VPS16 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS16
Entrez Gene ID	64601
Full Name	VPS16, CORVET/HOPS core subunit
Synonyms	hVPS16
General protein information	Preferred Names VPS16, CORVET/HOPS core subunit Names vacuolar protein sorting-associated protein 16 homolog vacuolar protein sorting 16 homolog vacuolar protein sorting protein 16
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20p13
Summary	Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009].
Disorder MIM:	608550

mRNA and Protein(s)

mRNA	Protein	Name
NM_022575.3	NP_072097.2	vacuolar protein sorting-associated protein 16 homolog isoform 1
NM_080413.2	NP_536338.1	vacuolar protein sorting-associated protein 16 homolog isoform 3
NM_022575.4	NP_072097.2	vacuolar protein sorting-associated protein 16 homolog isoform 1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000529	XP_310557.3
Arabidopsis thaliana (thale cress)	VCL1	NP_565879.1
Rattus norvegicus (Norway rat)	Vps16	NP_001005541.1
Danio rerio (zebrafish)	vps16	NP_001091659.1
Drosophila melanogaster (fruit fly)	Vps16A	NP_649877.1
Schizosaccharomyces pombe (fission yeast)	vps16	NP_593444.1
Neurospora crassa	NCU06268	XP_962872.2
Oryza sativa (rice)	Os01g0666800	NP_001043804.2
Homo sapiens (human)	VPS16	NP_072097.2
Bos taurus (cattle)	VPS16	NP_001015522.1
Gallus gallus (chicken)	VPS16	NP_001258079.1
Magnaporthe oryzae (rice blast fungus)	MGG_05256	XP_003712813.1
Xenopus tropicalis (tropical clawed frog)	vps16	NP_001004976.1
	VPS16	XP_002798262.1
Canis lupus familiaris (dog)	VPS16	XP_003639898.1
Mus musculus (house mouse)	Vps16	NP_085036.3

Gene Ontology
Bibliography

Related articles in PubMed

Mutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms.
Kondo H, Maksimova N, Otomo T, Kato H, Imai A, Asano Y, Kobayashi K, Nojima S, Nakaya A, Hamada Y, Irahara K, Gurinova E, Sukhomyasova A, Nogovicina A, Savvina M, Yoshimori T, Ozono K, Sakai N
Human molecular genetics26(1)173-183(2017 01)

Homozygous mutation of VPS16 gene is responsible for an autosomal recessive adolescent-onset primary dystonia.
Cai X, Chen X, Wu S, Liu W, Zhang X, Zhang D, He S, Wang B, Zhang M, Zhang Y, Li Z, Luo K, Cai Z, Li W
Scientific reports625834(2016 May)

Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.
van der Kant R, Jonker CT, Wijdeven RH, Bakker J, Janssen L, Klumperman J, Neefjes J
The Journal of biological chemistry290(51)30280-90(2015 Dec)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome Fusion with Endosomes and Autophagosomes.
Wartosch L, G?nesdogan U, Graham SC, Luzio JP
Traffic (Copenhagen, Denmark)16(7)727-42(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HOPS subunit Vps16 recruits Vps33A to the human HOPS complex; residues 642-736 are necessary and sufficient for this interaction.
Title: Structural basis of Vps33A recruitment to the human HOPS complex by Vps16.

The following VPS16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00886	NM_022575.4 Latest version!	Homo sapiens VPS16 core subunit of CORVET and HOPS complexes (VPS16), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$594.30 $849.00
OHu01249	NM_080413.2 Latest version!	Homo sapiens VPS16 core subunit of CORVET and HOPS complexes (VPS16), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$489.30 $699.00
View Old Accession Versions >>
OHu00886	NM_022575.3	Homo sapiens VPS16, CORVET/HOPS core subunit (VPS16), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$594.30 $849.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS16 ( NM_022575.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_072097.2 Homo sapiens VPS16, CORVET/HOPS core subunit (VPS16), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00886
Clone ID Related Accession (Same CDS sequence)	NM_022575.3 , NM_022575.4
Accession Version	NM_022575.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 16 homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161656.20, BU845142.1, AF308801.1 and BM468030.1. On Sep 6, 2013 this sequence version replaced NM_022575.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF308801.1, SRR1803615.212019.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTGCT ACACGGCGAA CTGGAACCCA CTCGGGGACT CTGCCTTTTA CCGGAAATAT 
GAGCTGTACA GCATGGACTG GGACCTGAAG GAGGAACTCA GGGATTGCCT GGTGGCTGCT 
GCACCCTATG GGGGCCCCAT TGCACTGCTG AGGAACCCCT GGCGGAAGGA GAAAGCTGCT 
AGTGTGAGGC CAGTGCTCGA TATATACTCT GCTTCCGGCA TGCCTCTGGC CAGCCTGCTG 
TGGAAGAGTG GACCCGTGGT GTCCCTGGGC TGGTCAGCTG AGGAGGAGCT GCTCTGTGTG 
CAGGAAGATG GTGCTGTACT GGTTTATGGG CTTCATGGTG ACTTCCGGAG ACACTTCAGC 
ATGGGCAATG AAGTGCTCCA GAACCGGGTT CTGGATGCCC GGATCTTTCA CACTGAGTTT 
GGTTCCGGAG TGGCCATCCT CACAGGGGCC CACCGCTTCA CCCTCAGTGC CAATGTGGGT 
GACCTCAAAC TCCGCCGGAT GCCAGAGGTG CCAGGTCTGC AAAGTGCACC CTCCTGCTGG 
ACTGTGCTGT GCCAGGACCG AGTGGCACAC ATTCTTCTGG CTGTGGGGCC TGACCTTTAC 
CTCTTGGACC ATGCAGCCTG CTCCGCAGTG ACGCCCCCTG GCCTGGCCCC AGGAGTAAGC 
AGCTTCCTAC AGATGGCTGT CTCCTTCACC TACCGACACC TGGCACTCTT CACAGACACA 
GGCTACATCT GGATGGGGAC AGCATCACTC AAGGAGAAGC TATGTGAGTT CAACTGCAAC 
ATCCGGGCAC CTCCAAAGCA GATGGTCTGG TGCAGCCGTC CTCGTAGCAA GGAGAGGGCC 
GTGGTGGTGG CCTGGGAAAG GCGGCTGATG GTGGTGGGCG ATGCACCCGA GAGCATCCAG 
TTTGTGCTGG ATGAGGACTC CTACCTGGTG CCTGAGCTCG ATGGGGTCCG CATCTTCTCC 
CGCAGCACCC ACGAGTTCCT GCATGAGGTT CCAGCGGCCA GCGAGGAAAT CTTCAAAATT 
GCCTCAATGG CCCCCGGGGC GCTGCTCCTG GAGGCTCAGA AGGAGTATGA GAAAGAGAGC 
CAGAAGGCGG ACGAGTACCT GCGGGAGATC CAGGAGCTGG GCCAGCTGAC CCAGGCCGTG 
CAGCAGTGCA TTGAGGCTGC AGGACATGAG CACCAGCCAG ACATGCAGAA GAGTCTGCTC 
AGGGCCGCCT CCTTCGGAAA GTGTTTCCTG GACAGATTTC CACCCGACAG CTTCGTGCAC 
ATGTGTCAGG ACCTGCGTGT GCTCAATGCT GTTCGGGACT ATCACATCGG GATCCCGCTC 
ACCTATAGCC AATATAAGCA GCTCACCATC CAGGTGCTGC TGGACAGGCT CGTGTTGCGG 
AGACTTTACC CCCTGGCCAT CCAGATATGC GAGTACTTGC GCCTTCCTGA AGTACAGGGC 
GTCAGCAGGA TCCTGGCCCA CTGGGCCTGC TACAAGGTGC AACAGAAGGA TGTCTCAGAT 
GAGGATGTGG CTCGAGCCAT TAACCAGAAG CTGGGGGACA CGCCTGGTGT CTCTTACTCC 
GACATTGCTG CACGAGCCTA TGGTTGTGGC CGCACGGAGC TGGCCATCAA GCTGCTGGAG 
TATGAGCCAC GCTCAGGGGA GCAGGTACCC CTTCTCCTAA AGATGAAGAG GAGCAAACTG 
GCACTAAGCA AGGCCATCGA GAGCGGGGAC ACTGACCTGG TGTTCACGGT GTTGCTGCAC 
CTGAAGAACG AGCTGAACCG AGGAGATTTT TTCATGACCC TTCGGAATCA GCCCATGGCC 
CTCAGTTTGT ACCGACAGTT CTGTAAGCAT CAGGAGCTAG AGACGCTGAA GGACCTTTAC 
AATCAGGATG ACAATCACCA GGAATTGGGC AGCTTCCACA TCCGAGCCAG CTATGCTGCA 
GAAGAGCGTA TTGAGGGGCG AGTAGCAGCT CTGCAGACAG CCGCCGATGC CTTCTACAAG 
GCCAAGAATG AGTTTGCAGC CAAGGCTACA GAGGATCAAA TGCGGCTCCT ACGGCTGCAG 
CGGCGCCTAG AAGACGAGCT GGGGGGCCAG TTCCTAGACC TGTCTCTACA TGACACAGTT 
ACCACCCTCA TTCTTGGCGG TCACAACAAG CGTGCAGAGC AGCTGGCACG TGACTTCCGC 
ATCCCTGACA AGAGGCTCTG GTGGCTGAAG CTGACTGCCC TGGCAGATTT GGAAGATTGG 
GAAGAGCTAG AGAAGTTTTC CAAGAGCAAG AAATCACCCA TTGGCTACCT GCCTTTTGTG 
GAGATCTGCA TGAAACAACA TAACAAATAC GAAGCCAAGA AGTATGCTTC CCGCGTGGGT 
CCCGAGCAGA AGGTCAAGGC TTTGCTTCTT GTTGGCGATG TGGCTCAGGC TGCAGATGTG 
GCCATCGAAC ACCGGAATGA GGCTGAGCTG AGCCTCGTAT TGTCCCACTG CACGGGAGCC 
ACAGATGGGG CCACAGCTGA CAAGATTCAA CGGGCCAGGG CACAAGCCCA GAAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072097.2
CDS	79..2598
Translation	MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKAASVRPVLDIYSASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCWTVLCQDRVAHILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGDAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAVRDYHIGIPLTYSQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRLQRRLEDELGGQFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLTALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKYEAKKYASRVGPEQKVKALLLVGDVAQAADVAIEHRNEAELSLVLSHCTGATDGATADKIQRARAQAQKK

Target ORF information:

RefSeq Version	NM_022575.3
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS16, CORVET/HOPS core subunit (VPS16), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022575.3

ORF Insert Sequence:

ATGGACTGCT ACACGGCGAA CTGGAACCCA CTCGGGGACT CTGCCTTTTA CCGGAAATAT 
GAGCTGTACA GCATGGACTG GGACCTGAAG GAGGAACTCA GGGATTGCCT GGTGGCTGCT 
GCACCCTATG GGGGCCCCAT TGCACTGCTG AGGAACCCCT GGCGGAAGGA GAAAGCTGCT 
AGTGTGAGGC CAGTGCTCGA TATATACTCT GCTTCCGGCA TGCCTCTGGC CAGCCTGCTG 
TGGAAGAGTG GACCCGTGGT GTCCCTGGGC TGGTCAGCTG AGGAGGAGCT GCTCTGTGTG 
CAGGAAGATG GTGCTGTACT GGTTTATGGG CTTCATGGTG ACTTCCGGAG ACACTTCAGC 
ATGGGCAATG AAGTGCTCCA GAACCGGGTT CTGGATGCCC GGATCTTTCA CACTGAGTTT 
GGTTCCGGAG TGGCCATCCT CACAGGGGCC CACCGCTTCA CCCTCAGTGC CAATGTGGGT 
GACCTCAAAC TCCGCCGGAT GCCAGAGGTG CCAGGTCTGC AAAGTGCACC CTCCTGCTGG 
ACTGTGCTGT GCCAGGACCG AGTGGCACAC ATTCTTCTGG CTGTGGGGCC TGACCTTTAC 
CTCTTGGACC ATGCAGCCTG CTCCGCAGTG ACGCCCCCTG GCCTGGCCCC AGGAGTAAGC 
AGCTTCCTAC AGATGGCTGT CTCCTTCACC TACCGACACC TGGCACTCTT CACAGACACA 
GGCTACATCT GGATGGGGAC AGCATCACTC AAGGAGAAGC TATGTGAGTT CAACTGCAAC 
ATCCGGGCAC CTCCAAAGCA GATGGTCTGG TGCAGCCGTC CTCGTAGCAA GGAGAGGGCC 
GTGGTGGTGG CCTGGGAAAG GCGGCTGATG GTGGTGGGCG ATGCACCCGA GAGCATCCAG 
TTTGTGCTGG ATGAGGACTC CTACCTGGTG CCTGAGCTCG ATGGGGTCCG CATCTTCTCC 
CGCAGCACCC ACGAGTTCCT GCATGAGGTT CCAGCGGCCA GCGAGGAAAT CTTCAAAATT 
GCCTCAATGG CCCCCGGGGC GCTGCTCCTG GAGGCTCAGA AGGAGTATGA GAAAGAGAGC 
CAGAAGGCGG ACGAGTACCT GCGGGAGATC CAGGAGCTGG GCCAGCTGAC CCAGGCCGTG 
CAGCAGTGCA TTGAGGCTGC AGGACATGAG CACCAGCCAG ACATGCAGAA GAGTCTGCTC 
AGGGCCGCCT CCTTCGGAAA GTGTTTCCTG GACAGATTTC CACCCGACAG CTTCGTGCAC 
ATGTGTCAGG ACCTGCGTGT GCTCAATGCT GTTCGGGACT ATCACATCGG GATCCCGCTC 
ACCTATAGCC AATATAAGCA GCTCACCATC CAGGTGCTGC TGGACAGGCT CGTGTTGCGG 
AGACTTTACC CCCTGGCCAT CCAGATATGC GAGTACTTGC GCCTTCCTGA AGTACAGGGC 
GTCAGCAGGA TCCTGGCCCA CTGGGCCTGC TACAAGGTGC AACAGAAGGA TGTCTCAGAT 
GAGGATGTGG CTCGAGCCAT TAACCAGAAG CTGGGGGACA CGCCTGGTGT CTCTTACTCC 
GACATTGCTG CACGAGCCTA TGGTTGTGGC CGCACGGAGC TGGCCATCAA GCTGCTGGAG 
TATGAGCCAC GCTCAGGGGA GCAGGTACCC CTTCTCCTAA AGATGAAGAG GAGCAAACTG 
GCACTAAGCA AGGCCATCGA GAGCGGGGAC ACTGACCTGG TGTTCACGGT GTTGCTGCAC 
CTGAAGAACG AGCTGAACCG AGGAGATTTT TTCATGACCC TTCGGAATCA GCCCATGGCC 
CTCAGTTTGT ACCGACAGTT CTGTAAGCAT CAGGAGCTAG AGACGCTGAA GGACCTTTAC 
AATCAGGATG ACAATCACCA GGAATTGGGC AGCTTCCACA TCCGAGCCAG CTATGCTGCA 
GAAGAGCGTA TTGAGGGGCG AGTAGCAGCT CTGCAGACAG CCGCCGATGC CTTCTACAAG 
GCCAAGAATG AGTTTGCAGC CAAGGCTACA GAGGATCAAA TGCGGCTCCT ACGGCTGCAG 
CGGCGCCTAG AAGACGAGCT GGGGGGCCAG TTCCTAGACC TGTCTCTACA TGACACAGTT 
ACCACCCTCA TTCTTGGCGG TCACAACAAG CGTGCAGAGC AGCTGGCACG TGACTTCCGC 
ATCCCTGACA AGAGGCTCTG GTGGCTGAAG CTGACTGCCC TGGCAGATTT GGAAGATTGG 
GAAGAGCTAG AGAAGTTTTC CAAGAGCAAG AAATCACCCA TTGGCTACCT GCCTTTTGTG 
GAGATCTGCA TGAAACAACA TAACAAATAC GAAGCCAAGA AGTATGCTTC CCGCGTGGGT 
CCCGAGCAGA AGGTCAAGGC TTTGCTTCTT GTTGGCGATG TGGCTCAGGC TGCAGATGTG 
GCCATCGAAC ACCGGAATGA GGCTGAGCTG AGCCTCGTAT TGTCCCACTG CACGGGAGCC 
ACAGATGGGG CCACAGCTGA CAAGATTCAA CGGGCCAGGG CACAAGCCCA GAAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS16 ( NM_022575.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_072097.2 Homo sapiens VPS16 core subunit of CORVET and HOPS complexes (VPS16), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00886
Clone ID Related Accession (Same CDS sequence)	NM_022575.3 , NM_022575.4
Accession Version	NM_022575.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 16 homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU845142.1, AF308801.1 and BM468030.1. On Nov 23, 2018 this sequence version replaced NM_022575.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.940372.1, SRR1163655.61091.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000380445.8/ ENSP00000369810.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACTGCT ACACGGCGAA CTGGAACCCA CTCGGGGACT CTGCCTTTTA CCGGAAATAT 
GAGCTGTACA GCATGGACTG GGACCTGAAG GAGGAACTCA GGGATTGCCT GGTGGCTGCT 
GCACCCTATG GGGGCCCCAT TGCACTGCTG AGGAACCCCT GGCGGAAGGA GAAAGCTGCT 
AGTGTGAGGC CAGTGCTCGA TATATACTCT GCTTCCGGCA TGCCTCTGGC CAGCCTGCTG 
TGGAAGAGTG GACCCGTGGT GTCCCTGGGC TGGTCAGCTG AGGAGGAGCT GCTCTGTGTG 
CAGGAAGATG GTGCTGTACT GGTTTATGGG CTTCATGGTG ACTTCCGGAG ACACTTCAGC 
ATGGGCAATG AAGTGCTCCA GAACCGGGTT CTGGATGCCC GGATCTTTCA CACTGAGTTT 
GGTTCCGGAG TGGCCATCCT CACAGGGGCC CACCGCTTCA CCCTCAGTGC CAATGTGGGT 
GACCTCAAAC TCCGCCGGAT GCCAGAGGTG CCAGGTCTGC AAAGTGCACC CTCCTGCTGG 
ACTGTGCTGT GCCAGGACCG AGTGGCACAC ATTCTTCTGG CTGTGGGGCC TGACCTTTAC 
CTCTTGGACC ATGCAGCCTG CTCCGCAGTG ACGCCCCCTG GCCTGGCCCC AGGAGTAAGC 
AGCTTCCTAC AGATGGCTGT CTCCTTCACC TACCGACACC TGGCACTCTT CACAGACACA 
GGCTACATCT GGATGGGGAC AGCATCACTC AAGGAGAAGC TATGTGAGTT CAACTGCAAC 
ATCCGGGCAC CTCCAAAGCA GATGGTCTGG TGCAGCCGTC CTCGTAGCAA GGAGAGGGCC 
GTGGTGGTGG CCTGGGAAAG GCGGCTGATG GTGGTGGGCG ATGCACCCGA GAGCATCCAG 
TTTGTGCTGG ATGAGGACTC CTACCTGGTG CCTGAGCTCG ATGGGGTCCG CATCTTCTCC 
CGCAGCACCC ACGAGTTCCT GCATGAGGTT CCAGCGGCCA GCGAGGAAAT CTTCAAAATT 
GCCTCAATGG CCCCCGGGGC GCTGCTCCTG GAGGCTCAGA AGGAGTATGA GAAAGAGAGC 
CAGAAGGCGG ACGAGTACCT GCGGGAGATC CAGGAGCTGG GCCAGCTGAC CCAGGCCGTG 
CAGCAGTGCA TTGAGGCTGC AGGACATGAG CACCAGCCAG ACATGCAGAA GAGTCTGCTC 
AGGGCCGCCT CCTTCGGAAA GTGTTTCCTG GACAGATTTC CACCCGACAG CTTCGTGCAC 
ATGTGTCAGG ACCTGCGTGT GCTCAATGCT GTTCGGGACT ATCACATCGG GATCCCGCTC 
ACCTATAGCC AATATAAGCA GCTCACCATC CAGGTGCTGC TGGACAGGCT CGTGTTGCGG 
AGACTTTACC CCCTGGCCAT CCAGATATGC GAGTACTTGC GCCTTCCTGA AGTACAGGGC 
GTCAGCAGGA TCCTGGCCCA CTGGGCCTGC TACAAGGTGC AACAGAAGGA TGTCTCAGAT 
GAGGATGTGG CTCGAGCCAT TAACCAGAAG CTGGGGGACA CGCCTGGTGT CTCTTACTCC 
GACATTGCTG CACGAGCCTA TGGTTGTGGC CGCACGGAGC TGGCCATCAA GCTGCTGGAG 
TATGAGCCAC GCTCAGGGGA GCAGGTACCC CTTCTCCTAA AGATGAAGAG GAGCAAACTG 
GCACTAAGCA AGGCCATCGA GAGCGGGGAC ACTGACCTGG TGTTCACGGT GTTGCTGCAC 
CTGAAGAACG AGCTGAACCG AGGAGATTTT TTCATGACCC TTCGGAATCA GCCCATGGCC 
CTCAGTTTGT ACCGACAGTT CTGTAAGCAT CAGGAGCTAG AGACGCTGAA GGACCTTTAC 
AATCAGGATG ACAATCACCA GGAATTGGGC AGCTTCCACA TCCGAGCCAG CTATGCTGCA 
GAAGAGCGTA TTGAGGGGCG AGTAGCAGCT CTGCAGACAG CCGCCGATGC CTTCTACAAG 
GCCAAGAATG AGTTTGCAGC CAAGGCTACA GAGGATCAAA TGCGGCTCCT ACGGCTGCAG 
CGGCGCCTAG AAGACGAGCT GGGGGGCCAG TTCCTAGACC TGTCTCTACA TGACACAGTT 
ACCACCCTCA TTCTTGGCGG TCACAACAAG CGTGCAGAGC AGCTGGCACG TGACTTCCGC 
ATCCCTGACA AGAGGCTCTG GTGGCTGAAG CTGACTGCCC TGGCAGATTT GGAAGATTGG 
GAAGAGCTAG AGAAGTTTTC CAAGAGCAAG AAATCACCCA TTGGCTACCT GCCTTTTGTG 
GAGATCTGCA TGAAACAACA TAACAAATAC GAAGCCAAGA AGTATGCTTC CCGCGTGGGT 
CCCGAGCAGA AGGTCAAGGC TTTGCTTCTT GTTGGCGATG TGGCTCAGGC TGCAGATGTG 
GCCATCGAAC ACCGGAATGA GGCTGAGCTG AGCCTCGTAT TGTCCCACTG CACGGGAGCC 
ACAGATGGGG CCACAGCTGA CAAGATTCAA CGGGCCAGGG CACAAGCCCA GAAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072097.2
CDS	31..2550
Translation	MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKAASVRPVLDIYSASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCWTVLCQDRVAHILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGDAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAVRDYHIGIPLTYSQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRLQRRLEDELGGQFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLTALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKYEAKKYASRVGPEQKVKALLLVGDVAQAADVAIEHRNEAELSLVLSHCTGATDGATADKIQRARAQAQKK

Target ORF information:

RefSeq Version	NM_022575.4
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS16 core subunit of CORVET and HOPS complexes (VPS16), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022575.4

ORF Insert Sequence:

ATGGACTGCT ACACGGCGAA CTGGAACCCA CTCGGGGACT CTGCCTTTTA CCGGAAATAT 
GAGCTGTACA GCATGGACTG GGACCTGAAG GAGGAACTCA GGGATTGCCT GGTGGCTGCT 
GCACCCTATG GGGGCCCCAT TGCACTGCTG AGGAACCCCT GGCGGAAGGA GAAAGCTGCT 
AGTGTGAGGC CAGTGCTCGA TATATACTCT GCTTCCGGCA TGCCTCTGGC CAGCCTGCTG 
TGGAAGAGTG GACCCGTGGT GTCCCTGGGC TGGTCAGCTG AGGAGGAGCT GCTCTGTGTG 
CAGGAAGATG GTGCTGTACT GGTTTATGGG CTTCATGGTG ACTTCCGGAG ACACTTCAGC 
ATGGGCAATG AAGTGCTCCA GAACCGGGTT CTGGATGCCC GGATCTTTCA CACTGAGTTT 
GGTTCCGGAG TGGCCATCCT CACAGGGGCC CACCGCTTCA CCCTCAGTGC CAATGTGGGT 
GACCTCAAAC TCCGCCGGAT GCCAGAGGTG CCAGGTCTGC AAAGTGCACC CTCCTGCTGG 
ACTGTGCTGT GCCAGGACCG AGTGGCACAC ATTCTTCTGG CTGTGGGGCC TGACCTTTAC 
CTCTTGGACC ATGCAGCCTG CTCCGCAGTG ACGCCCCCTG GCCTGGCCCC AGGAGTAAGC 
AGCTTCCTAC AGATGGCTGT CTCCTTCACC TACCGACACC TGGCACTCTT CACAGACACA 
GGCTACATCT GGATGGGGAC AGCATCACTC AAGGAGAAGC TATGTGAGTT CAACTGCAAC 
ATCCGGGCAC CTCCAAAGCA GATGGTCTGG TGCAGCCGTC CTCGTAGCAA GGAGAGGGCC 
GTGGTGGTGG CCTGGGAAAG GCGGCTGATG GTGGTGGGCG ATGCACCCGA GAGCATCCAG 
TTTGTGCTGG ATGAGGACTC CTACCTGGTG CCTGAGCTCG ATGGGGTCCG CATCTTCTCC 
CGCAGCACCC ACGAGTTCCT GCATGAGGTT CCAGCGGCCA GCGAGGAAAT CTTCAAAATT 
GCCTCAATGG CCCCCGGGGC GCTGCTCCTG GAGGCTCAGA AGGAGTATGA GAAAGAGAGC 
CAGAAGGCGG ACGAGTACCT GCGGGAGATC CAGGAGCTGG GCCAGCTGAC CCAGGCCGTG 
CAGCAGTGCA TTGAGGCTGC AGGACATGAG CACCAGCCAG ACATGCAGAA GAGTCTGCTC 
AGGGCCGCCT CCTTCGGAAA GTGTTTCCTG GACAGATTTC CACCCGACAG CTTCGTGCAC 
ATGTGTCAGG ACCTGCGTGT GCTCAATGCT GTTCGGGACT ATCACATCGG GATCCCGCTC 
ACCTATAGCC AATATAAGCA GCTCACCATC CAGGTGCTGC TGGACAGGCT CGTGTTGCGG 
AGACTTTACC CCCTGGCCAT CCAGATATGC GAGTACTTGC GCCTTCCTGA AGTACAGGGC 
GTCAGCAGGA TCCTGGCCCA CTGGGCCTGC TACAAGGTGC AACAGAAGGA TGTCTCAGAT 
GAGGATGTGG CTCGAGCCAT TAACCAGAAG CTGGGGGACA CGCCTGGTGT CTCTTACTCC 
GACATTGCTG CACGAGCCTA TGGTTGTGGC CGCACGGAGC TGGCCATCAA GCTGCTGGAG 
TATGAGCCAC GCTCAGGGGA GCAGGTACCC CTTCTCCTAA AGATGAAGAG GAGCAAACTG 
GCACTAAGCA AGGCCATCGA GAGCGGGGAC ACTGACCTGG TGTTCACGGT GTTGCTGCAC 
CTGAAGAACG AGCTGAACCG AGGAGATTTT TTCATGACCC TTCGGAATCA GCCCATGGCC 
CTCAGTTTGT ACCGACAGTT CTGTAAGCAT CAGGAGCTAG AGACGCTGAA GGACCTTTAC 
AATCAGGATG ACAATCACCA GGAATTGGGC AGCTTCCACA TCCGAGCCAG CTATGCTGCA 
GAAGAGCGTA TTGAGGGGCG AGTAGCAGCT CTGCAGACAG CCGCCGATGC CTTCTACAAG 
GCCAAGAATG AGTTTGCAGC CAAGGCTACA GAGGATCAAA TGCGGCTCCT ACGGCTGCAG 
CGGCGCCTAG AAGACGAGCT GGGGGGCCAG TTCCTAGACC TGTCTCTACA TGACACAGTT 
ACCACCCTCA TTCTTGGCGG TCACAACAAG CGTGCAGAGC AGCTGGCACG TGACTTCCGC 
ATCCCTGACA AGAGGCTCTG GTGGCTGAAG CTGACTGCCC TGGCAGATTT GGAAGATTGG 
GAAGAGCTAG AGAAGTTTTC CAAGAGCAAG AAATCACCCA TTGGCTACCT GCCTTTTGTG 
GAGATCTGCA TGAAACAACA TAACAAATAC GAAGCCAAGA AGTATGCTTC CCGCGTGGGT 
CCCGAGCAGA AGGTCAAGGC TTTGCTTCTT GTTGGCGATG TGGCTCAGGC TGCAGATGTG 
GCCATCGAAC ACCGGAATGA GGCTGAGCTG AGCCTCGTAT TGTCCCACTG CACGGGAGCC 
ACAGATGGGG CCACAGCTGA CAAGATTCAA CGGGCCAGGG CACAAGCCCA GAAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS16 ( NM_080413.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_536338.1 Homo sapiens VPS16 core subunit of CORVET and HOPS complexes (VPS16), transcript variant 3, mRNA.

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CloneID	OHu01249
Clone ID Related Accession (Same CDS sequence)	NM_080413.2
Accession Version	NM_080413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 16 homolog isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161656.20, CN483115.1, AK055787.1 and BC021291.2. On Sep 6, 2013 this sequence version replaced NM_080413.1. Transcript Variant: This variant (3) lacks an internal sequence segment compared to transcript variant 1. It thus encodes a shorter isoform (3) which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.132251.1, AK055787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTGCT ACACGGCGAA CTGGAACCCA CTCGGGGACT CTGCCTTTTA CCGGAAATAT 
GAGCTGTACA GCATGGACTG GGACCTGAAG GAGGAACTCA GGGATTGCCT GGTGGCTGCT 
GCACCCTATG GGGGCCCCAT TGCACTGCTG AGGAACCCCT GGCGGAAGGA GAAAGCTGCT 
AGTGTGAGGC CAGTGCTCGA TATATACTCT GCTTCCGGCA TGCCTCTGGC CAGCCTGCTG 
TGGAAGAGTG GACCCGTGGT GTCCCTGGGC TGGTCAGCTG AGGAGGAGCT GCTCTGTGTG 
CAGGAAGATG GTGCTGTACT GGTTTATGGG CTTCATGGTG ACTTCCGGAG ACACTTCAGC 
ATGGGCAATG AAGTGCTCCA GAACCGGGTT CTGGATGCCC GGATCTTTCA CACTGAGTTT 
GGTTCCGGAG TGGCCATCCT CACAGGGGCC CACCGCTTCA CCCTCAGTGC CAATGTGGGT 
GACCTCAAAC TCCGCCGGAT GCCAGAGGTG CCAGGTCTGC AAAGTGCACC CTCCTGCTGG 
ACTGTGCTGT GCCAGGACCG AGTGGCACAC ATTCTTCTGG CTGTGGGGCC TGACCTTTAC 
CTCTTGGACC ATGCAGCCTG CTCCGCAGTG ACGCCCCCTG GCCTGGCCCC AGGAGTAAGC 
AGCTTCCTAC AGATGGCTGT CTCCTTCACC TACCGACACC TGGCACTCTT CACAGACACA 
GGCTACATCT GGATGGGGAC AGCATCACTC AAGGAGAAGC TATGTGAGTT CAACTGCAAC 
ATCCGGGCAC CTCCAAAGCA GATGGTCTGG TGCAGCCGTC CTCGTAGCAA GGAGAGGGCC 
GTGGTGGTGG CCTGGGAAAG GCGGCTGATG GTGGTGGGCG ATGCACCCGA GAGCATCCAA 
TATAAGCAGC TCACCATCCA GGTGCTGCTG GACAGGCTCG TGTTGCGGAG ACTTTACCCC 
CTGGCCATCC AGATATGCGA GTACTTGCGC CTTCCTGAAG TACAGGGCGT CAGCAGGATC 
CTGGCCCACT GGGCCTGCTA CAAGGTGCAA CAGAAGGATG TCTCAGATGA GGATGTGGCT 
CGAGCCATTA ACCAGAAGCT GGGGGACACG CCTGGTGTCT CTTACTCCGA CATTGCTGCA 
CGAGCCTATG GTTGTGGCCG CACGGAGCTG GCCATCAAGC TGCTGGAGTA TGAGCCACGC 
TCAGGGGAGC AGGTACCCCT TCTCCTAAAG ATGAAGAGGA GCAAACTGGC ACTAAGCAAG 
GCCATCGAGA GCGGGGACAC TGACCTGGTG TTCACGGTGT TGCTGCACCT GAAGAACGAG 
CTGAACCGAG GAGATTTTTT CATGACCCTT CGGAATCAGC CCATGGCCCT CAGTTTGTAC 
CGACAGTTCT GTAAGCATCA GGAGCTAGAG ACGCTGAAGG ACCTTTACAA TCAGGATGAC 
AATCACCAGG AATTGGGCAG CTTCCACATC CGAGCCAGCT ATGCTGCAGA AGAGCGTATT 
GAGGGGCGAG TAGCAGCTCT GCAGACAGCC GCCGATGCCT TCTACAAGGC CAAGAATGAG 
TTTGCAGCCA AGGCTACAGA GGATCAAATG CGGCTCCTAC GGCTGCAGCG GCGCCTAGAA 
GACGAGCTGG GGGGCCAGTT CCTAGACCTG TCTCTACATG ACACAGTTAC CACCCTCATT 
CTTGGCGGTC ACAACAAGCG TGCAGAGCAG CTGGCACGTG ACTTCCGCAT CCCTGACAAG 
AGGCTCTGGT GGCTGAAGCT GACTGCCCTG GCAGATTTGG AAGATTGGGA AGAGCTAGAG 
AAGTTTTCCA AGAGCAAGAA ATCACCCATT GGCTACCTGC CTTTTGTGGA GATCTGCATG 
AAACAACATA ACAAATACGA AGCCAAGAAG TATGCTTCCC GCGTGGGTCC CGAGCAGAAG 
GTCAAGGCTT TGCTTCTTGT TGGCGATGTG GCTCAGGCTG CAGATGTGGC CATCGAACAC 
CGGAATGAGG CTGAGCTGAG CCTCGTATTG TCCCACTGCA CGGGAGCCAC AGATGGGGCC 
ACAGCTGACA AGATTCAACG GGCCAGGGCA CAAGCCCAGA AGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536338.1
CDS	79..2166
Translation	MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKAASVRPVLDIYSASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCWTVLCQDRVAHILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGDAPESIQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRLQRRLEDELGGQFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLTALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKYEAKKYASRVGPEQKVKALLLVGDVAQAADVAIEHRNEAELSLVLSHCTGATDGATADKIQRARAQAQKK

Target ORF information:

RefSeq Version	NM_080413.2
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS16 core subunit of CORVET and HOPS complexes (VPS16), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080413.2

ORF Insert Sequence:

ATGGACTGCT ACACGGCGAA CTGGAACCCA CTCGGGGACT CTGCCTTTTA CCGGAAATAT 
GAGCTGTACA GCATGGACTG GGACCTGAAG GAGGAACTCA GGGATTGCCT GGTGGCTGCT 
GCACCCTATG GGGGCCCCAT TGCACTGCTG AGGAACCCCT GGCGGAAGGA GAAAGCTGCT 
AGTGTGAGGC CAGTGCTCGA TATATACTCT GCTTCCGGCA TGCCTCTGGC CAGCCTGCTG 
TGGAAGAGTG GACCCGTGGT GTCCCTGGGC TGGTCAGCTG AGGAGGAGCT GCTCTGTGTG 
CAGGAAGATG GTGCTGTACT GGTTTATGGG CTTCATGGTG ACTTCCGGAG ACACTTCAGC 
ATGGGCAATG AAGTGCTCCA GAACCGGGTT CTGGATGCCC GGATCTTTCA CACTGAGTTT 
GGTTCCGGAG TGGCCATCCT CACAGGGGCC CACCGCTTCA CCCTCAGTGC CAATGTGGGT 
GACCTCAAAC TCCGCCGGAT GCCAGAGGTG CCAGGTCTGC AAAGTGCACC CTCCTGCTGG 
ACTGTGCTGT GCCAGGACCG AGTGGCACAC ATTCTTCTGG CTGTGGGGCC TGACCTTTAC 
CTCTTGGACC ATGCAGCCTG CTCCGCAGTG ACGCCCCCTG GCCTGGCCCC AGGAGTAAGC 
AGCTTCCTAC AGATGGCTGT CTCCTTCACC TACCGACACC TGGCACTCTT CACAGACACA 
GGCTACATCT GGATGGGGAC AGCATCACTC AAGGAGAAGC TATGTGAGTT CAACTGCAAC 
ATCCGGGCAC CTCCAAAGCA GATGGTCTGG TGCAGCCGTC CTCGTAGCAA GGAGAGGGCC 
GTGGTGGTGG CCTGGGAAAG GCGGCTGATG GTGGTGGGCG ATGCACCCGA GAGCATCCAA 
TATAAGCAGC TCACCATCCA GGTGCTGCTG GACAGGCTCG TGTTGCGGAG ACTTTACCCC 
CTGGCCATCC AGATATGCGA GTACTTGCGC CTTCCTGAAG TACAGGGCGT CAGCAGGATC 
CTGGCCCACT GGGCCTGCTA CAAGGTGCAA CAGAAGGATG TCTCAGATGA GGATGTGGCT 
CGAGCCATTA ACCAGAAGCT GGGGGACACG CCTGGTGTCT CTTACTCCGA CATTGCTGCA 
CGAGCCTATG GTTGTGGCCG CACGGAGCTG GCCATCAAGC TGCTGGAGTA TGAGCCACGC 
TCAGGGGAGC AGGTACCCCT TCTCCTAAAG ATGAAGAGGA GCAAACTGGC ACTAAGCAAG 
GCCATCGAGA GCGGGGACAC TGACCTGGTG TTCACGGTGT TGCTGCACCT GAAGAACGAG 
CTGAACCGAG GAGATTTTTT CATGACCCTT CGGAATCAGC CCATGGCCCT CAGTTTGTAC 
CGACAGTTCT GTAAGCATCA GGAGCTAGAG ACGCTGAAGG ACCTTTACAA TCAGGATGAC 
AATCACCAGG AATTGGGCAG CTTCCACATC CGAGCCAGCT ATGCTGCAGA AGAGCGTATT 
GAGGGGCGAG TAGCAGCTCT GCAGACAGCC GCCGATGCCT TCTACAAGGC CAAGAATGAG 
TTTGCAGCCA AGGCTACAGA GGATCAAATG CGGCTCCTAC GGCTGCAGCG GCGCCTAGAA 
GACGAGCTGG GGGGCCAGTT CCTAGACCTG TCTCTACATG ACACAGTTAC CACCCTCATT 
CTTGGCGGTC ACAACAAGCG TGCAGAGCAG CTGGCACGTG ACTTCCGCAT CCCTGACAAG 
AGGCTCTGGT GGCTGAAGCT GACTGCCCTG GCAGATTTGG AAGATTGGGA AGAGCTAGAG 
AAGTTTTCCA AGAGCAAGAA ATCACCCATT GGCTACCTGC CTTTTGTGGA GATCTGCATG 
AAACAACATA ACAAATACGA AGCCAAGAAG TATGCTTCCC GCGTGGGTCC CGAGCAGAAG 
GTCAAGGCTT TGCTTCTTGT TGGCGATGTG GCTCAGGCTG CAGATGTGGC CATCGAACAC 
CGGAATGAGG CTGAGCTGAG CCTCGTATTG TCCCACTGCA CGGGAGCCAC AGATGGGGCC 
ACAGCTGACA AGATTCAACG GGCCAGGGCA CAAGCCCAGA AGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms.
Human molecular genetics26(1)173-183(2017 01)
Kondo H,Maksimova N,Otomo T,Kato H,Imai A,Asano Y,Kobayashi K,Nojima S,Nakaya A,Hamada Y,Irahara K,Gurinova E,Sukhomyasova A,Nogovicina A,Savvina M,Yoshimori T,Ozono K,Sakai N

Homozygous mutation of VPS16 gene is responsible for an autosomal recessive adolescent-onset primary dystonia.
Scientific reports625834(2016 May)
Cai X,Chen X,Wu S,Liu W,Zhang X,Zhang D,He S,Wang B,Zhang M,Zhang Y,Li Z,Luo K,Cai Z,Li W

Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.
The Journal of biological chemistry290(51)30280-90(2015 Dec)
van der Kant R,Jonker CT,Wijdeven RH,Bakker J,Janssen L,Klumperman J,Neefjes J

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome Fusion with Endosomes and Autophagosomes.
Traffic (Copenhagen, Denmark)16(7)727-42(2015 Jul)
Wartosch L,G?nesdogan U,Graham SC,Luzio JP

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VPS16 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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