F8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	F8
Entrez Gene ID	302470
Full Name	coagulation factor VIII
General protein information	Preferred Names coagulation factor VIII Names coagulation factor VIII coagulation factor VIII, procoagulant component
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18p13
Summary	human homolog is a cofactor for factor IXa; involved in blood coagulation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_183331.1	NP_899160.1	coagulation factor VIII precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	F8	NP_000123.1
Canis lupus familiaris (dog)	F8	NP_001003212.1
Pan troglodytes (chimpanzee)	F8	XP_003317837.1
	LOC100424151	XP_002806506.1
Rattus norvegicus (Norway rat)	F8	NP_899160.1
Mus musculus (house mouse)	F8	NP_032003.2
Bos taurus (cattle)	F8	NP_001138980.1

Gene Ontology
Bibliography

Related articles in PubMed

Altered collagen turnover in factor VIII-deficient rats with hemophilic arthropathy identifies potential novel serological biomarkers in hemophilia.
Manon-Jensen T, Karsdal MA, Nielsen LN, Kjelgaard-Hansen M, Vandahl B, Olsen EH, Enoksson M, Roepstorff K
Journal of thrombosis and haemostasis : JTH14(12)2419-2429(2016 Dec)

How it all starts: Initiation of the clotting cascade.
Smith SA, Travers RJ, Morrissey JH
Critical reviews in biochemistry and molecular biology50(4)326-36(2015)

A novel F8 -/- rat as a translational model of human hemophilia A.
Nielsen LN, Wiinberg B, H?ger M, Holmberg HL, Hansen JJ, Roepstorff K, Tranholm M
Journal of thrombosis and haemostasis : JTH12(8)1274-82(2014 Aug)

WAG-F8(m1Ycb) rats harboring a factor VIII gene mutation provide a new animal model for hemophilia A.
Booth CJ, Brooks MB, Rockwell S, Murphy JW, Rinder HM, Zelterman D, Paidas MJ, Compton SR, Marks PW
Journal of thrombosis and haemostasis : JTH8(11)2472-7(2010 Nov)

Detection of 95 novel mutations in coagulation factor VIII gene F8 responsible for hemophilia A: results from a single institution.
Guillet B, Lambert T, d'Oiron R, Proulle V, Plantier JL, Rafowicz A, Peynet J, Costa JM, Bendelac L, Laurian Y, Lavergne JM
Human mutation27(7)676-85(2006 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

analysis of a F8(-/-) rat model of hemophilic arthropathy reveals that collagen turnover is altered
Title: Altered collagen turnover in factor VIII-deficient rats with hemophilic arthropathy identifies potential novel serological biomarkers in hemophilia.

analysis of animal models for hemophilia A and the advantage of rats over mice in the factor VIII gene mutation model
Title: WAG-F8(m1Ycb) rats harboring a factor VIII gene mutation provide a new animal model for hemophilia A.

Sequence of the rat factor VIII cDNA
Title: Sequence of the rat factor VIII cDNA.

The following F8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04679	NM_183331.1 Latest version!	Rattus norvegicus coagulation factor VIII (F8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F8 ( NM_183331.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_899160.1 Rattus norvegicus coagulation factor VIII (F8), mRNA.

Price & Availability↑

CloneID	ORa04679
Clone ID Related Accession (Same CDS sequence)	NM_183331.1
Accession Version	NM_183331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	coagulation factor VIII precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY362193.1. On Dec 17, 2003 this sequence version replaced XM_217580.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY362193.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCGCGCGG CGCGCGGCCT GTGCTTCTTC CTCAGCCTCT GCGCGCTCTG CTCCTGCGCC 
ACCCGGAAGT ACTACCTGGG CGCCGTGGAG TTGCCATGGG ACTATGTGAG CGGTGGTGCT 
TCCGGTGCCC AGCGCAGCGA CTCCAGGTTC CCTCCCGGGA CACCAGCGCC GTCTGCGCAT 
GTTGTGCACA CGAAGACGGT GTTTGTGGAG TACATGGACC GGTTTTTCCA CACGGCAAAG 
CCCAGGCCAC TGTGGATGGG TCTCCTAGGC CCCACCATTT GGACCGAGGT TCACGACACT 
GTGGTCATCA CACTCAAAAA CATGGCTTCT CATCCCGTCA GTCTCCACGC TGTCGGTATG 
TCCTTCTGGA AGGCGTCCGA GGGCGCGGCA TACGACGACC ACAGCAGCCC GGCCGAGAAG 
GACGACGACA AAGTCCTCCC CGGCGAGAGC CACACTTACG CGTGGCAGGT CCTGGGGGAG 
AGCGGCCCAA TGGCTTCCGA CCCCCCGTGC CTCACCTATG CCTACCTGTC TCATGTGGAC 
CTCGTGAGGG ACCTGAACTC GGGCCTCGTC GGGGCCCTGC TCGTGTGCAA AGAAGGCCGT 
CTGTCCGCAG AGAGAACACG GATGCCACCC CACTTCGTGC TGCTTTTTGC TGTCTTCGAT 
GAAGGGCGGA GCTGGCACGC AGCAACACGT GACCCCGCGT CCACCGAGGC CCAGCCCACG 
GTGCACACTG TGAATGGCTA CACGAACCGG ACACTGCCGG GTCTGACCGG ATGCCGCGGA 
ACATCCGTGT ACTGGCACGT GATGGCGGTG GGCACCACGC CCGACATACA CTCCATCCTC 
CTCGAAGGCC ACACATTCTC TGTGAGGAGC CACCGTCAGG CTTCCCTGGA GATCTCACCT 
ATGACTCTGC TCACGGCTCA GACGTGGCTG ATGGACCTGG GGCGGTTCCT GCTCTTCTGT 
CACATCTCCT CCCACAGACA CGGTGGCATG GAAGCCTACG TCCAGGTGGA CAACTGCCCC 
GAGGCGCCCC AGGGGCAGGG GAAAAGGGAC GAGGACACAG AGGACTATGA TGATCTGGAC 
TCGGAAATGG ACGTCTTCAC CTGGGACCCC GATGCCGCCC CCTTCGTCCA GGTCCGCTCA 
GCAGCCAAGA GACACCCCAA GACCTGGGTG CACTACATCG CAGCAGAGGA GGGGGACTGG 
GACTACGCGC CTGCGGCCGC CACCCTAGAA GACGGGAGCC TGCGGAACCG GTACCTGGGG 
CGCGGCCCTG GCCGCATCGG CAGCAAATAT AAGAAAGTCA GGTTCGTGGC CTACACGGAC 
GGCACCTTTC ACACGCGTGA GGCCACCCCG CGTGAAGCTG GGCTCCTAGG GCCACTGCTC 
TACGGGGAGG TCGGAGACTC ACTGTTGATT GTGTTCAAGA ACCGAGCGAG CCGGGCATAC 
AACATCCACC CTCATGGGAT CCGTGACGTT GGCGCTGTGC ATGCAGGGAG GCTGCCACGA 
GGGGTGAAGC ACGTGAAGGA TTTGCCGATC CGCCCAGGAG AGACGTTCAA GTACAGGTGG 
ACGCTGACCG CAGAGGATGG GCCTGCAAGG TCGGATGCGC GGTGTGTGAC GCGCTACTAT 
GCGAGCGCTG TGGATCCCGA GAGAGACCTG GCTTCCGGGC TGATTGGCCC CCTCCTCATC 
TGCTGTAAAG AATCTGTAGA TCAAAGGGGG AACCAGATGA TGTCAGACGA GAGGAACGTC 
ATCCTGTTCT CCGTGTTTGA TGAGAACCGG AGCTGGTACA TCTCGGAGAA CATGCGACGC 
TTCCTCCCCG ACGAGGCACA TGTGCAGCTG CAGGACCCCG AGTTCGAGGC CTCCAACCTC 
ATGCACAGCA TTAATGGCTA CGTCTTCGAT GGCCTGGAGC TGCGCGTTTG CCTGCACGAG 
GTGGCTGCCT GGCACGTGCT CAGCGTCGGC GCGCAGACTG ACTTCCTGTC TGTCTTCTTC 
TCTGGACACA CGTTCAGACA CAGGGCGGTG TACGCAGACA CACTCACGGT GTTCCCGCAC 
TCGGGGGTCA CCGTCTTCAT GTCCATGGAT AACCCAGGTG TGTGGGTGCT GGGGTGCCAC 
AACCCGGACT TCCGGGAGAG CGGGATGACC GCACTGCTGA AGGTTTCCAG TTGTGACGAG 
GGCGTGAGTG ATTACTACGG AGAGACCTAT GAGGGCGTTC TGGCCCCGTT GGCCAATGAC 
AACGCCGTGG ACCCCAGGAG CTTCTCCCAG AATTCAAATC ATCTGCACAG CAGGAAAAAG 
AAAAGCAACA CGCCGATGCG CTTCCCACTG AGCCATCCTA CCCAGCATGG CTCCTTTTGG 
TCGGACAGCC AGGGAGATGT CCATGACGCT GTTCATAGAG CCCATTCACC CGCTGCAGCA 
CACAGCAATG AAGGCCCAGC CGAAGCAGCC CAGCTCGAGC CCAAACCCCA TCCCGGTGAG 
AAAATGGCAC TTCCTCCTTC TTGGTTAAGA GCAAAGAGAA GTCTGGTGAC AACCATGGAA 
GCCAAGCGGA AGAAACTGGA TCTCCAGGTC CAGGTTTCTG GTTTGCCCGA CGACAGAACA 
ACCGCAGTGG TTGCGCCGGA CGATCCGATG GCGGCTTGTA AGAAGGCGGG TTCTTCAGGG 
TTTCCAGATG AATCGAGTCC CGCTGCATTG GGTAAGAAAA TGTATCCCCG TATCAGGCCC 
CACGGACCTT TAAGCATCAT TGAAGGAAAC AGGGATTCCA ACTCGTCGGA CTCGACTTTA 
ATGTATAGGC TGGGAAGTCC ACCAGGAGAC GCCACATCAT GGACGGAGAA CCGCAGAGGA 
CTTGGCGAGA AGAGGTCTCA CAGGGTCGCT TTCCTGGCCA GAGGTAACAC TTTACTCAGC 
GACGTTGATG AAAAATCGCA TGCCCCCGGC CCAACATCAG TTGGGAACAG CACAGCAGCC 
GTGCAGGACA CCATATTAGA GATCTGCAGT GAGATTCTCG AGGTCACACC TCCGATTCAT 
GACAGGATAC TTTCAGACAA AAGTGCTACA TATTTGAGAC CGCACCGTAC GCCCGACAGA 
ATCACCTCGA CGGAAAGGAA AGACATACGT CACGAAAAAG ATGGAGATCT TGTTCCGCAG 
GATGCAGATG ACACAAGCGC ACCATTCTCG GAGGCGCCTT TCCTGTCAGA ATCCACAGAT 
TGGTTAAAAG AGGCCAATGG GGATAACTCC GCAAAACCTG AGCAAGAACC CAGTCCAGAG 
CAATTAGTGT ATTTAATGTA TATAAAGTGT ATGGAAAATC AGAGTTTCTC GTCAGAGAAG 
AATAAAATGA TAGCAGGGCA AGGTGGATTT ACCAAGAACA CAGGACTTGA AGACACGGTT 
TTTCCCAGAA AGACGAGTGT GTTTCTCACC ACTGTGGCTA AGCGGCAAGA AAGTGGGAGG 
CACAACCAAG AAAACATTCC TCAGGCAGTG ACAGAGAAGG AAGCGCCAAT CGAAGAGAAG 
GGAGCTCTGC CCCAGGTGCA CATAGCGACT GGCTCTAAGA ACTTCCCGAG AGACATGTTT 
GTACTCGGTA CTGGGCAAAA TATAAATCTC CACGAAGAGA CATATGTACC AGTTCACAAA 
AACGTCCCAT GGACAAGAAA CCCAACAGAC ACAAGACAGA TTCCCATGGT TCATTTCTTT 
AAGAAAAGGA AGGAGGAGGA AACCAATTCA GGAGGCTTGG TAAATAAAAC GAGAGAAACG 
GTGAGAAACT ACCCAAGCCA GAAGAATAGC GTTGCTCGAC GTAGGGAACA GGCTTCGGGA 
CGAATCAAAG CATCCGCTCG TTGGCTTCCA GACGTAAACC GTTCAATCCA GAGTCTCCTT 
AAACAGATTG ACCACCGCAA GGAAAGGAAA AAGTTCATTA TAGAATCTCG CTCCGCAGAT 
TCTTCTGGGA CTAAAAGTCC CACTCAGACC AATCATTCTC CCTCACACGT TGTGAAAATG 
TCAGCATTCC CACCGAGAGA CATCCGAAGG ATCGAGTCGC GAGACAGTTC TCAGGTCTGG 
GTGTCGTCCT ATGCTTACGA CTTTGAGACA GGAAGTTCTA GAATTCGAGA GAGCAGTCCT 
TTCCTGAATG AAACTGAAAC GGATAACCCT TCTCTCGCCG TGCCACCGCG GGAGAGGTTC 
GTGCGTCGAG GAAGGTTCGC CTCCCCAGAA AAAGTGAACA CACACTCAGC GCCGTGTGAC 
AAACCCGAAA ATGTTGTCTC CTCGGAACCA GTGTCGCCTG GCGAAGCTGT CACAGTCGCA 
CTCCCTCCTC ACGTCTCCAC TCAGGAGGAG GAACCTTTGC CTGCAGGAAG TAGCTGTGAG 
CGTCGCGGAC ATGTGGATCT CTTTCTCGAG ATCTCTCTCC AGAGAACACG GGGGCCCGTT 
GAACGGAGTA GAGGAAGGAG GTCTGGGGGA GACACGGAAG GTCACACAGA GAACCCCGGA 
AAGACTCCCT CCCCACTGCC ACAGATGCCG AAAGATCAGC GTGGGTCTGA AGCGGGGTAC 
CCGAAAATCT GCCGCTGGAG AGACGGCACT GTTTTGCCCC CGAGGCCTCA CAACCTTTCC 
CTAGGAGCAA AGAAAAAACC AAATCTGCCC CGAAGAGAAG CCACTCGGGT GGAGCAAGGC 
GAGGCTCCGA GCCTGGTCTC TCCAGAGCCC CTGGTGCTGA GACGACGTCC AAGGGAAGCT 
AGCACCCTTC TGCCAGGAGG AGAGATGCGA GAGGACGACG GTGATGGTGT CACGGCGGAG 
TACGACGACG ACGATGACGT CATGGCAGAG TATGACGACG ATGCTGTCAC GGTGGACACG 
CCCGAGGATT TCGACATCTA TGGCGAGGAG GCAGGGCAGG GACCCCGCGG CTTTCAGCAG 
AAAACGCGGC ACTATTTCAT CGCGGCCGTG GAGGAGCTTT GGGACTACGG GGTGCGTGTG 
TCGCCTGGAG CTCTGGGAGA CAGGGCGTGG AGTGGGGACG CAGCTCGGTT CCGGAAAGTG 
GTTTTCCGTG AGTTCACGGA CGGGTCGTTC ACGCAGCGTG TGCACCGTGG GGAGCTGGAC 
GCACACCTGG GGCTATTGGG GCCATACATA CGGGCGGAAG TGGAGGACAA CATCGTGGTG 
ACCTTCCGGA ACCAGGCCTC CCGCCCCTAC TCCTTCTACT CCAGCCTCGT GTCCTACCCG 
GAAGCCGACG GTGGAGCTGC ACCAAGGAGC AACTTTGTGC GGCCCAACGA GACCAAAACT 
TACTTCTGGA GAGTCCGGCC CCACATGGCG CCCACGGACG GCGAGTTTGA CTGCAAGGCC 
TGGGCCTATT TCTCCGACGT GGACCTCGAG CGAGACCTGC ACTCGGGGCT GATTGGGCCA 
CTGCTCGTGT GCCACGCCTC CACCCTGCAC CCCTCGCACG CGCGGCCGCT CGCAGTGCAG 
GAGTTCGCGC TGCTCTTCGC GGTGTTCGAT GAGACCAAGA GCTGGTACTG CGCTGAGAAC 
CTGGACCGGA AGTGCAGGCC GCCCCGTGGC ACCCCCGCGG GAGACCCCGC CTGGAGAGAA 
GAGTACCGCT TCCATGCCGT GAATGGTTAC GTCGCAGATG CTCTGCTTGG CCTGGTGATG 
GCGGAAGGTC GGAGGACGAG GTGGCATCTG CTCAGCATGG GCGACCCCGG GCACGCACAG 
TCCGTGCATT TCAGCGCGCA CAGCGTCACC GTGCGGGACG GGGGCGAGCA CAGGACGGCG 
GTGTGCAACC TGTACCCAGG CGTGTTCACA ACCGTGGAGA TGTTGCCGTC CAGAGCGGGG 
ATCTGGCGGG TGGAGAGCCT GGTGGGCGAG CACCTACGGG CTGGGATGAG CGCCCTCTTC 
CTGGTGTACA GCACGCGCTG CCAGGTCCCG CTCGGCATGG CTTCCGGTTA CATCCGGGAC 
TCGCAGATCA CGGCTTCCGG CTACCACGGG CTGTGGACCC CGCAGCTCGC GAGGCTTCAC 
AACGCGGGAT CCGTGAATGC CTGGAGTGCC AAGGAGCCAT TCGCGTGGAT CAAGGTGGAT 
CTCCTGGCGC CCATGATTCT TCACGGCATC GAGACGCAGG GCGCCCGTCA CAGACTTTCC 
AGCCTTTATG TGTCTCAGTT CATCATCATG TACAGCCTGG ATGGGAAGCA GTGGCTGAGC 
TACCGAGGAA ACTCCACGGG AAGCCTGATG GTTTTCTTCG GCAACGTGGA CGCGTCCACT 
GTGAGGCACA ACCGCTTCGA CCCCCCGATC GTTGCCCGCT ACATCCGTGT GCACCCCACA 
CATGCCAGCA TCCGTACCGC CCTGCGCATG GAGCTGCTCG GCTGCGACCT GAACAGCTGC 
AGCGCGCCCC TGGGCATGGA GAGTGGATGG ATCTCGGACA CGCAGGTCAC TGCCTCGTCC 
CACCTCAGCA CCACGTTCTC TGCTTGGCCG CCTGCGCTCG CTCGGCTTCA CCTCCGGGGT 
GGGGCCAACG CCTGGCGACC CCAGGTGAAT GATCCCACAC AGTGGCTGCA GGTGGACTTG 
CAGAGGACTG TGAAAGTCAC CGGCGTCGTC ACCCAGGGCG CGCGGTCTCT CCTCACTGCC 
ATGTTTGTGA AAAAGTTCCT CGTCTCCACC AGCCAAGATG GCCGCCACTG GACTCACGTC 
TTGCAAGACG GCAAGGTCAA GGTTTTTCAG GGTAACCGGG ATGCGTCCAC GCCCATGGTG 
AACTCTCTGC ACCCCCCGCG CTTCACTCGA TACCTTCGGA TTCACCCCCA AGTGTGGGAG 
CGGCAAATTG CTCTGCGGCT CGAGATCCTG GGGTGTGAGG CTCAGCAGCT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899160.1
CDS	82..6858
Translation	MRAARGLCFFLSLCALCSCATRKYYLGAVELPWDYVSGGASGAQRSDSRFPPGTPAPSAHVVHTKTVFVEYMDRFFHTAKPRPLWMGLLGPTIWTEVHDTVVITLKNMASHPVSLHAVGMSFWKASEGAAYDDHSSPAEKDDDKVLPGESHTYAWQVLGESGPMASDPPCLTYAYLSHVDLVRDLNSGLVGALLVCKEGRLSAERTRMPPHFVLLFAVFDEGRSWHAATRDPASTEAQPTVHTVNGYTNRTLPGLTGCRGTSVYWHVMAVGTTPDIHSILLEGHTFSVRSHRQASLEISPMTLLTAQTWLMDLGRFLLFCHISSHRHGGMEAYVQVDNCPEAPQGQGKRDEDTEDYDDLDSEMDVFTWDPDAAPFVQVRSAAKRHPKTWVHYIAAEEGDWDYAPAAATLEDGSLRNRYLGRGPGRIGSKYKKVRFVAYTDGTFHTREATPREAGLLGPLLYGEVGDSLLIVFKNRASRAYNIHPHGIRDVGAVHAGRLPRGVKHVKDLPIRPGETFKYRWTLTAEDGPARSDARCVTRYYASAVDPERDLASGLIGPLLICCKESVDQRGNQMMSDERNVILFSVFDENRSWYISENMRRFLPDEAHVQLQDPEFEASNLMHSINGYVFDGLELRVCLHEVAAWHVLSVGAQTDFLSVFFSGHTFRHRAVYADTLTVFPHSGVTVFMSMDNPGVWVLGCHNPDFRESGMTALLKVSSCDEGVSDYYGETYEGVLAPLANDNAVDPRSFSQNSNHLHSRKKKSNTPMRFPLSHPTQHGSFWSDSQGDVHDAVHRAHSPAAAHSNEGPAEAAQLEPKPHPGEKMALPPSWLRAKRSLVTTMEAKRKKLDLQVQVSGLPDDRTTAVVAPDDPMAACKKAGSSGFPDESSPAALGKKMYPRIRPHGPLSIIEGNRDSNSSDSTLMYRLGSPPGDATSWTENRRGLGEKRSHRVAFLARGNTLLSDVDEKSHAPGPTSVGNSTAAVQDTILEICSEILEVTPPIHDRILSDKSATYLRPHRTPDRITSTERKDIRHEKDGDLVPQDADDTSAPFSEAPFLSESTDWLKEANGDNSAKPEQEPSPEQLVYLMYIKCMENQSFSSEKNKMIAGQGGFTKNTGLEDTVFPRKTSVFLTTVAKRQESGRHNQENIPQAVTEKEAPIEEKGALPQVHIATGSKNFPRDMFVLGTGQNINLHEETYVPVHKNVPWTRNPTDTRQIPMVHFFKKRKEEETNSGGLVNKTRETVRNYPSQKNSVARRREQASGRIKASARWLPDVNRSIQSLLKQIDHRKERKKFIIESRSADSSGTKSPTQTNHSPSHVVKMSAFPPRDIRRIESRDSSQVWVSSYAYDFETGSSRIRESSPFLNETETDNPSLAVPPRERFVRRGRFASPEKVNTHSAPCDKPENVVSSEPVSPGEAVTVALPPHVSTQEEEPLPAGSSCERRGHVDLFLEISLQRTRGPVERSRGRRSGGDTEGHTENPGKTPSPLPQMPKDQRGSEAGYPKICRWRDGTVLPPRPHNLSLGAKKKPNLPRREATRVEQGEAPSLVSPEPLVLRRRPREASTLLPGGEMREDDGDGVTAEYDDDDDVMAEYDDDAVTVDTPEDFDIYGEEAGQGPRGFQQKTRHYFIAAVEELWDYGVRVSPGALGDRAWSGDAARFRKVVFREFTDGSFTQRVHRGELDAHLGLLGPYIRAEVEDNIVVTFRNQASRPYSFYSSLVSYPEADGGAAPRSNFVRPNETKTYFWRVRPHMAPTDGEFDCKAWAYFSDVDLERDLHSGLIGPLLVCHASTLHPSHARPLAVQEFALLFAVFDETKSWYCAENLDRKCRPPRGTPAGDPAWREEYRFHAVNGYVADALLGLVMAEGRRTRWHLLSMGDPGHAQSVHFSAHSVTVRDGGEHRTAVCNLYPGVFTTVEMLPSRAGIWRVESLVGEHLRAGMSALFLVYSTRCQVPLGMASGYIRDSQITASGYHGLWTPQLARLHNAGSVNAWSAKEPFAWIKVDLLAPMILHGIETQGARHRLSSLYVSQFIIMYSLDGKQWLSYRGNSTGSLMVFFGNVDASTVRHNRFDPPIVARYIRVHPTHASIRTALRMELLGCDLNSCSAPLGMESGWISDTQVTASSHLSTTFSAWPPALARLHLRGGANAWRPQVNDPTQWLQVDLQRTVKVTGVVTQGARSLLTAMFVKKFLVSTSQDGRHWTHVLQDGKVKVFQGNRDASTPMVNSLHPPRFTRYLRIHPQVWERQIALRLEILGCEAQQLD

Target ORF information:

RefSeq Version	NM_183331.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus coagulation factor VIII (F8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183331.1

ORF Insert Sequence:

ATGCGCGCGG CGCGCGGCCT GTGCTTCTTC CTCAGCCTCT GCGCGCTCTG CTCCTGCGCC 
ACCCGGAAGT ACTACCTGGG CGCCGTGGAG TTGCCATGGG ACTATGTGAG CGGTGGTGCT 
TCCGGTGCCC AGCGCAGCGA CTCCAGGTTC CCTCCCGGGA CACCAGCGCC GTCTGCGCAT 
GTTGTGCACA CGAAGACGGT GTTTGTGGAG TACATGGACC GGTTTTTCCA CACGGCAAAG 
CCCAGGCCAC TGTGGATGGG TCTCCTAGGC CCCACCATTT GGACCGAGGT TCACGACACT 
GTGGTCATCA CACTCAAAAA CATGGCTTCT CATCCCGTCA GTCTCCACGC TGTCGGTATG 
TCCTTCTGGA AGGCGTCCGA GGGCGCGGCA TACGACGACC ACAGCAGCCC GGCCGAGAAG 
GACGACGACA AAGTCCTCCC CGGCGAGAGC CACACTTACG CGTGGCAGGT CCTGGGGGAG 
AGCGGCCCAA TGGCTTCCGA CCCCCCGTGC CTCACCTATG CCTACCTGTC TCATGTGGAC 
CTCGTGAGGG ACCTGAACTC GGGCCTCGTC GGGGCCCTGC TCGTGTGCAA AGAAGGCCGT 
CTGTCCGCAG AGAGAACACG GATGCCACCC CACTTCGTGC TGCTTTTTGC TGTCTTCGAT 
GAAGGGCGGA GCTGGCACGC AGCAACACGT GACCCCGCGT CCACCGAGGC CCAGCCCACG 
GTGCACACTG TGAATGGCTA CACGAACCGG ACACTGCCGG GTCTGACCGG ATGCCGCGGA 
ACATCCGTGT ACTGGCACGT GATGGCGGTG GGCACCACGC CCGACATACA CTCCATCCTC 
CTCGAAGGCC ACACATTCTC TGTGAGGAGC CACCGTCAGG CTTCCCTGGA GATCTCACCT 
ATGACTCTGC TCACGGCTCA GACGTGGCTG ATGGACCTGG GGCGGTTCCT GCTCTTCTGT 
CACATCTCCT CCCACAGACA CGGTGGCATG GAAGCCTACG TCCAGGTGGA CAACTGCCCC 
GAGGCGCCCC AGGGGCAGGG GAAAAGGGAC GAGGACACAG AGGACTATGA TGATCTGGAC 
TCGGAAATGG ACGTCTTCAC CTGGGACCCC GATGCCGCCC CCTTCGTCCA GGTCCGCTCA 
GCAGCCAAGA GACACCCCAA GACCTGGGTG CACTACATCG CAGCAGAGGA GGGGGACTGG 
GACTACGCGC CTGCGGCCGC CACCCTAGAA GACGGGAGCC TGCGGAACCG GTACCTGGGG 
CGCGGCCCTG GCCGCATCGG CAGCAAATAT AAGAAAGTCA GGTTCGTGGC CTACACGGAC 
GGCACCTTTC ACACGCGTGA GGCCACCCCG CGTGAAGCTG GGCTCCTAGG GCCACTGCTC 
TACGGGGAGG TCGGAGACTC ACTGTTGATT GTGTTCAAGA ACCGAGCGAG CCGGGCATAC 
AACATCCACC CTCATGGGAT CCGTGACGTT GGCGCTGTGC ATGCAGGGAG GCTGCCACGA 
GGGGTGAAGC ACGTGAAGGA TTTGCCGATC CGCCCAGGAG AGACGTTCAA GTACAGGTGG 
ACGCTGACCG CAGAGGATGG GCCTGCAAGG TCGGATGCGC GGTGTGTGAC GCGCTACTAT 
GCGAGCGCTG TGGATCCCGA GAGAGACCTG GCTTCCGGGC TGATTGGCCC CCTCCTCATC 
TGCTGTAAAG AATCTGTAGA TCAAAGGGGG AACCAGATGA TGTCAGACGA GAGGAACGTC 
ATCCTGTTCT CCGTGTTTGA TGAGAACCGG AGCTGGTACA TCTCGGAGAA CATGCGACGC 
TTCCTCCCCG ACGAGGCACA TGTGCAGCTG CAGGACCCCG AGTTCGAGGC CTCCAACCTC 
ATGCACAGCA TTAATGGCTA CGTCTTCGAT GGCCTGGAGC TGCGCGTTTG CCTGCACGAG 
GTGGCTGCCT GGCACGTGCT CAGCGTCGGC GCGCAGACTG ACTTCCTGTC TGTCTTCTTC 
TCTGGACACA CGTTCAGACA CAGGGCGGTG TACGCAGACA CACTCACGGT GTTCCCGCAC 
TCGGGGGTCA CCGTCTTCAT GTCCATGGAT AACCCAGGTG TGTGGGTGCT GGGGTGCCAC 
AACCCGGACT TCCGGGAGAG CGGGATGACC GCACTGCTGA AGGTTTCCAG TTGTGACGAG 
GGCGTGAGTG ATTACTACGG AGAGACCTAT GAGGGCGTTC TGGCCCCGTT GGCCAATGAC 
AACGCCGTGG ACCCCAGGAG CTTCTCCCAG AATTCAAATC ATCTGCACAG CAGGAAAAAG 
AAAAGCAACA CGCCGATGCG CTTCCCACTG AGCCATCCTA CCCAGCATGG CTCCTTTTGG 
TCGGACAGCC AGGGAGATGT CCATGACGCT GTTCATAGAG CCCATTCACC CGCTGCAGCA 
CACAGCAATG AAGGCCCAGC CGAAGCAGCC CAGCTCGAGC CCAAACCCCA TCCCGGTGAG 
AAAATGGCAC TTCCTCCTTC TTGGTTAAGA GCAAAGAGAA GTCTGGTGAC AACCATGGAA 
GCCAAGCGGA AGAAACTGGA TCTCCAGGTC CAGGTTTCTG GTTTGCCCGA CGACAGAACA 
ACCGCAGTGG TTGCGCCGGA CGATCCGATG GCGGCTTGTA AGAAGGCGGG TTCTTCAGGG 
TTTCCAGATG AATCGAGTCC CGCTGCATTG GGTAAGAAAA TGTATCCCCG TATCAGGCCC 
CACGGACCTT TAAGCATCAT TGAAGGAAAC AGGGATTCCA ACTCGTCGGA CTCGACTTTA 
ATGTATAGGC TGGGAAGTCC ACCAGGAGAC GCCACATCAT GGACGGAGAA CCGCAGAGGA 
CTTGGCGAGA AGAGGTCTCA CAGGGTCGCT TTCCTGGCCA GAGGTAACAC TTTACTCAGC 
GACGTTGATG AAAAATCGCA TGCCCCCGGC CCAACATCAG TTGGGAACAG CACAGCAGCC 
GTGCAGGACA CCATATTAGA GATCTGCAGT GAGATTCTCG AGGTCACACC TCCGATTCAT 
GACAGGATAC TTTCAGACAA AAGTGCTACA TATTTGAGAC CGCACCGTAC GCCCGACAGA 
ATCACCTCGA CGGAAAGGAA AGACATACGT CACGAAAAAG ATGGAGATCT TGTTCCGCAG 
GATGCAGATG ACACAAGCGC ACCATTCTCG GAGGCGCCTT TCCTGTCAGA ATCCACAGAT 
TGGTTAAAAG AGGCCAATGG GGATAACTCC GCAAAACCTG AGCAAGAACC CAGTCCAGAG 
CAATTAGTGT ATTTAATGTA TATAAAGTGT ATGGAAAATC AGAGTTTCTC GTCAGAGAAG 
AATAAAATGA TAGCAGGGCA AGGTGGATTT ACCAAGAACA CAGGACTTGA AGACACGGTT 
TTTCCCAGAA AGACGAGTGT GTTTCTCACC ACTGTGGCTA AGCGGCAAGA AAGTGGGAGG 
CACAACCAAG AAAACATTCC TCAGGCAGTG ACAGAGAAGG AAGCGCCAAT CGAAGAGAAG 
GGAGCTCTGC CCCAGGTGCA CATAGCGACT GGCTCTAAGA ACTTCCCGAG AGACATGTTT 
GTACTCGGTA CTGGGCAAAA TATAAATCTC CACGAAGAGA CATATGTACC AGTTCACAAA 
AACGTCCCAT GGACAAGAAA CCCAACAGAC ACAAGACAGA TTCCCATGGT TCATTTCTTT 
AAGAAAAGGA AGGAGGAGGA AACCAATTCA GGAGGCTTGG TAAATAAAAC GAGAGAAACG 
GTGAGAAACT ACCCAAGCCA GAAGAATAGC GTTGCTCGAC GTAGGGAACA GGCTTCGGGA 
CGAATCAAAG CATCCGCTCG TTGGCTTCCA GACGTAAACC GTTCAATCCA GAGTCTCCTT 
AAACAGATTG ACCACCGCAA GGAAAGGAAA AAGTTCATTA TAGAATCTCG CTCCGCAGAT 
TCTTCTGGGA CTAAAAGTCC CACTCAGACC AATCATTCTC CCTCACACGT TGTGAAAATG 
TCAGCATTCC CACCGAGAGA CATCCGAAGG ATCGAGTCGC GAGACAGTTC TCAGGTCTGG 
GTGTCGTCCT ATGCTTACGA CTTTGAGACA GGAAGTTCTA GAATTCGAGA GAGCAGTCCT 
TTCCTGAATG AAACTGAAAC GGATAACCCT TCTCTCGCCG TGCCACCGCG GGAGAGGTTC 
GTGCGTCGAG GAAGGTTCGC CTCCCCAGAA AAAGTGAACA CACACTCAGC GCCGTGTGAC 
AAACCCGAAA ATGTTGTCTC CTCGGAACCA GTGTCGCCTG GCGAAGCTGT CACAGTCGCA 
CTCCCTCCTC ACGTCTCCAC TCAGGAGGAG GAACCTTTGC CTGCAGGAAG TAGCTGTGAG 
CGTCGCGGAC ATGTGGATCT CTTTCTCGAG ATCTCTCTCC AGAGAACACG GGGGCCCGTT 
GAACGGAGTA GAGGAAGGAG GTCTGGGGGA GACACGGAAG GTCACACAGA GAACCCCGGA 
AAGACTCCCT CCCCACTGCC ACAGATGCCG AAAGATCAGC GTGGGTCTGA AGCGGGGTAC 
CCGAAAATCT GCCGCTGGAG AGACGGCACT GTTTTGCCCC CGAGGCCTCA CAACCTTTCC 
CTAGGAGCAA AGAAAAAACC AAATCTGCCC CGAAGAGAAG CCACTCGGGT GGAGCAAGGC 
GAGGCTCCGA GCCTGGTCTC TCCAGAGCCC CTGGTGCTGA GACGACGTCC AAGGGAAGCT 
AGCACCCTTC TGCCAGGAGG AGAGATGCGA GAGGACGACG GTGATGGTGT CACGGCGGAG 
TACGACGACG ACGATGACGT CATGGCAGAG TATGACGACG ATGCTGTCAC GGTGGACACG 
CCCGAGGATT TCGACATCTA TGGCGAGGAG GCAGGGCAGG GACCCCGCGG CTTTCAGCAG 
AAAACGCGGC ACTATTTCAT CGCGGCCGTG GAGGAGCTTT GGGACTACGG GGTGCGTGTG 
TCGCCTGGAG CTCTGGGAGA CAGGGCGTGG AGTGGGGACG CAGCTCGGTT CCGGAAAGTG 
GTTTTCCGTG AGTTCACGGA CGGGTCGTTC ACGCAGCGTG TGCACCGTGG GGAGCTGGAC 
GCACACCTGG GGCTATTGGG GCCATACATA CGGGCGGAAG TGGAGGACAA CATCGTGGTG 
ACCTTCCGGA ACCAGGCCTC CCGCCCCTAC TCCTTCTACT CCAGCCTCGT GTCCTACCCG 
GAAGCCGACG GTGGAGCTGC ACCAAGGAGC AACTTTGTGC GGCCCAACGA GACCAAAACT 
TACTTCTGGA GAGTCCGGCC CCACATGGCG CCCACGGACG GCGAGTTTGA CTGCAAGGCC 
TGGGCCTATT TCTCCGACGT GGACCTCGAG CGAGACCTGC ACTCGGGGCT GATTGGGCCA 
CTGCTCGTGT GCCACGCCTC CACCCTGCAC CCCTCGCACG CGCGGCCGCT CGCAGTGCAG 
GAGTTCGCGC TGCTCTTCGC GGTGTTCGAT GAGACCAAGA GCTGGTACTG CGCTGAGAAC 
CTGGACCGGA AGTGCAGGCC GCCCCGTGGC ACCCCCGCGG GAGACCCCGC CTGGAGAGAA 
GAGTACCGCT TCCATGCCGT GAATGGTTAC GTCGCAGATG CTCTGCTTGG CCTGGTGATG 
GCGGAAGGTC GGAGGACGAG GTGGCATCTG CTCAGCATGG GCGACCCCGG GCACGCACAG 
TCCGTGCATT TCAGCGCGCA CAGCGTCACC GTGCGGGACG GGGGCGAGCA CAGGACGGCG 
GTGTGCAACC TGTACCCAGG CGTGTTCACA ACCGTGGAGA TGTTGCCGTC CAGAGCGGGG 
ATCTGGCGGG TGGAGAGCCT GGTGGGCGAG CACCTACGGG CTGGGATGAG CGCCCTCTTC 
CTGGTGTACA GCACGCGCTG CCAGGTCCCG CTCGGCATGG CTTCCGGTTA CATCCGGGAC 
TCGCAGATCA CGGCTTCCGG CTACCACGGG CTGTGGACCC CGCAGCTCGC GAGGCTTCAC 
AACGCGGGAT CCGTGAATGC CTGGAGTGCC AAGGAGCCAT TCGCGTGGAT CAAGGTGGAT 
CTCCTGGCGC CCATGATTCT TCACGGCATC GAGACGCAGG GCGCCCGTCA CAGACTTTCC 
AGCCTTTATG TGTCTCAGTT CATCATCATG TACAGCCTGG ATGGGAAGCA GTGGCTGAGC 
TACCGAGGAA ACTCCACGGG AAGCCTGATG GTTTTCTTCG GCAACGTGGA CGCGTCCACT 
GTGAGGCACA ACCGCTTCGA CCCCCCGATC GTTGCCCGCT ACATCCGTGT GCACCCCACA 
CATGCCAGCA TCCGTACCGC CCTGCGCATG GAGCTGCTCG GCTGCGACCT GAACAGCTGC 
AGCGCGCCCC TGGGCATGGA GAGTGGATGG ATCTCGGACA CGCAGGTCAC TGCCTCGTCC 
CACCTCAGCA CCACGTTCTC TGCTTGGCCG CCTGCGCTCG CTCGGCTTCA CCTCCGGGGT 
GGGGCCAACG CCTGGCGACC CCAGGTGAAT GATCCCACAC AGTGGCTGCA GGTGGACTTG 
CAGAGGACTG TGAAAGTCAC CGGCGTCGTC ACCCAGGGCG CGCGGTCTCT CCTCACTGCC 
ATGTTTGTGA AAAAGTTCCT CGTCTCCACC AGCCAAGATG GCCGCCACTG GACTCACGTC 
TTGCAAGACG GCAAGGTCAA GGTTTTTCAG GGTAACCGGG ATGCGTCCAC GCCCATGGTG 
AACTCTCTGC ACCCCCCGCG CTTCACTCGA TACCTTCGGA TTCACCCCCA AGTGTGGGAG 
CGGCAAATTG CTCTGCGGCT CGAGATCCTG GGGTGTGAGG CTCAGCAGCT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Altered collagen turnover in factor VIII-deficient rats with hemophilic arthropathy identifies potential novel serological biomarkers in hemophilia.
Journal of thrombosis and haemostasis : JTH14(12)2419-2429(2016 Dec)
Manon-Jensen T,Karsdal MA,Nielsen LN,Kjelgaard-Hansen M,Vandahl B,Olsen EH,Enoksson M,Roepstorff K

How it all starts: Initiation of the clotting cascade.
Critical reviews in biochemistry and molecular biology50(4)326-36(2015)
Smith SA,Travers RJ,Morrissey JH

A novel F8 -/- rat as a translational model of human hemophilia A.
Journal of thrombosis and haemostasis : JTH12(8)1274-82(2014 Aug)
Nielsen LN,Wiinberg B,H?ger M,Holmberg HL,Hansen JJ,Roepstorff K,Tranholm M

WAG-F8(m1Ycb) rats harboring a factor VIII gene mutation provide a new animal model for hemophilia A.
Journal of thrombosis and haemostasis : JTH8(11)2472-7(2010 Nov)
Booth CJ,Brooks MB,Rockwell S,Murphy JW,Rinder HM,Zelterman D,Paidas MJ,Compton SR,Marks PW

Detection of 95 novel mutations in coagulation factor VIII gene F8 responsible for hemophilia A: results from a single institution.
Human mutation27(7)676-85(2006 Jul)
Guillet B,Lambert T,d'Oiron R,Proulle V,Plantier JL,Rafowicz A,Peynet J,Costa JM,Bendelac L,Laurian Y,Lavergne JM

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F8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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