RTCA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RTCA
Entrez Gene ID	8634
Full Name	RNA 3'-terminal phosphate cyclase
Synonyms	RPC,RTC1,RTCD1
General protein information	Preferred Names RNA 3'-terminal phosphate cyclase Names RNA 3'-terminal phosphate cyclase RNA terminal phosphate cyclase domain 1 RNA terminal phosphate cyclase domain-containing protein 1 RTC domain-containing protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p21.2
Summary	This gene encodes a member of the RNA 3'-phosphate cyclase family. The encoded protein plays a role in RNA metabolism by catalyzing the ATP-dependent conversion of the 3'-phosphate of RNA substrates to a 2',3'-cyclic phosphodiester. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012].
Disorder MIM:	611286

mRNA and Protein(s)

mRNA	Protein	Name
XM_005271298.4	XP_005271355.1	RNA 3'-terminal phosphate cyclase isoform X1
NM_001130841.1	NP_001124313.1	RNA 3'-terminal phosphate cyclase isoform a
XM_005271298.5	XP_005271355.1	RNA 3'-terminal phosphate cyclase isoform X1
XM_005271299.4	XP_005271356.1	RNA 3'-terminal phosphate cyclase isoform X1
NM_003729.3	NP_003720.1	RNA 3'-terminal phosphate cyclase isoform b
XM_017002669.1	XP_016858158.1	RNA 3'-terminal phosphate cyclase isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	rtca	NP_955830.1
Drosophila melanogaster (fruit fly)	CG4061	NP_569977.1
	RTCD1	XP_001107617.1
Canis lupus familiaris (dog)	RTCA	XP_537054.2
Rattus norvegicus (Norway rat)	Rtcd1	NP_001004227.1
Xenopus tropicalis (tropical clawed frog)	rtca	NP_001120256.2
Homo sapiens (human)	RTCA	NP_001124313.1
Pan troglodytes (chimpanzee)	RTCD1	XP_001161113.1
Mus musculus (house mouse)	Rtca	NP_079793.2
Gallus gallus (chicken)	RTCA	XP_422311.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004820	XP_317988.3
Bos taurus (cattle)	RTCA	NP_001039520.1

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Structure-activity relationships in human RNA 3'-phosphate cyclase.
Tanaka N, Shuman S
RNA (New York, N.Y.)15(10)1865-74(2009 Oct)

The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea.
Genschik P, Billy E, Swianiewicz M, Filipowicz W
The EMBO journal16(10)2955-67(1997 May)

RNA 3'-terminal phosphate cyclase from HeLa cells.
Filipowicz W, Vicente O
Methods in enzymology181499-510(1990)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rtc1 forms a stable binary complex with a 3'-phosphate terminated RNA, but not with an identical 3'-OH terminated RNA
Title: Structure-activity relationships in human RNA 3'-phosphate cyclase.

The following RTCA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RTCA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07084	NM_001130841.1 Latest version!	Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu30753	NM_003729.3 Latest version!	Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu33942	XM_005271299.4 Latest version!	Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu33942	XM_017002669.1 Latest version!	Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu33942	XM_005271298.5 Latest version!	Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
View Old Accession Versions >>
OHu33942	XM_005271298.4	Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RTCA ( NM_001130841.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124313.1 Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu07084
Clone ID Related Accession (Same CDS sequence)	NM_001130841.1
Accession Version	NM_001130841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	RNA 3'-terminal phosphate cyclase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU310497.1, DA361597.1, BC012604.1, AL445928.8, CF141820.1, CA437545.1, AL663111.8, DB576272.1 and AI587400.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1803616.161967.1, SRR1660805.108705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGC CGCGGGTGGA GGTCGATGGC AGCATCATGG AAGGGGGCGG CCAGATCCTG 
AGAGTCTCTA CGGCCTTGAG CTGTCTCCTA GGCCTCCCCT TGCGGGTGCA GAAGATCCGA 
GCCGGCCGGA GCACGCCAGG CCTGAGTTCT GGAGGCTGGA AGTCCAAGAT CAAGGTCCTG 
ACAAGGCCTC AACATTTATC TGGACTGGAA ATGATTCGAG ATTTGTGTGA TGGGCAACTG 
GAGGGGGCAG AAATTGGCTC AACAGAAATA ACCTTTACAC CAGAGAAGAT CAAAGGTGGA 
ATCCACACAG CAGATACCAA GACAGCAGGG AGTGTGTGCC TCTTGATGCA GGTCTCAATG 
CCGTGTGTTC TCTTTGCTGC TTCTCCATCA GAACTTCATT TGAAAGGTGG AACTAATGCT 
GAAATGGCAC CACAGATCGA TTATACAGTG ATGGTCTTCA AGCCAATTGT TGAAAAATTT 
GGTTTCATAT TTAATTGTGA CATTAAAACA AGGGGATATT ACCCAAAAGG GGGTGGTGAA 
GTGATTGTTC GAATGTCACC AGTTAAACAA TTGAACCCTA TAAATTTAAC TGAGCGTGGC 
TGTGTGACTA AGATATATGG AAGAGCTTTC GTTGCTGGTG TTTTGCCATT TAAAGTAGCA 
AAAGATATGG CAGCGGCAGC AGTTAGATGC ATCAGAAAGG AGATCCGGGA TTTGTATGTT 
AACATCCAGC CTGTTCAAGA ACCTAAAGAC CAAGCATTTG GCAATGGAAA TGGAATAATA 
ATTATTGCTG AGACCTCCAC TGGCTGTTTG TTTGCTGGAT CATCGCTTGG TAAACGAGGT 
GTAAATGCAG ACAAAGTTGG AATTGAAGCT GCCGAAATGC TATTAGCAAA TCTTAGACAT 
GGTGGTACTG TGGATGAGTA TCTGCAAGAC CAGCTGATTG TTTTCATGGC ATTAGCCAAT 
GGAGTTTCCA GAATAAAAAC AGGACCAGTT ACACTCCATA CGCAAACCGC GATACATTTT 
GCTGAACAAA TAGCAAAGGC TAAATTTATT GTGAAGAAAT CAGAAGATGA AGAAGACGCC 
GCTAAAGATA CTTATATTAT TGAATGCCAA GGAATTGGGA TGACAAATCC AAATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124313.1
CDS	219..1358
Translation	MAGPRVEVDGSIMEGGGQILRVSTALSCLLGLPLRVQKIRAGRSTPGLSSGGWKSKIKVLTRPQHLSGLEMIRDLCDGQLEGAEIGSTEITFTPEKIKGGIHTADTKTAGSVCLLMQVSMPCVLFAASPSELHLKGGTNAEMAPQIDYTVMVFKPIVEKFGFIFNCDIKTRGYYPKGGGEVIVRMSPVKQLNPINLTERGCVTKIYGRAFVAGVLPFKVAKDMAAAAVRCIRKEIRDLYVNIQPVQEPKDQAFGNGNGIIIIAETSTGCLFAGSSLGKRGVNADKVGIEAAEMLLANLRHGGTVDEYLQDQLIVFMALANGVSRIKTGPVTLHTQTAIHFAEQIAKAKFIVKKSEDEEDAAKDTYIIECQGIGMTNPNL

Target ORF information:

RefSeq Version	NM_001130841.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130841.1

ORF Insert Sequence:

ATGGCGGGGC CGCGGGTGGA GGTCGATGGC AGCATCATGG AAGGGGGCGG CCAGATCCTG 
AGAGTCTCTA CGGCCTTGAG CTGTCTCCTA GGCCTCCCCT TGCGGGTGCA GAAGATCCGA 
GCCGGCCGGA GCACGCCAGG CCTGAGTTCT GGAGGCTGGA AGTCCAAGAT CAAGGTCCTG 
ACAAGGCCTC AACATTTATC TGGACTGGAA ATGATTCGAG ATTTGTGTGA TGGGCAACTG 
GAGGGGGCAG AAATTGGCTC AACAGAAATA ACCTTTACAC CAGAGAAGAT CAAAGGTGGA 
ATCCACACAG CAGATACCAA GACAGCAGGG AGTGTGTGCC TCTTGATGCA GGTCTCAATG 
CCGTGTGTTC TCTTTGCTGC TTCTCCATCA GAACTTCATT TGAAAGGTGG AACTAATGCT 
GAAATGGCAC CACAGATCGA TTATACAGTG ATGGTCTTCA AGCCAATTGT TGAAAAATTT 
GGTTTCATAT TTAATTGTGA CATTAAAACA AGGGGATATT ACCCAAAAGG GGGTGGTGAA 
GTGATTGTTC GAATGTCACC AGTTAAACAA TTGAACCCTA TAAATTTAAC TGAGCGTGGC 
TGTGTGACTA AGATATATGG AAGAGCTTTC GTTGCTGGTG TTTTGCCATT TAAAGTAGCA 
AAAGATATGG CAGCGGCAGC AGTTAGATGC ATCAGAAAGG AGATCCGGGA TTTGTATGTT 
AACATCCAGC CTGTTCAAGA ACCTAAAGAC CAAGCATTTG GCAATGGAAA TGGAATAATA 
ATTATTGCTG AGACCTCCAC TGGCTGTTTG TTTGCTGGAT CATCGCTTGG TAAACGAGGT 
GTAAATGCAG ACAAAGTTGG AATTGAAGCT GCCGAAATGC TATTAGCAAA TCTTAGACAT 
GGTGGTACTG TGGATGAGTA TCTGCAAGAC CAGCTGATTG TTTTCATGGC ATTAGCCAAT 
GGAGTTTCCA GAATAAAAAC AGGACCAGTT ACACTCCATA CGCAAACCGC GATACATTTT 
GCTGAACAAA TAGCAAAGGC TAAATTTATT GTGAAGAAAT CAGAAGATGA AGAAGACGCC 
GCTAAAGATA CTTATATTAT TGAATGCCAA GGAATTGGGA TGACAAATCC AAATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTCA ( NM_003729.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003720.1 Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu30753
Clone ID Related Accession (Same CDS sequence)	NM_003729.3
Accession Version	NM_003729.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	RNA 3'-terminal phosphate cyclase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU310497.1, DA361597.1, Y11651.1, CF141820.1, CA437545.1, AL663111.8, DB576272.1 and AI587400.1. On Aug 6, 2008 this sequence version replaced NM_003729.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.188624.1, SRR1660805.202065.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGC CGCGGGTGGA GGTCGATGGC AGCATCATGG AAGGGGGCGG CCAGATCCTG 
AGAGTCTCTA CGGCCTTGAG CTGTCTCCTA GGCCTCCCCT TGCGGGTGCA GAAGATCCGA 
GCCGGCCGGA GCACGCCAGG CCTGAGGCCT CAACATTTAT CTGGACTGGA AATGATTCGA 
GATTTGTGTG ATGGGCAACT GGAGGGGGCA GAAATTGGCT CAACAGAAAT AACCTTTACA 
CCAGAGAAGA TCAAAGGTGG AATCCACACA GCAGATACCA AGACAGCAGG GAGTGTGTGC 
CTCTTGATGC AGGTCTCAAT GCCGTGTGTT CTCTTTGCTG CTTCTCCATC AGAACTTCAT 
TTGAAAGGTG GAACTAATGC TGAAATGGCA CCACAGATCG ATTATACAGT GATGGTCTTC 
AAGCCAATTG TTGAAAAATT TGGTTTCATA TTTAATTGTG ACATTAAAAC AAGGGGATAT 
TACCCAAAAG GGGGTGGTGA AGTGATTGTT CGAATGTCAC CAGTTAAACA ATTGAACCCT 
ATAAATTTAA CTGAGCGTGG CTGTGTGACT AAGATATATG GAAGAGCTTT CGTTGCTGGT 
GTTTTGCCAT TTAAAGTAGC AAAAGATATG GCAGCGGCAG CAGTTAGATG CATCAGAAAG 
GAGATCCGGG ATTTGTATGT TAACATCCAG CCTGTTCAAG AACCTAAAGA CCAAGCATTT 
GGCAATGGAA ATGGAATAAT AATTATTGCT GAGACCTCCA CTGGCTGTTT GTTTGCTGGA 
TCATCGCTTG GTAAACGAGG TGTAAATGCA GACAAAGTTG GAATTGAAGC TGCCGAAATG 
CTATTAGCAA ATCTTAGACA TGGTGGTACT GTGGATGAGT ATCTGCAAGA CCAGCTGATT 
GTTTTCATGG CATTAGCCAA TGGAGTTTCC AGAATAAAAA CAGGACCAGT TACACTCCAT 
ACGCAAACCG CGATACATTT TGCTGAACAA ATAGCAAAGG CTAAATTTAT TGTGAAGAAA 
TCAGAAGATG AAGAAGACGC CGCTAAAGAT ACTTATATTA TTGAATGCCA AGGAATTGGG 
ATGACAAATC CAAATCTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003720.1
CDS	219..1319
Translation	MAGPRVEVDGSIMEGGGQILRVSTALSCLLGLPLRVQKIRAGRSTPGLRPQHLSGLEMIRDLCDGQLEGAEIGSTEITFTPEKIKGGIHTADTKTAGSVCLLMQVSMPCVLFAASPSELHLKGGTNAEMAPQIDYTVMVFKPIVEKFGFIFNCDIKTRGYYPKGGGEVIVRMSPVKQLNPINLTERGCVTKIYGRAFVAGVLPFKVAKDMAAAAVRCIRKEIRDLYVNIQPVQEPKDQAFGNGNGIIIIAETSTGCLFAGSSLGKRGVNADKVGIEAAEMLLANLRHGGTVDEYLQDQLIVFMALANGVSRIKTGPVTLHTQTAIHFAEQIAKAKFIVKKSEDEEDAAKDTYIIECQGIGMTNPNL

Target ORF information:

RefSeq Version	NM_003729.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003729.3

ORF Insert Sequence:

ATGGCGGGGC CGCGGGTGGA GGTCGATGGC AGCATCATGG AAGGGGGCGG CCAGATCCTG 
AGAGTCTCTA CGGCCTTGAG CTGTCTCCTA GGCCTCCCCT TGCGGGTGCA GAAGATCCGA 
GCCGGCCGGA GCACGCCAGG CCTGAGGCCT CAACATTTAT CTGGACTGGA AATGATTCGA 
GATTTGTGTG ATGGGCAACT GGAGGGGGCA GAAATTGGCT CAACAGAAAT AACCTTTACA 
CCAGAGAAGA TCAAAGGTGG AATCCACACA GCAGATACCA AGACAGCAGG GAGTGTGTGC 
CTCTTGATGC AGGTCTCAAT GCCGTGTGTT CTCTTTGCTG CTTCTCCATC AGAACTTCAT 
TTGAAAGGTG GAACTAATGC TGAAATGGCA CCACAGATCG ATTATACAGT GATGGTCTTC 
AAGCCAATTG TTGAAAAATT TGGTTTCATA TTTAATTGTG ACATTAAAAC AAGGGGATAT 
TACCCAAAAG GGGGTGGTGA AGTGATTGTT CGAATGTCAC CAGTTAAACA ATTGAACCCT 
ATAAATTTAA CTGAGCGTGG CTGTGTGACT AAGATATATG GAAGAGCTTT CGTTGCTGGT 
GTTTTGCCAT TTAAAGTAGC AAAAGATATG GCAGCGGCAG CAGTTAGATG CATCAGAAAG 
GAGATCCGGG ATTTGTATGT TAACATCCAG CCTGTTCAAG AACCTAAAGA CCAAGCATTT 
GGCAATGGAA ATGGAATAAT AATTATTGCT GAGACCTCCA CTGGCTGTTT GTTTGCTGGA 
TCATCGCTTG GTAAACGAGG TGTAAATGCA GACAAAGTTG GAATTGAAGC TGCCGAAATG 
CTATTAGCAA ATCTTAGACA TGGTGGTACT GTGGATGAGT ATCTGCAAGA CCAGCTGATT 
GTTTTCATGG CATTAGCCAA TGGAGTTTCC AGAATAAAAA CAGGACCAGT TACACTCCAT 
ACGCAAACCG CGATACATTT TGCTGAACAA ATAGCAAAGG CTAAATTTAT TGTGAAGAAA 
TCAGAAGATG AAGAAGACGC CGCTAAAGAT ACTTATATTA TTGAATGCCA AGGAATTGGG 
ATGACAAATC CAAATCTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTCA ( XM_005271298.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271355.1 Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu33942
Clone ID Related Accession (Same CDS sequence)	XM_005271298.4 , XM_005271298.5 , XM_005271299.4 , XM_017002669.1
Accession Version	XM_005271298.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	RNA 3'-terminal phosphate cyclase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005271298.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCGAG ATTTGTGTGA TGGGCAACTG GAGGGGGCAG AAATTGGCTC AACAGAAATA 
ACCTTTACAC CAGAGAAGAT CAAAGGTGGA ATCCACACAG CAGATACCAA GACAGCAGGG 
AGTGTGTGCC TCTTGATGCA GGTCTCAATG CCGTGTGTTC TCTTTGCTGC TTCTCCATCA 
GAACTTCATT TGAAAGGTGG AACTAATGCT GAAATGGCAC CACAGATCGA TTATACAGTG 
ATGGTCTTCA AGCCAATTGT TGAAAAATTT GGTTTCATAT TTAATTGTGA CATTAAAACA 
AGGGGATATT ACCCAAAAGG GGGTGGTGAA GTGATTGTTC GAATGTCACC AGTTAAACAA 
TTGAACCCTA TAAATTTAAC TGAGCGTGGC TGTGTGACTA AGATATATGG AAGAGCTTTC 
GTTGCTGGTG TTTTGCCATT TAAAGTAGCA AAAGATATGG CAGCGGCAGC AGTTAGATGC 
ATCAGAAAGG AGATCCGGGA TTTGTATGTT AACATCCAGC CTGTTCAAGA ACCTAAAGAC 
CAAGCATTTG GCAATGGAAA TGGAATAATA ATTATTGCTG AGACCTCCAC TGGCTGTTTG 
TTTGCTGGAT CATCGCTTGG TAAACGAGGT GTAAATGCAG ACAAAGTTGG AATTGAAGCT 
GCCGAAATGC TATTAGCAAA TCTTAGACAT GGTGGTACTG TGGATGAGTA TCTGCAAGAC 
CAGCTGATTG TTTTCATGGC ATTAGCCAAT GGAGTTTCCA GAATAAAAAC AGGACCAGTT 
ACACTCCATA CGCAAACCGC GATACATTTT GCTGAACAAA TAGCAAAGGC TAAATTTATT 
GTGAAGAAAT CAGAAGATGA AGAAGACGCC GCTAAAGATA CTTATATTAT TGAATGCCAA 
GGAATTGGGA TGACAAATCC AAATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271355.1
CDS	387..1316
Translation	MIRDLCDGQLEGAEIGSTEITFTPEKIKGGIHTADTKTAGSVCLLMQVSMPCVLFAASPSELHLKGGTNAEMAPQIDYTVMVFKPIVEKFGFIFNCDIKTRGYYPKGGGEVIVRMSPVKQLNPINLTERGCVTKIYGRAFVAGVLPFKVAKDMAAAAVRCIRKEIRDLYVNIQPVQEPKDQAFGNGNGIIIIAETSTGCLFAGSSLGKRGVNADKVGIEAAEMLLANLRHGGTVDEYLQDQLIVFMALANGVSRIKTGPVTLHTQTAIHFAEQIAKAKFIVKKSEDEEDAAKDTYIIECQGIGMTNPNL

Target ORF information:

RefSeq Version	XM_005271298.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271298.4

ORF Insert Sequence:

ATGATTCGAG ATTTGTGTGA TGGGCAACTG GAGGGGGCAG AAATTGGCTC AACAGAAATA 
ACCTTTACAC CAGAGAAGAT CAAAGGTGGA ATCCACACAG CAGATACCAA GACAGCAGGG 
AGTGTGTGCC TCTTGATGCA GGTCTCAATG CCGTGTGTTC TCTTTGCTGC TTCTCCATCA 
GAACTTCATT TGAAAGGTGG AACTAATGCT GAAATGGCAC CACAGATCGA TTATACAGTG 
ATGGTCTTCA AGCCAATTGT TGAAAAATTT GGTTTCATAT TTAATTGTGA CATTAAAACA 
AGGGGATATT ACCCAAAAGG GGGTGGTGAA GTGATTGTTC GAATGTCACC AGTTAAACAA 
TTGAACCCTA TAAATTTAAC TGAGCGTGGC TGTGTGACTA AGATATATGG AAGAGCTTTC 
GTTGCTGGTG TTTTGCCATT TAAAGTAGCA AAAGATATGG CAGCGGCAGC AGTTAGATGC 
ATCAGAAAGG AGATCCGGGA TTTGTATGTT AACATCCAGC CTGTTCAAGA ACCTAAAGAC 
CAAGCATTTG GCAATGGAAA TGGAATAATA ATTATTGCTG AGACCTCCAC TGGCTGTTTG 
TTTGCTGGAT CATCGCTTGG TAAACGAGGT GTAAATGCAG ACAAAGTTGG AATTGAAGCT 
GCCGAAATGC TATTAGCAAA TCTTAGACAT GGTGGTACTG TGGATGAGTA TCTGCAAGAC 
CAGCTGATTG TTTTCATGGC ATTAGCCAAT GGAGTTTCCA GAATAAAAAC AGGACCAGTT 
ACACTCCATA CGCAAACCGC GATACATTTT GCTGAACAAA TAGCAAAGGC TAAATTTATT 
GTGAAGAAAT CAGAAGATGA AGAAGACGCC GCTAAAGATA CTTATATTAT TGAATGCCAA 
GGAATTGGGA TGACAAATCC AAATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTCA ( XM_005271298.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271355.1 Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu33942
Clone ID Related Accession (Same CDS sequence)	XM_005271298.4 , XM_005271298.5 , XM_005271299.4 , XM_017002669.1
Accession Version	XM_005271298.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	RNA 3'-terminal phosphate cyclase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005271298.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCGAG ATTTGTGTGA TGGGCAACTG GAGGGGGCAG AAATTGGCTC AACAGAAATA 
ACCTTTACAC CAGAGAAGAT CAAAGGTGGA ATCCACACAG CAGATACCAA GACAGCAGGG 
AGTGTGTGCC TCTTGATGCA GGTCTCAATG CCGTGTGTTC TCTTTGCTGC TTCTCCATCA 
GAACTTCATT TGAAAGGTGG AACTAATGCT GAAATGGCAC CACAGATCGA TTATACAGTG 
ATGGTCTTCA AGCCAATTGT TGAAAAATTT GGTTTCATAT TTAATTGTGA CATTAAAACA 
AGGGGATATT ACCCAAAAGG GGGTGGTGAA GTGATTGTTC GAATGTCACC AGTTAAACAA 
TTGAACCCTA TAAATTTAAC TGAGCGTGGC TGTGTGACTA AGATATATGG AAGAGCTTTC 
GTTGCTGGTG TTTTGCCATT TAAAGTAGCA AAAGATATGG CAGCGGCAGC AGTTAGATGC 
ATCAGAAAGG AGATCCGGGA TTTGTATGTT AACATCCAGC CTGTTCAAGA ACCTAAAGAC 
CAAGCATTTG GCAATGGAAA TGGAATAATA ATTATTGCTG AGACCTCCAC TGGCTGTTTG 
TTTGCTGGAT CATCGCTTGG TAAACGAGGT GTAAATGCAG ACAAAGTTGG AATTGAAGCT 
GCCGAAATGC TATTAGCAAA TCTTAGACAT GGTGGTACTG TGGATGAGTA TCTGCAAGAC 
CAGCTGATTG TTTTCATGGC ATTAGCCAAT GGAGTTTCCA GAATAAAAAC AGGACCAGTT 
ACACTCCATA CGCAAACCGC GATACATTTT GCTGAACAAA TAGCAAAGGC TAAATTTATT 
GTGAAGAAAT CAGAAGATGA AGAAGACGCC GCTAAAGATA CTTATATTAT TGAATGCCAA 
GGAATTGGGA TGACAAATCC AAATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271355.1
CDS	390..1319
Translation	MIRDLCDGQLEGAEIGSTEITFTPEKIKGGIHTADTKTAGSVCLLMQVSMPCVLFAASPSELHLKGGTNAEMAPQIDYTVMVFKPIVEKFGFIFNCDIKTRGYYPKGGGEVIVRMSPVKQLNPINLTERGCVTKIYGRAFVAGVLPFKVAKDMAAAAVRCIRKEIRDLYVNIQPVQEPKDQAFGNGNGIIIIAETSTGCLFAGSSLGKRGVNADKVGIEAAEMLLANLRHGGTVDEYLQDQLIVFMALANGVSRIKTGPVTLHTQTAIHFAEQIAKAKFIVKKSEDEEDAAKDTYIIECQGIGMTNPNL

Target ORF information:

RefSeq Version	XM_005271298.5
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271298.5

ORF Insert Sequence:

ATGATTCGAG ATTTGTGTGA TGGGCAACTG GAGGGGGCAG AAATTGGCTC AACAGAAATA 
ACCTTTACAC CAGAGAAGAT CAAAGGTGGA ATCCACACAG CAGATACCAA GACAGCAGGG 
AGTGTGTGCC TCTTGATGCA GGTCTCAATG CCGTGTGTTC TCTTTGCTGC TTCTCCATCA 
GAACTTCATT TGAAAGGTGG AACTAATGCT GAAATGGCAC CACAGATCGA TTATACAGTG 
ATGGTCTTCA AGCCAATTGT TGAAAAATTT GGTTTCATAT TTAATTGTGA CATTAAAACA 
AGGGGATATT ACCCAAAAGG GGGTGGTGAA GTGATTGTTC GAATGTCACC AGTTAAACAA 
TTGAACCCTA TAAATTTAAC TGAGCGTGGC TGTGTGACTA AGATATATGG AAGAGCTTTC 
GTTGCTGGTG TTTTGCCATT TAAAGTAGCA AAAGATATGG CAGCGGCAGC AGTTAGATGC 
ATCAGAAAGG AGATCCGGGA TTTGTATGTT AACATCCAGC CTGTTCAAGA ACCTAAAGAC 
CAAGCATTTG GCAATGGAAA TGGAATAATA ATTATTGCTG AGACCTCCAC TGGCTGTTTG 
TTTGCTGGAT CATCGCTTGG TAAACGAGGT GTAAATGCAG ACAAAGTTGG AATTGAAGCT 
GCCGAAATGC TATTAGCAAA TCTTAGACAT GGTGGTACTG TGGATGAGTA TCTGCAAGAC 
CAGCTGATTG TTTTCATGGC ATTAGCCAAT GGAGTTTCCA GAATAAAAAC AGGACCAGTT 
ACACTCCATA CGCAAACCGC GATACATTTT GCTGAACAAA TAGCAAAGGC TAAATTTATT 
GTGAAGAAAT CAGAAGATGA AGAAGACGCC GCTAAAGATA CTTATATTAT TGAATGCCAA 
GGAATTGGGA TGACAAATCC AAATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTCA ( XM_005271299.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271356.1 Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu33942
Clone ID Related Accession (Same CDS sequence)	XM_005271298.4 , XM_005271298.5 , XM_005271299.4 , XM_017002669.1
Accession Version	XM_005271299.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	RNA 3'-terminal phosphate cyclase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005271299.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCGAG ATTTGTGTGA TGGGCAACTG GAGGGGGCAG AAATTGGCTC AACAGAAATA 
ACCTTTACAC CAGAGAAGAT CAAAGGTGGA ATCCACACAG CAGATACCAA GACAGCAGGG 
AGTGTGTGCC TCTTGATGCA GGTCTCAATG CCGTGTGTTC TCTTTGCTGC TTCTCCATCA 
GAACTTCATT TGAAAGGTGG AACTAATGCT GAAATGGCAC CACAGATCGA TTATACAGTG 
ATGGTCTTCA AGCCAATTGT TGAAAAATTT GGTTTCATAT TTAATTGTGA CATTAAAACA 
AGGGGATATT ACCCAAAAGG GGGTGGTGAA GTGATTGTTC GAATGTCACC AGTTAAACAA 
TTGAACCCTA TAAATTTAAC TGAGCGTGGC TGTGTGACTA AGATATATGG AAGAGCTTTC 
GTTGCTGGTG TTTTGCCATT TAAAGTAGCA AAAGATATGG CAGCGGCAGC AGTTAGATGC 
ATCAGAAAGG AGATCCGGGA TTTGTATGTT AACATCCAGC CTGTTCAAGA ACCTAAAGAC 
CAAGCATTTG GCAATGGAAA TGGAATAATA ATTATTGCTG AGACCTCCAC TGGCTGTTTG 
TTTGCTGGAT CATCGCTTGG TAAACGAGGT GTAAATGCAG ACAAAGTTGG AATTGAAGCT 
GCCGAAATGC TATTAGCAAA TCTTAGACAT GGTGGTACTG TGGATGAGTA TCTGCAAGAC 
CAGCTGATTG TTTTCATGGC ATTAGCCAAT GGAGTTTCCA GAATAAAAAC AGGACCAGTT 
ACACTCCATA CGCAAACCGC GATACATTTT GCTGAACAAA TAGCAAAGGC TAAATTTATT 
GTGAAGAAAT CAGAAGATGA AGAAGACGCC GCTAAAGATA CTTATATTAT TGAATGCCAA 
GGAATTGGGA TGACAAATCC AAATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271356.1
CDS	376..1305
Translation	MIRDLCDGQLEGAEIGSTEITFTPEKIKGGIHTADTKTAGSVCLLMQVSMPCVLFAASPSELHLKGGTNAEMAPQIDYTVMVFKPIVEKFGFIFNCDIKTRGYYPKGGGEVIVRMSPVKQLNPINLTERGCVTKIYGRAFVAGVLPFKVAKDMAAAAVRCIRKEIRDLYVNIQPVQEPKDQAFGNGNGIIIIAETSTGCLFAGSSLGKRGVNADKVGIEAAEMLLANLRHGGTVDEYLQDQLIVFMALANGVSRIKTGPVTLHTQTAIHFAEQIAKAKFIVKKSEDEEDAAKDTYIIECQGIGMTNPNL

Target ORF information:

RefSeq Version	XM_005271299.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271299.4

ORF Insert Sequence:

ATGATTCGAG ATTTGTGTGA TGGGCAACTG GAGGGGGCAG AAATTGGCTC AACAGAAATA 
ACCTTTACAC CAGAGAAGAT CAAAGGTGGA ATCCACACAG CAGATACCAA GACAGCAGGG 
AGTGTGTGCC TCTTGATGCA GGTCTCAATG CCGTGTGTTC TCTTTGCTGC TTCTCCATCA 
GAACTTCATT TGAAAGGTGG AACTAATGCT GAAATGGCAC CACAGATCGA TTATACAGTG 
ATGGTCTTCA AGCCAATTGT TGAAAAATTT GGTTTCATAT TTAATTGTGA CATTAAAACA 
AGGGGATATT ACCCAAAAGG GGGTGGTGAA GTGATTGTTC GAATGTCACC AGTTAAACAA 
TTGAACCCTA TAAATTTAAC TGAGCGTGGC TGTGTGACTA AGATATATGG AAGAGCTTTC 
GTTGCTGGTG TTTTGCCATT TAAAGTAGCA AAAGATATGG CAGCGGCAGC AGTTAGATGC 
ATCAGAAAGG AGATCCGGGA TTTGTATGTT AACATCCAGC CTGTTCAAGA ACCTAAAGAC 
CAAGCATTTG GCAATGGAAA TGGAATAATA ATTATTGCTG AGACCTCCAC TGGCTGTTTG 
TTTGCTGGAT CATCGCTTGG TAAACGAGGT GTAAATGCAG ACAAAGTTGG AATTGAAGCT 
GCCGAAATGC TATTAGCAAA TCTTAGACAT GGTGGTACTG TGGATGAGTA TCTGCAAGAC 
CAGCTGATTG TTTTCATGGC ATTAGCCAAT GGAGTTTCCA GAATAAAAAC AGGACCAGTT 
ACACTCCATA CGCAAACCGC GATACATTTT GCTGAACAAA TAGCAAAGGC TAAATTTATT 
GTGAAGAAAT CAGAAGATGA AGAAGACGCC GCTAAAGATA CTTATATTAT TGAATGCCAA 
GGAATTGGGA TGACAAATCC AAATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTCA ( XM_017002669.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858158.1 Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu33942
Clone ID Related Accession (Same CDS sequence)	XM_005271298.4 , XM_005271298.5 , XM_005271299.4 , XM_017002669.1
Accession Version	XM_017002669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	RNA 3'-terminal phosphate cyclase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCGAG ATTTGTGTGA TGGGCAACTG GAGGGGGCAG AAATTGGCTC AACAGAAATA 
ACCTTTACAC CAGAGAAGAT CAAAGGTGGA ATCCACACAG CAGATACCAA GACAGCAGGG 
AGTGTGTGCC TCTTGATGCA GGTCTCAATG CCGTGTGTTC TCTTTGCTGC TTCTCCATCA 
GAACTTCATT TGAAAGGTGG AACTAATGCT GAAATGGCAC CACAGATCGA TTATACAGTG 
ATGGTCTTCA AGCCAATTGT TGAAAAATTT GGTTTCATAT TTAATTGTGA CATTAAAACA 
AGGGGATATT ACCCAAAAGG GGGTGGTGAA GTGATTGTTC GAATGTCACC AGTTAAACAA 
TTGAACCCTA TAAATTTAAC TGAGCGTGGC TGTGTGACTA AGATATATGG AAGAGCTTTC 
GTTGCTGGTG TTTTGCCATT TAAAGTAGCA AAAGATATGG CAGCGGCAGC AGTTAGATGC 
ATCAGAAAGG AGATCCGGGA TTTGTATGTT AACATCCAGC CTGTTCAAGA ACCTAAAGAC 
CAAGCATTTG GCAATGGAAA TGGAATAATA ATTATTGCTG AGACCTCCAC TGGCTGTTTG 
TTTGCTGGAT CATCGCTTGG TAAACGAGGT GTAAATGCAG ACAAAGTTGG AATTGAAGCT 
GCCGAAATGC TATTAGCAAA TCTTAGACAT GGTGGTACTG TGGATGAGTA TCTGCAAGAC 
CAGCTGATTG TTTTCATGGC ATTAGCCAAT GGAGTTTCCA GAATAAAAAC AGGACCAGTT 
ACACTCCATA CGCAAACCGC GATACATTTT GCTGAACAAA TAGCAAAGGC TAAATTTATT 
GTGAAGAAAT CAGAAGATGA AGAAGACGCC GCTAAAGATA CTTATATTAT TGAATGCCAA 
GGAATTGGGA TGACAAATCC AAATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858158.1
CDS	133..1062
Translation	MIRDLCDGQLEGAEIGSTEITFTPEKIKGGIHTADTKTAGSVCLLMQVSMPCVLFAASPSELHLKGGTNAEMAPQIDYTVMVFKPIVEKFGFIFNCDIKTRGYYPKGGGEVIVRMSPVKQLNPINLTERGCVTKIYGRAFVAGVLPFKVAKDMAAAAVRCIRKEIRDLYVNIQPVQEPKDQAFGNGNGIIIIAETSTGCLFAGSSLGKRGVNADKVGIEAAEMLLANLRHGGTVDEYLQDQLIVFMALANGVSRIKTGPVTLHTQTAIHFAEQIAKAKFIVKKSEDEEDAAKDTYIIECQGIGMTNPNL

Target ORF information:

RefSeq Version	XM_017002669.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA 3'-terminal phosphate cyclase (RTCA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002669.1

ORF Insert Sequence:

ATGATTCGAG ATTTGTGTGA TGGGCAACTG GAGGGGGCAG AAATTGGCTC AACAGAAATA 
ACCTTTACAC CAGAGAAGAT CAAAGGTGGA ATCCACACAG CAGATACCAA GACAGCAGGG 
AGTGTGTGCC TCTTGATGCA GGTCTCAATG CCGTGTGTTC TCTTTGCTGC TTCTCCATCA 
GAACTTCATT TGAAAGGTGG AACTAATGCT GAAATGGCAC CACAGATCGA TTATACAGTG 
ATGGTCTTCA AGCCAATTGT TGAAAAATTT GGTTTCATAT TTAATTGTGA CATTAAAACA 
AGGGGATATT ACCCAAAAGG GGGTGGTGAA GTGATTGTTC GAATGTCACC AGTTAAACAA 
TTGAACCCTA TAAATTTAAC TGAGCGTGGC TGTGTGACTA AGATATATGG AAGAGCTTTC 
GTTGCTGGTG TTTTGCCATT TAAAGTAGCA AAAGATATGG CAGCGGCAGC AGTTAGATGC 
ATCAGAAAGG AGATCCGGGA TTTGTATGTT AACATCCAGC CTGTTCAAGA ACCTAAAGAC 
CAAGCATTTG GCAATGGAAA TGGAATAATA ATTATTGCTG AGACCTCCAC TGGCTGTTTG 
TTTGCTGGAT CATCGCTTGG TAAACGAGGT GTAAATGCAG ACAAAGTTGG AATTGAAGCT 
GCCGAAATGC TATTAGCAAA TCTTAGACAT GGTGGTACTG TGGATGAGTA TCTGCAAGAC 
CAGCTGATTG TTTTCATGGC ATTAGCCAAT GGAGTTTCCA GAATAAAAAC AGGACCAGTT 
ACACTCCATA CGCAAACCGC GATACATTTT GCTGAACAAA TAGCAAAGGC TAAATTTATT 
GTGAAGAAAT CAGAAGATGA AGAAGACGCC GCTAAAGATA CTTATATTAT TGAATGCCAA 
GGAATTGGGA TGACAAATCC AAATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Structure-activity relationships in human RNA 3'-phosphate cyclase.
RNA (New York, N.Y.)15(10)1865-74(2009 Oct)
Tanaka N,Shuman S

The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea.
The EMBO journal16(10)2955-67(1997 May)
Genschik P,Billy E,Swianiewicz M,Filipowicz W

RNA 3'-terminal phosphate cyclase from HeLa cells.
Methods in enzymology181499-510(1990)
Filipowicz W,Vicente O

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RTCA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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FAX

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