NOLC1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOLC1
Entrez Gene ID	9221
Full Name	nucleolar and coiled-body phosphoprotein 1
Synonyms	NOPP130,NOPP140,NS5ATP13,P130
General protein information	Preferred Names nucleolar and coiled-body phosphoprotein 1 Names nucleolar and coiled-body phosphoprotein 1 140 kDa nucleolar phosphoprotein HCV NS5A trans-regulated protein 13 HCV NS5A-transactivated protein 13 hepatitis C virus NS5A-transactivated protein 13 nucleolar 130 kDa protein nucleolar and coiled-body phosphprotein 1 nucleolar phosphoprotein p130 nucleolar protein p130
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q24.32
Disorder MIM:	602394

mRNA and Protein(s)

mRNA	Protein	Name
NM_004741.4	NP_004732.2	nucleolar and coiled-body phosphoprotein 1 isoform 2
XM_005270273.1	XP_005270330.1	nucleolar and coiled-body phosphoprotein 1 isoform X1
NM_001284389.1	NP_001271318.1	nucleolar and coiled-body phosphoprotein 1 isoform 3
NM_004741.5	NP_004732.2	nucleolar and coiled-body phosphoprotein 1 isoform 2
XM_005270273.2	XP_005270330.1	nucleolar and coiled-body phosphoprotein 1 isoform X1
NM_001284389.2	NP_001271318.1	nucleolar and coiled-body phosphoprotein 1 isoform 3
NM_001284388.1	NP_001271317.1	nucleolar and coiled-body phosphoprotein 1 isoform 1

Pathways from BioSystems

Homologies

	LOC712789	XP_001112196.2
Bos taurus (cattle)	NOLC1	NP_001069076.1
Pan troglodytes (chimpanzee)	NOLC1	XP_001171017.1
Canis lupus familiaris (dog)	NOLC1	XP_005637707.1
Homo sapiens (human)	NOLC1	NP_001271318.1
Rattus norvegicus (Norway rat)	Nolc1	NP_074060.1

Gene Ontology
Bibliography

Related articles in PubMed

Biophysical characterization of the structural change of Nopp140, an intrinsically disordered protein, in the interaction with CK2?.
Na JH, Lee WK, Kim Y, Jeong C, Song SS, Cha SS, Han KH, Shin YK, Yu YG
Biochemical and biophysical research communications477(2)181-7(2016 08)

Cell-fate determination by ubiquitin-dependent regulation of translation.
Werner A, Iwasaki S, McGourty CA, Medina-Ruiz S, Teerikorpi N, Fedrigo I, Ingolia NT, Rape M
Nature525(7570)523-7(2015 Sep)

Identification of NS1 domains of avian H5N1 influenza virus which influence the interaction with the NOLC1 protein.
Zhu CY, Zheng FL, She XS, Zhao D, Gu Y, Duan YT, Chang AK, Liu HS
Virus genes50(2)238-44(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In this study, the disordered feature of Nopp140 and the effect of CK2alpha on the structure of Nopp140 were examined using single-molecule fluorescence resonance energy transfer (smFRET) and electron paramagnetic resonance (EPR).
Title: Biophysical characterization of the structural change of Nopp140, an intrinsically disordered protein, in the interaction with CK2?.

pRb2/p130 cytoplasmic delocalization can lead to cell cycle deregulation
Title: pRb2/p130 localizes to the cytoplasm in diffuse gastric cancer.

D120 and R195 of H5N1 influenza virus NS1 were crucial for the binding to host NOLC1.
Title: Identification of NS1 domains of avian H5N1 influenza virus which influence the interaction with the NOLC1 protein.

our study demonstrated that DNA methylation is a key mechanism of silenced NOLC1 expression in human hepatocellular carcinoma cells, and NOLC1 gene hypermethylation of the four CpG dinucleotides is a potential biomarker for hepatocellular carcinoma.
Title: Methylation of nucleolar and coiled-body phosphoprotein?1 is associated with the mechanism of tumorigenesis in hepatocellular carcinoma.

A Nopp140 fragment (residues 568-596) and inositol hexakisphosphate competitively bind to the catalytic subunit of CK2 (CK2alpha), and phospho-Ser574 of Nopp140 significantly enhances its interaction with CK2alpha.
Title: Structural and functional insights into the regulation mechanism of CK2 by IP6 and the intrinsically disordered protein Nopp140.

The following NOLC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOLC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15724	NM_001284389.2 Latest version!	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu15982	NM_001284388.1 Latest version!	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu15808	NM_004741.5 Latest version!	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$496.30 $709.00
OHu44669	XM_005270273.2 Latest version!	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu15808	NM_004741.4	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$496.30 $709.00
OHu44669	XM_005270273.1	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu15724	NM_001284389.1	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOLC1 ( NM_001284389.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271318.1 Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu15724
Clone ID Related Accession (Same CDS sequence)	NM_001284389.1 , NM_001284389.2
Accession Version	NM_001284389.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	nucleolar and coiled-body phosphoprotein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA588285.1, BC001883.1, D21262.1, AL520884.3 and CF145090.1. On Oct 1, 2013 this sequence version replaced XM_005270275.1. Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.135394.1, SRR1803613.160354.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAACAGG 
TCTGCCAAGG TCCCAGAGCG AAAGTTACAG GCAAATGGAC CAGTGGCTAA GAAAGCTAAG 
AAGAAGGCCT CATCCAGTGA CAGTGAGGAC AGCAGCGAGG AGGAGGAGGA AGTTCAAGGG 
CCTCCAGCAA AGAAGGCTGC TGTACCTGCC AAGCGAGTCG GTCTGCCTCC TGGGAAGGCT 
GCAGCCAAAG CATCAGAGAG TAGCAGCAGT GAAGAGTCCA GTGATGATGA TGATGAGGAG 
GACCAAAAGA AACAGCCTGT CCAGAAGGGA GTTAAGCCCC AAGCCAAGGC AGCCAAAGCT 
CCTCCTAAGA AGGCCAAGAG CTCTGATTCT GATTCTGACT CAAGCTCCGA GGATGAGCCA 
CCAAAGAACC AGAAGCCAAA GATAACACCT GTGACAGTTA AAGCTCAGAC TAAAGCCCCT 
CCCAAACCAG CTCGAGCAGC ACCTAAAATA GCCAATGGTA AAGCAGCCAG TAGCAGCAGT 
AGCAGCAGCA GCAGCAGTAG CAGTGATGAC TCAGAGGAGG AGAAGGCAGC AGCCACCCCC 
AAGAAGACTG TACCTAAAAA GCAAGTTGTG GCCAAGGCCC CAGTGAAAGC AGCTACCACC 
CCTACCCGGA AGAGTTCTAG CAGTGAGGAT TCCTCCAGTG ACGAGGAAGA GGAGCAAAAA 
AAACCCATGA AAAATAAACC AGGTCCCTAC AGTTCAGTCC CCCCGCCTTC TGCTCCCCCA 
CCAAAGAAGT CTCTGGGAAC CCAGCCTCCC AAGAAGGCTG TGGAGAAGCA GCAGCCTGTG 
GAAAGCAGTG AAGACAGCAG TGATGAGTCT GATTCAAGTT CTGAAGAAGA GAAGAAACCC 
CCAACTAAGG CAGTAGTCTC TAAAGCAACC ACTAAACCAC CTCCAGCAAA GAAAGCAGCA 
GAGAGCTCTT CAGACAGCTC AGACTCTGAC AGCTCTGAGG ATGATGAAGC TCCTTCTAAG 
CCAGCTGGTA CCACCAAGAA TTCTTCAAAT AAGCCAGCTG TCACCACCAA GTCACCTGCA 
GTGAAGCCAG CTGCAGCCCC CAAGCAACCT GTGGGCGGTG GCCAGAAGCT TCTGACGAGA 
AAGGCTGACA GCAGCTCCAG TGAGGAAGAG AGCAGCTCCA GTGAGGAGGA GAAGACAAAG 
AAGATGGTGG CCACCACTAA GCCCAAGGCG ACTGCCAAAG CAGCTCTATC TCTGCCTGCC 
AAGCAGGCTC CTCAGGGTAG TAGGGACAGC AGCTCTGATT CAGACAGCTC CAGCAGTGAG 
GAGGAGGAAG AGAAGACATC TAAGTCTGCA GTTAAGAAGA AGCCACAGAA GGTAGCAGGA 
GGTGCAGCCC CTTCCAAGCC AGCCTCTGCA AAGAAAGGAA AGGCTGAGAG CAGCAACAGT 
TCTTCTTCTG ATGACTCCAG TGAGGAAGAG GAAGAGAAGC TCAAGGGCAA GGGCTCTCCA 
AGACCACAAG CCCCCAAGGC CAATGGCACC TCTGCACTGA CTGCCCAGAA TGGAAAAGCA 
GCTAAGAACA GTGAGGAGGA GGAAGAAGAA AAGAAAAAGG CGGCAGTGGT AGTTTCCAAA 
TCAGGTTCAT TAAAGAAGCG GAAGCAGAAT GAGGCTGCCA AGGAGGCAGA GACTCCTCAG 
GCCAAGAAGA TAAAGCTTCA GACCCCTAAC ACATTTCCAA AAAGGAAGAA AGGAGAAAAA 
AGGGCATCAT CCCCATTCCG AAGGGTCAGG GAGGAGGAAA TTGAGGTGGA TTCACGAGTT 
GCGGACAACT CCTTTGATGC CAAGCGAGGT GCAGCCGGAG ACTGGGGAGA GCGAGCCAAT 
CAGGTTTTGA AGTTCACCAA AGGCAAGTCC TTTCGGCATG AGAAAACCAA GAAGAAGCGG 
GGCAGCTACC GGGGAGGCTC AATCTCTGTC CAGGTCAATT CTATTAAGTT TGACAGCGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271318.1
CDS	236..2338
Translation	MADAGIRRVVPSDLYPLVLGFLRDNQLSEVANKFAKATGATQQDANASSLLDIYSFWLNRSAKVPERKLQANGPVAKKAKKKASSSDSEDSSEEEEEVQGPPAKKAAVPAKRVGLPPGKAAAKASESSSSEESSDDDDEEDQKKQPVQKGVKPQAKAAKAPPKKAKSSDSDSDSSSEDEPPKNQKPKITPVTVKAQTKAPPKPARAAPKIANGKAASSSSSSSSSSSSDDSEEEKAAATPKKTVPKKQVVAKAPVKAATTPTRKSSSSEDSSSDEEEEQKKPMKNKPGPYSSVPPPSAPPPKKSLGTQPPKKAVEKQQPVESSEDSSDESDSSSEEEKKPPTKAVVSKATTKPPPAKKAAESSSDSSDSDSSEDDEAPSKPAGTTKNSSNKPAVTTKSPAVKPAAAPKQPVGGGQKLLTRKADSSSSEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSRDSSSDSDSSSSEEEEEKTSKSAVKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKGSPRPQAPKANGTSALTAQNGKAAKNSEEEEEEKKKAAVVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFDSE

Target ORF information:

RefSeq Version	NM_001284389.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284389.1

ORF Insert Sequence:

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAACAGG 
TCTGCCAAGG TCCCAGAGCG AAAGTTACAG GCAAATGGAC CAGTGGCTAA GAAAGCTAAG 
AAGAAGGCCT CATCCAGTGA CAGTGAGGAC AGCAGCGAGG AGGAGGAGGA AGTTCAAGGG 
CCTCCAGCAA AGAAGGCTGC TGTACCTGCC AAGCGAGTCG GTCTGCCTCC TGGGAAGGCT 
GCAGCCAAAG CATCAGAGAG TAGCAGCAGT GAAGAGTCCA GTGATGATGA TGATGAGGAG 
GACCAAAAGA AACAGCCTGT CCAGAAGGGA GTTAAGCCCC AAGCCAAGGC AGCCAAAGCT 
CCTCCTAAGA AGGCCAAGAG CTCTGATTCT GATTCTGACT CAAGCTCCGA GGATGAGCCA 
CCAAAGAACC AGAAGCCAAA GATAACACCT GTGACAGTTA AAGCTCAGAC TAAAGCCCCT 
CCCAAACCAG CTCGAGCAGC ACCTAAAATA GCCAATGGTA AAGCAGCCAG TAGCAGCAGT 
AGCAGCAGCA GCAGCAGTAG CAGTGATGAC TCAGAGGAGG AGAAGGCAGC AGCCACCCCC 
AAGAAGACTG TACCTAAAAA GCAAGTTGTG GCCAAGGCCC CAGTGAAAGC AGCTACCACC 
CCTACCCGGA AGAGTTCTAG CAGTGAGGAT TCCTCCAGTG ACGAGGAAGA GGAGCAAAAA 
AAACCCATGA AAAATAAACC AGGTCCCTAC AGTTCAGTCC CCCCGCCTTC TGCTCCCCCA 
CCAAAGAAGT CTCTGGGAAC CCAGCCTCCC AAGAAGGCTG TGGAGAAGCA GCAGCCTGTG 
GAAAGCAGTG AAGACAGCAG TGATGAGTCT GATTCAAGTT CTGAAGAAGA GAAGAAACCC 
CCAACTAAGG CAGTAGTCTC TAAAGCAACC ACTAAACCAC CTCCAGCAAA GAAAGCAGCA 
GAGAGCTCTT CAGACAGCTC AGACTCTGAC AGCTCTGAGG ATGATGAAGC TCCTTCTAAG 
CCAGCTGGTA CCACCAAGAA TTCTTCAAAT AAGCCAGCTG TCACCACCAA GTCACCTGCA 
GTGAAGCCAG CTGCAGCCCC CAAGCAACCT GTGGGCGGTG GCCAGAAGCT TCTGACGAGA 
AAGGCTGACA GCAGCTCCAG TGAGGAAGAG AGCAGCTCCA GTGAGGAGGA GAAGACAAAG 
AAGATGGTGG CCACCACTAA GCCCAAGGCG ACTGCCAAAG CAGCTCTATC TCTGCCTGCC 
AAGCAGGCTC CTCAGGGTAG TAGGGACAGC AGCTCTGATT CAGACAGCTC CAGCAGTGAG 
GAGGAGGAAG AGAAGACATC TAAGTCTGCA GTTAAGAAGA AGCCACAGAA GGTAGCAGGA 
GGTGCAGCCC CTTCCAAGCC AGCCTCTGCA AAGAAAGGAA AGGCTGAGAG CAGCAACAGT 
TCTTCTTCTG ATGACTCCAG TGAGGAAGAG GAAGAGAAGC TCAAGGGCAA GGGCTCTCCA 
AGACCACAAG CCCCCAAGGC CAATGGCACC TCTGCACTGA CTGCCCAGAA TGGAAAAGCA 
GCTAAGAACA GTGAGGAGGA GGAAGAAGAA AAGAAAAAGG CGGCAGTGGT AGTTTCCAAA 
TCAGGTTCAT TAAAGAAGCG GAAGCAGAAT GAGGCTGCCA AGGAGGCAGA GACTCCTCAG 
GCCAAGAAGA TAAAGCTTCA GACCCCTAAC ACATTTCCAA AAAGGAAGAA AGGAGAAAAA 
AGGGCATCAT CCCCATTCCG AAGGGTCAGG GAGGAGGAAA TTGAGGTGGA TTCACGAGTT 
GCGGACAACT CCTTTGATGC CAAGCGAGGT GCAGCCGGAG ACTGGGGAGA GCGAGCCAAT 
CAGGTTTTGA AGTTCACCAA AGGCAAGTCC TTTCGGCATG AGAAAACCAA GAAGAAGCGG 
GGCAGCTACC GGGGAGGCTC AATCTCTGTC CAGGTCAATT CTATTAAGTT TGACAGCGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOLC1 ( NM_001284389.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271318.1 Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu15724
Clone ID Related Accession (Same CDS sequence)	NM_001284389.1 , NM_001284389.2
Accession Version	NM_001284389.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	nucleolar and coiled-body phosphoprotein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA588285.1, BC001883.1, D21262.1 and AL520884.3. On Jun 2, 2019 this sequence version replaced NM_001284389.1. Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.172386.1, BC001883.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAACAGG 
TCTGCCAAGG TCCCAGAGCG AAAGTTACAG GCAAATGGAC CAGTGGCTAA GAAAGCTAAG 
AAGAAGGCCT CATCCAGTGA CAGTGAGGAC AGCAGCGAGG AGGAGGAGGA AGTTCAAGGG 
CCTCCAGCAA AGAAGGCTGC TGTACCTGCC AAGCGAGTCG GTCTGCCTCC TGGGAAGGCT 
GCAGCCAAAG CATCAGAGAG TAGCAGCAGT GAAGAGTCCA GTGATGATGA TGATGAGGAG 
GACCAAAAGA AACAGCCTGT CCAGAAGGGA GTTAAGCCCC AAGCCAAGGC AGCCAAAGCT 
CCTCCTAAGA AGGCCAAGAG CTCTGATTCT GATTCTGACT CAAGCTCCGA GGATGAGCCA 
CCAAAGAACC AGAAGCCAAA GATAACACCT GTGACAGTTA AAGCTCAGAC TAAAGCCCCT 
CCCAAACCAG CTCGAGCAGC ACCTAAAATA GCCAATGGTA AAGCAGCCAG TAGCAGCAGT 
AGCAGCAGCA GCAGCAGTAG CAGTGATGAC TCAGAGGAGG AGAAGGCAGC AGCCACCCCC 
AAGAAGACTG TACCTAAAAA GCAAGTTGTG GCCAAGGCCC CAGTGAAAGC AGCTACCACC 
CCTACCCGGA AGAGTTCTAG CAGTGAGGAT TCCTCCAGTG ACGAGGAAGA GGAGCAAAAA 
AAACCCATGA AAAATAAACC AGGTCCCTAC AGTTCAGTCC CCCCGCCTTC TGCTCCCCCA 
CCAAAGAAGT CTCTGGGAAC CCAGCCTCCC AAGAAGGCTG TGGAGAAGCA GCAGCCTGTG 
GAAAGCAGTG AAGACAGCAG TGATGAGTCT GATTCAAGTT CTGAAGAAGA GAAGAAACCC 
CCAACTAAGG CAGTAGTCTC TAAAGCAACC ACTAAACCAC CTCCAGCAAA GAAAGCAGCA 
GAGAGCTCTT CAGACAGCTC AGACTCTGAC AGCTCTGAGG ATGATGAAGC TCCTTCTAAG 
CCAGCTGGTA CCACCAAGAA TTCTTCAAAT AAGCCAGCTG TCACCACCAA GTCACCTGCA 
GTGAAGCCAG CTGCAGCCCC CAAGCAACCT GTGGGCGGTG GCCAGAAGCT TCTGACGAGA 
AAGGCTGACA GCAGCTCCAG TGAGGAAGAG AGCAGCTCCA GTGAGGAGGA GAAGACAAAG 
AAGATGGTGG CCACCACTAA GCCCAAGGCG ACTGCCAAAG CAGCTCTATC TCTGCCTGCC 
AAGCAGGCTC CTCAGGGTAG TAGGGACAGC AGCTCTGATT CAGACAGCTC CAGCAGTGAG 
GAGGAGGAAG AGAAGACATC TAAGTCTGCA GTTAAGAAGA AGCCACAGAA GGTAGCAGGA 
GGTGCAGCCC CTTCCAAGCC AGCCTCTGCA AAGAAAGGAA AGGCTGAGAG CAGCAACAGT 
TCTTCTTCTG ATGACTCCAG TGAGGAAGAG GAAGAGAAGC TCAAGGGCAA GGGCTCTCCA 
AGACCACAAG CCCCCAAGGC CAATGGCACC TCTGCACTGA CTGCCCAGAA TGGAAAAGCA 
GCTAAGAACA GTGAGGAGGA GGAAGAAGAA AAGAAAAAGG CGGCAGTGGT AGTTTCCAAA 
TCAGGTTCAT TAAAGAAGCG GAAGCAGAAT GAGGCTGCCA AGGAGGCAGA GACTCCTCAG 
GCCAAGAAGA TAAAGCTTCA GACCCCTAAC ACATTTCCAA AAAGGAAGAA AGGAGAAAAA 
AGGGCATCAT CCCCATTCCG AAGGGTCAGG GAGGAGGAAA TTGAGGTGGA TTCACGAGTT 
GCGGACAACT CCTTTGATGC CAAGCGAGGT GCAGCCGGAG ACTGGGGAGA GCGAGCCAAT 
CAGGTTTTGA AGTTCACCAA AGGCAAGTCC TTTCGGCATG AGAAAACCAA GAAGAAGCGG 
GGCAGCTACC GGGGAGGCTC AATCTCTGTC CAGGTCAATT CTATTAAGTT TGACAGCGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271318.1
CDS	23..2125
Translation	MADAGIRRVVPSDLYPLVLGFLRDNQLSEVANKFAKATGATQQDANASSLLDIYSFWLNRSAKVPERKLQANGPVAKKAKKKASSSDSEDSSEEEEEVQGPPAKKAAVPAKRVGLPPGKAAAKASESSSSEESSDDDDEEDQKKQPVQKGVKPQAKAAKAPPKKAKSSDSDSDSSSEDEPPKNQKPKITPVTVKAQTKAPPKPARAAPKIANGKAASSSSSSSSSSSSDDSEEEKAAATPKKTVPKKQVVAKAPVKAATTPTRKSSSSEDSSSDEEEEQKKPMKNKPGPYSSVPPPSAPPPKKSLGTQPPKKAVEKQQPVESSEDSSDESDSSSEEEKKPPTKAVVSKATTKPPPAKKAAESSSDSSDSDSSEDDEAPSKPAGTTKNSSNKPAVTTKSPAVKPAAAPKQPVGGGQKLLTRKADSSSSEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSRDSSSDSDSSSSEEEEEKTSKSAVKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKGSPRPQAPKANGTSALTAQNGKAAKNSEEEEEEKKKAAVVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFDSE

Target ORF information:

RefSeq Version	NM_001284389.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284389.2

ORF Insert Sequence:

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAACAGG 
TCTGCCAAGG TCCCAGAGCG AAAGTTACAG GCAAATGGAC CAGTGGCTAA GAAAGCTAAG 
AAGAAGGCCT CATCCAGTGA CAGTGAGGAC AGCAGCGAGG AGGAGGAGGA AGTTCAAGGG 
CCTCCAGCAA AGAAGGCTGC TGTACCTGCC AAGCGAGTCG GTCTGCCTCC TGGGAAGGCT 
GCAGCCAAAG CATCAGAGAG TAGCAGCAGT GAAGAGTCCA GTGATGATGA TGATGAGGAG 
GACCAAAAGA AACAGCCTGT CCAGAAGGGA GTTAAGCCCC AAGCCAAGGC AGCCAAAGCT 
CCTCCTAAGA AGGCCAAGAG CTCTGATTCT GATTCTGACT CAAGCTCCGA GGATGAGCCA 
CCAAAGAACC AGAAGCCAAA GATAACACCT GTGACAGTTA AAGCTCAGAC TAAAGCCCCT 
CCCAAACCAG CTCGAGCAGC ACCTAAAATA GCCAATGGTA AAGCAGCCAG TAGCAGCAGT 
AGCAGCAGCA GCAGCAGTAG CAGTGATGAC TCAGAGGAGG AGAAGGCAGC AGCCACCCCC 
AAGAAGACTG TACCTAAAAA GCAAGTTGTG GCCAAGGCCC CAGTGAAAGC AGCTACCACC 
CCTACCCGGA AGAGTTCTAG CAGTGAGGAT TCCTCCAGTG ACGAGGAAGA GGAGCAAAAA 
AAACCCATGA AAAATAAACC AGGTCCCTAC AGTTCAGTCC CCCCGCCTTC TGCTCCCCCA 
CCAAAGAAGT CTCTGGGAAC CCAGCCTCCC AAGAAGGCTG TGGAGAAGCA GCAGCCTGTG 
GAAAGCAGTG AAGACAGCAG TGATGAGTCT GATTCAAGTT CTGAAGAAGA GAAGAAACCC 
CCAACTAAGG CAGTAGTCTC TAAAGCAACC ACTAAACCAC CTCCAGCAAA GAAAGCAGCA 
GAGAGCTCTT CAGACAGCTC AGACTCTGAC AGCTCTGAGG ATGATGAAGC TCCTTCTAAG 
CCAGCTGGTA CCACCAAGAA TTCTTCAAAT AAGCCAGCTG TCACCACCAA GTCACCTGCA 
GTGAAGCCAG CTGCAGCCCC CAAGCAACCT GTGGGCGGTG GCCAGAAGCT TCTGACGAGA 
AAGGCTGACA GCAGCTCCAG TGAGGAAGAG AGCAGCTCCA GTGAGGAGGA GAAGACAAAG 
AAGATGGTGG CCACCACTAA GCCCAAGGCG ACTGCCAAAG CAGCTCTATC TCTGCCTGCC 
AAGCAGGCTC CTCAGGGTAG TAGGGACAGC AGCTCTGATT CAGACAGCTC CAGCAGTGAG 
GAGGAGGAAG AGAAGACATC TAAGTCTGCA GTTAAGAAGA AGCCACAGAA GGTAGCAGGA 
GGTGCAGCCC CTTCCAAGCC AGCCTCTGCA AAGAAAGGAA AGGCTGAGAG CAGCAACAGT 
TCTTCTTCTG ATGACTCCAG TGAGGAAGAG GAAGAGAAGC TCAAGGGCAA GGGCTCTCCA 
AGACCACAAG CCCCCAAGGC CAATGGCACC TCTGCACTGA CTGCCCAGAA TGGAAAAGCA 
GCTAAGAACA GTGAGGAGGA GGAAGAAGAA AAGAAAAAGG CGGCAGTGGT AGTTTCCAAA 
TCAGGTTCAT TAAAGAAGCG GAAGCAGAAT GAGGCTGCCA AGGAGGCAGA GACTCCTCAG 
GCCAAGAAGA TAAAGCTTCA GACCCCTAAC ACATTTCCAA AAAGGAAGAA AGGAGAAAAA 
AGGGCATCAT CCCCATTCCG AAGGGTCAGG GAGGAGGAAA TTGAGGTGGA TTCACGAGTT 
GCGGACAACT CCTTTGATGC CAAGCGAGGT GCAGCCGGAG ACTGGGGAGA GCGAGCCAAT 
CAGGTTTTGA AGTTCACCAA AGGCAAGTCC TTTCGGCATG AGAAAACCAA GAAGAAGCGG 
GGCAGCTACC GGGGAGGCTC AATCTCTGTC CAGGTCAATT CTATTAAGTT TGACAGCGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOLC1 ( NM_001284388.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271317.1 Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 1, mRNA.

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CloneID	OHu15982
Clone ID Related Accession (Same CDS sequence)	NM_001284388.1
Accession Version	NM_001284388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	nucleolar and coiled-body phosphoprotein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA588285.1, D21262.1, AK314363.1, AL520884.3 and CF145090.1. On Oct 1, 2013 this sequence version replaced XM_005270274.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.122271.1, SRR1803616.232347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAAGTCT 
GCCAAGGTCC CAGAGCGAAA GTTACAGGCA AATGGACCAG TGGCTAAGAA AGCTAAGAAG 
AAGGCCTCAT CCAGTGACAG TGAGGACAGC AGCGAGGAGG AGGAGGAAGT TCAAGGGCCT 
CCAGCAAAGA AGGCTGCTGT ACCTGCCAAG CGAGTCGGTC TGCCTCCTGG GAAGGCTGCA 
GCCAAAGCAT CAGAGAGTAG CAGCAGTGAA GAGTCCAGTG ATGATGATGA TGAGGAGGAC 
CAAAAGAAAC AGCCTGTCCA GAAGGGAGTT AAGCCCCAAG CCAAGGCAGC CAAAGCTCCT 
CCTAAGAAGG CCAAGAGCTC TGATTCTGAT TCTGACTCAA GCTCCGAGGA TGAGCCACCA 
AAGAACCAGA AGCCAAAGAT AACACCTGTG ACAGTTAAAG CTCAGACTAA AGCCCCTCCC 
AAACCAGCTC GAGCAGCACC TAAAATAGCC AATGGTAAAG CAGCCAGTAG CAGCAGTAGC 
AGCAGCAGCA GCAGTAGCAG TGATGACTCA GAGGAGGAGA AGGCAGCAGC CACCCCCAAG 
AAGGTCTGGA CCATAACTTC TGTCAGGGCA GAGACTGTAC CTAAAAAGCA AGTTGTGGCC 
AAGGCCCCAG TGAAAGCAGC TACCACCCCT ACCCGGAAGA GTTCTAGCAG TGAGGATTCC 
TCCAGTGACG AGGAAGAGGA GCAAAAAAAA CCCATGAAAA ATAAACCAGG TCCCTACAGT 
TCAGTCCCCC CGCCTTCTGC TCCCCCACCA AAGAAGTCTC TGGGAACCCA GCCTCCCAAG 
AAGGCTGTGG AGAAGCAGCA GCCTGTGGAA AGCAGTGAAG ACAGCAGTGA TGAGTCTGAT 
TCAAGTTCTG AAGAAGAGAA GAAACCCCCA ACTAAGGCAG TAGTCTCTAA AGCAACCACT 
AAACCACCTC CAGCAAAGAA AGCAGCAGAG AGCTCTTCAG ACAGCTCAGA CTCTGACAGC 
TCTGAGGATG ATGAAGCTCC TTCTAAGCCA GCTGGTACCA CCAAGAATTC TTCAAATAAG 
CCAGCTGTCA CCACCAAGTC ACCTGCAGTG AAGCCAGCTG CAGCCCCCAA GCAACCTGTG 
GGCGGTGGCC AGAAGCTTCT GACGAGAAAG GCTGACAGCA GCTCCAGTGA GGAAGAGAGC 
AGCTCCAGTG AGGAGGAGAA GACAAAGAAG ATGGTGGCCA CCACTAAGCC CAAGGCGACT 
GCCAAAGCAG CTCTATCTCT GCCTGCCAAG CAGGCTCCTC AGGGTAGTAG GGACAGCAGC 
TCTGATTCAG ACAGCTCCAG CAGTGAGGAG GAGGAAGAGA AGACATCTAA GTCTGCAGTT 
AAGAAGAAGC CACAGAAGGT AGCAGGAGGT GCAGCCCCTT CCAAGCCAGC CTCTGCAAAG 
AAAGGAAAGG CTGAGAGCAG CAACAGTTCT TCTTCTGATG ACTCCAGTGA GGAAGAGGAA 
GAGAAGCTCA AGGGCAAGGG CTCTCCAAGA CCACAAGCCC CCAAGGCCAA TGGCACCTCT 
GCACTGACTG CCCAGAATGG AAAAGCAGCT AAGAACAGTG AGGAGGAGGA AGAAGAAAAG 
AAAAAGGCGG CAGTGGTAGT TTCCAAATCA GGTTCATTAA AGAAGCGGAA GCAGAATGAG 
GCTGCCAAGG AGGCAGAGAC TCCTCAGGCC AAGAAGATAA AGCTTCAGAC CCCTAACACA 
TTTCCAAAAA GGAAGAAAGG AGAAAAAAGG GCATCATCCC CATTCCGAAG GGTCAGGGAG 
GAGGAAATTG AGGTGGATTC ACGAGTTGCG GACAACTCCT TTGATGCCAA GCGAGGTGCA 
GCCGGAGACT GGGGAGAGCG AGCCAATCAG GTTTTGAAGT TCACCAAAGG CAAGTCCTTT 
CGGCATGAGA AAACCAAGAA GAAGCGGGGC AGCTACCGGG GAGGCTCAAT CTCTGTCCAG 
GTCAATTCTA TTAAGTTTGA CAGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271317.1
CDS	236..2365
Translation	MADAGIRRVVPSDLYPLVLGFLRDNQLSEVANKFAKATGATQQDANASSLLDIYSFWLKSAKVPERKLQANGPVAKKAKKKASSSDSEDSSEEEEEVQGPPAKKAAVPAKRVGLPPGKAAAKASESSSSEESSDDDDEEDQKKQPVQKGVKPQAKAAKAPPKKAKSSDSDSDSSSEDEPPKNQKPKITPVTVKAQTKAPPKPARAAPKIANGKAASSSSSSSSSSSSDDSEEEKAAATPKKVWTITSVRAETVPKKQVVAKAPVKAATTPTRKSSSSEDSSSDEEEEQKKPMKNKPGPYSSVPPPSAPPPKKSLGTQPPKKAVEKQQPVESSEDSSDESDSSSEEEKKPPTKAVVSKATTKPPPAKKAAESSSDSSDSDSSEDDEAPSKPAGTTKNSSNKPAVTTKSPAVKPAAAPKQPVGGGQKLLTRKADSSSSEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSRDSSSDSDSSSSEEEEEKTSKSAVKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKGSPRPQAPKANGTSALTAQNGKAAKNSEEEEEEKKKAAVVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFDSE

Target ORF information:

RefSeq Version	NM_001284388.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284388.1

ORF Insert Sequence:

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAAGTCT 
GCCAAGGTCC CAGAGCGAAA GTTACAGGCA AATGGACCAG TGGCTAAGAA AGCTAAGAAG 
AAGGCCTCAT CCAGTGACAG TGAGGACAGC AGCGAGGAGG AGGAGGAAGT TCAAGGGCCT 
CCAGCAAAGA AGGCTGCTGT ACCTGCCAAG CGAGTCGGTC TGCCTCCTGG GAAGGCTGCA 
GCCAAAGCAT CAGAGAGTAG CAGCAGTGAA GAGTCCAGTG ATGATGATGA TGAGGAGGAC 
CAAAAGAAAC AGCCTGTCCA GAAGGGAGTT AAGCCCCAAG CCAAGGCAGC CAAAGCTCCT 
CCTAAGAAGG CCAAGAGCTC TGATTCTGAT TCTGACTCAA GCTCCGAGGA TGAGCCACCA 
AAGAACCAGA AGCCAAAGAT AACACCTGTG ACAGTTAAAG CTCAGACTAA AGCCCCTCCC 
AAACCAGCTC GAGCAGCACC TAAAATAGCC AATGGTAAAG CAGCCAGTAG CAGCAGTAGC 
AGCAGCAGCA GCAGTAGCAG TGATGACTCA GAGGAGGAGA AGGCAGCAGC CACCCCCAAG 
AAGGTCTGGA CCATAACTTC TGTCAGGGCA GAGACTGTAC CTAAAAAGCA AGTTGTGGCC 
AAGGCCCCAG TGAAAGCAGC TACCACCCCT ACCCGGAAGA GTTCTAGCAG TGAGGATTCC 
TCCAGTGACG AGGAAGAGGA GCAAAAAAAA CCCATGAAAA ATAAACCAGG TCCCTACAGT 
TCAGTCCCCC CGCCTTCTGC TCCCCCACCA AAGAAGTCTC TGGGAACCCA GCCTCCCAAG 
AAGGCTGTGG AGAAGCAGCA GCCTGTGGAA AGCAGTGAAG ACAGCAGTGA TGAGTCTGAT 
TCAAGTTCTG AAGAAGAGAA GAAACCCCCA ACTAAGGCAG TAGTCTCTAA AGCAACCACT 
AAACCACCTC CAGCAAAGAA AGCAGCAGAG AGCTCTTCAG ACAGCTCAGA CTCTGACAGC 
TCTGAGGATG ATGAAGCTCC TTCTAAGCCA GCTGGTACCA CCAAGAATTC TTCAAATAAG 
CCAGCTGTCA CCACCAAGTC ACCTGCAGTG AAGCCAGCTG CAGCCCCCAA GCAACCTGTG 
GGCGGTGGCC AGAAGCTTCT GACGAGAAAG GCTGACAGCA GCTCCAGTGA GGAAGAGAGC 
AGCTCCAGTG AGGAGGAGAA GACAAAGAAG ATGGTGGCCA CCACTAAGCC CAAGGCGACT 
GCCAAAGCAG CTCTATCTCT GCCTGCCAAG CAGGCTCCTC AGGGTAGTAG GGACAGCAGC 
TCTGATTCAG ACAGCTCCAG CAGTGAGGAG GAGGAAGAGA AGACATCTAA GTCTGCAGTT 
AAGAAGAAGC CACAGAAGGT AGCAGGAGGT GCAGCCCCTT CCAAGCCAGC CTCTGCAAAG 
AAAGGAAAGG CTGAGAGCAG CAACAGTTCT TCTTCTGATG ACTCCAGTGA GGAAGAGGAA 
GAGAAGCTCA AGGGCAAGGG CTCTCCAAGA CCACAAGCCC CCAAGGCCAA TGGCACCTCT 
GCACTGACTG CCCAGAATGG AAAAGCAGCT AAGAACAGTG AGGAGGAGGA AGAAGAAAAG 
AAAAAGGCGG CAGTGGTAGT TTCCAAATCA GGTTCATTAA AGAAGCGGAA GCAGAATGAG 
GCTGCCAAGG AGGCAGAGAC TCCTCAGGCC AAGAAGATAA AGCTTCAGAC CCCTAACACA 
TTTCCAAAAA GGAAGAAAGG AGAAAAAAGG GCATCATCCC CATTCCGAAG GGTCAGGGAG 
GAGGAAATTG AGGTGGATTC ACGAGTTGCG GACAACTCCT TTGATGCCAA GCGAGGTGCA 
GCCGGAGACT GGGGAGAGCG AGCCAATCAG GTTTTGAAGT TCACCAAAGG CAAGTCCTTT 
CGGCATGAGA AAACCAAGAA GAAGCGGGGC AGCTACCGGG GAGGCTCAAT CTCTGTCCAG 
GTCAATTCTA TTAAGTTTGA CAGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOLC1 ( NM_004741.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004732.2 Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 2, mRNA.

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CloneID	OHu15808
Clone ID Related Accession (Same CDS sequence)	NM_004741.4 , NM_004741.5
Accession Version	NM_004741.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	nucleolar and coiled-body phosphoprotein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA588285.1, AK314363.1, BC006769.2, AL520884.3 and CF145090.1. On Oct 1, 2013 this sequence version replaced NM_004741.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.91248.1, SRR1803613.9883.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAAGTCT 
GCCAAGGTCC CAGAGCGAAA GTTACAGGCA AATGGACCAG TGGCTAAGAA AGCTAAGAAG 
AAGGCCTCAT CCAGTGACAG TGAGGACAGC AGCGAGGAGG AGGAGGAAGT TCAAGGGCCT 
CCAGCAAAGA AGGCTGCTGT ACCTGCCAAG CGAGTCGGTC TGCCTCCTGG GAAGGCTGCA 
GCCAAAGCAT CAGAGAGTAG CAGCAGTGAA GAGTCCAGTG ATGATGATGA TGAGGAGGAC 
CAAAAGAAAC AGCCTGTCCA GAAGGGAGTT AAGCCCCAAG CCAAGGCAGC CAAAGCTCCT 
CCTAAGAAGG CCAAGAGCTC TGATTCTGAT TCTGACTCAA GCTCCGAGGA TGAGCCACCA 
AAGAACCAGA AGCCAAAGAT AACACCTGTG ACAGTTAAAG CTCAGACTAA AGCCCCTCCC 
AAACCAGCTC GAGCAGCACC TAAAATAGCC AATGGTAAAG CAGCCAGTAG CAGCAGTAGC 
AGCAGCAGCA GCAGTAGCAG TGATGACTCA GAGGAGGAGA AGGCAGCAGC CACCCCCAAG 
AAGACTGTAC CTAAAAAGCA AGTTGTGGCC AAGGCCCCAG TGAAAGCAGC TACCACCCCT 
ACCCGGAAGA GTTCTAGCAG TGAGGATTCC TCCAGTGACG AGGAAGAGGA GCAAAAAAAA 
CCCATGAAAA ATAAACCAGG TCCCTACAGT TCAGTCCCCC CGCCTTCTGC TCCCCCACCA 
AAGAAGTCTC TGGGAACCCA GCCTCCCAAG AAGGCTGTGG AGAAGCAGCA GCCTGTGGAA 
AGCAGTGAAG ACAGCAGTGA TGAGTCTGAT TCAAGTTCTG AAGAAGAGAA GAAACCCCCA 
ACTAAGGCAG TAGTCTCTAA AGCAACCACT AAACCACCTC CAGCAAAGAA AGCAGCAGAG 
AGCTCTTCAG ACAGCTCAGA CTCTGACAGC TCTGAGGATG ATGAAGCTCC TTCTAAGCCA 
GCTGGTACCA CCAAGAATTC TTCAAATAAG CCAGCTGTCA CCACCAAGTC ACCTGCAGTG 
AAGCCAGCTG CAGCCCCCAA GCAACCTGTG GGCGGTGGCC AGAAGCTTCT GACGAGAAAG 
GCTGACAGCA GCTCCAGTGA GGAAGAGAGC AGCTCCAGTG AGGAGGAGAA GACAAAGAAG 
ATGGTGGCCA CCACTAAGCC CAAGGCGACT GCCAAAGCAG CTCTATCTCT GCCTGCCAAG 
CAGGCTCCTC AGGGTAGTAG GGACAGCAGC TCTGATTCAG ACAGCTCCAG CAGTGAGGAG 
GAGGAAGAGA AGACATCTAA GTCTGCAGTT AAGAAGAAGC CACAGAAGGT AGCAGGAGGT 
GCAGCCCCTT CCAAGCCAGC CTCTGCAAAG AAAGGAAAGG CTGAGAGCAG CAACAGTTCT 
TCTTCTGATG ACTCCAGTGA GGAAGAGGAA GAGAAGCTCA AGGGCAAGGG CTCTCCAAGA 
CCACAAGCCC CCAAGGCCAA TGGCACCTCT GCACTGACTG CCCAGAATGG AAAAGCAGCT 
AAGAACAGTG AGGAGGAGGA AGAAGAAAAG AAAAAGGCGG CAGTGGTAGT TTCCAAATCA 
GGTTCATTAA AGAAGCGGAA GCAGAATGAG GCTGCCAAGG AGGCAGAGAC TCCTCAGGCC 
AAGAAGATAA AGCTTCAGAC CCCTAACACA TTTCCAAAAA GGAAGAAAGG AGAAAAAAGG 
GCATCATCCC CATTCCGAAG GGTCAGGGAG GAGGAAATTG AGGTGGATTC ACGAGTTGCG 
GACAACTCCT TTGATGCCAA GCGAGGTGCA GCCGGAGACT GGGGAGAGCG AGCCAATCAG 
GTTTTGAAGT TCACCAAAGG CAAGTCCTTT CGGCATGAGA AAACCAAGAA GAAGCGGGGC 
AGCTACCGGG GAGGCTCAAT CTCTGTCCAG GTCAATTCTA TTAAGTTTGA CAGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004732.2
CDS	236..2335
Translation	MADAGIRRVVPSDLYPLVLGFLRDNQLSEVANKFAKATGATQQDANASSLLDIYSFWLKSAKVPERKLQANGPVAKKAKKKASSSDSEDSSEEEEEVQGPPAKKAAVPAKRVGLPPGKAAAKASESSSSEESSDDDDEEDQKKQPVQKGVKPQAKAAKAPPKKAKSSDSDSDSSSEDEPPKNQKPKITPVTVKAQTKAPPKPARAAPKIANGKAASSSSSSSSSSSSDDSEEEKAAATPKKTVPKKQVVAKAPVKAATTPTRKSSSSEDSSSDEEEEQKKPMKNKPGPYSSVPPPSAPPPKKSLGTQPPKKAVEKQQPVESSEDSSDESDSSSEEEKKPPTKAVVSKATTKPPPAKKAAESSSDSSDSDSSEDDEAPSKPAGTTKNSSNKPAVTTKSPAVKPAAAPKQPVGGGQKLLTRKADSSSSEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSRDSSSDSDSSSSEEEEEKTSKSAVKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKGSPRPQAPKANGTSALTAQNGKAAKNSEEEEEEKKKAAVVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFDSE

Target ORF information:

RefSeq Version	NM_004741.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004741.4

ORF Insert Sequence:

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAAGTCT 
GCCAAGGTCC CAGAGCGAAA GTTACAGGCA AATGGACCAG TGGCTAAGAA AGCTAAGAAG 
AAGGCCTCAT CCAGTGACAG TGAGGACAGC AGCGAGGAGG AGGAGGAAGT TCAAGGGCCT 
CCAGCAAAGA AGGCTGCTGT ACCTGCCAAG CGAGTCGGTC TGCCTCCTGG GAAGGCTGCA 
GCCAAAGCAT CAGAGAGTAG CAGCAGTGAA GAGTCCAGTG ATGATGATGA TGAGGAGGAC 
CAAAAGAAAC AGCCTGTCCA GAAGGGAGTT AAGCCCCAAG CCAAGGCAGC CAAAGCTCCT 
CCTAAGAAGG CCAAGAGCTC TGATTCTGAT TCTGACTCAA GCTCCGAGGA TGAGCCACCA 
AAGAACCAGA AGCCAAAGAT AACACCTGTG ACAGTTAAAG CTCAGACTAA AGCCCCTCCC 
AAACCAGCTC GAGCAGCACC TAAAATAGCC AATGGTAAAG CAGCCAGTAG CAGCAGTAGC 
AGCAGCAGCA GCAGTAGCAG TGATGACTCA GAGGAGGAGA AGGCAGCAGC CACCCCCAAG 
AAGACTGTAC CTAAAAAGCA AGTTGTGGCC AAGGCCCCAG TGAAAGCAGC TACCACCCCT 
ACCCGGAAGA GTTCTAGCAG TGAGGATTCC TCCAGTGACG AGGAAGAGGA GCAAAAAAAA 
CCCATGAAAA ATAAACCAGG TCCCTACAGT TCAGTCCCCC CGCCTTCTGC TCCCCCACCA 
AAGAAGTCTC TGGGAACCCA GCCTCCCAAG AAGGCTGTGG AGAAGCAGCA GCCTGTGGAA 
AGCAGTGAAG ACAGCAGTGA TGAGTCTGAT TCAAGTTCTG AAGAAGAGAA GAAACCCCCA 
ACTAAGGCAG TAGTCTCTAA AGCAACCACT AAACCACCTC CAGCAAAGAA AGCAGCAGAG 
AGCTCTTCAG ACAGCTCAGA CTCTGACAGC TCTGAGGATG ATGAAGCTCC TTCTAAGCCA 
GCTGGTACCA CCAAGAATTC TTCAAATAAG CCAGCTGTCA CCACCAAGTC ACCTGCAGTG 
AAGCCAGCTG CAGCCCCCAA GCAACCTGTG GGCGGTGGCC AGAAGCTTCT GACGAGAAAG 
GCTGACAGCA GCTCCAGTGA GGAAGAGAGC AGCTCCAGTG AGGAGGAGAA GACAAAGAAG 
ATGGTGGCCA CCACTAAGCC CAAGGCGACT GCCAAAGCAG CTCTATCTCT GCCTGCCAAG 
CAGGCTCCTC AGGGTAGTAG GGACAGCAGC TCTGATTCAG ACAGCTCCAG CAGTGAGGAG 
GAGGAAGAGA AGACATCTAA GTCTGCAGTT AAGAAGAAGC CACAGAAGGT AGCAGGAGGT 
GCAGCCCCTT CCAAGCCAGC CTCTGCAAAG AAAGGAAAGG CTGAGAGCAG CAACAGTTCT 
TCTTCTGATG ACTCCAGTGA GGAAGAGGAA GAGAAGCTCA AGGGCAAGGG CTCTCCAAGA 
CCACAAGCCC CCAAGGCCAA TGGCACCTCT GCACTGACTG CCCAGAATGG AAAAGCAGCT 
AAGAACAGTG AGGAGGAGGA AGAAGAAAAG AAAAAGGCGG CAGTGGTAGT TTCCAAATCA 
GGTTCATTAA AGAAGCGGAA GCAGAATGAG GCTGCCAAGG AGGCAGAGAC TCCTCAGGCC 
AAGAAGATAA AGCTTCAGAC CCCTAACACA TTTCCAAAAA GGAAGAAAGG AGAAAAAAGG 
GCATCATCCC CATTCCGAAG GGTCAGGGAG GAGGAAATTG AGGTGGATTC ACGAGTTGCG 
GACAACTCCT TTGATGCCAA GCGAGGTGCA GCCGGAGACT GGGGAGAGCG AGCCAATCAG 
GTTTTGAAGT TCACCAAAGG CAAGTCCTTT CGGCATGAGA AAACCAAGAA GAAGCGGGGC 
AGCTACCGGG GAGGCTCAAT CTCTGTCCAG GTCAATTCTA TTAAGTTTGA CAGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOLC1 ( NM_004741.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004732.2 Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15808
Clone ID Related Accession (Same CDS sequence)	NM_004741.4 , NM_004741.5
Accession Version	NM_004741.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	nucleolar and coiled-body phosphoprotein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK314363.1, BC006769.2 and AL520884.3. On Jun 2, 2019 this sequence version replaced NM_004741.4. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.170269.1, SRR1803617.144290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END##

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAAGTCT 
GCCAAGGTCC CAGAGCGAAA GTTACAGGCA AATGGACCAG TGGCTAAGAA AGCTAAGAAG 
AAGGCCTCAT CCAGTGACAG TGAGGACAGC AGCGAGGAGG AGGAGGAAGT TCAAGGGCCT 
CCAGCAAAGA AGGCTGCTGT ACCTGCCAAG CGAGTCGGTC TGCCTCCTGG GAAGGCTGCA 
GCCAAAGCAT CAGAGAGTAG CAGCAGTGAA GAGTCCAGTG ATGATGATGA TGAGGAGGAC 
CAAAAGAAAC AGCCTGTCCA GAAGGGAGTT AAGCCCCAAG CCAAGGCAGC CAAAGCTCCT 
CCTAAGAAGG CCAAGAGCTC TGATTCTGAT TCTGACTCAA GCTCCGAGGA TGAGCCACCA 
AAGAACCAGA AGCCAAAGAT AACACCTGTG ACAGTTAAAG CTCAGACTAA AGCCCCTCCC 
AAACCAGCTC GAGCAGCACC TAAAATAGCC AATGGTAAAG CAGCCAGTAG CAGCAGTAGC 
AGCAGCAGCA GCAGTAGCAG TGATGACTCA GAGGAGGAGA AGGCAGCAGC CACCCCCAAG 
AAGACTGTAC CTAAAAAGCA AGTTGTGGCC AAGGCCCCAG TGAAAGCAGC TACCACCCCT 
ACCCGGAAGA GTTCTAGCAG TGAGGATTCC TCCAGTGACG AGGAAGAGGA GCAAAAAAAA 
CCCATGAAAA ATAAACCAGG TCCCTACAGT TCAGTCCCCC CGCCTTCTGC TCCCCCACCA 
AAGAAGTCTC TGGGAACCCA GCCTCCCAAG AAGGCTGTGG AGAAGCAGCA GCCTGTGGAA 
AGCAGTGAAG ACAGCAGTGA TGAGTCTGAT TCAAGTTCTG AAGAAGAGAA GAAACCCCCA 
ACTAAGGCAG TAGTCTCTAA AGCAACCACT AAACCACCTC CAGCAAAGAA AGCAGCAGAG 
AGCTCTTCAG ACAGCTCAGA CTCTGACAGC TCTGAGGATG ATGAAGCTCC TTCTAAGCCA 
GCTGGTACCA CCAAGAATTC TTCAAATAAG CCAGCTGTCA CCACCAAGTC ACCTGCAGTG 
AAGCCAGCTG CAGCCCCCAA GCAACCTGTG GGCGGTGGCC AGAAGCTTCT GACGAGAAAG 
GCTGACAGCA GCTCCAGTGA GGAAGAGAGC AGCTCCAGTG AGGAGGAGAA GACAAAGAAG 
ATGGTGGCCA CCACTAAGCC CAAGGCGACT GCCAAAGCAG CTCTATCTCT GCCTGCCAAG 
CAGGCTCCTC AGGGTAGTAG GGACAGCAGC TCTGATTCAG ACAGCTCCAG CAGTGAGGAG 
GAGGAAGAGA AGACATCTAA GTCTGCAGTT AAGAAGAAGC CACAGAAGGT AGCAGGAGGT 
GCAGCCCCTT CCAAGCCAGC CTCTGCAAAG AAAGGAAAGG CTGAGAGCAG CAACAGTTCT 
TCTTCTGATG ACTCCAGTGA GGAAGAGGAA GAGAAGCTCA AGGGCAAGGG CTCTCCAAGA 
CCACAAGCCC CCAAGGCCAA TGGCACCTCT GCACTGACTG CCCAGAATGG AAAAGCAGCT 
AAGAACAGTG AGGAGGAGGA AGAAGAAAAG AAAAAGGCGG CAGTGGTAGT TTCCAAATCA 
GGTTCATTAA AGAAGCGGAA GCAGAATGAG GCTGCCAAGG AGGCAGAGAC TCCTCAGGCC 
AAGAAGATAA AGCTTCAGAC CCCTAACACA TTTCCAAAAA GGAAGAAAGG AGAAAAAAGG 
GCATCATCCC CATTCCGAAG GGTCAGGGAG GAGGAAATTG AGGTGGATTC ACGAGTTGCG 
GACAACTCCT TTGATGCCAA GCGAGGTGCA GCCGGAGACT GGGGAGAGCG AGCCAATCAG 
GTTTTGAAGT TCACCAAAGG CAAGTCCTTT CGGCATGAGA AAACCAAGAA GAAGCGGGGC 
AGCTACCGGG GAGGCTCAAT CTCTGTCCAG GTCAATTCTA TTAAGTTTGA CAGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004732.2
CDS	23..2122
Translation	MADAGIRRVVPSDLYPLVLGFLRDNQLSEVANKFAKATGATQQDANASSLLDIYSFWLKSAKVPERKLQANGPVAKKAKKKASSSDSEDSSEEEEEVQGPPAKKAAVPAKRVGLPPGKAAAKASESSSSEESSDDDDEEDQKKQPVQKGVKPQAKAAKAPPKKAKSSDSDSDSSSEDEPPKNQKPKITPVTVKAQTKAPPKPARAAPKIANGKAASSSSSSSSSSSSDDSEEEKAAATPKKTVPKKQVVAKAPVKAATTPTRKSSSSEDSSSDEEEEQKKPMKNKPGPYSSVPPPSAPPPKKSLGTQPPKKAVEKQQPVESSEDSSDESDSSSEEEKKPPTKAVVSKATTKPPPAKKAAESSSDSSDSDSSEDDEAPSKPAGTTKNSSNKPAVTTKSPAVKPAAAPKQPVGGGQKLLTRKADSSSSEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSRDSSSDSDSSSSEEEEEKTSKSAVKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKGSPRPQAPKANGTSALTAQNGKAAKNSEEEEEEKKKAAVVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFDSE

Target ORF information:

RefSeq Version	NM_004741.5
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004741.5

ORF Insert Sequence:

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAAGTCT 
GCCAAGGTCC CAGAGCGAAA GTTACAGGCA AATGGACCAG TGGCTAAGAA AGCTAAGAAG 
AAGGCCTCAT CCAGTGACAG TGAGGACAGC AGCGAGGAGG AGGAGGAAGT TCAAGGGCCT 
CCAGCAAAGA AGGCTGCTGT ACCTGCCAAG CGAGTCGGTC TGCCTCCTGG GAAGGCTGCA 
GCCAAAGCAT CAGAGAGTAG CAGCAGTGAA GAGTCCAGTG ATGATGATGA TGAGGAGGAC 
CAAAAGAAAC AGCCTGTCCA GAAGGGAGTT AAGCCCCAAG CCAAGGCAGC CAAAGCTCCT 
CCTAAGAAGG CCAAGAGCTC TGATTCTGAT TCTGACTCAA GCTCCGAGGA TGAGCCACCA 
AAGAACCAGA AGCCAAAGAT AACACCTGTG ACAGTTAAAG CTCAGACTAA AGCCCCTCCC 
AAACCAGCTC GAGCAGCACC TAAAATAGCC AATGGTAAAG CAGCCAGTAG CAGCAGTAGC 
AGCAGCAGCA GCAGTAGCAG TGATGACTCA GAGGAGGAGA AGGCAGCAGC CACCCCCAAG 
AAGACTGTAC CTAAAAAGCA AGTTGTGGCC AAGGCCCCAG TGAAAGCAGC TACCACCCCT 
ACCCGGAAGA GTTCTAGCAG TGAGGATTCC TCCAGTGACG AGGAAGAGGA GCAAAAAAAA 
CCCATGAAAA ATAAACCAGG TCCCTACAGT TCAGTCCCCC CGCCTTCTGC TCCCCCACCA 
AAGAAGTCTC TGGGAACCCA GCCTCCCAAG AAGGCTGTGG AGAAGCAGCA GCCTGTGGAA 
AGCAGTGAAG ACAGCAGTGA TGAGTCTGAT TCAAGTTCTG AAGAAGAGAA GAAACCCCCA 
ACTAAGGCAG TAGTCTCTAA AGCAACCACT AAACCACCTC CAGCAAAGAA AGCAGCAGAG 
AGCTCTTCAG ACAGCTCAGA CTCTGACAGC TCTGAGGATG ATGAAGCTCC TTCTAAGCCA 
GCTGGTACCA CCAAGAATTC TTCAAATAAG CCAGCTGTCA CCACCAAGTC ACCTGCAGTG 
AAGCCAGCTG CAGCCCCCAA GCAACCTGTG GGCGGTGGCC AGAAGCTTCT GACGAGAAAG 
GCTGACAGCA GCTCCAGTGA GGAAGAGAGC AGCTCCAGTG AGGAGGAGAA GACAAAGAAG 
ATGGTGGCCA CCACTAAGCC CAAGGCGACT GCCAAAGCAG CTCTATCTCT GCCTGCCAAG 
CAGGCTCCTC AGGGTAGTAG GGACAGCAGC TCTGATTCAG ACAGCTCCAG CAGTGAGGAG 
GAGGAAGAGA AGACATCTAA GTCTGCAGTT AAGAAGAAGC CACAGAAGGT AGCAGGAGGT 
GCAGCCCCTT CCAAGCCAGC CTCTGCAAAG AAAGGAAAGG CTGAGAGCAG CAACAGTTCT 
TCTTCTGATG ACTCCAGTGA GGAAGAGGAA GAGAAGCTCA AGGGCAAGGG CTCTCCAAGA 
CCACAAGCCC CCAAGGCCAA TGGCACCTCT GCACTGACTG CCCAGAATGG AAAAGCAGCT 
AAGAACAGTG AGGAGGAGGA AGAAGAAAAG AAAAAGGCGG CAGTGGTAGT TTCCAAATCA 
GGTTCATTAA AGAAGCGGAA GCAGAATGAG GCTGCCAAGG AGGCAGAGAC TCCTCAGGCC 
AAGAAGATAA AGCTTCAGAC CCCTAACACA TTTCCAAAAA GGAAGAAAGG AGAAAAAAGG 
GCATCATCCC CATTCCGAAG GGTCAGGGAG GAGGAAATTG AGGTGGATTC ACGAGTTGCG 
GACAACTCCT TTGATGCCAA GCGAGGTGCA GCCGGAGACT GGGGAGAGCG AGCCAATCAG 
GTTTTGAAGT TCACCAAAGG CAAGTCCTTT CGGCATGAGA AAACCAAGAA GAAGCGGGGC 
AGCTACCGGG GAGGCTCAAT CTCTGTCCAG GTCAATTCTA TTAAGTTTGA CAGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOLC1 ( XM_005270273.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270330.1 Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant X1, mRNA.

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CloneID	OHu44669
Clone ID Related Accession (Same CDS sequence)	XM_005270273.1 , XM_005270273.2
Accession Version	XM_005270273.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nucleolar and coiled-body phosphoprotein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAACAGG 
TCTGCCAAGG TCCCAGAGCG AAAGTTACAG GCAAATGGAC CAGTGGCTAA GAAAGCTAAG 
AAGAAGGCCT CATCCAGTGA CAGTGAGGAC AGCAGCGAGG AGGAGGAGGA AGTTCAAGGG 
CCTCCAGCAA AGAAGGCTGC TGTACCTGCC AAGCGAGTCG GTCTGCCTCC TGGGAAGGCT 
GCAGCCAAAG CATCAGAGAG TAGCAGCAGT GAAGAGTCCA GTGATGATGA TGATGAGGAG 
GACCAAAAGA AACAGCCTGT CCAGAAGGGA GTTAAGCCCC AAGCCAAGGC AGCCAAAGCT 
CCTCCTAAGA AGGCCAAGAG CTCTGATTCT GATTCTGACT CAAGCTCCGA GGATGAGCCA 
CCAAAGAACC AGAAGCCAAA GATAACACCT GTGACAGTTA AAGCTCAGAC TAAAGCCCCT 
CCCAAACCAG CTCGAGCAGC ACCTAAAATA GCCAATGGTA AAGCAGCCAG TAGCAGCAGT 
AGCAGCAGCA GCAGCAGTAG CAGTGATGAC TCAGAGGAGG AGAAGGCAGC AGCCACCCCC 
AAGAAGGTCT GGACCATAAC TTCTGTCAGG GCAGAGACTG TACCTAAAAA GCAAGTTGTG 
GCCAAGGCCC CAGTGAAAGC AGCTACCACC CCTACCCGGA AGAGTTCTAG CAGTGAGGAT 
TCCTCCAGTG ACGAGGAAGA GGAGCAAAAA AAACCCATGA AAAATAAACC AGGTCCCTAC 
AGTTCAGTCC CCCCGCCTTC TGCTCCCCCA CCAAAGAAGT CTCTGGGAAC CCAGCCTCCC 
AAGAAGGCTG TGGAGAAGCA GCAGCCTGTG GAAAGCAGTG AAGACAGCAG TGATGAGTCT 
GATTCAAGTT CTGAAGAAGA GAAGAAACCC CCAACTAAGG CAGTAGTCTC TAAAGCAACC 
ACTAAACCAC CTCCAGCAAA GAAAGCAGCA GAGAGCTCTT CAGACAGCTC AGACTCTGAC 
AGCTCTGAGG ATGATGAAGC TCCTTCTAAG CCAGCTGGTA CCACCAAGAA TTCTTCAAAT 
AAGCCAGCTG TCACCACCAA GTCACCTGCA GTGAAGCCAG CTGCAGCCCC CAAGCAACCT 
GTGGGCGGTG GCCAGAAGCT TCTGACGAGA AAGGCTGACA GCAGCTCCAG TGAGGAAGAG 
AGCAGCTCCA GTGAGGAGGA GAAGACAAAG AAGATGGTGG CCACCACTAA GCCCAAGGCG 
ACTGCCAAAG CAGCTCTATC TCTGCCTGCC AAGCAGGCTC CTCAGGGTAG TAGGGACAGC 
AGCTCTGATT CAGACAGCTC CAGCAGTGAG GAGGAGGAAG AGAAGACATC TAAGTCTGCA 
GTTAAGAAGA AGCCACAGAA GGTAGCAGGA GGTGCAGCCC CTTCCAAGCC AGCCTCTGCA 
AAGAAAGGAA AGGCTGAGAG CAGCAACAGT TCTTCTTCTG ATGACTCCAG TGAGGAAGAG 
GAAGAGAAGC TCAAGGGCAA GGGCTCTCCA AGACCACAAG CCCCCAAGGC CAATGGCACC 
TCTGCACTGA CTGCCCAGAA TGGAAAAGCA GCTAAGAACA GTGAGGAGGA GGAAGAAGAA 
AAGAAAAAGG CGGCAGTGGT AGTTTCCAAA TCAGGTTCAT TAAAGAAGCG GAAGCAGAAT 
GAGGCTGCCA AGGAGGCAGA GACTCCTCAG GCCAAGAAGA TAAAGCTTCA GACCCCTAAC 
ACATTTCCAA AAAGGAAGAA AGGAGAAAAA AGGGCATCAT CCCCATTCCG AAGGGTCAGG 
GAGGAGGAAA TTGAGGTGGA TTCACGAGTT GCGGACAACT CCTTTGATGC CAAGCGAGGT 
GCAGCCGGAG ACTGGGGAGA GCGAGCCAAT CAGGTTTTGA AGTTCACCAA AGGCAAGTCC 
TTTCGGCATG AGAAAACCAA GAAGAAGCGG GGCAGCTACC GGGGAGGCTC AATCTCTGTC 
CAGGTCAATT CTATTAAGTT TGACAGCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270330.1
CDS	210..2342
Translation	MADAGIRRVVPSDLYPLVLGFLRDNQLSEVANKFAKATGATQQDANASSLLDIYSFWLNRSAKVPERKLQANGPVAKKAKKKASSSDSEDSSEEEEEVQGPPAKKAAVPAKRVGLPPGKAAAKASESSSSEESSDDDDEEDQKKQPVQKGVKPQAKAAKAPPKKAKSSDSDSDSSSEDEPPKNQKPKITPVTVKAQTKAPPKPARAAPKIANGKAASSSSSSSSSSSSDDSEEEKAAATPKKVWTITSVRAETVPKKQVVAKAPVKAATTPTRKSSSSEDSSSDEEEEQKKPMKNKPGPYSSVPPPSAPPPKKSLGTQPPKKAVEKQQPVESSEDSSDESDSSSEEEKKPPTKAVVSKATTKPPPAKKAAESSSDSSDSDSSEDDEAPSKPAGTTKNSSNKPAVTTKSPAVKPAAAPKQPVGGGQKLLTRKADSSSSEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSRDSSSDSDSSSSEEEEEKTSKSAVKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKGSPRPQAPKANGTSALTAQNGKAAKNSEEEEEEKKKAAVVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFDSE

Target ORF information:

RefSeq Version	XM_005270273.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270273.1

ORF Insert Sequence:

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAACAGG 
TCTGCCAAGG TCCCAGAGCG AAAGTTACAG GCAAATGGAC CAGTGGCTAA GAAAGCTAAG 
AAGAAGGCCT CATCCAGTGA CAGTGAGGAC AGCAGCGAGG AGGAGGAGGA AGTTCAAGGG 
CCTCCAGCAA AGAAGGCTGC TGTACCTGCC AAGCGAGTCG GTCTGCCTCC TGGGAAGGCT 
GCAGCCAAAG CATCAGAGAG TAGCAGCAGT GAAGAGTCCA GTGATGATGA TGATGAGGAG 
GACCAAAAGA AACAGCCTGT CCAGAAGGGA GTTAAGCCCC AAGCCAAGGC AGCCAAAGCT 
CCTCCTAAGA AGGCCAAGAG CTCTGATTCT GATTCTGACT CAAGCTCCGA GGATGAGCCA 
CCAAAGAACC AGAAGCCAAA GATAACACCT GTGACAGTTA AAGCTCAGAC TAAAGCCCCT 
CCCAAACCAG CTCGAGCAGC ACCTAAAATA GCCAATGGTA AAGCAGCCAG TAGCAGCAGT 
AGCAGCAGCA GCAGCAGTAG CAGTGATGAC TCAGAGGAGG AGAAGGCAGC AGCCACCCCC 
AAGAAGGTCT GGACCATAAC TTCTGTCAGG GCAGAGACTG TACCTAAAAA GCAAGTTGTG 
GCCAAGGCCC CAGTGAAAGC AGCTACCACC CCTACCCGGA AGAGTTCTAG CAGTGAGGAT 
TCCTCCAGTG ACGAGGAAGA GGAGCAAAAA AAACCCATGA AAAATAAACC AGGTCCCTAC 
AGTTCAGTCC CCCCGCCTTC TGCTCCCCCA CCAAAGAAGT CTCTGGGAAC CCAGCCTCCC 
AAGAAGGCTG TGGAGAAGCA GCAGCCTGTG GAAAGCAGTG AAGACAGCAG TGATGAGTCT 
GATTCAAGTT CTGAAGAAGA GAAGAAACCC CCAACTAAGG CAGTAGTCTC TAAAGCAACC 
ACTAAACCAC CTCCAGCAAA GAAAGCAGCA GAGAGCTCTT CAGACAGCTC AGACTCTGAC 
AGCTCTGAGG ATGATGAAGC TCCTTCTAAG CCAGCTGGTA CCACCAAGAA TTCTTCAAAT 
AAGCCAGCTG TCACCACCAA GTCACCTGCA GTGAAGCCAG CTGCAGCCCC CAAGCAACCT 
GTGGGCGGTG GCCAGAAGCT TCTGACGAGA AAGGCTGACA GCAGCTCCAG TGAGGAAGAG 
AGCAGCTCCA GTGAGGAGGA GAAGACAAAG AAGATGGTGG CCACCACTAA GCCCAAGGCG 
ACTGCCAAAG CAGCTCTATC TCTGCCTGCC AAGCAGGCTC CTCAGGGTAG TAGGGACAGC 
AGCTCTGATT CAGACAGCTC CAGCAGTGAG GAGGAGGAAG AGAAGACATC TAAGTCTGCA 
GTTAAGAAGA AGCCACAGAA GGTAGCAGGA GGTGCAGCCC CTTCCAAGCC AGCCTCTGCA 
AAGAAAGGAA AGGCTGAGAG CAGCAACAGT TCTTCTTCTG ATGACTCCAG TGAGGAAGAG 
GAAGAGAAGC TCAAGGGCAA GGGCTCTCCA AGACCACAAG CCCCCAAGGC CAATGGCACC 
TCTGCACTGA CTGCCCAGAA TGGAAAAGCA GCTAAGAACA GTGAGGAGGA GGAAGAAGAA 
AAGAAAAAGG CGGCAGTGGT AGTTTCCAAA TCAGGTTCAT TAAAGAAGCG GAAGCAGAAT 
GAGGCTGCCA AGGAGGCAGA GACTCCTCAG GCCAAGAAGA TAAAGCTTCA GACCCCTAAC 
ACATTTCCAA AAAGGAAGAA AGGAGAAAAA AGGGCATCAT CCCCATTCCG AAGGGTCAGG 
GAGGAGGAAA TTGAGGTGGA TTCACGAGTT GCGGACAACT CCTTTGATGC CAAGCGAGGT 
GCAGCCGGAG ACTGGGGAGA GCGAGCCAAT CAGGTTTTGA AGTTCACCAA AGGCAAGTCC 
TTTCGGCATG AGAAAACCAA GAAGAAGCGG GGCAGCTACC GGGGAGGCTC AATCTCTGTC 
CAGGTCAATT CTATTAAGTT TGACAGCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOLC1 ( XM_005270273.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270330.1 Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant X1, mRNA.

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CloneID	OHu44669
Clone ID Related Accession (Same CDS sequence)	XM_005270273.1 , XM_005270273.2
Accession Version	XM_005270273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nucleolar and coiled-body phosphoprotein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005270273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAACAGG 
TCTGCCAAGG TCCCAGAGCG AAAGTTACAG GCAAATGGAC CAGTGGCTAA GAAAGCTAAG 
AAGAAGGCCT CATCCAGTGA CAGTGAGGAC AGCAGCGAGG AGGAGGAGGA AGTTCAAGGG 
CCTCCAGCAA AGAAGGCTGC TGTACCTGCC AAGCGAGTCG GTCTGCCTCC TGGGAAGGCT 
GCAGCCAAAG CATCAGAGAG TAGCAGCAGT GAAGAGTCCA GTGATGATGA TGATGAGGAG 
GACCAAAAGA AACAGCCTGT CCAGAAGGGA GTTAAGCCCC AAGCCAAGGC AGCCAAAGCT 
CCTCCTAAGA AGGCCAAGAG CTCTGATTCT GATTCTGACT CAAGCTCCGA GGATGAGCCA 
CCAAAGAACC AGAAGCCAAA GATAACACCT GTGACAGTTA AAGCTCAGAC TAAAGCCCCT 
CCCAAACCAG CTCGAGCAGC ACCTAAAATA GCCAATGGTA AAGCAGCCAG TAGCAGCAGT 
AGCAGCAGCA GCAGCAGTAG CAGTGATGAC TCAGAGGAGG AGAAGGCAGC AGCCACCCCC 
AAGAAGGTCT GGACCATAAC TTCTGTCAGG GCAGAGACTG TACCTAAAAA GCAAGTTGTG 
GCCAAGGCCC CAGTGAAAGC AGCTACCACC CCTACCCGGA AGAGTTCTAG CAGTGAGGAT 
TCCTCCAGTG ACGAGGAAGA GGAGCAAAAA AAACCCATGA AAAATAAACC AGGTCCCTAC 
AGTTCAGTCC CCCCGCCTTC TGCTCCCCCA CCAAAGAAGT CTCTGGGAAC CCAGCCTCCC 
AAGAAGGCTG TGGAGAAGCA GCAGCCTGTG GAAAGCAGTG AAGACAGCAG TGATGAGTCT 
GATTCAAGTT CTGAAGAAGA GAAGAAACCC CCAACTAAGG CAGTAGTCTC TAAAGCAACC 
ACTAAACCAC CTCCAGCAAA GAAAGCAGCA GAGAGCTCTT CAGACAGCTC AGACTCTGAC 
AGCTCTGAGG ATGATGAAGC TCCTTCTAAG CCAGCTGGTA CCACCAAGAA TTCTTCAAAT 
AAGCCAGCTG TCACCACCAA GTCACCTGCA GTGAAGCCAG CTGCAGCCCC CAAGCAACCT 
GTGGGCGGTG GCCAGAAGCT TCTGACGAGA AAGGCTGACA GCAGCTCCAG TGAGGAAGAG 
AGCAGCTCCA GTGAGGAGGA GAAGACAAAG AAGATGGTGG CCACCACTAA GCCCAAGGCG 
ACTGCCAAAG CAGCTCTATC TCTGCCTGCC AAGCAGGCTC CTCAGGGTAG TAGGGACAGC 
AGCTCTGATT CAGACAGCTC CAGCAGTGAG GAGGAGGAAG AGAAGACATC TAAGTCTGCA 
GTTAAGAAGA AGCCACAGAA GGTAGCAGGA GGTGCAGCCC CTTCCAAGCC AGCCTCTGCA 
AAGAAAGGAA AGGCTGAGAG CAGCAACAGT TCTTCTTCTG ATGACTCCAG TGAGGAAGAG 
GAAGAGAAGC TCAAGGGCAA GGGCTCTCCA AGACCACAAG CCCCCAAGGC CAATGGCACC 
TCTGCACTGA CTGCCCAGAA TGGAAAAGCA GCTAAGAACA GTGAGGAGGA GGAAGAAGAA 
AAGAAAAAGG CGGCAGTGGT AGTTTCCAAA TCAGGTTCAT TAAAGAAGCG GAAGCAGAAT 
GAGGCTGCCA AGGAGGCAGA GACTCCTCAG GCCAAGAAGA TAAAGCTTCA GACCCCTAAC 
ACATTTCCAA AAAGGAAGAA AGGAGAAAAA AGGGCATCAT CCCCATTCCG AAGGGTCAGG 
GAGGAGGAAA TTGAGGTGGA TTCACGAGTT GCGGACAACT CCTTTGATGC CAAGCGAGGT 
GCAGCCGGAG ACTGGGGAGA GCGAGCCAAT CAGGTTTTGA AGTTCACCAA AGGCAAGTCC 
TTTCGGCATG AGAAAACCAA GAAGAAGCGG GGCAGCTACC GGGGAGGCTC AATCTCTGTC 
CAGGTCAATT CTATTAAGTT TGACAGCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270330.1
CDS	42..2174
Translation	MADAGIRRVVPSDLYPLVLGFLRDNQLSEVANKFAKATGATQQDANASSLLDIYSFWLNRSAKVPERKLQANGPVAKKAKKKASSSDSEDSSEEEEEVQGPPAKKAAVPAKRVGLPPGKAAAKASESSSSEESSDDDDEEDQKKQPVQKGVKPQAKAAKAPPKKAKSSDSDSDSSSEDEPPKNQKPKITPVTVKAQTKAPPKPARAAPKIANGKAASSSSSSSSSSSSDDSEEEKAAATPKKVWTITSVRAETVPKKQVVAKAPVKAATTPTRKSSSSEDSSSDEEEEQKKPMKNKPGPYSSVPPPSAPPPKKSLGTQPPKKAVEKQQPVESSEDSSDESDSSSEEEKKPPTKAVVSKATTKPPPAKKAAESSSDSSDSDSSEDDEAPSKPAGTTKNSSNKPAVTTKSPAVKPAAAPKQPVGGGQKLLTRKADSSSSEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSRDSSSDSDSSSSEEEEEKTSKSAVKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKGSPRPQAPKANGTSALTAQNGKAAKNSEEEEEEKKKAAVVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFDSE

Target ORF information:

RefSeq Version	XM_005270273.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270273.2

ORF Insert Sequence:

ATGGCGGACG CCGGCATTCG CCGCGTGGTT CCCAGCGACC TGTATCCCCT CGTGCTCGGC 
TTCCTGCGCG ATAACCAACT CTCAGAGGTG GCCAATAAGT TCGCCAAAGC GACAGGAGCT 
ACACAGCAGG ATGCCAATGC CTCTTCCCTC TTAGACATCT ATAGCTTCTG GCTCAACAGG 
TCTGCCAAGG TCCCAGAGCG AAAGTTACAG GCAAATGGAC CAGTGGCTAA GAAAGCTAAG 
AAGAAGGCCT CATCCAGTGA CAGTGAGGAC AGCAGCGAGG AGGAGGAGGA AGTTCAAGGG 
CCTCCAGCAA AGAAGGCTGC TGTACCTGCC AAGCGAGTCG GTCTGCCTCC TGGGAAGGCT 
GCAGCCAAAG CATCAGAGAG TAGCAGCAGT GAAGAGTCCA GTGATGATGA TGATGAGGAG 
GACCAAAAGA AACAGCCTGT CCAGAAGGGA GTTAAGCCCC AAGCCAAGGC AGCCAAAGCT 
CCTCCTAAGA AGGCCAAGAG CTCTGATTCT GATTCTGACT CAAGCTCCGA GGATGAGCCA 
CCAAAGAACC AGAAGCCAAA GATAACACCT GTGACAGTTA AAGCTCAGAC TAAAGCCCCT 
CCCAAACCAG CTCGAGCAGC ACCTAAAATA GCCAATGGTA AAGCAGCCAG TAGCAGCAGT 
AGCAGCAGCA GCAGCAGTAG CAGTGATGAC TCAGAGGAGG AGAAGGCAGC AGCCACCCCC 
AAGAAGGTCT GGACCATAAC TTCTGTCAGG GCAGAGACTG TACCTAAAAA GCAAGTTGTG 
GCCAAGGCCC CAGTGAAAGC AGCTACCACC CCTACCCGGA AGAGTTCTAG CAGTGAGGAT 
TCCTCCAGTG ACGAGGAAGA GGAGCAAAAA AAACCCATGA AAAATAAACC AGGTCCCTAC 
AGTTCAGTCC CCCCGCCTTC TGCTCCCCCA CCAAAGAAGT CTCTGGGAAC CCAGCCTCCC 
AAGAAGGCTG TGGAGAAGCA GCAGCCTGTG GAAAGCAGTG AAGACAGCAG TGATGAGTCT 
GATTCAAGTT CTGAAGAAGA GAAGAAACCC CCAACTAAGG CAGTAGTCTC TAAAGCAACC 
ACTAAACCAC CTCCAGCAAA GAAAGCAGCA GAGAGCTCTT CAGACAGCTC AGACTCTGAC 
AGCTCTGAGG ATGATGAAGC TCCTTCTAAG CCAGCTGGTA CCACCAAGAA TTCTTCAAAT 
AAGCCAGCTG TCACCACCAA GTCACCTGCA GTGAAGCCAG CTGCAGCCCC CAAGCAACCT 
GTGGGCGGTG GCCAGAAGCT TCTGACGAGA AAGGCTGACA GCAGCTCCAG TGAGGAAGAG 
AGCAGCTCCA GTGAGGAGGA GAAGACAAAG AAGATGGTGG CCACCACTAA GCCCAAGGCG 
ACTGCCAAAG CAGCTCTATC TCTGCCTGCC AAGCAGGCTC CTCAGGGTAG TAGGGACAGC 
AGCTCTGATT CAGACAGCTC CAGCAGTGAG GAGGAGGAAG AGAAGACATC TAAGTCTGCA 
GTTAAGAAGA AGCCACAGAA GGTAGCAGGA GGTGCAGCCC CTTCCAAGCC AGCCTCTGCA 
AAGAAAGGAA AGGCTGAGAG CAGCAACAGT TCTTCTTCTG ATGACTCCAG TGAGGAAGAG 
GAAGAGAAGC TCAAGGGCAA GGGCTCTCCA AGACCACAAG CCCCCAAGGC CAATGGCACC 
TCTGCACTGA CTGCCCAGAA TGGAAAAGCA GCTAAGAACA GTGAGGAGGA GGAAGAAGAA 
AAGAAAAAGG CGGCAGTGGT AGTTTCCAAA TCAGGTTCAT TAAAGAAGCG GAAGCAGAAT 
GAGGCTGCCA AGGAGGCAGA GACTCCTCAG GCCAAGAAGA TAAAGCTTCA GACCCCTAAC 
ACATTTCCAA AAAGGAAGAA AGGAGAAAAA AGGGCATCAT CCCCATTCCG AAGGGTCAGG 
GAGGAGGAAA TTGAGGTGGA TTCACGAGTT GCGGACAACT CCTTTGATGC CAAGCGAGGT 
GCAGCCGGAG ACTGGGGAGA GCGAGCCAAT CAGGTTTTGA AGTTCACCAA AGGCAAGTCC 
TTTCGGCATG AGAAAACCAA GAAGAAGCGG GGCAGCTACC GGGGAGGCTC AATCTCTGTC 
CAGGTCAATT CTATTAAGTT TGACAGCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biophysical characterization of the structural change of Nopp140, an intrinsically disordered protein, in the interaction with CK2?.
Biochemical and biophysical research communications477(2)181-7(2016 08)
Na JH,Lee WK,Kim Y,Jeong C,Song SS,Cha SS,Han KH,Shin YK,Yu YG

Cell-fate determination by ubiquitin-dependent regulation of translation.
Nature525(7570)523-7(2015 Sep)
Werner A,Iwasaki S,McGourty CA,Medina-Ruiz S,Teerikorpi N,Fedrigo I,Ingolia NT,Rape M

Identification of NS1 domains of avian H5N1 influenza virus which influence the interaction with the NOLC1 protein.
Virus genes50(2)238-44(2015 Apr)
Zhu CY,Zheng FL,She XS,Zhao D,Gu Y,Duan YT,Chang AK,Liu HS

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NOLC1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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