C4A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	C4A
Entrez Gene ID	720
Full Name	complement C4A (Rodgers blood group)
Synonyms	C4,C4A2,C4A3,C4A4,C4A6,C4AD,C4S,CO4,CPAMD2,RG
General protein information	Preferred Names complement C4A (Rodgers blood group) Names complement C4-A C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 C4A anaphylatoxin MHC class III region complement Rodgers form of C4 acidic C4 acidic complement C4 complement component 4A (Rodgers blood group)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.33
Summary	This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014].
Disorder MIM:	120810
Disorder Html:	C4a deficiency, 614380 (3); [Blood group, Rodgers], 614374 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_007293.2	NP_009224.2	complement C4-A isoform 1 preproprotein
XM_017030289.1	XP_016885778.1	complement C4-A isoform X1
NM_007293.3	NP_009224.2	complement C4-A isoform 1 preproprotein
NM_001252204.1	NP_001239133.1	complement C4-A isoform 2 preproprotein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	C4a	NP_035543.2
Danio rerio (zebrafish)	si:dkey-8k3.2	XP_001334640.1
Mus musculus (house mouse)	C4b	NP_033910.2
Rattus norvegicus (Norway rat)	C4b	NP_001002805.1
Homo sapiens (human)	C4A	NP_009224.2
Pan troglodytes (chimpanzee)	C4A	XP_003311220.1
Canis lupus familiaris (dog)	C4A	XP_005627805.1
Bos taurus (cattle)	C4A	NP_001159957.1
Gallus gallus (chicken)	C4	NP_001070701.1
Homo sapiens (human)	C4B	NP_001002029.3
Xenopus tropicalis (tropical clawed frog)	c4a	NP_001107156.1
Rattus norvegicus (Norway rat)	C4a	NP_113692.2

Gene Ontology
Bibliography

Related articles in PubMed

Preservation of antimicrobial properties of complement peptide C3a, from invertebrates to humans.
Pasupuleti M, Walse B, Nordahl EA, M?rgelin M, Malmsten M, Schmidtchen A
The Journal of biological chemistry282(4)2520-8(2007 Jan)

The structural basis of the multiple forms of human complement component C4.
Belt KT, Carroll MC, Porter RR
Cell36(4)907-14(1984 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

for the first time, a complete overview of C4 in SLE from genetic variation to binding capacity using a novel test. As this test detects crossing over of Rodgers and Chido antigens, it will allow for more accurate measurement of C4 in future studies.
Title: Comprehensive approach to study complement C4 in systemic lupus erythematosus: Gene polymorphisms, protein levels and functional activity.

This study shows that the C4c/C4 ratio seems to be a better diagnostic measure than total antigenic C4 alone. Our findings underline that screening with total antigenic C4 implies a risk of overlooking C1-INH-HAE patients.
Title: Complement factor C4 activation in patients with hereditary angioedema.

An elevated number of C4 genes was observed in Alzheimer's disease (AD) patients as compared with healthy controls. The presence of high C4A and C4B copy numbers in AD patients could explain the increased C4 protein expression observed in AD patients, thus highlighting a possible role for C4A and C4B copy number variations in the risk of developing AD.
Title: Complement C4A and C4B Gene Copy Number Study in Alzheimer's Disease Patients.

Coronary atherosclerosis is distinguished by serum C4 complement up-regulation and ceruloplasmin down-regulation.
Title: Proteomic Study of Blood Serum in Coronary Atherosclerosis.

The study re-evaluates low-resolution crystal structures of C4 via interactive molecular-dynamics flexible fitting. In terms of biology, the results provide a better structural framework for understanding the pivotal function of the C4 protein within the complement system.
Title: Re-evaluation of low-resolution crystal structures via interactive molecular-dynamics flexible fitting (iMDFF): a case study in complement C4.

The following C4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the C4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11432	NM_007293.3 Latest version!	Homo sapiens complement C4A (Rodgers blood group) (C4A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu99646	XM_017030289.1 Latest version!	Homo sapiens complement component 4A (Rodgers blood group) (C4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu10491	NM_001252204.1 Latest version!	Homo sapiens complement C4A (Rodgers blood group) (C4A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu11432	NM_007293.2	Homo sapiens complement C4A (Rodgers blood group) (C4A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

C4A ( NM_007293.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_009224.2 Homo sapiens complement C4A (Rodgers blood group) (C4A), transcript variant 1, mRNA.

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CloneID	OHu11432
Clone ID Related Accession (Same CDS sequence)	NM_007293.2 , NM_007293.3
Accession Version	NM_007293.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	complement C4-A isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA304049.1, K02403.1, AL645922.12 and BC146673.1. On or before Mar 22, 2015 this sequence version replaced XM_011548125.1, XM_006725947.1, XM_003960046.2, XM_003403789.1, NM_007293.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record represents the C4A*001.1.1 allele. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.237372.1, SRR1803615.205721.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 17132627 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGCTGC TCTGGGGGCT GATCTGGGCA TCCAGCTTCT TCACCTTATC TCTGCAGAAG 
CCCAGGTTGC TCTTGTTCTC TCCTTCTGTG GTTCATCTGG GGGTCCCCCT ATCGGTGGGG 
GTGCAGCTCC AGGATGTGCC CCGAGGACAG GTAGTGAAAG GATCAGTGTT CCTGAGAAAC 
CCATCTCGTA ATAATGTCCC CTGCTCCCCA AAGGTGGACT TCACCCTTAG CTCAGAAAGA 
GACTTCGCAC TCCTCAGTCT CCAGGTGCCC TTGAAAGATG CGAAGAGCTG TGGCCTCCAT 
CAACTCCTCA GAGGCCCTGA GGTCCAGCTG GTGGCCCATT CGCCATGGCT AAAGGACTCT 
CTGTCCAGAA CGACAAACAT CCAGGGTATC AACCTGCTCT TCTCCTCTCG CCGGGGGCAC 
CTCTTTTTGC AGACGGACCA GCCCATTTAC AACCCTGGCC AGCGGGTTCG GTACCGGGTC 
TTTGCTCTGG ATCAGAAGAT GCGCCCGAGC ACTGACACCA TCACAGTCAT GGTGGAGAAC 
TCTCACGGCC TCCGCGTGCG GAAGAAGGAG GTGTACATGC CCTCGTCCAT CTTCCAGGAT 
GACTTTGTGA TCCCAGACAT CTCAGAGCCA GGGACCTGGA AGATCTCAGC CCGATTCTCA 
GATGGCCTGG AATCCAACAG CAGCACCCAG TTTGAGGTGA AGAAATATGT CCTTCCCAAC 
TTTGAGGTGA AGATCACCCC TGGAAAGCCC TACATCCTGA CGGTGCCAGG CCATCTTGAT 
GAAATGCAGT TAGACATCCA GGCCAGGTAC ATCTATGGGA AGCCAGTGCA GGGGGTGGCA 
TATGTGCGCT TTGGGCTCCT AGATGAGGAT GGTAAGAAGA CTTTCTTTCG GGGGCTGGAG 
AGTCAGACCA AGCTGGTGAA TGGACAGAGC CACATTTCCC TCTCAAAGGC AGAGTTCCAG 
GACGCCCTGG AGAAGCTGAA TATGGGCATT ACTGACCTCC AGGGGCTGCG CCTCTACGTT 
GCTGCAGCCA TCATTGAGTC TCCAGGTGGG GAGATGGAGG AGGCAGAGCT CACATCCTGG 
TATTTTGTGT CATCTCCCTT CTCCTTGGAT CTTAGCAAGA CCAAGCGACA CCTTGTGCCT 
GGGGCCCCCT TCCTGCTGCA GGCCTTGGTC CGTGAGATGT CAGGCTCCCC AGCTTCTGGC 
ATTCCTGTCA AAGTTTCTGC CACGGTGTCT TCTCCTGGGT CTGTTCCTGA AGTCCAGGAC 
ATTCAGCAAA ACACAGACGG GAGCGGCCAA GTCAGCATTC CAATAATTAT CCCTCAGACC 
ATCTCAGAGC TGCAGCTCTC AGTATCTGCA GGCTCCCCAC ATCCAGCGAT AGCCAGGCTC 
ACTGTGGCAG CCCCACCTTC AGGAGGCCCC GGGTTTCTGT CTATTGAGCG GCCGGATTCT 
CGACCTCCTC GTGTTGGGGA CACTCTGAAC CTGAACTTGC GAGCCGTGGG CAGTGGGGCC 
ACCTTTTCTC ATTACTACTA CATGATCCTA TCCCGAGGGC AGATCGTGTT CATGAATCGA 
GAGCCCAAGA GGACCCTGAC CTCGGTCTCG GTGTTTGTGG ACCATCACCT GGCACCCTCC 
TTCTACTTTG TGGCCTTCTA CTACCATGGA GACCACCCAG TGGCCAACTC CCTGCGAGTG 
GATGTCCAGG CTGGGGCCTG CGAGGGCAAG CTGGAGCTCA GCGTGGACGG TGCCAAGCAG 
TACCGGAACG GGGAGTCCGT GAAGCTCCAC TTAGAAACCG ACTCCCTAGC CCTGGTGGCG 
CTGGGAGCCT TGGACACAGC TCTGTATGCT GCAGGCAGCA AGTCCCACAA GCCCCTCAAC 
ATGGGCAAGG TCTTTGAAGC TATGAACAGC TATGACCTCG GCTGTGGTCC TGGGGGTGGG 
GACAGTGCCC TTCAGGTGTT CCAGGCAGCG GGCCTGGCCT TTTCTGATGG AGACCAGTGG 
ACCTTATCCA GAAAGAGACT AAGCTGTCCC AAGGAGAAGA CAACCCGGAA AAAGAGAAAC 
GTGAACTTCC AAAAGGCGAT TAATGAGAAA TTGGGTCAGT ATGCTTCCCC GACAGCCAAG 
CGCTGCTGCC AGGATGGGGT GACACGTCTG CCCATGATGC GTTCCTGCGA GCAGCGGGCA 
GCCCGCGTGC AGCAGCCGGA CTGCCGGGAG CCCTTCCTGT CCTGCTGCCA ATTTGCTGAG 
AGTCTGCGCA AGAAGAGCAG GGACAAGGGC CAGGCGGGCC TCCAACGAGC CCTGGAGATC 
CTGCAGGAGG AGGACCTGAT TGATGAGGAT GACATTCCCG TGCGCAGCTT CTTCCCAGAG 
AACTGGCTCT GGAGAGTGGA AACAGTGGAC CGCTTTCAAA TATTGACACT GTGGCTCCCC 
GACTCTCTGA CCACGTGGGA GATCCATGGC CTGAGCCTGT CCAAAACCAA AGGCCTATGT 
GTGGCCACCC CAGTCCAGCT CCGGGTGTTC CGCGAGTTCC ACCTGCACCT CCGCCTGCCC 
ATGTCTGTCC GCCGCTTTGA GCAGCTGGAG CTGCGGCCTG TCCTCTATAA CTACCTGGAT 
AAAAACCTGA CTGTGAGCGT CCACGTGTCC CCAGTGGAGG GGCTGTGCCT GGCTGGGGGC 
GGAGGGCTGG CCCAGCAGGT GCTGGTGCCT GCGGGCTCTG CCCGGCCTGT TGCCTTCTCT 
GTGGTGCCCA CGGCAGCCGC CGCTGTGTCT CTGAAGGTGG TGGCTCGAGG GTCCTTCGAA 
TTCCCTGTGG GAGATGCGGT GTCCAAGGTT CTGCAGATTG AGAAGGAAGG GGCCATCCAT 
AGAGAGGAGC TGGTCTATGA ACTCAACCCC TTGGACCACC GAGGCCGGAC CTTGGAAATA 
CCTGGCAACT CTGATCCCAA TATGATCCCT GATGGGGACT TTAACAGCTA CGTCAGGGTT 
ACAGCCTCAG ATCCATTGGA CACTTTAGGC TCTGAGGGGG CCTTGTCACC AGGAGGCGTG 
GCCTCCCTCT TGAGGCTTCC TCGAGGCTGT GGGGAGCAAA CCATGATCTA CTTGGCTCCG 
ACACTGGCTG CTTCCCGCTA CCTGGACAAG ACAGAGCAGT GGAGCACACT GCCTCCCGAG 
ACCAAGGACC ACGCCGTGGA TCTGATCCAG AAAGGCTACA TGCGGATCCA GCAGTTTCGG 
AAGGCGGATG GTTCCTATGC GGCTTGGTTG TCACGGGACA GCAGCACCTG GCTCACAGCC 
TTTGTGTTGA AGGTCCTGAG TTTGGCCCAG GAGCAGGTAG GAGGCTCGCC TGAGAAACTG 
CAGGAGACAT CTAACTGGCT TCTGTCCCAG CAGCAGGCTG ACGGCTCGTT CCAGGACCCC 
TGTCCAGTGT TAGACAGGAG CATGCAGGGG GGTTTGGTGG GCAATGATGA GACTGTGGCA 
CTCACAGCCT TTGTGACCAT CGCCCTTCAT CATGGGCTGG CCGTCTTCCA GGATGAGGGT 
GCAGAGCCAT TGAAGCAGAG AGTGGAAGCC TCCATCTCAA AGGCAAACTC ATTTTTGGGG 
GAGAAAGCAA GTGCTGGGCT CCTGGGTGCC CACGCAGCTG CCATCACGGC CTATGCCCTG 
ACACTGACCA AGGCGCCTGT GGACCTGCTC GGTGTTGCCC ACAACAACCT CATGGCAATG 
GCCCAGGAGA CTGGAGATAA CCTGTACTGG GGCTCAGTCA CTGGTTCTCA GAGCAATGCC 
GTGTCGCCCA CCCCGGCTCC TCGCAACCCA TCCGACCCCA TGCCCCAGGC CCCAGCCCTG 
TGGATTGAAA CCACAGCCTA CGCCCTGCTG CACCTCCTGC TTCACGAGGG CAAAGCAGAG 
ATGGCAGACC AGGCTTCGGC CTGGCTCACC CGTCAGGGCA GCTTCCAAGG GGGATTCCGC 
AGTACCCAAG ACACGGTGAT TGCCCTGGAT GCCCTGTCTG CCTACTGGAT TGCCTCCCAC 
ACCACTGAGG AGAGGGGTCT CAATGTGACT CTCAGCTCCA CAGGCCGGAA TGGGTTCAAG 
TCCCACGCGC TGCAGCTGAA CAACCGCCAG ATTCGCGGCC TGGAGGAGGA GCTGCAGTTT 
TCCTTGGGCA GCAAGATCAA TGTGAAGGTG GGAGGAAACA GCAAAGGAAC CCTGAAGGTC 
CTTCGTACCT ACAATGTCCT GGACATGAAG AACACGACCT GCCAGGACCT ACAGATAGAA 
GTGACAGTCA AAGGCCACGT CGAGTACACG ATGGAAGCAA ACGAGGACTA TGAGGACTAT 
GAGTACGATG AGCTTCCAGC CAAGGATGAC CCAGATGCCC CTCTGCAGCC CGTGACACCC 
CTGCAGCTGT TTGAGGGTCG GAGGAACCGC CGCAGGAGGG AGGCGCCCAA GGTGGTGGAG 
GAGCAGGAGT CCAGGGTGCA CTACACCGTG TGCATCTGGC GGAACGGCAA GGTGGGGCTG 
TCTGGCATGG CCATCGCGGA CGTCACCCTC CTGAGTGGAT TCCACGCCCT GCGTGCTGAC 
CTGGAGAAGC TGACCTCCCT CTCTGACCGT TACGTGAGTC ACTTTGAGAC CGAGGGGCCC 
CACGTCCTGC TGTATTTTGA CTCGGTCCCC ACCTCCCGGG AGTGCGTGGG CTTTGAGGCT 
GTGCAGGAAG TGCCGGTGGG GCTGGTGCAG CCGGCCAGCG CAACCCTGTA CGACTACTAC 
AACCCCGAGC GCAGATGTTC TGTGTTTTAC GGGGCACCAA GTAAGAGCAG ACTCTTGGCC 
ACCTTGTGTT CTGCTGAAGT CTGCCAGTGT GCTGAGGGGA AGTGCCCTCG CCAGCGTCGC 
GCCCTGGAGC GGGGTCTGCA GGACGAGGAT GGCTACAGGA TGAAGTTTGC CTGCTACTAC 
CCCCGTGTGG AGTACGGCTT CCAGGTTAAG GTTCTCCGAG AAGACAGCAG AGCTGCTTTC 
CGCCTCTTTG AGACCAAGAT CACCCAAGTC CTGCACTTCA CCAAGGATGT CAAGGCCGCT 
GCTAATCAGA TGCGCAACTT CCTGGTTCGA GCCTCCTGCC GCCTTCGCTT GGAACCTGGG 
AAAGAATATT TGATCATGGG TCTGGATGGG GCCACCTATG ACCTCGAGGG ACACCCCCAG 
TACCTGCTGG ACTCGAATAG CTGGATCGAG GAGATGCCCT CTGAACGCCT GTGCCGGAGC 
ACCCGCCAGC GGGCAGCCTG TGCCCAGCTC AACGACTTCC TCCAGGAGTA TGGCACTCAG 
GGGTGCCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009224.2
CDS	52..5286
Translation	MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAGLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKANSFLGEKASAGLLGAHAAAITAYALTLTKAPVDLLGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQASAWLTRQGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQIRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREDSRAAFRLFETKITQVLHFTKDVKAAANQMRNFLVRASCRLRLEPGKEYLIMGLDGATYDLEGHPQYLLDSNSWIEEMPSERLCRSTRQRAACAQLNDFLQEYGTQGCQV

Target ORF information:

RefSeq Version	NM_007293.2
Organism	Homo sapiens(human)
Definition	Homo sapiens complement C4A (Rodgers blood group) (C4A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007293.2

ORF Insert Sequence:

ATGAGGCTGC TCTGGGGGCT GATCTGGGCA TCCAGCTTCT TCACCTTATC TCTGCAGAAG 
CCCAGGTTGC TCTTGTTCTC TCCTTCTGTG GTTCATCTGG GGGTCCCCCT ATCGGTGGGG 
GTGCAGCTCC AGGATGTGCC CCGAGGACAG GTAGTGAAAG GATCAGTGTT CCTGAGAAAC 
CCATCTCGTA ATAATGTCCC CTGCTCCCCA AAGGTGGACT TCACCCTTAG CTCAGAAAGA 
GACTTCGCAC TCCTCAGTCT CCAGGTGCCC TTGAAAGATG CGAAGAGCTG TGGCCTCCAT 
CAACTCCTCA GAGGCCCTGA GGTCCAGCTG GTGGCCCATT CGCCATGGCT AAAGGACTCT 
CTGTCCAGAA CGACAAACAT CCAGGGTATC AACCTGCTCT TCTCCTCTCG CCGGGGGCAC 
CTCTTTTTGC AGACGGACCA GCCCATTTAC AACCCTGGCC AGCGGGTTCG GTACCGGGTC 
TTTGCTCTGG ATCAGAAGAT GCGCCCGAGC ACTGACACCA TCACAGTCAT GGTGGAGAAC 
TCTCACGGCC TCCGCGTGCG GAAGAAGGAG GTGTACATGC CCTCGTCCAT CTTCCAGGAT 
GACTTTGTGA TCCCAGACAT CTCAGAGCCA GGGACCTGGA AGATCTCAGC CCGATTCTCA 
GATGGCCTGG AATCCAACAG CAGCACCCAG TTTGAGGTGA AGAAATATGT CCTTCCCAAC 
TTTGAGGTGA AGATCACCCC TGGAAAGCCC TACATCCTGA CGGTGCCAGG CCATCTTGAT 
GAAATGCAGT TAGACATCCA GGCCAGGTAC ATCTATGGGA AGCCAGTGCA GGGGGTGGCA 
TATGTGCGCT TTGGGCTCCT AGATGAGGAT GGTAAGAAGA CTTTCTTTCG GGGGCTGGAG 
AGTCAGACCA AGCTGGTGAA TGGACAGAGC CACATTTCCC TCTCAAAGGC AGAGTTCCAG 
GACGCCCTGG AGAAGCTGAA TATGGGCATT ACTGACCTCC AGGGGCTGCG CCTCTACGTT 
GCTGCAGCCA TCATTGAGTC TCCAGGTGGG GAGATGGAGG AGGCAGAGCT CACATCCTGG 
TATTTTGTGT CATCTCCCTT CTCCTTGGAT CTTAGCAAGA CCAAGCGACA CCTTGTGCCT 
GGGGCCCCCT TCCTGCTGCA GGCCTTGGTC CGTGAGATGT CAGGCTCCCC AGCTTCTGGC 
ATTCCTGTCA AAGTTTCTGC CACGGTGTCT TCTCCTGGGT CTGTTCCTGA AGTCCAGGAC 
ATTCAGCAAA ACACAGACGG GAGCGGCCAA GTCAGCATTC CAATAATTAT CCCTCAGACC 
ATCTCAGAGC TGCAGCTCTC AGTATCTGCA GGCTCCCCAC ATCCAGCGAT AGCCAGGCTC 
ACTGTGGCAG CCCCACCTTC AGGAGGCCCC GGGTTTCTGT CTATTGAGCG GCCGGATTCT 
CGACCTCCTC GTGTTGGGGA CACTCTGAAC CTGAACTTGC GAGCCGTGGG CAGTGGGGCC 
ACCTTTTCTC ATTACTACTA CATGATCCTA TCCCGAGGGC AGATCGTGTT CATGAATCGA 
GAGCCCAAGA GGACCCTGAC CTCGGTCTCG GTGTTTGTGG ACCATCACCT GGCACCCTCC 
TTCTACTTTG TGGCCTTCTA CTACCATGGA GACCACCCAG TGGCCAACTC CCTGCGAGTG 
GATGTCCAGG CTGGGGCCTG CGAGGGCAAG CTGGAGCTCA GCGTGGACGG TGCCAAGCAG 
TACCGGAACG GGGAGTCCGT GAAGCTCCAC TTAGAAACCG ACTCCCTAGC CCTGGTGGCG 
CTGGGAGCCT TGGACACAGC TCTGTATGCT GCAGGCAGCA AGTCCCACAA GCCCCTCAAC 
ATGGGCAAGG TCTTTGAAGC TATGAACAGC TATGACCTCG GCTGTGGTCC TGGGGGTGGG 
GACAGTGCCC TTCAGGTGTT CCAGGCAGCG GGCCTGGCCT TTTCTGATGG AGACCAGTGG 
ACCTTATCCA GAAAGAGACT AAGCTGTCCC AAGGAGAAGA CAACCCGGAA AAAGAGAAAC 
GTGAACTTCC AAAAGGCGAT TAATGAGAAA TTGGGTCAGT ATGCTTCCCC GACAGCCAAG 
CGCTGCTGCC AGGATGGGGT GACACGTCTG CCCATGATGC GTTCCTGCGA GCAGCGGGCA 
GCCCGCGTGC AGCAGCCGGA CTGCCGGGAG CCCTTCCTGT CCTGCTGCCA ATTTGCTGAG 
AGTCTGCGCA AGAAGAGCAG GGACAAGGGC CAGGCGGGCC TCCAACGAGC CCTGGAGATC 
CTGCAGGAGG AGGACCTGAT TGATGAGGAT GACATTCCCG TGCGCAGCTT CTTCCCAGAG 
AACTGGCTCT GGAGAGTGGA AACAGTGGAC CGCTTTCAAA TATTGACACT GTGGCTCCCC 
GACTCTCTGA CCACGTGGGA GATCCATGGC CTGAGCCTGT CCAAAACCAA AGGCCTATGT 
GTGGCCACCC CAGTCCAGCT CCGGGTGTTC CGCGAGTTCC ACCTGCACCT CCGCCTGCCC 
ATGTCTGTCC GCCGCTTTGA GCAGCTGGAG CTGCGGCCTG TCCTCTATAA CTACCTGGAT 
AAAAACCTGA CTGTGAGCGT CCACGTGTCC CCAGTGGAGG GGCTGTGCCT GGCTGGGGGC 
GGAGGGCTGG CCCAGCAGGT GCTGGTGCCT GCGGGCTCTG CCCGGCCTGT TGCCTTCTCT 
GTGGTGCCCA CGGCAGCCGC CGCTGTGTCT CTGAAGGTGG TGGCTCGAGG GTCCTTCGAA 
TTCCCTGTGG GAGATGCGGT GTCCAAGGTT CTGCAGATTG AGAAGGAAGG GGCCATCCAT 
AGAGAGGAGC TGGTCTATGA ACTCAACCCC TTGGACCACC GAGGCCGGAC CTTGGAAATA 
CCTGGCAACT CTGATCCCAA TATGATCCCT GATGGGGACT TTAACAGCTA CGTCAGGGTT 
ACAGCCTCAG ATCCATTGGA CACTTTAGGC TCTGAGGGGG CCTTGTCACC AGGAGGCGTG 
GCCTCCCTCT TGAGGCTTCC TCGAGGCTGT GGGGAGCAAA CCATGATCTA CTTGGCTCCG 
ACACTGGCTG CTTCCCGCTA CCTGGACAAG ACAGAGCAGT GGAGCACACT GCCTCCCGAG 
ACCAAGGACC ACGCCGTGGA TCTGATCCAG AAAGGCTACA TGCGGATCCA GCAGTTTCGG 
AAGGCGGATG GTTCCTATGC GGCTTGGTTG TCACGGGACA GCAGCACCTG GCTCACAGCC 
TTTGTGTTGA AGGTCCTGAG TTTGGCCCAG GAGCAGGTAG GAGGCTCGCC TGAGAAACTG 
CAGGAGACAT CTAACTGGCT TCTGTCCCAG CAGCAGGCTG ACGGCTCGTT CCAGGACCCC 
TGTCCAGTGT TAGACAGGAG CATGCAGGGG GGTTTGGTGG GCAATGATGA GACTGTGGCA 
CTCACAGCCT TTGTGACCAT CGCCCTTCAT CATGGGCTGG CCGTCTTCCA GGATGAGGGT 
GCAGAGCCAT TGAAGCAGAG AGTGGAAGCC TCCATCTCAA AGGCAAACTC ATTTTTGGGG 
GAGAAAGCAA GTGCTGGGCT CCTGGGTGCC CACGCAGCTG CCATCACGGC CTATGCCCTG 
ACACTGACCA AGGCGCCTGT GGACCTGCTC GGTGTTGCCC ACAACAACCT CATGGCAATG 
GCCCAGGAGA CTGGAGATAA CCTGTACTGG GGCTCAGTCA CTGGTTCTCA GAGCAATGCC 
GTGTCGCCCA CCCCGGCTCC TCGCAACCCA TCCGACCCCA TGCCCCAGGC CCCAGCCCTG 
TGGATTGAAA CCACAGCCTA CGCCCTGCTG CACCTCCTGC TTCACGAGGG CAAAGCAGAG 
ATGGCAGACC AGGCTTCGGC CTGGCTCACC CGTCAGGGCA GCTTCCAAGG GGGATTCCGC 
AGTACCCAAG ACACGGTGAT TGCCCTGGAT GCCCTGTCTG CCTACTGGAT TGCCTCCCAC 
ACCACTGAGG AGAGGGGTCT CAATGTGACT CTCAGCTCCA CAGGCCGGAA TGGGTTCAAG 
TCCCACGCGC TGCAGCTGAA CAACCGCCAG ATTCGCGGCC TGGAGGAGGA GCTGCAGTTT 
TCCTTGGGCA GCAAGATCAA TGTGAAGGTG GGAGGAAACA GCAAAGGAAC CCTGAAGGTC 
CTTCGTACCT ACAATGTCCT GGACATGAAG AACACGACCT GCCAGGACCT ACAGATAGAA 
GTGACAGTCA AAGGCCACGT CGAGTACACG ATGGAAGCAA ACGAGGACTA TGAGGACTAT 
GAGTACGATG AGCTTCCAGC CAAGGATGAC CCAGATGCCC CTCTGCAGCC CGTGACACCC 
CTGCAGCTGT TTGAGGGTCG GAGGAACCGC CGCAGGAGGG AGGCGCCCAA GGTGGTGGAG 
GAGCAGGAGT CCAGGGTGCA CTACACCGTG TGCATCTGGC GGAACGGCAA GGTGGGGCTG 
TCTGGCATGG CCATCGCGGA CGTCACCCTC CTGAGTGGAT TCCACGCCCT GCGTGCTGAC 
CTGGAGAAGC TGACCTCCCT CTCTGACCGT TACGTGAGTC ACTTTGAGAC CGAGGGGCCC 
CACGTCCTGC TGTATTTTGA CTCGGTCCCC ACCTCCCGGG AGTGCGTGGG CTTTGAGGCT 
GTGCAGGAAG TGCCGGTGGG GCTGGTGCAG CCGGCCAGCG CAACCCTGTA CGACTACTAC 
AACCCCGAGC GCAGATGTTC TGTGTTTTAC GGGGCACCAA GTAAGAGCAG ACTCTTGGCC 
ACCTTGTGTT CTGCTGAAGT CTGCCAGTGT GCTGAGGGGA AGTGCCCTCG CCAGCGTCGC 
GCCCTGGAGC GGGGTCTGCA GGACGAGGAT GGCTACAGGA TGAAGTTTGC CTGCTACTAC 
CCCCGTGTGG AGTACGGCTT CCAGGTTAAG GTTCTCCGAG AAGACAGCAG AGCTGCTTTC 
CGCCTCTTTG AGACCAAGAT CACCCAAGTC CTGCACTTCA CCAAGGATGT CAAGGCCGCT 
GCTAATCAGA TGCGCAACTT CCTGGTTCGA GCCTCCTGCC GCCTTCGCTT GGAACCTGGG 
AAAGAATATT TGATCATGGG TCTGGATGGG GCCACCTATG ACCTCGAGGG ACACCCCCAG 
TACCTGCTGG ACTCGAATAG CTGGATCGAG GAGATGCCCT CTGAACGCCT GTGCCGGAGC 
ACCCGCCAGC GGGCAGCCTG TGCCCAGCTC AACGACTTCC TCCAGGAGTA TGGCACTCAG 
GGGTGCCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C4A ( NM_007293.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_009224.2 Homo sapiens complement C4A (Rodgers blood group) (C4A), transcript variant 1, mRNA.

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CloneID	OHu11432
Clone ID Related Accession (Same CDS sequence)	NM_007293.2 , NM_007293.3
Accession Version	NM_007293.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	complement C4-A isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA304049.1, K02403.1, AL645922.12, BC146673.1 and BC016933.1. On Aug 1, 2019 this sequence version replaced NM_007293.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record represents the C4A*001.1.1 allele. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.237372.1, SRR1803615.258018.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000428956.7/ ENSP00000396688.2 Protein has antimicrobial activity :: PMID: 17132627 RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGCTGC TCTGGGGGCT GATCTGGGCA TCCAGCTTCT TCACCTTATC TCTGCAGAAG 
CCCAGGTTGC TCTTGTTCTC TCCTTCTGTG GTTCATCTGG GGGTCCCCCT ATCGGTGGGG 
GTGCAGCTCC AGGATGTGCC CCGAGGACAG GTAGTGAAAG GATCAGTGTT CCTGAGAAAC 
CCATCTCGTA ATAATGTCCC CTGCTCCCCA AAGGTGGACT TCACCCTTAG CTCAGAAAGA 
GACTTCGCAC TCCTCAGTCT CCAGGTGCCC TTGAAAGATG CGAAGAGCTG TGGCCTCCAT 
CAACTCCTCA GAGGCCCTGA GGTCCAGCTG GTGGCCCATT CGCCATGGCT AAAGGACTCT 
CTGTCCAGAA CGACAAACAT CCAGGGTATC AACCTGCTCT TCTCCTCTCG CCGGGGGCAC 
CTCTTTTTGC AGACGGACCA GCCCATTTAC AACCCTGGCC AGCGGGTTCG GTACCGGGTC 
TTTGCTCTGG ATCAGAAGAT GCGCCCGAGC ACTGACACCA TCACAGTCAT GGTGGAGAAC 
TCTCACGGCC TCCGCGTGCG GAAGAAGGAG GTGTACATGC CCTCGTCCAT CTTCCAGGAT 
GACTTTGTGA TCCCAGACAT CTCAGAGCCA GGGACCTGGA AGATCTCAGC CCGATTCTCA 
GATGGCCTGG AATCCAACAG CAGCACCCAG TTTGAGGTGA AGAAATATGT CCTTCCCAAC 
TTTGAGGTGA AGATCACCCC TGGAAAGCCC TACATCCTGA CGGTGCCAGG CCATCTTGAT 
GAAATGCAGT TAGACATCCA GGCCAGGTAC ATCTATGGGA AGCCAGTGCA GGGGGTGGCA 
TATGTGCGCT TTGGGCTCCT AGATGAGGAT GGTAAGAAGA CTTTCTTTCG GGGGCTGGAG 
AGTCAGACCA AGCTGGTGAA TGGACAGAGC CACATTTCCC TCTCAAAGGC AGAGTTCCAG 
GACGCCCTGG AGAAGCTGAA TATGGGCATT ACTGACCTCC AGGGGCTGCG CCTCTACGTT 
GCTGCAGCCA TCATTGAGTC TCCAGGTGGG GAGATGGAGG AGGCAGAGCT CACATCCTGG 
TATTTTGTGT CATCTCCCTT CTCCTTGGAT CTTAGCAAGA CCAAGCGACA CCTTGTGCCT 
GGGGCCCCCT TCCTGCTGCA GGCCTTGGTC CGTGAGATGT CAGGCTCCCC AGCTTCTGGC 
ATTCCTGTCA AAGTTTCTGC CACGGTGTCT TCTCCTGGGT CTGTTCCTGA AGTCCAGGAC 
ATTCAGCAAA ACACAGACGG GAGCGGCCAA GTCAGCATTC CAATAATTAT CCCTCAGACC 
ATCTCAGAGC TGCAGCTCTC AGTATCTGCA GGCTCCCCAC ATCCAGCGAT AGCCAGGCTC 
ACTGTGGCAG CCCCACCTTC AGGAGGCCCC GGGTTTCTGT CTATTGAGCG GCCGGATTCT 
CGACCTCCTC GTGTTGGGGA CACTCTGAAC CTGAACTTGC GAGCCGTGGG CAGTGGGGCC 
ACCTTTTCTC ATTACTACTA CATGATCCTA TCCCGAGGGC AGATCGTGTT CATGAATCGA 
GAGCCCAAGA GGACCCTGAC CTCGGTCTCG GTGTTTGTGG ACCATCACCT GGCACCCTCC 
TTCTACTTTG TGGCCTTCTA CTACCATGGA GACCACCCAG TGGCCAACTC CCTGCGAGTG 
GATGTCCAGG CTGGGGCCTG CGAGGGCAAG CTGGAGCTCA GCGTGGACGG TGCCAAGCAG 
TACCGGAACG GGGAGTCCGT GAAGCTCCAC TTAGAAACCG ACTCCCTAGC CCTGGTGGCG 
CTGGGAGCCT TGGACACAGC TCTGTATGCT GCAGGCAGCA AGTCCCACAA GCCCCTCAAC 
ATGGGCAAGG TCTTTGAAGC TATGAACAGC TATGACCTCG GCTGTGGTCC TGGGGGTGGG 
GACAGTGCCC TTCAGGTGTT CCAGGCAGCG GGCCTGGCCT TTTCTGATGG AGACCAGTGG 
ACCTTATCCA GAAAGAGACT AAGCTGTCCC AAGGAGAAGA CAACCCGGAA AAAGAGAAAC 
GTGAACTTCC AAAAGGCGAT TAATGAGAAA TTGGGTCAGT ATGCTTCCCC GACAGCCAAG 
CGCTGCTGCC AGGATGGGGT GACACGTCTG CCCATGATGC GTTCCTGCGA GCAGCGGGCA 
GCCCGCGTGC AGCAGCCGGA CTGCCGGGAG CCCTTCCTGT CCTGCTGCCA ATTTGCTGAG 
AGTCTGCGCA AGAAGAGCAG GGACAAGGGC CAGGCGGGCC TCCAACGAGC CCTGGAGATC 
CTGCAGGAGG AGGACCTGAT TGATGAGGAT GACATTCCCG TGCGCAGCTT CTTCCCAGAG 
AACTGGCTCT GGAGAGTGGA AACAGTGGAC CGCTTTCAAA TATTGACACT GTGGCTCCCC 
GACTCTCTGA CCACGTGGGA GATCCATGGC CTGAGCCTGT CCAAAACCAA AGGCCTATGT 
GTGGCCACCC CAGTCCAGCT CCGGGTGTTC CGCGAGTTCC ACCTGCACCT CCGCCTGCCC 
ATGTCTGTCC GCCGCTTTGA GCAGCTGGAG CTGCGGCCTG TCCTCTATAA CTACCTGGAT 
AAAAACCTGA CTGTGAGCGT CCACGTGTCC CCAGTGGAGG GGCTGTGCCT GGCTGGGGGC 
GGAGGGCTGG CCCAGCAGGT GCTGGTGCCT GCGGGCTCTG CCCGGCCTGT TGCCTTCTCT 
GTGGTGCCCA CGGCAGCCGC CGCTGTGTCT CTGAAGGTGG TGGCTCGAGG GTCCTTCGAA 
TTCCCTGTGG GAGATGCGGT GTCCAAGGTT CTGCAGATTG AGAAGGAAGG GGCCATCCAT 
AGAGAGGAGC TGGTCTATGA ACTCAACCCC TTGGACCACC GAGGCCGGAC CTTGGAAATA 
CCTGGCAACT CTGATCCCAA TATGATCCCT GATGGGGACT TTAACAGCTA CGTCAGGGTT 
ACAGCCTCAG ATCCATTGGA CACTTTAGGC TCTGAGGGGG CCTTGTCACC AGGAGGCGTG 
GCCTCCCTCT TGAGGCTTCC TCGAGGCTGT GGGGAGCAAA CCATGATCTA CTTGGCTCCG 
ACACTGGCTG CTTCCCGCTA CCTGGACAAG ACAGAGCAGT GGAGCACACT GCCTCCCGAG 
ACCAAGGACC ACGCCGTGGA TCTGATCCAG AAAGGCTACA TGCGGATCCA GCAGTTTCGG 
AAGGCGGATG GTTCCTATGC GGCTTGGTTG TCACGGGACA GCAGCACCTG GCTCACAGCC 
TTTGTGTTGA AGGTCCTGAG TTTGGCCCAG GAGCAGGTAG GAGGCTCGCC TGAGAAACTG 
CAGGAGACAT CTAACTGGCT TCTGTCCCAG CAGCAGGCTG ACGGCTCGTT CCAGGACCCC 
TGTCCAGTGT TAGACAGGAG CATGCAGGGG GGTTTGGTGG GCAATGATGA GACTGTGGCA 
CTCACAGCCT TTGTGACCAT CGCCCTTCAT CATGGGCTGG CCGTCTTCCA GGATGAGGGT 
GCAGAGCCAT TGAAGCAGAG AGTGGAAGCC TCCATCTCAA AGGCAAACTC ATTTTTGGGG 
GAGAAAGCAA GTGCTGGGCT CCTGGGTGCC CACGCAGCTG CCATCACGGC CTATGCCCTG 
ACACTGACCA AGGCGCCTGT GGACCTGCTC GGTGTTGCCC ACAACAACCT CATGGCAATG 
GCCCAGGAGA CTGGAGATAA CCTGTACTGG GGCTCAGTCA CTGGTTCTCA GAGCAATGCC 
GTGTCGCCCA CCCCGGCTCC TCGCAACCCA TCCGACCCCA TGCCCCAGGC CCCAGCCCTG 
TGGATTGAAA CCACAGCCTA CGCCCTGCTG CACCTCCTGC TTCACGAGGG CAAAGCAGAG 
ATGGCAGACC AGGCTTCGGC CTGGCTCACC CGTCAGGGCA GCTTCCAAGG GGGATTCCGC 
AGTACCCAAG ACACGGTGAT TGCCCTGGAT GCCCTGTCTG CCTACTGGAT TGCCTCCCAC 
ACCACTGAGG AGAGGGGTCT CAATGTGACT CTCAGCTCCA CAGGCCGGAA TGGGTTCAAG 
TCCCACGCGC TGCAGCTGAA CAACCGCCAG ATTCGCGGCC TGGAGGAGGA GCTGCAGTTT 
TCCTTGGGCA GCAAGATCAA TGTGAAGGTG GGAGGAAACA GCAAAGGAAC CCTGAAGGTC 
CTTCGTACCT ACAATGTCCT GGACATGAAG AACACGACCT GCCAGGACCT ACAGATAGAA 
GTGACAGTCA AAGGCCACGT CGAGTACACG ATGGAAGCAA ACGAGGACTA TGAGGACTAT 
GAGTACGATG AGCTTCCAGC CAAGGATGAC CCAGATGCCC CTCTGCAGCC CGTGACACCC 
CTGCAGCTGT TTGAGGGTCG GAGGAACCGC CGCAGGAGGG AGGCGCCCAA GGTGGTGGAG 
GAGCAGGAGT CCAGGGTGCA CTACACCGTG TGCATCTGGC GGAACGGCAA GGTGGGGCTG 
TCTGGCATGG CCATCGCGGA CGTCACCCTC CTGAGTGGAT TCCACGCCCT GCGTGCTGAC 
CTGGAGAAGC TGACCTCCCT CTCTGACCGT TACGTGAGTC ACTTTGAGAC CGAGGGGCCC 
CACGTCCTGC TGTATTTTGA CTCGGTCCCC ACCTCCCGGG AGTGCGTGGG CTTTGAGGCT 
GTGCAGGAAG TGCCGGTGGG GCTGGTGCAG CCGGCCAGCG CAACCCTGTA CGACTACTAC 
AACCCCGAGC GCAGATGTTC TGTGTTTTAC GGGGCACCAA GTAAGAGCAG ACTCTTGGCC 
ACCTTGTGTT CTGCTGAAGT CTGCCAGTGT GCTGAGGGGA AGTGCCCTCG CCAGCGTCGC 
GCCCTGGAGC GGGGTCTGCA GGACGAGGAT GGCTACAGGA TGAAGTTTGC CTGCTACTAC 
CCCCGTGTGG AGTACGGCTT CCAGGTTAAG GTTCTCCGAG AAGACAGCAG AGCTGCTTTC 
CGCCTCTTTG AGACCAAGAT CACCCAAGTC CTGCACTTCA CCAAGGATGT CAAGGCCGCT 
GCTAATCAGA TGCGCAACTT CCTGGTTCGA GCCTCCTGCC GCCTTCGCTT GGAACCTGGG 
AAAGAATATT TGATCATGGG TCTGGATGGG GCCACCTATG ACCTCGAGGG ACACCCCCAG 
TACCTGCTGG ACTCGAATAG CTGGATCGAG GAGATGCCCT CTGAACGCCT GTGCCGGAGC 
ACCCGCCAGC GGGCAGCCTG TGCCCAGCTC AACGACTTCC TCCAGGAGTA TGGCACTCAG 
GGGTGCCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009224.2
CDS	52..5286
Translation	MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAGLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKANSFLGEKASAGLLGAHAAAITAYALTLTKAPVDLLGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQASAWLTRQGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQIRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREDSRAAFRLFETKITQVLHFTKDVKAAANQMRNFLVRASCRLRLEPGKEYLIMGLDGATYDLEGHPQYLLDSNSWIEEMPSERLCRSTRQRAACAQLNDFLQEYGTQGCQV

Target ORF information:

RefSeq Version	NM_007293.3
Organism	Homo sapiens(human)
Definition	Homo sapiens complement C4A (Rodgers blood group) (C4A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007293.3

ORF Insert Sequence:

ATGAGGCTGC TCTGGGGGCT GATCTGGGCA TCCAGCTTCT TCACCTTATC TCTGCAGAAG 
CCCAGGTTGC TCTTGTTCTC TCCTTCTGTG GTTCATCTGG GGGTCCCCCT ATCGGTGGGG 
GTGCAGCTCC AGGATGTGCC CCGAGGACAG GTAGTGAAAG GATCAGTGTT CCTGAGAAAC 
CCATCTCGTA ATAATGTCCC CTGCTCCCCA AAGGTGGACT TCACCCTTAG CTCAGAAAGA 
GACTTCGCAC TCCTCAGTCT CCAGGTGCCC TTGAAAGATG CGAAGAGCTG TGGCCTCCAT 
CAACTCCTCA GAGGCCCTGA GGTCCAGCTG GTGGCCCATT CGCCATGGCT AAAGGACTCT 
CTGTCCAGAA CGACAAACAT CCAGGGTATC AACCTGCTCT TCTCCTCTCG CCGGGGGCAC 
CTCTTTTTGC AGACGGACCA GCCCATTTAC AACCCTGGCC AGCGGGTTCG GTACCGGGTC 
TTTGCTCTGG ATCAGAAGAT GCGCCCGAGC ACTGACACCA TCACAGTCAT GGTGGAGAAC 
TCTCACGGCC TCCGCGTGCG GAAGAAGGAG GTGTACATGC CCTCGTCCAT CTTCCAGGAT 
GACTTTGTGA TCCCAGACAT CTCAGAGCCA GGGACCTGGA AGATCTCAGC CCGATTCTCA 
GATGGCCTGG AATCCAACAG CAGCACCCAG TTTGAGGTGA AGAAATATGT CCTTCCCAAC 
TTTGAGGTGA AGATCACCCC TGGAAAGCCC TACATCCTGA CGGTGCCAGG CCATCTTGAT 
GAAATGCAGT TAGACATCCA GGCCAGGTAC ATCTATGGGA AGCCAGTGCA GGGGGTGGCA 
TATGTGCGCT TTGGGCTCCT AGATGAGGAT GGTAAGAAGA CTTTCTTTCG GGGGCTGGAG 
AGTCAGACCA AGCTGGTGAA TGGACAGAGC CACATTTCCC TCTCAAAGGC AGAGTTCCAG 
GACGCCCTGG AGAAGCTGAA TATGGGCATT ACTGACCTCC AGGGGCTGCG CCTCTACGTT 
GCTGCAGCCA TCATTGAGTC TCCAGGTGGG GAGATGGAGG AGGCAGAGCT CACATCCTGG 
TATTTTGTGT CATCTCCCTT CTCCTTGGAT CTTAGCAAGA CCAAGCGACA CCTTGTGCCT 
GGGGCCCCCT TCCTGCTGCA GGCCTTGGTC CGTGAGATGT CAGGCTCCCC AGCTTCTGGC 
ATTCCTGTCA AAGTTTCTGC CACGGTGTCT TCTCCTGGGT CTGTTCCTGA AGTCCAGGAC 
ATTCAGCAAA ACACAGACGG GAGCGGCCAA GTCAGCATTC CAATAATTAT CCCTCAGACC 
ATCTCAGAGC TGCAGCTCTC AGTATCTGCA GGCTCCCCAC ATCCAGCGAT AGCCAGGCTC 
ACTGTGGCAG CCCCACCTTC AGGAGGCCCC GGGTTTCTGT CTATTGAGCG GCCGGATTCT 
CGACCTCCTC GTGTTGGGGA CACTCTGAAC CTGAACTTGC GAGCCGTGGG CAGTGGGGCC 
ACCTTTTCTC ATTACTACTA CATGATCCTA TCCCGAGGGC AGATCGTGTT CATGAATCGA 
GAGCCCAAGA GGACCCTGAC CTCGGTCTCG GTGTTTGTGG ACCATCACCT GGCACCCTCC 
TTCTACTTTG TGGCCTTCTA CTACCATGGA GACCACCCAG TGGCCAACTC CCTGCGAGTG 
GATGTCCAGG CTGGGGCCTG CGAGGGCAAG CTGGAGCTCA GCGTGGACGG TGCCAAGCAG 
TACCGGAACG GGGAGTCCGT GAAGCTCCAC TTAGAAACCG ACTCCCTAGC CCTGGTGGCG 
CTGGGAGCCT TGGACACAGC TCTGTATGCT GCAGGCAGCA AGTCCCACAA GCCCCTCAAC 
ATGGGCAAGG TCTTTGAAGC TATGAACAGC TATGACCTCG GCTGTGGTCC TGGGGGTGGG 
GACAGTGCCC TTCAGGTGTT CCAGGCAGCG GGCCTGGCCT TTTCTGATGG AGACCAGTGG 
ACCTTATCCA GAAAGAGACT AAGCTGTCCC AAGGAGAAGA CAACCCGGAA AAAGAGAAAC 
GTGAACTTCC AAAAGGCGAT TAATGAGAAA TTGGGTCAGT ATGCTTCCCC GACAGCCAAG 
CGCTGCTGCC AGGATGGGGT GACACGTCTG CCCATGATGC GTTCCTGCGA GCAGCGGGCA 
GCCCGCGTGC AGCAGCCGGA CTGCCGGGAG CCCTTCCTGT CCTGCTGCCA ATTTGCTGAG 
AGTCTGCGCA AGAAGAGCAG GGACAAGGGC CAGGCGGGCC TCCAACGAGC CCTGGAGATC 
CTGCAGGAGG AGGACCTGAT TGATGAGGAT GACATTCCCG TGCGCAGCTT CTTCCCAGAG 
AACTGGCTCT GGAGAGTGGA AACAGTGGAC CGCTTTCAAA TATTGACACT GTGGCTCCCC 
GACTCTCTGA CCACGTGGGA GATCCATGGC CTGAGCCTGT CCAAAACCAA AGGCCTATGT 
GTGGCCACCC CAGTCCAGCT CCGGGTGTTC CGCGAGTTCC ACCTGCACCT CCGCCTGCCC 
ATGTCTGTCC GCCGCTTTGA GCAGCTGGAG CTGCGGCCTG TCCTCTATAA CTACCTGGAT 
AAAAACCTGA CTGTGAGCGT CCACGTGTCC CCAGTGGAGG GGCTGTGCCT GGCTGGGGGC 
GGAGGGCTGG CCCAGCAGGT GCTGGTGCCT GCGGGCTCTG CCCGGCCTGT TGCCTTCTCT 
GTGGTGCCCA CGGCAGCCGC CGCTGTGTCT CTGAAGGTGG TGGCTCGAGG GTCCTTCGAA 
TTCCCTGTGG GAGATGCGGT GTCCAAGGTT CTGCAGATTG AGAAGGAAGG GGCCATCCAT 
AGAGAGGAGC TGGTCTATGA ACTCAACCCC TTGGACCACC GAGGCCGGAC CTTGGAAATA 
CCTGGCAACT CTGATCCCAA TATGATCCCT GATGGGGACT TTAACAGCTA CGTCAGGGTT 
ACAGCCTCAG ATCCATTGGA CACTTTAGGC TCTGAGGGGG CCTTGTCACC AGGAGGCGTG 
GCCTCCCTCT TGAGGCTTCC TCGAGGCTGT GGGGAGCAAA CCATGATCTA CTTGGCTCCG 
ACACTGGCTG CTTCCCGCTA CCTGGACAAG ACAGAGCAGT GGAGCACACT GCCTCCCGAG 
ACCAAGGACC ACGCCGTGGA TCTGATCCAG AAAGGCTACA TGCGGATCCA GCAGTTTCGG 
AAGGCGGATG GTTCCTATGC GGCTTGGTTG TCACGGGACA GCAGCACCTG GCTCACAGCC 
TTTGTGTTGA AGGTCCTGAG TTTGGCCCAG GAGCAGGTAG GAGGCTCGCC TGAGAAACTG 
CAGGAGACAT CTAACTGGCT TCTGTCCCAG CAGCAGGCTG ACGGCTCGTT CCAGGACCCC 
TGTCCAGTGT TAGACAGGAG CATGCAGGGG GGTTTGGTGG GCAATGATGA GACTGTGGCA 
CTCACAGCCT TTGTGACCAT CGCCCTTCAT CATGGGCTGG CCGTCTTCCA GGATGAGGGT 
GCAGAGCCAT TGAAGCAGAG AGTGGAAGCC TCCATCTCAA AGGCAAACTC ATTTTTGGGG 
GAGAAAGCAA GTGCTGGGCT CCTGGGTGCC CACGCAGCTG CCATCACGGC CTATGCCCTG 
ACACTGACCA AGGCGCCTGT GGACCTGCTC GGTGTTGCCC ACAACAACCT CATGGCAATG 
GCCCAGGAGA CTGGAGATAA CCTGTACTGG GGCTCAGTCA CTGGTTCTCA GAGCAATGCC 
GTGTCGCCCA CCCCGGCTCC TCGCAACCCA TCCGACCCCA TGCCCCAGGC CCCAGCCCTG 
TGGATTGAAA CCACAGCCTA CGCCCTGCTG CACCTCCTGC TTCACGAGGG CAAAGCAGAG 
ATGGCAGACC AGGCTTCGGC CTGGCTCACC CGTCAGGGCA GCTTCCAAGG GGGATTCCGC 
AGTACCCAAG ACACGGTGAT TGCCCTGGAT GCCCTGTCTG CCTACTGGAT TGCCTCCCAC 
ACCACTGAGG AGAGGGGTCT CAATGTGACT CTCAGCTCCA CAGGCCGGAA TGGGTTCAAG 
TCCCACGCGC TGCAGCTGAA CAACCGCCAG ATTCGCGGCC TGGAGGAGGA GCTGCAGTTT 
TCCTTGGGCA GCAAGATCAA TGTGAAGGTG GGAGGAAACA GCAAAGGAAC CCTGAAGGTC 
CTTCGTACCT ACAATGTCCT GGACATGAAG AACACGACCT GCCAGGACCT ACAGATAGAA 
GTGACAGTCA AAGGCCACGT CGAGTACACG ATGGAAGCAA ACGAGGACTA TGAGGACTAT 
GAGTACGATG AGCTTCCAGC CAAGGATGAC CCAGATGCCC CTCTGCAGCC CGTGACACCC 
CTGCAGCTGT TTGAGGGTCG GAGGAACCGC CGCAGGAGGG AGGCGCCCAA GGTGGTGGAG 
GAGCAGGAGT CCAGGGTGCA CTACACCGTG TGCATCTGGC GGAACGGCAA GGTGGGGCTG 
TCTGGCATGG CCATCGCGGA CGTCACCCTC CTGAGTGGAT TCCACGCCCT GCGTGCTGAC 
CTGGAGAAGC TGACCTCCCT CTCTGACCGT TACGTGAGTC ACTTTGAGAC CGAGGGGCCC 
CACGTCCTGC TGTATTTTGA CTCGGTCCCC ACCTCCCGGG AGTGCGTGGG CTTTGAGGCT 
GTGCAGGAAG TGCCGGTGGG GCTGGTGCAG CCGGCCAGCG CAACCCTGTA CGACTACTAC 
AACCCCGAGC GCAGATGTTC TGTGTTTTAC GGGGCACCAA GTAAGAGCAG ACTCTTGGCC 
ACCTTGTGTT CTGCTGAAGT CTGCCAGTGT GCTGAGGGGA AGTGCCCTCG CCAGCGTCGC 
GCCCTGGAGC GGGGTCTGCA GGACGAGGAT GGCTACAGGA TGAAGTTTGC CTGCTACTAC 
CCCCGTGTGG AGTACGGCTT CCAGGTTAAG GTTCTCCGAG AAGACAGCAG AGCTGCTTTC 
CGCCTCTTTG AGACCAAGAT CACCCAAGTC CTGCACTTCA CCAAGGATGT CAAGGCCGCT 
GCTAATCAGA TGCGCAACTT CCTGGTTCGA GCCTCCTGCC GCCTTCGCTT GGAACCTGGG 
AAAGAATATT TGATCATGGG TCTGGATGGG GCCACCTATG ACCTCGAGGG ACACCCCCAG 
TACCTGCTGG ACTCGAATAG CTGGATCGAG GAGATGCCCT CTGAACGCCT GTGCCGGAGC 
ACCCGCCAGC GGGCAGCCTG TGCCCAGCTC AACGACTTCC TCCAGGAGTA TGGCACTCAG 
GGGTGCCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C4A ( XM_017030289.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885778.1 Homo sapiens complement component 4A (Rodgers blood group) (C4A), transcript variant X1, mRNA.

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CloneID	OHu99646
Clone ID Related Accession (Same CDS sequence)	XM_017030289.1
Accession Version	XM_017030289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	complement C4-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167247.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTGC TCTGGGGGCT GATCTGGGCA TCCAGCTTCT TCACCTTATC TCTGCAGAAG 
CCCAGGTTGC TCTTGTTCTC TCCTTCTGTG GTTCATCTGG GGGTCCCCCT ATCGGTGGGG 
GTGCAGCTCC AGGATGTGCC CCGAGGACAG GTAGTGAAAG GATCAGTGTT CCTGAGAAAC 
CCATCTCGTA ATAATGTCCC CTGCTCCCCA AAGGTGGACT TCACCCTTAG CTCAGAAAGA 
GACTTCGCAC TCCTCAGTCT CCAGGTGCCC TTGAAAGATG CGAAGAGCTG TGGCCTCCAT 
CAACTCCTCA GAGGCCCTGA GGTCCAGCTG GTGGCCCATT CGCCATGGCT AAAGGACTCT 
CTGTCCAGAA CGACAAACAT CCAGGGTATC AACCTGCTCT TCTCCTCTCG CCGGGGGCAC 
CTCTTTTTGC AGACGGACCA GCCCATTTAC AACCCTGGCC AGCGGGTTCG GTACCGGGTC 
TTTGCTCTGG ATCAGAAGAT GCGCCCGAGC ACTGACACCA TCACAGTCAT GGTGGAGAAC 
TCTCACGGCC TCCGCGTGCG GAAGAAGGAG GTGTACATGC CCTCGTCCAT CTTCCAGGAT 
GACTTTGTGA TCCCAGACAT CTCAGAGCCA GGGACCTGGA AGATCTCAGC CCGATTCTCA 
GATGGCCTGG AATCCAACAG CAGCACCCAG TTTGAGGTGA AGAAATATGT CCTTCCCAAC 
TTTGAGGTGA AGATCACCCC TGGAAAGCCC TACATCCTGA CGGTGCCAGG CCATCTTGAT 
GAAATGCAGT TAGACATCCA GGCCAGGTAC ATCTATGGGA AGCCAGTGCA GGGGGTGGCA 
TATGTGCGCT TTGGGCTCCT AGATGAGGAT GGTAAGAAGA CTTTCTTTCG GGGGCTGGAG 
AGTCAGACCA AGCTGGTGAA TGGACAGAGC CACATTTCCC TCTCAAAGGC AGAGTTCCAG 
GACGCCCTGG AGAAGCTGAA TATGGGCATT ACTGACCTCC AGGGGCTGCG CCTCTACGTT 
GCTGCAGCCA TCATTGAGTA TCCAGGTGGG GAGATGGAGG AGGCAGAGCT CACATCCTGG 
TATTTTGTGT CATCTCCCTT CTCCTTGGAT CTTAGCAAGA CCAAGCGACA CCTTGTGCCT 
GGGGCCCCCT TCCTGCTGCA GGCCTTGGTC CGTGAGATGT CAGGCTCCCC AGCTTCTGGC 
ATTCCTGTCA AAGTTTCTGC CACGGTGTCT TCTCCTGGGT CTGTTCCTGA AGTCCAGGAC 
ATTCAGCAAA ACACAGACGG GAGCGGCCAA GTCAGCATTC CAATAATTAT CCCTCAGACC 
ATCTCAGAGC TGCAGCTCTC AGTATCTGCA GGCTCCCCAC ATCCAGCGAT AGCCAGGCTC 
ACTGTGGCAG CCCCACCTTC AGGAGGCCCC GGGTTTCTGT CTATTGAGCG GCCGGATTCT 
CGACCTCCTC GTGTTGGGGA CACTCTGAAC CTGAACTTGC GAGCCGTGGG CAGTGGGGCC 
ACCTTTTCTC ATTACTACTA CATGATCCTA TCCCGAGGGC AGATCGTGTT CATGAATCGA 
GAGCCCAAGA GGACCCTGAC CTCGGTCTCG GTGTTTGTGG ACCATCACCT GGCACCCTCC 
TTCTACTTTG TGGCCTTCTA CTACCATGGA GACCACCCAG TGGCCAACTC CCTGCGAGTG 
GATGTCCAGG CTGGGGCCTG CGAGGGCAAG CTGGAGCTCA GCGTGGACGG TGCCAAGCAG 
TACCGGAACG GGGAGTCCGT GAAGCTCCAC TTAGAAACCG ACTCCCTAGC CCTGGTGGCG 
CTGGGAGCCT TGGACACAGC TCTGTATGCT GCAGGCAGCA AGTCCCACAA GCCCCTCAAC 
ATGGGCAAGG TCTTTGAAGC TATGAACAGC TATGACCTCG GCTGTGGTCC TGGGGGTGGG 
GACAGTGCCC TTCAGGTGTT CCAGGCAGCG GGCCTGGCCT TTTCTGATGG AGACCAGTGG 
ACCTTATCCA GAAAGAGACT AAGCTGTCCC AAGGAGAAGA CAACCCGGAA AAAGAGAAAC 
GTGAACTTCC AAAAGGCGAT TAATGAGAAA TTGGGTCAGT ATGCTTCCCC GACAGCCAAG 
CGCTGCTGCC AGGATGGGGT GACACGTCTG CCCATGATGC GTTCCTGCGA GCAGCGGGCA 
GCCCGCGTGC AGCAGCCGGA CTGCCGGGAG CCCTTCCTGT CCTGCTGCCA ATTTGCTGAG 
AGTCTGCGCA AGAAGAGCAG GGACAAGGGC CAGGCGGGCC TCCAACGAGC CCTGGAGATC 
CTGCAGGAGG AGGACCTGAT TGATGAGGAT GACATTCCCG TGCGCAGCTT CTTCCCAGAG 
AACTGGCTCT GGAGAGTGGA AACAGTGGAC CGCTTTCAAA TATTGACACT GTGGCTCCCC 
GACTCTCTGA CCACGTGGGA GATCCATGGC CTGAGCCTGT CCAAAACCAA AGGCCTATGT 
GTGGCCACCC CAGTCCAGCT CCGGGTGTTC CGCGAGTTCC ACCTGCACCT CCGCCTGCCC 
ATGTCTGTCC GCCGCTTTGA GCAGCTGGAG CTGCGGCCTG TCCTCTATAA CTACCTGGAT 
AAAAACCTGA CTGTGAGCGT CCACGTGTCC CCAGTGGAGG GGCTGTGCCT GGCTGGGGGC 
GGAGGGCTGG CCCAGCAGGT GCTGGTGCCT GCGGGCTCTG CCCGGCCTGT TGCCTTCTCT 
GTGGTGCCCA CGGCAGCCGC CGCTGTGTCT CTGAAGGTGG TGGCTCGAGG GTCCTTCGAA 
TTCCCTGTGG GAGATGCGGT GTCCAAGGTT CTGCAGATTG AGAAGGAAGG GGCCATCCAT 
AGAGAGGAGC TGGTCTATGA ACTCAACCCC TTGGACCACC GAGGCCGGAC CTTGGAAATA 
CCTGGCAACT CTGATCCCAA TATGATCCCT GATGGGGACT TTAACAGCTA CGTCAGGGTT 
ACAGCCTCAG ATCCATTGGA CACTTTAGGC TCTGAGGGGG CCTTGTCACC AGGAGGCGTG 
GCCTCCCTCT TGAGGCTTCC TCGAGGCTGT GGGGAGCAAA CCATGATCTA CTTGGCTCCG 
ACACTGGCTG CTTCCCGCTA CCTGGACAAG ACAGAGCAGT GGAGCACACT GCCTCCCGAG 
ACCAAGGACC ACGCCGTGGA TCTGATCCAG AAAGGCTACA TGCGGATCCA GCAGTTTCGG 
AAGGCGGATG GTTCCTATGC GGCTTGGTTG TCACGGGACA GCAGCACCTG GCTCACAGCC 
TTTGTGTTGA AGGTCCTGAG TTTGGCCCAG GAGCAGGTAG GAGGCTCGCC TGAGAAACTG 
CAGGAGACAT CTAACTGGCT TCTGTCCCAG CAGCAGGCTG ACGGCTCGTT CCAGGACCCC 
TGTCCAGTGT TAGACAGGAG CATGCAGGGG GGTTTGGTGG GCAATGATGA GACTGTGGCA 
CTCACAGCCT TTGTGACCAT CGCCCTTCAT CATGGGCTGG CCGTCTTCCA GGATGAGGGT 
GCAGAGCCAT TGAAGCAGAG AGTGGAAGCC TCCATCTCAA AGGCAAACTC ATTTTTGGGG 
GAGAAAGCAA GTGCTGGGCT CCTGGGTGCC CACGCAGCTG CCATCACGGC CTATGCCCTG 
ACACTGACCA AGGCGCCTGT GGACCTGCTC GGTGTTGCCC ACAACAACCT CATGGCAATG 
GCCCAGGAGA CTGGAGATAA CCTGTACTGG GGCTCAGTCA CTGGTTCTCA GAGCAATGCC 
GTGTCGCCCA CCCCGGCTCC TCGCAACCCA TCCGACCCCA TGCCCCAGGC CCCAGCCCTG 
TGGATTGAAA CCACAGCCTA CGCCCTGCTG CACCTCCTGC TTCACGAGGG CAAAGCAGAG 
ATGGCAGACC AGGCTGCGGC CTGGCTCACC CGTCAGGGCA GCTTCCAAGG GGGATTCCGC 
AGTACCCAAG ACACGGTGAT TGCCCTGGAT GCCCTGTCTG CCTACTGGAT TGCCTCCCAC 
ACCACTGAGG AGAGGGGTCT CAATGTGACT CTCAGCTCCA CAGGCCGGAA TGGGTTCAAG 
TCCCACGCGC TGCAGCTGAA CAACCGCCAG ATTCGCGGCC TGGAGGAGGA GCTGCAGTTT 
TCCTTGGGCA GCAAGATCAA TGTGAAGGTG GGAGGAAACA GCAAAGGAAC CCTGAAGGTC 
CTTCGTACCT ACAATGTCCT GGACATGAAG AACACGACCT GCCAGGACCT ACAGATAGAA 
GTGACAGTCA AAGGCCACGT CGAGTACACG ATGGAAGCAA ACGAGGACTA TGAGGACTAT 
GAGTACGATG AGCTTCCAGC CAAGGATGAC CCAGATGCCC CTCTGCAGCC CGTGACACCC 
CTGCAGCTGT TTGAGGGTCG GAGGAACCGC CGCAGGAGGG AGGCGCCCAA GGTGGTGGAG 
GAGCAGGAGT CCAGGGTGCA CTACACCGTG TGCATCTGGC GGAACGGCAA GGTGGGGCTG 
TCTGGCATGG CCATCGCGGA CGTCACCCTC CTGAGTGGAT TCCACGCCCT GCGTGCTGAC 
CTGGAGAAGC TGACCTCCCT CTCTGACCGT TACGTGAGTC ACTTTGAGAC CGAGGGGCCC 
CACGTCCTGC TGTATTTTGA CTCGGTCCCC ACCTCCCGGG AGTGCGTGGG CTTTGAGGCT 
GTGCAGGAAG TGCCGGTGGG GCTGGTGCAG CCGGCCAGCG CAACCCTGTA CGACTACTAC 
AACCCCGAGC GCAGATGTTC TGTGTTTTAC GGGGCACCAA GTAAGAGCAG ACTCTTGGCC 
ACCTTGTGTT CTGCTGAAGT CTGCCAGTGT GCTGAGGGGA AGTGCCCTCG CCAGCGTCGC 
GCCCTGGAGC GGGGTCTGCA GGACGAGGAT GGCTACAGGA TGAAGTTTGC CTGCTACTAC 
CCCCGTGTGG AGTACGGCTT CCAGGTTAAG GTTCTCCGAG AAGACAGCAG AGCTGCTTTC 
CGCCTCTTTG AGACCAAGAT CACCCAAGTC CTGCACTTCA CCAAGGATGT CAAGGCCGCT 
GCTAATCAGA TGCGCAACTT CCTGGTTCGA GCCTCCTGCC GCCTTCGCTT GGAACCTGGG 
AAAGAATATT TGATCATGGG TCTAGATGGG GCCACCTATG ACCTCGAGGG ACACCCCCAG 
TACCTGCTGG ACTCGAATAG CTGGATCGAG GAGATGCCCT CTGAACGCCT GTGCCGGAGC 
ACCCGCCAGC GGGCAGCCTG TGCCCAGCTC AACGACTTCC TCCAGGAGTA TGGCACTCAG 
GGGTGCCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885778.1
CDS	79..5313
Translation	MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIEYPGGEMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAGLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKANSFLGEKASAGLLGAHAAAITAYALTLTKAPVDLLGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQIRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREDSRAAFRLFETKITQVLHFTKDVKAAANQMRNFLVRASCRLRLEPGKEYLIMGLDGATYDLEGHPQYLLDSNSWIEEMPSERLCRSTRQRAACAQLNDFLQEYGTQGCQV

Target ORF information:

RefSeq Version	XM_017030289.1
Organism	Homo sapiens(human)
Definition	Homo sapiens complement component 4A (Rodgers blood group) (C4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017030289.1

ORF Insert Sequence:

ATGAGGCTGC TCTGGGGGCT GATCTGGGCA TCCAGCTTCT TCACCTTATC TCTGCAGAAG 
CCCAGGTTGC TCTTGTTCTC TCCTTCTGTG GTTCATCTGG GGGTCCCCCT ATCGGTGGGG 
GTGCAGCTCC AGGATGTGCC CCGAGGACAG GTAGTGAAAG GATCAGTGTT CCTGAGAAAC 
CCATCTCGTA ATAATGTCCC CTGCTCCCCA AAGGTGGACT TCACCCTTAG CTCAGAAAGA 
GACTTCGCAC TCCTCAGTCT CCAGGTGCCC TTGAAAGATG CGAAGAGCTG TGGCCTCCAT 
CAACTCCTCA GAGGCCCTGA GGTCCAGCTG GTGGCCCATT CGCCATGGCT AAAGGACTCT 
CTGTCCAGAA CGACAAACAT CCAGGGTATC AACCTGCTCT TCTCCTCTCG CCGGGGGCAC 
CTCTTTTTGC AGACGGACCA GCCCATTTAC AACCCTGGCC AGCGGGTTCG GTACCGGGTC 
TTTGCTCTGG ATCAGAAGAT GCGCCCGAGC ACTGACACCA TCACAGTCAT GGTGGAGAAC 
TCTCACGGCC TCCGCGTGCG GAAGAAGGAG GTGTACATGC CCTCGTCCAT CTTCCAGGAT 
GACTTTGTGA TCCCAGACAT CTCAGAGCCA GGGACCTGGA AGATCTCAGC CCGATTCTCA 
GATGGCCTGG AATCCAACAG CAGCACCCAG TTTGAGGTGA AGAAATATGT CCTTCCCAAC 
TTTGAGGTGA AGATCACCCC TGGAAAGCCC TACATCCTGA CGGTGCCAGG CCATCTTGAT 
GAAATGCAGT TAGACATCCA GGCCAGGTAC ATCTATGGGA AGCCAGTGCA GGGGGTGGCA 
TATGTGCGCT TTGGGCTCCT AGATGAGGAT GGTAAGAAGA CTTTCTTTCG GGGGCTGGAG 
AGTCAGACCA AGCTGGTGAA TGGACAGAGC CACATTTCCC TCTCAAAGGC AGAGTTCCAG 
GACGCCCTGG AGAAGCTGAA TATGGGCATT ACTGACCTCC AGGGGCTGCG CCTCTACGTT 
GCTGCAGCCA TCATTGAGTA TCCAGGTGGG GAGATGGAGG AGGCAGAGCT CACATCCTGG 
TATTTTGTGT CATCTCCCTT CTCCTTGGAT CTTAGCAAGA CCAAGCGACA CCTTGTGCCT 
GGGGCCCCCT TCCTGCTGCA GGCCTTGGTC CGTGAGATGT CAGGCTCCCC AGCTTCTGGC 
ATTCCTGTCA AAGTTTCTGC CACGGTGTCT TCTCCTGGGT CTGTTCCTGA AGTCCAGGAC 
ATTCAGCAAA ACACAGACGG GAGCGGCCAA GTCAGCATTC CAATAATTAT CCCTCAGACC 
ATCTCAGAGC TGCAGCTCTC AGTATCTGCA GGCTCCCCAC ATCCAGCGAT AGCCAGGCTC 
ACTGTGGCAG CCCCACCTTC AGGAGGCCCC GGGTTTCTGT CTATTGAGCG GCCGGATTCT 
CGACCTCCTC GTGTTGGGGA CACTCTGAAC CTGAACTTGC GAGCCGTGGG CAGTGGGGCC 
ACCTTTTCTC ATTACTACTA CATGATCCTA TCCCGAGGGC AGATCGTGTT CATGAATCGA 
GAGCCCAAGA GGACCCTGAC CTCGGTCTCG GTGTTTGTGG ACCATCACCT GGCACCCTCC 
TTCTACTTTG TGGCCTTCTA CTACCATGGA GACCACCCAG TGGCCAACTC CCTGCGAGTG 
GATGTCCAGG CTGGGGCCTG CGAGGGCAAG CTGGAGCTCA GCGTGGACGG TGCCAAGCAG 
TACCGGAACG GGGAGTCCGT GAAGCTCCAC TTAGAAACCG ACTCCCTAGC CCTGGTGGCG 
CTGGGAGCCT TGGACACAGC TCTGTATGCT GCAGGCAGCA AGTCCCACAA GCCCCTCAAC 
ATGGGCAAGG TCTTTGAAGC TATGAACAGC TATGACCTCG GCTGTGGTCC TGGGGGTGGG 
GACAGTGCCC TTCAGGTGTT CCAGGCAGCG GGCCTGGCCT TTTCTGATGG AGACCAGTGG 
ACCTTATCCA GAAAGAGACT AAGCTGTCCC AAGGAGAAGA CAACCCGGAA AAAGAGAAAC 
GTGAACTTCC AAAAGGCGAT TAATGAGAAA TTGGGTCAGT ATGCTTCCCC GACAGCCAAG 
CGCTGCTGCC AGGATGGGGT GACACGTCTG CCCATGATGC GTTCCTGCGA GCAGCGGGCA 
GCCCGCGTGC AGCAGCCGGA CTGCCGGGAG CCCTTCCTGT CCTGCTGCCA ATTTGCTGAG 
AGTCTGCGCA AGAAGAGCAG GGACAAGGGC CAGGCGGGCC TCCAACGAGC CCTGGAGATC 
CTGCAGGAGG AGGACCTGAT TGATGAGGAT GACATTCCCG TGCGCAGCTT CTTCCCAGAG 
AACTGGCTCT GGAGAGTGGA AACAGTGGAC CGCTTTCAAA TATTGACACT GTGGCTCCCC 
GACTCTCTGA CCACGTGGGA GATCCATGGC CTGAGCCTGT CCAAAACCAA AGGCCTATGT 
GTGGCCACCC CAGTCCAGCT CCGGGTGTTC CGCGAGTTCC ACCTGCACCT CCGCCTGCCC 
ATGTCTGTCC GCCGCTTTGA GCAGCTGGAG CTGCGGCCTG TCCTCTATAA CTACCTGGAT 
AAAAACCTGA CTGTGAGCGT CCACGTGTCC CCAGTGGAGG GGCTGTGCCT GGCTGGGGGC 
GGAGGGCTGG CCCAGCAGGT GCTGGTGCCT GCGGGCTCTG CCCGGCCTGT TGCCTTCTCT 
GTGGTGCCCA CGGCAGCCGC CGCTGTGTCT CTGAAGGTGG TGGCTCGAGG GTCCTTCGAA 
TTCCCTGTGG GAGATGCGGT GTCCAAGGTT CTGCAGATTG AGAAGGAAGG GGCCATCCAT 
AGAGAGGAGC TGGTCTATGA ACTCAACCCC TTGGACCACC GAGGCCGGAC CTTGGAAATA 
CCTGGCAACT CTGATCCCAA TATGATCCCT GATGGGGACT TTAACAGCTA CGTCAGGGTT 
ACAGCCTCAG ATCCATTGGA CACTTTAGGC TCTGAGGGGG CCTTGTCACC AGGAGGCGTG 
GCCTCCCTCT TGAGGCTTCC TCGAGGCTGT GGGGAGCAAA CCATGATCTA CTTGGCTCCG 
ACACTGGCTG CTTCCCGCTA CCTGGACAAG ACAGAGCAGT GGAGCACACT GCCTCCCGAG 
ACCAAGGACC ACGCCGTGGA TCTGATCCAG AAAGGCTACA TGCGGATCCA GCAGTTTCGG 
AAGGCGGATG GTTCCTATGC GGCTTGGTTG TCACGGGACA GCAGCACCTG GCTCACAGCC 
TTTGTGTTGA AGGTCCTGAG TTTGGCCCAG GAGCAGGTAG GAGGCTCGCC TGAGAAACTG 
CAGGAGACAT CTAACTGGCT TCTGTCCCAG CAGCAGGCTG ACGGCTCGTT CCAGGACCCC 
TGTCCAGTGT TAGACAGGAG CATGCAGGGG GGTTTGGTGG GCAATGATGA GACTGTGGCA 
CTCACAGCCT TTGTGACCAT CGCCCTTCAT CATGGGCTGG CCGTCTTCCA GGATGAGGGT 
GCAGAGCCAT TGAAGCAGAG AGTGGAAGCC TCCATCTCAA AGGCAAACTC ATTTTTGGGG 
GAGAAAGCAA GTGCTGGGCT CCTGGGTGCC CACGCAGCTG CCATCACGGC CTATGCCCTG 
ACACTGACCA AGGCGCCTGT GGACCTGCTC GGTGTTGCCC ACAACAACCT CATGGCAATG 
GCCCAGGAGA CTGGAGATAA CCTGTACTGG GGCTCAGTCA CTGGTTCTCA GAGCAATGCC 
GTGTCGCCCA CCCCGGCTCC TCGCAACCCA TCCGACCCCA TGCCCCAGGC CCCAGCCCTG 
TGGATTGAAA CCACAGCCTA CGCCCTGCTG CACCTCCTGC TTCACGAGGG CAAAGCAGAG 
ATGGCAGACC AGGCTGCGGC CTGGCTCACC CGTCAGGGCA GCTTCCAAGG GGGATTCCGC 
AGTACCCAAG ACACGGTGAT TGCCCTGGAT GCCCTGTCTG CCTACTGGAT TGCCTCCCAC 
ACCACTGAGG AGAGGGGTCT CAATGTGACT CTCAGCTCCA CAGGCCGGAA TGGGTTCAAG 
TCCCACGCGC TGCAGCTGAA CAACCGCCAG ATTCGCGGCC TGGAGGAGGA GCTGCAGTTT 
TCCTTGGGCA GCAAGATCAA TGTGAAGGTG GGAGGAAACA GCAAAGGAAC CCTGAAGGTC 
CTTCGTACCT ACAATGTCCT GGACATGAAG AACACGACCT GCCAGGACCT ACAGATAGAA 
GTGACAGTCA AAGGCCACGT CGAGTACACG ATGGAAGCAA ACGAGGACTA TGAGGACTAT 
GAGTACGATG AGCTTCCAGC CAAGGATGAC CCAGATGCCC CTCTGCAGCC CGTGACACCC 
CTGCAGCTGT TTGAGGGTCG GAGGAACCGC CGCAGGAGGG AGGCGCCCAA GGTGGTGGAG 
GAGCAGGAGT CCAGGGTGCA CTACACCGTG TGCATCTGGC GGAACGGCAA GGTGGGGCTG 
TCTGGCATGG CCATCGCGGA CGTCACCCTC CTGAGTGGAT TCCACGCCCT GCGTGCTGAC 
CTGGAGAAGC TGACCTCCCT CTCTGACCGT TACGTGAGTC ACTTTGAGAC CGAGGGGCCC 
CACGTCCTGC TGTATTTTGA CTCGGTCCCC ACCTCCCGGG AGTGCGTGGG CTTTGAGGCT 
GTGCAGGAAG TGCCGGTGGG GCTGGTGCAG CCGGCCAGCG CAACCCTGTA CGACTACTAC 
AACCCCGAGC GCAGATGTTC TGTGTTTTAC GGGGCACCAA GTAAGAGCAG ACTCTTGGCC 
ACCTTGTGTT CTGCTGAAGT CTGCCAGTGT GCTGAGGGGA AGTGCCCTCG CCAGCGTCGC 
GCCCTGGAGC GGGGTCTGCA GGACGAGGAT GGCTACAGGA TGAAGTTTGC CTGCTACTAC 
CCCCGTGTGG AGTACGGCTT CCAGGTTAAG GTTCTCCGAG AAGACAGCAG AGCTGCTTTC 
CGCCTCTTTG AGACCAAGAT CACCCAAGTC CTGCACTTCA CCAAGGATGT CAAGGCCGCT 
GCTAATCAGA TGCGCAACTT CCTGGTTCGA GCCTCCTGCC GCCTTCGCTT GGAACCTGGG 
AAAGAATATT TGATCATGGG TCTAGATGGG GCCACCTATG ACCTCGAGGG ACACCCCCAG 
TACCTGCTGG ACTCGAATAG CTGGATCGAG GAGATGCCCT CTGAACGCCT GTGCCGGAGC 
ACCCGCCAGC GGGCAGCCTG TGCCCAGCTC AACGACTTCC TCCAGGAGTA TGGCACTCAG 
GGGTGCCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C4A ( NM_001252204.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001239133.1 Homo sapiens complement C4A (Rodgers blood group) (C4A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10491
Clone ID Related Accession (Same CDS sequence)	NM_001252204.1
Accession Version	NM_001252204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	complement C4-A isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA304049.1, K02403.1, AL645922.12 and BC146673.1. On or before Jul 26, 2012 this sequence version replaced XM_003846465.1, XM_003846466.1. Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC146673.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 17132627 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGCTGC TCTGGGGGCT GATCTGGGCA TCCAGCTTCT TCACCTTATC TCTGCAGAAG 
CCCAGGTTGC TCTTGTTCTC TCCTTCTGTG GTTCATCTGG GGGTCCCCCT ATCGGTGGGG 
GTGCAGCTCC AGGATGTGCC CCGAGGACAG GTAGTGAAAG GATCAGTGTT CCTGAGAAAC 
CCATCTCGTA ATAATGTCCC CTGCTCCCCA AAGGTGGACT TCACCCTTAG CTCAGAAAGA 
GACTTCGCAC TCCTCAGTCT CCAGGTGCCC TTGAAAGATG CGAAGAGCTG TGGCCTCCAT 
CAACTCCTCA GAGGCCCTGA GGTCCAGCTG GTGGCCCATT CGCCATGGCT AAAGGACTCT 
CTGTCCAGAA CGACAAACAT CCAGGGTATC AACCTGCTCT TCTCCTCTCG CCGGGGGCAC 
CTCTTTTTGC AGACGGACCA GCCCATTTAC AACCCTGGCC AGCGGGTTCG GTACCGGGTC 
TTTGCTCTGG ATCAGAAGAT GCGCCCGAGC ACTGACACCA TCACAGTCAT GGTGGAGAAC 
TCTCACGGCC TCCGCGTGCG GAAGAAGGAG GTGTACATGC CCTCGTCCAT CTTCCAGGAT 
GACTTTGTGA TCCCAGACAT CTCAGAGCCA GGGACCTGGA AGATCTCAGC CCGATTCTCA 
GATGGCCTGG AATCCAACAG CAGCACCCAG TTTGAGGTGA AGAAATATGT CCTTCCCAAC 
TTTGAGGTGA AGATCACCCC TGGAAAGCCC TACATCCTGA CGGTGCCAGG CCATCTTGAT 
GAAATGCAGT TAGACATCCA GGCCAGGTAC ATCTATGGGA AGCCAGTGCA GGGGGTGGCA 
TATGTGCGCT TTGGGCTCCT AGATGAGGAT GGTAAGAAGA CTTTCTTTCG GGGGCTGGAG 
AGTCAGACCA AGCTGGTGAA TGGACAGAGC CACATTTCCC TCTCAAAGGC AGAGTTCCAG 
GACGCCCTGG AGAAGCTGAA TATGGGCATT ACTGACCTCC AGGGGCTGCG CCTCTACGTT 
GCTGCAGCCA TCATTGAGTC TCCAGGTGGG GAGATGGAGG AGGCAGAGCT CACATCCTGG 
TATTTTGTGT CATCTCCCTT CTCCTTGGAT CTTAGCAAGA CCAAGCGACA CCTTGTGCCT 
GGGGCCCCCT TCCTGCTGCA GGCCTTGGTC CGTGAGATGT CAGGCTCCCC AGCTTCTGGC 
ATTCCTGTCA AAGTTTCTGC CACGGTGTCT TCTCCTGGGT CTGTTCCTGA AGTCCAGGAC 
ATTCAGCAAA ACACAGACGG GAGCGGCCAA GTCAGCATTC CAATAATTAT CCCTCAGACC 
ATCTCAGAGC TGCAGCTCTC AGTATCTGCA GGCTCCCCAC ATCCAGCGAT AGCCAGGCTC 
ACTGTGGCAG CCCCACCTTC AGGAGGCCCC GGGTTTCTGT CTATTGAGCG GCCGGATTCT 
CGACCTCCTC GTGTTGGGGA CACTCTGAAC CTGAACTTGC GAGCCGTGGG CAGTGGGGCC 
ACCTTTTCTC ATTACTACTA CATGATCCTA TCCCGAGGGC AGATCGTGTT CATGAATCGA 
GAGCCCAAGA GGACCCTGAC CTCGGTCTCG GTGTTTGTGG ACCATCACCT GGCACCCTCC 
TTCTACTTTG TGGCCTTCTA CTACCATGGA GACCACCCAG TGGCCAACTC CCTGCGAGTG 
GATGTCCAGG CTGGGGCCTG CGAGGGCAAG CTGGAGCTCA GCGTGGACGG TGCCAAGCAG 
TACCGGAACG GGGAGTCCGT GAAGCTCCAC TTAGAAACCG ACTCCCTAGC CCTGGTGGCG 
CTGGGAGCCT TGGACACAGC TCTGTATGCT GCAGGCAGCA AGTCCCACAA GCCCCTCAAC 
ATGGGCAAGG TCTTTGAAGC TATGAACAGC TATGACCTCG GCTGTGGTCC TGGGGGTGGG 
GACAGTGCCC TTCAGGTGTT CCAGGCAGCG GGCCTGGCCT TTTCTGATGG AGACCAGTGG 
ACCTTATCCA GAAAGAGACT AAGCTGTCCC AAGGAGAAGA CAACCCGGAA AAAGAGAAAC 
GTGAACTTCC AAAAGGCGAT TAATGAGAAA TTGGGTCAGT ATGCTTCCCC GACAGCCAAG 
CGCTGCTGCC AGGATGGGGT GACACGTCTG CCCATGATGC GTTCCTGCGA GCAGCGGGCA 
GCCCGCGTGC AGCAGCCGGA CTGCCGGGAG CCCTTCCTGT CCTGCTGCCA ATTTGCTGAG 
AGTCTGCGCA AGAAGAGCAG GGACAAGGGC CAGGCGGGCC TCCAACGAGC CCTGGAGATC 
CTGCAGGAGG AGGACCTGAT TGATGAGGAT GACATTCCCG TGCGCAGCTT CTTCCCAGAG 
AACTGGCTCT GGAGAGTGGA AACAGTGGAC CGCTTTCAAA TATTGACACT GTGGCTCCCC 
GACTCTCTGA CCACGTGGGA GATCCATGGC CTGAGCCTGT CCAAAACCAA AGGCCTATGT 
GTGGCCACCC CAGTCCAGCT CCGGGTGTTC CGCGAGTTCC ACCTGCACCT CCGCCTGCCC 
ATGTCTGTCC GCCGCTTTGA GCAGCTGGAG CTGCGGCCTG TCCTCTATAA CTACCTGGAT 
AAAAACCTGA CTGTGAGCGT CCACGTGTCC CCAGTGGAGG GGCTGTGCCT GGCTGGGGGC 
GGAGGGCTGG CCCAGCAGGT GCTGGTGCCT GCGGGCTCTG CCCGGCCTGT TGCCTTCTCT 
GTGGTGCCCA CGGCAGCCGC CGCTGTGTCT CTGAAGGTGG TGGCTCGAGG GTCCTTCGAA 
TTCCCTGTGG GAGATGCGGT GTCCAAGGTT CTGCAGATTG AGAAGGAAGG GGCCATCCAT 
AGAGAGGAGC TGGTCTATGA ACTCAACCCC TTGGACCACC GAGGCCGGAC CTTGGAAATA 
CCTGGCAACT CTGATCCCAA TATGATCCCT GATGGGGACT TTAACAGCTA CGTCAGGGTT 
ACAGCCTCAG ATCCATTGGA CACTTTAGGC TCTGAGGGGG CCTTGTCACC AGGAGGCGTG 
GCCTCCCTCT TGAGGCTTCC TCGAGGCTGT GGGGAGCAAA CCATGATCTA CTTGGCTCCG 
ACACTGGCTG CTTCCCGCTA CCTGGACAAG ACAGAGCAGT GGAGCACACT GCCTCCCGAG 
ACCAAGGACC ACGCCGTGGA TCTGATCCAG AAAGGCTACA TGCGGATCCA GCAGTTTCGG 
AAGGCGGATG GTTCCTATGC GGCTTGGTTG TCACGGGACA GCAGCACCTG GCTCACAGCC 
TTTGTGTTGA AGGTCCTGAG TTTGGCCCAG GAGCAGGTAG GAGGCTCGCC TGAGAAACTG 
CAGGAGACAT CTAACTGGCT TCTGTCCCAG CAGCAGGCTG ACGGCTCGTT CCAGGACCCC 
TGTCCAGTGT TAGACAGGAG CATGCAGGGG GGTTTGGTGG GCAATGATGA GACTGTGGCA 
CTCACAGCCT TTGTGACCAT CGCCCTTCAT CATGGGCTGG CCGTCTTCCA GGATGAGGGT 
GCAGAGCCAT TGAAGCAGAG AGTGGAAGCC TCCATCTCAA AGGCAAACTC ATTTTTGGGG 
GAGAAAGCAA GTGCTGGGCT CCTGGGTGCC CACGCAGCTG CCATCACGGC CTATGCCCTG 
ACACTGACCA AGGCGCCTGT GGACCTGCTC GGTGTTGCCC ACAACAACCT CATGGCAATG 
GCCCAGGAGA CTGGAGATAA CCTGTACTGG GGCTCAGTCA CTGGTTCTCA GAGCAATGCC 
GTGTCGCCCA CCCCGGCTCC TCGCAACCCA TCCGACCCCA TGCCCCAGGC CCCAGCCCTG 
TGGATTGAAA CCACAGCCTA CGCCCTGCTG CACCTCCTGC TTCACGAGGG CAAAGCAGAG 
ATGGCAGACC AGGCTTCGGC CTGGCTCACC CGTCAGGGCA GCTTCCAAGG GGGATTCCGC 
AGTACCCAAG ACACGGTGAT TGCCCTGGAT GCCCTGTCTG CCTACTGGAT TGCCTCCCAC 
ACCACTGAGG AGAGGGGTCT CAATGTGACT CTCAGCTCCA CAGGCCGGAA TGGGTTCAAG 
TCCCACGCGC TGCAGCTGAA CAACCGCCAG ATTCGCGGCC TGGAGGAGGA GCTGCAGTTT 
TCCTTGGGCA GCAAGATCAA TGTGAAGGTG GGAGGAAACA GCAAAGGAAC CCTGAAGGTC 
CTTCGTACCT ACAATGTCCT GGACATGAAG AACACGACCT GCCAGGACCT ACAGATAGAA 
GTGACAGTCA AAGGCCACGT CGAGTACACG ATGGAAGCAA ACGAGGACTA TGAGGACTAT 
GAGTACGATG AGCTTCCAGC CAAGGATGAC CCAGATGCCC CTCTGCAGCC CGTGACACCC 
CTGCAGCTGT TTGAGGGTCG GAGGAACCGC CGCAGGAGGG AGGCGCCCAA GCTGACCTCC 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACCGAGGGGC CCCACGTCCT GCTGTATTTT 
GACTCGGTCC CCACCTCCCG GGAGTGCGTG GGCTTTGAGG CTGTGCAGGA AGTGCCGGTG 
GGGCTGGTGC AGCCGGCCAG CGCAACCCTG TACGACTACT ACAACCCCGA GCGCAGATGT 
TCTGTGTTTT ACGGGGCACC AAGTAAGAGC AGACTCTTGG CCACCTTGTG TTCTGCTGAA 
GTCTGCCAGT GTGCTGAGGG GAAGTGCCCT CGCCAGCGTC GCGCCCTGGA GCGGGGTCTG 
CAGGACGAGG ATGGCTACAG GATGAAGTTT GCCTGCTACT ACCCCCGTGT GGAGTACGGC 
TTCCAGGTTA AGGTTCTCCG AGAAGACAGC AGAGCTGCTT TCCGCCTCTT TGAGACCAAG 
ATCACCCAAG TCCTGCACTT CACCAAGGAT GTCAAGGCCG CTGCTAATCA GATGCGCAAC 
TTCCTGGTTC GAGCCTCCTG CCGCCTTCGC TTGGAACCTG GGAAAGAATA TTTGATCATG 
GGTCTGGATG GGGCCACCTA TGACCTCGAG GGACACCCCC AGTACCTGCT GGACTCGAAT 
AGCTGGATCG AGGAGATGCC CTCTGAACGC CTGTGCCGGA GCACCCGCCA GCGGGCAGCC 
TGTGCCCAGC TCAACGACTT CCTCCAGGAG TATGGCACTC AGGGGTGCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239133.1
CDS	52..5148
Translation	MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAGLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKANSFLGEKASAGLLGAHAAAITAYALTLTKAPVDLLGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQASAWLTRQGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQIRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREDSRAAFRLFETKITQVLHFTKDVKAAANQMRNFLVRASCRLRLEPGKEYLIMGLDGATYDLEGHPQYLLDSNSWIEEMPSERLCRSTRQRAACAQLNDFLQEYGTQGCQV

Target ORF information:

RefSeq Version	NM_001252204.1
Organism	Homo sapiens(human)
Definition	Homo sapiens complement C4A (Rodgers blood group) (C4A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252204.1

ORF Insert Sequence:

ATGAGGCTGC TCTGGGGGCT GATCTGGGCA TCCAGCTTCT TCACCTTATC TCTGCAGAAG 
CCCAGGTTGC TCTTGTTCTC TCCTTCTGTG GTTCATCTGG GGGTCCCCCT ATCGGTGGGG 
GTGCAGCTCC AGGATGTGCC CCGAGGACAG GTAGTGAAAG GATCAGTGTT CCTGAGAAAC 
CCATCTCGTA ATAATGTCCC CTGCTCCCCA AAGGTGGACT TCACCCTTAG CTCAGAAAGA 
GACTTCGCAC TCCTCAGTCT CCAGGTGCCC TTGAAAGATG CGAAGAGCTG TGGCCTCCAT 
CAACTCCTCA GAGGCCCTGA GGTCCAGCTG GTGGCCCATT CGCCATGGCT AAAGGACTCT 
CTGTCCAGAA CGACAAACAT CCAGGGTATC AACCTGCTCT TCTCCTCTCG CCGGGGGCAC 
CTCTTTTTGC AGACGGACCA GCCCATTTAC AACCCTGGCC AGCGGGTTCG GTACCGGGTC 
TTTGCTCTGG ATCAGAAGAT GCGCCCGAGC ACTGACACCA TCACAGTCAT GGTGGAGAAC 
TCTCACGGCC TCCGCGTGCG GAAGAAGGAG GTGTACATGC CCTCGTCCAT CTTCCAGGAT 
GACTTTGTGA TCCCAGACAT CTCAGAGCCA GGGACCTGGA AGATCTCAGC CCGATTCTCA 
GATGGCCTGG AATCCAACAG CAGCACCCAG TTTGAGGTGA AGAAATATGT CCTTCCCAAC 
TTTGAGGTGA AGATCACCCC TGGAAAGCCC TACATCCTGA CGGTGCCAGG CCATCTTGAT 
GAAATGCAGT TAGACATCCA GGCCAGGTAC ATCTATGGGA AGCCAGTGCA GGGGGTGGCA 
TATGTGCGCT TTGGGCTCCT AGATGAGGAT GGTAAGAAGA CTTTCTTTCG GGGGCTGGAG 
AGTCAGACCA AGCTGGTGAA TGGACAGAGC CACATTTCCC TCTCAAAGGC AGAGTTCCAG 
GACGCCCTGG AGAAGCTGAA TATGGGCATT ACTGACCTCC AGGGGCTGCG CCTCTACGTT 
GCTGCAGCCA TCATTGAGTC TCCAGGTGGG GAGATGGAGG AGGCAGAGCT CACATCCTGG 
TATTTTGTGT CATCTCCCTT CTCCTTGGAT CTTAGCAAGA CCAAGCGACA CCTTGTGCCT 
GGGGCCCCCT TCCTGCTGCA GGCCTTGGTC CGTGAGATGT CAGGCTCCCC AGCTTCTGGC 
ATTCCTGTCA AAGTTTCTGC CACGGTGTCT TCTCCTGGGT CTGTTCCTGA AGTCCAGGAC 
ATTCAGCAAA ACACAGACGG GAGCGGCCAA GTCAGCATTC CAATAATTAT CCCTCAGACC 
ATCTCAGAGC TGCAGCTCTC AGTATCTGCA GGCTCCCCAC ATCCAGCGAT AGCCAGGCTC 
ACTGTGGCAG CCCCACCTTC AGGAGGCCCC GGGTTTCTGT CTATTGAGCG GCCGGATTCT 
CGACCTCCTC GTGTTGGGGA CACTCTGAAC CTGAACTTGC GAGCCGTGGG CAGTGGGGCC 
ACCTTTTCTC ATTACTACTA CATGATCCTA TCCCGAGGGC AGATCGTGTT CATGAATCGA 
GAGCCCAAGA GGACCCTGAC CTCGGTCTCG GTGTTTGTGG ACCATCACCT GGCACCCTCC 
TTCTACTTTG TGGCCTTCTA CTACCATGGA GACCACCCAG TGGCCAACTC CCTGCGAGTG 
GATGTCCAGG CTGGGGCCTG CGAGGGCAAG CTGGAGCTCA GCGTGGACGG TGCCAAGCAG 
TACCGGAACG GGGAGTCCGT GAAGCTCCAC TTAGAAACCG ACTCCCTAGC CCTGGTGGCG 
CTGGGAGCCT TGGACACAGC TCTGTATGCT GCAGGCAGCA AGTCCCACAA GCCCCTCAAC 
ATGGGCAAGG TCTTTGAAGC TATGAACAGC TATGACCTCG GCTGTGGTCC TGGGGGTGGG 
GACAGTGCCC TTCAGGTGTT CCAGGCAGCG GGCCTGGCCT TTTCTGATGG AGACCAGTGG 
ACCTTATCCA GAAAGAGACT AAGCTGTCCC AAGGAGAAGA CAACCCGGAA AAAGAGAAAC 
GTGAACTTCC AAAAGGCGAT TAATGAGAAA TTGGGTCAGT ATGCTTCCCC GACAGCCAAG 
CGCTGCTGCC AGGATGGGGT GACACGTCTG CCCATGATGC GTTCCTGCGA GCAGCGGGCA 
GCCCGCGTGC AGCAGCCGGA CTGCCGGGAG CCCTTCCTGT CCTGCTGCCA ATTTGCTGAG 
AGTCTGCGCA AGAAGAGCAG GGACAAGGGC CAGGCGGGCC TCCAACGAGC CCTGGAGATC 
CTGCAGGAGG AGGACCTGAT TGATGAGGAT GACATTCCCG TGCGCAGCTT CTTCCCAGAG 
AACTGGCTCT GGAGAGTGGA AACAGTGGAC CGCTTTCAAA TATTGACACT GTGGCTCCCC 
GACTCTCTGA CCACGTGGGA GATCCATGGC CTGAGCCTGT CCAAAACCAA AGGCCTATGT 
GTGGCCACCC CAGTCCAGCT CCGGGTGTTC CGCGAGTTCC ACCTGCACCT CCGCCTGCCC 
ATGTCTGTCC GCCGCTTTGA GCAGCTGGAG CTGCGGCCTG TCCTCTATAA CTACCTGGAT 
AAAAACCTGA CTGTGAGCGT CCACGTGTCC CCAGTGGAGG GGCTGTGCCT GGCTGGGGGC 
GGAGGGCTGG CCCAGCAGGT GCTGGTGCCT GCGGGCTCTG CCCGGCCTGT TGCCTTCTCT 
GTGGTGCCCA CGGCAGCCGC CGCTGTGTCT CTGAAGGTGG TGGCTCGAGG GTCCTTCGAA 
TTCCCTGTGG GAGATGCGGT GTCCAAGGTT CTGCAGATTG AGAAGGAAGG GGCCATCCAT 
AGAGAGGAGC TGGTCTATGA ACTCAACCCC TTGGACCACC GAGGCCGGAC CTTGGAAATA 
CCTGGCAACT CTGATCCCAA TATGATCCCT GATGGGGACT TTAACAGCTA CGTCAGGGTT 
ACAGCCTCAG ATCCATTGGA CACTTTAGGC TCTGAGGGGG CCTTGTCACC AGGAGGCGTG 
GCCTCCCTCT TGAGGCTTCC TCGAGGCTGT GGGGAGCAAA CCATGATCTA CTTGGCTCCG 
ACACTGGCTG CTTCCCGCTA CCTGGACAAG ACAGAGCAGT GGAGCACACT GCCTCCCGAG 
ACCAAGGACC ACGCCGTGGA TCTGATCCAG AAAGGCTACA TGCGGATCCA GCAGTTTCGG 
AAGGCGGATG GTTCCTATGC GGCTTGGTTG TCACGGGACA GCAGCACCTG GCTCACAGCC 
TTTGTGTTGA AGGTCCTGAG TTTGGCCCAG GAGCAGGTAG GAGGCTCGCC TGAGAAACTG 
CAGGAGACAT CTAACTGGCT TCTGTCCCAG CAGCAGGCTG ACGGCTCGTT CCAGGACCCC 
TGTCCAGTGT TAGACAGGAG CATGCAGGGG GGTTTGGTGG GCAATGATGA GACTGTGGCA 
CTCACAGCCT TTGTGACCAT CGCCCTTCAT CATGGGCTGG CCGTCTTCCA GGATGAGGGT 
GCAGAGCCAT TGAAGCAGAG AGTGGAAGCC TCCATCTCAA AGGCAAACTC ATTTTTGGGG 
GAGAAAGCAA GTGCTGGGCT CCTGGGTGCC CACGCAGCTG CCATCACGGC CTATGCCCTG 
ACACTGACCA AGGCGCCTGT GGACCTGCTC GGTGTTGCCC ACAACAACCT CATGGCAATG 
GCCCAGGAGA CTGGAGATAA CCTGTACTGG GGCTCAGTCA CTGGTTCTCA GAGCAATGCC 
GTGTCGCCCA CCCCGGCTCC TCGCAACCCA TCCGACCCCA TGCCCCAGGC CCCAGCCCTG 
TGGATTGAAA CCACAGCCTA CGCCCTGCTG CACCTCCTGC TTCACGAGGG CAAAGCAGAG 
ATGGCAGACC AGGCTTCGGC CTGGCTCACC CGTCAGGGCA GCTTCCAAGG GGGATTCCGC 
AGTACCCAAG ACACGGTGAT TGCCCTGGAT GCCCTGTCTG CCTACTGGAT TGCCTCCCAC 
ACCACTGAGG AGAGGGGTCT CAATGTGACT CTCAGCTCCA CAGGCCGGAA TGGGTTCAAG 
TCCCACGCGC TGCAGCTGAA CAACCGCCAG ATTCGCGGCC TGGAGGAGGA GCTGCAGTTT 
TCCTTGGGCA GCAAGATCAA TGTGAAGGTG GGAGGAAACA GCAAAGGAAC CCTGAAGGTC 
CTTCGTACCT ACAATGTCCT GGACATGAAG AACACGACCT GCCAGGACCT ACAGATAGAA 
GTGACAGTCA AAGGCCACGT CGAGTACACG ATGGAAGCAA ACGAGGACTA TGAGGACTAT 
GAGTACGATG AGCTTCCAGC CAAGGATGAC CCAGATGCCC CTCTGCAGCC CGTGACACCC 
CTGCAGCTGT TTGAGGGTCG GAGGAACCGC CGCAGGAGGG AGGCGCCCAA GCTGACCTCC 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACCGAGGGGC CCCACGTCCT GCTGTATTTT 
GACTCGGTCC CCACCTCCCG GGAGTGCGTG GGCTTTGAGG CTGTGCAGGA AGTGCCGGTG 
GGGCTGGTGC AGCCGGCCAG CGCAACCCTG TACGACTACT ACAACCCCGA GCGCAGATGT 
TCTGTGTTTT ACGGGGCACC AAGTAAGAGC AGACTCTTGG CCACCTTGTG TTCTGCTGAA 
GTCTGCCAGT GTGCTGAGGG GAAGTGCCCT CGCCAGCGTC GCGCCCTGGA GCGGGGTCTG 
CAGGACGAGG ATGGCTACAG GATGAAGTTT GCCTGCTACT ACCCCCGTGT GGAGTACGGC 
TTCCAGGTTA AGGTTCTCCG AGAAGACAGC AGAGCTGCTT TCCGCCTCTT TGAGACCAAG 
ATCACCCAAG TCCTGCACTT CACCAAGGAT GTCAAGGCCG CTGCTAATCA GATGCGCAAC 
TTCCTGGTTC GAGCCTCCTG CCGCCTTCGC TTGGAACCTG GGAAAGAATA TTTGATCATG 
GGTCTGGATG GGGCCACCTA TGACCTCGAG GGACACCCCC AGTACCTGCT GGACTCGAAT 
AGCTGGATCG AGGAGATGCC CTCTGAACGC CTGTGCCGGA GCACCCGCCA GCGGGCAGCC 
TGTGCCCAGC TCAACGACTT CCTCCAGGAG TATGGCACTC AGGGGTGCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Preservation of antimicrobial properties of complement peptide C3a, from invertebrates to humans.
The Journal of biological chemistry282(4)2520-8(2007 Jan)
Pasupuleti M,Walse B,Nordahl EA,M?rgelin M,Malmsten M,Schmidtchen A

The structural basis of the multiple forms of human complement component C4.
Cell36(4)907-14(1984 Apr)
Belt KT,Carroll MC,Porter RR

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C4A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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