EFL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EFL1
Entrez Gene ID	79631
Full Name	elongation factor like GTPase 1
Synonyms	EFTUD1,FAM42A,HsT19294,RIA1
General protein information	Preferred Names elongation factor like GTPase 1 Names elongation factor-like GTPase 1 elongation factor Tu GTP binding domain containing 1 elongation factor Tu GTP-binding domain-containing protein 1 elongation factor-like 1 ribosome assembly 1 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q25.2
Disorder MIM:	617538

mRNA and Protein(s)

mRNA	Protein	Name
NM_024580.6	NP_078856.4	elongation factor-like GTPase 1 isoform 1
NM_001322844.1	NP_001309773.1	elongation factor-like GTPase 1 isoform 3
NM_001322845.1	NP_001309774.1	elongation factor-like GTPase 1 isoform 1
XM_024450048.1	XP_024305816.1	elongation factor-like GTPase 1 isoform X2
NM_024580.5	NP_078856.4	elongation factor-like GTPase 1 isoform 1
XM_011522000.1	XP_011520302.1	elongation factor-like GTPase 1 isoform X1
NM_001040610.2	NP_001035700.1	elongation factor-like GTPase 1 isoform 2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG33158	NP_788515.1
Schizosaccharomyces pombe (fission yeast)	SPCC553.08c	NP_587766.1
Magnaporthe oryzae (rice blast fungus)	MGG_05265	XP_003712823.1
Arabidopsis thaliana (thale cress)	AT3G22980	NP_188938.1
Oryza sativa (rice)	Os03g0650700	NP_001050786.1
Bos taurus (cattle)	EFTUD1	XP_002696652.1
Danio rerio (zebrafish)	eftud1	XP_697184.5
Saccharomyces cerevisiae (baker's yeast)	RIA1	NP_014236.1
Eremothecium gossypii	AGOS_AFR031C	NP_985578.2
Pan troglodytes (chimpanzee)	EFTUD1	XP_510546.2
	EFTUD1	XP_002804960.1
Mus musculus (house mouse)	Eftud1	NP_780526.2
Rattus norvegicus (Norway rat)	Eftud1	NP_001101004.1
Gallus gallus (chicken)	EFTUD1	NP_001034345.2
Homo sapiens (human)	EFTUD1	NP_078856.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003001	XP_311881.3
Kluyveromyces lactis	KLLA0D07898g	XP_453415.1
Oryza sativa (rice)	Os02g0543300	NP_001047074.1
Canis lupus familiaris (dog)	EFTUD1	XP_850111.1
Neurospora crassa	NCU06278	XP_962882.2
Xenopus tropicalis (tropical clawed frog)	eftud1	XP_002940146.2

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in
Stepensky P, Chac?n-Flores M, Kim KH, Abuzaitoun O, Bautista-Santos A, Simanovsky N, Siliqi D, Altamura D, M?ndez-Godoy A, Gijsbers A, Naser Eddin A, Dor T, Charrow J, S?nchez-Puig N, Elpeleg O
Journal of medical genetics54(8)558-566(2017 Aug)

Mechanism of eIF6 release from the nascent 60S ribosomal subunit.
Weis F, Giudice E, Churcher M, Jin L, Hilcenko C, Wong CC, Traynor D, Kay RR, Warren AJ
Nature structural & molecular biology22(11)914-9(2015 Nov)

Defective Guanine Nucleotide Exchange in the Elongation Factor-like 1 (EFL1) GTPase by Mutations in the Shwachman-Diamond Syndrome Protein.
Garc?a-M?rquez A, Gijsbers A, de la Mora E, S?nchez-Puig N
The Journal of biological chemistry290(29)17669-78(2015 Jul)

Functional analysis of a novel glioma antigen, EFTUD1.
Saito K, Iizuka Y, Ohta S, Takahashi S, Nakamura K, Saya H, Yoshida K, Kawakami Y, Toda M
Neuro-oncology16(12)1618-29(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Upon EFL1 binding, SBDS is repositioned around helix 69, thus facilitating a conformational switch in EFL1 that displaces eIF6 by competing for an overlapping binding site on the 60S ribosomal subunit.
Title: Mechanism of eIF6 release from the nascent 60S ribosomal subunit.

Association of Elongation Factor-like 1 (EFL1) GTPase to SBDS did not modify the affinity for GTP but dramatically decreased that for GDP by increasing the dissociation rate of the nucleotide.
Title: Defective Guanine Nucleotide Exchange in the Elongation Factor-like 1 (EFL1) GTPase by Mutations in the Shwachman-Diamond Syndrome Protein.

Downregulation of EFTUD1 induced cell-cycle arrest and apoptosis in gliomas by impairing ribosome biogenesis
Title: Functional analysis of a novel glioma antigen, EFTUD1.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genomewide linkage and peakwide association analyses of carotid plaque in Caribbean Hispanics.

The following EFL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu71951	NM_001322844.1 Latest version!	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu71951	XM_011522000.1 Latest version!	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu104058	XM_024450048.1 Latest version!	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu04111	NM_001322845.1 Latest version!	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu04111	NM_024580.6 Latest version!	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu04996	NM_001040610.2 Latest version!	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu04111	NM_024580.5	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EFL1 ( NM_001322844.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309773.1 Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu71951
Clone ID Related Accession (Same CDS sequence)	NM_001322844.1 , XM_011522000.1
Accession Version	NM_001322844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	elongation factor-like GTPase 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC026624.8 and AC026956.20. On Apr 13, 2016 this sequence version replaced XM_011521999.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.331370.1, SRR1803616.168231.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAAAACCT TGTGGGGAGA TTACTATATA AATATGAAGG CTAAAAAGAT CATGAAGGGT 
GATCAGGCCA AAGGAAAGAA ACCTTTATTT GTACAGTTGA TCCTGGAAAA TATATGGAGT 
TTGTATGATG CTGTTTTGAA AAAGGACAAA GACAAAATTG ATAAAATAGT GACTTCTTTA 
GGATTAAAAA TTGGAGCCCG GGAGGCACGA CATTCAGACC CTAAAGTTCA GATCAACGCC 
ATTTGCAGTC AGTGGCTACC CATATCCCAT GCTGTTCTTG CTATGGTGTG TCAGAAACTT 
CCTAGTCCCC TTGATATTAC AGCTGAGAGA GTGGAGAGAC TGATGTGCAC AGGATCACAA 
ACTTTTGACT CTTTTCCACC AGAAACTCAA GCACTGAAAG CAGCTTTTAT GAAATGTGGA 
AGTGAGGACA CTGCTCCAGT TATTATATTT GTTTCCAAAA TGTTTGCAGT TGATGCTAAG 
GCCTTGCCTC AGAATAAGCC AAGGCCTCTC ACTCAAGAAG AAATTGCTCA GAGACGTGAG 
CGTGCAAGAC AAAGGCATGC AGAGAAGCTT GCAGCAGCAC AGGGACAGGC ACCCTTGGAG 
CCCACCCAAG ATGGGAGTGC CATTGAAACA TGTCCAAAAG GAGAGGAGCC AAGAGGTGAC 
GAGCAACAGG TGGAAAGTAT GACCCCTAAA CCTGTGCTCC AGGAAGAAAA CAACCAAGAG 
TCTTTTATTG CATTTGCTCG GGTGTTCAGT GGTGTGGCTC GAAGAGGAAA GAAAATTTTT 
GTCTTGGGGC CCAAATACAG TCCTCTTGAG TTTTTACGAA GGGTACCATT AGGCTTCTCA 
GCTCCACCAG ATGGCCTCCC CCAAGTCCCC CACATGGCAT ACTGTGCTCT GGAAAACCTG 
TATCTTCTGA TGGGAAGGGA ACTGGAATAT CTAGAGGAGG TACCTCCAGG AAATGTGCTA 
GGAATAGGAG GCCTTCAAGA TTTTGTGCTG AAATCTGCAA CACTGTGTAG CCTGCCATCC 
TGCCCACCAT TTATACCACT CAACTTCGAA GCCACTCCTA TTGTGAGAGT TGCTGTTGAA 
CCAAAACATC CAAGTGAAAT GCCTCAGCTC GTAAAAGGAA TGAAACTGTT AAACCAGGCT 
GATCCCTGTG TCCAGATTTT AATTCAGGAA ACGGGAGAGC ACGTTTTAGT CACAGCAGGA 
GAAGTCCACC TTCAGCGATG CCTGGATGAC TTAAAAGAAA GGTTTGCAAA GATTCATATC 
AGTGTATCTG AACCTATTAT TCCATTCAGA GAAACAATCA CAAAACCCCC AAAAGTTGAC 
ATGGTCAATG AAGAAATAGG CAAACAGCAA AAAGTTGCAG TCATACACCA AATGAAAGAA 
GATCAAAGCA AAATCCCTGA AGGAATCCAA GTTGACTCTG ACGGGCTAAT CACCATAACA 
ACTCCCAATA AACTTGCCAC GCTCAGTGTT CGAGCCATGC CCCTTCCAGA AGAAGTCACC 
CAGATTCTGG AAGAAAATAG TGATTTGATT CGTTCTATGG AGCAGTTGAC ATCCTCTTTG 
AATGAGGGTG AAAATACTCA CATGATTCAT CAGAAGACCC AAGAGAAAAT TTGGGAATTC 
AAAGGAAAAC TGGAGCAACA CCTAACAGGG AGAAGATGGA GGAACATTGT TGACCAAATC 
TGGTCATTTG GCCCAAGAAA ATGTGGGCCC AACATACTAG TCAATAAAAG TGAAGATTTT 
CAGAACTCAG TATGGACAGG TCCAGCTGAC AAAGCTTCAA AAGAAGCCAG TAGATACCGA 
GATTTGGGCA ATAGCATTGT GAGTGGCTTC CAACTAGCAA CCCTCTCTGG CCCCATGTGT 
GAGGAGCCTC TCATGGGTGT CTGTTTTGTT CTGGAAAAAT GGGACCTAAG TAAATTTGAG 
GAACAAGGAG CAAGTGATCT GGCAAAAGAG GGACAGGAGG AAAATGAAAC CTGTTCTGGT 
GGAAATGAAA ACCAAGAGCT ACAAGATGGC TGCTCTGAGG CCTTTGAGAA GAGGACATCA 
CAGAAAGGAG AATCTCCACT CACTGACTGC TATGGACCTT TCTCAGGACA GCTAATTGCC 
ACCATGAAAG AAGCATGTCG CTATGCACTG CAAGTGAAAC CTCAGCGCCT GATGGCAGCT 
ATGTACACAT GTGACATCAT GGCCACTGGT GATGTTCTCG GTCGAGTCTA TGCTGTCTTG 
TCAAAGAGAG AAGGTCGGGT ACTTCAAGAA GAAATGAAAG AAGGGACAGA CATGTTCATC 
ATCAAGGCTG TGCTGCCTGT TGCTGAAAGC TTTGGTTTTG CTGATGAAAT CAGGAAGAGG 
ACAAGTGGCC TGGCCAGCCC ACAACTAGTA TTCAGCCATT GGGAGATCAT TCCCAGTGAC 
CCCTTCTGGG TGCCAACTAC TGAGGAGGAA TACTTGCACT TTGGGGAGAA GGCTGACTCT 
GAGAACCAAG CCCGGAAGTA CATGAACGCA GTACGAAAGC GGAAGGGGCT TTATGTGGAA 
GAAAAGATTG TGGAGCATGC AGAAAAGCAG AGGACACTCA GCAAAAATAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309773.1
CDS	874..3447
Translation	MKTLWGDYYINMKAKKIMKGDQAKGKKPLFVQLILENIWSLYDAVLKKDKDKIDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISHAVLAMVCQKLPSPLDITAERVERLMCTGSQTFDSFPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAQRRERARQRHAEKLAAAQGQAPLEPTQDGSAIETCPKGEEPRGDEQQVESMTPKPVLQEENNQESFIAFARVFSGVARRGKKIFVLGPKYSPLEFLRRVPLGFSAPPDGLPQVPHMAYCALENLYLLMGRELEYLEEVPPGNVLGIGGLQDFVLKSATLCSLPSCPPFIPLNFEATPIVRVAVEPKHPSEMPQLVKGMKLLNQADPCVQILIQETGEHVLVTAGEVHLQRCLDDLKERFAKIHISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQMKEDQSKIPEGIQVDSDGLITITTPNKLATLSVRAMPLPEEVTQILEENSDLIRSMEQLTSSLNEGENTHMIHQKTQEKIWEFKGKLEQHLTGRRWRNIVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPADKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDLAKEGQEENETCSGGNENQELQDGCSEAFEKRTSQKGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	NM_001322844.1
Organism	Homo sapiens(human)
Definition	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322844.1

ORF Insert Sequence:

ATGAAAACCT TGTGGGGAGA TTACTATATA AATATGAAGG CTAAAAAGAT CATGAAGGGT 
GATCAGGCCA AAGGAAAGAA ACCTTTATTT GTACAGTTGA TCCTGGAAAA TATATGGAGT 
TTGTATGATG CTGTTTTGAA AAAGGACAAA GACAAAATTG ATAAAATAGT GACTTCTTTA 
GGATTAAAAA TTGGAGCCCG GGAGGCACGA CATTCAGACC CTAAAGTTCA GATCAACGCC 
ATTTGCAGTC AGTGGCTACC CATATCCCAT GCTGTTCTTG CTATGGTGTG TCAGAAACTT 
CCTAGTCCCC TTGATATTAC AGCTGAGAGA GTGGAGAGAC TGATGTGCAC AGGATCACAA 
ACTTTTGACT CTTTTCCACC AGAAACTCAA GCACTGAAAG CAGCTTTTAT GAAATGTGGA 
AGTGAGGACA CTGCTCCAGT TATTATATTT GTTTCCAAAA TGTTTGCAGT TGATGCTAAG 
GCCTTGCCTC AGAATAAGCC AAGGCCTCTC ACTCAAGAAG AAATTGCTCA GAGACGTGAG 
CGTGCAAGAC AAAGGCATGC AGAGAAGCTT GCAGCAGCAC AGGGACAGGC ACCCTTGGAG 
CCCACCCAAG ATGGGAGTGC CATTGAAACA TGTCCAAAAG GAGAGGAGCC AAGAGGTGAC 
GAGCAACAGG TGGAAAGTAT GACCCCTAAA CCTGTGCTCC AGGAAGAAAA CAACCAAGAG 
TCTTTTATTG CATTTGCTCG GGTGTTCAGT GGTGTGGCTC GAAGAGGAAA GAAAATTTTT 
GTCTTGGGGC CCAAATACAG TCCTCTTGAG TTTTTACGAA GGGTACCATT AGGCTTCTCA 
GCTCCACCAG ATGGCCTCCC CCAAGTCCCC CACATGGCAT ACTGTGCTCT GGAAAACCTG 
TATCTTCTGA TGGGAAGGGA ACTGGAATAT CTAGAGGAGG TACCTCCAGG AAATGTGCTA 
GGAATAGGAG GCCTTCAAGA TTTTGTGCTG AAATCTGCAA CACTGTGTAG CCTGCCATCC 
TGCCCACCAT TTATACCACT CAACTTCGAA GCCACTCCTA TTGTGAGAGT TGCTGTTGAA 
CCAAAACATC CAAGTGAAAT GCCTCAGCTC GTAAAAGGAA TGAAACTGTT AAACCAGGCT 
GATCCCTGTG TCCAGATTTT AATTCAGGAA ACGGGAGAGC ACGTTTTAGT CACAGCAGGA 
GAAGTCCACC TTCAGCGATG CCTGGATGAC TTAAAAGAAA GGTTTGCAAA GATTCATATC 
AGTGTATCTG AACCTATTAT TCCATTCAGA GAAACAATCA CAAAACCCCC AAAAGTTGAC 
ATGGTCAATG AAGAAATAGG CAAACAGCAA AAAGTTGCAG TCATACACCA AATGAAAGAA 
GATCAAAGCA AAATCCCTGA AGGAATCCAA GTTGACTCTG ACGGGCTAAT CACCATAACA 
ACTCCCAATA AACTTGCCAC GCTCAGTGTT CGAGCCATGC CCCTTCCAGA AGAAGTCACC 
CAGATTCTGG AAGAAAATAG TGATTTGATT CGTTCTATGG AGCAGTTGAC ATCCTCTTTG 
AATGAGGGTG AAAATACTCA CATGATTCAT CAGAAGACCC AAGAGAAAAT TTGGGAATTC 
AAAGGAAAAC TGGAGCAACA CCTAACAGGG AGAAGATGGA GGAACATTGT TGACCAAATC 
TGGTCATTTG GCCCAAGAAA ATGTGGGCCC AACATACTAG TCAATAAAAG TGAAGATTTT 
CAGAACTCAG TATGGACAGG TCCAGCTGAC AAAGCTTCAA AAGAAGCCAG TAGATACCGA 
GATTTGGGCA ATAGCATTGT GAGTGGCTTC CAACTAGCAA CCCTCTCTGG CCCCATGTGT 
GAGGAGCCTC TCATGGGTGT CTGTTTTGTT CTGGAAAAAT GGGACCTAAG TAAATTTGAG 
GAACAAGGAG CAAGTGATCT GGCAAAAGAG GGACAGGAGG AAAATGAAAC CTGTTCTGGT 
GGAAATGAAA ACCAAGAGCT ACAAGATGGC TGCTCTGAGG CCTTTGAGAA GAGGACATCA 
CAGAAAGGAG AATCTCCACT CACTGACTGC TATGGACCTT TCTCAGGACA GCTAATTGCC 
ACCATGAAAG AAGCATGTCG CTATGCACTG CAAGTGAAAC CTCAGCGCCT GATGGCAGCT 
ATGTACACAT GTGACATCAT GGCCACTGGT GATGTTCTCG GTCGAGTCTA TGCTGTCTTG 
TCAAAGAGAG AAGGTCGGGT ACTTCAAGAA GAAATGAAAG AAGGGACAGA CATGTTCATC 
ATCAAGGCTG TGCTGCCTGT TGCTGAAAGC TTTGGTTTTG CTGATGAAAT CAGGAAGAGG 
ACAAGTGGCC TGGCCAGCCC ACAACTAGTA TTCAGCCATT GGGAGATCAT TCCCAGTGAC 
CCCTTCTGGG TGCCAACTAC TGAGGAGGAA TACTTGCACT TTGGGGAGAA GGCTGACTCT 
GAGAACCAAG CCCGGAAGTA CATGAACGCA GTACGAAAGC GGAAGGGGCT TTATGTGGAA 
GAAAAGATTG TGGAGCATGC AGAAAAGCAG AGGACACTCA GCAAAAATAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( XM_011522000.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520302.1 Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu71951
Clone ID Related Accession (Same CDS sequence)	NM_001322844.1 , XM_011522000.1
Accession Version	XM_011522000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	elongation factor-like GTPase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACCT TGTGGGGAGA TTACTATATA AATATGAAGG CTAAAAAGAT CATGAAGGGT 
GATCAGGCCA AAGGAAAGAA ACCTTTATTT GTACAGTTGA TCCTGGAAAA TATATGGAGT 
TTGTATGATG CTGTTTTGAA AAAGGACAAA GACAAAATTG ATAAAATAGT GACTTCTTTA 
GGATTAAAAA TTGGAGCCCG GGAGGCACGA CATTCAGACC CTAAAGTTCA GATCAACGCC 
ATTTGCAGTC AGTGGCTACC CATATCCCAT GCTGTTCTTG CTATGGTGTG TCAGAAACTT 
CCTAGTCCCC TTGATATTAC AGCTGAGAGA GTGGAGAGAC TGATGTGCAC AGGATCACAA 
ACTTTTGACT CTTTTCCACC AGAAACTCAA GCACTGAAAG CAGCTTTTAT GAAATGTGGA 
AGTGAGGACA CTGCTCCAGT TATTATATTT GTTTCCAAAA TGTTTGCAGT TGATGCTAAG 
GCCTTGCCTC AGAATAAGCC AAGGCCTCTC ACTCAAGAAG AAATTGCTCA GAGACGTGAG 
CGTGCAAGAC AAAGGCATGC AGAGAAGCTT GCAGCAGCAC AGGGACAGGC ACCCTTGGAG 
CCCACCCAAG ATGGGAGTGC CATTGAAACA TGTCCAAAAG GAGAGGAGCC AAGAGGTGAC 
GAGCAACAGG TGGAAAGTAT GACCCCTAAA CCTGTGCTCC AGGAAGAAAA CAACCAAGAG 
TCTTTTATTG CATTTGCTCG GGTGTTCAGT GGTGTGGCTC GAAGAGGAAA GAAAATTTTT 
GTCTTGGGGC CCAAATACAG TCCTCTTGAG TTTTTACGAA GGGTACCATT AGGCTTCTCA 
GCTCCACCAG ATGGCCTCCC CCAAGTCCCC CACATGGCAT ACTGTGCTCT GGAAAACCTG 
TATCTTCTGA TGGGAAGGGA ACTGGAATAT CTAGAGGAGG TACCTCCAGG AAATGTGCTA 
GGAATAGGAG GCCTTCAAGA TTTTGTGCTG AAATCTGCAA CACTGTGTAG CCTGCCATCC 
TGCCCACCAT TTATACCACT CAACTTCGAA GCCACTCCTA TTGTGAGAGT TGCTGTTGAA 
CCAAAACATC CAAGTGAAAT GCCTCAGCTC GTAAAAGGAA TGAAACTGTT AAACCAGGCT 
GATCCCTGTG TCCAGATTTT AATTCAGGAA ACGGGAGAGC ACGTTTTAGT CACAGCAGGA 
GAAGTCCACC TTCAGCGATG CCTGGATGAC TTAAAAGAAA GGTTTGCAAA GATTCATATC 
AGTGTATCTG AACCTATTAT TCCATTCAGA GAAACAATCA CAAAACCCCC AAAAGTTGAC 
ATGGTCAATG AAGAAATAGG CAAACAGCAA AAAGTTGCAG TCATACACCA AATGAAAGAA 
GATCAAAGCA AAATCCCTGA AGGAATCCAA GTTGACTCTG ACGGGCTAAT CACCATAACA 
ACTCCCAATA AACTTGCCAC GCTCAGTGTT CGAGCCATGC CCCTTCCAGA AGAAGTCACC 
CAGATTCTGG AAGAAAATAG TGATTTGATT CGTTCTATGG AGCAGTTGAC ATCCTCTTTG 
AATGAGGGTG AAAATACTCA CATGATTCAT CAGAAGACCC AAGAGAAAAT TTGGGAATTC 
AAAGGAAAAC TGGAGCAACA CCTAACAGGG AGAAGATGGA GGAACATTGT TGACCAAATC 
TGGTCATTTG GCCCAAGAAA ATGTGGGCCC AACATACTAG TCAATAAAAG TGAAGATTTT 
CAGAACTCAG TATGGACAGG TCCAGCTGAC AAAGCTTCAA AAGAAGCCAG TAGATACCGA 
GATTTGGGCA ATAGCATTGT GAGTGGCTTC CAACTAGCAA CCCTCTCTGG CCCCATGTGT 
GAGGAGCCTC TCATGGGTGT CTGTTTTGTT CTGGAAAAAT GGGACCTAAG TAAATTTGAG 
GAACAAGGAG CAAGTGATCT GGCAAAAGAG GGACAGGAGG AAAATGAAAC CTGTTCTGGT 
GGAAATGAAA ACCAAGAGCT ACAAGATGGC TGCTCTGAGG CCTTTGAGAA GAGGACATCA 
CAGAAAGGAG AATCTCCACT CACTGACTGC TATGGACCTT TCTCAGGACA GCTAATTGCC 
ACCATGAAAG AAGCATGTCG CTATGCACTG CAAGTGAAAC CTCAGCGCCT GATGGCAGCT 
ATGTACACAT GTGACATCAT GGCCACTGGT GATGTTCTCG GTCGAGTCTA TGCTGTCTTG 
TCAAAGAGAG AAGGTCGGGT ACTTCAAGAA GAAATGAAAG AAGGGACAGA CATGTTCATC 
ATCAAGGCTG TGCTGCCTGT TGCTGAAAGC TTTGGTTTTG CTGATGAAAT CAGGAAGAGG 
ACAAGTGGCC TGGCCAGCCC ACAACTAGTA TTCAGCCATT GGGAGATCAT TCCCAGTGAC 
CCCTTCTGGG TGCCAACTAC TGAGGAGGAA TACTTGCACT TTGGGGAGAA GGCTGACTCT 
GAGAACCAAG CCCGGAAGTA CATGAACGCA GTACGAAAGC GGAAGGGGCT TTATGTGGAA 
GAAAAGATTG TGGAGCATGC AGAAAAGCAG AGGACACTCA GCAAAAATAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520302.1
CDS	630..3203
Translation	MKTLWGDYYINMKAKKIMKGDQAKGKKPLFVQLILENIWSLYDAVLKKDKDKIDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISHAVLAMVCQKLPSPLDITAERVERLMCTGSQTFDSFPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAQRRERARQRHAEKLAAAQGQAPLEPTQDGSAIETCPKGEEPRGDEQQVESMTPKPVLQEENNQESFIAFARVFSGVARRGKKIFVLGPKYSPLEFLRRVPLGFSAPPDGLPQVPHMAYCALENLYLLMGRELEYLEEVPPGNVLGIGGLQDFVLKSATLCSLPSCPPFIPLNFEATPIVRVAVEPKHPSEMPQLVKGMKLLNQADPCVQILIQETGEHVLVTAGEVHLQRCLDDLKERFAKIHISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQMKEDQSKIPEGIQVDSDGLITITTPNKLATLSVRAMPLPEEVTQILEENSDLIRSMEQLTSSLNEGENTHMIHQKTQEKIWEFKGKLEQHLTGRRWRNIVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPADKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDLAKEGQEENETCSGGNENQELQDGCSEAFEKRTSQKGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	XM_011522000.1
Organism	Homo sapiens(human)
Definition	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522000.1

ORF Insert Sequence:

ATGAAAACCT TGTGGGGAGA TTACTATATA AATATGAAGG CTAAAAAGAT CATGAAGGGT 
GATCAGGCCA AAGGAAAGAA ACCTTTATTT GTACAGTTGA TCCTGGAAAA TATATGGAGT 
TTGTATGATG CTGTTTTGAA AAAGGACAAA GACAAAATTG ATAAAATAGT GACTTCTTTA 
GGATTAAAAA TTGGAGCCCG GGAGGCACGA CATTCAGACC CTAAAGTTCA GATCAACGCC 
ATTTGCAGTC AGTGGCTACC CATATCCCAT GCTGTTCTTG CTATGGTGTG TCAGAAACTT 
CCTAGTCCCC TTGATATTAC AGCTGAGAGA GTGGAGAGAC TGATGTGCAC AGGATCACAA 
ACTTTTGACT CTTTTCCACC AGAAACTCAA GCACTGAAAG CAGCTTTTAT GAAATGTGGA 
AGTGAGGACA CTGCTCCAGT TATTATATTT GTTTCCAAAA TGTTTGCAGT TGATGCTAAG 
GCCTTGCCTC AGAATAAGCC AAGGCCTCTC ACTCAAGAAG AAATTGCTCA GAGACGTGAG 
CGTGCAAGAC AAAGGCATGC AGAGAAGCTT GCAGCAGCAC AGGGACAGGC ACCCTTGGAG 
CCCACCCAAG ATGGGAGTGC CATTGAAACA TGTCCAAAAG GAGAGGAGCC AAGAGGTGAC 
GAGCAACAGG TGGAAAGTAT GACCCCTAAA CCTGTGCTCC AGGAAGAAAA CAACCAAGAG 
TCTTTTATTG CATTTGCTCG GGTGTTCAGT GGTGTGGCTC GAAGAGGAAA GAAAATTTTT 
GTCTTGGGGC CCAAATACAG TCCTCTTGAG TTTTTACGAA GGGTACCATT AGGCTTCTCA 
GCTCCACCAG ATGGCCTCCC CCAAGTCCCC CACATGGCAT ACTGTGCTCT GGAAAACCTG 
TATCTTCTGA TGGGAAGGGA ACTGGAATAT CTAGAGGAGG TACCTCCAGG AAATGTGCTA 
GGAATAGGAG GCCTTCAAGA TTTTGTGCTG AAATCTGCAA CACTGTGTAG CCTGCCATCC 
TGCCCACCAT TTATACCACT CAACTTCGAA GCCACTCCTA TTGTGAGAGT TGCTGTTGAA 
CCAAAACATC CAAGTGAAAT GCCTCAGCTC GTAAAAGGAA TGAAACTGTT AAACCAGGCT 
GATCCCTGTG TCCAGATTTT AATTCAGGAA ACGGGAGAGC ACGTTTTAGT CACAGCAGGA 
GAAGTCCACC TTCAGCGATG CCTGGATGAC TTAAAAGAAA GGTTTGCAAA GATTCATATC 
AGTGTATCTG AACCTATTAT TCCATTCAGA GAAACAATCA CAAAACCCCC AAAAGTTGAC 
ATGGTCAATG AAGAAATAGG CAAACAGCAA AAAGTTGCAG TCATACACCA AATGAAAGAA 
GATCAAAGCA AAATCCCTGA AGGAATCCAA GTTGACTCTG ACGGGCTAAT CACCATAACA 
ACTCCCAATA AACTTGCCAC GCTCAGTGTT CGAGCCATGC CCCTTCCAGA AGAAGTCACC 
CAGATTCTGG AAGAAAATAG TGATTTGATT CGTTCTATGG AGCAGTTGAC ATCCTCTTTG 
AATGAGGGTG AAAATACTCA CATGATTCAT CAGAAGACCC AAGAGAAAAT TTGGGAATTC 
AAAGGAAAAC TGGAGCAACA CCTAACAGGG AGAAGATGGA GGAACATTGT TGACCAAATC 
TGGTCATTTG GCCCAAGAAA ATGTGGGCCC AACATACTAG TCAATAAAAG TGAAGATTTT 
CAGAACTCAG TATGGACAGG TCCAGCTGAC AAAGCTTCAA AAGAAGCCAG TAGATACCGA 
GATTTGGGCA ATAGCATTGT GAGTGGCTTC CAACTAGCAA CCCTCTCTGG CCCCATGTGT 
GAGGAGCCTC TCATGGGTGT CTGTTTTGTT CTGGAAAAAT GGGACCTAAG TAAATTTGAG 
GAACAAGGAG CAAGTGATCT GGCAAAAGAG GGACAGGAGG AAAATGAAAC CTGTTCTGGT 
GGAAATGAAA ACCAAGAGCT ACAAGATGGC TGCTCTGAGG CCTTTGAGAA GAGGACATCA 
CAGAAAGGAG AATCTCCACT CACTGACTGC TATGGACCTT TCTCAGGACA GCTAATTGCC 
ACCATGAAAG AAGCATGTCG CTATGCACTG CAAGTGAAAC CTCAGCGCCT GATGGCAGCT 
ATGTACACAT GTGACATCAT GGCCACTGGT GATGTTCTCG GTCGAGTCTA TGCTGTCTTG 
TCAAAGAGAG AAGGTCGGGT ACTTCAAGAA GAAATGAAAG AAGGGACAGA CATGTTCATC 
ATCAAGGCTG TGCTGCCTGT TGCTGAAAGC TTTGGTTTTG CTGATGAAAT CAGGAAGAGG 
ACAAGTGGCC TGGCCAGCCC ACAACTAGTA TTCAGCCATT GGGAGATCAT TCCCAGTGAC 
CCCTTCTGGG TGCCAACTAC TGAGGAGGAA TACTTGCACT TTGGGGAGAA GGCTGACTCT 
GAGAACCAAG CCCGGAAGTA CATGAACGCA GTACGAAAGC GGAAGGGGCT TTATGTGGAA 
GAAAAGATTG TGGAGCATGC AGAAAAGCAG AGGACACTCA GCAAAAATAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( XM_024450048.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305816.1 Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant X2, mRNA.

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CloneID	OHu104058
Clone ID Related Accession (Same CDS sequence)	XM_024450048.1
Accession Version	XM_024450048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	elongation factor-like GTPase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTACCAT TAGGCTTCTC AGCTCCACCA GATGGCCTCC CCCAAGTCCC CCACATGGCA 
TACTGTGCTC TGGAAAACCT GTATCTTCTG ATGGGAAGGG AACTGGAATA TCTAGAGGAG 
GTACCTCCAG GAAATGTGCT AGGAATAGGA GGCCTTCAAG ATTTTGTGCT GAAATCTGCA 
ACACTGTGTA GCCTGCCATC CTGCCCACCA TTTATACCAC TCAACTTCGA AGCCACTCCT 
ATTGTGAGAG TTGCTGTTGA ACCAAAACAT CCAAGTGAAA TGCCTCAGCT CGTAAAAGGA 
ATGAAACTGT TAAACCAGGC TGATCCCTGT GTCCAGATTT TAATTCAGGA AACGGGAGAG 
CACGTTTTAG TCACAGCAGG AGAAGTCCAC CTTCAGCGAT GCCTGGATGA CTTAAAAGAA 
AGGTTTGCAA AGATTCATAT CAGTGTATCT GAACCTATTA TTCCATTCAG AGAAACAATC 
ACAAAACCCC CAAAAGTTGA CATGGTCAAT GAAGAAATAG GCAAACAGCA AAAAGTTGCA 
GTCATACACC AAATGAAAGA AGATCAAAGC AAAATCCCTG AAGGAATCCA AGTTGACTCT 
GACGGGCTAA TCACCATAAC AACTCCCAAT AAACTTGCCA CGCTCAGTGT TCGAGCCATG 
CCCCTTCCAG AAGAAGTCAC CCAGATTCTG GAAGAAAATA GTGATTTGAT TCGTTCTATG 
GAGCAGTTGA CATCCTCTTT GAATGAGGGT GAAAATACTC ACATGATTCA TCAGAAGACC 
CAAGAGAAAA TTTGGGAATT CAAAGGAAAA CTGGAGCAAC ACCTAACAGG GAGAAGATGG 
AGGAACATTG TTGACCAAAT CTGGTCATTT GGCCCAAGAA AATGTGGGCC CAACATACTA 
GTCAATAAAA GTGAAGATTT TCAGAACTCA GTATGGACAG GTCCAGCTGA CAAAGCTTCA 
AAAGAAGCCA GTAGATACCG AGATTTGGGC AATAGCATTG TGAGTGGCTT CCAACTAGCA 
ACCCTCTCTG GCCCCATGTG TGAGGAGCCT CTCATGGGTG TCTGTTTTGT TCTGGAAAAA 
TGGGACCTAA GTAAATTTGA GGAACAAGGA GCAAGTGATC TGGCAAAAGA GGGACAGGAG 
GAAAATGAAA CCTGTTCTGG TGGAAATGAA AACCAAGAGC TACAAGATGG CTGCTCTGAG 
GCCTTTGAGA AGAGGACATC ACAGAAAGGA GAATCTCCAC TCACTGACTG CTATGGACCT 
TTCTCAGGAC AGCTAATTGC CACCATGAAA GAAGCATGTC GCTATGCACT GCAAGTGAAA 
CCTCAGCGCC TGATGGCAGC TATGTACACA TGTGACATCA TGGCCACTGG TGATGTTCTC 
GGTCGAGTCT ATGCTGTCTT GTCAAAGAGA GAAGGTCGGG TACTTCAAGA AGAAATGAAA 
GAAGGGACAG ACATGTTCAT CATCAAGGCT GTGCTGCCTG TTGCTGAAAG CTTTGGTTTT 
GCTGATGAAA TCAGGAAGAG GACAAGTGGC CTGGCCAGCC CACAACTAGT ATTCAGCCAT 
TGGGAGATCA TTCCCAGTGA CCCCTTCTGG GTGCCAACTA CTGAGGAGGA ATACTTGCAC 
TTTGGGGAGA AGGCTGACTC TGAGAACCAA GCCCGGAAGT ACATGAACGC AGTACGAAAG 
CGGAAGGGGC TTTATGTGGA AGAAAAGATT GTGGAGCATG CAGAAAAGCA GAGGACACTC 
AGCAAAAATA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305816.1
CDS	293..2047
Translation	MVPLGFSAPPDGLPQVPHMAYCALENLYLLMGRELEYLEEVPPGNVLGIGGLQDFVLKSATLCSLPSCPPFIPLNFEATPIVRVAVEPKHPSEMPQLVKGMKLLNQADPCVQILIQETGEHVLVTAGEVHLQRCLDDLKERFAKIHISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQMKEDQSKIPEGIQVDSDGLITITTPNKLATLSVRAMPLPEEVTQILEENSDLIRSMEQLTSSLNEGENTHMIHQKTQEKIWEFKGKLEQHLTGRRWRNIVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPADKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDLAKEGQEENETCSGGNENQELQDGCSEAFEKRTSQKGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	XM_024450048.1
Organism	Homo sapiens(human)
Definition	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450048.1

ORF Insert Sequence:

ATGGTACCAT TAGGCTTCTC AGCTCCACCA GATGGCCTCC CCCAAGTCCC CCACATGGCA 
TACTGTGCTC TGGAAAACCT GTATCTTCTG ATGGGAAGGG AACTGGAATA TCTAGAGGAG 
GTACCTCCAG GAAATGTGCT AGGAATAGGA GGCCTTCAAG ATTTTGTGCT GAAATCTGCA 
ACACTGTGTA GCCTGCCATC CTGCCCACCA TTTATACCAC TCAACTTCGA AGCCACTCCT 
ATTGTGAGAG TTGCTGTTGA ACCAAAACAT CCAAGTGAAA TGCCTCAGCT CGTAAAAGGA 
ATGAAACTGT TAAACCAGGC TGATCCCTGT GTCCAGATTT TAATTCAGGA AACGGGAGAG 
CACGTTTTAG TCACAGCAGG AGAAGTCCAC CTTCAGCGAT GCCTGGATGA CTTAAAAGAA 
AGGTTTGCAA AGATTCATAT CAGTGTATCT GAACCTATTA TTCCATTCAG AGAAACAATC 
ACAAAACCCC CAAAAGTTGA CATGGTCAAT GAAGAAATAG GCAAACAGCA AAAAGTTGCA 
GTCATACACC AAATGAAAGA AGATCAAAGC AAAATCCCTG AAGGAATCCA AGTTGACTCT 
GACGGGCTAA TCACCATAAC AACTCCCAAT AAACTTGCCA CGCTCAGTGT TCGAGCCATG 
CCCCTTCCAG AAGAAGTCAC CCAGATTCTG GAAGAAAATA GTGATTTGAT TCGTTCTATG 
GAGCAGTTGA CATCCTCTTT GAATGAGGGT GAAAATACTC ACATGATTCA TCAGAAGACC 
CAAGAGAAAA TTTGGGAATT CAAAGGAAAA CTGGAGCAAC ACCTAACAGG GAGAAGATGG 
AGGAACATTG TTGACCAAAT CTGGTCATTT GGCCCAAGAA AATGTGGGCC CAACATACTA 
GTCAATAAAA GTGAAGATTT TCAGAACTCA GTATGGACAG GTCCAGCTGA CAAAGCTTCA 
AAAGAAGCCA GTAGATACCG AGATTTGGGC AATAGCATTG TGAGTGGCTT CCAACTAGCA 
ACCCTCTCTG GCCCCATGTG TGAGGAGCCT CTCATGGGTG TCTGTTTTGT TCTGGAAAAA 
TGGGACCTAA GTAAATTTGA GGAACAAGGA GCAAGTGATC TGGCAAAAGA GGGACAGGAG 
GAAAATGAAA CCTGTTCTGG TGGAAATGAA AACCAAGAGC TACAAGATGG CTGCTCTGAG 
GCCTTTGAGA AGAGGACATC ACAGAAAGGA GAATCTCCAC TCACTGACTG CTATGGACCT 
TTCTCAGGAC AGCTAATTGC CACCATGAAA GAAGCATGTC GCTATGCACT GCAAGTGAAA 
CCTCAGCGCC TGATGGCAGC TATGTACACA TGTGACATCA TGGCCACTGG TGATGTTCTC 
GGTCGAGTCT ATGCTGTCTT GTCAAAGAGA GAAGGTCGGG TACTTCAAGA AGAAATGAAA 
GAAGGGACAG ACATGTTCAT CATCAAGGCT GTGCTGCCTG TTGCTGAAAG CTTTGGTTTT 
GCTGATGAAA TCAGGAAGAG GACAAGTGGC CTGGCCAGCC CACAACTAGT ATTCAGCCAT 
TGGGAGATCA TTCCCAGTGA CCCCTTCTGG GTGCCAACTA CTGAGGAGGA ATACTTGCAC 
TTTGGGGAGA AGGCTGACTC TGAGAACCAA GCCCGGAAGT ACATGAACGC AGTACGAAAG 
CGGAAGGGGC TTTATGTGGA AGAAAAGATT GTGGAGCATG CAGAAAAGCA GAGGACACTC 
AGCAAAAATA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( NM_024580.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_078856.4 Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 1, mRNA.

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CloneID	OHu04111
Clone ID Related Accession (Same CDS sequence)	NM_024580.6 , NM_001322845.1 , NM_024580.5
Accession Version	NM_024580.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	elongation factor-like GTPase 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC026624.8, BC014628.2 and AC026956.20. On Nov 23, 2018 this sequence version replaced NM_024580.5. Transcript Variant: This variant (1) encodes the the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803615.200663.1, SRR1660809.130819.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000268206.12/ ENSP00000268206.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCTCA ACAGTTTGGA TAAGATGATT CAACTCCAGA AAAACACTGC CAACATCAGG 
AATATTTGTG TTTTGGCTCA TGTTGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTA GCAGGCAAGT TAAGGTACAT GGACAGCAGA 
GAAGATGAAC AGATCCGAGG GATCACTATG AAATCCAGTG CCATTTCCCT ACATTATGCA 
ACAGGTAATG AGGAGTACCT GATCAATCTG ATAGACTCTC CAGGACACGT GGACTTTTCC 
TCAGAAGTAT CAACCGCTGT TCGCATTTGT GATGGATGCA TCATTGTGGT AGATGCTGTG 
GAAGGAGTCT GTCCACAGAC ACAGGCAGTT CTGCGACAAG CTTGGCTTGA AAACATCCGT 
CCGGTTTTAG TGATTAATAA GATTGATCGC TTGATAGTGG AACTGAAATT CACCCCACAA 
GAGGCCTATT CTCACCTCAA GAATATTTTA GAACAGATTA ATGCGCTCAC AGGGACTCTT 
TTTACTTCTA AAGTCCTAGA AGAAAGAGCA GAGAGGGAGA CTGAATCCCA AGTGAATCCA 
AATTCTGAAC AAGGAGAGCA AGTATATGAC TGGAGCACTG GCTTGGAGGA CACAGATGAT 
TCTCACCTTT ACTTCTCTCC AGAACAGGGA AATGTGGTGT TTACCAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTCGCCAGA ATCTACAGTC AAAAAATTGG CATCAAAAAG 
GAAGTTCTTA TGAAAACCTT GTGGGGAGAT TACTATATAA ATATGAAGGC TAAAAAGATC 
ATGAAGGGTG ATCAGGCCAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTTTTGAAA AAGGACAAAG ACAAAATTGA TAAAATAGTG 
ACTTCTTTAG GATTAAAAAT TGGAGCCCGG GAGGCACGAC ATTCAGACCC TAAAGTTCAG 
ATCAACGCCA TTTGCAGTCA GTGGCTACCC ATATCCCATG CTGTTCTTGC TATGGTGTGT 
CAGAAACTTC CTAGTCCCCT TGATATTACA GCTGAGAGAG TGGAGAGACT GATGTGCACA 
GGATCACAAA CTTTTGACTC TTTTCCACCA GAAACTCAAG CACTGAAAGC AGCTTTTATG 
AAATGTGGAA GTGAGGACAC TGCTCCAGTT ATTATATTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CCTTGCCTCA GAATAAGCCA AGGCCTCTCA CTCAAGAAGA AATTGCTCAG 
AGACGTGAGC GTGCAAGACA AAGGCATGCA GAGAAGCTTG CAGCAGCACA GGGACAGGCA 
CCCTTGGAGC CCACCCAAGA TGGGAGTGCC ATTGAAACAT GTCCAAAAGG AGAGGAGCCA 
AGAGGTGACG AGCAACAGGT GGAAAGTATG ACCCCTAAAC CTGTGCTCCA GGAAGAAAAC 
AACCAAGAGT CTTTTATTGC ATTTGCTCGG GTGTTCAGTG GTGTGGCTCG AAGAGGAAAG 
AAAATTTTTG TCTTGGGGCC CAAATACAGT CCTCTTGAGT TTTTACGAAG GGTACCATTA 
GGCTTCTCAG CTCCACCAGA TGGCCTCCCC CAAGTCCCCC ACATGGCATA CTGTGCTCTG 
GAAAACCTGT ATCTTCTGAT GGGAAGGGAA CTGGAATATC TAGAGGAGGT ACCTCCAGGA 
AATGTGCTAG GAATAGGAGG CCTTCAAGAT TTTGTGCTGA AATCTGCAAC ACTGTGTAGC 
CTGCCATCCT GCCCACCATT TATACCACTC AACTTCGAAG CCACTCCTAT TGTGAGAGTT 
GCTGTTGAAC CAAAACATCC AAGTGAAATG CCTCAGCTCG TAAAAGGAAT GAAACTGTTA 
AACCAGGCTG ATCCCTGTGT CCAGATTTTA ATTCAGGAAA CGGGAGAGCA CGTTTTAGTC 
ACAGCAGGAG AAGTCCACCT TCAGCGATGC CTGGATGACT TAAAAGAAAG GTTTGCAAAG 
ATTCATATCA GTGTATCTGA ACCTATTATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATAGGC AAACAGCAAA AAGTTGCAGT CATACACCAA 
ATGAAAGAAG ATCAAAGCAA AATCCCTGAA GGAATCCAAG TTGACTCTGA CGGGCTAATC 
ACCATAACAA CTCCCAATAA ACTTGCCACG CTCAGTGTTC GAGCCATGCC CCTTCCAGAA 
GAAGTCACCC AGATTCTGGA AGAAAATAGT GATTTGATTC GTTCTATGGA GCAGTTGACA 
TCCTCTTTGA ATGAGGGTGA AAATACTCAC ATGATTCATC AGAAGACCCA AGAGAAAATT 
TGGGAATTCA AAGGAAAACT GGAGCAACAC CTAACAGGGA GAAGATGGAG GAACATTGTT 
GACCAAATCT GGTCATTTGG CCCAAGAAAA TGTGGGCCCA ACATACTAGT CAATAAAAGT 
GAAGATTTTC AGAACTCAGT ATGGACAGGT CCAGCTGACA AAGCTTCAAA AGAAGCCAGT 
AGATACCGAG ATTTGGGCAA TAGCATTGTG AGTGGCTTCC AACTAGCAAC CCTCTCTGGC 
CCCATGTGTG AGGAGCCTCT CATGGGTGTC TGTTTTGTTC TGGAAAAATG GGACCTAAGT 
AAATTTGAGG AACAAGGAGC AAGTGATCTG GCAAAAGAGG GACAGGAGGA AAATGAAACC 
TGTTCTGGTG GAAATGAAAA CCAAGAGCTA CAAGATGGCT GCTCTGAGGC CTTTGAGAAG 
AGGACATCAC AGAAAGGAGA ATCTCCACTC ACTGACTGCT ATGGACCTTT CTCAGGACAG 
CTAATTGCCA CCATGAAAGA AGCATGTCGC TATGCACTGC AAGTGAAACC TCAGCGCCTG 
ATGGCAGCTA TGTACACATG TGACATCATG GCCACTGGTG ATGTTCTCGG TCGAGTCTAT 
GCTGTCTTGT CAAAGAGAGA AGGTCGGGTA CTTCAAGAAG AAATGAAAGA AGGGACAGAC 
ATGTTCATCA TCAAGGCTGT GCTGCCTGTT GCTGAAAGCT TTGGTTTTGC TGATGAAATC 
AGGAAGAGGA CAAGTGGCCT GGCCAGCCCA CAACTAGTAT TCAGCCATTG GGAGATCATT 
CCCAGTGACC CCTTCTGGGT GCCAACTACT GAGGAGGAAT ACTTGCACTT TGGGGAGAAG 
GCTGACTCTG AGAACCAAGC CCGGAAGTAC ATGAACGCAG TACGAAAGCG GAAGGGGCTT 
TATGTGGAAG AAAAGATTGT GGAGCATGCA GAAAAGCAGA GGACACTCAG CAAAAATAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078856.4
CDS	141..3503
Translation	MVLNSLDKMIQLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQIRGITMKSSAISLHYATGNEEYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAERETESQVNPNSEQGEQVYDWSTGLEDTDDSHLYFSPEQGNVVFTSAIDGWGFGIEHFARIYSQKIGIKKEVLMKTLWGDYYINMKAKKIMKGDQAKGKKPLFVQLILENIWSLYDAVLKKDKDKIDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISHAVLAMVCQKLPSPLDITAERVERLMCTGSQTFDSFPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAQRRERARQRHAEKLAAAQGQAPLEPTQDGSAIETCPKGEEPRGDEQQVESMTPKPVLQEENNQESFIAFARVFSGVARRGKKIFVLGPKYSPLEFLRRVPLGFSAPPDGLPQVPHMAYCALENLYLLMGRELEYLEEVPPGNVLGIGGLQDFVLKSATLCSLPSCPPFIPLNFEATPIVRVAVEPKHPSEMPQLVKGMKLLNQADPCVQILIQETGEHVLVTAGEVHLQRCLDDLKERFAKIHISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQMKEDQSKIPEGIQVDSDGLITITTPNKLATLSVRAMPLPEEVTQILEENSDLIRSMEQLTSSLNEGENTHMIHQKTQEKIWEFKGKLEQHLTGRRWRNIVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPADKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDLAKEGQEENETCSGGNENQELQDGCSEAFEKRTSQKGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	NM_024580.6
Organism	Homo sapiens(human)
Definition	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024580.6

ORF Insert Sequence:

ATGGTGCTCA ACAGTTTGGA TAAGATGATT CAACTCCAGA AAAACACTGC CAACATCAGG 
AATATTTGTG TTTTGGCTCA TGTTGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTA GCAGGCAAGT TAAGGTACAT GGACAGCAGA 
GAAGATGAAC AGATCCGAGG GATCACTATG AAATCCAGTG CCATTTCCCT ACATTATGCA 
ACAGGTAATG AGGAGTACCT GATCAATCTG ATAGACTCTC CAGGACACGT GGACTTTTCC 
TCAGAAGTAT CAACCGCTGT TCGCATTTGT GATGGATGCA TCATTGTGGT AGATGCTGTG 
GAAGGAGTCT GTCCACAGAC ACAGGCAGTT CTGCGACAAG CTTGGCTTGA AAACATCCGT 
CCGGTTTTAG TGATTAATAA GATTGATCGC TTGATAGTGG AACTGAAATT CACCCCACAA 
GAGGCCTATT CTCACCTCAA GAATATTTTA GAACAGATTA ATGCGCTCAC AGGGACTCTT 
TTTACTTCTA AAGTCCTAGA AGAAAGAGCA GAGAGGGAGA CTGAATCCCA AGTGAATCCA 
AATTCTGAAC AAGGAGAGCA AGTATATGAC TGGAGCACTG GCTTGGAGGA CACAGATGAT 
TCTCACCTTT ACTTCTCTCC AGAACAGGGA AATGTGGTGT TTACCAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTCGCCAGA ATCTACAGTC AAAAAATTGG CATCAAAAAG 
GAAGTTCTTA TGAAAACCTT GTGGGGAGAT TACTATATAA ATATGAAGGC TAAAAAGATC 
ATGAAGGGTG ATCAGGCCAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTTTTGAAA AAGGACAAAG ACAAAATTGA TAAAATAGTG 
ACTTCTTTAG GATTAAAAAT TGGAGCCCGG GAGGCACGAC ATTCAGACCC TAAAGTTCAG 
ATCAACGCCA TTTGCAGTCA GTGGCTACCC ATATCCCATG CTGTTCTTGC TATGGTGTGT 
CAGAAACTTC CTAGTCCCCT TGATATTACA GCTGAGAGAG TGGAGAGACT GATGTGCACA 
GGATCACAAA CTTTTGACTC TTTTCCACCA GAAACTCAAG CACTGAAAGC AGCTTTTATG 
AAATGTGGAA GTGAGGACAC TGCTCCAGTT ATTATATTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CCTTGCCTCA GAATAAGCCA AGGCCTCTCA CTCAAGAAGA AATTGCTCAG 
AGACGTGAGC GTGCAAGACA AAGGCATGCA GAGAAGCTTG CAGCAGCACA GGGACAGGCA 
CCCTTGGAGC CCACCCAAGA TGGGAGTGCC ATTGAAACAT GTCCAAAAGG AGAGGAGCCA 
AGAGGTGACG AGCAACAGGT GGAAAGTATG ACCCCTAAAC CTGTGCTCCA GGAAGAAAAC 
AACCAAGAGT CTTTTATTGC ATTTGCTCGG GTGTTCAGTG GTGTGGCTCG AAGAGGAAAG 
AAAATTTTTG TCTTGGGGCC CAAATACAGT CCTCTTGAGT TTTTACGAAG GGTACCATTA 
GGCTTCTCAG CTCCACCAGA TGGCCTCCCC CAAGTCCCCC ACATGGCATA CTGTGCTCTG 
GAAAACCTGT ATCTTCTGAT GGGAAGGGAA CTGGAATATC TAGAGGAGGT ACCTCCAGGA 
AATGTGCTAG GAATAGGAGG CCTTCAAGAT TTTGTGCTGA AATCTGCAAC ACTGTGTAGC 
CTGCCATCCT GCCCACCATT TATACCACTC AACTTCGAAG CCACTCCTAT TGTGAGAGTT 
GCTGTTGAAC CAAAACATCC AAGTGAAATG CCTCAGCTCG TAAAAGGAAT GAAACTGTTA 
AACCAGGCTG ATCCCTGTGT CCAGATTTTA ATTCAGGAAA CGGGAGAGCA CGTTTTAGTC 
ACAGCAGGAG AAGTCCACCT TCAGCGATGC CTGGATGACT TAAAAGAAAG GTTTGCAAAG 
ATTCATATCA GTGTATCTGA ACCTATTATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATAGGC AAACAGCAAA AAGTTGCAGT CATACACCAA 
ATGAAAGAAG ATCAAAGCAA AATCCCTGAA GGAATCCAAG TTGACTCTGA CGGGCTAATC 
ACCATAACAA CTCCCAATAA ACTTGCCACG CTCAGTGTTC GAGCCATGCC CCTTCCAGAA 
GAAGTCACCC AGATTCTGGA AGAAAATAGT GATTTGATTC GTTCTATGGA GCAGTTGACA 
TCCTCTTTGA ATGAGGGTGA AAATACTCAC ATGATTCATC AGAAGACCCA AGAGAAAATT 
TGGGAATTCA AAGGAAAACT GGAGCAACAC CTAACAGGGA GAAGATGGAG GAACATTGTT 
GACCAAATCT GGTCATTTGG CCCAAGAAAA TGTGGGCCCA ACATACTAGT CAATAAAAGT 
GAAGATTTTC AGAACTCAGT ATGGACAGGT CCAGCTGACA AAGCTTCAAA AGAAGCCAGT 
AGATACCGAG ATTTGGGCAA TAGCATTGTG AGTGGCTTCC AACTAGCAAC CCTCTCTGGC 
CCCATGTGTG AGGAGCCTCT CATGGGTGTC TGTTTTGTTC TGGAAAAATG GGACCTAAGT 
AAATTTGAGG AACAAGGAGC AAGTGATCTG GCAAAAGAGG GACAGGAGGA AAATGAAACC 
TGTTCTGGTG GAAATGAAAA CCAAGAGCTA CAAGATGGCT GCTCTGAGGC CTTTGAGAAG 
AGGACATCAC AGAAAGGAGA ATCTCCACTC ACTGACTGCT ATGGACCTTT CTCAGGACAG 
CTAATTGCCA CCATGAAAGA AGCATGTCGC TATGCACTGC AAGTGAAACC TCAGCGCCTG 
ATGGCAGCTA TGTACACATG TGACATCATG GCCACTGGTG ATGTTCTCGG TCGAGTCTAT 
GCTGTCTTGT CAAAGAGAGA AGGTCGGGTA CTTCAAGAAG AAATGAAAGA AGGGACAGAC 
ATGTTCATCA TCAAGGCTGT GCTGCCTGTT GCTGAAAGCT TTGGTTTTGC TGATGAAATC 
AGGAAGAGGA CAAGTGGCCT GGCCAGCCCA CAACTAGTAT TCAGCCATTG GGAGATCATT 
CCCAGTGACC CCTTCTGGGT GCCAACTACT GAGGAGGAAT ACTTGCACTT TGGGGAGAAG 
GCTGACTCTG AGAACCAAGC CCGGAAGTAC ATGAACGCAG TACGAAAGCG GAAGGGGCTT 
TATGTGGAAG AAAAGATTGT GGAGCATGCA GAAAAGCAGA GGACACTCAG CAAAAATAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( NM_001322845.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309774.1 Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu04111
Clone ID Related Accession (Same CDS sequence)	NM_024580.6 , NM_001322845.1 , NM_024580.5
Accession Version	NM_001322845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	elongation factor-like GTPase 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC026624.8 and AC026956.20. On Apr 13, 2016 this sequence version replaced XM_011521998.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.216194.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGTGCTCA ACAGTTTGGA TAAGATGATT CAACTCCAGA AAAACACTGC CAACATCAGG 
AATATTTGTG TTTTGGCTCA TGTTGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTA GCAGGCAAGT TAAGGTACAT GGACAGCAGA 
GAAGATGAAC AGATCCGAGG GATCACTATG AAATCCAGTG CCATTTCCCT ACATTATGCA 
ACAGGTAATG AGGAGTACCT GATCAATCTG ATAGACTCTC CAGGACACGT GGACTTTTCC 
TCAGAAGTAT CAACCGCTGT TCGCATTTGT GATGGATGCA TCATTGTGGT AGATGCTGTG 
GAAGGAGTCT GTCCACAGAC ACAGGCAGTT CTGCGACAAG CTTGGCTTGA AAACATCCGT 
CCGGTTTTAG TGATTAATAA GATTGATCGC TTGATAGTGG AACTGAAATT CACCCCACAA 
GAGGCCTATT CTCACCTCAA GAATATTTTA GAACAGATTA ATGCGCTCAC AGGGACTCTT 
TTTACTTCTA AAGTCCTAGA AGAAAGAGCA GAGAGGGAGA CTGAATCCCA AGTGAATCCA 
AATTCTGAAC AAGGAGAGCA AGTATATGAC TGGAGCACTG GCTTGGAGGA CACAGATGAT 
TCTCACCTTT ACTTCTCTCC AGAACAGGGA AATGTGGTGT TTACCAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTCGCCAGA ATCTACAGTC AAAAAATTGG CATCAAAAAG 
GAAGTTCTTA TGAAAACCTT GTGGGGAGAT TACTATATAA ATATGAAGGC TAAAAAGATC 
ATGAAGGGTG ATCAGGCCAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTTTTGAAA AAGGACAAAG ACAAAATTGA TAAAATAGTG 
ACTTCTTTAG GATTAAAAAT TGGAGCCCGG GAGGCACGAC ATTCAGACCC TAAAGTTCAG 
ATCAACGCCA TTTGCAGTCA GTGGCTACCC ATATCCCATG CTGTTCTTGC TATGGTGTGT 
CAGAAACTTC CTAGTCCCCT TGATATTACA GCTGAGAGAG TGGAGAGACT GATGTGCACA 
GGATCACAAA CTTTTGACTC TTTTCCACCA GAAACTCAAG CACTGAAAGC AGCTTTTATG 
AAATGTGGAA GTGAGGACAC TGCTCCAGTT ATTATATTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CCTTGCCTCA GAATAAGCCA AGGCCTCTCA CTCAAGAAGA AATTGCTCAG 
AGACGTGAGC GTGCAAGACA AAGGCATGCA GAGAAGCTTG CAGCAGCACA GGGACAGGCA 
CCCTTGGAGC CCACCCAAGA TGGGAGTGCC ATTGAAACAT GTCCAAAAGG AGAGGAGCCA 
AGAGGTGACG AGCAACAGGT GGAAAGTATG ACCCCTAAAC CTGTGCTCCA GGAAGAAAAC 
AACCAAGAGT CTTTTATTGC ATTTGCTCGG GTGTTCAGTG GTGTGGCTCG AAGAGGAAAG 
AAAATTTTTG TCTTGGGGCC CAAATACAGT CCTCTTGAGT TTTTACGAAG GGTACCATTA 
GGCTTCTCAG CTCCACCAGA TGGCCTCCCC CAAGTCCCCC ACATGGCATA CTGTGCTCTG 
GAAAACCTGT ATCTTCTGAT GGGAAGGGAA CTGGAATATC TAGAGGAGGT ACCTCCAGGA 
AATGTGCTAG GAATAGGAGG CCTTCAAGAT TTTGTGCTGA AATCTGCAAC ACTGTGTAGC 
CTGCCATCCT GCCCACCATT TATACCACTC AACTTCGAAG CCACTCCTAT TGTGAGAGTT 
GCTGTTGAAC CAAAACATCC AAGTGAAATG CCTCAGCTCG TAAAAGGAAT GAAACTGTTA 
AACCAGGCTG ATCCCTGTGT CCAGATTTTA ATTCAGGAAA CGGGAGAGCA CGTTTTAGTC 
ACAGCAGGAG AAGTCCACCT TCAGCGATGC CTGGATGACT TAAAAGAAAG GTTTGCAAAG 
ATTCATATCA GTGTATCTGA ACCTATTATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATAGGC AAACAGCAAA AAGTTGCAGT CATACACCAA 
ATGAAAGAAG ATCAAAGCAA AATCCCTGAA GGAATCCAAG TTGACTCTGA CGGGCTAATC 
ACCATAACAA CTCCCAATAA ACTTGCCACG CTCAGTGTTC GAGCCATGCC CCTTCCAGAA 
GAAGTCACCC AGATTCTGGA AGAAAATAGT GATTTGATTC GTTCTATGGA GCAGTTGACA 
TCCTCTTTGA ATGAGGGTGA AAATACTCAC ATGATTCATC AGAAGACCCA AGAGAAAATT 
TGGGAATTCA AAGGAAAACT GGAGCAACAC CTAACAGGGA GAAGATGGAG GAACATTGTT 
GACCAAATCT GGTCATTTGG CCCAAGAAAA TGTGGGCCCA ACATACTAGT CAATAAAAGT 
GAAGATTTTC AGAACTCAGT ATGGACAGGT CCAGCTGACA AAGCTTCAAA AGAAGCCAGT 
AGATACCGAG ATTTGGGCAA TAGCATTGTG AGTGGCTTCC AACTAGCAAC CCTCTCTGGC 
CCCATGTGTG AGGAGCCTCT CATGGGTGTC TGTTTTGTTC TGGAAAAATG GGACCTAAGT 
AAATTTGAGG AACAAGGAGC AAGTGATCTG GCAAAAGAGG GACAGGAGGA AAATGAAACC 
TGTTCTGGTG GAAATGAAAA CCAAGAGCTA CAAGATGGCT GCTCTGAGGC CTTTGAGAAG 
AGGACATCAC AGAAAGGAGA ATCTCCACTC ACTGACTGCT ATGGACCTTT CTCAGGACAG 
CTAATTGCCA CCATGAAAGA AGCATGTCGC TATGCACTGC AAGTGAAACC TCAGCGCCTG 
ATGGCAGCTA TGTACACATG TGACATCATG GCCACTGGTG ATGTTCTCGG TCGAGTCTAT 
GCTGTCTTGT CAAAGAGAGA AGGTCGGGTA CTTCAAGAAG AAATGAAAGA AGGGACAGAC 
ATGTTCATCA TCAAGGCTGT GCTGCCTGTT GCTGAAAGCT TTGGTTTTGC TGATGAAATC 
AGGAAGAGGA CAAGTGGCCT GGCCAGCCCA CAACTAGTAT TCAGCCATTG GGAGATCATT 
CCCAGTGACC CCTTCTGGGT GCCAACTACT GAGGAGGAAT ACTTGCACTT TGGGGAGAAG 
GCTGACTCTG AGAACCAAGC CCGGAAGTAC ATGAACGCAG TACGAAAGCG GAAGGGGCTT 
TATGTGGAAG AAAAGATTGT GGAGCATGCA GAAAAGCAGA GGACACTCAG CAAAAATAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309774.1
CDS	412..3774
Translation	MVLNSLDKMIQLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQIRGITMKSSAISLHYATGNEEYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAERETESQVNPNSEQGEQVYDWSTGLEDTDDSHLYFSPEQGNVVFTSAIDGWGFGIEHFARIYSQKIGIKKEVLMKTLWGDYYINMKAKKIMKGDQAKGKKPLFVQLILENIWSLYDAVLKKDKDKIDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISHAVLAMVCQKLPSPLDITAERVERLMCTGSQTFDSFPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAQRRERARQRHAEKLAAAQGQAPLEPTQDGSAIETCPKGEEPRGDEQQVESMTPKPVLQEENNQESFIAFARVFSGVARRGKKIFVLGPKYSPLEFLRRVPLGFSAPPDGLPQVPHMAYCALENLYLLMGRELEYLEEVPPGNVLGIGGLQDFVLKSATLCSLPSCPPFIPLNFEATPIVRVAVEPKHPSEMPQLVKGMKLLNQADPCVQILIQETGEHVLVTAGEVHLQRCLDDLKERFAKIHISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQMKEDQSKIPEGIQVDSDGLITITTPNKLATLSVRAMPLPEEVTQILEENSDLIRSMEQLTSSLNEGENTHMIHQKTQEKIWEFKGKLEQHLTGRRWRNIVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPADKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDLAKEGQEENETCSGGNENQELQDGCSEAFEKRTSQKGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	NM_001322845.1
Organism	Homo sapiens(human)
Definition	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322845.1

ORF Insert Sequence:

ATGGTGCTCA ACAGTTTGGA TAAGATGATT CAACTCCAGA AAAACACTGC CAACATCAGG 
AATATTTGTG TTTTGGCTCA TGTTGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTA GCAGGCAAGT TAAGGTACAT GGACAGCAGA 
GAAGATGAAC AGATCCGAGG GATCACTATG AAATCCAGTG CCATTTCCCT ACATTATGCA 
ACAGGTAATG AGGAGTACCT GATCAATCTG ATAGACTCTC CAGGACACGT GGACTTTTCC 
TCAGAAGTAT CAACCGCTGT TCGCATTTGT GATGGATGCA TCATTGTGGT AGATGCTGTG 
GAAGGAGTCT GTCCACAGAC ACAGGCAGTT CTGCGACAAG CTTGGCTTGA AAACATCCGT 
CCGGTTTTAG TGATTAATAA GATTGATCGC TTGATAGTGG AACTGAAATT CACCCCACAA 
GAGGCCTATT CTCACCTCAA GAATATTTTA GAACAGATTA ATGCGCTCAC AGGGACTCTT 
TTTACTTCTA AAGTCCTAGA AGAAAGAGCA GAGAGGGAGA CTGAATCCCA AGTGAATCCA 
AATTCTGAAC AAGGAGAGCA AGTATATGAC TGGAGCACTG GCTTGGAGGA CACAGATGAT 
TCTCACCTTT ACTTCTCTCC AGAACAGGGA AATGTGGTGT TTACCAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTCGCCAGA ATCTACAGTC AAAAAATTGG CATCAAAAAG 
GAAGTTCTTA TGAAAACCTT GTGGGGAGAT TACTATATAA ATATGAAGGC TAAAAAGATC 
ATGAAGGGTG ATCAGGCCAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTTTTGAAA AAGGACAAAG ACAAAATTGA TAAAATAGTG 
ACTTCTTTAG GATTAAAAAT TGGAGCCCGG GAGGCACGAC ATTCAGACCC TAAAGTTCAG 
ATCAACGCCA TTTGCAGTCA GTGGCTACCC ATATCCCATG CTGTTCTTGC TATGGTGTGT 
CAGAAACTTC CTAGTCCCCT TGATATTACA GCTGAGAGAG TGGAGAGACT GATGTGCACA 
GGATCACAAA CTTTTGACTC TTTTCCACCA GAAACTCAAG CACTGAAAGC AGCTTTTATG 
AAATGTGGAA GTGAGGACAC TGCTCCAGTT ATTATATTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CCTTGCCTCA GAATAAGCCA AGGCCTCTCA CTCAAGAAGA AATTGCTCAG 
AGACGTGAGC GTGCAAGACA AAGGCATGCA GAGAAGCTTG CAGCAGCACA GGGACAGGCA 
CCCTTGGAGC CCACCCAAGA TGGGAGTGCC ATTGAAACAT GTCCAAAAGG AGAGGAGCCA 
AGAGGTGACG AGCAACAGGT GGAAAGTATG ACCCCTAAAC CTGTGCTCCA GGAAGAAAAC 
AACCAAGAGT CTTTTATTGC ATTTGCTCGG GTGTTCAGTG GTGTGGCTCG AAGAGGAAAG 
AAAATTTTTG TCTTGGGGCC CAAATACAGT CCTCTTGAGT TTTTACGAAG GGTACCATTA 
GGCTTCTCAG CTCCACCAGA TGGCCTCCCC CAAGTCCCCC ACATGGCATA CTGTGCTCTG 
GAAAACCTGT ATCTTCTGAT GGGAAGGGAA CTGGAATATC TAGAGGAGGT ACCTCCAGGA 
AATGTGCTAG GAATAGGAGG CCTTCAAGAT TTTGTGCTGA AATCTGCAAC ACTGTGTAGC 
CTGCCATCCT GCCCACCATT TATACCACTC AACTTCGAAG CCACTCCTAT TGTGAGAGTT 
GCTGTTGAAC CAAAACATCC AAGTGAAATG CCTCAGCTCG TAAAAGGAAT GAAACTGTTA 
AACCAGGCTG ATCCCTGTGT CCAGATTTTA ATTCAGGAAA CGGGAGAGCA CGTTTTAGTC 
ACAGCAGGAG AAGTCCACCT TCAGCGATGC CTGGATGACT TAAAAGAAAG GTTTGCAAAG 
ATTCATATCA GTGTATCTGA ACCTATTATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATAGGC AAACAGCAAA AAGTTGCAGT CATACACCAA 
ATGAAAGAAG ATCAAAGCAA AATCCCTGAA GGAATCCAAG TTGACTCTGA CGGGCTAATC 
ACCATAACAA CTCCCAATAA ACTTGCCACG CTCAGTGTTC GAGCCATGCC CCTTCCAGAA 
GAAGTCACCC AGATTCTGGA AGAAAATAGT GATTTGATTC GTTCTATGGA GCAGTTGACA 
TCCTCTTTGA ATGAGGGTGA AAATACTCAC ATGATTCATC AGAAGACCCA AGAGAAAATT 
TGGGAATTCA AAGGAAAACT GGAGCAACAC CTAACAGGGA GAAGATGGAG GAACATTGTT 
GACCAAATCT GGTCATTTGG CCCAAGAAAA TGTGGGCCCA ACATACTAGT CAATAAAAGT 
GAAGATTTTC AGAACTCAGT ATGGACAGGT CCAGCTGACA AAGCTTCAAA AGAAGCCAGT 
AGATACCGAG ATTTGGGCAA TAGCATTGTG AGTGGCTTCC AACTAGCAAC CCTCTCTGGC 
CCCATGTGTG AGGAGCCTCT CATGGGTGTC TGTTTTGTTC TGGAAAAATG GGACCTAAGT 
AAATTTGAGG AACAAGGAGC AAGTGATCTG GCAAAAGAGG GACAGGAGGA AAATGAAACC 
TGTTCTGGTG GAAATGAAAA CCAAGAGCTA CAAGATGGCT GCTCTGAGGC CTTTGAGAAG 
AGGACATCAC AGAAAGGAGA ATCTCCACTC ACTGACTGCT ATGGACCTTT CTCAGGACAG 
CTAATTGCCA CCATGAAAGA AGCATGTCGC TATGCACTGC AAGTGAAACC TCAGCGCCTG 
ATGGCAGCTA TGTACACATG TGACATCATG GCCACTGGTG ATGTTCTCGG TCGAGTCTAT 
GCTGTCTTGT CAAAGAGAGA AGGTCGGGTA CTTCAAGAAG AAATGAAAGA AGGGACAGAC 
ATGTTCATCA TCAAGGCTGT GCTGCCTGTT GCTGAAAGCT TTGGTTTTGC TGATGAAATC 
AGGAAGAGGA CAAGTGGCCT GGCCAGCCCA CAACTAGTAT TCAGCCATTG GGAGATCATT 
CCCAGTGACC CCTTCTGGGT GCCAACTACT GAGGAGGAAT ACTTGCACTT TGGGGAGAAG 
GCTGACTCTG AGAACCAAGC CCGGAAGTAC ATGAACGCAG TACGAAAGCG GAAGGGGCTT 
TATGTGGAAG AAAAGATTGT GGAGCATGCA GAAAAGCAGA GGACACTCAG CAAAAATAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( NM_024580.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_078856.4 Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04111
Clone ID Related Accession (Same CDS sequence)	NM_024580.6 , NM_001322845.1 , NM_024580.5
Accession Version	NM_024580.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	elongation factor-like GTPase 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC026624.8, BC014628.2 and AC026956.20. On Aug 3, 2006 this sequence version replaced NM_024580.4. Transcript Variant: This variant (1) encodes the the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803615.200663.1, SRR1803613.8908.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCTCA ACAGTTTGGA TAAGATGATT CAACTCCAGA AAAACACTGC CAACATCAGG 
AATATTTGTG TTTTGGCTCA TGTTGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTA GCAGGCAAGT TAAGGTACAT GGACAGCAGA 
GAAGATGAAC AGATCCGAGG GATCACTATG AAATCCAGTG CCATTTCCCT ACATTATGCA 
ACAGGTAATG AGGAGTACCT GATCAATCTG ATAGACTCTC CAGGACACGT GGACTTTTCC 
TCAGAAGTAT CAACCGCTGT TCGCATTTGT GATGGATGCA TCATTGTGGT AGATGCTGTG 
GAAGGAGTCT GTCCACAGAC ACAGGCAGTT CTGCGACAAG CTTGGCTTGA AAACATCCGT 
CCGGTTTTAG TGATTAATAA GATTGATCGC TTGATAGTGG AACTGAAATT CACCCCACAA 
GAGGCCTATT CTCACCTCAA GAATATTTTA GAACAGATTA ATGCGCTCAC AGGGACTCTT 
TTTACTTCTA AAGTCCTAGA AGAAAGAGCA GAGAGGGAGA CTGAATCCCA AGTGAATCCA 
AATTCTGAAC AAGGAGAGCA AGTATATGAC TGGAGCACTG GCTTGGAGGA CACAGATGAT 
TCTCACCTTT ACTTCTCTCC AGAACAGGGA AATGTGGTGT TTACCAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTCGCCAGA ATCTACAGTC AAAAAATTGG CATCAAAAAG 
GAAGTTCTTA TGAAAACCTT GTGGGGAGAT TACTATATAA ATATGAAGGC TAAAAAGATC 
ATGAAGGGTG ATCAGGCCAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTTTTGAAA AAGGACAAAG ACAAAATTGA TAAAATAGTG 
ACTTCTTTAG GATTAAAAAT TGGAGCCCGG GAGGCACGAC ATTCAGACCC TAAAGTTCAG 
ATCAACGCCA TTTGCAGTCA GTGGCTACCC ATATCCCATG CTGTTCTTGC TATGGTGTGT 
CAGAAACTTC CTAGTCCCCT TGATATTACA GCTGAGAGAG TGGAGAGACT GATGTGCACA 
GGATCACAAA CTTTTGACTC TTTTCCACCA GAAACTCAAG CACTGAAAGC AGCTTTTATG 
AAATGTGGAA GTGAGGACAC TGCTCCAGTT ATTATATTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CCTTGCCTCA GAATAAGCCA AGGCCTCTCA CTCAAGAAGA AATTGCTCAG 
AGACGTGAGC GTGCAAGACA AAGGCATGCA GAGAAGCTTG CAGCAGCACA GGGACAGGCA 
CCCTTGGAGC CCACCCAAGA TGGGAGTGCC ATTGAAACAT GTCCAAAAGG AGAGGAGCCA 
AGAGGTGACG AGCAACAGGT GGAAAGTATG ACCCCTAAAC CTGTGCTCCA GGAAGAAAAC 
AACCAAGAGT CTTTTATTGC ATTTGCTCGG GTGTTCAGTG GTGTGGCTCG AAGAGGAAAG 
AAAATTTTTG TCTTGGGGCC CAAATACAGT CCTCTTGAGT TTTTACGAAG GGTACCATTA 
GGCTTCTCAG CTCCACCAGA TGGCCTCCCC CAAGTCCCCC ACATGGCATA CTGTGCTCTG 
GAAAACCTGT ATCTTCTGAT GGGAAGGGAA CTGGAATATC TAGAGGAGGT ACCTCCAGGA 
AATGTGCTAG GAATAGGAGG CCTTCAAGAT TTTGTGCTGA AATCTGCAAC ACTGTGTAGC 
CTGCCATCCT GCCCACCATT TATACCACTC AACTTCGAAG CCACTCCTAT TGTGAGAGTT 
GCTGTTGAAC CAAAACATCC AAGTGAAATG CCTCAGCTCG TAAAAGGAAT GAAACTGTTA 
AACCAGGCTG ATCCCTGTGT CCAGATTTTA ATTCAGGAAA CGGGAGAGCA CGTTTTAGTC 
ACAGCAGGAG AAGTCCACCT TCAGCGATGC CTGGATGACT TAAAAGAAAG GTTTGCAAAG 
ATTCATATCA GTGTATCTGA ACCTATTATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATAGGC AAACAGCAAA AAGTTGCAGT CATACACCAA 
ATGAAAGAAG ATCAAAGCAA AATCCCTGAA GGAATCCAAG TTGACTCTGA CGGGCTAATC 
ACCATAACAA CTCCCAATAA ACTTGCCACG CTCAGTGTTC GAGCCATGCC CCTTCCAGAA 
GAAGTCACCC AGATTCTGGA AGAAAATAGT GATTTGATTC GTTCTATGGA GCAGTTGACA 
TCCTCTTTGA ATGAGGGTGA AAATACTCAC ATGATTCATC AGAAGACCCA AGAGAAAATT 
TGGGAATTCA AAGGAAAACT GGAGCAACAC CTAACAGGGA GAAGATGGAG GAACATTGTT 
GACCAAATCT GGTCATTTGG CCCAAGAAAA TGTGGGCCCA ACATACTAGT CAATAAAAGT 
GAAGATTTTC AGAACTCAGT ATGGACAGGT CCAGCTGACA AAGCTTCAAA AGAAGCCAGT 
AGATACCGAG ATTTGGGCAA TAGCATTGTG AGTGGCTTCC AACTAGCAAC CCTCTCTGGC 
CCCATGTGTG AGGAGCCTCT CATGGGTGTC TGTTTTGTTC TGGAAAAATG GGACCTAAGT 
AAATTTGAGG AACAAGGAGC AAGTGATCTG GCAAAAGAGG GACAGGAGGA AAATGAAACC 
TGTTCTGGTG GAAATGAAAA CCAAGAGCTA CAAGATGGCT GCTCTGAGGC CTTTGAGAAG 
AGGACATCAC AGAAAGGAGA ATCTCCACTC ACTGACTGCT ATGGACCTTT CTCAGGACAG 
CTAATTGCCA CCATGAAAGA AGCATGTCGC TATGCACTGC AAGTGAAACC TCAGCGCCTG 
ATGGCAGCTA TGTACACATG TGACATCATG GCCACTGGTG ATGTTCTCGG TCGAGTCTAT 
GCTGTCTTGT CAAAGAGAGA AGGTCGGGTA CTTCAAGAAG AAATGAAAGA AGGGACAGAC 
ATGTTCATCA TCAAGGCTGT GCTGCCTGTT GCTGAAAGCT TTGGTTTTGC TGATGAAATC 
AGGAAGAGGA CAAGTGGCCT GGCCAGCCCA CAACTAGTAT TCAGCCATTG GGAGATCATT 
CCCAGTGACC CCTTCTGGGT GCCAACTACT GAGGAGGAAT ACTTGCACTT TGGGGAGAAG 
GCTGACTCTG AGAACCAAGC CCGGAAGTAC ATGAACGCAG TACGAAAGCG GAAGGGGCTT 
TATGTGGAAG AAAAGATTGT GGAGCATGCA GAAAAGCAGA GGACACTCAG CAAAAATAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078856.4
CDS	170..3532
Translation	MVLNSLDKMIQLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQIRGITMKSSAISLHYATGNEEYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAERETESQVNPNSEQGEQVYDWSTGLEDTDDSHLYFSPEQGNVVFTSAIDGWGFGIEHFARIYSQKIGIKKEVLMKTLWGDYYINMKAKKIMKGDQAKGKKPLFVQLILENIWSLYDAVLKKDKDKIDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISHAVLAMVCQKLPSPLDITAERVERLMCTGSQTFDSFPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAQRRERARQRHAEKLAAAQGQAPLEPTQDGSAIETCPKGEEPRGDEQQVESMTPKPVLQEENNQESFIAFARVFSGVARRGKKIFVLGPKYSPLEFLRRVPLGFSAPPDGLPQVPHMAYCALENLYLLMGRELEYLEEVPPGNVLGIGGLQDFVLKSATLCSLPSCPPFIPLNFEATPIVRVAVEPKHPSEMPQLVKGMKLLNQADPCVQILIQETGEHVLVTAGEVHLQRCLDDLKERFAKIHISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQMKEDQSKIPEGIQVDSDGLITITTPNKLATLSVRAMPLPEEVTQILEENSDLIRSMEQLTSSLNEGENTHMIHQKTQEKIWEFKGKLEQHLTGRRWRNIVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPADKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDLAKEGQEENETCSGGNENQELQDGCSEAFEKRTSQKGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	NM_024580.5
Organism	Homo sapiens(human)
Definition	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024580.5

ORF Insert Sequence:

ATGGTGCTCA ACAGTTTGGA TAAGATGATT CAACTCCAGA AAAACACTGC CAACATCAGG 
AATATTTGTG TTTTGGCTCA TGTTGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTA GCAGGCAAGT TAAGGTACAT GGACAGCAGA 
GAAGATGAAC AGATCCGAGG GATCACTATG AAATCCAGTG CCATTTCCCT ACATTATGCA 
ACAGGTAATG AGGAGTACCT GATCAATCTG ATAGACTCTC CAGGACACGT GGACTTTTCC 
TCAGAAGTAT CAACCGCTGT TCGCATTTGT GATGGATGCA TCATTGTGGT AGATGCTGTG 
GAAGGAGTCT GTCCACAGAC ACAGGCAGTT CTGCGACAAG CTTGGCTTGA AAACATCCGT 
CCGGTTTTAG TGATTAATAA GATTGATCGC TTGATAGTGG AACTGAAATT CACCCCACAA 
GAGGCCTATT CTCACCTCAA GAATATTTTA GAACAGATTA ATGCGCTCAC AGGGACTCTT 
TTTACTTCTA AAGTCCTAGA AGAAAGAGCA GAGAGGGAGA CTGAATCCCA AGTGAATCCA 
AATTCTGAAC AAGGAGAGCA AGTATATGAC TGGAGCACTG GCTTGGAGGA CACAGATGAT 
TCTCACCTTT ACTTCTCTCC AGAACAGGGA AATGTGGTGT TTACCAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTCGCCAGA ATCTACAGTC AAAAAATTGG CATCAAAAAG 
GAAGTTCTTA TGAAAACCTT GTGGGGAGAT TACTATATAA ATATGAAGGC TAAAAAGATC 
ATGAAGGGTG ATCAGGCCAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTTTTGAAA AAGGACAAAG ACAAAATTGA TAAAATAGTG 
ACTTCTTTAG GATTAAAAAT TGGAGCCCGG GAGGCACGAC ATTCAGACCC TAAAGTTCAG 
ATCAACGCCA TTTGCAGTCA GTGGCTACCC ATATCCCATG CTGTTCTTGC TATGGTGTGT 
CAGAAACTTC CTAGTCCCCT TGATATTACA GCTGAGAGAG TGGAGAGACT GATGTGCACA 
GGATCACAAA CTTTTGACTC TTTTCCACCA GAAACTCAAG CACTGAAAGC AGCTTTTATG 
AAATGTGGAA GTGAGGACAC TGCTCCAGTT ATTATATTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CCTTGCCTCA GAATAAGCCA AGGCCTCTCA CTCAAGAAGA AATTGCTCAG 
AGACGTGAGC GTGCAAGACA AAGGCATGCA GAGAAGCTTG CAGCAGCACA GGGACAGGCA 
CCCTTGGAGC CCACCCAAGA TGGGAGTGCC ATTGAAACAT GTCCAAAAGG AGAGGAGCCA 
AGAGGTGACG AGCAACAGGT GGAAAGTATG ACCCCTAAAC CTGTGCTCCA GGAAGAAAAC 
AACCAAGAGT CTTTTATTGC ATTTGCTCGG GTGTTCAGTG GTGTGGCTCG AAGAGGAAAG 
AAAATTTTTG TCTTGGGGCC CAAATACAGT CCTCTTGAGT TTTTACGAAG GGTACCATTA 
GGCTTCTCAG CTCCACCAGA TGGCCTCCCC CAAGTCCCCC ACATGGCATA CTGTGCTCTG 
GAAAACCTGT ATCTTCTGAT GGGAAGGGAA CTGGAATATC TAGAGGAGGT ACCTCCAGGA 
AATGTGCTAG GAATAGGAGG CCTTCAAGAT TTTGTGCTGA AATCTGCAAC ACTGTGTAGC 
CTGCCATCCT GCCCACCATT TATACCACTC AACTTCGAAG CCACTCCTAT TGTGAGAGTT 
GCTGTTGAAC CAAAACATCC AAGTGAAATG CCTCAGCTCG TAAAAGGAAT GAAACTGTTA 
AACCAGGCTG ATCCCTGTGT CCAGATTTTA ATTCAGGAAA CGGGAGAGCA CGTTTTAGTC 
ACAGCAGGAG AAGTCCACCT TCAGCGATGC CTGGATGACT TAAAAGAAAG GTTTGCAAAG 
ATTCATATCA GTGTATCTGA ACCTATTATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATAGGC AAACAGCAAA AAGTTGCAGT CATACACCAA 
ATGAAAGAAG ATCAAAGCAA AATCCCTGAA GGAATCCAAG TTGACTCTGA CGGGCTAATC 
ACCATAACAA CTCCCAATAA ACTTGCCACG CTCAGTGTTC GAGCCATGCC CCTTCCAGAA 
GAAGTCACCC AGATTCTGGA AGAAAATAGT GATTTGATTC GTTCTATGGA GCAGTTGACA 
TCCTCTTTGA ATGAGGGTGA AAATACTCAC ATGATTCATC AGAAGACCCA AGAGAAAATT 
TGGGAATTCA AAGGAAAACT GGAGCAACAC CTAACAGGGA GAAGATGGAG GAACATTGTT 
GACCAAATCT GGTCATTTGG CCCAAGAAAA TGTGGGCCCA ACATACTAGT CAATAAAAGT 
GAAGATTTTC AGAACTCAGT ATGGACAGGT CCAGCTGACA AAGCTTCAAA AGAAGCCAGT 
AGATACCGAG ATTTGGGCAA TAGCATTGTG AGTGGCTTCC AACTAGCAAC CCTCTCTGGC 
CCCATGTGTG AGGAGCCTCT CATGGGTGTC TGTTTTGTTC TGGAAAAATG GGACCTAAGT 
AAATTTGAGG AACAAGGAGC AAGTGATCTG GCAAAAGAGG GACAGGAGGA AAATGAAACC 
TGTTCTGGTG GAAATGAAAA CCAAGAGCTA CAAGATGGCT GCTCTGAGGC CTTTGAGAAG 
AGGACATCAC AGAAAGGAGA ATCTCCACTC ACTGACTGCT ATGGACCTTT CTCAGGACAG 
CTAATTGCCA CCATGAAAGA AGCATGTCGC TATGCACTGC AAGTGAAACC TCAGCGCCTG 
ATGGCAGCTA TGTACACATG TGACATCATG GCCACTGGTG ATGTTCTCGG TCGAGTCTAT 
GCTGTCTTGT CAAAGAGAGA AGGTCGGGTA CTTCAAGAAG AAATGAAAGA AGGGACAGAC 
ATGTTCATCA TCAAGGCTGT GCTGCCTGTT GCTGAAAGCT TTGGTTTTGC TGATGAAATC 
AGGAAGAGGA CAAGTGGCCT GGCCAGCCCA CAACTAGTAT TCAGCCATTG GGAGATCATT 
CCCAGTGACC CCTTCTGGGT GCCAACTACT GAGGAGGAAT ACTTGCACTT TGGGGAGAAG 
GCTGACTCTG AGAACCAAGC CCGGAAGTAC ATGAACGCAG TACGAAAGCG GAAGGGGCTT 
TATGTGGAAG AAAAGATTGT GGAGCATGCA GAAAAGCAGA GGACACTCAG CAAAAATAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( NM_001040610.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035700.1 Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 2, mRNA.

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CloneID	OHu04996
Clone ID Related Accession (Same CDS sequence)	NM_001040610.2
Accession Version	NM_001040610.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	elongation factor-like GTPase 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC026624.8, BC014628.2 and AC026956.20. On Aug 3, 2006 this sequence version replaced NM_001040610.1. Transcript Variant: This variant (2) lacks two in-frame exons in the 5' coding region, compared to variant 1, which results in a shorter protein (isoform 2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.69522.1, BX538332.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCTCA ACAGTTTGGA TAAGATGATT CAACTCCAGA AAAACACTGC CAACATCAGG 
AATATTTGTG TTTTGGCTCA TGTTGACCAT GGTAATGAGG AGTACCTGAT CAATCTGATA 
GACTCTCCAG GACACGTGGA CTTTTCCTCA GAAGTATCAA CCGCTGTTCG CATTTGTGAT 
GGATGCATCA TTGTGGTAGA TGCTGTGGAA GGAGTCTGTC CACAGACACA GGCAGTTCTG 
CGACAAGCTT GGCTTGAAAA CATCCGTCCG GTTTTAGTGA TTAATAAGAT TGATCGCTTG 
ATAGTGGAAC TGAAATTCAC CCCACAAGAG GCCTATTCTC ACCTCAAGAA TATTTTAGAA 
CAGATTAATG CGCTCACAGG GACTCTTTTT ACTTCTAAAG TCCTAGAAGA AAGAGCAGAG 
AGGGAGACTG AATCCCAAGT GAATCCAAAT TCTGAACAAG GAGAGCAAGT ATATGACTGG 
AGCACTGGCT TGGAGGACAC AGATGATTCT CACCTTTACT TCTCTCCAGA ACAGGGAAAT 
GTGGTGTTTA CCAGTGCAAT AGATGGGTGG GGCTTTGGAA TTGAGCACTT CGCCAGAATC 
TACAGTCAAA AAATTGGCAT CAAAAAGGAA GTTCTTATGA AAACCTTGTG GGGAGATTAC 
TATATAAATA TGAAGGCTAA AAAGATCATG AAGGGTGATC AGGCCAAAGG AAAGAAACCT 
TTATTTGTAC AGTTGATCCT GGAAAATATA TGGAGTTTGT ATGATGCTGT TTTGAAAAAG 
GACAAAGACA AAATTGATAA AATAGTGACT TCTTTAGGAT TAAAAATTGG AGCCCGGGAG 
GCACGACATT CAGACCCTAA AGTTCAGATC AACGCCATTT GCAGTCAGTG GCTACCCATA 
TCCCATGCTG TTCTTGCTAT GGTGTGTCAG AAACTTCCTA GTCCCCTTGA TATTACAGCT 
GAGAGAGTGG AGAGACTGAT GTGCACAGGA TCACAAACTT TTGACTCTTT TCCACCAGAA 
ACTCAAGCAC TGAAAGCAGC TTTTATGAAA TGTGGAAGTG AGGACACTGC TCCAGTTATT 
ATATTTGTTT CCAAAATGTT TGCAGTTGAT GCTAAGGCCT TGCCTCAGAA TAAGCCAAGG 
CCTCTCACTC AAGAAGAAAT TGCTCAGAGA CGTGAGCGTG CAAGACAAAG GCATGCAGAG 
AAGCTTGCAG CAGCACAGGG ACAGGCACCC TTGGAGCCCA CCCAAGATGG GAGTGCCATT 
GAAACATGTC CAAAAGGAGA GGAGCCAAGA GGTGACGAGC AACAGGTGGA AAGTATGACC 
CCTAAACCTG TGCTCCAGGA AGAAAACAAC CAAGAGTCTT TTATTGCATT TGCTCGGGTG 
TTCAGTGGTG TGGCTCGAAG AGGAAAGAAA ATTTTTGTCT TGGGGCCCAA ATACAGTCCT 
CTTGAGTTTT TACGAAGGGT ACCATTAGGC TTCTCAGCTC CACCAGATGG CCTCCCCCAA 
GTCCCCCACA TGGCATACTG TGCTCTGGAA AACCTGTATC TTCTGATGGG AAGGGAACTG 
GAATATCTAG AGGAGGTACC TCCAGGAAAT GTGCTAGGAA TAGGAGGCCT TCAAGATTTT 
GTGCTGAAAT CTGCAACACT GTGTAGCCTG CCATCCTGCC CACCATTTAT ACCACTCAAC 
TTCGAAGCCA CTCCTATTGT GAGAGTTGCT GTTGAACCAA AACATCCAAG TGAAATGCCT 
CAGCTCGTAA AAGGAATGAA ACTGTTAAAC CAGGCTGATC CCTGTGTCCA GATTTTAATT 
CAGGAAACGG GAGAGCACGT TTTAGTCACA GCAGGAGAAG TCCACCTTCA GCGATGCCTG 
GATGACTTAA AAGAAAGGTT TGCAAAGATT CATATCAGTG TATCTGAACC TATTATTCCA 
TTCAGAGAAA CAATCACAAA ACCCCCAAAA GTTGACATGG TCAATGAAGA AATAGGCAAA 
CAGCAAAAAG TTGCAGTCAT ACACCAAATG AAAGAAGATC AAAGCAAAAT CCCTGAAGGA 
ATCCAAGTTG ACTCTGACGG GCTAATCACC ATAACAACTC CCAATAAACT TGCCACGCTC 
AGTGTTCGAG CCATGCCCCT TCCAGAAGAA GTCACCCAGA TTCTGGAAGA AAATAGTGAT 
TTGATTCGTT CTATGGAGCA GTTGACATCC TCTTTGAATG AGGGTGAAAA TACTCACATG 
ATTCATCAGA AGACCCAAGA GAAAATTTGG GAATTCAAAG GAAAACTGGA GCAACACCTA 
ACAGGGAGAA GATGGAGGAA CATTGTTGAC CAAATCTGGT CATTTGGCCC AAGAAAATGT 
GGGCCCAACA TACTAGTCAA TAAAAGTGAA GATTTTCAGA ACTCAGTATG GACAGGTCCA 
GCTGACAAAG CTTCAAAAGA AGCCAGTAGA TACCGAGATT TGGGCAATAG CATTGTGAGT 
GGCTTCCAAC TAGCAACCCT CTCTGGCCCC ATGTGTGAGG AGCCTCTCAT GGGTGTCTGT 
TTTGTTCTGG AAAAATGGGA CCTAAGTAAA TTTGAGGAAC AAGGAGCAAG TGATCTGGCA 
AAAGAGGGAC AGGAGGAAAA TGAAACCTGT TCTGGTGGAA ATGAAAACCA AGAGCTACAA 
GATGGCTGCT CTGAGGCCTT TGAGAAGAGG ACATCACAGA AAGGAGAATC TCCACTCACT 
GACTGCTATG GACCTTTCTC AGGACAGCTA ATTGCCACCA TGAAAGAAGC ATGTCGCTAT 
GCACTGCAAG TGAAACCTCA GCGCCTGATG GCAGCTATGT ACACATGTGA CATCATGGCC 
ACTGGTGATG TTCTCGGTCG AGTCTATGCT GTCTTGTCAA AGAGAGAAGG TCGGGTACTT 
CAAGAAGAAA TGAAAGAAGG GACAGACATG TTCATCATCA AGGCTGTGCT GCCTGTTGCT 
GAAAGCTTTG GTTTTGCTGA TGAAATCAGG AAGAGGACAA GTGGCCTGGC CAGCCCACAA 
CTAGTATTCA GCCATTGGGA GATCATTCCC AGTGACCCCT TCTGGGTGCC AACTACTGAG 
GAGGAATACT TGCACTTTGG GGAGAAGGCT GACTCTGAGA ACCAAGCCCG GAAGTACATG 
AACGCAGTAC GAAAGCGGAA GGGGCTTTAT GTGGAAGAAA AGATTGTGGA GCATGCAGAA 
AAGCAGAGGA CACTCAGCAA AAATAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035700.1
CDS	170..3379
Translation	MVLNSLDKMIQLQKNTANIRNICVLAHVDHGNEEYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAERETESQVNPNSEQGEQVYDWSTGLEDTDDSHLYFSPEQGNVVFTSAIDGWGFGIEHFARIYSQKIGIKKEVLMKTLWGDYYINMKAKKIMKGDQAKGKKPLFVQLILENIWSLYDAVLKKDKDKIDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISHAVLAMVCQKLPSPLDITAERVERLMCTGSQTFDSFPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAQRRERARQRHAEKLAAAQGQAPLEPTQDGSAIETCPKGEEPRGDEQQVESMTPKPVLQEENNQESFIAFARVFSGVARRGKKIFVLGPKYSPLEFLRRVPLGFSAPPDGLPQVPHMAYCALENLYLLMGRELEYLEEVPPGNVLGIGGLQDFVLKSATLCSLPSCPPFIPLNFEATPIVRVAVEPKHPSEMPQLVKGMKLLNQADPCVQILIQETGEHVLVTAGEVHLQRCLDDLKERFAKIHISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQMKEDQSKIPEGIQVDSDGLITITTPNKLATLSVRAMPLPEEVTQILEENSDLIRSMEQLTSSLNEGENTHMIHQKTQEKIWEFKGKLEQHLTGRRWRNIVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPADKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDLAKEGQEENETCSGGNENQELQDGCSEAFEKRTSQKGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	NM_001040610.2
Organism	Homo sapiens(human)
Definition	Homo sapiens elongation factor like GTPase 1 (EFL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040610.2

ORF Insert Sequence:

ATGGTGCTCA ACAGTTTGGA TAAGATGATT CAACTCCAGA AAAACACTGC CAACATCAGG 
AATATTTGTG TTTTGGCTCA TGTTGACCAT GGTAATGAGG AGTACCTGAT CAATCTGATA 
GACTCTCCAG GACACGTGGA CTTTTCCTCA GAAGTATCAA CCGCTGTTCG CATTTGTGAT 
GGATGCATCA TTGTGGTAGA TGCTGTGGAA GGAGTCTGTC CACAGACACA GGCAGTTCTG 
CGACAAGCTT GGCTTGAAAA CATCCGTCCG GTTTTAGTGA TTAATAAGAT TGATCGCTTG 
ATAGTGGAAC TGAAATTCAC CCCACAAGAG GCCTATTCTC ACCTCAAGAA TATTTTAGAA 
CAGATTAATG CGCTCACAGG GACTCTTTTT ACTTCTAAAG TCCTAGAAGA AAGAGCAGAG 
AGGGAGACTG AATCCCAAGT GAATCCAAAT TCTGAACAAG GAGAGCAAGT ATATGACTGG 
AGCACTGGCT TGGAGGACAC AGATGATTCT CACCTTTACT TCTCTCCAGA ACAGGGAAAT 
GTGGTGTTTA CCAGTGCAAT AGATGGGTGG GGCTTTGGAA TTGAGCACTT CGCCAGAATC 
TACAGTCAAA AAATTGGCAT CAAAAAGGAA GTTCTTATGA AAACCTTGTG GGGAGATTAC 
TATATAAATA TGAAGGCTAA AAAGATCATG AAGGGTGATC AGGCCAAAGG AAAGAAACCT 
TTATTTGTAC AGTTGATCCT GGAAAATATA TGGAGTTTGT ATGATGCTGT TTTGAAAAAG 
GACAAAGACA AAATTGATAA AATAGTGACT TCTTTAGGAT TAAAAATTGG AGCCCGGGAG 
GCACGACATT CAGACCCTAA AGTTCAGATC AACGCCATTT GCAGTCAGTG GCTACCCATA 
TCCCATGCTG TTCTTGCTAT GGTGTGTCAG AAACTTCCTA GTCCCCTTGA TATTACAGCT 
GAGAGAGTGG AGAGACTGAT GTGCACAGGA TCACAAACTT TTGACTCTTT TCCACCAGAA 
ACTCAAGCAC TGAAAGCAGC TTTTATGAAA TGTGGAAGTG AGGACACTGC TCCAGTTATT 
ATATTTGTTT CCAAAATGTT TGCAGTTGAT GCTAAGGCCT TGCCTCAGAA TAAGCCAAGG 
CCTCTCACTC AAGAAGAAAT TGCTCAGAGA CGTGAGCGTG CAAGACAAAG GCATGCAGAG 
AAGCTTGCAG CAGCACAGGG ACAGGCACCC TTGGAGCCCA CCCAAGATGG GAGTGCCATT 
GAAACATGTC CAAAAGGAGA GGAGCCAAGA GGTGACGAGC AACAGGTGGA AAGTATGACC 
CCTAAACCTG TGCTCCAGGA AGAAAACAAC CAAGAGTCTT TTATTGCATT TGCTCGGGTG 
TTCAGTGGTG TGGCTCGAAG AGGAAAGAAA ATTTTTGTCT TGGGGCCCAA ATACAGTCCT 
CTTGAGTTTT TACGAAGGGT ACCATTAGGC TTCTCAGCTC CACCAGATGG CCTCCCCCAA 
GTCCCCCACA TGGCATACTG TGCTCTGGAA AACCTGTATC TTCTGATGGG AAGGGAACTG 
GAATATCTAG AGGAGGTACC TCCAGGAAAT GTGCTAGGAA TAGGAGGCCT TCAAGATTTT 
GTGCTGAAAT CTGCAACACT GTGTAGCCTG CCATCCTGCC CACCATTTAT ACCACTCAAC 
TTCGAAGCCA CTCCTATTGT GAGAGTTGCT GTTGAACCAA AACATCCAAG TGAAATGCCT 
CAGCTCGTAA AAGGAATGAA ACTGTTAAAC CAGGCTGATC CCTGTGTCCA GATTTTAATT 
CAGGAAACGG GAGAGCACGT TTTAGTCACA GCAGGAGAAG TCCACCTTCA GCGATGCCTG 
GATGACTTAA AAGAAAGGTT TGCAAAGATT CATATCAGTG TATCTGAACC TATTATTCCA 
TTCAGAGAAA CAATCACAAA ACCCCCAAAA GTTGACATGG TCAATGAAGA AATAGGCAAA 
CAGCAAAAAG TTGCAGTCAT ACACCAAATG AAAGAAGATC AAAGCAAAAT CCCTGAAGGA 
ATCCAAGTTG ACTCTGACGG GCTAATCACC ATAACAACTC CCAATAAACT TGCCACGCTC 
AGTGTTCGAG CCATGCCCCT TCCAGAAGAA GTCACCCAGA TTCTGGAAGA AAATAGTGAT 
TTGATTCGTT CTATGGAGCA GTTGACATCC TCTTTGAATG AGGGTGAAAA TACTCACATG 
ATTCATCAGA AGACCCAAGA GAAAATTTGG GAATTCAAAG GAAAACTGGA GCAACACCTA 
ACAGGGAGAA GATGGAGGAA CATTGTTGAC CAAATCTGGT CATTTGGCCC AAGAAAATGT 
GGGCCCAACA TACTAGTCAA TAAAAGTGAA GATTTTCAGA ACTCAGTATG GACAGGTCCA 
GCTGACAAAG CTTCAAAAGA AGCCAGTAGA TACCGAGATT TGGGCAATAG CATTGTGAGT 
GGCTTCCAAC TAGCAACCCT CTCTGGCCCC ATGTGTGAGG AGCCTCTCAT GGGTGTCTGT 
TTTGTTCTGG AAAAATGGGA CCTAAGTAAA TTTGAGGAAC AAGGAGCAAG TGATCTGGCA 
AAAGAGGGAC AGGAGGAAAA TGAAACCTGT TCTGGTGGAA ATGAAAACCA AGAGCTACAA 
GATGGCTGCT CTGAGGCCTT TGAGAAGAGG ACATCACAGA AAGGAGAATC TCCACTCACT 
GACTGCTATG GACCTTTCTC AGGACAGCTA ATTGCCACCA TGAAAGAAGC ATGTCGCTAT 
GCACTGCAAG TGAAACCTCA GCGCCTGATG GCAGCTATGT ACACATGTGA CATCATGGCC 
ACTGGTGATG TTCTCGGTCG AGTCTATGCT GTCTTGTCAA AGAGAGAAGG TCGGGTACTT 
CAAGAAGAAA TGAAAGAAGG GACAGACATG TTCATCATCA AGGCTGTGCT GCCTGTTGCT 
GAAAGCTTTG GTTTTGCTGA TGAAATCAGG AAGAGGACAA GTGGCCTGGC CAGCCCACAA 
CTAGTATTCA GCCATTGGGA GATCATTCCC AGTGACCCCT TCTGGGTGCC AACTACTGAG 
GAGGAATACT TGCACTTTGG GGAGAAGGCT GACTCTGAGA ACCAAGCCCG GAAGTACATG 
AACGCAGTAC GAAAGCGGAA GGGGCTTTAT GTGGAAGAAA AGATTGTGGA GCATGCAGAA 
AAGCAGAGGA CACTCAGCAA AAATAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in
Journal of medical genetics54(8)558-566(2017 Aug)
Stepensky P,Chac?n-Flores M,Kim KH,Abuzaitoun O,Bautista-Santos A,Simanovsky N,Siliqi D,Altamura D,M?ndez-Godoy A,Gijsbers A,Naser Eddin A,Dor T,Charrow J,S?nchez-Puig N,Elpeleg O

Mechanism of eIF6 release from the nascent 60S ribosomal subunit.
Nature structural & molecular biology22(11)914-9(2015 Nov)
Weis F,Giudice E,Churcher M,Jin L,Hilcenko C,Wong CC,Traynor D,Kay RR,Warren AJ

Defective Guanine Nucleotide Exchange in the Elongation Factor-like 1 (EFL1) GTPase by Mutations in the Shwachman-Diamond Syndrome Protein.
The Journal of biological chemistry290(29)17669-78(2015 Jul)
Garc?a-M?rquez A,Gijsbers A,de la Mora E,S?nchez-Puig N

Functional analysis of a novel glioma antigen, EFTUD1.
Neuro-oncology16(12)1618-29(2014 Dec)
Saito K,Iizuka Y,Ohta S,Takahashi S,Nakamura K,Saya H,Yoshida K,Kawakami Y,Toda M

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EFL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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