Acss1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acss1
Entrez Gene ID	296259
Full Name	acyl-CoA synthetase short-chain family member 1
Synonyms	Acas2l
General protein information	Preferred Names acyl-CoA synthetase short-chain family member 1 Names acetyl-coenzyme A synthetase 2-like, mitochondrial acetyl-Coenzyme A synthetase 2 (AMP forming)-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q41

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106524.1	NP_001099994.1	acetyl-coenzyme A synthetase 2-like, mitochondrial
XM_008762307.2	XP_008760529.1	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	acss1	NP_001074125.3
Homo sapiens (human)	ACSS1	NP_115890.2
Pan troglodytes (chimpanzee)	ACSS1	XP_001149851.1
	ACSS1	XP_001099300.2
Canis lupus familiaris (dog)	ACSS1	XP_534200.4
Bos taurus (cattle)	ACSS1	NP_777171.1
Mus musculus (house mouse)	Acss1	NP_542142.1
Gallus gallus (chicken)	ACSS1	XP_415011.4
Saccharomyces cerevisiae (baker's yeast)	ACS2	NP_013254.1
Neurospora crassa	NCU06836	XP_963677.2
Xenopus tropicalis (tropical clawed frog)	acss1	XP_002937499.1
Eremothecium gossypii	AGOS_AGL148C	NP_986519.1
Rattus norvegicus (Norway rat)	Acss1	NP_001099994.1
Kluyveromyces lactis	KLLA0D17336g	XP_453827.1
Schizosaccharomyces pombe (fission yeast)	SPCC191.02c	NP_588291.2
Magnaporthe oryzae (rice blast fungus)	MGG_03201	XP_003716783.1

Gene Ontology
Bibliography

Related articles in PubMed

Glucose-independent Acetate Metabolism Promotes Melanoma Cell Survival and Tumor Growth.
Lakhter AJ, Hamilton J, Konger RL, Brustovetsky N, Broxmeyer HE, Naidu SR
The Journal of biological chemistry291(42)21869-21879(2016 Oct)

Fasting-induced hypothermia and reduced energy production in mice lacking acetyl-CoA synthetase 2.
Sakakibara I, Fujino T, Ishii M, Tanaka T, Shimosawa T, Miura S, Zhang W, Tokutake Y, Yamamoto J, Awano M, Iwasaki S, Motoike T, Okamura M, Inagaki T, Kita K, Ezaki O, Naito M, Kuwaki T, Chohnan S, Yamamoto TT, Hammer RE, Kodama T, Yanagisawa M, Sakai J
Cell metabolism9(2)191-202(2009 Feb)

Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome.
Watkins PA, Maiguel D, Jia Z, Pevsner J
Journal of lipid research48(12)2736-50(2007 Dec)

Reversible lysine acetylation controls the activity of the mitochondrial enzyme acetyl-CoA synthetase 2.
Schwer B, Bunkenborg J, Verdin RO, Andersen JS, Verdin E
Proceedings of the National Academy of Sciences of the United States of America103(27)10224-9(2006 Jul)

Acetyl-CoA synthetase 2, a mitochondrial matrix enzyme involved in the oxidation of acetate.
Fujino T, Kondo J, Ishikawa M, Morikawa K, Yamamoto TT
The Journal of biological chemistry276(14)11420-6(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Acss1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acss1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa45363	XM_008762307.2 Latest version!	Rattus norvegicus acyl-CoA synthetase short-chain family member 1 (Acss1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa01545	NM_001106524.1 Latest version!	Rattus norvegicus acyl-CoA synthetase short-chain family member 1 (Acss1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acss1 ( XM_008762307.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760529.1 Rattus norvegicus acyl-CoA synthetase short-chain family member 1 (Acss1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa45363
Clone ID Related Accession (Same CDS sequence)	XM_008762307.2
Accession Version	XM_008762307.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	acetyl-coenzyme A synthetase 2-like, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762307.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTTCC CCACCCCACC CCCTACCCCT GCCACCCTGA GAGACTCATC TGCTGACTGT 
GTGCAGCGTG TGGTGCTAAA AGGTAGCAGA CCCTTCAAGA GGGAGCTTCT GGAGACCACA 
TGCCGCCTGG CCAACACGCT GAAGAGGCAT GGTGTGCACC GAGGGGACCG TGTGGCCATC 
TACATGCCTG TGTCCCCACT GGCTGTGGCA GCGATGCTGG CCTGTGCCAG GATTGGAGCT 
ATCCACACAG TGGTTTTTGC TGGCTTTAGT GCAGAGTCCT TGGCCGGGAG GATCAATGAT 
GCCAAGTGCA AGGTTGTTAT CACCTTCAAC CAAGGACTCC GGGGAGGGCG TGTGGTGGAG 
CTGAAGAAAA TAGTGGACGA AGCTGTGAAG AACTGCCCAA CAGTGCAGCA TGTCCTAGTG 
GCTCACAGGA CGGACACCAA GGTTCCCATG GGGAATCTGG ACATCCCCCT TGAGCAGGAG 
ATGGCCAATG AGGCCCCTGT GTGTACCCCT GAAAGCATGA ACAGTGAAGA TATGCTCTTC 
ATGCTCTACA CGTCTGGGAG CACTGGGACA CCCAAGGGAC TCGTTCATAC ACAGGCAGGC 
TATCTGCTGT ATGCCGCCAT GACGCACAAG CTCGTGTTTG ACTACCATCC AGGTGATGTC 
TTTGGTTGTG TGGCTGACAT CGGTTGGATC ACAGGACACA GCTATGTGGT GTATGGACCC 
CTCTGCAATG GAGCTACCAC GGTCCTTTTT GAGAGCACCC CAGTTTACCC TGATGCTGGT 
CGTTACTGGG AGACAGTACA GAGGCTAAAG ATCAACCAGT TCTATGGTGC CCCGACAGCT 
GTCCGACTGC TGCTGAAGTT CGGGGATGCC TGGGTGAAAA AGTATGACCG CTCCTCCCTG 
CGCACACTGG GGTCAGTGGG AGAGCCCATC AATCATGAGG CCTGGGAGTG GCTCCACAAA 
GTGGTGGGTG ATGGCAGATG TACCCTGGTG GACACTTGGT GGCAGACAGA AACTGGAGGC 
ATCTGCATTG CACCACGGCC CTCGGAAGAT GGGGCAGAGA TCCTCCCAGG CATGGCCATG 
AGGCCGTTTT TTGGCATTGT CCCTGCACTC ATGGATGAGA AGGGCAATGT TGTGGAGGGT 
GGTGATGTCT CTGGGGCCCT GTGTATTTCC CAAGCTTGGC CAGGCATGGC AAGGACCATC 
TACGGTGACC ACCAGAGGTT CGTAGATGCC TACTTCAGAA CGTACCCAGG TTACTACTTC 
ACTGGAGATG GAGCTCACCG GACGGAGGGT GGCTATTACC AGATCACAGG GCGCATGGAT 
GATGTCATCA ACATCAGTGG TCATCGCCTG GGGACTGCAG AGATTGAGGA TGCAATGGCT 
GACCATCCCG CTGTCCCAGA AACTGCTGTC ATTGGGTACC CTCATGACAT CAAAGGAGAA 
GCTGCGTTTG CCTTCATTGT ACTGAAAGAT GACGTCCGTG ATGCAAACGT GGTGGTAAAT 
GAGCTCAAGT TGGCAGTGGC CACCAAGATC GCCAAGTATG CTGTGCCTGA CCAGATCCTG 
GTGGTGAAGC GTCTCCCCAA AACCAGGTCT GGGAAGGTGA TGAGGAGGCT GCTGAGGAAG 
ATCATCACCA GCAGGGGGCA GGATCTAGGG GACACCACTA CCCTGGAGGA CCCCAGTGTC 
ATCACGGAGA TCATGAGTGC CTTTCGGGAG TACGAAGAGC AGCGGGCTGC CACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760529.1
CDS	1231..2970
Translation	MLFPTPPPTPATLRDSSADCVQRVVLKGSRPFKRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAIHTVVFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKNCPTVQHVLVAHRTDTKVPMGNLDIPLEQEMANEAPVCTPESMNSEDMLFMLYTSGSTGTPKGLVHTQAGYLLYAAMTHKLVFDYHPGDVFGCVADIGWITGHSYVVYGPLCNGATTVLFESTPVYPDAGRYWETVQRLKINQFYGAPTAVRLLLKFGDAWVKKYDRSSLRTLGSVGEPINHEAWEWLHKVVGDGRCTLVDTWWQTETGGICIAPRPSEDGAEILPGMAMRPFFGIVPALMDEKGNVVEGGDVSGALCISQAWPGMARTIYGDHQRFVDAYFRTYPGYYFTGDGAHRTEGGYYQITGRMDDVINISGHRLGTAEIEDAMADHPAVPETAVIGYPHDIKGEAAFAFIVLKDDVRDANVVVNELKLAVATKIAKYAVPDQILVVKRLPKTRSGKVMRRLLRKIITSRGQDLGDTTTLEDPSVITEIMSAFREYEEQRAATN

Target ORF information:

RefSeq Version	XM_008762307.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase short-chain family member 1 (Acss1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762307.2

ORF Insert Sequence:

ATGTTGTTCC CCACCCCACC CCCTACCCCT GCCACCCTGA GAGACTCATC TGCTGACTGT 
GTGCAGCGTG TGGTGCTAAA AGGTAGCAGA CCCTTCAAGA GGGAGCTTCT GGAGACCACA 
TGCCGCCTGG CCAACACGCT GAAGAGGCAT GGTGTGCACC GAGGGGACCG TGTGGCCATC 
TACATGCCTG TGTCCCCACT GGCTGTGGCA GCGATGCTGG CCTGTGCCAG GATTGGAGCT 
ATCCACACAG TGGTTTTTGC TGGCTTTAGT GCAGAGTCCT TGGCCGGGAG GATCAATGAT 
GCCAAGTGCA AGGTTGTTAT CACCTTCAAC CAAGGACTCC GGGGAGGGCG TGTGGTGGAG 
CTGAAGAAAA TAGTGGACGA AGCTGTGAAG AACTGCCCAA CAGTGCAGCA TGTCCTAGTG 
GCTCACAGGA CGGACACCAA GGTTCCCATG GGGAATCTGG ACATCCCCCT TGAGCAGGAG 
ATGGCCAATG AGGCCCCTGT GTGTACCCCT GAAAGCATGA ACAGTGAAGA TATGCTCTTC 
ATGCTCTACA CGTCTGGGAG CACTGGGACA CCCAAGGGAC TCGTTCATAC ACAGGCAGGC 
TATCTGCTGT ATGCCGCCAT GACGCACAAG CTCGTGTTTG ACTACCATCC AGGTGATGTC 
TTTGGTTGTG TGGCTGACAT CGGTTGGATC ACAGGACACA GCTATGTGGT GTATGGACCC 
CTCTGCAATG GAGCTACCAC GGTCCTTTTT GAGAGCACCC CAGTTTACCC TGATGCTGGT 
CGTTACTGGG AGACAGTACA GAGGCTAAAG ATCAACCAGT TCTATGGTGC CCCGACAGCT 
GTCCGACTGC TGCTGAAGTT CGGGGATGCC TGGGTGAAAA AGTATGACCG CTCCTCCCTG 
CGCACACTGG GGTCAGTGGG AGAGCCCATC AATCATGAGG CCTGGGAGTG GCTCCACAAA 
GTGGTGGGTG ATGGCAGATG TACCCTGGTG GACACTTGGT GGCAGACAGA AACTGGAGGC 
ATCTGCATTG CACCACGGCC CTCGGAAGAT GGGGCAGAGA TCCTCCCAGG CATGGCCATG 
AGGCCGTTTT TTGGCATTGT CCCTGCACTC ATGGATGAGA AGGGCAATGT TGTGGAGGGT 
GGTGATGTCT CTGGGGCCCT GTGTATTTCC CAAGCTTGGC CAGGCATGGC AAGGACCATC 
TACGGTGACC ACCAGAGGTT CGTAGATGCC TACTTCAGAA CGTACCCAGG TTACTACTTC 
ACTGGAGATG GAGCTCACCG GACGGAGGGT GGCTATTACC AGATCACAGG GCGCATGGAT 
GATGTCATCA ACATCAGTGG TCATCGCCTG GGGACTGCAG AGATTGAGGA TGCAATGGCT 
GACCATCCCG CTGTCCCAGA AACTGCTGTC ATTGGGTACC CTCATGACAT CAAAGGAGAA 
GCTGCGTTTG CCTTCATTGT ACTGAAAGAT GACGTCCGTG ATGCAAACGT GGTGGTAAAT 
GAGCTCAAGT TGGCAGTGGC CACCAAGATC GCCAAGTATG CTGTGCCTGA CCAGATCCTG 
GTGGTGAAGC GTCTCCCCAA AACCAGGTCT GGGAAGGTGA TGAGGAGGCT GCTGAGGAAG 
ATCATCACCA GCAGGGGGCA GGATCTAGGG GACACCACTA CCCTGGAGGA CCCCAGTGTC 
ATCACGGAGA TCATGAGTGC CTTTCGGGAG TACGAAGAGC AGCGGGCTGC CACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acss1 ( NM_001106524.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099994.1 Rattus norvegicus acyl-CoA synthetase short-chain family member 1 (Acss1), mRNA.

Price & Availability↑

CloneID	ORa01545
Clone ID Related Accession (Same CDS sequence)	NM_001106524.1
Accession Version	NM_001106524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-11
Organism	Rattus norvegicus(Norway rat)
Product	acetyl-coenzyme A synthetase 2-like, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474050.2. On Oct 4, 2007 this sequence version replaced XM_215897.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCGC GCAGCCTCGG CAGCGGTGTG GGGCGACTGC TGCGTGGCCT GCACGGGCAC 
TCGGGACAAT CGGGGTGGTT GCTGAGCCTG TCCCGCTGGA CCACCACCCG ATTCCTGGGC 
AGTGTTCCTG CAGCAGCGCA GCTAGGCTCG TACCCCGCTT TGAGCGCTCA GGCAGCCCAG 
GAGCCGGCCG CCTTCTGGGG ACCGCTGGCC CGTGACACAC TAGTTTGGGA CACTCCTTAC 
CATACTGTCT GGGACTGCGA CTTCAGGACG GGCAAGATCG GCTGGTTCCT GGGAGGCCAG 
TTGAATGTGT CTGTCAACTG CCTGGACCAG CACGTGCAGA AGTCTCCCGA CACCATAGCT 
TTGATTTGGG AGAGAGATGA ACCTGGGACA GAAGTGAGGA TCACTTACAG GGAGCTTCTG 
GAGACCACAT GCCGCCTGGC CAACACGCTG AAGAGGCATG GTGTGCACCG AGGGGACCGT 
GTGGCCATCT ACATGCCTGT GTCCCCACTG GCTGTGGCAG CGATGCTGGC CTGTGCCAGG 
ATTGGAGCTA TCCACACAGT GGTTTTTGCT GGCTTTAGTG CAGAGTCCTT GGCCGGGAGG 
ATCAATGATG CCAAGTGCAA GGTTGTTATC ACCTTCAACC AAGGACTCCG GGGAGGGCGT 
GTGGTGGAGC TGAAGAAAAT AGTGGACGAA GCTGTGAAGA ACTGCCCAAC AGTGCAGCAT 
GTCCTAGTGG CTCACAGGAC GGACACCAAG GTTCCCATGG GGAATCTGGA CATCCCCCTT 
GAGCAGGAGA TGGCCAATGA GGCCCCTGTG TGTACCCCTG AAAGCATGAA CAGTGAAGAT 
ATGCTCTTCA TGCTCTACAC GTCTGGGAGC ACTGGGACAC CCAAGGGACT CGTTCATACA 
CAGGCAGGCT ATCTGCTGTA TGCCGCCATG ACGCACAAGC TCGTGTTTGA CTACCATCCA 
GGTGATGTCT TTGGTTGTGT GGCTGACATC GGTTGGATCA CAGGACACAG CTATGTGGTG 
TATGGACCCC TCTGCAATGG AGCTACCACG GTCCTTTTTG AGAGCACCCC AGTTTACCCT 
GATGCTGGTC GTTACTGGGA GACAGTACAG AGGCTAAAGA TCAACCAGTT CTATGGTGCC 
CCGACAGCTG TCCGACTGCT GCTGAAGTTC GGGGATGCCT GGGTGAAAAA GTATGACCGC 
TCCTCCCTGC GCACACTGGG GTCAGTGGGA GAGCCCATCA ATCATGAGGC CTGGGAGTGG 
CTCCACAAAG TGGTGGGTGA TGGCAGATGT ACCCTGGTGG ACACTTGGTG GCAGACAGAA 
ACTGGAGGCA TCTGCATTGC ACCACGGCCC TCGGAAGATG GGGCAGAGAT CCTCCCAGGC 
ATGGCCATGA GGCCGTTTTT TGGCATTGTC CCTGCACTCA TGGATGAGAA GGGCAATGTT 
GTGGAGGGTG GTGATGTCTC TGGGGCCCTG TGTATTTCCC AAGCTTGGCC AGGCATGGCA 
AGGACCATCT ACGGTGACCA CCAGAGGTTC GTAGATGCCT ACTTCAGAAC GTACCCAGGT 
TACTACTTCA CTGGAGATGG AGCTCACCGG ACGGAGGGTG GCTATTACCA GATCACAGGG 
CGCATGGATG ATGTCATCAA CATCAGTGGT CATCGCCTGG GGACTGCAGA GATTGAGGAT 
GCAATGGCTG ACCATCCCGC TGTCCCAGAA ACTGCTGTCA TTGGGTACCC TCATGACATC 
AAAGGAGAAG CTGCGTTTGC CTTCATTGTA CTGAAAGATG ACGTCCGTGA TGCAAACGTG 
GTGGTAAATG AGCTCAAGTT GGCAGTGGCC ACCAAGATCG CCAAGTATGC TGTGCCTGAC 
CAGATCCTGG TGGTGAAGCG TCTCCCCAAA ACCAGGTCTG GGAAGGTGAT GAGGAGGCTG 
CTGAGGAAGA TCATCACCAG CAGGGGGCAG GATCTAGGGG ACACCACTAC CCTGGAGGAC 
CCCAGTGTCA TCACGGAGAT CATGAGTGCC TTTCGGGAGT ACGAAGAGCA GCGGGCTGCC 
ACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099994.1
CDS	33..2081
Translation	MAARSLGSGVGRLLRGLHGHSGQSGWLLSLSRWTTTRFLGSVPAAAQLGSYPALSAQAAQEPAAFWGPLARDTLVWDTPYHTVWDCDFRTGKIGWFLGGQLNVSVNCLDQHVQKSPDTIALIWERDEPGTEVRITYRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAIHTVVFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKNCPTVQHVLVAHRTDTKVPMGNLDIPLEQEMANEAPVCTPESMNSEDMLFMLYTSGSTGTPKGLVHTQAGYLLYAAMTHKLVFDYHPGDVFGCVADIGWITGHSYVVYGPLCNGATTVLFESTPVYPDAGRYWETVQRLKINQFYGAPTAVRLLLKFGDAWVKKYDRSSLRTLGSVGEPINHEAWEWLHKVVGDGRCTLVDTWWQTETGGICIAPRPSEDGAEILPGMAMRPFFGIVPALMDEKGNVVEGGDVSGALCISQAWPGMARTIYGDHQRFVDAYFRTYPGYYFTGDGAHRTEGGYYQITGRMDDVINISGHRLGTAEIEDAMADHPAVPETAVIGYPHDIKGEAAFAFIVLKDDVRDANVVVNELKLAVATKIAKYAVPDQILVVKRLPKTRSGKVMRRLLRKIITSRGQDLGDTTTLEDPSVITEIMSAFREYEEQRAATN

Target ORF information:

RefSeq Version	NM_001106524.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase short-chain family member 1 (Acss1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106524.1

ORF Insert Sequence:

ATGGCGGCGC GCAGCCTCGG CAGCGGTGTG GGGCGACTGC TGCGTGGCCT GCACGGGCAC 
TCGGGACAAT CGGGGTGGTT GCTGAGCCTG TCCCGCTGGA CCACCACCCG ATTCCTGGGC 
AGTGTTCCTG CAGCAGCGCA GCTAGGCTCG TACCCCGCTT TGAGCGCTCA GGCAGCCCAG 
GAGCCGGCCG CCTTCTGGGG ACCGCTGGCC CGTGACACAC TAGTTTGGGA CACTCCTTAC 
CATACTGTCT GGGACTGCGA CTTCAGGACG GGCAAGATCG GCTGGTTCCT GGGAGGCCAG 
TTGAATGTGT CTGTCAACTG CCTGGACCAG CACGTGCAGA AGTCTCCCGA CACCATAGCT 
TTGATTTGGG AGAGAGATGA ACCTGGGACA GAAGTGAGGA TCACTTACAG GGAGCTTCTG 
GAGACCACAT GCCGCCTGGC CAACACGCTG AAGAGGCATG GTGTGCACCG AGGGGACCGT 
GTGGCCATCT ACATGCCTGT GTCCCCACTG GCTGTGGCAG CGATGCTGGC CTGTGCCAGG 
ATTGGAGCTA TCCACACAGT GGTTTTTGCT GGCTTTAGTG CAGAGTCCTT GGCCGGGAGG 
ATCAATGATG CCAAGTGCAA GGTTGTTATC ACCTTCAACC AAGGACTCCG GGGAGGGCGT 
GTGGTGGAGC TGAAGAAAAT AGTGGACGAA GCTGTGAAGA ACTGCCCAAC AGTGCAGCAT 
GTCCTAGTGG CTCACAGGAC GGACACCAAG GTTCCCATGG GGAATCTGGA CATCCCCCTT 
GAGCAGGAGA TGGCCAATGA GGCCCCTGTG TGTACCCCTG AAAGCATGAA CAGTGAAGAT 
ATGCTCTTCA TGCTCTACAC GTCTGGGAGC ACTGGGACAC CCAAGGGACT CGTTCATACA 
CAGGCAGGCT ATCTGCTGTA TGCCGCCATG ACGCACAAGC TCGTGTTTGA CTACCATCCA 
GGTGATGTCT TTGGTTGTGT GGCTGACATC GGTTGGATCA CAGGACACAG CTATGTGGTG 
TATGGACCCC TCTGCAATGG AGCTACCACG GTCCTTTTTG AGAGCACCCC AGTTTACCCT 
GATGCTGGTC GTTACTGGGA GACAGTACAG AGGCTAAAGA TCAACCAGTT CTATGGTGCC 
CCGACAGCTG TCCGACTGCT GCTGAAGTTC GGGGATGCCT GGGTGAAAAA GTATGACCGC 
TCCTCCCTGC GCACACTGGG GTCAGTGGGA GAGCCCATCA ATCATGAGGC CTGGGAGTGG 
CTCCACAAAG TGGTGGGTGA TGGCAGATGT ACCCTGGTGG ACACTTGGTG GCAGACAGAA 
ACTGGAGGCA TCTGCATTGC ACCACGGCCC TCGGAAGATG GGGCAGAGAT CCTCCCAGGC 
ATGGCCATGA GGCCGTTTTT TGGCATTGTC CCTGCACTCA TGGATGAGAA GGGCAATGTT 
GTGGAGGGTG GTGATGTCTC TGGGGCCCTG TGTATTTCCC AAGCTTGGCC AGGCATGGCA 
AGGACCATCT ACGGTGACCA CCAGAGGTTC GTAGATGCCT ACTTCAGAAC GTACCCAGGT 
TACTACTTCA CTGGAGATGG AGCTCACCGG ACGGAGGGTG GCTATTACCA GATCACAGGG 
CGCATGGATG ATGTCATCAA CATCAGTGGT CATCGCCTGG GGACTGCAGA GATTGAGGAT 
GCAATGGCTG ACCATCCCGC TGTCCCAGAA ACTGCTGTCA TTGGGTACCC TCATGACATC 
AAAGGAGAAG CTGCGTTTGC CTTCATTGTA CTGAAAGATG ACGTCCGTGA TGCAAACGTG 
GTGGTAAATG AGCTCAAGTT GGCAGTGGCC ACCAAGATCG CCAAGTATGC TGTGCCTGAC 
CAGATCCTGG TGGTGAAGCG TCTCCCCAAA ACCAGGTCTG GGAAGGTGAT GAGGAGGCTG 
CTGAGGAAGA TCATCACCAG CAGGGGGCAG GATCTAGGGG ACACCACTAC CCTGGAGGAC 
CCCAGTGTCA TCACGGAGAT CATGAGTGCC TTTCGGGAGT ACGAAGAGCA GCGGGCTGCC 
ACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glucose-independent Acetate Metabolism Promotes Melanoma Cell Survival and Tumor Growth.
The Journal of biological chemistry291(42)21869-21879(2016 Oct)
Lakhter AJ,Hamilton J,Konger RL,Brustovetsky N,Broxmeyer HE,Naidu SR

Fasting-induced hypothermia and reduced energy production in mice lacking acetyl-CoA synthetase 2.
Cell metabolism9(2)191-202(2009 Feb)
Sakakibara I,Fujino T,Ishii M,Tanaka T,Shimosawa T,Miura S,Zhang W,Tokutake Y,Yamamoto J,Awano M,Iwasaki S,Motoike T,Okamura M,Inagaki T,Kita K,Ezaki O,Naito M,Kuwaki T,Chohnan S,Yamamoto TT,Hammer RE,Kodama T,Yanagisawa M,Sakai J

Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome.
Journal of lipid research48(12)2736-50(2007 Dec)
Watkins PA,Maiguel D,Jia Z,Pevsner J

Reversible lysine acetylation controls the activity of the mitochondrial enzyme acetyl-CoA synthetase 2.
Proceedings of the National Academy of Sciences of the United States of America103(27)10224-9(2006 Jul)
Schwer B,Bunkenborg J,Verdin RO,Andersen JS,Verdin E

Acetyl-CoA synthetase 2, a mitochondrial matrix enzyme involved in the oxidation of acetate.
The Journal of biological chemistry276(14)11420-6(2001 Apr)
Fujino T,Kondo J,Ishikawa M,Morikawa K,Yamamoto TT

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Acss1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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