SPAG4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAG4
Entrez Gene ID	6676
Full Name	sperm associated antigen 4
Synonyms	CT127,SUN4
General protein information	Preferred Names sperm associated antigen 4 Names sperm-associated antigen 4 protein SUN domain-containing protein 4 Sad1 and UNC84 domain containing 4 acrosomal protein ACR55 cancer/testis antigen 127 outer dense fiber-associated protein SPAG4 sperm tail protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q11.22
Summary	The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015].
Disorder MIM:	603038

mRNA and Protein(s)

mRNA	Protein	Name
NM_001317931.1	NP_001304860.1	sperm-associated antigen 4 protein isoform 2
NM_003116.1	NP_003107.1	sperm-associated antigen 4 protein
XM_005260520.4	XP_005260577.1	sperm-associated antigen 4 protein isoform X2
XM_011529009.2	XP_011527311.1	sperm-associated antigen 4 protein isoform X1
NM_003116.3	NP_003107.1	sperm-associated antigen 4 protein isoform 1
NM_003116.2	NP_003107.1	sperm-associated antigen 4 protein isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	LOC100911109	XP_003749659.1
Mus musculus (house mouse)	Spag4	NP_631890.3
Homo sapiens (human)	SPAG4	NP_003107.1
Pan troglodytes (chimpanzee)	SPAG4	XP_514609.2
	SPAG4	XP_001098382.1
Canis lupus familiaris (dog)	SPAG4	XP_542978.2
Bos taurus (cattle)	SPAG4	NP_001069975.1
Rattus norvegicus (Norway rat)	Spag4	NP_113980.1

Gene Ontology
Bibliography

Related articles in PubMed

SEPT12/SPAG4/LAMINB1 complexes are required for maintaining the integrity of the nuclear envelope in postmeiotic male germ cells.
Yeh CH, Kuo PL, Wang YY, Wu YY, Chen MF, Lin DY, Lai TH, Chiang HS, Lin YH
PloS one10(3)e0120722(2015)

Hypoxia regulates the sperm associated antigen 4 (SPAG4) via HIF, which is expressed in renal clear cell carcinoma and promotes migration and invasion in vitro.
Knaup KX, Monti J, Hackenbeck T, Jobst-Schwan T, Klanke B, Schietke RE, Wacker I, Behrens J, Amann K, Eckardt KU, Warnecke C, Wiesener MS
Molecular carcinogenesis53(12)970-8(2014 Dec)

Sperm-associated antigen 4, a novel hypoxia-inducible factor 1 target, regulates cytokinesis, and its expression correlates with the prognosis of renal cell carcinoma.
Shoji K, Murayama T, Mimura I, Wada T, Kume H, Goto A, Ohse T, Tanaka T, Inagi R, van der Hoorn FA, Manabe I, Homma Y, Fukayama M, Sakurai T, Hasegawa T, Aburatani H, Kodama T, Nangaku M
The American journal of pathology182(6)2191-203(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPAG4 knockdown reduces the invasion capability of RCC cells.
Title: Hypoxia regulates the sperm associated antigen 4 (SPAG4) via HIF, which is expressed in renal clear cell carcinoma and promotes migration and invasion in vitro.

SPAG4 is an independent prognostic factor in renal cell carcinoma and plays a crucial role in cytokinesis to defend against hypoxia-induced tetraploid formation.
Title: Sperm-associated antigen 4, a novel hypoxia-inducible factor 1 target, regulates cytokinesis, and its expression correlates with the prognosis of renal cell carcinoma.

The following SPAG4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAG4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03239	NM_003116.3 Latest version!	Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu53096	NM_001317931.1 Latest version!	Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu53097	XM_005260520.4 Latest version!	Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu60701	XM_011529009.2 Latest version!	Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu03239	NM_003116.1	Homo sapiens sperm associated antigen 4 (SPAG4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu03239	NM_003116.2	Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAG4 ( NM_003116.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_003107.1 Homo sapiens sperm associated antigen 4 (SPAG4), mRNA.

Price & Availability↑

CloneID	OHu03239
Clone ID Related Accession (Same CDS sequence)	NM_003116.1 , NM_003116.3 , NM_003116.2
Accession Version	NM_003116.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	sperm-associated antigen 4 protein
Comment

ATGCGGCGAA GCTCCCGCCC GGGCTCGGCC TCGTCCTCGC GCAAGCACAC GCCCAACTTT 
TTCAGCGAGA ACAGCTCAAT GAGCATCACC TCGGAGGACA GCAAAGGGCT CCGGTCAGCG 
GAGCCCGGGC CTGGGGAGCC CGAGGGCAGA AGAGCCCGGG GCCCGAGCTG CGGTGAGCCC 
GCCTTGAGCG CGGGAGTGCC CGGAGGAACC ACATGGGCAG GAAGCTCTCA GCAGAAGCCA 
GCGCCTCGGA GCCACAACTG GCAGACAGCC TGTGGCGCGG CAACCGTGAG GGGCGGGGCC 
TCGGAACCGA CTGGGTCTCC AGTAGTCTCT GAGGAGCCGC TCGACCTTCT CCCGACCCTG 
GATCTGAGGC AGGAGATGCC TCCCCCGCGG GTGTTCAAGA GCTTTCTGAG CCTGCTCTTC 
CAGGGGCTGA GCGTGTTGTT ATCCCTGGCA GGAGACGTGC TGGTCAGCAT GTACAGGGAG 
GTCTGTTCCA TCCGCTTCCT GTTCACGGCT GTGTCGCTGC TGAGCCTCTT TCTGTCAGCA 
TTCTGGCTGG GGCTTCTGTA CCTGGTCTCT CCTTTGGAGA ATGAACCTAA GGAGATGCTG 
ACTCTAAGTG AGTACCACGA GCGCGTGCGC TCCCAGGGGC AGCAGCTGCA GCAGCTCCAG 
GCCGAGCTGG ATAAACTCCA CAAGGAGGTG TCCACTGTTC GGGCAGCCAA CAGCGAGAGA 
GTGGCCAAGC TCGTGTTCCA GAGGCTGAAT GAGGATTTTG TGCGGAAGCC CGACTATGCT 
TTGAGCTCTG TGGGAGCCTC CATCGACCTG CAGAAGACAT CCCACGATTA CGCAGACAGG 
AACACTGCCT ACTTCTGGAA TCGCTTCAGC TTCTGGAACT ACGCACGGCC GCCCACGGTT 
ATCCTGGAGC CCCACGTGTT CCCTGGGAAT TGCTGGGCTT TTGAAGGCGA CCAAGGCCAG 
GTGGTGATCC AACTGCCGGG CCGAGTGCAG CTGAGCGACA TCACTCTGCA GCATCCACCG 
CCCAGCGTGG AGCACACCGG AGGAGCCAAC AGCGCCCCCC GCGATTTCGC GGTCTTTGGC 
CTCCAGGTTT ATGATGAAAC TGAAGTTTCC TTGGGGAAAT TCACCTTCGA TGTTGAGAAA 
TCGGAGATTC AGACTTTCCA CCTGCAGAAT GACCCCCCAG CTGCCTTTCC CAAGGTGAAG 
ATCCAGATTC TAAGCAACTG GGGCCACCCC CGTTTCACGT GCTTGTATCG AGTCCGTGCC 
CACGGTGTGC GAACCTCAGA GGGGGCAGAG GGCAGTGCAC AGGGGCCCCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_003107.1

Translation

MRRSSRPGSASSSRKHTPNFFSENSSMSITSEDSKGLRSAEPGPGEPEGRRARGPSCGEPALSAGVPGGTTWAGSSQQKPAPRSHNWQTACGAATVRGGASEPTGSPVVSEEPLDLLPTLDLRQEMPPPRVFKSFLSLLFQGLSVLLSLAGDVLVSMYREVCSIRFLFTAVSLLSLFLSAFWLGLLYLVSPLENEPKEMLTLSEYHERVRSQGQQLQQLQAELDKLHKEVSTVRAANSERVAKLVFQRLNEDFVRKPDYALSSVGASIDLQKTSHDYADRNTAYFWNRFSFWNYARPPTVILEPHVFPGNCWAFEGDQGQVVIQLPGRVQLSDITLQHPPPSVEHTGGANSAPRDFAVFGLQVYDETEVSLGKFTFDVEKSEIQTFHLQNDPPAAFPKVKIQILSNWGHPRFTCLYRVRAHGVRTSEGAEGSAQGPH

Target ORF information:

RefSeq Version	NM_003116.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens sperm associated antigen 4 (SPAG4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003116.1

ORF Insert Sequence:

ATGCGGCGAA GCTCCCGCCC GGGCTCGGCC TCGTCCTCGC GCAAGCACAC GCCCAACTTT 
TTCAGCGAGA ACAGCTCAAT GAGCATCACC TCGGAGGACA GCAAAGGGCT CCGGTCAGCG 
GAGCCCGGGC CTGGGGAGCC CGAGGGCAGA AGAGCCCGGG GCCCGAGCTG CGGTGAGCCC 
GCCTTGAGCG CGGGAGTGCC CGGAGGAACC ACATGGGCAG GAAGCTCTCA GCAGAAGCCA 
GCGCCTCGGA GCCACAACTG GCAGACAGCC TGTGGCGCGG CAACCGTGAG GGGCGGGGCC 
TCGGAACCGA CTGGGTCTCC AGTAGTCTCT GAGGAGCCGC TCGACCTTCT CCCGACCCTG 
GATCTGAGGC AGGAGATGCC TCCCCCGCGG GTGTTCAAGA GCTTTCTGAG CCTGCTCTTC 
CAGGGGCTGA GCGTGTTGTT ATCCCTGGCA GGAGACGTGC TGGTCAGCAT GTACAGGGAG 
GTCTGTTCCA TCCGCTTCCT GTTCACGGCT GTGTCGCTGC TGAGCCTCTT TCTGTCAGCA 
TTCTGGCTGG GGCTTCTGTA CCTGGTCTCT CCTTTGGAGA ATGAACCTAA GGAGATGCTG 
ACTCTAAGTG AGTACCACGA GCGCGTGCGC TCCCAGGGGC AGCAGCTGCA GCAGCTCCAG 
GCCGAGCTGG ATAAACTCCA CAAGGAGGTG TCCACTGTTC GGGCAGCCAA CAGCGAGAGA 
GTGGCCAAGC TCGTGTTCCA GAGGCTGAAT GAGGATTTTG TGCGGAAGCC CGACTATGCT 
TTGAGCTCTG TGGGAGCCTC CATCGACCTG CAGAAGACAT CCCACGATTA CGCAGACAGG 
AACACTGCCT ACTTCTGGAA TCGCTTCAGC TTCTGGAACT ACGCACGGCC GCCCACGGTT 
ATCCTGGAGC CCCACGTGTT CCCTGGGAAT TGCTGGGCTT TTGAAGGCGA CCAAGGCCAG 
GTGGTGATCC AACTGCCGGG CCGAGTGCAG CTGAGCGACA TCACTCTGCA GCATCCACCG 
CCCAGCGTGG AGCACACCGG AGGAGCCAAC AGCGCCCCCC GCGATTTCGC GGTCTTTGGC 
CTCCAGGTTT ATGATGAAAC TGAAGTTTCC TTGGGGAAAT TCACCTTCGA TGTTGAGAAA 
TCGGAGATTC AGACTTTCCA CCTGCAGAAT GACCCCCCAG CTGCCTTTCC CAAGGTGAAG 
ATCCAGATTC TAAGCAACTG GGGCCACCCC CGTTTCACGT GCTTGTATCG AGTCCGTGCC 
CACGGTGTGC GAACCTCAGA GGGGGCAGAG GGCAGTGCAC AGGGGCCCCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG4 ( NM_003116.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003107.1 Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03239
Clone ID Related Accession (Same CDS sequence)	NM_003116.1 , NM_003116.3 , NM_003116.2
Accession Version	NM_003116.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	sperm-associated antigen 4 protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL109827.8, HY021678.1, AF262992.1 and AI168233.1. On May 17, 2019 this sequence version replaced NM_003116.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AF262992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374273.8/ ENSP00000363391.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGCGAA GCTCCCGCCC GGGCTCGGCC TCGTCCTCGC GCAAGCACAC GCCCAACTTT 
TTCAGCGAGA ACAGCTCAAT GAGCATCACC TCGGAGGACA GCAAAGGGCT CCGGTCAGCG 
GAGCCCGGGC CTGGGGAGCC CGAGGGCAGA AGAGCCCGGG GCCCGAGCTG CGGTGAGCCC 
GCCTTGAGCG CGGGAGTGCC CGGAGGAACC ACATGGGCAG GAAGCTCTCA GCAGAAGCCA 
GCGCCTCGGA GCCACAACTG GCAGACAGCC TGTGGCGCGG CAACCGTGAG GGGCGGGGCC 
TCGGAACCGA CTGGGTCTCC AGTAGTCTCT GAGGAGCCGC TCGACCTTCT CCCGACCCTG 
GATCTGAGGC AGGAGATGCC TCCCCCGCGG GTGTTCAAGA GCTTTCTGAG CCTGCTCTTC 
CAGGGGCTGA GCGTGTTGTT ATCCCTGGCA GGAGACGTGC TGGTCAGCAT GTACAGGGAG 
GTCTGTTCCA TCCGCTTCCT GTTCACGGCT GTGTCGCTGC TGAGCCTCTT TCTGTCAGCA 
TTCTGGCTGG GGCTTCTGTA CCTGGTCTCT CCTTTGGAGA ATGAACCTAA GGAGATGCTG 
ACTCTAAGTG AGTACCACGA GCGCGTGCGC TCCCAGGGGC AGCAGCTGCA GCAGCTCCAG 
GCCGAGCTGG ATAAACTCCA CAAGGAGGTG TCCACTGTTC GGGCAGCCAA CAGCGAGAGA 
GTGGCCAAGC TCGTGTTCCA GAGGCTGAAT GAGGATTTTG TGCGGAAGCC CGACTATGCT 
TTGAGCTCTG TGGGAGCCTC CATCGACCTG CAGAAGACAT CCCACGATTA CGCAGACAGG 
AACACTGCCT ACTTCTGGAA TCGCTTCAGC TTCTGGAACT ACGCACGGCC GCCCACGGTT 
ATCCTGGAGC CCCACGTGTT CCCTGGGAAT TGCTGGGCTT TTGAAGGCGA CCAAGGCCAG 
GTGGTGATCC AACTGCCGGG CCGAGTGCAG CTGAGCGACA TCACTCTGCA GCATCCACCG 
CCCAGCGTGG AGCACACCGG AGGAGCCAAC AGCGCCCCCC GCGATTTCGC GGTCTTTGGC 
CTCCAGGTTT ATGATGAAAC TGAAGTTTCC TTGGGGAAAT TCACCTTCGA TGTTGAGAAA 
TCGGAGATTC AGACTTTCCA CCTGCAGAAT GACCCCCCAG CTGCCTTTCC CAAGGTGAAG 
ATCCAGATTC TAAGCAACTG GGGCCACCCC CGTTTCACGT GCTTGTATCG AGTCCGTGCC 
CACGGTGTGC GAACCTCAGA GGGGGCAGAG GGCAGTGCAC AGGGGCCCCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003107.1
CDS	176..1489
Translation	MRRSSRPGSASSSRKHTPNFFSENSSMSITSEDSKGLRSAEPGPGEPEGRRARGPSCGEPALSAGVPGGTTWAGSSQQKPAPRSHNWQTACGAATVRGGASEPTGSPVVSEEPLDLLPTLDLRQEMPPPRVFKSFLSLLFQGLSVLLSLAGDVLVSMYREVCSIRFLFTAVSLLSLFLSAFWLGLLYLVSPLENEPKEMLTLSEYHERVRSQGQQLQQLQAELDKLHKEVSTVRAANSERVAKLVFQRLNEDFVRKPDYALSSVGASIDLQKTSHDYADRNTAYFWNRFSFWNYARPPTVILEPHVFPGNCWAFEGDQGQVVIQLPGRVQLSDITLQHPPPSVEHTGGANSAPRDFAVFGLQVYDETEVSLGKFTFDVEKSEIQTFHLQNDPPAAFPKVKIQILSNWGHPRFTCLYRVRAHGVRTSEGAEGSAQGPH

Target ORF information:

RefSeq Version	NM_003116.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003116.3

ORF Insert Sequence:

ATGCGGCGAA GCTCCCGCCC GGGCTCGGCC TCGTCCTCGC GCAAGCACAC GCCCAACTTT 
TTCAGCGAGA ACAGCTCAAT GAGCATCACC TCGGAGGACA GCAAAGGGCT CCGGTCAGCG 
GAGCCCGGGC CTGGGGAGCC CGAGGGCAGA AGAGCCCGGG GCCCGAGCTG CGGTGAGCCC 
GCCTTGAGCG CGGGAGTGCC CGGAGGAACC ACATGGGCAG GAAGCTCTCA GCAGAAGCCA 
GCGCCTCGGA GCCACAACTG GCAGACAGCC TGTGGCGCGG CAACCGTGAG GGGCGGGGCC 
TCGGAACCGA CTGGGTCTCC AGTAGTCTCT GAGGAGCCGC TCGACCTTCT CCCGACCCTG 
GATCTGAGGC AGGAGATGCC TCCCCCGCGG GTGTTCAAGA GCTTTCTGAG CCTGCTCTTC 
CAGGGGCTGA GCGTGTTGTT ATCCCTGGCA GGAGACGTGC TGGTCAGCAT GTACAGGGAG 
GTCTGTTCCA TCCGCTTCCT GTTCACGGCT GTGTCGCTGC TGAGCCTCTT TCTGTCAGCA 
TTCTGGCTGG GGCTTCTGTA CCTGGTCTCT CCTTTGGAGA ATGAACCTAA GGAGATGCTG 
ACTCTAAGTG AGTACCACGA GCGCGTGCGC TCCCAGGGGC AGCAGCTGCA GCAGCTCCAG 
GCCGAGCTGG ATAAACTCCA CAAGGAGGTG TCCACTGTTC GGGCAGCCAA CAGCGAGAGA 
GTGGCCAAGC TCGTGTTCCA GAGGCTGAAT GAGGATTTTG TGCGGAAGCC CGACTATGCT 
TTGAGCTCTG TGGGAGCCTC CATCGACCTG CAGAAGACAT CCCACGATTA CGCAGACAGG 
AACACTGCCT ACTTCTGGAA TCGCTTCAGC TTCTGGAACT ACGCACGGCC GCCCACGGTT 
ATCCTGGAGC CCCACGTGTT CCCTGGGAAT TGCTGGGCTT TTGAAGGCGA CCAAGGCCAG 
GTGGTGATCC AACTGCCGGG CCGAGTGCAG CTGAGCGACA TCACTCTGCA GCATCCACCG 
CCCAGCGTGG AGCACACCGG AGGAGCCAAC AGCGCCCCCC GCGATTTCGC GGTCTTTGGC 
CTCCAGGTTT ATGATGAAAC TGAAGTTTCC TTGGGGAAAT TCACCTTCGA TGTTGAGAAA 
TCGGAGATTC AGACTTTCCA CCTGCAGAAT GACCCCCCAG CTGCCTTTCC CAAGGTGAAG 
ATCCAGATTC TAAGCAACTG GGGCCACCCC CGTTTCACGT GCTTGTATCG AGTCCGTGCC 
CACGGTGTGC GAACCTCAGA GGGGGCAGAG GGCAGTGCAC AGGGGCCCCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG4 ( NM_003116.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003107.1 Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03239
Clone ID Related Accession (Same CDS sequence)	NM_003116.1 , NM_003116.3 , NM_003116.2
Accession Version	NM_003116.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-20
Organism	Homo sapiens(human)
Product	sperm-associated antigen 4 protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY021678.1, AF262992.1 and AI168233.1. On Dec 15, 2015 this sequence version replaced NM_003116.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AF262992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCGAA GCTCCCGCCC GGGCTCGGCC TCGTCCTCGC GCAAGCACAC GCCCAACTTT 
TTCAGCGAGA ACAGCTCAAT GAGCATCACC TCGGAGGACA GCAAAGGGCT CCGGTCAGCG 
GAGCCCGGGC CTGGGGAGCC CGAGGGCAGA AGAGCCCGGG GCCCGAGCTG CGGTGAGCCC 
GCCTTGAGCG CGGGAGTGCC CGGAGGAACC ACATGGGCAG GAAGCTCTCA GCAGAAGCCA 
GCGCCTCGGA GCCACAACTG GCAGACAGCC TGTGGCGCGG CAACCGTGAG GGGCGGGGCC 
TCGGAACCGA CTGGGTCTCC AGTAGTCTCT GAGGAGCCGC TCGACCTTCT CCCGACCCTG 
GATCTGAGGC AGGAGATGCC TCCCCCGCGG GTGTTCAAGA GCTTTCTGAG CCTGCTCTTC 
CAGGGGCTGA GCGTGTTGTT ATCCCTGGCA GGAGACGTGC TGGTCAGCAT GTACAGGGAG 
GTCTGTTCCA TCCGCTTCCT GTTCACGGCT GTGTCGCTGC TGAGCCTCTT TCTGTCAGCA 
TTCTGGCTGG GGCTTCTGTA CCTGGTCTCT CCTTTGGAGA ATGAACCTAA GGAGATGCTG 
ACTCTAAGTG AGTACCACGA GCGCGTGCGC TCCCAGGGGC AGCAGCTGCA GCAGCTCCAG 
GCCGAGCTGG ATAAACTCCA CAAGGAGGTG TCCACTGTTC GGGCAGCCAA CAGCGAGAGA 
GTGGCCAAGC TCGTGTTCCA GAGGCTGAAT GAGGATTTTG TGCGGAAGCC CGACTATGCT 
TTGAGCTCTG TGGGAGCCTC CATCGACCTG CAGAAGACAT CCCACGATTA CGCAGACAGG 
AACACTGCCT ACTTCTGGAA TCGCTTCAGC TTCTGGAACT ACGCACGGCC GCCCACGGTT 
ATCCTGGAGC CCCACGTGTT CCCTGGGAAT TGCTGGGCTT TTGAAGGCGA CCAAGGCCAG 
GTGGTGATCC AACTGCCGGG CCGAGTGCAG CTGAGCGACA TCACTCTGCA GCATCCACCG 
CCCAGCGTGG AGCACACCGG AGGAGCCAAC AGCGCCCCCC GCGATTTCGC GGTCTTTGGC 
CTCCAGGTTT ATGATGAAAC TGAAGTTTCC TTGGGGAAAT TCACCTTCGA TGTTGAGAAA 
TCGGAGATTC AGACTTTCCA CCTGCAGAAT GACCCCCCAG CTGCCTTTCC CAAGGTGAAG 
ATCCAGATTC TAAGCAACTG GGGCCACCCC CGTTTCACGT GCTTGTATCG AGTCCGTGCC 
CACGGTGTGC GAACCTCAGA GGGGGCAGAG GGCAGTGCAC AGGGGCCCCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003107.1
CDS	121..1434
Translation	MRRSSRPGSASSSRKHTPNFFSENSSMSITSEDSKGLRSAEPGPGEPEGRRARGPSCGEPALSAGVPGGTTWAGSSQQKPAPRSHNWQTACGAATVRGGASEPTGSPVVSEEPLDLLPTLDLRQEMPPPRVFKSFLSLLFQGLSVLLSLAGDVLVSMYREVCSIRFLFTAVSLLSLFLSAFWLGLLYLVSPLENEPKEMLTLSEYHERVRSQGQQLQQLQAELDKLHKEVSTVRAANSERVAKLVFQRLNEDFVRKPDYALSSVGASIDLQKTSHDYADRNTAYFWNRFSFWNYARPPTVILEPHVFPGNCWAFEGDQGQVVIQLPGRVQLSDITLQHPPPSVEHTGGANSAPRDFAVFGLQVYDETEVSLGKFTFDVEKSEIQTFHLQNDPPAAFPKVKIQILSNWGHPRFTCLYRVRAHGVRTSEGAEGSAQGPH

Target ORF information:

RefSeq Version	NM_003116.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003116.2

ORF Insert Sequence:

ATGCGGCGAA GCTCCCGCCC GGGCTCGGCC TCGTCCTCGC GCAAGCACAC GCCCAACTTT 
TTCAGCGAGA ACAGCTCAAT GAGCATCACC TCGGAGGACA GCAAAGGGCT CCGGTCAGCG 
GAGCCCGGGC CTGGGGAGCC CGAGGGCAGA AGAGCCCGGG GCCCGAGCTG CGGTGAGCCC 
GCCTTGAGCG CGGGAGTGCC CGGAGGAACC ACATGGGCAG GAAGCTCTCA GCAGAAGCCA 
GCGCCTCGGA GCCACAACTG GCAGACAGCC TGTGGCGCGG CAACCGTGAG GGGCGGGGCC 
TCGGAACCGA CTGGGTCTCC AGTAGTCTCT GAGGAGCCGC TCGACCTTCT CCCGACCCTG 
GATCTGAGGC AGGAGATGCC TCCCCCGCGG GTGTTCAAGA GCTTTCTGAG CCTGCTCTTC 
CAGGGGCTGA GCGTGTTGTT ATCCCTGGCA GGAGACGTGC TGGTCAGCAT GTACAGGGAG 
GTCTGTTCCA TCCGCTTCCT GTTCACGGCT GTGTCGCTGC TGAGCCTCTT TCTGTCAGCA 
TTCTGGCTGG GGCTTCTGTA CCTGGTCTCT CCTTTGGAGA ATGAACCTAA GGAGATGCTG 
ACTCTAAGTG AGTACCACGA GCGCGTGCGC TCCCAGGGGC AGCAGCTGCA GCAGCTCCAG 
GCCGAGCTGG ATAAACTCCA CAAGGAGGTG TCCACTGTTC GGGCAGCCAA CAGCGAGAGA 
GTGGCCAAGC TCGTGTTCCA GAGGCTGAAT GAGGATTTTG TGCGGAAGCC CGACTATGCT 
TTGAGCTCTG TGGGAGCCTC CATCGACCTG CAGAAGACAT CCCACGATTA CGCAGACAGG 
AACACTGCCT ACTTCTGGAA TCGCTTCAGC TTCTGGAACT ACGCACGGCC GCCCACGGTT 
ATCCTGGAGC CCCACGTGTT CCCTGGGAAT TGCTGGGCTT TTGAAGGCGA CCAAGGCCAG 
GTGGTGATCC AACTGCCGGG CCGAGTGCAG CTGAGCGACA TCACTCTGCA GCATCCACCG 
CCCAGCGTGG AGCACACCGG AGGAGCCAAC AGCGCCCCCC GCGATTTCGC GGTCTTTGGC 
CTCCAGGTTT ATGATGAAAC TGAAGTTTCC TTGGGGAAAT TCACCTTCGA TGTTGAGAAA 
TCGGAGATTC AGACTTTCCA CCTGCAGAAT GACCCCCCAG CTGCCTTTCC CAAGGTGAAG 
ATCCAGATTC TAAGCAACTG GGGCCACCCC CGTTTCACGT GCTTGTATCG AGTCCGTGCC 
CACGGTGTGC GAACCTCAGA GGGGGCAGAG GGCAGTGCAC AGGGGCCCCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG4 ( NM_001317931.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304860.1 Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant 2, mRNA.

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CloneID	OHu53096
Clone ID Related Accession (Same CDS sequence)	NM_001317931.1
Accession Version	NM_001317931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	sperm-associated antigen 4 protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY021678.1 and BC143783.1. On Dec 15, 2015 this sequence version replaced XM_005260519.3. Transcript Variant: This variant (2) contains an alternate 5' terminal exon, resulting in a distinct 5' UTR and the use of an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus, and is shorter, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC143783.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGGA AGCTCTCAGC AGAAGCCAGC GCCTCGGAGC CACAACTGGC AGACAGCCTG 
TGGCGCGGCA ACCAACCGAC TGGGTCTCCA GTAGTCTCTG AGGAGCCGCT CGACCTTCTC 
CCGACCCTGG ATCTGAGGCA GGAGATGCCT CCCCCGCGGG TGTTCAAGAG CTTTCTGAGC 
CTGCTCTTCC AGGGGCTGAG CGTGTTGTTA TCCCTGGCAG GAGACGTGCT GGTCAGCATG 
TACAGGGAGG TCTGTTCCAT CCGCTTCCTG TTCACGGCTG TGTCGCTGCT GAGCCTCTTT 
CTGTCAGCAT TCTGGCTGGG GCTTCTGTAC CTGGTCTCTC CTTTGGAGAA TGAACCTAAG 
GAGATGCTGA CTCTAAGTGA GTACCACGAG CGCGTGCGCT CCCAGGGGCA GCAGCTGCAG 
CAGCTCCAGG CCGAGCTGGA TAAACTCCAC AAGGAGGTGT CCACTGTTCG GGCAGCCAAC 
AGCGAGAGAG TGGCCAAGCT CGTGTTCCAG AGGCTGAATG AGGATTTTGT GCGGAAGCCC 
GACTATGCTT TGAGCTCTGT GGGAGCCTCC ATCGACCTGC AGAAGACATC CCACGATTAC 
GCAGACAGGA ACACTGCCTA CTTCTGGAAT CGCTTCAGCT TCTGGAACTA CGCACGGCCG 
CCCACGGTTA TCCTGGAGCC CCACGTGTTC CCTGGGAATT GCTGGGCTTT TGAAGGCGAC 
CAAGGCCAGG TGGTGATCCA ACTGCCGGGC CGAGTGCAGC TGAGCGACAT CACTCTGCAG 
CATCCACCGC CCAGCGTGGA GCACACCGGA GGAGCCAACA GCGCCCCCCG CGATTTCGCG 
GTCTTTGGCC TCCAGGTTTA TGATGAAACT GAAGTTTCCT TGGGGAAATT CACCTTCGAT 
GTTGAGAAAT CGGAGATTCA GACTTTCCAC CTGCAGAATG ACCCCCCAGC TGCCTTTCCC 
AAGGTGAAGA TCCAGATTCT AAGCAACTGG GGCCACCCCC GTTTCACGTG CTTGTATCGA 
GTCCGTGCCC ACGGTGTGCG AACCTCAGAG GGGGCAGAGG GCAGTGCACA GGGGCCCCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304860.1
CDS	333..1415
Translation	MGRKLSAEASASEPQLADSLWRGNQPTGSPVVSEEPLDLLPTLDLRQEMPPPRVFKSFLSLLFQGLSVLLSLAGDVLVSMYREVCSIRFLFTAVSLLSLFLSAFWLGLLYLVSPLENEPKEMLTLSEYHERVRSQGQQLQQLQAELDKLHKEVSTVRAANSERVAKLVFQRLNEDFVRKPDYALSSVGASIDLQKTSHDYADRNTAYFWNRFSFWNYARPPTVILEPHVFPGNCWAFEGDQGQVVIQLPGRVQLSDITLQHPPPSVEHTGGANSAPRDFAVFGLQVYDETEVSLGKFTFDVEKSEIQTFHLQNDPPAAFPKVKIQILSNWGHPRFTCLYRVRAHGVRTSEGAEGSAQGPH

Target ORF information:

RefSeq Version	NM_001317931.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317931.1

ORF Insert Sequence:

ATGGGCAGGA AGCTCTCAGC AGAAGCCAGC GCCTCGGAGC CACAACTGGC AGACAGCCTG 
TGGCGCGGCA ACCAACCGAC TGGGTCTCCA GTAGTCTCTG AGGAGCCGCT CGACCTTCTC 
CCGACCCTGG ATCTGAGGCA GGAGATGCCT CCCCCGCGGG TGTTCAAGAG CTTTCTGAGC 
CTGCTCTTCC AGGGGCTGAG CGTGTTGTTA TCCCTGGCAG GAGACGTGCT GGTCAGCATG 
TACAGGGAGG TCTGTTCCAT CCGCTTCCTG TTCACGGCTG TGTCGCTGCT GAGCCTCTTT 
CTGTCAGCAT TCTGGCTGGG GCTTCTGTAC CTGGTCTCTC CTTTGGAGAA TGAACCTAAG 
GAGATGCTGA CTCTAAGTGA GTACCACGAG CGCGTGCGCT CCCAGGGGCA GCAGCTGCAG 
CAGCTCCAGG CCGAGCTGGA TAAACTCCAC AAGGAGGTGT CCACTGTTCG GGCAGCCAAC 
AGCGAGAGAG TGGCCAAGCT CGTGTTCCAG AGGCTGAATG AGGATTTTGT GCGGAAGCCC 
GACTATGCTT TGAGCTCTGT GGGAGCCTCC ATCGACCTGC AGAAGACATC CCACGATTAC 
GCAGACAGGA ACACTGCCTA CTTCTGGAAT CGCTTCAGCT TCTGGAACTA CGCACGGCCG 
CCCACGGTTA TCCTGGAGCC CCACGTGTTC CCTGGGAATT GCTGGGCTTT TGAAGGCGAC 
CAAGGCCAGG TGGTGATCCA ACTGCCGGGC CGAGTGCAGC TGAGCGACAT CACTCTGCAG 
CATCCACCGC CCAGCGTGGA GCACACCGGA GGAGCCAACA GCGCCCCCCG CGATTTCGCG 
GTCTTTGGCC TCCAGGTTTA TGATGAAACT GAAGTTTCCT TGGGGAAATT CACCTTCGAT 
GTTGAGAAAT CGGAGATTCA GACTTTCCAC CTGCAGAATG ACCCCCCAGC TGCCTTTCCC 
AAGGTGAAGA TCCAGATTCT AAGCAACTGG GGCCACCCCC GTTTCACGTG CTTGTATCGA 
GTCCGTGCCC ACGGTGTGCG AACCTCAGAG GGGGCAGAGG GCAGTGCACA GGGGCCCCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG4 ( XM_005260520.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005260577.1 Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant X2, mRNA.

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CloneID	OHu53097
Clone ID Related Accession (Same CDS sequence)	XM_005260520.4
Accession Version	XM_005260520.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 4 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260520.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATCTG GGCACCTCCT ACAAGAACCG ACTGGGTCTC CAGTAGTCTC TGAGGAGCCG 
CTCGACCTTC TCCCGACCCT GGATCTGAGG CAGGAGATGC CTCCCCCGCG GGTGTTCAAG 
AGCTTTCTGA GCCTGCTCTT CCAGGGGCTG AGCGTGTTGT TATCCCTGGC AGGAGACGTG 
CTGGTCAGCA TGTACAGGGA GGTCTGTTCC ATCCGCTTCC TGTTCACGGC TGTGTCGCTG 
CTGAGCCTCT TTCTGTCAGC ATTCTGGCTG GGGCTTCTGT ACCTGGTCTC TCCTTTGGAG 
AATGAACCTA AGGAGATGCT GACTCTAAGT GAGTACCACG AGCGCGTGCG CTCCCAGGGG 
CAGCAGCTGC AGCAGCTCCA GGCCGAGCTG GATAAACTCC ACAAGGAGGT GTCCACTGTT 
CGGGCAGCCA ACAGCGAGAG AGTGGCCAAG CTCGTGTTCC AGAGGCTGAA TGAGGATTTT 
GTGCGGAAGC CCGACTATGC TTTGAGCTCT GTGGGAGCCT CCATCGACCT GCAGAAGACA 
TCCCACGATT ACGCAGACAG GAACACTGCC TACTTCTGGA ATCGCTTCAG CTTCTGGAAC 
TACGCACGGC CGCCCACGGT TATCCTGGAG CCCCACGTGT TCCCTGGGAA TTGCTGGGCT 
TTTGAAGGCG ACCAAGGCCA GGTGGTGATC CAACTGCCGG GCCGAGTGCA GCTGAGCGAC 
ATCACTCTGC AGCATCCACC GCCCAGCGTG GAGCACACCG GAGGAGCCAA CAGCGCCCCC 
CGCGATTTCG CGGTCTTTGG CCTCCAGGTT TATGATGAAA CTGAAGTTTC CTTGGGGAAA 
TTCACCTTCG ATGTTGAGAA ATCGGAGATT CAGACTTTCC ACCTGCAGAA TGACCCCCCA 
GCTGCCTTTC CCAAGGTGAA GATCCAGATT CTAAGCAACT GGGGCCACCC CCGTTTCACG 
TGCTTGTATC GAGTCCGTGC CCACGGTGTG CGAACCTCAG AGGGGGCAGA GGGCAGTGCA 
CAGGGGCCCC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005260577.1
CDS	46..1080
Translation	MISGHLLQEPTGSPVVSEEPLDLLPTLDLRQEMPPPRVFKSFLSLLFQGLSVLLSLAGDVLVSMYREVCSIRFLFTAVSLLSLFLSAFWLGLLYLVSPLENEPKEMLTLSEYHERVRSQGQQLQQLQAELDKLHKEVSTVRAANSERVAKLVFQRLNEDFVRKPDYALSSVGASIDLQKTSHDYADRNTAYFWNRFSFWNYARPPTVILEPHVFPGNCWAFEGDQGQVVIQLPGRVQLSDITLQHPPPSVEHTGGANSAPRDFAVFGLQVYDETEVSLGKFTFDVEKSEIQTFHLQNDPPAAFPKVKIQILSNWGHPRFTCLYRVRAHGVRTSEGAEGSAQGPH

Target ORF information:

RefSeq Version	XM_005260520.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260520.4

ORF Insert Sequence:

ATGATATCTG GGCACCTCCT ACAAGAACCG ACTGGGTCTC CAGTAGTCTC TGAGGAGCCG 
CTCGACCTTC TCCCGACCCT GGATCTGAGG CAGGAGATGC CTCCCCCGCG GGTGTTCAAG 
AGCTTTCTGA GCCTGCTCTT CCAGGGGCTG AGCGTGTTGT TATCCCTGGC AGGAGACGTG 
CTGGTCAGCA TGTACAGGGA GGTCTGTTCC ATCCGCTTCC TGTTCACGGC TGTGTCGCTG 
CTGAGCCTCT TTCTGTCAGC ATTCTGGCTG GGGCTTCTGT ACCTGGTCTC TCCTTTGGAG 
AATGAACCTA AGGAGATGCT GACTCTAAGT GAGTACCACG AGCGCGTGCG CTCCCAGGGG 
CAGCAGCTGC AGCAGCTCCA GGCCGAGCTG GATAAACTCC ACAAGGAGGT GTCCACTGTT 
CGGGCAGCCA ACAGCGAGAG AGTGGCCAAG CTCGTGTTCC AGAGGCTGAA TGAGGATTTT 
GTGCGGAAGC CCGACTATGC TTTGAGCTCT GTGGGAGCCT CCATCGACCT GCAGAAGACA 
TCCCACGATT ACGCAGACAG GAACACTGCC TACTTCTGGA ATCGCTTCAG CTTCTGGAAC 
TACGCACGGC CGCCCACGGT TATCCTGGAG CCCCACGTGT TCCCTGGGAA TTGCTGGGCT 
TTTGAAGGCG ACCAAGGCCA GGTGGTGATC CAACTGCCGG GCCGAGTGCA GCTGAGCGAC 
ATCACTCTGC AGCATCCACC GCCCAGCGTG GAGCACACCG GAGGAGCCAA CAGCGCCCCC 
CGCGATTTCG CGGTCTTTGG CCTCCAGGTT TATGATGAAA CTGAAGTTTC CTTGGGGAAA 
TTCACCTTCG ATGTTGAGAA ATCGGAGATT CAGACTTTCC ACCTGCAGAA TGACCCCCCA 
GCTGCCTTTC CCAAGGTGAA GATCCAGATT CTAAGCAACT GGGGCCACCC CCGTTTCACG 
TGCTTGTATC GAGTCCGTGC CCACGGTGTG CGAACCTCAG AGGGGGCAGA GGGCAGTGCA 
CAGGGGCCCC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG4 ( XM_011529009.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527311.1 Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu60701
Clone ID Related Accession (Same CDS sequence)	XM_011529009.2
Accession Version	XM_011529009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 4 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011529009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGAA GCTCCCGCCC GGGCTCGGCC TCGTCCTCGC GCAAGCACAC GCCCAACTTT 
TTCAGCGAGA ACAGCTCAAT GAGCATCACC TCGGAGGACA GCAAAGGGCT CCGGTCAGCG 
GAGCCCGGGC CTGGGGAGCC CGAGGGCAGA AGAGCCCGGG GCCCGAGCTG CGAACCGACT 
GGGTCTCCAG TAGTCTCTGA GGAGCCGCTC GACCTTCTCC CGACCCTGGA TCTGAGGCAG 
GAGATGCCTC CCCCGCGGGT GTTCAAGAGC TTTCTGAGCC TGCTCTTCCA GGGGCTGAGC 
GTGTTGTTAT CCCTGGCAGG AGACGTGCTG GTCAGCATGT ACAGGGAGGT CTGTTCCATC 
CGCTTCCTGT TCACGGCTGT GTCGCTGCTG AGCCTCTTTC TGTCAGCATT CTGGCTGGGG 
CTTCTGTACC TGGTCTCTCC TTTGGAGAAT GAACCTAAGG AGATGCTGAC TCTAAGTGAG 
TACCACGAGC GCGTGCGCTC CCAGGGGCAG CAGCTGCAGC AGCTCCAGGC CGAGCTGGAT 
AAACTCCACA AGGAGGTGTC CACTGTTCGG GCAGCCAACA GCGAGAGAGT GGCCAAGCTC 
GTGTTCCAGA GGCTGAATGA GGATTTTGTG CGGAAGCCCG ACTATGCTTT GAGCTCTGTG 
GGAGCCTCCA TCGACCTGCA GAAGACATCC CACGATTACG CAGACAGGAA CACTGCCTAC 
TTCTGGAATC GCTTCAGCTT CTGGAACTAC GCACGGCCGC CCACGGTTAT CCTGGAGCCC 
CACGTGTTCC CTGGGAATTG CTGGGCTTTT GAAGGCGACC AAGGCCAGGT GGTGATCCAA 
CTGCCGGGCC GAGTGCAGCT GAGCGACATC ACTCTGCAGC ATCCACCGCC CAGCGTGGAG 
CACACCGGAG GAGCCAACAG CGCCCCCCGC GATTTCGCGG TCTTTGGCCT CCAGGTTTAT 
GATGAAACTG AAGTTTCCTT GGGGAAATTC ACCTTCGATG TTGAGAAATC GGAGATTCAG 
ACTTTCCACC TGCAGAATGA CCCCCCAGCT GCCTTTCCCA AGGTGAAGAT CCAGATTCTA 
AGCAACTGGG GCCACCCCCG TTTCACGTGC TTGTATCGAG TCCGTGCCCA CGGTGTGCGA 
ACCTCAGAGG GGGCAGAGGG CAGTGCACAG GGGCCCCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527311.1
CDS	149..1330
Translation	MRRSSRPGSASSSRKHTPNFFSENSSMSITSEDSKGLRSAEPGPGEPEGRRARGPSCEPTGSPVVSEEPLDLLPTLDLRQEMPPPRVFKSFLSLLFQGLSVLLSLAGDVLVSMYREVCSIRFLFTAVSLLSLFLSAFWLGLLYLVSPLENEPKEMLTLSEYHERVRSQGQQLQQLQAELDKLHKEVSTVRAANSERVAKLVFQRLNEDFVRKPDYALSSVGASIDLQKTSHDYADRNTAYFWNRFSFWNYARPPTVILEPHVFPGNCWAFEGDQGQVVIQLPGRVQLSDITLQHPPPSVEHTGGANSAPRDFAVFGLQVYDETEVSLGKFTFDVEKSEIQTFHLQNDPPAAFPKVKIQILSNWGHPRFTCLYRVRAHGVRTSEGAEGSAQGPH

Target ORF information:

RefSeq Version	XM_011529009.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 4 (SPAG4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529009.2

ORF Insert Sequence:

ATGCGGCGAA GCTCCCGCCC GGGCTCGGCC TCGTCCTCGC GCAAGCACAC GCCCAACTTT 
TTCAGCGAGA ACAGCTCAAT GAGCATCACC TCGGAGGACA GCAAAGGGCT CCGGTCAGCG 
GAGCCCGGGC CTGGGGAGCC CGAGGGCAGA AGAGCCCGGG GCCCGAGCTG CGAACCGACT 
GGGTCTCCAG TAGTCTCTGA GGAGCCGCTC GACCTTCTCC CGACCCTGGA TCTGAGGCAG 
GAGATGCCTC CCCCGCGGGT GTTCAAGAGC TTTCTGAGCC TGCTCTTCCA GGGGCTGAGC 
GTGTTGTTAT CCCTGGCAGG AGACGTGCTG GTCAGCATGT ACAGGGAGGT CTGTTCCATC 
CGCTTCCTGT TCACGGCTGT GTCGCTGCTG AGCCTCTTTC TGTCAGCATT CTGGCTGGGG 
CTTCTGTACC TGGTCTCTCC TTTGGAGAAT GAACCTAAGG AGATGCTGAC TCTAAGTGAG 
TACCACGAGC GCGTGCGCTC CCAGGGGCAG CAGCTGCAGC AGCTCCAGGC CGAGCTGGAT 
AAACTCCACA AGGAGGTGTC CACTGTTCGG GCAGCCAACA GCGAGAGAGT GGCCAAGCTC 
GTGTTCCAGA GGCTGAATGA GGATTTTGTG CGGAAGCCCG ACTATGCTTT GAGCTCTGTG 
GGAGCCTCCA TCGACCTGCA GAAGACATCC CACGATTACG CAGACAGGAA CACTGCCTAC 
TTCTGGAATC GCTTCAGCTT CTGGAACTAC GCACGGCCGC CCACGGTTAT CCTGGAGCCC 
CACGTGTTCC CTGGGAATTG CTGGGCTTTT GAAGGCGACC AAGGCCAGGT GGTGATCCAA 
CTGCCGGGCC GAGTGCAGCT GAGCGACATC ACTCTGCAGC ATCCACCGCC CAGCGTGGAG 
CACACCGGAG GAGCCAACAG CGCCCCCCGC GATTTCGCGG TCTTTGGCCT CCAGGTTTAT 
GATGAAACTG AAGTTTCCTT GGGGAAATTC ACCTTCGATG TTGAGAAATC GGAGATTCAG 
ACTTTCCACC TGCAGAATGA CCCCCCAGCT GCCTTTCCCA AGGTGAAGAT CCAGATTCTA 
AGCAACTGGG GCCACCCCCG TTTCACGTGC TTGTATCGAG TCCGTGCCCA CGGTGTGCGA 
ACCTCAGAGG GGGCAGAGGG CAGTGCACAG GGGCCCCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SEPT12/SPAG4/LAMINB1 complexes are required for maintaining the integrity of the nuclear envelope in postmeiotic male germ cells.
PloS one10(3)e0120722(2015)
Yeh CH,Kuo PL,Wang YY,Wu YY,Chen MF,Lin DY,Lai TH,Chiang HS,Lin YH

Hypoxia regulates the sperm associated antigen 4 (SPAG4) via HIF, which is expressed in renal clear cell carcinoma and promotes migration and invasion in vitro.
Molecular carcinogenesis53(12)970-8(2014 Dec)
Knaup KX,Monti J,Hackenbeck T,Jobst-Schwan T,Klanke B,Schietke RE,Wacker I,Behrens J,Amann K,Eckardt KU,Warnecke C,Wiesener MS

Sperm-associated antigen 4, a novel hypoxia-inducible factor 1 target, regulates cytokinesis, and its expression correlates with the prognosis of renal cell carcinoma.
The American journal of pathology182(6)2191-203(2013 Jun)
Shoji K,Murayama T,Mimura I,Wada T,Kume H,Goto A,Ohse T,Tanaka T,Inagi R,van der Hoorn FA,Manabe I,Homma Y,Fukayama M,Sakurai T,Hasegawa T,Aburatani H,Kodama T,Nangaku M

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SPAG4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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