MICAL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MICAL1
Entrez Gene ID	64780
Full Name	microtubule associated monooxygenase, calponin and LIM domain containing 1
Synonyms	MICAL,MICAL-1,NICAL
General protein information	Preferred Names microtubule associated monooxygenase, calponin and LIM domain containing 1 Names [F-actin]-monooxygenase MICAL1 NEDD9-interacting protein with calponin homology and LIM domains [F-actin]-methionine sulfoxide oxidase MICAL1 molecule interacting with CasL protein 1 protein-methionine sulfoxide oxidase MICAL1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q21
Summary	This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015].
Disorder MIM:	607129

mRNA and Protein(s)

mRNA	Protein	Name
NM_001159291.1	NP_001152763.1	[F-actin]-monooxygenase MICAL1 isoform 2
NM_022765.4	NP_073602.3	[F-actin]-monooxygenase MICAL1 isoform 1
NM_001286613.1	NP_001273542.1	[F-actin]-monooxygenase MICAL1 isoform 3
NM_022765.3	NP_073602.3	[F-actin]-monooxygenase MICAL1 isoform 1

Pathways from BioSystems

Homologies

	MICAL1	XP_001090339.1
Homo sapiens (human)	MICAL1	NP_001273542.1
Pan troglodytes (chimpanzee)	MICAL1	XP_001153132.2
Rattus norvegicus (Norway rat)	Mical1	NP_001099867.1
Canis lupus familiaris (dog)	MICAL1	XP_005627842.1
Mus musculus (house mouse)	Mical1	NP_612188.1
Gallus gallus (chicken)	MICAL1	NP_001026747.1
Bos taurus (cattle)	MICAL1	NP_001075051.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure-function studies of MICAL, the unusual multidomain flavoenzyme involved in actin cytoskeleton dynamics.
Vanoni MA
Archives of biochemistry and biophysics632118-141(2017 Oct)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

MICAL1 controls cell invasive phenotype via regulating oxidative stress in breast cancer cells.
Deng W, Wang Y, Gu L, Duan B, Cui J, Zhang Y, Chen Y, Sun S, Dong J, Du J
BMC cancer16489(2016 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results provide evidence that MICAL1 plays an essential role in the activation of ROS/Akt signaling and cell invasive phenotype and identify a novel link between RAB35 and MICAL1 in regulating breast cancer cell invasion.
Title: MICAL1 controls cell invasive phenotype via regulating oxidative stress in breast cancer cells.

Data suggest that Sema6A and Mical1 may represent new potential therapeutic targets in BRAFV600E melanoma.
Title: Sema6A and Mical1 control cell growth and survival of BRAFV600E human melanoma cells.

The MICAL1 directly induce oxidation of actin molecules, leading to actin depolymerization. ROS production by MICAL1 also causes oxidation of collapsin response mediator protein-2 which subsequently undergoes phosphorylation.
Title: MICAL-family proteins: Complex regulators of the actin cytoskeleton.

although MICAL1 is auto-inhibited by its C-terminal coiled-coil region, MICAL2 remains constitutively active and affects stress fibers, suggesting differential but complementary roles for MICAL1 and MICAL2 in actin microfilament regulation
Title: Differential regulation of actin microfilaments by human MICAL proteins.

Findings show a biological role for MICAL-1 in apoptosis and define a novel negative regulatory mechanism of MST-NDR signaling.
Title: MICAL-1 is a negative regulator of MST-NDR kinase signaling and apoptosis.

The following MICAL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MICAL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26204	NM_022765.4 Latest version!	Homo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu26666	NM_001159291.1 Latest version!	Homo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu12214	NM_001286613.1 Latest version!	Homo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu26204	NM_022765.3	Homo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MICAL1 ( NM_022765.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_073602.3 Homo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26204
Clone ID Related Accession (Same CDS sequence)	NM_022765.4 , NM_022765.3
Accession Version	NM_022765.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	[F-actin]-monooxygenase MICAL1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC042144.1. On Nov 23, 2018 this sequence version replaced NM_022765.3. Transcript Variant: This variant (1) contains an alternate 5' exon and thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 3. The encoded isoform (1, also known as MICAL-1a) is shorter at the N-terminus, compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC042144.1, AB048948.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000358807.8/ ENSP00000351664.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTTCAC CTACCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CCTGCAGGCC 
CAGCTGTGCC AGGACGTGCT GAGCAGCTTC CAGGAGCTGT GTGGGGCCCT GGGGCTGGAA 
CCCGGTGGGG GGCTGCCCCA GTACCACAAG ATCAAGGACC AGCTCAACTA CTGGAGCGCC 
AAGTCACTGT GGACCAAGCT GGACAAGCGA GCAGGCCAGC CTGTCTACCA GCAGGGCCGG 
GCCTGCACCA GCACCAAGTG CCTGGTGGTG GGTGCTGGAC CTTGCGGGCT GCGGGTCGCT 
GTGGAGCTGG CGCTGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTGCGGGC ACTCGGTGCT 
AAGAAGTTCT ACGGGCGCTT CTGCACCGGC ACCCTGGACC ACATCAGCAT CAGGCAGCTC 
CAGCTGCTTC TGCTGAAGGT AGCATTGCTG CTGGGGGTGG AAATTCACTG GGGTGTCACT 
TTCACTGGCC TCCAGCCCCC TCCTAGGAAG GGGAGTGGCT GGCGTGCCCA GCTCCAACCC 
AACCCCCCTG CCCAGCTGGC CAACTATGAA TTTGACGTCC TTATCTCGGC TGCAGGAGGT 
AAATTCGTCC CTGAAGGCTT CAAAGTTCGA GAAATGCGAG GCAAACTGGC CATTGGCATC 
ACAGCCAACT TTGTGAATGG ACGCACCGTG GAGGAGACAC AGGTGCCGGA GATCAGTGGT 
GTAGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTTC TCAAAGCCAC AGGCATTGAT 
CTGGAGAACA TTGTGTACTA CAAGGACGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TGCGGCTGGG GGTGCTGCGC CAGGACTGGC CAGACACCAA TCGGCTGCTG 
GGCAGTGCCA ATGTGGTGCC CGAGGCTCTG CAGCGCTTTA CCCGGGCAGC TGCTGACTTT 
GCCACCCATG GCAAGCTCGG GAAACTAGAG TTTGCCCAGG ATGCCCATGG GCAGCCTGAT 
GTCTCTGCCT TTGACTTCAC GAGCATGATG CGGGCAGAGA GTTCTGCTCG TGTGCAAGAG 
AAGCATGGCG CCCGCCTGCT GCTGGGACTG GTGGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC ACGGGGCTTC CTGGCAGCCT TTGATGCAGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGAGTCCCTA GAGGTGTTGG CTGAGCGTGA GAGCCTGTAC 
CAGCTTCTGT CACAGACATC CCCAGAAAAC ATGCATCGCA ATGTGGCCCA GTATGGGCTG 
GACCCAGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCAG TGACCCCCAA TCAGGTACGA 
GACCTGTATG ATGTGCTAGC CAAGGAGCCT GTGCAGAGGA ACAACGACAA GACAGATACA 
GGGATGCCAG CCACCGGGTC GGCAGGCACC CAGGAGGAGC TGCTACGCTG GTGCCAGGAG 
CAGACAGCTG GGTACCCGGG AGTCCACGTC TCCGATTTGT CTTCCTCCTG GGCTGATGGG 
CTAGCTCTGT GTGCCCTGGT GTACCGGCTG CAGCCTGGCC TGCTGGAACC CTCAGAGCTG 
CAGGGGCTGG GAGCTCTGGA AGCAACTGCT TGGGCACTAA AGGTGGCAGA GAATGAGCTG 
GGCATCACAC CGGTGGTGTC TGCACAGGCC GTGGTAGCAG GGAGTGACCC ACTGGGCCTC 
ATTGCCTACC TCAGCCACTT CCACAGTGCC TTCAAGAGCA TGGCCCACAG CCCAGGCCCT 
GTCAGCCAGG CCTCCCCAGG GACCTCCAGT GCTGTATTAT TCCTTAGTAA ACTTCAGAGG 
ACCCTGCAGC GATCCCGGGC CAAGGAAAAT GCAGAGGATG CTGGTGGCAA GAAGCTGCGC 
TTGGAGATGG AGGCCGAGAC CCCAAGTACT GAGGTGCCAC CTGACCCAGA GCCTGGTGTA 
CCCCTGACAC CCCCATCCCA ACACCAGGAG GCCGGTGCTG GGGACCTGTG TGCACTTTGT 
GGGGAACACC TCTATGTCCT GGAACGCCTC TGTGTCAACG GCCATTTCTT CCACCGGAGC 
TGCTTCCGCT GCCATACCTG TGAGGCCACA CTGTGGCCAG GTGGCTACGA GCAGCACCCA 
GGAGATGGAC ATTTCTACTG CCTCCAGCAC CTGCCCCAGA CAGACCACAA AGCGGAAGGC 
AGCGATAGAG GCCCTGAGAG TCCGGAGCTC CCCACACCAA GTGAGAATAG CATGCCACCA 
GGCCTCTCAA CTCCCACAGC CTCGCAGGAG GGGGCCGGTC CTGTTCCAGA TCCCAGCCAG 
CCCACCCGTC GGCAGATCCG CCTCTCCAGC CCGGAGCGCC AGCGGTTGTC CTCCCTTAAC 
CTTACCCCTG ACCCGGAAAT GGAGCCTCCA CCCAAGCCTC CCCGCAGCTG CTCCGCCTTG 
GCCCGCCACG CCCTGGAGAG CAGCTTTGTG GGCTGGGGCC TGCCAGTCCA GAGCCCTCAA 
GCTCTTGTGG CCATGGAGAA GGAGGAAAAA GAGAGTCCCT TCTCCAGTGA AGAGGAAGAA 
GAAGATGTGC CTTTGGACTC AGATGTGGAA CAGGCCCTGC AGACCTTTGC CAAGACCTCA 
GGCACCATGA ATAACTACCC AACATGGCGT CGGACTCTGC TGCGCCGTGC GAAGGAGGAG 
GAGATGAAGA GGTTCTGCAA GGCCCAGACC ATCCAACGGC GACTAAATGA GATTGAGGCT 
GCCTTGAGGG AGCTAGAGGC CGAGGGCGTG AAGCTGGAGC TGGCCTTGAG GCGCCAGAGC 
AGTTCCCCAG AACAGCAAAA GAAACTATGG GTAGGACAGC TGCTACAGCT CGTTGACAAG 
AAAAACAGCC TGGTGGCTGA GGAGGCCGAG CTCATGATCA CGGTGCAGGA ATTGAATCTG 
GAGGAGAAAC AGTGGCAGCT GGACCAGGAG CTACGAGGCT ACATGAACCG GGAAGAAAAC 
CTAAAGACAG CTGCTGATCG GCAGGCTGAG GACCAGGTCC TGAGGAAGCT GGTGGATTTG 
GTCAACCAGA GAGATGCCCT CATCCGCTTC CAGGAGGAGC GCAGGCTCAG CGAGCTGGCC 
TTGGGGACAG GGGCCCAGGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073602.3
CDS	98..3301
Translation	MASPTSTNPAHAHFESFLQAQLCQDVLSSFQELCGALGLEPGGGLPQYHKIKDQLNYWSAKSLWTKLDKRAGQPVYQQGRACTSTKCLVVGAGPCGLRVAVELALLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGAKKFYGRFCTGTLDHISIRQLQLLLLKVALLLGVEIHWGVTFTGLQPPPRKGSGWRAQLQPNPPAQLANYEFDVLISAAGGKFVPEGFKVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGIDLENIVYYKDDTHYFVMTAKKQCLLRLGVLRQDWPDTNRLLGSANVVPEALQRFTRAAADFATHGKLGKLEFAQDAHGQPDVSAFDFTSMMRAESSARVQEKHGARLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMVKRWAEGAESLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPNQVRDLYDVLAKEPVQRNNDKTDTGMPATGSAGTQEELLRWCQEQTAGYPGVHVSDLSSSWADGLALCALVYRLQPGLLEPSELQGLGALEATAWALKVAENELGITPVVSAQAVVAGSDPLGLIAYLSHFHSAFKSMAHSPGPVSQASPGTSSAVLFLSKLQRTLQRSRAKENAEDAGGKKLRLEMEAETPSTEVPPDPEPGVPLTPPSQHQEAGAGDLCALCGEHLYVLERLCVNGHFFHRSCFRCHTCEATLWPGGYEQHPGDGHFYCLQHLPQTDHKAEGSDRGPESPELPTPSENSMPPGLSTPTASQEGAGPVPDPSQPTRRQIRLSSPERQRLSSLNLTPDPEMEPPPKPPRSCSALARHALESSFVGWGLPVQSPQALVAMEKEEKESPFSSEEEEEDVPLDSDVEQALQTFAKTSGTMNNYPTWRRTLLRRAKEEEMKRFCKAQTIQRRLNEIEAALRELEAEGVKLELALRRQSSSPEQQKKLWVGQLLQLVDKKNSLVAEEAELMITVQELNLEEKQWQLDQELRGYMNREENLKTAADRQAEDQVLRKLVDLVNQRDALIRFQEERRLSELALGTGAQG

Target ORF information:

RefSeq Version	NM_022765.4
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022765.4

ORF Insert Sequence:

ATGGCTTCAC CTACCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CCTGCAGGCC 
CAGCTGTGCC AGGACGTGCT GAGCAGCTTC CAGGAGCTGT GTGGGGCCCT GGGGCTGGAA 
CCCGGTGGGG GGCTGCCCCA GTACCACAAG ATCAAGGACC AGCTCAACTA CTGGAGCGCC 
AAGTCACTGT GGACCAAGCT GGACAAGCGA GCAGGCCAGC CTGTCTACCA GCAGGGCCGG 
GCCTGCACCA GCACCAAGTG CCTGGTGGTG GGTGCTGGAC CTTGCGGGCT GCGGGTCGCT 
GTGGAGCTGG CGCTGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTGCGGGC ACTCGGTGCT 
AAGAAGTTCT ACGGGCGCTT CTGCACCGGC ACCCTGGACC ACATCAGCAT CAGGCAGCTC 
CAGCTGCTTC TGCTGAAGGT AGCATTGCTG CTGGGGGTGG AAATTCACTG GGGTGTCACT 
TTCACTGGCC TCCAGCCCCC TCCTAGGAAG GGGAGTGGCT GGCGTGCCCA GCTCCAACCC 
AACCCCCCTG CCCAGCTGGC CAACTATGAA TTTGACGTCC TTATCTCGGC TGCAGGAGGT 
AAATTCGTCC CTGAAGGCTT CAAAGTTCGA GAAATGCGAG GCAAACTGGC CATTGGCATC 
ACAGCCAACT TTGTGAATGG ACGCACCGTG GAGGAGACAC AGGTGCCGGA GATCAGTGGT 
GTAGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTTC TCAAAGCCAC AGGCATTGAT 
CTGGAGAACA TTGTGTACTA CAAGGACGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TGCGGCTGGG GGTGCTGCGC CAGGACTGGC CAGACACCAA TCGGCTGCTG 
GGCAGTGCCA ATGTGGTGCC CGAGGCTCTG CAGCGCTTTA CCCGGGCAGC TGCTGACTTT 
GCCACCCATG GCAAGCTCGG GAAACTAGAG TTTGCCCAGG ATGCCCATGG GCAGCCTGAT 
GTCTCTGCCT TTGACTTCAC GAGCATGATG CGGGCAGAGA GTTCTGCTCG TGTGCAAGAG 
AAGCATGGCG CCCGCCTGCT GCTGGGACTG GTGGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC ACGGGGCTTC CTGGCAGCCT TTGATGCAGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGAGTCCCTA GAGGTGTTGG CTGAGCGTGA GAGCCTGTAC 
CAGCTTCTGT CACAGACATC CCCAGAAAAC ATGCATCGCA ATGTGGCCCA GTATGGGCTG 
GACCCAGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCAG TGACCCCCAA TCAGGTACGA 
GACCTGTATG ATGTGCTAGC CAAGGAGCCT GTGCAGAGGA ACAACGACAA GACAGATACA 
GGGATGCCAG CCACCGGGTC GGCAGGCACC CAGGAGGAGC TGCTACGCTG GTGCCAGGAG 
CAGACAGCTG GGTACCCGGG AGTCCACGTC TCCGATTTGT CTTCCTCCTG GGCTGATGGG 
CTAGCTCTGT GTGCCCTGGT GTACCGGCTG CAGCCTGGCC TGCTGGAACC CTCAGAGCTG 
CAGGGGCTGG GAGCTCTGGA AGCAACTGCT TGGGCACTAA AGGTGGCAGA GAATGAGCTG 
GGCATCACAC CGGTGGTGTC TGCACAGGCC GTGGTAGCAG GGAGTGACCC ACTGGGCCTC 
ATTGCCTACC TCAGCCACTT CCACAGTGCC TTCAAGAGCA TGGCCCACAG CCCAGGCCCT 
GTCAGCCAGG CCTCCCCAGG GACCTCCAGT GCTGTATTAT TCCTTAGTAA ACTTCAGAGG 
ACCCTGCAGC GATCCCGGGC CAAGGAAAAT GCAGAGGATG CTGGTGGCAA GAAGCTGCGC 
TTGGAGATGG AGGCCGAGAC CCCAAGTACT GAGGTGCCAC CTGACCCAGA GCCTGGTGTA 
CCCCTGACAC CCCCATCCCA ACACCAGGAG GCCGGTGCTG GGGACCTGTG TGCACTTTGT 
GGGGAACACC TCTATGTCCT GGAACGCCTC TGTGTCAACG GCCATTTCTT CCACCGGAGC 
TGCTTCCGCT GCCATACCTG TGAGGCCACA CTGTGGCCAG GTGGCTACGA GCAGCACCCA 
GGAGATGGAC ATTTCTACTG CCTCCAGCAC CTGCCCCAGA CAGACCACAA AGCGGAAGGC 
AGCGATAGAG GCCCTGAGAG TCCGGAGCTC CCCACACCAA GTGAGAATAG CATGCCACCA 
GGCCTCTCAA CTCCCACAGC CTCGCAGGAG GGGGCCGGTC CTGTTCCAGA TCCCAGCCAG 
CCCACCCGTC GGCAGATCCG CCTCTCCAGC CCGGAGCGCC AGCGGTTGTC CTCCCTTAAC 
CTTACCCCTG ACCCGGAAAT GGAGCCTCCA CCCAAGCCTC CCCGCAGCTG CTCCGCCTTG 
GCCCGCCACG CCCTGGAGAG CAGCTTTGTG GGCTGGGGCC TGCCAGTCCA GAGCCCTCAA 
GCTCTTGTGG CCATGGAGAA GGAGGAAAAA GAGAGTCCCT TCTCCAGTGA AGAGGAAGAA 
GAAGATGTGC CTTTGGACTC AGATGTGGAA CAGGCCCTGC AGACCTTTGC CAAGACCTCA 
GGCACCATGA ATAACTACCC AACATGGCGT CGGACTCTGC TGCGCCGTGC GAAGGAGGAG 
GAGATGAAGA GGTTCTGCAA GGCCCAGACC ATCCAACGGC GACTAAATGA GATTGAGGCT 
GCCTTGAGGG AGCTAGAGGC CGAGGGCGTG AAGCTGGAGC TGGCCTTGAG GCGCCAGAGC 
AGTTCCCCAG AACAGCAAAA GAAACTATGG GTAGGACAGC TGCTACAGCT CGTTGACAAG 
AAAAACAGCC TGGTGGCTGA GGAGGCCGAG CTCATGATCA CGGTGCAGGA ATTGAATCTG 
GAGGAGAAAC AGTGGCAGCT GGACCAGGAG CTACGAGGCT ACATGAACCG GGAAGAAAAC 
CTAAAGACAG CTGCTGATCG GCAGGCTGAG GACCAGGTCC TGAGGAAGCT GGTGGATTTG 
GTCAACCAGA GAGATGCCCT CATCCGCTTC CAGGAGGAGC GCAGGCTCAG CGAGCTGGCC 
TTGGGGACAG GGGCCCAGGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL1 ( NM_022765.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_073602.3 Homo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26204
Clone ID Related Accession (Same CDS sequence)	NM_022765.4 , NM_022765.3
Accession Version	NM_022765.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-09
Organism	Homo sapiens(human)
Product	[F-actin]-monooxygenase MICAL1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB048948.1 and BC042144.1. On Sep 20, 2008 this sequence version replaced NM_022765.2. Transcript Variant: This variant (1) contains an alternate 5' exon and thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 3. The encoded isoform (1, also known as MICAL-1a) is shorter at the N-terminus, compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB048948.1, SRR1803615.1561.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCAC CTACCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CCTGCAGGCC 
CAGCTGTGCC AGGACGTGCT GAGCAGCTTC CAGGAGCTGT GTGGGGCCCT GGGGCTGGAA 
CCCGGTGGGG GGCTGCCCCA GTACCACAAG ATCAAGGACC AGCTCAACTA CTGGAGCGCC 
AAGTCACTGT GGACCAAGCT GGACAAGCGA GCAGGCCAGC CTGTCTACCA GCAGGGCCGG 
GCCTGCACCA GCACCAAGTG CCTGGTGGTG GGTGCTGGAC CTTGCGGGCT GCGGGTCGCT 
GTGGAGCTGG CGCTGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTGCGGGC ACTCGGTGCT 
AAGAAGTTCT ACGGGCGCTT CTGCACCGGC ACCCTGGACC ACATCAGCAT CAGGCAGCTC 
CAGCTGCTTC TGCTGAAGGT AGCATTGCTG CTGGGGGTGG AAATTCACTG GGGTGTCACT 
TTCACTGGCC TCCAGCCCCC TCCTAGGAAG GGGAGTGGCT GGCGTGCCCA GCTCCAACCC 
AACCCCCCTG CCCAGCTGGC CAACTATGAA TTTGACGTCC TTATCTCGGC TGCAGGAGGT 
AAATTCGTCC CTGAAGGCTT CAAAGTTCGA GAAATGCGAG GCAAACTGGC CATTGGCATC 
ACAGCCAACT TTGTGAATGG ACGCACCGTG GAGGAGACAC AGGTGCCGGA GATCAGTGGT 
GTAGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTTC TCAAAGCCAC AGGCATTGAT 
CTGGAGAACA TTGTGTACTA CAAGGACGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TGCGGCTGGG GGTGCTGCGC CAGGACTGGC CAGACACCAA TCGGCTGCTG 
GGCAGTGCCA ATGTGGTGCC CGAGGCTCTG CAGCGCTTTA CCCGGGCAGC TGCTGACTTT 
GCCACCCATG GCAAGCTCGG GAAACTAGAG TTTGCCCAGG ATGCCCATGG GCAGCCTGAT 
GTCTCTGCCT TTGACTTCAC GAGCATGATG CGGGCAGAGA GTTCTGCTCG TGTGCAAGAG 
AAGCATGGCG CCCGCCTGCT GCTGGGACTG GTGGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC ACGGGGCTTC CTGGCAGCCT TTGATGCAGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGAGTCCCTA GAGGTGTTGG CTGAGCGTGA GAGCCTGTAC 
CAGCTTCTGT CACAGACATC CCCAGAAAAC ATGCATCGCA ATGTGGCCCA GTATGGGCTG 
GACCCAGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCAG TGACCCCCAA TCAGGTACGA 
GACCTGTATG ATGTGCTAGC CAAGGAGCCT GTGCAGAGGA ACAACGACAA GACAGATACA 
GGGATGCCAG CCACCGGGTC GGCAGGCACC CAGGAGGAGC TGCTACGCTG GTGCCAGGAG 
CAGACAGCTG GGTACCCGGG AGTCCACGTC TCCGATTTGT CTTCCTCCTG GGCTGATGGG 
CTAGCTCTGT GTGCCCTGGT GTACCGGCTG CAGCCTGGCC TGCTGGAACC CTCAGAGCTG 
CAGGGGCTGG GAGCTCTGGA AGCAACTGCT TGGGCACTAA AGGTGGCAGA GAATGAGCTG 
GGCATCACAC CGGTGGTGTC TGCACAGGCC GTGGTAGCAG GGAGTGACCC ACTGGGCCTC 
ATTGCCTACC TCAGCCACTT CCACAGTGCC TTCAAGAGCA TGGCCCACAG CCCAGGCCCT 
GTCAGCCAGG CCTCCCCAGG GACCTCCAGT GCTGTATTAT TCCTTAGTAA ACTTCAGAGG 
ACCCTGCAGC GATCCCGGGC CAAGGAAAAT GCAGAGGATG CTGGTGGCAA GAAGCTGCGC 
TTGGAGATGG AGGCCGAGAC CCCAAGTACT GAGGTGCCAC CTGACCCAGA GCCTGGTGTA 
CCCCTGACAC CCCCATCCCA ACACCAGGAG GCCGGTGCTG GGGACCTGTG TGCACTTTGT 
GGGGAACACC TCTATGTCCT GGAACGCCTC TGTGTCAACG GCCATTTCTT CCACCGGAGC 
TGCTTCCGCT GCCATACCTG TGAGGCCACA CTGTGGCCAG GTGGCTACGA GCAGCACCCA 
GGAGATGGAC ATTTCTACTG CCTCCAGCAC CTGCCCCAGA CAGACCACAA AGCGGAAGGC 
AGCGATAGAG GCCCTGAGAG TCCGGAGCTC CCCACACCAA GTGAGAATAG CATGCCACCA 
GGCCTCTCAA CTCCCACAGC CTCGCAGGAG GGGGCCGGTC CTGTTCCAGA TCCCAGCCAG 
CCCACCCGTC GGCAGATCCG CCTCTCCAGC CCGGAGCGCC AGCGGTTGTC CTCCCTTAAC 
CTTACCCCTG ACCCGGAAAT GGAGCCTCCA CCCAAGCCTC CCCGCAGCTG CTCCGCCTTG 
GCCCGCCACG CCCTGGAGAG CAGCTTTGTG GGCTGGGGCC TGCCAGTCCA GAGCCCTCAA 
GCTCTTGTGG CCATGGAGAA GGAGGAAAAA GAGAGTCCCT TCTCCAGTGA AGAGGAAGAA 
GAAGATGTGC CTTTGGACTC AGATGTGGAA CAGGCCCTGC AGACCTTTGC CAAGACCTCA 
GGCACCATGA ATAACTACCC AACATGGCGT CGGACTCTGC TGCGCCGTGC GAAGGAGGAG 
GAGATGAAGA GGTTCTGCAA GGCCCAGACC ATCCAACGGC GACTAAATGA GATTGAGGCT 
GCCTTGAGGG AGCTAGAGGC CGAGGGCGTG AAGCTGGAGC TGGCCTTGAG GCGCCAGAGC 
AGTTCCCCAG AACAGCAAAA GAAACTATGG GTAGGACAGC TGCTACAGCT CGTTGACAAG 
AAAAACAGCC TGGTGGCTGA GGAGGCCGAG CTCATGATCA CGGTGCAGGA ATTGAATCTG 
GAGGAGAAAC AGTGGCAGCT GGACCAGGAG CTACGAGGCT ACATGAACCG GGAAGAAAAC 
CTAAAGACAG CTGCTGATCG GCAGGCTGAG GACCAGGTCC TGAGGAAGCT GGTGGATTTG 
GTCAACCAGA GAGATGCCCT CATCCGCTTC CAGGAGGAGC GCAGGCTCAG CGAGCTGGCC 
TTGGGGACAG GGGCCCAGGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073602.3
CDS	313..3516
Translation	MASPTSTNPAHAHFESFLQAQLCQDVLSSFQELCGALGLEPGGGLPQYHKIKDQLNYWSAKSLWTKLDKRAGQPVYQQGRACTSTKCLVVGAGPCGLRVAVELALLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGAKKFYGRFCTGTLDHISIRQLQLLLLKVALLLGVEIHWGVTFTGLQPPPRKGSGWRAQLQPNPPAQLANYEFDVLISAAGGKFVPEGFKVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGIDLENIVYYKDDTHYFVMTAKKQCLLRLGVLRQDWPDTNRLLGSANVVPEALQRFTRAAADFATHGKLGKLEFAQDAHGQPDVSAFDFTSMMRAESSARVQEKHGARLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMVKRWAEGAESLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPNQVRDLYDVLAKEPVQRNNDKTDTGMPATGSAGTQEELLRWCQEQTAGYPGVHVSDLSSSWADGLALCALVYRLQPGLLEPSELQGLGALEATAWALKVAENELGITPVVSAQAVVAGSDPLGLIAYLSHFHSAFKSMAHSPGPVSQASPGTSSAVLFLSKLQRTLQRSRAKENAEDAGGKKLRLEMEAETPSTEVPPDPEPGVPLTPPSQHQEAGAGDLCALCGEHLYVLERLCVNGHFFHRSCFRCHTCEATLWPGGYEQHPGDGHFYCLQHLPQTDHKAEGSDRGPESPELPTPSENSMPPGLSTPTASQEGAGPVPDPSQPTRRQIRLSSPERQRLSSLNLTPDPEMEPPPKPPRSCSALARHALESSFVGWGLPVQSPQALVAMEKEEKESPFSSEEEEEDVPLDSDVEQALQTFAKTSGTMNNYPTWRRTLLRRAKEEEMKRFCKAQTIQRRLNEIEAALRELEAEGVKLELALRRQSSSPEQQKKLWVGQLLQLVDKKNSLVAEEAELMITVQELNLEEKQWQLDQELRGYMNREENLKTAADRQAEDQVLRKLVDLVNQRDALIRFQEERRLSELALGTGAQG

Target ORF information:

RefSeq Version	NM_022765.3
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022765.3

ORF Insert Sequence:

ATGGCTTCAC CTACCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CCTGCAGGCC 
CAGCTGTGCC AGGACGTGCT GAGCAGCTTC CAGGAGCTGT GTGGGGCCCT GGGGCTGGAA 
CCCGGTGGGG GGCTGCCCCA GTACCACAAG ATCAAGGACC AGCTCAACTA CTGGAGCGCC 
AAGTCACTGT GGACCAAGCT GGACAAGCGA GCAGGCCAGC CTGTCTACCA GCAGGGCCGG 
GCCTGCACCA GCACCAAGTG CCTGGTGGTG GGTGCTGGAC CTTGCGGGCT GCGGGTCGCT 
GTGGAGCTGG CGCTGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTGCGGGC ACTCGGTGCT 
AAGAAGTTCT ACGGGCGCTT CTGCACCGGC ACCCTGGACC ACATCAGCAT CAGGCAGCTC 
CAGCTGCTTC TGCTGAAGGT AGCATTGCTG CTGGGGGTGG AAATTCACTG GGGTGTCACT 
TTCACTGGCC TCCAGCCCCC TCCTAGGAAG GGGAGTGGCT GGCGTGCCCA GCTCCAACCC 
AACCCCCCTG CCCAGCTGGC CAACTATGAA TTTGACGTCC TTATCTCGGC TGCAGGAGGT 
AAATTCGTCC CTGAAGGCTT CAAAGTTCGA GAAATGCGAG GCAAACTGGC CATTGGCATC 
ACAGCCAACT TTGTGAATGG ACGCACCGTG GAGGAGACAC AGGTGCCGGA GATCAGTGGT 
GTAGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTTC TCAAAGCCAC AGGCATTGAT 
CTGGAGAACA TTGTGTACTA CAAGGACGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TGCGGCTGGG GGTGCTGCGC CAGGACTGGC CAGACACCAA TCGGCTGCTG 
GGCAGTGCCA ATGTGGTGCC CGAGGCTCTG CAGCGCTTTA CCCGGGCAGC TGCTGACTTT 
GCCACCCATG GCAAGCTCGG GAAACTAGAG TTTGCCCAGG ATGCCCATGG GCAGCCTGAT 
GTCTCTGCCT TTGACTTCAC GAGCATGATG CGGGCAGAGA GTTCTGCTCG TGTGCAAGAG 
AAGCATGGCG CCCGCCTGCT GCTGGGACTG GTGGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC ACGGGGCTTC CTGGCAGCCT TTGATGCAGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGAGTCCCTA GAGGTGTTGG CTGAGCGTGA GAGCCTGTAC 
CAGCTTCTGT CACAGACATC CCCAGAAAAC ATGCATCGCA ATGTGGCCCA GTATGGGCTG 
GACCCAGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCAG TGACCCCCAA TCAGGTACGA 
GACCTGTATG ATGTGCTAGC CAAGGAGCCT GTGCAGAGGA ACAACGACAA GACAGATACA 
GGGATGCCAG CCACCGGGTC GGCAGGCACC CAGGAGGAGC TGCTACGCTG GTGCCAGGAG 
CAGACAGCTG GGTACCCGGG AGTCCACGTC TCCGATTTGT CTTCCTCCTG GGCTGATGGG 
CTAGCTCTGT GTGCCCTGGT GTACCGGCTG CAGCCTGGCC TGCTGGAACC CTCAGAGCTG 
CAGGGGCTGG GAGCTCTGGA AGCAACTGCT TGGGCACTAA AGGTGGCAGA GAATGAGCTG 
GGCATCACAC CGGTGGTGTC TGCACAGGCC GTGGTAGCAG GGAGTGACCC ACTGGGCCTC 
ATTGCCTACC TCAGCCACTT CCACAGTGCC TTCAAGAGCA TGGCCCACAG CCCAGGCCCT 
GTCAGCCAGG CCTCCCCAGG GACCTCCAGT GCTGTATTAT TCCTTAGTAA ACTTCAGAGG 
ACCCTGCAGC GATCCCGGGC CAAGGAAAAT GCAGAGGATG CTGGTGGCAA GAAGCTGCGC 
TTGGAGATGG AGGCCGAGAC CCCAAGTACT GAGGTGCCAC CTGACCCAGA GCCTGGTGTA 
CCCCTGACAC CCCCATCCCA ACACCAGGAG GCCGGTGCTG GGGACCTGTG TGCACTTTGT 
GGGGAACACC TCTATGTCCT GGAACGCCTC TGTGTCAACG GCCATTTCTT CCACCGGAGC 
TGCTTCCGCT GCCATACCTG TGAGGCCACA CTGTGGCCAG GTGGCTACGA GCAGCACCCA 
GGAGATGGAC ATTTCTACTG CCTCCAGCAC CTGCCCCAGA CAGACCACAA AGCGGAAGGC 
AGCGATAGAG GCCCTGAGAG TCCGGAGCTC CCCACACCAA GTGAGAATAG CATGCCACCA 
GGCCTCTCAA CTCCCACAGC CTCGCAGGAG GGGGCCGGTC CTGTTCCAGA TCCCAGCCAG 
CCCACCCGTC GGCAGATCCG CCTCTCCAGC CCGGAGCGCC AGCGGTTGTC CTCCCTTAAC 
CTTACCCCTG ACCCGGAAAT GGAGCCTCCA CCCAAGCCTC CCCGCAGCTG CTCCGCCTTG 
GCCCGCCACG CCCTGGAGAG CAGCTTTGTG GGCTGGGGCC TGCCAGTCCA GAGCCCTCAA 
GCTCTTGTGG CCATGGAGAA GGAGGAAAAA GAGAGTCCCT TCTCCAGTGA AGAGGAAGAA 
GAAGATGTGC CTTTGGACTC AGATGTGGAA CAGGCCCTGC AGACCTTTGC CAAGACCTCA 
GGCACCATGA ATAACTACCC AACATGGCGT CGGACTCTGC TGCGCCGTGC GAAGGAGGAG 
GAGATGAAGA GGTTCTGCAA GGCCCAGACC ATCCAACGGC GACTAAATGA GATTGAGGCT 
GCCTTGAGGG AGCTAGAGGC CGAGGGCGTG AAGCTGGAGC TGGCCTTGAG GCGCCAGAGC 
AGTTCCCCAG AACAGCAAAA GAAACTATGG GTAGGACAGC TGCTACAGCT CGTTGACAAG 
AAAAACAGCC TGGTGGCTGA GGAGGCCGAG CTCATGATCA CGGTGCAGGA ATTGAATCTG 
GAGGAGAAAC AGTGGCAGCT GGACCAGGAG CTACGAGGCT ACATGAACCG GGAAGAAAAC 
CTAAAGACAG CTGCTGATCG GCAGGCTGAG GACCAGGTCC TGAGGAAGCT GGTGGATTTG 
GTCAACCAGA GAGATGCCCT CATCCGCTTC CAGGAGGAGC GCAGGCTCAG CGAGCTGGCC 
TTGGGGACAG GGGCCCAGGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL1 ( NM_001159291.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001152763.1 Homo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26666
Clone ID Related Accession (Same CDS sequence)	NM_001159291.1
Accession Version	NM_001159291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	[F-actin]-monooxygenase MICAL1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB048948.1, BC042144.1 and BC009972.2. Transcript Variant: This variant (2) contains an alternate 5' exon and thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, and it lacks an alternate in-frame exon in the central coding region, compared to variant 3. The encoded isoform (2, also known as MICAL-1b) is shorter at the N-terminus, compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009972.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCAC CTACCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CCTGCAGGCC 
CAGCTGTGCC AGGACGTGCT GAGCAGCTTC CAGGAGCTGT GTGGGGCCCT GGGGCTGGAA 
CCCGGTGGGG GGCTGCCCCA GTACCACAAG ATCAAGGACC AGCTCAACTA CTGGAGCGCC 
AAGTCACTGT GGACCAAGCT GGACAAGCGA GCAGGCCAGC CTGTCTACCA GCAGGGCCGG 
GCCTGCACCA GCACCAAGTG CCTGGTGGTG GGTGCTGGAC CTTGCGGGCT GCGGGTCGCT 
GTGGAGCTGG CGCTGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTGCGGGC ACTCGGTGCT 
AAGAAGTTCT ACGGGCGCTT CTGCACCGGC ACCCTGGACC ACATCAGCAT CAGGCAGCTC 
CAGCTGCTTC TGCTGAAGGT AGCATTGCTG CTGGGGGTGG AAATTCACTG GGGTGTCACT 
TTCACTGGCC TCCAGCCCCC TCCTAGGAAG GGGAGTGGCT GGCGTGCCCA GCTCCAACCC 
AACCCCCCTG CCCAGCTGGC CAACTATGAA TTTGACGTCC TTATCTCGGC TGCAGGAGGT 
AAATTCGTCC CTGAAGGCTT CAAAGTTCGA GAAATGCGAG GCAAACTGGC CATTGGCATC 
ACAGCCAACT TTGTGAATGG ACGCACCGTG GAGGAGACAC AGGTGCCGGA GATCAGTGGT 
GTAGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTTC TCAAAGCCAC AGGCATTGAT 
CTGGAGAACA TTGTGTACTA CAAGGACGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TGCGGCTGGG GGTGCTGCGC CAGCCCTTCT GGCCCCTGGG CACTGGAGTG 
GCACGGGGCT TCCTGGCAGC CTTTGATGCA GCCTGGATGG TGAAGCGGTG GGCAGAGGGC 
GCTGAGTCCC TAGAGGTGTT GGCTGAGCGT GAGAGCCTGT ACCAGCTTCT GTCACAGACA 
TCCCCAGAAA ACATGCATCG CAATGTGGCC CAGTATGGGC TGGACCCAGC CACCCGCTAC 
CCCAACCTGA ACCTCCGGGC AGTGACCCCC AATCAGGTAC GAGACCTGTA TGATGTGCTA 
GCCAAGGAGC CTGTGCAGAG GAACAACGAC AAGACAGATA CAGGGATGCC AGCCACCGGG 
TCGGCAGGCA CCCAGGAGGA GCTGCTACGC TGGTGCCAGG AGCAGACAGC TGGGTACCCG 
GGAGTCCACG TCTCCGATTT GTCTTCCTCC TGGGCTGATG GGCTAGCTCT GTGTGCCCTG 
GTGTACCGGC TGCAGCCTGG CCTGCTGGAA CCCTCAGAGC TGCAGGGGCT GGGAGCTCTG 
GAAGCAACTG CTTGGGCACT AAAGGTGGCA GAGAATGAGC TGGGCATCAC ACCGGTGGTG 
TCTGCACAGG CCGTGGTAGC AGGGAGTGAC CCACTGGGCC TCATTGCCTA CCTCAGCCAC 
TTCCACAGTG CCTTCAAGAG CATGGCCCAC AGCCCAGGCC CTGTCAGCCA GGCCTCCCCA 
GGGACCTCCA GTGCTGTATT ATTCCTTAGT AAACTTCAGA GGACCCTGCA GCGATCCCGG 
GCCAAGGAAA ATGCAGAGGA TGCTGGTGGC AAGAAGCTGC GCTTGGAGAT GGAGGCCGAG 
ACCCCAAGTA CTGAGGTGCC ACCTGACCCA GAGCCTGGTG TACCCCTGAC ACCCCCATCC 
CAACACCAGG AGGCCGGTGC TGGGGACCTG TGTGCACTTT GTGGGGAACA CCTCTATGTC 
CTGGAACGCC TCTGTGTCAA CGGCCATTTC TTCCACCGGA GCTGCTTCCG CTGCCATACC 
TGTGAGGCCA CACTGTGGCC AGGTGGCTAC GAGCAGCACC CAGGAGATGG ACATTTCTAC 
TGCCTCCAGC ACCTGCCCCA GACAGACCAC AAAGCGGAAG GCAGCGATAG AGGCCCTGAG 
AGTCCGGAGC TCCCCACACC AAGTGAGAAT AGCATGCCAC CAGGCCTCTC AACTCCCACA 
GCCTCGCAGG AGGGGGCCGG TCCTGTTCCA GATCCCAGCC AGCCCACCCG TCGGCAGATC 
CGCCTCTCCA GCCCGGAGCG CCAGCGGTTG TCCTCCCTTA ACCTTACCCC TGACCCGGAA 
ATGGAGCCTC CACCCAAGCC TCCCCGCAGC TGCTCCGCCT TGGCCCGCCA CGCCCTGGAG 
AGCAGCTTTG TGGGCTGGGG CCTGCCAGTC CAGAGCCCTC AAGCTCTTGT GGCCATGGAG 
AAGGAGGAAA AAGAGAGTCC CTTCTCCAGT GAAGAGGAAG AAGAAGATGT GCCTTTGGAC 
TCAGATGTGG AACAGGCCCT GCAGACCTTT GCCAAGACCT CAGGCACCAT GAATAACTAC 
CCAACATGGC GTCGGACTCT GCTGCGCCGT GCGAAGGAGG AGGAGATGAA GAGGTTCTGC 
AAGGCCCAGA CCATCCAACG GCGACTAAAT GAGATTGAGG CTGCCTTGAG GGAGCTAGAG 
GCCGAGGGCG TGAAGCTGGA GCTGGCCTTG AGGCGCCAGA GCAGTTCCCC AGAACAGCAA 
AAGAAACTAT GGGTAGGACA GCTGCTACAG CTCGTTGACA AGAAAAACAG CCTGGTGGCT 
GAGGAGGCCG AGCTCATGAT CACGGTGCAG GAATTGAATC TGGAGGAGAA ACAGTGGCAG 
CTGGACCAGG AGCTACGAGG CTACATGAAC CGGGAAGAAA ACCTAAAGAC AGCTGCTGAT 
CGGCAGGCTG AGGACCAGGT CCTGAGGAAG CTGGTGGATT TGGTCAACCA GAGAGATGCC 
CTCATCCGCT TCCAGGAGGA GCGCAGGCTC AGCGAGCTGG CCTTGGGGAC AGGGGCCCAG 
GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152763.1
CDS	313..3258
Translation	MASPTSTNPAHAHFESFLQAQLCQDVLSSFQELCGALGLEPGGGLPQYHKIKDQLNYWSAKSLWTKLDKRAGQPVYQQGRACTSTKCLVVGAGPCGLRVAVELALLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGAKKFYGRFCTGTLDHISIRQLQLLLLKVALLLGVEIHWGVTFTGLQPPPRKGSGWRAQLQPNPPAQLANYEFDVLISAAGGKFVPEGFKVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGIDLENIVYYKDDTHYFVMTAKKQCLLRLGVLRQPFWPLGTGVARGFLAAFDAAWMVKRWAEGAESLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPNQVRDLYDVLAKEPVQRNNDKTDTGMPATGSAGTQEELLRWCQEQTAGYPGVHVSDLSSSWADGLALCALVYRLQPGLLEPSELQGLGALEATAWALKVAENELGITPVVSAQAVVAGSDPLGLIAYLSHFHSAFKSMAHSPGPVSQASPGTSSAVLFLSKLQRTLQRSRAKENAEDAGGKKLRLEMEAETPSTEVPPDPEPGVPLTPPSQHQEAGAGDLCALCGEHLYVLERLCVNGHFFHRSCFRCHTCEATLWPGGYEQHPGDGHFYCLQHLPQTDHKAEGSDRGPESPELPTPSENSMPPGLSTPTASQEGAGPVPDPSQPTRRQIRLSSPERQRLSSLNLTPDPEMEPPPKPPRSCSALARHALESSFVGWGLPVQSPQALVAMEKEEKESPFSSEEEEEDVPLDSDVEQALQTFAKTSGTMNNYPTWRRTLLRRAKEEEMKRFCKAQTIQRRLNEIEAALRELEAEGVKLELALRRQSSSPEQQKKLWVGQLLQLVDKKNSLVAEEAELMITVQELNLEEKQWQLDQELRGYMNREENLKTAADRQAEDQVLRKLVDLVNQRDALIRFQEERRLSELALGTGAQG

Target ORF information:

RefSeq Version	NM_001159291.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159291.1

ORF Insert Sequence:

ATGGCTTCAC CTACCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CCTGCAGGCC 
CAGCTGTGCC AGGACGTGCT GAGCAGCTTC CAGGAGCTGT GTGGGGCCCT GGGGCTGGAA 
CCCGGTGGGG GGCTGCCCCA GTACCACAAG ATCAAGGACC AGCTCAACTA CTGGAGCGCC 
AAGTCACTGT GGACCAAGCT GGACAAGCGA GCAGGCCAGC CTGTCTACCA GCAGGGCCGG 
GCCTGCACCA GCACCAAGTG CCTGGTGGTG GGTGCTGGAC CTTGCGGGCT GCGGGTCGCT 
GTGGAGCTGG CGCTGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTGCGGGC ACTCGGTGCT 
AAGAAGTTCT ACGGGCGCTT CTGCACCGGC ACCCTGGACC ACATCAGCAT CAGGCAGCTC 
CAGCTGCTTC TGCTGAAGGT AGCATTGCTG CTGGGGGTGG AAATTCACTG GGGTGTCACT 
TTCACTGGCC TCCAGCCCCC TCCTAGGAAG GGGAGTGGCT GGCGTGCCCA GCTCCAACCC 
AACCCCCCTG CCCAGCTGGC CAACTATGAA TTTGACGTCC TTATCTCGGC TGCAGGAGGT 
AAATTCGTCC CTGAAGGCTT CAAAGTTCGA GAAATGCGAG GCAAACTGGC CATTGGCATC 
ACAGCCAACT TTGTGAATGG ACGCACCGTG GAGGAGACAC AGGTGCCGGA GATCAGTGGT 
GTAGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTTC TCAAAGCCAC AGGCATTGAT 
CTGGAGAACA TTGTGTACTA CAAGGACGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TGCGGCTGGG GGTGCTGCGC CAGCCCTTCT GGCCCCTGGG CACTGGAGTG 
GCACGGGGCT TCCTGGCAGC CTTTGATGCA GCCTGGATGG TGAAGCGGTG GGCAGAGGGC 
GCTGAGTCCC TAGAGGTGTT GGCTGAGCGT GAGAGCCTGT ACCAGCTTCT GTCACAGACA 
TCCCCAGAAA ACATGCATCG CAATGTGGCC CAGTATGGGC TGGACCCAGC CACCCGCTAC 
CCCAACCTGA ACCTCCGGGC AGTGACCCCC AATCAGGTAC GAGACCTGTA TGATGTGCTA 
GCCAAGGAGC CTGTGCAGAG GAACAACGAC AAGACAGATA CAGGGATGCC AGCCACCGGG 
TCGGCAGGCA CCCAGGAGGA GCTGCTACGC TGGTGCCAGG AGCAGACAGC TGGGTACCCG 
GGAGTCCACG TCTCCGATTT GTCTTCCTCC TGGGCTGATG GGCTAGCTCT GTGTGCCCTG 
GTGTACCGGC TGCAGCCTGG CCTGCTGGAA CCCTCAGAGC TGCAGGGGCT GGGAGCTCTG 
GAAGCAACTG CTTGGGCACT AAAGGTGGCA GAGAATGAGC TGGGCATCAC ACCGGTGGTG 
TCTGCACAGG CCGTGGTAGC AGGGAGTGAC CCACTGGGCC TCATTGCCTA CCTCAGCCAC 
TTCCACAGTG CCTTCAAGAG CATGGCCCAC AGCCCAGGCC CTGTCAGCCA GGCCTCCCCA 
GGGACCTCCA GTGCTGTATT ATTCCTTAGT AAACTTCAGA GGACCCTGCA GCGATCCCGG 
GCCAAGGAAA ATGCAGAGGA TGCTGGTGGC AAGAAGCTGC GCTTGGAGAT GGAGGCCGAG 
ACCCCAAGTA CTGAGGTGCC ACCTGACCCA GAGCCTGGTG TACCCCTGAC ACCCCCATCC 
CAACACCAGG AGGCCGGTGC TGGGGACCTG TGTGCACTTT GTGGGGAACA CCTCTATGTC 
CTGGAACGCC TCTGTGTCAA CGGCCATTTC TTCCACCGGA GCTGCTTCCG CTGCCATACC 
TGTGAGGCCA CACTGTGGCC AGGTGGCTAC GAGCAGCACC CAGGAGATGG ACATTTCTAC 
TGCCTCCAGC ACCTGCCCCA GACAGACCAC AAAGCGGAAG GCAGCGATAG AGGCCCTGAG 
AGTCCGGAGC TCCCCACACC AAGTGAGAAT AGCATGCCAC CAGGCCTCTC AACTCCCACA 
GCCTCGCAGG AGGGGGCCGG TCCTGTTCCA GATCCCAGCC AGCCCACCCG TCGGCAGATC 
CGCCTCTCCA GCCCGGAGCG CCAGCGGTTG TCCTCCCTTA ACCTTACCCC TGACCCGGAA 
ATGGAGCCTC CACCCAAGCC TCCCCGCAGC TGCTCCGCCT TGGCCCGCCA CGCCCTGGAG 
AGCAGCTTTG TGGGCTGGGG CCTGCCAGTC CAGAGCCCTC AAGCTCTTGT GGCCATGGAG 
AAGGAGGAAA AAGAGAGTCC CTTCTCCAGT GAAGAGGAAG AAGAAGATGT GCCTTTGGAC 
TCAGATGTGG AACAGGCCCT GCAGACCTTT GCCAAGACCT CAGGCACCAT GAATAACTAC 
CCAACATGGC GTCGGACTCT GCTGCGCCGT GCGAAGGAGG AGGAGATGAA GAGGTTCTGC 
AAGGCCCAGA CCATCCAACG GCGACTAAAT GAGATTGAGG CTGCCTTGAG GGAGCTAGAG 
GCCGAGGGCG TGAAGCTGGA GCTGGCCTTG AGGCGCCAGA GCAGTTCCCC AGAACAGCAA 
AAGAAACTAT GGGTAGGACA GCTGCTACAG CTCGTTGACA AGAAAAACAG CCTGGTGGCT 
GAGGAGGCCG AGCTCATGAT CACGGTGCAG GAATTGAATC TGGAGGAGAA ACAGTGGCAG 
CTGGACCAGG AGCTACGAGG CTACATGAAC CGGGAAGAAA ACCTAAAGAC AGCTGCTGAT 
CGGCAGGCTG AGGACCAGGT CCTGAGGAAG CTGGTGGATT TGGTCAACCA GAGAGATGCC 
CTCATCCGCT TCCAGGAGGA GCGCAGGCTC AGCGAGCTGG CCTTGGGGAC AGGGGCCCAG 
GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL1 ( NM_001286613.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273542.1 Homo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12214
Clone ID Related Accession (Same CDS sequence)	NM_001286613.1
Accession Version	NM_001286613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	[F-actin]-monooxygenase MICAL1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK296284.1, AL109947.19 and BC042144.1. This sequence is a reference standard in the RefSeqGene project. On Nov 8, 2013 this sequence version replaced XM_005267098.1. Transcript Variant: This variant (3) represents the longest transrcript and encodes the longest isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTGTC TAAGCCACTC ATCTCTGCCC AGCTGCTGCC CTCCCCAGGA GGCCTCCATG 
GCTTCACCTA CCTCCACCAA CCCAGCGCAT GCCCACTTTG AGAGCTTCCT GCAGGCCCAG 
CTGTGCCAGG ACGTGCTGAG CAGCTTCCAG GAGCTGTGTG GGGCCCTGGG GCTGGAACCC 
GGTGGGGGGC TGCCCCAGTA CCACAAGATC AAGGACCAGC TCAACTACTG GAGCGCCAAG 
TCACTGTGGA CCAAGCTGGA CAAGCGAGCA GGCCAGCCTG TCTACCAGCA GGGCCGGGCC 
TGCACCAGCA CCAAGTGCCT GGTGGTGGGT GCTGGACCTT GCGGGCTGCG GGTCGCTGTG 
GAGCTGGCGC TGCTGGGGGC CCGAGTGGTG CTGGTGGAAA AGCGCACCAA GTTCTCTCGC 
CACAACGTGC TCCACCTCTG GCCCTTCACC ATCCACGACC TGCGGGCACT CGGTGCTAAG 
AAGTTCTACG GGCGCTTCTG CACCGGCACC CTGGACCACA TCAGCATCAG GCAGCTCCAG 
CTGCTTCTGC TGAAGGTAGC ATTGCTGCTG GGGGTGGAAA TTCACTGGGG TGTCACTTTC 
ACTGGCCTCC AGCCCCCTCC TAGGAAGGGG AGTGGCTGGC GTGCCCAGCT CCAACCCAAC 
CCCCCTGCCC AGCTGGCCAA CTATGAATTT GACGTCCTTA TCTCGGCTGC AGGAGGTAAA 
TTCGTCCCTG AAGGCTTCAA AGTTCGAGAA ATGCGAGGCA AACTGGCCAT TGGCATCACA 
GCCAACTTTG TGAATGGACG CACCGTGGAG GAGACACAGG TGCCGGAGAT CAGTGGTGTA 
GCCAGGATCT ACAACCAGAG CTTCTTCCAG AGCCTTCTCA AAGCCACAGG CATTGATCTG 
GAGAACATTG TGTACTACAA GGACGACACC CACTACTTTG TGATGACAGC CAAGAAGCAG 
TGCCTGCTGC GGCTGGGGGT GCTGCGCCAG GACTGGCCAG ACACCAATCG GCTGCTGGGC 
AGTGCCAATG TGGTGCCCGA GGCTCTGCAG CGCTTTACCC GGGCAGCTGC TGACTTTGCC 
ACCCATGGCA AGCTCGGGAA ACTAGAGTTT GCCCAGGATG CCCATGGGCA GCCTGATGTC 
TCTGCCTTTG ACTTCACGAG CATGATGCGG GCAGAGAGTT CTGCTCGTGT GCAAGAGAAG 
CATGGCGCCC GCCTGCTGCT GGGACTGGTG GGGGACTGCC TGGTGGAGCC CTTCTGGCCC 
CTGGGCACTG GAGTGGCACG GGGCTTCCTG GCAGCCTTTG ATGCAGCCTG GATGGTGAAG 
CGGTGGGCAG AGGGCGCTGA GTCCCTAGAG GTGTTGGCTG AGCGTGAGAG CCTGTACCAG 
CTTCTGTCAC AGACATCCCC AGAAAACATG CATCGCAATG TGGCCCAGTA TGGGCTGGAC 
CCAGCCACCC GCTACCCCAA CCTGAACCTC CGGGCAGTGA CCCCCAATCA GGTACGAGAC 
CTGTATGATG TGCTAGCCAA GGAGCCTGTG CAGAGGAACA ACGACAAGAC AGATACAGGG 
ATGCCAGCCA CCGGGTCGGC AGGCACCCAG GAGGAGCTGC TACGCTGGTG CCAGGAGCAG 
ACAGCTGGGT ACCCGGGAGT CCACGTCTCC GATTTGTCTT CCTCCTGGGC TGATGGGCTA 
GCTCTGTGTG CCCTGGTGTA CCGGCTGCAG CCTGGCCTGC TGGAACCCTC AGAGCTGCAG 
GGGCTGGGAG CTCTGGAAGC AACTGCTTGG GCACTAAAGG TGGCAGAGAA TGAGCTGGGC 
ATCACACCGG TGGTGTCTGC ACAGGCCGTG GTAGCAGGGA GTGACCCACT GGGCCTCATT 
GCCTACCTCA GCCACTTCCA CAGTGCCTTC AAGAGCATGG CCCACAGCCC AGGCCCTGTC 
AGCCAGGCCT CCCCAGGGAC CTCCAGTGCT GTATTATTCC TTAGTAAACT TCAGAGGACC 
CTGCAGCGAT CCCGGGCCAA GGAAAATGCA GAGGATGCTG GTGGCAAGAA GCTGCGCTTG 
GAGATGGAGG CCGAGACCCC AAGTACTGAG GTGCCACCTG ACCCAGAGCC TGGTGTACCC 
CTGACACCCC CATCCCAACA CCAGGAGGCC GGTGCTGGGG ACCTGTGTGC ACTTTGTGGG 
GAACACCTCT ATGTCCTGGA ACGCCTCTGT GTCAACGGCC ATTTCTTCCA CCGGAGCTGC 
TTCCGCTGCC ATACCTGTGA GGCCACACTG TGGCCAGGTG GCTACGAGCA GCACCCAGGA 
GATGGACATT TCTACTGCCT CCAGCACCTG CCCCAGACAG ACCACAAAGC GGAAGGCAGC 
GATAGAGGCC CTGAGAGTCC GGAGCTCCCC ACACCAAGTG AGAATAGCAT GCCACCAGGC 
CTCTCAACTC CCACAGCCTC GCAGGAGGGG GCCGGTCCTG TTCCAGATCC CAGCCAGCCC 
ACCCGTCGGC AGATCCGCCT CTCCAGCCCG GAGCGCCAGC GGTTGTCCTC CCTTAACCTT 
ACCCCTGACC CGGAAATGGA GCCTCCACCC AAGCCTCCCC GCAGCTGCTC CGCCTTGGCC 
CGCCACGCCC TGGAGAGCAG CTTTGTGGGC TGGGGCCTGC CAGTCCAGAG CCCTCAAGCT 
CTTGTGGCCA TGGAGAAGGA GGAAAAAGAG AGTCCCTTCT CCAGTGAAGA GGAAGAAGAA 
GATGTGCCTT TGGACTCAGA TGTGGAACAG GCCCTGCAGA CCTTTGCCAA GACCTCAGGC 
ACCATGAATA ACTACCCAAC ATGGCGTCGG ACTCTGCTGC GCCGTGCGAA GGAGGAGGAG 
ATGAAGAGGT TCTGCAAGGC CCAGACCATC CAACGGCGAC TAAATGAGAT TGAGGCTGCC 
TTGAGGGAGC TAGAGGCCGA GGGCGTGAAG CTGGAGCTGG CCTTGAGGCG CCAGAGCAGT 
TCCCCAGAAC AGCAAAAGAA ACTATGGGTA GGACAGCTGC TACAGCTCGT TGACAAGAAA 
AACAGCCTGG TGGCTGAGGA GGCCGAGCTC ATGATCACGG TGCAGGAATT GAATCTGGAG 
GAGAAACAGT GGCAGCTGGA CCAGGAGCTA CGAGGCTACA TGAACCGGGA AGAAAACCTA 
AAGACAGCTG CTGATCGGCA GGCTGAGGAC CAGGTCCTGA GGAAGCTGGT GGATTTGGTC 
AACCAGAGAG ATGCCCTCAT CCGCTTCCAG GAGGAGCGCA GGCTCAGCGA GCTGGCCTTG 
GGGACAGGGG CCCAGGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273542.1
CDS	292..3552
Translation	MSCLSHSSLPSCCPPQEASMASPTSTNPAHAHFESFLQAQLCQDVLSSFQELCGALGLEPGGGLPQYHKIKDQLNYWSAKSLWTKLDKRAGQPVYQQGRACTSTKCLVVGAGPCGLRVAVELALLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGAKKFYGRFCTGTLDHISIRQLQLLLLKVALLLGVEIHWGVTFTGLQPPPRKGSGWRAQLQPNPPAQLANYEFDVLISAAGGKFVPEGFKVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGIDLENIVYYKDDTHYFVMTAKKQCLLRLGVLRQDWPDTNRLLGSANVVPEALQRFTRAAADFATHGKLGKLEFAQDAHGQPDVSAFDFTSMMRAESSARVQEKHGARLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMVKRWAEGAESLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPNQVRDLYDVLAKEPVQRNNDKTDTGMPATGSAGTQEELLRWCQEQTAGYPGVHVSDLSSSWADGLALCALVYRLQPGLLEPSELQGLGALEATAWALKVAENELGITPVVSAQAVVAGSDPLGLIAYLSHFHSAFKSMAHSPGPVSQASPGTSSAVLFLSKLQRTLQRSRAKENAEDAGGKKLRLEMEAETPSTEVPPDPEPGVPLTPPSQHQEAGAGDLCALCGEHLYVLERLCVNGHFFHRSCFRCHTCEATLWPGGYEQHPGDGHFYCLQHLPQTDHKAEGSDRGPESPELPTPSENSMPPGLSTPTASQEGAGPVPDPSQPTRRQIRLSSPERQRLSSLNLTPDPEMEPPPKPPRSCSALARHALESSFVGWGLPVQSPQALVAMEKEEKESPFSSEEEEEDVPLDSDVEQALQTFAKTSGTMNNYPTWRRTLLRRAKEEEMKRFCKAQTIQRRLNEIEAALRELEAEGVKLELALRRQSSSPEQQKKLWVGQLLQLVDKKNSLVAEEAELMITVQELNLEEKQWQLDQELRGYMNREENLKTAADRQAEDQVLRKLVDLVNQRDALIRFQEERRLSELALGTGAQG

Target ORF information:

RefSeq Version	NM_001286613.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286613.1

ORF Insert Sequence:

ATGTCTTGTC TAAGCCACTC ATCTCTGCCC AGCTGCTGCC CTCCCCAGGA GGCCTCCATG 
GCTTCACCTA CCTCCACCAA CCCAGCGCAT GCCCACTTTG AGAGCTTCCT GCAGGCCCAG 
CTGTGCCAGG ACGTGCTGAG CAGCTTCCAG GAGCTGTGTG GGGCCCTGGG GCTGGAACCC 
GGTGGGGGGC TGCCCCAGTA CCACAAGATC AAGGACCAGC TCAACTACTG GAGCGCCAAG 
TCACTGTGGA CCAAGCTGGA CAAGCGAGCA GGCCAGCCTG TCTACCAGCA GGGCCGGGCC 
TGCACCAGCA CCAAGTGCCT GGTGGTGGGT GCTGGACCTT GCGGGCTGCG GGTCGCTGTG 
GAGCTGGCGC TGCTGGGGGC CCGAGTGGTG CTGGTGGAAA AGCGCACCAA GTTCTCTCGC 
CACAACGTGC TCCACCTCTG GCCCTTCACC ATCCACGACC TGCGGGCACT CGGTGCTAAG 
AAGTTCTACG GGCGCTTCTG CACCGGCACC CTGGACCACA TCAGCATCAG GCAGCTCCAG 
CTGCTTCTGC TGAAGGTAGC ATTGCTGCTG GGGGTGGAAA TTCACTGGGG TGTCACTTTC 
ACTGGCCTCC AGCCCCCTCC TAGGAAGGGG AGTGGCTGGC GTGCCCAGCT CCAACCCAAC 
CCCCCTGCCC AGCTGGCCAA CTATGAATTT GACGTCCTTA TCTCGGCTGC AGGAGGTAAA 
TTCGTCCCTG AAGGCTTCAA AGTTCGAGAA ATGCGAGGCA AACTGGCCAT TGGCATCACA 
GCCAACTTTG TGAATGGACG CACCGTGGAG GAGACACAGG TGCCGGAGAT CAGTGGTGTA 
GCCAGGATCT ACAACCAGAG CTTCTTCCAG AGCCTTCTCA AAGCCACAGG CATTGATCTG 
GAGAACATTG TGTACTACAA GGACGACACC CACTACTTTG TGATGACAGC CAAGAAGCAG 
TGCCTGCTGC GGCTGGGGGT GCTGCGCCAG GACTGGCCAG ACACCAATCG GCTGCTGGGC 
AGTGCCAATG TGGTGCCCGA GGCTCTGCAG CGCTTTACCC GGGCAGCTGC TGACTTTGCC 
ACCCATGGCA AGCTCGGGAA ACTAGAGTTT GCCCAGGATG CCCATGGGCA GCCTGATGTC 
TCTGCCTTTG ACTTCACGAG CATGATGCGG GCAGAGAGTT CTGCTCGTGT GCAAGAGAAG 
CATGGCGCCC GCCTGCTGCT GGGACTGGTG GGGGACTGCC TGGTGGAGCC CTTCTGGCCC 
CTGGGCACTG GAGTGGCACG GGGCTTCCTG GCAGCCTTTG ATGCAGCCTG GATGGTGAAG 
CGGTGGGCAG AGGGCGCTGA GTCCCTAGAG GTGTTGGCTG AGCGTGAGAG CCTGTACCAG 
CTTCTGTCAC AGACATCCCC AGAAAACATG CATCGCAATG TGGCCCAGTA TGGGCTGGAC 
CCAGCCACCC GCTACCCCAA CCTGAACCTC CGGGCAGTGA CCCCCAATCA GGTACGAGAC 
CTGTATGATG TGCTAGCCAA GGAGCCTGTG CAGAGGAACA ACGACAAGAC AGATACAGGG 
ATGCCAGCCA CCGGGTCGGC AGGCACCCAG GAGGAGCTGC TACGCTGGTG CCAGGAGCAG 
ACAGCTGGGT ACCCGGGAGT CCACGTCTCC GATTTGTCTT CCTCCTGGGC TGATGGGCTA 
GCTCTGTGTG CCCTGGTGTA CCGGCTGCAG CCTGGCCTGC TGGAACCCTC AGAGCTGCAG 
GGGCTGGGAG CTCTGGAAGC AACTGCTTGG GCACTAAAGG TGGCAGAGAA TGAGCTGGGC 
ATCACACCGG TGGTGTCTGC ACAGGCCGTG GTAGCAGGGA GTGACCCACT GGGCCTCATT 
GCCTACCTCA GCCACTTCCA CAGTGCCTTC AAGAGCATGG CCCACAGCCC AGGCCCTGTC 
AGCCAGGCCT CCCCAGGGAC CTCCAGTGCT GTATTATTCC TTAGTAAACT TCAGAGGACC 
CTGCAGCGAT CCCGGGCCAA GGAAAATGCA GAGGATGCTG GTGGCAAGAA GCTGCGCTTG 
GAGATGGAGG CCGAGACCCC AAGTACTGAG GTGCCACCTG ACCCAGAGCC TGGTGTACCC 
CTGACACCCC CATCCCAACA CCAGGAGGCC GGTGCTGGGG ACCTGTGTGC ACTTTGTGGG 
GAACACCTCT ATGTCCTGGA ACGCCTCTGT GTCAACGGCC ATTTCTTCCA CCGGAGCTGC 
TTCCGCTGCC ATACCTGTGA GGCCACACTG TGGCCAGGTG GCTACGAGCA GCACCCAGGA 
GATGGACATT TCTACTGCCT CCAGCACCTG CCCCAGACAG ACCACAAAGC GGAAGGCAGC 
GATAGAGGCC CTGAGAGTCC GGAGCTCCCC ACACCAAGTG AGAATAGCAT GCCACCAGGC 
CTCTCAACTC CCACAGCCTC GCAGGAGGGG GCCGGTCCTG TTCCAGATCC CAGCCAGCCC 
ACCCGTCGGC AGATCCGCCT CTCCAGCCCG GAGCGCCAGC GGTTGTCCTC CCTTAACCTT 
ACCCCTGACC CGGAAATGGA GCCTCCACCC AAGCCTCCCC GCAGCTGCTC CGCCTTGGCC 
CGCCACGCCC TGGAGAGCAG CTTTGTGGGC TGGGGCCTGC CAGTCCAGAG CCCTCAAGCT 
CTTGTGGCCA TGGAGAAGGA GGAAAAAGAG AGTCCCTTCT CCAGTGAAGA GGAAGAAGAA 
GATGTGCCTT TGGACTCAGA TGTGGAACAG GCCCTGCAGA CCTTTGCCAA GACCTCAGGC 
ACCATGAATA ACTACCCAAC ATGGCGTCGG ACTCTGCTGC GCCGTGCGAA GGAGGAGGAG 
ATGAAGAGGT TCTGCAAGGC CCAGACCATC CAACGGCGAC TAAATGAGAT TGAGGCTGCC 
TTGAGGGAGC TAGAGGCCGA GGGCGTGAAG CTGGAGCTGG CCTTGAGGCG CCAGAGCAGT 
TCCCCAGAAC AGCAAAAGAA ACTATGGGTA GGACAGCTGC TACAGCTCGT TGACAAGAAA 
AACAGCCTGG TGGCTGAGGA GGCCGAGCTC ATGATCACGG TGCAGGAATT GAATCTGGAG 
GAGAAACAGT GGCAGCTGGA CCAGGAGCTA CGAGGCTACA TGAACCGGGA AGAAAACCTA 
AAGACAGCTG CTGATCGGCA GGCTGAGGAC CAGGTCCTGA GGAAGCTGGT GGATTTGGTC 
AACCAGAGAG ATGCCCTCAT CCGCTTCCAG GAGGAGCGCA GGCTCAGCGA GCTGGCCTTG 
GGGACAGGGG CCCAGGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure-function studies of MICAL, the unusual multidomain flavoenzyme involved in actin cytoskeleton dynamics.
Archives of biochemistry and biophysics632118-141(2017 Oct)
Vanoni MA

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

MICAL1 controls cell invasive phenotype via regulating oxidative stress in breast cancer cells.
BMC cancer16489(2016 07)
Deng W,Wang Y,Gu L,Duan B,Cui J,Zhang Y,Chen Y,Sun S,Dong J,Du J

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MICAL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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