CAPN3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAPN3
Entrez Gene ID	825
Full Name	calpain 3
Synonyms	CANP3,CANPL3,LGMD2,LGMD2A,nCL-1,p94
General protein information	Preferred Names calpain 3 Names calpain-3 calpain p94, large [catalytic] subunit calpain, large polypeptide L3 muscle-specific calcium-activated neutral protease 3 large subunit new calpain 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q15.1
Summary	Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq, Jul 2008].
Disorder MIM:	114240
Disorder Html:	Muscular dystrophy, limb-girdle, type 2A, 253600 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000070.3	NP_000061.1	calpain-3 isoform a
NM_000070.2	NP_000061.1	calpain-3 isoform a
NM_173089.1	NP_775112.1	calpain-3 isoform e
NM_173088.1	NP_775111.1	calpain-3 isoform d
NM_173087.1	NP_775110.1	calpain-3 isoform c
NM_173090.1	NP_775113.1	calpain-3 isoform e
NM_024344.1	NP_077320.1	calpain-3 isoform b

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100497532	XP_002935381.2
Homo sapiens (human)	CAPN3	NP_000061.1
Mus musculus (house mouse)	Capn3	NP_031627.2
Rattus norvegicus (Norway rat)	Capn3	NP_058813.1
Canis lupus familiaris (dog)	CAPN3	XP_005638349.1
Gallus gallus (chicken)	CAPN3	NP_001004405.1
	CAPN3	XP_001103220.1
Danio rerio (zebrafish)	capn3a	NP_001004571.1
Bos taurus (cattle)	CAPN3	NP_776685.1

Gene Ontology
Bibliography

Related articles in PubMed

Autosomal dominant calpainopathy due to heterozygous CAPN3 c.643_663del21.
Martinez-Thompson JM, Niu Z, Tracy JA, Moore SA, Swenson A, Wieben ED, Milone M
Muscle & nerve(2017 Sep)

Integrative variants, haplotypes and diplotypes of the CAPN3 and FRMD5 genes and several environmental exposures associate with serum lipid variables.
Guo T, Yin RX, Pan L, Yang S, Miao L, Huang F
Scientific reports745119(2017 Mar)

Epidemiological and Molecular Characterization of a Mexican Population Isolate with High Prevalence of Limb-Girdle Muscular Dystrophy Type 2A Due to a Novel Calpain-3 Mutation.
Pantoja-Melendez CA, Miranda-Duarte A, Roque-Ramirez B, Zenteno JC
PloS one12(1)e0170280(2017)

Novel Homozygous Missense Mutation in CAPN3 Gene Detected in a Saudi Arabian Family With Limb-Girdle Muscular Dystrophy Type 2A.
Al-Harbi TM, Abdulmana? SO, Dridi W
Journal of clinical neuromuscular disease18(2)89-91(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrates that a cluster of patients with Limb-Girdle Muscular Dystrophy Type 2A in a small Mexican village arises from a novel CAPN3 founder mutation.
Title: Epidemiological and Molecular Characterization of a Mexican Population Isolate with High Prevalence of Limb-Girdle Muscular Dystrophy Type 2A Due to a Novel Calpain-3 Mutation.

Here, for the first time, we report a new variant in the CAPN3 gene that can be considered as a robust genetics factor causing limb-girdle muscular dystrophy type 2A disease.
Title: Novel Homozygous Missense Mutation in CAPN3 Gene Detected in a Saudi Arabian Family With Limb-Girdle Muscular Dystrophy Type 2A.

Heterozygosity for c.643_663del21 in CAPN3 results in a myopathy resembling the recessive form.
Title: A heterozygous 21-bp deletion in CAPN3 causes dominantly inherited limb girdle muscular dystrophy.

Unrelated families with limb girdle muscular dystrophy shared common haplotypes with homozygote patterns in two families, and a compound heterozygote pattern in the third family.
Title: Linkage Study Revealed Complex Haplotypes in a Multifamily due to Different Mutations in CAPN3 Gene in an ian Ethnic Group.

The allele frequency of CAPN3 gene mutation in limb-girdle muscular dystrophy patients was different in patients from Latvia and Lithuania.
Title: Robust genotyping tool for autosomal recessive type of limb-girdle muscular dystrophies.

The following CAPN3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAPN3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28271	NM_000070.3 Latest version!	Homo sapiens calpain 3 (CAPN3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu28275	NM_173087.1 Latest version!	Homo sapiens calpain 3 (CAPN3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu28277	NM_173088.1 Latest version!	Homo sapiens calpain 3 (CAPN3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu28285	NM_173089.1 Latest version!	Homo sapiens calpain 3 (CAPN3), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu28285	NM_173090.1 Latest version!	Homo sapiens calpain 3 (CAPN3), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu28286	NM_024344.1 Latest version!	Homo sapiens calpain 3 (CAPN3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu28271	NM_000070.2	Homo sapiens calpain 3 (CAPN3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAPN3 ( NM_000070.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000061.1 Homo sapiens calpain 3 (CAPN3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28271
Clone ID Related Accession (Same CDS sequence)	NM_000070.3 , NM_000070.2
Accession Version	NM_000070.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	calpain-3 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012651.8. This sequence is a reference standard in the RefSeqGene project. On Nov 26, 2018 this sequence version replaced NM_000070.2. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.88161.1, SRR1803615.236543.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000397163.8/ ENSP00000380349.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGACCG TCATTAGCGC ATCTGTGGCT CCAAGGACAG CGGCTGAGCC CCGGTCCCCA 
GGGCCAGTTC CTCACCCGGC CCAGAGCAAG GCCACTGAGG CTGGGGGTGG AAACCCAAGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCTATTA TCGGAGTGAA AGAGAAGACA 
TTCGAGCAAC TTCACAAGAA ATGTCTAGAA AAGAAAGTTC TTTATGTGGA CCCTGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTATAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGCGA GAATCCCCGA TTTATCATTG ATGGAGCCAA CAGAACTGAC 
ATCTGTCAAG GAGAGCTAGG GGACTGCTGG TTTCTCGCAG CCATTGCCTG CCTGACCCTG 
AACCAGCACC TTCTTTTCCG AGTCATACCC CATGATCAAA GTTTCATCGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGAGTGGG TGGACGTGGT TATAGATGAC 
TGCCTGCCAA CGTACAACAA TCAACTGGTT TTCACCAAGT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCCTACGA AGCTCTGAAA 
GGTGGGAACA CCACAGAGGC CATGGAGGAC TTCACAGGAG GGGTGGCAGA GTTTTTTGAG 
ATCAGGGATG CTCCTAGTGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACG AACATGACCT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAGTTGAT TGCACGGATG GTAAGGAATA TGGATAACTC ACTGCTCCAG 
GACTCAGACC TCGACCCCAG AGGCTCAGAT GAAAGACCGA CCCGGACAAT CATTCCGGTT 
CAGTATGAGA CAAGAATGGC CTGCGGGCTG GTCAGAGGTC ACGCCTACTC TGTCACGGGG 
CTGGATGAGG TCCCGTTCAA AGGTGAGAAA GTGAAGCTGG TGCGGCTGCG GAATCCGTGG 
GGCCAGGTGG AGTGGAACGG TTCTTGGAGT GATAGATGGA AGGACTGGAG CTTTGTGGAC 
AAAGATGAGA AGGCCCGTCT GCAGCACCAG GTCACTGAGG ATGGAGAGTT CTGGATGTCC 
TATGAGGATT TCATCTACCA TTTCACAAAG TTGGAGATCT GCAACCTCAC GGCCGATGCT 
CTGCAGTCTG ACAAGCTTCA GACCTGGACA GTGTCTGTGA ACGAGGGCCG CTGGGTACGG 
GGTTGCTCTG CCGGAGGCTG CCGCAACTTC CCAGATACTT TCTGGACCAA CCCTCAGTAC 
CGTCTGAAGC TCCTGGAGGA GGACGATGAC CCTGATGACT CGGAGGTGAT TTGCAGCTTC 
CTGGTGGCCC TGATGCAGAA GAACCGGCGG AAGGACCGGA AGCTAGGGGC CAGTCTCTTC 
ACCATTGGCT TCGCCATCTA CGAGGTTCCC AAAGAGATGC ACGGGAACAA GCAGCACCTG 
CAGAAGGACT TCTTCCTGTA CAACGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCT CCCAGCGAGT ACGTCATCGT GCCCTCCACC 
TACGAGCCCC ACCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAACCTC 
TCTGAGGAAG TTGAAAATAC CATCTCCGTG GATCGGCCAG TGAAAAAGAA AAAAACCAAG 
CCCATCATCT TCGTTTCGGA CAGAGCAAAC AGCAACAAGG AGCTGGGTGT GGACCAGGAG 
TCAGAGGAGG GCAAAGGCAA AACAAGCCCT GATAAGCAAA AGCAGTCCCC ACAGCCACAG 
CCTGGCAGCT CTGATCAGGA AAGTGAGGAA CAGCAACAAT TCCGGAACAT TTTCAAGCAG 
ATAGCAGGAG ATGACATGGA GATCTGTGCA GATGAGCTCA AGAAGGTCCT TAACACAGTC 
GTGAACAAAC ACAAGGACCT GAAGACACAC GGGTTCACAC TGGAGTCCTG CCGTAGCATG 
ATTGCGCTCA TGGATACAGA TGGCTCTGGA AAGCTCAACC TGCAGGAGTT CCACCACCTC 
TGGAACAAGA TTAAGGCCTG GCAGAAAATT TTCAAACACT ATGACACAGA CCAGTCCGGC 
ACCATCAACA GCTACGAGAT GCGAAATGCA GTCAACGACG CAGGATTCCA CCTCAACAAC 
CAGCTCTATG ACATCATTAC CATGCGGTAC GCAGACAAAC ACATGAACAT CGACTTTGAC 
AGTTTCATCT GCTGCTTCGT TAGGCTGGAG GGCATGTTCA GAGCTTTTCA TGCATTTGAC 
AAGGATGGAG ATGGTATCAT CAAGCTCAAC GTTCTGGAGT GGCTGCAGCT CACCATGTAT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000061.1
CDS	306..2771
Translation	MPTVISASVAPRTAAEPRSPGPVPHPAQSKATEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVAEFFEIRDAPSDMYKIMKKAIERGSLMGCSIDDGTNMTYGTSPSGLNMGELIARMVRNMDNSLLQDSDLDPRGSDERPTRTIIPVQYETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQVEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGASLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVKKKKTKPIIFVSDRANSNKELGVDQESEEGKGKTSPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQIAGDDMEICADELKKVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	NM_000070.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 3 (CAPN3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000070.3

ORF Insert Sequence:

ATGCCGACCG TCATTAGCGC ATCTGTGGCT CCAAGGACAG CGGCTGAGCC CCGGTCCCCA 
GGGCCAGTTC CTCACCCGGC CCAGAGCAAG GCCACTGAGG CTGGGGGTGG AAACCCAAGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCTATTA TCGGAGTGAA AGAGAAGACA 
TTCGAGCAAC TTCACAAGAA ATGTCTAGAA AAGAAAGTTC TTTATGTGGA CCCTGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTATAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGCGA GAATCCCCGA TTTATCATTG ATGGAGCCAA CAGAACTGAC 
ATCTGTCAAG GAGAGCTAGG GGACTGCTGG TTTCTCGCAG CCATTGCCTG CCTGACCCTG 
AACCAGCACC TTCTTTTCCG AGTCATACCC CATGATCAAA GTTTCATCGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGAGTGGG TGGACGTGGT TATAGATGAC 
TGCCTGCCAA CGTACAACAA TCAACTGGTT TTCACCAAGT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCCTACGA AGCTCTGAAA 
GGTGGGAACA CCACAGAGGC CATGGAGGAC TTCACAGGAG GGGTGGCAGA GTTTTTTGAG 
ATCAGGGATG CTCCTAGTGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACG AACATGACCT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAGTTGAT TGCACGGATG GTAAGGAATA TGGATAACTC ACTGCTCCAG 
GACTCAGACC TCGACCCCAG AGGCTCAGAT GAAAGACCGA CCCGGACAAT CATTCCGGTT 
CAGTATGAGA CAAGAATGGC CTGCGGGCTG GTCAGAGGTC ACGCCTACTC TGTCACGGGG 
CTGGATGAGG TCCCGTTCAA AGGTGAGAAA GTGAAGCTGG TGCGGCTGCG GAATCCGTGG 
GGCCAGGTGG AGTGGAACGG TTCTTGGAGT GATAGATGGA AGGACTGGAG CTTTGTGGAC 
AAAGATGAGA AGGCCCGTCT GCAGCACCAG GTCACTGAGG ATGGAGAGTT CTGGATGTCC 
TATGAGGATT TCATCTACCA TTTCACAAAG TTGGAGATCT GCAACCTCAC GGCCGATGCT 
CTGCAGTCTG ACAAGCTTCA GACCTGGACA GTGTCTGTGA ACGAGGGCCG CTGGGTACGG 
GGTTGCTCTG CCGGAGGCTG CCGCAACTTC CCAGATACTT TCTGGACCAA CCCTCAGTAC 
CGTCTGAAGC TCCTGGAGGA GGACGATGAC CCTGATGACT CGGAGGTGAT TTGCAGCTTC 
CTGGTGGCCC TGATGCAGAA GAACCGGCGG AAGGACCGGA AGCTAGGGGC CAGTCTCTTC 
ACCATTGGCT TCGCCATCTA CGAGGTTCCC AAAGAGATGC ACGGGAACAA GCAGCACCTG 
CAGAAGGACT TCTTCCTGTA CAACGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCT CCCAGCGAGT ACGTCATCGT GCCCTCCACC 
TACGAGCCCC ACCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAACCTC 
TCTGAGGAAG TTGAAAATAC CATCTCCGTG GATCGGCCAG TGAAAAAGAA AAAAACCAAG 
CCCATCATCT TCGTTTCGGA CAGAGCAAAC AGCAACAAGG AGCTGGGTGT GGACCAGGAG 
TCAGAGGAGG GCAAAGGCAA AACAAGCCCT GATAAGCAAA AGCAGTCCCC ACAGCCACAG 
CCTGGCAGCT CTGATCAGGA AAGTGAGGAA CAGCAACAAT TCCGGAACAT TTTCAAGCAG 
ATAGCAGGAG ATGACATGGA GATCTGTGCA GATGAGCTCA AGAAGGTCCT TAACACAGTC 
GTGAACAAAC ACAAGGACCT GAAGACACAC GGGTTCACAC TGGAGTCCTG CCGTAGCATG 
ATTGCGCTCA TGGATACAGA TGGCTCTGGA AAGCTCAACC TGCAGGAGTT CCACCACCTC 
TGGAACAAGA TTAAGGCCTG GCAGAAAATT TTCAAACACT ATGACACAGA CCAGTCCGGC 
ACCATCAACA GCTACGAGAT GCGAAATGCA GTCAACGACG CAGGATTCCA CCTCAACAAC 
CAGCTCTATG ACATCATTAC CATGCGGTAC GCAGACAAAC ACATGAACAT CGACTTTGAC 
AGTTTCATCT GCTGCTTCGT TAGGCTGGAG GGCATGTTCA GAGCTTTTCA TGCATTTGAC 
AAGGATGGAG ATGGTATCAT CAAGCTCAAC GTTCTGGAGT GGCTGCAGCT CACCATGTAT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN3 ( NM_000070.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000061.1 Homo sapiens calpain 3 (CAPN3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28271
Clone ID Related Accession (Same CDS sequence)	NM_000070.3 , NM_000070.2
Accession Version	NM_000070.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	calpain-3 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF209502.1 and BC003169.1. This sequence is a reference standard in the RefSeqGene project. On Jan 16, 2003 this sequence version replaced NM_000070.1. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.88161.1, SRR1803615.236543.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGACCG TCATTAGCGC ATCTGTGGCT CCAAGGACAG CGGCTGAGCC CCGGTCCCCA 
GGGCCAGTTC CTCACCCGGC CCAGAGCAAG GCCACTGAGG CTGGGGGTGG AAACCCAAGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCTATTA TCGGAGTGAA AGAGAAGACA 
TTCGAGCAAC TTCACAAGAA ATGTCTAGAA AAGAAAGTTC TTTATGTGGA CCCTGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTATAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGCGA GAATCCCCGA TTTATCATTG ATGGAGCCAA CAGAACTGAC 
ATCTGTCAAG GAGAGCTAGG GGACTGCTGG TTTCTCGCAG CCATTGCCTG CCTGACCCTG 
AACCAGCACC TTCTTTTCCG AGTCATACCC CATGATCAAA GTTTCATCGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGAGTGGG TGGACGTGGT TATAGATGAC 
TGCCTGCCAA CGTACAACAA TCAACTGGTT TTCACCAAGT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCCTACGA AGCTCTGAAA 
GGTGGGAACA CCACAGAGGC CATGGAGGAC TTCACAGGAG GGGTGGCAGA GTTTTTTGAG 
ATCAGGGATG CTCCTAGTGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACG AACATGACCT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAGTTGAT TGCACGGATG GTAAGGAATA TGGATAACTC ACTGCTCCAG 
GACTCAGACC TCGACCCCAG AGGCTCAGAT GAAAGACCGA CCCGGACAAT CATTCCGGTT 
CAGTATGAGA CAAGAATGGC CTGCGGGCTG GTCAGAGGTC ACGCCTACTC TGTCACGGGG 
CTGGATGAGG TCCCGTTCAA AGGTGAGAAA GTGAAGCTGG TGCGGCTGCG GAATCCGTGG 
GGCCAGGTGG AGTGGAACGG TTCTTGGAGT GATAGATGGA AGGACTGGAG CTTTGTGGAC 
AAAGATGAGA AGGCCCGTCT GCAGCACCAG GTCACTGAGG ATGGAGAGTT CTGGATGTCC 
TATGAGGATT TCATCTACCA TTTCACAAAG TTGGAGATCT GCAACCTCAC GGCCGATGCT 
CTGCAGTCTG ACAAGCTTCA GACCTGGACA GTGTCTGTGA ACGAGGGCCG CTGGGTACGG 
GGTTGCTCTG CCGGAGGCTG CCGCAACTTC CCAGATACTT TCTGGACCAA CCCTCAGTAC 
CGTCTGAAGC TCCTGGAGGA GGACGATGAC CCTGATGACT CGGAGGTGAT TTGCAGCTTC 
CTGGTGGCCC TGATGCAGAA GAACCGGCGG AAGGACCGGA AGCTAGGGGC CAGTCTCTTC 
ACCATTGGCT TCGCCATCTA CGAGGTTCCC AAAGAGATGC ACGGGAACAA GCAGCACCTG 
CAGAAGGACT TCTTCCTGTA CAACGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCT CCCAGCGAGT ACGTCATCGT GCCCTCCACC 
TACGAGCCCC ACCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAACCTC 
TCTGAGGAAG TTGAAAATAC CATCTCCGTG GATCGGCCAG TGAAAAAGAA AAAAACCAAG 
CCCATCATCT TCGTTTCGGA CAGAGCAAAC AGCAACAAGG AGCTGGGTGT GGACCAGGAG 
TCAGAGGAGG GCAAAGGCAA AACAAGCCCT GATAAGCAAA AGCAGTCCCC ACAGCCACAG 
CCTGGCAGCT CTGATCAGGA AAGTGAGGAA CAGCAACAAT TCCGGAACAT TTTCAAGCAG 
ATAGCAGGAG ATGACATGGA GATCTGTGCA GATGAGCTCA AGAAGGTCCT TAACACAGTC 
GTGAACAAAC ACAAGGACCT GAAGACACAC GGGTTCACAC TGGAGTCCTG CCGTAGCATG 
ATTGCGCTCA TGGATACAGA TGGCTCTGGA AAGCTCAACC TGCAGGAGTT CCACCACCTC 
TGGAACAAGA TTAAGGCCTG GCAGAAAATT TTCAAACACT ATGACACAGA CCAGTCCGGC 
ACCATCAACA GCTACGAGAT GCGAAATGCA GTCAACGACG CAGGATTCCA CCTCAACAAC 
CAGCTCTATG ACATCATTAC CATGCGGTAC GCAGACAAAC ACATGAACAT CGACTTTGAC 
AGTTTCATCT GCTGCTTCGT TAGGCTGGAG GGCATGTTCA GAGCTTTTCA TGCATTTGAC 
AAGGATGGAG ATGGTATCAT CAAGCTCAAC GTTCTGGAGT GGCTGCAGCT CACCATGTAT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000061.1
CDS	307..2772
Translation	MPTVISASVAPRTAAEPRSPGPVPHPAQSKATEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVAEFFEIRDAPSDMYKIMKKAIERGSLMGCSIDDGTNMTYGTSPSGLNMGELIARMVRNMDNSLLQDSDLDPRGSDERPTRTIIPVQYETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQVEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGASLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVKKKKTKPIIFVSDRANSNKELGVDQESEEGKGKTSPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQIAGDDMEICADELKKVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	NM_000070.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 3 (CAPN3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000070.2

ORF Insert Sequence:

ATGCCGACCG TCATTAGCGC ATCTGTGGCT CCAAGGACAG CGGCTGAGCC CCGGTCCCCA 
GGGCCAGTTC CTCACCCGGC CCAGAGCAAG GCCACTGAGG CTGGGGGTGG AAACCCAAGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCTATTA TCGGAGTGAA AGAGAAGACA 
TTCGAGCAAC TTCACAAGAA ATGTCTAGAA AAGAAAGTTC TTTATGTGGA CCCTGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTATAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGCGA GAATCCCCGA TTTATCATTG ATGGAGCCAA CAGAACTGAC 
ATCTGTCAAG GAGAGCTAGG GGACTGCTGG TTTCTCGCAG CCATTGCCTG CCTGACCCTG 
AACCAGCACC TTCTTTTCCG AGTCATACCC CATGATCAAA GTTTCATCGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGAGTGGG TGGACGTGGT TATAGATGAC 
TGCCTGCCAA CGTACAACAA TCAACTGGTT TTCACCAAGT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCCTACGA AGCTCTGAAA 
GGTGGGAACA CCACAGAGGC CATGGAGGAC TTCACAGGAG GGGTGGCAGA GTTTTTTGAG 
ATCAGGGATG CTCCTAGTGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACG AACATGACCT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAGTTGAT TGCACGGATG GTAAGGAATA TGGATAACTC ACTGCTCCAG 
GACTCAGACC TCGACCCCAG AGGCTCAGAT GAAAGACCGA CCCGGACAAT CATTCCGGTT 
CAGTATGAGA CAAGAATGGC CTGCGGGCTG GTCAGAGGTC ACGCCTACTC TGTCACGGGG 
CTGGATGAGG TCCCGTTCAA AGGTGAGAAA GTGAAGCTGG TGCGGCTGCG GAATCCGTGG 
GGCCAGGTGG AGTGGAACGG TTCTTGGAGT GATAGATGGA AGGACTGGAG CTTTGTGGAC 
AAAGATGAGA AGGCCCGTCT GCAGCACCAG GTCACTGAGG ATGGAGAGTT CTGGATGTCC 
TATGAGGATT TCATCTACCA TTTCACAAAG TTGGAGATCT GCAACCTCAC GGCCGATGCT 
CTGCAGTCTG ACAAGCTTCA GACCTGGACA GTGTCTGTGA ACGAGGGCCG CTGGGTACGG 
GGTTGCTCTG CCGGAGGCTG CCGCAACTTC CCAGATACTT TCTGGACCAA CCCTCAGTAC 
CGTCTGAAGC TCCTGGAGGA GGACGATGAC CCTGATGACT CGGAGGTGAT TTGCAGCTTC 
CTGGTGGCCC TGATGCAGAA GAACCGGCGG AAGGACCGGA AGCTAGGGGC CAGTCTCTTC 
ACCATTGGCT TCGCCATCTA CGAGGTTCCC AAAGAGATGC ACGGGAACAA GCAGCACCTG 
CAGAAGGACT TCTTCCTGTA CAACGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCT CCCAGCGAGT ACGTCATCGT GCCCTCCACC 
TACGAGCCCC ACCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAACCTC 
TCTGAGGAAG TTGAAAATAC CATCTCCGTG GATCGGCCAG TGAAAAAGAA AAAAACCAAG 
CCCATCATCT TCGTTTCGGA CAGAGCAAAC AGCAACAAGG AGCTGGGTGT GGACCAGGAG 
TCAGAGGAGG GCAAAGGCAA AACAAGCCCT GATAAGCAAA AGCAGTCCCC ACAGCCACAG 
CCTGGCAGCT CTGATCAGGA AAGTGAGGAA CAGCAACAAT TCCGGAACAT TTTCAAGCAG 
ATAGCAGGAG ATGACATGGA GATCTGTGCA GATGAGCTCA AGAAGGTCCT TAACACAGTC 
GTGAACAAAC ACAAGGACCT GAAGACACAC GGGTTCACAC TGGAGTCCTG CCGTAGCATG 
ATTGCGCTCA TGGATACAGA TGGCTCTGGA AAGCTCAACC TGCAGGAGTT CCACCACCTC 
TGGAACAAGA TTAAGGCCTG GCAGAAAATT TTCAAACACT ATGACACAGA CCAGTCCGGC 
ACCATCAACA GCTACGAGAT GCGAAATGCA GTCAACGACG CAGGATTCCA CCTCAACAAC 
CAGCTCTATG ACATCATTAC CATGCGGTAC GCAGACAAAC ACATGAACAT CGACTTTGAC 
AGTTTCATCT GCTGCTTCGT TAGGCTGGAG GGCATGTTCA GAGCTTTTCA TGCATTTGAC 
AAGGATGGAG ATGGTATCAT CAAGCTCAAC GTTCTGGAGT GGCTGCAGCT CACCATGTAT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN3 ( NM_173087.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_775110.1 Homo sapiens calpain 3 (CAPN3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28275
Clone ID Related Accession (Same CDS sequence)	NM_173087.1
Accession Version	NM_173087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	calpain-3 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF127765.3 and AF209502.1. Transcript Variant: This variant (3) lacks three in-frame exons in the coding region, compared to variant 1. It encodes isoform c, which is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF127765.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGACCG TCATTAGCGC ATCTGTGGCT CCAAGGACAG CGGCTGAGCC CCGGTCCCCA 
GGGCCAGTTC CTCACCCGGC CCAGAGCAAG GCCACTGAGG CTGGGGGTGG AAACCCAAGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCTATTA TCGGAGTGAA AGAGAAGACA 
TTCGAGCAAC TTCACAAGAA ATGTCTAGAA AAGAAAGTTC TTTATGTGGA CCCTGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTATAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGCGA GAATCCCCGA TTTATCATTG ATGGAGCCAA CAGAACTGAC 
ATCTGTCAAG GAGAGCTAGG GGACTGCTGG TTTCTCGCAG CCATTGCCTG CCTGACCCTG 
AACCAGCACC TTCTTTTCCG AGTCATACCC CATGATCAAA GTTTCATCGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGAGTGGG TGGACGTGGT TATAGATGAC 
TGCCTGCCAA CGTACAACAA TCAACTGGTT TTCACCAAGT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCCTACGA AGCTCTGAAA 
GGTGGGAACA CCACAGAGGC CATGGAGGAC TTCACAGGAG GGGTGGCAGA GTTTTTTGAG 
ATCAGGGATG CTCCTAGTGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TACAATCATT CCGGTTCAGT ATGAGACAAG AATGGCCTGC 
GGGCTGGTCA GAGGTCACGC CTACTCTGTC ACGGGGCTGG ATGAGGTCCC GTTCAAAGGT 
GAGAAAGTGA AGCTGGTGCG GCTGCGGAAT CCGTGGGGCC AGGTGGAGTG GAACGGTTCT 
TGGAGTGATA GATGGAAGGA CTGGAGCTTT GTGGACAAAG ATGAGAAGGC CCGTCTGCAG 
CACCAGGTCA CTGAGGATGG AGAGTTCTGG ATGTCCTATG AGGATTTCAT CTACCATTTC 
ACAAAGTTGG AGATCTGCAA CCTCACGGCC GATGCTCTGC AGTCTGACAA GCTTCAGACC 
TGGACAGTGT CTGTGAACGA GGGCCGCTGG GTACGGGGTT GCTCTGCCGG AGGCTGCCGC 
AACTTCCCAG ATACTTTCTG GACCAACCCT CAGTACCGTC TGAAGCTCCT GGAGGAGGAC 
GATGACCCTG ATGACTCGGA GGTGATTTGC AGCTTCCTGG TGGCCCTGAT GCAGAAGAAC 
CGGCGGAAGG ACCGGAAGCT AGGGGCCAGT CTCTTCACCA TTGGCTTCGC CATCTACGAG 
GTTCCCAAAG AGATGCACGG GAACAAGCAG CACCTGCAGA AGGACTTCTT CCTGTACAAC 
GCCTCCAAGG CCAGGAGCAA AACCTACATC AACATGCGGG AGGTGTCCCA GCGCTTCCGC 
CTGCCTCCCA GCGAGTACGT CATCGTGCCC TCCACCTACG AGCCCCACCA GGAGGGGGAA 
TTCATCCTCC GGGTCTTCTC TGAAAAGAGG AACCTCTCTG AGGAAGTTGA AAATACCATC 
TCCGTGGATC GGCCAGTGCC ACAGCCTGGC AGCTCTGATC AGGAAAGTGA GGAACAGCAA 
CAATTCCGGA ACATTTTCAA GCAGATAGCA GGAGATGACA TGGAGATCTG TGCAGATGAG 
CTCAAGAAGG TCCTTAACAC AGTCGTGAAC AAACACAAGG ACCTGAAGAC ACACGGGTTC 
ACACTGGAGT CCTGCCGTAG CATGATTGCG CTCATGGATA CAGATGGCTC TGGAAAGCTC 
AACCTGCAGG AGTTCCACCA CCTCTGGAAC AAGATTAAGG CCTGGCAGAA AATTTTCAAA 
CACTATGACA CAGACCAGTC CGGCACCATC AACAGCTACG AGATGCGAAA TGCAGTCAAC 
GACGCAGGAT TCCACCTCAA CAACCAGCTC TATGACATCA TTACCATGCG GTACGCAGAC 
AAACACATGA ACATCGACTT TGACAGTTTC ATCTGCTGCT TCGTTAGGCT GGAGGGCATG 
TTCAGAGCTT TTCATGCATT TGACAAGGAT GGAGATGGTA TCATCAAGCT CAACGTTCTG 
GAGTGGCTGC AGCTCACCAT GTATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775110.1
CDS	307..2496
Translation	MPTVISASVAPRTAAEPRSPGPVPHPAQSKATEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVAEFFEIRDAPSDMYKIMKKAIERGSLMGCSIDTIIPVQYETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQVEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGASLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVPQPGSSDQESEEQQQFRNIFKQIAGDDMEICADELKKVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	NM_173087.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 3 (CAPN3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173087.1

ORF Insert Sequence:

ATGCCGACCG TCATTAGCGC ATCTGTGGCT CCAAGGACAG CGGCTGAGCC CCGGTCCCCA 
GGGCCAGTTC CTCACCCGGC CCAGAGCAAG GCCACTGAGG CTGGGGGTGG AAACCCAAGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCTATTA TCGGAGTGAA AGAGAAGACA 
TTCGAGCAAC TTCACAAGAA ATGTCTAGAA AAGAAAGTTC TTTATGTGGA CCCTGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTATAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGCGA GAATCCCCGA TTTATCATTG ATGGAGCCAA CAGAACTGAC 
ATCTGTCAAG GAGAGCTAGG GGACTGCTGG TTTCTCGCAG CCATTGCCTG CCTGACCCTG 
AACCAGCACC TTCTTTTCCG AGTCATACCC CATGATCAAA GTTTCATCGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGAGTGGG TGGACGTGGT TATAGATGAC 
TGCCTGCCAA CGTACAACAA TCAACTGGTT TTCACCAAGT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCCTACGA AGCTCTGAAA 
GGTGGGAACA CCACAGAGGC CATGGAGGAC TTCACAGGAG GGGTGGCAGA GTTTTTTGAG 
ATCAGGGATG CTCCTAGTGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TACAATCATT CCGGTTCAGT ATGAGACAAG AATGGCCTGC 
GGGCTGGTCA GAGGTCACGC CTACTCTGTC ACGGGGCTGG ATGAGGTCCC GTTCAAAGGT 
GAGAAAGTGA AGCTGGTGCG GCTGCGGAAT CCGTGGGGCC AGGTGGAGTG GAACGGTTCT 
TGGAGTGATA GATGGAAGGA CTGGAGCTTT GTGGACAAAG ATGAGAAGGC CCGTCTGCAG 
CACCAGGTCA CTGAGGATGG AGAGTTCTGG ATGTCCTATG AGGATTTCAT CTACCATTTC 
ACAAAGTTGG AGATCTGCAA CCTCACGGCC GATGCTCTGC AGTCTGACAA GCTTCAGACC 
TGGACAGTGT CTGTGAACGA GGGCCGCTGG GTACGGGGTT GCTCTGCCGG AGGCTGCCGC 
AACTTCCCAG ATACTTTCTG GACCAACCCT CAGTACCGTC TGAAGCTCCT GGAGGAGGAC 
GATGACCCTG ATGACTCGGA GGTGATTTGC AGCTTCCTGG TGGCCCTGAT GCAGAAGAAC 
CGGCGGAAGG ACCGGAAGCT AGGGGCCAGT CTCTTCACCA TTGGCTTCGC CATCTACGAG 
GTTCCCAAAG AGATGCACGG GAACAAGCAG CACCTGCAGA AGGACTTCTT CCTGTACAAC 
GCCTCCAAGG CCAGGAGCAA AACCTACATC AACATGCGGG AGGTGTCCCA GCGCTTCCGC 
CTGCCTCCCA GCGAGTACGT CATCGTGCCC TCCACCTACG AGCCCCACCA GGAGGGGGAA 
TTCATCCTCC GGGTCTTCTC TGAAAAGAGG AACCTCTCTG AGGAAGTTGA AAATACCATC 
TCCGTGGATC GGCCAGTGCC ACAGCCTGGC AGCTCTGATC AGGAAAGTGA GGAACAGCAA 
CAATTCCGGA ACATTTTCAA GCAGATAGCA GGAGATGACA TGGAGATCTG TGCAGATGAG 
CTCAAGAAGG TCCTTAACAC AGTCGTGAAC AAACACAAGG ACCTGAAGAC ACACGGGTTC 
ACACTGGAGT CCTGCCGTAG CATGATTGCG CTCATGGATA CAGATGGCTC TGGAAAGCTC 
AACCTGCAGG AGTTCCACCA CCTCTGGAAC AAGATTAAGG CCTGGCAGAA AATTTTCAAA 
CACTATGACA CAGACCAGTC CGGCACCATC AACAGCTACG AGATGCGAAA TGCAGTCAAC 
GACGCAGGAT TCCACCTCAA CAACCAGCTC TATGACATCA TTACCATGCG GTACGCAGAC 
AAACACATGA ACATCGACTT TGACAGTTTC ATCTGCTGCT TCGTTAGGCT GGAGGGCATG 
TTCAGAGCTT TTCATGCATT TGACAAGGAT GGAGATGGTA TCATCAAGCT CAACGTTCTG 
GAGTGGCTGC AGCTCACCAT GTATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN3 ( NM_173088.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_775111.1 Homo sapiens calpain 3 (CAPN3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu28277
Clone ID Related Accession (Same CDS sequence)	NM_173088.1
Accession Version	NM_173088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpain-3 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC003169.1. Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region, and uses a downstream start codon, compared to variant 1. It encodes isoform d, which has a shorter N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003169.1, SRR1803616.332973.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACGGGA ACAAGCAGCA CCTGCAGAAG GACTTCTTCC TGTACAACGC CTCCAAGGCC 
AGGAGCAAAA CCTACATCAA CATGCGGGAG GTGTCCCAGC GCTTCCGCCT GCCTCCCAGC 
GAGTACGTCA TCGTGCCCTC CACCTACGAG CCCCACCAGG AGGGGGAATT CATCCTCCGG 
GTCTTCTCTG AAAAGAGGAA CCTCTCTGAG GAAGTTGAAA ATACCATCTC CGTGGATCGG 
CCAGTGAAAA AGAAAAAAAC CAAGCCCATC ATCTTCGTTT CGGACAGAGC AAACAGCAAC 
AAGGAGCTGG GTGTGGACCA GGAGTCAGAG GAGGGCAAAG GCAAAACAAG CCCTGATAAG 
CAAAAGCAGT CCCCACAGCC ACAGCCTGGC AGCTCTGATC AGGAAAGTGA GGAACAGCAA 
CAATTCCGGA ACATTTTCAA GCAGATAGCA GGAGATGACA TGGAGATCTG TGCAGATGAG 
CTCAAGAAGG TCCTTAACAC AGTCGTGAAC AAACACAAGG ACCTGAAGAC ACACGGGTTC 
ACACTGGAGT CCTGCCGTAG CATGATTGCG CTCATGGATA CAGATGGCTC TGGAAAGCTC 
AACCTGCAGG AGTTCCACCA CCTCTGGAAC AAGATTAAGG CCTGGCAGAA AATTTTCAAA 
CACTATGACA CAGACCAGTC CGGCACCATC AACAGCTACG AGATGCGAAA TGCAGTCAAC 
GACGCAGGAT TCCACCTCAA CAACCAGCTC TATGACATCA TTACCATGCG GTACGCAGAC 
AAACACATGA ACATCGACTT TGACAGTTTC ATCTGCTGCT TCGTTAGGCT GGAGGGCATG 
TTCAGAGCTT TTCATGCATT TGACAAGGAT GGAGATGGTA TCATCAAGCT CAACGTTCTG 
GAGTGGCTGC AGCTCACCAT GTATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775111.1
CDS	170..1099
Translation	MHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVKKKKTKPIIFVSDRANSNKELGVDQESEEGKGKTSPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQIAGDDMEICADELKKVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	NM_173088.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 3 (CAPN3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173088.1

ORF Insert Sequence:

ATGCACGGGA ACAAGCAGCA CCTGCAGAAG GACTTCTTCC TGTACAACGC CTCCAAGGCC 
AGGAGCAAAA CCTACATCAA CATGCGGGAG GTGTCCCAGC GCTTCCGCCT GCCTCCCAGC 
GAGTACGTCA TCGTGCCCTC CACCTACGAG CCCCACCAGG AGGGGGAATT CATCCTCCGG 
GTCTTCTCTG AAAAGAGGAA CCTCTCTGAG GAAGTTGAAA ATACCATCTC CGTGGATCGG 
CCAGTGAAAA AGAAAAAAAC CAAGCCCATC ATCTTCGTTT CGGACAGAGC AAACAGCAAC 
AAGGAGCTGG GTGTGGACCA GGAGTCAGAG GAGGGCAAAG GCAAAACAAG CCCTGATAAG 
CAAAAGCAGT CCCCACAGCC ACAGCCTGGC AGCTCTGATC AGGAAAGTGA GGAACAGCAA 
CAATTCCGGA ACATTTTCAA GCAGATAGCA GGAGATGACA TGGAGATCTG TGCAGATGAG 
CTCAAGAAGG TCCTTAACAC AGTCGTGAAC AAACACAAGG ACCTGAAGAC ACACGGGTTC 
ACACTGGAGT CCTGCCGTAG CATGATTGCG CTCATGGATA CAGATGGCTC TGGAAAGCTC 
AACCTGCAGG AGTTCCACCA CCTCTGGAAC AAGATTAAGG CCTGGCAGAA AATTTTCAAA 
CACTATGACA CAGACCAGTC CGGCACCATC AACAGCTACG AGATGCGAAA TGCAGTCAAC 
GACGCAGGAT TCCACCTCAA CAACCAGCTC TATGACATCA TTACCATGCG GTACGCAGAC 
AAACACATGA ACATCGACTT TGACAGTTTC ATCTGCTGCT TCGTTAGGCT GGAGGGCATG 
TTCAGAGCTT TTCATGCATT TGACAAGGAT GGAGATGGTA TCATCAAGCT CAACGTTCTG 
GAGTGGCTGC AGCTCACCAT GTATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN3 ( NM_173089.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_775112.1 Homo sapiens calpain 3 (CAPN3), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu28285
Clone ID Related Accession (Same CDS sequence)	NM_173089.1 , NM_173090.1
Accession Version	NM_173089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpain-3 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC004883.2 and BC003169.1. Transcript Variant: This variant (5) differs in the 5' UTR and uses a downstream start codon, compared to variant 1. It encodes isoform e, which has a shorter N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC004883.2, ERR279838.2234.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGATCT GTGCAGATGA GCTCAAGAAG GTCCTTAACA CAGTCGTGAA CAAACACAAG 
GACCTGAAGA CACACGGGTT CACACTGGAG TCCTGCCGTA GCATGATTGC GCTCATGGAT 
ACAGATGGCT CTGGAAAGCT CAACCTGCAG GAGTTCCACC ACCTCTGGAA CAAGATTAAG 
GCCTGGCAGA AAATTTTCAA ACACTATGAC ACAGACCAGT CCGGCACCAT CAACAGCTAC 
GAGATGCGAA ATGCAGTCAA CGACGCAGGA TTCCACCTCA ACAACCAGCT CTATGACATC 
ATTACCATGC GGTACGCAGA CAAACACATG AACATCGACT TTGACAGTTT CATCTGCTGC 
TTCGTTAGGC TGGAGGGCAT GTTCAGAGCT TTTCATGCAT TTGACAAGGA TGGAGATGGT 
ATCATCAAGC TCAACGTTCT GGAGTGGCTG CAGCTCACCA TGTATGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775112.1
CDS	259..729
Translation	MEICADELKKVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	NM_173089.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 3 (CAPN3), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173089.1

ORF Insert Sequence:

ATGGAGATCT GTGCAGATGA GCTCAAGAAG GTCCTTAACA CAGTCGTGAA CAAACACAAG 
GACCTGAAGA CACACGGGTT CACACTGGAG TCCTGCCGTA GCATGATTGC GCTCATGGAT 
ACAGATGGCT CTGGAAAGCT CAACCTGCAG GAGTTCCACC ACCTCTGGAA CAAGATTAAG 
GCCTGGCAGA AAATTTTCAA ACACTATGAC ACAGACCAGT CCGGCACCAT CAACAGCTAC 
GAGATGCGAA ATGCAGTCAA CGACGCAGGA TTCCACCTCA ACAACCAGCT CTATGACATC 
ATTACCATGC GGTACGCAGA CAAACACATG AACATCGACT TTGACAGTTT CATCTGCTGC 
TTCGTTAGGC TGGAGGGCAT GTTCAGAGCT TTTCATGCAT TTGACAAGGA TGGAGATGGT 
ATCATCAAGC TCAACGTTCT GGAGTGGCTG CAGCTCACCA TGTATGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN3 ( NM_173090.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_775113.1 Homo sapiens calpain 3 (CAPN3), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu28285
Clone ID Related Accession (Same CDS sequence)	NM_173089.1 , NM_173090.1
Accession Version	NM_173090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpain-3 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC007810.2 and BC003169.1. Transcript Variant: This variant (6) differs in the 5' UTR and uses a downstream start codon, compared to variant 1. It encodes isoform e, which has a shorter N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007810.2, SRR1803612.324490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGATCT GTGCAGATGA GCTCAAGAAG GTCCTTAACA CAGTCGTGAA CAAACACAAG 
GACCTGAAGA CACACGGGTT CACACTGGAG TCCTGCCGTA GCATGATTGC GCTCATGGAT 
ACAGATGGCT CTGGAAAGCT CAACCTGCAG GAGTTCCACC ACCTCTGGAA CAAGATTAAG 
GCCTGGCAGA AAATTTTCAA ACACTATGAC ACAGACCAGT CCGGCACCAT CAACAGCTAC 
GAGATGCGAA ATGCAGTCAA CGACGCAGGA TTCCACCTCA ACAACCAGCT CTATGACATC 
ATTACCATGC GGTACGCAGA CAAACACATG AACATCGACT TTGACAGTTT CATCTGCTGC 
TTCGTTAGGC TGGAGGGCAT GTTCAGAGCT TTTCATGCAT TTGACAAGGA TGGAGATGGT 
ATCATCAAGC TCAACGTTCT GGAGTGGCTG CAGCTCACCA TGTATGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775113.1
CDS	277..747
Translation	MEICADELKKVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	NM_173090.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 3 (CAPN3), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173090.1

ORF Insert Sequence:

ATGGAGATCT GTGCAGATGA GCTCAAGAAG GTCCTTAACA CAGTCGTGAA CAAACACAAG 
GACCTGAAGA CACACGGGTT CACACTGGAG TCCTGCCGTA GCATGATTGC GCTCATGGAT 
ACAGATGGCT CTGGAAAGCT CAACCTGCAG GAGTTCCACC ACCTCTGGAA CAAGATTAAG 
GCCTGGCAGA AAATTTTCAA ACACTATGAC ACAGACCAGT CCGGCACCAT CAACAGCTAC 
GAGATGCGAA ATGCAGTCAA CGACGCAGGA TTCCACCTCA ACAACCAGCT CTATGACATC 
ATTACCATGC GGTACGCAGA CAAACACATG AACATCGACT TTGACAGTTT CATCTGCTGC 
TTCGTTAGGC TGGAGGGCAT GTTCAGAGCT TTTCATGCAT TTGACAAGGA TGGAGATGGT 
ATCATCAAGC TCAACGTTCT GGAGTGGCTG CAGCTCACCA TGTATGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN3 ( NM_024344.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_077320.1 Homo sapiens calpain 3 (CAPN3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28286
Clone ID Related Accession (Same CDS sequence)	NM_024344.1
Accession Version	NM_024344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	calpain-3 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF127764.1 and AF209502.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. It encodes isoform b, which is slightly shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF127764.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGACCG TCATTAGCGC ATCTGTGGCT CCAAGGACAG CGGCTGAGCC CCGGTCCCCA 
GGGCCAGTTC CTCACCCGGC CCAGAGCAAG GCCACTGAGG CTGGGGGTGG AAACCCAAGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCTATTA TCGGAGTGAA AGAGAAGACA 
TTCGAGCAAC TTCACAAGAA ATGTCTAGAA AAGAAAGTTC TTTATGTGGA CCCTGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTATAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGCGA GAATCCCCGA TTTATCATTG ATGGAGCCAA CAGAACTGAC 
ATCTGTCAAG GAGAGCTAGG GGACTGCTGG TTTCTCGCAG CCATTGCCTG CCTGACCCTG 
AACCAGCACC TTCTTTTCCG AGTCATACCC CATGATCAAA GTTTCATCGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGAGTGGG TGGACGTGGT TATAGATGAC 
TGCCTGCCAA CGTACAACAA TCAACTGGTT TTCACCAAGT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCCTACGA AGCTCTGAAA 
GGTGGGAACA CCACAGAGGC CATGGAGGAC TTCACAGGAG GGGTGGCAGA GTTTTTTGAG 
ATCAGGGATG CTCCTAGTGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACG AACATGACCT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAGTTGAT TGCACGGATG GTAAGGAATA TGGATAACTC ACTGCTCCAG 
GACTCAGACC TCGACCCCAG AGGCTCAGAT GAAAGACCGA CCCGGACAAT CATTCCGGTT 
CAGTATGAGA CAAGAATGGC CTGCGGGCTG GTCAGAGGTC ACGCCTACTC TGTCACGGGG 
CTGGATGAGG TCCCGTTCAA AGGTGAGAAA GTGAAGCTGG TGCGGCTGCG GAATCCGTGG 
GGCCAGGTGG AGTGGAACGG TTCTTGGAGT GATAGATGGA AGGACTGGAG CTTTGTGGAC 
AAAGATGAGA AGGCCCGTCT GCAGCACCAG GTCACTGAGG ATGGAGAGTT CTGGATGTCC 
TATGAGGATT TCATCTACCA TTTCACAAAG TTGGAGATCT GCAACCTCAC GGCCGATGCT 
CTGCAGTCTG ACAAGCTTCA GACCTGGACA GTGTCTGTGA ACGAGGGCCG CTGGGTACGG 
GGTTGCTCTG CCGGAGGCTG CCGCAACTTC CCAGATACTT TCTGGACCAA CCCTCAGTAC 
CGTCTGAAGC TCCTGGAGGA GGACGATGAC CCTGATGACT CGGAGGTGAT TTGCAGCTTC 
CTGGTGGCCC TGATGCAGAA GAACCGGCGG AAGGACCGGA AGCTAGGGGC CAGTCTCTTC 
ACCATTGGCT TCGCCATCTA CGAGGTTCCC AAAGAGATGC ACGGGAACAA GCAGCACCTG 
CAGAAGGACT TCTTCCTGTA CAACGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCT CCCAGCGAGT ACGTCATCGT GCCCTCCACC 
TACGAGCCCC ACCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAACCTC 
TCTGAGGAAG TTGAAAATAC CATCTCCGTG GATCGGCCAG TGCCCATCAT CTTCGTTTCG 
GACAGAGCAA ACAGCAACAA GGAGCTGGGT GTGGACCAGG AGTCAGAGGA GGGCAAAGGC 
AAAACAAGCC CTGATAAGCA AAAGCAGTCC CCACAGCCAC AGCCTGGCAG CTCTGATCAG 
GAAAGTGAGG AACAGCAACA ATTCCGGAAC ATTTTCAAGC AGATAGCAGG AGATGACATG 
GAGATCTGTG CAGATGAGCT CAAGAAGGTC CTTAACACAG TCGTGAACAA ACACAAGGAC 
CTGAAGACAC ACGGGTTCAC ACTGGAGTCC TGCCGTAGCA TGATTGCGCT CATGGATACA 
GATGGCTCTG GAAAGCTCAA CCTGCAGGAG TTCCACCACC TCTGGAACAA GATTAAGGCC 
TGGCAGAAAA TTTTCAAACA CTATGACACA GACCAGTCCG GCACCATCAA CAGCTACGAG 
ATGCGAAATG CAGTCAACGA CGCAGGATTC CACCTCAACA ACCAGCTCTA TGACATCATT 
ACCATGCGGT ACGCAGACAA ACACATGAAC ATCGACTTTG ACAGTTTCAT CTGCTGCTTC 
GTTAGGCTGG AGGGCATGTT CAGAGCTTTT CATGCATTTG ACAAGGATGG AGATGGTATC 
ATCAAGCTCA ACGTTCTGGA GTGGCTGCAG CTCACCATGT ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077320.1
CDS	307..2754
Translation	MPTVISASVAPRTAAEPRSPGPVPHPAQSKATEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVAEFFEIRDAPSDMYKIMKKAIERGSLMGCSIDDGTNMTYGTSPSGLNMGELIARMVRNMDNSLLQDSDLDPRGSDERPTRTIIPVQYETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQVEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGASLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVPIIFVSDRANSNKELGVDQESEEGKGKTSPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQIAGDDMEICADELKKVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	NM_024344.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 3 (CAPN3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024344.1

ORF Insert Sequence:

ATGCCGACCG TCATTAGCGC ATCTGTGGCT CCAAGGACAG CGGCTGAGCC CCGGTCCCCA 
GGGCCAGTTC CTCACCCGGC CCAGAGCAAG GCCACTGAGG CTGGGGGTGG AAACCCAAGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCTATTA TCGGAGTGAA AGAGAAGACA 
TTCGAGCAAC TTCACAAGAA ATGTCTAGAA AAGAAAGTTC TTTATGTGGA CCCTGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTATAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGCGA GAATCCCCGA TTTATCATTG ATGGAGCCAA CAGAACTGAC 
ATCTGTCAAG GAGAGCTAGG GGACTGCTGG TTTCTCGCAG CCATTGCCTG CCTGACCCTG 
AACCAGCACC TTCTTTTCCG AGTCATACCC CATGATCAAA GTTTCATCGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGAGTGGG TGGACGTGGT TATAGATGAC 
TGCCTGCCAA CGTACAACAA TCAACTGGTT TTCACCAAGT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCCTACGA AGCTCTGAAA 
GGTGGGAACA CCACAGAGGC CATGGAGGAC TTCACAGGAG GGGTGGCAGA GTTTTTTGAG 
ATCAGGGATG CTCCTAGTGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACG AACATGACCT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAGTTGAT TGCACGGATG GTAAGGAATA TGGATAACTC ACTGCTCCAG 
GACTCAGACC TCGACCCCAG AGGCTCAGAT GAAAGACCGA CCCGGACAAT CATTCCGGTT 
CAGTATGAGA CAAGAATGGC CTGCGGGCTG GTCAGAGGTC ACGCCTACTC TGTCACGGGG 
CTGGATGAGG TCCCGTTCAA AGGTGAGAAA GTGAAGCTGG TGCGGCTGCG GAATCCGTGG 
GGCCAGGTGG AGTGGAACGG TTCTTGGAGT GATAGATGGA AGGACTGGAG CTTTGTGGAC 
AAAGATGAGA AGGCCCGTCT GCAGCACCAG GTCACTGAGG ATGGAGAGTT CTGGATGTCC 
TATGAGGATT TCATCTACCA TTTCACAAAG TTGGAGATCT GCAACCTCAC GGCCGATGCT 
CTGCAGTCTG ACAAGCTTCA GACCTGGACA GTGTCTGTGA ACGAGGGCCG CTGGGTACGG 
GGTTGCTCTG CCGGAGGCTG CCGCAACTTC CCAGATACTT TCTGGACCAA CCCTCAGTAC 
CGTCTGAAGC TCCTGGAGGA GGACGATGAC CCTGATGACT CGGAGGTGAT TTGCAGCTTC 
CTGGTGGCCC TGATGCAGAA GAACCGGCGG AAGGACCGGA AGCTAGGGGC CAGTCTCTTC 
ACCATTGGCT TCGCCATCTA CGAGGTTCCC AAAGAGATGC ACGGGAACAA GCAGCACCTG 
CAGAAGGACT TCTTCCTGTA CAACGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCT CCCAGCGAGT ACGTCATCGT GCCCTCCACC 
TACGAGCCCC ACCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAACCTC 
TCTGAGGAAG TTGAAAATAC CATCTCCGTG GATCGGCCAG TGCCCATCAT CTTCGTTTCG 
GACAGAGCAA ACAGCAACAA GGAGCTGGGT GTGGACCAGG AGTCAGAGGA GGGCAAAGGC 
AAAACAAGCC CTGATAAGCA AAAGCAGTCC CCACAGCCAC AGCCTGGCAG CTCTGATCAG 
GAAAGTGAGG AACAGCAACA ATTCCGGAAC ATTTTCAAGC AGATAGCAGG AGATGACATG 
GAGATCTGTG CAGATGAGCT CAAGAAGGTC CTTAACACAG TCGTGAACAA ACACAAGGAC 
CTGAAGACAC ACGGGTTCAC ACTGGAGTCC TGCCGTAGCA TGATTGCGCT CATGGATACA 
GATGGCTCTG GAAAGCTCAA CCTGCAGGAG TTCCACCACC TCTGGAACAA GATTAAGGCC 
TGGCAGAAAA TTTTCAAACA CTATGACACA GACCAGTCCG GCACCATCAA CAGCTACGAG 
ATGCGAAATG CAGTCAACGA CGCAGGATTC CACCTCAACA ACCAGCTCTA TGACATCATT 
ACCATGCGGT ACGCAGACAA ACACATGAAC ATCGACTTTG ACAGTTTCAT CTGCTGCTTC 
GTTAGGCTGG AGGGCATGTT CAGAGCTTTT CATGCATTTG ACAAGGATGG AGATGGTATC 
ATCAAGCTCA ACGTTCTGGA GTGGCTGCAG CTCACCATGT ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Autosomal dominant calpainopathy due to heterozygous CAPN3 c.643_663del21.
Muscle & nerve(2017 Sep)
Martinez-Thompson JM,Niu Z,Tracy JA,Moore SA,Swenson A,Wieben ED,Milone M

Integrative variants, haplotypes and diplotypes of the CAPN3 and FRMD5 genes and several environmental exposures associate with serum lipid variables.
Scientific reports745119(2017 Mar)
Guo T,Yin RX,Pan L,Yang S,Miao L,Huang F

Epidemiological and Molecular Characterization of a Mexican Population Isolate with High Prevalence of Limb-Girdle Muscular Dystrophy Type 2A Due to a Novel Calpain-3 Mutation.
PloS one12(1)e0170280(2017)
Pantoja-Melendez CA,Miranda-Duarte A,Roque-Ramirez B,Zenteno JC

Novel Homozygous Missense Mutation in CAPN3 Gene Detected in a Saudi Arabian Family With Limb-Girdle Muscular Dystrophy Type 2A.
Journal of clinical neuromuscular disease18(2)89-91(2016 Dec)
Al-Harbi TM,Abdulmana? SO,Dridi W

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CAPN3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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