SPAM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAM1
Entrez Gene ID	6677
Full Name	sperm adhesion molecule 1
Synonyms	HEL-S-96n,HYA1,HYAL1,HYAL3,HYAL5,PH-20,PH20,SPAG15
General protein information	Preferred Names sperm adhesion molecule 1 Names hyaluronidase PH-20 PH-20 hyaluronidase epididymis secretory sperm binding protein Li 96n hyal-PH20 hyaluronoglucosaminidase PH-20 sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) sperm surface protein PH-20
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q31.32
Summary	Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010].
Disorder MIM:	600930

mRNA and Protein(s)

mRNA	Protein	Name
NM_003117.4	NP_003108.2	hyaluronidase PH-20 isoform 1
NM_001174044.1	NP_001167515.1	hyaluronidase PH-20 isoform 2
NM_001174044.2	NP_001167515.1	hyaluronidase PH-20 isoform 2
NM_001174046.1	NP_001167517.1	hyaluronidase PH-20 isoform 2
NM_153189.3	NP_694859.1	hyaluronidase PH-20 isoform 2
NM_003117.5	NP_003108.2	hyaluronidase PH-20 isoform 1
NM_153189.2	NP_694859.1	hyaluronidase PH-20 isoform 2
NM_001174045.1	NP_001167516.1	hyaluronidase PH-20 isoform 2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100486024	XP_002932242.2
Canis lupus familiaris (dog)	SPAM1	XP_005628499.1
Gallus gallus (chicken)	SPAM1	XP_004937576.1
	SPAM1	XP_001086882.2
Mus musculus (house mouse)	Hyal5	NP_083233.1
Pan troglodytes (chimpanzee)	SPAM1	XP_003318800.1
Bos taurus (cattle)	SPAM1	NP_001008413.3
Homo sapiens (human)	SPAM1	NP_694859.1
Bos taurus (cattle)	LOC509761	XP_002686960.1
Rattus norvegicus (Norway rat)	Hyal5	NP_001019492.1
Danio rerio (zebrafish)	spam1	NP_001074154.1

Gene Ontology
Bibliography

Related articles in PubMed

Discovery of human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes and their effects on fertility in male and female mice.
Chen X, Liu X, Ren X, Li X, Wang L, Zang W
Reproduction, fertility, and development28(4)416-27(2016 Mar)

Recombinant human hyaluronidase PH20 does not stimulate an acute inflammatory response and inhibits lipopolysaccharide-induced neutrophil recruitment in the air pouch model of inflammation.
Huang Z, Zhao C, Chen Y, Cowell JA, Wei G, Kultti A, Huang L, Thompson CB, Rosengren S, Frost GI, Shepard HM
Journal of immunology (Baltimore, Md. : 1950)192(11)5285-95(2014 Jun)

Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition.
Redgrove KA, Anderson AL, McLaughlin EA, O'Bryan MK, Aitken RJ, Nixon B
Molecular human reproduction19(3)120-35(2013 Mar)

Digestion products of the PH20 hyaluronidase inhibit remyelination.
Preston M, Gong X, Su W, Matsumoto SG, Banine F, Winkler C, Foster S, Xing R, Struve J, Dean J, Baggenstoss B, Weigel PH, Montine TJ, Back SA, Sherman LS
Annals of neurology73(2)266-80(2013 Feb)

The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition.
Redgrove KA, Nixon B, Baker MA, Hetherington L, Baker G, Liu DY, Aitken RJ
PloS one7(11)e50851(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes reduced fertility in male/female mice.
Title: Discovery of human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes and their effects on fertility in male and female mice.

results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch.
Title: Recombinant human hyaluronidase PH20 does not stimulate an acute inflammatory response and inhibits lipopolysaccharide-induced neutrophil recruitment in the air pouch model of inflammation.

HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition, such as arylsulfatase A and SPAM1.
Title: Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition.

PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit oligodendrocyte precursor cell maturation and remyelination.
Title: Digestion products of the PH20 hyaluronidase inhibit remyelination.

The interaction between SPAM1, ARSA and HSPA2 in a multimeric complex mediating sperm-egg interaction.
Title: The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition.

The following SPAM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09380	NM_003117.5 Latest version!	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu09309	NM_001174044.2 Latest version!	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OHu09309	NM_001174045.1 Latest version!	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OHu09309	NM_153189.3 Latest version!	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OHu09309	NM_001174046.1 Latest version!	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
View Old Accession Versions >>
OHu09309	NM_001174044.1	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OHu09380	NM_003117.4	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu09309	NM_153189.2	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAM1 ( NM_003117.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003108.2 Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09380
Clone ID Related Accession (Same CDS sequence)	NM_003117.4 , NM_003117.5
Accession Version	NM_003117.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	hyaluronidase PH-20 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY920279.1, AK292229.1, BC026163.1 and AC004690.2. On Jun 2, 2019 this sequence version replaced NM_003117.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026163.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2148093 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTTGGAG GCTGGAAGTC 
TGGGATCAAG GTATTAGCAG AATTGGTTTC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003108.2
CDS	431..1966
Translation	MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIWRLEVWDQGISRIGFF

Target ORF information:

RefSeq Version	NM_003117.5
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003117.5

ORF Insert Sequence:

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTTGGAG GCTGGAAGTC 
TGGGATCAAG GTATTAGCAG AATTGGTTTC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAM1 ( NM_001174044.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001167515.1 Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09309
Clone ID Related Accession (Same CDS sequence)	NM_001174044.1 , NM_001174044.2 , NM_001174046.1 , NM_153189.3 , NM_153189.2 , NM_001174045.1
Accession Version	NM_001174044.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	hyaluronidase PH-20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB023094.1, AY920278.1 and X84347.1. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1, resulting in a shorter and distinct C-terminus (isoform 2). Variants 2-5 all encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY920278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTGTTAG TATTTTGTTT 
CTTATCATTT CTTCTGTAGC GAGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001167515.1
CDS	619..2148
Translation	MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIVSILFLIISSVASL

Target ORF information:

RefSeq Version	NM_001174044.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001174044.1

ORF Insert Sequence:

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTGTTAG TATTTTGTTT 
CTTATCATTT CTTCTGTAGC GAGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAM1 ( NM_001174044.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001167515.1 Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09309
Clone ID Related Accession (Same CDS sequence)	NM_001174044.1 , NM_001174044.2 , NM_001174046.1 , NM_153189.3 , NM_153189.2 , NM_001174045.1
Accession Version	NM_001174044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	hyaluronidase PH-20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY920278.1 and X84347.1. On Jun 2, 2019 this sequence version replaced NM_001174044.1. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1, resulting in a shorter and distinct C-terminus (isoform 2). Variants 2-5 all encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY920278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTGTTAG TATTTTGTTT 
CTTATCATTT CTTCTGTAGC GAGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001167515.1
CDS	610..2139
Translation	MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIVSILFLIISSVASL

Target ORF information:

RefSeq Version	NM_001174044.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001174044.2

ORF Insert Sequence:

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTGTTAG TATTTTGTTT 
CTTATCATTT CTTCTGTAGC GAGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAM1 ( NM_001174046.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001167517.1 Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu09309
Clone ID Related Accession (Same CDS sequence)	NM_001174044.1 , NM_001174044.2 , NM_001174046.1 , NM_153189.3 , NM_153189.2 , NM_001174045.1
Accession Version	NM_001174046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	hyaluronidase PH-20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292229.1, AY920281.1 and X84347.1. Transcript Variant: This variant (5) differs in the 5' UTR and uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1, resulting in a shorter and distinct C-terminus (isoform 2). Variants 2-5 all encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292229.1, AY920281.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTGTTAG TATTTTGTTT 
CTTATCATTT CTTCTGTAGC GAGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001167517.1
CDS	529..2058
Translation	MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIVSILFLIISSVASL

Target ORF information:

RefSeq Version	NM_001174046.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001174046.1

ORF Insert Sequence:

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTGTTAG TATTTTGTTT 
CTTATCATTT CTTCTGTAGC GAGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAM1 ( NM_153189.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_694859.1 Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09309
Clone ID Related Accession (Same CDS sequence)	NM_001174044.1 , NM_001174044.2 , NM_001174046.1 , NM_153189.3 , NM_153189.2 , NM_001174045.1
Accession Version	NM_153189.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-08
Organism	Homo sapiens(human)
Product	hyaluronidase PH-20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY920279.1 and X84347.1. On Jun 2, 2019 this sequence version replaced NM_153189.2. Transcript Variant: This variant (2) uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1, resulting in a shorter and distinct C-terminus (isoform 2). Variants 2-5 all encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY920279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTGTTAG TATTTTGTTT 
CTTATCATTT CTTCTGTAGC GAGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694859.1
CDS	431..1960
Translation	MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIVSILFLIISSVASL

Target ORF information:

RefSeq Version	NM_153189.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153189.3

ORF Insert Sequence:

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTGTTAG TATTTTGTTT 
CTTATCATTT CTTCTGTAGC GAGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAM1 ( NM_153189.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_694859.1 Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09309
Clone ID Related Accession (Same CDS sequence)	NM_001174044.1 , NM_001174044.2 , NM_001174046.1 , NM_153189.3 , NM_153189.2 , NM_001174045.1
Accession Version	NM_153189.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	hyaluronidase PH-20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB023094.1, AY920279.1 and X84347.1. On Mar 20, 2010 this sequence version replaced NM_153189.1. Transcript Variant: This variant (2) uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1, resulting in a shorter and distinct C-terminus (isoform 2). Variants 2-5 all encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY920279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTGTTAG TATTTTGTTT 
CTTATCATTT CTTCTGTAGC GAGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694859.1
CDS	440..1969
Translation	MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIVSILFLIISSVASL

Target ORF information:

RefSeq Version	NM_153189.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153189.2

ORF Insert Sequence:

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTGTTAG TATTTTGTTT 
CTTATCATTT CTTCTGTAGC GAGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAM1 ( NM_001174045.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001167516.1 Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 4, mRNA.

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CloneID	OHu09309
Clone ID Related Accession (Same CDS sequence)	NM_001174044.1 , NM_001174044.2 , NM_001174046.1 , NM_153189.3 , NM_153189.2 , NM_001174045.1
Accession Version	NM_001174045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	hyaluronidase PH-20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292229.1, AY920280.1 and X84347.1. Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1, resulting in a shorter and distinct C-terminus (isoform 2). Variants 2-5 all encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY920280.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTGTTAG TATTTTGTTT 
CTTATCATTT CTTCTGTAGC GAGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001167516.1
CDS	614..2143
Translation	MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIVSILFLIISSVASL

Target ORF information:

RefSeq Version	NM_001174045.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001174045.1

ORF Insert Sequence:

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTGTTAG TATTTTGTTT 
CTTATCATTT CTTCTGTAGC GAGTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAM1 ( NM_003117.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003108.2 Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 1, mRNA.

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CloneID	OHu09380
Clone ID Related Accession (Same CDS sequence)	NM_003117.4 , NM_003117.5
Accession Version	NM_003117.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	hyaluronidase PH-20 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB023094.1, AY920279.1, AK292229.1 and BC026163.1. On Mar 20, 2010 this sequence version replaced NM_003117.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026163.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2148093 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTTGGAG GCTGGAAGTC 
TGGGATCAAG GTATTAGCAG AATTGGTTTC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003108.2
CDS	440..1975
Translation	MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIWRLEVWDQGISRIGFF

Target ORF information:

RefSeq Version	NM_003117.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm adhesion molecule 1 (SPAM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003117.4

ORF Insert Sequence:

ATGGGAGTGC TAAAATTCAA GCACATCTTT TTCAGAAGCT TTGTTAAATC AAGTGGAGTA 
TCCCAGATAG TTTTCACCTT CCTTCTGATT CCATGTTGCT TGACTCTGAA TTTCAGAGCA 
CCTCCTGTTA TTCCAAATGT GCCTTTCCTC TGGGCCTGGA ATGCCCCAAG TGAATTTTGT 
CTTGGAAAAT TTGATGAGCC ACTAGATATG AGCCTCTTCT CTTTCATAGG AAGCCCCCGA 
ATAAACGCCA CCGGGCAAGG TGTTACAATA TTTTATGTTG ATAGACTTGG CTACTATCCT 
TACATAGATT CAATCACAGG AGTAACTGTG AATGGAGGAA TCCCCCAGAA GATTTCCTTA 
CAAGACCATC TGGACAAAGC TAAGAAAGAC ATTACATTTT ATATGCCAGT AGACAATTTG 
GGAATGGCTG TTATTGACTG GGAAGAATGG AGACCCACTT GGGCAAGAAA CTGGAAACCT 
AAAGATGTTT ACAAGAATAG GTCTATTGAA TTGGTTCAGC AACAAAATGT ACAACTTAGT 
CTCACAGAGG CCACTGAGAA AGCAAAACAA GAATTTGAAA AGGCAGGGAA GGATTTCCTG 
GTAGAGACTA TAAAATTGGG AAAATTACTT CGGCCAAATC ACTTGTGGGG TTATTATCTT 
TTTCCGGATT GTTACAACCA TCACTATAAG AAACCCGGTT ACAATGGAAG TTGCTTCAAT 
GTAGAAATAA AAAGAAATGA TGATCTCAGC TGGTTGTGGA ATGAAAGCAC TGCTCTTTAC 
CCATCCATTT ATTTGAACAC TCAGCAGTCT CCTGTAGCTG CTACACTCTA TGTGCGCAAT 
CGAGTTCGGG AAGCCATCAG AGTTTCCAAA ATACCTGATG CAAAAAGTCC ACTTCCGGTT 
TTTGCATATA CCCGCATAGT TTTTACTGAT CAAGTTTTGA AATTCCTTTC TCAAGATGAA 
CTTGTGTATA CATTTGGCGA AACTGTTGCT CTGGGTGCTT CTGGAATTGT AATATGGGGA 
ACCCTCAGTA TAATGCGAAG TATGAAATCT TGCTTGCTCC TAGACAATTA CATGGAGACT 
ATACTGAATC CTTACATAAT CAACGTCACA CTAGCAGCCA AAATGTGTAG CCAAGTGCTT 
TGCCAGGAGC AAGGAGTGTG TATAAGGAAA AACTGGAATT CAAGTGACTA TCTTCACCTC 
AACCCAGATA ATTTTGCTAT TCAACTTGAG AAAGGTGGAA AGTTCACAGT ACGTGGAAAA 
CCGACACTTG AAGACCTGGA GCAATTTTCT GAAAAATTTT ATTGCAGCTG TTATAGCACC 
TTGAGTTGTA AGGAGAAAGC TGATGTAAAA GACACTGATG CTGTTGATGT GTGTATTGCT 
GATGGTGTCT GTATAGATGC TTTTCTAAAA CCTCCCATGG AGACAGAAGA ACCTCAAATT 
TTCTACAATG CTTCACCCTC CACACTATCT GCCACAATGT TCATTTGGAG GCTGGAAGTC 
TGGGATCAAG GTATTAGCAG AATTGGTTTC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Discovery of human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes and their effects on fertility in male and female mice.
Reproduction, fertility, and development28(4)416-27(2016 Mar)
Chen X,Liu X,Ren X,Li X,Wang L,Zang W

Recombinant human hyaluronidase PH20 does not stimulate an acute inflammatory response and inhibits lipopolysaccharide-induced neutrophil recruitment in the air pouch model of inflammation.
Journal of immunology (Baltimore, Md. : 1950)192(11)5285-95(2014 Jun)
Huang Z,Zhao C,Chen Y,Cowell JA,Wei G,Kultti A,Huang L,Thompson CB,Rosengren S,Frost GI,Shepard HM

Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition.
Molecular human reproduction19(3)120-35(2013 Mar)
Redgrove KA,Anderson AL,McLaughlin EA,O'Bryan MK,Aitken RJ,Nixon B

Digestion products of the PH20 hyaluronidase inhibit remyelination.
Annals of neurology73(2)266-80(2013 Feb)
Preston M,Gong X,Su W,Matsumoto SG,Banine F,Winkler C,Foster S,Xing R,Struve J,Dean J,Baggenstoss B,Weigel PH,Montine TJ,Back SA,Sherman LS

The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition.
PloS one7(11)e50851(2012)
Redgrove KA,Nixon B,Baker MA,Hetherington L,Baker G,Liu DY,Aitken RJ

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SPAM1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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