NCU06278 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU06278
Entrez Gene ID	3879021
Full Name	elongation factor 2
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395605.1	XP_011393907.1	elongation factor 2
XM_011395606.1	XP_011393908.1	elongation factor 2, variant 1
XM_011395607.1	XP_011393909.1	elongation factor 2, variant 2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG33158	NP_788515.1
Schizosaccharomyces pombe (fission yeast)	SPCC553.08c	NP_587766.1
Magnaporthe oryzae (rice blast fungus)	MGG_05265	XP_003712823.1
Arabidopsis thaliana (thale cress)	AT3G22980	NP_188938.1
Oryza sativa (rice)	Os03g0650700	NP_001050786.1
Bos taurus (cattle)	EFTUD1	XP_002696652.1
Danio rerio (zebrafish)	eftud1	XP_697184.5
Saccharomyces cerevisiae (baker's yeast)	RIA1	NP_014236.1
Eremothecium gossypii	AGOS_AFR031C	NP_985578.2
Pan troglodytes (chimpanzee)	EFTUD1	XP_510546.2
	EFTUD1	XP_002804960.1
Mus musculus (house mouse)	Eftud1	NP_780526.2
Rattus norvegicus (Norway rat)	Eftud1	NP_001101004.1
Gallus gallus (chicken)	EFTUD1	NP_001034345.2
Homo sapiens (human)	EFTUD1	NP_078856.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003001	XP_311881.3
Kluyveromyces lactis	KLLA0D07898g	XP_453415.1
Oryza sativa (rice)	Os02g0543300	NP_001047074.1
Canis lupus familiaris (dog)	EFTUD1	XP_850111.1
Neurospora crassa	NCU06278	XP_962882.2
Xenopus tropicalis (tropical clawed frog)	eftud1	XP_002940146.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU06278 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU06278 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONf12276	XM_011395607.1 Latest version!	Neurospora crassa OR74A elongation factor 2, variant 2 (NCU06278), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ONf12276	XM_011395606.1 Latest version!	Neurospora crassa OR74A elongation factor 2, variant 1 (NCU06278), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ONf12276	XM_011395605.1 Latest version!	Neurospora crassa OR74A elongation factor 2 (NCU06278), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU06278 ( XM_011395605.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393907.1 Neurospora crassa OR74A elongation factor 2 (NCU06278), mRNA.

Price & Availability↑

CloneID	ONf12276
Clone ID Related Accession (Same CDS sequence)	XM_011395605.1 , XM_011395606.1 , XM_011395607.1
Accession Version	XM_011395605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	elongation factor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503).

ATGCCTGTTG TCCAGCCAGA AAAGCTGGCC AAGCTCCAAC AAAATGCCGA CGACGTGAGG 
AATATTTGTA TCTTGGCTCA CGTTGACCAT GGGAAAACAT CACTCACAGA TGCCCTTCTC 
GCCACCAATG GCATCATCTC ACCAAAACTC GCCGGAAAGA TTCGCTATCT CGATTCCCGG 
CCGGATGAAC AGCAAAGAGG TATCACCATG GAGTCTTCAG CCATTTCACT ATACTTCTCC 
ATGTTGAGGA GGTCGGCTCC TGACGCTACT CCCGAGGCCA AGGAGTACTT GATCAACCTG 
ATCGACTCCC CCGGACATAT CGATTTCAGC AGTGAAGTGT CCACTGCTTC TCGCCTTTGT 
GACGGTGCCG TTGTTCTGGT CGATGCTGTC GAAGGCGTCT GCAGTCAGAC AGTAACCGTG 
TTGCGCCAAG CATGGACCGA GAAGCTCAAG CCCCTTCTTG TTATCAACAA AATCGACCGC 
TTGGTTACCG AGTTGAAGAT GACCCCGGGC GAGGCTTATA TCCACCTCAG CAAAATTCTC 
GAGCAAGTCA ACGCAGTTCT TGGAAGCTTC TTCCAAGGTG AGCGCATGGA AGAAGATCTC 
AACTGGCGTG ACCGGATGGA GGAAAGAGTG GCTGCCGCCG CGGCCAAGGA GGCTCAAATA 
GCTGCCGGTC AACCTGACTC CGGCGAGCTT CAGTTCCAGG AAAAAGACGA CGAGGAGATT 
TACTTTGCGC CAGAGAAGAA CAACGTTATT TTTGGCAGTG CCATCGACGG CTGGGCTTTT 
ACTGTTCGCC AGTTTGCCGG CATGTACGAG AAGAAGCTCG GCATCAAGCG TGGCTTGCTG 
GAAAAGGTTC TCTGGGGCAA CTTCTACATG GATCCCAAGA CCAAAAAGGT ACTTGGGCCT 
AAGCATCTTA AGGGCAGACC TCTAAAGCCC ATCTTTGTCC AGTTGGTTCT GGAGCCAATT 
TGGGCTGTCT ACCAGGCCAC GGTTGGTGGT GACTCCGGTA AGGGTGATCC CGCTCTTTTG 
GAGAAGATCA CCAAGTCCTT GAACCTCAGT GTACCGCCCC ACATCCTCCG CTCTCGGGAT 
CCCAAACTAC TCCTTACCAC GGTGTTTGCT TCTTGGTTGC CTCTCTCTAC GGCTTTGCTC 
GTCTCAGTGG TGGAGTCATT GCCTTCGCCC AAAGCAGCTC AGGCGGACCG TCTCCCAGAC 
CTCCTGGCAA GCGTACCCGG CGCGGACCAC ATCGATCCCA AGGTCAAAGA TGCCATGGTC 
TCGTTCAAAA AAGATCCATC TGAGCCTATG GTTGCATATG TTAGCAAGAT GATATCCGTG 
CCTGAAAGCG AGCTGCCGGA GAACAGGCGC CGCGGACCGC TTAGCCCTGA GGAGGCTCGA 
GAGCTGGCGA GAAAGAAGCG CGCCGAAGCC ATTCGTGCCC AAGGAGGCGC CGACGGTGAC 
CCTGACATGA ACGACCTGGC CAACGCCTTC ACCAGCACCT CCTTGACCGA CAATGCCGTG 
CCGGAACTCG AGGAGAAGCC CGTCGAAGCG GAGCATCTCA TCGGTTTTGC CCGTATCTAC 
TCGGGCACTC TATCCGTTGG CGACGAGATC TATGTGTTGC CCCCAAAGTT CTCCCCGGCT 
GATCCTCATG CCCAACCCGT CCCGAAGAAG GTGACGGTTA CCGCTCTCTA CATGCTCATG 
GGTCGCAATC TCGAAGCCCT TCCCTCCGTC CCAGCTGGTG TCGTGTTCGG TATTCGCGGT 
CTCGAAGGCA GCGGCCTTCT CAAGTCCGGA ACGCTCTGCA GCCAGCTCGA GGGCTCGGTC 
AACTTGGCTG GTATCGCCAA CCTGGCCGGT CGCCCCATTG TTCGTGTCGC TCTTGAGCCT 
GTCAACCCCT ATGACCTGGA CAAGATGATT CAGGGTCTTC ACCTCCTTGT CCAGAGCGAT 
CCGTGCGCTG AGTATGAGCA CTTCTCCAGT GGCGAGCACG TCCTCTCTAC TGCCGGCGAA 
TTGCATCTCG AACGCTGCCT GACGGATCTG CGCGAGCGGT TTGCGCGATG CGAGATCCAA 
GCCGGTGCGC CCATTGTGCC GTACCGGGAG ACGATCGTTC GTGCCGAAGA GATGCGCCCT 
CCAGTGAACA AGGAACTCGG CCGCGGTGTG GTTGTCGGCG TCACGAGCTC CAGGCAAGTG 
ACGGTGACCC TGCGCGTCAG GCCCCTCCCG GCCCCCGTAA CTGAATTCCT CCAGAAGAAC 
TCCGCCGGCA TCAAGCGGCT GTACACGGAC CGCAAGGCGG CTGGCGTCGA GGACGGCACC 
GAGTCACCCT CTGTAGAAGA GGCTACGTTG GAGAACGGCA CCCCGTCGGA CGTCAAGATT 
GAAGAAGACG AGGAGGTGTC CCAAGCCGCC ACCACCCTCA CGCCCGAGGA GCTCAAGAAG 
CAGCTCCAGG CCCAGCTCGA CGGCGTCAAA GACAAGGAGG CTTGGAAGGA CGTGATCGAC 
CGCATCGCCT CTTTTGGCCC GCGCAGAACG GGCGCCAACA TTCTCGTCGA CGCCACCAAG 
GACCAGCTCT TCAGCAAGGC CTTTTCGGCC GACAAGATCC GCAATCAGGC GCCCACGGGC 
GACGAGAAGC TGCACCCAGC CCACTTCAGC GATAAGATCA TGTACGGTTT CCAGCTGGCC 
ACGCAGCAAG GGCCGCTTTG CAACGAGCCC GTGCAGGGCA TCGCCGTCTT CGTGGAGGAT 
GTTCTGGTGG CGCAGACAGA GGACGACGAG GCCTCGTCGG CGCGTGACAG GATCGGCCGC 
TTGACGGGTG AGGTTATCAA GACGGTGCAG CAGTCGATTC ACAAGGGATT CTTGGACTGG 
TCGCCGCGCT TGATGTTGGC CATGTACTCG TGCGAGATTC AAGCGAGCAC CGAAGTCCTA 
GGCCGTGTCT ACGACGTGCT CACCCGCCGC CGCGGCCGCG TGCAGTCGGA AGCCATGAAC 
GAAGGCACGC CCTTTTTCAC TATCCAGGCC CTGCTGCCCG TGGCCGAGTC GTTCGGGTTC 
GCCGACGACA TGCGCAAGCG CACCTCGGGC GCCGCGCAGC CGCAGCTGAT CTTCACGGGT 
TACGAGATCC TGGACGAGGA TCCCTTCTGG GTGCCCTTCA CCGAAGACGA CTTGGAGGAC 
CTGGGCGAGT TTGGCGACAG GGAGAATGTG GCCAAGAGGT ATATGGATAG TGTGCGCAGG 
AGAAAGGGGT TGTTGGTGGA AGGGAGGAAT GTGGCTACTA ATGCGGAAAA GCAAAAGACT 
TTGAAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393907.1
CDS	347..3598
Translation	MPVVQPEKLAKLQQNADDVRNICILAHVDHGKTSLTDALLATNGIISPKLAGKIRYLDSRPDEQQRGITMESSAISLYFSMLRRSAPDATPEAKEYLINLIDSPGHIDFSSEVSTASRLCDGAVVLVDAVEGVCSQTVTVLRQAWTEKLKPLLVINKIDRLVTELKMTPGEAYIHLSKILEQVNAVLGSFFQGERMEEDLNWRDRMEERVAAAAAKEAQIAAGQPDSGELQFQEKDDEEIYFAPEKNNVIFGSAIDGWAFTVRQFAGMYEKKLGIKRGLLEKVLWGNFYMDPKTKKVLGPKHLKGRPLKPIFVQLVLEPIWAVYQATVGGDSGKGDPALLEKITKSLNLSVPPHILRSRDPKLLLTTVFASWLPLSTALLVSVVESLPSPKAAQADRLPDLLASVPGADHIDPKVKDAMVSFKKDPSEPMVAYVSKMISVPESELPENRRRGPLSPEEARELARKKRAEAIRAQGGADGDPDMNDLANAFTSTSLTDNAVPELEEKPVEAEHLIGFARIYSGTLSVGDEIYVLPPKFSPADPHAQPVPKKVTVTALYMLMGRNLEALPSVPAGVVFGIRGLEGSGLLKSGTLCSQLEGSVNLAGIANLAGRPIVRVALEPVNPYDLDKMIQGLHLLVQSDPCAEYEHFSSGEHVLSTAGELHLERCLTDLRERFARCEIQAGAPIVPYRETIVRAEEMRPPVNKELGRGVVVGVTSSRQVTVTLRVRPLPAPVTEFLQKNSAGIKRLYTDRKAAGVEDGTESPSVEEATLENGTPSDVKIEEDEEVSQAATTLTPEELKKQLQAQLDGVKDKEAWKDVIDRIASFGPRRTGANILVDATKDQLFSKAFSADKIRNQAPTGDEKLHPAHFSDKIMYGFQLATQQGPLCNEPVQGIAVFVEDVLVAQTEDDEASSARDRIGRLTGEVIKTVQQSIHKGFLDWSPRLMLAMYSCEIQASTEVLGRVYDVLTRRRGRVQSEAMNEGTPFFTIQALLPVAESFGFADDMRKRTSGAAQPQLIFTGYEILDEDPFWVPFTEDDLEDLGEFGDRENVAKRYMDSVRRRKGLLVEGRNVATNAEKQKTLKR

Target ORF information:

RefSeq Version	XM_011395605.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A elongation factor 2 (NCU06278), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395605.1

ORF Insert Sequence:

ATGCCTGTTG TCCAGCCAGA AAAGCTGGCC AAGCTCCAAC AAAATGCCGA CGACGTGAGG 
AATATTTGTA TCTTGGCTCA CGTTGACCAT GGGAAAACAT CACTCACAGA TGCCCTTCTC 
GCCACCAATG GCATCATCTC ACCAAAACTC GCCGGAAAGA TTCGCTATCT CGATTCCCGG 
CCGGATGAAC AGCAAAGAGG TATCACCATG GAGTCTTCAG CCATTTCACT ATACTTCTCC 
ATGTTGAGGA GGTCGGCTCC TGACGCTACT CCCGAGGCCA AGGAGTACTT GATCAACCTG 
ATCGACTCCC CCGGACATAT CGATTTCAGC AGTGAAGTGT CCACTGCTTC TCGCCTTTGT 
GACGGTGCCG TTGTTCTGGT CGATGCTGTC GAAGGCGTCT GCAGTCAGAC AGTAACCGTG 
TTGCGCCAAG CATGGACCGA GAAGCTCAAG CCCCTTCTTG TTATCAACAA AATCGACCGC 
TTGGTTACCG AGTTGAAGAT GACCCCGGGC GAGGCTTATA TCCACCTCAG CAAAATTCTC 
GAGCAAGTCA ACGCAGTTCT TGGAAGCTTC TTCCAAGGTG AGCGCATGGA AGAAGATCTC 
AACTGGCGTG ACCGGATGGA GGAAAGAGTG GCTGCCGCCG CGGCCAAGGA GGCTCAAATA 
GCTGCCGGTC AACCTGACTC CGGCGAGCTT CAGTTCCAGG AAAAAGACGA CGAGGAGATT 
TACTTTGCGC CAGAGAAGAA CAACGTTATT TTTGGCAGTG CCATCGACGG CTGGGCTTTT 
ACTGTTCGCC AGTTTGCCGG CATGTACGAG AAGAAGCTCG GCATCAAGCG TGGCTTGCTG 
GAAAAGGTTC TCTGGGGCAA CTTCTACATG GATCCCAAGA CCAAAAAGGT ACTTGGGCCT 
AAGCATCTTA AGGGCAGACC TCTAAAGCCC ATCTTTGTCC AGTTGGTTCT GGAGCCAATT 
TGGGCTGTCT ACCAGGCCAC GGTTGGTGGT GACTCCGGTA AGGGTGATCC CGCTCTTTTG 
GAGAAGATCA CCAAGTCCTT GAACCTCAGT GTACCGCCCC ACATCCTCCG CTCTCGGGAT 
CCCAAACTAC TCCTTACCAC GGTGTTTGCT TCTTGGTTGC CTCTCTCTAC GGCTTTGCTC 
GTCTCAGTGG TGGAGTCATT GCCTTCGCCC AAAGCAGCTC AGGCGGACCG TCTCCCAGAC 
CTCCTGGCAA GCGTACCCGG CGCGGACCAC ATCGATCCCA AGGTCAAAGA TGCCATGGTC 
TCGTTCAAAA AAGATCCATC TGAGCCTATG GTTGCATATG TTAGCAAGAT GATATCCGTG 
CCTGAAAGCG AGCTGCCGGA GAACAGGCGC CGCGGACCGC TTAGCCCTGA GGAGGCTCGA 
GAGCTGGCGA GAAAGAAGCG CGCCGAAGCC ATTCGTGCCC AAGGAGGCGC CGACGGTGAC 
CCTGACATGA ACGACCTGGC CAACGCCTTC ACCAGCACCT CCTTGACCGA CAATGCCGTG 
CCGGAACTCG AGGAGAAGCC CGTCGAAGCG GAGCATCTCA TCGGTTTTGC CCGTATCTAC 
TCGGGCACTC TATCCGTTGG CGACGAGATC TATGTGTTGC CCCCAAAGTT CTCCCCGGCT 
GATCCTCATG CCCAACCCGT CCCGAAGAAG GTGACGGTTA CCGCTCTCTA CATGCTCATG 
GGTCGCAATC TCGAAGCCCT TCCCTCCGTC CCAGCTGGTG TCGTGTTCGG TATTCGCGGT 
CTCGAAGGCA GCGGCCTTCT CAAGTCCGGA ACGCTCTGCA GCCAGCTCGA GGGCTCGGTC 
AACTTGGCTG GTATCGCCAA CCTGGCCGGT CGCCCCATTG TTCGTGTCGC TCTTGAGCCT 
GTCAACCCCT ATGACCTGGA CAAGATGATT CAGGGTCTTC ACCTCCTTGT CCAGAGCGAT 
CCGTGCGCTG AGTATGAGCA CTTCTCCAGT GGCGAGCACG TCCTCTCTAC TGCCGGCGAA 
TTGCATCTCG AACGCTGCCT GACGGATCTG CGCGAGCGGT TTGCGCGATG CGAGATCCAA 
GCCGGTGCGC CCATTGTGCC GTACCGGGAG ACGATCGTTC GTGCCGAAGA GATGCGCCCT 
CCAGTGAACA AGGAACTCGG CCGCGGTGTG GTTGTCGGCG TCACGAGCTC CAGGCAAGTG 
ACGGTGACCC TGCGCGTCAG GCCCCTCCCG GCCCCCGTAA CTGAATTCCT CCAGAAGAAC 
TCCGCCGGCA TCAAGCGGCT GTACACGGAC CGCAAGGCGG CTGGCGTCGA GGACGGCACC 
GAGTCACCCT CTGTAGAAGA GGCTACGTTG GAGAACGGCA CCCCGTCGGA CGTCAAGATT 
GAAGAAGACG AGGAGGTGTC CCAAGCCGCC ACCACCCTCA CGCCCGAGGA GCTCAAGAAG 
CAGCTCCAGG CCCAGCTCGA CGGCGTCAAA GACAAGGAGG CTTGGAAGGA CGTGATCGAC 
CGCATCGCCT CTTTTGGCCC GCGCAGAACG GGCGCCAACA TTCTCGTCGA CGCCACCAAG 
GACCAGCTCT TCAGCAAGGC CTTTTCGGCC GACAAGATCC GCAATCAGGC GCCCACGGGC 
GACGAGAAGC TGCACCCAGC CCACTTCAGC GATAAGATCA TGTACGGTTT CCAGCTGGCC 
ACGCAGCAAG GGCCGCTTTG CAACGAGCCC GTGCAGGGCA TCGCCGTCTT CGTGGAGGAT 
GTTCTGGTGG CGCAGACAGA GGACGACGAG GCCTCGTCGG CGCGTGACAG GATCGGCCGC 
TTGACGGGTG AGGTTATCAA GACGGTGCAG CAGTCGATTC ACAAGGGATT CTTGGACTGG 
TCGCCGCGCT TGATGTTGGC CATGTACTCG TGCGAGATTC AAGCGAGCAC CGAAGTCCTA 
GGCCGTGTCT ACGACGTGCT CACCCGCCGC CGCGGCCGCG TGCAGTCGGA AGCCATGAAC 
GAAGGCACGC CCTTTTTCAC TATCCAGGCC CTGCTGCCCG TGGCCGAGTC GTTCGGGTTC 
GCCGACGACA TGCGCAAGCG CACCTCGGGC GCCGCGCAGC CGCAGCTGAT CTTCACGGGT 
TACGAGATCC TGGACGAGGA TCCCTTCTGG GTGCCCTTCA CCGAAGACGA CTTGGAGGAC 
CTGGGCGAGT TTGGCGACAG GGAGAATGTG GCCAAGAGGT ATATGGATAG TGTGCGCAGG 
AGAAAGGGGT TGTTGGTGGA AGGGAGGAAT GTGGCTACTA ATGCGGAAAA GCAAAAGACT 
TTGAAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU06278 ( XM_011395606.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393908.1 Neurospora crassa OR74A elongation factor 2, variant 1 (NCU06278), mRNA.

Price & Availability↑

CloneID	ONf12276
Clone ID Related Accession (Same CDS sequence)	XM_011395605.1 , XM_011395606.1 , XM_011395607.1
Accession Version	XM_011395606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	elongation factor 2, variant 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503).

ATGCCTGTTG TCCAGCCAGA AAAGCTGGCC AAGCTCCAAC AAAATGCCGA CGACGTGAGG 
AATATTTGTA TCTTGGCTCA CGTTGACCAT GGGAAAACAT CACTCACAGA TGCCCTTCTC 
GCCACCAATG GCATCATCTC ACCAAAACTC GCCGGAAAGA TTCGCTATCT CGATTCCCGG 
CCGGATGAAC AGCAAAGAGG TATCACCATG GAGTCTTCAG CCATTTCACT ATACTTCTCC 
ATGTTGAGGA GGTCGGCTCC TGACGCTACT CCCGAGGCCA AGGAGTACTT GATCAACCTG 
ATCGACTCCC CCGGACATAT CGATTTCAGC AGTGAAGTGT CCACTGCTTC TCGCCTTTGT 
GACGGTGCCG TTGTTCTGGT CGATGCTGTC GAAGGCGTCT GCAGTCAGAC AGTAACCGTG 
TTGCGCCAAG CATGGACCGA GAAGCTCAAG CCCCTTCTTG TTATCAACAA AATCGACCGC 
TTGGTTACCG AGTTGAAGAT GACCCCGGGC GAGGCTTATA TCCACCTCAG CAAAATTCTC 
GAGCAAGTCA ACGCAGTTCT TGGAAGCTTC TTCCAAGGTG AGCGCATGGA AGAAGATCTC 
AACTGGCGTG ACCGGATGGA GGAAAGAGTG GCTGCCGCCG CGGCCAAGGA GGCTCAAATA 
GCTGCCGGTC AACCTGACTC CGGCGAGCTT CAGTTCCAGG AAAAAGACGA CGAGGAGATT 
TACTTTGCGC CAGAGAAGAA CAACGTTATT TTTGGCAGTG CCATCGACGG CTGGGCTTTT 
ACTGTTCGCC AGTTTGCCGG CATGTACGAG AAGAAGCTCG GCATCAAGCG TGGCTTGCTG 
GAAAAGGTTC TCTGGGGCAA CTTCTACATG GATCCCAAGA CCAAAAAGGT ACTTGGGCCT 
AAGCATCTTA AGGGCAGACC TCTAAAGCCC ATCTTTGTCC AGTTGGTTCT GGAGCCAATT 
TGGGCTGTCT ACCAGGCCAC GGTTGGTGGT GACTCCGGTA AGGGTGATCC CGCTCTTTTG 
GAGAAGATCA CCAAGTCCTT GAACCTCAGT GTACCGCCCC ACATCCTCCG CTCTCGGGAT 
CCCAAACTAC TCCTTACCAC GGTGTTTGCT TCTTGGTTGC CTCTCTCTAC GGCTTTGCTC 
GTCTCAGTGG TGGAGTCATT GCCTTCGCCC AAAGCAGCTC AGGCGGACCG TCTCCCAGAC 
CTCCTGGCAA GCGTACCCGG CGCGGACCAC ATCGATCCCA AGGTCAAAGA TGCCATGGTC 
TCGTTCAAAA AAGATCCATC TGAGCCTATG GTTGCATATG TTAGCAAGAT GATATCCGTG 
CCTGAAAGCG AGCTGCCGGA GAACAGGCGC CGCGGACCGC TTAGCCCTGA GGAGGCTCGA 
GAGCTGGCGA GAAAGAAGCG CGCCGAAGCC ATTCGTGCCC AAGGAGGCGC CGACGGTGAC 
CCTGACATGA ACGACCTGGC CAACGCCTTC ACCAGCACCT CCTTGACCGA CAATGCCGTG 
CCGGAACTCG AGGAGAAGCC CGTCGAAGCG GAGCATCTCA TCGGTTTTGC CCGTATCTAC 
TCGGGCACTC TATCCGTTGG CGACGAGATC TATGTGTTGC CCCCAAAGTT CTCCCCGGCT 
GATCCTCATG CCCAACCCGT CCCGAAGAAG GTGACGGTTA CCGCTCTCTA CATGCTCATG 
GGTCGCAATC TCGAAGCCCT TCCCTCCGTC CCAGCTGGTG TCGTGTTCGG TATTCGCGGT 
CTCGAAGGCA GCGGCCTTCT CAAGTCCGGA ACGCTCTGCA GCCAGCTCGA GGGCTCGGTC 
AACTTGGCTG GTATCGCCAA CCTGGCCGGT CGCCCCATTG TTCGTGTCGC TCTTGAGCCT 
GTCAACCCCT ATGACCTGGA CAAGATGATT CAGGGTCTTC ACCTCCTTGT CCAGAGCGAT 
CCGTGCGCTG AGTATGAGCA CTTCTCCAGT GGCGAGCACG TCCTCTCTAC TGCCGGCGAA 
TTGCATCTCG AACGCTGCCT GACGGATCTG CGCGAGCGGT TTGCGCGATG CGAGATCCAA 
GCCGGTGCGC CCATTGTGCC GTACCGGGAG ACGATCGTTC GTGCCGAAGA GATGCGCCCT 
CCAGTGAACA AGGAACTCGG CCGCGGTGTG GTTGTCGGCG TCACGAGCTC CAGGCAAGTG 
ACGGTGACCC TGCGCGTCAG GCCCCTCCCG GCCCCCGTAA CTGAATTCCT CCAGAAGAAC 
TCCGCCGGCA TCAAGCGGCT GTACACGGAC CGCAAGGCGG CTGGCGTCGA GGACGGCACC 
GAGTCACCCT CTGTAGAAGA GGCTACGTTG GAGAACGGCA CCCCGTCGGA CGTCAAGATT 
GAAGAAGACG AGGAGGTGTC CCAAGCCGCC ACCACCCTCA CGCCCGAGGA GCTCAAGAAG 
CAGCTCCAGG CCCAGCTCGA CGGCGTCAAA GACAAGGAGG CTTGGAAGGA CGTGATCGAC 
CGCATCGCCT CTTTTGGCCC GCGCAGAACG GGCGCCAACA TTCTCGTCGA CGCCACCAAG 
GACCAGCTCT TCAGCAAGGC CTTTTCGGCC GACAAGATCC GCAATCAGGC GCCCACGGGC 
GACGAGAAGC TGCACCCAGC CCACTTCAGC GATAAGATCA TGTACGGTTT CCAGCTGGCC 
ACGCAGCAAG GGCCGCTTTG CAACGAGCCC GTGCAGGGCA TCGCCGTCTT CGTGGAGGAT 
GTTCTGGTGG CGCAGACAGA GGACGACGAG GCCTCGTCGG CGCGTGACAG GATCGGCCGC 
TTGACGGGTG AGGTTATCAA GACGGTGCAG CAGTCGATTC ACAAGGGATT CTTGGACTGG 
TCGCCGCGCT TGATGTTGGC CATGTACTCG TGCGAGATTC AAGCGAGCAC CGAAGTCCTA 
GGCCGTGTCT ACGACGTGCT CACCCGCCGC CGCGGCCGCG TGCAGTCGGA AGCCATGAAC 
GAAGGCACGC CCTTTTTCAC TATCCAGGCC CTGCTGCCCG TGGCCGAGTC GTTCGGGTTC 
GCCGACGACA TGCGCAAGCG CACCTCGGGC GCCGCGCAGC CGCAGCTGAT CTTCACGGGT 
TACGAGATCC TGGACGAGGA TCCCTTCTGG GTGCCCTTCA CCGAAGACGA CTTGGAGGAC 
CTGGGCGAGT TTGGCGACAG GGAGAATGTG GCCAAGAGGT ATATGGATAG TGTGCGCAGG 
AGAAAGGGGT TGTTGGTGGA AGGGAGGAAT GTGGCTACTA ATGCGGAAAA GCAAAAGACT 
TTGAAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393908.1
CDS	288..3539
Translation	MPVVQPEKLAKLQQNADDVRNICILAHVDHGKTSLTDALLATNGIISPKLAGKIRYLDSRPDEQQRGITMESSAISLYFSMLRRSAPDATPEAKEYLINLIDSPGHIDFSSEVSTASRLCDGAVVLVDAVEGVCSQTVTVLRQAWTEKLKPLLVINKIDRLVTELKMTPGEAYIHLSKILEQVNAVLGSFFQGERMEEDLNWRDRMEERVAAAAAKEAQIAAGQPDSGELQFQEKDDEEIYFAPEKNNVIFGSAIDGWAFTVRQFAGMYEKKLGIKRGLLEKVLWGNFYMDPKTKKVLGPKHLKGRPLKPIFVQLVLEPIWAVYQATVGGDSGKGDPALLEKITKSLNLSVPPHILRSRDPKLLLTTVFASWLPLSTALLVSVVESLPSPKAAQADRLPDLLASVPGADHIDPKVKDAMVSFKKDPSEPMVAYVSKMISVPESELPENRRRGPLSPEEARELARKKRAEAIRAQGGADGDPDMNDLANAFTSTSLTDNAVPELEEKPVEAEHLIGFARIYSGTLSVGDEIYVLPPKFSPADPHAQPVPKKVTVTALYMLMGRNLEALPSVPAGVVFGIRGLEGSGLLKSGTLCSQLEGSVNLAGIANLAGRPIVRVALEPVNPYDLDKMIQGLHLLVQSDPCAEYEHFSSGEHVLSTAGELHLERCLTDLRERFARCEIQAGAPIVPYRETIVRAEEMRPPVNKELGRGVVVGVTSSRQVTVTLRVRPLPAPVTEFLQKNSAGIKRLYTDRKAAGVEDGTESPSVEEATLENGTPSDVKIEEDEEVSQAATTLTPEELKKQLQAQLDGVKDKEAWKDVIDRIASFGPRRTGANILVDATKDQLFSKAFSADKIRNQAPTGDEKLHPAHFSDKIMYGFQLATQQGPLCNEPVQGIAVFVEDVLVAQTEDDEASSARDRIGRLTGEVIKTVQQSIHKGFLDWSPRLMLAMYSCEIQASTEVLGRVYDVLTRRRGRVQSEAMNEGTPFFTIQALLPVAESFGFADDMRKRTSGAAQPQLIFTGYEILDEDPFWVPFTEDDLEDLGEFGDRENVAKRYMDSVRRRKGLLVEGRNVATNAEKQKTLKR

Target ORF information:

RefSeq Version	XM_011395606.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A elongation factor 2, variant 1 (NCU06278), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395606.1

ORF Insert Sequence:

ATGCCTGTTG TCCAGCCAGA AAAGCTGGCC AAGCTCCAAC AAAATGCCGA CGACGTGAGG 
AATATTTGTA TCTTGGCTCA CGTTGACCAT GGGAAAACAT CACTCACAGA TGCCCTTCTC 
GCCACCAATG GCATCATCTC ACCAAAACTC GCCGGAAAGA TTCGCTATCT CGATTCCCGG 
CCGGATGAAC AGCAAAGAGG TATCACCATG GAGTCTTCAG CCATTTCACT ATACTTCTCC 
ATGTTGAGGA GGTCGGCTCC TGACGCTACT CCCGAGGCCA AGGAGTACTT GATCAACCTG 
ATCGACTCCC CCGGACATAT CGATTTCAGC AGTGAAGTGT CCACTGCTTC TCGCCTTTGT 
GACGGTGCCG TTGTTCTGGT CGATGCTGTC GAAGGCGTCT GCAGTCAGAC AGTAACCGTG 
TTGCGCCAAG CATGGACCGA GAAGCTCAAG CCCCTTCTTG TTATCAACAA AATCGACCGC 
TTGGTTACCG AGTTGAAGAT GACCCCGGGC GAGGCTTATA TCCACCTCAG CAAAATTCTC 
GAGCAAGTCA ACGCAGTTCT TGGAAGCTTC TTCCAAGGTG AGCGCATGGA AGAAGATCTC 
AACTGGCGTG ACCGGATGGA GGAAAGAGTG GCTGCCGCCG CGGCCAAGGA GGCTCAAATA 
GCTGCCGGTC AACCTGACTC CGGCGAGCTT CAGTTCCAGG AAAAAGACGA CGAGGAGATT 
TACTTTGCGC CAGAGAAGAA CAACGTTATT TTTGGCAGTG CCATCGACGG CTGGGCTTTT 
ACTGTTCGCC AGTTTGCCGG CATGTACGAG AAGAAGCTCG GCATCAAGCG TGGCTTGCTG 
GAAAAGGTTC TCTGGGGCAA CTTCTACATG GATCCCAAGA CCAAAAAGGT ACTTGGGCCT 
AAGCATCTTA AGGGCAGACC TCTAAAGCCC ATCTTTGTCC AGTTGGTTCT GGAGCCAATT 
TGGGCTGTCT ACCAGGCCAC GGTTGGTGGT GACTCCGGTA AGGGTGATCC CGCTCTTTTG 
GAGAAGATCA CCAAGTCCTT GAACCTCAGT GTACCGCCCC ACATCCTCCG CTCTCGGGAT 
CCCAAACTAC TCCTTACCAC GGTGTTTGCT TCTTGGTTGC CTCTCTCTAC GGCTTTGCTC 
GTCTCAGTGG TGGAGTCATT GCCTTCGCCC AAAGCAGCTC AGGCGGACCG TCTCCCAGAC 
CTCCTGGCAA GCGTACCCGG CGCGGACCAC ATCGATCCCA AGGTCAAAGA TGCCATGGTC 
TCGTTCAAAA AAGATCCATC TGAGCCTATG GTTGCATATG TTAGCAAGAT GATATCCGTG 
CCTGAAAGCG AGCTGCCGGA GAACAGGCGC CGCGGACCGC TTAGCCCTGA GGAGGCTCGA 
GAGCTGGCGA GAAAGAAGCG CGCCGAAGCC ATTCGTGCCC AAGGAGGCGC CGACGGTGAC 
CCTGACATGA ACGACCTGGC CAACGCCTTC ACCAGCACCT CCTTGACCGA CAATGCCGTG 
CCGGAACTCG AGGAGAAGCC CGTCGAAGCG GAGCATCTCA TCGGTTTTGC CCGTATCTAC 
TCGGGCACTC TATCCGTTGG CGACGAGATC TATGTGTTGC CCCCAAAGTT CTCCCCGGCT 
GATCCTCATG CCCAACCCGT CCCGAAGAAG GTGACGGTTA CCGCTCTCTA CATGCTCATG 
GGTCGCAATC TCGAAGCCCT TCCCTCCGTC CCAGCTGGTG TCGTGTTCGG TATTCGCGGT 
CTCGAAGGCA GCGGCCTTCT CAAGTCCGGA ACGCTCTGCA GCCAGCTCGA GGGCTCGGTC 
AACTTGGCTG GTATCGCCAA CCTGGCCGGT CGCCCCATTG TTCGTGTCGC TCTTGAGCCT 
GTCAACCCCT ATGACCTGGA CAAGATGATT CAGGGTCTTC ACCTCCTTGT CCAGAGCGAT 
CCGTGCGCTG AGTATGAGCA CTTCTCCAGT GGCGAGCACG TCCTCTCTAC TGCCGGCGAA 
TTGCATCTCG AACGCTGCCT GACGGATCTG CGCGAGCGGT TTGCGCGATG CGAGATCCAA 
GCCGGTGCGC CCATTGTGCC GTACCGGGAG ACGATCGTTC GTGCCGAAGA GATGCGCCCT 
CCAGTGAACA AGGAACTCGG CCGCGGTGTG GTTGTCGGCG TCACGAGCTC CAGGCAAGTG 
ACGGTGACCC TGCGCGTCAG GCCCCTCCCG GCCCCCGTAA CTGAATTCCT CCAGAAGAAC 
TCCGCCGGCA TCAAGCGGCT GTACACGGAC CGCAAGGCGG CTGGCGTCGA GGACGGCACC 
GAGTCACCCT CTGTAGAAGA GGCTACGTTG GAGAACGGCA CCCCGTCGGA CGTCAAGATT 
GAAGAAGACG AGGAGGTGTC CCAAGCCGCC ACCACCCTCA CGCCCGAGGA GCTCAAGAAG 
CAGCTCCAGG CCCAGCTCGA CGGCGTCAAA GACAAGGAGG CTTGGAAGGA CGTGATCGAC 
CGCATCGCCT CTTTTGGCCC GCGCAGAACG GGCGCCAACA TTCTCGTCGA CGCCACCAAG 
GACCAGCTCT TCAGCAAGGC CTTTTCGGCC GACAAGATCC GCAATCAGGC GCCCACGGGC 
GACGAGAAGC TGCACCCAGC CCACTTCAGC GATAAGATCA TGTACGGTTT CCAGCTGGCC 
ACGCAGCAAG GGCCGCTTTG CAACGAGCCC GTGCAGGGCA TCGCCGTCTT CGTGGAGGAT 
GTTCTGGTGG CGCAGACAGA GGACGACGAG GCCTCGTCGG CGCGTGACAG GATCGGCCGC 
TTGACGGGTG AGGTTATCAA GACGGTGCAG CAGTCGATTC ACAAGGGATT CTTGGACTGG 
TCGCCGCGCT TGATGTTGGC CATGTACTCG TGCGAGATTC AAGCGAGCAC CGAAGTCCTA 
GGCCGTGTCT ACGACGTGCT CACCCGCCGC CGCGGCCGCG TGCAGTCGGA AGCCATGAAC 
GAAGGCACGC CCTTTTTCAC TATCCAGGCC CTGCTGCCCG TGGCCGAGTC GTTCGGGTTC 
GCCGACGACA TGCGCAAGCG CACCTCGGGC GCCGCGCAGC CGCAGCTGAT CTTCACGGGT 
TACGAGATCC TGGACGAGGA TCCCTTCTGG GTGCCCTTCA CCGAAGACGA CTTGGAGGAC 
CTGGGCGAGT TTGGCGACAG GGAGAATGTG GCCAAGAGGT ATATGGATAG TGTGCGCAGG 
AGAAAGGGGT TGTTGGTGGA AGGGAGGAAT GTGGCTACTA ATGCGGAAAA GCAAAAGACT 
TTGAAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU06278 ( XM_011395607.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393909.1 Neurospora crassa OR74A elongation factor 2, variant 2 (NCU06278), mRNA.

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CloneID	ONf12276
Clone ID Related Accession (Same CDS sequence)	XM_011395605.1 , XM_011395606.1 , XM_011395607.1
Accession Version	XM_011395607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	elongation factor 2, variant 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503).

ATGCCTGTTG TCCAGCCAGA AAAGCTGGCC AAGCTCCAAC AAAATGCCGA CGACGTGAGG 
AATATTTGTA TCTTGGCTCA CGTTGACCAT GGGAAAACAT CACTCACAGA TGCCCTTCTC 
GCCACCAATG GCATCATCTC ACCAAAACTC GCCGGAAAGA TTCGCTATCT CGATTCCCGG 
CCGGATGAAC AGCAAAGAGG TATCACCATG GAGTCTTCAG CCATTTCACT ATACTTCTCC 
ATGTTGAGGA GGTCGGCTCC TGACGCTACT CCCGAGGCCA AGGAGTACTT GATCAACCTG 
ATCGACTCCC CCGGACATAT CGATTTCAGC AGTGAAGTGT CCACTGCTTC TCGCCTTTGT 
GACGGTGCCG TTGTTCTGGT CGATGCTGTC GAAGGCGTCT GCAGTCAGAC AGTAACCGTG 
TTGCGCCAAG CATGGACCGA GAAGCTCAAG CCCCTTCTTG TTATCAACAA AATCGACCGC 
TTGGTTACCG AGTTGAAGAT GACCCCGGGC GAGGCTTATA TCCACCTCAG CAAAATTCTC 
GAGCAAGTCA ACGCAGTTCT TGGAAGCTTC TTCCAAGGTG AGCGCATGGA AGAAGATCTC 
AACTGGCGTG ACCGGATGGA GGAAAGAGTG GCTGCCGCCG CGGCCAAGGA GGCTCAAATA 
GCTGCCGGTC AACCTGACTC CGGCGAGCTT CAGTTCCAGG AAAAAGACGA CGAGGAGATT 
TACTTTGCGC CAGAGAAGAA CAACGTTATT TTTGGCAGTG CCATCGACGG CTGGGCTTTT 
ACTGTTCGCC AGTTTGCCGG CATGTACGAG AAGAAGCTCG GCATCAAGCG TGGCTTGCTG 
GAAAAGGTTC TCTGGGGCAA CTTCTACATG GATCCCAAGA CCAAAAAGGT ACTTGGGCCT 
AAGCATCTTA AGGGCAGACC TCTAAAGCCC ATCTTTGTCC AGTTGGTTCT GGAGCCAATT 
TGGGCTGTCT ACCAGGCCAC GGTTGGTGGT GACTCCGGTA AGGGTGATCC CGCTCTTTTG 
GAGAAGATCA CCAAGTCCTT GAACCTCAGT GTACCGCCCC ACATCCTCCG CTCTCGGGAT 
CCCAAACTAC TCCTTACCAC GGTGTTTGCT TCTTGGTTGC CTCTCTCTAC GGCTTTGCTC 
GTCTCAGTGG TGGAGTCATT GCCTTCGCCC AAAGCAGCTC AGGCGGACCG TCTCCCAGAC 
CTCCTGGCAA GCGTACCCGG CGCGGACCAC ATCGATCCCA AGGTCAAAGA TGCCATGGTC 
TCGTTCAAAA AAGATCCATC TGAGCCTATG GTTGCATATG TTAGCAAGAT GATATCCGTG 
CCTGAAAGCG AGCTGCCGGA GAACAGGCGC CGCGGACCGC TTAGCCCTGA GGAGGCTCGA 
GAGCTGGCGA GAAAGAAGCG CGCCGAAGCC ATTCGTGCCC AAGGAGGCGC CGACGGTGAC 
CCTGACATGA ACGACCTGGC CAACGCCTTC ACCAGCACCT CCTTGACCGA CAATGCCGTG 
CCGGAACTCG AGGAGAAGCC CGTCGAAGCG GAGCATCTCA TCGGTTTTGC CCGTATCTAC 
TCGGGCACTC TATCCGTTGG CGACGAGATC TATGTGTTGC CCCCAAAGTT CTCCCCGGCT 
GATCCTCATG CCCAACCCGT CCCGAAGAAG GTGACGGTTA CCGCTCTCTA CATGCTCATG 
GGTCGCAATC TCGAAGCCCT TCCCTCCGTC CCAGCTGGTG TCGTGTTCGG TATTCGCGGT 
CTCGAAGGCA GCGGCCTTCT CAAGTCCGGA ACGCTCTGCA GCCAGCTCGA GGGCTCGGTC 
AACTTGGCTG GTATCGCCAA CCTGGCCGGT CGCCCCATTG TTCGTGTCGC TCTTGAGCCT 
GTCAACCCCT ATGACCTGGA CAAGATGATT CAGGGTCTTC ACCTCCTTGT CCAGAGCGAT 
CCGTGCGCTG AGTATGAGCA CTTCTCCAGT GGCGAGCACG TCCTCTCTAC TGCCGGCGAA 
TTGCATCTCG AACGCTGCCT GACGGATCTG CGCGAGCGGT TTGCGCGATG CGAGATCCAA 
GCCGGTGCGC CCATTGTGCC GTACCGGGAG ACGATCGTTC GTGCCGAAGA GATGCGCCCT 
CCAGTGAACA AGGAACTCGG CCGCGGTGTG GTTGTCGGCG TCACGAGCTC CAGGCAAGTG 
ACGGTGACCC TGCGCGTCAG GCCCCTCCCG GCCCCCGTAA CTGAATTCCT CCAGAAGAAC 
TCCGCCGGCA TCAAGCGGCT GTACACGGAC CGCAAGGCGG CTGGCGTCGA GGACGGCACC 
GAGTCACCCT CTGTAGAAGA GGCTACGTTG GAGAACGGCA CCCCGTCGGA CGTCAAGATT 
GAAGAAGACG AGGAGGTGTC CCAAGCCGCC ACCACCCTCA CGCCCGAGGA GCTCAAGAAG 
CAGCTCCAGG CCCAGCTCGA CGGCGTCAAA GACAAGGAGG CTTGGAAGGA CGTGATCGAC 
CGCATCGCCT CTTTTGGCCC GCGCAGAACG GGCGCCAACA TTCTCGTCGA CGCCACCAAG 
GACCAGCTCT TCAGCAAGGC CTTTTCGGCC GACAAGATCC GCAATCAGGC GCCCACGGGC 
GACGAGAAGC TGCACCCAGC CCACTTCAGC GATAAGATCA TGTACGGTTT CCAGCTGGCC 
ACGCAGCAAG GGCCGCTTTG CAACGAGCCC GTGCAGGGCA TCGCCGTCTT CGTGGAGGAT 
GTTCTGGTGG CGCAGACAGA GGACGACGAG GCCTCGTCGG CGCGTGACAG GATCGGCCGC 
TTGACGGGTG AGGTTATCAA GACGGTGCAG CAGTCGATTC ACAAGGGATT CTTGGACTGG 
TCGCCGCGCT TGATGTTGGC CATGTACTCG TGCGAGATTC AAGCGAGCAC CGAAGTCCTA 
GGCCGTGTCT ACGACGTGCT CACCCGCCGC CGCGGCCGCG TGCAGTCGGA AGCCATGAAC 
GAAGGCACGC CCTTTTTCAC TATCCAGGCC CTGCTGCCCG TGGCCGAGTC GTTCGGGTTC 
GCCGACGACA TGCGCAAGCG CACCTCGGGC GCCGCGCAGC CGCAGCTGAT CTTCACGGGT 
TACGAGATCC TGGACGAGGA TCCCTTCTGG GTGCCCTTCA CCGAAGACGA CTTGGAGGAC 
CTGGGCGAGT TTGGCGACAG GGAGAATGTG GCCAAGAGGT ATATGGATAG TGTGCGCAGG 
AGAAAGGGGT TGTTGGTGGA AGGGAGGAAT GTGGCTACTA ATGCGGAAAA GCAAAAGACT 
TTGAAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393909.1
CDS	257..3508
Translation	MPVVQPEKLAKLQQNADDVRNICILAHVDHGKTSLTDALLATNGIISPKLAGKIRYLDSRPDEQQRGITMESSAISLYFSMLRRSAPDATPEAKEYLINLIDSPGHIDFSSEVSTASRLCDGAVVLVDAVEGVCSQTVTVLRQAWTEKLKPLLVINKIDRLVTELKMTPGEAYIHLSKILEQVNAVLGSFFQGERMEEDLNWRDRMEERVAAAAAKEAQIAAGQPDSGELQFQEKDDEEIYFAPEKNNVIFGSAIDGWAFTVRQFAGMYEKKLGIKRGLLEKVLWGNFYMDPKTKKVLGPKHLKGRPLKPIFVQLVLEPIWAVYQATVGGDSGKGDPALLEKITKSLNLSVPPHILRSRDPKLLLTTVFASWLPLSTALLVSVVESLPSPKAAQADRLPDLLASVPGADHIDPKVKDAMVSFKKDPSEPMVAYVSKMISVPESELPENRRRGPLSPEEARELARKKRAEAIRAQGGADGDPDMNDLANAFTSTSLTDNAVPELEEKPVEAEHLIGFARIYSGTLSVGDEIYVLPPKFSPADPHAQPVPKKVTVTALYMLMGRNLEALPSVPAGVVFGIRGLEGSGLLKSGTLCSQLEGSVNLAGIANLAGRPIVRVALEPVNPYDLDKMIQGLHLLVQSDPCAEYEHFSSGEHVLSTAGELHLERCLTDLRERFARCEIQAGAPIVPYRETIVRAEEMRPPVNKELGRGVVVGVTSSRQVTVTLRVRPLPAPVTEFLQKNSAGIKRLYTDRKAAGVEDGTESPSVEEATLENGTPSDVKIEEDEEVSQAATTLTPEELKKQLQAQLDGVKDKEAWKDVIDRIASFGPRRTGANILVDATKDQLFSKAFSADKIRNQAPTGDEKLHPAHFSDKIMYGFQLATQQGPLCNEPVQGIAVFVEDVLVAQTEDDEASSARDRIGRLTGEVIKTVQQSIHKGFLDWSPRLMLAMYSCEIQASTEVLGRVYDVLTRRRGRVQSEAMNEGTPFFTIQALLPVAESFGFADDMRKRTSGAAQPQLIFTGYEILDEDPFWVPFTEDDLEDLGEFGDRENVAKRYMDSVRRRKGLLVEGRNVATNAEKQKTLKR

Target ORF information:

RefSeq Version	XM_011395607.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A elongation factor 2, variant 2 (NCU06278), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395607.1

ORF Insert Sequence:

ATGCCTGTTG TCCAGCCAGA AAAGCTGGCC AAGCTCCAAC AAAATGCCGA CGACGTGAGG 
AATATTTGTA TCTTGGCTCA CGTTGACCAT GGGAAAACAT CACTCACAGA TGCCCTTCTC 
GCCACCAATG GCATCATCTC ACCAAAACTC GCCGGAAAGA TTCGCTATCT CGATTCCCGG 
CCGGATGAAC AGCAAAGAGG TATCACCATG GAGTCTTCAG CCATTTCACT ATACTTCTCC 
ATGTTGAGGA GGTCGGCTCC TGACGCTACT CCCGAGGCCA AGGAGTACTT GATCAACCTG 
ATCGACTCCC CCGGACATAT CGATTTCAGC AGTGAAGTGT CCACTGCTTC TCGCCTTTGT 
GACGGTGCCG TTGTTCTGGT CGATGCTGTC GAAGGCGTCT GCAGTCAGAC AGTAACCGTG 
TTGCGCCAAG CATGGACCGA GAAGCTCAAG CCCCTTCTTG TTATCAACAA AATCGACCGC 
TTGGTTACCG AGTTGAAGAT GACCCCGGGC GAGGCTTATA TCCACCTCAG CAAAATTCTC 
GAGCAAGTCA ACGCAGTTCT TGGAAGCTTC TTCCAAGGTG AGCGCATGGA AGAAGATCTC 
AACTGGCGTG ACCGGATGGA GGAAAGAGTG GCTGCCGCCG CGGCCAAGGA GGCTCAAATA 
GCTGCCGGTC AACCTGACTC CGGCGAGCTT CAGTTCCAGG AAAAAGACGA CGAGGAGATT 
TACTTTGCGC CAGAGAAGAA CAACGTTATT TTTGGCAGTG CCATCGACGG CTGGGCTTTT 
ACTGTTCGCC AGTTTGCCGG CATGTACGAG AAGAAGCTCG GCATCAAGCG TGGCTTGCTG 
GAAAAGGTTC TCTGGGGCAA CTTCTACATG GATCCCAAGA CCAAAAAGGT ACTTGGGCCT 
AAGCATCTTA AGGGCAGACC TCTAAAGCCC ATCTTTGTCC AGTTGGTTCT GGAGCCAATT 
TGGGCTGTCT ACCAGGCCAC GGTTGGTGGT GACTCCGGTA AGGGTGATCC CGCTCTTTTG 
GAGAAGATCA CCAAGTCCTT GAACCTCAGT GTACCGCCCC ACATCCTCCG CTCTCGGGAT 
CCCAAACTAC TCCTTACCAC GGTGTTTGCT TCTTGGTTGC CTCTCTCTAC GGCTTTGCTC 
GTCTCAGTGG TGGAGTCATT GCCTTCGCCC AAAGCAGCTC AGGCGGACCG TCTCCCAGAC 
CTCCTGGCAA GCGTACCCGG CGCGGACCAC ATCGATCCCA AGGTCAAAGA TGCCATGGTC 
TCGTTCAAAA AAGATCCATC TGAGCCTATG GTTGCATATG TTAGCAAGAT GATATCCGTG 
CCTGAAAGCG AGCTGCCGGA GAACAGGCGC CGCGGACCGC TTAGCCCTGA GGAGGCTCGA 
GAGCTGGCGA GAAAGAAGCG CGCCGAAGCC ATTCGTGCCC AAGGAGGCGC CGACGGTGAC 
CCTGACATGA ACGACCTGGC CAACGCCTTC ACCAGCACCT CCTTGACCGA CAATGCCGTG 
CCGGAACTCG AGGAGAAGCC CGTCGAAGCG GAGCATCTCA TCGGTTTTGC CCGTATCTAC 
TCGGGCACTC TATCCGTTGG CGACGAGATC TATGTGTTGC CCCCAAAGTT CTCCCCGGCT 
GATCCTCATG CCCAACCCGT CCCGAAGAAG GTGACGGTTA CCGCTCTCTA CATGCTCATG 
GGTCGCAATC TCGAAGCCCT TCCCTCCGTC CCAGCTGGTG TCGTGTTCGG TATTCGCGGT 
CTCGAAGGCA GCGGCCTTCT CAAGTCCGGA ACGCTCTGCA GCCAGCTCGA GGGCTCGGTC 
AACTTGGCTG GTATCGCCAA CCTGGCCGGT CGCCCCATTG TTCGTGTCGC TCTTGAGCCT 
GTCAACCCCT ATGACCTGGA CAAGATGATT CAGGGTCTTC ACCTCCTTGT CCAGAGCGAT 
CCGTGCGCTG AGTATGAGCA CTTCTCCAGT GGCGAGCACG TCCTCTCTAC TGCCGGCGAA 
TTGCATCTCG AACGCTGCCT GACGGATCTG CGCGAGCGGT TTGCGCGATG CGAGATCCAA 
GCCGGTGCGC CCATTGTGCC GTACCGGGAG ACGATCGTTC GTGCCGAAGA GATGCGCCCT 
CCAGTGAACA AGGAACTCGG CCGCGGTGTG GTTGTCGGCG TCACGAGCTC CAGGCAAGTG 
ACGGTGACCC TGCGCGTCAG GCCCCTCCCG GCCCCCGTAA CTGAATTCCT CCAGAAGAAC 
TCCGCCGGCA TCAAGCGGCT GTACACGGAC CGCAAGGCGG CTGGCGTCGA GGACGGCACC 
GAGTCACCCT CTGTAGAAGA GGCTACGTTG GAGAACGGCA CCCCGTCGGA CGTCAAGATT 
GAAGAAGACG AGGAGGTGTC CCAAGCCGCC ACCACCCTCA CGCCCGAGGA GCTCAAGAAG 
CAGCTCCAGG CCCAGCTCGA CGGCGTCAAA GACAAGGAGG CTTGGAAGGA CGTGATCGAC 
CGCATCGCCT CTTTTGGCCC GCGCAGAACG GGCGCCAACA TTCTCGTCGA CGCCACCAAG 
GACCAGCTCT TCAGCAAGGC CTTTTCGGCC GACAAGATCC GCAATCAGGC GCCCACGGGC 
GACGAGAAGC TGCACCCAGC CCACTTCAGC GATAAGATCA TGTACGGTTT CCAGCTGGCC 
ACGCAGCAAG GGCCGCTTTG CAACGAGCCC GTGCAGGGCA TCGCCGTCTT CGTGGAGGAT 
GTTCTGGTGG CGCAGACAGA GGACGACGAG GCCTCGTCGG CGCGTGACAG GATCGGCCGC 
TTGACGGGTG AGGTTATCAA GACGGTGCAG CAGTCGATTC ACAAGGGATT CTTGGACTGG 
TCGCCGCGCT TGATGTTGGC CATGTACTCG TGCGAGATTC AAGCGAGCAC CGAAGTCCTA 
GGCCGTGTCT ACGACGTGCT CACCCGCCGC CGCGGCCGCG TGCAGTCGGA AGCCATGAAC 
GAAGGCACGC CCTTTTTCAC TATCCAGGCC CTGCTGCCCG TGGCCGAGTC GTTCGGGTTC 
GCCGACGACA TGCGCAAGCG CACCTCGGGC GCCGCGCAGC CGCAGCTGAT CTTCACGGGT 
TACGAGATCC TGGACGAGGA TCCCTTCTGG GTGCCCTTCA CCGAAGACGA CTTGGAGGAC 
CTGGGCGAGT TTGGCGACAG GGAGAATGTG GCCAAGAGGT ATATGGATAG TGTGCGCAGG 
AGAAAGGGGT TGTTGGTGGA AGGGAGGAAT GTGGCTACTA ATGCGGAAAA GCAAAAGACT 
TTGAAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU06278 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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