PRODH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRODH
Entrez Gene ID	5625
Full Name	proline dehydrogenase 1
Synonyms	HSPOX2,PIG6,POX,PRODH1,PRODH2,TP53I6
General protein information	Preferred Names proline dehydrogenase 1 Names proline dehydrogenase 1, mitochondrial p53-induced gene 6 protein proline dehydrogenase (oxidase) 1 proline oxidase 2 proline oxidase, mitochondrial tumor protein p53 inducible protein 6
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q11.21
Summary	This gene encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in this gene are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). This gene is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010].
Disorder MIM:	606810
Disorder Html:	Hyperprolinemia, type I, 239500 (3); {Schizophrenia, susceptibility to, 4}, 600850 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_016335.5	NP_057419.5	proline dehydrogenase 1, mitochondrial isoform 1 precursor
NM_016335.4	NP_057419.4	proline dehydrogenase 1, mitochondrial isoform 1 precursor
NM_001195226.1	NP_001182155.1	proline dehydrogenase 1, mitochondrial isoform 2
NM_001195226.2	NP_001182155.2	proline dehydrogenase 1, mitochondrial isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	LOC102724788	XP_006724998.1
Homo sapiens (human)	PRODH	NP_057419.4
Xenopus tropicalis (tropical clawed frog)	LOC100494891	XP_002932121.2
Danio rerio (zebrafish)	LOC100537991	XP_003198647.2
	LOC695506	XP_001082216.1
Canis lupus familiaris (dog)	PRODH	XP_005636623.1
Bos taurus (cattle)	PRODH	NP_001068653.1
Mus musculus (house mouse)	Prodh	NP_035302.2
Rattus norvegicus (Norway rat)	Prodh	NP_001129250.1
Drosophila melanogaster (fruit fly)	slgA	NP_996527.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003860	XP_310420.5
Caenorhabditis elegans (roundworm)	CELE_B0513.5	NP_502669.2
Pan troglodytes (chimpanzee)	PRODH	XP_525525.2
Gallus gallus (chicken)	PRODH	XP_001234062.3

Gene Ontology
Bibliography

Related articles in PubMed

Collagen-derived proline promotes pancreatic ductal adenocarcinoma cell survival under nutrient limited conditions.
Olivares O, Mayers JR, Gouirand V, Torrence ME, Gicquel T, Borge L, Lac S, Roques J, Lavaut MN, Berthez?ne P, Rubis M, Secq V, Garcia S, Moutardier V, Lombardo D, Iovanna JL, Tomasini R, Guillaumond F, Vander Heiden MG, Vasseur S
Nature communications816031(2017 Jul)

Proline dehydrogenase promotes senescence through the generation of reactive oxygen species.
Nagano T, Nakashima A, Onishi K, Kawai K, Awai Y, Kinugasa M, Iwasaki T, Kikkawa U, Kamada S
Journal of cell science130(8)1413-1420(2017 04)

Functional Consequences of Intracellular Proline Levels Manipulation Affecting PRODH/POX-Dependent Pro-Apoptotic Pathways in a Novel in Vitro Cell Culture Model.
Zareba I, Surazynski A, Chrusciel M, Miltyk W, Doroszko M, Rahman N, Palka J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology43(2)670-684(2017)

Cytosolic Accumulation of L-Proline Disrupts GABA-Ergic Transmission through GAD Blockade.
Crabtree GW, Park AJ, Gordon JA, Gogos JA
Cell reports17(2)570-582(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that PRODH plays a causative role in DNA damage-induced senescence through the enzymatic generation of reactive oxygen species
Title: Proline dehydrogenase promotes senescence through the generation of reactive oxygen species.

PRODH/POX knockdown decreased DNA and collagen biosynthesis, whereas increased prolidase activity and intracellular proline level in MCF-7shPRODH/POX cells.
Title: Functional Consequences of Intracellular Proline Levels Manipulation Affecting PRODH/POX-Dependent Pro-Apoptotic Pathways in a Novel in Vitro Cell Culture Model.

Here, we show that Prodh-deficient mice with elevated CNS L-proline display specific deficits in high-frequency GABA-ergic transmission and gamma-band oscillations. We find that L-proline is a GABA-mimetic and can act at multiple GABA-ergic targets
Title: Cytosolic Accumulation of L-Proline Disrupts GABA-Ergic Transmission through GAD Blockade.

the frequency of a recurrent small 22q11.2 deletion encompassing PRODH and the neighboring DGCR6 gene in three case-control studies, was studied.
Title: The 22q11 PRODH/DGCR6 deletion is frequent in hyperprolinemic subjects but is not a strong risk factor for ASD.

The findings support a major role for the PRODH 757TT, 1766GG, and 1852AA genotypes alone and in combination for schizophrenia susceptibility.
Title: Relationship between polymorphisms in the proline dehydrogenase gene and schizophrenia risk.

The following PRODH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRODH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27345	NM_016335.4 Latest version!	Homo sapiens proline dehydrogenase 1 (PRODH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$249.50-$349.30 $499.00
OHu27404	NM_001195226.1 Latest version!	Homo sapiens proline dehydrogenase 1 (PRODH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu107959	NM_001195226.2 Latest version!	Homo sapiens proline dehydrogenase 1 (PRODH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu107955	NM_016335.5 Latest version!	Homo sapiens proline dehydrogenase 1 (PRODH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRODH ( NM_016335.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057419.4 Homo sapiens proline dehydrogenase 1 (PRODH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27345
Clone ID Related Accession (Same CDS sequence)	NM_016335.4
Accession Version	NM_016335.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	proline dehydrogenase 1, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U79754.1, CN362765.1, BC118597.1, CB216963.1, BC121809.1, BC036534.1 and BU730461.1. This sequence is a reference standard in the RefSeqGene project. On Aug 28, 2010 this sequence version replaced NM_016335.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U79754.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTGA GGCGCGCCCT GCCCGCGCTG CGCCCCTGCA TTCCCCGCTT CGTCCCGCTG 
TCCACGGCGC CGGCCTCCCG CGAGCAGCCC GCAGCGGGCC CAGCGGCCGT GCCAGGAGGT 
GGGTCGGCCA CGGCAGTGCG GCCGCCGGTG CCCGCCGTGG ACTTCGGCAA CGCGCAGGAG 
GCGTACCGCA GCCGGCGAAC CTGGGAGCTG GCGCGGAGCC TGCTGGTGCT GCGCTTGTGC 
GCCTGGCCCG CGCTGCTGGC GCGCCACGAG CAGCTGCTGT ATGTTTCCAG GAAACTTCTA 
GGACAGAGGC TATTCAACAA GCTCATGAAG ATGACCTTCT ATGGGCATTT TGTAGCCGGG 
GAGGACCAGG AGTCCATCCA GCCCCTGCTT CGGCACTACA GGGCCTTCGG TGTCAGCGCC 
ATCCTGGACT ATGGAGTGGA GGAGGACCTG AGCCCCGAGG AGGCAGAGCA CAAGGAGATG 
GAGTCCTGCA CCTCAGCTGC GGAGAGGGAT GGCAGTGGCA CGAATAAGCG GGACAAGCAA 
TACCAGGCCC ACCGGGCCTT CGGGGACCGC AGGAATGGTG TCATCAGTGC CCGCACCTAC 
TTCTACGCCA ATGAGGCCAA GTGCGACAGC CACATGGAGA CATTCTTGCG CTGCATCGAA 
GCCTCAGGTA GAGTCAGCGA TGACGGCTTC ATAGCCATTA AGCTCACAGC ACTGGGGAGA 
CCCCAGTTTC TGCTGCAGTT CTCAGAGGTG CTGGCCAAGT GGAGGTGCTT CTTTCACCAA 
ATGGCTGTGG AGCAAGGGCA GGCGGGCCTG GCTGCCATGG ACACCAAGCT GGAGGTGGCG 
GTGCTGCAGG AAAGTGTCGC AAAGTTGGGC ATCGCATCCA GGGCTGAGAT TGAGGACTGG 
TTCACGGCAG AGACCCTGGG AGTGTCTGGC ACCATGGACC TGCTGGACTG GAGCAGCCTC 
ATCGACAGCA GGACCAAGCT GTCCAAGCAC TTGGTAGTCC CCAACGCACA GACAGGACAG 
CTGGAGCCCC TGCTGTCCCG GTTCACTGAG GAGGAGGAGC TACAGATGAC CAGGATGCTA 
CAGCGGATGG ATGTCCTGGC CAAGAAAGCC ACAGAGATGG GCGTGCGGCT GATGGTGGAT 
GCCGAGCAGA CCTACTTCCA GCCGGCCATC AGCCGCCTGA CGCTGGAGAT GCAGCGGAAG 
TTCAATGTGG AGAAGCCGCT CATCTTCAAC ACATACCAGT GCTACCTCAA GGATGCCTAT 
GACAATGTGA CCCTGGACGT GGAGCTGGCT CGCCGTGAGG GCTGGTGTTT TGGGGCCAAG 
CTGGTGCGGG GCGCATACCT GGCCCAGGAG CGAGCCCGTG CGGCAGAGAT CGGCTATGAG 
GACCCCATCA ACCCCACGTA CGAGGCCACC AACGCCATGT ACCACAGGTG CCTGGACTAC 
GTGTTGGAGG AGCTGAAGCA CAACGCCAAG GCCAAGGTGA TGGTGGCCTC CCACAATGAG 
GACACAGTGC GCTTTGCACT GCGCAGGATG GAGGAGCTGG GCCTGCATCC TGCTGACCAC 
CAGGTGTACT TTGGACAGCT GCTAGGCATG TGTGACCAGA TCAGCTTCCC GCTGGGCCAG 
GCCGGCTACC CCGTGTACAA GTACGTGCCC TATGGCCCCG TGATGGAGGT GCTGCCCTAC 
TTGTCCCGCC GTGCCCTGGA GAACAGCAGC CTCATGAAGG GCACCCATCG GGAGCGGCAG 
CTGCTGTGGC TGGAGCTCTT GAGGCGGCTC CGAACTGGCA ACCTCTTCCA TCGCCCTGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057419.4
CDS	205..2007
Translation	MALRRALPALRPCIPRFVPLSTAPASREQPAAGPAAVPGGGSATAVRPPVPAVDFGNAQEAYRSRRTWELARSLLVLRLCAWPALLARHEQLLYVSRKLLGQRLFNKLMKMTFYGHFVAGEDQESIQPLLRHYRAFGVSAILDYGVEEDLSPEEAEHKEMESCTSAAERDGSGTNKRDKQYQAHRAFGDRRNGVISARTYFYANEAKCDSHMETFLRCIEASGRVSDDGFIAIKLTALGRPQFLLQFSEVLAKWRCFFHQMAVEQGQAGLAAMDTKLEVAVLQESVAKLGIASRAEIEDWFTAETLGVSGTMDLLDWSSLIDSRTKLSKHLVVPNAQTGQLEPLLSRFTEEEELQMTRMLQRMDVLAKKATEMGVRLMVDAEQTYFQPAISRLTLEMQRKFNVEKPLIFNTYQCYLKDAYDNVTLDVELARREGWCFGAKLVRGAYLAQERARAAEIGYEDPINPTYEATNAMYHRCLDYVLEELKHNAKAKVMVASHNEDTVRFALRRMEELGLHPADHQVYFGQLLGMCDQISFPLGQAGYPVYKYVPYGPVMEVLPYLSRRALENSSLMKGTHRERQLLWLELLRRLRTGNLFHRPA

Target ORF information:

RefSeq Version	NM_016335.4
Organism	Homo sapiens(human)
Definition	Homo sapiens proline dehydrogenase 1 (PRODH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016335.4

ORF Insert Sequence:

ATGGCTCTGA GGCGCGCCCT GCCCGCGCTG CGCCCCTGCA TTCCCCGCTT CGTCCCGCTG 
TCCACGGCGC CGGCCTCCCG CGAGCAGCCC GCAGCGGGCC CAGCGGCCGT GCCAGGAGGT 
GGGTCGGCCA CGGCAGTGCG GCCGCCGGTG CCCGCCGTGG ACTTCGGCAA CGCGCAGGAG 
GCGTACCGCA GCCGGCGAAC CTGGGAGCTG GCGCGGAGCC TGCTGGTGCT GCGCTTGTGC 
GCCTGGCCCG CGCTGCTGGC GCGCCACGAG CAGCTGCTGT ATGTTTCCAG GAAACTTCTA 
GGACAGAGGC TATTCAACAA GCTCATGAAG ATGACCTTCT ATGGGCATTT TGTAGCCGGG 
GAGGACCAGG AGTCCATCCA GCCCCTGCTT CGGCACTACA GGGCCTTCGG TGTCAGCGCC 
ATCCTGGACT ATGGAGTGGA GGAGGACCTG AGCCCCGAGG AGGCAGAGCA CAAGGAGATG 
GAGTCCTGCA CCTCAGCTGC GGAGAGGGAT GGCAGTGGCA CGAATAAGCG GGACAAGCAA 
TACCAGGCCC ACCGGGCCTT CGGGGACCGC AGGAATGGTG TCATCAGTGC CCGCACCTAC 
TTCTACGCCA ATGAGGCCAA GTGCGACAGC CACATGGAGA CATTCTTGCG CTGCATCGAA 
GCCTCAGGTA GAGTCAGCGA TGACGGCTTC ATAGCCATTA AGCTCACAGC ACTGGGGAGA 
CCCCAGTTTC TGCTGCAGTT CTCAGAGGTG CTGGCCAAGT GGAGGTGCTT CTTTCACCAA 
ATGGCTGTGG AGCAAGGGCA GGCGGGCCTG GCTGCCATGG ACACCAAGCT GGAGGTGGCG 
GTGCTGCAGG AAAGTGTCGC AAAGTTGGGC ATCGCATCCA GGGCTGAGAT TGAGGACTGG 
TTCACGGCAG AGACCCTGGG AGTGTCTGGC ACCATGGACC TGCTGGACTG GAGCAGCCTC 
ATCGACAGCA GGACCAAGCT GTCCAAGCAC TTGGTAGTCC CCAACGCACA GACAGGACAG 
CTGGAGCCCC TGCTGTCCCG GTTCACTGAG GAGGAGGAGC TACAGATGAC CAGGATGCTA 
CAGCGGATGG ATGTCCTGGC CAAGAAAGCC ACAGAGATGG GCGTGCGGCT GATGGTGGAT 
GCCGAGCAGA CCTACTTCCA GCCGGCCATC AGCCGCCTGA CGCTGGAGAT GCAGCGGAAG 
TTCAATGTGG AGAAGCCGCT CATCTTCAAC ACATACCAGT GCTACCTCAA GGATGCCTAT 
GACAATGTGA CCCTGGACGT GGAGCTGGCT CGCCGTGAGG GCTGGTGTTT TGGGGCCAAG 
CTGGTGCGGG GCGCATACCT GGCCCAGGAG CGAGCCCGTG CGGCAGAGAT CGGCTATGAG 
GACCCCATCA ACCCCACGTA CGAGGCCACC AACGCCATGT ACCACAGGTG CCTGGACTAC 
GTGTTGGAGG AGCTGAAGCA CAACGCCAAG GCCAAGGTGA TGGTGGCCTC CCACAATGAG 
GACACAGTGC GCTTTGCACT GCGCAGGATG GAGGAGCTGG GCCTGCATCC TGCTGACCAC 
CAGGTGTACT TTGGACAGCT GCTAGGCATG TGTGACCAGA TCAGCTTCCC GCTGGGCCAG 
GCCGGCTACC CCGTGTACAA GTACGTGCCC TATGGCCCCG TGATGGAGGT GCTGCCCTAC 
TTGTCCCGCC GTGCCCTGGA GAACAGCAGC CTCATGAAGG GCACCCATCG GGAGCGGCAG 
CTGCTGTGGC TGGAGCTCTT GAGGCGGCTC CGAACTGGCA ACCTCTTCCA TCGCCCTGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRODH ( NM_001195226.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182155.1 Homo sapiens proline dehydrogenase 1 (PRODH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27404
Clone ID Related Accession (Same CDS sequence)	NM_001195226.1
Accession Version	NM_001195226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	proline dehydrogenase 1, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC121809.1, CB216963.1, BC118597.1, BC036534.1 and BU730461.1. Transcript Variant: This variant (2) is missing an internal exon at the 5' end compared to variant 1, resulting in translation initiation from an in-frame downstream AUG and an isoform (2) with a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC118597.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGATGA CCTTCTATGG GCATTTTGTA GCCGGGGAGG ACCAGGAGTC CATCCAGCCC 
CTGCTTCGGC ACTACAGGGC CTTCGGTGTC AGCGCCATCC TGGACTATGG AGTGGAGGAG 
GACCTGAGCC CCGAGGAGGC AGAGCACAAG GAGATGGAGT CCTGCACCTC AGCTGCGGAG 
AGGGATGGCA GTGGCACGAA TAAGCGGGAC AAGCAATACC AGGCCCACCG GGCCTTCGGG 
GACCGCAGGA ATGGTGTCAT CAGTGCCCGC ACCTACTTCT ACGCCAATGA GGCCAAGTGC 
GACAGCCACA TGGAGACATT CTTGCGCTGC ATCGAAGCCT CAGGTAGAGT CAGCGATGAC 
GGCTTCATAG CCATTAAGCT CACAGCACTG GGGAGACCCC AGTTTCTGCT GCAGTTCTCA 
GAGGTGCTGG CCAAGTGGAG GTGCTTCTTT CACCAAATGG CTGTGGAGCA AGGGCAGGCG 
GGCCTGGCTG CCATGGACAC CAAGCTGGAG GTGGCGGTGC TGCAGGAAAG TGTCGCAAAG 
TTGGGCATCG CATCCAGGGC TGAGATTGAG GACTGGTTCA CGGCAGAGAC CCTGGGAGTG 
TCTGGCACCA TGGACCTGCT GGACTGGAGC AGCCTCATCG ACAGCAGGAC CAAGCTGTCC 
AAGCACTTGG TAGTCCCCAA CGCACAGACA GGACAGCTGG AGCCCCTGCT GTCCCGGTTC 
ACTGAGGAGG AGGAGCTACA GATGACCAGG ATGCTACAGC GGATGGATGT CCTGGCCAAG 
AAAGCCACAG AGATGGGCGT GCGGCTGATG GTGGATGCCG AGCAGACCTA CTTCCAGCCG 
GCCATCAGCC GCCTGACGCT GGAGATGCAG CGGAAGTTCA ATGTGGAGAA GCCGCTCATC 
TTCAACACAT ACCAGTGCTA CCTCAAGGAT GCCTATGACA ATGTGACCCT GGACGTGGAG 
CTGGCTCGCC GTGAGGGCTG GTGTTTTGGG GCCAAGCTGG TGCGGGGCGC ATACCTGGCC 
CAGGAGCGAG CCCGTGCGGC AGAGATCGGC TATGAGGACC CCATCAACCC CACGTACGAG 
GCCACCAACG CCATGTACCA CAGGTGCCTG GACTACGTGT TGGAGGAGCT GAAGCACAAC 
GCCAAGGCCA AGGTGATGGT GGCCTCCCAC AATGAGGACA CAGTGCGCTT TGCACTGCGC 
AGGATGGAGG AGCTGGGCCT GCATCCTGCT GACCACCAGG TGTACTTTGG ACAGCTGCTA 
GGCATGTGTG ACCAGATCAG CTTCCCGCTG GGCCAGGCCG GCTACCCCGT GTACAAGTAC 
GTGCCCTATG GCCCCGTGAT GGAGGTGCTG CCCTACTTGT CCCGCCGTGC CCTGGAGAAC 
AGCAGCCTCA TGAAGGGCAC CCATCGGGAG CGGCAGCTGC TGTGGCTGGA GCTCTTGAGG 
CGGCTCCGAA CTGGCAACCT CTTCCATCGC CCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182155.1
CDS	114..1592
Translation	MKMTFYGHFVAGEDQESIQPLLRHYRAFGVSAILDYGVEEDLSPEEAEHKEMESCTSAAERDGSGTNKRDKQYQAHRAFGDRRNGVISARTYFYANEAKCDSHMETFLRCIEASGRVSDDGFIAIKLTALGRPQFLLQFSEVLAKWRCFFHQMAVEQGQAGLAAMDTKLEVAVLQESVAKLGIASRAEIEDWFTAETLGVSGTMDLLDWSSLIDSRTKLSKHLVVPNAQTGQLEPLLSRFTEEEELQMTRMLQRMDVLAKKATEMGVRLMVDAEQTYFQPAISRLTLEMQRKFNVEKPLIFNTYQCYLKDAYDNVTLDVELARREGWCFGAKLVRGAYLAQERARAAEIGYEDPINPTYEATNAMYHRCLDYVLEELKHNAKAKVMVASHNEDTVRFALRRMEELGLHPADHQVYFGQLLGMCDQISFPLGQAGYPVYKYVPYGPVMEVLPYLSRRALENSSLMKGTHRERQLLWLELLRRLRTGNLFHRPA

Target ORF information:

RefSeq Version	NM_001195226.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proline dehydrogenase 1 (PRODH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195226.1

ORF Insert Sequence:

ATGAAGATGA CCTTCTATGG GCATTTTGTA GCCGGGGAGG ACCAGGAGTC CATCCAGCCC 
CTGCTTCGGC ACTACAGGGC CTTCGGTGTC AGCGCCATCC TGGACTATGG AGTGGAGGAG 
GACCTGAGCC CCGAGGAGGC AGAGCACAAG GAGATGGAGT CCTGCACCTC AGCTGCGGAG 
AGGGATGGCA GTGGCACGAA TAAGCGGGAC AAGCAATACC AGGCCCACCG GGCCTTCGGG 
GACCGCAGGA ATGGTGTCAT CAGTGCCCGC ACCTACTTCT ACGCCAATGA GGCCAAGTGC 
GACAGCCACA TGGAGACATT CTTGCGCTGC ATCGAAGCCT CAGGTAGAGT CAGCGATGAC 
GGCTTCATAG CCATTAAGCT CACAGCACTG GGGAGACCCC AGTTTCTGCT GCAGTTCTCA 
GAGGTGCTGG CCAAGTGGAG GTGCTTCTTT CACCAAATGG CTGTGGAGCA AGGGCAGGCG 
GGCCTGGCTG CCATGGACAC CAAGCTGGAG GTGGCGGTGC TGCAGGAAAG TGTCGCAAAG 
TTGGGCATCG CATCCAGGGC TGAGATTGAG GACTGGTTCA CGGCAGAGAC CCTGGGAGTG 
TCTGGCACCA TGGACCTGCT GGACTGGAGC AGCCTCATCG ACAGCAGGAC CAAGCTGTCC 
AAGCACTTGG TAGTCCCCAA CGCACAGACA GGACAGCTGG AGCCCCTGCT GTCCCGGTTC 
ACTGAGGAGG AGGAGCTACA GATGACCAGG ATGCTACAGC GGATGGATGT CCTGGCCAAG 
AAAGCCACAG AGATGGGCGT GCGGCTGATG GTGGATGCCG AGCAGACCTA CTTCCAGCCG 
GCCATCAGCC GCCTGACGCT GGAGATGCAG CGGAAGTTCA ATGTGGAGAA GCCGCTCATC 
TTCAACACAT ACCAGTGCTA CCTCAAGGAT GCCTATGACA ATGTGACCCT GGACGTGGAG 
CTGGCTCGCC GTGAGGGCTG GTGTTTTGGG GCCAAGCTGG TGCGGGGCGC ATACCTGGCC 
CAGGAGCGAG CCCGTGCGGC AGAGATCGGC TATGAGGACC CCATCAACCC CACGTACGAG 
GCCACCAACG CCATGTACCA CAGGTGCCTG GACTACGTGT TGGAGGAGCT GAAGCACAAC 
GCCAAGGCCA AGGTGATGGT GGCCTCCCAC AATGAGGACA CAGTGCGCTT TGCACTGCGC 
AGGATGGAGG AGCTGGGCCT GCATCCTGCT GACCACCAGG TGTACTTTGG ACAGCTGCTA 
GGCATGTGTG ACCAGATCAG CTTCCCGCTG GGCCAGGCCG GCTACCCCGT GTACAAGTAC 
GTGCCCTATG GCCCCGTGAT GGAGGTGCTG CCCTACTTGT CCCGCCGTGC CCTGGAGAAC 
AGCAGCCTCA TGAAGGGCAC CCATCGGGAG CGGCAGCTGC TGTGGCTGGA GCTCTTGAGG 
CGGCTCCGAA CTGGCAACCT CTTCCATCGC CCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRODH ( NM_001195226.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182155.2 Homo sapiens proline dehydrogenase 1 (PRODH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu107959
Clone ID Related Accession (Same CDS sequence)	NM_001195226.2
Accession Version	NM_001195226.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	proline dehydrogenase 1, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007326.28. On Jan 31, 2019 this sequence version replaced NM_001195226.1. Transcript Variant: This variant (2) is missing an internal exon at the 5' end compared to variant 1, resulting in translation initiation from an in-frame downstream AUG and an isoform (2) with a shorter N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC118597.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGATGA CCTTCTATGG GCATTTTGTA GCCGGGGAGG ACCAGGAGTC CATCCAGCCC 
CTGCTTCGGC ACTACAGGGC CTTCGGTGTC AGCGCCATCC TGGACTATGG AGTGGAGGAG 
GACCTGAGCC CCGAGGAGGC AGAGCACAAG GAGATGGAGT CCTGCACCTC AGCTGCGGAG 
AGGGATGGCA GTGGCACGAA TAAGCGGGAC AAGCAATACC AGGCCCACTG GGCCTTCGGG 
GACCGCAGGA ATGGTGTCAT CAGTGCCCGC ACCTACTTCT ACGCCAATGA GGCCAAGTGC 
GACAGCCACA TGGAGACATT CTTGCGCTGC ATCGAAGCCT CAGGTAGAGT CAGCGATGAC 
GGCTTCATAG CCATTAAGCT CACAGCACTG GGGAGACCCC AGTTTCTGCT GCAGTTCTCA 
GAGGTGCTGG CCAAGTGGAG GTGCTTCTTT CACCAAATGG CTGTGGAGCA AGGGCAGGCG 
GGCCTGGCTG CCATGGACAC CAAGCTGGAG GTGGCGGTGC TGCAGGAAAG TGTCGCAAAG 
TTGGGCATCG CATCCAGGGC TGAGATTGAG GACTGGTTCA CGGCAGAGAC CCTGGGAGTG 
TCTGGCACCA TGGACCTGCT GGACTGGAGC AGCCTCATCG ACAGCAGGAC CAAGCTGTCC 
AAGCACTTGG TAGTCCCCAA CGCACAGACA GGACAGCTGG AGCCCCTGCT GTCCCGGTTC 
ACTGAGGAGG AGGAGCTACA GATGACCAGG ATGCTACAGC GGATGGATGT CCTGGCCAAG 
AAAGCCACAG AGATGGGCGT GCGGCTGATG GTGGATGCCG AGCAGACCTA CTTCCAGCCG 
GCCATCAGCC GCCTGACGCT GGAGATGCAG CGGAAGTTCA ATGTGGAGAA GCCGCTCATC 
TTCAACACAT ACCAGTGCTA CCTCAAGGAT GCCTATGACA ATGTGACCCT GGACGTGGAG 
CTGGCTCGCC GTGAGGGCTG GTGTTTTGGG GCCAAGCTGG TGCGGGGCGC ATACCTGGCC 
CAGGAGCGAG CCCGTGCGGC AGAGATCGGC TATGAGGACC CCATCAACCC CACGTACGAG 
GCCACCAACG CCATGTACCA CAGGTGCCTG GACTACGTGT TGGAGGAGCT GAAGCACAAC 
GCCAAGGCCA AGGTGATGGT GGCCTCCCAC AATGAGGACA CAGTGCGCTT TGCACTGCGC 
AGGATGGAGG AGCTGGGCCT GCATCCTGCT GACCACCGGG TGTACTTTGG ACAGCTGCTA 
GGCATGTGTG ACCAGATCAG CTTCCCGCTG GGCCAGGCCG GCTACCCCGT GTACAAGTAC 
GTGCCCTATG GCCCCGTGAT GGAGGTGCTG CCCTACTTGT CCCGCCGTGC CCTGGAGAAC 
AGCAGCCTCA TGAAGGGCAC CCATCGGGAG CGGCAGCTGC TGTGGCTGGA GCTCTTGAGG 
CGGCTCCGAA CTGGCAACCT CTTCCATCGC CCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182155.2
CDS	216..1694
Translation	MKMTFYGHFVAGEDQESIQPLLRHYRAFGVSAILDYGVEEDLSPEEAEHKEMESCTSAAERDGSGTNKRDKQYQAHWAFGDRRNGVISARTYFYANEAKCDSHMETFLRCIEASGRVSDDGFIAIKLTALGRPQFLLQFSEVLAKWRCFFHQMAVEQGQAGLAAMDTKLEVAVLQESVAKLGIASRAEIEDWFTAETLGVSGTMDLLDWSSLIDSRTKLSKHLVVPNAQTGQLEPLLSRFTEEEELQMTRMLQRMDVLAKKATEMGVRLMVDAEQTYFQPAISRLTLEMQRKFNVEKPLIFNTYQCYLKDAYDNVTLDVELARREGWCFGAKLVRGAYLAQERARAAEIGYEDPINPTYEATNAMYHRCLDYVLEELKHNAKAKVMVASHNEDTVRFALRRMEELGLHPADHRVYFGQLLGMCDQISFPLGQAGYPVYKYVPYGPVMEVLPYLSRRALENSSLMKGTHRERQLLWLELLRRLRTGNLFHRPA

Target ORF information:

RefSeq Version	NM_001195226.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proline dehydrogenase 1 (PRODH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195226.2

ORF Insert Sequence:

ATGAAGATGA CCTTCTATGG GCATTTTGTA GCCGGGGAGG ACCAGGAGTC CATCCAGCCC 
CTGCTTCGGC ACTACAGGGC CTTCGGTGTC AGCGCCATCC TGGACTATGG AGTGGAGGAG 
GACCTGAGCC CCGAGGAGGC AGAGCACAAG GAGATGGAGT CCTGCACCTC AGCTGCGGAG 
AGGGATGGCA GTGGCACGAA TAAGCGGGAC AAGCAATACC AGGCCCACTG GGCCTTCGGG 
GACCGCAGGA ATGGTGTCAT CAGTGCCCGC ACCTACTTCT ACGCCAATGA GGCCAAGTGC 
GACAGCCACA TGGAGACATT CTTGCGCTGC ATCGAAGCCT CAGGTAGAGT CAGCGATGAC 
GGCTTCATAG CCATTAAGCT CACAGCACTG GGGAGACCCC AGTTTCTGCT GCAGTTCTCA 
GAGGTGCTGG CCAAGTGGAG GTGCTTCTTT CACCAAATGG CTGTGGAGCA AGGGCAGGCG 
GGCCTGGCTG CCATGGACAC CAAGCTGGAG GTGGCGGTGC TGCAGGAAAG TGTCGCAAAG 
TTGGGCATCG CATCCAGGGC TGAGATTGAG GACTGGTTCA CGGCAGAGAC CCTGGGAGTG 
TCTGGCACCA TGGACCTGCT GGACTGGAGC AGCCTCATCG ACAGCAGGAC CAAGCTGTCC 
AAGCACTTGG TAGTCCCCAA CGCACAGACA GGACAGCTGG AGCCCCTGCT GTCCCGGTTC 
ACTGAGGAGG AGGAGCTACA GATGACCAGG ATGCTACAGC GGATGGATGT CCTGGCCAAG 
AAAGCCACAG AGATGGGCGT GCGGCTGATG GTGGATGCCG AGCAGACCTA CTTCCAGCCG 
GCCATCAGCC GCCTGACGCT GGAGATGCAG CGGAAGTTCA ATGTGGAGAA GCCGCTCATC 
TTCAACACAT ACCAGTGCTA CCTCAAGGAT GCCTATGACA ATGTGACCCT GGACGTGGAG 
CTGGCTCGCC GTGAGGGCTG GTGTTTTGGG GCCAAGCTGG TGCGGGGCGC ATACCTGGCC 
CAGGAGCGAG CCCGTGCGGC AGAGATCGGC TATGAGGACC CCATCAACCC CACGTACGAG 
GCCACCAACG CCATGTACCA CAGGTGCCTG GACTACGTGT TGGAGGAGCT GAAGCACAAC 
GCCAAGGCCA AGGTGATGGT GGCCTCCCAC AATGAGGACA CAGTGCGCTT TGCACTGCGC 
AGGATGGAGG AGCTGGGCCT GCATCCTGCT GACCACCGGG TGTACTTTGG ACAGCTGCTA 
GGCATGTGTG ACCAGATCAG CTTCCCGCTG GGCCAGGCCG GCTACCCCGT GTACAAGTAC 
GTGCCCTATG GCCCCGTGAT GGAGGTGCTG CCCTACTTGT CCCGCCGTGC CCTGGAGAAC 
AGCAGCCTCA TGAAGGGCAC CCATCGGGAG CGGCAGCTGC TGTGGCTGGA GCTCTTGAGG 
CGGCTCCGAA CTGGCAACCT CTTCCATCGC CCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRODH ( NM_016335.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057419.5 Homo sapiens proline dehydrogenase 1 (PRODH), transcript variant 1, mRNA.

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CloneID	OHu107955
Clone ID Related Accession (Same CDS sequence)	NM_016335.5
Accession Version	NM_016335.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	proline dehydrogenase 1, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007326.28. This sequence is a reference standard in the RefSeqGene project. On Jan 31, 2019 this sequence version replaced NM_016335.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1803614.19175.1, SRR1660805.167443.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTGA GGCGCGCCCT GCCCGCGCTG CGCCCCTGCA TTCCCCGCTT CGTCCCGCTG 
TCCACGGCGC CGGCCTCCCG CGAGCAGCCC GCAGCGGGCC CAGCGGCCGT GCCAGGAGGT 
GGGTCGGCCA CGGCAGTGCG GCCGCCGGTG CCCGCCGTGG ACTTCGGCAA CGCGCAGGAG 
GCGTACCGCA GCCGGCGAAC CTGGGAGCTG GCGCGGAGCC TGCTGGTGCT GCGCTTGTGC 
GCCTGGCCCG CGCTGCTGGC GCGCCACGAG CAGCTGCTGT ATGTTTCCAG GAAACTTCTA 
GGACAGAGGC TATTCAACAA GCTCATGAAG ATGACCTTCT ATGGGCATTT TGTAGCCGGG 
GAGGACCAGG AGTCCATCCA GCCCCTGCTT CGGCACTACA GGGCCTTCGG TGTCAGCGCC 
ATCCTGGACT ATGGAGTGGA GGAGGACCTG AGCCCCGAGG AGGCAGAGCA CAAGGAGATG 
GAGTCCTGCA CCTCAGCTGC GGAGAGGGAT GGCAGTGGCA CGAATAAGCG GGACAAGCAA 
TACCAGGCCC ACTGGGCCTT CGGGGACCGC AGGAATGGTG TCATCAGTGC CCGCACCTAC 
TTCTACGCCA ATGAGGCCAA GTGCGACAGC CACATGGAGA CATTCTTGCG CTGCATCGAA 
GCCTCAGGTA GAGTCAGCGA TGACGGCTTC ATAGCCATTA AGCTCACAGC ACTGGGGAGA 
CCCCAGTTTC TGCTGCAGTT CTCAGAGGTG CTGGCCAAGT GGAGGTGCTT CTTTCACCAA 
ATGGCTGTGG AGCAAGGGCA GGCGGGCCTG GCTGCCATGG ACACCAAGCT GGAGGTGGCG 
GTGCTGCAGG AAAGTGTCGC AAAGTTGGGC ATCGCATCCA GGGCTGAGAT TGAGGACTGG 
TTCACGGCAG AGACCCTGGG AGTGTCTGGC ACCATGGACC TGCTGGACTG GAGCAGCCTC 
ATCGACAGCA GGACCAAGCT GTCCAAGCAC TTGGTAGTCC CCAACGCACA GACAGGACAG 
CTGGAGCCCC TGCTGTCCCG GTTCACTGAG GAGGAGGAGC TACAGATGAC CAGGATGCTA 
CAGCGGATGG ATGTCCTGGC CAAGAAAGCC ACAGAGATGG GCGTGCGGCT GATGGTGGAT 
GCCGAGCAGA CCTACTTCCA GCCGGCCATC AGCCGCCTGA CGCTGGAGAT GCAGCGGAAG 
TTCAATGTGG AGAAGCCGCT CATCTTCAAC ACATACCAGT GCTACCTCAA GGATGCCTAT 
GACAATGTGA CCCTGGACGT GGAGCTGGCT CGCCGTGAGG GCTGGTGTTT TGGGGCCAAG 
CTGGTGCGGG GCGCATACCT GGCCCAGGAG CGAGCCCGTG CGGCAGAGAT CGGCTATGAG 
GACCCCATCA ACCCCACGTA CGAGGCCACC AACGCCATGT ACCACAGGTG CCTGGACTAC 
GTGTTGGAGG AGCTGAAGCA CAACGCCAAG GCCAAGGTGA TGGTGGCCTC CCACAATGAG 
GACACAGTGC GCTTTGCACT GCGCAGGATG GAGGAGCTGG GCCTGCATCC TGCTGACCAC 
CGGGTGTACT TTGGACAGCT GCTAGGCATG TGTGACCAGA TCAGCTTCCC GCTGGGCCAG 
GCCGGCTACC CCGTGTACAA GTACGTGCCC TATGGCCCCG TGATGGAGGT GCTGCCCTAC 
TTGTCCCGCC GTGCCCTGGA GAACAGCAGC CTCATGAAGG GCACCCATCG GGAGCGGCAG 
CTGCTGTGGC TGGAGCTCTT GAGGCGGCTC CGAACTGGCA ACCTCTTCCA TCGCCCTGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057419.5
CDS	205..2007
Translation	MALRRALPALRPCIPRFVPLSTAPASREQPAAGPAAVPGGGSATAVRPPVPAVDFGNAQEAYRSRRTWELARSLLVLRLCAWPALLARHEQLLYVSRKLLGQRLFNKLMKMTFYGHFVAGEDQESIQPLLRHYRAFGVSAILDYGVEEDLSPEEAEHKEMESCTSAAERDGSGTNKRDKQYQAHWAFGDRRNGVISARTYFYANEAKCDSHMETFLRCIEASGRVSDDGFIAIKLTALGRPQFLLQFSEVLAKWRCFFHQMAVEQGQAGLAAMDTKLEVAVLQESVAKLGIASRAEIEDWFTAETLGVSGTMDLLDWSSLIDSRTKLSKHLVVPNAQTGQLEPLLSRFTEEEELQMTRMLQRMDVLAKKATEMGVRLMVDAEQTYFQPAISRLTLEMQRKFNVEKPLIFNTYQCYLKDAYDNVTLDVELARREGWCFGAKLVRGAYLAQERARAAEIGYEDPINPTYEATNAMYHRCLDYVLEELKHNAKAKVMVASHNEDTVRFALRRMEELGLHPADHRVYFGQLLGMCDQISFPLGQAGYPVYKYVPYGPVMEVLPYLSRRALENSSLMKGTHRERQLLWLELLRRLRTGNLFHRPA

Target ORF information:

RefSeq Version	NM_016335.5
Organism	Homo sapiens(human)
Definition	Homo sapiens proline dehydrogenase 1 (PRODH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016335.5

ORF Insert Sequence:

ATGGCTCTGA GGCGCGCCCT GCCCGCGCTG CGCCCCTGCA TTCCCCGCTT CGTCCCGCTG 
TCCACGGCGC CGGCCTCCCG CGAGCAGCCC GCAGCGGGCC CAGCGGCCGT GCCAGGAGGT 
GGGTCGGCCA CGGCAGTGCG GCCGCCGGTG CCCGCCGTGG ACTTCGGCAA CGCGCAGGAG 
GCGTACCGCA GCCGGCGAAC CTGGGAGCTG GCGCGGAGCC TGCTGGTGCT GCGCTTGTGC 
GCCTGGCCCG CGCTGCTGGC GCGCCACGAG CAGCTGCTGT ATGTTTCCAG GAAACTTCTA 
GGACAGAGGC TATTCAACAA GCTCATGAAG ATGACCTTCT ATGGGCATTT TGTAGCCGGG 
GAGGACCAGG AGTCCATCCA GCCCCTGCTT CGGCACTACA GGGCCTTCGG TGTCAGCGCC 
ATCCTGGACT ATGGAGTGGA GGAGGACCTG AGCCCCGAGG AGGCAGAGCA CAAGGAGATG 
GAGTCCTGCA CCTCAGCTGC GGAGAGGGAT GGCAGTGGCA CGAATAAGCG GGACAAGCAA 
TACCAGGCCC ACTGGGCCTT CGGGGACCGC AGGAATGGTG TCATCAGTGC CCGCACCTAC 
TTCTACGCCA ATGAGGCCAA GTGCGACAGC CACATGGAGA CATTCTTGCG CTGCATCGAA 
GCCTCAGGTA GAGTCAGCGA TGACGGCTTC ATAGCCATTA AGCTCACAGC ACTGGGGAGA 
CCCCAGTTTC TGCTGCAGTT CTCAGAGGTG CTGGCCAAGT GGAGGTGCTT CTTTCACCAA 
ATGGCTGTGG AGCAAGGGCA GGCGGGCCTG GCTGCCATGG ACACCAAGCT GGAGGTGGCG 
GTGCTGCAGG AAAGTGTCGC AAAGTTGGGC ATCGCATCCA GGGCTGAGAT TGAGGACTGG 
TTCACGGCAG AGACCCTGGG AGTGTCTGGC ACCATGGACC TGCTGGACTG GAGCAGCCTC 
ATCGACAGCA GGACCAAGCT GTCCAAGCAC TTGGTAGTCC CCAACGCACA GACAGGACAG 
CTGGAGCCCC TGCTGTCCCG GTTCACTGAG GAGGAGGAGC TACAGATGAC CAGGATGCTA 
CAGCGGATGG ATGTCCTGGC CAAGAAAGCC ACAGAGATGG GCGTGCGGCT GATGGTGGAT 
GCCGAGCAGA CCTACTTCCA GCCGGCCATC AGCCGCCTGA CGCTGGAGAT GCAGCGGAAG 
TTCAATGTGG AGAAGCCGCT CATCTTCAAC ACATACCAGT GCTACCTCAA GGATGCCTAT 
GACAATGTGA CCCTGGACGT GGAGCTGGCT CGCCGTGAGG GCTGGTGTTT TGGGGCCAAG 
CTGGTGCGGG GCGCATACCT GGCCCAGGAG CGAGCCCGTG CGGCAGAGAT CGGCTATGAG 
GACCCCATCA ACCCCACGTA CGAGGCCACC AACGCCATGT ACCACAGGTG CCTGGACTAC 
GTGTTGGAGG AGCTGAAGCA CAACGCCAAG GCCAAGGTGA TGGTGGCCTC CCACAATGAG 
GACACAGTGC GCTTTGCACT GCGCAGGATG GAGGAGCTGG GCCTGCATCC TGCTGACCAC 
CGGGTGTACT TTGGACAGCT GCTAGGCATG TGTGACCAGA TCAGCTTCCC GCTGGGCCAG 
GCCGGCTACC CCGTGTACAA GTACGTGCCC TATGGCCCCG TGATGGAGGT GCTGCCCTAC 
TTGTCCCGCC GTGCCCTGGA GAACAGCAGC CTCATGAAGG GCACCCATCG GGAGCGGCAG 
CTGCTGTGGC TGGAGCTCTT GAGGCGGCTC CGAACTGGCA ACCTCTTCCA TCGCCCTGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Collagen-derived proline promotes pancreatic ductal adenocarcinoma cell survival under nutrient limited conditions.
Nature communications816031(2017 Jul)
Olivares O,Mayers JR,Gouirand V,Torrence ME,Gicquel T,Borge L,Lac S,Roques J,Lavaut MN,Berthez?ne P,Rubis M,Secq V,Garcia S,Moutardier V,Lombardo D,Iovanna JL,Tomasini R,Guillaumond F,Vander Heiden MG,Vasseur S

Proline dehydrogenase promotes senescence through the generation of reactive oxygen species.
Journal of cell science130(8)1413-1420(2017 04)
Nagano T,Nakashima A,Onishi K,Kawai K,Awai Y,Kinugasa M,Iwasaki T,Kikkawa U,Kamada S

Functional Consequences of Intracellular Proline Levels Manipulation Affecting PRODH/POX-Dependent Pro-Apoptotic Pathways in a Novel in Vitro Cell Culture Model.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology43(2)670-684(2017)
Zareba I,Surazynski A,Chrusciel M,Miltyk W,Doroszko M,Rahman N,Palka J

Cytosolic Accumulation of L-Proline Disrupts GABA-Ergic Transmission through GAD Blockade.
Cell reports17(2)570-582(2016 10)
Crabtree GW,Park AJ,Gordon JA,Gogos JA

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PRODH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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