SPAM1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAM1
Entrez Gene ID	353352
Full Name	sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
Synonyms	PH-20
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005205627.4	XP_005205684.1	sperm adhesion molecule 1 isoform X1
XM_024990502.1	XP_024846270.1	sperm adhesion molecule 1 isoform X2
NM_001008413.3	NP_001008413.3	sperm adhesion molecule 1 precursor
XM_024990500.1	XP_024846268.1	sperm adhesion molecule 1 isoform X1
XM_024990503.1	XP_024846271.1	sperm adhesion molecule 1 isoform X2
XM_005205627.3	XP_005205684.1	sperm adhesion molecule 1 isoform X1
XM_024990501.1	XP_024846269.1	sperm adhesion molecule 1 isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100486024	XP_002932242.2
Canis lupus familiaris (dog)	SPAM1	XP_005628499.1
Gallus gallus (chicken)	SPAM1	XP_004937576.1
	SPAM1	XP_001086882.2
Mus musculus (house mouse)	Hyal5	NP_083233.1
Pan troglodytes (chimpanzee)	SPAM1	XP_003318800.1
Bos taurus (cattle)	SPAM1	NP_001008413.3
Homo sapiens (human)	SPAM1	NP_694859.1
Bos taurus (cattle)	LOC509761	XP_002686960.1
Rattus norvegicus (Norway rat)	Hyal5	NP_001019492.1
Danio rerio (zebrafish)	spam1	NP_001074154.1

Gene Ontology
Bibliography

Related articles in PubMed

Stratification of chondroitin sulfate binding sites in 3D-model of bovine testicular hyaluronidase and effective size of glycosaminoglycan coat of the modified protein.
Maksimenko AV, Turashev AD, Beabealashvili RS
Biochemistry. Biokhimiia80(3)284-95(2015 Mar)

Effects of divalent cations on bovine testicular hyaluronidase catalyzed transglycosylation of chondroitin sulfates.
Kakizaki I, Nukatsuka I, Takagaki K, Majima M, Iwafune M, Suto S, Endo M
Biochemical and biophysical research communications406(2)239-44(2011 Mar)

SPAM1 isoforms from two tissue origins are differentially localized within ejaculated bull sperm membranes and have different roles during fertilization.
Morin G, Sullivan R, Laflamme I, Robert C, Leclerc P
Biology of reproduction82(2)271-81(2010 Feb)

Development of a fluorescent substrate to measure hyaluronidase activity.
Zhang LS, Mummert ME
Analytical biochemistry379(1)80-5(2008 Aug)

Chain-length dependence of the kinetics of the hyaluronan hydrolysis catalyzed by bovine testicular hyaluronidase.
Deschrevel B, Tranchepain F, Vincent JC
Matrix biology : journal of the International Society for Matrix Biology27(5)475-86(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bovine testicular hyaluronidase 3D-model demonstrated lysine residue stratification during enzyme modification.
Title: Stratification of chondroitin sulfate binding sites in 3D-model of bovine testicular hyaluronidase and effective size of glycosaminoglycan coat of the modified protein.

the effect of metal cations on the transglycosylation by bovine testicular hyaluronidase using as substrates metal salts of glycosaminoglycan chains was investigated.
Title: Effects of divalent cations on bovine testicular hyaluronidase catalyzed transglycosylation of chondroitin sulfates.

results clearly demonstrate that ejaculated bull sperm possess two forms of sperm adhesion molecule 1(SPAM1): one (epididymal) expressed at the surface, and one (testicular) that interacts with the zona pellucida after the acrosome exocytosis.
Title: SPAM1 isoforms from two tissue origins are differentially localized within ejaculated bull sperm membranes and have different roles during fertilization.

The simplicity, sensitivity, and versatility of the fluorescent substrate (termed FRET-HA) suggest that it will have utility in a variety of assay platforms and should be a new tool for assessing hyaluronidase activity.
Title: Development of a fluorescent substrate to measure hyaluronidase activity.

The hydrolysis Vm values strongly suggest that as the chain length decreases the hyaluronidase increasingly catalyses transglycosylation rather than hydrolysis.
Title: Chain-length dependence of the kinetics of the hyaluronan hydrolysis catalyzed by bovine testicular hyaluronidase.

The following SPAM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa18710	NM_001008413.3 Latest version!	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa18710	XM_005205627.4 Latest version!	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa18710	XM_024990501.1 Latest version!	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa18710	XM_024990500.1 Latest version!	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa18711	XM_024990502.1 Latest version!	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBa18711	XM_024990503.1 Latest version!	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OBa18710	XM_005205627.3	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAM1 ( NM_001008413.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001008413.3 Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), mRNA.

Price & Availability↑

CloneID	OBa18710
Clone ID Related Accession (Same CDS sequence)	XM_005205627.4 , NM_001008413.3 , XM_024990500.1 , XM_005205627.3 , XM_024990501.1
Accession Version	NM_001008413.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Bos taurus(cattle)
Product	sperm adhesion molecule 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000004.1. On Apr 14, 2011 this sequence version replaced NM_001008413.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC110183.1, SRR5571257.34852.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END##

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
GGTGACCTTG TGAATTCGGT TGGTGAGATC GTTTCTCTAG GTGCCTCTGG GATTATAATG 
TGGGGCAGTC TCAATCTAAG CTTATCTATG CAATCTTGCA TGAACCTAGG CACTTACTTG 
AACACTACAC TGAATCCTTA CATAATCAAC GTCACCCTAG CCGCCAAAAT GTGCAGCCAA 
GTGCTTTGCC ACAATGAAGG AGTGTGTACA AGGAAACACT GGAATTCAAG CGACTATCTT 
CACCTGAACC CAATGAATTT TGCTATTCAA ACTGGGGAAG GTGGAAAATA CACAGTACCT 
GGGACAGTCA CACTTGAAGA CTTGCAAAAG TTTTCTGATA CATTTTATTG CAGTTGTTAT 
GCCAACATCC ACTGTAAGAA GAGAGTTGAT ATAAAAAATG TTCATAGTGT TAACGTGTGT 
ATGGCAGAAG ACATTTGTAT AGACAGCCCT GTGAAGTTAC AACCCAGTGA TCATTCCTCC 
AGCCAGGAGG CATCTACTAC CACCTTCAGC AGTATCTCAC CCTCCACTAC AACTGCCACA 
GTATCTCCAT GTACTCCTGA GAAACACTCC CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA 
GTCATCCCCA ACGTCACCCA AAAGGCGTGT CAAAGTGTTA AATTGAAGAA CATTTCCTAT 
CAGTCACCTA TTCAAAATAT TAAAAATCAA ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008413.3
CDS	297..1958
Translation	MRMLRRHHISFRSFAGSSGTPQAVFTFLLLPCCLALDFRAPPLISNTSFLWAWNAPVERCVNRRFQLPPDLRLFSVKGSPQKSATGQFITLFYADRLGYYPHIDEKTGKTVFGGIPQLGNLKSHLEKAKNDIAYYIPNDSVGLAVIDWENWRPTWARNWKPKDVYRDESVELVLQKNPQLSFPEASKIAKVDFETAGKSFMQETLKLGKLLRPNHLWGYYLFPDCYNHNHNQPTYNGNCPDVEKRRNDDLEWLWKESTALFPSVYLNIRLKSTQNAALYVRNRVQEAIRLSKIASVESPLPVFVYARPVFTDGSSTYLSQGDLVNSVGEIVSLGASGIIMWGSLNLSLSMQSCMNLGTYLNTTLNPYIINVTLAAKMCSQVLCHNEGVCTRKHWNSSDYLHLNPMNFAIQTGEGGKYTVPGTVTLEDLQKFSDTFYCSCYANIHCKKRVDIKNVHSVNVCMAEDICIDSPVKLQPSDHSSSQEASTTTFSSISPSTTTATVSPCTPEKHSPECLKVRCSEVIPNVTQKACQSVKLKNISYQSPIQNIKNQTTY

Target ORF information:

RefSeq Version	NM_001008413.3
Organism	Bos taurus(cattle)
Definition	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008413.3

ORF Insert Sequence:

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
GGTGACCTTG TGAATTCGGT TGGTGAGATC GTTTCTCTAG GTGCCTCTGG GATTATAATG 
TGGGGCAGTC TCAATCTAAG CTTATCTATG CAATCTTGCA TGAACCTAGG CACTTACTTG 
AACACTACAC TGAATCCTTA CATAATCAAC GTCACCCTAG CCGCCAAAAT GTGCAGCCAA 
GTGCTTTGCC ACAATGAAGG AGTGTGTACA AGGAAACACT GGAATTCAAG CGACTATCTT 
CACCTGAACC CAATGAATTT TGCTATTCAA ACTGGGGAAG GTGGAAAATA CACAGTACCT 
GGGACAGTCA CACTTGAAGA CTTGCAAAAG TTTTCTGATA CATTTTATTG CAGTTGTTAT 
GCCAACATCC ACTGTAAGAA GAGAGTTGAT ATAAAAAATG TTCATAGTGT TAACGTGTGT 
ATGGCAGAAG ACATTTGTAT AGACAGCCCT GTGAAGTTAC AACCCAGTGA TCATTCCTCC 
AGCCAGGAGG CATCTACTAC CACCTTCAGC AGTATCTCAC CCTCCACTAC AACTGCCACA 
GTATCTCCAT GTACTCCTGA GAAACACTCC CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA 
GTCATCCCCA ACGTCACCCA AAAGGCGTGT CAAAGTGTTA AATTGAAGAA CATTTCCTAT 
CAGTCACCTA TTCAAAATAT TAAAAATCAA ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAM1 ( XM_005205627.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205684.1 Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa18710
Clone ID Related Accession (Same CDS sequence)	XM_005205627.4 , NM_001008413.3 , XM_024990500.1 , XM_005205627.3 , XM_024990501.1
Accession Version	XM_005205627.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sperm adhesion molecule 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205627.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
GGTGACCTTG TGAATTCGGT TGGTGAGATC GTTTCTCTAG GTGCCTCTGG GATTATAATG 
TGGGGCAGTC TCAATCTAAG CTTATCTATG CAATCTTGCA TGAACCTAGG CACTTACTTG 
AACACTACAC TGAATCCTTA CATAATCAAC GTCACCCTAG CCGCCAAAAT GTGCAGCCAA 
GTGCTTTGCC ACAATGAAGG AGTGTGTACA AGGAAACACT GGAATTCAAG CGACTATCTT 
CACCTGAACC CAATGAATTT TGCTATTCAA ACTGGGGAAG GTGGAAAATA CACAGTACCT 
GGGACAGTCA CACTTGAAGA CTTGCAAAAG TTTTCTGATA CATTTTATTG CAGTTGTTAT 
GCCAACATCC ACTGTAAGAA GAGAGTTGAT ATAAAAAATG TTCATAGTGT TAACGTGTGT 
ATGGCAGAAG ACATTTGTAT AGACAGCCCT GTGAAGTTAC AACCCAGTGA TCATTCCTCC 
AGCCAGGAGG CATCTACTAC CACCTTCAGC AGTATCTCAC CCTCCACTAC AACTGCCACA 
GTATCTCCAT GTACTCCTGA GAAACACTCC CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA 
GTCATCCCCA ACGTCACCCA AAAGGCGTGT CAAAGTGTTA AATTGAAGAA CATTTCCTAT 
CAGTCACCTA TTCAAAATAT TAAAAATCAA ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205684.1
CDS	2597..4258
Translation	MRMLRRHHISFRSFAGSSGTPQAVFTFLLLPCCLALDFRAPPLISNTSFLWAWNAPVERCVNRRFQLPPDLRLFSVKGSPQKSATGQFITLFYADRLGYYPHIDEKTGKTVFGGIPQLGNLKSHLEKAKNDIAYYIPNDSVGLAVIDWENWRPTWARNWKPKDVYRDESVELVLQKNPQLSFPEASKIAKVDFETAGKSFMQETLKLGKLLRPNHLWGYYLFPDCYNHNHNQPTYNGNCPDVEKRRNDDLEWLWKESTALFPSVYLNIRLKSTQNAALYVRNRVQEAIRLSKIASVESPLPVFVYARPVFTDGSSTYLSQGDLVNSVGEIVSLGASGIIMWGSLNLSLSMQSCMNLGTYLNTTLNPYIINVTLAAKMCSQVLCHNEGVCTRKHWNSSDYLHLNPMNFAIQTGEGGKYTVPGTVTLEDLQKFSDTFYCSCYANIHCKKRVDIKNVHSVNVCMAEDICIDSPVKLQPSDHSSSQEASTTTFSSISPSTTTATVSPCTPEKHSPECLKVRCSEVIPNVTQKACQSVKLKNISYQSPIQNIKNQTTY

Target ORF information:

RefSeq Version	XM_005205627.4
Organism	Bos taurus(cattle)
Definition	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205627.4

ORF Insert Sequence:

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
GGTGACCTTG TGAATTCGGT TGGTGAGATC GTTTCTCTAG GTGCCTCTGG GATTATAATG 
TGGGGCAGTC TCAATCTAAG CTTATCTATG CAATCTTGCA TGAACCTAGG CACTTACTTG 
AACACTACAC TGAATCCTTA CATAATCAAC GTCACCCTAG CCGCCAAAAT GTGCAGCCAA 
GTGCTTTGCC ACAATGAAGG AGTGTGTACA AGGAAACACT GGAATTCAAG CGACTATCTT 
CACCTGAACC CAATGAATTT TGCTATTCAA ACTGGGGAAG GTGGAAAATA CACAGTACCT 
GGGACAGTCA CACTTGAAGA CTTGCAAAAG TTTTCTGATA CATTTTATTG CAGTTGTTAT 
GCCAACATCC ACTGTAAGAA GAGAGTTGAT ATAAAAAATG TTCATAGTGT TAACGTGTGT 
ATGGCAGAAG ACATTTGTAT AGACAGCCCT GTGAAGTTAC AACCCAGTGA TCATTCCTCC 
AGCCAGGAGG CATCTACTAC CACCTTCAGC AGTATCTCAC CCTCCACTAC AACTGCCACA 
GTATCTCCAT GTACTCCTGA GAAACACTCC CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA 
GTCATCCCCA ACGTCACCCA AAAGGCGTGT CAAAGTGTTA AATTGAAGAA CATTTCCTAT 
CAGTCACCTA TTCAAAATAT TAAAAATCAA ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAM1 ( XM_024990500.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846268.1 Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa18710
Clone ID Related Accession (Same CDS sequence)	XM_005205627.4 , NM_001008413.3 , XM_024990500.1 , XM_005205627.3 , XM_024990501.1
Accession Version	XM_024990500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sperm adhesion molecule 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
GGTGACCTTG TGAATTCGGT TGGTGAGATC GTTTCTCTAG GTGCCTCTGG GATTATAATG 
TGGGGCAGTC TCAATCTAAG CTTATCTATG CAATCTTGCA TGAACCTAGG CACTTACTTG 
AACACTACAC TGAATCCTTA CATAATCAAC GTCACCCTAG CCGCCAAAAT GTGCAGCCAA 
GTGCTTTGCC ACAATGAAGG AGTGTGTACA AGGAAACACT GGAATTCAAG CGACTATCTT 
CACCTGAACC CAATGAATTT TGCTATTCAA ACTGGGGAAG GTGGAAAATA CACAGTACCT 
GGGACAGTCA CACTTGAAGA CTTGCAAAAG TTTTCTGATA CATTTTATTG CAGTTGTTAT 
GCCAACATCC ACTGTAAGAA GAGAGTTGAT ATAAAAAATG TTCATAGTGT TAACGTGTGT 
ATGGCAGAAG ACATTTGTAT AGACAGCCCT GTGAAGTTAC AACCCAGTGA TCATTCCTCC 
AGCCAGGAGG CATCTACTAC CACCTTCAGC AGTATCTCAC CCTCCACTAC AACTGCCACA 
GTATCTCCAT GTACTCCTGA GAAACACTCC CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA 
GTCATCCCCA ACGTCACCCA AAAGGCGTGT CAAAGTGTTA AATTGAAGAA CATTTCCTAT 
CAGTCACCTA TTCAAAATAT TAAAAATCAA ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846268.1
CDS	2577..4238
Translation	MRMLRRHHISFRSFAGSSGTPQAVFTFLLLPCCLALDFRAPPLISNTSFLWAWNAPVERCVNRRFQLPPDLRLFSVKGSPQKSATGQFITLFYADRLGYYPHIDEKTGKTVFGGIPQLGNLKSHLEKAKNDIAYYIPNDSVGLAVIDWENWRPTWARNWKPKDVYRDESVELVLQKNPQLSFPEASKIAKVDFETAGKSFMQETLKLGKLLRPNHLWGYYLFPDCYNHNHNQPTYNGNCPDVEKRRNDDLEWLWKESTALFPSVYLNIRLKSTQNAALYVRNRVQEAIRLSKIASVESPLPVFVYARPVFTDGSSTYLSQGDLVNSVGEIVSLGASGIIMWGSLNLSLSMQSCMNLGTYLNTTLNPYIINVTLAAKMCSQVLCHNEGVCTRKHWNSSDYLHLNPMNFAIQTGEGGKYTVPGTVTLEDLQKFSDTFYCSCYANIHCKKRVDIKNVHSVNVCMAEDICIDSPVKLQPSDHSSSQEASTTTFSSISPSTTTATVSPCTPEKHSPECLKVRCSEVIPNVTQKACQSVKLKNISYQSPIQNIKNQTTY

Target ORF information:

RefSeq Version	XM_024990500.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990500.1

ORF Insert Sequence:

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
GGTGACCTTG TGAATTCGGT TGGTGAGATC GTTTCTCTAG GTGCCTCTGG GATTATAATG 
TGGGGCAGTC TCAATCTAAG CTTATCTATG CAATCTTGCA TGAACCTAGG CACTTACTTG 
AACACTACAC TGAATCCTTA CATAATCAAC GTCACCCTAG CCGCCAAAAT GTGCAGCCAA 
GTGCTTTGCC ACAATGAAGG AGTGTGTACA AGGAAACACT GGAATTCAAG CGACTATCTT 
CACCTGAACC CAATGAATTT TGCTATTCAA ACTGGGGAAG GTGGAAAATA CACAGTACCT 
GGGACAGTCA CACTTGAAGA CTTGCAAAAG TTTTCTGATA CATTTTATTG CAGTTGTTAT 
GCCAACATCC ACTGTAAGAA GAGAGTTGAT ATAAAAAATG TTCATAGTGT TAACGTGTGT 
ATGGCAGAAG ACATTTGTAT AGACAGCCCT GTGAAGTTAC AACCCAGTGA TCATTCCTCC 
AGCCAGGAGG CATCTACTAC CACCTTCAGC AGTATCTCAC CCTCCACTAC AACTGCCACA 
GTATCTCCAT GTACTCCTGA GAAACACTCC CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA 
GTCATCCCCA ACGTCACCCA AAAGGCGTGT CAAAGTGTTA AATTGAAGAA CATTTCCTAT 
CAGTCACCTA TTCAAAATAT TAAAAATCAA ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAM1 ( XM_005205627.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205684.1 Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa18710
Clone ID Related Accession (Same CDS sequence)	XM_005205627.4 , NM_001008413.3 , XM_024990500.1 , XM_005205627.3 , XM_024990501.1
Accession Version	XM_005205627.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sperm adhesion molecule 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103903.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205627.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
GGTGACCTTG TGAATTCGGT TGGTGAGATC GTTTCTCTAG GTGCCTCTGG GATTATAATG 
TGGGGCAGTC TCAATCTAAG CTTATCTATG CAATCTTGCA TGAACCTAGG CACTTACTTG 
AACACTACAC TGAATCCTTA CATAATCAAC GTCACCCTAG CCGCCAAAAT GTGCAGCCAA 
GTGCTTTGCC ACAATGAAGG AGTGTGTACA AGGAAACACT GGAATTCAAG CGACTATCTT 
CACCTGAACC CAATGAATTT TGCTATTCAA ACTGGGGAAG GTGGAAAATA CACAGTACCT 
GGGACAGTCA CACTTGAAGA CTTGCAAAAG TTTTCTGATA CATTTTATTG CAGTTGTTAT 
GCCAACATCC ACTGTAAGAA GAGAGTTGAT ATAAAAAATG TTCATAGTGT TAACGTGTGT 
ATGGCAGAAG ACATTTGTAT AGACAGCCCT GTGAAGTTAC AACCCAGTGA TCATTCCTCC 
AGCCAGGAGG CATCTACTAC CACCTTCAGC AGTATCTCAC CCTCCACTAC AACTGCCACA 
GTATCTCCAT GTACTCCTGA GAAACACTCC CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA 
GTCATCCCCA ACGTCACCCA AAAGGCGTGT CAAAGTGTTA AATTGAAGAA CATTTCCTAT 
CAGTCACCTA TTCAAAATAT TAAAAATCAA ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205684.1
CDS	2595..4256
Translation	MRMLRRHHISFRSFAGSSGTPQAVFTFLLLPCCLALDFRAPPLISNTSFLWAWNAPVERCVNRRFQLPPDLRLFSVKGSPQKSATGQFITLFYADRLGYYPHIDEKTGKTVFGGIPQLGNLKSHLEKAKNDIAYYIPNDSVGLAVIDWENWRPTWARNWKPKDVYRDESVELVLQKNPQLSFPEASKIAKVDFETAGKSFMQETLKLGKLLRPNHLWGYYLFPDCYNHNHNQPTYNGNCPDVEKRRNDDLEWLWKESTALFPSVYLNIRLKSTQNAALYVRNRVQEAIRLSKIASVESPLPVFVYARPVFTDGSSTYLSQGDLVNSVGEIVSLGASGIIMWGSLNLSLSMQSCMNLGTYLNTTLNPYIINVTLAAKMCSQVLCHNEGVCTRKHWNSSDYLHLNPMNFAIQTGEGGKYTVPGTVTLEDLQKFSDTFYCSCYANIHCKKRVDIKNVHSVNVCMAEDICIDSPVKLQPSDHSSSQEASTTTFSSISPSTTTATVSPCTPEKHSPECLKVRCSEVIPNVTQKACQSVKLKNISYQSPIQNIKNQTTY

Target ORF information:

RefSeq Version	XM_005205627.3
Organism	Bos taurus(cattle)
Definition	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205627.3

ORF Insert Sequence:

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
GGTGACCTTG TGAATTCGGT TGGTGAGATC GTTTCTCTAG GTGCCTCTGG GATTATAATG 
TGGGGCAGTC TCAATCTAAG CTTATCTATG CAATCTTGCA TGAACCTAGG CACTTACTTG 
AACACTACAC TGAATCCTTA CATAATCAAC GTCACCCTAG CCGCCAAAAT GTGCAGCCAA 
GTGCTTTGCC ACAATGAAGG AGTGTGTACA AGGAAACACT GGAATTCAAG CGACTATCTT 
CACCTGAACC CAATGAATTT TGCTATTCAA ACTGGGGAAG GTGGAAAATA CACAGTACCT 
GGGACAGTCA CACTTGAAGA CTTGCAAAAG TTTTCTGATA CATTTTATTG CAGTTGTTAT 
GCCAACATCC ACTGTAAGAA GAGAGTTGAT ATAAAAAATG TTCATAGTGT TAACGTGTGT 
ATGGCAGAAG ACATTTGTAT AGACAGCCCT GTGAAGTTAC AACCCAGTGA TCATTCCTCC 
AGCCAGGAGG CATCTACTAC CACCTTCAGC AGTATCTCAC CCTCCACTAC AACTGCCACA 
GTATCTCCAT GTACTCCTGA GAAACACTCC CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA 
GTCATCCCCA ACGTCACCCA AAAGGCGTGT CAAAGTGTTA AATTGAAGAA CATTTCCTAT 
CAGTCACCTA TTCAAAATAT TAAAAATCAA ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAM1 ( XM_024990501.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846269.1 Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa18710
Clone ID Related Accession (Same CDS sequence)	XM_005205627.4 , NM_001008413.3 , XM_024990500.1 , XM_005205627.3 , XM_024990501.1
Accession Version	XM_024990501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sperm adhesion molecule 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
GGTGACCTTG TGAATTCGGT TGGTGAGATC GTTTCTCTAG GTGCCTCTGG GATTATAATG 
TGGGGCAGTC TCAATCTAAG CTTATCTATG CAATCTTGCA TGAACCTAGG CACTTACTTG 
AACACTACAC TGAATCCTTA CATAATCAAC GTCACCCTAG CCGCCAAAAT GTGCAGCCAA 
GTGCTTTGCC ACAATGAAGG AGTGTGTACA AGGAAACACT GGAATTCAAG CGACTATCTT 
CACCTGAACC CAATGAATTT TGCTATTCAA ACTGGGGAAG GTGGAAAATA CACAGTACCT 
GGGACAGTCA CACTTGAAGA CTTGCAAAAG TTTTCTGATA CATTTTATTG CAGTTGTTAT 
GCCAACATCC ACTGTAAGAA GAGAGTTGAT ATAAAAAATG TTCATAGTGT TAACGTGTGT 
ATGGCAGAAG ACATTTGTAT AGACAGCCCT GTGAAGTTAC AACCCAGTGA TCATTCCTCC 
AGCCAGGAGG CATCTACTAC CACCTTCAGC AGTATCTCAC CCTCCACTAC AACTGCCACA 
GTATCTCCAT GTACTCCTGA GAAACACTCC CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA 
GTCATCCCCA ACGTCACCCA AAAGGCGTGT CAAAGTGTTA AATTGAAGAA CATTTCCTAT 
CAGTCACCTA TTCAAAATAT TAAAAATCAA ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846269.1
CDS	2629..4290
Translation	MRMLRRHHISFRSFAGSSGTPQAVFTFLLLPCCLALDFRAPPLISNTSFLWAWNAPVERCVNRRFQLPPDLRLFSVKGSPQKSATGQFITLFYADRLGYYPHIDEKTGKTVFGGIPQLGNLKSHLEKAKNDIAYYIPNDSVGLAVIDWENWRPTWARNWKPKDVYRDESVELVLQKNPQLSFPEASKIAKVDFETAGKSFMQETLKLGKLLRPNHLWGYYLFPDCYNHNHNQPTYNGNCPDVEKRRNDDLEWLWKESTALFPSVYLNIRLKSTQNAALYVRNRVQEAIRLSKIASVESPLPVFVYARPVFTDGSSTYLSQGDLVNSVGEIVSLGASGIIMWGSLNLSLSMQSCMNLGTYLNTTLNPYIINVTLAAKMCSQVLCHNEGVCTRKHWNSSDYLHLNPMNFAIQTGEGGKYTVPGTVTLEDLQKFSDTFYCSCYANIHCKKRVDIKNVHSVNVCMAEDICIDSPVKLQPSDHSSSQEASTTTFSSISPSTTTATVSPCTPEKHSPECLKVRCSEVIPNVTQKACQSVKLKNISYQSPIQNIKNQTTY

Target ORF information:

RefSeq Version	XM_024990501.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990501.1

ORF Insert Sequence:

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
GGTGACCTTG TGAATTCGGT TGGTGAGATC GTTTCTCTAG GTGCCTCTGG GATTATAATG 
TGGGGCAGTC TCAATCTAAG CTTATCTATG CAATCTTGCA TGAACCTAGG CACTTACTTG 
AACACTACAC TGAATCCTTA CATAATCAAC GTCACCCTAG CCGCCAAAAT GTGCAGCCAA 
GTGCTTTGCC ACAATGAAGG AGTGTGTACA AGGAAACACT GGAATTCAAG CGACTATCTT 
CACCTGAACC CAATGAATTT TGCTATTCAA ACTGGGGAAG GTGGAAAATA CACAGTACCT 
GGGACAGTCA CACTTGAAGA CTTGCAAAAG TTTTCTGATA CATTTTATTG CAGTTGTTAT 
GCCAACATCC ACTGTAAGAA GAGAGTTGAT ATAAAAAATG TTCATAGTGT TAACGTGTGT 
ATGGCAGAAG ACATTTGTAT AGACAGCCCT GTGAAGTTAC AACCCAGTGA TCATTCCTCC 
AGCCAGGAGG CATCTACTAC CACCTTCAGC AGTATCTCAC CCTCCACTAC AACTGCCACA 
GTATCTCCAT GTACTCCTGA GAAACACTCC CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA 
GTCATCCCCA ACGTCACCCA AAAGGCGTGT CAAAGTGTTA AATTGAAGAA CATTTCCTAT 
CAGTCACCTA TTCAAAATAT TAAAAATCAA ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAM1 ( XM_024990502.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846270.1 Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa18711
Clone ID Related Accession (Same CDS sequence)	XM_024990502.1 , XM_024990503.1
Accession Version	XM_024990502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sperm adhesion molecule 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
CAATCTTGCA TGAACCTAGG CACTTACTTG AACACTACAC TGAATCCTTA CATAATCAAC 
GTCACCCTAG CCGCCAAAAT GTGCAGCCAA GTGCTTTGCC ACAATGAAGG AGTGTGTACA 
AGGAAACACT GGAATTCAAG CGACTATCTT CACCTGAACC CAATGAATTT TGCTATTCAA 
ACTGGGGAAG GTGGAAAATA CACAGTACCT GGGACAGTCA CACTTGAAGA CTTGCAAAAG 
TTTTCTGATA CATTTTATTG CAGTTGTTAT GCCAACATCC ACTGTAAGAA GAGAGTTGAT 
ATAAAAAATG TTCATAGTGT TAACGTGTGT ATGGCAGAAG ACATTTGTAT AGACAGCCCT 
GTGAAGTTAC AACCCAGTGA TCATTCCTCC AGCCAGGAGG CATCTACTAC CACCTTCAGC 
AGTATCTCAC CCTCCACTAC AACTGCCACA GTATCTCCAT GTACTCCTGA GAAACACTCC 
CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA GTCATCCCCA ACGTCACCCA AAAGGCGTGT 
CAAAGTGTTA AATTGAAGAA CATTTCCTAT CAGTCACCTA TTCAAAATAT TAAAAATCAA 
ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846270.1
CDS	2597..4168
Translation	MRMLRRHHISFRSFAGSSGTPQAVFTFLLLPCCLALDFRAPPLISNTSFLWAWNAPVERCVNRRFQLPPDLRLFSVKGSPQKSATGQFITLFYADRLGYYPHIDEKTGKTVFGGIPQLGNLKSHLEKAKNDIAYYIPNDSVGLAVIDWENWRPTWARNWKPKDVYRDESVELVLQKNPQLSFPEASKIAKVDFETAGKSFMQETLKLGKLLRPNHLWGYYLFPDCYNHNHNQPTYNGNCPDVEKRRNDDLEWLWKESTALFPSVYLNIRLKSTQNAALYVRNRVQEAIRLSKIASVESPLPVFVYARPVFTDGSSTYLSQQSCMNLGTYLNTTLNPYIINVTLAAKMCSQVLCHNEGVCTRKHWNSSDYLHLNPMNFAIQTGEGGKYTVPGTVTLEDLQKFSDTFYCSCYANIHCKKRVDIKNVHSVNVCMAEDICIDSPVKLQPSDHSSSQEASTTTFSSISPSTTTATVSPCTPEKHSPECLKVRCSEVIPNVTQKACQSVKLKNISYQSPIQNIKNQTTY

Target ORF information:

RefSeq Version	XM_024990502.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990502.1

ORF Insert Sequence:

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
CAATCTTGCA TGAACCTAGG CACTTACTTG AACACTACAC TGAATCCTTA CATAATCAAC 
GTCACCCTAG CCGCCAAAAT GTGCAGCCAA GTGCTTTGCC ACAATGAAGG AGTGTGTACA 
AGGAAACACT GGAATTCAAG CGACTATCTT CACCTGAACC CAATGAATTT TGCTATTCAA 
ACTGGGGAAG GTGGAAAATA CACAGTACCT GGGACAGTCA CACTTGAAGA CTTGCAAAAG 
TTTTCTGATA CATTTTATTG CAGTTGTTAT GCCAACATCC ACTGTAAGAA GAGAGTTGAT 
ATAAAAAATG TTCATAGTGT TAACGTGTGT ATGGCAGAAG ACATTTGTAT AGACAGCCCT 
GTGAAGTTAC AACCCAGTGA TCATTCCTCC AGCCAGGAGG CATCTACTAC CACCTTCAGC 
AGTATCTCAC CCTCCACTAC AACTGCCACA GTATCTCCAT GTACTCCTGA GAAACACTCC 
CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA GTCATCCCCA ACGTCACCCA AAAGGCGTGT 
CAAAGTGTTA AATTGAAGAA CATTTCCTAT CAGTCACCTA TTCAAAATAT TAAAAATCAA 
ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAM1 ( XM_024990503.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846271.1 Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X5, mRNA.

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CloneID	OBa18711
Clone ID Related Accession (Same CDS sequence)	XM_024990502.1 , XM_024990503.1
Accession Version	XM_024990503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sperm adhesion molecule 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
CAATCTTGCA TGAACCTAGG CACTTACTTG AACACTACAC TGAATCCTTA CATAATCAAC 
GTCACCCTAG CCGCCAAAAT GTGCAGCCAA GTGCTTTGCC ACAATGAAGG AGTGTGTACA 
AGGAAACACT GGAATTCAAG CGACTATCTT CACCTGAACC CAATGAATTT TGCTATTCAA 
ACTGGGGAAG GTGGAAAATA CACAGTACCT GGGACAGTCA CACTTGAAGA CTTGCAAAAG 
TTTTCTGATA CATTTTATTG CAGTTGTTAT GCCAACATCC ACTGTAAGAA GAGAGTTGAT 
ATAAAAAATG TTCATAGTGT TAACGTGTGT ATGGCAGAAG ACATTTGTAT AGACAGCCCT 
GTGAAGTTAC AACCCAGTGA TCATTCCTCC AGCCAGGAGG CATCTACTAC CACCTTCAGC 
AGTATCTCAC CCTCCACTAC AACTGCCACA GTATCTCCAT GTACTCCTGA GAAACACTCC 
CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA GTCATCCCCA ACGTCACCCA AAAGGCGTGT 
CAAAGTGTTA AATTGAAGAA CATTTCCTAT CAGTCACCTA TTCAAAATAT TAAAAATCAA 
ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846271.1
CDS	2586..4157
Translation	MRMLRRHHISFRSFAGSSGTPQAVFTFLLLPCCLALDFRAPPLISNTSFLWAWNAPVERCVNRRFQLPPDLRLFSVKGSPQKSATGQFITLFYADRLGYYPHIDEKTGKTVFGGIPQLGNLKSHLEKAKNDIAYYIPNDSVGLAVIDWENWRPTWARNWKPKDVYRDESVELVLQKNPQLSFPEASKIAKVDFETAGKSFMQETLKLGKLLRPNHLWGYYLFPDCYNHNHNQPTYNGNCPDVEKRRNDDLEWLWKESTALFPSVYLNIRLKSTQNAALYVRNRVQEAIRLSKIASVESPLPVFVYARPVFTDGSSTYLSQQSCMNLGTYLNTTLNPYIINVTLAAKMCSQVLCHNEGVCTRKHWNSSDYLHLNPMNFAIQTGEGGKYTVPGTVTLEDLQKFSDTFYCSCYANIHCKKRVDIKNVHSVNVCMAEDICIDSPVKLQPSDHSSSQEASTTTFSSISPSTTTATVSPCTPEKHSPECLKVRCSEVIPNVTQKACQSVKLKNISYQSPIQNIKNQTTY

Target ORF information:

RefSeq Version	XM_024990503.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990503.1

ORF Insert Sequence:

ATGAGAATGC TGAGGCGCCA CCATATCTCC TTTCGGAGCT TTGCTGGGTC TAGCGGAACA 
CCCCAGGCAG TGTTCACCTT CCTTCTGCTT CCGTGTTGTT TGGCTCTGGA CTTCAGAGCA 
CCCCCTCTTA TTTCAAACAC TTCTTTCCTC TGGGCCTGGA ATGCCCCAGT TGAACGTTGT 
GTTAACAGAA GATTTCAACT ACCTCCAGAT CTGAGACTCT TCTCTGTAAA AGGAAGCCCC 
CAGAAAAGTG CTACCGGACA ATTTATTACA TTATTTTATG CTGATAGACT TGGCTACTAT 
CCTCATATAG ATGAAAAAAC AGGCAAAACC GTATTCGGAG GAATTCCCCA GTTGGGAAAC 
TTAAAAAGTC ATTTGGAGAA AGCAAAAAAT GACATTGCCT ATTACATACC AAATGACAGC 
GTGGGCTTGG CGGTCATTGA CTGGGAAAAC TGGAGGCCTA CCTGGGCAAG AAACTGGAAA 
CCTAAAGATG TTTACAGGGA TGAGTCAGTT GAGTTGGTTC TGCAAAAAAA TCCGCAACTC 
AGTTTCCCAG AGGCTTCCAA GATTGCAAAA GTGGATTTTG AGACAGCAGG AAAGAGTTTC 
ATGCAAGAGA CTTTAAAACT GGGAAAATTA CTTCGGCCAA ATCACTTATG GGGTTATTAT 
CTTTTTCCTG ATTGTTACAA TCATAATCAT AACCAACCTA CTTACAATGG AAATTGCCCT 
GATGTAGAAA AAAGGAGAAA TGATGATCTC GAGTGGTTGT GGAAGGAAAG CACTGCCCTT 
TTCCCTTCTG TTTATTTGAA TATCAGGTTA AAATCTACTC AAAATGCTGC CTTGTATGTT 
CGTAATCGTG TCCAGGAAGC CATTCGGTTG TCTAAAATAG CGAGTGTCGA AAGTCCACTT 
CCGGTTTTTG TATATGCCCG TCCAGTTTTT ACTGATGGGT CTTCAACATA TCTTTCTCAG 
CAATCTTGCA TGAACCTAGG CACTTACTTG AACACTACAC TGAATCCTTA CATAATCAAC 
GTCACCCTAG CCGCCAAAAT GTGCAGCCAA GTGCTTTGCC ACAATGAAGG AGTGTGTACA 
AGGAAACACT GGAATTCAAG CGACTATCTT CACCTGAACC CAATGAATTT TGCTATTCAA 
ACTGGGGAAG GTGGAAAATA CACAGTACCT GGGACAGTCA CACTTGAAGA CTTGCAAAAG 
TTTTCTGATA CATTTTATTG CAGTTGTTAT GCCAACATCC ACTGTAAGAA GAGAGTTGAT 
ATAAAAAATG TTCATAGTGT TAACGTGTGT ATGGCAGAAG ACATTTGTAT AGACAGCCCT 
GTGAAGTTAC AACCCAGTGA TCATTCCTCC AGCCAGGAGG CATCTACTAC CACCTTCAGC 
AGTATCTCAC CCTCCACTAC AACTGCCACA GTATCTCCAT GTACTCCTGA GAAACACTCC 
CCTGAGTGCC TCAAAGTCAG GTGTTCGGAA GTCATCCCCA ACGTCACCCA AAAGGCGTGT 
CAAAGTGTTA AATTGAAGAA CATTTCCTAT CAGTCACCTA TTCAAAATAT TAAAAATCAA 
ACAACCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stratification of chondroitin sulfate binding sites in 3D-model of bovine testicular hyaluronidase and effective size of glycosaminoglycan coat of the modified protein.
Biochemistry. Biokhimiia80(3)284-95(2015 Mar)
Maksimenko AV,Turashev AD,Beabealashvili RS

Effects of divalent cations on bovine testicular hyaluronidase catalyzed transglycosylation of chondroitin sulfates.
Biochemical and biophysical research communications406(2)239-44(2011 Mar)
Kakizaki I,Nukatsuka I,Takagaki K,Majima M,Iwafune M,Suto S,Endo M

SPAM1 isoforms from two tissue origins are differentially localized within ejaculated bull sperm membranes and have different roles during fertilization.
Biology of reproduction82(2)271-81(2010 Feb)
Morin G,Sullivan R,Laflamme I,Robert C,Leclerc P

Development of a fluorescent substrate to measure hyaluronidase activity.
Analytical biochemistry379(1)80-5(2008 Aug)
Zhang LS,Mummert ME

Chain-length dependence of the kinetics of the hyaluronan hydrolysis catalyzed by bovine testicular hyaluronidase.
Matrix biology : journal of the International Society for Matrix Biology27(5)475-86(2008 Jun)
Deschrevel B,Tranchepain F,Vincent JC

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SPAM1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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