CHRDL1 cDNA ORF clone, Homo sapiens(human)

The following CHRDL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRDL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu10864 NM_145234.4
Latest version!
Homo sapiens chordin like 1 (CHRDL1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu07955 NM_001143982.2
Latest version!
Homo sapiens chordin like 1 (CHRDL1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu08591 NM_001143983.3
Latest version!
Homo sapiens chordin like 1 (CHRDL1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu22814 NM_001367204.1
Latest version!
Homo sapiens chordin like 1 (CHRDL1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu22814 NM_001143981.2
Latest version!
Homo sapiens chordin like 1 (CHRDL1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu54263 NM_001367206.1
Latest version!
Homo sapiens chordin like 1 (CHRDL1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu54263 NM_001367205.1
Latest version!
Homo sapiens chordin like 1 (CHRDL1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu54264 NM_001367207.1
Latest version!
Homo sapiens chordin like 1 (CHRDL1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu07955 XM_005262222.3
Latest version!
Homo sapiens chordin like 1 (CHRDL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu22814 XM_005262221.1
Latest version!
Homo sapiens chordin-like 1 (CHRDL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu54263 XM_017029959.1
Latest version!
Homo sapiens chordin-like 1 (CHRDL1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu54263 XM_005262223.1
Latest version!
Homo sapiens chordin-like 1 (CHRDL1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu54264 XM_005262224.1
Latest version!
Homo sapiens chordin-like 1 (CHRDL1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu107153 NM_001367209.1
Latest version!
Homo sapiens chordin like 1 (CHRDL1), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu107155 NM_001367208.1
Latest version!
Homo sapiens chordin like 1 (CHRDL1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OHu08591 NM_001143983.2 Homo sapiens chordin like 1 (CHRDL1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu10864 NM_145234.3 Homo sapiens chordin like 1 (CHRDL1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu07955 NM_001143982.1 Homo sapiens chordin like 1 (CHRDL1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu22814 NM_001143981.1 Homo sapiens chordin like 1 (CHRDL1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu10864
    Clone ID Related Accession (Same CDS sequence) NM_145234.3 , NM_145234.4
    Accession Version NM_145234.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP377491.1, BC002909.2 and AL049176.3. On Jan 24, 2009 this sequence version replaced NM_145234.2. Transcript Variant: This variant (3) differs in the 5' UTR and uses alternate in-frame splice sites in the coding region compared to variant 1. The resulting protein (isoform 3) is shorter but has the identical N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002909.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG GTAAAAAAGC AAAAGAACTT CCAGGCCAAA GCTTTGACAA TAAAGGCTAC
    TTCTGCGGGG AAGAAACGAT GCCTGTGTAT GAGTCTGTAT TCATGGAGGA TGGGGAGACA
    ACCAGAAAAA TAGCACTGGA GACTGAGAGA CCACCTCAGG TAGAGGTCCA CGTTTGGACT
    ATTCGAAAGG GCATTCTCCA GCACTTCCAT ATTGAGAAGA TCTCCAAGAG GATGTTTGAG
    GAGCTTCCTC ACTTCAAGCT GGTGACCAGA ACAACCCTGA GCCAGTGGAA GATCTTCACC
    GAAGGAGAAG CTCAGATCAG CCAGATGTGT TCAAGTCGTG TATGCAGAAC AGAGCTTGAA
    GATTTAGTCA AGGTTTTGTA CCTGGAGAGA TCTGAAAAGG GCCACTGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_660277.2
    CDS198..1568
    Translation

    Target ORF information:

    RefSeq Version NM_145234.3
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_145234.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG GTAAAAAAGC AAAAGAACTT CCAGGCCAAA GCTTTGACAA TAAAGGCTAC
    TTCTGCGGGG AAGAAACGAT GCCTGTGTAT GAGTCTGTAT TCATGGAGGA TGGGGAGACA
    ACCAGAAAAA TAGCACTGGA GACTGAGAGA CCACCTCAGG TAGAGGTCCA CGTTTGGACT
    ATTCGAAAGG GCATTCTCCA GCACTTCCAT ATTGAGAAGA TCTCCAAGAG GATGTTTGAG
    GAGCTTCCTC ACTTCAAGCT GGTGACCAGA ACAACCCTGA GCCAGTGGAA GATCTTCACC
    GAAGGAGAAG CTCAGATCAG CCAGATGTGT TCAAGTCGTG TATGCAGAAC AGAGCTTGAA
    GATTTAGTCA AGGTTTTGTA CCTGGAGAGA TCTGAAAAGG GCCACTGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10864
    Clone ID Related Accession (Same CDS sequence) NM_145234.3 , NM_145234.4
    Accession Version NM_145234.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP377491.1, BC002909.2 and AL049176.3. On Jun 1, 2019 this sequence version replaced NM_145234.3. Transcript Variant: This variant (3) differs in the 5' UTR and uses alternate in-frame splice sites in the coding region compared to variant 1. The resulting protein (isoform 3) is shorter but has the identical N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002909.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG GTAAAAAAGC AAAAGAACTT CCAGGCCAAA GCTTTGACAA TAAAGGCTAC
    TTCTGCGGGG AAGAAACGAT GCCTGTGTAT GAGTCTGTAT TCATGGAGGA TGGGGAGACA
    ACCAGAAAAA TAGCACTGGA GACTGAGAGA CCACCTCAGG TAGAGGTCCA CGTTTGGACT
    ATTCGAAAGG GCATTCTCCA GCACTTCCAT ATTGAGAAGA TCTCCAAGAG GATGTTTGAG
    GAGCTTCCTC ACTTCAAGCT GGTGACCAGA ACAACCCTGA GCCAGTGGAA GATCTTCACC
    GAAGGAGAAG CTCAGATCAG CCAGATGTGT TCAAGTCGTG TATGCAGAAC AGAGCTTGAA
    GATTTAGTCA AGGTTTTGTA CCTGGAGAGA TCTGAAAAGG GCCACTGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_660277.2
    CDS167..1537
    Translation

    Target ORF information:

    RefSeq Version NM_145234.4
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_145234.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG GTAAAAAAGC AAAAGAACTT CCAGGCCAAA GCTTTGACAA TAAAGGCTAC
    TTCTGCGGGG AAGAAACGAT GCCTGTGTAT GAGTCTGTAT TCATGGAGGA TGGGGAGACA
    ACCAGAAAAA TAGCACTGGA GACTGAGAGA CCACCTCAGG TAGAGGTCCA CGTTTGGACT
    ATTCGAAAGG GCATTCTCCA GCACTTCCAT ATTGAGAAGA TCTCCAAGAG GATGTTTGAG
    GAGCTTCCTC ACTTCAAGCT GGTGACCAGA ACAACCCTGA GCCAGTGGAA GATCTTCACC
    GAAGGAGAAG CTCAGATCAG CCAGATGTGT TCAAGTCGTG TATGCAGAAC AGAGCTTGAA
    GATTTAGTCA AGGTTTTGTA CCTGGAGAGA TCTGAAAAGG GCCACTGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07955
    Clone ID Related Accession (Same CDS sequence) NM_001143982.1 , XM_005262222.3 , NM_001143982.2
    Accession Version NM_001143982.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591489.11, AK312270.1, BC002909.2 and AL049176.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the identical N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.228961.1, SRR1803614.143511.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG GTAAAAAAGC AAAAGAAGAA CTTCCAGGCC AAAGCTTTGA CAATAAAGGC
    TACTTCTGCG GGGAAGAAAC GATGCCTGTG TATGAGTCTG TATTCATGGA GGATGGGGAG
    ACAACCAGAA AAATAGCACT GGAGACTGAG AGACCACCTC AGGTAGAGGT CCACGTTTGG
    ACTATTCGAA AGGGCATTCT CCAGCACTTC CATATTGAGA AGATCTCCAA GAGGATGTTT
    GAGGAGCTTC CTCACTTCAA GCTGGTGACC AGAACAACCC TGAGCCAGTG GAAGATCTTC
    ACCGAAGGAG AAGCTCAGAT CAGCCAGATG TGTTCAAGTC GTGTATGCAG AACAGAGCTT
    GAAGATTTAG TCAAGGTTTT GTACCTGGAG AGATCTGAAA AGGGCCACTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137454.1
    CDS350..1723
    Translation

    Target ORF information:

    RefSeq Version NM_001143982.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143982.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG GTAAAAAAGC AAAAGAAGAA CTTCCAGGCC AAAGCTTTGA CAATAAAGGC
    TACTTCTGCG GGGAAGAAAC GATGCCTGTG TATGAGTCTG TATTCATGGA GGATGGGGAG
    ACAACCAGAA AAATAGCACT GGAGACTGAG AGACCACCTC AGGTAGAGGT CCACGTTTGG
    ACTATTCGAA AGGGCATTCT CCAGCACTTC CATATTGAGA AGATCTCCAA GAGGATGTTT
    GAGGAGCTTC CTCACTTCAA GCTGGTGACC AGAACAACCC TGAGCCAGTG GAAGATCTTC
    ACCGAAGGAG AAGCTCAGAT CAGCCAGATG TGTTCAAGTC GTGTATGCAG AACAGAGCTT
    GAAGATTTAG TCAAGGTTTT GTACCTGGAG AGATCTGAAA AGGGCCACTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07955
    Clone ID Related Accession (Same CDS sequence) NM_001143982.1 , XM_005262222.3 , NM_001143982.2
    Accession Version NM_001143982.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK312270.1, BC002909.2 and AL049176.3. On May 31, 2019 this sequence version replaced NM_001143982.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the identical N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.143511.1, SRR1660803.228961.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG GTAAAAAAGC AAAAGAAGAA CTTCCAGGCC AAAGCTTTGA CAATAAAGGC
    TACTTCTGCG GGGAAGAAAC GATGCCTGTG TATGAGTCTG TATTCATGGA GGATGGGGAG
    ACAACCAGAA AAATAGCACT GGAGACTGAG AGACCACCTC AGGTAGAGGT CCACGTTTGG
    ACTATTCGAA AGGGCATTCT CCAGCACTTC CATATTGAGA AGATCTCCAA GAGGATGTTT
    GAGGAGCTTC CTCACTTCAA GCTGGTGACC AGAACAACCC TGAGCCAGTG GAAGATCTTC
    ACCGAAGGAG AAGCTCAGAT CAGCCAGATG TGTTCAAGTC GTGTATGCAG AACAGAGCTT
    GAAGATTTAG TCAAGGTTTT GTACCTGGAG AGATCTGAAA AGGGCCACTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137454.1
    CDS109..1482
    Translation

    Target ORF information:

    RefSeq Version NM_001143982.2
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143982.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG GTAAAAAAGC AAAAGAAGAA CTTCCAGGCC AAAGCTTTGA CAATAAAGGC
    TACTTCTGCG GGGAAGAAAC GATGCCTGTG TATGAGTCTG TATTCATGGA GGATGGGGAG
    ACAACCAGAA AAATAGCACT GGAGACTGAG AGACCACCTC AGGTAGAGGT CCACGTTTGG
    ACTATTCGAA AGGGCATTCT CCAGCACTTC CATATTGAGA AGATCTCCAA GAGGATGTTT
    GAGGAGCTTC CTCACTTCAA GCTGGTGACC AGAACAACCC TGAGCCAGTG GAAGATCTTC
    ACCGAAGGAG AAGCTCAGAT CAGCCAGATG TGTTCAAGTC GTGTATGCAG AACAGAGCTT
    GAAGATTTAG TCAAGGTTTT GTACCTGGAG AGATCTGAAA AGGGCCACTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08591
    Clone ID Related Accession (Same CDS sequence) NM_001143983.3 , NM_001143983.2
    Accession Version NM_001143983.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK297563.1, AL049176.3 and AA057564.1. On May 31, 2019 this sequence version replaced NM_001143983.2. Transcript Variant: This variant (4) lacks two alternate exons, resulting in the loss of an in-frame segment in the central coding region, compared to variant 1. The resulting protein (isoform 4) is shorter but has the identical N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297563.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGTAGA GGTCCACGTT
    TGGACTATTC GAAAGGGCAT TCTCCAGCAC TTCCATATTG AGAAGATCTC CAAGAGGATG
    TTTGAGGAGC TTCCTCACTT CAAGCTGGTG ACCAGAACAA CCCTGAGCCA GTGGAAGATC
    TTCACCGAAG GAGAAGCTCA GATCAGCCAG ATGTGTTCAA GTCGTGTATG CAGAACAGAG
    CTTGAAGATT TAGTCAAGGT TTTGTACCTG GAGAGATCTG AAAAGGGCCA CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137455.2
    CDS109..1245
    Translation

    Target ORF information:

    RefSeq Version NM_001143983.3
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143983.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGTAGA GGTCCACGTT
    TGGACTATTC GAAAGGGCAT TCTCCAGCAC TTCCATATTG AGAAGATCTC CAAGAGGATG
    TTTGAGGAGC TTCCTCACTT CAAGCTGGTG ACCAGAACAA CCCTGAGCCA GTGGAAGATC
    TTCACCGAAG GAGAAGCTCA GATCAGCCAG ATGTGTTCAA GTCGTGTATG CAGAACAGAG
    CTTGAAGATT TAGTCAAGGT TTTGTACCTG GAGAGATCTG AAAAGGGCCA CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08591
    Clone ID Related Accession (Same CDS sequence) NM_001143983.3 , NM_001143983.2
    Accession Version NM_001143983.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC404314.1, AL591489.11, AK297563.1, AL049176.3 and AA057564.1. On Mar 16, 2010 this sequence version replaced NM_001143983.1. Transcript Variant: This variant (4) lacks two alternate exons, resulting in the loss of an in-frame segment in the central coding region, compared to variant 1. The resulting protein (isoform 4) is shorter but has the identical N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297563.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGTAGA GGTCCACGTT
    TGGACTATTC GAAAGGGCAT TCTCCAGCAC TTCCATATTG AGAAGATCTC CAAGAGGATG
    TTTGAGGAGC TTCCTCACTT CAAGCTGGTG ACCAGAACAA CCCTGAGCCA GTGGAAGATC
    TTCACCGAAG GAGAAGCTCA GATCAGCCAG ATGTGTTCAA GTCGTGTATG CAGAACAGAG
    CTTGAAGATT TAGTCAAGGT TTTGTACCTG GAGAGATCTG AAAAGGGCCA CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137455.2
    CDS350..1486
    Translation

    Target ORF information:

    RefSeq Version NM_001143983.2
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143983.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGTAGA GGTCCACGTT
    TGGACTATTC GAAAGGGCAT TCTCCAGCAC TTCCATATTG AGAAGATCTC CAAGAGGATG
    TTTGAGGAGC TTCCTCACTT CAAGCTGGTG ACCAGAACAA CCCTGAGCCA GTGGAAGATC
    TTCACCGAAG GAGAAGCTCA GATCAGCCAG ATGTGTTCAA GTCGTGTATG CAGAACAGAG
    CTTGAAGATT TAGTCAAGGT TTTGTACCTG GAGAGATCTG AAAAGGGCCA CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22814
    Clone ID Related Accession (Same CDS sequence) NM_001367204.1 , NM_001143981.1 , XM_005262221.1 , NM_001143981.2
    Accession Version NM_001367204.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-25
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591489.11 and AL049176.3. On Nov 10, 2018 this sequence version replaced XM_005262221.1. Transcript Variant: This variant (5), as well as variant 1, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.158011.1, SRR1660809.86484.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGTAGA GGTCCACGTT
    TGGACTATTC GAAAGGGCAT TCTCCAGCAC TTCCATATTG AGAAGATCTC CAAGAGGATG
    TTTGAGGAGC TTCCTCACTT CAAGCTGGTG ACCAGAACAA CCCTGAGCCA GTGGAAGATC
    TTCACCGAAG GAGAAGCTCA GATCAGCCAG ATGTGTTCAA GTCGTGTATG CAGAACAGAG
    CTTGAAGATT TAGTCAAGGT TTTGTACCTG GAGAGATCTG AAAAGGGCCA CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001354133.1
    CDS167..1543
    Translation

    Target ORF information:

    RefSeq Version NM_001367204.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001367204.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGTAGA GGTCCACGTT
    TGGACTATTC GAAAGGGCAT TCTCCAGCAC TTCCATATTG AGAAGATCTC CAAGAGGATG
    TTTGAGGAGC TTCCTCACTT CAAGCTGGTG ACCAGAACAA CCCTGAGCCA GTGGAAGATC
    TTCACCGAAG GAGAAGCTCA GATCAGCCAG ATGTGTTCAA GTCGTGTATG CAGAACAGAG
    CTTGAAGATT TAGTCAAGGT TTTGTACCTG GAGAGATCTG AAAAGGGCCA CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22814
    Clone ID Related Accession (Same CDS sequence) NM_001367204.1 , NM_001143981.1 , XM_005262221.1 , NM_001143981.2
    Accession Version NM_001143981.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-09
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591489.11, AK293106.1 and AL049176.3. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1 and 5 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.250417.1, SRR1803614.192810.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGTAGA GGTCCACGTT
    TGGACTATTC GAAAGGGCAT TCTCCAGCAC TTCCATATTG AGAAGATCTC CAAGAGGATG
    TTTGAGGAGC TTCCTCACTT CAAGCTGGTG ACCAGAACAA CCCTGAGCCA GTGGAAGATC
    TTCACCGAAG GAGAAGCTCA GATCAGCCAG ATGTGTTCAA GTCGTGTATG CAGAACAGAG
    CTTGAAGATT TAGTCAAGGT TTTGTACCTG GAGAGATCTG AAAAGGGCCA CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137453.1
    CDS350..1726
    Translation

    Target ORF information:

    RefSeq Version NM_001143981.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143981.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGTAGA GGTCCACGTT
    TGGACTATTC GAAAGGGCAT TCTCCAGCAC TTCCATATTG AGAAGATCTC CAAGAGGATG
    TTTGAGGAGC TTCCTCACTT CAAGCTGGTG ACCAGAACAA CCCTGAGCCA GTGGAAGATC
    TTCACCGAAG GAGAAGCTCA GATCAGCCAG ATGTGTTCAA GTCGTGTATG CAGAACAGAG
    CTTGAAGATT TAGTCAAGGT TTTGTACCTG GAGAGATCTG AAAAGGGCCA CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22814
    Clone ID Related Accession (Same CDS sequence) NM_001367204.1 , NM_001143981.1 , XM_005262221.1 , NM_001143981.2
    Accession Version NM_001143981.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-20
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591489.11, AK293106.1 and AL049176.3. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_001143981.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1 and 5 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.11562.1, SRR1803611.270634.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000372042.6/ ENSP00000361112.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGTAGA GGTCCACGTT
    TGGACTATTC GAAAGGGCAT TCTCCAGCAC TTCCATATTG AGAAGATCTC CAAGAGGATG
    TTTGAGGAGC TTCCTCACTT CAAGCTGGTG ACCAGAACAA CCCTGAGCCA GTGGAAGATC
    TTCACCGAAG GAGAAGCTCA GATCAGCCAG ATGTGTTCAA GTCGTGTATG CAGAACAGAG
    CTTGAAGATT TAGTCAAGGT TTTGTACCTG GAGAGATCTG AAAAGGGCCA CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137453.1
    CDS109..1485
    Translation

    Target ORF information:

    RefSeq Version NM_001143981.2
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143981.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGTAGA GGTCCACGTT
    TGGACTATTC GAAAGGGCAT TCTCCAGCAC TTCCATATTG AGAAGATCTC CAAGAGGATG
    TTTGAGGAGC TTCCTCACTT CAAGCTGGTG ACCAGAACAA CCCTGAGCCA GTGGAAGATC
    TTCACCGAAG GAGAAGCTCA GATCAGCCAG ATGTGTTCAA GTCGTGTATG CAGAACAGAG
    CTTGAAGATT TAGTCAAGGT TTTGTACCTG GAGAGATCTG AAAAGGGCCA CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu54263
    Clone ID Related Accession (Same CDS sequence) XM_005262223.1 , XM_017029959.1 , NM_001367206.1 , NM_001367205.1
    Accession Version NM_001367206.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-25
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 5 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591489.11 and AL049176.3. On Nov 10, 2018 this sequence version replaced XM_005262223.1. Transcript Variant: This variant (7), as well as variant 6, encodes isoform 5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.61170.1, SRR1803614.151192.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGAAGAACT TCCAGGCCAA AGCTTTGACA ATAAAGGCTA CTTCTGCGGG
    GAAGAAACGA TGCCTGTGTA TGAGTCTGTA TTCATGGAGG ATGGGGAGAC AACCAGAAAA
    ATAGCACTGG AGACTGAGAG ACCACCTCAG GTAGAGGTCC ACGTTTGGAC TATTCGAAAG
    GGCATTCTCC AGCACTTCCA TATTGAGAAG ATCTCCAAGA GGATGTTTGA GGAGCTTCCT
    CACTTCAAGC TGGTGACCAG AACAACCCTG AGCCAGTGGA AGATCTTCAC CGAAGGAGAA
    GCTCAGATCA GCCAGATGTG TTCAAGTCGT GTATGCAGAA CAGAGCTTGA AGATTTAGTC
    AAGGTTTTGT ACCTGGAGAG ATCTGAAAAG GGCCACTGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001354135.1
    CDS109..1470
    Translation

    Target ORF information:

    RefSeq Version NM_001367206.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001367206.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGAAGAACT TCCAGGCCAA AGCTTTGACA ATAAAGGCTA CTTCTGCGGG
    GAAGAAACGA TGCCTGTGTA TGAGTCTGTA TTCATGGAGG ATGGGGAGAC AACCAGAAAA
    ATAGCACTGG AGACTGAGAG ACCACCTCAG GTAGAGGTCC ACGTTTGGAC TATTCGAAAG
    GGCATTCTCC AGCACTTCCA TATTGAGAAG ATCTCCAAGA GGATGTTTGA GGAGCTTCCT
    CACTTCAAGC TGGTGACCAG AACAACCCTG AGCCAGTGGA AGATCTTCAC CGAAGGAGAA
    GCTCAGATCA GCCAGATGTG TTCAAGTCGT GTATGCAGAA CAGAGCTTGA AGATTTAGTC
    AAGGTTTTGT ACCTGGAGAG ATCTGAAAAG GGCCACTGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu54263
    Clone ID Related Accession (Same CDS sequence) XM_005262223.1 , XM_017029959.1 , NM_001367206.1 , NM_001367205.1
    Accession Version NM_001367205.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-25
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 5 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591489.11 and AL049176.3. On Nov 10, 2018 this sequence version replaced XM_017029959.1. Transcript Variant: This variant (6), as well as variant 7, encodes isoform 5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.236301.1, SRR1660803.35518.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGAAGAACT TCCAGGCCAA AGCTTTGACA ATAAAGGCTA CTTCTGCGGG
    GAAGAAACGA TGCCTGTGTA TGAGTCTGTA TTCATGGAGG ATGGGGAGAC AACCAGAAAA
    ATAGCACTGG AGACTGAGAG ACCACCTCAG GTAGAGGTCC ACGTTTGGAC TATTCGAAAG
    GGCATTCTCC AGCACTTCCA TATTGAGAAG ATCTCCAAGA GGATGTTTGA GGAGCTTCCT
    CACTTCAAGC TGGTGACCAG AACAACCCTG AGCCAGTGGA AGATCTTCAC CGAAGGAGAA
    GCTCAGATCA GCCAGATGTG TTCAAGTCGT GTATGCAGAA CAGAGCTTGA AGATTTAGTC
    AAGGTTTTGT ACCTGGAGAG ATCTGAAAAG GGCCACTGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001354134.1
    CDS167..1528
    Translation

    Target ORF information:

    RefSeq Version NM_001367205.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001367205.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGAAGAACT TCCAGGCCAA AGCTTTGACA ATAAAGGCTA CTTCTGCGGG
    GAAGAAACGA TGCCTGTGTA TGAGTCTGTA TTCATGGAGG ATGGGGAGAC AACCAGAAAA
    ATAGCACTGG AGACTGAGAG ACCACCTCAG GTAGAGGTCC ACGTTTGGAC TATTCGAAAG
    GGCATTCTCC AGCACTTCCA TATTGAGAAG ATCTCCAAGA GGATGTTTGA GGAGCTTCCT
    CACTTCAAGC TGGTGACCAG AACAACCCTG AGCCAGTGGA AGATCTTCAC CGAAGGAGAA
    GCTCAGATCA GCCAGATGTG TTCAAGTCGT GTATGCAGAA CAGAGCTTGA AGATTTAGTC
    AAGGTTTTGT ACCTGGAGAG ATCTGAAAAG GGCCACTGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu54264
    Clone ID Related Accession (Same CDS sequence) NM_001367207.1 , XM_005262224.1
    Accession Version NM_001367207.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-25
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 6 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591489.11 and AL049176.3. On Nov 10, 2018 this sequence version replaced XM_005262224.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.50939.1, SRR1803613.163393.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG AAGAACTTCC AGGCCAAAGC TTTGACAATA AAGGCTACTT CTGCGGGGAA
    GAAACGATGC CTGTGTATGA GTCTGTATTC ATGGAGGATG GGGAGACAAC CAGAAAAATA
    GCACTGGAGA CTGAGAGACC ACCTCAGGTA GAGGTCCACG TTTGGACTAT TCGAAAGGGC
    ATTCTCCAGC ACTTCCATAT TGAGAAGATC TCCAAGAGGA TGTTTGAGGA GCTTCCTCAC
    TTCAAGCTGG TGACCAGAAC AACCCTGAGC CAGTGGAAGA TCTTCACCGA AGGAGAAGCT
    CAGATCAGCC AGATGTGTTC AAGTCGTGTA TGCAGAACAG AGCTTGAAGA TTTAGTCAAG
    GTTTTGTACC TGGAGAGATC TGAAAAGGGC CACTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001354136.1
    CDS109..1467
    Translation

    Target ORF information:

    RefSeq Version NM_001367207.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001367207.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG AAGAACTTCC AGGCCAAAGC TTTGACAATA AAGGCTACTT CTGCGGGGAA
    GAAACGATGC CTGTGTATGA GTCTGTATTC ATGGAGGATG GGGAGACAAC CAGAAAAATA
    GCACTGGAGA CTGAGAGACC ACCTCAGGTA GAGGTCCACG TTTGGACTAT TCGAAAGGGC
    ATTCTCCAGC ACTTCCATAT TGAGAAGATC TCCAAGAGGA TGTTTGAGGA GCTTCCTCAC
    TTCAAGCTGG TGACCAGAAC AACCCTGAGC CAGTGGAAGA TCTTCACCGA AGGAGAAGCT
    CAGATCAGCC AGATGTGTTC AAGTCGTGTA TGCAGAACAG AGCTTGAAGA TTTAGTCAAG
    GTTTTGTACC TGGAGAGATC TGAAAAGGGC CACTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07955
    Clone ID Related Accession (Same CDS sequence) NM_001143982.1 , XM_005262222.3 , NM_001143982.2
    Accession Version XM_005262222.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005262222.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG GTAAAAAAGC AAAAGAAGAA CTTCCAGGCC AAAGCTTTGA CAATAAAGGC
    TACTTCTGCG GGGAAGAAAC GATGCCTGTG TATGAGTCTG TATTCATGGA GGATGGGGAG
    ACAACCAGAA AAATAGCACT GGAGACTGAG AGACCACCTC AGGTAGAGGT CCACGTTTGG
    ACTATTCGAA AGGGCATTCT CCAGCACTTC CATATTGAGA AGATCTCCAA GAGGATGTTT
    GAGGAGCTTC CTCACTTCAA GCTGGTGACC AGAACAACCC TGAGCCAGTG GAAGATCTTC
    ACCGAAGGAG AAGCTCAGAT CAGCCAGATG TGTTCAAGTC GTGTATGCAG AACAGAGCTT
    GAAGATTTAG TCAAGGTTTT GTACCTGGAG AGATCTGAAA AGGGCCACTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005262279.1
    CDS167..1540
    Translation

    Target ORF information:

    RefSeq Version XM_005262222.3
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005262222.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG GTAAAAAAGC AAAAGAAGAA CTTCCAGGCC AAAGCTTTGA CAATAAAGGC
    TACTTCTGCG GGGAAGAAAC GATGCCTGTG TATGAGTCTG TATTCATGGA GGATGGGGAG
    ACAACCAGAA AAATAGCACT GGAGACTGAG AGACCACCTC AGGTAGAGGT CCACGTTTGG
    ACTATTCGAA AGGGCATTCT CCAGCACTTC CATATTGAGA AGATCTCCAA GAGGATGTTT
    GAGGAGCTTC CTCACTTCAA GCTGGTGACC AGAACAACCC TGAGCCAGTG GAAGATCTTC
    ACCGAAGGAG AAGCTCAGAT CAGCCAGATG TGTTCAAGTC GTGTATGCAG AACAGAGCTT
    GAAGATTTAG TCAAGGTTTT GTACCTGGAG AGATCTGAAA AGGGCCACTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22814
    Clone ID Related Accession (Same CDS sequence) NM_001367204.1 , NM_001143981.1 , XM_005262221.1 , NM_001143981.2
    Accession Version XM_005262221.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGTAGA GGTCCACGTT
    TGGACTATTC GAAAGGGCAT TCTCCAGCAC TTCCATATTG AGAAGATCTC CAAGAGGATG
    TTTGAGGAGC TTCCTCACTT CAAGCTGGTG ACCAGAACAA CCCTGAGCCA GTGGAAGATC
    TTCACCGAAG GAGAAGCTCA GATCAGCCAG ATGTGTTCAA GTCGTGTATG CAGAACAGAG
    CTTGAAGATT TAGTCAAGGT TTTGTACCTG GAGAGATCTG AAAAGGGCCA CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005262278.1
    CDS167..1543
    Translation

    Target ORF information:

    RefSeq Version XM_005262221.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin-like 1 (CHRDL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005262221.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGTAGA GGTCCACGTT
    TGGACTATTC GAAAGGGCAT TCTCCAGCAC TTCCATATTG AGAAGATCTC CAAGAGGATG
    TTTGAGGAGC TTCCTCACTT CAAGCTGGTG ACCAGAACAA CCCTGAGCCA GTGGAAGATC
    TTCACCGAAG GAGAAGCTCA GATCAGCCAG ATGTGTTCAA GTCGTGTATG CAGAACAGAG
    CTTGAAGATT TAGTCAAGGT TTTGTACCTG GAGAGATCTG AAAAGGGCCA CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu54263
    Clone ID Related Accession (Same CDS sequence) XM_005262223.1 , XM_017029959.1 , NM_001367206.1 , NM_001367205.1
    Accession Version XM_005262223.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGAAGAACT TCCAGGCCAA AGCTTTGACA ATAAAGGCTA CTTCTGCGGG
    GAAGAAACGA TGCCTGTGTA TGAGTCTGTA TTCATGGAGG ATGGGGAGAC AACCAGAAAA
    ATAGCACTGG AGACTGAGAG ACCACCTCAG GTAGAGGTCC ACGTTTGGAC TATTCGAAAG
    GGCATTCTCC AGCACTTCCA TATTGAGAAG ATCTCCAAGA GGATGTTTGA GGAGCTTCCT
    CACTTCAAGC TGGTGACCAG AACAACCCTG AGCCAGTGGA AGATCTTCAC CGAAGGAGAA
    GCTCAGATCA GCCAGATGTG TTCAAGTCGT GTATGCAGAA CAGAGCTTGA AGATTTAGTC
    AAGGTTTTGT ACCTGGAGAG ATCTGAAAAG GGCCACTGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005262280.1
    CDS144..1505
    Translation

    Target ORF information:

    RefSeq Version XM_005262223.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin-like 1 (CHRDL1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005262223.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGAAGAACT TCCAGGCCAA AGCTTTGACA ATAAAGGCTA CTTCTGCGGG
    GAAGAAACGA TGCCTGTGTA TGAGTCTGTA TTCATGGAGG ATGGGGAGAC AACCAGAAAA
    ATAGCACTGG AGACTGAGAG ACCACCTCAG GTAGAGGTCC ACGTTTGGAC TATTCGAAAG
    GGCATTCTCC AGCACTTCCA TATTGAGAAG ATCTCCAAGA GGATGTTTGA GGAGCTTCCT
    CACTTCAAGC TGGTGACCAG AACAACCCTG AGCCAGTGGA AGATCTTCAC CGAAGGAGAA
    GCTCAGATCA GCCAGATGTG TTCAAGTCGT GTATGCAGAA CAGAGCTTGA AGATTTAGTC
    AAGGTTTTGT ACCTGGAGAG ATCTGAAAAG GGCCACTGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu54263
    Clone ID Related Accession (Same CDS sequence) XM_005262223.1 , XM_017029959.1 , NM_001367206.1 , NM_001367205.1
    Accession Version XM_017029959.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGAAGAACT TCCAGGCCAA AGCTTTGACA ATAAAGGCTA CTTCTGCGGG
    GAAGAAACGA TGCCTGTGTA TGAGTCTGTA TTCATGGAGG ATGGGGAGAC AACCAGAAAA
    ATAGCACTGG AGACTGAGAG ACCACCTCAG GTAGAGGTCC ACGTTTGGAC TATTCGAAAG
    GGCATTCTCC AGCACTTCCA TATTGAGAAG ATCTCCAAGA GGATGTTTGA GGAGCTTCCT
    CACTTCAAGC TGGTGACCAG AACAACCCTG AGCCAGTGGA AGATCTTCAC CGAAGGAGAA
    GCTCAGATCA GCCAGATGTG TTCAAGTCGT GTATGCAGAA CAGAGCTTGA AGATTTAGTC
    AAGGTTTTGT ACCTGGAGAG ATCTGAAAAG GGCCACTGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885448.1
    CDS167..1528
    Translation

    Target ORF information:

    RefSeq Version XM_017029959.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin-like 1 (CHRDL1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029959.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGAAGAACT TCCAGGCCAA AGCTTTGACA ATAAAGGCTA CTTCTGCGGG
    GAAGAAACGA TGCCTGTGTA TGAGTCTGTA TTCATGGAGG ATGGGGAGAC AACCAGAAAA
    ATAGCACTGG AGACTGAGAG ACCACCTCAG GTAGAGGTCC ACGTTTGGAC TATTCGAAAG
    GGCATTCTCC AGCACTTCCA TATTGAGAAG ATCTCCAAGA GGATGTTTGA GGAGCTTCCT
    CACTTCAAGC TGGTGACCAG AACAACCCTG AGCCAGTGGA AGATCTTCAC CGAAGGAGAA
    GCTCAGATCA GCCAGATGTG TTCAAGTCGT GTATGCAGAA CAGAGCTTGA AGATTTAGTC
    AAGGTTTTGT ACCTGGAGAG ATCTGAAAAG GGCCACTGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu54264
    Clone ID Related Accession (Same CDS sequence) NM_001367207.1 , XM_005262224.1
    Accession Version XM_005262224.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG AAGAACTTCC AGGCCAAAGC TTTGACAATA AAGGCTACTT CTGCGGGGAA
    GAAACGATGC CTGTGTATGA GTCTGTATTC ATGGAGGATG GGGAGACAAC CAGAAAAATA
    GCACTGGAGA CTGAGAGACC ACCTCAGGTA GAGGTCCACG TTTGGACTAT TCGAAAGGGC
    ATTCTCCAGC ACTTCCATAT TGAGAAGATC TCCAAGAGGA TGTTTGAGGA GCTTCCTCAC
    TTCAAGCTGG TGACCAGAAC AACCCTGAGC CAGTGGAAGA TCTTCACCGA AGGAGAAGCT
    CAGATCAGCC AGATGTGTTC AAGTCGTGTA TGCAGAACAG AGCTTGAAGA TTTAGTCAAG
    GTTTTGTACC TGGAGAGATC TGAAAAGGGC CACTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005262281.1
    CDS166..1524
    Translation

    Target ORF information:

    RefSeq Version XM_005262224.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin-like 1 (CHRDL1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005262224.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GACTCCTTAC CCCCAGTGAA CAATAAGGTG ACCAGCAAGT CTTGCGAGTA CAATGGGACA
    ACTTACCAAC ATGGAGAGCT GTTCGTAGCT GAAGGGCTCT TTCAGAATCG GCAACCCAAT
    CAATGCACCC AGTGCAGCTG TTCGGAGGGA AACGTGTATT GTGGTCTCAA GACTTGCCCC
    AAATTAACCT GTGCCTTCCC AGTCTCTGTT CCAGATTCCT GCTGCCGGGT ATGCAGAGGA
    GATGGAGAAC TGTCATGGGA ACATTCTGAT GGTGATATCT TCCGGCAACC TGCCAACAGA
    GAAGCAAGAC ATTCTTACCA CCGCTCTCAC TATGATCCTC CACCAAGCCG ACAGGCTGGA
    GGTCTGTCCC GCTTTCCTGG GGCCAGAAGT CACCGGGGAG CTCTTATGGA TTCCCAGCAA
    GCATCAGGAA CCATTGTGCA AATTGTCATC AATAACAAAC ACAAGCATGG ACAAGTGTGT
    GTTTCCAATG GAAAGACCTA TTCTCATGGC GAGTCCTGGC ACCCAAACCT CCGGGCATTT
    GGCATTGTGG AGTGTGTGCT ATGTACTTGT AATGTCACCA AGCAAGAGTG TAAGAAAATC
    CACTGCCCCA ATCGATACCC CTGCAAGTAT CCTCAAAAAA TAGACGGAAA ATGCTGCAAG
    GTGTGTCCAG AAGAACTTCC AGGCCAAAGC TTTGACAATA AAGGCTACTT CTGCGGGGAA
    GAAACGATGC CTGTGTATGA GTCTGTATTC ATGGAGGATG GGGAGACAAC CAGAAAAATA
    GCACTGGAGA CTGAGAGACC ACCTCAGGTA GAGGTCCACG TTTGGACTAT TCGAAAGGGC
    ATTCTCCAGC ACTTCCATAT TGAGAAGATC TCCAAGAGGA TGTTTGAGGA GCTTCCTCAC
    TTCAAGCTGG TGACCAGAAC AACCCTGAGC CAGTGGAAGA TCTTCACCGA AGGAGAAGCT
    CAGATCAGCC AGATGTGTTC AAGTCGTGTA TGCAGAACAG AGCTTGAAGA TTTAGTCAAG
    GTTTTGTACC TGGAGAGATC TGAAAAGGGC CACTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107153
    Clone ID Related Accession (Same CDS sequence) NM_001367209.1
    Accession Version NM_001367209.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 8 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591489.11 and AL049176.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.180458.1, SRR1803615.218375.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGCATT CTCCAGCACT
    TCCATATTGA GAAGATCTCC AAGAGGATGT TTGAGGAGCT TCCTCACTTC AAGCTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001354138.1
    CDS167..1366
    Translation

    Target ORF information:

    RefSeq Version NM_001367209.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001367209.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGGTAAAAA AGCAAAAGAA GAACTTCCAG GCCAAAGCTT TGACAATAAA
    GGCTACTTCT GCGGGGAAGA AACGATGCCT GTGTATGAGT CTGTATTCAT GGAGGATGGG
    GAGACAACCA GAAAAATAGC ACTGGAGACT GAGAGACCAC CTCAGGCATT CTCCAGCACT
    TCCATATTGA GAAGATCTCC AAGAGGATGT TTGAGGAGCT TCCTCACTTC AAGCTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107155
    Clone ID Related Accession (Same CDS sequence) NM_001367208.1
    Accession Version NM_001367208.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Homo sapiens(human)
    Product chordin-like protein 1 isoform 7 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591489.11 and AL049176.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.109179.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGAACTTCC AGGCCAAAGC TTTGACAATA AAGGCTACTT CTGCGGGGAA
    GAAACGATGC CTGTGTATGA GTCTGTATTC ATGGAGGATG GGGAGACAAC CAGAAAAATA
    GCACTGGAGA CTGAGAGACC ACCTCAGGTA GAGGTCCACG TTTGGACTAT TCGAAAGGGC
    ATTCTCCAGC ACTTCCATAT TGAGAAGATC TCCAAGAGGA TGTTTGAGGA GCTTCCTCAC
    TTCAAGCTGG TGACCAGAAC AACCCTGAGC CAGTGGAAGA TCTTCACCGA AGGAGAAGCT
    CAGATCAGCC AGATGTGTTC AAGTCGTGTA TGCAGAACAG AGCTTGAAGA TTTAGTCAAG
    GTTTTGTACC TGGAGAGATC TGAAAAGGGC CACTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001354137.1
    CDS109..1467
    Translation

    Target ORF information:

    RefSeq Version NM_001367208.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin like 1 (CHRDL1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001367208.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGAAAAA AGTGGAAAAT GGGAGGCATG AAATACATCT TTTCGTTGTT GTTCTTTCTT 
    TTGCTAGAAG GAGGCAAAAC AGAGCAAGTA AAACATTCAG AGACATATTG CATGTTTCAA
    GACAAGAAGT ACAGAGTGGG TGAGAGATGG CATCCTTACC TGGAACCTTA TGGGTTGGTT
    TACTGCGTGA ACTGCATCTG CTCAGAGAAT GGGAATGTGC TTTGCAGCCG AGTCAGATGT
    CCAAATGTTC ATTGCCTTTC TCCTGTGCAT ATTCCTCATC TGTGCTGCCC TCGCTGCCCA
    GAAGACTCCT TACCCCCAGT GAACAATAAG GTGACCAGCA AGTCTTGCGA GTACAATGGG
    ACAACTTACC AACATGGAGA GCTGTTCGTA GCTGAAGGGC TCTTTCAGAA TCGGCAACCC
    AATCAATGCA CCCAGTGCAG CTGTTCGGAG GGAAACGTGT ATTGTGGTCT CAAGACTTGC
    CCCAAATTAA CCTGTGCCTT CCCAGTCTCT GTTCCAGATT CCTGCTGCCG GGTATGCAGA
    GGAGATGGAG AACTGTCATG GGAACATTCT GATGGTGATA TCTTCCGGCA ACCTGCCAAC
    AGAGAAGCAA GACATTCTTA CCACCGCTCT CACTATGATC CTCCACCAAG CCGACAGGCT
    GGAGGTCTGT CCCGCTTTCC TGGGGCCAGA AGTCACCGGG GAGCTCTTAT GGATTCCCAG
    CAAGCATCAG GAACCATTGT GCAAATTGTC ATCAATAACA AACACAAGCA TGGACAAGTG
    TGTGTTTCCA ATGGAAAGAC CTATTCTCAT GGCGAGTCCT GGCACCCAAA CCTCCGGGCA
    TTTGGCATTG TGGAGTGTGT GCTATGTACT TGTAATGTCA CCAAGCAAGA GTGTAAGAAA
    ATCCACTGCC CCAATCGATA CCCCTGCAAG TATCCTCAAA AAATAGACGG AAAATGCTGC
    AAGGTGTGTC CAGAACTTCC AGGCCAAAGC TTTGACAATA AAGGCTACTT CTGCGGGGAA
    GAAACGATGC CTGTGTATGA GTCTGTATTC ATGGAGGATG GGGAGACAAC CAGAAAAATA
    GCACTGGAGA CTGAGAGACC ACCTCAGGTA GAGGTCCACG TTTGGACTAT TCGAAAGGGC
    ATTCTCCAGC ACTTCCATAT TGAGAAGATC TCCAAGAGGA TGTTTGAGGA GCTTCCTCAC
    TTCAAGCTGG TGACCAGAAC AACCCTGAGC CAGTGGAAGA TCTTCACCGA AGGAGAAGCT
    CAGATCAGCC AGATGTGTTC AAGTCGTGTA TGCAGAACAG AGCTTGAAGA TTTAGTCAAG
    GTTTTGTACC TGGAGAGATC TGAAAAGGGC CACTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.