HECTD3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECTD3
Entrez Gene ID	79654
Full Name	HECT domain E3 ubiquitin protein ligase 3
General protein information	Preferred Names HECT domain E3 ubiquitin protein ligase 3 Names E3 ubiquitin-protein ligase HECTD3 HECT domain containing E3 ubiquitin protein ligase 3 HECT-type E3 ubiquitin transferase HECTD3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.1
Summary	The protein encoded by this gene transfers ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, leading to the degradation of those substrates. The encoded protein has been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle progression, and to STX8. [provided by RefSeq, Dec 2012].

mRNA and Protein(s)

mRNA	Protein	Name
NM_024602.5	NP_078878.3	E3 ubiquitin-protein ligase HECTD3
NM_024602.6	NP_078878.3	E3 ubiquitin-protein ligase HECTD3
XM_024449803.1	XP_024305571.1	E3 ubiquitin-protein ligase HECTD3 isoform X1
XM_011542140.2	XP_011540442.1	E3 ubiquitin-protein ligase HECTD3 isoform X1

Pathways from BioSystems

Homologies

	HECTD3	XP_001100268.1
Canis lupus familiaris (dog)	HECTD3	XP_539635.2
Bos taurus (cattle)	HECTD3	NP_001092431.1
Mus musculus (house mouse)	Hectd3	NP_780453.1
Gallus gallus (chicken)	HECTD3	XP_422429.2
Danio rerio (zebrafish)	hectd3	NP_001070627.2
Xenopus tropicalis (tropical clawed frog)	hectd3	NP_001072497.1
Homo sapiens (human)	HECTD3	NP_078878.3
Pan troglodytes (chimpanzee)	HECTD3	XP_513128.4
Rattus norvegicus (Norway rat)	Hectd3	XP_003750059.1

Gene Ontology
Bibliography

Related articles in PubMed

HECTD3 Mediates an HSP90-Dependent Degradation Pathway for Protein Kinase Clients.
Li Z, Zhou L, Prodromou C, Savic V, Pearl LH
Cell reports19(12)2515-2528(2017 Jun)

The HECTD3 E3 ubiquitin ligase suppresses cisplatin-induced apoptosis via stabilizing MALT1.
Li Y, Chen X, Wang Z, Zhao D, Chen H, Chen W, Zhou Z, Zhang J, Zhang J, Li H, Chen C
Neoplasia (New York, N.Y.)15(1)39-48(2013 Jan)

The E3 ubiquitin ligase HECTD3 regulates ubiquitination and degradation of Tara.
Yu J, Lan J, Zhu Y, Li X, Lai X, Xue Y, Jin C, Huang H
Biochemical and biophysical research communications367(4)805-12(2008 Mar)

Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.
Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J
Nature biotechnology21(5)566-9(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of MALT1 partially rescues HECTD3 depletion-induced apoptosis.
Title: The HECTD3 E3 ubiquitin ligase suppresses cisplatin-induced apoptosis via stabilizing MALT1.

All these findings suggest that HECTD3 may facilitate cell cycle progression via regulating ubiquitination and degradation of Tara.
Title: The E3 ubiquitin ligase HECTD3 regulates ubiquitination and degradation of Tara.

The following HECTD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19706	NM_024602.6 Latest version!	Homo sapiens HECT domain E3 ubiquitin protein ligase 3 (HECTD3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$434.50-$608.30 $869.00
OHu71973	XM_011542140.2 Latest version!	Homo sapiens HECT domain E3 ubiquitin protein ligase 3 (HECTD3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu71973	XM_024449803.1 Latest version!	Homo sapiens HECT domain E3 ubiquitin protein ligase 3 (HECTD3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
View Old Accession Versions >>
OHu19706	NM_024602.5	Homo sapiens HECT domain E3 ubiquitin protein ligase 3 (HECTD3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$434.50-$608.30 $869.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECTD3 ( NM_024602.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_078878.3 Homo sapiens HECT domain E3 ubiquitin protein ligase 3 (HECTD3), mRNA.

Price & Availability↑

CloneID	OHu19706
Clone ID Related Accession (Same CDS sequence)	NM_024602.5 , NM_024602.6
Accession Version	NM_024602.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECTD3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA332198.1, AK096462.1, AK056545.1, AK056873.1 and AL359473.22. This sequence is a reference standard in the RefSeqGene project. On Sep 28, 2007 this sequence version replaced NM_024602.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTC CTGGCCCGGG CGCGGTGCTG GAGTCCCCCC GGCAGCTGCT GGGCCGCGTG 
CGCTTCTTGG CAGAGGCAGC GCGGAGCCTC CGCGCCGGGC GGCCGCTGCC AGCAGCGCTG 
GCTTTCGTGC CGCGAGAGGT GCTCTACAAG CTTTACAAGG ACCCAGCGGG ACCGTCGCGC 
GTGCTTCTGC CGGTGTGGGA GGCAGAGGGC CTGGGGCTGC GTGTGGGCGC CGCAGGCCCA 
GCCCCCGGTA CCGGCTCCGG GCCCCTCCGC GCCGCCCGCG ACAGCATTGA GCTCCGGCGC 
GGCGCCTGCG TGCGCACCAC GGGCGAGGAG CTGTGCAATG GCCACGGGCT CTGGGTGAAG 
CTGACAAAGG AGCAGCTGGC AGAGCACCTG GGCGACTGCG GGCTGCAGGA AGGCTGGCTG 
CTGGTGTGCC GCCCGGCGGA GGGCGGAGCC CGCCTGGTAC CCATCGACAC TCCCAACCAC 
CTCCAGCGGC AGCAGCAGCT CTTTGGCGTG GATTATCGGC CGGTGCTCAG GTGGGAACAG 
GTGGTGGACC TGACATACTC ACATCGCCTG GGATCGAGAC CTCAGCCGGC AGAGGCATAC 
GCAGAAGCTG TACAAAGGCT ACTCTATGTA CCCCCGACAT GGACCTACGA GTGCGACGAG 
GACCTGATCC ACTTCTTGTA TGACCACCTG GGCAAGGAGG ATGAGAACCT GGGTAGCGTG 
AAGCAGTATG TGGAGAGCAT AGACGTTTCC TCCTACACGG AGGAGTTCAA CGTGTCCTGC 
CTGACAGACA GCAATGCCGA TACCTACTGG GAGAGCGATG GGTCCCAGTG CCAACACTGG 
GTACGGCTTA CTATGAAGAA GGGCACCATT GTCAAGAAGC TGCTACTCAC AGTGGATACC 
ACAGATGACA ACTTTATGCC AAAGCGGGTG GTGGTCTATG GGGGTGAAGG GGACAACCTG 
AAGAAGCTGA GTGACGTGAG CATTGACGAG ACCCTCATCG GGGATGTCTG TGTCCTGGAG 
GACATGACCG TCCACCTCCC GATCATCGAG ATCCGCATCG TGGAGTGCCG AGATGATGGG 
ATTGATGTTC GTCTCCGAGG GGTCAAGATC AAGTCATCTA GACAGCGGGA ACTAGGGTTG 
AATGCAGACC TGTTCCAGCC AACTAGTCTG GTGCGATATC CACGCCTAGA AGGCACCGAC 
CCTGAAGTAC TGTACCGCAG AGCTGTCCTC CTGCAGAGAT TCATCAAGAT CCTCGATAGT 
GTCCTGCACC ACCTGGTACC TGCCTGGGAC CACACACTGG GCACCTTCAG TGAGATTAAG 
CAAGTGAAGC AGTTCCTACT GCTGTCCCGC CAGCGGCCAG GCCTGGTGGC TCAGTGCCTG 
CGTGACTCTG AGAGCAGCAA GCCCAGCTTC ATGCCACGCC TATACATCAA CCGCCGTCTT 
GCCATGGAAC ACCGTGCCTG CCCCTCTCGA GACCCTGCCT GCAAGAATGC AGTCTTCACC 
CAGGTATATG AAGGCCTCAA GCCCTCTGAC AAATATGAAA AGCCCCTGGA CTACAGGTGG 
CCCATGCGCT ATGACCAGTG GTGGGAGTGT AAATTTATTG CAGAAGGCAT CATTGACCAA 
GGGGGTGGTT TCCGGGACAG CCTGGCAGAT ATGTCAGAAG AGCTGTGCCC TAGCTCAGCG 
GATACCCCCG TGCCCCTGCC CTTCTTTGTA CGCACAGCCA ACCAGGGCAA TGGCACTGGT 
GAGGCTCGGG ACATGTATGT ACCCAACCCC TCCTGCCGAG ACTTTGCCAA GTATGAATGG 
ATCGGACAGC TGATGGGGGC TGCCCTTCGG GGTAAGGAGT TCCTGGTCCT GGCCCTGCCT 
GGTTTTGTGT GGAAGCAGCT TTCTGGTGAG GAGGTGAGCT GGAGCAAGGA CTTCCCAGCT 
GTGGACTCTG TGCTGGTGAA GCTCCTGGAA GTGATGGAAG GAATGGACAA GGAGACGTTT 
GAGTTCAAGT TTGGGAAGGA ACTAACATTC ACCACTGTAC TGAGTGACCA ACAGGTGGTG 
GAGCTGATCC CTGGGGGTGC AGGCATCGTC GTGGGATATG GGGACCGTTC TCGTTTCATC 
CAACTGGTCC AGAAGGCACG GCTAGAGGAG AGCAAGGAGC AGGTGGCAGC TATGCAGGCA 
GGTCTGCTGA AGGTGGTACC ACAGGCTGTG CTGGACTTGC TGACCTGGCA AGAGTTGGAG 
AAGAAAGTGT GTGGGGATCC AGAGGTCACT GTGGATGCTC TGCGCAAGCT CACCCGGTTT 
GAGGACTTCG AGCCATCTGA CTCGCGGGTG CAGTATTTCT GGGAGGCACT GAACAACTTC 
ACCAACGAGG ACCGGAGCCG CTTCCTGCGC TTTGTCACGG GCCGCAGTCG CCTGCCAGCA 
CGGATCTACA TCTACCCAGA CAAGCTGGGC TACGAGACCA CAGACGCGCT GCCCGAGTCT 
TCCACTTGCT CCAGCACCCT CTTCCTGCCA CACTATGCCA GTGCCAAGGT ATGCGAGGAG 
AAGCTCCGCT ATGCGGCCTA CAACTGCGTG GCCATCGACA CTGACATGAG CCCTTGGGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078878.3
CDS	99..2684
Translation	MAGPGPGAVLESPRQLLGRVRFLAEAARSLRAGRPLPAALAFVPREVLYKLYKDPAGPSRVLLPVWEAEGLGLRVGAAGPAPGTGSGPLRAARDSIELRRGACVRTTGEELCNGHGLWVKLTKEQLAEHLGDCGLQEGWLLVCRPAEGGARLVPIDTPNHLQRQQQLFGVDYRPVLRWEQVVDLTYSHRLGSRPQPAEAYAEAVQRLLYVPPTWTYECDEDLIHFLYDHLGKEDENLGSVKQYVESIDVSSYTEEFNVSCLTDSNADTYWESDGSQCQHWVRLTMKKGTIVKKLLLTVDTTDDNFMPKRVVVYGGEGDNLKKLSDVSIDETLIGDVCVLEDMTVHLPIIEIRIVECRDDGIDVRLRGVKIKSSRQRELGLNADLFQPTSLVRYPRLEGTDPEVLYRRAVLLQRFIKILDSVLHHLVPAWDHTLGTFSEIKQVKQFLLLSRQRPGLVAQCLRDSESSKPSFMPRLYINRRLAMEHRACPSRDPACKNAVFTQVYEGLKPSDKYEKPLDYRWPMRYDQWWECKFIAEGIIDQGGGFRDSLADMSEELCPSSADTPVPLPFFVRTANQGNGTGEARDMYVPNPSCRDFAKYEWIGQLMGAALRGKEFLVLALPGFVWKQLSGEEVSWSKDFPAVDSVLVKLLEVMEGMDKETFEFKFGKELTFTTVLSDQQVVELIPGGAGIVVGYGDRSRFIQLVQKARLEESKEQVAAMQAGLLKVVPQAVLDLLTWQELEKKVCGDPEVTVDALRKLTRFEDFEPSDSRVQYFWEALNNFTNEDRSRFLRFVTGRSRLPARIYIYPDKLGYETTDALPESSTCSSTLFLPHYASAKVCEEKLRYAAYNCVAIDTDMSPWEE

Target ORF information:

RefSeq Version	NM_024602.5
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain E3 ubiquitin protein ligase 3 (HECTD3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024602.5

ORF Insert Sequence:

ATGGCGGGTC CTGGCCCGGG CGCGGTGCTG GAGTCCCCCC GGCAGCTGCT GGGCCGCGTG 
CGCTTCTTGG CAGAGGCAGC GCGGAGCCTC CGCGCCGGGC GGCCGCTGCC AGCAGCGCTG 
GCTTTCGTGC CGCGAGAGGT GCTCTACAAG CTTTACAAGG ACCCAGCGGG ACCGTCGCGC 
GTGCTTCTGC CGGTGTGGGA GGCAGAGGGC CTGGGGCTGC GTGTGGGCGC CGCAGGCCCA 
GCCCCCGGTA CCGGCTCCGG GCCCCTCCGC GCCGCCCGCG ACAGCATTGA GCTCCGGCGC 
GGCGCCTGCG TGCGCACCAC GGGCGAGGAG CTGTGCAATG GCCACGGGCT CTGGGTGAAG 
CTGACAAAGG AGCAGCTGGC AGAGCACCTG GGCGACTGCG GGCTGCAGGA AGGCTGGCTG 
CTGGTGTGCC GCCCGGCGGA GGGCGGAGCC CGCCTGGTAC CCATCGACAC TCCCAACCAC 
CTCCAGCGGC AGCAGCAGCT CTTTGGCGTG GATTATCGGC CGGTGCTCAG GTGGGAACAG 
GTGGTGGACC TGACATACTC ACATCGCCTG GGATCGAGAC CTCAGCCGGC AGAGGCATAC 
GCAGAAGCTG TACAAAGGCT ACTCTATGTA CCCCCGACAT GGACCTACGA GTGCGACGAG 
GACCTGATCC ACTTCTTGTA TGACCACCTG GGCAAGGAGG ATGAGAACCT GGGTAGCGTG 
AAGCAGTATG TGGAGAGCAT AGACGTTTCC TCCTACACGG AGGAGTTCAA CGTGTCCTGC 
CTGACAGACA GCAATGCCGA TACCTACTGG GAGAGCGATG GGTCCCAGTG CCAACACTGG 
GTACGGCTTA CTATGAAGAA GGGCACCATT GTCAAGAAGC TGCTACTCAC AGTGGATACC 
ACAGATGACA ACTTTATGCC AAAGCGGGTG GTGGTCTATG GGGGTGAAGG GGACAACCTG 
AAGAAGCTGA GTGACGTGAG CATTGACGAG ACCCTCATCG GGGATGTCTG TGTCCTGGAG 
GACATGACCG TCCACCTCCC GATCATCGAG ATCCGCATCG TGGAGTGCCG AGATGATGGG 
ATTGATGTTC GTCTCCGAGG GGTCAAGATC AAGTCATCTA GACAGCGGGA ACTAGGGTTG 
AATGCAGACC TGTTCCAGCC AACTAGTCTG GTGCGATATC CACGCCTAGA AGGCACCGAC 
CCTGAAGTAC TGTACCGCAG AGCTGTCCTC CTGCAGAGAT TCATCAAGAT CCTCGATAGT 
GTCCTGCACC ACCTGGTACC TGCCTGGGAC CACACACTGG GCACCTTCAG TGAGATTAAG 
CAAGTGAAGC AGTTCCTACT GCTGTCCCGC CAGCGGCCAG GCCTGGTGGC TCAGTGCCTG 
CGTGACTCTG AGAGCAGCAA GCCCAGCTTC ATGCCACGCC TATACATCAA CCGCCGTCTT 
GCCATGGAAC ACCGTGCCTG CCCCTCTCGA GACCCTGCCT GCAAGAATGC AGTCTTCACC 
CAGGTATATG AAGGCCTCAA GCCCTCTGAC AAATATGAAA AGCCCCTGGA CTACAGGTGG 
CCCATGCGCT ATGACCAGTG GTGGGAGTGT AAATTTATTG CAGAAGGCAT CATTGACCAA 
GGGGGTGGTT TCCGGGACAG CCTGGCAGAT ATGTCAGAAG AGCTGTGCCC TAGCTCAGCG 
GATACCCCCG TGCCCCTGCC CTTCTTTGTA CGCACAGCCA ACCAGGGCAA TGGCACTGGT 
GAGGCTCGGG ACATGTATGT ACCCAACCCC TCCTGCCGAG ACTTTGCCAA GTATGAATGG 
ATCGGACAGC TGATGGGGGC TGCCCTTCGG GGTAAGGAGT TCCTGGTCCT GGCCCTGCCT 
GGTTTTGTGT GGAAGCAGCT TTCTGGTGAG GAGGTGAGCT GGAGCAAGGA CTTCCCAGCT 
GTGGACTCTG TGCTGGTGAA GCTCCTGGAA GTGATGGAAG GAATGGACAA GGAGACGTTT 
GAGTTCAAGT TTGGGAAGGA ACTAACATTC ACCACTGTAC TGAGTGACCA ACAGGTGGTG 
GAGCTGATCC CTGGGGGTGC AGGCATCGTC GTGGGATATG GGGACCGTTC TCGTTTCATC 
CAACTGGTCC AGAAGGCACG GCTAGAGGAG AGCAAGGAGC AGGTGGCAGC TATGCAGGCA 
GGTCTGCTGA AGGTGGTACC ACAGGCTGTG CTGGACTTGC TGACCTGGCA AGAGTTGGAG 
AAGAAAGTGT GTGGGGATCC AGAGGTCACT GTGGATGCTC TGCGCAAGCT CACCCGGTTT 
GAGGACTTCG AGCCATCTGA CTCGCGGGTG CAGTATTTCT GGGAGGCACT GAACAACTTC 
ACCAACGAGG ACCGGAGCCG CTTCCTGCGC TTTGTCACGG GCCGCAGTCG CCTGCCAGCA 
CGGATCTACA TCTACCCAGA CAAGCTGGGC TACGAGACCA CAGACGCGCT GCCCGAGTCT 
TCCACTTGCT CCAGCACCCT CTTCCTGCCA CACTATGCCA GTGCCAAGGT ATGCGAGGAG 
AAGCTCCGCT ATGCGGCCTA CAACTGCGTG GCCATCGACA CTGACATGAG CCCTTGGGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD3 ( NM_024602.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_078878.3 Homo sapiens HECT domain E3 ubiquitin protein ligase 3 (HECTD3), mRNA.

Price & Availability↑

CloneID	OHu19706
Clone ID Related Accession (Same CDS sequence)	NM_024602.5 , NM_024602.6
Accession Version	NM_024602.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECTD3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA332198.1, AK096462.1, AK056545.1, AK056873.1 and AL359473.22. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_024602.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.256753.1, SRR1660807.110935.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000372172.5/ ENSP00000361245.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGGTC CTGGCCCGGG CGCGGTGCTG GAGTCCCCCC GGCAGCTGCT GGGCCGCGTG 
CGCTTCTTGG CAGAGGCAGC GCGGAGCCTC CGCGCCGGGC GGCCGCTGCC AGCAGCGCTG 
GCTTTCGTGC CGCGAGAGGT GCTCTACAAG CTTTACAAGG ACCCAGCGGG ACCGTCGCGC 
GTGCTTCTGC CGGTGTGGGA GGCAGAGGGC CTGGGGCTGC GTGTGGGCGC CGCAGGCCCA 
GCCCCCGGTA CCGGCTCCGG GCCCCTCCGC GCCGCCCGCG ACAGCATTGA GCTCCGGCGC 
GGCGCCTGCG TGCGCACCAC GGGCGAGGAG CTGTGCAATG GCCACGGGCT CTGGGTGAAG 
CTGACAAAGG AGCAGCTGGC AGAGCACCTG GGCGACTGCG GGCTGCAGGA AGGCTGGCTG 
CTGGTGTGCC GCCCGGCGGA GGGCGGAGCC CGCCTGGTAC CCATCGACAC TCCCAACCAC 
CTCCAGCGGC AGCAGCAGCT CTTTGGCGTG GATTATCGGC CGGTGCTCAG GTGGGAACAG 
GTGGTGGACC TGACATACTC ACATCGCCTG GGATCGAGAC CTCAGCCGGC AGAGGCATAC 
GCAGAAGCTG TACAAAGGCT ACTCTATGTA CCCCCGACAT GGACCTACGA GTGCGACGAG 
GACCTGATCC ACTTCTTGTA TGACCACCTG GGCAAGGAGG ATGAGAACCT GGGTAGCGTG 
AAGCAGTATG TGGAGAGCAT AGACGTTTCC TCCTACACGG AGGAGTTCAA CGTGTCCTGC 
CTGACAGACA GCAATGCCGA TACCTACTGG GAGAGCGATG GGTCCCAGTG CCAACACTGG 
GTACGGCTTA CTATGAAGAA GGGCACCATT GTCAAGAAGC TGCTACTCAC AGTGGATACC 
ACAGATGACA ACTTTATGCC AAAGCGGGTG GTGGTCTATG GGGGTGAAGG GGACAACCTG 
AAGAAGCTGA GTGACGTGAG CATTGACGAG ACCCTCATCG GGGATGTCTG TGTCCTGGAG 
GACATGACCG TCCACCTCCC GATCATCGAG ATCCGCATCG TGGAGTGCCG AGATGATGGG 
ATTGATGTTC GTCTCCGAGG GGTCAAGATC AAGTCATCTA GACAGCGGGA ACTAGGGTTG 
AATGCAGACC TGTTCCAGCC AACTAGTCTG GTGCGATATC CACGCCTAGA AGGCACCGAC 
CCTGAAGTAC TGTACCGCAG AGCTGTCCTC CTGCAGAGAT TCATCAAGAT CCTCGATAGT 
GTCCTGCACC ACCTGGTACC TGCCTGGGAC CACACACTGG GCACCTTCAG TGAGATTAAG 
CAAGTGAAGC AGTTCCTACT GCTGTCCCGC CAGCGGCCAG GCCTGGTGGC TCAGTGCCTG 
CGTGACTCTG AGAGCAGCAA GCCCAGCTTC ATGCCACGCC TATACATCAA CCGCCGTCTT 
GCCATGGAAC ACCGTGCCTG CCCCTCTCGA GACCCTGCCT GCAAGAATGC AGTCTTCACC 
CAGGTATATG AAGGCCTCAA GCCCTCTGAC AAATATGAAA AGCCCCTGGA CTACAGGTGG 
CCCATGCGCT ATGACCAGTG GTGGGAGTGT AAATTTATTG CAGAAGGCAT CATTGACCAA 
GGGGGTGGTT TCCGGGACAG CCTGGCAGAT ATGTCAGAAG AGCTGTGCCC TAGCTCAGCG 
GATACCCCCG TGCCCCTGCC CTTCTTTGTA CGCACAGCCA ACCAGGGCAA TGGCACTGGT 
GAGGCTCGGG ACATGTATGT ACCCAACCCC TCCTGCCGAG ACTTTGCCAA GTATGAATGG 
ATCGGACAGC TGATGGGGGC TGCCCTTCGG GGTAAGGAGT TCCTGGTCCT GGCCCTGCCT 
GGTTTTGTGT GGAAGCAGCT TTCTGGTGAG GAGGTGAGCT GGAGCAAGGA CTTCCCAGCT 
GTGGACTCTG TGCTGGTGAA GCTCCTGGAA GTGATGGAAG GAATGGACAA GGAGACGTTT 
GAGTTCAAGT TTGGGAAGGA ACTAACATTC ACCACTGTAC TGAGTGACCA ACAGGTGGTG 
GAGCTGATCC CTGGGGGTGC AGGCATCGTC GTGGGATATG GGGACCGTTC TCGTTTCATC 
CAACTGGTCC AGAAGGCACG GCTAGAGGAG AGCAAGGAGC AGGTGGCAGC TATGCAGGCA 
GGTCTGCTGA AGGTGGTACC ACAGGCTGTG CTGGACTTGC TGACCTGGCA AGAGTTGGAG 
AAGAAAGTGT GTGGGGATCC AGAGGTCACT GTGGATGCTC TGCGCAAGCT CACCCGGTTT 
GAGGACTTCG AGCCATCTGA CTCGCGGGTG CAGTATTTCT GGGAGGCACT GAACAACTTC 
ACCAACGAGG ACCGGAGCCG CTTCCTGCGC TTTGTCACGG GCCGCAGTCG CCTGCCAGCA 
CGGATCTACA TCTACCCAGA CAAGCTGGGC TACGAGACCA CAGACGCGCT GCCCGAGTCT 
TCCACTTGCT CCAGCACCCT CTTCCTGCCA CACTATGCCA GTGCCAAGGT ATGCGAGGAG 
AAGCTCCGCT ATGCGGCCTA CAACTGCGTG GCCATCGACA CTGACATGAG CCCTTGGGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078878.3
CDS	68..2653
Translation	MAGPGPGAVLESPRQLLGRVRFLAEAARSLRAGRPLPAALAFVPREVLYKLYKDPAGPSRVLLPVWEAEGLGLRVGAAGPAPGTGSGPLRAARDSIELRRGACVRTTGEELCNGHGLWVKLTKEQLAEHLGDCGLQEGWLLVCRPAEGGARLVPIDTPNHLQRQQQLFGVDYRPVLRWEQVVDLTYSHRLGSRPQPAEAYAEAVQRLLYVPPTWTYECDEDLIHFLYDHLGKEDENLGSVKQYVESIDVSSYTEEFNVSCLTDSNADTYWESDGSQCQHWVRLTMKKGTIVKKLLLTVDTTDDNFMPKRVVVYGGEGDNLKKLSDVSIDETLIGDVCVLEDMTVHLPIIEIRIVECRDDGIDVRLRGVKIKSSRQRELGLNADLFQPTSLVRYPRLEGTDPEVLYRRAVLLQRFIKILDSVLHHLVPAWDHTLGTFSEIKQVKQFLLLSRQRPGLVAQCLRDSESSKPSFMPRLYINRRLAMEHRACPSRDPACKNAVFTQVYEGLKPSDKYEKPLDYRWPMRYDQWWECKFIAEGIIDQGGGFRDSLADMSEELCPSSADTPVPLPFFVRTANQGNGTGEARDMYVPNPSCRDFAKYEWIGQLMGAALRGKEFLVLALPGFVWKQLSGEEVSWSKDFPAVDSVLVKLLEVMEGMDKETFEFKFGKELTFTTVLSDQQVVELIPGGAGIVVGYGDRSRFIQLVQKARLEESKEQVAAMQAGLLKVVPQAVLDLLTWQELEKKVCGDPEVTVDALRKLTRFEDFEPSDSRVQYFWEALNNFTNEDRSRFLRFVTGRSRLPARIYIYPDKLGYETTDALPESSTCSSTLFLPHYASAKVCEEKLRYAAYNCVAIDTDMSPWEE

Target ORF information:

RefSeq Version	NM_024602.6
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain E3 ubiquitin protein ligase 3 (HECTD3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024602.6

ORF Insert Sequence:

ATGGCGGGTC CTGGCCCGGG CGCGGTGCTG GAGTCCCCCC GGCAGCTGCT GGGCCGCGTG 
CGCTTCTTGG CAGAGGCAGC GCGGAGCCTC CGCGCCGGGC GGCCGCTGCC AGCAGCGCTG 
GCTTTCGTGC CGCGAGAGGT GCTCTACAAG CTTTACAAGG ACCCAGCGGG ACCGTCGCGC 
GTGCTTCTGC CGGTGTGGGA GGCAGAGGGC CTGGGGCTGC GTGTGGGCGC CGCAGGCCCA 
GCCCCCGGTA CCGGCTCCGG GCCCCTCCGC GCCGCCCGCG ACAGCATTGA GCTCCGGCGC 
GGCGCCTGCG TGCGCACCAC GGGCGAGGAG CTGTGCAATG GCCACGGGCT CTGGGTGAAG 
CTGACAAAGG AGCAGCTGGC AGAGCACCTG GGCGACTGCG GGCTGCAGGA AGGCTGGCTG 
CTGGTGTGCC GCCCGGCGGA GGGCGGAGCC CGCCTGGTAC CCATCGACAC TCCCAACCAC 
CTCCAGCGGC AGCAGCAGCT CTTTGGCGTG GATTATCGGC CGGTGCTCAG GTGGGAACAG 
GTGGTGGACC TGACATACTC ACATCGCCTG GGATCGAGAC CTCAGCCGGC AGAGGCATAC 
GCAGAAGCTG TACAAAGGCT ACTCTATGTA CCCCCGACAT GGACCTACGA GTGCGACGAG 
GACCTGATCC ACTTCTTGTA TGACCACCTG GGCAAGGAGG ATGAGAACCT GGGTAGCGTG 
AAGCAGTATG TGGAGAGCAT AGACGTTTCC TCCTACACGG AGGAGTTCAA CGTGTCCTGC 
CTGACAGACA GCAATGCCGA TACCTACTGG GAGAGCGATG GGTCCCAGTG CCAACACTGG 
GTACGGCTTA CTATGAAGAA GGGCACCATT GTCAAGAAGC TGCTACTCAC AGTGGATACC 
ACAGATGACA ACTTTATGCC AAAGCGGGTG GTGGTCTATG GGGGTGAAGG GGACAACCTG 
AAGAAGCTGA GTGACGTGAG CATTGACGAG ACCCTCATCG GGGATGTCTG TGTCCTGGAG 
GACATGACCG TCCACCTCCC GATCATCGAG ATCCGCATCG TGGAGTGCCG AGATGATGGG 
ATTGATGTTC GTCTCCGAGG GGTCAAGATC AAGTCATCTA GACAGCGGGA ACTAGGGTTG 
AATGCAGACC TGTTCCAGCC AACTAGTCTG GTGCGATATC CACGCCTAGA AGGCACCGAC 
CCTGAAGTAC TGTACCGCAG AGCTGTCCTC CTGCAGAGAT TCATCAAGAT CCTCGATAGT 
GTCCTGCACC ACCTGGTACC TGCCTGGGAC CACACACTGG GCACCTTCAG TGAGATTAAG 
CAAGTGAAGC AGTTCCTACT GCTGTCCCGC CAGCGGCCAG GCCTGGTGGC TCAGTGCCTG 
CGTGACTCTG AGAGCAGCAA GCCCAGCTTC ATGCCACGCC TATACATCAA CCGCCGTCTT 
GCCATGGAAC ACCGTGCCTG CCCCTCTCGA GACCCTGCCT GCAAGAATGC AGTCTTCACC 
CAGGTATATG AAGGCCTCAA GCCCTCTGAC AAATATGAAA AGCCCCTGGA CTACAGGTGG 
CCCATGCGCT ATGACCAGTG GTGGGAGTGT AAATTTATTG CAGAAGGCAT CATTGACCAA 
GGGGGTGGTT TCCGGGACAG CCTGGCAGAT ATGTCAGAAG AGCTGTGCCC TAGCTCAGCG 
GATACCCCCG TGCCCCTGCC CTTCTTTGTA CGCACAGCCA ACCAGGGCAA TGGCACTGGT 
GAGGCTCGGG ACATGTATGT ACCCAACCCC TCCTGCCGAG ACTTTGCCAA GTATGAATGG 
ATCGGACAGC TGATGGGGGC TGCCCTTCGG GGTAAGGAGT TCCTGGTCCT GGCCCTGCCT 
GGTTTTGTGT GGAAGCAGCT TTCTGGTGAG GAGGTGAGCT GGAGCAAGGA CTTCCCAGCT 
GTGGACTCTG TGCTGGTGAA GCTCCTGGAA GTGATGGAAG GAATGGACAA GGAGACGTTT 
GAGTTCAAGT TTGGGAAGGA ACTAACATTC ACCACTGTAC TGAGTGACCA ACAGGTGGTG 
GAGCTGATCC CTGGGGGTGC AGGCATCGTC GTGGGATATG GGGACCGTTC TCGTTTCATC 
CAACTGGTCC AGAAGGCACG GCTAGAGGAG AGCAAGGAGC AGGTGGCAGC TATGCAGGCA 
GGTCTGCTGA AGGTGGTACC ACAGGCTGTG CTGGACTTGC TGACCTGGCA AGAGTTGGAG 
AAGAAAGTGT GTGGGGATCC AGAGGTCACT GTGGATGCTC TGCGCAAGCT CACCCGGTTT 
GAGGACTTCG AGCCATCTGA CTCGCGGGTG CAGTATTTCT GGGAGGCACT GAACAACTTC 
ACCAACGAGG ACCGGAGCCG CTTCCTGCGC TTTGTCACGG GCCGCAGTCG CCTGCCAGCA 
CGGATCTACA TCTACCCAGA CAAGCTGGGC TACGAGACCA CAGACGCGCT GCCCGAGTCT 
TCCACTTGCT CCAGCACCCT CTTCCTGCCA CACTATGCCA GTGCCAAGGT ATGCGAGGAG 
AAGCTCCGCT ATGCGGCCTA CAACTGCGTG GCCATCGACA CTGACATGAG CCCTTGGGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD3 ( XM_024449803.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305571.1 Homo sapiens HECT domain E3 ubiquitin protein ligase 3 (HECTD3), transcript variant X1, mRNA.

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CloneID	OHu71973
Clone ID Related Accession (Same CDS sequence)	XM_024449803.1 , XM_011542140.2
Accession Version	XM_024449803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECTD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGG GCACCATTGT CAAGAAGCTG CTACTCACAG TGGATACCAC AGATGACAAC 
TTTATGCCAA AGCGGGTGGT GGTCTATGGG GGTGAAGGGG ACAACCTGAA GAAGCTGAGT 
GACGTGAGCA TTGACGAGAC CCTCATCGGG GATGTCTGTG TCCTGGAGGA CATGACCGTC 
CACCTCCCGA TCATCGAGAT CCGCATCGTG GAGTGCCGAG ATGATGGGAT TGATGTTCGT 
CTCCGAGGGG TCAAGATCAA GTCATCTAGA CAGCGGGAAC TAGGGTTGAA TGCAGACCTG 
TTCCAGCCAA CTAGTCTGGT GCGATATCCA CGCCTAGAAG GCACCGACCC TGAAGTACTG 
TACCGCAGAG CTGTCCTCCT GCAGAGATTC ATCAAGATCC TCGATAGTGT CCTGCACCAC 
CTGGTACCTG CCTGGGACCA CACACTGGGC ACCTTCAGTG AGATTAAGCA AGTGAAGCAG 
TTCCTACTGC TGTCCCGCCA GCGGCCAGGC CTGGTGGCTC AGTGCCTGCG TGACTCTGAG 
AGCAGCAAGC CCAGCTTCAT GCCACGCCTA TACATCAACC GCCGTCTTGC CATGGAACAC 
CGTGCCTGCC CCTCTCGAGA CCCTGCCTGC AAGAATGCAG TCTTCACCCA GGTATATGAA 
GGCCTCAAGC CCTCTGACAA ATATGAAAAG CCCCTGGACT ACAGGTGGCC CATGCGCTAT 
GACCAGTGGT GGGAGTGTAA ATTTATTGCA GAAGGCATCA TTGACCAAGG GGGTGGTTTC 
CGGGACAGCC TGGCAGATAT GTCAGAAGAG CTGTGCCCTA GCTCAGCGGA TACCCCCGTG 
CCCCTGCCCT TCTTTGTACG CACAGCCAAC CAGGGCAATG GCACTGGTGA GGCTCGGGAC 
ATGTATGTAC CCAACCCCTC CTGCCGAGAC TTTGCCAAGT ATGAATGGAT CGGACAGCTG 
ATGGGGGCTG CCCTTCGGGG TAAGGAGTTC CTGGTCCTGG CCCTGCCTGG TTTTGTGTGG 
AAGCAGCTTT CTGGTGAGGA GGTGAGCTGG AGCAAGGACT TCCCAGCTGT GGACTCTGTG 
CTGGTGAAGC TCCTGGAAGT GATGGAAGGA ATGGACAAGG AGACGTTTGA GTTCAAGTTT 
GGGAAGGAAC TAACATTCAC CACTGTACTG AGTGACCAAC AGGTGGTGGA GCTGATCCCT 
GGGGGTGCAG GCATCGTCGT GGGATATGGG GACCGTTCTC GTTTCATCCA ACTGGTCCAG 
AAGGCACGGC TAGAGGAGAG CAAGGAGCAG GTGGCAGCTA TGCAGGCAGG TCTGCTGAAG 
GTGGTACCAC AGGCTGTGCT GGACTTGCTG ACCTGGCAAG AGTTGGAGAA GAAAGTGTGT 
GGGGATCCAG AGGTCACTGT GGATGCTCTG CGCAAGCTCA CCCGGTTTGA GGACTTCGAG 
CCATCTGACT CGCGGGTGCA GTATTTCTGG GAGGCACTGA ACAACTTCAC CAACGAGGAC 
CGGAGCCGCT TCCTGCGCTT TGTCACGGGC CGCAGTCGCC TGCCAGCACG GATCTACATC 
TACCCAGACA AGCTGGGCTA CGAGACCACA GACGCGCTGC CCGAGTCTTC CACTTGCTCC 
AGCACCCTCT TCCTGCCACA CTATGCCAGT GCCAAGGTAT GCGAGGAGAA GCTCCGCTAT 
GCGGCCTACA ACTGCGTGGC CATCGACACT GACATGAGCC CTTGGGAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305571.1
CDS	754..2487
Translation	MKKGTIVKKLLLTVDTTDDNFMPKRVVVYGGEGDNLKKLSDVSIDETLIGDVCVLEDMTVHLPIIEIRIVECRDDGIDVRLRGVKIKSSRQRELGLNADLFQPTSLVRYPRLEGTDPEVLYRRAVLLQRFIKILDSVLHHLVPAWDHTLGTFSEIKQVKQFLLLSRQRPGLVAQCLRDSESSKPSFMPRLYINRRLAMEHRACPSRDPACKNAVFTQVYEGLKPSDKYEKPLDYRWPMRYDQWWECKFIAEGIIDQGGGFRDSLADMSEELCPSSADTPVPLPFFVRTANQGNGTGEARDMYVPNPSCRDFAKYEWIGQLMGAALRGKEFLVLALPGFVWKQLSGEEVSWSKDFPAVDSVLVKLLEVMEGMDKETFEFKFGKELTFTTVLSDQQVVELIPGGAGIVVGYGDRSRFIQLVQKARLEESKEQVAAMQAGLLKVVPQAVLDLLTWQELEKKVCGDPEVTVDALRKLTRFEDFEPSDSRVQYFWEALNNFTNEDRSRFLRFVTGRSRLPARIYIYPDKLGYETTDALPESSTCSSTLFLPHYASAKVCEEKLRYAAYNCVAIDTDMSPWEE

Target ORF information:

RefSeq Version	XM_024449803.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain E3 ubiquitin protein ligase 3 (HECTD3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449803.1

ORF Insert Sequence:

ATGAAGAAGG GCACCATTGT CAAGAAGCTG CTACTCACAG TGGATACCAC AGATGACAAC 
TTTATGCCAA AGCGGGTGGT GGTCTATGGG GGTGAAGGGG ACAACCTGAA GAAGCTGAGT 
GACGTGAGCA TTGACGAGAC CCTCATCGGG GATGTCTGTG TCCTGGAGGA CATGACCGTC 
CACCTCCCGA TCATCGAGAT CCGCATCGTG GAGTGCCGAG ATGATGGGAT TGATGTTCGT 
CTCCGAGGGG TCAAGATCAA GTCATCTAGA CAGCGGGAAC TAGGGTTGAA TGCAGACCTG 
TTCCAGCCAA CTAGTCTGGT GCGATATCCA CGCCTAGAAG GCACCGACCC TGAAGTACTG 
TACCGCAGAG CTGTCCTCCT GCAGAGATTC ATCAAGATCC TCGATAGTGT CCTGCACCAC 
CTGGTACCTG CCTGGGACCA CACACTGGGC ACCTTCAGTG AGATTAAGCA AGTGAAGCAG 
TTCCTACTGC TGTCCCGCCA GCGGCCAGGC CTGGTGGCTC AGTGCCTGCG TGACTCTGAG 
AGCAGCAAGC CCAGCTTCAT GCCACGCCTA TACATCAACC GCCGTCTTGC CATGGAACAC 
CGTGCCTGCC CCTCTCGAGA CCCTGCCTGC AAGAATGCAG TCTTCACCCA GGTATATGAA 
GGCCTCAAGC CCTCTGACAA ATATGAAAAG CCCCTGGACT ACAGGTGGCC CATGCGCTAT 
GACCAGTGGT GGGAGTGTAA ATTTATTGCA GAAGGCATCA TTGACCAAGG GGGTGGTTTC 
CGGGACAGCC TGGCAGATAT GTCAGAAGAG CTGTGCCCTA GCTCAGCGGA TACCCCCGTG 
CCCCTGCCCT TCTTTGTACG CACAGCCAAC CAGGGCAATG GCACTGGTGA GGCTCGGGAC 
ATGTATGTAC CCAACCCCTC CTGCCGAGAC TTTGCCAAGT ATGAATGGAT CGGACAGCTG 
ATGGGGGCTG CCCTTCGGGG TAAGGAGTTC CTGGTCCTGG CCCTGCCTGG TTTTGTGTGG 
AAGCAGCTTT CTGGTGAGGA GGTGAGCTGG AGCAAGGACT TCCCAGCTGT GGACTCTGTG 
CTGGTGAAGC TCCTGGAAGT GATGGAAGGA ATGGACAAGG AGACGTTTGA GTTCAAGTTT 
GGGAAGGAAC TAACATTCAC CACTGTACTG AGTGACCAAC AGGTGGTGGA GCTGATCCCT 
GGGGGTGCAG GCATCGTCGT GGGATATGGG GACCGTTCTC GTTTCATCCA ACTGGTCCAG 
AAGGCACGGC TAGAGGAGAG CAAGGAGCAG GTGGCAGCTA TGCAGGCAGG TCTGCTGAAG 
GTGGTACCAC AGGCTGTGCT GGACTTGCTG ACCTGGCAAG AGTTGGAGAA GAAAGTGTGT 
GGGGATCCAG AGGTCACTGT GGATGCTCTG CGCAAGCTCA CCCGGTTTGA GGACTTCGAG 
CCATCTGACT CGCGGGTGCA GTATTTCTGG GAGGCACTGA ACAACTTCAC CAACGAGGAC 
CGGAGCCGCT TCCTGCGCTT TGTCACGGGC CGCAGTCGCC TGCCAGCACG GATCTACATC 
TACCCAGACA AGCTGGGCTA CGAGACCACA GACGCGCTGC CCGAGTCTTC CACTTGCTCC 
AGCACCCTCT TCCTGCCACA CTATGCCAGT GCCAAGGTAT GCGAGGAGAA GCTCCGCTAT 
GCGGCCTACA ACTGCGTGGC CATCGACACT GACATGAGCC CTTGGGAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD3 ( XM_011542140.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540442.1 Homo sapiens HECT domain E3 ubiquitin protein ligase 3 (HECTD3), transcript variant X1, mRNA.

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CloneID	OHu71973
Clone ID Related Accession (Same CDS sequence)	XM_024449803.1 , XM_011542140.2
Accession Version	XM_011542140.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECTD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542140.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGG GCACCATTGT CAAGAAGCTG CTACTCACAG TGGATACCAC AGATGACAAC 
TTTATGCCAA AGCGGGTGGT GGTCTATGGG GGTGAAGGGG ACAACCTGAA GAAGCTGAGT 
GACGTGAGCA TTGACGAGAC CCTCATCGGG GATGTCTGTG TCCTGGAGGA CATGACCGTC 
CACCTCCCGA TCATCGAGAT CCGCATCGTG GAGTGCCGAG ATGATGGGAT TGATGTTCGT 
CTCCGAGGGG TCAAGATCAA GTCATCTAGA CAGCGGGAAC TAGGGTTGAA TGCAGACCTG 
TTCCAGCCAA CTAGTCTGGT GCGATATCCA CGCCTAGAAG GCACCGACCC TGAAGTACTG 
TACCGCAGAG CTGTCCTCCT GCAGAGATTC ATCAAGATCC TCGATAGTGT CCTGCACCAC 
CTGGTACCTG CCTGGGACCA CACACTGGGC ACCTTCAGTG AGATTAAGCA AGTGAAGCAG 
TTCCTACTGC TGTCCCGCCA GCGGCCAGGC CTGGTGGCTC AGTGCCTGCG TGACTCTGAG 
AGCAGCAAGC CCAGCTTCAT GCCACGCCTA TACATCAACC GCCGTCTTGC CATGGAACAC 
CGTGCCTGCC CCTCTCGAGA CCCTGCCTGC AAGAATGCAG TCTTCACCCA GGTATATGAA 
GGCCTCAAGC CCTCTGACAA ATATGAAAAG CCCCTGGACT ACAGGTGGCC CATGCGCTAT 
GACCAGTGGT GGGAGTGTAA ATTTATTGCA GAAGGCATCA TTGACCAAGG GGGTGGTTTC 
CGGGACAGCC TGGCAGATAT GTCAGAAGAG CTGTGCCCTA GCTCAGCGGA TACCCCCGTG 
CCCCTGCCCT TCTTTGTACG CACAGCCAAC CAGGGCAATG GCACTGGTGA GGCTCGGGAC 
ATGTATGTAC CCAACCCCTC CTGCCGAGAC TTTGCCAAGT ATGAATGGAT CGGACAGCTG 
ATGGGGGCTG CCCTTCGGGG TAAGGAGTTC CTGGTCCTGG CCCTGCCTGG TTTTGTGTGG 
AAGCAGCTTT CTGGTGAGGA GGTGAGCTGG AGCAAGGACT TCCCAGCTGT GGACTCTGTG 
CTGGTGAAGC TCCTGGAAGT GATGGAAGGA ATGGACAAGG AGACGTTTGA GTTCAAGTTT 
GGGAAGGAAC TAACATTCAC CACTGTACTG AGTGACCAAC AGGTGGTGGA GCTGATCCCT 
GGGGGTGCAG GCATCGTCGT GGGATATGGG GACCGTTCTC GTTTCATCCA ACTGGTCCAG 
AAGGCACGGC TAGAGGAGAG CAAGGAGCAG GTGGCAGCTA TGCAGGCAGG TCTGCTGAAG 
GTGGTACCAC AGGCTGTGCT GGACTTGCTG ACCTGGCAAG AGTTGGAGAA GAAAGTGTGT 
GGGGATCCAG AGGTCACTGT GGATGCTCTG CGCAAGCTCA CCCGGTTTGA GGACTTCGAG 
CCATCTGACT CGCGGGTGCA GTATTTCTGG GAGGCACTGA ACAACTTCAC CAACGAGGAC 
CGGAGCCGCT TCCTGCGCTT TGTCACGGGC CGCAGTCGCC TGCCAGCACG GATCTACATC 
TACCCAGACA AGCTGGGCTA CGAGACCACA GACGCGCTGC CCGAGTCTTC CACTTGCTCC 
AGCACCCTCT TCCTGCCACA CTATGCCAGT GCCAAGGTAT GCGAGGAGAA GCTCCGCTAT 
GCGGCCTACA ACTGCGTGGC CATCGACACT GACATGAGCC CTTGGGAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540442.1
CDS	348..2081
Translation	MKKGTIVKKLLLTVDTTDDNFMPKRVVVYGGEGDNLKKLSDVSIDETLIGDVCVLEDMTVHLPIIEIRIVECRDDGIDVRLRGVKIKSSRQRELGLNADLFQPTSLVRYPRLEGTDPEVLYRRAVLLQRFIKILDSVLHHLVPAWDHTLGTFSEIKQVKQFLLLSRQRPGLVAQCLRDSESSKPSFMPRLYINRRLAMEHRACPSRDPACKNAVFTQVYEGLKPSDKYEKPLDYRWPMRYDQWWECKFIAEGIIDQGGGFRDSLADMSEELCPSSADTPVPLPFFVRTANQGNGTGEARDMYVPNPSCRDFAKYEWIGQLMGAALRGKEFLVLALPGFVWKQLSGEEVSWSKDFPAVDSVLVKLLEVMEGMDKETFEFKFGKELTFTTVLSDQQVVELIPGGAGIVVGYGDRSRFIQLVQKARLEESKEQVAAMQAGLLKVVPQAVLDLLTWQELEKKVCGDPEVTVDALRKLTRFEDFEPSDSRVQYFWEALNNFTNEDRSRFLRFVTGRSRLPARIYIYPDKLGYETTDALPESSTCSSTLFLPHYASAKVCEEKLRYAAYNCVAIDTDMSPWEE

Target ORF information:

RefSeq Version	XM_011542140.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain E3 ubiquitin protein ligase 3 (HECTD3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542140.2

ORF Insert Sequence:

ATGAAGAAGG GCACCATTGT CAAGAAGCTG CTACTCACAG TGGATACCAC AGATGACAAC 
TTTATGCCAA AGCGGGTGGT GGTCTATGGG GGTGAAGGGG ACAACCTGAA GAAGCTGAGT 
GACGTGAGCA TTGACGAGAC CCTCATCGGG GATGTCTGTG TCCTGGAGGA CATGACCGTC 
CACCTCCCGA TCATCGAGAT CCGCATCGTG GAGTGCCGAG ATGATGGGAT TGATGTTCGT 
CTCCGAGGGG TCAAGATCAA GTCATCTAGA CAGCGGGAAC TAGGGTTGAA TGCAGACCTG 
TTCCAGCCAA CTAGTCTGGT GCGATATCCA CGCCTAGAAG GCACCGACCC TGAAGTACTG 
TACCGCAGAG CTGTCCTCCT GCAGAGATTC ATCAAGATCC TCGATAGTGT CCTGCACCAC 
CTGGTACCTG CCTGGGACCA CACACTGGGC ACCTTCAGTG AGATTAAGCA AGTGAAGCAG 
TTCCTACTGC TGTCCCGCCA GCGGCCAGGC CTGGTGGCTC AGTGCCTGCG TGACTCTGAG 
AGCAGCAAGC CCAGCTTCAT GCCACGCCTA TACATCAACC GCCGTCTTGC CATGGAACAC 
CGTGCCTGCC CCTCTCGAGA CCCTGCCTGC AAGAATGCAG TCTTCACCCA GGTATATGAA 
GGCCTCAAGC CCTCTGACAA ATATGAAAAG CCCCTGGACT ACAGGTGGCC CATGCGCTAT 
GACCAGTGGT GGGAGTGTAA ATTTATTGCA GAAGGCATCA TTGACCAAGG GGGTGGTTTC 
CGGGACAGCC TGGCAGATAT GTCAGAAGAG CTGTGCCCTA GCTCAGCGGA TACCCCCGTG 
CCCCTGCCCT TCTTTGTACG CACAGCCAAC CAGGGCAATG GCACTGGTGA GGCTCGGGAC 
ATGTATGTAC CCAACCCCTC CTGCCGAGAC TTTGCCAAGT ATGAATGGAT CGGACAGCTG 
ATGGGGGCTG CCCTTCGGGG TAAGGAGTTC CTGGTCCTGG CCCTGCCTGG TTTTGTGTGG 
AAGCAGCTTT CTGGTGAGGA GGTGAGCTGG AGCAAGGACT TCCCAGCTGT GGACTCTGTG 
CTGGTGAAGC TCCTGGAAGT GATGGAAGGA ATGGACAAGG AGACGTTTGA GTTCAAGTTT 
GGGAAGGAAC TAACATTCAC CACTGTACTG AGTGACCAAC AGGTGGTGGA GCTGATCCCT 
GGGGGTGCAG GCATCGTCGT GGGATATGGG GACCGTTCTC GTTTCATCCA ACTGGTCCAG 
AAGGCACGGC TAGAGGAGAG CAAGGAGCAG GTGGCAGCTA TGCAGGCAGG TCTGCTGAAG 
GTGGTACCAC AGGCTGTGCT GGACTTGCTG ACCTGGCAAG AGTTGGAGAA GAAAGTGTGT 
GGGGATCCAG AGGTCACTGT GGATGCTCTG CGCAAGCTCA CCCGGTTTGA GGACTTCGAG 
CCATCTGACT CGCGGGTGCA GTATTTCTGG GAGGCACTGA ACAACTTCAC CAACGAGGAC 
CGGAGCCGCT TCCTGCGCTT TGTCACGGGC CGCAGTCGCC TGCCAGCACG GATCTACATC 
TACCCAGACA AGCTGGGCTA CGAGACCACA GACGCGCTGC CCGAGTCTTC CACTTGCTCC 
AGCACCCTCT TCCTGCCACA CTATGCCAGT GCCAAGGTAT GCGAGGAGAA GCTCCGCTAT 
GCGGCCTACA ACTGCGTGGC CATCGACACT GACATGAGCC CTTGGGAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HECTD3 Mediates an HSP90-Dependent Degradation Pathway for Protein Kinase Clients.
Cell reports19(12)2515-2528(2017 Jun)
Li Z,Zhou L,Prodromou C,Savic V,Pearl LH

The HECTD3 E3 ubiquitin ligase suppresses cisplatin-induced apoptosis via stabilizing MALT1.
Neoplasia (New York, N.Y.)15(1)39-48(2013 Jan)
Li Y,Chen X,Wang Z,Zhao D,Chen H,Chen W,Zhou Z,Zhang J,Zhang J,Li H,Chen C

The E3 ubiquitin ligase HECTD3 regulates ubiquitination and degradation of Tara.
Biochemical and biophysical research communications367(4)805-12(2008 Mar)
Yu J,Lan J,Zhu Y,Li X,Lai X,Xue Y,Jin C,Huang H

Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.
Nature biotechnology21(5)566-9(2003 May)
Gevaert K,Goethals M,Martens L,Van Damme J,Staes A,Thomas GR,Vandekerckhove J

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HECTD3 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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