CloneID	OPc91296
Clone ID Related Accession (Same CDS sequence)	XM_016944624.1
Accession Version	XM_016944624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	coagulation factor VIII isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCGAG ATGCGCGTGC GCAGAAAGCA TCCCGGGGAT TTCCTCCTAG AGTGCCAAAA 
TCTTTTCCAT TCAACACCTC AGTCGTGTAC AAAAAGACTG TGTTTGTAGA ATTCACGGAT 
CACCTTTTCA ACATCGCTAA GCCAAGGCCA CCCTGGATGG GTCTGCTAGG TCCTACCATC 
CAGGCTGAGG TTTATGATAC AGTGGTCATT ACACTTAAGA ACATGGCTTC CCATCCTGTC 
AGTCTTCATG CTGTTGGTGT ATCCTACTGG AAAGCTTCTG AGGGAGCTGA ATATGATGAT 
CAGACCAGTC AAAGGGAGAA AGAAGATGAT AAAGTCTTTC CTGGTGGAAG CCATACATAT 
GTCTGGCAGG TCCTGAAAGA GAATGGTCCA ATGGCCTCTG ACCCACTGTG CCTTACCTAC 
TCATATCTTT CTCATGTGGA CCTGGTCAAA GACTTGAATT CAGGCCTCAT TGGAGCCCTA 
CTAGTATGTA GAGAAGGGAG TCTGGCCAAG GAAAAGACAC AGACCTTGCA CAAATTTATA 
CTACTTTTTG CTGTATTTGA TGAAGGGAAA AGTTGGCACT CAGAAACAAA GAACTCCTTG 
ATGCAGGATA GGGATGCTGC ATCTGCTCGG GCCTGGCCTA AAATGCACAC AGTCAATGGT 
TATGTAAACA GGTCTCTGCC AGGTCTGATT GGATGCCACA GGAAATCAGT CTATTGGCAT 
GTGATTGGAA TGGGCACCAC TCCTGAAGTG CACTCAATAT TCCTCGAAGG TCACACATTT 
CTTGTGAGGA ACCATCGCCA GGCGTCCTTG GAAATATCGC CAATAACTTT CCTTACTGCT 
CAAACACTCT TGATGGACCT TGGACAGTTT CTACTGTTTT GTCATATCTC TTCCCACCAA 
CATGATGGCA TGGAAGCTTA TGTCAAAGTA GACAGCTGCC CAGAGGAACC CCAACTACGA 
ATGAAAAATA ATGAAGAAGC GGAAGACTAT GATGATGATC TTACTGATTC TGAAATGGAT 
GTGGTCAGGT TTGATGATGA CAACTCTCCT TCCTTTATCC AAATTCGCTC AGTTGCCAAG 
AAGCATCCTA AAACTTGGGT ACATTACATT GCTGCTGAAG AGGAGGACTG GGACTATGCT 
CCCTTAGTCC TCGCCCCCGA TGACAGAAGT TATAAAAGTC AATATTTGAA CAATGGCCCT 
CAGCGGATTG GTAGGAAGTA CAAAAAAGTC CGATTTATGG CATACACAGA TGAAACCTTT 
AAGACTCGTG AAGCTATTCA GCATGAATCA GGAATCTTGG GACCTTTACT TTATGGGGAA 
GTTGGAGACA CACTGTTGAT TATATTTAAG AATCAAGCAA GCAGACCATA TAACATCTAC 
CCTCACGGAA TCACTGATGT CCGTCCTTTG TATTCAAGGA GATTACCAAA AGGTGTAAAA 
CATTTGAAGG ATTTTCCAAT TCTGCCAGGA GAAATATTCA AATATAAATG GACAGTGACT 
GTAGAAGATG GGCCAACTAA ATCAGATCCT CGGTGCCTGA CCCGCTATTA CTCTAGTTTC 
GTTAATATGG AGAGAGATCT AGCTTCAGGA CTCATTGGCC CTCTCCTCAT CTGCTACAAA 
GAATCTGTAG ATCAAAGAGG AAACCAGATA ATGTCAGACA AGAGGAATGT CATCCTGTTT 
TCTGTATTTG ATGAGAACCG AAGCTGGTAC CTCACAGAGA ATATACAACG CTTTCTCCCC 
AATCCAGCTG GAGTGCAGCT TGAGGATCCA GAGTTCCAAG CCTCCAACAT CATGCACAGC 
ATCAATGGCT ATGTTTTTGA TAGTTTGCAG TTGTCAGTTT GTTTGCATGA GGTGGCATAC 
TGGTACATTC TAAGCATTGG AGCACAGACT GACTTCCTTT CTGTCTTCTT CTCTGGATAT 
ACCTTCAAAC ACAAAATGGT CTATGAAGAT ACACTCACCC TATTCCCATT CTCAGGAGAA 
ACTGTCTTCA TGTCGATGGA AAACCCAGGC CTATGGATTC TGGGGTGCCA CAACTCAGAC 
TTTCGGAACA GAGGCATGAC CGCCTTACTG AAGGTTTCTA GTTGTGACAA GAACACTGGT 
GATTATTACG AGGACAGTTA TGAAGATATT TCAGCATACT TGCTGAGTAA AAACAATGCC 
ATTGAACCAA GAAGCTTCTC CCAGAATTCA AGACACCCTA GCACTAGGCA AAAGCAATTT 
AATGCCACCA CAATTCCAGA AAATGACATA GAGAAGACTG ACCCTTGGTT TGCACACAGA 
ACACCTATGC CTAAAGTACA AAATGTCTCC TCTAGTGATT TGTTGATGCT CTTGCGACAG 
AGTCCTACTC CACATGGGCT ATCCTTATCT GATCTCCAAG AAGCCAAATA TGAGACTTTT 
TCTGATGATC CATCACCTGG AGCAATAGAC AGTAATAACA GCCTATCTGA AATGACACAC 
TTCAGGCCAC AGTTCCATCA CAGTGGGGAC ATGGTATTTA CCCCTGAGTC AGGCCTCCAA 
TTGAGATTAA ATGAGAAACT GGGGACAACT GCAGCAACAG AGTTGAAGAA ACTTGATTTC 
AAAGTTTCTA GTACATCAAA TAATCTGATT TCAACAATTC CATCAGACAA TTTGGCAGCA 
GGTACTGATA ATACAAGTTC CTTAGGACCC CCAAGTATGC CAGTTCATTA TGATAGTCAA 
TTAGATACCA CTCTATTTGG CAAAAAGTCA TCTCCCCTTA CTGAGTCTGG TAGACCTCTG 
AGCTTGAGTG AAGAAAATAA TGATTCAAAG TTGTTAGAAT CAGGTTTAAT GAATAGCCAA 
GAAAGTTCAT GGGGAAAAAA TGTATCGTCA ACAGAGAGTG GTAGGTTATT TAAAGGGAAA 
AGAGCTCATG GACCTGCTTT GTTGACTAAA GATAATGCCT TATTCAAAGT TAGCATCTCT 
TTGTTAAAGA CAAACAAAAC TTCCAATAAT TCAGCAACTA ATAGAAAGAC TCACATTGAT 
GGCCCATCAT TATTAATTCA GAGTAGTCCA TCAGTCTGGC AAAATATATT AGAAAGTGAC 
ACTGAGTTTA AAAAAGTGAC ACCTTTGATT CATGACAGAA TGCTTATGGA CAAAAATGCT 
ACAGCTTTGA GGCTAAATCA TATGTCAAAT AAAACTACTT CATCAAAAAA CATGGAAATG 
GTCCGACAGA AAAAAGAGGG CCCCATTCCA CCAGATGCAG AAAATCCAGA TATGTCATTC 
TTTAAGATGC TATTCTTGCC AGAATCAGCA AGTTGGATAC AAAGGACTCA TGGAAAGAAC 
TCTCTGAACT CTGGGCAAGG CCCCAGTCCA AAGCAATTAG TATCCTTAGG ACCAGAAAAA 
TCTGTGGAAG GTCAGAATTT CTTGTCTGAG AAAAACAAAG TGGTAGTAGG AAAGGGTGAA 
TTTACAAAGG ACGTAGGACT CAAAGAGATG GTTTTTCCAA GCAGCAGAAA CCTATTTCTT 
ACTAACTTGG ATAATTTACA TGAAAATAAT ACACACAATC AAGAAAAAAA AATTCAGGAA 
GAAATAGAAA GGAAGGAAAC ATTAATCCAA GAGAATGTAG TTTTGCCTCA GATACATACA 
GTGACTGGCA CTAAGAATTT CATGAAGAAC CTTTTCTTAC TGAGCACTAG GCAAAATGTA 
GAAGGTTCAT ATGAGGGGGC ATATGCTCCA GTACTTCAAG ATTTTAGGTC ATTAAATGAT 
TCAACAAATA GAACAAAGAA ACACACGGCT CATTTCTCAA AAAAAGGGGA AGAAGAAAAC 
TTGGCAGGCT TGGGAAATCA AACCAAGCAA ATTGTAGAGA AATATGCATG CACCACAAGG 
ATATCTCCTA ATACAAGCCA GCAGAATTTT GTCACGCAAC GTAGTAAGAG AGCTTTGAAA 
CAATTCAGAC TCCCACTAGA AGAAACAGAA CTTGAAAAAA GGATAATTGT GGATGACACC 
TCAACCCAGT GGTCCAAAAA CATGAAACAT TTGACCCCGA GCACCCTCAC ACAGATAGAC 
TACAATGAGA AGGAGAAAGG GGCCATTACT CAGTCTCCCT TATCAGATTG CCTTACGAGG 
AGTCATAGCA TCCCTCAAGC AAATAGATCT CCATTACCCA TTGCAGAGGT ATCATCATTT 
CCATCTATTA GACCTATATA TCTGACCAGG GTCCTATTCC AAGACAACTC TTCTCATCTT 
CCAGCAGCAT CTTATAGAAA GAAAGATTCT GGGGTCCAAG AAAGCAGTCA TTTCTTACAA 
GGAGCCAAAA AAAATAACCT TTCTTTAGCC ATTCTAACCT TGGAGATGAC TGGTGATCAA 
AGAGAGGTTG GCTCCCTGGG GACAAGTGCC ACAAATTCAG TCACTTACAA GAAAGTTGAG 
AACACTGTTC TCCCGAAACC AGGCTTGCCC AAAACATCTG GCAAAGTTGA ATTGCTTCCA 
AAAGTTCACA TTTATCAGAA GGACCTATTC CCTACGGAAA CTAGCAATGG GTCTCCTGGC 
CATCTGGATC TCGTGGAAGG GAGCCTTCTT CAGGAAACAG AGGGAGCGAT TAAGTGGAAT 
GAAGCAAACA GACCTGGAAA AATTCCCTTT CTGAGAGTAG CAACAGAAAG CTCTGCAAAG 
ACTCCCTCCA AGCTATTGGA TCCTCTTGCT TGGGATAACC ACTATGGTAC TCAGATACCA 
AAAGAAGAGT GGAAATCCCA AGAGAAGTCA CCAGAAAAAA CAGCTTTTAA GAAAAAGGAT 
ACCATTTTGT CCCTGAACGC TTGTGAAAGC AATCATGCAA TAGCAGCAAT AAATGAGGGA 
CAAAATAAGC CCGAAATAGA AGTCACCTGG GCAAAGCAAG GTAGGACTGA AAGGCTGTGC 
TCTCAAAACC CACCAGGCTT GAAACGCCAT CAACGGGAAA TAACTCGTAC TACTCTTCAG 
TCAGATCAAG AGGAAATTGA CTATGATGAT ACCATCTCAG TTGAAATGAA GAAGGAAGAT 
TTTGACATTT ATGATGAGGA TGAAAATCAG AGCCCCCGCA GCTTTCAAAA GAAAACACGA 
CACTATTTTA TTGCTGCAGT GGAGAGGCTC TGGGATTATG GGATGAGTAG CTCCCCACAT 
GTTCTAAGAA ACAGGGCTCA GAGTGGCAGT GTCCCTCAGT TCAAGAAAGT TGTTTTCCAG 
GAATTTACTG ATGGCTCCTT TACTCAGCCC TTATACCGTG GAGAACTAAA TGAACATTTG 
GGACTCCTGG GGCCATATAT AAGAGCAGAA GTTGAAGATA ATATCATGGT AACTTTCAAA 
AATCAGGCCT CTCGTCCCTA TTCCTTCTAT TCTAGCCTTA TTTCTTATGA GGAAGATCAG 
AGGCAAGGAG CAGAACCTAG GAAAAACTTT GTCAAGCCTA ATGAAACCAA AACTTACTTT 
TGGAAAGTGC AACATCATAT GGCGCCCACT AAAGATGAGT TTGACTGCAA AGCCTGGGCT 
TATTTCTCTG ATGTTGACCT GGAAAAAGAT GTGCACTCAG GCCTGATTGG ACCCCTTCTG 
GTCTGCCACA CTAACACACT GAACCCTGCT CATGGGAGAC AAGTGACAGT ACAGGAATTT 
GCTCTGTTTT TCACCATCTT TGATGAGACC AAAAGCTGGT ACTTCACTGA AAACATGGAA 
AGAAACTGCA GGGCTCCCTG CAATATCCAG ATGGAAGATC CCACTTTTAA AGAGAATTAT 
CGCTTCCATG CAATCAATGG CTACATAATG GATACACTAC CTGGCTTAGT AATGGCTCAG 
GATCAAAGGA TTCGATGGTA TCTGCTCAGC ATGGGCAGCA ATGAAAACAT CCATTCTATT 
CATTTCAGTG GACATGTGTT CACTGTACGA AAAAAAGAGG AGTATAAAAT GGCACTGTAC 
AATCTCTATC CAGGTGTTTT TGAGACAGTG GAAATGTTAC CATCCAAAGC TGGAATTTGG 
CGGGTGGAAT GCCTTATTGG CGAGCATCTA CATGCTGGGA TGAGCACACT TTTTCTGGTG 
TACAGCAATA AGTGTCAGAC TCCCCTGGGA ATGGCTTCTG GACACATTAG AGATTTTCAG 
ATTACAGCTT CAGGACAATA TGGACAGTGG GCCCCAAAGC TGGCCAGACT TCATTATTCC 
GGATCAATCA ATGCCTGGAG CACCAAGGAG CCCTTTTCTT GGATCAAGGT GGATCTGTTG 
GCACCAATGA TTATTCACGG CATCAAGACC CAGGGTGCCC GTCAGAAGTT CTCCAGCCTC 
TACATCTCTC AGTTTATCAT CATGTATAGT CTTGATGGGA AGAAGTGGCA GACTTATCGA 
GGAAATTCCA CTGGAACCTT AATGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA 
CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT 
AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG 
CCATTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTT 
ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTCACCTCCA AGGGAGGAGT 
AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG 
ACAATGAAAG TCACAGGAAT AACTACTCAG GGAGTAAAAT CTCTGCTTAC CAGCATGTAT 
GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC ACTGGACTCT CTTTTTTCAG 
AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT 
CTAGACCCAC CGTTACTGAC TCGCTACCTT CGAATTCACC CCCAGAGTTG GGTGCACCAG 
ATTGCCCTGA GGATGGAGGT TCTGGGCTGC GAGGCACAGG ACCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016800113.1
CDS	118..7068
Translation	MHRDARAQKASRGFPPRVPKSFPFNTSVVYKKTVFVEFTDHLFNIAKPRPPWMGLLGPTIQAEVYDTVVITLKNMASHPVSLHAVGVSYWKASEGAEYDDQTSQREKEDDKVFPGGSHTYVWQVLKENGPMASDPLCLTYSYLSHVDLVKDLNSGLIGALLVCREGSLAKEKTQTLHKFILLFAVFDEGKSWHSETKNSLMQDRDAASARAWPKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTLLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKNNEEAEDYDDDLTDSEMDVVRFDDDNSPSFIQIRSVAKKHPKTWVHYIAAEEEDWDYAPLVLAPDDRSYKSQYLNNGPQRIGRKYKKVRFMAYTDETFKTREAIQHESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVRPLYSRRLPKGVKHLKDFPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFVNMERDLASGLIGPLLICYKESVDQRGNQIMSDKRNVILFSVFDENRSWYLTENIQRFLPNPAGVQLEDPEFQASNIMHSINGYVFDSLQLSVCLHEVAYWYILSIGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISAYLLSKNNAIEPRSFSQNSRHPSTRQKQFNATTIPENDIEKTDPWFAHRTPMPKVQNVSSSDLLMLLRQSPTPHGLSLSDLQEAKYETFSDDPSPGAIDSNNSLSEMTHFRPQFHHSGDMVFTPESGLQLRLNEKLGTTAATELKKLDFKVSSTSNNLISTIPSDNLAAGTDNTSSLGPPSMPVHYDSQLDTTLFGKKSSPLTESGRPLSLSEENNDSKLLESGLMNSQESSWGKNVSSTESGRLFKGKRAHGPALLTKDNALFKVSISLLKTNKTSNNSATNRKTHIDGPSLLIQSSPSVWQNILESDTEFKKVTPLIHDRMLMDKNATALRLNHMSNKTTSSKNMEMVRQKKEGPIPPDAENPDMSFFKMLFLPESASWIQRTHGKNSLNSGQGPSPKQLVSLGPEKSVEGQNFLSEKNKVVVGKGEFTKDVGLKEMVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIERKETLIQENVVLPQIHTVTGTKNFMKNLFLLSTRQNVEGSYEGAYAPVLQDFRSLNDSTNRTKKHTAHFSKKGEEENLAGLGNQTKQIVEKYACTTRISPNTSQQNFVTQRSKRALKQFRLPLEETELEKRIIVDDTSTQWSKNMKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSIPQANRSPLPIAEVSSFPSIRPIYLTRVLFQDNSSHLPAASYRKKDSGVQESSHFLQGAKKNNLSLAILTLEMTGDQREVGSLGTSATNSVTYKKVENTVLPKPGLPKTSGKVELLPKVHIYQKDLFPTETSNGSPGHLDLVEGSLLQETEGAIKWNEANRPGKIPFLRVATESSAKTPSKLLDPLAWDNHYGTQIPKEEWKSQEKSPEKTAFKKKDTILSLNACESNHAIAAINEGQNKPEIEVTWAKQGRTERLCSQNPPGLKRHQREITRTTLQSDQEEIDYDDTISVEMKKEDFDIYDEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSGSVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFDETKSWYFTENMERNCRAPCNIQMEDPTFKENYRFHAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMALYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGHIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTSMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY

Target ORF information:

RefSeq Version	XM_016944624.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes coagulation factor VIII (F8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016944624.1

ORF Insert Sequence:

ATGCACCGAG ATGCGCGTGC GCAGAAAGCA TCCCGGGGAT TTCCTCCTAG AGTGCCAAAA 
TCTTTTCCAT TCAACACCTC AGTCGTGTAC AAAAAGACTG TGTTTGTAGA ATTCACGGAT 
CACCTTTTCA ACATCGCTAA GCCAAGGCCA CCCTGGATGG GTCTGCTAGG TCCTACCATC 
CAGGCTGAGG TTTATGATAC AGTGGTCATT ACACTTAAGA ACATGGCTTC CCATCCTGTC 
AGTCTTCATG CTGTTGGTGT ATCCTACTGG AAAGCTTCTG AGGGAGCTGA ATATGATGAT 
CAGACCAGTC AAAGGGAGAA AGAAGATGAT AAAGTCTTTC CTGGTGGAAG CCATACATAT 
GTCTGGCAGG TCCTGAAAGA GAATGGTCCA ATGGCCTCTG ACCCACTGTG CCTTACCTAC 
TCATATCTTT CTCATGTGGA CCTGGTCAAA GACTTGAATT CAGGCCTCAT TGGAGCCCTA 
CTAGTATGTA GAGAAGGGAG TCTGGCCAAG GAAAAGACAC AGACCTTGCA CAAATTTATA 
CTACTTTTTG CTGTATTTGA TGAAGGGAAA AGTTGGCACT CAGAAACAAA GAACTCCTTG 
ATGCAGGATA GGGATGCTGC ATCTGCTCGG GCCTGGCCTA AAATGCACAC AGTCAATGGT 
TATGTAAACA GGTCTCTGCC AGGTCTGATT GGATGCCACA GGAAATCAGT CTATTGGCAT 
GTGATTGGAA TGGGCACCAC TCCTGAAGTG CACTCAATAT TCCTCGAAGG TCACACATTT 
CTTGTGAGGA ACCATCGCCA GGCGTCCTTG GAAATATCGC CAATAACTTT CCTTACTGCT 
CAAACACTCT TGATGGACCT TGGACAGTTT CTACTGTTTT GTCATATCTC TTCCCACCAA 
CATGATGGCA TGGAAGCTTA TGTCAAAGTA GACAGCTGCC CAGAGGAACC CCAACTACGA 
ATGAAAAATA ATGAAGAAGC GGAAGACTAT GATGATGATC TTACTGATTC TGAAATGGAT 
GTGGTCAGGT TTGATGATGA CAACTCTCCT TCCTTTATCC AAATTCGCTC AGTTGCCAAG 
AAGCATCCTA AAACTTGGGT ACATTACATT GCTGCTGAAG AGGAGGACTG GGACTATGCT 
CCCTTAGTCC TCGCCCCCGA TGACAGAAGT TATAAAAGTC AATATTTGAA CAATGGCCCT 
CAGCGGATTG GTAGGAAGTA CAAAAAAGTC CGATTTATGG CATACACAGA TGAAACCTTT 
AAGACTCGTG AAGCTATTCA GCATGAATCA GGAATCTTGG GACCTTTACT TTATGGGGAA 
GTTGGAGACA CACTGTTGAT TATATTTAAG AATCAAGCAA GCAGACCATA TAACATCTAC 
CCTCACGGAA TCACTGATGT CCGTCCTTTG TATTCAAGGA GATTACCAAA AGGTGTAAAA 
CATTTGAAGG ATTTTCCAAT TCTGCCAGGA GAAATATTCA AATATAAATG GACAGTGACT 
GTAGAAGATG GGCCAACTAA ATCAGATCCT CGGTGCCTGA CCCGCTATTA CTCTAGTTTC 
GTTAATATGG AGAGAGATCT AGCTTCAGGA CTCATTGGCC CTCTCCTCAT CTGCTACAAA 
GAATCTGTAG ATCAAAGAGG AAACCAGATA ATGTCAGACA AGAGGAATGT CATCCTGTTT 
TCTGTATTTG ATGAGAACCG AAGCTGGTAC CTCACAGAGA ATATACAACG CTTTCTCCCC 
AATCCAGCTG GAGTGCAGCT TGAGGATCCA GAGTTCCAAG CCTCCAACAT CATGCACAGC 
ATCAATGGCT ATGTTTTTGA TAGTTTGCAG TTGTCAGTTT GTTTGCATGA GGTGGCATAC 
TGGTACATTC TAAGCATTGG AGCACAGACT GACTTCCTTT CTGTCTTCTT CTCTGGATAT 
ACCTTCAAAC ACAAAATGGT CTATGAAGAT ACACTCACCC TATTCCCATT CTCAGGAGAA 
ACTGTCTTCA TGTCGATGGA AAACCCAGGC CTATGGATTC TGGGGTGCCA CAACTCAGAC 
TTTCGGAACA GAGGCATGAC CGCCTTACTG AAGGTTTCTA GTTGTGACAA GAACACTGGT 
GATTATTACG AGGACAGTTA TGAAGATATT TCAGCATACT TGCTGAGTAA AAACAATGCC 
ATTGAACCAA GAAGCTTCTC CCAGAATTCA AGACACCCTA GCACTAGGCA AAAGCAATTT 
AATGCCACCA CAATTCCAGA AAATGACATA GAGAAGACTG ACCCTTGGTT TGCACACAGA 
ACACCTATGC CTAAAGTACA AAATGTCTCC TCTAGTGATT TGTTGATGCT CTTGCGACAG 
AGTCCTACTC CACATGGGCT ATCCTTATCT GATCTCCAAG AAGCCAAATA TGAGACTTTT 
TCTGATGATC CATCACCTGG AGCAATAGAC AGTAATAACA GCCTATCTGA AATGACACAC 
TTCAGGCCAC AGTTCCATCA CAGTGGGGAC ATGGTATTTA CCCCTGAGTC AGGCCTCCAA 
TTGAGATTAA ATGAGAAACT GGGGACAACT GCAGCAACAG AGTTGAAGAA ACTTGATTTC 
AAAGTTTCTA GTACATCAAA TAATCTGATT TCAACAATTC CATCAGACAA TTTGGCAGCA 
GGTACTGATA ATACAAGTTC CTTAGGACCC CCAAGTATGC CAGTTCATTA TGATAGTCAA 
TTAGATACCA CTCTATTTGG CAAAAAGTCA TCTCCCCTTA CTGAGTCTGG TAGACCTCTG 
AGCTTGAGTG AAGAAAATAA TGATTCAAAG TTGTTAGAAT CAGGTTTAAT GAATAGCCAA 
GAAAGTTCAT GGGGAAAAAA TGTATCGTCA ACAGAGAGTG GTAGGTTATT TAAAGGGAAA 
AGAGCTCATG GACCTGCTTT GTTGACTAAA GATAATGCCT TATTCAAAGT TAGCATCTCT 
TTGTTAAAGA CAAACAAAAC TTCCAATAAT TCAGCAACTA ATAGAAAGAC TCACATTGAT 
GGCCCATCAT TATTAATTCA GAGTAGTCCA TCAGTCTGGC AAAATATATT AGAAAGTGAC 
ACTGAGTTTA AAAAAGTGAC ACCTTTGATT CATGACAGAA TGCTTATGGA CAAAAATGCT 
ACAGCTTTGA GGCTAAATCA TATGTCAAAT AAAACTACTT CATCAAAAAA CATGGAAATG 
GTCCGACAGA AAAAAGAGGG CCCCATTCCA CCAGATGCAG AAAATCCAGA TATGTCATTC 
TTTAAGATGC TATTCTTGCC AGAATCAGCA AGTTGGATAC AAAGGACTCA TGGAAAGAAC 
TCTCTGAACT CTGGGCAAGG CCCCAGTCCA AAGCAATTAG TATCCTTAGG ACCAGAAAAA 
TCTGTGGAAG GTCAGAATTT CTTGTCTGAG AAAAACAAAG TGGTAGTAGG AAAGGGTGAA 
TTTACAAAGG ACGTAGGACT CAAAGAGATG GTTTTTCCAA GCAGCAGAAA CCTATTTCTT 
ACTAACTTGG ATAATTTACA TGAAAATAAT ACACACAATC AAGAAAAAAA AATTCAGGAA 
GAAATAGAAA GGAAGGAAAC ATTAATCCAA GAGAATGTAG TTTTGCCTCA GATACATACA 
GTGACTGGCA CTAAGAATTT CATGAAGAAC CTTTTCTTAC TGAGCACTAG GCAAAATGTA 
GAAGGTTCAT ATGAGGGGGC ATATGCTCCA GTACTTCAAG ATTTTAGGTC ATTAAATGAT 
TCAACAAATA GAACAAAGAA ACACACGGCT CATTTCTCAA AAAAAGGGGA AGAAGAAAAC 
TTGGCAGGCT TGGGAAATCA AACCAAGCAA ATTGTAGAGA AATATGCATG CACCACAAGG 
ATATCTCCTA ATACAAGCCA GCAGAATTTT GTCACGCAAC GTAGTAAGAG AGCTTTGAAA 
CAATTCAGAC TCCCACTAGA AGAAACAGAA CTTGAAAAAA GGATAATTGT GGATGACACC 
TCAACCCAGT GGTCCAAAAA CATGAAACAT TTGACCCCGA GCACCCTCAC ACAGATAGAC 
TACAATGAGA AGGAGAAAGG GGCCATTACT CAGTCTCCCT TATCAGATTG CCTTACGAGG 
AGTCATAGCA TCCCTCAAGC AAATAGATCT CCATTACCCA TTGCAGAGGT ATCATCATTT 
CCATCTATTA GACCTATATA TCTGACCAGG GTCCTATTCC AAGACAACTC TTCTCATCTT 
CCAGCAGCAT CTTATAGAAA GAAAGATTCT GGGGTCCAAG AAAGCAGTCA TTTCTTACAA 
GGAGCCAAAA AAAATAACCT TTCTTTAGCC ATTCTAACCT TGGAGATGAC TGGTGATCAA 
AGAGAGGTTG GCTCCCTGGG GACAAGTGCC ACAAATTCAG TCACTTACAA GAAAGTTGAG 
AACACTGTTC TCCCGAAACC AGGCTTGCCC AAAACATCTG GCAAAGTTGA ATTGCTTCCA 
AAAGTTCACA TTTATCAGAA GGACCTATTC CCTACGGAAA CTAGCAATGG GTCTCCTGGC 
CATCTGGATC TCGTGGAAGG GAGCCTTCTT CAGGAAACAG AGGGAGCGAT TAAGTGGAAT 
GAAGCAAACA GACCTGGAAA AATTCCCTTT CTGAGAGTAG CAACAGAAAG CTCTGCAAAG 
ACTCCCTCCA AGCTATTGGA TCCTCTTGCT TGGGATAACC ACTATGGTAC TCAGATACCA 
AAAGAAGAGT GGAAATCCCA AGAGAAGTCA CCAGAAAAAA CAGCTTTTAA GAAAAAGGAT 
ACCATTTTGT CCCTGAACGC TTGTGAAAGC AATCATGCAA TAGCAGCAAT AAATGAGGGA 
CAAAATAAGC CCGAAATAGA AGTCACCTGG GCAAAGCAAG GTAGGACTGA AAGGCTGTGC 
TCTCAAAACC CACCAGGCTT GAAACGCCAT CAACGGGAAA TAACTCGTAC TACTCTTCAG 
TCAGATCAAG AGGAAATTGA CTATGATGAT ACCATCTCAG TTGAAATGAA GAAGGAAGAT 
TTTGACATTT ATGATGAGGA TGAAAATCAG AGCCCCCGCA GCTTTCAAAA GAAAACACGA 
CACTATTTTA TTGCTGCAGT GGAGAGGCTC TGGGATTATG GGATGAGTAG CTCCCCACAT 
GTTCTAAGAA ACAGGGCTCA GAGTGGCAGT GTCCCTCAGT TCAAGAAAGT TGTTTTCCAG 
GAATTTACTG ATGGCTCCTT TACTCAGCCC TTATACCGTG GAGAACTAAA TGAACATTTG 
GGACTCCTGG GGCCATATAT AAGAGCAGAA GTTGAAGATA ATATCATGGT AACTTTCAAA 
AATCAGGCCT CTCGTCCCTA TTCCTTCTAT TCTAGCCTTA TTTCTTATGA GGAAGATCAG 
AGGCAAGGAG CAGAACCTAG GAAAAACTTT GTCAAGCCTA ATGAAACCAA AACTTACTTT 
TGGAAAGTGC AACATCATAT GGCGCCCACT AAAGATGAGT TTGACTGCAA AGCCTGGGCT 
TATTTCTCTG ATGTTGACCT GGAAAAAGAT GTGCACTCAG GCCTGATTGG ACCCCTTCTG 
GTCTGCCACA CTAACACACT GAACCCTGCT CATGGGAGAC AAGTGACAGT ACAGGAATTT 
GCTCTGTTTT TCACCATCTT TGATGAGACC AAAAGCTGGT ACTTCACTGA AAACATGGAA 
AGAAACTGCA GGGCTCCCTG CAATATCCAG ATGGAAGATC CCACTTTTAA AGAGAATTAT 
CGCTTCCATG CAATCAATGG CTACATAATG GATACACTAC CTGGCTTAGT AATGGCTCAG 
GATCAAAGGA TTCGATGGTA TCTGCTCAGC ATGGGCAGCA ATGAAAACAT CCATTCTATT 
CATTTCAGTG GACATGTGTT CACTGTACGA AAAAAAGAGG AGTATAAAAT GGCACTGTAC 
AATCTCTATC CAGGTGTTTT TGAGACAGTG GAAATGTTAC CATCCAAAGC TGGAATTTGG 
CGGGTGGAAT GCCTTATTGG CGAGCATCTA CATGCTGGGA TGAGCACACT TTTTCTGGTG 
TACAGCAATA AGTGTCAGAC TCCCCTGGGA ATGGCTTCTG GACACATTAG AGATTTTCAG 
ATTACAGCTT CAGGACAATA TGGACAGTGG GCCCCAAAGC TGGCCAGACT TCATTATTCC 
GGATCAATCA ATGCCTGGAG CACCAAGGAG CCCTTTTCTT GGATCAAGGT GGATCTGTTG 
GCACCAATGA TTATTCACGG CATCAAGACC CAGGGTGCCC GTCAGAAGTT CTCCAGCCTC 
TACATCTCTC AGTTTATCAT CATGTATAGT CTTGATGGGA AGAAGTGGCA GACTTATCGA 
GGAAATTCCA CTGGAACCTT AATGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA 
CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT 
AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG 
CCATTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTT 
ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTCACCTCCA AGGGAGGAGT 
AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG 
ACAATGAAAG TCACAGGAAT AACTACTCAG GGAGTAAAAT CTCTGCTTAC CAGCATGTAT 
GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC ACTGGACTCT CTTTTTTCAG 
AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT 
CTAGACCCAC CGTTACTGAC TCGCTACCTT CGAATTCACC CCCAGAGTTG GGTGCACCAG 
ATTGCCCTGA GGATGGAGGT TCTGGGCTGC GAGGCACAGG ACCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_016944625.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016800114.1 Pan troglodytes coagulation factor VIII (F8), transcript variant X3, mRNA.

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CloneID	OPc91297
Clone ID Related Accession (Same CDS sequence)	XM_016944625.1 , XM_016944625.2
Accession Version	XM_016944625.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	coagulation factor VIII isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCC ATCCTGTCAG TCTTCATGCT GTTGGTGTAT CCTACTGGAA AGCTTCTGAG 
GGAGCTGAAT ATGATGATCA GACCAGTCAA AGGGAGAAAG AAGATGATAA AGTCTTTCCT 
GGTGGAAGCC ATACATATGT CTGGCAGGTC CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC 
CCACTGTGCC TTACCTACTC ATATCTTTCT CATGTGGACC TGGTCAAAGA CTTGAATTCA 
GGCCTCATTG GAGCCCTACT AGTATGTAGA GAAGGGAGTC TGGCCAAGGA AAAGACACAG 
ACCTTGCACA AATTTATACT ACTTTTTGCT GTATTTGATG AAGGGAAAAG TTGGCACTCA 
GAAACAAAGA ACTCCTTGAT GCAGGATAGG GATGCTGCAT CTGCTCGGGC CTGGCCTAAA 
ATGCACACAG TCAATGGTTA TGTAAACAGG TCTCTGCCAG GTCTGATTGG ATGCCACAGG 
AAATCAGTCT ATTGGCATGT GATTGGAATG GGCACCACTC CTGAAGTGCA CTCAATATTC 
CTCGAAGGTC ACACATTTCT TGTGAGGAAC CATCGCCAGG CGTCCTTGGA AATATCGCCA 
ATAACTTTCC TTACTGCTCA AACACTCTTG ATGGACCTTG GACAGTTTCT ACTGTTTTGT 
CATATCTCTT CCCACCAACA TGATGGCATG GAAGCTTATG TCAAAGTAGA CAGCTGCCCA 
GAGGAACCCC AACTACGAAT GAAAAATAAT GAAGAAGCGG AAGACTATGA TGATGATCTT 
ACTGATTCTG AAATGGATGT GGTCAGGTTT GATGATGACA ACTCTCCTTC CTTTATCCAA 
ATTCGCTCAG TTGCCAAGAA GCATCCTAAA ACTTGGGTAC ATTACATTGC TGCTGAAGAG 
GAGGACTGGG ACTATGCTCC CTTAGTCCTC GCCCCCGATG ACAGAAGTTA TAAAAGTCAA 
TATTTGAACA ATGGCCCTCA GCGGATTGGT AGGAAGTACA AAAAAGTCCG ATTTATGGCA 
TACACAGATG AAACCTTTAA GACTCGTGAA GCTATTCAGC ATGAATCAGG AATCTTGGGA 
CCTTTACTTT ATGGGGAAGT TGGAGACACA CTGTTGATTA TATTTAAGAA TCAAGCAAGC 
AGACCATATA ACATCTACCC TCACGGAATC ACTGATGTCC GTCCTTTGTA TTCAAGGAGA 
TTACCAAAAG GTGTAAAACA TTTGAAGGAT TTTCCAATTC TGCCAGGAGA AATATTCAAA 
TATAAATGGA CAGTGACTGT AGAAGATGGG CCAACTAAAT CAGATCCTCG GTGCCTGACC 
CGCTATTACT CTAGTTTCGT TAATATGGAG AGAGATCTAG CTTCAGGACT CATTGGCCCT 
CTCCTCATCT GCTACAAAGA ATCTGTAGAT CAAAGAGGAA ACCAGATAAT GTCAGACAAG 
AGGAATGTCA TCCTGTTTTC TGTATTTGAT GAGAACCGAA GCTGGTACCT CACAGAGAAT 
ATACAACGCT TTCTCCCCAA TCCAGCTGGA GTGCAGCTTG AGGATCCAGA GTTCCAAGCC 
TCCAACATCA TGCACAGCAT CAATGGCTAT GTTTTTGATA GTTTGCAGTT GTCAGTTTGT 
TTGCATGAGG TGGCATACTG GTACATTCTA AGCATTGGAG CACAGACTGA CTTCCTTTCT 
GTCTTCTTCT CTGGATATAC CTTCAAACAC AAAATGGTCT ATGAAGATAC ACTCACCCTA 
TTCCCATTCT CAGGAGAAAC TGTCTTCATG TCGATGGAAA ACCCAGGCCT ATGGATTCTG 
GGGTGCCACA ACTCAGACTT TCGGAACAGA GGCATGACCG CCTTACTGAA GGTTTCTAGT 
TGTGACAAGA ACACTGGTGA TTATTACGAG GACAGTTATG AAGATATTTC AGCATACTTG 
CTGAGTAAAA ACAATGCCAT TGAACCAAGA AGCTTCTCCC AGAATTCAAG ACACCCTAGC 
ACTAGGCAAA AGCAATTTAA TGCCACCACA ATTCCAGAAA ATGACATAGA GAAGACTGAC 
CCTTGGTTTG CACACAGAAC ACCTATGCCT AAAGTACAAA ATGTCTCCTC TAGTGATTTG 
TTGATGCTCT TGCGACAGAG TCCTACTCCA CATGGGCTAT CCTTATCTGA TCTCCAAGAA 
GCCAAATATG AGACTTTTTC TGATGATCCA TCACCTGGAG CAATAGACAG TAATAACAGC 
CTATCTGAAA TGACACACTT CAGGCCACAG TTCCATCACA GTGGGGACAT GGTATTTACC 
CCTGAGTCAG GCCTCCAATT GAGATTAAAT GAGAAACTGG GGACAACTGC AGCAACAGAG 
TTGAAGAAAC TTGATTTCAA AGTTTCTAGT ACATCAAATA ATCTGATTTC AACAATTCCA 
TCAGACAATT TGGCAGCAGG TACTGATAAT ACAAGTTCCT TAGGACCCCC AAGTATGCCA 
GTTCATTATG ATAGTCAATT AGATACCACT CTATTTGGCA AAAAGTCATC TCCCCTTACT 
GAGTCTGGTA GACCTCTGAG CTTGAGTGAA GAAAATAATG ATTCAAAGTT GTTAGAATCA 
GGTTTAATGA ATAGCCAAGA AAGTTCATGG GGAAAAAATG TATCGTCAAC AGAGAGTGGT 
AGGTTATTTA AAGGGAAAAG AGCTCATGGA CCTGCTTTGT TGACTAAAGA TAATGCCTTA 
TTCAAAGTTA GCATCTCTTT GTTAAAGACA AACAAAACTT CCAATAATTC AGCAACTAAT 
AGAAAGACTC ACATTGATGG CCCATCATTA TTAATTCAGA GTAGTCCATC AGTCTGGCAA 
AATATATTAG AAAGTGACAC TGAGTTTAAA AAAGTGACAC CTTTGATTCA TGACAGAATG 
CTTATGGACA AAAATGCTAC AGCTTTGAGG CTAAATCATA TGTCAAATAA AACTACTTCA 
TCAAAAAACA TGGAAATGGT CCGACAGAAA AAAGAGGGCC CCATTCCACC AGATGCAGAA 
AATCCAGATA TGTCATTCTT TAAGATGCTA TTCTTGCCAG AATCAGCAAG TTGGATACAA 
AGGACTCATG GAAAGAACTC TCTGAACTCT GGGCAAGGCC CCAGTCCAAA GCAATTAGTA 
TCCTTAGGAC CAGAAAAATC TGTGGAAGGT CAGAATTTCT TGTCTGAGAA AAACAAAGTG 
GTAGTAGGAA AGGGTGAATT TACAAAGGAC GTAGGACTCA AAGAGATGGT TTTTCCAAGC 
AGCAGAAACC TATTTCTTAC TAACTTGGAT AATTTACATG AAAATAATAC ACACAATCAA 
GAAAAAAAAA TTCAGGAAGA AATAGAAAGG AAGGAAACAT TAATCCAAGA GAATGTAGTT 
TTGCCTCAGA TACATACAGT GACTGGCACT AAGAATTTCA TGAAGAACCT TTTCTTACTG 
AGCACTAGGC AAAATGTAGA AGGTTCATAT GAGGGGGCAT ATGCTCCAGT ACTTCAAGAT 
TTTAGGTCAT TAAATGATTC AACAAATAGA ACAAAGAAAC ACACGGCTCA TTTCTCAAAA 
AAAGGGGAAG AAGAAAACTT GGCAGGCTTG GGAAATCAAA CCAAGCAAAT TGTAGAGAAA 
TATGCATGCA CCACAAGGAT ATCTCCTAAT ACAAGCCAGC AGAATTTTGT CACGCAACGT 
AGTAAGAGAG CTTTGAAACA ATTCAGACTC CCACTAGAAG AAACAGAACT TGAAAAAAGG 
ATAATTGTGG ATGACACCTC AACCCAGTGG TCCAAAAACA TGAAACATTT GACCCCGAGC 
ACCCTCACAC AGATAGACTA CAATGAGAAG GAGAAAGGGG CCATTACTCA GTCTCCCTTA 
TCAGATTGCC TTACGAGGAG TCATAGCATC CCTCAAGCAA ATAGATCTCC ATTACCCATT 
GCAGAGGTAT CATCATTTCC ATCTATTAGA CCTATATATC TGACCAGGGT CCTATTCCAA 
GACAACTCTT CTCATCTTCC AGCAGCATCT TATAGAAAGA AAGATTCTGG GGTCCAAGAA 
AGCAGTCATT TCTTACAAGG AGCCAAAAAA AATAACCTTT CTTTAGCCAT TCTAACCTTG 
GAGATGACTG GTGATCAAAG AGAGGTTGGC TCCCTGGGGA CAAGTGCCAC AAATTCAGTC 
ACTTACAAGA AAGTTGAGAA CACTGTTCTC CCGAAACCAG GCTTGCCCAA AACATCTGGC 
AAAGTTGAAT TGCTTCCAAA AGTTCACATT TATCAGAAGG ACCTATTCCC TACGGAAACT 
AGCAATGGGT CTCCTGGCCA TCTGGATCTC GTGGAAGGGA GCCTTCTTCA GGAAACAGAG 
GGAGCGATTA AGTGGAATGA AGCAAACAGA CCTGGAAAAA TTCCCTTTCT GAGAGTAGCA 
ACAGAAAGCT CTGCAAAGAC TCCCTCCAAG CTATTGGATC CTCTTGCTTG GGATAACCAC 
TATGGTACTC AGATACCAAA AGAAGAGTGG AAATCCCAAG AGAAGTCACC AGAAAAAACA 
GCTTTTAAGA AAAAGGATAC CATTTTGTCC CTGAACGCTT GTGAAAGCAA TCATGCAATA 
GCAGCAATAA ATGAGGGACA AAATAAGCCC GAAATAGAAG TCACCTGGGC AAAGCAAGGT 
AGGACTGAAA GGCTGTGCTC TCAAAACCCA CCAGGCTTGA AACGCCATCA ACGGGAAATA 
ACTCGTACTA CTCTTCAGTC AGATCAAGAG GAAATTGACT ATGATGATAC CATCTCAGTT 
GAAATGAAGA AGGAAGATTT TGACATTTAT GATGAGGATG AAAATCAGAG CCCCCGCAGC 
TTTCAAAAGA AAACACGACA CTATTTTATT GCTGCAGTGG AGAGGCTCTG GGATTATGGG 
ATGAGTAGCT CCCCACATGT TCTAAGAAAC AGGGCTCAGA GTGGCAGTGT CCCTCAGTTC 
AAGAAAGTTG TTTTCCAGGA ATTTACTGAT GGCTCCTTTA CTCAGCCCTT ATACCGTGGA 
GAACTAAATG AACATTTGGG ACTCCTGGGG CCATATATAA GAGCAGAAGT TGAAGATAAT 
ATCATGGTAA CTTTCAAAAA TCAGGCCTCT CGTCCCTATT CCTTCTATTC TAGCCTTATT 
TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGGA AAAACTTTGT CAAGCCTAAT 
GAAACCAAAA CTTACTTTTG GAAAGTGCAA CATCATATGG CGCCCACTAA AGATGAGTTT 
GACTGCAAAG CCTGGGCTTA TTTCTCTGAT GTTGACCTGG AAAAAGATGT GCACTCAGGC 
CTGATTGGAC CCCTTCTGGT CTGCCACACT AACACACTGA ACCCTGCTCA TGGGAGACAA 
GTGACAGTAC AGGAATTTGC TCTGTTTTTC ACCATCTTTG ATGAGACCAA AAGCTGGTAC 
TTCACTGAAA ACATGGAAAG AAACTGCAGG GCTCCCTGCA ATATCCAGAT GGAAGATCCC 
ACTTTTAAAG AGAATTATCG CTTCCATGCA ATCAATGGCT ACATAATGGA TACACTACCT 
GGCTTAGTAA TGGCTCAGGA TCAAAGGATT CGATGGTATC TGCTCAGCAT GGGCAGCAAT 
GAAAACATCC ATTCTATTCA TTTCAGTGGA CATGTGTTCA CTGTACGAAA AAAAGAGGAG 
TATAAAATGG CACTGTACAA TCTCTATCCA GGTGTTTTTG AGACAGTGGA AATGTTACCA 
TCCAAAGCTG GAATTTGGCG GGTGGAATGC CTTATTGGCG AGCATCTACA TGCTGGGATG 
AGCACACTTT TTCTGGTGTA CAGCAATAAG TGTCAGACTC CCCTGGGAAT GGCTTCTGGA 
CACATTAGAG ATTTTCAGAT TACAGCTTCA GGACAATATG GACAGTGGGC CCCAAAGCTG 
GCCAGACTTC ATTATTCCGG ATCAATCAAT GCCTGGAGCA CCAAGGAGCC CTTTTCTTGG 
ATCAAGGTGG ATCTGTTGGC ACCAATGATT ATTCACGGCA TCAAGACCCA GGGTGCCCGT 
CAGAAGTTCT CCAGCCTCTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGGAAG 
AAGTGGCAGA CTTATCGAGG AAATTCCACT GGAACCTTAA TGGTCTTCTT TGGCAATGTG 
GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCAA TTATTGCTCG ATACATCCGT 
TTGCACCCAA CTCATTATAG CATTCGCAGC ACTCTTCGCA TGGAGTTGAT GGGCTGTGAT 
TTAAATAGTT GCAGCATGCC ATTGGGAATG GAGAGTAAAG CAATATCAGA TGCACAGATT 
ACTGCTTCAT CCTACTTTAC CAATATGTTT GCCACCTGGT CTCCTTCAAA AGCTCGACTT 
CACCTCCAAG GGAGGAGTAA TGCCTGGAGA CCTCAGGTGA ATAATCCAAA AGAGTGGCTG 
CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAA CTACTCAGGG AGTAAAATCT 
CTGCTTACCA GCATGTATGT GAAGGAGTTC CTCATCTCCA GCAGTCAAGA TGGCCATCAC 
TGGACTCTCT TTTTTCAGAA TGGCAAAGTA AAGGTTTTTC AGGGAAATCA AGACTCCTTC 
ACACCTGTGG TGAACTCTCT AGACCCACCG TTACTGACTC GCTACCTTCG AATTCACCCC 
CAGAGTTGGG TGCACCAGAT TGCCCTGAGG ATGGAGGTTC TGGGCTGCGA GGCACAGGAC 
CTCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016800114.1
CDS	138..6866
Translation	MASHPVSLHAVGVSYWKASEGAEYDDQTSQREKEDDKVFPGGSHTYVWQVLKENGPMASDPLCLTYSYLSHVDLVKDLNSGLIGALLVCREGSLAKEKTQTLHKFILLFAVFDEGKSWHSETKNSLMQDRDAASARAWPKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTLLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKNNEEAEDYDDDLTDSEMDVVRFDDDNSPSFIQIRSVAKKHPKTWVHYIAAEEEDWDYAPLVLAPDDRSYKSQYLNNGPQRIGRKYKKVRFMAYTDETFKTREAIQHESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVRPLYSRRLPKGVKHLKDFPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFVNMERDLASGLIGPLLICYKESVDQRGNQIMSDKRNVILFSVFDENRSWYLTENIQRFLPNPAGVQLEDPEFQASNIMHSINGYVFDSLQLSVCLHEVAYWYILSIGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISAYLLSKNNAIEPRSFSQNSRHPSTRQKQFNATTIPENDIEKTDPWFAHRTPMPKVQNVSSSDLLMLLRQSPTPHGLSLSDLQEAKYETFSDDPSPGAIDSNNSLSEMTHFRPQFHHSGDMVFTPESGLQLRLNEKLGTTAATELKKLDFKVSSTSNNLISTIPSDNLAAGTDNTSSLGPPSMPVHYDSQLDTTLFGKKSSPLTESGRPLSLSEENNDSKLLESGLMNSQESSWGKNVSSTESGRLFKGKRAHGPALLTKDNALFKVSISLLKTNKTSNNSATNRKTHIDGPSLLIQSSPSVWQNILESDTEFKKVTPLIHDRMLMDKNATALRLNHMSNKTTSSKNMEMVRQKKEGPIPPDAENPDMSFFKMLFLPESASWIQRTHGKNSLNSGQGPSPKQLVSLGPEKSVEGQNFLSEKNKVVVGKGEFTKDVGLKEMVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIERKETLIQENVVLPQIHTVTGTKNFMKNLFLLSTRQNVEGSYEGAYAPVLQDFRSLNDSTNRTKKHTAHFSKKGEEENLAGLGNQTKQIVEKYACTTRISPNTSQQNFVTQRSKRALKQFRLPLEETELEKRIIVDDTSTQWSKNMKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSIPQANRSPLPIAEVSSFPSIRPIYLTRVLFQDNSSHLPAASYRKKDSGVQESSHFLQGAKKNNLSLAILTLEMTGDQREVGSLGTSATNSVTYKKVENTVLPKPGLPKTSGKVELLPKVHIYQKDLFPTETSNGSPGHLDLVEGSLLQETEGAIKWNEANRPGKIPFLRVATESSAKTPSKLLDPLAWDNHYGTQIPKEEWKSQEKSPEKTAFKKKDTILSLNACESNHAIAAINEGQNKPEIEVTWAKQGRTERLCSQNPPGLKRHQREITRTTLQSDQEEIDYDDTISVEMKKEDFDIYDEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSGSVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFDETKSWYFTENMERNCRAPCNIQMEDPTFKENYRFHAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMALYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGHIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTSMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY

Target ORF information:

RefSeq Version	XM_016944625.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes coagulation factor VIII (F8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016944625.1

ORF Insert Sequence:

ATGGCTTCCC ATCCTGTCAG TCTTCATGCT GTTGGTGTAT CCTACTGGAA AGCTTCTGAG 
GGAGCTGAAT ATGATGATCA GACCAGTCAA AGGGAGAAAG AAGATGATAA AGTCTTTCCT 
GGTGGAAGCC ATACATATGT CTGGCAGGTC CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC 
CCACTGTGCC TTACCTACTC ATATCTTTCT CATGTGGACC TGGTCAAAGA CTTGAATTCA 
GGCCTCATTG GAGCCCTACT AGTATGTAGA GAAGGGAGTC TGGCCAAGGA AAAGACACAG 
ACCTTGCACA AATTTATACT ACTTTTTGCT GTATTTGATG AAGGGAAAAG TTGGCACTCA 
GAAACAAAGA ACTCCTTGAT GCAGGATAGG GATGCTGCAT CTGCTCGGGC CTGGCCTAAA 
ATGCACACAG TCAATGGTTA TGTAAACAGG TCTCTGCCAG GTCTGATTGG ATGCCACAGG 
AAATCAGTCT ATTGGCATGT GATTGGAATG GGCACCACTC CTGAAGTGCA CTCAATATTC 
CTCGAAGGTC ACACATTTCT TGTGAGGAAC CATCGCCAGG CGTCCTTGGA AATATCGCCA 
ATAACTTTCC TTACTGCTCA AACACTCTTG ATGGACCTTG GACAGTTTCT ACTGTTTTGT 
CATATCTCTT CCCACCAACA TGATGGCATG GAAGCTTATG TCAAAGTAGA CAGCTGCCCA 
GAGGAACCCC AACTACGAAT GAAAAATAAT GAAGAAGCGG AAGACTATGA TGATGATCTT 
ACTGATTCTG AAATGGATGT GGTCAGGTTT GATGATGACA ACTCTCCTTC CTTTATCCAA 
ATTCGCTCAG TTGCCAAGAA GCATCCTAAA ACTTGGGTAC ATTACATTGC TGCTGAAGAG 
GAGGACTGGG ACTATGCTCC CTTAGTCCTC GCCCCCGATG ACAGAAGTTA TAAAAGTCAA 
TATTTGAACA ATGGCCCTCA GCGGATTGGT AGGAAGTACA AAAAAGTCCG ATTTATGGCA 
TACACAGATG AAACCTTTAA GACTCGTGAA GCTATTCAGC ATGAATCAGG AATCTTGGGA 
CCTTTACTTT ATGGGGAAGT TGGAGACACA CTGTTGATTA TATTTAAGAA TCAAGCAAGC 
AGACCATATA ACATCTACCC TCACGGAATC ACTGATGTCC GTCCTTTGTA TTCAAGGAGA 
TTACCAAAAG GTGTAAAACA TTTGAAGGAT TTTCCAATTC TGCCAGGAGA AATATTCAAA 
TATAAATGGA CAGTGACTGT AGAAGATGGG CCAACTAAAT CAGATCCTCG GTGCCTGACC 
CGCTATTACT CTAGTTTCGT TAATATGGAG AGAGATCTAG CTTCAGGACT CATTGGCCCT 
CTCCTCATCT GCTACAAAGA ATCTGTAGAT CAAAGAGGAA ACCAGATAAT GTCAGACAAG 
AGGAATGTCA TCCTGTTTTC TGTATTTGAT GAGAACCGAA GCTGGTACCT CACAGAGAAT 
ATACAACGCT TTCTCCCCAA TCCAGCTGGA GTGCAGCTTG AGGATCCAGA GTTCCAAGCC 
TCCAACATCA TGCACAGCAT CAATGGCTAT GTTTTTGATA GTTTGCAGTT GTCAGTTTGT 
TTGCATGAGG TGGCATACTG GTACATTCTA AGCATTGGAG CACAGACTGA CTTCCTTTCT 
GTCTTCTTCT CTGGATATAC CTTCAAACAC AAAATGGTCT ATGAAGATAC ACTCACCCTA 
TTCCCATTCT CAGGAGAAAC TGTCTTCATG TCGATGGAAA ACCCAGGCCT ATGGATTCTG 
GGGTGCCACA ACTCAGACTT TCGGAACAGA GGCATGACCG CCTTACTGAA GGTTTCTAGT 
TGTGACAAGA ACACTGGTGA TTATTACGAG GACAGTTATG AAGATATTTC AGCATACTTG 
CTGAGTAAAA ACAATGCCAT TGAACCAAGA AGCTTCTCCC AGAATTCAAG ACACCCTAGC 
ACTAGGCAAA AGCAATTTAA TGCCACCACA ATTCCAGAAA ATGACATAGA GAAGACTGAC 
CCTTGGTTTG CACACAGAAC ACCTATGCCT AAAGTACAAA ATGTCTCCTC TAGTGATTTG 
TTGATGCTCT TGCGACAGAG TCCTACTCCA CATGGGCTAT CCTTATCTGA TCTCCAAGAA 
GCCAAATATG AGACTTTTTC TGATGATCCA TCACCTGGAG CAATAGACAG TAATAACAGC 
CTATCTGAAA TGACACACTT CAGGCCACAG TTCCATCACA GTGGGGACAT GGTATTTACC 
CCTGAGTCAG GCCTCCAATT GAGATTAAAT GAGAAACTGG GGACAACTGC AGCAACAGAG 
TTGAAGAAAC TTGATTTCAA AGTTTCTAGT ACATCAAATA ATCTGATTTC AACAATTCCA 
TCAGACAATT TGGCAGCAGG TACTGATAAT ACAAGTTCCT TAGGACCCCC AAGTATGCCA 
GTTCATTATG ATAGTCAATT AGATACCACT CTATTTGGCA AAAAGTCATC TCCCCTTACT 
GAGTCTGGTA GACCTCTGAG CTTGAGTGAA GAAAATAATG ATTCAAAGTT GTTAGAATCA 
GGTTTAATGA ATAGCCAAGA AAGTTCATGG GGAAAAAATG TATCGTCAAC AGAGAGTGGT 
AGGTTATTTA AAGGGAAAAG AGCTCATGGA CCTGCTTTGT TGACTAAAGA TAATGCCTTA 
TTCAAAGTTA GCATCTCTTT GTTAAAGACA AACAAAACTT CCAATAATTC AGCAACTAAT 
AGAAAGACTC ACATTGATGG CCCATCATTA TTAATTCAGA GTAGTCCATC AGTCTGGCAA 
AATATATTAG AAAGTGACAC TGAGTTTAAA AAAGTGACAC CTTTGATTCA TGACAGAATG 
CTTATGGACA AAAATGCTAC AGCTTTGAGG CTAAATCATA TGTCAAATAA AACTACTTCA 
TCAAAAAACA TGGAAATGGT CCGACAGAAA AAAGAGGGCC CCATTCCACC AGATGCAGAA 
AATCCAGATA TGTCATTCTT TAAGATGCTA TTCTTGCCAG AATCAGCAAG TTGGATACAA 
AGGACTCATG GAAAGAACTC TCTGAACTCT GGGCAAGGCC CCAGTCCAAA GCAATTAGTA 
TCCTTAGGAC CAGAAAAATC TGTGGAAGGT CAGAATTTCT TGTCTGAGAA AAACAAAGTG 
GTAGTAGGAA AGGGTGAATT TACAAAGGAC GTAGGACTCA AAGAGATGGT TTTTCCAAGC 
AGCAGAAACC TATTTCTTAC TAACTTGGAT AATTTACATG AAAATAATAC ACACAATCAA 
GAAAAAAAAA TTCAGGAAGA AATAGAAAGG AAGGAAACAT TAATCCAAGA GAATGTAGTT 
TTGCCTCAGA TACATACAGT GACTGGCACT AAGAATTTCA TGAAGAACCT TTTCTTACTG 
AGCACTAGGC AAAATGTAGA AGGTTCATAT GAGGGGGCAT ATGCTCCAGT ACTTCAAGAT 
TTTAGGTCAT TAAATGATTC AACAAATAGA ACAAAGAAAC ACACGGCTCA TTTCTCAAAA 
AAAGGGGAAG AAGAAAACTT GGCAGGCTTG GGAAATCAAA CCAAGCAAAT TGTAGAGAAA 
TATGCATGCA CCACAAGGAT ATCTCCTAAT ACAAGCCAGC AGAATTTTGT CACGCAACGT 
AGTAAGAGAG CTTTGAAACA ATTCAGACTC CCACTAGAAG AAACAGAACT TGAAAAAAGG 
ATAATTGTGG ATGACACCTC AACCCAGTGG TCCAAAAACA TGAAACATTT GACCCCGAGC 
ACCCTCACAC AGATAGACTA CAATGAGAAG GAGAAAGGGG CCATTACTCA GTCTCCCTTA 
TCAGATTGCC TTACGAGGAG TCATAGCATC CCTCAAGCAA ATAGATCTCC ATTACCCATT 
GCAGAGGTAT CATCATTTCC ATCTATTAGA CCTATATATC TGACCAGGGT CCTATTCCAA 
GACAACTCTT CTCATCTTCC AGCAGCATCT TATAGAAAGA AAGATTCTGG GGTCCAAGAA 
AGCAGTCATT TCTTACAAGG AGCCAAAAAA AATAACCTTT CTTTAGCCAT TCTAACCTTG 
GAGATGACTG GTGATCAAAG AGAGGTTGGC TCCCTGGGGA CAAGTGCCAC AAATTCAGTC 
ACTTACAAGA AAGTTGAGAA CACTGTTCTC CCGAAACCAG GCTTGCCCAA AACATCTGGC 
AAAGTTGAAT TGCTTCCAAA AGTTCACATT TATCAGAAGG ACCTATTCCC TACGGAAACT 
AGCAATGGGT CTCCTGGCCA TCTGGATCTC GTGGAAGGGA GCCTTCTTCA GGAAACAGAG 
GGAGCGATTA AGTGGAATGA AGCAAACAGA CCTGGAAAAA TTCCCTTTCT GAGAGTAGCA 
ACAGAAAGCT CTGCAAAGAC TCCCTCCAAG CTATTGGATC CTCTTGCTTG GGATAACCAC 
TATGGTACTC AGATACCAAA AGAAGAGTGG AAATCCCAAG AGAAGTCACC AGAAAAAACA 
GCTTTTAAGA AAAAGGATAC CATTTTGTCC CTGAACGCTT GTGAAAGCAA TCATGCAATA 
GCAGCAATAA ATGAGGGACA AAATAAGCCC GAAATAGAAG TCACCTGGGC AAAGCAAGGT 
AGGACTGAAA GGCTGTGCTC TCAAAACCCA CCAGGCTTGA AACGCCATCA ACGGGAAATA 
ACTCGTACTA CTCTTCAGTC AGATCAAGAG GAAATTGACT ATGATGATAC CATCTCAGTT 
GAAATGAAGA AGGAAGATTT TGACATTTAT GATGAGGATG AAAATCAGAG CCCCCGCAGC 
TTTCAAAAGA AAACACGACA CTATTTTATT GCTGCAGTGG AGAGGCTCTG GGATTATGGG 
ATGAGTAGCT CCCCACATGT TCTAAGAAAC AGGGCTCAGA GTGGCAGTGT CCCTCAGTTC 
AAGAAAGTTG TTTTCCAGGA ATTTACTGAT GGCTCCTTTA CTCAGCCCTT ATACCGTGGA 
GAACTAAATG AACATTTGGG ACTCCTGGGG CCATATATAA GAGCAGAAGT TGAAGATAAT 
ATCATGGTAA CTTTCAAAAA TCAGGCCTCT CGTCCCTATT CCTTCTATTC TAGCCTTATT 
TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGGA AAAACTTTGT CAAGCCTAAT 
GAAACCAAAA CTTACTTTTG GAAAGTGCAA CATCATATGG CGCCCACTAA AGATGAGTTT 
GACTGCAAAG CCTGGGCTTA TTTCTCTGAT GTTGACCTGG AAAAAGATGT GCACTCAGGC 
CTGATTGGAC CCCTTCTGGT CTGCCACACT AACACACTGA ACCCTGCTCA TGGGAGACAA 
GTGACAGTAC AGGAATTTGC TCTGTTTTTC ACCATCTTTG ATGAGACCAA AAGCTGGTAC 
TTCACTGAAA ACATGGAAAG AAACTGCAGG GCTCCCTGCA ATATCCAGAT GGAAGATCCC 
ACTTTTAAAG AGAATTATCG CTTCCATGCA ATCAATGGCT ACATAATGGA TACACTACCT 
GGCTTAGTAA TGGCTCAGGA TCAAAGGATT CGATGGTATC TGCTCAGCAT GGGCAGCAAT 
GAAAACATCC ATTCTATTCA TTTCAGTGGA CATGTGTTCA CTGTACGAAA AAAAGAGGAG 
TATAAAATGG CACTGTACAA TCTCTATCCA GGTGTTTTTG AGACAGTGGA AATGTTACCA 
TCCAAAGCTG GAATTTGGCG GGTGGAATGC CTTATTGGCG AGCATCTACA TGCTGGGATG 
AGCACACTTT TTCTGGTGTA CAGCAATAAG TGTCAGACTC CCCTGGGAAT GGCTTCTGGA 
CACATTAGAG ATTTTCAGAT TACAGCTTCA GGACAATATG GACAGTGGGC CCCAAAGCTG 
GCCAGACTTC ATTATTCCGG ATCAATCAAT GCCTGGAGCA CCAAGGAGCC CTTTTCTTGG 
ATCAAGGTGG ATCTGTTGGC ACCAATGATT ATTCACGGCA TCAAGACCCA GGGTGCCCGT 
CAGAAGTTCT CCAGCCTCTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGGAAG 
AAGTGGCAGA CTTATCGAGG AAATTCCACT GGAACCTTAA TGGTCTTCTT TGGCAATGTG 
GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCAA TTATTGCTCG ATACATCCGT 
TTGCACCCAA CTCATTATAG CATTCGCAGC ACTCTTCGCA TGGAGTTGAT GGGCTGTGAT 
TTAAATAGTT GCAGCATGCC ATTGGGAATG GAGAGTAAAG CAATATCAGA TGCACAGATT 
ACTGCTTCAT CCTACTTTAC CAATATGTTT GCCACCTGGT CTCCTTCAAA AGCTCGACTT 
CACCTCCAAG GGAGGAGTAA TGCCTGGAGA CCTCAGGTGA ATAATCCAAA AGAGTGGCTG 
CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAA CTACTCAGGG AGTAAAATCT 
CTGCTTACCA GCATGTATGT GAAGGAGTTC CTCATCTCCA GCAGTCAAGA TGGCCATCAC 
TGGACTCTCT TTTTTCAGAA TGGCAAAGTA AAGGTTTTTC AGGGAAATCA AGACTCCTTC 
ACACCTGTGG TGAACTCTCT AGACCCACCG TTACTGACTC GCTACCTTCG AATTCACCCC 
CAGAGTTGGG TGCACCAGAT TGCCCTGAGG ATGGAGGTTC TGGGCTGCGA GGCACAGGAC 
CTCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_016944625.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016800114.1 Pan troglodytes coagulation factor VIII (F8), transcript variant X3, mRNA.

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CloneID	OPc91297
Clone ID Related Accession (Same CDS sequence)	XM_016944625.1 , XM_016944625.2
Accession Version	XM_016944625.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	coagulation factor VIII isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016944625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCC ATCCTGTCAG TCTTCATGCT GTTGGTGTAT CCTACTGGAA AGCTTCTGAG 
GGAGCTGAAT ATGATGATCA GACCAGTCAA AGGGAGAAAG AAGATGATAA AGTCTTTCCT 
GGTGGAAGCC ATACATATGT CTGGCAGGTC CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC 
CCACTGTGCC TTACCTACTC ATATCTTTCT CATGTGGACC TGGTCAAAGA CTTGAATTCA 
GGCCTCATTG GAGCCCTACT AGTATGTAGA GAAGGGAGTC TGGCCAAGGA AAAGACACAG 
ACCTTGCACA AATTTATACT ACTTTTTGCT GTATTTGATG AAGGGAAAAG TTGGCACTCA 
GAAACAAAGA ACTCCTTGAT GCAGGATAGG GATGCTGCAT CTGCTCGGGC CTGGCCTAAA 
ATGCACACAG TCAATGGTTA TGTAAACAGG TCTCTGCCAG GTCTGATTGG ATGCCACAGG 
AAATCAGTCT ATTGGCATGT GATTGGAATG GGCACCACTC CTGAAGTGCA CTCAATATTC 
CTCGAAGGTC ACACATTTCT TGTGAGGAAC CATCGCCAGG CGTCCTTGGA AATATCGCCA 
ATAACTTTCC TTACTGCTCA AACACTCTTG ATGGACCTTG GACAGTTTCT ACTGTTTTGT 
CATATCTCTT CCCACCAACA TGATGGCATG GAAGCTTATG TCAAAGTAGA CAGCTGCCCA 
GAGGAACCCC AACTACGAAT GAAAAATAAT GAAGAAGCGG AAGACTATGA TGATGATCTT 
ACTGATTCTG AAATGGATGT GGTCAGGTTT GATGATGACA ACTCTCCTTC CTTTATCCAA 
ATTCGCTCAG TTGCCAAGAA GCATCCTAAA ACTTGGGTAC ATTACATTGC TGCTGAAGAG 
GAGGACTGGG ACTATGCTCC CTTAGTCCTC GCCCCCGATG ACAGAAGTTA TAAAAGTCAA 
TATTTGAACA ATGGCCCTCA GCGGATTGGT AGGAAGTACA AAAAAGTCCG ATTTATGGCA 
TACACAGATG AAACCTTTAA GACTCGTGAA GCTATTCAGC ATGAATCAGG AATCTTGGGA 
CCTTTACTTT ATGGGGAAGT TGGAGACACA CTGTTGATTA TATTTAAGAA TCAAGCAAGC 
AGACCATATA ACATCTACCC TCACGGAATC ACTGATGTCC GTCCTTTGTA TTCAAGGAGA 
TTACCAAAAG GTGTAAAACA TTTGAAGGAT TTTCCAATTC TGCCAGGAGA AATATTCAAA 
TATAAATGGA CAGTGACTGT AGAAGATGGG CCAACTAAAT CAGATCCTCG GTGCCTGACC 
CGCTATTACT CTAGTTTCGT TAATATGGAG AGAGATCTAG CTTCAGGACT CATTGGCCCT 
CTCCTCATCT GCTACAAAGA ATCTGTAGAT CAAAGAGGAA ACCAGATAAT GTCAGACAAG 
AGGAATGTCA TCCTGTTTTC TGTATTTGAT GAGAACCGAA GCTGGTACCT CACAGAGAAT 
ATACAACGCT TTCTCCCCAA TCCAGCTGGA GTGCAGCTTG AGGATCCAGA GTTCCAAGCC 
TCCAACATCA TGCACAGCAT CAATGGCTAT GTTTTTGATA GTTTGCAGTT GTCAGTTTGT 
TTGCATGAGG TGGCATACTG GTACATTCTA AGCATTGGAG CACAGACTGA CTTCCTTTCT 
GTCTTCTTCT CTGGATATAC CTTCAAACAC AAAATGGTCT ATGAAGATAC ACTCACCCTA 
TTCCCATTCT CAGGAGAAAC TGTCTTCATG TCGATGGAAA ACCCAGGCCT ATGGATTCTG 
GGGTGCCACA ACTCAGACTT TCGGAACAGA GGCATGACCG CCTTACTGAA GGTTTCTAGT 
TGTGACAAGA ACACTGGTGA TTATTACGAG GACAGTTATG AAGATATTTC AGCATACTTG 
CTGAGTAAAA ACAATGCCAT TGAACCAAGA AGCTTCTCCC AGAATTCAAG ACACCCTAGC 
ACTAGGCAAA AGCAATTTAA TGCCACCACA ATTCCAGAAA ATGACATAGA GAAGACTGAC 
CCTTGGTTTG CACACAGAAC ACCTATGCCT AAAGTACAAA ATGTCTCCTC TAGTGATTTG 
TTGATGCTCT TGCGACAGAG TCCTACTCCA CATGGGCTAT CCTTATCTGA TCTCCAAGAA 
GCCAAATATG AGACTTTTTC TGATGATCCA TCACCTGGAG CAATAGACAG TAATAACAGC 
CTATCTGAAA TGACACACTT CAGGCCACAG TTCCATCACA GTGGGGACAT GGTATTTACC 
CCTGAGTCAG GCCTCCAATT GAGATTAAAT GAGAAACTGG GGACAACTGC AGCAACAGAG 
TTGAAGAAAC TTGATTTCAA AGTTTCTAGT ACATCAAATA ATCTGATTTC AACAATTCCA 
TCAGACAATT TGGCAGCAGG TACTGATAAT ACAAGTTCCT TAGGACCCCC AAGTATGCCA 
GTTCATTATG ATAGTCAATT AGATACCACT CTATTTGGCA AAAAGTCATC TCCCCTTACT 
GAGTCTGGTA GACCTCTGAG CTTGAGTGAA GAAAATAATG ATTCAAAGTT GTTAGAATCA 
GGTTTAATGA ATAGCCAAGA AAGTTCATGG GGAAAAAATG TATCGTCAAC AGAGAGTGGT 
AGGTTATTTA AAGGGAAAAG AGCTCATGGA CCTGCTTTGT TGACTAAAGA TAATGCCTTA 
TTCAAAGTTA GCATCTCTTT GTTAAAGACA AACAAAACTT CCAATAATTC AGCAACTAAT 
AGAAAGACTC ACATTGATGG CCCATCATTA TTAATTCAGA GTAGTCCATC AGTCTGGCAA 
AATATATTAG AAAGTGACAC TGAGTTTAAA AAAGTGACAC CTTTGATTCA TGACAGAATG 
CTTATGGACA AAAATGCTAC AGCTTTGAGG CTAAATCATA TGTCAAATAA AACTACTTCA 
TCAAAAAACA TGGAAATGGT CCGACAGAAA AAAGAGGGCC CCATTCCACC AGATGCAGAA 
AATCCAGATA TGTCATTCTT TAAGATGCTA TTCTTGCCAG AATCAGCAAG TTGGATACAA 
AGGACTCATG GAAAGAACTC TCTGAACTCT GGGCAAGGCC CCAGTCCAAA GCAATTAGTA 
TCCTTAGGAC CAGAAAAATC TGTGGAAGGT CAGAATTTCT TGTCTGAGAA AAACAAAGTG 
GTAGTAGGAA AGGGTGAATT TACAAAGGAC GTAGGACTCA AAGAGATGGT TTTTCCAAGC 
AGCAGAAACC TATTTCTTAC TAACTTGGAT AATTTACATG AAAATAATAC ACACAATCAA 
GAAAAAAAAA TTCAGGAAGA AATAGAAAGG AAGGAAACAT TAATCCAAGA GAATGTAGTT 
TTGCCTCAGA TACATACAGT GACTGGCACT AAGAATTTCA TGAAGAACCT TTTCTTACTG 
AGCACTAGGC AAAATGTAGA AGGTTCATAT GAGGGGGCAT ATGCTCCAGT ACTTCAAGAT 
TTTAGGTCAT TAAATGATTC AACAAATAGA ACAAAGAAAC ACACGGCTCA TTTCTCAAAA 
AAAGGGGAAG AAGAAAACTT GGCAGGCTTG GGAAATCAAA CCAAGCAAAT TGTAGAGAAA 
TATGCATGCA CCACAAGGAT ATCTCCTAAT ACAAGCCAGC AGAATTTTGT CACGCAACGT 
AGTAAGAGAG CTTTGAAACA ATTCAGACTC CCACTAGAAG AAACAGAACT TGAAAAAAGG 
ATAATTGTGG ATGACACCTC AACCCAGTGG TCCAAAAACA TGAAACATTT GACCCCGAGC 
ACCCTCACAC AGATAGACTA CAATGAGAAG GAGAAAGGGG CCATTACTCA GTCTCCCTTA 
TCAGATTGCC TTACGAGGAG TCATAGCATC CCTCAAGCAA ATAGATCTCC ATTACCCATT 
GCAGAGGTAT CATCATTTCC ATCTATTAGA CCTATATATC TGACCAGGGT CCTATTCCAA 
GACAACTCTT CTCATCTTCC AGCAGCATCT TATAGAAAGA AAGATTCTGG GGTCCAAGAA 
AGCAGTCATT TCTTACAAGG AGCCAAAAAA AATAACCTTT CTTTAGCCAT TCTAACCTTG 
GAGATGACTG GTGATCAAAG AGAGGTTGGC TCCCTGGGGA CAAGTGCCAC AAATTCAGTC 
ACTTACAAGA AAGTTGAGAA CACTGTTCTC CCGAAACCAG GCTTGCCCAA AACATCTGGC 
AAAGTTGAAT TGCTTCCAAA AGTTCACATT TATCAGAAGG ACCTATTCCC TACGGAAACT 
AGCAATGGGT CTCCTGGCCA TCTGGATCTC GTGGAAGGGA GCCTTCTTCA GGAAACAGAG 
GGAGCGATTA AGTGGAATGA AGCAAACAGA CCTGGAAAAA TTCCCTTTCT GAGAGTAGCA 
ACAGAAAGCT CTGCAAAGAC TCCCTCCAAG CTATTGGATC CTCTTGCTTG GGATAACCAC 
TATGGTACTC AGATACCAAA AGAAGAGTGG AAATCCCAAG AGAAGTCACC AGAAAAAACA 
GCTTTTAAGA AAAAGGATAC CATTTTGTCC CTGAACGCTT GTGAAAGCAA TCATGCAATA 
GCAGCAATAA ATGAGGGACA AAATAAGCCC GAAATAGAAG TCACCTGGGC AAAGCAAGGT 
AGGACTGAAA GGCTGTGCTC TCAAAACCCA CCAGGCTTGA AACGCCATCA ACGGGAAATA 
ACTCGTACTA CTCTTCAGTC AGATCAAGAG GAAATTGACT ATGATGATAC CATCTCAGTT 
GAAATGAAGA AGGAAGATTT TGACATTTAT GATGAGGATG AAAATCAGAG CCCCCGCAGC 
TTTCAAAAGA AAACACGACA CTATTTTATT GCTGCAGTGG AGAGGCTCTG GGATTATGGG 
ATGAGTAGCT CCCCACATGT TCTAAGAAAC AGGGCTCAGA GTGGCAGTGT CCCTCAGTTC 
AAGAAAGTTG TTTTCCAGGA ATTTACTGAT GGCTCCTTTA CTCAGCCCTT ATACCGTGGA 
GAACTAAATG AACATTTGGG ACTCCTGGGG CCATATATAA GAGCAGAAGT TGAAGATAAT 
ATCATGGTAA CTTTCAAAAA TCAGGCCTCT CGTCCCTATT CCTTCTATTC TAGCCTTATT 
TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGGA AAAACTTTGT CAAGCCTAAT 
GAAACCAAAA CTTACTTTTG GAAAGTGCAA CATCATATGG CGCCCACTAA AGATGAGTTT 
GACTGCAAAG CCTGGGCTTA TTTCTCTGAT GTTGACCTGG AAAAAGATGT GCACTCAGGC 
CTGATTGGAC CCCTTCTGGT CTGCCACACT AACACACTGA ACCCTGCTCA TGGGAGACAA 
GTGACAGTAC AGGAATTTGC TCTGTTTTTC ACCATCTTTG ATGAGACCAA AAGCTGGTAC 
TTCACTGAAA ACATGGAAAG AAACTGCAGG GCTCCCTGCA ATATCCAGAT GGAAGATCCC 
ACTTTTAAAG AGAATTATCG CTTCCATGCA ATCAATGGCT ACATAATGGA TACACTACCT 
GGCTTAGTAA TGGCTCAGGA TCAAAGGATT CGATGGTATC TGCTCAGCAT GGGCAGCAAT 
GAAAACATCC ATTCTATTCA TTTCAGTGGA CATGTGTTCA CTGTACGAAA AAAAGAGGAG 
TATAAAATGG CACTGTACAA TCTCTATCCA GGTGTTTTTG AGACAGTGGA AATGTTACCA 
TCCAAAGCTG GAATTTGGCG GGTGGAATGC CTTATTGGCG AGCATCTACA TGCTGGGATG 
AGCACACTTT TTCTGGTGTA CAGCAATAAG TGTCAGACTC CCCTGGGAAT GGCTTCTGGA 
CACATTAGAG ATTTTCAGAT TACAGCTTCA GGACAATATG GACAGTGGGC CCCAAAGCTG 
GCCAGACTTC ATTATTCCGG ATCAATCAAT GCCTGGAGCA CCAAGGAGCC CTTTTCTTGG 
ATCAAGGTGG ATCTGTTGGC ACCAATGATT ATTCACGGCA TCAAGACCCA GGGTGCCCGT 
CAGAAGTTCT CCAGCCTCTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGGAAG 
AAGTGGCAGA CTTATCGAGG AAATTCCACT GGAACCTTAA TGGTCTTCTT TGGCAATGTG 
GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCAA TTATTGCTCG ATACATCCGT 
TTGCACCCAA CTCATTATAG CATTCGCAGC ACTCTTCGCA TGGAGTTGAT GGGCTGTGAT 
TTAAATAGTT GCAGCATGCC ATTGGGAATG GAGAGTAAAG CAATATCAGA TGCACAGATT 
ACTGCTTCAT CCTACTTTAC CAATATGTTT GCCACCTGGT CTCCTTCAAA AGCTCGACTT 
CACCTCCAAG GGAGGAGTAA TGCCTGGAGA CCTCAGGTGA ATAATCCAAA AGAGTGGCTG 
CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAA CTACTCAGGG AGTAAAATCT 
CTGCTTACCA GCATGTATGT GAAGGAGTTC CTCATCTCCA GCAGTCAAGA TGGCCATCAC 
TGGACTCTCT TTTTTCAGAA TGGCAAAGTA AAGGTTTTTC AGGGAAATCA AGACTCCTTC 
ACACCTGTGG TGAACTCTCT AGACCCACCG TTACTGACTC GCTACCTTCG AATTCACCCC 
CAGAGTTGGG TGCACCAGAT TGCCCTGAGG ATGGAGGTTC TGGGCTGCGA GGCACAGGAC 
CTCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016800114.1
CDS	137..6865
Translation	MASHPVSLHAVGVSYWKASEGAEYDDQTSQREKEDDKVFPGGSHTYVWQVLKENGPMASDPLCLTYSYLSHVDLVKDLNSGLIGALLVCREGSLAKEKTQTLHKFILLFAVFDEGKSWHSETKNSLMQDRDAASARAWPKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTLLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKNNEEAEDYDDDLTDSEMDVVRFDDDNSPSFIQIRSVAKKHPKTWVHYIAAEEEDWDYAPLVLAPDDRSYKSQYLNNGPQRIGRKYKKVRFMAYTDETFKTREAIQHESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVRPLYSRRLPKGVKHLKDFPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFVNMERDLASGLIGPLLICYKESVDQRGNQIMSDKRNVILFSVFDENRSWYLTENIQRFLPNPAGVQLEDPEFQASNIMHSINGYVFDSLQLSVCLHEVAYWYILSIGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISAYLLSKNNAIEPRSFSQNSRHPSTRQKQFNATTIPENDIEKTDPWFAHRTPMPKVQNVSSSDLLMLLRQSPTPHGLSLSDLQEAKYETFSDDPSPGAIDSNNSLSEMTHFRPQFHHSGDMVFTPESGLQLRLNEKLGTTAATELKKLDFKVSSTSNNLISTIPSDNLAAGTDNTSSLGPPSMPVHYDSQLDTTLFGKKSSPLTESGRPLSLSEENNDSKLLESGLMNSQESSWGKNVSSTESGRLFKGKRAHGPALLTKDNALFKVSISLLKTNKTSNNSATNRKTHIDGPSLLIQSSPSVWQNILESDTEFKKVTPLIHDRMLMDKNATALRLNHMSNKTTSSKNMEMVRQKKEGPIPPDAENPDMSFFKMLFLPESASWIQRTHGKNSLNSGQGPSPKQLVSLGPEKSVEGQNFLSEKNKVVVGKGEFTKDVGLKEMVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIERKETLIQENVVLPQIHTVTGTKNFMKNLFLLSTRQNVEGSYEGAYAPVLQDFRSLNDSTNRTKKHTAHFSKKGEEENLAGLGNQTKQIVEKYACTTRISPNTSQQNFVTQRSKRALKQFRLPLEETELEKRIIVDDTSTQWSKNMKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSIPQANRSPLPIAEVSSFPSIRPIYLTRVLFQDNSSHLPAASYRKKDSGVQESSHFLQGAKKNNLSLAILTLEMTGDQREVGSLGTSATNSVTYKKVENTVLPKPGLPKTSGKVELLPKVHIYQKDLFPTETSNGSPGHLDLVEGSLLQETEGAIKWNEANRPGKIPFLRVATESSAKTPSKLLDPLAWDNHYGTQIPKEEWKSQEKSPEKTAFKKKDTILSLNACESNHAIAAINEGQNKPEIEVTWAKQGRTERLCSQNPPGLKRHQREITRTTLQSDQEEIDYDDTISVEMKKEDFDIYDEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSGSVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFDETKSWYFTENMERNCRAPCNIQMEDPTFKENYRFHAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMALYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGHIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTSMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY

Target ORF information:

RefSeq Version	XM_016944625.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes coagulation factor VIII (F8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016944625.2

ORF Insert Sequence:

ATGGCTTCCC ATCCTGTCAG TCTTCATGCT GTTGGTGTAT CCTACTGGAA AGCTTCTGAG 
GGAGCTGAAT ATGATGATCA GACCAGTCAA AGGGAGAAAG AAGATGATAA AGTCTTTCCT 
GGTGGAAGCC ATACATATGT CTGGCAGGTC CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC 
CCACTGTGCC TTACCTACTC ATATCTTTCT CATGTGGACC TGGTCAAAGA CTTGAATTCA 
GGCCTCATTG GAGCCCTACT AGTATGTAGA GAAGGGAGTC TGGCCAAGGA AAAGACACAG 
ACCTTGCACA AATTTATACT ACTTTTTGCT GTATTTGATG AAGGGAAAAG TTGGCACTCA 
GAAACAAAGA ACTCCTTGAT GCAGGATAGG GATGCTGCAT CTGCTCGGGC CTGGCCTAAA 
ATGCACACAG TCAATGGTTA TGTAAACAGG TCTCTGCCAG GTCTGATTGG ATGCCACAGG 
AAATCAGTCT ATTGGCATGT GATTGGAATG GGCACCACTC CTGAAGTGCA CTCAATATTC 
CTCGAAGGTC ACACATTTCT TGTGAGGAAC CATCGCCAGG CGTCCTTGGA AATATCGCCA 
ATAACTTTCC TTACTGCTCA AACACTCTTG ATGGACCTTG GACAGTTTCT ACTGTTTTGT 
CATATCTCTT CCCACCAACA TGATGGCATG GAAGCTTATG TCAAAGTAGA CAGCTGCCCA 
GAGGAACCCC AACTACGAAT GAAAAATAAT GAAGAAGCGG AAGACTATGA TGATGATCTT 
ACTGATTCTG AAATGGATGT GGTCAGGTTT GATGATGACA ACTCTCCTTC CTTTATCCAA 
ATTCGCTCAG TTGCCAAGAA GCATCCTAAA ACTTGGGTAC ATTACATTGC TGCTGAAGAG 
GAGGACTGGG ACTATGCTCC CTTAGTCCTC GCCCCCGATG ACAGAAGTTA TAAAAGTCAA 
TATTTGAACA ATGGCCCTCA GCGGATTGGT AGGAAGTACA AAAAAGTCCG ATTTATGGCA 
TACACAGATG AAACCTTTAA GACTCGTGAA GCTATTCAGC ATGAATCAGG AATCTTGGGA 
CCTTTACTTT ATGGGGAAGT TGGAGACACA CTGTTGATTA TATTTAAGAA TCAAGCAAGC 
AGACCATATA ACATCTACCC TCACGGAATC ACTGATGTCC GTCCTTTGTA TTCAAGGAGA 
TTACCAAAAG GTGTAAAACA TTTGAAGGAT TTTCCAATTC TGCCAGGAGA AATATTCAAA 
TATAAATGGA CAGTGACTGT AGAAGATGGG CCAACTAAAT CAGATCCTCG GTGCCTGACC 
CGCTATTACT CTAGTTTCGT TAATATGGAG AGAGATCTAG CTTCAGGACT CATTGGCCCT 
CTCCTCATCT GCTACAAAGA ATCTGTAGAT CAAAGAGGAA ACCAGATAAT GTCAGACAAG 
AGGAATGTCA TCCTGTTTTC TGTATTTGAT GAGAACCGAA GCTGGTACCT CACAGAGAAT 
ATACAACGCT TTCTCCCCAA TCCAGCTGGA GTGCAGCTTG AGGATCCAGA GTTCCAAGCC 
TCCAACATCA TGCACAGCAT CAATGGCTAT GTTTTTGATA GTTTGCAGTT GTCAGTTTGT 
TTGCATGAGG TGGCATACTG GTACATTCTA AGCATTGGAG CACAGACTGA CTTCCTTTCT 
GTCTTCTTCT CTGGATATAC CTTCAAACAC AAAATGGTCT ATGAAGATAC ACTCACCCTA 
TTCCCATTCT CAGGAGAAAC TGTCTTCATG TCGATGGAAA ACCCAGGCCT ATGGATTCTG 
GGGTGCCACA ACTCAGACTT TCGGAACAGA GGCATGACCG CCTTACTGAA GGTTTCTAGT 
TGTGACAAGA ACACTGGTGA TTATTACGAG GACAGTTATG AAGATATTTC AGCATACTTG 
CTGAGTAAAA ACAATGCCAT TGAACCAAGA AGCTTCTCCC AGAATTCAAG ACACCCTAGC 
ACTAGGCAAA AGCAATTTAA TGCCACCACA ATTCCAGAAA ATGACATAGA GAAGACTGAC 
CCTTGGTTTG CACACAGAAC ACCTATGCCT AAAGTACAAA ATGTCTCCTC TAGTGATTTG 
TTGATGCTCT TGCGACAGAG TCCTACTCCA CATGGGCTAT CCTTATCTGA TCTCCAAGAA 
GCCAAATATG AGACTTTTTC TGATGATCCA TCACCTGGAG CAATAGACAG TAATAACAGC 
CTATCTGAAA TGACACACTT CAGGCCACAG TTCCATCACA GTGGGGACAT GGTATTTACC 
CCTGAGTCAG GCCTCCAATT GAGATTAAAT GAGAAACTGG GGACAACTGC AGCAACAGAG 
TTGAAGAAAC TTGATTTCAA AGTTTCTAGT ACATCAAATA ATCTGATTTC AACAATTCCA 
TCAGACAATT TGGCAGCAGG TACTGATAAT ACAAGTTCCT TAGGACCCCC AAGTATGCCA 
GTTCATTATG ATAGTCAATT AGATACCACT CTATTTGGCA AAAAGTCATC TCCCCTTACT 
GAGTCTGGTA GACCTCTGAG CTTGAGTGAA GAAAATAATG ATTCAAAGTT GTTAGAATCA 
GGTTTAATGA ATAGCCAAGA AAGTTCATGG GGAAAAAATG TATCGTCAAC AGAGAGTGGT 
AGGTTATTTA AAGGGAAAAG AGCTCATGGA CCTGCTTTGT TGACTAAAGA TAATGCCTTA 
TTCAAAGTTA GCATCTCTTT GTTAAAGACA AACAAAACTT CCAATAATTC AGCAACTAAT 
AGAAAGACTC ACATTGATGG CCCATCATTA TTAATTCAGA GTAGTCCATC AGTCTGGCAA 
AATATATTAG AAAGTGACAC TGAGTTTAAA AAAGTGACAC CTTTGATTCA TGACAGAATG 
CTTATGGACA AAAATGCTAC AGCTTTGAGG CTAAATCATA TGTCAAATAA AACTACTTCA 
TCAAAAAACA TGGAAATGGT CCGACAGAAA AAAGAGGGCC CCATTCCACC AGATGCAGAA 
AATCCAGATA TGTCATTCTT TAAGATGCTA TTCTTGCCAG AATCAGCAAG TTGGATACAA 
AGGACTCATG GAAAGAACTC TCTGAACTCT GGGCAAGGCC CCAGTCCAAA GCAATTAGTA 
TCCTTAGGAC CAGAAAAATC TGTGGAAGGT CAGAATTTCT TGTCTGAGAA AAACAAAGTG 
GTAGTAGGAA AGGGTGAATT TACAAAGGAC GTAGGACTCA AAGAGATGGT TTTTCCAAGC 
AGCAGAAACC TATTTCTTAC TAACTTGGAT AATTTACATG AAAATAATAC ACACAATCAA 
GAAAAAAAAA TTCAGGAAGA AATAGAAAGG AAGGAAACAT TAATCCAAGA GAATGTAGTT 
TTGCCTCAGA TACATACAGT GACTGGCACT AAGAATTTCA TGAAGAACCT TTTCTTACTG 
AGCACTAGGC AAAATGTAGA AGGTTCATAT GAGGGGGCAT ATGCTCCAGT ACTTCAAGAT 
TTTAGGTCAT TAAATGATTC AACAAATAGA ACAAAGAAAC ACACGGCTCA TTTCTCAAAA 
AAAGGGGAAG AAGAAAACTT GGCAGGCTTG GGAAATCAAA CCAAGCAAAT TGTAGAGAAA 
TATGCATGCA CCACAAGGAT ATCTCCTAAT ACAAGCCAGC AGAATTTTGT CACGCAACGT 
AGTAAGAGAG CTTTGAAACA ATTCAGACTC CCACTAGAAG AAACAGAACT TGAAAAAAGG 
ATAATTGTGG ATGACACCTC AACCCAGTGG TCCAAAAACA TGAAACATTT GACCCCGAGC 
ACCCTCACAC AGATAGACTA CAATGAGAAG GAGAAAGGGG CCATTACTCA GTCTCCCTTA 
TCAGATTGCC TTACGAGGAG TCATAGCATC CCTCAAGCAA ATAGATCTCC ATTACCCATT 
GCAGAGGTAT CATCATTTCC ATCTATTAGA CCTATATATC TGACCAGGGT CCTATTCCAA 
GACAACTCTT CTCATCTTCC AGCAGCATCT TATAGAAAGA AAGATTCTGG GGTCCAAGAA 
AGCAGTCATT TCTTACAAGG AGCCAAAAAA AATAACCTTT CTTTAGCCAT TCTAACCTTG 
GAGATGACTG GTGATCAAAG AGAGGTTGGC TCCCTGGGGA CAAGTGCCAC AAATTCAGTC 
ACTTACAAGA AAGTTGAGAA CACTGTTCTC CCGAAACCAG GCTTGCCCAA AACATCTGGC 
AAAGTTGAAT TGCTTCCAAA AGTTCACATT TATCAGAAGG ACCTATTCCC TACGGAAACT 
AGCAATGGGT CTCCTGGCCA TCTGGATCTC GTGGAAGGGA GCCTTCTTCA GGAAACAGAG 
GGAGCGATTA AGTGGAATGA AGCAAACAGA CCTGGAAAAA TTCCCTTTCT GAGAGTAGCA 
ACAGAAAGCT CTGCAAAGAC TCCCTCCAAG CTATTGGATC CTCTTGCTTG GGATAACCAC 
TATGGTACTC AGATACCAAA AGAAGAGTGG AAATCCCAAG AGAAGTCACC AGAAAAAACA 
GCTTTTAAGA AAAAGGATAC CATTTTGTCC CTGAACGCTT GTGAAAGCAA TCATGCAATA 
GCAGCAATAA ATGAGGGACA AAATAAGCCC GAAATAGAAG TCACCTGGGC AAAGCAAGGT 
AGGACTGAAA GGCTGTGCTC TCAAAACCCA CCAGGCTTGA AACGCCATCA ACGGGAAATA 
ACTCGTACTA CTCTTCAGTC AGATCAAGAG GAAATTGACT ATGATGATAC CATCTCAGTT 
GAAATGAAGA AGGAAGATTT TGACATTTAT GATGAGGATG AAAATCAGAG CCCCCGCAGC 
TTTCAAAAGA AAACACGACA CTATTTTATT GCTGCAGTGG AGAGGCTCTG GGATTATGGG 
ATGAGTAGCT CCCCACATGT TCTAAGAAAC AGGGCTCAGA GTGGCAGTGT CCCTCAGTTC 
AAGAAAGTTG TTTTCCAGGA ATTTACTGAT GGCTCCTTTA CTCAGCCCTT ATACCGTGGA 
GAACTAAATG AACATTTGGG ACTCCTGGGG CCATATATAA GAGCAGAAGT TGAAGATAAT 
ATCATGGTAA CTTTCAAAAA TCAGGCCTCT CGTCCCTATT CCTTCTATTC TAGCCTTATT 
TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGGA AAAACTTTGT CAAGCCTAAT 
GAAACCAAAA CTTACTTTTG GAAAGTGCAA CATCATATGG CGCCCACTAA AGATGAGTTT 
GACTGCAAAG CCTGGGCTTA TTTCTCTGAT GTTGACCTGG AAAAAGATGT GCACTCAGGC 
CTGATTGGAC CCCTTCTGGT CTGCCACACT AACACACTGA ACCCTGCTCA TGGGAGACAA 
GTGACAGTAC AGGAATTTGC TCTGTTTTTC ACCATCTTTG ATGAGACCAA AAGCTGGTAC 
TTCACTGAAA ACATGGAAAG AAACTGCAGG GCTCCCTGCA ATATCCAGAT GGAAGATCCC 
ACTTTTAAAG AGAATTATCG CTTCCATGCA ATCAATGGCT ACATAATGGA TACACTACCT 
GGCTTAGTAA TGGCTCAGGA TCAAAGGATT CGATGGTATC TGCTCAGCAT GGGCAGCAAT 
GAAAACATCC ATTCTATTCA TTTCAGTGGA CATGTGTTCA CTGTACGAAA AAAAGAGGAG 
TATAAAATGG CACTGTACAA TCTCTATCCA GGTGTTTTTG AGACAGTGGA AATGTTACCA 
TCCAAAGCTG GAATTTGGCG GGTGGAATGC CTTATTGGCG AGCATCTACA TGCTGGGATG 
AGCACACTTT TTCTGGTGTA CAGCAATAAG TGTCAGACTC CCCTGGGAAT GGCTTCTGGA 
CACATTAGAG ATTTTCAGAT TACAGCTTCA GGACAATATG GACAGTGGGC CCCAAAGCTG 
GCCAGACTTC ATTATTCCGG ATCAATCAAT GCCTGGAGCA CCAAGGAGCC CTTTTCTTGG 
ATCAAGGTGG ATCTGTTGGC ACCAATGATT ATTCACGGCA TCAAGACCCA GGGTGCCCGT 
CAGAAGTTCT CCAGCCTCTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGGAAG 
AAGTGGCAGA CTTATCGAGG AAATTCCACT GGAACCTTAA TGGTCTTCTT TGGCAATGTG 
GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCAA TTATTGCTCG ATACATCCGT 
TTGCACCCAA CTCATTATAG CATTCGCAGC ACTCTTCGCA TGGAGTTGAT GGGCTGTGAT 
TTAAATAGTT GCAGCATGCC ATTGGGAATG GAGAGTAAAG CAATATCAGA TGCACAGATT 
ACTGCTTCAT CCTACTTTAC CAATATGTTT GCCACCTGGT CTCCTTCAAA AGCTCGACTT 
CACCTCCAAG GGAGGAGTAA TGCCTGGAGA CCTCAGGTGA ATAATCCAAA AGAGTGGCTG 
CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAA CTACTCAGGG AGTAAAATCT 
CTGCTTACCA GCATGTATGT GAAGGAGTTC CTCATCTCCA GCAGTCAAGA TGGCCATCAC 
TGGACTCTCT TTTTTCAGAA TGGCAAAGTA AAGGTTTTTC AGGGAAATCA AGACTCCTTC 
ACACCTGTGG TGAACTCTCT AGACCCACCG TTACTGACTC GCTACCTTCG AATTCACCCC 
CAGAGTTGGG TGCACCAGAT TGCCCTGAGG ATGGAGGTTC TGGGCTGCGA GGCACAGGAC 
CTCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_003317789.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_003317837.1 Pan troglodytes coagulation factor VIII (F8), transcript variant X1, mRNA.

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CloneID	OPc25432
Clone ID Related Accession (Same CDS sequence)	XM_003317789.3 , XM_003317789.4
Accession Version	XM_003317789.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	coagulation factor VIII isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_003317789.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAATAG AGCTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC 
GGTGAGCTGC CTGTGGACGC AAGATTTCCT CCTAGAGTGC CAAAATCTTT TCCATTCAAC 
ACCTCAGTCG TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC 
GCTAAGCCAA GGCCACCCTG GATGGGTCTG CTAGGTCCTA CCATCCAGGC TGAGGTTTAT 
GATACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT 
GGTGTATCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG 
GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG 
AAAGAGAATG GTCCAATGGC CTCTGACCCA CTGTGCCTTA CCTACTCATA TCTTTCTCAT 
GTGGACCTGG TCAAAGACTT GAATTCAGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA 
GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTATACTACT TTTTGCTGTA 
TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT 
GCTGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT 
CTGCCAGGTC TGATTGGATG CCACAGGAAA TCAGTCTATT GGCATGTGAT TGGAATGGGC 
ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT 
CGCCAGGCGT CCTTGGAAAT ATCGCCAATA ACTTTCCTTA CTGCTCAAAC ACTCTTGATG 
GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA 
GCTTATGTCA AAGTAGACAG CTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA 
GAAGCGGAAG ACTATGATGA TGATCTTACT GATTCTGAAA TGGATGTGGT CAGGTTTGAT 
GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT 
TGGGTACATT ACATTGCTGC TGAAGAGGAG GACTGGGACT ATGCTCCCTT AGTCCTCGCC 
CCCGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCCCTCAGCG GATTGGTAGG 
AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT 
ATTCAGCATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG 
TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT 
GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT 
CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA 
ACTAAATCAG ATCCTCGGTG CCTGACCCGC TATTACTCTA GTTTCGTTAA TATGGAGAGA 
GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA 
AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT ATTTGATGAG 
AACCGAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGCTGGAGTG 
CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT 
TTTGATAGTT TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC 
ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA 
ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC 
TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA 
GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG 
AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA 
TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA 
AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA 
TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA 
GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG 
CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA 
GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG 
GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA 
AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA 
AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC 
AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT 
CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT 
ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT 
AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG 
AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT 
CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT 
GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG 
AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC 
ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC 
ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT 
CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC 
AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA 
TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT 
GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT 
GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT 
ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT 
CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA 
CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC 
TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT 
CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT 
ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA 
ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC 
CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT 
CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG 
AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC 
ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT 
CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA 
GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC 
ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG 
GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA 
CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG 
GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003317837.1
CDS	398..7453
Translation	MQIELSTCFFLCLLRFCFSATRRYYLGAVELSWDYMQSDLGELPVDARFPPRVPKSFPFNTSVVYKKTVFVEFTDHLFNIAKPRPPWMGLLGPTIQAEVYDTVVITLKNMASHPVSLHAVGVSYWKASEGAEYDDQTSQREKEDDKVFPGGSHTYVWQVLKENGPMASDPLCLTYSYLSHVDLVKDLNSGLIGALLVCREGSLAKEKTQTLHKFILLFAVFDEGKSWHSETKNSLMQDRDAASARAWPKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTLLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKNNEEAEDYDDDLTDSEMDVVRFDDDNSPSFIQIRSVAKKHPKTWVHYIAAEEEDWDYAPLVLAPDDRSYKSQYLNNGPQRIGRKYKKVRFMAYTDETFKTREAIQHESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVRPLYSRRLPKGVKHLKDFPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFVNMERDLASGLIGPLLICYKESVDQRGNQIMSDKRNVILFSVFDENRSWYLTENIQRFLPNPAGVQLEDPEFQASNIMHSINGYVFDSLQLSVCLHEVAYWYILSIGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISAYLLSKNNAIEPRSFSQNSRHPSTRQKQFNATTIPENDIEKTDPWFAHRTPMPKVQNVSSSDLLMLLRQSPTPHGLSLSDLQEAKYETFSDDPSPGAIDSNNSLSEMTHFRPQFHHSGDMVFTPESGLQLRLNEKLGTTAATELKKLDFKVSSTSNNLISTIPSDNLAAGTDNTSSLGPPSMPVHYDSQLDTTLFGKKSSPLTESGRPLSLSEENNDSKLLESGLMNSQESSWGKNVSSTESGRLFKGKRAHGPALLTKDNALFKVSISLLKTNKTSNNSATNRKTHIDGPSLLIQSSPSVWQNILESDTEFKKVTPLIHDRMLMDKNATALRLNHMSNKTTSSKNMEMVRQKKEGPIPPDAENPDMSFFKMLFLPESASWIQRTHGKNSLNSGQGPSPKQLVSLGPEKSVEGQNFLSEKNKVVVGKGEFTKDVGLKEMVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIERKETLIQENVVLPQIHTVTGTKNFMKNLFLLSTRQNVEGSYEGAYAPVLQDFRSLNDSTNRTKKHTAHFSKKGEEENLAGLGNQTKQIVEKYACTTRISPNTSQQNFVTQRSKRALKQFRLPLEETELEKRIIVDDTSTQWSKNMKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSIPQANRSPLPIAEVSSFPSIRPIYLTRVLFQDNSSHLPAASYRKKDSGVQESSHFLQGAKKNNLSLAILTLEMTGDQREVGSLGTSATNSVTYKKVENTVLPKPGLPKTSGKVELLPKVHIYQKDLFPTETSNGSPGHLDLVEGSLLQETEGAIKWNEANRPGKIPFLRVATESSAKTPSKLLDPLAWDNHYGTQIPKEEWKSQEKSPEKTAFKKKDTILSLNACESNHAIAAINEGQNKPEIEVTWAKQGRTERLCSQNPPGLKRHQREITRTTLQSDQEEIDYDDTISVEMKKEDFDIYDEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSGSVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFDETKSWYFTENMERNCRAPCNIQMEDPTFKENYRFHAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMALYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGHIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTSMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY

Target ORF information:

RefSeq Version	XM_003317789.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes coagulation factor VIII (F8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003317789.3

ORF Insert Sequence:

ATGCAAATAG AGCTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC 
GGTGAGCTGC CTGTGGACGC AAGATTTCCT CCTAGAGTGC CAAAATCTTT TCCATTCAAC 
ACCTCAGTCG TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC 
GCTAAGCCAA GGCCACCCTG GATGGGTCTG CTAGGTCCTA CCATCCAGGC TGAGGTTTAT 
GATACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT 
GGTGTATCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG 
GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG 
AAAGAGAATG GTCCAATGGC CTCTGACCCA CTGTGCCTTA CCTACTCATA TCTTTCTCAT 
GTGGACCTGG TCAAAGACTT GAATTCAGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA 
GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTATACTACT TTTTGCTGTA 
TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT 
GCTGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT 
CTGCCAGGTC TGATTGGATG CCACAGGAAA TCAGTCTATT GGCATGTGAT TGGAATGGGC 
ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT 
CGCCAGGCGT CCTTGGAAAT ATCGCCAATA ACTTTCCTTA CTGCTCAAAC ACTCTTGATG 
GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA 
GCTTATGTCA AAGTAGACAG CTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA 
GAAGCGGAAG ACTATGATGA TGATCTTACT GATTCTGAAA TGGATGTGGT CAGGTTTGAT 
GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT 
TGGGTACATT ACATTGCTGC TGAAGAGGAG GACTGGGACT ATGCTCCCTT AGTCCTCGCC 
CCCGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCCCTCAGCG GATTGGTAGG 
AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT 
ATTCAGCATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG 
TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT 
GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT 
CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA 
ACTAAATCAG ATCCTCGGTG CCTGACCCGC TATTACTCTA GTTTCGTTAA TATGGAGAGA 
GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA 
AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT ATTTGATGAG 
AACCGAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGCTGGAGTG 
CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT 
TTTGATAGTT TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC 
ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA 
ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC 
TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA 
GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG 
AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA 
TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA 
AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA 
TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA 
GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG 
CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA 
GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG 
GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA 
AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA 
AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC 
AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT 
CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT 
ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT 
AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG 
AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT 
CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT 
GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG 
AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC 
ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC 
ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT 
CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC 
AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA 
TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT 
GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT 
GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT 
ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT 
CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA 
CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC 
TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT 
CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT 
ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA 
ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC 
CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT 
CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG 
AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC 
ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT 
CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA 
GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC 
ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG 
GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA 
CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG 
GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_003317789.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_003317837.1 Pan troglodytes coagulation factor VIII (F8), transcript variant X1, mRNA.

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CloneID	OPc25432
Clone ID Related Accession (Same CDS sequence)	XM_003317789.3 , XM_003317789.4
Accession Version	XM_003317789.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	coagulation factor VIII isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_003317789.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAATAG AGCTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC 
GGTGAGCTGC CTGTGGACGC AAGATTTCCT CCTAGAGTGC CAAAATCTTT TCCATTCAAC 
ACCTCAGTCG TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC 
GCTAAGCCAA GGCCACCCTG GATGGGTCTG CTAGGTCCTA CCATCCAGGC TGAGGTTTAT 
GATACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT 
GGTGTATCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG 
GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG 
AAAGAGAATG GTCCAATGGC CTCTGACCCA CTGTGCCTTA CCTACTCATA TCTTTCTCAT 
GTGGACCTGG TCAAAGACTT GAATTCAGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA 
GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTATACTACT TTTTGCTGTA 
TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT 
GCTGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT 
CTGCCAGGTC TGATTGGATG CCACAGGAAA TCAGTCTATT GGCATGTGAT TGGAATGGGC 
ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT 
CGCCAGGCGT CCTTGGAAAT ATCGCCAATA ACTTTCCTTA CTGCTCAAAC ACTCTTGATG 
GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA 
GCTTATGTCA AAGTAGACAG CTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA 
GAAGCGGAAG ACTATGATGA TGATCTTACT GATTCTGAAA TGGATGTGGT CAGGTTTGAT 
GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT 
TGGGTACATT ACATTGCTGC TGAAGAGGAG GACTGGGACT ATGCTCCCTT AGTCCTCGCC 
CCCGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCCCTCAGCG GATTGGTAGG 
AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT 
ATTCAGCATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG 
TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT 
GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT 
CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA 
ACTAAATCAG ATCCTCGGTG CCTGACCCGC TATTACTCTA GTTTCGTTAA TATGGAGAGA 
GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA 
AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT ATTTGATGAG 
AACCGAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGCTGGAGTG 
CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT 
TTTGATAGTT TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC 
ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA 
ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC 
TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA 
GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG 
AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA 
TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA 
AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA 
TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA 
GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG 
CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA 
GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG 
GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA 
AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA 
AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC 
AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT 
CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT 
ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT 
AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG 
AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT 
CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT 
GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG 
AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC 
ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC 
ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT 
CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC 
AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA 
TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT 
GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT 
GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT 
ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT 
CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA 
CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC 
TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT 
CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT 
ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA 
ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC 
CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT 
CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG 
AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC 
ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT 
CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA 
GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC 
ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG 
GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA 
CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG 
GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003317837.1
CDS	400..7455
Translation	MQIELSTCFFLCLLRFCFSATRRYYLGAVELSWDYMQSDLGELPVDARFPPRVPKSFPFNTSVVYKKTVFVEFTDHLFNIAKPRPPWMGLLGPTIQAEVYDTVVITLKNMASHPVSLHAVGVSYWKASEGAEYDDQTSQREKEDDKVFPGGSHTYVWQVLKENGPMASDPLCLTYSYLSHVDLVKDLNSGLIGALLVCREGSLAKEKTQTLHKFILLFAVFDEGKSWHSETKNSLMQDRDAASARAWPKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTLLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKNNEEAEDYDDDLTDSEMDVVRFDDDNSPSFIQIRSVAKKHPKTWVHYIAAEEEDWDYAPLVLAPDDRSYKSQYLNNGPQRIGRKYKKVRFMAYTDETFKTREAIQHESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVRPLYSRRLPKGVKHLKDFPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFVNMERDLASGLIGPLLICYKESVDQRGNQIMSDKRNVILFSVFDENRSWYLTENIQRFLPNPAGVQLEDPEFQASNIMHSINGYVFDSLQLSVCLHEVAYWYILSIGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISAYLLSKNNAIEPRSFSQNSRHPSTRQKQFNATTIPENDIEKTDPWFAHRTPMPKVQNVSSSDLLMLLRQSPTPHGLSLSDLQEAKYETFSDDPSPGAIDSNNSLSEMTHFRPQFHHSGDMVFTPESGLQLRLNEKLGTTAATELKKLDFKVSSTSNNLISTIPSDNLAAGTDNTSSLGPPSMPVHYDSQLDTTLFGKKSSPLTESGRPLSLSEENNDSKLLESGLMNSQESSWGKNVSSTESGRLFKGKRAHGPALLTKDNALFKVSISLLKTNKTSNNSATNRKTHIDGPSLLIQSSPSVWQNILESDTEFKKVTPLIHDRMLMDKNATALRLNHMSNKTTSSKNMEMVRQKKEGPIPPDAENPDMSFFKMLFLPESASWIQRTHGKNSLNSGQGPSPKQLVSLGPEKSVEGQNFLSEKNKVVVGKGEFTKDVGLKEMVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIERKETLIQENVVLPQIHTVTGTKNFMKNLFLLSTRQNVEGSYEGAYAPVLQDFRSLNDSTNRTKKHTAHFSKKGEEENLAGLGNQTKQIVEKYACTTRISPNTSQQNFVTQRSKRALKQFRLPLEETELEKRIIVDDTSTQWSKNMKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSIPQANRSPLPIAEVSSFPSIRPIYLTRVLFQDNSSHLPAASYRKKDSGVQESSHFLQGAKKNNLSLAILTLEMTGDQREVGSLGTSATNSVTYKKVENTVLPKPGLPKTSGKVELLPKVHIYQKDLFPTETSNGSPGHLDLVEGSLLQETEGAIKWNEANRPGKIPFLRVATESSAKTPSKLLDPLAWDNHYGTQIPKEEWKSQEKSPEKTAFKKKDTILSLNACESNHAIAAINEGQNKPEIEVTWAKQGRTERLCSQNPPGLKRHQREITRTTLQSDQEEIDYDDTISVEMKKEDFDIYDEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSGSVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFDETKSWYFTENMERNCRAPCNIQMEDPTFKENYRFHAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMALYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGHIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTSMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY

Target ORF information:

RefSeq Version	XM_003317789.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes coagulation factor VIII (F8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003317789.4

ORF Insert Sequence:

ATGCAAATAG AGCTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC 
GGTGAGCTGC CTGTGGACGC AAGATTTCCT CCTAGAGTGC CAAAATCTTT TCCATTCAAC 
ACCTCAGTCG TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC 
GCTAAGCCAA GGCCACCCTG GATGGGTCTG CTAGGTCCTA CCATCCAGGC TGAGGTTTAT 
GATACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT 
GGTGTATCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG 
GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG 
AAAGAGAATG GTCCAATGGC CTCTGACCCA CTGTGCCTTA CCTACTCATA TCTTTCTCAT 
GTGGACCTGG TCAAAGACTT GAATTCAGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA 
GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTATACTACT TTTTGCTGTA 
TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT 
GCTGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT 
CTGCCAGGTC TGATTGGATG CCACAGGAAA TCAGTCTATT GGCATGTGAT TGGAATGGGC 
ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT 
CGCCAGGCGT CCTTGGAAAT ATCGCCAATA ACTTTCCTTA CTGCTCAAAC ACTCTTGATG 
GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA 
GCTTATGTCA AAGTAGACAG CTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA 
GAAGCGGAAG ACTATGATGA TGATCTTACT GATTCTGAAA TGGATGTGGT CAGGTTTGAT 
GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT 
TGGGTACATT ACATTGCTGC TGAAGAGGAG GACTGGGACT ATGCTCCCTT AGTCCTCGCC 
CCCGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCCCTCAGCG GATTGGTAGG 
AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT 
ATTCAGCATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG 
TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT 
GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT 
CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA 
ACTAAATCAG ATCCTCGGTG CCTGACCCGC TATTACTCTA GTTTCGTTAA TATGGAGAGA 
GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA 
AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT ATTTGATGAG 
AACCGAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGCTGGAGTG 
CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT 
TTTGATAGTT TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC 
ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA 
ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC 
ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC 
AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC 
TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT 
CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA 
GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT 
GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA 
CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC 
CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG 
AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA 
TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA 
AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA 
TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA 
AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA 
AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT 
GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC 
AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA 
ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA 
GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA 
AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA 
GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC 
TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG 
CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG 
AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA 
GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT 
TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG 
GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG 
AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG 
GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA 
AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA 
AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA 
AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA 
CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC 
AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG 
AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT 
CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT 
ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT 
AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT 
AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC 
CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG 
AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT 
CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG 
GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT 
GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA 
TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA 
TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG 
AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA 
ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA 
GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA 
ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT 
GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT 
GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG 
GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC 
TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA 
TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT 
CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA 
CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT 
CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT 
GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC 
ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC 
ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT 
CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC 
AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA 
TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT 
GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT 
GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT 
ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT 
CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA 
CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC 
TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT 
CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT 
ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA 
ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC 
CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT 
CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG 
AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC 
ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT 
CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA 
GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC 
ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG 
GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA 
CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG 
GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_009439870.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009438145.1 Pan troglodytes coagulation factor VIII (F8), transcript variant X4, mRNA.

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CloneID	OPc46412
Clone ID Related Accession (Same CDS sequence)	XM_009439870.2
Accession Version	XM_009439870.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	coagulation factor VIII isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009439870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGATA GGGATGCTGC ATCTGCTCGG GCCTGGCCTA AAATGCACAC AGTCAATGGT 
TATGTAAACA GGTCTCTGCC AGGTCTGATT GGATGCCACA GGAAATCAGT CTATTGGCAT 
GTGATTGGAA TGGGCACCAC TCCTGAAGTG CACTCAATAT TCCTCGAAGG TCACACATTT 
CTTGTGAGGA ACCATCGCCA GGCGTCCTTG GAAATATCGC CAATAACTTT CCTTACTGCT 
CAAACACTCT TGATGGACCT TGGACAGTTT CTACTGTTTT GTCATATCTC TTCCCACCAA 
CATGATGGCA TGGAAGCTTA TGTCAAAGTA GACAGCTGCC CAGAGGAACC CCAACTACGA 
ATGAAAAATA ATGAAGAAGC GGAAGACTAT GATGATGATC TTACTGATTC TGAAATGGAT 
GTGGTCAGGT TTGATGATGA CAACTCTCCT TCCTTTATCC AAATTCGCTC AGTTGCCAAG 
AAGCATCCTA AAACTTGGGT ACATTACATT GCTGCTGAAG AGGAGGACTG GGACTATGCT 
CCCTTAGTCC TCGCCCCCGA TGACAGAAGT TATAAAAGTC AATATTTGAA CAATGGCCCT 
CAGCGGATTG GTAGGAAGTA CAAAAAAGTC CGATTTATGG CATACACAGA TGAAACCTTT 
AAGACTCGTG AAGCTATTCA GCATGAATCA GGAATCTTGG GACCTTTACT TTATGGGGAA 
GTTGGAGACA CACTGTTGAT TATATTTAAG AATCAAGCAA GCAGACCATA TAACATCTAC 
CCTCACGGAA TCACTGATGT CCGTCCTTTG TATTCAAGGA GATTACCAAA AGGTGTAAAA 
CATTTGAAGG ATTTTCCAAT TCTGCCAGGA GAAATATTCA AATATAAATG GACAGTGACT 
GTAGAAGATG GGCCAACTAA ATCAGATCCT CGGTGCCTGA CCCGCTATTA CTCTAGTTTC 
GTTAATATGG AGAGAGATCT AGCTTCAGGA CTCATTGGCC CTCTCCTCAT CTGCTACAAA 
GAATCTGTAG ATCAAAGAGG AAACCAGATA ATGTCAGACA AGAGGAATGT CATCCTGTTT 
TCTGTATTTG ATGAGAACCG AAGCTGGTAC CTCACAGAGA ATATACAACG CTTTCTCCCC 
AATCCAGCTG GAGTGCAGCT TGAGGATCCA GAGTTCCAAG CCTCCAACAT CATGCACAGC 
ATCAATGGCT ATGTTTTTGA TAGTTTGCAG TTGTCAGTTT GTTTGCATGA GGTGGCATAC 
TGGTACATTC TAAGCATTGG AGCACAGACT GACTTCCTTT CTGTCTTCTT CTCTGGATAT 
ACCTTCAAAC ACAAAATGGT CTATGAAGAT ACACTCACCC TATTCCCATT CTCAGGAGAA 
ACTGTCTTCA TGTCGATGGA AAACCCAGGC CTATGGATTC TGGGGTGCCA CAACTCAGAC 
TTTCGGAACA GAGGCATGAC CGCCTTACTG AAGGTTTCTA GTTGTGACAA GAACACTGGT 
GATTATTACG AGGACAGTTA TGAAGATATT TCAGCATACT TGCTGAGTAA AAACAATGCC 
ATTGAACCAA GAAGCTTCTC CCAGAATTCA AGACACCCTA GCACTAGGCA AAAGCAATTT 
AATGCCACCA CAATTCCAGA AAATGACATA GAGAAGACTG ACCCTTGGTT TGCACACAGA 
ACACCTATGC CTAAAGTACA AAATGTCTCC TCTAGTGATT TGTTGATGCT CTTGCGACAG 
AGTCCTACTC CACATGGGCT ATCCTTATCT GATCTCCAAG AAGCCAAATA TGAGACTTTT 
TCTGATGATC CATCACCTGG AGCAATAGAC AGTAATAACA GCCTATCTGA AATGACACAC 
TTCAGGCCAC AGTTCCATCA CAGTGGGGAC ATGGTATTTA CCCCTGAGTC AGGCCTCCAA 
TTGAGATTAA ATGAGAAACT GGGGACAACT GCAGCAACAG AGTTGAAGAA ACTTGATTTC 
AAAGTTTCTA GTACATCAAA TAATCTGATT TCAACAATTC CATCAGACAA TTTGGCAGCA 
GGTACTGATA ATACAAGTTC CTTAGGACCC CCAAGTATGC CAGTTCATTA TGATAGTCAA 
TTAGATACCA CTCTATTTGG CAAAAAGTCA TCTCCCCTTA CTGAGTCTGG TAGACCTCTG 
AGCTTGAGTG AAGAAAATAA TGATTCAAAG TTGTTAGAAT CAGGTTTAAT GAATAGCCAA 
GAAAGTTCAT GGGGAAAAAA TGTATCGTCA ACAGAGAGTG GTAGGTTATT TAAAGGGAAA 
AGAGCTCATG GACCTGCTTT GTTGACTAAA GATAATGCCT TATTCAAAGT TAGCATCTCT 
TTGTTAAAGA CAAACAAAAC TTCCAATAAT TCAGCAACTA ATAGAAAGAC TCACATTGAT 
GGCCCATCAT TATTAATTCA GAGTAGTCCA TCAGTCTGGC AAAATATATT AGAAAGTGAC 
ACTGAGTTTA AAAAAGTGAC ACCTTTGATT CATGACAGAA TGCTTATGGA CAAAAATGCT 
ACAGCTTTGA GGCTAAATCA TATGTCAAAT AAAACTACTT CATCAAAAAA CATGGAAATG 
GTCCGACAGA AAAAAGAGGG CCCCATTCCA CCAGATGCAG AAAATCCAGA TATGTCATTC 
TTTAAGATGC TATTCTTGCC AGAATCAGCA AGTTGGATAC AAAGGACTCA TGGAAAGAAC 
TCTCTGAACT CTGGGCAAGG CCCCAGTCCA AAGCAATTAG TATCCTTAGG ACCAGAAAAA 
TCTGTGGAAG GTCAGAATTT CTTGTCTGAG AAAAACAAAG TGGTAGTAGG AAAGGGTGAA 
TTTACAAAGG ACGTAGGACT CAAAGAGATG GTTTTTCCAA GCAGCAGAAA CCTATTTCTT 
ACTAACTTGG ATAATTTACA TGAAAATAAT ACACACAATC AAGAAAAAAA AATTCAGGAA 
GAAATAGAAA GGAAGGAAAC ATTAATCCAA GAGAATGTAG TTTTGCCTCA GATACATACA 
GTGACTGGCA CTAAGAATTT CATGAAGAAC CTTTTCTTAC TGAGCACTAG GCAAAATGTA 
GAAGGTTCAT ATGAGGGGGC ATATGCTCCA GTACTTCAAG ATTTTAGGTC ATTAAATGAT 
TCAACAAATA GAACAAAGAA ACACACGGCT CATTTCTCAA AAAAAGGGGA AGAAGAAAAC 
TTGGCAGGCT TGGGAAATCA AACCAAGCAA ATTGTAGAGA AATATGCATG CACCACAAGG 
ATATCTCCTA ATACAAGCCA GCAGAATTTT GTCACGCAAC GTAGTAAGAG AGCTTTGAAA 
CAATTCAGAC TCCCACTAGA AGAAACAGAA CTTGAAAAAA GGATAATTGT GGATGACACC 
TCAACCCAGT GGTCCAAAAA CATGAAACAT TTGACCCCGA GCACCCTCAC ACAGATAGAC 
TACAATGAGA AGGAGAAAGG GGCCATTACT CAGTCTCCCT TATCAGATTG CCTTACGAGG 
AGTCATAGCA TCCCTCAAGC AAATAGATCT CCATTACCCA TTGCAGAGGT ATCATCATTT 
CCATCTATTA GACCTATATA TCTGACCAGG GTCCTATTCC AAGACAACTC TTCTCATCTT 
CCAGCAGCAT CTTATAGAAA GAAAGATTCT GGGGTCCAAG AAAGCAGTCA TTTCTTACAA 
GGAGCCAAAA AAAATAACCT TTCTTTAGCC ATTCTAACCT TGGAGATGAC TGGTGATCAA 
AGAGAGGTTG GCTCCCTGGG GACAAGTGCC ACAAATTCAG TCACTTACAA GAAAGTTGAG 
AACACTGTTC TCCCGAAACC AGGCTTGCCC AAAACATCTG GCAAAGTTGA ATTGCTTCCA 
AAAGTTCACA TTTATCAGAA GGACCTATTC CCTACGGAAA CTAGCAATGG GTCTCCTGGC 
CATCTGGATC TCGTGGAAGG GAGCCTTCTT CAGGAAACAG AGGGAGCGAT TAAGTGGAAT 
GAAGCAAACA GACCTGGAAA AATTCCCTTT CTGAGAGTAG CAACAGAAAG CTCTGCAAAG 
ACTCCCTCCA AGCTATTGGA TCCTCTTGCT TGGGATAACC ACTATGGTAC TCAGATACCA 
AAAGAAGAGT GGAAATCCCA AGAGAAGTCA CCAGAAAAAA CAGCTTTTAA GAAAAAGGAT 
ACCATTTTGT CCCTGAACGC TTGTGAAAGC AATCATGCAA TAGCAGCAAT AAATGAGGGA 
CAAAATAAGC CCGAAATAGA AGTCACCTGG GCAAAGCAAG GTAGGACTGA AAGGCTGTGC 
TCTCAAAACC CACCAGGCTT GAAACGCCAT CAACGGGAAA TAACTCGTAC TACTCTTCAG 
TCAGATCAAG AGGAAATTGA CTATGATGAT ACCATCTCAG TTGAAATGAA GAAGGAAGAT 
TTTGACATTT ATGATGAGGA TGAAAATCAG AGCCCCCGCA GCTTTCAAAA GAAAACACGA 
CACTATTTTA TTGCTGCAGT GGAGAGGCTC TGGGATTATG GGATGAGTAG CTCCCCACAT 
GTTCTAAGAA ACAGGGCTCA GAGTGGCAGT GTCCCTCAGT TCAAGAAAGT TGTTTTCCAG 
GAATTTACTG ATGGCTCCTT TACTCAGCCC TTATACCGTG GAGAACTAAA TGAACATTTG 
GGACTCCTGG GGCCATATAT AAGAGCAGAA GTTGAAGATA ATATCATGGT AACTTTCAAA 
AATCAGGCCT CTCGTCCCTA TTCCTTCTAT TCTAGCCTTA TTTCTTATGA GGAAGATCAG 
AGGCAAGGAG CAGAACCTAG GAAAAACTTT GTCAAGCCTA ATGAAACCAA AACTTACTTT 
TGGAAAGTGC AACATCATAT GGCGCCCACT AAAGATGAGT TTGACTGCAA AGCCTGGGCT 
TATTTCTCTG ATGTTGACCT GGAAAAAGAT GTGCACTCAG GCCTGATTGG ACCCCTTCTG 
GTCTGCCACA CTAACACACT GAACCCTGCT CATGGGAGAC AAGTGACAGT ACAGGAATTT 
GCTCTGTTTT TCACCATCTT TGATGAGACC AAAAGCTGGT ACTTCACTGA AAACATGGAA 
AGAAACTGCA GGGCTCCCTG CAATATCCAG ATGGAAGATC CCACTTTTAA AGAGAATTAT 
CGCTTCCATG CAATCAATGG CTACATAATG GATACACTAC CTGGCTTAGT AATGGCTCAG 
GATCAAAGGA TTCGATGGTA TCTGCTCAGC ATGGGCAGCA ATGAAAACAT CCATTCTATT 
CATTTCAGTG GACATGTGTT CACTGTACGA AAAAAAGAGG AGTATAAAAT GGCACTGTAC 
AATCTCTATC CAGGTGTTTT TGAGACAGTG GAAATGTTAC CATCCAAAGC TGGAATTTGG 
CGGGTGGAAT GCCTTATTGG CGAGCATCTA CATGCTGGGA TGAGCACACT TTTTCTGGTG 
TACAGCAATA AGTGTCAGAC TCCCCTGGGA ATGGCTTCTG GACACATTAG AGATTTTCAG 
ATTACAGCTT CAGGACAATA TGGACAGTGG GCCCCAAAGC TGGCCAGACT TCATTATTCC 
GGATCAATCA ATGCCTGGAG CACCAAGGAG CCCTTTTCTT GGATCAAGGT GGATCTGTTG 
GCACCAATGA TTATTCACGG CATCAAGACC CAGGGTGCCC GTCAGAAGTT CTCCAGCCTC 
TACATCTCTC AGTTTATCAT CATGTATAGT CTTGATGGGA AGAAGTGGCA GACTTATCGA 
GGAAATTCCA CTGGAACCTT AATGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA 
CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT 
AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG 
CCATTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTT 
ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTCACCTCCA AGGGAGGAGT 
AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG 
ACAATGAAAG TCACAGGAAT AACTACTCAG GGAGTAAAAT CTCTGCTTAC CAGCATGTAT 
GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC ACTGGACTCT CTTTTTTCAG 
AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT 
CTAGACCCAC CGTTACTGAC TCGCTACCTT CGAATTCACC CCCAGAGTTG GGTGCACCAG 
ATTGCCCTGA GGATGGAGGT TCTGGGCTGC GAGGCACAGG ACCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009438145.1
CDS	206..6556
Translation	MQDRDAASARAWPKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTLLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKNNEEAEDYDDDLTDSEMDVVRFDDDNSPSFIQIRSVAKKHPKTWVHYIAAEEEDWDYAPLVLAPDDRSYKSQYLNNGPQRIGRKYKKVRFMAYTDETFKTREAIQHESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVRPLYSRRLPKGVKHLKDFPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFVNMERDLASGLIGPLLICYKESVDQRGNQIMSDKRNVILFSVFDENRSWYLTENIQRFLPNPAGVQLEDPEFQASNIMHSINGYVFDSLQLSVCLHEVAYWYILSIGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISAYLLSKNNAIEPRSFSQNSRHPSTRQKQFNATTIPENDIEKTDPWFAHRTPMPKVQNVSSSDLLMLLRQSPTPHGLSLSDLQEAKYETFSDDPSPGAIDSNNSLSEMTHFRPQFHHSGDMVFTPESGLQLRLNEKLGTTAATELKKLDFKVSSTSNNLISTIPSDNLAAGTDNTSSLGPPSMPVHYDSQLDTTLFGKKSSPLTESGRPLSLSEENNDSKLLESGLMNSQESSWGKNVSSTESGRLFKGKRAHGPALLTKDNALFKVSISLLKTNKTSNNSATNRKTHIDGPSLLIQSSPSVWQNILESDTEFKKVTPLIHDRMLMDKNATALRLNHMSNKTTSSKNMEMVRQKKEGPIPPDAENPDMSFFKMLFLPESASWIQRTHGKNSLNSGQGPSPKQLVSLGPEKSVEGQNFLSEKNKVVVGKGEFTKDVGLKEMVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIERKETLIQENVVLPQIHTVTGTKNFMKNLFLLSTRQNVEGSYEGAYAPVLQDFRSLNDSTNRTKKHTAHFSKKGEEENLAGLGNQTKQIVEKYACTTRISPNTSQQNFVTQRSKRALKQFRLPLEETELEKRIIVDDTSTQWSKNMKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSIPQANRSPLPIAEVSSFPSIRPIYLTRVLFQDNSSHLPAASYRKKDSGVQESSHFLQGAKKNNLSLAILTLEMTGDQREVGSLGTSATNSVTYKKVENTVLPKPGLPKTSGKVELLPKVHIYQKDLFPTETSNGSPGHLDLVEGSLLQETEGAIKWNEANRPGKIPFLRVATESSAKTPSKLLDPLAWDNHYGTQIPKEEWKSQEKSPEKTAFKKKDTILSLNACESNHAIAAINEGQNKPEIEVTWAKQGRTERLCSQNPPGLKRHQREITRTTLQSDQEEIDYDDTISVEMKKEDFDIYDEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSGSVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFDETKSWYFTENMERNCRAPCNIQMEDPTFKENYRFHAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMALYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASGHIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGARQKFSSLYISQFIIMYSLDGKKWQTYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTSMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY

Target ORF information:

RefSeq Version	XM_009439870.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes coagulation factor VIII (F8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009439870.2

ORF Insert Sequence:

ATGCAGGATA GGGATGCTGC ATCTGCTCGG GCCTGGCCTA AAATGCACAC AGTCAATGGT 
TATGTAAACA GGTCTCTGCC AGGTCTGATT GGATGCCACA GGAAATCAGT CTATTGGCAT 
GTGATTGGAA TGGGCACCAC TCCTGAAGTG CACTCAATAT TCCTCGAAGG TCACACATTT 
CTTGTGAGGA ACCATCGCCA GGCGTCCTTG GAAATATCGC CAATAACTTT CCTTACTGCT 
CAAACACTCT TGATGGACCT TGGACAGTTT CTACTGTTTT GTCATATCTC TTCCCACCAA 
CATGATGGCA TGGAAGCTTA TGTCAAAGTA GACAGCTGCC CAGAGGAACC CCAACTACGA 
ATGAAAAATA ATGAAGAAGC GGAAGACTAT GATGATGATC TTACTGATTC TGAAATGGAT 
GTGGTCAGGT TTGATGATGA CAACTCTCCT TCCTTTATCC AAATTCGCTC AGTTGCCAAG 
AAGCATCCTA AAACTTGGGT ACATTACATT GCTGCTGAAG AGGAGGACTG GGACTATGCT 
CCCTTAGTCC TCGCCCCCGA TGACAGAAGT TATAAAAGTC AATATTTGAA CAATGGCCCT 
CAGCGGATTG GTAGGAAGTA CAAAAAAGTC CGATTTATGG CATACACAGA TGAAACCTTT 
AAGACTCGTG AAGCTATTCA GCATGAATCA GGAATCTTGG GACCTTTACT TTATGGGGAA 
GTTGGAGACA CACTGTTGAT TATATTTAAG AATCAAGCAA GCAGACCATA TAACATCTAC 
CCTCACGGAA TCACTGATGT CCGTCCTTTG TATTCAAGGA GATTACCAAA AGGTGTAAAA 
CATTTGAAGG ATTTTCCAAT TCTGCCAGGA GAAATATTCA AATATAAATG GACAGTGACT 
GTAGAAGATG GGCCAACTAA ATCAGATCCT CGGTGCCTGA CCCGCTATTA CTCTAGTTTC 
GTTAATATGG AGAGAGATCT AGCTTCAGGA CTCATTGGCC CTCTCCTCAT CTGCTACAAA 
GAATCTGTAG ATCAAAGAGG AAACCAGATA ATGTCAGACA AGAGGAATGT CATCCTGTTT 
TCTGTATTTG ATGAGAACCG AAGCTGGTAC CTCACAGAGA ATATACAACG CTTTCTCCCC 
AATCCAGCTG GAGTGCAGCT TGAGGATCCA GAGTTCCAAG CCTCCAACAT CATGCACAGC 
ATCAATGGCT ATGTTTTTGA TAGTTTGCAG TTGTCAGTTT GTTTGCATGA GGTGGCATAC 
TGGTACATTC TAAGCATTGG AGCACAGACT GACTTCCTTT CTGTCTTCTT CTCTGGATAT 
ACCTTCAAAC ACAAAATGGT CTATGAAGAT ACACTCACCC TATTCCCATT CTCAGGAGAA 
ACTGTCTTCA TGTCGATGGA AAACCCAGGC CTATGGATTC TGGGGTGCCA CAACTCAGAC 
TTTCGGAACA GAGGCATGAC CGCCTTACTG AAGGTTTCTA GTTGTGACAA GAACACTGGT 
GATTATTACG AGGACAGTTA TGAAGATATT TCAGCATACT TGCTGAGTAA AAACAATGCC 
ATTGAACCAA GAAGCTTCTC CCAGAATTCA AGACACCCTA GCACTAGGCA AAAGCAATTT 
AATGCCACCA CAATTCCAGA AAATGACATA GAGAAGACTG ACCCTTGGTT TGCACACAGA 
ACACCTATGC CTAAAGTACA AAATGTCTCC TCTAGTGATT TGTTGATGCT CTTGCGACAG 
AGTCCTACTC CACATGGGCT ATCCTTATCT GATCTCCAAG AAGCCAAATA TGAGACTTTT 
TCTGATGATC CATCACCTGG AGCAATAGAC AGTAATAACA GCCTATCTGA AATGACACAC 
TTCAGGCCAC AGTTCCATCA CAGTGGGGAC ATGGTATTTA CCCCTGAGTC AGGCCTCCAA 
TTGAGATTAA ATGAGAAACT GGGGACAACT GCAGCAACAG AGTTGAAGAA ACTTGATTTC 
AAAGTTTCTA GTACATCAAA TAATCTGATT TCAACAATTC CATCAGACAA TTTGGCAGCA 
GGTACTGATA ATACAAGTTC CTTAGGACCC CCAAGTATGC CAGTTCATTA TGATAGTCAA 
TTAGATACCA CTCTATTTGG CAAAAAGTCA TCTCCCCTTA CTGAGTCTGG TAGACCTCTG 
AGCTTGAGTG AAGAAAATAA TGATTCAAAG TTGTTAGAAT CAGGTTTAAT GAATAGCCAA 
GAAAGTTCAT GGGGAAAAAA TGTATCGTCA ACAGAGAGTG GTAGGTTATT TAAAGGGAAA 
AGAGCTCATG GACCTGCTTT GTTGACTAAA GATAATGCCT TATTCAAAGT TAGCATCTCT 
TTGTTAAAGA CAAACAAAAC TTCCAATAAT TCAGCAACTA ATAGAAAGAC TCACATTGAT 
GGCCCATCAT TATTAATTCA GAGTAGTCCA TCAGTCTGGC AAAATATATT AGAAAGTGAC 
ACTGAGTTTA AAAAAGTGAC ACCTTTGATT CATGACAGAA TGCTTATGGA CAAAAATGCT 
ACAGCTTTGA GGCTAAATCA TATGTCAAAT AAAACTACTT CATCAAAAAA CATGGAAATG 
GTCCGACAGA AAAAAGAGGG CCCCATTCCA CCAGATGCAG AAAATCCAGA TATGTCATTC 
TTTAAGATGC TATTCTTGCC AGAATCAGCA AGTTGGATAC AAAGGACTCA TGGAAAGAAC 
TCTCTGAACT CTGGGCAAGG CCCCAGTCCA AAGCAATTAG TATCCTTAGG ACCAGAAAAA 
TCTGTGGAAG GTCAGAATTT CTTGTCTGAG AAAAACAAAG TGGTAGTAGG AAAGGGTGAA 
TTTACAAAGG ACGTAGGACT CAAAGAGATG GTTTTTCCAA GCAGCAGAAA CCTATTTCTT 
ACTAACTTGG ATAATTTACA TGAAAATAAT ACACACAATC AAGAAAAAAA AATTCAGGAA 
GAAATAGAAA GGAAGGAAAC ATTAATCCAA GAGAATGTAG TTTTGCCTCA GATACATACA 
GTGACTGGCA CTAAGAATTT CATGAAGAAC CTTTTCTTAC TGAGCACTAG GCAAAATGTA 
GAAGGTTCAT ATGAGGGGGC ATATGCTCCA GTACTTCAAG ATTTTAGGTC ATTAAATGAT 
TCAACAAATA GAACAAAGAA ACACACGGCT CATTTCTCAA AAAAAGGGGA AGAAGAAAAC 
TTGGCAGGCT TGGGAAATCA AACCAAGCAA ATTGTAGAGA AATATGCATG CACCACAAGG 
ATATCTCCTA ATACAAGCCA GCAGAATTTT GTCACGCAAC GTAGTAAGAG AGCTTTGAAA 
CAATTCAGAC TCCCACTAGA AGAAACAGAA CTTGAAAAAA GGATAATTGT GGATGACACC 
TCAACCCAGT GGTCCAAAAA CATGAAACAT TTGACCCCGA GCACCCTCAC ACAGATAGAC 
TACAATGAGA AGGAGAAAGG GGCCATTACT CAGTCTCCCT TATCAGATTG CCTTACGAGG 
AGTCATAGCA TCCCTCAAGC AAATAGATCT CCATTACCCA TTGCAGAGGT ATCATCATTT 
CCATCTATTA GACCTATATA TCTGACCAGG GTCCTATTCC AAGACAACTC TTCTCATCTT 
CCAGCAGCAT CTTATAGAAA GAAAGATTCT GGGGTCCAAG AAAGCAGTCA TTTCTTACAA 
GGAGCCAAAA AAAATAACCT TTCTTTAGCC ATTCTAACCT TGGAGATGAC TGGTGATCAA 
AGAGAGGTTG GCTCCCTGGG GACAAGTGCC ACAAATTCAG TCACTTACAA GAAAGTTGAG 
AACACTGTTC TCCCGAAACC AGGCTTGCCC AAAACATCTG GCAAAGTTGA ATTGCTTCCA 
AAAGTTCACA TTTATCAGAA GGACCTATTC CCTACGGAAA CTAGCAATGG GTCTCCTGGC 
CATCTGGATC TCGTGGAAGG GAGCCTTCTT CAGGAAACAG AGGGAGCGAT TAAGTGGAAT 
GAAGCAAACA GACCTGGAAA AATTCCCTTT CTGAGAGTAG CAACAGAAAG CTCTGCAAAG 
ACTCCCTCCA AGCTATTGGA TCCTCTTGCT TGGGATAACC ACTATGGTAC TCAGATACCA 
AAAGAAGAGT GGAAATCCCA AGAGAAGTCA CCAGAAAAAA CAGCTTTTAA GAAAAAGGAT 
ACCATTTTGT CCCTGAACGC TTGTGAAAGC AATCATGCAA TAGCAGCAAT AAATGAGGGA 
CAAAATAAGC CCGAAATAGA AGTCACCTGG GCAAAGCAAG GTAGGACTGA AAGGCTGTGC 
TCTCAAAACC CACCAGGCTT GAAACGCCAT CAACGGGAAA TAACTCGTAC TACTCTTCAG 
TCAGATCAAG AGGAAATTGA CTATGATGAT ACCATCTCAG TTGAAATGAA GAAGGAAGAT 
TTTGACATTT ATGATGAGGA TGAAAATCAG AGCCCCCGCA GCTTTCAAAA GAAAACACGA 
CACTATTTTA TTGCTGCAGT GGAGAGGCTC TGGGATTATG GGATGAGTAG CTCCCCACAT 
GTTCTAAGAA ACAGGGCTCA GAGTGGCAGT GTCCCTCAGT TCAAGAAAGT TGTTTTCCAG 
GAATTTACTG ATGGCTCCTT TACTCAGCCC TTATACCGTG GAGAACTAAA TGAACATTTG 
GGACTCCTGG GGCCATATAT AAGAGCAGAA GTTGAAGATA ATATCATGGT AACTTTCAAA 
AATCAGGCCT CTCGTCCCTA TTCCTTCTAT TCTAGCCTTA TTTCTTATGA GGAAGATCAG 
AGGCAAGGAG CAGAACCTAG GAAAAACTTT GTCAAGCCTA ATGAAACCAA AACTTACTTT 
TGGAAAGTGC AACATCATAT GGCGCCCACT AAAGATGAGT TTGACTGCAA AGCCTGGGCT 
TATTTCTCTG ATGTTGACCT GGAAAAAGAT GTGCACTCAG GCCTGATTGG ACCCCTTCTG 
GTCTGCCACA CTAACACACT GAACCCTGCT CATGGGAGAC AAGTGACAGT ACAGGAATTT 
GCTCTGTTTT TCACCATCTT TGATGAGACC AAAAGCTGGT ACTTCACTGA AAACATGGAA 
AGAAACTGCA GGGCTCCCTG CAATATCCAG ATGGAAGATC CCACTTTTAA AGAGAATTAT 
CGCTTCCATG CAATCAATGG CTACATAATG GATACACTAC CTGGCTTAGT AATGGCTCAG 
GATCAAAGGA TTCGATGGTA TCTGCTCAGC ATGGGCAGCA ATGAAAACAT CCATTCTATT 
CATTTCAGTG GACATGTGTT CACTGTACGA AAAAAAGAGG AGTATAAAAT GGCACTGTAC 
AATCTCTATC CAGGTGTTTT TGAGACAGTG GAAATGTTAC CATCCAAAGC TGGAATTTGG 
CGGGTGGAAT GCCTTATTGG CGAGCATCTA CATGCTGGGA TGAGCACACT TTTTCTGGTG 
TACAGCAATA AGTGTCAGAC TCCCCTGGGA ATGGCTTCTG GACACATTAG AGATTTTCAG 
ATTACAGCTT CAGGACAATA TGGACAGTGG GCCCCAAAGC TGGCCAGACT TCATTATTCC 
GGATCAATCA ATGCCTGGAG CACCAAGGAG CCCTTTTCTT GGATCAAGGT GGATCTGTTG 
GCACCAATGA TTATTCACGG CATCAAGACC CAGGGTGCCC GTCAGAAGTT CTCCAGCCTC 
TACATCTCTC AGTTTATCAT CATGTATAGT CTTGATGGGA AGAAGTGGCA GACTTATCGA 
GGAAATTCCA CTGGAACCTT AATGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA 
CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT 
AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG 
CCATTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTT 
ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTCACCTCCA AGGGAGGAGT 
AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG 
ACAATGAAAG TCACAGGAAT AACTACTCAG GGAGTAAAAT CTCTGCTTAC CAGCATGTAT 
GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC ACTGGACTCT CTTTTTTCAG 
AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT 
CTAGACCCAC CGTTACTGAC TCGCTACCTT CGAATTCACC CCCAGAGTTG GGTGCACCAG 
ATTGCCCTGA GGATGGAGGT TCTGGGCTGC GAGGCACAGG ACCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_003317788.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_003317836.2 Pan troglodytes coagulation factor VIII (F8), transcript variant X7, mRNA.

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CloneID	OPc91300
Clone ID Related Accession (Same CDS sequence)	XM_003317788.3
Accession Version	XM_003317788.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	coagulation factor VIII isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_003317788.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGCCG CCCCAAGCAC TTCCCGACGC GCCGCCGCAG CTGGCGGGCG GGCCGGGGCG 
GGGCGACGTG CCCTGCGTCC CCCTCGGCGG GCTGCCGCCG TGCCCGCGCC GGCTCCCCAG 
CCCGAGCCTG CCCCTTGCCC TGGTGATATG CAAAGAGCGG GATCGGAGGC GGGGCCTGGC 
CGGGCTGTGA GCGGCGTATG CAAATGGAGG GTCTCGGGGA TGCGGAGCCA AGACCCTGGG 
AAGGTCTTCT TTGGCAATGT GGATTCATCT GGGATAAAAC ACAATATTTT TAACCCTCCA 
ATTATTGCTC GATACATCCG TTTGCACCCA ACTCATTATA GCATTCGCAG CACTCTTCGC 
ATGGAGTTGA TGGGCTGTGA TTTAAATAGT TGCAGCATGC CATTGGGAAT GGAGAGTAAA 
GCAATATCAG ATGCACAGAT TACTGCTTCA TCCTACTTTA CCAATATGTT TGCCACCTGG 
TCTCCTTCAA AAGCTCGACT TCACCTCCAA GGGAGGAGTA ATGCCTGGAG ACCTCAGGTG 
AATAATCCAA AAGAGTGGCT GCAAGTGGAC TTCCAGAAGA CAATGAAAGT CACAGGAATA 
ACTACTCAGG GAGTAAAATC TCTGCTTACC AGCATGTATG TGAAGGAGTT CCTCATCTCC 
AGCAGTCAAG ATGGCCATCA CTGGACTCTC TTTTTTCAGA ATGGCAAAGT AAAGGTTTTT 
CAGGGAAATC AAGACTCCTT CACACCTGTG GTGAACTCTC TAGACCCACC GTTACTGACT 
CGCTACCTTC GAATTCACCC CCAGAGTTGG GTGCACCAGA TTGCCCTGAG GATGGAGGTT 
CTGGGCTGCG AGGCACAGGA CCTCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003317836.2
CDS	24..893
Translation	MLAAPSTSRRAAAAGGRAGAGRRALRPPRRAAAVPAPAPQPEPAPCPGDMQRAGSEAGPGRAVSGVCKWRVSGMRSQDPGKVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGITTQGVKSLLTSMYVKEFLISSSQDGHHWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY

Target ORF information:

RefSeq Version	XM_003317788.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes coagulation factor VIII (F8), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003317788.3

ORF Insert Sequence:

ATGCTCGCCG CCCCAAGCAC TTCCCGACGC GCCGCCGCAG CTGGCGGGCG GGCCGGGGCG 
GGGCGACGTG CCCTGCGTCC CCCTCGGCGG GCTGCCGCCG TGCCCGCGCC GGCTCCCCAG 
CCCGAGCCTG CCCCTTGCCC TGGTGATATG CAAAGAGCGG GATCGGAGGC GGGGCCTGGC 
CGGGCTGTGA GCGGCGTATG CAAATGGAGG GTCTCGGGGA TGCGGAGCCA AGACCCTGGG 
AAGGTCTTCT TTGGCAATGT GGATTCATCT GGGATAAAAC ACAATATTTT TAACCCTCCA 
ATTATTGCTC GATACATCCG TTTGCACCCA ACTCATTATA GCATTCGCAG CACTCTTCGC 
ATGGAGTTGA TGGGCTGTGA TTTAAATAGT TGCAGCATGC CATTGGGAAT GGAGAGTAAA 
GCAATATCAG ATGCACAGAT TACTGCTTCA TCCTACTTTA CCAATATGTT TGCCACCTGG 
TCTCCTTCAA AAGCTCGACT TCACCTCCAA GGGAGGAGTA ATGCCTGGAG ACCTCAGGTG 
AATAATCCAA AAGAGTGGCT GCAAGTGGAC TTCCAGAAGA CAATGAAAGT CACAGGAATA 
ACTACTCAGG GAGTAAAATC TCTGCTTACC AGCATGTATG TGAAGGAGTT CCTCATCTCC 
AGCAGTCAAG ATGGCCATCA CTGGACTCTC TTTTTTCAGA ATGGCAAAGT AAAGGTTTTT 
CAGGGAAATC AAGACTCCTT CACACCTGTG GTGAACTCTC TAGACCCACC GTTACTGACT 
CGCTACCTTC GAATTCACCC CCAGAGTTGG GTGCACCAGA TTGCCCTGAG GATGGAGGTT 
CTGGGCTGCG AGGCACAGGA CCTCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	F8
Entrez Gene ID	473838
Full Name	coagulation factor VIII
General protein information	Preferred Names coagulation factor VIII Names coagulation factor VIII coagulation factor VIII, procoagulant component
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

Homo sapiens (human)	F8	NP_000123.1
Canis lupus familiaris (dog)	F8	NP_001003212.1
Pan troglodytes (chimpanzee)	F8	XP_003317837.1
	LOC100424151	XP_002806506.1
Rattus norvegicus (Norway rat)	F8	NP_899160.1
Mus musculus (house mouse)	F8	NP_032003.2
Bos taurus (cattle)	F8	NP_001138980.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

F8 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

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