PROK1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PROK1
Entrez Gene ID	84432
Full Name	prokineticin 1
Synonyms	EGVEGF,PK1,PRK1
General protein information	Preferred Names prokineticin 1 Names prokineticin-1 EG-VEGF black mamba toxin-related protein endocrine-gland-derived vascular endothelial growth factor mambakine
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p13.3
Summary	The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011].
Disorder MIM:	606233

mRNA and Protein(s)

mRNA	Protein	Name
NM_032414.2	NP_115790.1	prokineticin-1 precursor
NM_032414.3	NP_115790.1	prokineticin-1 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	prok1	NP_001099168.1
Bos taurus (cattle)	PROK1	NP_001029190.1
Homo sapiens (human)	PROK1	NP_115790.1
Gallus gallus (chicken)	PROK1	XP_425816.1
	PROK1	XP_001101188.1
Mus musculus (house mouse)	Prok1	NP_001037847.1
Rattus norvegicus (Norway rat)	Prok1	NP_620206.1
Pan troglodytes (chimpanzee)	PROK1	XP_001162989.1
Canis lupus familiaris (dog)	PROK1	XP_003639164.1
Xenopus tropicalis (tropical clawed frog)	prok1	XP_002933044.1

Gene Ontology
Bibliography

Related articles in PubMed

Prokineticin 1 is up-regulated by insulin in decidualizing human endometrial stromal cells.
Ujvari D, Jakson I, Oldmark C, Attarha S, Alkasalias T, Salamon D, Gidl?f S, Hirschberg AL
Journal of cellular and molecular medicine22(1)163-172(2018 Jan)

High levels of soluble vascular endothelial growth factor receptor 1/sFlt1 and low levels of vascular endothelial growth factor in follicular fluid on the day of oocyte retrieval correlate with ovarian hyperstimulation syndrom regardless of the stimulation protocol.
Jakimiuk AJ, Nowicka MA, Zagozda M, Koziol K, Lewandowski P, Issat T
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society68(3)477-484(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings demonstrate a novel function of primary cilia in controlling EG-VEGF-regulated trophoblast invasion and reveal the underlying molecular mechanism.
Title: Primary Cilium-Regulated EG-VEGF Signaling Facilitates Trophoblast Invasion.

miR-346 and miR-582-3p regulate EG-VEGF-induced trophoblast invasion through repressing MMP 2 and MMP 9, and may become novel diagnostic biomarkers or therapeutic targets for EG-VEGF-related obstetric disorders. (c) 2016 BioFactors, 43(2):210-219, 2017.
Title: miR-346 and miR-582-3p-regulated EG-VEGF expression and trophoblast invasion via matrix metalloproteinases 2 and 9.

Each follicular fluid (FF) was individually aspirated and FF/serum EG-VEGF, inhibin-a, and FF IGF-1 levels were evaluated. The pregnant group was characterized by increased numbers of WVFs (p = 0.044), a WVE (p = 0.022), and increased levels of FF IGF-1 (p = 0.001) and serum EG-VEGF (p = 0.03).
Title: Perifollicular blood flow and its relationship with endometrial vascularity, follicular fluid EG-VEGF, IGF-1, and inhibin-a levels and IVF outcomes.

Results show the biological effects of PROK1-V67I on cell functions are similar to those of wild type, and the common variant of V67I may act as a modifier in the PROK1-PROKR system through down-regulation of PROK1 expression.
Title: A Common Variant of PROK1 (V67I) Acts as a Genetic Modifier in Early Human Pregnancy through Down-Regulation of Gene Expression.

Data suggest that prokineticins (PROK1 and PROK2) and prokineticin receptors (PROKR1 and PROKR2) act as main regulators of physiological functions of ovary, uterus, placenta, and testis. [REVIEW]
Title: Prokineticins in central and peripheral control of human reproduction.

The following PROK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PROK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23344	NM_032414.3 Latest version!	Homo sapiens prokineticin 1 (PROK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu23344	NM_032414.2	Homo sapiens prokineticin 1 (PROK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PROK1 ( NM_032414.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_115790.1 Homo sapiens prokineticin 1 (PROK1), mRNA.

Price & Availability↑

CloneID	OHu23344
Clone ID Related Accession (Same CDS sequence)	NM_032414.2 , NM_032414.3
Accession Version	NM_032414.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-25
Organism	Homo sapiens(human)
Product	prokineticin-1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM544315.1 and AY029225.1. On Dec 16, 2004 this sequence version replaced NM_032414.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358683.1, AF333024.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

ATGAGAGGTG CCACGCGAGT CTCAATCATG CTCCTCCTAG TAACTGTGTC TGACTGTGCT 
GTGATCACAG GGGCCTGTGA GCGGGATGTC CAGTGTGGGG CAGGCACCTG CTGTGCCATC 
AGCCTGTGGC TTCGAGGGCT GCGGATGTGC ACCCCGCTGG GGCGGGAAGG CGAGGAGTGC 
CACCCCGGCA GCCACAAGGT CCCCTTCTTC AGGAAACGCA AGCACCACAC CTGTCCTTGC 
TTGCCCAACC TGCTGTGCTC CAGGTTCCCG GACGGCAGGT ACCGCTGCTC CATGGACTTG 
AAGAACATCA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115790.1
CDS	52..369
Translation	MRGATRVSIMLLLVTVSDCAVITGACERDVQCGAGTCCAISLWLRGLRMCTPLGREGEECHPGSHKVPFFRKRKHHTCPCLPNLLCSRFPDGRYRCSMDLKNINF

Target ORF information:

RefSeq Version	NM_032414.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prokineticin 1 (PROK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032414.2

ORF Insert Sequence:

ATGAGAGGTG CCACGCGAGT CTCAATCATG CTCCTCCTAG TAACTGTGTC TGACTGTGCT 
GTGATCACAG GGGCCTGTGA GCGGGATGTC CAGTGTGGGG CAGGCACCTG CTGTGCCATC 
AGCCTGTGGC TTCGAGGGCT GCGGATGTGC ACCCCGCTGG GGCGGGAAGG CGAGGAGTGC 
CACCCCGGCA GCCACAAGGT CCCCTTCTTC AGGAAACGCA AGCACCACAC CTGTCCTTGC 
TTGCCCAACC TGCTGTGCTC CAGGTTCCCG GACGGCAGGT ACCGCTGCTC CATGGACTTG 
AAGAACATCA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PROK1 ( NM_032414.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115790.1 Homo sapiens prokineticin 1 (PROK1), mRNA.

Price & Availability↑

CloneID	OHu23344
Clone ID Related Accession (Same CDS sequence)	NM_032414.2 , NM_032414.3
Accession Version	NM_032414.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	prokineticin-1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL390797.16, BM544315.1, AY029225.1 and AL358215.16. On Nov 23, 2018 this sequence version replaced NM_032414.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.162299.1, SRR5189667.173366.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000271331.4/ ENSP00000271331.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGAGGTG CCACGCGAGT CTCAATCATG CTCCTCCTAG TAACTGTGTC TGACTGTGCT 
GTGATCACAG GGGCCTGTGA GCGGGATGTC CAGTGTGGGG CAGGCACCTG CTGTGCCATC 
AGCCTGTGGC TTCGAGGGCT GCGGATGTGC ACCCCGCTGG GGCGGGAAGG CGAGGAGTGC 
CACCCCGGCA GCCACAAGGT CCCCTTCTTC AGGAAACGCA AGCACCACAC CTGTCCTTGC 
TTGCCCAACC TGCTGTGCTC CAGGTTCCCG GACGGCAGGT ACCGCTGCTC CATGGACTTG 
AAGAACATCA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115790.1
CDS	69..386
Translation	MRGATRVSIMLLLVTVSDCAVITGACERDVQCGAGTCCAISLWLRGLRMCTPLGREGEECHPGSHKVPFFRKRKHHTCPCLPNLLCSRFPDGRYRCSMDLKNINF

Target ORF information:

RefSeq Version	NM_032414.3
Organism	Homo sapiens(human)
Definition	Homo sapiens prokineticin 1 (PROK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032414.3

ORF Insert Sequence:

ATGAGAGGTG CCACGCGAGT CTCAATCATG CTCCTCCTAG TAACTGTGTC TGACTGTGCT 
GTGATCACAG GGGCCTGTGA GCGGGATGTC CAGTGTGGGG CAGGCACCTG CTGTGCCATC 
AGCCTGTGGC TTCGAGGGCT GCGGATGTGC ACCCCGCTGG GGCGGGAAGG CGAGGAGTGC 
CACCCCGGCA GCCACAAGGT CCCCTTCTTC AGGAAACGCA AGCACCACAC CTGTCCTTGC 
TTGCCCAACC TGCTGTGCTC CAGGTTCCCG GACGGCAGGT ACCGCTGCTC CATGGACTTG 
AAGAACATCA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prokineticin 1 is up-regulated by insulin in decidualizing human endometrial stromal cells.
Journal of cellular and molecular medicine22(1)163-172(2018 Jan)
Ujvari D,Jakson I,Oldmark C,Attarha S,Alkasalias T,Salamon D,Gidl?f S,Hirschberg AL

High levels of soluble vascular endothelial growth factor receptor 1/sFlt1 and low levels of vascular endothelial growth factor in follicular fluid on the day of oocyte retrieval correlate with ovarian hyperstimulation syndrom regardless of the stimulation protocol.
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society68(3)477-484(2017 Jun)
Jakimiuk AJ,Nowicka MA,Zagozda M,Koziol K,Lewandowski P,Issat T

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PROK1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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