ACSS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACSS1
Entrez Gene ID	84532
Full Name	acyl-CoA synthetase short chain family member 1
Synonyms	ACAS2L,ACECS1,AceCS2L
General protein information	Preferred Names acyl-CoA synthetase short chain family member 1 Names acetyl-coenzyme A synthetase 2-like, mitochondrial acetate--CoA ligase 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20p11.21
Summary	This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011].
Disorder MIM:	614355

mRNA and Protein(s)

mRNA	Protein	Name
NM_032501.3	NP_115890.2	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform 1 precursor
NM_001252676.1	NP_001239605.1	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform 3
XM_006723659.1	XP_006723722.1	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform X1
NM_032501.4	NP_115890.2	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform 1 precursor
NM_001252677.1	NP_001239606.1	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform 4 precursor
NM_001252675.1	NP_001239604.1	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform 2 precursor
XM_011529388.1	XP_011527690.1	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	acss1	NP_001074125.3
Homo sapiens (human)	ACSS1	NP_115890.2
Pan troglodytes (chimpanzee)	ACSS1	XP_001149851.1
	ACSS1	XP_001099300.2
Canis lupus familiaris (dog)	ACSS1	XP_534200.4
Bos taurus (cattle)	ACSS1	NP_777171.1
Mus musculus (house mouse)	Acss1	NP_542142.1
Gallus gallus (chicken)	ACSS1	XP_415011.4
Saccharomyces cerevisiae (baker's yeast)	ACS2	NP_013254.1
Neurospora crassa	NCU06836	XP_963677.2
Xenopus tropicalis (tropical clawed frog)	acss1	XP_002937499.1
Eremothecium gossypii	AGOS_AGL148C	NP_986519.1
Rattus norvegicus (Norway rat)	Acss1	NP_001099994.1
Kluyveromyces lactis	KLLA0D17336g	XP_453827.1
Schizosaccharomyces pombe (fission yeast)	SPCC191.02c	NP_588291.2
Magnaporthe oryzae (rice blast fungus)	MGG_03201	XP_003716783.1

Gene Ontology
Bibliography

Related articles in PubMed

Glucose-independent Acetate Metabolism Promotes Melanoma Cell Survival and Tumor Growth.
Lakhter AJ, Hamilton J, Konger RL, Brustovetsky N, Broxmeyer HE, Naidu SR
The Journal of biological chemistry291(42)21869-21879(2016 Oct)

Gene network analysis reveals a novel 22-gene signature of carbon metabolism in hepatocellular carcinoma.
Zhang J, Baddoo M, Han C, Strong MJ, Cvitanovic J, Moroz K, Dash S, Flemington EK, Wu T
Oncotarget7(31)49232-49245(2016 Aug)

A novel Acetyl-CoA synthetase short-chain subfamily member 1 (Acss1) gene indicates a dynamic history of paralogue retention and loss in vertebrates.
Castro LF, Lopes-Marques M, Wilson JM, Rocha E, Reis-Henriques MA, Santos MM, Cunha I
Gene497(2)249-55(2012 Apr)

Common genetic variation and performance on standardized cognitive tests.
Cirulli ET, Kasperavici?te D, Attix DK, Need AC, Ge D, Gibson G, Goldstein DB
European journal of human genetics : EJHG18(7)815-20(2010 Jul)

The importance of acetyl coenzyme A synthetase for 11C-acetate uptake and cell survival in hepatocellular carcinoma.
Yun M, Bang SH, Kim JW, Park JY, Kim KS, Lee JD
Journal of nuclear medicine : official publication, Society of Nuclear Medicine50(8)1222-8(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ACSS1 is essential for glucose-independent acetate-mediated cell survival and tumor growth.
Title: Glucose-independent Acetate Metabolism Promotes Melanoma Cell Survival and Tumor Growth.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association of genetic variants with hemorrhagic stroke in Japanese individuals.

Observational study of gene-disease association. (HuGE Navigator)
Title: Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals.

The following ACSS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACSS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09622	NM_032501.4 Latest version!	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu09598	NM_001252676.1 Latest version!	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu73567	XM_011529388.1 Latest version!	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu09617	NM_001252675.1 Latest version!	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu55918	NM_001252677.1 Latest version!	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu52816	XM_006723659.1 Latest version!	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu09622	NM_032501.3	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACSS1 ( NM_032501.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115890.2 Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09622
Clone ID Related Accession (Same CDS sequence)	NM_032501.3 , NM_032501.4
Accession Version	NM_032501.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Homo sapiens(human)
Product	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM150326.1, AK314440.1, AK125923.1 and AW024920.1. On Nov 26, 2011 this sequence version replaced NM_032501.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC039261.1, SRR1803613.146160.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC GCACCCTGGG CCGCGGCGTC GGGAGGCTGC TGGGCAGCCT GCGAGGGCTC 
TCGGGGCAGC CCGCGCGGCC GCCGTGCGGG GTGAGCGCGC CGCGCAGGGC GGCCTCGGGA 
CCCTCGGGCA GCGCTCCCGC AGTTGCAGCA GCAGCAGCAC AGCCAGGCTC GTATCCCGCG 
CTGAGTGCAC AGGCAGCCCG GGAGCCGGCC GCCTTCTGGG GGCCTCTGGC GCGGGACACT 
CTCGTGTGGG ACACCCCCTA CCACACCGTC TGGGACTGCG ACTTCAGCAC TGGCAAGATC 
GGCTGGTTCC TGGGAGGCCA GTTAAATGTC TCTGTCAACT GCTTGGACCA GCATGTTCGG 
AAGTCCCCCG AGAGCGTTGC TTTGATCTGG GAGCGCGATG AGCCTGGAAC GGAAGTGAGG 
ATCACCTACA GGGAACTACT GGAGACCACG TGCCGCCTGG CCAACACGCT GAAGAGGCAT 
GGAGTCCACC GTGGGGACCG TGTTGCCATC TACATGCCCG TGTCCCCATT GGCTGTGGCA 
GCAATGCTGG CCTGTGCCAG GATCGGAGCT GTCCACACAG TCATCTTTGC TGGCTTCAGT 
GCAGAGTCCT TGGCTGGGAG GATCAATGAT GCCAAGTGCA AGGTGGTTAT CACCTTCAAC 
CAAGGACTCC GGGGTGGGCG CGTGGTGGAG CTGAAGAAAA TAGTGGATGA GGCTGTGAAG 
CACTGCCCCA CCGTGCAGCA TGTCCTGGTG GCTCACAGGA CAGACAACAA GGTCCACATG 
GGGGATCTGG ACGTCCCGCT GGAGCAGGAA ATGGCCAAGG AGGACCCTGT TTGCGCCCCA 
GAGAGCATGG GCAGTGAGGA CATGCTCTTC ATGCTGTACA CCTCAGGGAG CACCGGAATG 
CCCAAGGGCA TCGTCCATAC CCAGGCAGGC TACCTGCTCT ATGCCGCCCT GACTCACAAG 
CTTGTGTTTG ACCACCAGCC AGGTGACATC TTTGGCTGTG TGGCCGACAT CGGTTGGATT 
ACAGGACACA GCTACGTGGT GTATGGGCCT CTCTGCAATG GTGCCACCAG CGTCCTTTTT 
GAGAGCACCC CAGTTTATCC CAATGCTGGT CGGTACTGGG AGACAGTAGA GAGGTTGAAG 
ATCAATCAGT TCTATGGCGC CCCAACGGCT GTCCGGCTGT TGCTGAAATA CGGTGATGCC 
TGGGTGAAGA AGTATGATCG CTCCTCCCTG CGGACCCTGG GGTCAGTGGG AGAGCCCATC 
AACTGTGAGG CCTGGGAGTG GCTTCACAGG GTGGTGGGGG ACAGCAGGTG CACGCTGGTG 
GACACCTGGT GGCAGACAGA AACAGGTGGC ATCTGCATCG CACCACGGCC CTCGGAAGAA 
GGGGCGGAAA TCCTCCCTGC CATGGCGATG AGGCCCTTCT TTGGCATCGT CCCCGTCCTC 
ATGGATGAGA AGGGCAGCGT CGTGGAGGGC AGCAACGTCT CCGGGGCCCT GTGCATCTCC 
CAGGCCTGGC CGGGCATGGC CAGGACCATC TATGGCGACC ACCAGCGATT TGTGGACGCC 
TACTTCAAGG CCTACCCAGG CTATTACTTC ACTGGAGACG GGGCTTACCG AACTGAGGGC 
GGCTATTACC AGATCACAGG GCGGATGGAT GATGTCATCA ACATCAGTGG CCACCGGCTG 
GGGACCGCAG AGATTGAGGA CGCCATCGCC GACCACCCTG CAGTACCAGA AAGTGCTGTC 
ATTGGCTACC CCCACGACAT CAAAGGAGAA GCTGCCTTTG CCTTCATTGT GGTGAAAGAT 
AGTGCGGGTG ACTCAGATGT GGTGGTGCAG GAGCTCAAGT CCATGGTGGC CACCAAGATC 
GCCAAATATG CTGTGCCTGA TGAGATCCTG GTGGTGAAAC GTCTTCCAAA AACCAGGTCT 
GGGAAGGTCA TGCGGCGGCT CCTGAGGAAG ATCATCACTA GTGAGGCCCA GGAGCTGGGA 
GACACTACCA CCTTGGAGGA CCCCAGCATC ATCGCAGAGA TCCTGAGTGT CTACCAGAAG 
TGCAAGGACA AGCAGGCTGC TGCTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115890.2
CDS	81..2150
Translation	MAARTLGRGVGRLLGSLRGLSGQPARPPCGVSAPRRAASGPSGSAPAVAAAAAQPGSYPALSAQAAREPAAFWGPLARDTLVWDTPYHTVWDCDFSTGKIGWFLGGQLNVSVNCLDQHVRKSPESVALIWERDEPGTEVRITYRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKHCPTVQHVLVAHRTDNKVHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK

Target ORF information:

RefSeq Version	NM_032501.3
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032501.3

ORF Insert Sequence:

ATGGCGGCGC GCACCCTGGG CCGCGGCGTC GGGAGGCTGC TGGGCAGCCT GCGAGGGCTC 
TCGGGGCAGC CCGCGCGGCC GCCGTGCGGG GTGAGCGCGC CGCGCAGGGC GGCCTCGGGA 
CCCTCGGGCA GCGCTCCCGC AGTTGCAGCA GCAGCAGCAC AGCCAGGCTC GTATCCCGCG 
CTGAGTGCAC AGGCAGCCCG GGAGCCGGCC GCCTTCTGGG GGCCTCTGGC GCGGGACACT 
CTCGTGTGGG ACACCCCCTA CCACACCGTC TGGGACTGCG ACTTCAGCAC TGGCAAGATC 
GGCTGGTTCC TGGGAGGCCA GTTAAATGTC TCTGTCAACT GCTTGGACCA GCATGTTCGG 
AAGTCCCCCG AGAGCGTTGC TTTGATCTGG GAGCGCGATG AGCCTGGAAC GGAAGTGAGG 
ATCACCTACA GGGAACTACT GGAGACCACG TGCCGCCTGG CCAACACGCT GAAGAGGCAT 
GGAGTCCACC GTGGGGACCG TGTTGCCATC TACATGCCCG TGTCCCCATT GGCTGTGGCA 
GCAATGCTGG CCTGTGCCAG GATCGGAGCT GTCCACACAG TCATCTTTGC TGGCTTCAGT 
GCAGAGTCCT TGGCTGGGAG GATCAATGAT GCCAAGTGCA AGGTGGTTAT CACCTTCAAC 
CAAGGACTCC GGGGTGGGCG CGTGGTGGAG CTGAAGAAAA TAGTGGATGA GGCTGTGAAG 
CACTGCCCCA CCGTGCAGCA TGTCCTGGTG GCTCACAGGA CAGACAACAA GGTCCACATG 
GGGGATCTGG ACGTCCCGCT GGAGCAGGAA ATGGCCAAGG AGGACCCTGT TTGCGCCCCA 
GAGAGCATGG GCAGTGAGGA CATGCTCTTC ATGCTGTACA CCTCAGGGAG CACCGGAATG 
CCCAAGGGCA TCGTCCATAC CCAGGCAGGC TACCTGCTCT ATGCCGCCCT GACTCACAAG 
CTTGTGTTTG ACCACCAGCC AGGTGACATC TTTGGCTGTG TGGCCGACAT CGGTTGGATT 
ACAGGACACA GCTACGTGGT GTATGGGCCT CTCTGCAATG GTGCCACCAG CGTCCTTTTT 
GAGAGCACCC CAGTTTATCC CAATGCTGGT CGGTACTGGG AGACAGTAGA GAGGTTGAAG 
ATCAATCAGT TCTATGGCGC CCCAACGGCT GTCCGGCTGT TGCTGAAATA CGGTGATGCC 
TGGGTGAAGA AGTATGATCG CTCCTCCCTG CGGACCCTGG GGTCAGTGGG AGAGCCCATC 
AACTGTGAGG CCTGGGAGTG GCTTCACAGG GTGGTGGGGG ACAGCAGGTG CACGCTGGTG 
GACACCTGGT GGCAGACAGA AACAGGTGGC ATCTGCATCG CACCACGGCC CTCGGAAGAA 
GGGGCGGAAA TCCTCCCTGC CATGGCGATG AGGCCCTTCT TTGGCATCGT CCCCGTCCTC 
ATGGATGAGA AGGGCAGCGT CGTGGAGGGC AGCAACGTCT CCGGGGCCCT GTGCATCTCC 
CAGGCCTGGC CGGGCATGGC CAGGACCATC TATGGCGACC ACCAGCGATT TGTGGACGCC 
TACTTCAAGG CCTACCCAGG CTATTACTTC ACTGGAGACG GGGCTTACCG AACTGAGGGC 
GGCTATTACC AGATCACAGG GCGGATGGAT GATGTCATCA ACATCAGTGG CCACCGGCTG 
GGGACCGCAG AGATTGAGGA CGCCATCGCC GACCACCCTG CAGTACCAGA AAGTGCTGTC 
ATTGGCTACC CCCACGACAT CAAAGGAGAA GCTGCCTTTG CCTTCATTGT GGTGAAAGAT 
AGTGCGGGTG ACTCAGATGT GGTGGTGCAG GAGCTCAAGT CCATGGTGGC CACCAAGATC 
GCCAAATATG CTGTGCCTGA TGAGATCCTG GTGGTGAAAC GTCTTCCAAA AACCAGGTCT 
GGGAAGGTCA TGCGGCGGCT CCTGAGGAAG ATCATCACTA GTGAGGCCCA GGAGCTGGGA 
GACACTACCA CCTTGGAGGA CCCCAGCATC ATCGCAGAGA TCCTGAGTGT CTACCAGAAG 
TGCAAGGACA AGCAGGCTGC TGCTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSS1 ( NM_032501.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_115890.2 Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09622
Clone ID Related Accession (Same CDS sequence)	NM_032501.3 , NM_032501.4
Accession Version	NM_032501.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-10
Organism	Homo sapiens(human)
Product	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM150326.1, AK314440.1 and AK125923.1. On Nov 22, 2018 this sequence version replaced NM_032501.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC039261.1, SRR1803613.146160.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000323482.9/ ENSP00000316924.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGC GCACCCTGGG CCGCGGCGTC GGGAGGCTGC TGGGCAGCCT GCGAGGGCTC 
TCGGGGCAGC CCGCGCGGCC GCCGTGCGGG GTGAGCGCGC CGCGCAGGGC GGCCTCGGGA 
CCCTCGGGCA GCGCTCCCGC AGTTGCAGCA GCAGCAGCAC AGCCAGGCTC GTATCCCGCG 
CTGAGTGCAC AGGCAGCCCG GGAGCCGGCC GCCTTCTGGG GGCCTCTGGC GCGGGACACT 
CTCGTGTGGG ACACCCCCTA CCACACCGTC TGGGACTGCG ACTTCAGCAC TGGCAAGATC 
GGCTGGTTCC TGGGAGGCCA GTTAAATGTC TCTGTCAACT GCTTGGACCA GCATGTTCGG 
AAGTCCCCCG AGAGCGTTGC TTTGATCTGG GAGCGCGATG AGCCTGGAAC GGAAGTGAGG 
ATCACCTACA GGGAACTACT GGAGACCACG TGCCGCCTGG CCAACACGCT GAAGAGGCAT 
GGAGTCCACC GTGGGGACCG TGTTGCCATC TACATGCCCG TGTCCCCATT GGCTGTGGCA 
GCAATGCTGG CCTGTGCCAG GATCGGAGCT GTCCACACAG TCATCTTTGC TGGCTTCAGT 
GCAGAGTCCT TGGCTGGGAG GATCAATGAT GCCAAGTGCA AGGTGGTTAT CACCTTCAAC 
CAAGGACTCC GGGGTGGGCG CGTGGTGGAG CTGAAGAAAA TAGTGGATGA GGCTGTGAAG 
CACTGCCCCA CCGTGCAGCA TGTCCTGGTG GCTCACAGGA CAGACAACAA GGTCCACATG 
GGGGATCTGG ACGTCCCGCT GGAGCAGGAA ATGGCCAAGG AGGACCCTGT TTGCGCCCCA 
GAGAGCATGG GCAGTGAGGA CATGCTCTTC ATGCTGTACA CCTCAGGGAG CACCGGAATG 
CCCAAGGGCA TCGTCCATAC CCAGGCAGGC TACCTGCTCT ATGCCGCCCT GACTCACAAG 
CTTGTGTTTG ACCACCAGCC AGGTGACATC TTTGGCTGTG TGGCCGACAT CGGTTGGATT 
ACAGGACACA GCTACGTGGT GTATGGGCCT CTCTGCAATG GTGCCACCAG CGTCCTTTTT 
GAGAGCACCC CAGTTTATCC CAATGCTGGT CGGTACTGGG AGACAGTAGA GAGGTTGAAG 
ATCAATCAGT TCTATGGCGC CCCAACGGCT GTCCGGCTGT TGCTGAAATA CGGTGATGCC 
TGGGTGAAGA AGTATGATCG CTCCTCCCTG CGGACCCTGG GGTCAGTGGG AGAGCCCATC 
AACTGTGAGG CCTGGGAGTG GCTTCACAGG GTGGTGGGGG ACAGCAGGTG CACGCTGGTG 
GACACCTGGT GGCAGACAGA AACAGGTGGC ATCTGCATCG CACCACGGCC CTCGGAAGAA 
GGGGCGGAAA TCCTCCCTGC CATGGCGATG AGGCCCTTCT TTGGCATCGT CCCCGTCCTC 
ATGGATGAGA AGGGCAGCGT CGTGGAGGGC AGCAACGTCT CCGGGGCCCT GTGCATCTCC 
CAGGCCTGGC CGGGCATGGC CAGGACCATC TATGGCGACC ACCAGCGATT TGTGGACGCC 
TACTTCAAGG CCTACCCAGG CTATTACTTC ACTGGAGACG GGGCTTACCG AACTGAGGGC 
GGCTATTACC AGATCACAGG GCGGATGGAT GATGTCATCA ACATCAGTGG CCACCGGCTG 
GGGACCGCAG AGATTGAGGA CGCCATCGCC GACCACCCTG CAGTACCAGA AAGTGCTGTC 
ATTGGCTACC CCCACGACAT CAAAGGAGAA GCTGCCTTTG CCTTCATTGT GGTGAAAGAT 
AGTGCGGGTG ACTCAGATGT GGTGGTGCAG GAGCTCAAGT CCATGGTGGC CACCAAGATC 
GCCAAATATG CTGTGCCTGA TGAGATCCTG GTGGTGAAAC GTCTTCCAAA AACCAGGTCT 
GGGAAGGTCA TGCGGCGGCT CCTGAGGAAG ATCATCACTA GTGAGGCCCA GGAGCTGGGA 
GACACTACCA CCTTGGAGGA CCCCAGCATC ATCGCAGAGA TCCTGAGTGT CTACCAGAAG 
TGCAAGGACA AGCAGGCTGC TGCTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115890.2
CDS	38..2107
Translation	MAARTLGRGVGRLLGSLRGLSGQPARPPCGVSAPRRAASGPSGSAPAVAAAAAQPGSYPALSAQAAREPAAFWGPLARDTLVWDTPYHTVWDCDFSTGKIGWFLGGQLNVSVNCLDQHVRKSPESVALIWERDEPGTEVRITYRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKHCPTVQHVLVAHRTDNKVHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK

Target ORF information:

RefSeq Version	NM_032501.4
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032501.4

ORF Insert Sequence:

ATGGCGGCGC GCACCCTGGG CCGCGGCGTC GGGAGGCTGC TGGGCAGCCT GCGAGGGCTC 
TCGGGGCAGC CCGCGCGGCC GCCGTGCGGG GTGAGCGCGC CGCGCAGGGC GGCCTCGGGA 
CCCTCGGGCA GCGCTCCCGC AGTTGCAGCA GCAGCAGCAC AGCCAGGCTC GTATCCCGCG 
CTGAGTGCAC AGGCAGCCCG GGAGCCGGCC GCCTTCTGGG GGCCTCTGGC GCGGGACACT 
CTCGTGTGGG ACACCCCCTA CCACACCGTC TGGGACTGCG ACTTCAGCAC TGGCAAGATC 
GGCTGGTTCC TGGGAGGCCA GTTAAATGTC TCTGTCAACT GCTTGGACCA GCATGTTCGG 
AAGTCCCCCG AGAGCGTTGC TTTGATCTGG GAGCGCGATG AGCCTGGAAC GGAAGTGAGG 
ATCACCTACA GGGAACTACT GGAGACCACG TGCCGCCTGG CCAACACGCT GAAGAGGCAT 
GGAGTCCACC GTGGGGACCG TGTTGCCATC TACATGCCCG TGTCCCCATT GGCTGTGGCA 
GCAATGCTGG CCTGTGCCAG GATCGGAGCT GTCCACACAG TCATCTTTGC TGGCTTCAGT 
GCAGAGTCCT TGGCTGGGAG GATCAATGAT GCCAAGTGCA AGGTGGTTAT CACCTTCAAC 
CAAGGACTCC GGGGTGGGCG CGTGGTGGAG CTGAAGAAAA TAGTGGATGA GGCTGTGAAG 
CACTGCCCCA CCGTGCAGCA TGTCCTGGTG GCTCACAGGA CAGACAACAA GGTCCACATG 
GGGGATCTGG ACGTCCCGCT GGAGCAGGAA ATGGCCAAGG AGGACCCTGT TTGCGCCCCA 
GAGAGCATGG GCAGTGAGGA CATGCTCTTC ATGCTGTACA CCTCAGGGAG CACCGGAATG 
CCCAAGGGCA TCGTCCATAC CCAGGCAGGC TACCTGCTCT ATGCCGCCCT GACTCACAAG 
CTTGTGTTTG ACCACCAGCC AGGTGACATC TTTGGCTGTG TGGCCGACAT CGGTTGGATT 
ACAGGACACA GCTACGTGGT GTATGGGCCT CTCTGCAATG GTGCCACCAG CGTCCTTTTT 
GAGAGCACCC CAGTTTATCC CAATGCTGGT CGGTACTGGG AGACAGTAGA GAGGTTGAAG 
ATCAATCAGT TCTATGGCGC CCCAACGGCT GTCCGGCTGT TGCTGAAATA CGGTGATGCC 
TGGGTGAAGA AGTATGATCG CTCCTCCCTG CGGACCCTGG GGTCAGTGGG AGAGCCCATC 
AACTGTGAGG CCTGGGAGTG GCTTCACAGG GTGGTGGGGG ACAGCAGGTG CACGCTGGTG 
GACACCTGGT GGCAGACAGA AACAGGTGGC ATCTGCATCG CACCACGGCC CTCGGAAGAA 
GGGGCGGAAA TCCTCCCTGC CATGGCGATG AGGCCCTTCT TTGGCATCGT CCCCGTCCTC 
ATGGATGAGA AGGGCAGCGT CGTGGAGGGC AGCAACGTCT CCGGGGCCCT GTGCATCTCC 
CAGGCCTGGC CGGGCATGGC CAGGACCATC TATGGCGACC ACCAGCGATT TGTGGACGCC 
TACTTCAAGG CCTACCCAGG CTATTACTTC ACTGGAGACG GGGCTTACCG AACTGAGGGC 
GGCTATTACC AGATCACAGG GCGGATGGAT GATGTCATCA ACATCAGTGG CCACCGGCTG 
GGGACCGCAG AGATTGAGGA CGCCATCGCC GACCACCCTG CAGTACCAGA AAGTGCTGTC 
ATTGGCTACC CCCACGACAT CAAAGGAGAA GCTGCCTTTG CCTTCATTGT GGTGAAAGAT 
AGTGCGGGTG ACTCAGATGT GGTGGTGCAG GAGCTCAAGT CCATGGTGGC CACCAAGATC 
GCCAAATATG CTGTGCCTGA TGAGATCCTG GTGGTGAAAC GTCTTCCAAA AACCAGGTCT 
GGGAAGGTCA TGCGGCGGCT CCTGAGGAAG ATCATCACTA GTGAGGCCCA GGAGCTGGGA 
GACACTACCA CCTTGGAGGA CCCCAGCATC ATCGCAGAGA TCCTGAGTGT CTACCAGAAG 
TGCAAGGACA AGCAGGCTGC TGCTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSS1 ( NM_001252676.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001239605.1 Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 3, mRNA.

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CloneID	OHu09598
Clone ID Related Accession (Same CDS sequence)	NM_001252676.1
Accession Version	NM_001252676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-10
Organism	Homo sapiens(human)
Product	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC404033.1, AK296306.1, AK314440.1, AK125923.1 and AW024920.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) is shorter and has a distinct N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK296306.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGCGCC GAAAGGAAAG ACAACCTTGG GATCGCTTCC ACTTCCTCCA CTTCGCACCG 
CATGGCCGGG AACTACTGGA GACCACGTGC CGCCTGGCCA ACACGCTGAA GAGGCATGGA 
GTCCACCGTG GGGACCGTGT TGCCATCTAC ATGCCCGTGT CCCCATTGGC TGTGGCAGCA 
ATGCTGGCCT GTGCCAGGAT CGGAGCTGTC CACACAGTCA TCTTTGCTGG CTTCAGTGCA 
GAGTCCTTGG CTGGGAGGAT CAATGATGCC AAGTGCAAGG TGGTTATCAC CTTCAACCAA 
GGACTCCGGG GTGGGCGCGT GGTGGAGCTG AAGAAAATAG TGGATGAGGC TGTGAAGCAC 
TGCCCCACCG TGCAGCATGT CCTGGTGGCT CACAGGACAG ACAACAAGGT CCACATGGGG 
GATCTGGACG TCCCGCTGGA GCAGGAAATG GCCAAGGAGG ACCCTGTTTG CGCCCCAGAG 
AGCATGGGCA GTGAGGACAT GCTCTTCATG CTGTACACCT CAGGGAGCAC CGGAATGCCC 
AAGGGCATCG TCCATACCCA GGCAGGCTAC CTGCTCTATG CCGCCCTGAC TCACAAGCTT 
GTGTTTGACC ACCAGCCAGG TGACATCTTT GGCTGTGTGG CCGACATCGG TTGGATTACA 
GGACACAGCT ACGTGGTGTA TGGGCCTCTC TGCAATGGTG CCACCAGCGT CCTTTTTGAG 
AGCACCCCAG TTTATCCCAA TGCTGGTCGG TACTGGGAGA CAGTAGAGAG GTTGAAGATC 
AATCAGTTCT ATGGCGCCCC AACGGCTGTC CGGCTGTTGC TGAAATACGG TGATGCCTGG 
GTGAAGAAGT ATGATCGCTC CTCCCTGCGG ACCCTGGGGT CAGTGGGAGA GCCCATCAAC 
TGTGAGGCCT GGGAGTGGCT TCACAGGGTG GTGGGGGACA GCAGGTGCAC GCTGGTGGAC 
ACCTGGTGGC AGACAGAAAC AGGTGGCATC TGCATCGCAC CACGGCCCTC GGAAGAAGGG 
GCGGAAATCC TCCCTGCCAT GGCGATGAGG CCCTTCTTTG GCATCGTCCC CGTCCTCATG 
GATGAGAAGG GCAGCGTCGT GGAGGGCAGC AACGTCTCCG GGGCCCTGTG CATCTCCCAG 
GCCTGGCCGG GCATGGCCAG GACCATCTAT GGCGACCACC AGCGATTTGT GGACGCCTAC 
TTCAAGGCCT ACCCAGGCTA TTACTTCACT GGAGACGGGG CTTACCGAAC TGAGGGCGGC 
TATTACCAGA TCACAGGGCG GATGGATGAT GTCATCAACA TCAGTGGCCA CCGGCTGGGG 
ACCGCAGAGA TTGAGGACGC CATCGCCGAC CACCCTGCAG TACCAGAAAG TGCTGTCATT 
GGCTACCCCC ACGACATCAA AGGAGAAGCT GCCTTTGCCT TCATTGTGGT GAAAGATAGT 
GCGGGTGACT CAGATGTGGT GGTGCAGGAG CTCAAGTCCA TGGTGGCCAC CAAGATCGCC 
AAATATGCTG TGCCTGATGA GATCCTGGTG GTGAAACGTC TTCCAAAAAC CAGGTCTGGG 
AAGGTCATGC GGCGGCTCCT GAGGAAGATC ATCACTAGTG AGGCCCAGGA GCTGGGAGAC 
ACTACCACCT TGGAGGACCC CAGCATCATC GCAGAGATCC TGAGTGTCTA CCAGAAGTGC 
AAGGACAAGC AGGCTGCTGC TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239605.1
CDS	271..1977
Translation	MRRRKERQPWDRFHFLHFAPHGRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKHCPTVQHVLVAHRTDNKVHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK

Target ORF information:

RefSeq Version	NM_001252676.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252676.1

ORF Insert Sequence:

ATGAGGCGCC GAAAGGAAAG ACAACCTTGG GATCGCTTCC ACTTCCTCCA CTTCGCACCG 
CATGGCCGGG AACTACTGGA GACCACGTGC CGCCTGGCCA ACACGCTGAA GAGGCATGGA 
GTCCACCGTG GGGACCGTGT TGCCATCTAC ATGCCCGTGT CCCCATTGGC TGTGGCAGCA 
ATGCTGGCCT GTGCCAGGAT CGGAGCTGTC CACACAGTCA TCTTTGCTGG CTTCAGTGCA 
GAGTCCTTGG CTGGGAGGAT CAATGATGCC AAGTGCAAGG TGGTTATCAC CTTCAACCAA 
GGACTCCGGG GTGGGCGCGT GGTGGAGCTG AAGAAAATAG TGGATGAGGC TGTGAAGCAC 
TGCCCCACCG TGCAGCATGT CCTGGTGGCT CACAGGACAG ACAACAAGGT CCACATGGGG 
GATCTGGACG TCCCGCTGGA GCAGGAAATG GCCAAGGAGG ACCCTGTTTG CGCCCCAGAG 
AGCATGGGCA GTGAGGACAT GCTCTTCATG CTGTACACCT CAGGGAGCAC CGGAATGCCC 
AAGGGCATCG TCCATACCCA GGCAGGCTAC CTGCTCTATG CCGCCCTGAC TCACAAGCTT 
GTGTTTGACC ACCAGCCAGG TGACATCTTT GGCTGTGTGG CCGACATCGG TTGGATTACA 
GGACACAGCT ACGTGGTGTA TGGGCCTCTC TGCAATGGTG CCACCAGCGT CCTTTTTGAG 
AGCACCCCAG TTTATCCCAA TGCTGGTCGG TACTGGGAGA CAGTAGAGAG GTTGAAGATC 
AATCAGTTCT ATGGCGCCCC AACGGCTGTC CGGCTGTTGC TGAAATACGG TGATGCCTGG 
GTGAAGAAGT ATGATCGCTC CTCCCTGCGG ACCCTGGGGT CAGTGGGAGA GCCCATCAAC 
TGTGAGGCCT GGGAGTGGCT TCACAGGGTG GTGGGGGACA GCAGGTGCAC GCTGGTGGAC 
ACCTGGTGGC AGACAGAAAC AGGTGGCATC TGCATCGCAC CACGGCCCTC GGAAGAAGGG 
GCGGAAATCC TCCCTGCCAT GGCGATGAGG CCCTTCTTTG GCATCGTCCC CGTCCTCATG 
GATGAGAAGG GCAGCGTCGT GGAGGGCAGC AACGTCTCCG GGGCCCTGTG CATCTCCCAG 
GCCTGGCCGG GCATGGCCAG GACCATCTAT GGCGACCACC AGCGATTTGT GGACGCCTAC 
TTCAAGGCCT ACCCAGGCTA TTACTTCACT GGAGACGGGG CTTACCGAAC TGAGGGCGGC 
TATTACCAGA TCACAGGGCG GATGGATGAT GTCATCAACA TCAGTGGCCA CCGGCTGGGG 
ACCGCAGAGA TTGAGGACGC CATCGCCGAC CACCCTGCAG TACCAGAAAG TGCTGTCATT 
GGCTACCCCC ACGACATCAA AGGAGAAGCT GCCTTTGCCT TCATTGTGGT GAAAGATAGT 
GCGGGTGACT CAGATGTGGT GGTGCAGGAG CTCAAGTCCA TGGTGGCCAC CAAGATCGCC 
AAATATGCTG TGCCTGATGA GATCCTGGTG GTGAAACGTC TTCCAAAAAC CAGGTCTGGG 
AAGGTCATGC GGCGGCTCCT GAGGAAGATC ATCACTAGTG AGGCCCAGGA GCTGGGAGAC 
ACTACCACCT TGGAGGACCC CAGCATCATC GCAGAGATCC TGAGTGTCTA CCAGAAGTGC 
AAGGACAAGC AGGCTGCTGC TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSS1 ( XM_011529388.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527690.1 Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant X3, mRNA.

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CloneID	OHu73567
Clone ID Related Accession (Same CDS sequence)	XM_011529388.1
Accession Version	XM_011529388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCCA CCGTGGGGAC CGTGTTGCCA TCTACATGCC CGTGTCCCCA TTGGCTGTGG 
CAGCAATGCT GGCCTGTGCC AGGATCGGAG CTGTCCACAC AGTCATCTTT GCTGGCTTCA 
GTGCAGAGTC CTTGGCTGGG AGGATCAATG ATGGAAATGG CCAAGGAGGA CCCTGTTTGC 
GCCCCAGAGA GCATGGGCAG TGAGGACATG CTCTTCATGC TGTACACCTC AGGGAGCACC 
GGAATGCCCA AGGGCATCGT CCATACCCAG GCAGGCTACC TGCTCTATGC CGCCCTGACT 
CACAAGCTTG TGTTTGACCA CCAGCCAGGT GACATCTTTG GCTGTGTGGC CGACATCGGT 
TGGATTACAG GACACAGCTA CGTGGTGTAT GGGCCTCTCT GCAATGGTGC CACCAGCGTC 
CTTTTTGAGA GCACCCCAGT TTATCCCAAT GCTGGTCGGT ACTGGGAGAC AGTAGAGAGG 
TTGAAGATCA ATCAGTTCTA TGGCGCCCCA ACGGCTGTCC GGCTGTTGCT GAAATACGGT 
GATGCCTGGG TGAAGAAGTA TGATCGCTCC TCCCTGCGGA CCCTGGGGTC AGTGGGAGAG 
CCCATCAACT GTGAGGCCTG GGAGTGGCTT CACAGGGTGG TGGGGGACAG CAGGTGCACG 
CTGGTGGACA CCTGGTGGCA GACAGAAACA GGTGGCATCT GCATCGCACC ACGGCCCTCG 
GAAGAAGGGG CGGAAATCCT CCCTGCCATG GCGATGAGGC CCTTCTTTGG CATCGTCCCC 
GTCCTCATGG ATGAGAAGGG CAGCGTCGTG GAGGGCAGCA ACGTCTCCGG GGCCCTGTGC 
ATCTCCCAGG CCTGGCCGGG CATGGCCAGG ACCATCTATG GCGACCACCA GCGATTTGTG 
GACGCCTACT TCAAGGCCTA CCCAGGCTAT TACTTCACTG GAGACGGGGC TTACCGAACT 
GAGGGCGGCT ATTACCAGAT CACAGGGCGG ATGGATGATG TCATCAACAT CAGTGGCCAC 
CGGCTGGGGA CCGCAGAGAT TGAGGACGCC ATCGCCGACC ACCCTGCAGT ACCAGAAAGT 
GCTGTCATTG GCTACCCCCA CGACATCAAA GGAGAAGCTG CCTTTGCCTT CATTGTGGTG 
AAAGATAGTG CGGGTGACTC AGATGTGGTG GTGCAGGAGC TCAAGTCCAT GGTGGCCACC 
AAGATCGCCA AATATGCTGT GCCTGATGAG ATCCTGGTGG TGAAACGTCT TCCAAAAACC 
AGGTCTGGGA AGGTCATGCG GCGGCTCCTG AGGAAGATCA TCACTAGTGA GGCCCAGGAG 
CTGGGAGACA CTACCACCTT GGAGGACCCC AGCATCATCG CAGAGATCCT GAGTGTCTAC 
CAGAAGTGCA AGGACAAGCA GGCTGCTGCT AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527690.1
CDS	520..1935
Translation	MESTVGTVLPSTCPCPHWLWQQCWPVPGSELSTQSSLLASVQSPWLGGSMMEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK

Target ORF information:

RefSeq Version	XM_011529388.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529388.1

ORF Insert Sequence:

ATGGAGTCCA CCGTGGGGAC CGTGTTGCCA TCTACATGCC CGTGTCCCCA TTGGCTGTGG 
CAGCAATGCT GGCCTGTGCC AGGATCGGAG CTGTCCACAC AGTCATCTTT GCTGGCTTCA 
GTGCAGAGTC CTTGGCTGGG AGGATCAATG ATGGAAATGG CCAAGGAGGA CCCTGTTTGC 
GCCCCAGAGA GCATGGGCAG TGAGGACATG CTCTTCATGC TGTACACCTC AGGGAGCACC 
GGAATGCCCA AGGGCATCGT CCATACCCAG GCAGGCTACC TGCTCTATGC CGCCCTGACT 
CACAAGCTTG TGTTTGACCA CCAGCCAGGT GACATCTTTG GCTGTGTGGC CGACATCGGT 
TGGATTACAG GACACAGCTA CGTGGTGTAT GGGCCTCTCT GCAATGGTGC CACCAGCGTC 
CTTTTTGAGA GCACCCCAGT TTATCCCAAT GCTGGTCGGT ACTGGGAGAC AGTAGAGAGG 
TTGAAGATCA ATCAGTTCTA TGGCGCCCCA ACGGCTGTCC GGCTGTTGCT GAAATACGGT 
GATGCCTGGG TGAAGAAGTA TGATCGCTCC TCCCTGCGGA CCCTGGGGTC AGTGGGAGAG 
CCCATCAACT GTGAGGCCTG GGAGTGGCTT CACAGGGTGG TGGGGGACAG CAGGTGCACG 
CTGGTGGACA CCTGGTGGCA GACAGAAACA GGTGGCATCT GCATCGCACC ACGGCCCTCG 
GAAGAAGGGG CGGAAATCCT CCCTGCCATG GCGATGAGGC CCTTCTTTGG CATCGTCCCC 
GTCCTCATGG ATGAGAAGGG CAGCGTCGTG GAGGGCAGCA ACGTCTCCGG GGCCCTGTGC 
ATCTCCCAGG CCTGGCCGGG CATGGCCAGG ACCATCTATG GCGACCACCA GCGATTTGTG 
GACGCCTACT TCAAGGCCTA CCCAGGCTAT TACTTCACTG GAGACGGGGC TTACCGAACT 
GAGGGCGGCT ATTACCAGAT CACAGGGCGG ATGGATGATG TCATCAACAT CAGTGGCCAC 
CGGCTGGGGA CCGCAGAGAT TGAGGACGCC ATCGCCGACC ACCCTGCAGT ACCAGAAAGT 
GCTGTCATTG GCTACCCCCA CGACATCAAA GGAGAAGCTG CCTTTGCCTT CATTGTGGTG 
AAAGATAGTG CGGGTGACTC AGATGTGGTG GTGCAGGAGC TCAAGTCCAT GGTGGCCACC 
AAGATCGCCA AATATGCTGT GCCTGATGAG ATCCTGGTGG TGAAACGTCT TCCAAAAACC 
AGGTCTGGGA AGGTCATGCG GCGGCTCCTG AGGAAGATCA TCACTAGTGA GGCCCAGGAG 
CTGGGAGACA CTACCACCTT GGAGGACCCC AGCATCATCG CAGAGATCCT GAGTGTCTAC 
CAGAAGTGCA AGGACAAGCA GGCTGCTGCT AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSS1 ( NM_001252675.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001239604.1 Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 2, mRNA.

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CloneID	OHu09617
Clone ID Related Accession (Same CDS sequence)	NM_001252675.1
Accession Version	NM_001252675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM150326.1, BC044588.1, AK092295.1, AK125923.1 and AW024920.1. Transcript Variant: This variant (2) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC044588.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC GCACCCTGGG CCGCGGCGTC GGGAGGCTGC TGGGCAGCCT GCGAGGGCTC 
TCGGGGCAGC CCGCGCGGCC GCCGTGCGGG GTGAGCGCGC CGCGCAGGGC GGCCTCGGGA 
CCCTCGGGCA GCGCTCCCGC AGTTGCAGCA GCAGCAGCAC AGCCAGGCTC GTATCCCGCG 
CTGAGTGCAC AGGCAGCCCG GGAGCCGGCC GCCTTCTGGG GGCCTCTGGC GCGGGACACT 
CTCGTGTGGG ACACCCCCTA CCACACCGTC TGGGACTGCG ACTTCAGCAC TGGCAAGATC 
GGCTGGTTCC TGGGAGGCCA GTTAAATGTC TCTGTCAACT GCTTGGACCA GCATGTTCGG 
AAGTCCCCCG AGAGCGTTGC TTTGATCTGG GAGCGCGATG AGCCTGGAAC GGAAGTGAGG 
ATCACCTACA GGGAACTACT GGAGACCACG TGCCGCCTGG CCAACACGCT GAAGAGGCAT 
GGAGTCCACC GTGGGGACCG TGTTGCCATC TACATGCCCG TGTCCCCATT GGCTGTGGCA 
GCAATGCTGG CCTGTGCCAG GATCGGAGCT GTCCACACAG TCATCTTTGC TGGCTTCAGT 
GCAGAGTCCT TGGCTGGGAG GATCAATGAT GCCAAGTGCA AGGTGGTTAT CACCTTCAAC 
CAAGGACTCC GGGGTGGGCG CGTGGTGGAG CTGAAGAAAA TAGTGGATGA GGCTGTGAAG 
CACTGCCCCA CCGTGCAGCA TGTCCTGGTG GCTCACAGGA CAGACAACAA GGTCCACATG 
GGGGATCTGG ACGTCCCGCT GGAGCAGGAA ATGGCCAAGG AGGACCCTGT TTGCGCCCCA 
GAGAGCATGG GCAGTGAGGA CATGCTCTTC ATGCTGTACA CCTCAGGGAG CACCGGAATG 
CCCAAGGGCA TCGTCCATAC CCAGGCAGGC TACCTGCTCT ATGCCGCCCT GACTCACAAG 
CTTGTGTTTG ACCACCAGCC AGGTGACATC TTTGGCTGTG TGGCCGACAT CGGTTGGATT 
ACAGGACACA GCTACGTGGT GTATGGGCCT CTCTGCAATG GTGCCACCAG CGTCCTTTTT 
GAGAGCACCC CAGTTTATCC CAATGCTGGT CGGTACTGGG AGACAGTAGA GAGGTTGAAG 
ATCAATCAGT TCTATGGCGC CCCAACGGCT GTCCGGCTGT TGCTGAAATA CGGTGATGCC 
TGGGTGAAGA AGTATGATCG CTCCTCCCTG CGGACCCTGG GGTCAGTGGG AGAGCCCATC 
AACTGTGAGG CCTGGGAGTG GCTTCACAGG GTGGTGGGGG ACAGCAGGTG CACGCTGGTG 
GACACCTGGT GGCAGACAGG TGGCATCTGC ATCGCACCAC GGCCCTCGGA AGAAGGGGCG 
GAAATCCTCC CTGCCATGGC GATGAGGCCC TTCTTTGGCA TCGTCCCCGT CCTCATGGAT 
GAGAAGGGCA GCGTCGTGGA GGGCAGCAAC GTCTCCGGGG CCCTGTGCAT CTCCCAGGCC 
TGGCCGGGCA TGGCCAGGAC CATCTATGGC GACCACCAGC GATTTGTGGA CGCCTACTTC 
AAGGCCTACC CAGGCTATTA CTTCACTGGA GACGGGGCTT ACCGAACTGA GGGCGGCTAT 
TACCAGATCA CAGGGCGGAT GGATGATGTC ATCAACATCA GTGGCCACCG GCTGGGGACC 
GCAGAGATTG AGGACGCCAT CGCCGACCAC CCTGCAGTAC CAGAAAGTGC TGTCATTGGC 
TACCCCCACG ACATCAAAGG AGAAGCTGCC TTTGCCTTCA TTGTGGTGAA AGATAGTGCG 
GGTGACTCAG ATGTGGTGGT GCAGGAGCTC AAGTCCATGG TGGCCACCAA GATCGCCAAA 
TATGCTGTGC CTGATGAGAT CCTGGTGGTG AAACGTCTTC CAAAAACCAG GTCTGGGAAG 
GTCATGCGGC GGCTCCTGAG GAAGATCATC ACTAGTGAGG CCCAGGAGCT GGGAGACACT 
ACCACCTTGG AGGACCCCAG CATCATCGCA GAGATCCTGA GTGTCTACCA GAAGTGCAAG 
GACAAGCAGG CTGCTGCTAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239604.1
CDS	81..2144
Translation	MAARTLGRGVGRLLGSLRGLSGQPARPPCGVSAPRRAASGPSGSAPAVAAAAAQPGSYPALSAQAAREPAAFWGPLARDTLVWDTPYHTVWDCDFSTGKIGWFLGGQLNVSVNCLDQHVRKSPESVALIWERDEPGTEVRITYRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKHCPTVQHVLVAHRTDNKVHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK

Target ORF information:

RefSeq Version	NM_001252675.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252675.1

ORF Insert Sequence:

ATGGCGGCGC GCACCCTGGG CCGCGGCGTC GGGAGGCTGC TGGGCAGCCT GCGAGGGCTC 
TCGGGGCAGC CCGCGCGGCC GCCGTGCGGG GTGAGCGCGC CGCGCAGGGC GGCCTCGGGA 
CCCTCGGGCA GCGCTCCCGC AGTTGCAGCA GCAGCAGCAC AGCCAGGCTC GTATCCCGCG 
CTGAGTGCAC AGGCAGCCCG GGAGCCGGCC GCCTTCTGGG GGCCTCTGGC GCGGGACACT 
CTCGTGTGGG ACACCCCCTA CCACACCGTC TGGGACTGCG ACTTCAGCAC TGGCAAGATC 
GGCTGGTTCC TGGGAGGCCA GTTAAATGTC TCTGTCAACT GCTTGGACCA GCATGTTCGG 
AAGTCCCCCG AGAGCGTTGC TTTGATCTGG GAGCGCGATG AGCCTGGAAC GGAAGTGAGG 
ATCACCTACA GGGAACTACT GGAGACCACG TGCCGCCTGG CCAACACGCT GAAGAGGCAT 
GGAGTCCACC GTGGGGACCG TGTTGCCATC TACATGCCCG TGTCCCCATT GGCTGTGGCA 
GCAATGCTGG CCTGTGCCAG GATCGGAGCT GTCCACACAG TCATCTTTGC TGGCTTCAGT 
GCAGAGTCCT TGGCTGGGAG GATCAATGAT GCCAAGTGCA AGGTGGTTAT CACCTTCAAC 
CAAGGACTCC GGGGTGGGCG CGTGGTGGAG CTGAAGAAAA TAGTGGATGA GGCTGTGAAG 
CACTGCCCCA CCGTGCAGCA TGTCCTGGTG GCTCACAGGA CAGACAACAA GGTCCACATG 
GGGGATCTGG ACGTCCCGCT GGAGCAGGAA ATGGCCAAGG AGGACCCTGT TTGCGCCCCA 
GAGAGCATGG GCAGTGAGGA CATGCTCTTC ATGCTGTACA CCTCAGGGAG CACCGGAATG 
CCCAAGGGCA TCGTCCATAC CCAGGCAGGC TACCTGCTCT ATGCCGCCCT GACTCACAAG 
CTTGTGTTTG ACCACCAGCC AGGTGACATC TTTGGCTGTG TGGCCGACAT CGGTTGGATT 
ACAGGACACA GCTACGTGGT GTATGGGCCT CTCTGCAATG GTGCCACCAG CGTCCTTTTT 
GAGAGCACCC CAGTTTATCC CAATGCTGGT CGGTACTGGG AGACAGTAGA GAGGTTGAAG 
ATCAATCAGT TCTATGGCGC CCCAACGGCT GTCCGGCTGT TGCTGAAATA CGGTGATGCC 
TGGGTGAAGA AGTATGATCG CTCCTCCCTG CGGACCCTGG GGTCAGTGGG AGAGCCCATC 
AACTGTGAGG CCTGGGAGTG GCTTCACAGG GTGGTGGGGG ACAGCAGGTG CACGCTGGTG 
GACACCTGGT GGCAGACAGG TGGCATCTGC ATCGCACCAC GGCCCTCGGA AGAAGGGGCG 
GAAATCCTCC CTGCCATGGC GATGAGGCCC TTCTTTGGCA TCGTCCCCGT CCTCATGGAT 
GAGAAGGGCA GCGTCGTGGA GGGCAGCAAC GTCTCCGGGG CCCTGTGCAT CTCCCAGGCC 
TGGCCGGGCA TGGCCAGGAC CATCTATGGC GACCACCAGC GATTTGTGGA CGCCTACTTC 
AAGGCCTACC CAGGCTATTA CTTCACTGGA GACGGGGCTT ACCGAACTGA GGGCGGCTAT 
TACCAGATCA CAGGGCGGAT GGATGATGTC ATCAACATCA GTGGCCACCG GCTGGGGACC 
GCAGAGATTG AGGACGCCAT CGCCGACCAC CCTGCAGTAC CAGAAAGTGC TGTCATTGGC 
TACCCCCACG ACATCAAAGG AGAAGCTGCC TTTGCCTTCA TTGTGGTGAA AGATAGTGCG 
GGTGACTCAG ATGTGGTGGT GCAGGAGCTC AAGTCCATGG TGGCCACCAA GATCGCCAAA 
TATGCTGTGC CTGATGAGAT CCTGGTGGTG AAACGTCTTC CAAAAACCAG GTCTGGGAAG 
GTCATGCGGC GGCTCCTGAG GAAGATCATC ACTAGTGAGG CCCAGGAGCT GGGAGACACT 
ACCACCTTGG AGGACCCCAG CATCATCGCA GAGATCCTGA GTGTCTACCA GAAGTGCAAG 
GACAAGCAGG CTGCTGCTAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSS1 ( NM_001252677.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001239606.1 Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 4, mRNA.

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CloneID	OHu55918
Clone ID Related Accession (Same CDS sequence)	NM_001252677.1
Accession Version	NM_001252677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM150326.1, AK127566.1, BC114612.1, AK125923.1 and AW024920.1. Transcript Variant: This variant (4) uses an alternate splice site in the 3' coding region, which results in a translational frameshift, compared to variant 1. The encoded isoform (4) is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK127566.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC GCACCCTGGG CCGCGGCGTC GGGAGGCTGC TGGGCAGCCT GCGAGGGCTC 
TCGGGGCAGC CCGCGCGGCC GCCGTGCGGG GTGAGCGCGC CGCGCAGGGC GGCCTCGGGA 
CCCTCGGGCA GCGCTCCCGC AGTTGCAGCA GCAGCAGCAC AGCCAGGCTC GTATCCCGCG 
CTGAGTGCAC AGGCAGCCCG GGAGCCGGCC GCCTTCTGGG GGCCTCTGGC GCGGGACACT 
CTCGTGTGGG ACACCCCCTA CCACACCGTC TGGGACTGCG ACTTCAGCAC TGGCAAGATC 
GGCTGGTTCC TGGGAGGCCA GTTAAATGTC TCTGTCAACT GCTTGGACCA GCATGTTCGG 
AAGTCCCCCG AGAGCGTTGC TTTGATCTGG GAGCGCGATG AGCCTGGAAC GGAAGTGAGG 
ATCACCTACA GGGAACTACT GGAGACCACG TGCCGCCTGG CCAACACGCT GAAGAGGCAT 
GGAGTCCACC GTGGGGACCG TGTTGCCATC TACATGCCCG TGTCCCCATT GGCTGTGGCA 
GCAATGCTGG CCTGTGCCAG GATCGGAGCT GTCCACACAG TCATCTTTGC TGGCTTCAGT 
GCAGAGTCCT TGGCTGGGAG GATCAATGAT GCCAAGTGCA AGGTGGTTAT CACCTTCAAC 
CAAGGACTCC GGGGTGGGCG CGTGGTGGAG CTGAAGAAAA TAGTGGATGA GGCTGTGAAG 
CACTGCCCCA CCGTGCAGCA TGTCCTGGTG GCTCACAGGA CAGACAACAA GGTCCACATG 
GGGGATCTGG ACGTCCCGCT GGAGCAGGAA ATGGCCAAGG AGGACCCTGT TTGCGCCCCA 
GAGAGCATGG GCAGTGAGGA CATGCTCTTC ATGCTGTACA CCTCAGGGAG CACCGGAATG 
CCCAAGGGCA TCGTCCATAC CCAGGCAGGC TACCTGCTCT ATGCCGCCCT GACTCACAAG 
CTTGTGTTTG ACCACCAGCC AGGTGACATC TTTGGCTGTG TGGCCGACAT CGGTTGGATT 
ACAGGACACA GCTACGTGGT GTATGGGCCT CTCTGCAATG GTGCCACCAG CGTCCTTTTT 
GAGAGCACCC CAGTTTATCC CAATGCTGGT CGGTACTGGG AGACAGTAGA GAGGTTGAAG 
ATCAATCAGT TCTATGGCGC CCCAACGGCT GTCCGGCTGT TGCTGAAATA CGGTGATGCC 
TGGGTGAAGA AGTATGATCG CTCCTCCCTG CGGACCCTGG GGTCAGTGGG AGAGCCCATC 
AACTGTGAGG CCTGGGAGTG GCTTCACAGG GTGGTGGGGG ACAGCAGGTG CACGCTGGTG 
GACACCTGGT GGCAGACAGA AACAGGTGGC ATCTGCATCG CACCACGGCC CTCGGAAGAA 
GGGGCGGAAA TCCTCCCTGC CATGGCGATG AGGCCCTTCT TTGGCATCGT CCCCGTCCTC 
ATGGATGAGA AGGGCAGCGT CGTGGAGGGC AGCAACGTCT CCGGGGCCCT GTGCATCTCC 
CAGGCCTGGC CGGGCATGGC CAGGACCATC TATGGCGACC ACCAGCGATT TGTGGACGCC 
TACTTCAAGG CCTACCCAGG CTATTACTTC ACTGGAGACG GGGCTTACCG AACTGAGGGC 
GGCTATTACC AGATCACAGG GCGGATGGAT GATGTCATCA ACATACTCCA AATAGTTGGG 
TTCTTCAGGG AAGCTATTAG AAACTCAGGT GACTTGTTAG AGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239606.1
CDS	81..1808
Translation	MAARTLGRGVGRLLGSLRGLSGQPARPPCGVSAPRRAASGPSGSAPAVAAAAAQPGSYPALSAQAAREPAAFWGPLARDTLVWDTPYHTVWDCDFSTGKIGWFLGGQLNVSVNCLDQHVRKSPESVALIWERDEPGTEVRITYRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKHCPTVQHVLVAHRTDNKVHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINILQIVGFFREAIRNSGDLLEH

Target ORF information:

RefSeq Version	NM_001252677.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252677.1

ORF Insert Sequence:

ATGGCGGCGC GCACCCTGGG CCGCGGCGTC GGGAGGCTGC TGGGCAGCCT GCGAGGGCTC 
TCGGGGCAGC CCGCGCGGCC GCCGTGCGGG GTGAGCGCGC CGCGCAGGGC GGCCTCGGGA 
CCCTCGGGCA GCGCTCCCGC AGTTGCAGCA GCAGCAGCAC AGCCAGGCTC GTATCCCGCG 
CTGAGTGCAC AGGCAGCCCG GGAGCCGGCC GCCTTCTGGG GGCCTCTGGC GCGGGACACT 
CTCGTGTGGG ACACCCCCTA CCACACCGTC TGGGACTGCG ACTTCAGCAC TGGCAAGATC 
GGCTGGTTCC TGGGAGGCCA GTTAAATGTC TCTGTCAACT GCTTGGACCA GCATGTTCGG 
AAGTCCCCCG AGAGCGTTGC TTTGATCTGG GAGCGCGATG AGCCTGGAAC GGAAGTGAGG 
ATCACCTACA GGGAACTACT GGAGACCACG TGCCGCCTGG CCAACACGCT GAAGAGGCAT 
GGAGTCCACC GTGGGGACCG TGTTGCCATC TACATGCCCG TGTCCCCATT GGCTGTGGCA 
GCAATGCTGG CCTGTGCCAG GATCGGAGCT GTCCACACAG TCATCTTTGC TGGCTTCAGT 
GCAGAGTCCT TGGCTGGGAG GATCAATGAT GCCAAGTGCA AGGTGGTTAT CACCTTCAAC 
CAAGGACTCC GGGGTGGGCG CGTGGTGGAG CTGAAGAAAA TAGTGGATGA GGCTGTGAAG 
CACTGCCCCA CCGTGCAGCA TGTCCTGGTG GCTCACAGGA CAGACAACAA GGTCCACATG 
GGGGATCTGG ACGTCCCGCT GGAGCAGGAA ATGGCCAAGG AGGACCCTGT TTGCGCCCCA 
GAGAGCATGG GCAGTGAGGA CATGCTCTTC ATGCTGTACA CCTCAGGGAG CACCGGAATG 
CCCAAGGGCA TCGTCCATAC CCAGGCAGGC TACCTGCTCT ATGCCGCCCT GACTCACAAG 
CTTGTGTTTG ACCACCAGCC AGGTGACATC TTTGGCTGTG TGGCCGACAT CGGTTGGATT 
ACAGGACACA GCTACGTGGT GTATGGGCCT CTCTGCAATG GTGCCACCAG CGTCCTTTTT 
GAGAGCACCC CAGTTTATCC CAATGCTGGT CGGTACTGGG AGACAGTAGA GAGGTTGAAG 
ATCAATCAGT TCTATGGCGC CCCAACGGCT GTCCGGCTGT TGCTGAAATA CGGTGATGCC 
TGGGTGAAGA AGTATGATCG CTCCTCCCTG CGGACCCTGG GGTCAGTGGG AGAGCCCATC 
AACTGTGAGG CCTGGGAGTG GCTTCACAGG GTGGTGGGGG ACAGCAGGTG CACGCTGGTG 
GACACCTGGT GGCAGACAGA AACAGGTGGC ATCTGCATCG CACCACGGCC CTCGGAAGAA 
GGGGCGGAAA TCCTCCCTGC CATGGCGATG AGGCCCTTCT TTGGCATCGT CCCCGTCCTC 
ATGGATGAGA AGGGCAGCGT CGTGGAGGGC AGCAACGTCT CCGGGGCCCT GTGCATCTCC 
CAGGCCTGGC CGGGCATGGC CAGGACCATC TATGGCGACC ACCAGCGATT TGTGGACGCC 
TACTTCAAGG CCTACCCAGG CTATTACTTC ACTGGAGACG GGGCTTACCG AACTGAGGGC 
GGCTATTACC AGATCACAGG GCGGATGGAT GATGTCATCA ACATACTCCA AATAGTTGGG 
TTCTTCAGGG AAGCTATTAG AAACTCAGGT GACTTGTTAG AGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSS1 ( XM_006723659.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723722.1 Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant X2, mRNA.

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CloneID	OHu52816
Clone ID Related Accession (Same CDS sequence)	XM_006723659.1
Accession Version	XM_006723659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC GCACCCTGGG CCGCGGCGTC GGGAGGCTGC TGGGCAGCCT GCGAGGGCTC 
TCGGGGCAGC CCGCGCGGCC GCCGTGCGGG GTGAGCGCGC CGCGCAGGGC GGCCTCGGGA 
CCCTCGGGCA GCGCTCCCGC AGTTGCAGCA GCAGCAGCAC AGCCAGGCTC GTATCCCGCG 
CTGAGTGCAC AGGCAGCCCG GGAGCCGGCC GCCTTCTGGG GGCCTCTGGC GCGGGACACT 
CTCGTGTGGG ACACCCCCTA CCACACCGTC TGGGACTGCG ACTTCAGCAC TGGCAAGATC 
GGCTGGTTCC TGGGAGGCCA GTTAAATGTC TCTGTCAACT GCTTGGACCA GCATGTTCGG 
AAGTCCCCCG AGAGCGTTGC TTTGATCTGG GAGCGCGATG AGCCTGGAAC GGAAGTGAGG 
ATCACCTACA GGGAACTACT GGAGACCACG TGCCGCCTGG CCAACACGCT GAAGAGGCAT 
GGAGTCCACC GTGGGGACCG TGTTGCCATC TACATGCCCG TGTCCCCATT GGCTGTGGCA 
GCAATGCTGG CCTGTGCCAG GATCGGAGCT GTCCACACAG TCATCTTTGC TGGCTTCAGT 
GCAGAGTCCT TGGCTGGGAG GATCAATGAT GTGGGAGAGC CCATCAACTG TGAGGCCTGG 
GAGTGGCTTC ACAGGGTGGT GGGGGACAGC AGGTGCACGC TGGTGGACAC CTGGTGGCAG 
ACAGAAACAG GTGGCATCTG CATCGCACCA CGGCCCTCGG AAGAAGGGGC GGAAATCCTC 
CCTGCCATGG CGATGAGGCC CTTCTTTGGC ATCGTCCCCG TCCTCATGGA TGAGAAGGGC 
AGCGTCGTGG AGGGCAGCAA CGTCTCCGGG GCCCTGTGCA TCTCCCAGGC CTGGCCGGGC 
ATGGCCAGGA CCATCTATGG CGACCACCAG CGATTTGTGG ACGCCTACTT CAAGGCCTAC 
CCAGGCTATT ACTTCACTGG AGACGGGGCT TACCGAACTG AGGGCGGCTA TTACCAGATC 
ACAGGGCGGA TGGATGATGT CATCAACATC AGTGGCCACC GGCTGGGGAC CGCAGAGATT 
GAGGACGCCA TCGCCGACCA CCCTGCAGTA CCAGAAAGTG CTGTCATTGG CTACCCCCAC 
GACATCAAAG GAGAAGCTGC CTTTGCCTTC ATTGTGGTGA AAGATAGTGC GGGTGACTCA 
GATGTGGTGG TGCAGGAGCT CAAGTCCATG GTGGCCACCA AGATCGCCAA ATATGCTGTG 
CCTGATGAGA TCCTGGTGGT GAAACGTCTT CCAAAAACCA GGTCTGGGAA GGTCATGCGG 
CGGCTCCTGA GGAAGATCAT CACTAGTGAG GCCCAGGAGC TGGGAGACAC TACCACCTTG 
GAGGACCCCA GCATCATCGC AGAGATCCTG AGTGTCTACC AGAAGTGCAA GGACAAGCAG 
GCTGCTGCTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723722.1
CDS	42..1496
Translation	MAARTLGRGVGRLLGSLRGLSGQPARPPCGVSAPRRAASGPSGSAPAVAAAAAQPGSYPALSAQAAREPAAFWGPLARDTLVWDTPYHTVWDCDFSTGKIGWFLGGQLNVSVNCLDQHVRKSPESVALIWERDEPGTEVRITYRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK

Target ORF information:

RefSeq Version	XM_006723659.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA synthetase short chain family member 1 (ACSS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723659.1

ORF Insert Sequence:

ATGGCGGCGC GCACCCTGGG CCGCGGCGTC GGGAGGCTGC TGGGCAGCCT GCGAGGGCTC 
TCGGGGCAGC CCGCGCGGCC GCCGTGCGGG GTGAGCGCGC CGCGCAGGGC GGCCTCGGGA 
CCCTCGGGCA GCGCTCCCGC AGTTGCAGCA GCAGCAGCAC AGCCAGGCTC GTATCCCGCG 
CTGAGTGCAC AGGCAGCCCG GGAGCCGGCC GCCTTCTGGG GGCCTCTGGC GCGGGACACT 
CTCGTGTGGG ACACCCCCTA CCACACCGTC TGGGACTGCG ACTTCAGCAC TGGCAAGATC 
GGCTGGTTCC TGGGAGGCCA GTTAAATGTC TCTGTCAACT GCTTGGACCA GCATGTTCGG 
AAGTCCCCCG AGAGCGTTGC TTTGATCTGG GAGCGCGATG AGCCTGGAAC GGAAGTGAGG 
ATCACCTACA GGGAACTACT GGAGACCACG TGCCGCCTGG CCAACACGCT GAAGAGGCAT 
GGAGTCCACC GTGGGGACCG TGTTGCCATC TACATGCCCG TGTCCCCATT GGCTGTGGCA 
GCAATGCTGG CCTGTGCCAG GATCGGAGCT GTCCACACAG TCATCTTTGC TGGCTTCAGT 
GCAGAGTCCT TGGCTGGGAG GATCAATGAT GTGGGAGAGC CCATCAACTG TGAGGCCTGG 
GAGTGGCTTC ACAGGGTGGT GGGGGACAGC AGGTGCACGC TGGTGGACAC CTGGTGGCAG 
ACAGAAACAG GTGGCATCTG CATCGCACCA CGGCCCTCGG AAGAAGGGGC GGAAATCCTC 
CCTGCCATGG CGATGAGGCC CTTCTTTGGC ATCGTCCCCG TCCTCATGGA TGAGAAGGGC 
AGCGTCGTGG AGGGCAGCAA CGTCTCCGGG GCCCTGTGCA TCTCCCAGGC CTGGCCGGGC 
ATGGCCAGGA CCATCTATGG CGACCACCAG CGATTTGTGG ACGCCTACTT CAAGGCCTAC 
CCAGGCTATT ACTTCACTGG AGACGGGGCT TACCGAACTG AGGGCGGCTA TTACCAGATC 
ACAGGGCGGA TGGATGATGT CATCAACATC AGTGGCCACC GGCTGGGGAC CGCAGAGATT 
GAGGACGCCA TCGCCGACCA CCCTGCAGTA CCAGAAAGTG CTGTCATTGG CTACCCCCAC 
GACATCAAAG GAGAAGCTGC CTTTGCCTTC ATTGTGGTGA AAGATAGTGC GGGTGACTCA 
GATGTGGTGG TGCAGGAGCT CAAGTCCATG GTGGCCACCA AGATCGCCAA ATATGCTGTG 
CCTGATGAGA TCCTGGTGGT GAAACGTCTT CCAAAAACCA GGTCTGGGAA GGTCATGCGG 
CGGCTCCTGA GGAAGATCAT CACTAGTGAG GCCCAGGAGC TGGGAGACAC TACCACCTTG 
GAGGACCCCA GCATCATCGC AGAGATCCTG AGTGTCTACC AGAAGTGCAA GGACAAGCAG 
GCTGCTGCTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glucose-independent Acetate Metabolism Promotes Melanoma Cell Survival and Tumor Growth.
The Journal of biological chemistry291(42)21869-21879(2016 Oct)
Lakhter AJ,Hamilton J,Konger RL,Brustovetsky N,Broxmeyer HE,Naidu SR

Gene network analysis reveals a novel 22-gene signature of carbon metabolism in hepatocellular carcinoma.
Oncotarget7(31)49232-49245(2016 Aug)
Zhang J,Baddoo M,Han C,Strong MJ,Cvitanovic J,Moroz K,Dash S,Flemington EK,Wu T

A novel Acetyl-CoA synthetase short-chain subfamily member 1 (Acss1) gene indicates a dynamic history of paralogue retention and loss in vertebrates.
Gene497(2)249-55(2012 Apr)
Castro LF,Lopes-Marques M,Wilson JM,Rocha E,Reis-Henriques MA,Santos MM,Cunha I

Common genetic variation and performance on standardized cognitive tests.
European journal of human genetics : EJHG18(7)815-20(2010 Jul)
Cirulli ET,Kasperavici?te D,Attix DK,Need AC,Ge D,Gibson G,Goldstein DB

The importance of acetyl coenzyme A synthetase for 11C-acetate uptake and cell survival in hepatocellular carcinoma.
Journal of nuclear medicine : official publication, Society of Nuclear Medicine50(8)1222-8(2009 Aug)
Yun M,Bang SH,Kim JW,Park JY,Kim KS,Lee JD

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ACSS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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