Vps16 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps16
Entrez Gene ID	80743
Full Name	VSP16 CORVET/HOPS core subunit
Synonyms	1810074M16Rik
General protein information	Preferred Names VSP16 CORVET/HOPS core subunit Names vacuolar protein sorting-associated protein 16 homolog mVPS16 vacuolar protein sorting 16
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 F1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006500430.3	XP_006500493.1	vacuolar protein sorting-associated protein 16 homolog isoform X1
XM_017319344.1	XP_017174833.1	vacuolar protein sorting-associated protein 16 homolog isoform X3
NM_001362651.1	NP_001349580.1	vacuolar protein sorting-associated protein 16 homolog isoform 2
XM_017319343.1	XP_017174832.1	vacuolar protein sorting-associated protein 16 homolog isoform X1
NM_030559.3	NP_085036.3	vacuolar protein sorting-associated protein 16 homolog isoform 1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000529	XP_310557.3
Arabidopsis thaliana (thale cress)	VCL1	NP_565879.1
Rattus norvegicus (Norway rat)	Vps16	NP_001005541.1
Danio rerio (zebrafish)	vps16	NP_001091659.1
Drosophila melanogaster (fruit fly)	Vps16A	NP_649877.1
Schizosaccharomyces pombe (fission yeast)	vps16	NP_593444.1
Neurospora crassa	NCU06268	XP_962872.2
Oryza sativa (rice)	Os01g0666800	NP_001043804.2
Homo sapiens (human)	VPS16	NP_072097.2
Bos taurus (cattle)	VPS16	NP_001015522.1
Gallus gallus (chicken)	VPS16	NP_001258079.1
Magnaporthe oryzae (rice blast fungus)	MGG_05256	XP_003712813.1
Xenopus tropicalis (tropical clawed frog)	vps16	NP_001004976.1
	VPS16	XP_002798262.1
Canis lupus familiaris (dog)	VPS16	XP_003639898.1
Mus musculus (house mouse)	Vps16	NP_085036.3

Gene Ontology
Bibliography

Related articles in PubMed

Homozygous mutation of VPS16 gene is responsible for an autosomal recessive adolescent-onset primary dystonia.
Cai X, Chen X, Wu S, Liu W, Zhang X, Zhang D, He S, Wang B, Zhang M, Zhang Y, Li Z, Luo K, Cai Z, Li W
Scientific reports625834(2016 May)

Mammalian CORVET is required for fusion and conversion of distinct early endosome subpopulations.
Perini ED, Schaefer R, St?ter M, Kalaidzidis Y, Zerial M
Traffic (Copenhagen, Denmark)15(12)1366-89(2014 Dec)

UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs.
Pirooz SD, He S, Zhang T, Zhang X, Zhao Z, Oh S, O'Connell D, Khalilzadeh P, Amini-Bavil-Olyaee S, Farzan M, Liang C
Proceedings of the National Academy of Sciences of the United States of America111(7)2716-21(2014 Feb)

Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.
Zlatic SA, Tornieri K, L'Hernault SW, Faundez V
Molecular biology of the cell22(10)1699-715(2011 May)

SPE-39 family proteins interact with the HOPS complex and function in lysosomal delivery.
Zhu GD, Salazar G, Zlatic SA, Fiza B, Doucette MM, Heilman CJ, Levey AI, Faundez V, L'hernault SW
Molecular biology of the cell20(4)1223-40(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Vps16p is highly expressed in neurons of various brain areas and developmental stages tested; it colocalizes with microtubule associated protein 2, but not glial fibrillary acidic protein.
Title: Expression of the mammalian homologue of vacuolar protein sorting 16 (Vps16p) in the mouse and rat brain.

mVps16p has potential roles in membrane trafficking
Title: Identification of mouse Vps16 and biochemical characterization of mammalian class C Vps complex.

The following Vps16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19693	NM_030559.3 Latest version!	Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$496.30 $709.00
OMu31038	XM_006500430.3 Latest version!	Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu80474	NM_001362651.1 Latest version!	Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu80473	XM_017319343.1 Latest version!	Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu80474	XM_017319344.1 Latest version!	Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps16 ( NM_030559.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_085036.3 Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19693
Clone ID Related Accession (Same CDS sequence)	NM_030559.3
Accession Version	NM_030559.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 16 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY123989.1, AK049872.1, BX890605.4 and AK032558.1. On Jul 31, 2009 this sequence version replaced NM_030559.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660813.7769.1, AK049872.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTGTT ACACTGCGAA CTGGAATCCA CTCGGGGACT CTGCCTTTTA CAGGAAGTAT 
GAGCTGTACA GCATGGACTG GGACCTGAAG GAGGAGCTCA AGGATTGCCT GGTGGCTGCT 
GCACCCTATG GGGGTCCCAT TGCTCTACTG AGGAACTGTT GGAGAAAAGA GAAAGCTGCC 
AGCGTGCGGC CAGTACTGGA GATCTACTCT GCATCTGGCT TACCACTGGC TAGCCTGCTG 
TGGAAGAGTG GGCCTGTGGT GGCCCTCGGC TGGTCAGCTG AGGAGGAGCT GCTCTGTGTG 
CAAGAAGATG GTGCAGTGCT AGTTTATGGC CTTCATGGAG ACTTCCGGAG GCACTTCAGC 
ATGGGCAATG AAGTGCTTCA GAACCGTGTC CTGGATGCCC GGATTTTTCA CACTGAGTTT 
GGTTCAGGGG TGGCCATCCT TACAGGAGCC TATCGCTTTA CTCTGAGTGC CAATGTGGGT 
GACCTCAAAC TCCGTCGGAT GCCAGAGGTG CCAGGTCTGC AAAGTGCACC CTCATGTTGG 
ACCACACTGT GCCACGACCG AGTACCACAC ATTCTTCTGG CTGTAGGACC CGATCTTTAC 
CTTCTGGATC ATGCTACCTG CTCTGCAGTG ACACCTGCTG GCTTAGCCCC AGGAGTGAGC 
AGCTTCCTAC AGATGGCTGT ATCCTTCACG TATCGTTACC TGGCACTCTT CACAGACACA 
GGCTACATCT GGATGGGGAC AGCATCACTC AAGGAGAAGC TGTGTGAGTT CAACTGCAAC 
ATCCGGGCTC CCCCGAAGCA GATGGTCTGG TGTAGCCGTC CTCGGAGCAA GGAAAGGGCT 
GTTGTTGTGG CCTGGGAGAG GCGGCTGATG GTGGTGGGCA ATGCGCCTGA AAGCATCCAG 
TTTGTGCTCG ATGAGGACTC CTACTTAGTG CCTGAGCTTG ATGGGGTCCG CATCTTCTCC 
CGAAGCACCC ATGAGTTCCT GCATGAAGTC CCAGTGGCCA GTGAAGAGAT CTTCAAAATT 
GCCTCAATGG CCCCTGGAGC ACTGCTGTTA GAGGCCCAGA AGGAATATGA GAAAGAGAGC 
CAGAAAGCAG ATGAGTACCT GCGGGAGATC CAGGAACTGG GCCAGCTGAT CCAGGCTGTG 
CAGCAGTGCA TTGAGGCTGC AGGACATGAG CACCAGCCAG ACATGCAAAA GAGTCTGCTC 
AGGGCGGCTT CATTTGGAAA GTGTTTCCTT GACAGATTTC CACCTGACAG CTTCGTGCAC 
ATGTGTCAGG ACCTTCGAGT GCTCAATGCT ATTAGAGACT ACCACATTGG GATCCCTCTC 
ACCTACACCC AATACAAGCA GCTCACCATC CAGGTGCTGC TAGACAGGCT TGTGTTGCGG 
AGGCTTTATC CCCTGGCTAT CCAGATATGC GAGTACCTGC GCCTTCCTGA AGTCCAGGGT 
GTCAGCAGGA TCCTAGCCCA CTGGGCCTGC TACAAGGTGC AGCAGAAAGA TGTCTCAGAT 
GAAGATGTAG CACGGGCTAT TAATCAGAAG CTGGGTGACA CGCCTGGTGT TTCATACTCT 
GACATTGCTG CGAGGGCCTA TGGCTGTGGC CGCACTGAAC TGGCCATTAA GCTGCTGGAG 
TATGAGCCAC GCTCAGGGGA GCAGGTACCT CTTCTCCTAA AGATGAAGAG GAGCAAACTG 
GCACTGAGCA AGGCTATTGA GAGTGGAGAC ACTGACCTGG TGTTTACAGT GTTGCTGCAC 
CTAAAGAATG AACTCAACCG AGGAGACTTT TTCATGACTC TCCGGAACCA ACCCATGGCC 
CTGAGTTTGT ATCGACAGTT CTGTAAGCAT CAGGAGCTAG ACACTCTGAA GGACCTTTAC 
AATCAGGACG ACAATCACCA GGAGTTGGGC AGCTTCCACA TCAGAGCCAG CTATGCCGCT 
GAAGAGCGTA TTGAAGGGCG AGTGGCAGCT CTGCAGACGG CAGCTGATGC CTTCTACAAA 
GCCAAGAATG AATTTGCAGC CAAGGCCACA GAGGATCAAA TGAGGCTCCT GCGGATACAG 
CGACGCCTAG AGGATGAGCT AGGGGGCAGG TTCCTAGACC TATCTCTACA CGACACTGTC 
ACCACTCTTA TCCTTGGAGG CCACAACAAG CGGGCAGAGC AGCTGGCACG TGACTTTCGC 
ATCCCTGATA AGAGACTATG GTGGTTAAAG CTGGCTGCCC TGGCAGATTT GGAAGATTGG 
GAGGAGCTGG AGAAGTTTTC CAAAAGCAAG AAATCACCGA TTGGTTACCT GCCCTTTGTA 
GAGATCTGCA TGAAACAGCA TAACAAACAT GAGGCCAAGA AGTATGCTTC CCGTGTGGGC 
CCTGAACAAA AGGTCAAGGC TTTGCTTCTT GTTGGGGATG TGGCTCAGGC TGCAGAGGTG 
GCTATTGAGC ACCGGAATGA AACAGAGCTG AGCCTCGTTT TGTCCCACTG CACTGGAGCC 
ACAGATGGGG CCATAGCTGA CAAGATTCAG CGGGCCAGGG CACAAGCCCA GAAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_085036.3
CDS	76..2595
Translation	MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELKDCLVAAAPYGGPIALLRNCWRKEKAASVRPVLEIYSASGLPLASLLWKSGPVVALGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAYRFTLSANVGDLKLRRMPEVPGLQSAPSCWTTLCHDRVPHILLAVGPDLYLLDHATCSAVTPAGLAPGVSSFLQMAVSFTYRYLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGNAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPVASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLIQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAIRDYHIGIPLTYTQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELDTLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRIQRRLEDELGGRFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLAALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKHEAKKYASRVGPEQKVKALLLVGDVAQAAEVAIEHRNETELSLVLSHCTGATDGAIADKIQRARAQAQKK

Target ORF information:

RefSeq Version	NM_030559.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030559.3

ORF Insert Sequence:

ATGGACTGTT ACACTGCGAA CTGGAATCCA CTCGGGGACT CTGCCTTTTA CAGGAAGTAT 
GAGCTGTACA GCATGGACTG GGACCTGAAG GAGGAGCTCA AGGATTGCCT GGTGGCTGCT 
GCACCCTATG GGGGTCCCAT TGCTCTACTG AGGAACTGTT GGAGAAAAGA GAAAGCTGCC 
AGCGTGCGGC CAGTACTGGA GATCTACTCT GCATCTGGCT TACCACTGGC TAGCCTGCTG 
TGGAAGAGTG GGCCTGTGGT GGCCCTCGGC TGGTCAGCTG AGGAGGAGCT GCTCTGTGTG 
CAAGAAGATG GTGCAGTGCT AGTTTATGGC CTTCATGGAG ACTTCCGGAG GCACTTCAGC 
ATGGGCAATG AAGTGCTTCA GAACCGTGTC CTGGATGCCC GGATTTTTCA CACTGAGTTT 
GGTTCAGGGG TGGCCATCCT TACAGGAGCC TATCGCTTTA CTCTGAGTGC CAATGTGGGT 
GACCTCAAAC TCCGTCGGAT GCCAGAGGTG CCAGGTCTGC AAAGTGCACC CTCATGTTGG 
ACCACACTGT GCCACGACCG AGTACCACAC ATTCTTCTGG CTGTAGGACC CGATCTTTAC 
CTTCTGGATC ATGCTACCTG CTCTGCAGTG ACACCTGCTG GCTTAGCCCC AGGAGTGAGC 
AGCTTCCTAC AGATGGCTGT ATCCTTCACG TATCGTTACC TGGCACTCTT CACAGACACA 
GGCTACATCT GGATGGGGAC AGCATCACTC AAGGAGAAGC TGTGTGAGTT CAACTGCAAC 
ATCCGGGCTC CCCCGAAGCA GATGGTCTGG TGTAGCCGTC CTCGGAGCAA GGAAAGGGCT 
GTTGTTGTGG CCTGGGAGAG GCGGCTGATG GTGGTGGGCA ATGCGCCTGA AAGCATCCAG 
TTTGTGCTCG ATGAGGACTC CTACTTAGTG CCTGAGCTTG ATGGGGTCCG CATCTTCTCC 
CGAAGCACCC ATGAGTTCCT GCATGAAGTC CCAGTGGCCA GTGAAGAGAT CTTCAAAATT 
GCCTCAATGG CCCCTGGAGC ACTGCTGTTA GAGGCCCAGA AGGAATATGA GAAAGAGAGC 
CAGAAAGCAG ATGAGTACCT GCGGGAGATC CAGGAACTGG GCCAGCTGAT CCAGGCTGTG 
CAGCAGTGCA TTGAGGCTGC AGGACATGAG CACCAGCCAG ACATGCAAAA GAGTCTGCTC 
AGGGCGGCTT CATTTGGAAA GTGTTTCCTT GACAGATTTC CACCTGACAG CTTCGTGCAC 
ATGTGTCAGG ACCTTCGAGT GCTCAATGCT ATTAGAGACT ACCACATTGG GATCCCTCTC 
ACCTACACCC AATACAAGCA GCTCACCATC CAGGTGCTGC TAGACAGGCT TGTGTTGCGG 
AGGCTTTATC CCCTGGCTAT CCAGATATGC GAGTACCTGC GCCTTCCTGA AGTCCAGGGT 
GTCAGCAGGA TCCTAGCCCA CTGGGCCTGC TACAAGGTGC AGCAGAAAGA TGTCTCAGAT 
GAAGATGTAG CACGGGCTAT TAATCAGAAG CTGGGTGACA CGCCTGGTGT TTCATACTCT 
GACATTGCTG CGAGGGCCTA TGGCTGTGGC CGCACTGAAC TGGCCATTAA GCTGCTGGAG 
TATGAGCCAC GCTCAGGGGA GCAGGTACCT CTTCTCCTAA AGATGAAGAG GAGCAAACTG 
GCACTGAGCA AGGCTATTGA GAGTGGAGAC ACTGACCTGG TGTTTACAGT GTTGCTGCAC 
CTAAAGAATG AACTCAACCG AGGAGACTTT TTCATGACTC TCCGGAACCA ACCCATGGCC 
CTGAGTTTGT ATCGACAGTT CTGTAAGCAT CAGGAGCTAG ACACTCTGAA GGACCTTTAC 
AATCAGGACG ACAATCACCA GGAGTTGGGC AGCTTCCACA TCAGAGCCAG CTATGCCGCT 
GAAGAGCGTA TTGAAGGGCG AGTGGCAGCT CTGCAGACGG CAGCTGATGC CTTCTACAAA 
GCCAAGAATG AATTTGCAGC CAAGGCCACA GAGGATCAAA TGAGGCTCCT GCGGATACAG 
CGACGCCTAG AGGATGAGCT AGGGGGCAGG TTCCTAGACC TATCTCTACA CGACACTGTC 
ACCACTCTTA TCCTTGGAGG CCACAACAAG CGGGCAGAGC AGCTGGCACG TGACTTTCGC 
ATCCCTGATA AGAGACTATG GTGGTTAAAG CTGGCTGCCC TGGCAGATTT GGAAGATTGG 
GAGGAGCTGG AGAAGTTTTC CAAAAGCAAG AAATCACCGA TTGGTTACCT GCCCTTTGTA 
GAGATCTGCA TGAAACAGCA TAACAAACAT GAGGCCAAGA AGTATGCTTC CCGTGTGGGC 
CCTGAACAAA AGGTCAAGGC TTTGCTTCTT GTTGGGGATG TGGCTCAGGC TGCAGAGGTG 
GCTATTGAGC ACCGGAATGA AACAGAGCTG AGCCTCGTTT TGTCCCACTG CACTGGAGCC 
ACAGATGGGG CCATAGCTGA CAAGATTCAG CGGGCCAGGG CACAAGCCCA GAAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps16 ( XM_006500430.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500493.1 Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31038
Clone ID Related Accession (Same CDS sequence)	XM_006500430.3
Accession Version	XM_006500430.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 16 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500430.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGTT ACACTGCGAA CTGGAATCCA CTCGGGGACT CTGCCTTTTA CAGGAAGTAT 
GAGCTGTACA GCATGGACTG GGACCTGAAG GAGGAGCTCA AGGATTGCCT GGTGGCTGCT 
GCACCCTATG GGGGTCCCAT TGCTCTACTG AGGAACTGTT GGAGAAAAGA GAAAGCTGCC 
AGCGTGCGGC CAGTACTGGA GATCTACTCT GCATCTGGCT TACCACTGGC TAGCCTGCTG 
TGGAAGAGTG GGCCTGTGGT GGCCCTCGGC TGGTCAGCTG AGGAGGAGCT GCTCTGTGTG 
CAAGAAGATG GTGCAGTGCT AGTTTATGGC CTTCATGGAG ACTTCCGGAG GCACTTCAGC 
ATGGGCAATG AAGTGCTTCA GAACCGTGTC CTGGATGCCC GGATTTTTCA CACTGAGTTT 
GGTTCAGGGG TGGCCATCCT TACAGGAGCC TATCGCTTTA CTCTGAGTGC CAATGTGGGT 
GACCTCAAAC TCCGTCGGAT GCCAGAGGTG CCAGGTCTGC AAAGTGCACC CTCATGTTGG 
ACCACACTGT GCCACGACCG AGTACCACAC ATTCTTCTGG CTGTAGGACC CGATCTTTAC 
CTTCTGGATC ATGCTACCTG CTCTGCAGTG ACACCTGCTG GCTTAGCCCC AGGAGTGAGC 
AGCTTCCTAC AGATGGCTGT ATCCTTCACG TATCGTTACC TGGCACTCTT CACAGACACA 
GGCTACATCT GGATGGGGAC AGCATCACTC AAGGAGAAGC TGTGTGAGTT CAACTGCAAC 
ATCCGGGCTC CCCCGAAGCA GATGGTCTGG TGTAGCCGTC CTCGGAGCAA GGAAAGGGCT 
GTTGTTGTGG CCTGGGAGAG GCGGCTGATG GTGGTGGGCA ATGCGCCTGA AAGCATCCAG 
TTTGTGCTCG ATGAGGACTC CTACTTAGTG CCTGAGCTTG ATGGGGTCCG CATCTTCTCC 
CGAAGCACCC ATGAGTTCCT GCATGAAGTC CCAGTGGCCA GTGAAGAGAT CTTCAAAATT 
GCCTCAATGG CCCCTGGAGC ACTGCTGTTA GAGGCCCAGA AGGAATATGA GAAAGAGAGC 
CAGAAAGCAG ATGAGTACCT GCGGGAGATC CAGGAACTGG GCCAGCTGAT CCAGGCTGTG 
CAGCAGTGCA TTGAGGCTGC AGGACATGAG CACCAGCCAG ACATGCAAAA GAGTCTGCTC 
AGGGCGGCTT CATTTGGAAA GTGTTTCCTT GACAGATTTC CACCTGACAG CTTCGTGCAC 
ATGTGTCAGG ACCTTCGAGT GCTCAATGCT ATTAGAGACT ACCACATTGG GATCCCTCTC 
ACCTACACCC AATACAAGCA GCTCACCATC CAGGTGCTGC TAGACAGGCT TGTGTTGCGG 
AGGCTTTATC CCCTGGCTAT CCAGATATGC GAGTACCTGC GCCTTCCTGA AGTCCAGGGT 
GTCAGCAGGA TCCTAGCCCA CTGGGCCTGC TACAAGGTGC AGCAGAAAGA TGTCTCAGAT 
GAAGATGTAG CACGGGCTAT TAATCAGAAG CTGGGTGACA CGCCTGGTGT TTCATACTCT 
GACATTGCTG CGAGGGCCTA TGGCTGTGGC CGCACTGAAC TGGCCATTAA GCTGCTGGAG 
TATGAGCCAC GCTCAGGGGA GCAGGTACCT CTTCTCCTAA AGATGAAGAG GAGCAAACTG 
GCACTGAGCA AGGCTATTGA GAGTGGAGAC ACTGACCTGG TGTTGCTGCA CCTAAAGAAT 
GAACTCAACC GAGGAGACTT TTTCATGACT CTCCGGAACC AACCCATGGC CCTGAGTTTG 
TATCGACAGT TCTGTAAGCA TCAGGAGCTA GACACTCTGA AGGACCTTTA CAATCAGGAC 
GACAATCACC AGGAGTTGGG CAGCTTCCAC ATCAGAGCCA GCTATGCCGC TGAAGAGCGT 
ATTGAAGGGC GAGTGGCAGC TCTGCAGACG GCAGCTGATG CCTTCTACAA AGCCAAGAAT 
GAATTTGCAG CCAAGGCCAC AGAGGATCAA ATGAGGCTCC TGCGGATACA GCGACGCCTA 
GAGGATGAGC TAGGGGGCAG GTTCCTAGAC CTATCTCTAC ACGACACTGT CACCACTCTT 
ATCCTTGGAG GCCACAACAA GCGGGCAGAG CAGCTGGCAC GTGACTTTCG CATCCCTGAT 
AAGAGACTAT GGTGGTTAAA GCTGGCTGCC CTGGCAGATT TGGAAGATTG GGAGGAGCTG 
GAGAAGTTTT CCAAAAGCAA GAAATCACCG ATTGGTTACC TGCCCTTTGT AGAGATCTGC 
ATGAAACAGC ATAACAAACA TGAGGCCAAG AAGTATGCTT CCCGTGTGGG CCCTGAACAA 
AAGGTCAAGG CTTTGCTTCT TGTTGGGGAT GTGGCTCAGG CTGCAGAGGT GGCTATTGAG 
CACCGGAATG AAACAGAGCT GAGCCTCGTT TTGTCCCACT GCACTGGAGC CACAGATGGG 
GCCATAGCTG ACAAGATTCA GCGGGCCAGG GCACAAGCCC AGAAGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500493.1
CDS	76..2586
Translation	MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELKDCLVAAAPYGGPIALLRNCWRKEKAASVRPVLEIYSASGLPLASLLWKSGPVVALGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAYRFTLSANVGDLKLRRMPEVPGLQSAPSCWTTLCHDRVPHILLAVGPDLYLLDHATCSAVTPAGLAPGVSSFLQMAVSFTYRYLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGNAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPVASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLIQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAIRDYHIGIPLTYTQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELDTLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRIQRRLEDELGGRFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLAALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKHEAKKYASRVGPEQKVKALLLVGDVAQAAEVAIEHRNETELSLVLSHCTGATDGAIADKIQRARAQAQKK

Target ORF information:

RefSeq Version	XM_006500430.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500430.3

ORF Insert Sequence:

ATGGACTGTT ACACTGCGAA CTGGAATCCA CTCGGGGACT CTGCCTTTTA CAGGAAGTAT 
GAGCTGTACA GCATGGACTG GGACCTGAAG GAGGAGCTCA AGGATTGCCT GGTGGCTGCT 
GCACCCTATG GGGGTCCCAT TGCTCTACTG AGGAACTGTT GGAGAAAAGA GAAAGCTGCC 
AGCGTGCGGC CAGTACTGGA GATCTACTCT GCATCTGGCT TACCACTGGC TAGCCTGCTG 
TGGAAGAGTG GGCCTGTGGT GGCCCTCGGC TGGTCAGCTG AGGAGGAGCT GCTCTGTGTG 
CAAGAAGATG GTGCAGTGCT AGTTTATGGC CTTCATGGAG ACTTCCGGAG GCACTTCAGC 
ATGGGCAATG AAGTGCTTCA GAACCGTGTC CTGGATGCCC GGATTTTTCA CACTGAGTTT 
GGTTCAGGGG TGGCCATCCT TACAGGAGCC TATCGCTTTA CTCTGAGTGC CAATGTGGGT 
GACCTCAAAC TCCGTCGGAT GCCAGAGGTG CCAGGTCTGC AAAGTGCACC CTCATGTTGG 
ACCACACTGT GCCACGACCG AGTACCACAC ATTCTTCTGG CTGTAGGACC CGATCTTTAC 
CTTCTGGATC ATGCTACCTG CTCTGCAGTG ACACCTGCTG GCTTAGCCCC AGGAGTGAGC 
AGCTTCCTAC AGATGGCTGT ATCCTTCACG TATCGTTACC TGGCACTCTT CACAGACACA 
GGCTACATCT GGATGGGGAC AGCATCACTC AAGGAGAAGC TGTGTGAGTT CAACTGCAAC 
ATCCGGGCTC CCCCGAAGCA GATGGTCTGG TGTAGCCGTC CTCGGAGCAA GGAAAGGGCT 
GTTGTTGTGG CCTGGGAGAG GCGGCTGATG GTGGTGGGCA ATGCGCCTGA AAGCATCCAG 
TTTGTGCTCG ATGAGGACTC CTACTTAGTG CCTGAGCTTG ATGGGGTCCG CATCTTCTCC 
CGAAGCACCC ATGAGTTCCT GCATGAAGTC CCAGTGGCCA GTGAAGAGAT CTTCAAAATT 
GCCTCAATGG CCCCTGGAGC ACTGCTGTTA GAGGCCCAGA AGGAATATGA GAAAGAGAGC 
CAGAAAGCAG ATGAGTACCT GCGGGAGATC CAGGAACTGG GCCAGCTGAT CCAGGCTGTG 
CAGCAGTGCA TTGAGGCTGC AGGACATGAG CACCAGCCAG ACATGCAAAA GAGTCTGCTC 
AGGGCGGCTT CATTTGGAAA GTGTTTCCTT GACAGATTTC CACCTGACAG CTTCGTGCAC 
ATGTGTCAGG ACCTTCGAGT GCTCAATGCT ATTAGAGACT ACCACATTGG GATCCCTCTC 
ACCTACACCC AATACAAGCA GCTCACCATC CAGGTGCTGC TAGACAGGCT TGTGTTGCGG 
AGGCTTTATC CCCTGGCTAT CCAGATATGC GAGTACCTGC GCCTTCCTGA AGTCCAGGGT 
GTCAGCAGGA TCCTAGCCCA CTGGGCCTGC TACAAGGTGC AGCAGAAAGA TGTCTCAGAT 
GAAGATGTAG CACGGGCTAT TAATCAGAAG CTGGGTGACA CGCCTGGTGT TTCATACTCT 
GACATTGCTG CGAGGGCCTA TGGCTGTGGC CGCACTGAAC TGGCCATTAA GCTGCTGGAG 
TATGAGCCAC GCTCAGGGGA GCAGGTACCT CTTCTCCTAA AGATGAAGAG GAGCAAACTG 
GCACTGAGCA AGGCTATTGA GAGTGGAGAC ACTGACCTGG TGTTGCTGCA CCTAAAGAAT 
GAACTCAACC GAGGAGACTT TTTCATGACT CTCCGGAACC AACCCATGGC CCTGAGTTTG 
TATCGACAGT TCTGTAAGCA TCAGGAGCTA GACACTCTGA AGGACCTTTA CAATCAGGAC 
GACAATCACC AGGAGTTGGG CAGCTTCCAC ATCAGAGCCA GCTATGCCGC TGAAGAGCGT 
ATTGAAGGGC GAGTGGCAGC TCTGCAGACG GCAGCTGATG CCTTCTACAA AGCCAAGAAT 
GAATTTGCAG CCAAGGCCAC AGAGGATCAA ATGAGGCTCC TGCGGATACA GCGACGCCTA 
GAGGATGAGC TAGGGGGCAG GTTCCTAGAC CTATCTCTAC ACGACACTGT CACCACTCTT 
ATCCTTGGAG GCCACAACAA GCGGGCAGAG CAGCTGGCAC GTGACTTTCG CATCCCTGAT 
AAGAGACTAT GGTGGTTAAA GCTGGCTGCC CTGGCAGATT TGGAAGATTG GGAGGAGCTG 
GAGAAGTTTT CCAAAAGCAA GAAATCACCG ATTGGTTACC TGCCCTTTGT AGAGATCTGC 
ATGAAACAGC ATAACAAACA TGAGGCCAAG AAGTATGCTT CCCGTGTGGG CCCTGAACAA 
AAGGTCAAGG CTTTGCTTCT TGTTGGGGAT GTGGCTCAGG CTGCAGAGGT GGCTATTGAG 
CACCGGAATG AAACAGAGCT GAGCCTCGTT TTGTCCCACT GCACTGGAGC CACAGATGGG 
GCCATAGCTG ACAAGATTCA GCGGGCCAGG GCACAAGCCC AGAAGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps16 ( NM_001362651.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349580.1 Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant 2, mRNA.

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CloneID	OMu80474
Clone ID Related Accession (Same CDS sequence)	XM_017319344.1 , NM_001362651.1
Accession Version	NM_001362651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 16 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX890605.4. On Apr 24, 2018 this sequence version replaced XM_017319344.1. Transcript Variant: This variant (2) lacks an alternate exon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.150717.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGTGG GCAATGCGCC TGAAAGCATC CAGTTTGTGC TCGATGAGGA CTCCTACTTA 
GTGCCTGAGC TTGATGGGGT CCGCATCTTC TCCCGAAGCA CCCATGAGTT CCTGCATGAA 
GTCCCAGTGG CCAGTGAAGA GATCTTCAAA ATTGCCTCAA TGGCCCCTGG AGCACTGCTG 
TTAGAGGCCC AGAAGGAATA TGAGAAAGAG AGCCAGAAAG CAGATGAGTA CCTGCGGGAG 
ATCCAGGAAC TGGGCCAGCT GATCCAGGCT GTGCAGCAGT GCATTGAGGC TGCAGGACAT 
GAGCACCAGC CAGACATGCA AAAGAGTCTG CTCAGGGCGG CTTCATTTGG AAAGTGTTTC 
CTTGACAGAT TTCCACCTGA CAGCTTCGTG CACATGTGTC AGGACCTTCG AGTGCTCAAT 
GCTATTAGAG ACTACCACAT TGGGATCCCT CTCACCTACA CCCAATACAA GCAGCTCACC 
ATCCAGGTGC TGCTAGACAG GCTTGTGTTG CGGAGGCTTT ATCCCCTGGC TATCCAGATA 
TGCGAGTACC TGCGCCTTCC TGAAGTCCAG GGTGTCAGCA GGATCCTAGC CCACTGGGCC 
TGCTACAAGG TGCAGCAGAA AGATGTCTCA GATGAAGATG TAGCACGGGC TATTAATCAG 
AAGCTGGGTG ACACGCCTGG TGTTTCATAC TCTGACATTG CTGCGAGGGC CTATGGCTGT 
GGCCGCACTG AACTGGCCAT TAAGCTGCTG GAGTATGAGC CACGCTCAGG GGAGCAGGTA 
CCTCTTCTCC TAAAGATGAA GAGGAGCAAA CTGGCACTGA GCAAGGCTAT TGAGAGTGGA 
GACACTGACC TGGTGTTGCT GCACCTAAAG AATGAACTCA ACCGAGGAGA CTTTTTCATG 
ACTCTCCGGA ACCAACCCAT GGCCCTGAGT TTGTATCGAC AGTTCTGTAA GCATCAGGAG 
CTAGACACTC TGAAGGACCT TTACAATCAG GACGACAATC ACCAGGAGTT GGGCAGCTTC 
CACATCAGAG CCAGCTATGC CGCTGAAGAG CGTATTGAAG GGCGAGTGGC AGCTCTGCAG 
ACGGCAGCTG ATGCCTTCTA CAAAGCCAAG AATGAATTTG CAGCCAAGGC CACAGAGGAT 
CAAATGAGGC TCCTGCGGAT ACAGCGACGC CTAGAGGATG AGCTAGGGGG CAGGTTCCTA 
GACCTATCTC TACACGACAC TGTCACCACT CTTATCCTTG GAGGCCACAA CAAGCGGGCA 
GAGCAGCTGG CACGTGACTT TCGCATCCCT GATAAGAGAC TATGGTGGTT AAAGCTGGCT 
GCCCTGGCAG ATTTGGAAGA TTGGGAGGAG CTGGAGAAGT TTTCCAAAAG CAAGAAATCA 
CCGATTGGTT ACCTGCCCTT TGTAGAGATC TGCATGAAAC AGCATAACAA ACATGAGGCC 
AAGAAGTATG CTTCCCGTGT GGGCCCTGAA CAAAAGGTCA AGGCTTTGCT TCTTGTTGGG 
GATGTGGCTC AGGCTGCAGA GGTGGCTATT GAGCACCGGA ATGAAACAGA GCTGAGCCTC 
GTTTTGTCCC ACTGCACTGG AGCCACAGAT GGGGCCATAG CTGACAAGAT TCAGCGGGCC 
AGGGCACAAG CCCAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349580.1
CDS	887..2530
Translation	MVVGNAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPVASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLIQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAIRDYHIGIPLTYTQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELDTLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRIQRRLEDELGGRFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLAALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKHEAKKYASRVGPEQKVKALLLVGDVAQAAEVAIEHRNETELSLVLSHCTGATDGAIADKIQRARAQAQKK

Target ORF information:

RefSeq Version	NM_001362651.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362651.1

ORF Insert Sequence:

ATGGTGGTGG GCAATGCGCC TGAAAGCATC CAGTTTGTGC TCGATGAGGA CTCCTACTTA 
GTGCCTGAGC TTGATGGGGT CCGCATCTTC TCCCGAAGCA CCCATGAGTT CCTGCATGAA 
GTCCCAGTGG CCAGTGAAGA GATCTTCAAA ATTGCCTCAA TGGCCCCTGG AGCACTGCTG 
TTAGAGGCCC AGAAGGAATA TGAGAAAGAG AGCCAGAAAG CAGATGAGTA CCTGCGGGAG 
ATCCAGGAAC TGGGCCAGCT GATCCAGGCT GTGCAGCAGT GCATTGAGGC TGCAGGACAT 
GAGCACCAGC CAGACATGCA AAAGAGTCTG CTCAGGGCGG CTTCATTTGG AAAGTGTTTC 
CTTGACAGAT TTCCACCTGA CAGCTTCGTG CACATGTGTC AGGACCTTCG AGTGCTCAAT 
GCTATTAGAG ACTACCACAT TGGGATCCCT CTCACCTACA CCCAATACAA GCAGCTCACC 
ATCCAGGTGC TGCTAGACAG GCTTGTGTTG CGGAGGCTTT ATCCCCTGGC TATCCAGATA 
TGCGAGTACC TGCGCCTTCC TGAAGTCCAG GGTGTCAGCA GGATCCTAGC CCACTGGGCC 
TGCTACAAGG TGCAGCAGAA AGATGTCTCA GATGAAGATG TAGCACGGGC TATTAATCAG 
AAGCTGGGTG ACACGCCTGG TGTTTCATAC TCTGACATTG CTGCGAGGGC CTATGGCTGT 
GGCCGCACTG AACTGGCCAT TAAGCTGCTG GAGTATGAGC CACGCTCAGG GGAGCAGGTA 
CCTCTTCTCC TAAAGATGAA GAGGAGCAAA CTGGCACTGA GCAAGGCTAT TGAGAGTGGA 
GACACTGACC TGGTGTTGCT GCACCTAAAG AATGAACTCA ACCGAGGAGA CTTTTTCATG 
ACTCTCCGGA ACCAACCCAT GGCCCTGAGT TTGTATCGAC AGTTCTGTAA GCATCAGGAG 
CTAGACACTC TGAAGGACCT TTACAATCAG GACGACAATC ACCAGGAGTT GGGCAGCTTC 
CACATCAGAG CCAGCTATGC CGCTGAAGAG CGTATTGAAG GGCGAGTGGC AGCTCTGCAG 
ACGGCAGCTG ATGCCTTCTA CAAAGCCAAG AATGAATTTG CAGCCAAGGC CACAGAGGAT 
CAAATGAGGC TCCTGCGGAT ACAGCGACGC CTAGAGGATG AGCTAGGGGG CAGGTTCCTA 
GACCTATCTC TACACGACAC TGTCACCACT CTTATCCTTG GAGGCCACAA CAAGCGGGCA 
GAGCAGCTGG CACGTGACTT TCGCATCCCT GATAAGAGAC TATGGTGGTT AAAGCTGGCT 
GCCCTGGCAG ATTTGGAAGA TTGGGAGGAG CTGGAGAAGT TTTCCAAAAG CAAGAAATCA 
CCGATTGGTT ACCTGCCCTT TGTAGAGATC TGCATGAAAC AGCATAACAA ACATGAGGCC 
AAGAAGTATG CTTCCCGTGT GGGCCCTGAA CAAAAGGTCA AGGCTTTGCT TCTTGTTGGG 
GATGTGGCTC AGGCTGCAGA GGTGGCTATT GAGCACCGGA ATGAAACAGA GCTGAGCCTC 
GTTTTGTCCC ACTGCACTGG AGCCACAGAT GGGGCCATAG CTGACAAGAT TCAGCGGGCC 
AGGGCACAAG CCCAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps16 ( XM_017319343.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174832.1 Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant X1, mRNA.

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CloneID	OMu80473
Clone ID Related Accession (Same CDS sequence)	XM_017319343.1
Accession Version	XM_017319343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 16 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGTT ACACTGCGAA CTGGAATCCA CTCGGGGACT CTGCCTTTTA CAGGAAGTAT 
GAGCTGTACA GCATGGACTG GGACCTGAAG GAGGAGCTCA AGGATTGCCT GGTGGCTGCT 
GCACCCTATG GGGGTCCCAT TGCTCTACTG AGGAACTGTT GGAGAAAAGA GAAAGCTGCC 
AGCGTGCGGC CAGTACTGGA GATCTACTCT GCATCTGGCT TACCACTGGC TAGCCTGCTG 
TGGAAGAGTG GGCCTGTGGT GGCCCTCGGC TGGTCAGCTG AGGAGGAGCT GCTCTGTGTG 
CAAGAAGATG GTGCAGTGCT AGTTTATGGC CTTCATGGAG ACTTCCGGAG GCACTTCAGC 
ATGGGCAATG AAGTGCTTCA GAACCGTGTC CTGGATGCCC GGATTTTTCA CACTGAGTTT 
GGTTCAGGGG TGGCCATCCT TACAGGAGCC TATCGCTTTA CTCTGAGTGC CAATGTGGGT 
GACCTCAAAC TCCGTCGGAT GCCAGAGGTG CCAGGTCTGC AAAGTGCACC CTCATGTTGG 
ACCACACTGT GCCACGACCG AGTACCACAC ATTCTTCTGG CTGTAGGACC CGATCTTTAC 
CTTCTGGATC ATGCTACCTG CTCTGCAGTG ACACCTGCTG GCTTAGCCCC AGGAGTGAGC 
AGCTTCCTAC AGATGGCTGT ATCCTTCACG TATCGTTACC TGGCACTCTT CACAGACACA 
GGCTACATCT GGATGGGGAC AGCATCACTC AAGGAGAAGC TGTGTGAGTT CAACTGCAAC 
ATCCGGGCTC CCCCGAAGCA GATGGTCTGG TGTAGCCGTC CTCGGAGCAA GGAAAGGGCT 
GTTGTTGTGG CCTGGGAGAG GCGGCTGATG GTGGTGGGCA ATGCGCCTGA AAGCATCCAG 
TTTGTGCTCG ATGAGGACTC CTACTTAGTG CCTGAGCTTG ATGGGGTCCG CATCTTCTCC 
CGAAGCACCC ATGAGTTCCT GCATGAAGTC CCAGTGGCCA GTGAAGAGAT CTTCAAAATT 
GCCTCAATGG CCCCTGGAGC ACTGCTGTTA GAGGCCCAGA AGGAATATGA GAAAGAGAGC 
CAGAAAGCAG ATGAGTACCT GCGGGAGATC CAGGAACTGG GCCAGCTGAT CCAGGCTGTG 
CAGCAGTGCA TTGAGGCTGC AGGACATGAG CACCAGCCAG ACATGCAAAA GAGTCTGCTC 
AGGGTTGGCC TGGAGGGGCT TAGGGGAGGG TGTGTGGGAA CTGGGGTGCG CTGGGCAGCC 
AGGGAAGTTC ATTTCCATTT GGTTCTTTTT AGGCGGCTTC ATTTGGAAAG TGTTTCCTTG 
ACAGATTTCC ACCTGACAGC TTCGTGCACA TGTGTCAGGA CCTTCGAGTG CTCAATGCTA 
TTAGAGACTA CCACATTGGG ATCCCTCTCA CCTACACCCA GTATCCCTAT GTATGGCATG 
AGGGTGTTTA CAACTGGGCA TGGCAGGATA AGGGGCAGGA GATGTGGTCT GCAGATCCCT 
GGCAAACAGG CCTTATATCA ACTGATCCTT AACCAAGGAA AACACAGATA CAAGCAGCTC 
ACCATCCAGG TGCTGCTAGA CAGGCTTGTG TTGCGGAGGC TTTATCCCCT GGCTATCCAG 
ATATGCGAGT ACCTGCGCCT TCCTGAAGTC CAGGGTGTCA GCAGGATCCT AGCCCACTGG 
GCCTGCTACA AGGTGCAGCA GAAAGATGTC TCAGATGAAG ATGTAGCACG GGCTATTAAT 
CAGAAGCTGG GTGACACGCC TGGTGTTTCA TACTCTGACA TTGCTGCGAG GGCCTATGGC 
TGTGGCCGCA CTGAACTGGC CATTAAGCTG CTGGAGTATG AGCCACGCTC AGGGGAGCAG 
GTACCTCTTC TCCTAAAGAT GAAGAGGAGC AAACTGGCAC TGAGCAAGGC TATTGAGAGT 
GGAGACACTG ACCTGGTGTT TACAGTGTTG CTGCACCTAA AGAATGAACT CAACCGAGGA 
GACTTTTTCA TGACTCTCCG GAACCAACCC ATGGCCCTGA GTTTGTATCG ACAGTTCTGT 
AAGCATCAGG AGCTAGACAC TCTGAAGGAC CTTTACAATC AGGACGACAA TCACCAGGAG 
TTGGGCAGCT TCCACATCAG AGCCAGCTAT GCCGCTGAAG AGCGTATTGA AGGGCGAGTG 
GCAGCTCTGC AGACGGCAGC TGATGCCTTC TACAAAGCCA AGAATGAATT TGCAGCCAAG 
GCCACAGAGG ATCAAATGAG GCTCCTGCGG ATACAGCGAC GCCTAGAGGA TGAGCTAGGG 
GGCAGGTTCC TAGACCTATC TCTACACGAC ACTGTCACCA CTCTTATCCT TGGAGGCCAC 
AACAAGCGGG CAGAGCAGCT GGCACGTGAC TTTCGCATCC CTGATAAGAG ACTATGGTGG 
TTAAAGCTGG CTGCCCTGGC AGATTTGGAA GATTGGGAGG AGCTGGAGAA GTTTTCCAAA 
AGCAAGAAAT CACCGATTGG TTACCTGCCC TTTGTAGAGA TCTGCATGAA ACAGCATAAC 
AAACATGAGG CCAAGAAGTA TGCTTCCCGT GTGGGCCCTG AACAAAAGGT CAAGGCTTTG 
CTTCTTGTTG GGGATGTGGC TCAGGCTGCA GAGGTGGCTA TTGAGCACCG GAATGAAACA 
GAGCTGAGCC TCGTTTTGTC CCACTGCACT GGAGCCACAG ATGGGGCCAT AGCTGACAAG 
ATTCAGCGGG CCAGGGCACA AGCCCAGAAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174832.1
CDS	76..2811
Translation	MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELKDCLVAAAPYGGPIALLRNCWRKEKAASVRPVLEIYSASGLPLASLLWKSGPVVALGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAYRFTLSANVGDLKLRRMPEVPGLQSAPSCWTTLCHDRVPHILLAVGPDLYLLDHATCSAVTPAGLAPGVSSFLQMAVSFTYRYLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGNAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPVASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLIQAVQQCIEAAGHEHQPDMQKSLLRVGLEGLRGGCVGTGVRWAAREVHFHLVLFRRLHLESVSLTDFHLTASCTCVRTFECSMLLETTTLGSLSPTPSIPMYGMRVFTTGHGRIRGRRCGLQIPGKQALYQLILNQGKHRYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELDTLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRIQRRLEDELGGRFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLAALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKHEAKKYASRVGPEQKVKALLLVGDVAQAAEVAIEHRNETELSLVLSHCTGATDGAIADKIQRARAQAQKK

Target ORF information:

RefSeq Version	XM_017319343.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319343.1

ORF Insert Sequence:

ATGGACTGTT ACACTGCGAA CTGGAATCCA CTCGGGGACT CTGCCTTTTA CAGGAAGTAT 
GAGCTGTACA GCATGGACTG GGACCTGAAG GAGGAGCTCA AGGATTGCCT GGTGGCTGCT 
GCACCCTATG GGGGTCCCAT TGCTCTACTG AGGAACTGTT GGAGAAAAGA GAAAGCTGCC 
AGCGTGCGGC CAGTACTGGA GATCTACTCT GCATCTGGCT TACCACTGGC TAGCCTGCTG 
TGGAAGAGTG GGCCTGTGGT GGCCCTCGGC TGGTCAGCTG AGGAGGAGCT GCTCTGTGTG 
CAAGAAGATG GTGCAGTGCT AGTTTATGGC CTTCATGGAG ACTTCCGGAG GCACTTCAGC 
ATGGGCAATG AAGTGCTTCA GAACCGTGTC CTGGATGCCC GGATTTTTCA CACTGAGTTT 
GGTTCAGGGG TGGCCATCCT TACAGGAGCC TATCGCTTTA CTCTGAGTGC CAATGTGGGT 
GACCTCAAAC TCCGTCGGAT GCCAGAGGTG CCAGGTCTGC AAAGTGCACC CTCATGTTGG 
ACCACACTGT GCCACGACCG AGTACCACAC ATTCTTCTGG CTGTAGGACC CGATCTTTAC 
CTTCTGGATC ATGCTACCTG CTCTGCAGTG ACACCTGCTG GCTTAGCCCC AGGAGTGAGC 
AGCTTCCTAC AGATGGCTGT ATCCTTCACG TATCGTTACC TGGCACTCTT CACAGACACA 
GGCTACATCT GGATGGGGAC AGCATCACTC AAGGAGAAGC TGTGTGAGTT CAACTGCAAC 
ATCCGGGCTC CCCCGAAGCA GATGGTCTGG TGTAGCCGTC CTCGGAGCAA GGAAAGGGCT 
GTTGTTGTGG CCTGGGAGAG GCGGCTGATG GTGGTGGGCA ATGCGCCTGA AAGCATCCAG 
TTTGTGCTCG ATGAGGACTC CTACTTAGTG CCTGAGCTTG ATGGGGTCCG CATCTTCTCC 
CGAAGCACCC ATGAGTTCCT GCATGAAGTC CCAGTGGCCA GTGAAGAGAT CTTCAAAATT 
GCCTCAATGG CCCCTGGAGC ACTGCTGTTA GAGGCCCAGA AGGAATATGA GAAAGAGAGC 
CAGAAAGCAG ATGAGTACCT GCGGGAGATC CAGGAACTGG GCCAGCTGAT CCAGGCTGTG 
CAGCAGTGCA TTGAGGCTGC AGGACATGAG CACCAGCCAG ACATGCAAAA GAGTCTGCTC 
AGGGTTGGCC TGGAGGGGCT TAGGGGAGGG TGTGTGGGAA CTGGGGTGCG CTGGGCAGCC 
AGGGAAGTTC ATTTCCATTT GGTTCTTTTT AGGCGGCTTC ATTTGGAAAG TGTTTCCTTG 
ACAGATTTCC ACCTGACAGC TTCGTGCACA TGTGTCAGGA CCTTCGAGTG CTCAATGCTA 
TTAGAGACTA CCACATTGGG ATCCCTCTCA CCTACACCCA GTATCCCTAT GTATGGCATG 
AGGGTGTTTA CAACTGGGCA TGGCAGGATA AGGGGCAGGA GATGTGGTCT GCAGATCCCT 
GGCAAACAGG CCTTATATCA ACTGATCCTT AACCAAGGAA AACACAGATA CAAGCAGCTC 
ACCATCCAGG TGCTGCTAGA CAGGCTTGTG TTGCGGAGGC TTTATCCCCT GGCTATCCAG 
ATATGCGAGT ACCTGCGCCT TCCTGAAGTC CAGGGTGTCA GCAGGATCCT AGCCCACTGG 
GCCTGCTACA AGGTGCAGCA GAAAGATGTC TCAGATGAAG ATGTAGCACG GGCTATTAAT 
CAGAAGCTGG GTGACACGCC TGGTGTTTCA TACTCTGACA TTGCTGCGAG GGCCTATGGC 
TGTGGCCGCA CTGAACTGGC CATTAAGCTG CTGGAGTATG AGCCACGCTC AGGGGAGCAG 
GTACCTCTTC TCCTAAAGAT GAAGAGGAGC AAACTGGCAC TGAGCAAGGC TATTGAGAGT 
GGAGACACTG ACCTGGTGTT TACAGTGTTG CTGCACCTAA AGAATGAACT CAACCGAGGA 
GACTTTTTCA TGACTCTCCG GAACCAACCC ATGGCCCTGA GTTTGTATCG ACAGTTCTGT 
AAGCATCAGG AGCTAGACAC TCTGAAGGAC CTTTACAATC AGGACGACAA TCACCAGGAG 
TTGGGCAGCT TCCACATCAG AGCCAGCTAT GCCGCTGAAG AGCGTATTGA AGGGCGAGTG 
GCAGCTCTGC AGACGGCAGC TGATGCCTTC TACAAAGCCA AGAATGAATT TGCAGCCAAG 
GCCACAGAGG ATCAAATGAG GCTCCTGCGG ATACAGCGAC GCCTAGAGGA TGAGCTAGGG 
GGCAGGTTCC TAGACCTATC TCTACACGAC ACTGTCACCA CTCTTATCCT TGGAGGCCAC 
AACAAGCGGG CAGAGCAGCT GGCACGTGAC TTTCGCATCC CTGATAAGAG ACTATGGTGG 
TTAAAGCTGG CTGCCCTGGC AGATTTGGAA GATTGGGAGG AGCTGGAGAA GTTTTCCAAA 
AGCAAGAAAT CACCGATTGG TTACCTGCCC TTTGTAGAGA TCTGCATGAA ACAGCATAAC 
AAACATGAGG CCAAGAAGTA TGCTTCCCGT GTGGGCCCTG AACAAAAGGT CAAGGCTTTG 
CTTCTTGTTG GGGATGTGGC TCAGGCTGCA GAGGTGGCTA TTGAGCACCG GAATGAAACA 
GAGCTGAGCC TCGTTTTGTC CCACTGCACT GGAGCCACAG ATGGGGCCAT AGCTGACAAG 
ATTCAGCGGG CCAGGGCACA AGCCCAGAAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps16 ( XM_017319344.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174833.1 Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu80474
Clone ID Related Accession (Same CDS sequence)	XM_017319344.1 , NM_001362651.1
Accession Version	XM_017319344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 16 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG GCAATGCGCC TGAAAGCATC CAGTTTGTGC TCGATGAGGA CTCCTACTTA 
GTGCCTGAGC TTGATGGGGT CCGCATCTTC TCCCGAAGCA CCCATGAGTT CCTGCATGAA 
GTCCCAGTGG CCAGTGAAGA GATCTTCAAA ATTGCCTCAA TGGCCCCTGG AGCACTGCTG 
TTAGAGGCCC AGAAGGAATA TGAGAAAGAG AGCCAGAAAG CAGATGAGTA CCTGCGGGAG 
ATCCAGGAAC TGGGCCAGCT GATCCAGGCT GTGCAGCAGT GCATTGAGGC TGCAGGACAT 
GAGCACCAGC CAGACATGCA AAAGAGTCTG CTCAGGGCGG CTTCATTTGG AAAGTGTTTC 
CTTGACAGAT TTCCACCTGA CAGCTTCGTG CACATGTGTC AGGACCTTCG AGTGCTCAAT 
GCTATTAGAG ACTACCACAT TGGGATCCCT CTCACCTACA CCCAATACAA GCAGCTCACC 
ATCCAGGTGC TGCTAGACAG GCTTGTGTTG CGGAGGCTTT ATCCCCTGGC TATCCAGATA 
TGCGAGTACC TGCGCCTTCC TGAAGTCCAG GGTGTCAGCA GGATCCTAGC CCACTGGGCC 
TGCTACAAGG TGCAGCAGAA AGATGTCTCA GATGAAGATG TAGCACGGGC TATTAATCAG 
AAGCTGGGTG ACACGCCTGG TGTTTCATAC TCTGACATTG CTGCGAGGGC CTATGGCTGT 
GGCCGCACTG AACTGGCCAT TAAGCTGCTG GAGTATGAGC CACGCTCAGG GGAGCAGGTA 
CCTCTTCTCC TAAAGATGAA GAGGAGCAAA CTGGCACTGA GCAAGGCTAT TGAGAGTGGA 
GACACTGACC TGGTGTTGCT GCACCTAAAG AATGAACTCA ACCGAGGAGA CTTTTTCATG 
ACTCTCCGGA ACCAACCCAT GGCCCTGAGT TTGTATCGAC AGTTCTGTAA GCATCAGGAG 
CTAGACACTC TGAAGGACCT TTACAATCAG GACGACAATC ACCAGGAGTT GGGCAGCTTC 
CACATCAGAG CCAGCTATGC CGCTGAAGAG CGTATTGAAG GGCGAGTGGC AGCTCTGCAG 
ACGGCAGCTG ATGCCTTCTA CAAAGCCAAG AATGAATTTG CAGCCAAGGC CACAGAGGAT 
CAAATGAGGC TCCTGCGGAT ACAGCGACGC CTAGAGGATG AGCTAGGGGG CAGGTTCCTA 
GACCTATCTC TACACGACAC TGTCACCACT CTTATCCTTG GAGGCCACAA CAAGCGGGCA 
GAGCAGCTGG CACGTGACTT TCGCATCCCT GATAAGAGAC TATGGTGGTT AAAGCTGGCT 
GCCCTGGCAG ATTTGGAAGA TTGGGAGGAG CTGGAGAAGT TTTCCAAAAG CAAGAAATCA 
CCGATTGGTT ACCTGCCCTT TGTAGAGATC TGCATGAAAC AGCATAACAA ACATGAGGCC 
AAGAAGTATG CTTCCCGTGT GGGCCCTGAA CAAAAGGTCA AGGCTTTGCT TCTTGTTGGG 
GATGTGGCTC AGGCTGCAGA GGTGGCTATT GAGCACCGGA ATGAAACAGA GCTGAGCCTC 
GTTTTGTCCC ACTGCACTGG AGCCACAGAT GGGGCCATAG CTGACAAGAT TCAGCGGGCC 
AGGGCACAAG CCCAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174833.1
CDS	818..2461
Translation	MVVGNAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPVASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLIQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAIRDYHIGIPLTYTQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELDTLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRIQRRLEDELGGRFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLAALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKHEAKKYASRVGPEQKVKALLLVGDVAQAAEVAIEHRNETELSLVLSHCTGATDGAIADKIQRARAQAQKK

Target ORF information:

RefSeq Version	XM_017319344.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319344.1

ORF Insert Sequence:

ATGGTGGTGG GCAATGCGCC TGAAAGCATC CAGTTTGTGC TCGATGAGGA CTCCTACTTA 
GTGCCTGAGC TTGATGGGGT CCGCATCTTC TCCCGAAGCA CCCATGAGTT CCTGCATGAA 
GTCCCAGTGG CCAGTGAAGA GATCTTCAAA ATTGCCTCAA TGGCCCCTGG AGCACTGCTG 
TTAGAGGCCC AGAAGGAATA TGAGAAAGAG AGCCAGAAAG CAGATGAGTA CCTGCGGGAG 
ATCCAGGAAC TGGGCCAGCT GATCCAGGCT GTGCAGCAGT GCATTGAGGC TGCAGGACAT 
GAGCACCAGC CAGACATGCA AAAGAGTCTG CTCAGGGCGG CTTCATTTGG AAAGTGTTTC 
CTTGACAGAT TTCCACCTGA CAGCTTCGTG CACATGTGTC AGGACCTTCG AGTGCTCAAT 
GCTATTAGAG ACTACCACAT TGGGATCCCT CTCACCTACA CCCAATACAA GCAGCTCACC 
ATCCAGGTGC TGCTAGACAG GCTTGTGTTG CGGAGGCTTT ATCCCCTGGC TATCCAGATA 
TGCGAGTACC TGCGCCTTCC TGAAGTCCAG GGTGTCAGCA GGATCCTAGC CCACTGGGCC 
TGCTACAAGG TGCAGCAGAA AGATGTCTCA GATGAAGATG TAGCACGGGC TATTAATCAG 
AAGCTGGGTG ACACGCCTGG TGTTTCATAC TCTGACATTG CTGCGAGGGC CTATGGCTGT 
GGCCGCACTG AACTGGCCAT TAAGCTGCTG GAGTATGAGC CACGCTCAGG GGAGCAGGTA 
CCTCTTCTCC TAAAGATGAA GAGGAGCAAA CTGGCACTGA GCAAGGCTAT TGAGAGTGGA 
GACACTGACC TGGTGTTGCT GCACCTAAAG AATGAACTCA ACCGAGGAGA CTTTTTCATG 
ACTCTCCGGA ACCAACCCAT GGCCCTGAGT TTGTATCGAC AGTTCTGTAA GCATCAGGAG 
CTAGACACTC TGAAGGACCT TTACAATCAG GACGACAATC ACCAGGAGTT GGGCAGCTTC 
CACATCAGAG CCAGCTATGC CGCTGAAGAG CGTATTGAAG GGCGAGTGGC AGCTCTGCAG 
ACGGCAGCTG ATGCCTTCTA CAAAGCCAAG AATGAATTTG CAGCCAAGGC CACAGAGGAT 
CAAATGAGGC TCCTGCGGAT ACAGCGACGC CTAGAGGATG AGCTAGGGGG CAGGTTCCTA 
GACCTATCTC TACACGACAC TGTCACCACT CTTATCCTTG GAGGCCACAA CAAGCGGGCA 
GAGCAGCTGG CACGTGACTT TCGCATCCCT GATAAGAGAC TATGGTGGTT AAAGCTGGCT 
GCCCTGGCAG ATTTGGAAGA TTGGGAGGAG CTGGAGAAGT TTTCCAAAAG CAAGAAATCA 
CCGATTGGTT ACCTGCCCTT TGTAGAGATC TGCATGAAAC AGCATAACAA ACATGAGGCC 
AAGAAGTATG CTTCCCGTGT GGGCCCTGAA CAAAAGGTCA AGGCTTTGCT TCTTGTTGGG 
GATGTGGCTC AGGCTGCAGA GGTGGCTATT GAGCACCGGA ATGAAACAGA GCTGAGCCTC 
GTTTTGTCCC ACTGCACTGG AGCCACAGAT GGGGCCATAG CTGACAAGAT TCAGCGGGCC 
AGGGCACAAG CCCAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Homozygous mutation of VPS16 gene is responsible for an autosomal recessive adolescent-onset primary dystonia.
Scientific reports625834(2016 May)
Cai X,Chen X,Wu S,Liu W,Zhang X,Zhang D,He S,Wang B,Zhang M,Zhang Y,Li Z,Luo K,Cai Z,Li W

Mammalian CORVET is required for fusion and conversion of distinct early endosome subpopulations.
Traffic (Copenhagen, Denmark)15(12)1366-89(2014 Dec)
Perini ED,Schaefer R,St?ter M,Kalaidzidis Y,Zerial M

UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs.
Proceedings of the National Academy of Sciences of the United States of America111(7)2716-21(2014 Feb)
Pirooz SD,He S,Zhang T,Zhang X,Zhao Z,Oh S,O'Connell D,Khalilzadeh P,Amini-Bavil-Olyaee S,Farzan M,Liang C

Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.
Molecular biology of the cell22(10)1699-715(2011 May)
Zlatic SA,Tornieri K,L'Hernault SW,Faundez V

SPE-39 family proteins interact with the HOPS complex and function in lysosomal delivery.
Molecular biology of the cell20(4)1223-40(2009 Feb)
Zhu GD,Salazar G,Zlatic SA,Fiza B,Doucette MM,Heilman CJ,Levey AI,Faundez V,L'hernault SW

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Vps16 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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