C4b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	C4b
Entrez Gene ID	12268
Full Name	complement component 4B (Chido blood group)
Synonyms	C4,Ss
General protein information	Preferred Names complement component 4B (Chido blood group) Names complement C4-B complement component 4 (within H-2S) complement component 4B (Childo blood group)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B1\|17 18.29 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006523531.3	XP_006523594.1	complement C4-B isoform X1
XM_006523532.4	XP_006523595.1	complement C4-B isoform X2
XM_006523531.4	XP_006523594.1	complement C4-B isoform X1
XM_006523532.3	XP_006523595.1	complement C4-B isoform X2
XM_030249459.1	XP_030105319.1	complement C4-B isoform X3
NM_009780.2	NP_033910.2	complement C4-B precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	C4a	NP_035543.2
Danio rerio (zebrafish)	si:dkey-8k3.2	XP_001334640.1
Mus musculus (house mouse)	C4b	NP_033910.2
Rattus norvegicus (Norway rat)	C4b	NP_001002805.1
Homo sapiens (human)	C4A	NP_009224.2
Pan troglodytes (chimpanzee)	C4A	XP_003311220.1
Canis lupus familiaris (dog)	C4A	XP_005627805.1
Bos taurus (cattle)	C4A	NP_001159957.1
Gallus gallus (chicken)	C4	NP_001070701.1
Homo sapiens (human)	C4B	NP_001002029.3
Xenopus tropicalis (tropical clawed frog)	c4a	NP_001107156.1
Rattus norvegicus (Norway rat)	C4a	NP_113692.2

Gene Ontology
Bibliography

Related articles in PubMed

Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival.
Caine JA, Lin YP, Kessler JR, Sato H, Leong JM, Coburn J
Cellular microbiology19(12)(2017 Dec)

Synergy between the classical and alternative pathways of complement is essential for conferring effective protection against the pandemic influenza A(H1N1) 2009 virus infection.
Rattan A, Pawar SD, Nawadkar R, Kulkarni N, Lal G, Mullick J, Sahu A
PLoS pathogens13(3)e1006248(2017 Mar)

Collectin-11 detects stress-induced L-fucose pattern to trigger renal epithelial injury.
Farrar CA, Tran D, Li K, Wu W, Peng Q, Schwaeble W, Zhou W, Sacks SH
The Journal of clinical investigation126(5)1911-25(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

C4 mediated synapse elimination during postnatal development
Title: Schizophrenia risk from complex variation of complement component 4.

role in suppressing autoantibody production elicited by S. pneumoniae antigens
Title: C4 Deficiency is a predisposing factor for Streptococcus pneumoniae-induced autoantibody production.

Data indicate complement C3 deposition on peanut extract (PE) was abolished in immunoglobulin- and C4-deficient sera.
Title: Role of complement in a murine model of peanut-induced anaphylaxis.

a deficiency in C4b does not alter radiation-induced lung disease in the C57BL/6 mouse model
Title: Genetic deficiency in complement component 4b does not alter radiation-induced lung disease in mice.

C3 and C4 bind to collagen and elastin in the vascular wall and have a potential role in vascular stiffness and atherosclerosis
Title: Complement proteins C3 and C4 bind to collagen and elastin in the vascular wall: a potential role in vascular stiffness and atherosclerosis.

The following C4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the C4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22129	NM_009780.2 Latest version!	Mus musculus complement component 4B (Chido blood group) (C4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1280.30 $1829.00
OMu53047	XM_006523531.4 Latest version!	Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu53048	XM_006523532.4 Latest version!	Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu87079	XM_030249459.1 Latest version!	Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OMu53047	XM_006523531.3	Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu53048	XM_006523532.3	Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

C4b ( NM_009780.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033910.2 Mus musculus complement component 4B (Chido blood group) (C4b), mRNA.

Price & Availability↑

CloneID	OMu22129
Clone ID Related Accession (Same CDS sequence)	NM_009780.2
Accession Version	NM_009780.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	complement C4-B precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY785731.1, BC067394.1, CA459454.1, AK158475.1, AK158256.1, AK128974.1, K02798.1 and AK157954.1. On Oct 6, 2007 this sequence version replaced NM_009780.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC067394.1, AK157954.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCTCC TCTGGGGGCT GGCCTGGGTG TTCAGCTTCT GTGCCTCATC CCTGCAGAAG 
CCCAGGTTGC TCCTGTTTTC CCCTTCTGTG GTTAATTTGG GGACCCCCCT GTCGGTGGGG 
GTACAGCTCC TGGATGCCCC TCCAGGACAG GAGGTAAAAG GATCAGTGTT CCTCAGAAAC 
CCAAAGGGTG GTTCCTGCTC CCCAAAGAAG GACTTTAAGC TGAGCTCGGG AGATGACTTT 
GTGCTGCTCA GCCTTGAGGT CCCACTGGAA GATGTGAGGA GCTGTGGCCT CTTTGACCTG 
CGCAGAGCCC CCCACATCCA GCTGGTAGCT CAGTCTCCGT GGCTAAGGAA CACAGCTTTC 
AAAGCCACAG AGACTCAGGG TGTCAACTTG CTCTTCTCTT CCCGACGAGG CCACATCTTT 
GTGCAGACCG ATCAGCCTAT CTATAATCCG GGGCAGCGGG TTCGTTATCG GGTCTTTGCA 
CTGGATCAAA AGATGCGCCC ATCCACTGAT TTCCTCACCA TCACAGTGGA GAACTCCCAT 
GGCCTCCGTG TACTCAAGAA GGAGATATTT ACTTCCACAT CCATCTTCCA AGATGCCTTC 
ACCATTCCAG ACATCTCAGA GCCTGGGACC TGGAAGATCT CAGCTAGGTT CTCAGATGGA 
CTGGAGTCCA ATAGGAGCAC CCACTTTGAA GTGAAGAAGT ATGTCCTTCC CAACTTCGAG 
GTGAAGATTA CTCCTTGGAA GCCATATATC CTGATGGTGC CCAGCAACAG TGATGAAATC 
CAATTAGACA TCCAGGCCAG GTACATCTAT GGGAAGCCCG TGCAGGGCGT GGCATACACA 
CGGTTTGCGC TCATGGATGA GCAAGGGAAG AGGACTTTCC TTCGGGGCCT AGAGACGCAG 
GCCAAGTTGG TGGAAGGCCG GACCCACATT TCCATCTCAA AGGACCAGTT CCAGGCTGCC 
CTGGATAAAA TCAATATTGG GGTCAGAGAC CTGGAGGGGC TGCGTCTCTA TGCTGCTACA 
GCTGTCATCG AGTCTCCAGG AGGAGAGATG GAGGAGGCAG AACTCACGTC CTGGCGCTTT 
GTATCATCTG CCTTCTCCTT GGATCTCAGC CGCACTAAGA GGCATCTTGT GCCTGGGGCC 
CACTTCCTGC TGCAGGCTCT GGTCCAAGAA ATGTCAGGCT CTGAAGCCTC CAACGTTCCT 
GTTAAAGTCT CTGCCACATT GGTGTCAGGC TCTGACTCCC AAGTCCTTGA CATTCAACAG 
AGCACCAATG GAATTGGCCA AGTCAGCATT TCCTTCCCCA TCCCACCAAC TGTCACAGAA 
CTTCGACTCT TGGTGTCTGC GGGCTCCCTC TACCCAGCCA TAGCCAGGCT CACCGTGCAA 
GCCCCACCTT CAAGAGGCAC TGGCTTTCTT TCTATTGAGC CACTAGACCC TCGGTCCCCT 
AGTGTGGGGG ACACCTTTAT CCTAAACCTT CAACCTGTGG GCATCCCTGC ACCTACCTTC 
TCTCATTACT ACTACATGAT CATCTCCAGA GGCCAGATCA TGGCTATGGG TCGGGAGCCC 
CGGAAGACTG TGACCTCCGT CTCTGTGTTG GTGGACCATC AGCTGGCTCC CTCGTTCTAC 
TTTGTGGCTT ACTTCTATCA CCAAGGACAC CCGGTGGCCA ACTCTCTGCT CATCAACATC 
CAATCCAGGG ACTGTGAGGG CAAGCTGCAA TTGAAGGTGG ATGGTGCCAA GGAGTATCGT 
AATGCGGACA TGATGAAGCT CCGAATTCAA ACTGACTCCA AAGCCCTGGT GGCACTGGGA 
GCTGTGGACA TGGCTCTGTA TGCTGTGGGT GGTCGGTCTC ACAAACCCCT CGACATGAGC 
AAGGTCTTTG AAGTAATCAA CAGCTACAAT GTTGGCTGTG GTCCTGGAGG TGGGGATGAT 
GCCCTTCAGG TGTTCCAGGA TGCTGGTCTG GCCTTTTCTG ATGGTGATCG ACTAACTCAA 
ACCAGAGAGG ACCTGAGCTG TCCCAAGGAG AAGAAAAGTC GGCAAAAGAG AAATGTTAAC 
TTCCAGAAGG CTGTCAGTGA GAAGTTGGGC CAGTATTCTT CTCCAGATGC CAAGCGCTGC 
TGCCAAGACG GGATGACGAA GCTGCCCATG AAGCGTACCT GTGAGCAGCG GGCTGCCCGT 
GTGCCTCAGC AGGCCTGCCG TGAGCCCTTC TTGTCCTGTT GCAAGTTTGC TGAGGACCTT 
CGCAGGAACC AGACCAGGAG CCAGGCACAC CTTGCCCGAA ACAACCACAA CATGCTGCAG 
GAGGAAGACT TGATAGATGA AGACGACATT CTTGTGCGCA CCTCCTTCCC AGAGAACTGG 
CTCTGGAGAG TGGAACCTGT AGACAGCTCC AAACTGTTGA CAGTGTGGCT TCCTGATTCT 
ATGACCACAT GGGAGATTCA TGGTGTGAGC CTGTCCAAAA GCAAAGGTCT GTGTGTAGCC 
AAGCCAACTC GTGTTCGAGT GTTCAGAAAA TTCCACCTGC ACCTGCGCCT GCCCATCTCC 
ATCCGCCGCT TTGAGCAGTT TGAATTACGG CCTGTTCTTT ACAACTATCT GAATGATGAT 
GTGGCTGTGA GTGTCCATGT GACCCCAGTT GAGGGGCTGT GCCTGGCTGG TGGTGGAATG 
ATGGCCCAGC AGGTGACAGT GCCAGCAGGT TCTGCCCGGC CTGTGGCCTT CTCTGTGGTA 
CCCACAGCTG CTGCCAACGT GCCCCTGAAG GTGGTGGCTC GAGGGGTTTT TGATTTAGGG 
GATGCTGTGT CTAAGATTCT CCAAATTGAG AAGGAAGGAG CCATCCACAG AGAAGAGTTA 
GTCTACAACC TCGACCCCCT AAATAACCTG GGTCGGACTT TGGAGATTCC TGGCAGCTCG 
GATCCCAACA TCGTCCCTGA CGGAGACTTC AGCAGCTTAG TCAGGGTTAC AGCCTCGGAA 
CCCTTGGAGA CTATGGGTTC TGAAGGTGCC TTGTCCCCAG GAGGTGTGGC CTCCCTCCTG 
AGGCTTCCCC AGGGCTGTGC AGAGCAAACC ATGATCTATT TGGCTCCTAC CCTGACTGCT 
TCCAACTACC TGGACAGGAC AGAACAGTGG AGCAAACTGT CCCCTGAAAC AAAGGACCAT 
GCTGTGGATC TGATCCAAAA AGGATACATG AGGATCCAGC AGTTTCGGAA GAATGATGGC 
TCCTTTGGGG CTTGGTTACA CCGGGACAGC AGCACCTGGC TGACTGCCTT CGTGCTGAAG 
ATTCTGAGTT TGGCCCAGGA ACAGGTGGGC AACTCCCCGG AGAAGCTGCA GGAGACGGCT 
AGCTGGCTGC TGGCCCAGCA GCTGGGTGAT GGCTCCTTCC ACGACCCATG TCCAGTCATC 
CACAGAGCAA TGCAGGGGGG CTTGGTGGGG TCTGACGAGA CAGTGGCACT GACCGCCTTT 
GTGGTCATTG CCCTTCACCA TGGGTTGGAC GTCTTCCAGG ATGACGATGC GAAGCAGCTG 
AAGAACAGAG TGGAAGCCTC CATCACCAAG GCAAACTCAT TCTTGGGGCA GAAGGCAAGT 
GCTGGGCTCC TGGGTGCCCA TGCCGCCGCC ATCACAGCCT ATGCCCTTAC GCTGACCAAG 
GCCTCGGAGG ACCTGCGGAA TGTTGCCCAC AACAGCCTGA TGGCCATGGC TGAGGAAACA 
GGGGAACACC TCTACTGGGG CTTAGTCCTT GGCTCTCAGG ACAAAGTTGT GTTGCGCCCC 
ACAGCCCCCC GTAGCCCTAC AGAACCTGTG CCCCAGGCCC CAGCCTTGTG GATCGAAACC 
ACAGCCTATG CCCTGCTCCA CCTGCTTCTG CGGGAGGGAA AGGGAAAAAT GGCTGACAAG 
GCTGCATCCT GGCTCACCCA CCAGGGAAGC TTCCATGGGG CATTCCGCAG TACCCAGGAC 
ACTGTGGTCA CCCTGGATGC CCTGTCTGCC TACTGGATCG CTTCGCACAC CACTGAGGAG 
AAAGCACTGA ACGTGACGCT CAGCTCCATG GGCCGCAATG GGCTTAAGAC CCACGGGCTA 
CACTTGAACA ACCACCAAGT CAAGGGCCTG GAGGAGGAGC TAAAGTTCTC CCTGGGCAGC 
ACAATCAGTG TCAAGGTGGA AGGAAACAGC AAAGGCACCT TGAAGATCCT TCGTACCTAC 
AATGTCCTGG ACATGAAGAA CACCACATGC CAGGACCTTC AGATAGAAGT GAAGGTCACA 
GGCGCTGTGG AATACGCATG GGATGCCAAT GAAGACTACG AAGACTACTA TGACATGCCA 
GCCGCAGATG ACCCCAGCGT CCCCTTGCAG CCTGTCACGC CCCTGCAGCT TTTTGAGGGT 
CGTAGGAGCC GCCGCAGGAG GGAGGCCCCC AAGGTGGTCG AAGAGCAGGA GTCCAGAGTT 
CAGTACACTG TGTGTATCTG GCGAAATGGC AAGCTGGGGC TGTCTGGCAT GGCCATCGCA 
GACATCACCC TCCTAAGTGG ATTCCACGCC CTGAGGGCTG ACCTGGAGAA GCTGACCTCT 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACTGACGGGC CCCATGTCCT GTTGTACTTT 
GACTCGGTCC CTACCACCCG GGAGTGTGTG GGCTTCGGAG CCTCGCAGGA GGTGGTTGTG 
GGACTGGTGC AGCCATCCAG TGCTGTCCTG TATGACTACT ACAGCCCTGA TCACAAGTGC 
TCTGTGTTTT ATGCTGCACC CACCAAGAGC CAGCTCCTGG CCACACTGTG CTCTGGAGAT 
GTATGCCAGT GCGCTGAGGG GAAGTGCCCT CGACTGCTAA GGTCACTGGA GCGAAGGGTG 
GAGGACAAGG ACGGCTACCG GATGAGGTTC GCCTGCTATT ATCCCCGAGT GGAGTATGGC 
TTCACGGTTA AGGTTCTTCG AGAAGATGGC AGAGCTGCCT TCCGTCTCTT TGAGTCCAAG 
ATCACCCAAG TCCTGCATTT CAGAAAGGAC ACCATGGCCT CCATAGGTCA GACCCGCAAC 
TTCCTGAGCC GGGCCTCTTG CCGCCTTCGT TTGGAGCCTA ACAAAGAGTA CTTGATCATG 
GGGATGGACG GGGAAACCAG TGACAACAAG GGAGACCCCC AGTACTTGCT GGACTCAAAT 
ACCTGGATTG AGGAGATGCC TTCAGAACAA ATGTGCAAGA GCACCCGCCA TCGGGCAGCC 
TGTTTCCAGC TCAAAGACTT CCTGATGGAG TTCAGCAGCC GGGGGTGCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033910.2
CDS	72..5288
Translation	MRLLWGLAWVFSFCASSLQKPRLLLFSPSVVNLGTPLSVGVQLLDAPPGQEVKGSVFLRNPKGGSCSPKKDFKLSSGDDFVLLSLEVPLEDVRSCGLFDLRRAPHIQLVAQSPWLRNTAFKATETQGVNLLFSSRRGHIFVQTDQPIYNPGQRVRYRVFALDQKMRPSTDFLTITVENSHGLRVLKKEIFTSTSIFQDAFTIPDISEPGTWKISARFSDGLESNRSTHFEVKKYVLPNFEVKITPWKPYILMVPSNSDEIQLDIQARYIYGKPVQGVAYTRFALMDEQGKRTFLRGLETQAKLVEGRTHISISKDQFQAALDKINIGVRDLEGLRLYAATAVIESPGGEMEEAELTSWRFVSSAFSLDLSRTKRHLVPGAHFLLQALVQEMSGSEASNVPVKVSATLVSGSDSQVLDIQQSTNGIGQVSISFPIPPTVTELRLLVSAGSLYPAIARLTVQAPPSRGTGFLSIEPLDPRSPSVGDTFILNLQPVGIPAPTFSHYYYMIISRGQIMAMGREPRKTVTSVSVLVDHQLAPSFYFVAYFYHQGHPVANSLLINIQSRDCEGKLQLKVDGAKEYRNADMMKLRIQTDSKALVALGAVDMALYAVGGRSHKPLDMSKVFEVINSYNVGCGPGGGDDALQVFQDAGLAFSDGDRLTQTREDLSCPKEKKSRQKRNVNFQKAVSEKLGQYSSPDAKRCCQDGMTKLPMKRTCEQRAARVPQQACREPFLSCCKFAEDLRRNQTRSQAHLARNNHNMLQEEDLIDEDDILVRTSFPENWLWRVEPVDSSKLLTVWLPDSMTTWEIHGVSLSKSKGLCVAKPTRVRVFRKFHLHLRLPISIRRFEQFELRPVLYNYLNDDVAVSVHVTPVEGLCLAGGGMMAQQVTVPAGSARPVAFSVVPTAAANVPLKVVARGVFDLGDAVSKILQIEKEGAIHREELVYNLDPLNNLGRTLEIPGSSDPNIVPDGDFSSLVRVTASEPLETMGSEGALSPGGVASLLRLPQGCAEQTMIYLAPTLTASNYLDRTEQWSKLSPETKDHAVDLIQKGYMRIQQFRKNDGSFGAWLHRDSSTWLTAFVLKILSLAQEQVGNSPEKLQETASWLLAQQLGDGSFHDPCPVIHRAMQGGLVGSDETVALTAFVVIALHHGLDVFQDDDAKQLKNRVEASITKANSFLGQKASAGLLGAHAAAITAYALTLTKASEDLRNVAHNSLMAMAEETGEHLYWGLVLGSQDKVVLRPTAPRSPTEPVPQAPALWIETTAYALLHLLLREGKGKMADKAASWLTHQGSFHGAFRSTQDTVVTLDALSAYWIASHTTEEKALNVTLSSMGRNGLKTHGLHLNNHQVKGLEEELKFSLGSTISVKVEGNSKGTLKILRTYNVLDMKNTTCQDLQIEVKVTGAVEYAWDANEDYEDYYDMPAADDPSVPLQPVTPLQLFEGRRSRRRREAPKVVEEQESRVQYTVCIWRNGKLGLSGMAIADITLLSGFHALRADLEKLTSLSDRYVSHFETDGPHVLLYFDSVPTTRECVGFGASQEVVVGLVQPSSAVLYDYYSPDHKCSVFYAAPTKSQLLATLCSGDVCQCAEGKCPRLLRSLERRVEDKDGYRMRFACYYPRVEYGFTVKVLREDGRAAFRLFESKITQVLHFRKDTMASIGQTRNFLSRASCRLRLEPNKEYLIMGMDGETSDNKGDPQYLLDSNTWIEEMPSEQMCKSTRHRAACFQLKDFLMEFSSRGCQV

Target ORF information:

RefSeq Version	NM_009780.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component 4B (Chido blood group) (C4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009780.2

ORF Insert Sequence:

ATGCGGCTCC TCTGGGGGCT GGCCTGGGTG TTCAGCTTCT GTGCCTCATC CCTGCAGAAG 
CCCAGGTTGC TCCTGTTTTC CCCTTCTGTG GTTAATTTGG GGACCCCCCT GTCGGTGGGG 
GTACAGCTCC TGGATGCCCC TCCAGGACAG GAGGTAAAAG GATCAGTGTT CCTCAGAAAC 
CCAAAGGGTG GTTCCTGCTC CCCAAAGAAG GACTTTAAGC TGAGCTCGGG AGATGACTTT 
GTGCTGCTCA GCCTTGAGGT CCCACTGGAA GATGTGAGGA GCTGTGGCCT CTTTGACCTG 
CGCAGAGCCC CCCACATCCA GCTGGTAGCT CAGTCTCCGT GGCTAAGGAA CACAGCTTTC 
AAAGCCACAG AGACTCAGGG TGTCAACTTG CTCTTCTCTT CCCGACGAGG CCACATCTTT 
GTGCAGACCG ATCAGCCTAT CTATAATCCG GGGCAGCGGG TTCGTTATCG GGTCTTTGCA 
CTGGATCAAA AGATGCGCCC ATCCACTGAT TTCCTCACCA TCACAGTGGA GAACTCCCAT 
GGCCTCCGTG TACTCAAGAA GGAGATATTT ACTTCCACAT CCATCTTCCA AGATGCCTTC 
ACCATTCCAG ACATCTCAGA GCCTGGGACC TGGAAGATCT CAGCTAGGTT CTCAGATGGA 
CTGGAGTCCA ATAGGAGCAC CCACTTTGAA GTGAAGAAGT ATGTCCTTCC CAACTTCGAG 
GTGAAGATTA CTCCTTGGAA GCCATATATC CTGATGGTGC CCAGCAACAG TGATGAAATC 
CAATTAGACA TCCAGGCCAG GTACATCTAT GGGAAGCCCG TGCAGGGCGT GGCATACACA 
CGGTTTGCGC TCATGGATGA GCAAGGGAAG AGGACTTTCC TTCGGGGCCT AGAGACGCAG 
GCCAAGTTGG TGGAAGGCCG GACCCACATT TCCATCTCAA AGGACCAGTT CCAGGCTGCC 
CTGGATAAAA TCAATATTGG GGTCAGAGAC CTGGAGGGGC TGCGTCTCTA TGCTGCTACA 
GCTGTCATCG AGTCTCCAGG AGGAGAGATG GAGGAGGCAG AACTCACGTC CTGGCGCTTT 
GTATCATCTG CCTTCTCCTT GGATCTCAGC CGCACTAAGA GGCATCTTGT GCCTGGGGCC 
CACTTCCTGC TGCAGGCTCT GGTCCAAGAA ATGTCAGGCT CTGAAGCCTC CAACGTTCCT 
GTTAAAGTCT CTGCCACATT GGTGTCAGGC TCTGACTCCC AAGTCCTTGA CATTCAACAG 
AGCACCAATG GAATTGGCCA AGTCAGCATT TCCTTCCCCA TCCCACCAAC TGTCACAGAA 
CTTCGACTCT TGGTGTCTGC GGGCTCCCTC TACCCAGCCA TAGCCAGGCT CACCGTGCAA 
GCCCCACCTT CAAGAGGCAC TGGCTTTCTT TCTATTGAGC CACTAGACCC TCGGTCCCCT 
AGTGTGGGGG ACACCTTTAT CCTAAACCTT CAACCTGTGG GCATCCCTGC ACCTACCTTC 
TCTCATTACT ACTACATGAT CATCTCCAGA GGCCAGATCA TGGCTATGGG TCGGGAGCCC 
CGGAAGACTG TGACCTCCGT CTCTGTGTTG GTGGACCATC AGCTGGCTCC CTCGTTCTAC 
TTTGTGGCTT ACTTCTATCA CCAAGGACAC CCGGTGGCCA ACTCTCTGCT CATCAACATC 
CAATCCAGGG ACTGTGAGGG CAAGCTGCAA TTGAAGGTGG ATGGTGCCAA GGAGTATCGT 
AATGCGGACA TGATGAAGCT CCGAATTCAA ACTGACTCCA AAGCCCTGGT GGCACTGGGA 
GCTGTGGACA TGGCTCTGTA TGCTGTGGGT GGTCGGTCTC ACAAACCCCT CGACATGAGC 
AAGGTCTTTG AAGTAATCAA CAGCTACAAT GTTGGCTGTG GTCCTGGAGG TGGGGATGAT 
GCCCTTCAGG TGTTCCAGGA TGCTGGTCTG GCCTTTTCTG ATGGTGATCG ACTAACTCAA 
ACCAGAGAGG ACCTGAGCTG TCCCAAGGAG AAGAAAAGTC GGCAAAAGAG AAATGTTAAC 
TTCCAGAAGG CTGTCAGTGA GAAGTTGGGC CAGTATTCTT CTCCAGATGC CAAGCGCTGC 
TGCCAAGACG GGATGACGAA GCTGCCCATG AAGCGTACCT GTGAGCAGCG GGCTGCCCGT 
GTGCCTCAGC AGGCCTGCCG TGAGCCCTTC TTGTCCTGTT GCAAGTTTGC TGAGGACCTT 
CGCAGGAACC AGACCAGGAG CCAGGCACAC CTTGCCCGAA ACAACCACAA CATGCTGCAG 
GAGGAAGACT TGATAGATGA AGACGACATT CTTGTGCGCA CCTCCTTCCC AGAGAACTGG 
CTCTGGAGAG TGGAACCTGT AGACAGCTCC AAACTGTTGA CAGTGTGGCT TCCTGATTCT 
ATGACCACAT GGGAGATTCA TGGTGTGAGC CTGTCCAAAA GCAAAGGTCT GTGTGTAGCC 
AAGCCAACTC GTGTTCGAGT GTTCAGAAAA TTCCACCTGC ACCTGCGCCT GCCCATCTCC 
ATCCGCCGCT TTGAGCAGTT TGAATTACGG CCTGTTCTTT ACAACTATCT GAATGATGAT 
GTGGCTGTGA GTGTCCATGT GACCCCAGTT GAGGGGCTGT GCCTGGCTGG TGGTGGAATG 
ATGGCCCAGC AGGTGACAGT GCCAGCAGGT TCTGCCCGGC CTGTGGCCTT CTCTGTGGTA 
CCCACAGCTG CTGCCAACGT GCCCCTGAAG GTGGTGGCTC GAGGGGTTTT TGATTTAGGG 
GATGCTGTGT CTAAGATTCT CCAAATTGAG AAGGAAGGAG CCATCCACAG AGAAGAGTTA 
GTCTACAACC TCGACCCCCT AAATAACCTG GGTCGGACTT TGGAGATTCC TGGCAGCTCG 
GATCCCAACA TCGTCCCTGA CGGAGACTTC AGCAGCTTAG TCAGGGTTAC AGCCTCGGAA 
CCCTTGGAGA CTATGGGTTC TGAAGGTGCC TTGTCCCCAG GAGGTGTGGC CTCCCTCCTG 
AGGCTTCCCC AGGGCTGTGC AGAGCAAACC ATGATCTATT TGGCTCCTAC CCTGACTGCT 
TCCAACTACC TGGACAGGAC AGAACAGTGG AGCAAACTGT CCCCTGAAAC AAAGGACCAT 
GCTGTGGATC TGATCCAAAA AGGATACATG AGGATCCAGC AGTTTCGGAA GAATGATGGC 
TCCTTTGGGG CTTGGTTACA CCGGGACAGC AGCACCTGGC TGACTGCCTT CGTGCTGAAG 
ATTCTGAGTT TGGCCCAGGA ACAGGTGGGC AACTCCCCGG AGAAGCTGCA GGAGACGGCT 
AGCTGGCTGC TGGCCCAGCA GCTGGGTGAT GGCTCCTTCC ACGACCCATG TCCAGTCATC 
CACAGAGCAA TGCAGGGGGG CTTGGTGGGG TCTGACGAGA CAGTGGCACT GACCGCCTTT 
GTGGTCATTG CCCTTCACCA TGGGTTGGAC GTCTTCCAGG ATGACGATGC GAAGCAGCTG 
AAGAACAGAG TGGAAGCCTC CATCACCAAG GCAAACTCAT TCTTGGGGCA GAAGGCAAGT 
GCTGGGCTCC TGGGTGCCCA TGCCGCCGCC ATCACAGCCT ATGCCCTTAC GCTGACCAAG 
GCCTCGGAGG ACCTGCGGAA TGTTGCCCAC AACAGCCTGA TGGCCATGGC TGAGGAAACA 
GGGGAACACC TCTACTGGGG CTTAGTCCTT GGCTCTCAGG ACAAAGTTGT GTTGCGCCCC 
ACAGCCCCCC GTAGCCCTAC AGAACCTGTG CCCCAGGCCC CAGCCTTGTG GATCGAAACC 
ACAGCCTATG CCCTGCTCCA CCTGCTTCTG CGGGAGGGAA AGGGAAAAAT GGCTGACAAG 
GCTGCATCCT GGCTCACCCA CCAGGGAAGC TTCCATGGGG CATTCCGCAG TACCCAGGAC 
ACTGTGGTCA CCCTGGATGC CCTGTCTGCC TACTGGATCG CTTCGCACAC CACTGAGGAG 
AAAGCACTGA ACGTGACGCT CAGCTCCATG GGCCGCAATG GGCTTAAGAC CCACGGGCTA 
CACTTGAACA ACCACCAAGT CAAGGGCCTG GAGGAGGAGC TAAAGTTCTC CCTGGGCAGC 
ACAATCAGTG TCAAGGTGGA AGGAAACAGC AAAGGCACCT TGAAGATCCT TCGTACCTAC 
AATGTCCTGG ACATGAAGAA CACCACATGC CAGGACCTTC AGATAGAAGT GAAGGTCACA 
GGCGCTGTGG AATACGCATG GGATGCCAAT GAAGACTACG AAGACTACTA TGACATGCCA 
GCCGCAGATG ACCCCAGCGT CCCCTTGCAG CCTGTCACGC CCCTGCAGCT TTTTGAGGGT 
CGTAGGAGCC GCCGCAGGAG GGAGGCCCCC AAGGTGGTCG AAGAGCAGGA GTCCAGAGTT 
CAGTACACTG TGTGTATCTG GCGAAATGGC AAGCTGGGGC TGTCTGGCAT GGCCATCGCA 
GACATCACCC TCCTAAGTGG ATTCCACGCC CTGAGGGCTG ACCTGGAGAA GCTGACCTCT 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACTGACGGGC CCCATGTCCT GTTGTACTTT 
GACTCGGTCC CTACCACCCG GGAGTGTGTG GGCTTCGGAG CCTCGCAGGA GGTGGTTGTG 
GGACTGGTGC AGCCATCCAG TGCTGTCCTG TATGACTACT ACAGCCCTGA TCACAAGTGC 
TCTGTGTTTT ATGCTGCACC CACCAAGAGC CAGCTCCTGG CCACACTGTG CTCTGGAGAT 
GTATGCCAGT GCGCTGAGGG GAAGTGCCCT CGACTGCTAA GGTCACTGGA GCGAAGGGTG 
GAGGACAAGG ACGGCTACCG GATGAGGTTC GCCTGCTATT ATCCCCGAGT GGAGTATGGC 
TTCACGGTTA AGGTTCTTCG AGAAGATGGC AGAGCTGCCT TCCGTCTCTT TGAGTCCAAG 
ATCACCCAAG TCCTGCATTT CAGAAAGGAC ACCATGGCCT CCATAGGTCA GACCCGCAAC 
TTCCTGAGCC GGGCCTCTTG CCGCCTTCGT TTGGAGCCTA ACAAAGAGTA CTTGATCATG 
GGGATGGACG GGGAAACCAG TGACAACAAG GGAGACCCCC AGTACTTGCT GGACTCAAAT 
ACCTGGATTG AGGAGATGCC TTCAGAACAA ATGTGCAAGA GCACCCGCCA TCGGGCAGCC 
TGTTTCCAGC TCAAAGACTT CCTGATGGAG TTCAGCAGCC GGGGGTGCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C4b ( XM_006523531.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523594.1 Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X1, mRNA.

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CloneID	OMu53047
Clone ID Related Accession (Same CDS sequence)	XM_006523531.3 , XM_006523531.4
Accession Version	XM_006523531.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	complement C4-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523531.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCTCC TCTGGGGGCT GGCCTGGGTG TTCAGCTTCT GTGCCTCATC CCTGCAGAAG 
CCCAGGTTGC TCCTGTTTTC CCCTTCTGTG GTTAATTTGG GGACCCCCCT GTCGGTGGGG 
GTACAGCTCC TGGATGCCCC TCCAGGACAG GAGGTAAAAG GATCAGTGTT CCTCAGAAAC 
CCAAAGGGTG GTTCCTGCTC CCCAAAGAAG GACTTTAAGC TGAGCTCGGG AGATGACTTT 
GTGCTGCTCA GCCTTGAGGT CCCACTGGAA GATGTGAGGA GCTGTGGCCT CTTTGACCTG 
CGCAGAGCCC CCCACATCCA GCTGGTAGCT CAGTCTCCGT GGCTAAGGAA CACAGCTTTC 
AAAGCCACAG AGACTCAGGG TGTCAACTTG CTCTTCTCTT CCCGACGAGG CCACATCTTT 
GTGCAGACCG ATCAGCCTAT CTATAATCCG GGGCAGCGGG TTCGTTATCG GGTCTTTGCA 
CTGGATCAAA AGATGCGCCC ATCCACTGAT TTCCTCACCA TCACAGTGGA GAACTCCCAT 
GGCCTCCGTG TACTCAAGAA GGAGATATTT ACTTCCACAT CCATCTTCCA AGATGCCTTC 
ACCATTCCAG ACATCTCAGA GCCTGGGACC TGGAAGATCT CAGCTAGGTT CTCAGATGGA 
CTGGAGTCCA ATAGGAGCAC CCACTTTGAA GTGAAGAAGT ATGTCCTTCC CAACTTCGAG 
GTGAAGATTA CTCCTTGGAA GCCATATATC CTGATGGTGC CCAGCAACAG TGATGAAATC 
CAATTAGACA TCCAGGCCAG GTACATCTAT GGGAAGCCCG TGCAGGGCGT GGCATACACA 
CGGTTTGCGC TCATGGATGA GCAAGGGAAG AGGACTTTCC TTCGGGGCCT AGAGACGCAG 
GCCAAGTTGG TGGAAGGCCG GACCCACATT TCCATCTCAA AGGACCAGTT CCAGGCTGCC 
CTGGATAAAA TCAATATTGG GGTCAGAGAC CTGGAGGGGC TGCGTCTCTA TGCTGCTACA 
GCTGTCATCG AGTCTCCAGG AGGAGAGATG GAGGAGGCAG AACTCACGTC CTGGCGCTTT 
GTATCATCTG CCTTCTCCTT GGATCTCAGC CGCACTAAGA GGCATCTTGT GCCTGGGGCC 
CACTTCCTGC TGCAGGCTCT GGTCCAAGAA ATGTCAGGCT CTGAAGCCTC CAACGTTCCT 
GTTAAAGTCT CTGCCACATT GGTGTCAGGC TCTGACTCCC AAGTCCTTGA CATTCAACAG 
AGCACCAATG GAATTGGCCA AGTCAGCATT TCCTTCCCCA TCCCACCAAC TGTCACAGAA 
CTTCGACTCT TGGTGTCTGC GGGCTCCCTC TACCCAGCCA TAGCCAGGCT CACCGTGCAA 
GCCCCACCTT CAAGAGGCAC TGGCTTTCTT TCTATTGAGC CACTAGACCC TCGGTCCCCT 
AGTGTGGGGG ACACCTTTAT CCTAAACCTT CAACCTGTGG GCATCCCTGC ACCTACCTTC 
TCTCATTACT ACTACATGAT CATCTCCAGA GGCCAGATCA TGGCTATGGG TCGGGAGCCC 
CGGAAGACTG TGACCTCCGT CTCTGTGTTG GTGGACCATC AGCTGGCTCC CTCGTTCTAC 
TTTGTGGCTT ACTTCTATCA CCAAGGACAC CCGGTGGCCA ACTCTCTGCT CATCAACATC 
CAATCCAGGG ACTGTGAGGG CAAGCTGCAA TTGAAGGTGG ATGGTGCCAA GGAGTATCGT 
AATGCGGACA TGATGAAGCT CCGAATTCAA ACTGACTCCA AAGCCCTGGT GGCACTGGGA 
GCTGTGGACA TGGCTCTGTA TGCTGTGGGT GGTCGGTCTC ACAAACCCCT CGACATGAGC 
AAGGTCTTTG AAGTAATCAA CAGCTACAAT GTTGGCTGTG GTCCTGGAGG TGGGGATGAT 
GCCCTTCAGG TGTTCCAGGA TGCTGGTCTG GCCTTTTCTG ATGGTGATCG ACTAACTCAA 
ACCAGAGAGG ACCTGAGCTG TCCCAAGGAG AAGAAAAGTC GGCAAAAGAG AAATGTTAAC 
TTCCAGAAGG CTGTCAGTGA GAAGTTGGGC CAGTATTCTT CTCCAGATGC CAAGCGCTGC 
TGCCAAGACG GGATGACGAA GCTGCCCATG AAGCGTACCT GTGAGCAGCG GGCTGCCCGT 
GTGCCTCAGC AGGCCTGCCG TGAGCCCTTC TTGTCCTGTT GCAAGTTTGC TGAGGACCTT 
CGCAGGAACC AGACCAGGAG CCAGGCACAC CTTGCCCGAA ACAACCACAA CATGCTGCAG 
GAGGAAGACT TGATAGATGA AGACGACATT CTTGTGCGCA CCTCCTTCCC AGAGAACTGG 
CTCTGGAGAG TGGAACCTGT AGACAGCTCC AAACTGTTGA CAGTGTGGCT TCCTGATTCT 
ATGACCACAT GGGAGATTCA TGGTGTGAGC CTGTCCAAAA GCAAAGGTCT GTGTGTAGCC 
AAGCCAACTC GTGTTCGAGT GTTCAGAAAA TTCCACCTGC ACCTGCGCCT GCCCATCTCC 
ATCCGCCGCT TTGAGCAGTT TGAATTACGG CCTGTTCTTT ACAACTATCT GAATGATGAT 
GTGGCTGTGA GTGTCCATGT GACCCCAGTT GAGGGGCTGT GCCTGGCTGG TGGTGGAATG 
ATGGCCCAGC AGGTGACAGT GCCAGCAGGT TCTGCCCGGC CTGTGGCCTT CTCTGTGGTA 
CCCACAGCTG CTGCCAACGT GCCCCTGAAG GTGGTGGCTC GAGGGGTTTT TGATTTAGGG 
GATGCTGTGT CTAAGATTCT CCAAATTGAG AAGGAAGGAG CCATCCACAG AGAAGAGTTA 
GTCTACAACC TCGACCCCCT AAATAACCTG GGTCGGACTT TGGAGATTCC TGGCAGCTCG 
GATCCCAACA TCGTCCCTGA CGGAGACTTC AGCAGCTTAG TCAGGGTTAC AGCCTCGGAA 
CCCTTGGAGA CTATGGGTTC TGAAGGTGCC TTGTCCCCAG GAGGTGTGGC CTCCCTCCTG 
AGGCTTCCCC AGGGCTGTGC AGAGCAAACC ATGATCTATT TGGCTCCTAC CCTGACTGCT 
TCCAACTACC TGGACAGGAC AGAACAGTGG AGCAAACTGT CCCCTGAAAC AAAGGACCAT 
GCTGTGGATC TGATCCAAAA AGGATACATG AGGATCCAGC AGTTTCGGAA GAATGATGGC 
TCCTTTGGGG CTTGGTTACA CCGGGACAGC AGCACCTGGC TGACTGCCTT CGTGCTGAAG 
ATTCTGAGTT TGGCCCAGGA ACAGGTGGGC AACTCCCCGG AGAAGCTGCA GGAGACGGCT 
AGCTGGCTGC TGGCCCAGCA GCTGGGTGAT GGCTCCTTCC ACGACCCATG TCCAGTCATC 
CACAGAGCAA TGCAGGGGGG CTTGGTGGGG TCTGACGAGA CAGTGGCACT GACCGCCTTT 
GTGGTCATTG CCCTTCACCA TGGGTTGGAC GTCTTCCAGG ATGACGATGC GAAGCAGCTG 
AAGAACAGAG TGGAAGCCTC CATCACCAAG GCAAACTCAT TCTTGGGGCA GAAGGCAAGT 
GCTGGGCTCC TGGGTGCCCA TGCCGCCGCC ATCACAGCCT ATGCCCTTAC GCTGACCAAG 
GCCTCGGAGG ACCTGCGGAA TGTTGCCCAC AACAGCCTGA TGGCCATGGC TGAGGAAACA 
GGGGAACACC TCTACTGGGG CTTAGTCCTT GGCTCTCAGG ACAAAGTTGT GTTGCGCCCC 
ACAGCCCCCC GTAGCCCTAC AGAACCTGTG CCCCAGGCCC CAGCCTTGTG GATCGAAACC 
ACAGCCTATG CCCTGCTCCA CCTGCTTCTG CGGGAGGGAA AGGGAAAAAT GGCTGACAAG 
GCTGCATCCT GGCTCACCCA CCAGGGAAGC TTCCATGGGG CATTCCGCAG TACCCAGGAC 
ACTGTGGTCA CCCTGGATGC CCTGTCTGCC TACTGGATCG CTTCGCACAC CACTGAGGAG 
AAAGCACTGA ACGTGACGCT CAGCTCCATG GGCCGCAATG GGCTTAAGAC CCACGGGCTA 
CACTTGAACA ACCACCAAGT CAAGGGCCTG GAGGAGGAGC TAAAGTTCTC CCTGGGCAGC 
ACAATCAGTG TCAAGGTGGA AGGAAACAGC AAAGGCACCT TGAAGATCCT TCGTACCTAC 
AATGTCCTGG ACATGAAGAA CACCACATGC CAGGACCTTC AGATAGAAGT GAAGGTCACA 
GGCGCTGTGG AATACGCATG GGATGCCAAT GAAGACTACG AAGACTACTA TGACATGCCA 
GCCGCAGATG ACCCCAGCGT CCCCTTGCAG CCTGTCACGC CCCTGCAGCT TTTTGAGGGT 
CGTAGGAGCC GCCGCAGGAG GGAGGCCCCC AAGGTGGTCG AAGAGCAGGA GTCCAGAGTT 
CAGTACACTG TGTGTATCTG GCGAAATGGC AAGCTGGGGC TGTCTGGCAT GGCCATCGCA 
GACATCACCC TCCTAAGTGG ATTCCACGCC CTGAGGGCTG ACCTGGAGAA GCTGACCTCT 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACTGACGGGC CCCATGTCCT GTTGTACTTT 
GACTCGGTCC CTACCACCCG GGAGTGTGTG GGCTTCGGAG CCTCGCAGGA GGTGGTTGTG 
GGACTGGTGC AGCCATCCAG TGCTGTCCTG TATGACTACT ACAGCCCTGA TCACAAGTGC 
TCTGTGTTTT ATGCTGCACC CACCAAGAGC CAGCTCCTGG CCACACTGTG CTCTGGAGAT 
GTATGCCAGT GCGCTGAGGG GAAGTGCCCT CGACTGCTAA GGTCACTGGA GCGAAGGGTG 
GAGGACAAGG ACGGCTACCG GATGAGGTTC GCCTGCTATT ATCCCCGAGT GGAGTATGGC 
TTCACGGTTA AGGTTCTTCG AGAAGATGGC AGAGCTGCCT TCCGTCTCTT TGAGTCCAAG 
ATCACCCAAG TCCTGCATTT CAGAAAGGAC ACCATGGCCT CCATAGGTCA GACCCGCAAC 
TTCCTGAGCC GGGCCTCTTG CCGCCTTCGT TTGGAGCCTA ACAAAGAGTA CTTGATCATG 
GGGATGGACG GGGAAACCAG TGACAACAAG GGAGACCCCC AGTACTTGCT GGACTCAAAT 
ACCTGGATTG AGGAGATGCC TTCAGAACAA ATGTGCAAGA GCACCCGCCA TCGGGCAGCC 
TGTTTCCAGC TCAAAGACTT CCTGATGGAG TTCAGCAGCC GGGGGTGCCA GGTGCGGCAG 
TCTGCCACCC ACCCAGGATT TTCAGGCCTG AGCCTTGGAC CAGAAAGCTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523594.1
CDS	77..5353
Translation	MRLLWGLAWVFSFCASSLQKPRLLLFSPSVVNLGTPLSVGVQLLDAPPGQEVKGSVFLRNPKGGSCSPKKDFKLSSGDDFVLLSLEVPLEDVRSCGLFDLRRAPHIQLVAQSPWLRNTAFKATETQGVNLLFSSRRGHIFVQTDQPIYNPGQRVRYRVFALDQKMRPSTDFLTITVENSHGLRVLKKEIFTSTSIFQDAFTIPDISEPGTWKISARFSDGLESNRSTHFEVKKYVLPNFEVKITPWKPYILMVPSNSDEIQLDIQARYIYGKPVQGVAYTRFALMDEQGKRTFLRGLETQAKLVEGRTHISISKDQFQAALDKINIGVRDLEGLRLYAATAVIESPGGEMEEAELTSWRFVSSAFSLDLSRTKRHLVPGAHFLLQALVQEMSGSEASNVPVKVSATLVSGSDSQVLDIQQSTNGIGQVSISFPIPPTVTELRLLVSAGSLYPAIARLTVQAPPSRGTGFLSIEPLDPRSPSVGDTFILNLQPVGIPAPTFSHYYYMIISRGQIMAMGREPRKTVTSVSVLVDHQLAPSFYFVAYFYHQGHPVANSLLINIQSRDCEGKLQLKVDGAKEYRNADMMKLRIQTDSKALVALGAVDMALYAVGGRSHKPLDMSKVFEVINSYNVGCGPGGGDDALQVFQDAGLAFSDGDRLTQTREDLSCPKEKKSRQKRNVNFQKAVSEKLGQYSSPDAKRCCQDGMTKLPMKRTCEQRAARVPQQACREPFLSCCKFAEDLRRNQTRSQAHLARNNHNMLQEEDLIDEDDILVRTSFPENWLWRVEPVDSSKLLTVWLPDSMTTWEIHGVSLSKSKGLCVAKPTRVRVFRKFHLHLRLPISIRRFEQFELRPVLYNYLNDDVAVSVHVTPVEGLCLAGGGMMAQQVTVPAGSARPVAFSVVPTAAANVPLKVVARGVFDLGDAVSKILQIEKEGAIHREELVYNLDPLNNLGRTLEIPGSSDPNIVPDGDFSSLVRVTASEPLETMGSEGALSPGGVASLLRLPQGCAEQTMIYLAPTLTASNYLDRTEQWSKLSPETKDHAVDLIQKGYMRIQQFRKNDGSFGAWLHRDSSTWLTAFVLKILSLAQEQVGNSPEKLQETASWLLAQQLGDGSFHDPCPVIHRAMQGGLVGSDETVALTAFVVIALHHGLDVFQDDDAKQLKNRVEASITKANSFLGQKASAGLLGAHAAAITAYALTLTKASEDLRNVAHNSLMAMAEETGEHLYWGLVLGSQDKVVLRPTAPRSPTEPVPQAPALWIETTAYALLHLLLREGKGKMADKAASWLTHQGSFHGAFRSTQDTVVTLDALSAYWIASHTTEEKALNVTLSSMGRNGLKTHGLHLNNHQVKGLEEELKFSLGSTISVKVEGNSKGTLKILRTYNVLDMKNTTCQDLQIEVKVTGAVEYAWDANEDYEDYYDMPAADDPSVPLQPVTPLQLFEGRRSRRRREAPKVVEEQESRVQYTVCIWRNGKLGLSGMAIADITLLSGFHALRADLEKLTSLSDRYVSHFETDGPHVLLYFDSVPTTRECVGFGASQEVVVGLVQPSSAVLYDYYSPDHKCSVFYAAPTKSQLLATLCSGDVCQCAEGKCPRLLRSLERRVEDKDGYRMRFACYYPRVEYGFTVKVLREDGRAAFRLFESKITQVLHFRKDTMASIGQTRNFLSRASCRLRLEPNKEYLIMGMDGETSDNKGDPQYLLDSNTWIEEMPSEQMCKSTRHRAACFQLKDFLMEFSSRGCQVRQSATHPGFSGLSLGPESSA

Target ORF information:

RefSeq Version	XM_006523531.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523531.4

ORF Insert Sequence:

ATGCGGCTCC TCTGGGGGCT GGCCTGGGTG TTCAGCTTCT GTGCCTCATC CCTGCAGAAG 
CCCAGGTTGC TCCTGTTTTC CCCTTCTGTG GTTAATTTGG GGACCCCCCT GTCGGTGGGG 
GTACAGCTCC TGGATGCCCC TCCAGGACAG GAGGTAAAAG GATCAGTGTT CCTCAGAAAC 
CCAAAGGGTG GTTCCTGCTC CCCAAAGAAG GACTTTAAGC TGAGCTCGGG AGATGACTTT 
GTGCTGCTCA GCCTTGAGGT CCCACTGGAA GATGTGAGGA GCTGTGGCCT CTTTGACCTG 
CGCAGAGCCC CCCACATCCA GCTGGTAGCT CAGTCTCCGT GGCTAAGGAA CACAGCTTTC 
AAAGCCACAG AGACTCAGGG TGTCAACTTG CTCTTCTCTT CCCGACGAGG CCACATCTTT 
GTGCAGACCG ATCAGCCTAT CTATAATCCG GGGCAGCGGG TTCGTTATCG GGTCTTTGCA 
CTGGATCAAA AGATGCGCCC ATCCACTGAT TTCCTCACCA TCACAGTGGA GAACTCCCAT 
GGCCTCCGTG TACTCAAGAA GGAGATATTT ACTTCCACAT CCATCTTCCA AGATGCCTTC 
ACCATTCCAG ACATCTCAGA GCCTGGGACC TGGAAGATCT CAGCTAGGTT CTCAGATGGA 
CTGGAGTCCA ATAGGAGCAC CCACTTTGAA GTGAAGAAGT ATGTCCTTCC CAACTTCGAG 
GTGAAGATTA CTCCTTGGAA GCCATATATC CTGATGGTGC CCAGCAACAG TGATGAAATC 
CAATTAGACA TCCAGGCCAG GTACATCTAT GGGAAGCCCG TGCAGGGCGT GGCATACACA 
CGGTTTGCGC TCATGGATGA GCAAGGGAAG AGGACTTTCC TTCGGGGCCT AGAGACGCAG 
GCCAAGTTGG TGGAAGGCCG GACCCACATT TCCATCTCAA AGGACCAGTT CCAGGCTGCC 
CTGGATAAAA TCAATATTGG GGTCAGAGAC CTGGAGGGGC TGCGTCTCTA TGCTGCTACA 
GCTGTCATCG AGTCTCCAGG AGGAGAGATG GAGGAGGCAG AACTCACGTC CTGGCGCTTT 
GTATCATCTG CCTTCTCCTT GGATCTCAGC CGCACTAAGA GGCATCTTGT GCCTGGGGCC 
CACTTCCTGC TGCAGGCTCT GGTCCAAGAA ATGTCAGGCT CTGAAGCCTC CAACGTTCCT 
GTTAAAGTCT CTGCCACATT GGTGTCAGGC TCTGACTCCC AAGTCCTTGA CATTCAACAG 
AGCACCAATG GAATTGGCCA AGTCAGCATT TCCTTCCCCA TCCCACCAAC TGTCACAGAA 
CTTCGACTCT TGGTGTCTGC GGGCTCCCTC TACCCAGCCA TAGCCAGGCT CACCGTGCAA 
GCCCCACCTT CAAGAGGCAC TGGCTTTCTT TCTATTGAGC CACTAGACCC TCGGTCCCCT 
AGTGTGGGGG ACACCTTTAT CCTAAACCTT CAACCTGTGG GCATCCCTGC ACCTACCTTC 
TCTCATTACT ACTACATGAT CATCTCCAGA GGCCAGATCA TGGCTATGGG TCGGGAGCCC 
CGGAAGACTG TGACCTCCGT CTCTGTGTTG GTGGACCATC AGCTGGCTCC CTCGTTCTAC 
TTTGTGGCTT ACTTCTATCA CCAAGGACAC CCGGTGGCCA ACTCTCTGCT CATCAACATC 
CAATCCAGGG ACTGTGAGGG CAAGCTGCAA TTGAAGGTGG ATGGTGCCAA GGAGTATCGT 
AATGCGGACA TGATGAAGCT CCGAATTCAA ACTGACTCCA AAGCCCTGGT GGCACTGGGA 
GCTGTGGACA TGGCTCTGTA TGCTGTGGGT GGTCGGTCTC ACAAACCCCT CGACATGAGC 
AAGGTCTTTG AAGTAATCAA CAGCTACAAT GTTGGCTGTG GTCCTGGAGG TGGGGATGAT 
GCCCTTCAGG TGTTCCAGGA TGCTGGTCTG GCCTTTTCTG ATGGTGATCG ACTAACTCAA 
ACCAGAGAGG ACCTGAGCTG TCCCAAGGAG AAGAAAAGTC GGCAAAAGAG AAATGTTAAC 
TTCCAGAAGG CTGTCAGTGA GAAGTTGGGC CAGTATTCTT CTCCAGATGC CAAGCGCTGC 
TGCCAAGACG GGATGACGAA GCTGCCCATG AAGCGTACCT GTGAGCAGCG GGCTGCCCGT 
GTGCCTCAGC AGGCCTGCCG TGAGCCCTTC TTGTCCTGTT GCAAGTTTGC TGAGGACCTT 
CGCAGGAACC AGACCAGGAG CCAGGCACAC CTTGCCCGAA ACAACCACAA CATGCTGCAG 
GAGGAAGACT TGATAGATGA AGACGACATT CTTGTGCGCA CCTCCTTCCC AGAGAACTGG 
CTCTGGAGAG TGGAACCTGT AGACAGCTCC AAACTGTTGA CAGTGTGGCT TCCTGATTCT 
ATGACCACAT GGGAGATTCA TGGTGTGAGC CTGTCCAAAA GCAAAGGTCT GTGTGTAGCC 
AAGCCAACTC GTGTTCGAGT GTTCAGAAAA TTCCACCTGC ACCTGCGCCT GCCCATCTCC 
ATCCGCCGCT TTGAGCAGTT TGAATTACGG CCTGTTCTTT ACAACTATCT GAATGATGAT 
GTGGCTGTGA GTGTCCATGT GACCCCAGTT GAGGGGCTGT GCCTGGCTGG TGGTGGAATG 
ATGGCCCAGC AGGTGACAGT GCCAGCAGGT TCTGCCCGGC CTGTGGCCTT CTCTGTGGTA 
CCCACAGCTG CTGCCAACGT GCCCCTGAAG GTGGTGGCTC GAGGGGTTTT TGATTTAGGG 
GATGCTGTGT CTAAGATTCT CCAAATTGAG AAGGAAGGAG CCATCCACAG AGAAGAGTTA 
GTCTACAACC TCGACCCCCT AAATAACCTG GGTCGGACTT TGGAGATTCC TGGCAGCTCG 
GATCCCAACA TCGTCCCTGA CGGAGACTTC AGCAGCTTAG TCAGGGTTAC AGCCTCGGAA 
CCCTTGGAGA CTATGGGTTC TGAAGGTGCC TTGTCCCCAG GAGGTGTGGC CTCCCTCCTG 
AGGCTTCCCC AGGGCTGTGC AGAGCAAACC ATGATCTATT TGGCTCCTAC CCTGACTGCT 
TCCAACTACC TGGACAGGAC AGAACAGTGG AGCAAACTGT CCCCTGAAAC AAAGGACCAT 
GCTGTGGATC TGATCCAAAA AGGATACATG AGGATCCAGC AGTTTCGGAA GAATGATGGC 
TCCTTTGGGG CTTGGTTACA CCGGGACAGC AGCACCTGGC TGACTGCCTT CGTGCTGAAG 
ATTCTGAGTT TGGCCCAGGA ACAGGTGGGC AACTCCCCGG AGAAGCTGCA GGAGACGGCT 
AGCTGGCTGC TGGCCCAGCA GCTGGGTGAT GGCTCCTTCC ACGACCCATG TCCAGTCATC 
CACAGAGCAA TGCAGGGGGG CTTGGTGGGG TCTGACGAGA CAGTGGCACT GACCGCCTTT 
GTGGTCATTG CCCTTCACCA TGGGTTGGAC GTCTTCCAGG ATGACGATGC GAAGCAGCTG 
AAGAACAGAG TGGAAGCCTC CATCACCAAG GCAAACTCAT TCTTGGGGCA GAAGGCAAGT 
GCTGGGCTCC TGGGTGCCCA TGCCGCCGCC ATCACAGCCT ATGCCCTTAC GCTGACCAAG 
GCCTCGGAGG ACCTGCGGAA TGTTGCCCAC AACAGCCTGA TGGCCATGGC TGAGGAAACA 
GGGGAACACC TCTACTGGGG CTTAGTCCTT GGCTCTCAGG ACAAAGTTGT GTTGCGCCCC 
ACAGCCCCCC GTAGCCCTAC AGAACCTGTG CCCCAGGCCC CAGCCTTGTG GATCGAAACC 
ACAGCCTATG CCCTGCTCCA CCTGCTTCTG CGGGAGGGAA AGGGAAAAAT GGCTGACAAG 
GCTGCATCCT GGCTCACCCA CCAGGGAAGC TTCCATGGGG CATTCCGCAG TACCCAGGAC 
ACTGTGGTCA CCCTGGATGC CCTGTCTGCC TACTGGATCG CTTCGCACAC CACTGAGGAG 
AAAGCACTGA ACGTGACGCT CAGCTCCATG GGCCGCAATG GGCTTAAGAC CCACGGGCTA 
CACTTGAACA ACCACCAAGT CAAGGGCCTG GAGGAGGAGC TAAAGTTCTC CCTGGGCAGC 
ACAATCAGTG TCAAGGTGGA AGGAAACAGC AAAGGCACCT TGAAGATCCT TCGTACCTAC 
AATGTCCTGG ACATGAAGAA CACCACATGC CAGGACCTTC AGATAGAAGT GAAGGTCACA 
GGCGCTGTGG AATACGCATG GGATGCCAAT GAAGACTACG AAGACTACTA TGACATGCCA 
GCCGCAGATG ACCCCAGCGT CCCCTTGCAG CCTGTCACGC CCCTGCAGCT TTTTGAGGGT 
CGTAGGAGCC GCCGCAGGAG GGAGGCCCCC AAGGTGGTCG AAGAGCAGGA GTCCAGAGTT 
CAGTACACTG TGTGTATCTG GCGAAATGGC AAGCTGGGGC TGTCTGGCAT GGCCATCGCA 
GACATCACCC TCCTAAGTGG ATTCCACGCC CTGAGGGCTG ACCTGGAGAA GCTGACCTCT 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACTGACGGGC CCCATGTCCT GTTGTACTTT 
GACTCGGTCC CTACCACCCG GGAGTGTGTG GGCTTCGGAG CCTCGCAGGA GGTGGTTGTG 
GGACTGGTGC AGCCATCCAG TGCTGTCCTG TATGACTACT ACAGCCCTGA TCACAAGTGC 
TCTGTGTTTT ATGCTGCACC CACCAAGAGC CAGCTCCTGG CCACACTGTG CTCTGGAGAT 
GTATGCCAGT GCGCTGAGGG GAAGTGCCCT CGACTGCTAA GGTCACTGGA GCGAAGGGTG 
GAGGACAAGG ACGGCTACCG GATGAGGTTC GCCTGCTATT ATCCCCGAGT GGAGTATGGC 
TTCACGGTTA AGGTTCTTCG AGAAGATGGC AGAGCTGCCT TCCGTCTCTT TGAGTCCAAG 
ATCACCCAAG TCCTGCATTT CAGAAAGGAC ACCATGGCCT CCATAGGTCA GACCCGCAAC 
TTCCTGAGCC GGGCCTCTTG CCGCCTTCGT TTGGAGCCTA ACAAAGAGTA CTTGATCATG 
GGGATGGACG GGGAAACCAG TGACAACAAG GGAGACCCCC AGTACTTGCT GGACTCAAAT 
ACCTGGATTG AGGAGATGCC TTCAGAACAA ATGTGCAAGA GCACCCGCCA TCGGGCAGCC 
TGTTTCCAGC TCAAAGACTT CCTGATGGAG TTCAGCAGCC GGGGGTGCCA GGTGCGGCAG 
TCTGCCACCC ACCCAGGATT TTCAGGCCTG AGCCTTGGAC CAGAAAGCTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C4b ( XM_006523532.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523595.1 Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu53048
Clone ID Related Accession (Same CDS sequence)	XM_006523532.4 , XM_006523532.3
Accession Version	XM_006523532.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	complement C4-B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523532.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCTCC TCTGGGGGCT GGCCTGGGTG TTCAGCTTCT GTGCCTCATC CCTGCAGAAG 
CCCAGGTTGC TCCTGTTTTC CCCTTCTGTG GTTAATTTGG GGACCCCCCT GTCGGTGGGG 
GTACAGCTCC TGGATGCCCC TCCAGGACAG GAGGTAAAAG GATCAGTGTT CCTCAGAAAC 
CCAAAGGGTG GTTCCTGCTC CCCAAAGAAG GACTTTAAGC TGAGCTCGGG AGATGACTTT 
GTGCTGCTCA GCCTTGAGGT CCCACTGGAA GATGTGAGGA GCTGTGGCCT CTTTGACCTG 
CGCAGAGCCC CCCACATCCA GCTGGTAGCT CAGTCTCCGT GGCTAAGGAA CACAGCTTTC 
AAAGCCACAG AGACTCAGGG TGTCAACTTG CTCTTCTCTT CCCGACGAGG CCACATCTTT 
GTGCAGACCG ATCAGCCTAT CTATAATCCG GGGCAGCGGG TTCGTTATCG GGTCTTTGCA 
CTGGATCAAA AGATGCGCCC ATCCACTGAT TTCCTCACCA TCACAGTGGA GAACTCCCAT 
GGCCTCCGTG TACTCAAGAA GGAGATATTT ACTTCCACAT CCATCTTCCA AGATGCCTTC 
ACCATTCCAG ACATCTCAGA GCCTGGGACC TGGAAGATCT CAGCTAGGTT CTCAGATGGA 
CTGGAGTCCA ATAGGAGCAC CCACTTTGAA GTGAAGAAGT ATGTCCTTCC CAACTTCGAG 
GTGAAGATTA CTCCTTGGAA GCCATATATC CTGATGGTGC CCAGCAACAG TGATGAAATC 
CAATTAGACA TCCAGGCCAG GTACATCTAT GGGAAGCCCG TGCAGGGCGT GGCATACACA 
CGGTTTGCGC TCATGGATGA GCAAGGGAAG AGGACTTTCC TTCGGGGCCT AGAGACGCAG 
GCCAAGTTGG TGGAAGGCCG GACCCACATT TCCATCTCAA AGGACCAGTT CCAGGCTGCC 
CTGGATAAAA TCAATATTGG GGTCAGAGAC CTGGAGGGGC TGCGTCTCTA TGCTGCTACA 
GCTGTCATCG AGTCTCCAGG AGGAGAGATG GAGGAGGCAG AACTCACGTC CTGGCGCTTT 
GTATCATCTG CCTTCTCCTT GGATCTCAGC CGCACTAAGA GGCATCTTGT GCCTGGGGCC 
CACTTCCTGC TGCAGGCTCT GGTCCAAGAA ATGTCAGGCT CTGAAGCCTC CAACGTTCCT 
GTTAAAGTCT CTGCCACATT GGTGTCAGGC TCTGACTCCC AAGTCCTTGA CATTCAACAG 
AGCACCAATG GAATTGGCCA AGTCAGCATT TCCTTCCCCA TCCCACCAAC TGTCACAGAA 
CTTCGACTCT TGGTGTCTGC GGGCTCCCTC TACCCAGCCA TAGCCAGGCT CACCGTGCAA 
GCCCCACCTT CAAGAGGCAC TGGCTTTCTT TCTATTGAGC CACTAGACCC TCGGTCCCCT 
AGTGTGGGGG ACACCTTTAT CCTAAACCTT CAACCTGTGG GCATCCCTGC ACCTACCTTC 
TCTCATTACT ACTACATGAT CATCTCCAGA GGCCAGATCA TGGCTATGGG TCGGGAGCCC 
CGGAAGACTG TGACCTCCGT CTCTGTGTTG GTGGACCATC AGCTGGCTCC CTCGTTCTAC 
TTTGTGGCTT ACTTCTATCA CCAAGGACAC CCGGTGGCCA ACTCTCTGCT CATCAACATC 
CAATCCAGGG ACTGTGAGGG CAAGCTGCAA TTGAAGGTGG ATGGTGCCAA GGAGTATCGT 
AATGCGGACA TGATGAAGCT CCGAATTCAA ACTGACTCCA AAGCCCTGGT GGCACTGGGA 
GCTGTGGACA TGGCTCTGTA TGCTGTGGGT GGTCGGTCTC ACAAACCCCT CGACATGAGC 
AAGGTCTTTG AAGTAATCAA CAGCTACAAT GTTGGCTGTG GTCCTGGAGG TGGGGATGAT 
GCCCTTCAGG TGTTCCAGGA TGCTGGTCTG GCCTTTTCTG ATGGTGATCG ACTAACTCAA 
ACCAGAGAGG ACCTGAGCTG TCCCAAGGAG AAGAAAAGTC GGCAAAAGAG AAATGTTAAC 
TTCCAGAAGG CTGTCAGTGA GAAGTTGGGC CAGTATTCTT CTCCAGATGC CAAGCGCTGC 
TGCCAAGACG GGATGACGAA GCTGCCCATG AAGCGTACCT GTGAGCAGCG GGCTGCCCGT 
GTGCCTCAGC AGGCCTGCCG TGAGCCCTTC TTGTCCTGTT GCAAGTTTGC TGAGGACCTT 
CGCAGGAACC AGACCAGGAG CCAGGCACAC CTTGCCCGAA ACAACCACAA CATGCTGCAG 
GAGGAAGACT TGATAGATGA AGACGACATT CTTGTGCGCA CCTCCTTCCC AGAGAACTGG 
CTCTGGAGAG TGGAACCTGT AGACAGCTCC AAACTGTTGA CAGTGTGGCT TCCTGATTCT 
ATGACCACAT GGGAGATTCA TGGTGTGAGC CTGTCCAAAA GCAAAGGTCT GTGTGTAGCC 
AAGCCAACTC GTGTTCGAGT GTTCAGAAAA TTCCACCTGC ACCTGCGCCT GCCCATCTCC 
ATCCGCCGCT TTGAGCAGTT TGAATTACGG CCTGTTCTTT ACAACTATCT GAATGATGAT 
GTGGCTGTGA GTGTCCATGT GACCCCAGTT GAGGGGCTGT GCCTGGCTGG TGGTGGAATG 
ATGGCCCAGC AGGTGACAGT GCCAGCAGGT TCTGCCCGGC CTGTGGCCTT CTCTGTGGTA 
CCCACAGCTG CTGCCAACGT GCCCCTGAAG GTGGTGGCTC GAGGGGTTTT TGATTTAGGG 
GATGCTGTGT CTAAGATTCT CCAAATTGAG AAGGAAGGAG CCATCCACAG AGAAGAGTTA 
GTCTACAACC TCGACCCCCT AAATAACCTG GGTCGGACTT TGGAGATTCC TGGCAGCTCG 
GATCCCAACA TCGTCCCTGA CGGAGACTTC AGCAGCTTAG TCAGGGTTAC AGCCTCGGAA 
CCCTTGGAGA CTATGGGTTC TGAAGGTGCC TTGTCCCCAG GAGGTGTGGC CTCCCTCCTG 
AGGCTTCCCC AGGGCTGTGC AGAGCAAACC ATGATCTATT TGGCTCCTAC CCTGACTGCT 
TCCAACTACC TGGACAGGAC AGAACAGTGG AGCAAACTGT CCCCTGAAAC AAAGGACCAT 
GCTGTGGATC TGATCCAAAA AGGATACATG AGGATCCAGC AGTTTCGGAA GAATGATGGC 
TCCTTTGGGG CTTGGTTACA CCGGGACAGC AGCACCTGGC TGACTGCCTT CGTGCTGAAG 
ATTCTGAGTT TGGCCCAGGA ACAGGTGGGC AACTCCCCGG AGAAGCTGCA GGAGACGGCT 
AGCTGGCTGC TGGCCCAGCA GCTGGGTGAT GGCTCCTTCC ACGACCCATG TCCAGTCATC 
CACAGAGCAA TGCAGGGGGG CTTGGTGGGG TCTGACGAGA CAGTGGCACT GACCGCCTTT 
GTGGTCATTG CCCTTCACCA TGGGTTGGAC GTCTTCCAGG ATGACGATGC GAAGCAGCTG 
AAGAACAGAG TGGAAGCCTC CATCACCAAG GCAAACTCAT TCTTGGGGCA GAAGGCAAGT 
GCTGGGCTCC TGGGTGCCCA TGCCGCCGCC ATCACAGCCT ATGCCCTTAC GCTGACCAAG 
GCCTCGGAGG ACCTGCGGAA TGTTGCCCAC AACAGCCTGA TGGCCATGGC TGAGGAAACA 
GGGGAACACC TCTACTGGGG CTTAGTCCTT GGCTCTCAGG ACAAAGTTGT GTTGCGCCCC 
ACAGCCCCCC GTAGCCCTAC AGAACCTGTG CCCCAGGCCC CAGCCTTGTG GATCGAAACC 
ACAGCCTATG CCCTGCTCCA CCTGCTTCTG CGGGAGGGAA AGGGAAAAAT GGCTGACAAG 
GCTGCATCCT GGCTCACCCA CCAGGGAAGC TTCCATGGGG CATTCCGCAG TACCCAGGAC 
ACTGTGGTCA CCCTGGATGC CCTGTCTGCC TACTGGATCG CTTCGCACAC CACTGAGGAG 
AAAGCACTGA ACGTGACGCT CAGCTCCATG GGCCGCAATG GGCTTAAGAC CCACGGGCTA 
CACTTGAACA ACCACCAAGT CAAGGGCCTG GAGGAGGAGC TAAAGATCCT TCGTACCTAC 
AATGTCCTGG ACATGAAGAA CACCACATGC CAGGACCTTC AGATAGAAGT GAAGGTCACA 
GGCGCTGTGG AATACGCATG GGATGCCAAT GAAGACTACG AAGACTACTA TGACATGCCA 
GCCGCAGATG ACCCCAGCGT CCCCTTGCAG CCTGTCACGC CCCTGCAGCT TTTTGAGGGT 
CGTAGGAGCC GCCGCAGGAG GGAGGCCCCC AAGGTGGTCG AAGAGCAGGA GTCCAGAGTT 
CAGTACACTG TGTGTATCTG GCGAAATGGC AAGCTGGGGC TGTCTGGCAT GGCCATCGCA 
GACATCACCC TCCTAAGTGG ATTCCACGCC CTGAGGGCTG ACCTGGAGAA GCTGACCTCT 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACTGACGGGC CCCATGTCCT GTTGTACTTT 
GACTCGGTCC CTACCACCCG GGAGTGTGTG GGCTTCGGAG CCTCGCAGGA GGTGGTTGTG 
GGACTGGTGC AGCCATCCAG TGCTGTCCTG TATGACTACT ACAGCCCTGA TCACAAGTGC 
TCTGTGTTTT ATGCTGCACC CACCAAGAGC CAGCTCCTGG CCACACTGTG CTCTGGAGAT 
GTATGCCAGT GCGCTGAGGG GAAGTGCCCT CGACTGCTAA GGTCACTGGA GCGAAGGGTG 
GAGGACAAGG ACGGCTACCG GATGAGGTTC GCCTGCTATT ATCCCCGAGT GGAGTATGGC 
TTCACGGTTA AGGTTCTTCG AGAAGATGGC AGAGCTGCCT TCCGTCTCTT TGAGTCCAAG 
ATCACCCAAG TCCTGCATTT CAGAAAGGAC ACCATGGCCT CCATAGGTCA GACCCGCAAC 
TTCCTGAGCC GGGCCTCTTG CCGCCTTCGT TTGGAGCCTA ACAAAGAGTA CTTGATCATG 
GGGATGGACG GGGAAACCAG TGACAACAAG GGAGACCCCC AGTACTTGCT GGACTCAAAT 
ACCTGGATTG AGGAGATGCC TTCAGAACAA ATGTGCAAGA GCACCCGCCA TCGGGCAGCC 
TGTTTCCAGC TCAAAGACTT CCTGATGGAG TTCAGCAGCC GGGGGTGCCA GGTGCGGCAG 
TCTGCCACCC ACCCAGGATT TTCAGGCCTG AGCCTTGGAC CAGAAAGCTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523595.1
CDS	77..5293
Translation	MRLLWGLAWVFSFCASSLQKPRLLLFSPSVVNLGTPLSVGVQLLDAPPGQEVKGSVFLRNPKGGSCSPKKDFKLSSGDDFVLLSLEVPLEDVRSCGLFDLRRAPHIQLVAQSPWLRNTAFKATETQGVNLLFSSRRGHIFVQTDQPIYNPGQRVRYRVFALDQKMRPSTDFLTITVENSHGLRVLKKEIFTSTSIFQDAFTIPDISEPGTWKISARFSDGLESNRSTHFEVKKYVLPNFEVKITPWKPYILMVPSNSDEIQLDIQARYIYGKPVQGVAYTRFALMDEQGKRTFLRGLETQAKLVEGRTHISISKDQFQAALDKINIGVRDLEGLRLYAATAVIESPGGEMEEAELTSWRFVSSAFSLDLSRTKRHLVPGAHFLLQALVQEMSGSEASNVPVKVSATLVSGSDSQVLDIQQSTNGIGQVSISFPIPPTVTELRLLVSAGSLYPAIARLTVQAPPSRGTGFLSIEPLDPRSPSVGDTFILNLQPVGIPAPTFSHYYYMIISRGQIMAMGREPRKTVTSVSVLVDHQLAPSFYFVAYFYHQGHPVANSLLINIQSRDCEGKLQLKVDGAKEYRNADMMKLRIQTDSKALVALGAVDMALYAVGGRSHKPLDMSKVFEVINSYNVGCGPGGGDDALQVFQDAGLAFSDGDRLTQTREDLSCPKEKKSRQKRNVNFQKAVSEKLGQYSSPDAKRCCQDGMTKLPMKRTCEQRAARVPQQACREPFLSCCKFAEDLRRNQTRSQAHLARNNHNMLQEEDLIDEDDILVRTSFPENWLWRVEPVDSSKLLTVWLPDSMTTWEIHGVSLSKSKGLCVAKPTRVRVFRKFHLHLRLPISIRRFEQFELRPVLYNYLNDDVAVSVHVTPVEGLCLAGGGMMAQQVTVPAGSARPVAFSVVPTAAANVPLKVVARGVFDLGDAVSKILQIEKEGAIHREELVYNLDPLNNLGRTLEIPGSSDPNIVPDGDFSSLVRVTASEPLETMGSEGALSPGGVASLLRLPQGCAEQTMIYLAPTLTASNYLDRTEQWSKLSPETKDHAVDLIQKGYMRIQQFRKNDGSFGAWLHRDSSTWLTAFVLKILSLAQEQVGNSPEKLQETASWLLAQQLGDGSFHDPCPVIHRAMQGGLVGSDETVALTAFVVIALHHGLDVFQDDDAKQLKNRVEASITKANSFLGQKASAGLLGAHAAAITAYALTLTKASEDLRNVAHNSLMAMAEETGEHLYWGLVLGSQDKVVLRPTAPRSPTEPVPQAPALWIETTAYALLHLLLREGKGKMADKAASWLTHQGSFHGAFRSTQDTVVTLDALSAYWIASHTTEEKALNVTLSSMGRNGLKTHGLHLNNHQVKGLEEELKILRTYNVLDMKNTTCQDLQIEVKVTGAVEYAWDANEDYEDYYDMPAADDPSVPLQPVTPLQLFEGRRSRRRREAPKVVEEQESRVQYTVCIWRNGKLGLSGMAIADITLLSGFHALRADLEKLTSLSDRYVSHFETDGPHVLLYFDSVPTTRECVGFGASQEVVVGLVQPSSAVLYDYYSPDHKCSVFYAAPTKSQLLATLCSGDVCQCAEGKCPRLLRSLERRVEDKDGYRMRFACYYPRVEYGFTVKVLREDGRAAFRLFESKITQVLHFRKDTMASIGQTRNFLSRASCRLRLEPNKEYLIMGMDGETSDNKGDPQYLLDSNTWIEEMPSEQMCKSTRHRAACFQLKDFLMEFSSRGCQVRQSATHPGFSGLSLGPESSA

Target ORF information:

RefSeq Version	XM_006523532.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523532.4

ORF Insert Sequence:

ATGCGGCTCC TCTGGGGGCT GGCCTGGGTG TTCAGCTTCT GTGCCTCATC CCTGCAGAAG 
CCCAGGTTGC TCCTGTTTTC CCCTTCTGTG GTTAATTTGG GGACCCCCCT GTCGGTGGGG 
GTACAGCTCC TGGATGCCCC TCCAGGACAG GAGGTAAAAG GATCAGTGTT CCTCAGAAAC 
CCAAAGGGTG GTTCCTGCTC CCCAAAGAAG GACTTTAAGC TGAGCTCGGG AGATGACTTT 
GTGCTGCTCA GCCTTGAGGT CCCACTGGAA GATGTGAGGA GCTGTGGCCT CTTTGACCTG 
CGCAGAGCCC CCCACATCCA GCTGGTAGCT CAGTCTCCGT GGCTAAGGAA CACAGCTTTC 
AAAGCCACAG AGACTCAGGG TGTCAACTTG CTCTTCTCTT CCCGACGAGG CCACATCTTT 
GTGCAGACCG ATCAGCCTAT CTATAATCCG GGGCAGCGGG TTCGTTATCG GGTCTTTGCA 
CTGGATCAAA AGATGCGCCC ATCCACTGAT TTCCTCACCA TCACAGTGGA GAACTCCCAT 
GGCCTCCGTG TACTCAAGAA GGAGATATTT ACTTCCACAT CCATCTTCCA AGATGCCTTC 
ACCATTCCAG ACATCTCAGA GCCTGGGACC TGGAAGATCT CAGCTAGGTT CTCAGATGGA 
CTGGAGTCCA ATAGGAGCAC CCACTTTGAA GTGAAGAAGT ATGTCCTTCC CAACTTCGAG 
GTGAAGATTA CTCCTTGGAA GCCATATATC CTGATGGTGC CCAGCAACAG TGATGAAATC 
CAATTAGACA TCCAGGCCAG GTACATCTAT GGGAAGCCCG TGCAGGGCGT GGCATACACA 
CGGTTTGCGC TCATGGATGA GCAAGGGAAG AGGACTTTCC TTCGGGGCCT AGAGACGCAG 
GCCAAGTTGG TGGAAGGCCG GACCCACATT TCCATCTCAA AGGACCAGTT CCAGGCTGCC 
CTGGATAAAA TCAATATTGG GGTCAGAGAC CTGGAGGGGC TGCGTCTCTA TGCTGCTACA 
GCTGTCATCG AGTCTCCAGG AGGAGAGATG GAGGAGGCAG AACTCACGTC CTGGCGCTTT 
GTATCATCTG CCTTCTCCTT GGATCTCAGC CGCACTAAGA GGCATCTTGT GCCTGGGGCC 
CACTTCCTGC TGCAGGCTCT GGTCCAAGAA ATGTCAGGCT CTGAAGCCTC CAACGTTCCT 
GTTAAAGTCT CTGCCACATT GGTGTCAGGC TCTGACTCCC AAGTCCTTGA CATTCAACAG 
AGCACCAATG GAATTGGCCA AGTCAGCATT TCCTTCCCCA TCCCACCAAC TGTCACAGAA 
CTTCGACTCT TGGTGTCTGC GGGCTCCCTC TACCCAGCCA TAGCCAGGCT CACCGTGCAA 
GCCCCACCTT CAAGAGGCAC TGGCTTTCTT TCTATTGAGC CACTAGACCC TCGGTCCCCT 
AGTGTGGGGG ACACCTTTAT CCTAAACCTT CAACCTGTGG GCATCCCTGC ACCTACCTTC 
TCTCATTACT ACTACATGAT CATCTCCAGA GGCCAGATCA TGGCTATGGG TCGGGAGCCC 
CGGAAGACTG TGACCTCCGT CTCTGTGTTG GTGGACCATC AGCTGGCTCC CTCGTTCTAC 
TTTGTGGCTT ACTTCTATCA CCAAGGACAC CCGGTGGCCA ACTCTCTGCT CATCAACATC 
CAATCCAGGG ACTGTGAGGG CAAGCTGCAA TTGAAGGTGG ATGGTGCCAA GGAGTATCGT 
AATGCGGACA TGATGAAGCT CCGAATTCAA ACTGACTCCA AAGCCCTGGT GGCACTGGGA 
GCTGTGGACA TGGCTCTGTA TGCTGTGGGT GGTCGGTCTC ACAAACCCCT CGACATGAGC 
AAGGTCTTTG AAGTAATCAA CAGCTACAAT GTTGGCTGTG GTCCTGGAGG TGGGGATGAT 
GCCCTTCAGG TGTTCCAGGA TGCTGGTCTG GCCTTTTCTG ATGGTGATCG ACTAACTCAA 
ACCAGAGAGG ACCTGAGCTG TCCCAAGGAG AAGAAAAGTC GGCAAAAGAG AAATGTTAAC 
TTCCAGAAGG CTGTCAGTGA GAAGTTGGGC CAGTATTCTT CTCCAGATGC CAAGCGCTGC 
TGCCAAGACG GGATGACGAA GCTGCCCATG AAGCGTACCT GTGAGCAGCG GGCTGCCCGT 
GTGCCTCAGC AGGCCTGCCG TGAGCCCTTC TTGTCCTGTT GCAAGTTTGC TGAGGACCTT 
CGCAGGAACC AGACCAGGAG CCAGGCACAC CTTGCCCGAA ACAACCACAA CATGCTGCAG 
GAGGAAGACT TGATAGATGA AGACGACATT CTTGTGCGCA CCTCCTTCCC AGAGAACTGG 
CTCTGGAGAG TGGAACCTGT AGACAGCTCC AAACTGTTGA CAGTGTGGCT TCCTGATTCT 
ATGACCACAT GGGAGATTCA TGGTGTGAGC CTGTCCAAAA GCAAAGGTCT GTGTGTAGCC 
AAGCCAACTC GTGTTCGAGT GTTCAGAAAA TTCCACCTGC ACCTGCGCCT GCCCATCTCC 
ATCCGCCGCT TTGAGCAGTT TGAATTACGG CCTGTTCTTT ACAACTATCT GAATGATGAT 
GTGGCTGTGA GTGTCCATGT GACCCCAGTT GAGGGGCTGT GCCTGGCTGG TGGTGGAATG 
ATGGCCCAGC AGGTGACAGT GCCAGCAGGT TCTGCCCGGC CTGTGGCCTT CTCTGTGGTA 
CCCACAGCTG CTGCCAACGT GCCCCTGAAG GTGGTGGCTC GAGGGGTTTT TGATTTAGGG 
GATGCTGTGT CTAAGATTCT CCAAATTGAG AAGGAAGGAG CCATCCACAG AGAAGAGTTA 
GTCTACAACC TCGACCCCCT AAATAACCTG GGTCGGACTT TGGAGATTCC TGGCAGCTCG 
GATCCCAACA TCGTCCCTGA CGGAGACTTC AGCAGCTTAG TCAGGGTTAC AGCCTCGGAA 
CCCTTGGAGA CTATGGGTTC TGAAGGTGCC TTGTCCCCAG GAGGTGTGGC CTCCCTCCTG 
AGGCTTCCCC AGGGCTGTGC AGAGCAAACC ATGATCTATT TGGCTCCTAC CCTGACTGCT 
TCCAACTACC TGGACAGGAC AGAACAGTGG AGCAAACTGT CCCCTGAAAC AAAGGACCAT 
GCTGTGGATC TGATCCAAAA AGGATACATG AGGATCCAGC AGTTTCGGAA GAATGATGGC 
TCCTTTGGGG CTTGGTTACA CCGGGACAGC AGCACCTGGC TGACTGCCTT CGTGCTGAAG 
ATTCTGAGTT TGGCCCAGGA ACAGGTGGGC AACTCCCCGG AGAAGCTGCA GGAGACGGCT 
AGCTGGCTGC TGGCCCAGCA GCTGGGTGAT GGCTCCTTCC ACGACCCATG TCCAGTCATC 
CACAGAGCAA TGCAGGGGGG CTTGGTGGGG TCTGACGAGA CAGTGGCACT GACCGCCTTT 
GTGGTCATTG CCCTTCACCA TGGGTTGGAC GTCTTCCAGG ATGACGATGC GAAGCAGCTG 
AAGAACAGAG TGGAAGCCTC CATCACCAAG GCAAACTCAT TCTTGGGGCA GAAGGCAAGT 
GCTGGGCTCC TGGGTGCCCA TGCCGCCGCC ATCACAGCCT ATGCCCTTAC GCTGACCAAG 
GCCTCGGAGG ACCTGCGGAA TGTTGCCCAC AACAGCCTGA TGGCCATGGC TGAGGAAACA 
GGGGAACACC TCTACTGGGG CTTAGTCCTT GGCTCTCAGG ACAAAGTTGT GTTGCGCCCC 
ACAGCCCCCC GTAGCCCTAC AGAACCTGTG CCCCAGGCCC CAGCCTTGTG GATCGAAACC 
ACAGCCTATG CCCTGCTCCA CCTGCTTCTG CGGGAGGGAA AGGGAAAAAT GGCTGACAAG 
GCTGCATCCT GGCTCACCCA CCAGGGAAGC TTCCATGGGG CATTCCGCAG TACCCAGGAC 
ACTGTGGTCA CCCTGGATGC CCTGTCTGCC TACTGGATCG CTTCGCACAC CACTGAGGAG 
AAAGCACTGA ACGTGACGCT CAGCTCCATG GGCCGCAATG GGCTTAAGAC CCACGGGCTA 
CACTTGAACA ACCACCAAGT CAAGGGCCTG GAGGAGGAGC TAAAGATCCT TCGTACCTAC 
AATGTCCTGG ACATGAAGAA CACCACATGC CAGGACCTTC AGATAGAAGT GAAGGTCACA 
GGCGCTGTGG AATACGCATG GGATGCCAAT GAAGACTACG AAGACTACTA TGACATGCCA 
GCCGCAGATG ACCCCAGCGT CCCCTTGCAG CCTGTCACGC CCCTGCAGCT TTTTGAGGGT 
CGTAGGAGCC GCCGCAGGAG GGAGGCCCCC AAGGTGGTCG AAGAGCAGGA GTCCAGAGTT 
CAGTACACTG TGTGTATCTG GCGAAATGGC AAGCTGGGGC TGTCTGGCAT GGCCATCGCA 
GACATCACCC TCCTAAGTGG ATTCCACGCC CTGAGGGCTG ACCTGGAGAA GCTGACCTCT 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACTGACGGGC CCCATGTCCT GTTGTACTTT 
GACTCGGTCC CTACCACCCG GGAGTGTGTG GGCTTCGGAG CCTCGCAGGA GGTGGTTGTG 
GGACTGGTGC AGCCATCCAG TGCTGTCCTG TATGACTACT ACAGCCCTGA TCACAAGTGC 
TCTGTGTTTT ATGCTGCACC CACCAAGAGC CAGCTCCTGG CCACACTGTG CTCTGGAGAT 
GTATGCCAGT GCGCTGAGGG GAAGTGCCCT CGACTGCTAA GGTCACTGGA GCGAAGGGTG 
GAGGACAAGG ACGGCTACCG GATGAGGTTC GCCTGCTATT ATCCCCGAGT GGAGTATGGC 
TTCACGGTTA AGGTTCTTCG AGAAGATGGC AGAGCTGCCT TCCGTCTCTT TGAGTCCAAG 
ATCACCCAAG TCCTGCATTT CAGAAAGGAC ACCATGGCCT CCATAGGTCA GACCCGCAAC 
TTCCTGAGCC GGGCCTCTTG CCGCCTTCGT TTGGAGCCTA ACAAAGAGTA CTTGATCATG 
GGGATGGACG GGGAAACCAG TGACAACAAG GGAGACCCCC AGTACTTGCT GGACTCAAAT 
ACCTGGATTG AGGAGATGCC TTCAGAACAA ATGTGCAAGA GCACCCGCCA TCGGGCAGCC 
TGTTTCCAGC TCAAAGACTT CCTGATGGAG TTCAGCAGCC GGGGGTGCCA GGTGCGGCAG 
TCTGCCACCC ACCCAGGATT TTCAGGCCTG AGCCTTGGAC CAGAAAGCTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C4b ( XM_030249459.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105319.1 Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X3, mRNA.

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CloneID	OMu87079
Clone ID Related Accession (Same CDS sequence)	XM_030249459.1
Accession Version	XM_030249459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	complement C4-B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCTCC TCTGGGGGCT GGCCTGGGTG TTCAGCTTCT GTGCCTCATC CCTGCAGAAG 
CCCAGGTTGC TCCTGTTTTC CCCTTCTGTG GTTAATTTGG GGACCCCCCT GTCGGTGGGG 
GTACAGCTCC TGGATGCCCC TCCAGGACAG GAGGTAAAAG GATCAGTGTT CCTCAGAAAC 
CCAAAGGGTG GTTCCTGCTC CCCAAAGAAG GACTTTAAGC TGAGCTCGGG AGATGACTTT 
GTGCTGCTCA GCCTTGAGGT CCCACTGGAA GATGTGAGGA GCTGTGGCCT CTTTGACCTG 
CGCAGAGCCC CCCACATCCA GCTGGTAGCT CAGTCTCCGT GGCTAAGGAA CACAGCTTTC 
AAAGCCACAG AGACTCAGGG TGTCAACTTG CTCTTCTCTT CCCGACGAGG CCACATCTTT 
GTGCAGACCG ATCAGCCTAT CTATAATCCG GGGCAGCGGG TTCGTTATCG GGTCTTTGCA 
CTGGATCAAA AGATGCGCCC ATCCACTGAT TTCCTCACCA TCACAGTGGA GAACTCCCAT 
GGCCTCCGTG TACTCAAGAA GGAGATATTT ACTTCCACAT CCATCTTCCA AGATGCCTTC 
ACCATTCCAG ACATCTCAGA GCCTGGGACC TGGAAGATCT CAGCTAGGTT CTCAGATGGA 
CTGGAGTCCA ATAGGAGCAC CCACTTTGAA GTGAAGAAGT ATGTCCTTCC CAACTTCGAG 
GTGAAGATTA CTCCTTGGAA GCCATATATC CTGATGGTGC CCAGCAACAG TGATGAAATC 
CAATTAGACA TCCAGGCCAG GTACATCTAT GGGAAGCCCG TGCAGGGCGT GGCATACACA 
CGGTTTGCGC TCATGGATGA GCAAGGGAAG AGGACTTTCC TTCGGGGCCT AGAGACGCAG 
GCCAAGTTGG TGGAAGGCCG GACCCACATT TCCATCTCAA AGGACCAGTT CCAGGCTGCC 
CTGGATAAAA TCAATATTGG GGTCAGAGAC CTGGAGGGGC TGCGTCTCTA TGCTGCTACA 
GCTGTCATCG AGTCTCCAGG AGGAGAGATG GAGGAGGCAG AACTCACGTC CTGGCGCTTT 
GTATCATCTG CCTTCTCCTT GGATCTCAGC CGCACTAAGA GGCATCTTGT GCCTGGGGCC 
CACTTCCTGC TGCAGGCTCT GGTCCAAGAA ATGTCAGGCT CTGAAGCCTC CAACGTTCCT 
GTTAAAGTCT CTGCCACATT GGTGTCAGGC TCTGACTCCC AAGTCCTTGA CATTCAACAG 
AGCACCAATG GAATTGGCCA AGTCAGCATT TCCTTCCCCA TCCCACCAAC TGTCACAGAA 
CTTCGACTCT TGGTGTCTGC GGGCTCCCTC TACCCAGCCA TAGCCAGGCT CACCGTGCAA 
GCCCCACCTT CAAGAGGCAC TGGCTTTCTT TCTATTGAGC CACTAGACCC TCGGTCCCCT 
AGTGTGGGGG ACACCTTTAT CCTAAACCTT CAACCTGTGG GCATCCCTGC ACCTACCTTC 
TCTCATTACT ACTACATGAT CATCTCCAGA GGCCAGATCA TGGCTATGGG TCGGGAGCCC 
CGGAAGACTG TGACCTCCGT CTCTGTGTTG GTGGACCATC AGCTGGCTCC CTCGTTCTAC 
TTTGTGGCTT ACTTCTATCA CCAAGGACAC CCGGTGGCCA ACTCTCTGCT CATCAACATC 
CAATCCAGGG ACTGTGAGGG CAAGCTGCAA TTGAAGGTGG ATGGTGCCAA GGAGTATCGT 
AATGCGGACA TGATGAAGCT CCGAATTCAA ACTGACTCCA AAGCCCTGGT GGCACTGGGA 
GCTGTGGACA TGGCTCTGTA TGCTGTGGGT GGTCGGTCTC ACAAACCCCT CGACATGAGC 
AAGGTCTTTG AAGTAATCAA CAGCTACAAT GTTGGCTGTG GTCCTGGAGG TGGGGATGAT 
GCCCTTCAGG TGTTCCAGGA TGCTGGTCTG GCCTTTTCTG ATGGTGATCG ACTAACTCAA 
ACCAGAGAGG ACCTGAGCTG TCCCAAGGAG AAGAAAAGTC GGCAAAAGAG AAATGTTAAC 
TTCCAGAAGG CTGTCAGTGA GAAGTTGGGC CAGTATTCTT CTCCAGATGC CAAGCGCTGC 
TGCCAAGACG GGATGACGAA GCTGCCCATG AAGCGTACCT GTGAGCAGCG GGCTGCCCGT 
GTGCCTCAGC AGGCCTGCCG TGAGCCCTTC TTGTCCTGTT GCAAGTTTGC TGAGGACCTT 
CGCAGGAACC AGACCAGGAG CCAGGCACAC CTTGCCCGAA ACAACCACAA CATGCTGCAG 
GAGGAAGACT TGATAGATGA AGACGACATT CTTGTGCGCA CCTCCTTCCC AGAGAACTGG 
CTCTGGAGAG TGGAACCTGT AGACAGCTCC AAACTGTTGA CAGTGTGGCT TCCTGATTCT 
ATGACCACAT GGGAGATTCA TGGTGTGAGC CTGTCCAAAA GCAAAGGTCT GTGTGTAGCC 
AAGCCAACTC GTGTTCGAGT GTTCAGAAAA TTCCACCTGC ACCTGCGCCT GCCCATCTCC 
ATCCGCCGCT TTGAGCAGTT TGAATTACGG CCTGTTCTTT ACAACTATCT GAATGATGAT 
GTGGCTGTGA GTGTCCATGT GACCCCAGTT GAGGGGCTGT GCCTGGCTGG TGGTGGAATG 
ATGGCCCAGC AGGTGACAGT GCCAGCAGGT TCTGCCCGGC CTGTGGCCTT CTCTGTGGTA 
CCCACAGCTG CTGCCAACGT GCCCCTGAAG GTGGTGGCTC GAGGGGTTTT TGATTTAGGG 
GATGCTGTGT CTAAGATTCT CCAAATTGAG AAGGAAGGAG CCATCCACAG AGAAGAGTTA 
GTCTACAACC TCGACCCCCT AAATAACCTG GGTCGGACTT TGGAGATTCC TGGCAGCTCG 
GATCCCAACA TCGTCCCTGA CGGAGACTTC AGCAGCTTAG TCAGGGTTAC AGCCTCGGAA 
CCCTTGGAGA CTATGGGTTC TGAAGGTGCC TTGTCCCCAG GAGGTGTGGC CTCCCTCCTG 
AGGCTTCCCC AGGGCTGTGC AGAGCAAACC ATGATCTATT TGGCTCCTAC CCTGACTGCT 
TCCAACTACC TGGACAGGAC AGAACAGTGG AGCAAACTGT CCCCTGAAAC AAAGGACCAT 
GCTGTGGATC TGATCCAAAA AGGATACATG AGGATCCAGC AGTTTCGGAA GAATGATGGC 
TCCTTTGGGG CTTGGTTACA CCGGGACAGC AGCACCTGGC TGACTGCCTT CGTGCTGAAG 
ATTCTGAGTT TGGCCCAGGA ACAGGTGGGC AACTCCCCGG AGAAGCTGCA GGAGACGGCT 
AGCTGGCTGC TGGCCCAGCA GCTGGGTGAT GGCTCCTTCC ACGACCCATG TCCAGTCATC 
CACAGAGCAA TGCAGGGGGG CTTGGTGGGG TCTGACGAGA CAGTGGCACT GACCGCCTTT 
GTGGTCATTG CCCTTCACCA TGGGTTGGAC GTCTTCCAGG ATGACGATGC GAAGCAGCTG 
AAGAACAGAG TGGAAGCCTC CATCACCAAG GCAAACTCAT TCTTGGGGCA GAAGGCAAGT 
GCTGGGCTCC TGGGTGCCCA TGCCGCCGCC ATCACAGCCT ATGCCCTTAC GCTGACCAAG 
GCCTCGGAGG ACCTGCGGAA TGTTGCCCAC AACAGCCTGA TGGCCATGGC TGAGGAAACA 
GGGGAACACC TCTACTGGGG CTTAGTCCTT GGCTCTCAGG ACAAAGTTGT GTTGCGCCCC 
ACAGCCCCCC GTAGCCCTAC AGAACCTGTG CCCCAGGCCC CAGCCTTGTG GATCGAAACC 
ACAGCCTATG CCCTGCTCCA CCTGCTTCTG CGGGAGGGAA AGGGAAAAAT GGCTGACAAG 
GCTGCATCCT GGCTCACCCA CCAGGGAAGC TTCCATGGGG CATTCCGCAG TACCCAGGAC 
ACTGTGGTCA CCCTGGATGC CCTGTCTGCC TACTGGATCG CTTCGCACAC CACTGAGGAG 
AAAGCACTGA ACGTGACGCT CAGCTCCATG GGCCGCAATG GGCTTAAGAC CCACGGGCTA 
CACTTGAACA ACCACCAAGT CAAGGGCCTG GAGGAGGAGC TAAAGATCCT TCGTACCTAC 
AATGTCCTGG ACATGAAGAA CACCACATGC CAGGACCTTC AGATAGAAGT GAAGGTCACA 
GGCGCTGTGG AATACGCATG GGATGCCAAT GAAGACTACG AAGACTACTA TGACATGCCA 
GCCGCAGATG ACCCCAGCGT CCCCTTGCAG CCTGTCACGC CCCTGCAGCT TTTTGAGGGT 
CGTAGGAGCC GCCGCAGGAG GGAGGCCCCC AAGGTGGTCG AAGAGCAGGA GTCCAGAGTT 
CAGTACACTG TGTGTATCTG GCGAAATGGC AAGCTGGGGC TGTCTGGCAT GGCCATCGCA 
GACATCACCC TCCTAAGTGG ATTCCACGCC CTGAGGGCTG ACCTGGAGAA GCTGACCTCT 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACTGACGGGC CCCATGTCCT GTTGTACTTT 
GACTCGGTCC CTACCACCCG GGAGTGTGTG GGCTTCGGAG CCTCGCAGGA GGTGGTTGTG 
GGACTGGTGC AGCCATCCAG TGCTGTCCTG TATGACTACT ACAGCCCTGA TCACAAGTGC 
TCTGTGTTTT ATGCTGCACC CACCAAGAGC CAGCTCCTGG CCACACTGTG CTCTGGAGAT 
GTATGCCAGT GCGCTGAGGG GAAGTGCCCT CGACTGCTAA GGTCACTGGA GCGAAGGGTG 
GAGGACAAGG ACGGCTACCG GATGAGGTTC GCCTGCTATT ATCCCCGAGT GGAGTATGGC 
TTCACGGTTA AGGTTCTTCG AGAAGATGGC AGAGCTGCCT TCCGTCTCTT TGAGTCCAAG 
ATCACCCAAG TCCTGCATTT CAGAAAGGAC ACCATGGCCT CCATAGGTCA GACCCGCAAC 
TTCCTGAGCC GGGCCTCTTG CCGCCTTCGT TTGGAGCCTA ACAAAGAGTA CTTGATCATG 
GGGATGGACG GGGAAACCAG TGACAACAAG GGAGACCCCC AGTACTTGCT GGACTCAAAT 
ACCTGGATTG AGGAGATGCC TTCAGAACAA ATGTGCAAGA GCACCCGCCA TCGGGCAGCC 
TGTTTCCAGC TCAAAGACTT CCTGATGGAG TTCAGCAGCC GGGGGTGCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105319.1
CDS	77..5233
Translation	MRLLWGLAWVFSFCASSLQKPRLLLFSPSVVNLGTPLSVGVQLLDAPPGQEVKGSVFLRNPKGGSCSPKKDFKLSSGDDFVLLSLEVPLEDVRSCGLFDLRRAPHIQLVAQSPWLRNTAFKATETQGVNLLFSSRRGHIFVQTDQPIYNPGQRVRYRVFALDQKMRPSTDFLTITVENSHGLRVLKKEIFTSTSIFQDAFTIPDISEPGTWKISARFSDGLESNRSTHFEVKKYVLPNFEVKITPWKPYILMVPSNSDEIQLDIQARYIYGKPVQGVAYTRFALMDEQGKRTFLRGLETQAKLVEGRTHISISKDQFQAALDKINIGVRDLEGLRLYAATAVIESPGGEMEEAELTSWRFVSSAFSLDLSRTKRHLVPGAHFLLQALVQEMSGSEASNVPVKVSATLVSGSDSQVLDIQQSTNGIGQVSISFPIPPTVTELRLLVSAGSLYPAIARLTVQAPPSRGTGFLSIEPLDPRSPSVGDTFILNLQPVGIPAPTFSHYYYMIISRGQIMAMGREPRKTVTSVSVLVDHQLAPSFYFVAYFYHQGHPVANSLLINIQSRDCEGKLQLKVDGAKEYRNADMMKLRIQTDSKALVALGAVDMALYAVGGRSHKPLDMSKVFEVINSYNVGCGPGGGDDALQVFQDAGLAFSDGDRLTQTREDLSCPKEKKSRQKRNVNFQKAVSEKLGQYSSPDAKRCCQDGMTKLPMKRTCEQRAARVPQQACREPFLSCCKFAEDLRRNQTRSQAHLARNNHNMLQEEDLIDEDDILVRTSFPENWLWRVEPVDSSKLLTVWLPDSMTTWEIHGVSLSKSKGLCVAKPTRVRVFRKFHLHLRLPISIRRFEQFELRPVLYNYLNDDVAVSVHVTPVEGLCLAGGGMMAQQVTVPAGSARPVAFSVVPTAAANVPLKVVARGVFDLGDAVSKILQIEKEGAIHREELVYNLDPLNNLGRTLEIPGSSDPNIVPDGDFSSLVRVTASEPLETMGSEGALSPGGVASLLRLPQGCAEQTMIYLAPTLTASNYLDRTEQWSKLSPETKDHAVDLIQKGYMRIQQFRKNDGSFGAWLHRDSSTWLTAFVLKILSLAQEQVGNSPEKLQETASWLLAQQLGDGSFHDPCPVIHRAMQGGLVGSDETVALTAFVVIALHHGLDVFQDDDAKQLKNRVEASITKANSFLGQKASAGLLGAHAAAITAYALTLTKASEDLRNVAHNSLMAMAEETGEHLYWGLVLGSQDKVVLRPTAPRSPTEPVPQAPALWIETTAYALLHLLLREGKGKMADKAASWLTHQGSFHGAFRSTQDTVVTLDALSAYWIASHTTEEKALNVTLSSMGRNGLKTHGLHLNNHQVKGLEEELKILRTYNVLDMKNTTCQDLQIEVKVTGAVEYAWDANEDYEDYYDMPAADDPSVPLQPVTPLQLFEGRRSRRRREAPKVVEEQESRVQYTVCIWRNGKLGLSGMAIADITLLSGFHALRADLEKLTSLSDRYVSHFETDGPHVLLYFDSVPTTRECVGFGASQEVVVGLVQPSSAVLYDYYSPDHKCSVFYAAPTKSQLLATLCSGDVCQCAEGKCPRLLRSLERRVEDKDGYRMRFACYYPRVEYGFTVKVLREDGRAAFRLFESKITQVLHFRKDTMASIGQTRNFLSRASCRLRLEPNKEYLIMGMDGETSDNKGDPQYLLDSNTWIEEMPSEQMCKSTRHRAACFQLKDFLMEFSSRGCQV

Target ORF information:

RefSeq Version	XM_030249459.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249459.1

ORF Insert Sequence:

ATGCGGCTCC TCTGGGGGCT GGCCTGGGTG TTCAGCTTCT GTGCCTCATC CCTGCAGAAG 
CCCAGGTTGC TCCTGTTTTC CCCTTCTGTG GTTAATTTGG GGACCCCCCT GTCGGTGGGG 
GTACAGCTCC TGGATGCCCC TCCAGGACAG GAGGTAAAAG GATCAGTGTT CCTCAGAAAC 
CCAAAGGGTG GTTCCTGCTC CCCAAAGAAG GACTTTAAGC TGAGCTCGGG AGATGACTTT 
GTGCTGCTCA GCCTTGAGGT CCCACTGGAA GATGTGAGGA GCTGTGGCCT CTTTGACCTG 
CGCAGAGCCC CCCACATCCA GCTGGTAGCT CAGTCTCCGT GGCTAAGGAA CACAGCTTTC 
AAAGCCACAG AGACTCAGGG TGTCAACTTG CTCTTCTCTT CCCGACGAGG CCACATCTTT 
GTGCAGACCG ATCAGCCTAT CTATAATCCG GGGCAGCGGG TTCGTTATCG GGTCTTTGCA 
CTGGATCAAA AGATGCGCCC ATCCACTGAT TTCCTCACCA TCACAGTGGA GAACTCCCAT 
GGCCTCCGTG TACTCAAGAA GGAGATATTT ACTTCCACAT CCATCTTCCA AGATGCCTTC 
ACCATTCCAG ACATCTCAGA GCCTGGGACC TGGAAGATCT CAGCTAGGTT CTCAGATGGA 
CTGGAGTCCA ATAGGAGCAC CCACTTTGAA GTGAAGAAGT ATGTCCTTCC CAACTTCGAG 
GTGAAGATTA CTCCTTGGAA GCCATATATC CTGATGGTGC CCAGCAACAG TGATGAAATC 
CAATTAGACA TCCAGGCCAG GTACATCTAT GGGAAGCCCG TGCAGGGCGT GGCATACACA 
CGGTTTGCGC TCATGGATGA GCAAGGGAAG AGGACTTTCC TTCGGGGCCT AGAGACGCAG 
GCCAAGTTGG TGGAAGGCCG GACCCACATT TCCATCTCAA AGGACCAGTT CCAGGCTGCC 
CTGGATAAAA TCAATATTGG GGTCAGAGAC CTGGAGGGGC TGCGTCTCTA TGCTGCTACA 
GCTGTCATCG AGTCTCCAGG AGGAGAGATG GAGGAGGCAG AACTCACGTC CTGGCGCTTT 
GTATCATCTG CCTTCTCCTT GGATCTCAGC CGCACTAAGA GGCATCTTGT GCCTGGGGCC 
CACTTCCTGC TGCAGGCTCT GGTCCAAGAA ATGTCAGGCT CTGAAGCCTC CAACGTTCCT 
GTTAAAGTCT CTGCCACATT GGTGTCAGGC TCTGACTCCC AAGTCCTTGA CATTCAACAG 
AGCACCAATG GAATTGGCCA AGTCAGCATT TCCTTCCCCA TCCCACCAAC TGTCACAGAA 
CTTCGACTCT TGGTGTCTGC GGGCTCCCTC TACCCAGCCA TAGCCAGGCT CACCGTGCAA 
GCCCCACCTT CAAGAGGCAC TGGCTTTCTT TCTATTGAGC CACTAGACCC TCGGTCCCCT 
AGTGTGGGGG ACACCTTTAT CCTAAACCTT CAACCTGTGG GCATCCCTGC ACCTACCTTC 
TCTCATTACT ACTACATGAT CATCTCCAGA GGCCAGATCA TGGCTATGGG TCGGGAGCCC 
CGGAAGACTG TGACCTCCGT CTCTGTGTTG GTGGACCATC AGCTGGCTCC CTCGTTCTAC 
TTTGTGGCTT ACTTCTATCA CCAAGGACAC CCGGTGGCCA ACTCTCTGCT CATCAACATC 
CAATCCAGGG ACTGTGAGGG CAAGCTGCAA TTGAAGGTGG ATGGTGCCAA GGAGTATCGT 
AATGCGGACA TGATGAAGCT CCGAATTCAA ACTGACTCCA AAGCCCTGGT GGCACTGGGA 
GCTGTGGACA TGGCTCTGTA TGCTGTGGGT GGTCGGTCTC ACAAACCCCT CGACATGAGC 
AAGGTCTTTG AAGTAATCAA CAGCTACAAT GTTGGCTGTG GTCCTGGAGG TGGGGATGAT 
GCCCTTCAGG TGTTCCAGGA TGCTGGTCTG GCCTTTTCTG ATGGTGATCG ACTAACTCAA 
ACCAGAGAGG ACCTGAGCTG TCCCAAGGAG AAGAAAAGTC GGCAAAAGAG AAATGTTAAC 
TTCCAGAAGG CTGTCAGTGA GAAGTTGGGC CAGTATTCTT CTCCAGATGC CAAGCGCTGC 
TGCCAAGACG GGATGACGAA GCTGCCCATG AAGCGTACCT GTGAGCAGCG GGCTGCCCGT 
GTGCCTCAGC AGGCCTGCCG TGAGCCCTTC TTGTCCTGTT GCAAGTTTGC TGAGGACCTT 
CGCAGGAACC AGACCAGGAG CCAGGCACAC CTTGCCCGAA ACAACCACAA CATGCTGCAG 
GAGGAAGACT TGATAGATGA AGACGACATT CTTGTGCGCA CCTCCTTCCC AGAGAACTGG 
CTCTGGAGAG TGGAACCTGT AGACAGCTCC AAACTGTTGA CAGTGTGGCT TCCTGATTCT 
ATGACCACAT GGGAGATTCA TGGTGTGAGC CTGTCCAAAA GCAAAGGTCT GTGTGTAGCC 
AAGCCAACTC GTGTTCGAGT GTTCAGAAAA TTCCACCTGC ACCTGCGCCT GCCCATCTCC 
ATCCGCCGCT TTGAGCAGTT TGAATTACGG CCTGTTCTTT ACAACTATCT GAATGATGAT 
GTGGCTGTGA GTGTCCATGT GACCCCAGTT GAGGGGCTGT GCCTGGCTGG TGGTGGAATG 
ATGGCCCAGC AGGTGACAGT GCCAGCAGGT TCTGCCCGGC CTGTGGCCTT CTCTGTGGTA 
CCCACAGCTG CTGCCAACGT GCCCCTGAAG GTGGTGGCTC GAGGGGTTTT TGATTTAGGG 
GATGCTGTGT CTAAGATTCT CCAAATTGAG AAGGAAGGAG CCATCCACAG AGAAGAGTTA 
GTCTACAACC TCGACCCCCT AAATAACCTG GGTCGGACTT TGGAGATTCC TGGCAGCTCG 
GATCCCAACA TCGTCCCTGA CGGAGACTTC AGCAGCTTAG TCAGGGTTAC AGCCTCGGAA 
CCCTTGGAGA CTATGGGTTC TGAAGGTGCC TTGTCCCCAG GAGGTGTGGC CTCCCTCCTG 
AGGCTTCCCC AGGGCTGTGC AGAGCAAACC ATGATCTATT TGGCTCCTAC CCTGACTGCT 
TCCAACTACC TGGACAGGAC AGAACAGTGG AGCAAACTGT CCCCTGAAAC AAAGGACCAT 
GCTGTGGATC TGATCCAAAA AGGATACATG AGGATCCAGC AGTTTCGGAA GAATGATGGC 
TCCTTTGGGG CTTGGTTACA CCGGGACAGC AGCACCTGGC TGACTGCCTT CGTGCTGAAG 
ATTCTGAGTT TGGCCCAGGA ACAGGTGGGC AACTCCCCGG AGAAGCTGCA GGAGACGGCT 
AGCTGGCTGC TGGCCCAGCA GCTGGGTGAT GGCTCCTTCC ACGACCCATG TCCAGTCATC 
CACAGAGCAA TGCAGGGGGG CTTGGTGGGG TCTGACGAGA CAGTGGCACT GACCGCCTTT 
GTGGTCATTG CCCTTCACCA TGGGTTGGAC GTCTTCCAGG ATGACGATGC GAAGCAGCTG 
AAGAACAGAG TGGAAGCCTC CATCACCAAG GCAAACTCAT TCTTGGGGCA GAAGGCAAGT 
GCTGGGCTCC TGGGTGCCCA TGCCGCCGCC ATCACAGCCT ATGCCCTTAC GCTGACCAAG 
GCCTCGGAGG ACCTGCGGAA TGTTGCCCAC AACAGCCTGA TGGCCATGGC TGAGGAAACA 
GGGGAACACC TCTACTGGGG CTTAGTCCTT GGCTCTCAGG ACAAAGTTGT GTTGCGCCCC 
ACAGCCCCCC GTAGCCCTAC AGAACCTGTG CCCCAGGCCC CAGCCTTGTG GATCGAAACC 
ACAGCCTATG CCCTGCTCCA CCTGCTTCTG CGGGAGGGAA AGGGAAAAAT GGCTGACAAG 
GCTGCATCCT GGCTCACCCA CCAGGGAAGC TTCCATGGGG CATTCCGCAG TACCCAGGAC 
ACTGTGGTCA CCCTGGATGC CCTGTCTGCC TACTGGATCG CTTCGCACAC CACTGAGGAG 
AAAGCACTGA ACGTGACGCT CAGCTCCATG GGCCGCAATG GGCTTAAGAC CCACGGGCTA 
CACTTGAACA ACCACCAAGT CAAGGGCCTG GAGGAGGAGC TAAAGATCCT TCGTACCTAC 
AATGTCCTGG ACATGAAGAA CACCACATGC CAGGACCTTC AGATAGAAGT GAAGGTCACA 
GGCGCTGTGG AATACGCATG GGATGCCAAT GAAGACTACG AAGACTACTA TGACATGCCA 
GCCGCAGATG ACCCCAGCGT CCCCTTGCAG CCTGTCACGC CCCTGCAGCT TTTTGAGGGT 
CGTAGGAGCC GCCGCAGGAG GGAGGCCCCC AAGGTGGTCG AAGAGCAGGA GTCCAGAGTT 
CAGTACACTG TGTGTATCTG GCGAAATGGC AAGCTGGGGC TGTCTGGCAT GGCCATCGCA 
GACATCACCC TCCTAAGTGG ATTCCACGCC CTGAGGGCTG ACCTGGAGAA GCTGACCTCT 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACTGACGGGC CCCATGTCCT GTTGTACTTT 
GACTCGGTCC CTACCACCCG GGAGTGTGTG GGCTTCGGAG CCTCGCAGGA GGTGGTTGTG 
GGACTGGTGC AGCCATCCAG TGCTGTCCTG TATGACTACT ACAGCCCTGA TCACAAGTGC 
TCTGTGTTTT ATGCTGCACC CACCAAGAGC CAGCTCCTGG CCACACTGTG CTCTGGAGAT 
GTATGCCAGT GCGCTGAGGG GAAGTGCCCT CGACTGCTAA GGTCACTGGA GCGAAGGGTG 
GAGGACAAGG ACGGCTACCG GATGAGGTTC GCCTGCTATT ATCCCCGAGT GGAGTATGGC 
TTCACGGTTA AGGTTCTTCG AGAAGATGGC AGAGCTGCCT TCCGTCTCTT TGAGTCCAAG 
ATCACCCAAG TCCTGCATTT CAGAAAGGAC ACCATGGCCT CCATAGGTCA GACCCGCAAC 
TTCCTGAGCC GGGCCTCTTG CCGCCTTCGT TTGGAGCCTA ACAAAGAGTA CTTGATCATG 
GGGATGGACG GGGAAACCAG TGACAACAAG GGAGACCCCC AGTACTTGCT GGACTCAAAT 
ACCTGGATTG AGGAGATGCC TTCAGAACAA ATGTGCAAGA GCACCCGCCA TCGGGCAGCC 
TGTTTCCAGC TCAAAGACTT CCTGATGGAG TTCAGCAGCC GGGGGTGCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C4b ( XM_006523531.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523594.1 Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X1, mRNA.

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CloneID	OMu53047
Clone ID Related Accession (Same CDS sequence)	XM_006523531.3 , XM_006523531.4
Accession Version	XM_006523531.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	complement C4-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523531.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCTCC TCTGGGGGCT GGCCTGGGTG TTCAGCTTCT GTGCCTCATC CCTGCAGAAG 
CCCAGGTTGC TCCTGTTTTC CCCTTCTGTG GTTAATTTGG GGACCCCCCT GTCGGTGGGG 
GTACAGCTCC TGGATGCCCC TCCAGGACAG GAGGTAAAAG GATCAGTGTT CCTCAGAAAC 
CCAAAGGGTG GTTCCTGCTC CCCAAAGAAG GACTTTAAGC TGAGCTCGGG AGATGACTTT 
GTGCTGCTCA GCCTTGAGGT CCCACTGGAA GATGTGAGGA GCTGTGGCCT CTTTGACCTG 
CGCAGAGCCC CCCACATCCA GCTGGTAGCT CAGTCTCCGT GGCTAAGGAA CACAGCTTTC 
AAAGCCACAG AGACTCAGGG TGTCAACTTG CTCTTCTCTT CCCGACGAGG CCACATCTTT 
GTGCAGACCG ATCAGCCTAT CTATAATCCG GGGCAGCGGG TTCGTTATCG GGTCTTTGCA 
CTGGATCAAA AGATGCGCCC ATCCACTGAT TTCCTCACCA TCACAGTGGA GAACTCCCAT 
GGCCTCCGTG TACTCAAGAA GGAGATATTT ACTTCCACAT CCATCTTCCA AGATGCCTTC 
ACCATTCCAG ACATCTCAGA GCCTGGGACC TGGAAGATCT CAGCTAGGTT CTCAGATGGA 
CTGGAGTCCA ATAGGAGCAC CCACTTTGAA GTGAAGAAGT ATGTCCTTCC CAACTTCGAG 
GTGAAGATTA CTCCTTGGAA GCCATATATC CTGATGGTGC CCAGCAACAG TGATGAAATC 
CAATTAGACA TCCAGGCCAG GTACATCTAT GGGAAGCCCG TGCAGGGCGT GGCATACACA 
CGGTTTGCGC TCATGGATGA GCAAGGGAAG AGGACTTTCC TTCGGGGCCT AGAGACGCAG 
GCCAAGTTGG TGGAAGGCCG GACCCACATT TCCATCTCAA AGGACCAGTT CCAGGCTGCC 
CTGGATAAAA TCAATATTGG GGTCAGAGAC CTGGAGGGGC TGCGTCTCTA TGCTGCTACA 
GCTGTCATCG AGTCTCCAGG AGGAGAGATG GAGGAGGCAG AACTCACGTC CTGGCGCTTT 
GTATCATCTG CCTTCTCCTT GGATCTCAGC CGCACTAAGA GGCATCTTGT GCCTGGGGCC 
CACTTCCTGC TGCAGGCTCT GGTCCAAGAA ATGTCAGGCT CTGAAGCCTC CAACGTTCCT 
GTTAAAGTCT CTGCCACATT GGTGTCAGGC TCTGACTCCC AAGTCCTTGA CATTCAACAG 
AGCACCAATG GAATTGGCCA AGTCAGCATT TCCTTCCCCA TCCCACCAAC TGTCACAGAA 
CTTCGACTCT TGGTGTCTGC GGGCTCCCTC TACCCAGCCA TAGCCAGGCT CACCGTGCAA 
GCCCCACCTT CAAGAGGCAC TGGCTTTCTT TCTATTGAGC CACTAGACCC TCGGTCCCCT 
AGTGTGGGGG ACACCTTTAT CCTAAACCTT CAACCTGTGG GCATCCCTGC ACCTACCTTC 
TCTCATTACT ACTACATGAT CATCTCCAGA GGCCAGATCA TGGCTATGGG TCGGGAGCCC 
CGGAAGACTG TGACCTCCGT CTCTGTGTTG GTGGACCATC AGCTGGCTCC CTCGTTCTAC 
TTTGTGGCTT ACTTCTATCA CCAAGGACAC CCGGTGGCCA ACTCTCTGCT CATCAACATC 
CAATCCAGGG ACTGTGAGGG CAAGCTGCAA TTGAAGGTGG ATGGTGCCAA GGAGTATCGT 
AATGCGGACA TGATGAAGCT CCGAATTCAA ACTGACTCCA AAGCCCTGGT GGCACTGGGA 
GCTGTGGACA TGGCTCTGTA TGCTGTGGGT GGTCGGTCTC ACAAACCCCT CGACATGAGC 
AAGGTCTTTG AAGTAATCAA CAGCTACAAT GTTGGCTGTG GTCCTGGAGG TGGGGATGAT 
GCCCTTCAGG TGTTCCAGGA TGCTGGTCTG GCCTTTTCTG ATGGTGATCG ACTAACTCAA 
ACCAGAGAGG ACCTGAGCTG TCCCAAGGAG AAGAAAAGTC GGCAAAAGAG AAATGTTAAC 
TTCCAGAAGG CTGTCAGTGA GAAGTTGGGC CAGTATTCTT CTCCAGATGC CAAGCGCTGC 
TGCCAAGACG GGATGACGAA GCTGCCCATG AAGCGTACCT GTGAGCAGCG GGCTGCCCGT 
GTGCCTCAGC AGGCCTGCCG TGAGCCCTTC TTGTCCTGTT GCAAGTTTGC TGAGGACCTT 
CGCAGGAACC AGACCAGGAG CCAGGCACAC CTTGCCCGAA ACAACCACAA CATGCTGCAG 
GAGGAAGACT TGATAGATGA AGACGACATT CTTGTGCGCA CCTCCTTCCC AGAGAACTGG 
CTCTGGAGAG TGGAACCTGT AGACAGCTCC AAACTGTTGA CAGTGTGGCT TCCTGATTCT 
ATGACCACAT GGGAGATTCA TGGTGTGAGC CTGTCCAAAA GCAAAGGTCT GTGTGTAGCC 
AAGCCAACTC GTGTTCGAGT GTTCAGAAAA TTCCACCTGC ACCTGCGCCT GCCCATCTCC 
ATCCGCCGCT TTGAGCAGTT TGAATTACGG CCTGTTCTTT ACAACTATCT GAATGATGAT 
GTGGCTGTGA GTGTCCATGT GACCCCAGTT GAGGGGCTGT GCCTGGCTGG TGGTGGAATG 
ATGGCCCAGC AGGTGACAGT GCCAGCAGGT TCTGCCCGGC CTGTGGCCTT CTCTGTGGTA 
CCCACAGCTG CTGCCAACGT GCCCCTGAAG GTGGTGGCTC GAGGGGTTTT TGATTTAGGG 
GATGCTGTGT CTAAGATTCT CCAAATTGAG AAGGAAGGAG CCATCCACAG AGAAGAGTTA 
GTCTACAACC TCGACCCCCT AAATAACCTG GGTCGGACTT TGGAGATTCC TGGCAGCTCG 
GATCCCAACA TCGTCCCTGA CGGAGACTTC AGCAGCTTAG TCAGGGTTAC AGCCTCGGAA 
CCCTTGGAGA CTATGGGTTC TGAAGGTGCC TTGTCCCCAG GAGGTGTGGC CTCCCTCCTG 
AGGCTTCCCC AGGGCTGTGC AGAGCAAACC ATGATCTATT TGGCTCCTAC CCTGACTGCT 
TCCAACTACC TGGACAGGAC AGAACAGTGG AGCAAACTGT CCCCTGAAAC AAAGGACCAT 
GCTGTGGATC TGATCCAAAA AGGATACATG AGGATCCAGC AGTTTCGGAA GAATGATGGC 
TCCTTTGGGG CTTGGTTACA CCGGGACAGC AGCACCTGGC TGACTGCCTT CGTGCTGAAG 
ATTCTGAGTT TGGCCCAGGA ACAGGTGGGC AACTCCCCGG AGAAGCTGCA GGAGACGGCT 
AGCTGGCTGC TGGCCCAGCA GCTGGGTGAT GGCTCCTTCC ACGACCCATG TCCAGTCATC 
CACAGAGCAA TGCAGGGGGG CTTGGTGGGG TCTGACGAGA CAGTGGCACT GACCGCCTTT 
GTGGTCATTG CCCTTCACCA TGGGTTGGAC GTCTTCCAGG ATGACGATGC GAAGCAGCTG 
AAGAACAGAG TGGAAGCCTC CATCACCAAG GCAAACTCAT TCTTGGGGCA GAAGGCAAGT 
GCTGGGCTCC TGGGTGCCCA TGCCGCCGCC ATCACAGCCT ATGCCCTTAC GCTGACCAAG 
GCCTCGGAGG ACCTGCGGAA TGTTGCCCAC AACAGCCTGA TGGCCATGGC TGAGGAAACA 
GGGGAACACC TCTACTGGGG CTTAGTCCTT GGCTCTCAGG ACAAAGTTGT GTTGCGCCCC 
ACAGCCCCCC GTAGCCCTAC AGAACCTGTG CCCCAGGCCC CAGCCTTGTG GATCGAAACC 
ACAGCCTATG CCCTGCTCCA CCTGCTTCTG CGGGAGGGAA AGGGAAAAAT GGCTGACAAG 
GCTGCATCCT GGCTCACCCA CCAGGGAAGC TTCCATGGGG CATTCCGCAG TACCCAGGAC 
ACTGTGGTCA CCCTGGATGC CCTGTCTGCC TACTGGATCG CTTCGCACAC CACTGAGGAG 
AAAGCACTGA ACGTGACGCT CAGCTCCATG GGCCGCAATG GGCTTAAGAC CCACGGGCTA 
CACTTGAACA ACCACCAAGT CAAGGGCCTG GAGGAGGAGC TAAAGTTCTC CCTGGGCAGC 
ACAATCAGTG TCAAGGTGGA AGGAAACAGC AAAGGCACCT TGAAGATCCT TCGTACCTAC 
AATGTCCTGG ACATGAAGAA CACCACATGC CAGGACCTTC AGATAGAAGT GAAGGTCACA 
GGCGCTGTGG AATACGCATG GGATGCCAAT GAAGACTACG AAGACTACTA TGACATGCCA 
GCCGCAGATG ACCCCAGCGT CCCCTTGCAG CCTGTCACGC CCCTGCAGCT TTTTGAGGGT 
CGTAGGAGCC GCCGCAGGAG GGAGGCCCCC AAGGTGGTCG AAGAGCAGGA GTCCAGAGTT 
CAGTACACTG TGTGTATCTG GCGAAATGGC AAGCTGGGGC TGTCTGGCAT GGCCATCGCA 
GACATCACCC TCCTAAGTGG ATTCCACGCC CTGAGGGCTG ACCTGGAGAA GCTGACCTCT 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACTGACGGGC CCCATGTCCT GTTGTACTTT 
GACTCGGTCC CTACCACCCG GGAGTGTGTG GGCTTCGGAG CCTCGCAGGA GGTGGTTGTG 
GGACTGGTGC AGCCATCCAG TGCTGTCCTG TATGACTACT ACAGCCCTGA TCACAAGTGC 
TCTGTGTTTT ATGCTGCACC CACCAAGAGC CAGCTCCTGG CCACACTGTG CTCTGGAGAT 
GTATGCCAGT GCGCTGAGGG GAAGTGCCCT CGACTGCTAA GGTCACTGGA GCGAAGGGTG 
GAGGACAAGG ACGGCTACCG GATGAGGTTC GCCTGCTATT ATCCCCGAGT GGAGTATGGC 
TTCACGGTTA AGGTTCTTCG AGAAGATGGC AGAGCTGCCT TCCGTCTCTT TGAGTCCAAG 
ATCACCCAAG TCCTGCATTT CAGAAAGGAC ACCATGGCCT CCATAGGTCA GACCCGCAAC 
TTCCTGAGCC GGGCCTCTTG CCGCCTTCGT TTGGAGCCTA ACAAAGAGTA CTTGATCATG 
GGGATGGACG GGGAAACCAG TGACAACAAG GGAGACCCCC AGTACTTGCT GGACTCAAAT 
ACCTGGATTG AGGAGATGCC TTCAGAACAA ATGTGCAAGA GCACCCGCCA TCGGGCAGCC 
TGTTTCCAGC TCAAAGACTT CCTGATGGAG TTCAGCAGCC GGGGGTGCCA GGTGCGGCAG 
TCTGCCACCC ACCCAGGATT TTCAGGCCTG AGCCTTGGAC CAGAAAGCTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523594.1
CDS	76..5352
Translation	MRLLWGLAWVFSFCASSLQKPRLLLFSPSVVNLGTPLSVGVQLLDAPPGQEVKGSVFLRNPKGGSCSPKKDFKLSSGDDFVLLSLEVPLEDVRSCGLFDLRRAPHIQLVAQSPWLRNTAFKATETQGVNLLFSSRRGHIFVQTDQPIYNPGQRVRYRVFALDQKMRPSTDFLTITVENSHGLRVLKKEIFTSTSIFQDAFTIPDISEPGTWKISARFSDGLESNRSTHFEVKKYVLPNFEVKITPWKPYILMVPSNSDEIQLDIQARYIYGKPVQGVAYTRFALMDEQGKRTFLRGLETQAKLVEGRTHISISKDQFQAALDKINIGVRDLEGLRLYAATAVIESPGGEMEEAELTSWRFVSSAFSLDLSRTKRHLVPGAHFLLQALVQEMSGSEASNVPVKVSATLVSGSDSQVLDIQQSTNGIGQVSISFPIPPTVTELRLLVSAGSLYPAIARLTVQAPPSRGTGFLSIEPLDPRSPSVGDTFILNLQPVGIPAPTFSHYYYMIISRGQIMAMGREPRKTVTSVSVLVDHQLAPSFYFVAYFYHQGHPVANSLLINIQSRDCEGKLQLKVDGAKEYRNADMMKLRIQTDSKALVALGAVDMALYAVGGRSHKPLDMSKVFEVINSYNVGCGPGGGDDALQVFQDAGLAFSDGDRLTQTREDLSCPKEKKSRQKRNVNFQKAVSEKLGQYSSPDAKRCCQDGMTKLPMKRTCEQRAARVPQQACREPFLSCCKFAEDLRRNQTRSQAHLARNNHNMLQEEDLIDEDDILVRTSFPENWLWRVEPVDSSKLLTVWLPDSMTTWEIHGVSLSKSKGLCVAKPTRVRVFRKFHLHLRLPISIRRFEQFELRPVLYNYLNDDVAVSVHVTPVEGLCLAGGGMMAQQVTVPAGSARPVAFSVVPTAAANVPLKVVARGVFDLGDAVSKILQIEKEGAIHREELVYNLDPLNNLGRTLEIPGSSDPNIVPDGDFSSLVRVTASEPLETMGSEGALSPGGVASLLRLPQGCAEQTMIYLAPTLTASNYLDRTEQWSKLSPETKDHAVDLIQKGYMRIQQFRKNDGSFGAWLHRDSSTWLTAFVLKILSLAQEQVGNSPEKLQETASWLLAQQLGDGSFHDPCPVIHRAMQGGLVGSDETVALTAFVVIALHHGLDVFQDDDAKQLKNRVEASITKANSFLGQKASAGLLGAHAAAITAYALTLTKASEDLRNVAHNSLMAMAEETGEHLYWGLVLGSQDKVVLRPTAPRSPTEPVPQAPALWIETTAYALLHLLLREGKGKMADKAASWLTHQGSFHGAFRSTQDTVVTLDALSAYWIASHTTEEKALNVTLSSMGRNGLKTHGLHLNNHQVKGLEEELKFSLGSTISVKVEGNSKGTLKILRTYNVLDMKNTTCQDLQIEVKVTGAVEYAWDANEDYEDYYDMPAADDPSVPLQPVTPLQLFEGRRSRRRREAPKVVEEQESRVQYTVCIWRNGKLGLSGMAIADITLLSGFHALRADLEKLTSLSDRYVSHFETDGPHVLLYFDSVPTTRECVGFGASQEVVVGLVQPSSAVLYDYYSPDHKCSVFYAAPTKSQLLATLCSGDVCQCAEGKCPRLLRSLERRVEDKDGYRMRFACYYPRVEYGFTVKVLREDGRAAFRLFESKITQVLHFRKDTMASIGQTRNFLSRASCRLRLEPNKEYLIMGMDGETSDNKGDPQYLLDSNTWIEEMPSEQMCKSTRHRAACFQLKDFLMEFSSRGCQVRQSATHPGFSGLSLGPESSA

Target ORF information:

RefSeq Version	XM_006523531.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523531.3

ORF Insert Sequence:

ATGCGGCTCC TCTGGGGGCT GGCCTGGGTG TTCAGCTTCT GTGCCTCATC CCTGCAGAAG 
CCCAGGTTGC TCCTGTTTTC CCCTTCTGTG GTTAATTTGG GGACCCCCCT GTCGGTGGGG 
GTACAGCTCC TGGATGCCCC TCCAGGACAG GAGGTAAAAG GATCAGTGTT CCTCAGAAAC 
CCAAAGGGTG GTTCCTGCTC CCCAAAGAAG GACTTTAAGC TGAGCTCGGG AGATGACTTT 
GTGCTGCTCA GCCTTGAGGT CCCACTGGAA GATGTGAGGA GCTGTGGCCT CTTTGACCTG 
CGCAGAGCCC CCCACATCCA GCTGGTAGCT CAGTCTCCGT GGCTAAGGAA CACAGCTTTC 
AAAGCCACAG AGACTCAGGG TGTCAACTTG CTCTTCTCTT CCCGACGAGG CCACATCTTT 
GTGCAGACCG ATCAGCCTAT CTATAATCCG GGGCAGCGGG TTCGTTATCG GGTCTTTGCA 
CTGGATCAAA AGATGCGCCC ATCCACTGAT TTCCTCACCA TCACAGTGGA GAACTCCCAT 
GGCCTCCGTG TACTCAAGAA GGAGATATTT ACTTCCACAT CCATCTTCCA AGATGCCTTC 
ACCATTCCAG ACATCTCAGA GCCTGGGACC TGGAAGATCT CAGCTAGGTT CTCAGATGGA 
CTGGAGTCCA ATAGGAGCAC CCACTTTGAA GTGAAGAAGT ATGTCCTTCC CAACTTCGAG 
GTGAAGATTA CTCCTTGGAA GCCATATATC CTGATGGTGC CCAGCAACAG TGATGAAATC 
CAATTAGACA TCCAGGCCAG GTACATCTAT GGGAAGCCCG TGCAGGGCGT GGCATACACA 
CGGTTTGCGC TCATGGATGA GCAAGGGAAG AGGACTTTCC TTCGGGGCCT AGAGACGCAG 
GCCAAGTTGG TGGAAGGCCG GACCCACATT TCCATCTCAA AGGACCAGTT CCAGGCTGCC 
CTGGATAAAA TCAATATTGG GGTCAGAGAC CTGGAGGGGC TGCGTCTCTA TGCTGCTACA 
GCTGTCATCG AGTCTCCAGG AGGAGAGATG GAGGAGGCAG AACTCACGTC CTGGCGCTTT 
GTATCATCTG CCTTCTCCTT GGATCTCAGC CGCACTAAGA GGCATCTTGT GCCTGGGGCC 
CACTTCCTGC TGCAGGCTCT GGTCCAAGAA ATGTCAGGCT CTGAAGCCTC CAACGTTCCT 
GTTAAAGTCT CTGCCACATT GGTGTCAGGC TCTGACTCCC AAGTCCTTGA CATTCAACAG 
AGCACCAATG GAATTGGCCA AGTCAGCATT TCCTTCCCCA TCCCACCAAC TGTCACAGAA 
CTTCGACTCT TGGTGTCTGC GGGCTCCCTC TACCCAGCCA TAGCCAGGCT CACCGTGCAA 
GCCCCACCTT CAAGAGGCAC TGGCTTTCTT TCTATTGAGC CACTAGACCC TCGGTCCCCT 
AGTGTGGGGG ACACCTTTAT CCTAAACCTT CAACCTGTGG GCATCCCTGC ACCTACCTTC 
TCTCATTACT ACTACATGAT CATCTCCAGA GGCCAGATCA TGGCTATGGG TCGGGAGCCC 
CGGAAGACTG TGACCTCCGT CTCTGTGTTG GTGGACCATC AGCTGGCTCC CTCGTTCTAC 
TTTGTGGCTT ACTTCTATCA CCAAGGACAC CCGGTGGCCA ACTCTCTGCT CATCAACATC 
CAATCCAGGG ACTGTGAGGG CAAGCTGCAA TTGAAGGTGG ATGGTGCCAA GGAGTATCGT 
AATGCGGACA TGATGAAGCT CCGAATTCAA ACTGACTCCA AAGCCCTGGT GGCACTGGGA 
GCTGTGGACA TGGCTCTGTA TGCTGTGGGT GGTCGGTCTC ACAAACCCCT CGACATGAGC 
AAGGTCTTTG AAGTAATCAA CAGCTACAAT GTTGGCTGTG GTCCTGGAGG TGGGGATGAT 
GCCCTTCAGG TGTTCCAGGA TGCTGGTCTG GCCTTTTCTG ATGGTGATCG ACTAACTCAA 
ACCAGAGAGG ACCTGAGCTG TCCCAAGGAG AAGAAAAGTC GGCAAAAGAG AAATGTTAAC 
TTCCAGAAGG CTGTCAGTGA GAAGTTGGGC CAGTATTCTT CTCCAGATGC CAAGCGCTGC 
TGCCAAGACG GGATGACGAA GCTGCCCATG AAGCGTACCT GTGAGCAGCG GGCTGCCCGT 
GTGCCTCAGC AGGCCTGCCG TGAGCCCTTC TTGTCCTGTT GCAAGTTTGC TGAGGACCTT 
CGCAGGAACC AGACCAGGAG CCAGGCACAC CTTGCCCGAA ACAACCACAA CATGCTGCAG 
GAGGAAGACT TGATAGATGA AGACGACATT CTTGTGCGCA CCTCCTTCCC AGAGAACTGG 
CTCTGGAGAG TGGAACCTGT AGACAGCTCC AAACTGTTGA CAGTGTGGCT TCCTGATTCT 
ATGACCACAT GGGAGATTCA TGGTGTGAGC CTGTCCAAAA GCAAAGGTCT GTGTGTAGCC 
AAGCCAACTC GTGTTCGAGT GTTCAGAAAA TTCCACCTGC ACCTGCGCCT GCCCATCTCC 
ATCCGCCGCT TTGAGCAGTT TGAATTACGG CCTGTTCTTT ACAACTATCT GAATGATGAT 
GTGGCTGTGA GTGTCCATGT GACCCCAGTT GAGGGGCTGT GCCTGGCTGG TGGTGGAATG 
ATGGCCCAGC AGGTGACAGT GCCAGCAGGT TCTGCCCGGC CTGTGGCCTT CTCTGTGGTA 
CCCACAGCTG CTGCCAACGT GCCCCTGAAG GTGGTGGCTC GAGGGGTTTT TGATTTAGGG 
GATGCTGTGT CTAAGATTCT CCAAATTGAG AAGGAAGGAG CCATCCACAG AGAAGAGTTA 
GTCTACAACC TCGACCCCCT AAATAACCTG GGTCGGACTT TGGAGATTCC TGGCAGCTCG 
GATCCCAACA TCGTCCCTGA CGGAGACTTC AGCAGCTTAG TCAGGGTTAC AGCCTCGGAA 
CCCTTGGAGA CTATGGGTTC TGAAGGTGCC TTGTCCCCAG GAGGTGTGGC CTCCCTCCTG 
AGGCTTCCCC AGGGCTGTGC AGAGCAAACC ATGATCTATT TGGCTCCTAC CCTGACTGCT 
TCCAACTACC TGGACAGGAC AGAACAGTGG AGCAAACTGT CCCCTGAAAC AAAGGACCAT 
GCTGTGGATC TGATCCAAAA AGGATACATG AGGATCCAGC AGTTTCGGAA GAATGATGGC 
TCCTTTGGGG CTTGGTTACA CCGGGACAGC AGCACCTGGC TGACTGCCTT CGTGCTGAAG 
ATTCTGAGTT TGGCCCAGGA ACAGGTGGGC AACTCCCCGG AGAAGCTGCA GGAGACGGCT 
AGCTGGCTGC TGGCCCAGCA GCTGGGTGAT GGCTCCTTCC ACGACCCATG TCCAGTCATC 
CACAGAGCAA TGCAGGGGGG CTTGGTGGGG TCTGACGAGA CAGTGGCACT GACCGCCTTT 
GTGGTCATTG CCCTTCACCA TGGGTTGGAC GTCTTCCAGG ATGACGATGC GAAGCAGCTG 
AAGAACAGAG TGGAAGCCTC CATCACCAAG GCAAACTCAT TCTTGGGGCA GAAGGCAAGT 
GCTGGGCTCC TGGGTGCCCA TGCCGCCGCC ATCACAGCCT ATGCCCTTAC GCTGACCAAG 
GCCTCGGAGG ACCTGCGGAA TGTTGCCCAC AACAGCCTGA TGGCCATGGC TGAGGAAACA 
GGGGAACACC TCTACTGGGG CTTAGTCCTT GGCTCTCAGG ACAAAGTTGT GTTGCGCCCC 
ACAGCCCCCC GTAGCCCTAC AGAACCTGTG CCCCAGGCCC CAGCCTTGTG GATCGAAACC 
ACAGCCTATG CCCTGCTCCA CCTGCTTCTG CGGGAGGGAA AGGGAAAAAT GGCTGACAAG 
GCTGCATCCT GGCTCACCCA CCAGGGAAGC TTCCATGGGG CATTCCGCAG TACCCAGGAC 
ACTGTGGTCA CCCTGGATGC CCTGTCTGCC TACTGGATCG CTTCGCACAC CACTGAGGAG 
AAAGCACTGA ACGTGACGCT CAGCTCCATG GGCCGCAATG GGCTTAAGAC CCACGGGCTA 
CACTTGAACA ACCACCAAGT CAAGGGCCTG GAGGAGGAGC TAAAGTTCTC CCTGGGCAGC 
ACAATCAGTG TCAAGGTGGA AGGAAACAGC AAAGGCACCT TGAAGATCCT TCGTACCTAC 
AATGTCCTGG ACATGAAGAA CACCACATGC CAGGACCTTC AGATAGAAGT GAAGGTCACA 
GGCGCTGTGG AATACGCATG GGATGCCAAT GAAGACTACG AAGACTACTA TGACATGCCA 
GCCGCAGATG ACCCCAGCGT CCCCTTGCAG CCTGTCACGC CCCTGCAGCT TTTTGAGGGT 
CGTAGGAGCC GCCGCAGGAG GGAGGCCCCC AAGGTGGTCG AAGAGCAGGA GTCCAGAGTT 
CAGTACACTG TGTGTATCTG GCGAAATGGC AAGCTGGGGC TGTCTGGCAT GGCCATCGCA 
GACATCACCC TCCTAAGTGG ATTCCACGCC CTGAGGGCTG ACCTGGAGAA GCTGACCTCT 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACTGACGGGC CCCATGTCCT GTTGTACTTT 
GACTCGGTCC CTACCACCCG GGAGTGTGTG GGCTTCGGAG CCTCGCAGGA GGTGGTTGTG 
GGACTGGTGC AGCCATCCAG TGCTGTCCTG TATGACTACT ACAGCCCTGA TCACAAGTGC 
TCTGTGTTTT ATGCTGCACC CACCAAGAGC CAGCTCCTGG CCACACTGTG CTCTGGAGAT 
GTATGCCAGT GCGCTGAGGG GAAGTGCCCT CGACTGCTAA GGTCACTGGA GCGAAGGGTG 
GAGGACAAGG ACGGCTACCG GATGAGGTTC GCCTGCTATT ATCCCCGAGT GGAGTATGGC 
TTCACGGTTA AGGTTCTTCG AGAAGATGGC AGAGCTGCCT TCCGTCTCTT TGAGTCCAAG 
ATCACCCAAG TCCTGCATTT CAGAAAGGAC ACCATGGCCT CCATAGGTCA GACCCGCAAC 
TTCCTGAGCC GGGCCTCTTG CCGCCTTCGT TTGGAGCCTA ACAAAGAGTA CTTGATCATG 
GGGATGGACG GGGAAACCAG TGACAACAAG GGAGACCCCC AGTACTTGCT GGACTCAAAT 
ACCTGGATTG AGGAGATGCC TTCAGAACAA ATGTGCAAGA GCACCCGCCA TCGGGCAGCC 
TGTTTCCAGC TCAAAGACTT CCTGATGGAG TTCAGCAGCC GGGGGTGCCA GGTGCGGCAG 
TCTGCCACCC ACCCAGGATT TTCAGGCCTG AGCCTTGGAC CAGAAAGCTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C4b ( XM_006523532.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523595.1 Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X2, mRNA.

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CloneID	OMu53048
Clone ID Related Accession (Same CDS sequence)	XM_006523532.4 , XM_006523532.3
Accession Version	XM_006523532.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	complement C4-B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523532.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCTCC TCTGGGGGCT GGCCTGGGTG TTCAGCTTCT GTGCCTCATC CCTGCAGAAG 
CCCAGGTTGC TCCTGTTTTC CCCTTCTGTG GTTAATTTGG GGACCCCCCT GTCGGTGGGG 
GTACAGCTCC TGGATGCCCC TCCAGGACAG GAGGTAAAAG GATCAGTGTT CCTCAGAAAC 
CCAAAGGGTG GTTCCTGCTC CCCAAAGAAG GACTTTAAGC TGAGCTCGGG AGATGACTTT 
GTGCTGCTCA GCCTTGAGGT CCCACTGGAA GATGTGAGGA GCTGTGGCCT CTTTGACCTG 
CGCAGAGCCC CCCACATCCA GCTGGTAGCT CAGTCTCCGT GGCTAAGGAA CACAGCTTTC 
AAAGCCACAG AGACTCAGGG TGTCAACTTG CTCTTCTCTT CCCGACGAGG CCACATCTTT 
GTGCAGACCG ATCAGCCTAT CTATAATCCG GGGCAGCGGG TTCGTTATCG GGTCTTTGCA 
CTGGATCAAA AGATGCGCCC ATCCACTGAT TTCCTCACCA TCACAGTGGA GAACTCCCAT 
GGCCTCCGTG TACTCAAGAA GGAGATATTT ACTTCCACAT CCATCTTCCA AGATGCCTTC 
ACCATTCCAG ACATCTCAGA GCCTGGGACC TGGAAGATCT CAGCTAGGTT CTCAGATGGA 
CTGGAGTCCA ATAGGAGCAC CCACTTTGAA GTGAAGAAGT ATGTCCTTCC CAACTTCGAG 
GTGAAGATTA CTCCTTGGAA GCCATATATC CTGATGGTGC CCAGCAACAG TGATGAAATC 
CAATTAGACA TCCAGGCCAG GTACATCTAT GGGAAGCCCG TGCAGGGCGT GGCATACACA 
CGGTTTGCGC TCATGGATGA GCAAGGGAAG AGGACTTTCC TTCGGGGCCT AGAGACGCAG 
GCCAAGTTGG TGGAAGGCCG GACCCACATT TCCATCTCAA AGGACCAGTT CCAGGCTGCC 
CTGGATAAAA TCAATATTGG GGTCAGAGAC CTGGAGGGGC TGCGTCTCTA TGCTGCTACA 
GCTGTCATCG AGTCTCCAGG AGGAGAGATG GAGGAGGCAG AACTCACGTC CTGGCGCTTT 
GTATCATCTG CCTTCTCCTT GGATCTCAGC CGCACTAAGA GGCATCTTGT GCCTGGGGCC 
CACTTCCTGC TGCAGGCTCT GGTCCAAGAA ATGTCAGGCT CTGAAGCCTC CAACGTTCCT 
GTTAAAGTCT CTGCCACATT GGTGTCAGGC TCTGACTCCC AAGTCCTTGA CATTCAACAG 
AGCACCAATG GAATTGGCCA AGTCAGCATT TCCTTCCCCA TCCCACCAAC TGTCACAGAA 
CTTCGACTCT TGGTGTCTGC GGGCTCCCTC TACCCAGCCA TAGCCAGGCT CACCGTGCAA 
GCCCCACCTT CAAGAGGCAC TGGCTTTCTT TCTATTGAGC CACTAGACCC TCGGTCCCCT 
AGTGTGGGGG ACACCTTTAT CCTAAACCTT CAACCTGTGG GCATCCCTGC ACCTACCTTC 
TCTCATTACT ACTACATGAT CATCTCCAGA GGCCAGATCA TGGCTATGGG TCGGGAGCCC 
CGGAAGACTG TGACCTCCGT CTCTGTGTTG GTGGACCATC AGCTGGCTCC CTCGTTCTAC 
TTTGTGGCTT ACTTCTATCA CCAAGGACAC CCGGTGGCCA ACTCTCTGCT CATCAACATC 
CAATCCAGGG ACTGTGAGGG CAAGCTGCAA TTGAAGGTGG ATGGTGCCAA GGAGTATCGT 
AATGCGGACA TGATGAAGCT CCGAATTCAA ACTGACTCCA AAGCCCTGGT GGCACTGGGA 
GCTGTGGACA TGGCTCTGTA TGCTGTGGGT GGTCGGTCTC ACAAACCCCT CGACATGAGC 
AAGGTCTTTG AAGTAATCAA CAGCTACAAT GTTGGCTGTG GTCCTGGAGG TGGGGATGAT 
GCCCTTCAGG TGTTCCAGGA TGCTGGTCTG GCCTTTTCTG ATGGTGATCG ACTAACTCAA 
ACCAGAGAGG ACCTGAGCTG TCCCAAGGAG AAGAAAAGTC GGCAAAAGAG AAATGTTAAC 
TTCCAGAAGG CTGTCAGTGA GAAGTTGGGC CAGTATTCTT CTCCAGATGC CAAGCGCTGC 
TGCCAAGACG GGATGACGAA GCTGCCCATG AAGCGTACCT GTGAGCAGCG GGCTGCCCGT 
GTGCCTCAGC AGGCCTGCCG TGAGCCCTTC TTGTCCTGTT GCAAGTTTGC TGAGGACCTT 
CGCAGGAACC AGACCAGGAG CCAGGCACAC CTTGCCCGAA ACAACCACAA CATGCTGCAG 
GAGGAAGACT TGATAGATGA AGACGACATT CTTGTGCGCA CCTCCTTCCC AGAGAACTGG 
CTCTGGAGAG TGGAACCTGT AGACAGCTCC AAACTGTTGA CAGTGTGGCT TCCTGATTCT 
ATGACCACAT GGGAGATTCA TGGTGTGAGC CTGTCCAAAA GCAAAGGTCT GTGTGTAGCC 
AAGCCAACTC GTGTTCGAGT GTTCAGAAAA TTCCACCTGC ACCTGCGCCT GCCCATCTCC 
ATCCGCCGCT TTGAGCAGTT TGAATTACGG CCTGTTCTTT ACAACTATCT GAATGATGAT 
GTGGCTGTGA GTGTCCATGT GACCCCAGTT GAGGGGCTGT GCCTGGCTGG TGGTGGAATG 
ATGGCCCAGC AGGTGACAGT GCCAGCAGGT TCTGCCCGGC CTGTGGCCTT CTCTGTGGTA 
CCCACAGCTG CTGCCAACGT GCCCCTGAAG GTGGTGGCTC GAGGGGTTTT TGATTTAGGG 
GATGCTGTGT CTAAGATTCT CCAAATTGAG AAGGAAGGAG CCATCCACAG AGAAGAGTTA 
GTCTACAACC TCGACCCCCT AAATAACCTG GGTCGGACTT TGGAGATTCC TGGCAGCTCG 
GATCCCAACA TCGTCCCTGA CGGAGACTTC AGCAGCTTAG TCAGGGTTAC AGCCTCGGAA 
CCCTTGGAGA CTATGGGTTC TGAAGGTGCC TTGTCCCCAG GAGGTGTGGC CTCCCTCCTG 
AGGCTTCCCC AGGGCTGTGC AGAGCAAACC ATGATCTATT TGGCTCCTAC CCTGACTGCT 
TCCAACTACC TGGACAGGAC AGAACAGTGG AGCAAACTGT CCCCTGAAAC AAAGGACCAT 
GCTGTGGATC TGATCCAAAA AGGATACATG AGGATCCAGC AGTTTCGGAA GAATGATGGC 
TCCTTTGGGG CTTGGTTACA CCGGGACAGC AGCACCTGGC TGACTGCCTT CGTGCTGAAG 
ATTCTGAGTT TGGCCCAGGA ACAGGTGGGC AACTCCCCGG AGAAGCTGCA GGAGACGGCT 
AGCTGGCTGC TGGCCCAGCA GCTGGGTGAT GGCTCCTTCC ACGACCCATG TCCAGTCATC 
CACAGAGCAA TGCAGGGGGG CTTGGTGGGG TCTGACGAGA CAGTGGCACT GACCGCCTTT 
GTGGTCATTG CCCTTCACCA TGGGTTGGAC GTCTTCCAGG ATGACGATGC GAAGCAGCTG 
AAGAACAGAG TGGAAGCCTC CATCACCAAG GCAAACTCAT TCTTGGGGCA GAAGGCAAGT 
GCTGGGCTCC TGGGTGCCCA TGCCGCCGCC ATCACAGCCT ATGCCCTTAC GCTGACCAAG 
GCCTCGGAGG ACCTGCGGAA TGTTGCCCAC AACAGCCTGA TGGCCATGGC TGAGGAAACA 
GGGGAACACC TCTACTGGGG CTTAGTCCTT GGCTCTCAGG ACAAAGTTGT GTTGCGCCCC 
ACAGCCCCCC GTAGCCCTAC AGAACCTGTG CCCCAGGCCC CAGCCTTGTG GATCGAAACC 
ACAGCCTATG CCCTGCTCCA CCTGCTTCTG CGGGAGGGAA AGGGAAAAAT GGCTGACAAG 
GCTGCATCCT GGCTCACCCA CCAGGGAAGC TTCCATGGGG CATTCCGCAG TACCCAGGAC 
ACTGTGGTCA CCCTGGATGC CCTGTCTGCC TACTGGATCG CTTCGCACAC CACTGAGGAG 
AAAGCACTGA ACGTGACGCT CAGCTCCATG GGCCGCAATG GGCTTAAGAC CCACGGGCTA 
CACTTGAACA ACCACCAAGT CAAGGGCCTG GAGGAGGAGC TAAAGATCCT TCGTACCTAC 
AATGTCCTGG ACATGAAGAA CACCACATGC CAGGACCTTC AGATAGAAGT GAAGGTCACA 
GGCGCTGTGG AATACGCATG GGATGCCAAT GAAGACTACG AAGACTACTA TGACATGCCA 
GCCGCAGATG ACCCCAGCGT CCCCTTGCAG CCTGTCACGC CCCTGCAGCT TTTTGAGGGT 
CGTAGGAGCC GCCGCAGGAG GGAGGCCCCC AAGGTGGTCG AAGAGCAGGA GTCCAGAGTT 
CAGTACACTG TGTGTATCTG GCGAAATGGC AAGCTGGGGC TGTCTGGCAT GGCCATCGCA 
GACATCACCC TCCTAAGTGG ATTCCACGCC CTGAGGGCTG ACCTGGAGAA GCTGACCTCT 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACTGACGGGC CCCATGTCCT GTTGTACTTT 
GACTCGGTCC CTACCACCCG GGAGTGTGTG GGCTTCGGAG CCTCGCAGGA GGTGGTTGTG 
GGACTGGTGC AGCCATCCAG TGCTGTCCTG TATGACTACT ACAGCCCTGA TCACAAGTGC 
TCTGTGTTTT ATGCTGCACC CACCAAGAGC CAGCTCCTGG CCACACTGTG CTCTGGAGAT 
GTATGCCAGT GCGCTGAGGG GAAGTGCCCT CGACTGCTAA GGTCACTGGA GCGAAGGGTG 
GAGGACAAGG ACGGCTACCG GATGAGGTTC GCCTGCTATT ATCCCCGAGT GGAGTATGGC 
TTCACGGTTA AGGTTCTTCG AGAAGATGGC AGAGCTGCCT TCCGTCTCTT TGAGTCCAAG 
ATCACCCAAG TCCTGCATTT CAGAAAGGAC ACCATGGCCT CCATAGGTCA GACCCGCAAC 
TTCCTGAGCC GGGCCTCTTG CCGCCTTCGT TTGGAGCCTA ACAAAGAGTA CTTGATCATG 
GGGATGGACG GGGAAACCAG TGACAACAAG GGAGACCCCC AGTACTTGCT GGACTCAAAT 
ACCTGGATTG AGGAGATGCC TTCAGAACAA ATGTGCAAGA GCACCCGCCA TCGGGCAGCC 
TGTTTCCAGC TCAAAGACTT CCTGATGGAG TTCAGCAGCC GGGGGTGCCA GGTGCGGCAG 
TCTGCCACCC ACCCAGGATT TTCAGGCCTG AGCCTTGGAC CAGAAAGCTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523595.1
CDS	76..5292
Translation	MRLLWGLAWVFSFCASSLQKPRLLLFSPSVVNLGTPLSVGVQLLDAPPGQEVKGSVFLRNPKGGSCSPKKDFKLSSGDDFVLLSLEVPLEDVRSCGLFDLRRAPHIQLVAQSPWLRNTAFKATETQGVNLLFSSRRGHIFVQTDQPIYNPGQRVRYRVFALDQKMRPSTDFLTITVENSHGLRVLKKEIFTSTSIFQDAFTIPDISEPGTWKISARFSDGLESNRSTHFEVKKYVLPNFEVKITPWKPYILMVPSNSDEIQLDIQARYIYGKPVQGVAYTRFALMDEQGKRTFLRGLETQAKLVEGRTHISISKDQFQAALDKINIGVRDLEGLRLYAATAVIESPGGEMEEAELTSWRFVSSAFSLDLSRTKRHLVPGAHFLLQALVQEMSGSEASNVPVKVSATLVSGSDSQVLDIQQSTNGIGQVSISFPIPPTVTELRLLVSAGSLYPAIARLTVQAPPSRGTGFLSIEPLDPRSPSVGDTFILNLQPVGIPAPTFSHYYYMIISRGQIMAMGREPRKTVTSVSVLVDHQLAPSFYFVAYFYHQGHPVANSLLINIQSRDCEGKLQLKVDGAKEYRNADMMKLRIQTDSKALVALGAVDMALYAVGGRSHKPLDMSKVFEVINSYNVGCGPGGGDDALQVFQDAGLAFSDGDRLTQTREDLSCPKEKKSRQKRNVNFQKAVSEKLGQYSSPDAKRCCQDGMTKLPMKRTCEQRAARVPQQACREPFLSCCKFAEDLRRNQTRSQAHLARNNHNMLQEEDLIDEDDILVRTSFPENWLWRVEPVDSSKLLTVWLPDSMTTWEIHGVSLSKSKGLCVAKPTRVRVFRKFHLHLRLPISIRRFEQFELRPVLYNYLNDDVAVSVHVTPVEGLCLAGGGMMAQQVTVPAGSARPVAFSVVPTAAANVPLKVVARGVFDLGDAVSKILQIEKEGAIHREELVYNLDPLNNLGRTLEIPGSSDPNIVPDGDFSSLVRVTASEPLETMGSEGALSPGGVASLLRLPQGCAEQTMIYLAPTLTASNYLDRTEQWSKLSPETKDHAVDLIQKGYMRIQQFRKNDGSFGAWLHRDSSTWLTAFVLKILSLAQEQVGNSPEKLQETASWLLAQQLGDGSFHDPCPVIHRAMQGGLVGSDETVALTAFVVIALHHGLDVFQDDDAKQLKNRVEASITKANSFLGQKASAGLLGAHAAAITAYALTLTKASEDLRNVAHNSLMAMAEETGEHLYWGLVLGSQDKVVLRPTAPRSPTEPVPQAPALWIETTAYALLHLLLREGKGKMADKAASWLTHQGSFHGAFRSTQDTVVTLDALSAYWIASHTTEEKALNVTLSSMGRNGLKTHGLHLNNHQVKGLEEELKILRTYNVLDMKNTTCQDLQIEVKVTGAVEYAWDANEDYEDYYDMPAADDPSVPLQPVTPLQLFEGRRSRRRREAPKVVEEQESRVQYTVCIWRNGKLGLSGMAIADITLLSGFHALRADLEKLTSLSDRYVSHFETDGPHVLLYFDSVPTTRECVGFGASQEVVVGLVQPSSAVLYDYYSPDHKCSVFYAAPTKSQLLATLCSGDVCQCAEGKCPRLLRSLERRVEDKDGYRMRFACYYPRVEYGFTVKVLREDGRAAFRLFESKITQVLHFRKDTMASIGQTRNFLSRASCRLRLEPNKEYLIMGMDGETSDNKGDPQYLLDSNTWIEEMPSEQMCKSTRHRAACFQLKDFLMEFSSRGCQVRQSATHPGFSGLSLGPESSA

Target ORF information:

RefSeq Version	XM_006523532.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component 4B (Chido blood group) (C4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523532.3

ORF Insert Sequence:

ATGCGGCTCC TCTGGGGGCT GGCCTGGGTG TTCAGCTTCT GTGCCTCATC CCTGCAGAAG 
CCCAGGTTGC TCCTGTTTTC CCCTTCTGTG GTTAATTTGG GGACCCCCCT GTCGGTGGGG 
GTACAGCTCC TGGATGCCCC TCCAGGACAG GAGGTAAAAG GATCAGTGTT CCTCAGAAAC 
CCAAAGGGTG GTTCCTGCTC CCCAAAGAAG GACTTTAAGC TGAGCTCGGG AGATGACTTT 
GTGCTGCTCA GCCTTGAGGT CCCACTGGAA GATGTGAGGA GCTGTGGCCT CTTTGACCTG 
CGCAGAGCCC CCCACATCCA GCTGGTAGCT CAGTCTCCGT GGCTAAGGAA CACAGCTTTC 
AAAGCCACAG AGACTCAGGG TGTCAACTTG CTCTTCTCTT CCCGACGAGG CCACATCTTT 
GTGCAGACCG ATCAGCCTAT CTATAATCCG GGGCAGCGGG TTCGTTATCG GGTCTTTGCA 
CTGGATCAAA AGATGCGCCC ATCCACTGAT TTCCTCACCA TCACAGTGGA GAACTCCCAT 
GGCCTCCGTG TACTCAAGAA GGAGATATTT ACTTCCACAT CCATCTTCCA AGATGCCTTC 
ACCATTCCAG ACATCTCAGA GCCTGGGACC TGGAAGATCT CAGCTAGGTT CTCAGATGGA 
CTGGAGTCCA ATAGGAGCAC CCACTTTGAA GTGAAGAAGT ATGTCCTTCC CAACTTCGAG 
GTGAAGATTA CTCCTTGGAA GCCATATATC CTGATGGTGC CCAGCAACAG TGATGAAATC 
CAATTAGACA TCCAGGCCAG GTACATCTAT GGGAAGCCCG TGCAGGGCGT GGCATACACA 
CGGTTTGCGC TCATGGATGA GCAAGGGAAG AGGACTTTCC TTCGGGGCCT AGAGACGCAG 
GCCAAGTTGG TGGAAGGCCG GACCCACATT TCCATCTCAA AGGACCAGTT CCAGGCTGCC 
CTGGATAAAA TCAATATTGG GGTCAGAGAC CTGGAGGGGC TGCGTCTCTA TGCTGCTACA 
GCTGTCATCG AGTCTCCAGG AGGAGAGATG GAGGAGGCAG AACTCACGTC CTGGCGCTTT 
GTATCATCTG CCTTCTCCTT GGATCTCAGC CGCACTAAGA GGCATCTTGT GCCTGGGGCC 
CACTTCCTGC TGCAGGCTCT GGTCCAAGAA ATGTCAGGCT CTGAAGCCTC CAACGTTCCT 
GTTAAAGTCT CTGCCACATT GGTGTCAGGC TCTGACTCCC AAGTCCTTGA CATTCAACAG 
AGCACCAATG GAATTGGCCA AGTCAGCATT TCCTTCCCCA TCCCACCAAC TGTCACAGAA 
CTTCGACTCT TGGTGTCTGC GGGCTCCCTC TACCCAGCCA TAGCCAGGCT CACCGTGCAA 
GCCCCACCTT CAAGAGGCAC TGGCTTTCTT TCTATTGAGC CACTAGACCC TCGGTCCCCT 
AGTGTGGGGG ACACCTTTAT CCTAAACCTT CAACCTGTGG GCATCCCTGC ACCTACCTTC 
TCTCATTACT ACTACATGAT CATCTCCAGA GGCCAGATCA TGGCTATGGG TCGGGAGCCC 
CGGAAGACTG TGACCTCCGT CTCTGTGTTG GTGGACCATC AGCTGGCTCC CTCGTTCTAC 
TTTGTGGCTT ACTTCTATCA CCAAGGACAC CCGGTGGCCA ACTCTCTGCT CATCAACATC 
CAATCCAGGG ACTGTGAGGG CAAGCTGCAA TTGAAGGTGG ATGGTGCCAA GGAGTATCGT 
AATGCGGACA TGATGAAGCT CCGAATTCAA ACTGACTCCA AAGCCCTGGT GGCACTGGGA 
GCTGTGGACA TGGCTCTGTA TGCTGTGGGT GGTCGGTCTC ACAAACCCCT CGACATGAGC 
AAGGTCTTTG AAGTAATCAA CAGCTACAAT GTTGGCTGTG GTCCTGGAGG TGGGGATGAT 
GCCCTTCAGG TGTTCCAGGA TGCTGGTCTG GCCTTTTCTG ATGGTGATCG ACTAACTCAA 
ACCAGAGAGG ACCTGAGCTG TCCCAAGGAG AAGAAAAGTC GGCAAAAGAG AAATGTTAAC 
TTCCAGAAGG CTGTCAGTGA GAAGTTGGGC CAGTATTCTT CTCCAGATGC CAAGCGCTGC 
TGCCAAGACG GGATGACGAA GCTGCCCATG AAGCGTACCT GTGAGCAGCG GGCTGCCCGT 
GTGCCTCAGC AGGCCTGCCG TGAGCCCTTC TTGTCCTGTT GCAAGTTTGC TGAGGACCTT 
CGCAGGAACC AGACCAGGAG CCAGGCACAC CTTGCCCGAA ACAACCACAA CATGCTGCAG 
GAGGAAGACT TGATAGATGA AGACGACATT CTTGTGCGCA CCTCCTTCCC AGAGAACTGG 
CTCTGGAGAG TGGAACCTGT AGACAGCTCC AAACTGTTGA CAGTGTGGCT TCCTGATTCT 
ATGACCACAT GGGAGATTCA TGGTGTGAGC CTGTCCAAAA GCAAAGGTCT GTGTGTAGCC 
AAGCCAACTC GTGTTCGAGT GTTCAGAAAA TTCCACCTGC ACCTGCGCCT GCCCATCTCC 
ATCCGCCGCT TTGAGCAGTT TGAATTACGG CCTGTTCTTT ACAACTATCT GAATGATGAT 
GTGGCTGTGA GTGTCCATGT GACCCCAGTT GAGGGGCTGT GCCTGGCTGG TGGTGGAATG 
ATGGCCCAGC AGGTGACAGT GCCAGCAGGT TCTGCCCGGC CTGTGGCCTT CTCTGTGGTA 
CCCACAGCTG CTGCCAACGT GCCCCTGAAG GTGGTGGCTC GAGGGGTTTT TGATTTAGGG 
GATGCTGTGT CTAAGATTCT CCAAATTGAG AAGGAAGGAG CCATCCACAG AGAAGAGTTA 
GTCTACAACC TCGACCCCCT AAATAACCTG GGTCGGACTT TGGAGATTCC TGGCAGCTCG 
GATCCCAACA TCGTCCCTGA CGGAGACTTC AGCAGCTTAG TCAGGGTTAC AGCCTCGGAA 
CCCTTGGAGA CTATGGGTTC TGAAGGTGCC TTGTCCCCAG GAGGTGTGGC CTCCCTCCTG 
AGGCTTCCCC AGGGCTGTGC AGAGCAAACC ATGATCTATT TGGCTCCTAC CCTGACTGCT 
TCCAACTACC TGGACAGGAC AGAACAGTGG AGCAAACTGT CCCCTGAAAC AAAGGACCAT 
GCTGTGGATC TGATCCAAAA AGGATACATG AGGATCCAGC AGTTTCGGAA GAATGATGGC 
TCCTTTGGGG CTTGGTTACA CCGGGACAGC AGCACCTGGC TGACTGCCTT CGTGCTGAAG 
ATTCTGAGTT TGGCCCAGGA ACAGGTGGGC AACTCCCCGG AGAAGCTGCA GGAGACGGCT 
AGCTGGCTGC TGGCCCAGCA GCTGGGTGAT GGCTCCTTCC ACGACCCATG TCCAGTCATC 
CACAGAGCAA TGCAGGGGGG CTTGGTGGGG TCTGACGAGA CAGTGGCACT GACCGCCTTT 
GTGGTCATTG CCCTTCACCA TGGGTTGGAC GTCTTCCAGG ATGACGATGC GAAGCAGCTG 
AAGAACAGAG TGGAAGCCTC CATCACCAAG GCAAACTCAT TCTTGGGGCA GAAGGCAAGT 
GCTGGGCTCC TGGGTGCCCA TGCCGCCGCC ATCACAGCCT ATGCCCTTAC GCTGACCAAG 
GCCTCGGAGG ACCTGCGGAA TGTTGCCCAC AACAGCCTGA TGGCCATGGC TGAGGAAACA 
GGGGAACACC TCTACTGGGG CTTAGTCCTT GGCTCTCAGG ACAAAGTTGT GTTGCGCCCC 
ACAGCCCCCC GTAGCCCTAC AGAACCTGTG CCCCAGGCCC CAGCCTTGTG GATCGAAACC 
ACAGCCTATG CCCTGCTCCA CCTGCTTCTG CGGGAGGGAA AGGGAAAAAT GGCTGACAAG 
GCTGCATCCT GGCTCACCCA CCAGGGAAGC TTCCATGGGG CATTCCGCAG TACCCAGGAC 
ACTGTGGTCA CCCTGGATGC CCTGTCTGCC TACTGGATCG CTTCGCACAC CACTGAGGAG 
AAAGCACTGA ACGTGACGCT CAGCTCCATG GGCCGCAATG GGCTTAAGAC CCACGGGCTA 
CACTTGAACA ACCACCAAGT CAAGGGCCTG GAGGAGGAGC TAAAGATCCT TCGTACCTAC 
AATGTCCTGG ACATGAAGAA CACCACATGC CAGGACCTTC AGATAGAAGT GAAGGTCACA 
GGCGCTGTGG AATACGCATG GGATGCCAAT GAAGACTACG AAGACTACTA TGACATGCCA 
GCCGCAGATG ACCCCAGCGT CCCCTTGCAG CCTGTCACGC CCCTGCAGCT TTTTGAGGGT 
CGTAGGAGCC GCCGCAGGAG GGAGGCCCCC AAGGTGGTCG AAGAGCAGGA GTCCAGAGTT 
CAGTACACTG TGTGTATCTG GCGAAATGGC AAGCTGGGGC TGTCTGGCAT GGCCATCGCA 
GACATCACCC TCCTAAGTGG ATTCCACGCC CTGAGGGCTG ACCTGGAGAA GCTGACCTCT 
CTCTCTGACC GTTACGTGAG TCACTTTGAG ACTGACGGGC CCCATGTCCT GTTGTACTTT 
GACTCGGTCC CTACCACCCG GGAGTGTGTG GGCTTCGGAG CCTCGCAGGA GGTGGTTGTG 
GGACTGGTGC AGCCATCCAG TGCTGTCCTG TATGACTACT ACAGCCCTGA TCACAAGTGC 
TCTGTGTTTT ATGCTGCACC CACCAAGAGC CAGCTCCTGG CCACACTGTG CTCTGGAGAT 
GTATGCCAGT GCGCTGAGGG GAAGTGCCCT CGACTGCTAA GGTCACTGGA GCGAAGGGTG 
GAGGACAAGG ACGGCTACCG GATGAGGTTC GCCTGCTATT ATCCCCGAGT GGAGTATGGC 
TTCACGGTTA AGGTTCTTCG AGAAGATGGC AGAGCTGCCT TCCGTCTCTT TGAGTCCAAG 
ATCACCCAAG TCCTGCATTT CAGAAAGGAC ACCATGGCCT CCATAGGTCA GACCCGCAAC 
TTCCTGAGCC GGGCCTCTTG CCGCCTTCGT TTGGAGCCTA ACAAAGAGTA CTTGATCATG 
GGGATGGACG GGGAAACCAG TGACAACAAG GGAGACCCCC AGTACTTGCT GGACTCAAAT 
ACCTGGATTG AGGAGATGCC TTCAGAACAA ATGTGCAAGA GCACCCGCCA TCGGGCAGCC 
TGTTTCCAGC TCAAAGACTT CCTGATGGAG TTCAGCAGCC GGGGGTGCCA GGTGCGGCAG 
TCTGCCACCC ACCCAGGATT TTCAGGCCTG AGCCTTGGAC CAGAAAGCTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival.
Cellular microbiology19(12)(2017 Dec)
Caine JA,Lin YP,Kessler JR,Sato H,Leong JM,Coburn J

Synergy between the classical and alternative pathways of complement is essential for conferring effective protection against the pandemic influenza A(H1N1) 2009 virus infection.
PLoS pathogens13(3)e1006248(2017 Mar)
Rattan A,Pawar SD,Nawadkar R,Kulkarni N,Lal G,Mullick J,Sahu A

Collectin-11 detects stress-induced L-fucose pattern to trigger renal epithelial injury.
The Journal of clinical investigation126(5)1911-25(2016 May)
Farrar CA,Tran D,Li K,Wu W,Peng Q,Schwaeble W,Zhou W,Sacks SH

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C4b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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