TBX5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TBX5
Entrez Gene ID	6910
Full Name	T-box 5
Synonyms	HOS
General protein information	Preferred Names T-box 5 Names T-box transcription factor TBX5 T-box protein 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.21
Summary	This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	601620
Disorder Html:	Holt-Oram syndrome, 142900 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_181486.3	NP_852259.1	T-box transcription factor TBX5 isoform 1
NM_000192.3	NP_000183.2	T-box transcription factor TBX5 isoform 1
NM_080717.2	NP_542448.1	T-box transcription factor TBX5 isoform 3
NM_181486.2	NP_852259.1	T-box transcription factor TBX5 isoform 1
XM_017019912.1	XP_016875401.1	T-box transcription factor TBX5 isoform X1
NM_181486.4	NP_852259.1	T-box transcription factor TBX5 isoform 1
NM_080717.3	NP_542448.1	T-box transcription factor TBX5 isoform 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TBX5	XP_001154140.2
Homo sapiens (human)	TBX5	NP_000183.2
Canis lupus familiaris (dog)	TBX5	XP_005636327.1
Gallus gallus (chicken)	TBX5	NP_989504.1
Mus musculus (house mouse)	Tbx5	NP_035667.1
	TBX5	XP_001111737.1
Bos taurus (cattle)	TBX5	NP_001179678.1
Danio rerio (zebrafish)	tbx5a	NP_570990.1
Xenopus tropicalis (tropical clawed frog)	tbx5	NP_001185697.1
Rattus norvegicus (Norway rat)	Tbx5	NP_001009964.1

Gene Ontology
Bibliography

Related articles in PubMed

Downregulation of NFAT3 Due to Lack of T-Box Transcription Factor TBX5 Is Crucial for Cytokine Expression in T Cells.
Kaminuma O, Kitamura N, Nishito Y, Nemoto S, Tatsumi H, Mori A, Hiroi T
Journal of immunology (Baltimore, Md. : 1950)200(1)92-100(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TBX5 deficiency-mediated downregulation of NFAT3 is crucial for the high cytokine-producing activity of T cells
Title: Downregulation of NFAT3 Due to Lack of T-Box Transcription Factor TBX5 Is Crucial for Cytokine Expression in T Cells.

A novel heterozygous missense mutation in TBX5 gene identified in a case of ventricular septal defect. The mutation causes significant changes of the activity of TBX5 in vitro.
Title: Identification of a novel and functional mutation in the TBX5 gene in a patient by screening from 354 patients with isolated ventricular septal defect.

There is no difference in NKX2.5 and TBX5 gene mutations between in vitro fertilization and naturally conceived children with congenital heart disease (CHD).
Title: [NKX2.5 and TBX5 gene mutations in in vitro fertilization children with congenital heart disease].

Findings suggest that a single introduction of the three cardiomyogenic transcription factor (GATA4, cand TBX5)genes using polyethyleneimine (PEI)-based transfection is sufficient for transdifferentiation of adipose-derived stem cells (hADSCs) towards the cardiomyogenic lineage.
Title: A long-lasting cardiomyogenic gene expression by PEI-based transfection induces endogenous cardiac mRNAs in human adipose-derived stem cells.

Rs7312625 of TBx5 gene was significantly associated with lone atrial fibrillation, and snp-snp interaction increased the risk of atrial fibrillation.
Title: Common Variants in the TBX5 Gene Associated with Atrial Fibrillation in a Chinese Han Population.

The following TBX5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBX5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20722	NM_000192.3 Latest version!	Homo sapiens T-box transcription factor 5 (TBX5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu20722	NM_181486.4 Latest version!	Homo sapiens T-box transcription factor 5 (TBX5), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu21706	NM_080717.3 Latest version!	Homo sapiens T-box transcription factor 5 (TBX5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$194.50-$272.30 $389.00
OHu84128	XM_017019912.1 Latest version!	Homo sapiens T-box transcription factor 5 (TBX5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu20722	NM_181486.2	Homo sapiens T-box 5 (TBX5), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu20722	NM_181486.3	Homo sapiens T-box transcription factor 5 (TBX5), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu21706	NM_080717.2	Homo sapiens T-box 5 (TBX5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$194.50-$272.30 $389.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TBX5 ( NM_181486.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_852259.1 Homo sapiens T-box transcription factor 5 (TBX5), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu20722
Clone ID Related Accession (Same CDS sequence)	NM_181486.3 , NM_000192.3 , NM_181486.2 , NM_181486.4
Accession Version	NM_181486.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	T-box transcription factor TBX5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB960757.1, BC027942.1 and AC009260.13. On Jun 2, 2019 this sequence version replaced NM_181486.2. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027942.1, DRR138524.266030.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGACG CAGACGAGGG CTTTGGCCTG GCGCACACGC CTCTGGAGCC TGACGCAAAA 
GACCTGCCCT GCGATTCGAA ACCCGAGAGC GCGCTCGGGG CCCCCAGCAA GTCCCCGTCG 
TCCCCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAGGGAA TCAAAGTGTT TCTCCATGAA 
AGAGAACTGT GGCTAAAATT CCACGAAGTG GGCACGGAAA TGATCATAAC CAAGGCTGGA 
AGGCGGATGT TTCCCAGTTA CAAAGTGAAG GTGACGGGCC TTAATCCCAA AACGAAGTAC 
ATTCTTCTCA TGGACATTGT ACCTGCCGAC GATCACAGAT ACAAATTCGC AGATAATAAA 
TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC ATGCCTGGCC GCCTGTACGT GCACCCAGAC 
TCCCCCGCCA CCGGGGCGCA TTGGATGAGG CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTTGGGCAT ATTATTCTAA ATTCCATGCA CAAATACCAG 
CCTAGATTAC ACATCGTGAA AGCGGATGAA AATAATGGAT TTGGCTCAAA AAATACAGCG 
TTCTGCACTC ACGTCTTTCC TGAGACTGCG TTTATAGCAG TGACTTCCTA CCAGAACCAC 
AAGATCACGC AATTAAAGAT TGAGAATAAT CCCTTTGCCA AAGGATTTCG GGGCAGTGAT 
GACATGGAGC TGCACAGAAT GTCAAGAATG CAAAGTAAAG AATATCCCGT GGTCCCCAGG 
AGCACCGTGA GGCAAAAAGT GGCCTCCAAC CACAGTCCTT TCAGCAGCGA GTCTCGAGCT 
CTCTCCACCT CATCCAATTT GGGGTCCCAA TACCAGTGTG AGAATGGTGT TTCCGGCCCC 
TCCCAGGACC TCCTGCCTCC ACCCAACCCA TACCCACTGC CCCAGGAGCA TAGCCAAATT 
TACCATTGTA CCAAGAGGAA AGAGGAAGAA TGTTCCACCA CAGACCATCC CTATAAGAAG 
CCCTACATGG AGACATCACC CAGTGAAGAA GATTCCTTCT ACCGCTCTAG CTATCCACAG 
CAGCAGGGCC TGGGTGCCTC CTACAGGACA GAGTCGGCAC AGCGGCAAGC TTGCATGTAT 
GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG CCCAGCCTAG AGGACATCAG CTGCAACACG 
TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC ACCGTCACCA CCGTGCAGCC CATGGACAGG 
CTACCCTACC AGCACTTCTC CGCTCACTTC ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT 
GGCATGGCCA ACCATGGCTC CCCACAGCTG GGAGAGGGAA TGTTCCAGCA CCAGACCTCC 
GTGGCCCACC AGCCTGTGGT CAGGCAGTGT GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC 
ACCCTTCAGC CCCCTGAGTT CCTCTACTCT CATGGCGTGC CAAGGACTCT ATCCCCTCAT 
CAGTACCACT CTGTGCACGG AGTTGGCATG GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852259.1
CDS	556..2112
Translation	MADADEGFGLAHTPLEPDAKDLPCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRALSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKEEECSTTDHPYKKPYMETSPSEEDSFYRSSYPQQQGLGASYRTESAQRQACMYASSAPPSEPVPSLEDISCNTWPSMPSYSSCTVTTVQPMDRLPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPGTLQPPEFLYSHGVPRTLSPHQYHSVHGVGMVPEWSDNS

Target ORF information:

RefSeq Version	NM_181486.3
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box transcription factor 5 (TBX5), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181486.3

ORF Insert Sequence:

ATGGCCGACG CAGACGAGGG CTTTGGCCTG GCGCACACGC CTCTGGAGCC TGACGCAAAA 
GACCTGCCCT GCGATTCGAA ACCCGAGAGC GCGCTCGGGG CCCCCAGCAA GTCCCCGTCG 
TCCCCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAGGGAA TCAAAGTGTT TCTCCATGAA 
AGAGAACTGT GGCTAAAATT CCACGAAGTG GGCACGGAAA TGATCATAAC CAAGGCTGGA 
AGGCGGATGT TTCCCAGTTA CAAAGTGAAG GTGACGGGCC TTAATCCCAA AACGAAGTAC 
ATTCTTCTCA TGGACATTGT ACCTGCCGAC GATCACAGAT ACAAATTCGC AGATAATAAA 
TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC ATGCCTGGCC GCCTGTACGT GCACCCAGAC 
TCCCCCGCCA CCGGGGCGCA TTGGATGAGG CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTTGGGCAT ATTATTCTAA ATTCCATGCA CAAATACCAG 
CCTAGATTAC ACATCGTGAA AGCGGATGAA AATAATGGAT TTGGCTCAAA AAATACAGCG 
TTCTGCACTC ACGTCTTTCC TGAGACTGCG TTTATAGCAG TGACTTCCTA CCAGAACCAC 
AAGATCACGC AATTAAAGAT TGAGAATAAT CCCTTTGCCA AAGGATTTCG GGGCAGTGAT 
GACATGGAGC TGCACAGAAT GTCAAGAATG CAAAGTAAAG AATATCCCGT GGTCCCCAGG 
AGCACCGTGA GGCAAAAAGT GGCCTCCAAC CACAGTCCTT TCAGCAGCGA GTCTCGAGCT 
CTCTCCACCT CATCCAATTT GGGGTCCCAA TACCAGTGTG AGAATGGTGT TTCCGGCCCC 
TCCCAGGACC TCCTGCCTCC ACCCAACCCA TACCCACTGC CCCAGGAGCA TAGCCAAATT 
TACCATTGTA CCAAGAGGAA AGAGGAAGAA TGTTCCACCA CAGACCATCC CTATAAGAAG 
CCCTACATGG AGACATCACC CAGTGAAGAA GATTCCTTCT ACCGCTCTAG CTATCCACAG 
CAGCAGGGCC TGGGTGCCTC CTACAGGACA GAGTCGGCAC AGCGGCAAGC TTGCATGTAT 
GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG CCCAGCCTAG AGGACATCAG CTGCAACACG 
TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC ACCGTCACCA CCGTGCAGCC CATGGACAGG 
CTACCCTACC AGCACTTCTC CGCTCACTTC ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT 
GGCATGGCCA ACCATGGCTC CCCACAGCTG GGAGAGGGAA TGTTCCAGCA CCAGACCTCC 
GTGGCCCACC AGCCTGTGGT CAGGCAGTGT GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC 
ACCCTTCAGC CCCCTGAGTT CCTCTACTCT CATGGCGTGC CAAGGACTCT ATCCCCTCAT 
CAGTACCACT CTGTGCACGG AGTTGGCATG GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBX5 ( NM_000192.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000183.2 Homo sapiens T-box transcription factor 5 (TBX5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20722
Clone ID Related Accession (Same CDS sequence)	NM_181486.3 , NM_000192.3 , NM_181486.2 , NM_181486.4
Accession Version	NM_000192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-02
Organism	Homo sapiens(human)
Product	T-box transcription factor TBX5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF221714.1, DA863182.1, AC009260.13 and BC027942.1. This sequence is a reference standard in the RefSeqGene project. On Jun 12, 2003 this sequence version replaced NM_000192.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF221714.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGACG CAGACGAGGG CTTTGGCCTG GCGCACACGC CTCTGGAGCC TGACGCAAAA 
GACCTGCCCT GCGATTCGAA ACCCGAGAGC GCGCTCGGGG CCCCCAGCAA GTCCCCGTCG 
TCCCCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAGGGAA TCAAAGTGTT TCTCCATGAA 
AGAGAACTGT GGCTAAAATT CCACGAAGTG GGCACGGAAA TGATCATAAC CAAGGCTGGA 
AGGCGGATGT TTCCCAGTTA CAAAGTGAAG GTGACGGGCC TTAATCCCAA AACGAAGTAC 
ATTCTTCTCA TGGACATTGT ACCTGCCGAC GATCACAGAT ACAAATTCGC AGATAATAAA 
TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC ATGCCTGGCC GCCTGTACGT GCACCCAGAC 
TCCCCCGCCA CCGGGGCGCA TTGGATGAGG CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTTGGGCAT ATTATTCTAA ATTCCATGCA CAAATACCAG 
CCTAGATTAC ACATCGTGAA AGCGGATGAA AATAATGGAT TTGGCTCAAA AAATACAGCG 
TTCTGCACTC ACGTCTTTCC TGAGACTGCG TTTATAGCAG TGACTTCCTA CCAGAACCAC 
AAGATCACGC AATTAAAGAT TGAGAATAAT CCCTTTGCCA AAGGATTTCG GGGCAGTGAT 
GACATGGAGC TGCACAGAAT GTCAAGAATG CAAAGTAAAG AATATCCCGT GGTCCCCAGG 
AGCACCGTGA GGCAAAAAGT GGCCTCCAAC CACAGTCCTT TCAGCAGCGA GTCTCGAGCT 
CTCTCCACCT CATCCAATTT GGGGTCCCAA TACCAGTGTG AGAATGGTGT TTCCGGCCCC 
TCCCAGGACC TCCTGCCTCC ACCCAACCCA TACCCACTGC CCCAGGAGCA TAGCCAAATT 
TACCATTGTA CCAAGAGGAA AGAGGAAGAA TGTTCCACCA CAGACCATCC CTATAAGAAG 
CCCTACATGG AGACATCACC CAGTGAAGAA GATTCCTTCT ACCGCTCTAG CTATCCACAG 
CAGCAGGGCC TGGGTGCCTC CTACAGGACA GAGTCGGCAC AGCGGCAAGC TTGCATGTAT 
GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG CCCAGCCTAG AGGACATCAG CTGCAACACG 
TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC ACCGTCACCA CCGTGCAGCC CATGGACAGG 
CTACCCTACC AGCACTTCTC CGCTCACTTC ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT 
GGCATGGCCA ACCATGGCTC CCCACAGCTG GGAGAGGGAA TGTTCCAGCA CCAGACCTCC 
GTGGCCCACC AGCCTGTGGT CAGGCAGTGT GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC 
ACCCTTCAGC CCCCTGAGTT CCTCTACTCT CATGGCGTGC CAAGGACTCT ATCCCCTCAT 
CAGTACCACT CTGTGCACGG AGTTGGCATG GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000183.2
CDS	668..2224
Translation	MADADEGFGLAHTPLEPDAKDLPCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRALSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKEEECSTTDHPYKKPYMETSPSEEDSFYRSSYPQQQGLGASYRTESAQRQACMYASSAPPSEPVPSLEDISCNTWPSMPSYSSCTVTTVQPMDRLPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPGTLQPPEFLYSHGVPRTLSPHQYHSVHGVGMVPEWSDNS

Target ORF information:

RefSeq Version	NM_000192.3
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box transcription factor 5 (TBX5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000192.3

ORF Insert Sequence:

ATGGCCGACG CAGACGAGGG CTTTGGCCTG GCGCACACGC CTCTGGAGCC TGACGCAAAA 
GACCTGCCCT GCGATTCGAA ACCCGAGAGC GCGCTCGGGG CCCCCAGCAA GTCCCCGTCG 
TCCCCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAGGGAA TCAAAGTGTT TCTCCATGAA 
AGAGAACTGT GGCTAAAATT CCACGAAGTG GGCACGGAAA TGATCATAAC CAAGGCTGGA 
AGGCGGATGT TTCCCAGTTA CAAAGTGAAG GTGACGGGCC TTAATCCCAA AACGAAGTAC 
ATTCTTCTCA TGGACATTGT ACCTGCCGAC GATCACAGAT ACAAATTCGC AGATAATAAA 
TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC ATGCCTGGCC GCCTGTACGT GCACCCAGAC 
TCCCCCGCCA CCGGGGCGCA TTGGATGAGG CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTTGGGCAT ATTATTCTAA ATTCCATGCA CAAATACCAG 
CCTAGATTAC ACATCGTGAA AGCGGATGAA AATAATGGAT TTGGCTCAAA AAATACAGCG 
TTCTGCACTC ACGTCTTTCC TGAGACTGCG TTTATAGCAG TGACTTCCTA CCAGAACCAC 
AAGATCACGC AATTAAAGAT TGAGAATAAT CCCTTTGCCA AAGGATTTCG GGGCAGTGAT 
GACATGGAGC TGCACAGAAT GTCAAGAATG CAAAGTAAAG AATATCCCGT GGTCCCCAGG 
AGCACCGTGA GGCAAAAAGT GGCCTCCAAC CACAGTCCTT TCAGCAGCGA GTCTCGAGCT 
CTCTCCACCT CATCCAATTT GGGGTCCCAA TACCAGTGTG AGAATGGTGT TTCCGGCCCC 
TCCCAGGACC TCCTGCCTCC ACCCAACCCA TACCCACTGC CCCAGGAGCA TAGCCAAATT 
TACCATTGTA CCAAGAGGAA AGAGGAAGAA TGTTCCACCA CAGACCATCC CTATAAGAAG 
CCCTACATGG AGACATCACC CAGTGAAGAA GATTCCTTCT ACCGCTCTAG CTATCCACAG 
CAGCAGGGCC TGGGTGCCTC CTACAGGACA GAGTCGGCAC AGCGGCAAGC TTGCATGTAT 
GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG CCCAGCCTAG AGGACATCAG CTGCAACACG 
TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC ACCGTCACCA CCGTGCAGCC CATGGACAGG 
CTACCCTACC AGCACTTCTC CGCTCACTTC ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT 
GGCATGGCCA ACCATGGCTC CCCACAGCTG GGAGAGGGAA TGTTCCAGCA CCAGACCTCC 
GTGGCCCACC AGCCTGTGGT CAGGCAGTGT GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC 
ACCCTTCAGC CCCCTGAGTT CCTCTACTCT CATGGCGTGC CAAGGACTCT ATCCCCTCAT 
CAGTACCACT CTGTGCACGG AGTTGGCATG GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBX5 ( NM_181486.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_852259.1 Homo sapiens T-box 5 (TBX5), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu20722
Clone ID Related Accession (Same CDS sequence)	NM_181486.3 , NM_000192.3 , NM_181486.2 , NM_181486.4
Accession Version	NM_181486.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	T-box transcription factor TBX5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB960757.1, BC027942.1 and AC009260.13. On May 12, 2012 this sequence version replaced NM_181486.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027942.1, AK290611.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGACG CAGACGAGGG CTTTGGCCTG GCGCACACGC CTCTGGAGCC TGACGCAAAA 
GACCTGCCCT GCGATTCGAA ACCCGAGAGC GCGCTCGGGG CCCCCAGCAA GTCCCCGTCG 
TCCCCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAGGGAA TCAAAGTGTT TCTCCATGAA 
AGAGAACTGT GGCTAAAATT CCACGAAGTG GGCACGGAAA TGATCATAAC CAAGGCTGGA 
AGGCGGATGT TTCCCAGTTA CAAAGTGAAG GTGACGGGCC TTAATCCCAA AACGAAGTAC 
ATTCTTCTCA TGGACATTGT ACCTGCCGAC GATCACAGAT ACAAATTCGC AGATAATAAA 
TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC ATGCCTGGCC GCCTGTACGT GCACCCAGAC 
TCCCCCGCCA CCGGGGCGCA TTGGATGAGG CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTTGGGCAT ATTATTCTAA ATTCCATGCA CAAATACCAG 
CCTAGATTAC ACATCGTGAA AGCGGATGAA AATAATGGAT TTGGCTCAAA AAATACAGCG 
TTCTGCACTC ACGTCTTTCC TGAGACTGCG TTTATAGCAG TGACTTCCTA CCAGAACCAC 
AAGATCACGC AATTAAAGAT TGAGAATAAT CCCTTTGCCA AAGGATTTCG GGGCAGTGAT 
GACATGGAGC TGCACAGAAT GTCAAGAATG CAAAGTAAAG AATATCCCGT GGTCCCCAGG 
AGCACCGTGA GGCAAAAAGT GGCCTCCAAC CACAGTCCTT TCAGCAGCGA GTCTCGAGCT 
CTCTCCACCT CATCCAATTT GGGGTCCCAA TACCAGTGTG AGAATGGTGT TTCCGGCCCC 
TCCCAGGACC TCCTGCCTCC ACCCAACCCA TACCCACTGC CCCAGGAGCA TAGCCAAATT 
TACCATTGTA CCAAGAGGAA AGAGGAAGAA TGTTCCACCA CAGACCATCC CTATAAGAAG 
CCCTACATGG AGACATCACC CAGTGAAGAA GATTCCTTCT ACCGCTCTAG CTATCCACAG 
CAGCAGGGCC TGGGTGCCTC CTACAGGACA GAGTCGGCAC AGCGGCAAGC TTGCATGTAT 
GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG CCCAGCCTAG AGGACATCAG CTGCAACACG 
TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC ACCGTCACCA CCGTGCAGCC CATGGACAGG 
CTACCCTACC AGCACTTCTC CGCTCACTTC ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT 
GGCATGGCCA ACCATGGCTC CCCACAGCTG GGAGAGGGAA TGTTCCAGCA CCAGACCTCC 
GTGGCCCACC AGCCTGTGGT CAGGCAGTGT GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC 
ACCCTTCAGC CCCCTGAGTT CCTCTACTCT CATGGCGTGC CAAGGACTCT ATCCCCTCAT 
CAGTACCACT CTGTGCACGG AGTTGGCATG GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852259.1
CDS	575..2131
Translation	MADADEGFGLAHTPLEPDAKDLPCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRALSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKEEECSTTDHPYKKPYMETSPSEEDSFYRSSYPQQQGLGASYRTESAQRQACMYASSAPPSEPVPSLEDISCNTWPSMPSYSSCTVTTVQPMDRLPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPGTLQPPEFLYSHGVPRTLSPHQYHSVHGVGMVPEWSDNS

Target ORF information:

RefSeq Version	NM_181486.2
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box 5 (TBX5), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181486.2

ORF Insert Sequence:

ATGGCCGACG CAGACGAGGG CTTTGGCCTG GCGCACACGC CTCTGGAGCC TGACGCAAAA 
GACCTGCCCT GCGATTCGAA ACCCGAGAGC GCGCTCGGGG CCCCCAGCAA GTCCCCGTCG 
TCCCCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAGGGAA TCAAAGTGTT TCTCCATGAA 
AGAGAACTGT GGCTAAAATT CCACGAAGTG GGCACGGAAA TGATCATAAC CAAGGCTGGA 
AGGCGGATGT TTCCCAGTTA CAAAGTGAAG GTGACGGGCC TTAATCCCAA AACGAAGTAC 
ATTCTTCTCA TGGACATTGT ACCTGCCGAC GATCACAGAT ACAAATTCGC AGATAATAAA 
TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC ATGCCTGGCC GCCTGTACGT GCACCCAGAC 
TCCCCCGCCA CCGGGGCGCA TTGGATGAGG CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTTGGGCAT ATTATTCTAA ATTCCATGCA CAAATACCAG 
CCTAGATTAC ACATCGTGAA AGCGGATGAA AATAATGGAT TTGGCTCAAA AAATACAGCG 
TTCTGCACTC ACGTCTTTCC TGAGACTGCG TTTATAGCAG TGACTTCCTA CCAGAACCAC 
AAGATCACGC AATTAAAGAT TGAGAATAAT CCCTTTGCCA AAGGATTTCG GGGCAGTGAT 
GACATGGAGC TGCACAGAAT GTCAAGAATG CAAAGTAAAG AATATCCCGT GGTCCCCAGG 
AGCACCGTGA GGCAAAAAGT GGCCTCCAAC CACAGTCCTT TCAGCAGCGA GTCTCGAGCT 
CTCTCCACCT CATCCAATTT GGGGTCCCAA TACCAGTGTG AGAATGGTGT TTCCGGCCCC 
TCCCAGGACC TCCTGCCTCC ACCCAACCCA TACCCACTGC CCCAGGAGCA TAGCCAAATT 
TACCATTGTA CCAAGAGGAA AGAGGAAGAA TGTTCCACCA CAGACCATCC CTATAAGAAG 
CCCTACATGG AGACATCACC CAGTGAAGAA GATTCCTTCT ACCGCTCTAG CTATCCACAG 
CAGCAGGGCC TGGGTGCCTC CTACAGGACA GAGTCGGCAC AGCGGCAAGC TTGCATGTAT 
GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG CCCAGCCTAG AGGACATCAG CTGCAACACG 
TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC ACCGTCACCA CCGTGCAGCC CATGGACAGG 
CTACCCTACC AGCACTTCTC CGCTCACTTC ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT 
GGCATGGCCA ACCATGGCTC CCCACAGCTG GGAGAGGGAA TGTTCCAGCA CCAGACCTCC 
GTGGCCCACC AGCCTGTGGT CAGGCAGTGT GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC 
ACCCTTCAGC CCCCTGAGTT CCTCTACTCT CATGGCGTGC CAAGGACTCT ATCCCCTCAT 
CAGTACCACT CTGTGCACGG AGTTGGCATG GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBX5 ( NM_181486.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_852259.1 Homo sapiens T-box transcription factor 5 (TBX5), transcript variant 4, mRNA.

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CloneID	OHu20722
Clone ID Related Accession (Same CDS sequence)	NM_181486.3 , NM_000192.3 , NM_181486.2 , NM_181486.4
Accession Version	NM_181486.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-11
Organism	Homo sapiens(human)
Product	T-box transcription factor TBX5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB960757.1, BC027942.1 and AC009260.13. On Dec 2, 2019 this sequence version replaced NM_181486.3. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1 and 4 both encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027942.1, AK290611.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGACG CAGACGAGGG CTTTGGCCTG GCGCACACGC CTCTGGAGCC TGACGCAAAA 
GACCTGCCCT GCGATTCGAA ACCCGAGAGC GCGCTCGGGG CCCCCAGCAA GTCCCCGTCG 
TCCCCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAGGGAA TCAAAGTGTT TCTCCATGAA 
AGAGAACTGT GGCTAAAATT CCACGAAGTG GGCACGGAAA TGATCATAAC CAAGGCTGGA 
AGGCGGATGT TTCCCAGTTA CAAAGTGAAG GTGACGGGCC TTAATCCCAA AACGAAGTAC 
ATTCTTCTCA TGGACATTGT ACCTGCCGAC GATCACAGAT ACAAATTCGC AGATAATAAA 
TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC ATGCCTGGCC GCCTGTACGT GCACCCAGAC 
TCCCCCGCCA CCGGGGCGCA TTGGATGAGG CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTTGGGCAT ATTATTCTAA ATTCCATGCA CAAATACCAG 
CCTAGATTAC ACATCGTGAA AGCGGATGAA AATAATGGAT TTGGCTCAAA AAATACAGCG 
TTCTGCACTC ACGTCTTTCC TGAGACTGCG TTTATAGCAG TGACTTCCTA CCAGAACCAC 
AAGATCACGC AATTAAAGAT TGAGAATAAT CCCTTTGCCA AAGGATTTCG GGGCAGTGAT 
GACATGGAGC TGCACAGAAT GTCAAGAATG CAAAGTAAAG AATATCCCGT GGTCCCCAGG 
AGCACCGTGA GGCAAAAAGT GGCCTCCAAC CACAGTCCTT TCAGCAGCGA GTCTCGAGCT 
CTCTCCACCT CATCCAATTT GGGGTCCCAA TACCAGTGTG AGAATGGTGT TTCCGGCCCC 
TCCCAGGACC TCCTGCCTCC ACCCAACCCA TACCCACTGC CCCAGGAGCA TAGCCAAATT 
TACCATTGTA CCAAGAGGAA AGAGGAAGAA TGTTCCACCA CAGACCATCC CTATAAGAAG 
CCCTACATGG AGACATCACC CAGTGAAGAA GATTCCTTCT ACCGCTCTAG CTATCCACAG 
CAGCAGGGCC TGGGTGCCTC CTACAGGACA GAGTCGGCAC AGCGGCAAGC TTGCATGTAT 
GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG CCCAGCCTAG AGGACATCAG CTGCAACACG 
TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC ACCGTCACCA CCGTGCAGCC CATGGACAGG 
CTACCCTACC AGCACTTCTC CGCTCACTTC ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT 
GGCATGGCCA ACCATGGCTC CCCACAGCTG GGAGAGGGAA TGTTCCAGCA CCAGACCTCC 
GTGGCCCACC AGCCTGTGGT CAGGCAGTGT GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC 
ACCCTTCAGC CCCCTGAGTT CCTCTACTCT CATGGCGTGC CAAGGACTCT ATCCCCTCAT 
CAGTACCACT CTGTGCACGG AGTTGGCATG GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852259.1
CDS	556..2112
Translation	MADADEGFGLAHTPLEPDAKDLPCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRALSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKEEECSTTDHPYKKPYMETSPSEEDSFYRSSYPQQQGLGASYRTESAQRQACMYASSAPPSEPVPSLEDISCNTWPSMPSYSSCTVTTVQPMDRLPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPGTLQPPEFLYSHGVPRTLSPHQYHSVHGVGMVPEWSDNS

Target ORF information:

RefSeq Version	NM_181486.4
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box transcription factor 5 (TBX5), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181486.4

ORF Insert Sequence:

ATGGCCGACG CAGACGAGGG CTTTGGCCTG GCGCACACGC CTCTGGAGCC TGACGCAAAA 
GACCTGCCCT GCGATTCGAA ACCCGAGAGC GCGCTCGGGG CCCCCAGCAA GTCCCCGTCG 
TCCCCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAGGGAA TCAAAGTGTT TCTCCATGAA 
AGAGAACTGT GGCTAAAATT CCACGAAGTG GGCACGGAAA TGATCATAAC CAAGGCTGGA 
AGGCGGATGT TTCCCAGTTA CAAAGTGAAG GTGACGGGCC TTAATCCCAA AACGAAGTAC 
ATTCTTCTCA TGGACATTGT ACCTGCCGAC GATCACAGAT ACAAATTCGC AGATAATAAA 
TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC ATGCCTGGCC GCCTGTACGT GCACCCAGAC 
TCCCCCGCCA CCGGGGCGCA TTGGATGAGG CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTTGGGCAT ATTATTCTAA ATTCCATGCA CAAATACCAG 
CCTAGATTAC ACATCGTGAA AGCGGATGAA AATAATGGAT TTGGCTCAAA AAATACAGCG 
TTCTGCACTC ACGTCTTTCC TGAGACTGCG TTTATAGCAG TGACTTCCTA CCAGAACCAC 
AAGATCACGC AATTAAAGAT TGAGAATAAT CCCTTTGCCA AAGGATTTCG GGGCAGTGAT 
GACATGGAGC TGCACAGAAT GTCAAGAATG CAAAGTAAAG AATATCCCGT GGTCCCCAGG 
AGCACCGTGA GGCAAAAAGT GGCCTCCAAC CACAGTCCTT TCAGCAGCGA GTCTCGAGCT 
CTCTCCACCT CATCCAATTT GGGGTCCCAA TACCAGTGTG AGAATGGTGT TTCCGGCCCC 
TCCCAGGACC TCCTGCCTCC ACCCAACCCA TACCCACTGC CCCAGGAGCA TAGCCAAATT 
TACCATTGTA CCAAGAGGAA AGAGGAAGAA TGTTCCACCA CAGACCATCC CTATAAGAAG 
CCCTACATGG AGACATCACC CAGTGAAGAA GATTCCTTCT ACCGCTCTAG CTATCCACAG 
CAGCAGGGCC TGGGTGCCTC CTACAGGACA GAGTCGGCAC AGCGGCAAGC TTGCATGTAT 
GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG CCCAGCCTAG AGGACATCAG CTGCAACACG 
TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC ACCGTCACCA CCGTGCAGCC CATGGACAGG 
CTACCCTACC AGCACTTCTC CGCTCACTTC ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT 
GGCATGGCCA ACCATGGCTC CCCACAGCTG GGAGAGGGAA TGTTCCAGCA CCAGACCTCC 
GTGGCCCACC AGCCTGTGGT CAGGCAGTGT GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC 
ACCCTTCAGC CCCCTGAGTT CCTCTACTCT CATGGCGTGC CAAGGACTCT ATCCCCTCAT 
CAGTACCACT CTGTGCACGG AGTTGGCATG GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBX5 ( NM_080717.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_542448.1 Homo sapiens T-box 5 (TBX5), transcript variant 3, mRNA.

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CloneID	OHu21706
Clone ID Related Accession (Same CDS sequence)	NM_080717.2 , NM_080717.3
Accession Version	NM_080717.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	T-box transcription factor TBX5 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF221714.1, AB051068.1, AC009260.13 and BC027942.1. On Jun 12, 2003 this sequence version replaced NM_080717.1. Transcript Variant: This variant (3) lacks the exon containing the translation start site compared to transcript variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB051068.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGAA TCAAAGTGTT TCTCCATGAA AGAGAACTGT GGCTAAAATT CCACGAAGTG 
GGCACGGAAA TGATCATAAC CAAGGCTGGA AGGCGGATGT TTCCCAGTTA CAAAGTGAAG 
GTGACGGGCC TTAATCCCAA AACGAAGTAC ATTCTTCTCA TGGACATTGT ACCTGCCGAC 
GATCACAGAT ACAAATTCGC AGATAATAAA TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC 
ATGCCTGGCC GCCTGTACGT GCACCCAGAC TCCCCCGCCA CCGGGGCGCA TTGGATGAGG 
CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC ACCAACAACC ACCTGGACCC ATTTGGGCAT 
ATTATTCTAA ATTCCATGCA CAAATACCAG CCTAGATTAC ACATCGTGAA AGCGGATGAA 
AATAATGGAT TTGGCTCAAA AAATACAGCG TTCTGCACTC ACGTCTTTCC TGAGACTGCG 
TTTATAGCAG TGACTTCCTA CCAGAACCAC AAGATCACGC AATTAAAGAT TGAGAATAAT 
CCCTTTGCCA AAGGATTTCG GGGCAGTGAT GACATGGAGC TGCACAGAAT GTCAAGAATG 
CAAAGTAAAG AATATCCCGT GGTCCCCAGG AGCACCGTGA GGCAAAAAGT GGCCTCCAAC 
CACAGTCCTT TCAGCAGCGA GTCTCGAGCT CTCTCCACCT CATCCAATTT GGGGTCCCAA 
TACCAGTGTG AGAATGGTGT TTCCGGCCCC TCCCAGGACC TCCTGCCTCC ACCCAACCCA 
TACCCACTGC CCCAGGAGCA TAGCCAAATT TACCATTGTA CCAAGAGGAA AGAGGAAGAA 
TGTTCCACCA CAGACCATCC CTATAAGAAG CCCTACATGG AGACATCACC CAGTGAAGAA 
GATTCCTTCT ACCGCTCTAG CTATCCACAG CAGCAGGGCC TGGGTGCCTC CTACAGGACA 
GAGTCGGCAC AGCGGCAAGC TTGCATGTAT GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG 
CCCAGCCTAG AGGACATCAG CTGCAACACG TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC 
ACCGTCACCA CCGTGCAGCC CATGGACAGG CTACCCTACC AGCACTTCTC CGCTCACTTC 
ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT GGCATGGCCA ACCATGGCTC CCCACAGCTG 
GGAGAGGGAA TGTTCCAGCA CCAGACCTCC GTGGCCCACC AGCCTGTGGT CAGGCAGTGT 
GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC ACCCTTCAGC CCCCTGAGTT CCTCTACTCT 
CATGGCGTGC CAAGGACTCT ATCCCCTCAT CAGTACCACT CTGTGCACGG AGTTGGCATG 
GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542448.1
CDS	633..2039
Translation	MEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRALSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKEEECSTTDHPYKKPYMETSPSEEDSFYRSSYPQQQGLGASYRTESAQRQACMYASSAPPSEPVPSLEDISCNTWPSMPSYSSCTVTTVQPMDRLPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPGTLQPPEFLYSHGVPRTLSPHQYHSVHGVGMVPEWSDNS

Target ORF information:

RefSeq Version	NM_080717.2
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box 5 (TBX5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080717.2

ORF Insert Sequence:

ATGGAGGGAA TCAAAGTGTT TCTCCATGAA AGAGAACTGT GGCTAAAATT CCACGAAGTG 
GGCACGGAAA TGATCATAAC CAAGGCTGGA AGGCGGATGT TTCCCAGTTA CAAAGTGAAG 
GTGACGGGCC TTAATCCCAA AACGAAGTAC ATTCTTCTCA TGGACATTGT ACCTGCCGAC 
GATCACAGAT ACAAATTCGC AGATAATAAA TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC 
ATGCCTGGCC GCCTGTACGT GCACCCAGAC TCCCCCGCCA CCGGGGCGCA TTGGATGAGG 
CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC ACCAACAACC ACCTGGACCC ATTTGGGCAT 
ATTATTCTAA ATTCCATGCA CAAATACCAG CCTAGATTAC ACATCGTGAA AGCGGATGAA 
AATAATGGAT TTGGCTCAAA AAATACAGCG TTCTGCACTC ACGTCTTTCC TGAGACTGCG 
TTTATAGCAG TGACTTCCTA CCAGAACCAC AAGATCACGC AATTAAAGAT TGAGAATAAT 
CCCTTTGCCA AAGGATTTCG GGGCAGTGAT GACATGGAGC TGCACAGAAT GTCAAGAATG 
CAAAGTAAAG AATATCCCGT GGTCCCCAGG AGCACCGTGA GGCAAAAAGT GGCCTCCAAC 
CACAGTCCTT TCAGCAGCGA GTCTCGAGCT CTCTCCACCT CATCCAATTT GGGGTCCCAA 
TACCAGTGTG AGAATGGTGT TTCCGGCCCC TCCCAGGACC TCCTGCCTCC ACCCAACCCA 
TACCCACTGC CCCAGGAGCA TAGCCAAATT TACCATTGTA CCAAGAGGAA AGAGGAAGAA 
TGTTCCACCA CAGACCATCC CTATAAGAAG CCCTACATGG AGACATCACC CAGTGAAGAA 
GATTCCTTCT ACCGCTCTAG CTATCCACAG CAGCAGGGCC TGGGTGCCTC CTACAGGACA 
GAGTCGGCAC AGCGGCAAGC TTGCATGTAT GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG 
CCCAGCCTAG AGGACATCAG CTGCAACACG TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC 
ACCGTCACCA CCGTGCAGCC CATGGACAGG CTACCCTACC AGCACTTCTC CGCTCACTTC 
ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT GGCATGGCCA ACCATGGCTC CCCACAGCTG 
GGAGAGGGAA TGTTCCAGCA CCAGACCTCC GTGGCCCACC AGCCTGTGGT CAGGCAGTGT 
GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC ACCCTTCAGC CCCCTGAGTT CCTCTACTCT 
CATGGCGTGC CAAGGACTCT ATCCCCTCAT CAGTACCACT CTGTGCACGG AGTTGGCATG 
GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBX5 ( NM_080717.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_542448.1 Homo sapiens T-box transcription factor 5 (TBX5), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu21706
Clone ID Related Accession (Same CDS sequence)	NM_080717.2 , NM_080717.3
Accession Version	NM_080717.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	T-box transcription factor TBX5 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB051068.1, AF221714.1, AC009260.13 and BC027942.1. On Jun 2, 2019 this sequence version replaced NM_080717.2. Transcript Variant: This variant (3) lacks the exon containing the translation start site compared to transcript variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB051068.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGAA TCAAAGTGTT TCTCCATGAA AGAGAACTGT GGCTAAAATT CCACGAAGTG 
GGCACGGAAA TGATCATAAC CAAGGCTGGA AGGCGGATGT TTCCCAGTTA CAAAGTGAAG 
GTGACGGGCC TTAATCCCAA AACGAAGTAC ATTCTTCTCA TGGACATTGT ACCTGCCGAC 
GATCACAGAT ACAAATTCGC AGATAATAAA TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC 
ATGCCTGGCC GCCTGTACGT GCACCCAGAC TCCCCCGCCA CCGGGGCGCA TTGGATGAGG 
CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC ACCAACAACC ACCTGGACCC ATTTGGGCAT 
ATTATTCTAA ATTCCATGCA CAAATACCAG CCTAGATTAC ACATCGTGAA AGCGGATGAA 
AATAATGGAT TTGGCTCAAA AAATACAGCG TTCTGCACTC ACGTCTTTCC TGAGACTGCG 
TTTATAGCAG TGACTTCCTA CCAGAACCAC AAGATCACGC AATTAAAGAT TGAGAATAAT 
CCCTTTGCCA AAGGATTTCG GGGCAGTGAT GACATGGAGC TGCACAGAAT GTCAAGAATG 
CAAAGTAAAG AATATCCCGT GGTCCCCAGG AGCACCGTGA GGCAAAAAGT GGCCTCCAAC 
CACAGTCCTT TCAGCAGCGA GTCTCGAGCT CTCTCCACCT CATCCAATTT GGGGTCCCAA 
TACCAGTGTG AGAATGGTGT TTCCGGCCCC TCCCAGGACC TCCTGCCTCC ACCCAACCCA 
TACCCACTGC CCCAGGAGCA TAGCCAAATT TACCATTGTA CCAAGAGGAA AGAGGAAGAA 
TGTTCCACCA CAGACCATCC CTATAAGAAG CCCTACATGG AGACATCACC CAGTGAAGAA 
GATTCCTTCT ACCGCTCTAG CTATCCACAG CAGCAGGGCC TGGGTGCCTC CTACAGGACA 
GAGTCGGCAC AGCGGCAAGC TTGCATGTAT GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG 
CCCAGCCTAG AGGACATCAG CTGCAACACG TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC 
ACCGTCACCA CCGTGCAGCC CATGGACAGG CTACCCTACC AGCACTTCTC CGCTCACTTC 
ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT GGCATGGCCA ACCATGGCTC CCCACAGCTG 
GGAGAGGGAA TGTTCCAGCA CCAGACCTCC GTGGCCCACC AGCCTGTGGT CAGGCAGTGT 
GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC ACCCTTCAGC CCCCTGAGTT CCTCTACTCT 
CATGGCGTGC CAAGGACTCT ATCCCCTCAT CAGTACCACT CTGTGCACGG AGTTGGCATG 
GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542448.1
CDS	203..1609
Translation	MEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRALSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKEEECSTTDHPYKKPYMETSPSEEDSFYRSSYPQQQGLGASYRTESAQRQACMYASSAPPSEPVPSLEDISCNTWPSMPSYSSCTVTTVQPMDRLPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPGTLQPPEFLYSHGVPRTLSPHQYHSVHGVGMVPEWSDNS

Target ORF information:

RefSeq Version	NM_080717.3
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box transcription factor 5 (TBX5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080717.3

ORF Insert Sequence:

ATGGAGGGAA TCAAAGTGTT TCTCCATGAA AGAGAACTGT GGCTAAAATT CCACGAAGTG 
GGCACGGAAA TGATCATAAC CAAGGCTGGA AGGCGGATGT TTCCCAGTTA CAAAGTGAAG 
GTGACGGGCC TTAATCCCAA AACGAAGTAC ATTCTTCTCA TGGACATTGT ACCTGCCGAC 
GATCACAGAT ACAAATTCGC AGATAATAAA TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC 
ATGCCTGGCC GCCTGTACGT GCACCCAGAC TCCCCCGCCA CCGGGGCGCA TTGGATGAGG 
CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC ACCAACAACC ACCTGGACCC ATTTGGGCAT 
ATTATTCTAA ATTCCATGCA CAAATACCAG CCTAGATTAC ACATCGTGAA AGCGGATGAA 
AATAATGGAT TTGGCTCAAA AAATACAGCG TTCTGCACTC ACGTCTTTCC TGAGACTGCG 
TTTATAGCAG TGACTTCCTA CCAGAACCAC AAGATCACGC AATTAAAGAT TGAGAATAAT 
CCCTTTGCCA AAGGATTTCG GGGCAGTGAT GACATGGAGC TGCACAGAAT GTCAAGAATG 
CAAAGTAAAG AATATCCCGT GGTCCCCAGG AGCACCGTGA GGCAAAAAGT GGCCTCCAAC 
CACAGTCCTT TCAGCAGCGA GTCTCGAGCT CTCTCCACCT CATCCAATTT GGGGTCCCAA 
TACCAGTGTG AGAATGGTGT TTCCGGCCCC TCCCAGGACC TCCTGCCTCC ACCCAACCCA 
TACCCACTGC CCCAGGAGCA TAGCCAAATT TACCATTGTA CCAAGAGGAA AGAGGAAGAA 
TGTTCCACCA CAGACCATCC CTATAAGAAG CCCTACATGG AGACATCACC CAGTGAAGAA 
GATTCCTTCT ACCGCTCTAG CTATCCACAG CAGCAGGGCC TGGGTGCCTC CTACAGGACA 
GAGTCGGCAC AGCGGCAAGC TTGCATGTAT GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG 
CCCAGCCTAG AGGACATCAG CTGCAACACG TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC 
ACCGTCACCA CCGTGCAGCC CATGGACAGG CTACCCTACC AGCACTTCTC CGCTCACTTC 
ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT GGCATGGCCA ACCATGGCTC CCCACAGCTG 
GGAGAGGGAA TGTTCCAGCA CCAGACCTCC GTGGCCCACC AGCCTGTGGT CAGGCAGTGT 
GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC ACCCTTCAGC CCCCTGAGTT CCTCTACTCT 
CATGGCGTGC CAAGGACTCT ATCCCCTCAT CAGTACCACT CTGTGCACGG AGTTGGCATG 
GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBX5 ( XM_017019912.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875401.1 Homo sapiens T-box transcription factor 5 (TBX5), transcript variant X1, mRNA.

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CloneID	OHu84128
Clone ID Related Accession (Same CDS sequence)	XM_017019912.1
Accession Version	XM_017019912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	T-box transcription factor TBX5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGAG AGCAGAACCT TGCGCGGGCA CAGGGCCCTG GGCGCACCAT GGCCGACGCA 
GACGAGGGCT TTGGCCTGGC GCACACGCCT CTGGAGCCTG ACGCAAAAGA CCTGCCCTGC 
GATTCGAAAC CCGAGAGCGC GCTCGGGGCC CCCAGCAAGT CCCCGTCGTC CCCGCAGGCC 
GCCTTCACCC AGCAGGGCAT GGAGGGAATC AAAGTGTTTC TCCATGAAAG AGAACTGTGG 
CTAAAATTCC ACGAAGTGGG CACGGAAATG ATCATAACCA AGGCTGGAAG GCGGATGTTT 
CCCAGTTACA AAGTGAAGGT GACGGGCCTT AATCCCAAAA CGAAGTACAT TCTTCTCATG 
GACATTGTAC CTGCCGACGA TCACAGATAC AAATTCGCAG ATAATAAATG GTCTGTGACG 
GGCAAAGCTG AGCCCGCCAT GCCTGGCCGC CTGTACGTGC ACCCAGACTC CCCCGCCACC 
GGGGCGCATT GGATGAGGCA GCTCGTCTCC TTCCAGAAAC TCAAGCTCAC CAACAACCAC 
CTGGACCCAT TTGGGCATAT TATTCTAAAT TCCATGCACA AATACCAGCC TAGATTACAC 
ATCGTGAAAG CGGATGAAAA TAATGGATTT GGCTCAAAAA ATACAGCGTT CTGCACTCAC 
GTCTTTCCTG AGACTGCGTT TATAGCAGTG ACTTCCTACC AGAACCACAA GATCACGCAA 
TTAAAGATTG AGAATAATCC CTTTGCCAAA GGATTTCGGG GCAGTGATGA CATGGAGCTG 
CACAGAATGT CAAGAATGCA AAGTAAAGAA TATCCCGTGG TCCCCAGGAG CACCGTGAGG 
CAAAAAGTGG CCTCCAACCA CAGTCCTTTC AGCAGCGAGT CTCGAGCTCT CTCCACCTCA 
TCCAATTTGG GGTCCCAATA CCAGTGTGAG AATGGTGTTT CCGGCCCCTC CCAGGACCTC 
CTGCCTCCAC CCAACCCATA CCCACTGCCC CAGGAGCATA GCCAAATTTA CCATTGTACC 
AAGAGGAAAG AGGAAGAATG TTCCACCACA GACCATCCCT ATAAGAAGCC CTACATGGAG 
ACATCACCCA GTGAAGAAGA TTCCTTCTAC CGCTCTAGCT ATCCACAGCA GCAGGGCCTG 
GGTGCCTCCT ACAGGACAGA GTCGGCACAG CGGCAAGCTT GCATGTATGC CAGCTCTGCG 
CCCCCCAGCG AGCCTGTGCC CAGCCTAGAG GACATCAGCT GCAACACGTG GCCAAGCATG 
CCTTCCTACA GCAGCTGCAC CGTCACCACC GTGCAGCCCA TGGACAGGCT ACCCTACCAG 
CACTTCTCCG CTCACTTCAC CTCGGGGCCC CTGGTCCCTC GGCTGGCTGG CATGGCCAAC 
CATGGCTCCC CACAGCTGGG AGAGGGAATG TTCCAGCACC AGACCTCCGT GGCCCACCAG 
CCTGTGGTCA GGCAGTGTGG GCCTCAGACT GGCCTGCAGT CCCCTGGCAC CCTTCAGCCC 
CCTGAGTTCC TCTACTCTCA TGGCGTGCCA AGGACTCTAT CCCCTCATCA GTACCACTCT 
GTGCACGGAG TTGGCATGGT GCCAGAGTGG AGCGACAATA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875401.1
CDS	2371..3975
Translation	MDGEQNLARAQGPGRTMADADEGFGLAHTPLEPDAKDLPCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRALSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKEEECSTTDHPYKKPYMETSPSEEDSFYRSSYPQQQGLGASYRTESAQRQACMYASSAPPSEPVPSLEDISCNTWPSMPSYSSCTVTTVQPMDRLPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPGTLQPPEFLYSHGVPRTLSPHQYHSVHGVGMVPEWSDNS

Target ORF information:

RefSeq Version	XM_017019912.1
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box transcription factor 5 (TBX5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019912.1

ORF Insert Sequence:

ATGGATGGAG AGCAGAACCT TGCGCGGGCA CAGGGCCCTG GGCGCACCAT GGCCGACGCA 
GACGAGGGCT TTGGCCTGGC GCACACGCCT CTGGAGCCTG ACGCAAAAGA CCTGCCCTGC 
GATTCGAAAC CCGAGAGCGC GCTCGGGGCC CCCAGCAAGT CCCCGTCGTC CCCGCAGGCC 
GCCTTCACCC AGCAGGGCAT GGAGGGAATC AAAGTGTTTC TCCATGAAAG AGAACTGTGG 
CTAAAATTCC ACGAAGTGGG CACGGAAATG ATCATAACCA AGGCTGGAAG GCGGATGTTT 
CCCAGTTACA AAGTGAAGGT GACGGGCCTT AATCCCAAAA CGAAGTACAT TCTTCTCATG 
GACATTGTAC CTGCCGACGA TCACAGATAC AAATTCGCAG ATAATAAATG GTCTGTGACG 
GGCAAAGCTG AGCCCGCCAT GCCTGGCCGC CTGTACGTGC ACCCAGACTC CCCCGCCACC 
GGGGCGCATT GGATGAGGCA GCTCGTCTCC TTCCAGAAAC TCAAGCTCAC CAACAACCAC 
CTGGACCCAT TTGGGCATAT TATTCTAAAT TCCATGCACA AATACCAGCC TAGATTACAC 
ATCGTGAAAG CGGATGAAAA TAATGGATTT GGCTCAAAAA ATACAGCGTT CTGCACTCAC 
GTCTTTCCTG AGACTGCGTT TATAGCAGTG ACTTCCTACC AGAACCACAA GATCACGCAA 
TTAAAGATTG AGAATAATCC CTTTGCCAAA GGATTTCGGG GCAGTGATGA CATGGAGCTG 
CACAGAATGT CAAGAATGCA AAGTAAAGAA TATCCCGTGG TCCCCAGGAG CACCGTGAGG 
CAAAAAGTGG CCTCCAACCA CAGTCCTTTC AGCAGCGAGT CTCGAGCTCT CTCCACCTCA 
TCCAATTTGG GGTCCCAATA CCAGTGTGAG AATGGTGTTT CCGGCCCCTC CCAGGACCTC 
CTGCCTCCAC CCAACCCATA CCCACTGCCC CAGGAGCATA GCCAAATTTA CCATTGTACC 
AAGAGGAAAG AGGAAGAATG TTCCACCACA GACCATCCCT ATAAGAAGCC CTACATGGAG 
ACATCACCCA GTGAAGAAGA TTCCTTCTAC CGCTCTAGCT ATCCACAGCA GCAGGGCCTG 
GGTGCCTCCT ACAGGACAGA GTCGGCACAG CGGCAAGCTT GCATGTATGC CAGCTCTGCG 
CCCCCCAGCG AGCCTGTGCC CAGCCTAGAG GACATCAGCT GCAACACGTG GCCAAGCATG 
CCTTCCTACA GCAGCTGCAC CGTCACCACC GTGCAGCCCA TGGACAGGCT ACCCTACCAG 
CACTTCTCCG CTCACTTCAC CTCGGGGCCC CTGGTCCCTC GGCTGGCTGG CATGGCCAAC 
CATGGCTCCC CACAGCTGGG AGAGGGAATG TTCCAGCACC AGACCTCCGT GGCCCACCAG 
CCTGTGGTCA GGCAGTGTGG GCCTCAGACT GGCCTGCAGT CCCCTGGCAC CCTTCAGCCC 
CCTGAGTTCC TCTACTCTCA TGGCGTGCCA AGGACTCTAT CCCCTCATCA GTACCACTCT 
GTGCACGGAG TTGGCATGGT GCCAGAGTGG AGCGACAATA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Downregulation of NFAT3 Due to Lack of T-Box Transcription Factor TBX5 Is Crucial for Cytokine Expression in T Cells.
Journal of immunology (Baltimore, Md. : 1950)200(1)92-100(2018 Jan)
Kaminuma O,Kitamura N,Nishito Y,Nemoto S,Tatsumi H,Mori A,Hiroi T

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TBX5 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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