hectd3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	hectd3
Entrez Gene ID	779952
Full Name	HECT domain containing E3 ubiquitin protein ligase 3
General protein information	Preferred Names HECT domain containing E3 ubiquitin protein ligase 3 Names E3 ubiquitin-protein ligase HECTD3 HECT domain containing 3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012961325.2	XP_012816779.1	E3 ubiquitin-protein ligase HECTD3 isoform X1
NM_001079029.1	NP_001072497.1	E3 ubiquitin-protein ligase HECTD3
XM_012961326.3	XP_012816780.2	E3 ubiquitin-protein ligase HECTD3 isoform X1
XM_012961326.2	XP_012816780.1	E3 ubiquitin-protein ligase HECTD3 isoform X1
XM_012961325.3	XP_012816779.2	E3 ubiquitin-protein ligase HECTD3 isoform X1

Pathways from BioSystems

Homologies

	HECTD3	XP_001100268.1
Canis lupus familiaris (dog)	HECTD3	XP_539635.2
Bos taurus (cattle)	HECTD3	NP_001092431.1
Mus musculus (house mouse)	Hectd3	NP_780453.1
Gallus gallus (chicken)	HECTD3	XP_422429.2
Danio rerio (zebrafish)	hectd3	NP_001070627.2
Xenopus tropicalis (tropical clawed frog)	hectd3	NP_001072497.1
Homo sapiens (human)	HECTD3	NP_078878.3
Pan troglodytes (chimpanzee)	HECTD3	XP_513128.4
Rattus norvegicus (Norway rat)	Hectd3	XP_003750059.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hectd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hectd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa22779	NM_001079029.1 Latest version!	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa29310	XM_012961325.2 Latest version!	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa29310	XM_012961326.2 Latest version!	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa56159	XM_012961325.3 Latest version!	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa56159	XM_012961326.3 Latest version!	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hectd3 ( NM_001079029.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072497.1 Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), mRNA.

Price & Availability↑

CloneID	OXa22779
Clone ID Related Accession (Same CDS sequence)	NM_001079029.1
Accession Version	NM_001079029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECTD3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121928.1. ##Evidence-Data-START## Transcript exon combination :: BC121928.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGCAGGCTC CAGATAATCC ACATCGACTA CTGGGCCAAA TCCGCTGTGT TTTGGAGTGT 
ATCGACTGCT TCCGACATGT GCAGCCCCTC CCCGAGGCCT TATGTTATGT TCCTGGGGAA 
GTGCAGTACA AAATCTGCAA GGACACCAGC TCATCATCAT CATCTCGTTC CCTGTTGACT 
GTTTGGGAGG CACCTGGATA TGCCAAGCAA AACTTGAAGA AATTGTCTCG GGCAACAGTT 
GAAGTGCGCA AAGGCAGCTG TGTCACAGCC ACAGGGGAGG AATACTGCAA TGCTAATGGA 
CTGTGGGTAA AGCTGAGCGA GGCACAAATG GAAGAATTTA AAGGTAGCTG TGACATGGCA 
GAGGGCTGGG TCTTGCTATG TCGGCAAGAT GAAGGAGGAG ACAGGCTTAT TCCTGTGGAG 
TCTACAGATA AAACCACGAG GCAGCAACAG GTGTTTGGGA TAGACTACAA ATCAGTCCAC 
ATGTGGGAGA AGGTTGTTGA TGCAACTTAT TCTATGCACT TAGGGGCCAA ACCTAAAATC 
ATTAAGCCAG ATGAAGAGGC AGTTCAAAAA CTGCGCTTCC TTCCTCCATC TTGGACCTAT 
GAATGTGATG AAGATCTGGT GCACTTTCTT TTTGAGCACG TTGTAAAGCA AGATGAGAGC 
TTAGGAAGTG TGAAGCAGTA TGTAGAAAGC ATCAGTGTGT CCTCTGCTAC GGTGGAAAAC 
AACTTGTCGT GTTTGACAGA TGGTTACCAT GACACATATT GGGAGAGCGA TGGCTCCCAC 
GGTGAGCACT GGATCCAACT ACACATGAAA GAAGGCACCA TTGTCAAGAG ACTCCTGATA 
ACTGTGGACT CTACAGATGA ATCGTACATG CCCAAAGGGA TTGTGGTATA TGGAGGTGAG 
AGAGATGCCC TAAAAAAAAT CAACGAAGTC TCCTTAGACG AAAACCTGAT TGGAGATGTT 
TGTATCCTGG AAGATATGAC CACTCACCTA CCAATCATTG AGATCAAAAT CACACAGTGT 
CTGGATGAAG GAATTGATGT CCGGATCAGA GGGCTCAAGA TTAAATCTTC AAAAAGGCGT 
GATTTAGGCC TCACTGCTGA TCTTTTCCAG CCAAACAACT TAGTTCGGTA TCCACGCTTG 
GAGGCCATTG AGCCAGATGT GCTTTATCAT AGAGCTGTGG TAGTCCAGAG GTTTGTTAAG 
TTGCTGGATA ATGTGCTAAA TCACATGGTA CCGGCCTGGG ATCACAGCAT TGGCACATTC 
AATCAGCTAA AGCACATCAA ACAGTTCCTG CTGCTATCCA AATGCAGATC TTCACTTATT 
ACTCAGTGTC TAAAGGATTC AGAAACCATG AAACCCGACT TCATGCCCTG TTTATATATT 
AACCGTAGGA TTGCAGTGGA GCATCGGGAC AATCCTTCAT TGGACCCTTC ATACAAGAAG 
ACTGTTTTCA GTCAGGTATA TGAAGGCCTA AAGCTTACAG ATAAATCAGA GAAACCACTG 
GATTACAGGT GGCCTGTAAG GTATGAGCAA TGGTGGGAGT GTAAATTTAT TGCAGAAGGA 
ATTATTGATC AAGGGGGAGG TTTTAGGGAC AGTCTGGCTG ATATATCTGA GGAGCTTTGC 
CCAAGTTCTG CTGATATACC TGTTCCACTA CCATTTTTTG TACGCACTTC AAACCAGGGT 
AACGGAACTG GGGAAGCACA AGACATGTAT GTGCCAAATC CATCATGTAA GGACCTGGCA 
AAATATGAGT GGATTGGACA ACTTATGGGA GCAGCACTGA GAGGAAAGGA ATTCTTGGTT 
CTAGCTCTGC CAGGCCTGGT GTGGAAACAG TTGACTGGAG AAGAAGTAAG CTGGAGTAAG 
GATTTTCCTG CAGTGGATTC TTTACTTGTG AAACTTCTGG AGATGATGGA ACTTATGGAC 
GAAGAAACAT TTGAGTTTAA GTTTAGTGGT GAGCTGACAT ACACCACTGT CCTGAGTGAT 
CAAAAGATGG TGGAGCTGGT TCCCGGAGGC AGCAACATAT CCGTGCTCTA TAAGGACCGT 
GTGGAATTTA TTAGAATGGT GCAGAAAGCA CGACTGGAGG AGAGTAAAGA ACAGATAGGA 
GCTCTCCAGG CTGGCCTTTT GAAGGTTGTC CCACAAGCAG TGCTAGATCT TCTTACATGG 
CAAGAACTTG AGAAAAGGGT TTGTGGGGAT CCAGAAATCA CTGTTGAAGC CCTAAAAAAA 
CTAACCAGGT TTGGGGACAT AGAACAAACA GACACTCGGG TCCAGTATTT CTGGGAGGCA 
CTAAACAATT TCACTAATGA GGACCGCAGC CGTTTCCTAA GGTTTGTGAC TGGCCGGAGT 
CGACTTCCAG CACCAATTTA TATCTACCCA GATAGATCAT GGCTGGGTAC AGACGACTCC 
CTACCTGAAT CATCCACCTG TTCTAGTTCC TTGTATCTGC CAAATTATGC CAGTGCAAAA 
ATTTGTGAAG AGAAACTGCG TTATGCTGCA TATAACTGTG TAGCCATCGA TACAGATATG 
AATCCATGGG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072497.1
CDS	192..2729
Translation	MQAPDNPHRLLGQIRCVLECIDCFRHVQPLPEALCYVPGEVQYKICKDTSSSSSSRSLLTVWEAPGYAKQNLKKLSRATVEVRKGSCVTATGEEYCNANGLWVKLSEAQMEEFKGSCDMAEGWVLLCRQDEGGDRLIPVESTDKTTRQQQVFGIDYKSVHMWEKVVDATYSMHLGAKPKIIKPDEEAVQKLRFLPPSWTYECDEDLVHFLFEHVVKQDESLGSVKQYVESISVSSATVENNLSCLTDGYHDTYWESDGSHGEHWIQLHMKEGTIVKRLLITVDSTDESYMPKGIVVYGGERDALKKINEVSLDENLIGDVCILEDMTTHLPIIEIKITQCLDEGIDVRIRGLKIKSSKRRDLGLTADLFQPNNLVRYPRLEAIEPDVLYHRAVVVQRFVKLLDNVLNHMVPAWDHSIGTFNQLKHIKQFLLLSKCRSSLITQCLKDSETMKPDFMPCLYINRRIAVEHRDNPSLDPSYKKTVFSQVYEGLKLTDKSEKPLDYRWPVRYEQWWECKFIAEGIIDQGGGFRDSLADISEELCPSSADIPVPLPFFVRTSNQGNGTGEAQDMYVPNPSCKDLAKYEWIGQLMGAALRGKEFLVLALPGLVWKQLTGEEVSWSKDFPAVDSLLVKLLEMMELMDEETFEFKFSGELTYTTVLSDQKMVELVPGGSNISVLYKDRVEFIRMVQKARLEESKEQIGALQAGLLKVVPQAVLDLLTWQELEKRVCGDPEITVEALKKLTRFGDIEQTDTRVQYFWEALNNFTNEDRSRFLRFVTGRSRLPAPIYIYPDRSWLGTDDSLPESSTCSSSLYLPNYASAKICEEKLRYAAYNCVAIDTDMNPWEE

Target ORF information:

RefSeq Version	NM_001079029.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079029.1

ORF Insert Sequence:

ATGCAGGCTC CAGATAATCC ACATCGACTA CTGGGCCAAA TCCGCTGTGT TTTGGAGTGT 
ATCGACTGCT TCCGACATGT GCAGCCCCTC CCCGAGGCCT TATGTTATGT TCCTGGGGAA 
GTGCAGTACA AAATCTGCAA GGACACCAGC TCATCATCAT CATCTCGTTC CCTGTTGACT 
GTTTGGGAGG CACCTGGATA TGCCAAGCAA AACTTGAAGA AATTGTCTCG GGCAACAGTT 
GAAGTGCGCA AAGGCAGCTG TGTCACAGCC ACAGGGGAGG AATACTGCAA TGCTAATGGA 
CTGTGGGTAA AGCTGAGCGA GGCACAAATG GAAGAATTTA AAGGTAGCTG TGACATGGCA 
GAGGGCTGGG TCTTGCTATG TCGGCAAGAT GAAGGAGGAG ACAGGCTTAT TCCTGTGGAG 
TCTACAGATA AAACCACGAG GCAGCAACAG GTGTTTGGGA TAGACTACAA ATCAGTCCAC 
ATGTGGGAGA AGGTTGTTGA TGCAACTTAT TCTATGCACT TAGGGGCCAA ACCTAAAATC 
ATTAAGCCAG ATGAAGAGGC AGTTCAAAAA CTGCGCTTCC TTCCTCCATC TTGGACCTAT 
GAATGTGATG AAGATCTGGT GCACTTTCTT TTTGAGCACG TTGTAAAGCA AGATGAGAGC 
TTAGGAAGTG TGAAGCAGTA TGTAGAAAGC ATCAGTGTGT CCTCTGCTAC GGTGGAAAAC 
AACTTGTCGT GTTTGACAGA TGGTTACCAT GACACATATT GGGAGAGCGA TGGCTCCCAC 
GGTGAGCACT GGATCCAACT ACACATGAAA GAAGGCACCA TTGTCAAGAG ACTCCTGATA 
ACTGTGGACT CTACAGATGA ATCGTACATG CCCAAAGGGA TTGTGGTATA TGGAGGTGAG 
AGAGATGCCC TAAAAAAAAT CAACGAAGTC TCCTTAGACG AAAACCTGAT TGGAGATGTT 
TGTATCCTGG AAGATATGAC CACTCACCTA CCAATCATTG AGATCAAAAT CACACAGTGT 
CTGGATGAAG GAATTGATGT CCGGATCAGA GGGCTCAAGA TTAAATCTTC AAAAAGGCGT 
GATTTAGGCC TCACTGCTGA TCTTTTCCAG CCAAACAACT TAGTTCGGTA TCCACGCTTG 
GAGGCCATTG AGCCAGATGT GCTTTATCAT AGAGCTGTGG TAGTCCAGAG GTTTGTTAAG 
TTGCTGGATA ATGTGCTAAA TCACATGGTA CCGGCCTGGG ATCACAGCAT TGGCACATTC 
AATCAGCTAA AGCACATCAA ACAGTTCCTG CTGCTATCCA AATGCAGATC TTCACTTATT 
ACTCAGTGTC TAAAGGATTC AGAAACCATG AAACCCGACT TCATGCCCTG TTTATATATT 
AACCGTAGGA TTGCAGTGGA GCATCGGGAC AATCCTTCAT TGGACCCTTC ATACAAGAAG 
ACTGTTTTCA GTCAGGTATA TGAAGGCCTA AAGCTTACAG ATAAATCAGA GAAACCACTG 
GATTACAGGT GGCCTGTAAG GTATGAGCAA TGGTGGGAGT GTAAATTTAT TGCAGAAGGA 
ATTATTGATC AAGGGGGAGG TTTTAGGGAC AGTCTGGCTG ATATATCTGA GGAGCTTTGC 
CCAAGTTCTG CTGATATACC TGTTCCACTA CCATTTTTTG TACGCACTTC AAACCAGGGT 
AACGGAACTG GGGAAGCACA AGACATGTAT GTGCCAAATC CATCATGTAA GGACCTGGCA 
AAATATGAGT GGATTGGACA ACTTATGGGA GCAGCACTGA GAGGAAAGGA ATTCTTGGTT 
CTAGCTCTGC CAGGCCTGGT GTGGAAACAG TTGACTGGAG AAGAAGTAAG CTGGAGTAAG 
GATTTTCCTG CAGTGGATTC TTTACTTGTG AAACTTCTGG AGATGATGGA ACTTATGGAC 
GAAGAAACAT TTGAGTTTAA GTTTAGTGGT GAGCTGACAT ACACCACTGT CCTGAGTGAT 
CAAAAGATGG TGGAGCTGGT TCCCGGAGGC AGCAACATAT CCGTGCTCTA TAAGGACCGT 
GTGGAATTTA TTAGAATGGT GCAGAAAGCA CGACTGGAGG AGAGTAAAGA ACAGATAGGA 
GCTCTCCAGG CTGGCCTTTT GAAGGTTGTC CCACAAGCAG TGCTAGATCT TCTTACATGG 
CAAGAACTTG AGAAAAGGGT TTGTGGGGAT CCAGAAATCA CTGTTGAAGC CCTAAAAAAA 
CTAACCAGGT TTGGGGACAT AGAACAAACA GACACTCGGG TCCAGTATTT CTGGGAGGCA 
CTAAACAATT TCACTAATGA GGACCGCAGC CGTTTCCTAA GGTTTGTGAC TGGCCGGAGT 
CGACTTCCAG CACCAATTTA TATCTACCCA GATAGATCAT GGCTGGGTAC AGACGACTCC 
CTACCTGAAT CATCCACCTG TTCTAGTTCC TTGTATCTGC CAAATTATGC CAGTGCAAAA 
ATTTGTGAAG AGAAACTGCG TTATGCTGCA TATAACTGTG TAGCCATCGA TACAGATATG 
AATCCATGGG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd3 ( XM_012961325.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816779.1 Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa29310
Clone ID Related Accession (Same CDS sequence)	XM_012961325.2 , XM_012961326.2
Accession Version	XM_012961325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECTD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCTC CAGATAATCC ACATCGACTA CTGGGCCAAA TCCGCTGTGT TTTGGAGTGT 
ATCGACTGCT TCCGACATGT GCAGCCCCTC CCCGAGGCCT TATGTTATGT TCCTGGGGAA 
GTGCAGTACA AAATCTGCAA GGACACCAGC TCATCATCAT CATCTCGTTC CCTGTTGACT 
GTTTGGGAGG CACCTGGATA TGCCAAGCAA AACTTGAAGA AATTGTCTCG GGCAACAGTT 
GAAGTGCGCA AAGGCAGCTG TGTCACAGCC ACAGGGGAGG AATACTGCAA TGCTAATGGA 
CTGTGGGTAA AGCTGAGCGA GGCACAAATG GAAGAATTTA AAGGTAGCTG TGACATGGCA 
GAGGGCTGGG TCTTGCTATG TCGGCAAGAT GAAGGAGGAG ACAGGCTTAT TCCTGTGGAG 
TCTAGAGATA AAACCACGAG GCAGCAACAG GTGTTTGGGA TAGACTACAA ATCAGTCCAC 
ATGTGGGAGA AGGTTGTTGA TGCAACTTAT TCTATGCACT TAGGGGCCAA ACCTAAAATC 
ATTAAGCCAG ATGAAGAGGC AGTTCAAAAA CTGCGCTTCC TTCCTCCATC TTGGACCTAT 
GAATGTGATG AAGATCTGGT GCACTTTCTT TTTGAGCACG TTGTAAAGCA AGATGAGAGC 
TTAGGAAGTG TGAAGCAGTA TGTAGAAAGC ATCAGTGTGT CCTCTGCTAC GGTGGAAAAC 
AACTTGTCGT GTTTGACAGA TGGTTACCAT GACACATATT GGGAGAGCGA TGGCTCCCAC 
GGTGAGCACT GGATCCAACT ACACATGAAA GAAGGCACCA TTGTCAAGAG ACTCCTGATA 
ACTGTGGACT CTACAGATGA ATCGTACATG CCCAAAGGGA TTGTGGTATA TGGAGGTGAG 
AGAGATGCCC TGAAAAAAAT CAACGAAGTC TCCTTAGACG AAAACCTGAT TGGAGATGTT 
TGTATCCTGG AAGATATGAC CACTCACCTA CCAATCATTG AGATCAAAAT CACACAGTGT 
CTGGATGAAG GAATTGATGT CCGGATCAGA GGGCTCAAGA TTAAATCTTC AAAAAGGCGT 
GATTTAGGCC TCACTGCTGA TCTTTTCCAG CCAAACAACT TAGTTCGGTA TCCACGCTTG 
GAGGCCATTG AGCCAGATGT GCTTTATCAT AGAGCTGTGG TAGTCCAGAG GTTTGTTAAG 
TTGCTGGATA ATGTGCTAAA TCACATGGTA CCGGCCTGGG ATCACAGCAT TGGCACATTC 
AATCAGCTAA AGCACATCAA ACAGTTCCTG CTGCTATCCA AATGCAGATC TTCACTTATT 
ACTCAGTGTC TAAAGGATTC AGAAACCATG AAACCCGACT TCATGCCCTG TTTATATATT 
AACCGTAGGA TTGCAGTGGA GCATCGGGAC AATCCTTCAT TGGACCCTTC ATACAAGAAG 
ACTGTTTTCA GTCAGGTATA TGAAGGCCTA AAGCTTACAG ATAAATCAGA GAAACCACTG 
GATTACAGGT GGCCTGTAAG GTATGAGCAA TGGTGGGAGT GTAAATTTAT TGCAGAAGGA 
ATTATTGATC AAGGGGGAGG TTTTAGGGAC AGTCTGGCTG ATATATCTGA GGAGCTTTGC 
CCAAGTTCTG CTGATATACC TGTTCCACTA CCATTTTTTG TACGCACTTC AAACCAGGGT 
AACGGAACTG GGGAAGCACA AGACATGTAT GTGCCAAATC CATCATGTAA GGACCTGGCA 
AAATATGAGT GGATTGGACA ACTTATGGGA GCAGCACTGA GAGGAAAGGA ATTCTTGGTT 
CTAGCTCTGC CAGGCCTGGT GTGGAAACAG TTGACTGGAG AAGAAGTAAG CTGGAGTAAG 
GATTTTCCTG CAGTGGATTC TTTACTTGTG AAACTTCTGG AGATGATGGA ACTTATGGAC 
GAAGAAACAT TTGAGTTTAA GTTTAGTGGT GAGCTGACAT ACACCACTGT CCTGAGTGAT 
CAAAAGATGG TGGAGCTGGT TCCCGGAGGC AGCAACATAT CCGTGCTCTA TAAGGACCGT 
GTGGAATTTA TTAGAATGGT GCAGAAAGCA CGACTGGAGG AGAGTAAAGA ACAGATAGGA 
GCTCTCCAGG CTGGCCTTTT GAAGGTTGTC CCACAAGCAG TGCTAGATCT TCTTACATGG 
CAAGAACTTG AGAAAAGGGT TTGTGGGGAT CCAGAAATCA CTGTTGAAGC CCTAAAAAAA 
CTAACCAGGT TTGGGGACAT AGAACAAACA GACACTCGGG TCCAGTATTT CTGGGAGGCA 
CTAAACAATT TCACTAATGA GGACCGCAGC CGTTTCCTAA GGTTTGTGAC TGGCCGGAGT 
CGACTTCCAG CACCAATTTA TATCTACCCA GATAGATCAT GGCTGGGTAC AGACGACTCC 
CTACCTGAAT CATCCACCTG TTCTAGTTCC TTGTATCTGC CAAATTATGC CAGTGCAAAA 
ATTTGTGAAG AGAAACTGCG TTATGCTGCA TATAACTGTG TAGCCATCGA TACAGATATG 
AATCCATGGG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816779.1
CDS	306..2843
Translation	MQAPDNPHRLLGQIRCVLECIDCFRHVQPLPEALCYVPGEVQYKICKDTSSSSSSRSLLTVWEAPGYAKQNLKKLSRATVEVRKGSCVTATGEEYCNANGLWVKLSEAQMEEFKGSCDMAEGWVLLCRQDEGGDRLIPVESRDKTTRQQQVFGIDYKSVHMWEKVVDATYSMHLGAKPKIIKPDEEAVQKLRFLPPSWTYECDEDLVHFLFEHVVKQDESLGSVKQYVESISVSSATVENNLSCLTDGYHDTYWESDGSHGEHWIQLHMKEGTIVKRLLITVDSTDESYMPKGIVVYGGERDALKKINEVSLDENLIGDVCILEDMTTHLPIIEIKITQCLDEGIDVRIRGLKIKSSKRRDLGLTADLFQPNNLVRYPRLEAIEPDVLYHRAVVVQRFVKLLDNVLNHMVPAWDHSIGTFNQLKHIKQFLLLSKCRSSLITQCLKDSETMKPDFMPCLYINRRIAVEHRDNPSLDPSYKKTVFSQVYEGLKLTDKSEKPLDYRWPVRYEQWWECKFIAEGIIDQGGGFRDSLADISEELCPSSADIPVPLPFFVRTSNQGNGTGEAQDMYVPNPSCKDLAKYEWIGQLMGAALRGKEFLVLALPGLVWKQLTGEEVSWSKDFPAVDSLLVKLLEMMELMDEETFEFKFSGELTYTTVLSDQKMVELVPGGSNISVLYKDRVEFIRMVQKARLEESKEQIGALQAGLLKVVPQAVLDLLTWQELEKRVCGDPEITVEALKKLTRFGDIEQTDTRVQYFWEALNNFTNEDRSRFLRFVTGRSRLPAPIYIYPDRSWLGTDDSLPESSTCSSSLYLPNYASAKICEEKLRYAAYNCVAIDTDMNPWEE

Target ORF information:

RefSeq Version	XM_012961325.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961325.2

ORF Insert Sequence:

ATGCAGGCTC CAGATAATCC ACATCGACTA CTGGGCCAAA TCCGCTGTGT TTTGGAGTGT 
ATCGACTGCT TCCGACATGT GCAGCCCCTC CCCGAGGCCT TATGTTATGT TCCTGGGGAA 
GTGCAGTACA AAATCTGCAA GGACACCAGC TCATCATCAT CATCTCGTTC CCTGTTGACT 
GTTTGGGAGG CACCTGGATA TGCCAAGCAA AACTTGAAGA AATTGTCTCG GGCAACAGTT 
GAAGTGCGCA AAGGCAGCTG TGTCACAGCC ACAGGGGAGG AATACTGCAA TGCTAATGGA 
CTGTGGGTAA AGCTGAGCGA GGCACAAATG GAAGAATTTA AAGGTAGCTG TGACATGGCA 
GAGGGCTGGG TCTTGCTATG TCGGCAAGAT GAAGGAGGAG ACAGGCTTAT TCCTGTGGAG 
TCTAGAGATA AAACCACGAG GCAGCAACAG GTGTTTGGGA TAGACTACAA ATCAGTCCAC 
ATGTGGGAGA AGGTTGTTGA TGCAACTTAT TCTATGCACT TAGGGGCCAA ACCTAAAATC 
ATTAAGCCAG ATGAAGAGGC AGTTCAAAAA CTGCGCTTCC TTCCTCCATC TTGGACCTAT 
GAATGTGATG AAGATCTGGT GCACTTTCTT TTTGAGCACG TTGTAAAGCA AGATGAGAGC 
TTAGGAAGTG TGAAGCAGTA TGTAGAAAGC ATCAGTGTGT CCTCTGCTAC GGTGGAAAAC 
AACTTGTCGT GTTTGACAGA TGGTTACCAT GACACATATT GGGAGAGCGA TGGCTCCCAC 
GGTGAGCACT GGATCCAACT ACACATGAAA GAAGGCACCA TTGTCAAGAG ACTCCTGATA 
ACTGTGGACT CTACAGATGA ATCGTACATG CCCAAAGGGA TTGTGGTATA TGGAGGTGAG 
AGAGATGCCC TGAAAAAAAT CAACGAAGTC TCCTTAGACG AAAACCTGAT TGGAGATGTT 
TGTATCCTGG AAGATATGAC CACTCACCTA CCAATCATTG AGATCAAAAT CACACAGTGT 
CTGGATGAAG GAATTGATGT CCGGATCAGA GGGCTCAAGA TTAAATCTTC AAAAAGGCGT 
GATTTAGGCC TCACTGCTGA TCTTTTCCAG CCAAACAACT TAGTTCGGTA TCCACGCTTG 
GAGGCCATTG AGCCAGATGT GCTTTATCAT AGAGCTGTGG TAGTCCAGAG GTTTGTTAAG 
TTGCTGGATA ATGTGCTAAA TCACATGGTA CCGGCCTGGG ATCACAGCAT TGGCACATTC 
AATCAGCTAA AGCACATCAA ACAGTTCCTG CTGCTATCCA AATGCAGATC TTCACTTATT 
ACTCAGTGTC TAAAGGATTC AGAAACCATG AAACCCGACT TCATGCCCTG TTTATATATT 
AACCGTAGGA TTGCAGTGGA GCATCGGGAC AATCCTTCAT TGGACCCTTC ATACAAGAAG 
ACTGTTTTCA GTCAGGTATA TGAAGGCCTA AAGCTTACAG ATAAATCAGA GAAACCACTG 
GATTACAGGT GGCCTGTAAG GTATGAGCAA TGGTGGGAGT GTAAATTTAT TGCAGAAGGA 
ATTATTGATC AAGGGGGAGG TTTTAGGGAC AGTCTGGCTG ATATATCTGA GGAGCTTTGC 
CCAAGTTCTG CTGATATACC TGTTCCACTA CCATTTTTTG TACGCACTTC AAACCAGGGT 
AACGGAACTG GGGAAGCACA AGACATGTAT GTGCCAAATC CATCATGTAA GGACCTGGCA 
AAATATGAGT GGATTGGACA ACTTATGGGA GCAGCACTGA GAGGAAAGGA ATTCTTGGTT 
CTAGCTCTGC CAGGCCTGGT GTGGAAACAG TTGACTGGAG AAGAAGTAAG CTGGAGTAAG 
GATTTTCCTG CAGTGGATTC TTTACTTGTG AAACTTCTGG AGATGATGGA ACTTATGGAC 
GAAGAAACAT TTGAGTTTAA GTTTAGTGGT GAGCTGACAT ACACCACTGT CCTGAGTGAT 
CAAAAGATGG TGGAGCTGGT TCCCGGAGGC AGCAACATAT CCGTGCTCTA TAAGGACCGT 
GTGGAATTTA TTAGAATGGT GCAGAAAGCA CGACTGGAGG AGAGTAAAGA ACAGATAGGA 
GCTCTCCAGG CTGGCCTTTT GAAGGTTGTC CCACAAGCAG TGCTAGATCT TCTTACATGG 
CAAGAACTTG AGAAAAGGGT TTGTGGGGAT CCAGAAATCA CTGTTGAAGC CCTAAAAAAA 
CTAACCAGGT TTGGGGACAT AGAACAAACA GACACTCGGG TCCAGTATTT CTGGGAGGCA 
CTAAACAATT TCACTAATGA GGACCGCAGC CGTTTCCTAA GGTTTGTGAC TGGCCGGAGT 
CGACTTCCAG CACCAATTTA TATCTACCCA GATAGATCAT GGCTGGGTAC AGACGACTCC 
CTACCTGAAT CATCCACCTG TTCTAGTTCC TTGTATCTGC CAAATTATGC CAGTGCAAAA 
ATTTGTGAAG AGAAACTGCG TTATGCTGCA TATAACTGTG TAGCCATCGA TACAGATATG 
AATCCATGGG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd3 ( XM_012961326.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816780.1 Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), transcript variant X2, mRNA.

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CloneID	OXa29310
Clone ID Related Accession (Same CDS sequence)	XM_012961325.2 , XM_012961326.2
Accession Version	XM_012961326.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECTD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCTC CAGATAATCC ACATCGACTA CTGGGCCAAA TCCGCTGTGT TTTGGAGTGT 
ATCGACTGCT TCCGACATGT GCAGCCCCTC CCCGAGGCCT TATGTTATGT TCCTGGGGAA 
GTGCAGTACA AAATCTGCAA GGACACCAGC TCATCATCAT CATCTCGTTC CCTGTTGACT 
GTTTGGGAGG CACCTGGATA TGCCAAGCAA AACTTGAAGA AATTGTCTCG GGCAACAGTT 
GAAGTGCGCA AAGGCAGCTG TGTCACAGCC ACAGGGGAGG AATACTGCAA TGCTAATGGA 
CTGTGGGTAA AGCTGAGCGA GGCACAAATG GAAGAATTTA AAGGTAGCTG TGACATGGCA 
GAGGGCTGGG TCTTGCTATG TCGGCAAGAT GAAGGAGGAG ACAGGCTTAT TCCTGTGGAG 
TCTAGAGATA AAACCACGAG GCAGCAACAG GTGTTTGGGA TAGACTACAA ATCAGTCCAC 
ATGTGGGAGA AGGTTGTTGA TGCAACTTAT TCTATGCACT TAGGGGCCAA ACCTAAAATC 
ATTAAGCCAG ATGAAGAGGC AGTTCAAAAA CTGCGCTTCC TTCCTCCATC TTGGACCTAT 
GAATGTGATG AAGATCTGGT GCACTTTCTT TTTGAGCACG TTGTAAAGCA AGATGAGAGC 
TTAGGAAGTG TGAAGCAGTA TGTAGAAAGC ATCAGTGTGT CCTCTGCTAC GGTGGAAAAC 
AACTTGTCGT GTTTGACAGA TGGTTACCAT GACACATATT GGGAGAGCGA TGGCTCCCAC 
GGTGAGCACT GGATCCAACT ACACATGAAA GAAGGCACCA TTGTCAAGAG ACTCCTGATA 
ACTGTGGACT CTACAGATGA ATCGTACATG CCCAAAGGGA TTGTGGTATA TGGAGGTGAG 
AGAGATGCCC TGAAAAAAAT CAACGAAGTC TCCTTAGACG AAAACCTGAT TGGAGATGTT 
TGTATCCTGG AAGATATGAC CACTCACCTA CCAATCATTG AGATCAAAAT CACACAGTGT 
CTGGATGAAG GAATTGATGT CCGGATCAGA GGGCTCAAGA TTAAATCTTC AAAAAGGCGT 
GATTTAGGCC TCACTGCTGA TCTTTTCCAG CCAAACAACT TAGTTCGGTA TCCACGCTTG 
GAGGCCATTG AGCCAGATGT GCTTTATCAT AGAGCTGTGG TAGTCCAGAG GTTTGTTAAG 
TTGCTGGATA ATGTGCTAAA TCACATGGTA CCGGCCTGGG ATCACAGCAT TGGCACATTC 
AATCAGCTAA AGCACATCAA ACAGTTCCTG CTGCTATCCA AATGCAGATC TTCACTTATT 
ACTCAGTGTC TAAAGGATTC AGAAACCATG AAACCCGACT TCATGCCCTG TTTATATATT 
AACCGTAGGA TTGCAGTGGA GCATCGGGAC AATCCTTCAT TGGACCCTTC ATACAAGAAG 
ACTGTTTTCA GTCAGGTATA TGAAGGCCTA AAGCTTACAG ATAAATCAGA GAAACCACTG 
GATTACAGGT GGCCTGTAAG GTATGAGCAA TGGTGGGAGT GTAAATTTAT TGCAGAAGGA 
ATTATTGATC AAGGGGGAGG TTTTAGGGAC AGTCTGGCTG ATATATCTGA GGAGCTTTGC 
CCAAGTTCTG CTGATATACC TGTTCCACTA CCATTTTTTG TACGCACTTC AAACCAGGGT 
AACGGAACTG GGGAAGCACA AGACATGTAT GTGCCAAATC CATCATGTAA GGACCTGGCA 
AAATATGAGT GGATTGGACA ACTTATGGGA GCAGCACTGA GAGGAAAGGA ATTCTTGGTT 
CTAGCTCTGC CAGGCCTGGT GTGGAAACAG TTGACTGGAG AAGAAGTAAG CTGGAGTAAG 
GATTTTCCTG CAGTGGATTC TTTACTTGTG AAACTTCTGG AGATGATGGA ACTTATGGAC 
GAAGAAACAT TTGAGTTTAA GTTTAGTGGT GAGCTGACAT ACACCACTGT CCTGAGTGAT 
CAAAAGATGG TGGAGCTGGT TCCCGGAGGC AGCAACATAT CCGTGCTCTA TAAGGACCGT 
GTGGAATTTA TTAGAATGGT GCAGAAAGCA CGACTGGAGG AGAGTAAAGA ACAGATAGGA 
GCTCTCCAGG CTGGCCTTTT GAAGGTTGTC CCACAAGCAG TGCTAGATCT TCTTACATGG 
CAAGAACTTG AGAAAAGGGT TTGTGGGGAT CCAGAAATCA CTGTTGAAGC CCTAAAAAAA 
CTAACCAGGT TTGGGGACAT AGAACAAACA GACACTCGGG TCCAGTATTT CTGGGAGGCA 
CTAAACAATT TCACTAATGA GGACCGCAGC CGTTTCCTAA GGTTTGTGAC TGGCCGGAGT 
CGACTTCCAG CACCAATTTA TATCTACCCA GATAGATCAT GGCTGGGTAC AGACGACTCC 
CTACCTGAAT CATCCACCTG TTCTAGTTCC TTGTATCTGC CAAATTATGC CAGTGCAAAA 
ATTTGTGAAG AGAAACTGCG TTATGCTGCA TATAACTGTG TAGCCATCGA TACAGATATG 
AATCCATGGG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816780.1
CDS	132..2669
Translation	MQAPDNPHRLLGQIRCVLECIDCFRHVQPLPEALCYVPGEVQYKICKDTSSSSSSRSLLTVWEAPGYAKQNLKKLSRATVEVRKGSCVTATGEEYCNANGLWVKLSEAQMEEFKGSCDMAEGWVLLCRQDEGGDRLIPVESRDKTTRQQQVFGIDYKSVHMWEKVVDATYSMHLGAKPKIIKPDEEAVQKLRFLPPSWTYECDEDLVHFLFEHVVKQDESLGSVKQYVESISVSSATVENNLSCLTDGYHDTYWESDGSHGEHWIQLHMKEGTIVKRLLITVDSTDESYMPKGIVVYGGERDALKKINEVSLDENLIGDVCILEDMTTHLPIIEIKITQCLDEGIDVRIRGLKIKSSKRRDLGLTADLFQPNNLVRYPRLEAIEPDVLYHRAVVVQRFVKLLDNVLNHMVPAWDHSIGTFNQLKHIKQFLLLSKCRSSLITQCLKDSETMKPDFMPCLYINRRIAVEHRDNPSLDPSYKKTVFSQVYEGLKLTDKSEKPLDYRWPVRYEQWWECKFIAEGIIDQGGGFRDSLADISEELCPSSADIPVPLPFFVRTSNQGNGTGEAQDMYVPNPSCKDLAKYEWIGQLMGAALRGKEFLVLALPGLVWKQLTGEEVSWSKDFPAVDSLLVKLLEMMELMDEETFEFKFSGELTYTTVLSDQKMVELVPGGSNISVLYKDRVEFIRMVQKARLEESKEQIGALQAGLLKVVPQAVLDLLTWQELEKRVCGDPEITVEALKKLTRFGDIEQTDTRVQYFWEALNNFTNEDRSRFLRFVTGRSRLPAPIYIYPDRSWLGTDDSLPESSTCSSSLYLPNYASAKICEEKLRYAAYNCVAIDTDMNPWEE

Target ORF information:

RefSeq Version	XM_012961326.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961326.2

ORF Insert Sequence:

ATGCAGGCTC CAGATAATCC ACATCGACTA CTGGGCCAAA TCCGCTGTGT TTTGGAGTGT 
ATCGACTGCT TCCGACATGT GCAGCCCCTC CCCGAGGCCT TATGTTATGT TCCTGGGGAA 
GTGCAGTACA AAATCTGCAA GGACACCAGC TCATCATCAT CATCTCGTTC CCTGTTGACT 
GTTTGGGAGG CACCTGGATA TGCCAAGCAA AACTTGAAGA AATTGTCTCG GGCAACAGTT 
GAAGTGCGCA AAGGCAGCTG TGTCACAGCC ACAGGGGAGG AATACTGCAA TGCTAATGGA 
CTGTGGGTAA AGCTGAGCGA GGCACAAATG GAAGAATTTA AAGGTAGCTG TGACATGGCA 
GAGGGCTGGG TCTTGCTATG TCGGCAAGAT GAAGGAGGAG ACAGGCTTAT TCCTGTGGAG 
TCTAGAGATA AAACCACGAG GCAGCAACAG GTGTTTGGGA TAGACTACAA ATCAGTCCAC 
ATGTGGGAGA AGGTTGTTGA TGCAACTTAT TCTATGCACT TAGGGGCCAA ACCTAAAATC 
ATTAAGCCAG ATGAAGAGGC AGTTCAAAAA CTGCGCTTCC TTCCTCCATC TTGGACCTAT 
GAATGTGATG AAGATCTGGT GCACTTTCTT TTTGAGCACG TTGTAAAGCA AGATGAGAGC 
TTAGGAAGTG TGAAGCAGTA TGTAGAAAGC ATCAGTGTGT CCTCTGCTAC GGTGGAAAAC 
AACTTGTCGT GTTTGACAGA TGGTTACCAT GACACATATT GGGAGAGCGA TGGCTCCCAC 
GGTGAGCACT GGATCCAACT ACACATGAAA GAAGGCACCA TTGTCAAGAG ACTCCTGATA 
ACTGTGGACT CTACAGATGA ATCGTACATG CCCAAAGGGA TTGTGGTATA TGGAGGTGAG 
AGAGATGCCC TGAAAAAAAT CAACGAAGTC TCCTTAGACG AAAACCTGAT TGGAGATGTT 
TGTATCCTGG AAGATATGAC CACTCACCTA CCAATCATTG AGATCAAAAT CACACAGTGT 
CTGGATGAAG GAATTGATGT CCGGATCAGA GGGCTCAAGA TTAAATCTTC AAAAAGGCGT 
GATTTAGGCC TCACTGCTGA TCTTTTCCAG CCAAACAACT TAGTTCGGTA TCCACGCTTG 
GAGGCCATTG AGCCAGATGT GCTTTATCAT AGAGCTGTGG TAGTCCAGAG GTTTGTTAAG 
TTGCTGGATA ATGTGCTAAA TCACATGGTA CCGGCCTGGG ATCACAGCAT TGGCACATTC 
AATCAGCTAA AGCACATCAA ACAGTTCCTG CTGCTATCCA AATGCAGATC TTCACTTATT 
ACTCAGTGTC TAAAGGATTC AGAAACCATG AAACCCGACT TCATGCCCTG TTTATATATT 
AACCGTAGGA TTGCAGTGGA GCATCGGGAC AATCCTTCAT TGGACCCTTC ATACAAGAAG 
ACTGTTTTCA GTCAGGTATA TGAAGGCCTA AAGCTTACAG ATAAATCAGA GAAACCACTG 
GATTACAGGT GGCCTGTAAG GTATGAGCAA TGGTGGGAGT GTAAATTTAT TGCAGAAGGA 
ATTATTGATC AAGGGGGAGG TTTTAGGGAC AGTCTGGCTG ATATATCTGA GGAGCTTTGC 
CCAAGTTCTG CTGATATACC TGTTCCACTA CCATTTTTTG TACGCACTTC AAACCAGGGT 
AACGGAACTG GGGAAGCACA AGACATGTAT GTGCCAAATC CATCATGTAA GGACCTGGCA 
AAATATGAGT GGATTGGACA ACTTATGGGA GCAGCACTGA GAGGAAAGGA ATTCTTGGTT 
CTAGCTCTGC CAGGCCTGGT GTGGAAACAG TTGACTGGAG AAGAAGTAAG CTGGAGTAAG 
GATTTTCCTG CAGTGGATTC TTTACTTGTG AAACTTCTGG AGATGATGGA ACTTATGGAC 
GAAGAAACAT TTGAGTTTAA GTTTAGTGGT GAGCTGACAT ACACCACTGT CCTGAGTGAT 
CAAAAGATGG TGGAGCTGGT TCCCGGAGGC AGCAACATAT CCGTGCTCTA TAAGGACCGT 
GTGGAATTTA TTAGAATGGT GCAGAAAGCA CGACTGGAGG AGAGTAAAGA ACAGATAGGA 
GCTCTCCAGG CTGGCCTTTT GAAGGTTGTC CCACAAGCAG TGCTAGATCT TCTTACATGG 
CAAGAACTTG AGAAAAGGGT TTGTGGGGAT CCAGAAATCA CTGTTGAAGC CCTAAAAAAA 
CTAACCAGGT TTGGGGACAT AGAACAAACA GACACTCGGG TCCAGTATTT CTGGGAGGCA 
CTAAACAATT TCACTAATGA GGACCGCAGC CGTTTCCTAA GGTTTGTGAC TGGCCGGAGT 
CGACTTCCAG CACCAATTTA TATCTACCCA GATAGATCAT GGCTGGGTAC AGACGACTCC 
CTACCTGAAT CATCCACCTG TTCTAGTTCC TTGTATCTGC CAAATTATGC CAGTGCAAAA 
ATTTGTGAAG AGAAACTGCG TTATGCTGCA TATAACTGTG TAGCCATCGA TACAGATATG 
AATCCATGGG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd3 ( XM_012961326.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816780.2 Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa56159
Clone ID Related Accession (Same CDS sequence)	XM_012961326.3 , XM_012961325.3
Accession Version	XM_012961326.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECTD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961326.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCTC CAGATAATCC ACATCGACTA CTGGGCCAAA TCCGCTGTGT TTTGGAGTGT 
ATCGACTGCT TCCGACATGT GCAGCCCCTC CCCGAGGCCT TATGTTATGT TCCTGGGGAA 
GTGCAGTACA AAATCTGCAA GGACACCAGC TCATCATCAT CATCTCGTTC CCTGTTGACT 
GTTTGGGAGG CACCTGGATA TGCCAAGCAA AACTTGAAGA AATTGTCTCG GGCAACAGTT 
GAAGTGCGCA AAGGCAGCTG TGTCACAGCC ACAGGGGAGG AATACTGCAA TGCTAATGGA 
CTGTGGGTAA AGCTGAGCGA GGCACAAATG GAAGAATTTA AAGGTAGCTG TGACATGGCA 
GAGGGCTGGG TCTTGCTATG TCGGCAAGAT GAAGGAGGAG ACAGGCTTAT TCCTGTGGAG 
TCTACAGATA AAACCACGAG GCAGCAACAG GTGTTTGGGA TAGACTACAA ATCAGTCCAC 
ATGTGGGAGA AGGTTGTTGA TGCAACTTAT TCTATGCACT TAGGGGCCAA ACCTAAAATC 
ATTAAGCCAG ATGAAGAGGC AGTTCAAAAA CTGCGCTTCC TTCCTCCATC TTGGACCTAT 
GAATGTGATG AAGATCTGGT GCACTTTCTT TTTGAGCACG TTGTAAAGCA AGATGAGAGC 
TTAGGAAGTG TGAAGCAGTA TGTAGAAAGC ATCAGTGTGT CCTCTGCTAC GGTGGAAAAC 
AACTTGTCGT GTTTGACAGA TGGTTACCAT GACACATATT GGGAGAGCGA TGGCTCCCAC 
GGTGAGCACT GGATCCAACT ACACATGAAA GAAGGCACCA TTGTCAAGAG ACTCCTGATA 
ACTGTGGACT CTACAGATGA ATCGTACATG CCCAAAGGGA TTGTGGTATA TGGAGGTGAG 
AGAGATGCCC TGAAAAAAAT CAACGAAGTC TCCTTAGACG AAAACCTGAT TGGAGATGTT 
TGTATCCTGG AAGATATGAC CACTCACCTA CCAATCATTG AGATCAAAAT CACACAGTGT 
CTGGATGAAG GAATTGATGT CCGGATCAGA GGGCTCAAGA TTAAATCTTC AAAAAGGCGT 
GATTTAGGCC TCACTGCTGA TCTTTTCCAG CCAAACAACT TAGTTCGGTA TCCACGCTTG 
GAGGCCATTG AGCCAGATGT GCTTTATCAT AGAGCTGTGG TAGTCCAGAG GTTTGTTAAG 
TTGCTGGATA ATGTGCTAAA TCACATGGTA CCGGCCTGGG ATCACAGCAT TGGCACATTC 
AATCAGCTAA AGCACATCAA ACAGTTCCTG CTGCTATCCA AATGCAGATC TTCACTTATT 
ACTCAGTGTC TAAAGGATTC AGAAACCATG AAACCCGACT TCATGCCCTG TTTATATATT 
AACCGTAGGA TTGCAGTGGA GCATCGGGAC AATCCTTCAT TGGACCCTTC ATACAAGAAG 
ACTGTTTTCA GTCAGGTATA TGAAGGCCTA AAGCTTACAG ATAAATCAGA GAAACCACTG 
GATTACAGGT GGCCTGTAAG GTATGAGCAA TGGTGGGAGT GTAAATTTAT TGCAGAAGGA 
ATTATTGATC AAGGGGGAGG TTTTAGGGAC AGTCTGGCTG ATATATCTGA GGAGCTTTGC 
CCAAGTTCTG CTGATATACC TGTTCCACTA CCATTTTTTG TACGCACTTC AAACCAGGGT 
AACGGAACTG GGGAAGCACA AGACATGTAT GTGCCAAATC CATCATGTAA GGACCTGGCA 
AAATATGAGT GGATTGGACA ACTTATGGGA GCAGCACTGA GAGGAAAGGA ATTCTTGGTT 
CTAGCTCTGC CAGGCCTGGT GTGGAAACAG TTGACTGGAG AAGAAGTAAG CTGGAGTAAG 
GATTTTCCTG CAGTGGATTC TTTACTTGTG AAACTTCTGG AGATGATGGA ACTTATGGAC 
GAAGAAACAT TTGAGTTTAA GTTTAGTGGT GAGCTGACAT ACACCACTGT CCTGAGTGAT 
CAAAAGATGG TGGAGCTGGT TCCCGGAGGC AGCAACATAT CCGTGCTCTA TAAGGACCGT 
GTGGAATTTA TTAGAATGGT GCAGAAAGCA CGACTGGAGG AGAGTAAAGA ACAGATAGGA 
GCTCTCCAGG CTGGCCTTTT GAAGGTTGTC CCACAAGCAG TGCTAGATCT TCTTACATGG 
CAAGAACTTG AGAAAAGGGT TTGTGGGGAT CCAGAAATCA CTGTTGAAGC CCTAAAAAAA 
CTAACCAGGT TTGGGGACAT AGAACAAACA GACACTCGGG TCCAGTATTT CTGGGAGGCA 
CTAAACAATT TCACTAATGA GGACCGCAGC CGTTTCCTAA GGTTTGTGAC TGGCCGGAGT 
CGACTTCCAG CACCAATTTA TATCTACCCA GATAGATCAT GGCTGGGTAC AGACGACTCC 
CTACCTGAAT CATCCACCTG TTCTAGTTCC TTGTATCTGC CAAATTATGC CAGTGCAAAA 
ATTTGTGAAG AGAAACTGCG TTATGCTGCA TATAACTGTG TAGCCATCGA TACAGATATG 
AATCCATGGG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816780.2
CDS	134..2671
Translation	MQAPDNPHRLLGQIRCVLECIDCFRHVQPLPEALCYVPGEVQYKICKDTSSSSSSRSLLTVWEAPGYAKQNLKKLSRATVEVRKGSCVTATGEEYCNANGLWVKLSEAQMEEFKGSCDMAEGWVLLCRQDEGGDRLIPVESTDKTTRQQQVFGIDYKSVHMWEKVVDATYSMHLGAKPKIIKPDEEAVQKLRFLPPSWTYECDEDLVHFLFEHVVKQDESLGSVKQYVESISVSSATVENNLSCLTDGYHDTYWESDGSHGEHWIQLHMKEGTIVKRLLITVDSTDESYMPKGIVVYGGERDALKKINEVSLDENLIGDVCILEDMTTHLPIIEIKITQCLDEGIDVRIRGLKIKSSKRRDLGLTADLFQPNNLVRYPRLEAIEPDVLYHRAVVVQRFVKLLDNVLNHMVPAWDHSIGTFNQLKHIKQFLLLSKCRSSLITQCLKDSETMKPDFMPCLYINRRIAVEHRDNPSLDPSYKKTVFSQVYEGLKLTDKSEKPLDYRWPVRYEQWWECKFIAEGIIDQGGGFRDSLADISEELCPSSADIPVPLPFFVRTSNQGNGTGEAQDMYVPNPSCKDLAKYEWIGQLMGAALRGKEFLVLALPGLVWKQLTGEEVSWSKDFPAVDSLLVKLLEMMELMDEETFEFKFSGELTYTTVLSDQKMVELVPGGSNISVLYKDRVEFIRMVQKARLEESKEQIGALQAGLLKVVPQAVLDLLTWQELEKRVCGDPEITVEALKKLTRFGDIEQTDTRVQYFWEALNNFTNEDRSRFLRFVTGRSRLPAPIYIYPDRSWLGTDDSLPESSTCSSSLYLPNYASAKICEEKLRYAAYNCVAIDTDMNPWEE

Target ORF information:

RefSeq Version	XM_012961326.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961326.3

ORF Insert Sequence:

ATGCAGGCTC CAGATAATCC ACATCGACTA CTGGGCCAAA TCCGCTGTGT TTTGGAGTGT 
ATCGACTGCT TCCGACATGT GCAGCCCCTC CCCGAGGCCT TATGTTATGT TCCTGGGGAA 
GTGCAGTACA AAATCTGCAA GGACACCAGC TCATCATCAT CATCTCGTTC CCTGTTGACT 
GTTTGGGAGG CACCTGGATA TGCCAAGCAA AACTTGAAGA AATTGTCTCG GGCAACAGTT 
GAAGTGCGCA AAGGCAGCTG TGTCACAGCC ACAGGGGAGG AATACTGCAA TGCTAATGGA 
CTGTGGGTAA AGCTGAGCGA GGCACAAATG GAAGAATTTA AAGGTAGCTG TGACATGGCA 
GAGGGCTGGG TCTTGCTATG TCGGCAAGAT GAAGGAGGAG ACAGGCTTAT TCCTGTGGAG 
TCTACAGATA AAACCACGAG GCAGCAACAG GTGTTTGGGA TAGACTACAA ATCAGTCCAC 
ATGTGGGAGA AGGTTGTTGA TGCAACTTAT TCTATGCACT TAGGGGCCAA ACCTAAAATC 
ATTAAGCCAG ATGAAGAGGC AGTTCAAAAA CTGCGCTTCC TTCCTCCATC TTGGACCTAT 
GAATGTGATG AAGATCTGGT GCACTTTCTT TTTGAGCACG TTGTAAAGCA AGATGAGAGC 
TTAGGAAGTG TGAAGCAGTA TGTAGAAAGC ATCAGTGTGT CCTCTGCTAC GGTGGAAAAC 
AACTTGTCGT GTTTGACAGA TGGTTACCAT GACACATATT GGGAGAGCGA TGGCTCCCAC 
GGTGAGCACT GGATCCAACT ACACATGAAA GAAGGCACCA TTGTCAAGAG ACTCCTGATA 
ACTGTGGACT CTACAGATGA ATCGTACATG CCCAAAGGGA TTGTGGTATA TGGAGGTGAG 
AGAGATGCCC TGAAAAAAAT CAACGAAGTC TCCTTAGACG AAAACCTGAT TGGAGATGTT 
TGTATCCTGG AAGATATGAC CACTCACCTA CCAATCATTG AGATCAAAAT CACACAGTGT 
CTGGATGAAG GAATTGATGT CCGGATCAGA GGGCTCAAGA TTAAATCTTC AAAAAGGCGT 
GATTTAGGCC TCACTGCTGA TCTTTTCCAG CCAAACAACT TAGTTCGGTA TCCACGCTTG 
GAGGCCATTG AGCCAGATGT GCTTTATCAT AGAGCTGTGG TAGTCCAGAG GTTTGTTAAG 
TTGCTGGATA ATGTGCTAAA TCACATGGTA CCGGCCTGGG ATCACAGCAT TGGCACATTC 
AATCAGCTAA AGCACATCAA ACAGTTCCTG CTGCTATCCA AATGCAGATC TTCACTTATT 
ACTCAGTGTC TAAAGGATTC AGAAACCATG AAACCCGACT TCATGCCCTG TTTATATATT 
AACCGTAGGA TTGCAGTGGA GCATCGGGAC AATCCTTCAT TGGACCCTTC ATACAAGAAG 
ACTGTTTTCA GTCAGGTATA TGAAGGCCTA AAGCTTACAG ATAAATCAGA GAAACCACTG 
GATTACAGGT GGCCTGTAAG GTATGAGCAA TGGTGGGAGT GTAAATTTAT TGCAGAAGGA 
ATTATTGATC AAGGGGGAGG TTTTAGGGAC AGTCTGGCTG ATATATCTGA GGAGCTTTGC 
CCAAGTTCTG CTGATATACC TGTTCCACTA CCATTTTTTG TACGCACTTC AAACCAGGGT 
AACGGAACTG GGGAAGCACA AGACATGTAT GTGCCAAATC CATCATGTAA GGACCTGGCA 
AAATATGAGT GGATTGGACA ACTTATGGGA GCAGCACTGA GAGGAAAGGA ATTCTTGGTT 
CTAGCTCTGC CAGGCCTGGT GTGGAAACAG TTGACTGGAG AAGAAGTAAG CTGGAGTAAG 
GATTTTCCTG CAGTGGATTC TTTACTTGTG AAACTTCTGG AGATGATGGA ACTTATGGAC 
GAAGAAACAT TTGAGTTTAA GTTTAGTGGT GAGCTGACAT ACACCACTGT CCTGAGTGAT 
CAAAAGATGG TGGAGCTGGT TCCCGGAGGC AGCAACATAT CCGTGCTCTA TAAGGACCGT 
GTGGAATTTA TTAGAATGGT GCAGAAAGCA CGACTGGAGG AGAGTAAAGA ACAGATAGGA 
GCTCTCCAGG CTGGCCTTTT GAAGGTTGTC CCACAAGCAG TGCTAGATCT TCTTACATGG 
CAAGAACTTG AGAAAAGGGT TTGTGGGGAT CCAGAAATCA CTGTTGAAGC CCTAAAAAAA 
CTAACCAGGT TTGGGGACAT AGAACAAACA GACACTCGGG TCCAGTATTT CTGGGAGGCA 
CTAAACAATT TCACTAATGA GGACCGCAGC CGTTTCCTAA GGTTTGTGAC TGGCCGGAGT 
CGACTTCCAG CACCAATTTA TATCTACCCA GATAGATCAT GGCTGGGTAC AGACGACTCC 
CTACCTGAAT CATCCACCTG TTCTAGTTCC TTGTATCTGC CAAATTATGC CAGTGCAAAA 
ATTTGTGAAG AGAAACTGCG TTATGCTGCA TATAACTGTG TAGCCATCGA TACAGATATG 
AATCCATGGG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd3 ( XM_012961325.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816779.2 Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), transcript variant X1, mRNA.

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CloneID	OXa56159
Clone ID Related Accession (Same CDS sequence)	XM_012961326.3 , XM_012961325.3
Accession Version	XM_012961325.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECTD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961325.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCTC CAGATAATCC ACATCGACTA CTGGGCCAAA TCCGCTGTGT TTTGGAGTGT 
ATCGACTGCT TCCGACATGT GCAGCCCCTC CCCGAGGCCT TATGTTATGT TCCTGGGGAA 
GTGCAGTACA AAATCTGCAA GGACACCAGC TCATCATCAT CATCTCGTTC CCTGTTGACT 
GTTTGGGAGG CACCTGGATA TGCCAAGCAA AACTTGAAGA AATTGTCTCG GGCAACAGTT 
GAAGTGCGCA AAGGCAGCTG TGTCACAGCC ACAGGGGAGG AATACTGCAA TGCTAATGGA 
CTGTGGGTAA AGCTGAGCGA GGCACAAATG GAAGAATTTA AAGGTAGCTG TGACATGGCA 
GAGGGCTGGG TCTTGCTATG TCGGCAAGAT GAAGGAGGAG ACAGGCTTAT TCCTGTGGAG 
TCTACAGATA AAACCACGAG GCAGCAACAG GTGTTTGGGA TAGACTACAA ATCAGTCCAC 
ATGTGGGAGA AGGTTGTTGA TGCAACTTAT TCTATGCACT TAGGGGCCAA ACCTAAAATC 
ATTAAGCCAG ATGAAGAGGC AGTTCAAAAA CTGCGCTTCC TTCCTCCATC TTGGACCTAT 
GAATGTGATG AAGATCTGGT GCACTTTCTT TTTGAGCACG TTGTAAAGCA AGATGAGAGC 
TTAGGAAGTG TGAAGCAGTA TGTAGAAAGC ATCAGTGTGT CCTCTGCTAC GGTGGAAAAC 
AACTTGTCGT GTTTGACAGA TGGTTACCAT GACACATATT GGGAGAGCGA TGGCTCCCAC 
GGTGAGCACT GGATCCAACT ACACATGAAA GAAGGCACCA TTGTCAAGAG ACTCCTGATA 
ACTGTGGACT CTACAGATGA ATCGTACATG CCCAAAGGGA TTGTGGTATA TGGAGGTGAG 
AGAGATGCCC TGAAAAAAAT CAACGAAGTC TCCTTAGACG AAAACCTGAT TGGAGATGTT 
TGTATCCTGG AAGATATGAC CACTCACCTA CCAATCATTG AGATCAAAAT CACACAGTGT 
CTGGATGAAG GAATTGATGT CCGGATCAGA GGGCTCAAGA TTAAATCTTC AAAAAGGCGT 
GATTTAGGCC TCACTGCTGA TCTTTTCCAG CCAAACAACT TAGTTCGGTA TCCACGCTTG 
GAGGCCATTG AGCCAGATGT GCTTTATCAT AGAGCTGTGG TAGTCCAGAG GTTTGTTAAG 
TTGCTGGATA ATGTGCTAAA TCACATGGTA CCGGCCTGGG ATCACAGCAT TGGCACATTC 
AATCAGCTAA AGCACATCAA ACAGTTCCTG CTGCTATCCA AATGCAGATC TTCACTTATT 
ACTCAGTGTC TAAAGGATTC AGAAACCATG AAACCCGACT TCATGCCCTG TTTATATATT 
AACCGTAGGA TTGCAGTGGA GCATCGGGAC AATCCTTCAT TGGACCCTTC ATACAAGAAG 
ACTGTTTTCA GTCAGGTATA TGAAGGCCTA AAGCTTACAG ATAAATCAGA GAAACCACTG 
GATTACAGGT GGCCTGTAAG GTATGAGCAA TGGTGGGAGT GTAAATTTAT TGCAGAAGGA 
ATTATTGATC AAGGGGGAGG TTTTAGGGAC AGTCTGGCTG ATATATCTGA GGAGCTTTGC 
CCAAGTTCTG CTGATATACC TGTTCCACTA CCATTTTTTG TACGCACTTC AAACCAGGGT 
AACGGAACTG GGGAAGCACA AGACATGTAT GTGCCAAATC CATCATGTAA GGACCTGGCA 
AAATATGAGT GGATTGGACA ACTTATGGGA GCAGCACTGA GAGGAAAGGA ATTCTTGGTT 
CTAGCTCTGC CAGGCCTGGT GTGGAAACAG TTGACTGGAG AAGAAGTAAG CTGGAGTAAG 
GATTTTCCTG CAGTGGATTC TTTACTTGTG AAACTTCTGG AGATGATGGA ACTTATGGAC 
GAAGAAACAT TTGAGTTTAA GTTTAGTGGT GAGCTGACAT ACACCACTGT CCTGAGTGAT 
CAAAAGATGG TGGAGCTGGT TCCCGGAGGC AGCAACATAT CCGTGCTCTA TAAGGACCGT 
GTGGAATTTA TTAGAATGGT GCAGAAAGCA CGACTGGAGG AGAGTAAAGA ACAGATAGGA 
GCTCTCCAGG CTGGCCTTTT GAAGGTTGTC CCACAAGCAG TGCTAGATCT TCTTACATGG 
CAAGAACTTG AGAAAAGGGT TTGTGGGGAT CCAGAAATCA CTGTTGAAGC CCTAAAAAAA 
CTAACCAGGT TTGGGGACAT AGAACAAACA GACACTCGGG TCCAGTATTT CTGGGAGGCA 
CTAAACAATT TCACTAATGA GGACCGCAGC CGTTTCCTAA GGTTTGTGAC TGGCCGGAGT 
CGACTTCCAG CACCAATTTA TATCTACCCA GATAGATCAT GGCTGGGTAC AGACGACTCC 
CTACCTGAAT CATCCACCTG TTCTAGTTCC TTGTATCTGC CAAATTATGC CAGTGCAAAA 
ATTTGTGAAG AGAAACTGCG TTATGCTGCA TATAACTGTG TAGCCATCGA TACAGATATG 
AATCCATGGG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816779.2
CDS	306..2843
Translation	MQAPDNPHRLLGQIRCVLECIDCFRHVQPLPEALCYVPGEVQYKICKDTSSSSSSRSLLTVWEAPGYAKQNLKKLSRATVEVRKGSCVTATGEEYCNANGLWVKLSEAQMEEFKGSCDMAEGWVLLCRQDEGGDRLIPVESTDKTTRQQQVFGIDYKSVHMWEKVVDATYSMHLGAKPKIIKPDEEAVQKLRFLPPSWTYECDEDLVHFLFEHVVKQDESLGSVKQYVESISVSSATVENNLSCLTDGYHDTYWESDGSHGEHWIQLHMKEGTIVKRLLITVDSTDESYMPKGIVVYGGERDALKKINEVSLDENLIGDVCILEDMTTHLPIIEIKITQCLDEGIDVRIRGLKIKSSKRRDLGLTADLFQPNNLVRYPRLEAIEPDVLYHRAVVVQRFVKLLDNVLNHMVPAWDHSIGTFNQLKHIKQFLLLSKCRSSLITQCLKDSETMKPDFMPCLYINRRIAVEHRDNPSLDPSYKKTVFSQVYEGLKLTDKSEKPLDYRWPVRYEQWWECKFIAEGIIDQGGGFRDSLADISEELCPSSADIPVPLPFFVRTSNQGNGTGEAQDMYVPNPSCKDLAKYEWIGQLMGAALRGKEFLVLALPGLVWKQLTGEEVSWSKDFPAVDSLLVKLLEMMELMDEETFEFKFSGELTYTTVLSDQKMVELVPGGSNISVLYKDRVEFIRMVQKARLEESKEQIGALQAGLLKVVPQAVLDLLTWQELEKRVCGDPEITVEALKKLTRFGDIEQTDTRVQYFWEALNNFTNEDRSRFLRFVTGRSRLPAPIYIYPDRSWLGTDDSLPESSTCSSSLYLPNYASAKICEEKLRYAAYNCVAIDTDMNPWEE

Target ORF information:

RefSeq Version	XM_012961325.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 3 (hectd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961325.3

ORF Insert Sequence:

ATGCAGGCTC CAGATAATCC ACATCGACTA CTGGGCCAAA TCCGCTGTGT TTTGGAGTGT 
ATCGACTGCT TCCGACATGT GCAGCCCCTC CCCGAGGCCT TATGTTATGT TCCTGGGGAA 
GTGCAGTACA AAATCTGCAA GGACACCAGC TCATCATCAT CATCTCGTTC CCTGTTGACT 
GTTTGGGAGG CACCTGGATA TGCCAAGCAA AACTTGAAGA AATTGTCTCG GGCAACAGTT 
GAAGTGCGCA AAGGCAGCTG TGTCACAGCC ACAGGGGAGG AATACTGCAA TGCTAATGGA 
CTGTGGGTAA AGCTGAGCGA GGCACAAATG GAAGAATTTA AAGGTAGCTG TGACATGGCA 
GAGGGCTGGG TCTTGCTATG TCGGCAAGAT GAAGGAGGAG ACAGGCTTAT TCCTGTGGAG 
TCTACAGATA AAACCACGAG GCAGCAACAG GTGTTTGGGA TAGACTACAA ATCAGTCCAC 
ATGTGGGAGA AGGTTGTTGA TGCAACTTAT TCTATGCACT TAGGGGCCAA ACCTAAAATC 
ATTAAGCCAG ATGAAGAGGC AGTTCAAAAA CTGCGCTTCC TTCCTCCATC TTGGACCTAT 
GAATGTGATG AAGATCTGGT GCACTTTCTT TTTGAGCACG TTGTAAAGCA AGATGAGAGC 
TTAGGAAGTG TGAAGCAGTA TGTAGAAAGC ATCAGTGTGT CCTCTGCTAC GGTGGAAAAC 
AACTTGTCGT GTTTGACAGA TGGTTACCAT GACACATATT GGGAGAGCGA TGGCTCCCAC 
GGTGAGCACT GGATCCAACT ACACATGAAA GAAGGCACCA TTGTCAAGAG ACTCCTGATA 
ACTGTGGACT CTACAGATGA ATCGTACATG CCCAAAGGGA TTGTGGTATA TGGAGGTGAG 
AGAGATGCCC TGAAAAAAAT CAACGAAGTC TCCTTAGACG AAAACCTGAT TGGAGATGTT 
TGTATCCTGG AAGATATGAC CACTCACCTA CCAATCATTG AGATCAAAAT CACACAGTGT 
CTGGATGAAG GAATTGATGT CCGGATCAGA GGGCTCAAGA TTAAATCTTC AAAAAGGCGT 
GATTTAGGCC TCACTGCTGA TCTTTTCCAG CCAAACAACT TAGTTCGGTA TCCACGCTTG 
GAGGCCATTG AGCCAGATGT GCTTTATCAT AGAGCTGTGG TAGTCCAGAG GTTTGTTAAG 
TTGCTGGATA ATGTGCTAAA TCACATGGTA CCGGCCTGGG ATCACAGCAT TGGCACATTC 
AATCAGCTAA AGCACATCAA ACAGTTCCTG CTGCTATCCA AATGCAGATC TTCACTTATT 
ACTCAGTGTC TAAAGGATTC AGAAACCATG AAACCCGACT TCATGCCCTG TTTATATATT 
AACCGTAGGA TTGCAGTGGA GCATCGGGAC AATCCTTCAT TGGACCCTTC ATACAAGAAG 
ACTGTTTTCA GTCAGGTATA TGAAGGCCTA AAGCTTACAG ATAAATCAGA GAAACCACTG 
GATTACAGGT GGCCTGTAAG GTATGAGCAA TGGTGGGAGT GTAAATTTAT TGCAGAAGGA 
ATTATTGATC AAGGGGGAGG TTTTAGGGAC AGTCTGGCTG ATATATCTGA GGAGCTTTGC 
CCAAGTTCTG CTGATATACC TGTTCCACTA CCATTTTTTG TACGCACTTC AAACCAGGGT 
AACGGAACTG GGGAAGCACA AGACATGTAT GTGCCAAATC CATCATGTAA GGACCTGGCA 
AAATATGAGT GGATTGGACA ACTTATGGGA GCAGCACTGA GAGGAAAGGA ATTCTTGGTT 
CTAGCTCTGC CAGGCCTGGT GTGGAAACAG TTGACTGGAG AAGAAGTAAG CTGGAGTAAG 
GATTTTCCTG CAGTGGATTC TTTACTTGTG AAACTTCTGG AGATGATGGA ACTTATGGAC 
GAAGAAACAT TTGAGTTTAA GTTTAGTGGT GAGCTGACAT ACACCACTGT CCTGAGTGAT 
CAAAAGATGG TGGAGCTGGT TCCCGGAGGC AGCAACATAT CCGTGCTCTA TAAGGACCGT 
GTGGAATTTA TTAGAATGGT GCAGAAAGCA CGACTGGAGG AGAGTAAAGA ACAGATAGGA 
GCTCTCCAGG CTGGCCTTTT GAAGGTTGTC CCACAAGCAG TGCTAGATCT TCTTACATGG 
CAAGAACTTG AGAAAAGGGT TTGTGGGGAT CCAGAAATCA CTGTTGAAGC CCTAAAAAAA 
CTAACCAGGT TTGGGGACAT AGAACAAACA GACACTCGGG TCCAGTATTT CTGGGAGGCA 
CTAAACAATT TCACTAATGA GGACCGCAGC CGTTTCCTAA GGTTTGTGAC TGGCCGGAGT 
CGACTTCCAG CACCAATTTA TATCTACCCA GATAGATCAT GGCTGGGTAC AGACGACTCC 
CTACCTGAAT CATCCACCTG TTCTAGTTCC TTGTATCTGC CAAATTATGC CAGTGCAAAA 
ATTTGTGAAG AGAAACTGCG TTATGCTGCA TATAACTGTG TAGCCATCGA TACAGATATG 
AATCCATGGG AAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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hectd3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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