Prodh1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prodh1
Entrez Gene ID	680409
Full Name	proline dehydrogenase 1
Synonyms	Prodh
General protein information	Preferred Names proline dehydrogenase 1 Names proline dehydrogenase 1, mitochondrial proline dehydrogenase (oxidase) 1 proline dehydrogenase (proline oxidase) proline dehydrogenase, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q23
Summary	predicted to catalyze the conversion of L-proline, an acceptor and H2O to (S)-1-pyrroline-5-carboxylate and a reduced acceptor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001135778.1	NP_001129250.1	proline dehydrogenase, mitochondrial
XM_006248643.2	XP_006248705.1	proline dehydrogenase 1, mitochondrial isoform X2
XM_006248642.3	XP_006248704.3	proline dehydrogenase 1, mitochondrial isoform X1
XM_008768903.2	XP_008767125.1	proline dehydrogenase 1, mitochondrial isoform X3
NM_001135778.2	NP_001129250.2	proline dehydrogenase 1, mitochondrial

Pathways from BioSystems

Homologies

Homo sapiens (human)	LOC102724788	XP_006724998.1
Homo sapiens (human)	PRODH	NP_057419.4
Xenopus tropicalis (tropical clawed frog)	LOC100494891	XP_002932121.2
Danio rerio (zebrafish)	LOC100537991	XP_003198647.2
	LOC695506	XP_001082216.1
Canis lupus familiaris (dog)	PRODH	XP_005636623.1
Bos taurus (cattle)	PRODH	NP_001068653.1
Mus musculus (house mouse)	Prodh	NP_035302.2
Rattus norvegicus (Norway rat)	Prodh	NP_001129250.1
Drosophila melanogaster (fruit fly)	slgA	NP_996527.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003860	XP_310420.5
Caenorhabditis elegans (roundworm)	CELE_B0513.5	NP_502669.2
Pan troglodytes (chimpanzee)	PRODH	XP_525525.2
Gallus gallus (chicken)	PRODH	XP_001234062.3

Gene Ontology
Bibliography

Related articles in PubMed

DJ-1 cooperates with PYCR1 in cell protection against oxidative stress.
Yasuda T, Kaji Y, Agatsuma T, Niki T, Arisawa M, Shuto S, Ariga H, Iguchi-Ariga SM
Biochemical and biophysical research communications436(2)289-94(2013 Jun)

Mitochondrial localization and function of a subset of 22q11 deletion syndrome candidate genes.
Maynard TM, Meechan DW, Dudevoir ML, Gopalakrishna D, Peters AZ, Heindel CC, Sugimoto TJ, Wu Y, Lieberman JA, Lamantia AS
Molecular and cellular neurosciences39(3)439-51(2008 Nov)

Functional consequences of PRODH missense mutations.
Bender HU, Almashanu S, Steel G, Hu CA, Lin WW, Willis A, Pulver A, Valle D
American journal of human genetics76(3)409-20(2005 Mar)

Proteomic analysis of the mouse liver mitochondrial inner membrane.
Da Cruz S, Xenarios I, Langridge J, Vilbois F, Parone PA, Martinou JC
The Journal of biological chemistry278(42)41566-71(2003 Oct)

The severe form of type I hyperprolinaemia results from homozygous inactivation of the PRODH gene.
Jacquet H, Berthelot J, Bonnemains C, Simard G, Saugier-Veber P, Raux G, Campion D, Bonneau D, Frebourg T
Journal of medical genetics40(1)e7(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prodh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prodh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa39827	NM_001135778.1 Latest version!	Rattus norvegicus proline dehydrogenase (oxidase) 1 (Prodh), nuclear gene encoding mitochondrial protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa34467	XM_006248643.2 Latest version!	Rattus norvegicus proline dehydrogenase 1 (Prodh1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa49788	XM_008768903.2 Latest version!	Rattus norvegicus proline dehydrogenase 1 (Prodh1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa63279	NM_001135778.2 Latest version!	Rattus norvegicus proline dehydrogenase 1 (Prodh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa57721	XM_006248642.3 Latest version!	Rattus norvegicus proline dehydrogenase 1 (Prodh1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prodh1 ( NM_001135778.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001129250.1 Rattus norvegicus proline dehydrogenase (oxidase) 1 (Prodh), nuclear gene encoding mitochondrial protein, mRNA.

Price & Availability↑

CloneID	ORa39827
Clone ID Related Accession (Same CDS sequence)	NM_001135778.1
Accession Version	NM_001135778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2012-02-07
Organism	Rattus norvegicus(Norway rat)
Product	proline dehydrogenase, mitochondrial
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR03078814.1. On Oct 11, 2008 this sequence version replaced XM_001058939.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGCTCTGA AGCGGGTCTT TTTGCTGCGC TCGGTGGCAC CACGCGTTGC TGCCCTCTCA 
ACCAAACCGC GAGCCCAGGA GCAGCCTCCC GCGAACCCTG AGGCTTTGCG GGGATGTGGA 
GCGGCTGAGG CTGTGCGGCC GCCTGTGCCA GCCGTGGACT TCACCAACAC ACAGGAGGCG 
TACCGCAGCC GTCGAAGCTG GGAACTGGCG CGCAACCTGC TAGTGCTGCG GCTGTGTGCG 
TCGCCGGTGC TGCTAGCGCA CCATGAGCAG TTGTTCCAAG TTGCCAGGAA GCTTCTGGGG 
CAGAGGATGT TCGAGAGACT GATGAAGATG ACCTTCTACG GCCATTTCGT GGCTGGCGAG 
GACCAGGAGT CTATCAGGCC TCTGATTCGG CACAACAAAG CCTTTGGTGT TGGCTTTATC 
CTGGACTATG GTGTGGAGGA AGATCTGAGC CCCGAGGAGG CGGAGCGCAA AGAAATGGAG 
TCATGCACCT CTGAAGCTGA AAGAGATGGC AGTGGAGCAA ATAAGAGGGA GAAGCAGTAT 
CAGGTCCACC CCGCCTTCGG AGACCGCAGA GATGGCGTCA TCAGTGCCCG CACTTACTTC 
TATGCCAGCG AAGCCAAATG TGACAACTAC ATGGAGAACT TACTGCAGTG CATCGAGGCC 
TCAGGCGGAG CCAGTGACGG TGGTTTTTCA GCCATTAAGC TCACGGCACT GGGGAGACCA 
CAGTTTCTGC TGCAGTTCTC AGACGTGCTG ACCAGGTGGA GACAGTTTTT TCATCAAATG 
GCTGCAGAGC AGGGACAGGC TGGGCGTCCT GCTGTGGACA CGAAGCTGGA GGTGGCGGTG 
CTCCAGGAGA GCATCGCCAA GATGGGCATC TCGTCCAGGG CTGAGATCGA GAGGTGGTTC 
ACACCAGAGA CGCTGGGAGT GTCTGGCACT GTGGACCTAC TGGACTGGAA CAGCCTCATT 
GACAGCAGGA CCCAGCTCTC CAGGCACCTG GTGGTCCCCA ATGTACAGAC TGGCCAGCTG 
GAGCCCCTGC TGTCACGGTT CACTGAGGAG GAAGAGCAGC AGATGAAGAG GATGCTGCAG 
AGGATGGATG TGCTGGCCAA GGTAGCGAGA GAGGCATGCG TGCGGCTGAT GATTGACGCC 
GAGCAGAGCT ACTTCCAGCC GGCCATCAAC CGCCTGACCC TGGAGATGCA GCGCAGGTTC 
AATGTGGATA AGCCGTTCAT CTTCAACACG TTCCAGTGCT ACCTCAAGGA TGCCTATGAC 
AATGTGACCA TAGATATGGA ACTGTCTCGC CGTGAGGGCT GGTGTTTTGG GGCCAAGCTG 
GTACGTGGTG CATACATGGC CCAGGAGCGA GCCAGGGCTG TGGAGATCGG CTACGAGGAC 
CCCATCAACC CTACATATGA AGCCACCAAT GCCATGTACC ACAGGTGCCT TAACTATGTT 
CTGGAGGAGC TGAAGCACAG CACAAAGGCC GAGGTGATGG TGGCTTCCCA CAATGAGGAC 
ACCGTGAGCT TTACGTTGTG CAGGATGAAG GAGTTAGGCC TGCATCCTGC TGATGGTCAG 
GTGTGCTTCG GACAGCTGCT GGGGATGTGT GACCAAATTA GCTTCCCACT AGGCCAGGCA 
GGCTTCCCTG TGTACAAGTA TGTACCCTAC GGGCCTGTGA TGGAGGTGCT CCCGTACCTG 
TCCCGCCGTG CCCTGGAGAA CAGCAGCATC ATGAAGGGTG CTCAGCGAGA GAGGCAGCTG 
CTATGGCAGG AGCTCTGCAG GCGGCTGCGC ACAGGCAGCC TCTTCCACCA TCCGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129250.1
CDS	25..1824
Translation	MALKRVFLLRSVAPRVAALSTKPRAQEQPPANPEALRGCGAAEAVRPPVPAVDFTNTQEAYRSRRSWELARNLLVLRLCASPVLLAHHEQLFQVARKLLGQRMFERLMKMTFYGHFVAGEDQESIRPLIRHNKAFGVGFILDYGVEEDLSPEEAERKEMESCTSEAERDGSGANKREKQYQVHPAFGDRRDGVISARTYFYASEAKCDNYMENLLQCIEASGGASDGGFSAIKLTALGRPQFLLQFSDVLTRWRQFFHQMAAEQGQAGRPAVDTKLEVAVLQESIAKMGISSRAEIERWFTPETLGVSGTVDLLDWNSLIDSRTQLSRHLVVPNVQTGQLEPLLSRFTEEEEQQMKRMLQRMDVLAKVAREACVRLMIDAEQSYFQPAINRLTLEMQRRFNVDKPFIFNTFQCYLKDAYDNVTIDMELSRREGWCFGAKLVRGAYMAQERARAVEIGYEDPINPTYEATNAMYHRCLNYVLEELKHSTKAEVMVASHNEDTVSFTLCRMKELGLHPADGQVCFGQLLGMCDQISFPLGQAGFPVYKYVPYGPVMEVLPYLSRRALENSSIMKGAQRERQLLWQELCRRLRTGSLFHHPA

Target ORF information:

RefSeq Version	NM_001135778.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proline dehydrogenase (oxidase) 1 (Prodh), nuclear gene encoding mitochondrial protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135778.1

ORF Insert Sequence:

ATGGCTCTGA AGCGGGTCTT TTTGCTGCGC TCGGTGGCAC CACGCGTTGC TGCCCTCTCA 
ACCAAACCGC GAGCCCAGGA GCAGCCTCCC GCGAACCCTG AGGCTTTGCG GGGATGTGGA 
GCGGCTGAGG CTGTGCGGCC GCCTGTGCCA GCCGTGGACT TCACCAACAC ACAGGAGGCG 
TACCGCAGCC GTCGAAGCTG GGAACTGGCG CGCAACCTGC TAGTGCTGCG GCTGTGTGCG 
TCGCCGGTGC TGCTAGCGCA CCATGAGCAG TTGTTCCAAG TTGCCAGGAA GCTTCTGGGG 
CAGAGGATGT TCGAGAGACT GATGAAGATG ACCTTCTACG GCCATTTCGT GGCTGGCGAG 
GACCAGGAGT CTATCAGGCC TCTGATTCGG CACAACAAAG CCTTTGGTGT TGGCTTTATC 
CTGGACTATG GTGTGGAGGA AGATCTGAGC CCCGAGGAGG CGGAGCGCAA AGAAATGGAG 
TCATGCACCT CTGAAGCTGA AAGAGATGGC AGTGGAGCAA ATAAGAGGGA GAAGCAGTAT 
CAGGTCCACC CCGCCTTCGG AGACCGCAGA GATGGCGTCA TCAGTGCCCG CACTTACTTC 
TATGCCAGCG AAGCCAAATG TGACAACTAC ATGGAGAACT TACTGCAGTG CATCGAGGCC 
TCAGGCGGAG CCAGTGACGG TGGTTTTTCA GCCATTAAGC TCACGGCACT GGGGAGACCA 
CAGTTTCTGC TGCAGTTCTC AGACGTGCTG ACCAGGTGGA GACAGTTTTT TCATCAAATG 
GCTGCAGAGC AGGGACAGGC TGGGCGTCCT GCTGTGGACA CGAAGCTGGA GGTGGCGGTG 
CTCCAGGAGA GCATCGCCAA GATGGGCATC TCGTCCAGGG CTGAGATCGA GAGGTGGTTC 
ACACCAGAGA CGCTGGGAGT GTCTGGCACT GTGGACCTAC TGGACTGGAA CAGCCTCATT 
GACAGCAGGA CCCAGCTCTC CAGGCACCTG GTGGTCCCCA ATGTACAGAC TGGCCAGCTG 
GAGCCCCTGC TGTCACGGTT CACTGAGGAG GAAGAGCAGC AGATGAAGAG GATGCTGCAG 
AGGATGGATG TGCTGGCCAA GGTAGCGAGA GAGGCATGCG TGCGGCTGAT GATTGACGCC 
GAGCAGAGCT ACTTCCAGCC GGCCATCAAC CGCCTGACCC TGGAGATGCA GCGCAGGTTC 
AATGTGGATA AGCCGTTCAT CTTCAACACG TTCCAGTGCT ACCTCAAGGA TGCCTATGAC 
AATGTGACCA TAGATATGGA ACTGTCTCGC CGTGAGGGCT GGTGTTTTGG GGCCAAGCTG 
GTACGTGGTG CATACATGGC CCAGGAGCGA GCCAGGGCTG TGGAGATCGG CTACGAGGAC 
CCCATCAACC CTACATATGA AGCCACCAAT GCCATGTACC ACAGGTGCCT TAACTATGTT 
CTGGAGGAGC TGAAGCACAG CACAAAGGCC GAGGTGATGG TGGCTTCCCA CAATGAGGAC 
ACCGTGAGCT TTACGTTGTG CAGGATGAAG GAGTTAGGCC TGCATCCTGC TGATGGTCAG 
GTGTGCTTCG GACAGCTGCT GGGGATGTGT GACCAAATTA GCTTCCCACT AGGCCAGGCA 
GGCTTCCCTG TGTACAAGTA TGTACCCTAC GGGCCTGTGA TGGAGGTGCT CCCGTACCTG 
TCCCGCCGTG CCCTGGAGAA CAGCAGCATC ATGAAGGGTG CTCAGCGAGA GAGGCAGCTG 
CTATGGCAGG AGCTCTGCAG GCGGCTGCGC ACAGGCAGCC TCTTCCACCA TCCGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prodh1 ( XM_006248643.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248705.1 Rattus norvegicus proline dehydrogenase 1 (Prodh1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa34467
Clone ID Related Accession (Same CDS sequence)	XM_006248643.2
Accession Version	XM_006248643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	proline dehydrogenase 1, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGA AGTGGTGTCT TGGCTCTAGA GTCTACTTTA AAGCTGCCAG CCCTGCCTGT 
GTGTGTCCAC CATTCTCAGT GACCAGGTTG TTCCAAGTTG CCAGGAAGCT TCTGGGGCAG 
AGGATGTTCG AGAGACTGAT GAAGATGACC TTCTACGGCC ATTTCGTGGC TGGCGAGGAC 
CAGGAGTCTA TCAGGCCTCT GATTCGGCAC AACAAAGCCT TTGGTGTTGG CTTTATCCTG 
GACTATGGTG TGGAGGAAGA TCTGAGCCCC GAGGAGGCGG AGCGCAAAGA AATGGAGTCA 
TGCACCTCTG AAGCTGAAAG AGATGGCAGT GGAGCAAATA AGAGGGAGAA GCAGTATCAG 
GTCCACCCCG CCTTCGGAGA CCGCAGAGAT GGCGTCATCA GTGCCCGCAC TTACTTCTAT 
GCCAGCGAAG CCAAATGTGA CAACTACATG GAGAACTTAC TGCAGTGCAT CGAGGCCTCA 
GGCGGAGCCA GTGACGGTGG TTTTTCAGCC ATTAAGCTCA CGGCACTGGG GAGACCACAG 
TTTCTGCTGC AGTTCTCAGA CGTGCTGACC AGGTGGAGAC AGTTTTTTCA TCAAATGGCT 
GCAGAGCAGG GACAGGCTGG GCGTCCTGCT GTGGACACGA AGCTGGAGGT GGCGGTGCTC 
CAGGAGAGCA TCGCCAAGAT GGGCATCTCG TCCAGGGCTG AGATCGAGAG GTGGTTCACA 
CCAGAGACGC TGGGAGTGTC TGGCACTGTG GACCTACTGG ACTGGAACAG CCTCATTGAC 
AGCAGGACCC AGCTCTCCAG GCACCTGGTG GTCCCCAATG TACAGACTGG CCAGCTGGAG 
CCCCTGCTGT CACGGTTCAC TGAGGAGGAA GAGCAGCAGA TGAAGAGGAT GCTGCAGAGG 
ATGGATGTGC TGGCCAAGAG AGCGAGAGAG GCATGCGTGC GGCTGATGAT TGACGCCGAG 
CAGAGCTACT TCCAGCCGGC CATCAGCCGC CTGACCCTGG AGATGCAGCG CAGGTTCAAT 
GTGGATAAGC CGTTCATCTT CAACACGTTC CAGTGCTACC TCAAGGATGC CTATGACAAT 
GTGACCATAG ATATGGAACT GTCTCGCCGT GAGGGCTGGT GTTTTGGGGC CAAGCTGGTA 
CGTGGTGCAT ACATGGCCCA GGAGCGAGCC AGGGCTGTGG AGATCGGCTA CGAGGACCCC 
ATCAACCCTA CATATGAAGC CACCAATGCC ATGTACCACA GGTGCCTTAA CTATGTTCTG 
GAGGAGCTGA AGCACAGCAC AAAGGCCGAG GTGATGGTGG CTTCCCACAA TGAGGACACC 
GTGAGCTTTA CGTTGTGCAG GATGAAGGAG TTAGGCCTGC ATCCTGCTGA TGGTCAGGTG 
TGCTTCGGAC AGCTGCTGGG GATGTGTGAC CAAATTAGCT TCCCACTAGG CCAGGCAGGC 
TTCCCTGTGT ACAAGTATGT ACCCTACGGG CCTGTGATGG AGGTGCTCCC GTACCTGTCC 
CGCCGTGCCC TGGAGAACAG CAGCATCATG AAGGGTGCTC AGCGAGAGAG GCAGCTGCTA 
TGGCAGGAGC TCTGCAGGCG GCTGCGCACA GGCAGCCTCT TCCACCATCC GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248705.1
CDS	155..1771
Translation	MDLKWCLGSRVYFKAASPACVCPPFSVTRLFQVARKLLGQRMFERLMKMTFYGHFVAGEDQESIRPLIRHNKAFGVGFILDYGVEEDLSPEEAERKEMESCTSEAERDGSGANKREKQYQVHPAFGDRRDGVISARTYFYASEAKCDNYMENLLQCIEASGGASDGGFSAIKLTALGRPQFLLQFSDVLTRWRQFFHQMAAEQGQAGRPAVDTKLEVAVLQESIAKMGISSRAEIERWFTPETLGVSGTVDLLDWNSLIDSRTQLSRHLVVPNVQTGQLEPLLSRFTEEEEQQMKRMLQRMDVLAKRAREACVRLMIDAEQSYFQPAISRLTLEMQRRFNVDKPFIFNTFQCYLKDAYDNVTIDMELSRREGWCFGAKLVRGAYMAQERARAVEIGYEDPINPTYEATNAMYHRCLNYVLEELKHSTKAEVMVASHNEDTVSFTLCRMKELGLHPADGQVCFGQLLGMCDQISFPLGQAGFPVYKYVPYGPVMEVLPYLSRRALENSSIMKGAQRERQLLWQELCRRLRTGSLFHHPA

Target ORF information:

RefSeq Version	XM_006248643.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proline dehydrogenase 1 (Prodh1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248643.2

ORF Insert Sequence:

ATGGATTTGA AGTGGTGTCT TGGCTCTAGA GTCTACTTTA AAGCTGCCAG CCCTGCCTGT 
GTGTGTCCAC CATTCTCAGT GACCAGGTTG TTCCAAGTTG CCAGGAAGCT TCTGGGGCAG 
AGGATGTTCG AGAGACTGAT GAAGATGACC TTCTACGGCC ATTTCGTGGC TGGCGAGGAC 
CAGGAGTCTA TCAGGCCTCT GATTCGGCAC AACAAAGCCT TTGGTGTTGG CTTTATCCTG 
GACTATGGTG TGGAGGAAGA TCTGAGCCCC GAGGAGGCGG AGCGCAAAGA AATGGAGTCA 
TGCACCTCTG AAGCTGAAAG AGATGGCAGT GGAGCAAATA AGAGGGAGAA GCAGTATCAG 
GTCCACCCCG CCTTCGGAGA CCGCAGAGAT GGCGTCATCA GTGCCCGCAC TTACTTCTAT 
GCCAGCGAAG CCAAATGTGA CAACTACATG GAGAACTTAC TGCAGTGCAT CGAGGCCTCA 
GGCGGAGCCA GTGACGGTGG TTTTTCAGCC ATTAAGCTCA CGGCACTGGG GAGACCACAG 
TTTCTGCTGC AGTTCTCAGA CGTGCTGACC AGGTGGAGAC AGTTTTTTCA TCAAATGGCT 
GCAGAGCAGG GACAGGCTGG GCGTCCTGCT GTGGACACGA AGCTGGAGGT GGCGGTGCTC 
CAGGAGAGCA TCGCCAAGAT GGGCATCTCG TCCAGGGCTG AGATCGAGAG GTGGTTCACA 
CCAGAGACGC TGGGAGTGTC TGGCACTGTG GACCTACTGG ACTGGAACAG CCTCATTGAC 
AGCAGGACCC AGCTCTCCAG GCACCTGGTG GTCCCCAATG TACAGACTGG CCAGCTGGAG 
CCCCTGCTGT CACGGTTCAC TGAGGAGGAA GAGCAGCAGA TGAAGAGGAT GCTGCAGAGG 
ATGGATGTGC TGGCCAAGAG AGCGAGAGAG GCATGCGTGC GGCTGATGAT TGACGCCGAG 
CAGAGCTACT TCCAGCCGGC CATCAGCCGC CTGACCCTGG AGATGCAGCG CAGGTTCAAT 
GTGGATAAGC CGTTCATCTT CAACACGTTC CAGTGCTACC TCAAGGATGC CTATGACAAT 
GTGACCATAG ATATGGAACT GTCTCGCCGT GAGGGCTGGT GTTTTGGGGC CAAGCTGGTA 
CGTGGTGCAT ACATGGCCCA GGAGCGAGCC AGGGCTGTGG AGATCGGCTA CGAGGACCCC 
ATCAACCCTA CATATGAAGC CACCAATGCC ATGTACCACA GGTGCCTTAA CTATGTTCTG 
GAGGAGCTGA AGCACAGCAC AAAGGCCGAG GTGATGGTGG CTTCCCACAA TGAGGACACC 
GTGAGCTTTA CGTTGTGCAG GATGAAGGAG TTAGGCCTGC ATCCTGCTGA TGGTCAGGTG 
TGCTTCGGAC AGCTGCTGGG GATGTGTGAC CAAATTAGCT TCCCACTAGG CCAGGCAGGC 
TTCCCTGTGT ACAAGTATGT ACCCTACGGG CCTGTGATGG AGGTGCTCCC GTACCTGTCC 
CGCCGTGCCC TGGAGAACAG CAGCATCATG AAGGGTGCTC AGCGAGAGAG GCAGCTGCTA 
TGGCAGGAGC TCTGCAGGCG GCTGCGCACA GGCAGCCTCT TCCACCATCC GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prodh1 ( XM_008768903.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767125.1 Rattus norvegicus proline dehydrogenase 1 (Prodh1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa49788
Clone ID Related Accession (Same CDS sequence)	XM_008768903.2
Accession Version	XM_008768903.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	proline dehydrogenase 1, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768903.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGAGA GACTGATGAA GATGACCTTC TACGGCCATT TCGTGGCTGG CGAGGACCAG 
GAGTCTATCA GGCCTCTGAT TCGGCACAAC AAAGCCTTTG GTGTTGGCTT TATCCTGGAC 
TATGGTGTGG AGGAAGATCT GAGCCCCGAG GAGGCGGAGC GCAAAGAAAT GGAGTCATGC 
ACCTCTGAAG CTGAAAGAGA TGGCAGTGGA GCAAATAAGA GGGAGAAGCA GTATCAGGTC 
CACCCCGCCT TCGGAGACCG CAGAGATGGC GTCATCAGTG CCCGCACTTA CTTCTATGCC 
AGCGAAGCCA AATGTGACAA CTACATGGAG AACTTACTGC AGTGCATCGA GGCCTCAGGC 
GGAGCCAGTG ACGGTGGTTT TTCAGCCATT AAGCTCACGG CACTGGGGAG ACCACAGTTT 
CTGCTGCAGT TCTCAGACGT GCTGACCAGG TGGAGACAGT TTTTTCATCA AATGGCTGCA 
GAGCAGGGAC AGGCTGGGCG TCCTGCTGTG GACACGAAGC TGGAGGTGGC GGTGCTCCAG 
GAGAGCATCG CCAAGATGGG CATCTCGTCC AGGGCTGAGA TCGAGAGGTG GTTCACACCA 
GAGACGCTGG GAGTGTCTGG CACTGTGGAC CTACTGGACT GGAACAGCCT CATTGACAGC 
AGGACCCAGC TCTCCAGGCA CCTGGTGGTC CCCAATGTAC AGACTGGCCA GCTGGAGCCC 
CTGCTGTCAC GGTTCACTGA GGAGGAAGAG CAGCAGATGA AGAGGATGCT GCAGAGGATG 
GATGTGCTGG CCAAGAGAGC GAGAGAGGCA TGCGTGCGGC TGATGATTGA CGCCGAGCAG 
AGCTACTTCC AGCCGGCCAT CAGCCGCCTG ACCCTGGAGA TGCAGCGCAG GTTCAATGTG 
GATAAGCCGT TCATCTTCAA CACGTTCCAG TGCTACCTCA AGGATGCCTA TGACAATGTG 
ACCATAGATA TGGAACTGTC TCGCCGTGAG GGCTGGTGTT TTGGGGCCAA GCTGGTACGT 
GGTGCATACA TGGCCCAGGA GCGAGCCAGG GCTGTGGAGA TCGGCTACGA GGACCCCATC 
AACCCTACAT ATGAAGCCAC CAATGCCATG TACCACAGGT GCCTTAACTA TGTTCTGGAG 
GAGCTGAAGC ACAGCACAAA GGCCGAGGTG ATGGTGGCTT CCCACAATGA GGACACCGTG 
AGCTTTACGT TGTGCAGGAT GAAGGAGTTA GGCCTGCATC CTGCTGATGG TCAGGTGTGC 
TTCGGACAGC TGCTGGGGAT GTGTGACCAA ATTAGCTTCC CACTAGGCCA GGCAGGCTTC 
CCTGTGTACA AGTATGTACC CTACGGGCCT GTGATGGAGG TGCTCCCGTA CCTGTCCCGC 
CGTGCCCTGG AGAACAGCAG CATCATGAAG GGTGCTCAGC GAGAGAGGCA GCTGCTATGG 
CAGGAGCTCT GCAGGCGGCT GCGCACAGGC AGCCTCTTCC ACCATCCGGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767125.1
CDS	99..1592
Translation	MFERLMKMTFYGHFVAGEDQESIRPLIRHNKAFGVGFILDYGVEEDLSPEEAERKEMESCTSEAERDGSGANKREKQYQVHPAFGDRRDGVISARTYFYASEAKCDNYMENLLQCIEASGGASDGGFSAIKLTALGRPQFLLQFSDVLTRWRQFFHQMAAEQGQAGRPAVDTKLEVAVLQESIAKMGISSRAEIERWFTPETLGVSGTVDLLDWNSLIDSRTQLSRHLVVPNVQTGQLEPLLSRFTEEEEQQMKRMLQRMDVLAKRAREACVRLMIDAEQSYFQPAISRLTLEMQRRFNVDKPFIFNTFQCYLKDAYDNVTIDMELSRREGWCFGAKLVRGAYMAQERARAVEIGYEDPINPTYEATNAMYHRCLNYVLEELKHSTKAEVMVASHNEDTVSFTLCRMKELGLHPADGQVCFGQLLGMCDQISFPLGQAGFPVYKYVPYGPVMEVLPYLSRRALENSSIMKGAQRERQLLWQELCRRLRTGSLFHHPA

Target ORF information:

RefSeq Version	XM_008768903.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proline dehydrogenase 1 (Prodh1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768903.2

ORF Insert Sequence:

ATGTTCGAGA GACTGATGAA GATGACCTTC TACGGCCATT TCGTGGCTGG CGAGGACCAG 
GAGTCTATCA GGCCTCTGAT TCGGCACAAC AAAGCCTTTG GTGTTGGCTT TATCCTGGAC 
TATGGTGTGG AGGAAGATCT GAGCCCCGAG GAGGCGGAGC GCAAAGAAAT GGAGTCATGC 
ACCTCTGAAG CTGAAAGAGA TGGCAGTGGA GCAAATAAGA GGGAGAAGCA GTATCAGGTC 
CACCCCGCCT TCGGAGACCG CAGAGATGGC GTCATCAGTG CCCGCACTTA CTTCTATGCC 
AGCGAAGCCA AATGTGACAA CTACATGGAG AACTTACTGC AGTGCATCGA GGCCTCAGGC 
GGAGCCAGTG ACGGTGGTTT TTCAGCCATT AAGCTCACGG CACTGGGGAG ACCACAGTTT 
CTGCTGCAGT TCTCAGACGT GCTGACCAGG TGGAGACAGT TTTTTCATCA AATGGCTGCA 
GAGCAGGGAC AGGCTGGGCG TCCTGCTGTG GACACGAAGC TGGAGGTGGC GGTGCTCCAG 
GAGAGCATCG CCAAGATGGG CATCTCGTCC AGGGCTGAGA TCGAGAGGTG GTTCACACCA 
GAGACGCTGG GAGTGTCTGG CACTGTGGAC CTACTGGACT GGAACAGCCT CATTGACAGC 
AGGACCCAGC TCTCCAGGCA CCTGGTGGTC CCCAATGTAC AGACTGGCCA GCTGGAGCCC 
CTGCTGTCAC GGTTCACTGA GGAGGAAGAG CAGCAGATGA AGAGGATGCT GCAGAGGATG 
GATGTGCTGG CCAAGAGAGC GAGAGAGGCA TGCGTGCGGC TGATGATTGA CGCCGAGCAG 
AGCTACTTCC AGCCGGCCAT CAGCCGCCTG ACCCTGGAGA TGCAGCGCAG GTTCAATGTG 
GATAAGCCGT TCATCTTCAA CACGTTCCAG TGCTACCTCA AGGATGCCTA TGACAATGTG 
ACCATAGATA TGGAACTGTC TCGCCGTGAG GGCTGGTGTT TTGGGGCCAA GCTGGTACGT 
GGTGCATACA TGGCCCAGGA GCGAGCCAGG GCTGTGGAGA TCGGCTACGA GGACCCCATC 
AACCCTACAT ATGAAGCCAC CAATGCCATG TACCACAGGT GCCTTAACTA TGTTCTGGAG 
GAGCTGAAGC ACAGCACAAA GGCCGAGGTG ATGGTGGCTT CCCACAATGA GGACACCGTG 
AGCTTTACGT TGTGCAGGAT GAAGGAGTTA GGCCTGCATC CTGCTGATGG TCAGGTGTGC 
TTCGGACAGC TGCTGGGGAT GTGTGACCAA ATTAGCTTCC CACTAGGCCA GGCAGGCTTC 
CCTGTGTACA AGTATGTACC CTACGGGCCT GTGATGGAGG TGCTCCCGTA CCTGTCCCGC 
CGTGCCCTGG AGAACAGCAG CATCATGAAG GGTGCTCAGC GAGAGAGGCA GCTGCTATGG 
CAGGAGCTCT GCAGGCGGCT GCGCACAGGC AGCCTCTTCC ACCATCCGGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prodh1 ( NM_001135778.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001129250.2 Rattus norvegicus proline dehydrogenase 1 (Prodh1), mRNA.

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CloneID	ORa63279
Clone ID Related Accession (Same CDS sequence)	NM_001135778.2
Accession Version	NM_001135778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	proline dehydrogenase 1, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07034748.1. On Jun 30, 2017 this sequence version replaced NM_001135778.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCTCTGA AGCGGGTCTT TTTGCTGCGC TCGGTGGCAC CACGCGTTGC TGCCCTCTCA 
ACCAAACCGC GAGCCCAGGA GCAGCCTCCC GCGAACCCTG AGGCTTTGCG GGGATGTGGA 
GCGGCTGAGG CTGTGCGGCC GCCTGTGCCA GCCGTGGACT TCACCAACAC ACAGGAGGCG 
TACCGCAGCC GTCGAAGCTG GGAACTGGCG CGCAACCTGC TAGTGCTGCG GCTGTGTGCG 
TCGCCGGTGC TGCTAGCGCA CCATGAGCAG TTGTTCCAAG TTGCCAGGAA GCTTCTGGGG 
CAGAGGATGT TCGAGAGACT GATGAAGATG ACCTTCTACG GCCATTTCGT GGCTGGCGAG 
GACCAGGAGT CTATCAGGCC TCTGATTCGG CACAACAAAG CCTTTGGTGT TGGCTTTATC 
CTGGACTATG GTGTGGAGGA AGATCTGAGC CCCGAGGAGG CGGAGCGCAA AGAAATGGAG 
TCATGCACCT CTGAAGCTGA AAGAGATGGC AGTGGAGCAA ATAAGAGGGA GAAGCAGTAT 
CAGGTCCACC CCGCCTTCGG AGACCGCAGA GATGGCGTCA TCAGTGCCCG CACTTACTTC 
TATGCCAGCG AAGCCAAATG TGACAACTAC ATGGAGAACT TACTGCAGTG CATCGAGGCC 
TCAGGCGGAG CCAGTGACGG TGGTTTTTCA GCCATTAAGC TCACGGCACT GGGGAGACCA 
CAGTTTCTGC TGCAGTTCTC AGACGTGCTG ACCAGGTGGA GACAGTTTTT TCATCAAATG 
GCTGCAGAGC AGGGACAGGC TGGGCGTCCT GCTGTGGACA CGAAGCTGGA GGTGGCGGTG 
CTCCAGGAGA GCATCGCCAA GATGGGCATC TCGTCCAGGG CTGAGATCGA GAGGTGGTTC 
ACACCAGAGA CGCTGGGAGT GTCTGGCACT GTGGACCTAC TGGACTGGAA CAGCCTCATT 
GACAGCAGGA CCCAGCTCTC CAGGCACCTG GTGGTCCCCA ATGTACAGAC TGGCCAGCTG 
GAGCCCCTGC TGTCACGGTT CACTGAGGAG GAAGAGCAGC AGATGAAGAG GATGCTGCAG 
AGGATGGATG TGCTGGCCAA GGTAGCGAGA GAGGCATGCG TGCGGCTGAT GATTGACGCC 
GAGCAGAGCT ACTTCCAGCC GGCCATCAGC CGCCTGACCC TGGAGATGCA GCGCAGGTTC 
AATGTGGATA AGCCGTTCAT CTTCAACACG TTCCAGTGCT ACCTCAAGGA TGCCTATGAC 
AATGTGACCA TAGATATGGA ACTGTCTCGC CGTGAGGGCT GGTGTTTTGG GGCCAAGCTG 
GTACGTGGTG CATACATGGC CCAGGAGCGA GCCAGGGCTG TGGAGATCGG CTACGAGGAC 
CCCATCAACC CTACATATGA AGCCACCAAT GCCATGTACC ACAGGTGCCT TAACTATGTT 
CTGGAGGAGC TGAAGCACAG CACAAAGGCC GAGGTGATGG TGGCTTCCCA CAATGAGGAC 
ACCGTGAGCT TTACGTTGTG CAGGATGAAG GAGTTAGGCC TGCATCCTGC TGATGGTCAG 
GTGTGCTTCG GACAGCTGCT GGGGATGTGT GACCAAATTA GCTTCCCACT AGGCCAGGCA 
GGCTTCCCTG TGTACAAGTA TGTACCCTAC GGGCCTGTGA TGGAGGTGCT CCCGTACCTG 
TCCCGCCGTG CCCTGGAGAA CAGCAGCATC ATGAAGGGTG CTCAGCGAGA GAGGCAGCTG 
CTATGGCAGG AGCTCTGCAG GCGGCTGCGC ACAGGCAGCC TCTTCCACCA TCCGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129250.2
CDS	25..1824
Translation	MALKRVFLLRSVAPRVAALSTKPRAQEQPPANPEALRGCGAAEAVRPPVPAVDFTNTQEAYRSRRSWELARNLLVLRLCASPVLLAHHEQLFQVARKLLGQRMFERLMKMTFYGHFVAGEDQESIRPLIRHNKAFGVGFILDYGVEEDLSPEEAERKEMESCTSEAERDGSGANKREKQYQVHPAFGDRRDGVISARTYFYASEAKCDNYMENLLQCIEASGGASDGGFSAIKLTALGRPQFLLQFSDVLTRWRQFFHQMAAEQGQAGRPAVDTKLEVAVLQESIAKMGISSRAEIERWFTPETLGVSGTVDLLDWNSLIDSRTQLSRHLVVPNVQTGQLEPLLSRFTEEEEQQMKRMLQRMDVLAKVAREACVRLMIDAEQSYFQPAISRLTLEMQRRFNVDKPFIFNTFQCYLKDAYDNVTIDMELSRREGWCFGAKLVRGAYMAQERARAVEIGYEDPINPTYEATNAMYHRCLNYVLEELKHSTKAEVMVASHNEDTVSFTLCRMKELGLHPADGQVCFGQLLGMCDQISFPLGQAGFPVYKYVPYGPVMEVLPYLSRRALENSSIMKGAQRERQLLWQELCRRLRTGSLFHHPA

Target ORF information:

RefSeq Version	NM_001135778.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proline dehydrogenase 1 (Prodh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135778.2

ORF Insert Sequence:

ATGGCTCTGA AGCGGGTCTT TTTGCTGCGC TCGGTGGCAC CACGCGTTGC TGCCCTCTCA 
ACCAAACCGC GAGCCCAGGA GCAGCCTCCC GCGAACCCTG AGGCTTTGCG GGGATGTGGA 
GCGGCTGAGG CTGTGCGGCC GCCTGTGCCA GCCGTGGACT TCACCAACAC ACAGGAGGCG 
TACCGCAGCC GTCGAAGCTG GGAACTGGCG CGCAACCTGC TAGTGCTGCG GCTGTGTGCG 
TCGCCGGTGC TGCTAGCGCA CCATGAGCAG TTGTTCCAAG TTGCCAGGAA GCTTCTGGGG 
CAGAGGATGT TCGAGAGACT GATGAAGATG ACCTTCTACG GCCATTTCGT GGCTGGCGAG 
GACCAGGAGT CTATCAGGCC TCTGATTCGG CACAACAAAG CCTTTGGTGT TGGCTTTATC 
CTGGACTATG GTGTGGAGGA AGATCTGAGC CCCGAGGAGG CGGAGCGCAA AGAAATGGAG 
TCATGCACCT CTGAAGCTGA AAGAGATGGC AGTGGAGCAA ATAAGAGGGA GAAGCAGTAT 
CAGGTCCACC CCGCCTTCGG AGACCGCAGA GATGGCGTCA TCAGTGCCCG CACTTACTTC 
TATGCCAGCG AAGCCAAATG TGACAACTAC ATGGAGAACT TACTGCAGTG CATCGAGGCC 
TCAGGCGGAG CCAGTGACGG TGGTTTTTCA GCCATTAAGC TCACGGCACT GGGGAGACCA 
CAGTTTCTGC TGCAGTTCTC AGACGTGCTG ACCAGGTGGA GACAGTTTTT TCATCAAATG 
GCTGCAGAGC AGGGACAGGC TGGGCGTCCT GCTGTGGACA CGAAGCTGGA GGTGGCGGTG 
CTCCAGGAGA GCATCGCCAA GATGGGCATC TCGTCCAGGG CTGAGATCGA GAGGTGGTTC 
ACACCAGAGA CGCTGGGAGT GTCTGGCACT GTGGACCTAC TGGACTGGAA CAGCCTCATT 
GACAGCAGGA CCCAGCTCTC CAGGCACCTG GTGGTCCCCA ATGTACAGAC TGGCCAGCTG 
GAGCCCCTGC TGTCACGGTT CACTGAGGAG GAAGAGCAGC AGATGAAGAG GATGCTGCAG 
AGGATGGATG TGCTGGCCAA GGTAGCGAGA GAGGCATGCG TGCGGCTGAT GATTGACGCC 
GAGCAGAGCT ACTTCCAGCC GGCCATCAGC CGCCTGACCC TGGAGATGCA GCGCAGGTTC 
AATGTGGATA AGCCGTTCAT CTTCAACACG TTCCAGTGCT ACCTCAAGGA TGCCTATGAC 
AATGTGACCA TAGATATGGA ACTGTCTCGC CGTGAGGGCT GGTGTTTTGG GGCCAAGCTG 
GTACGTGGTG CATACATGGC CCAGGAGCGA GCCAGGGCTG TGGAGATCGG CTACGAGGAC 
CCCATCAACC CTACATATGA AGCCACCAAT GCCATGTACC ACAGGTGCCT TAACTATGTT 
CTGGAGGAGC TGAAGCACAG CACAAAGGCC GAGGTGATGG TGGCTTCCCA CAATGAGGAC 
ACCGTGAGCT TTACGTTGTG CAGGATGAAG GAGTTAGGCC TGCATCCTGC TGATGGTCAG 
GTGTGCTTCG GACAGCTGCT GGGGATGTGT GACCAAATTA GCTTCCCACT AGGCCAGGCA 
GGCTTCCCTG TGTACAAGTA TGTACCCTAC GGGCCTGTGA TGGAGGTGCT CCCGTACCTG 
TCCCGCCGTG CCCTGGAGAA CAGCAGCATC ATGAAGGGTG CTCAGCGAGA GAGGCAGCTG 
CTATGGCAGG AGCTCTGCAG GCGGCTGCGC ACAGGCAGCC TCTTCCACCA TCCGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prodh1 ( XM_006248642.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248704.3 Rattus norvegicus proline dehydrogenase 1 (Prodh1), transcript variant X1, mRNA.

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CloneID	ORa57721
Clone ID Related Accession (Same CDS sequence)	XM_006248642.3
Accession Version	XM_006248642.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	proline dehydrogenase 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248642.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGA AGCGGGTCTT TTTGCTGCGC TCGGTGGCAC CACGCGTTGC TGCCCTCTCA 
ACCAAACCGC GAGCCCAGGA GCAGCCTCCC GCGAACCCTG AGGCTTTGCG GGGATGTGGA 
GCGGCTGAGG CTGTGCGGCC GCCTGTGCCA GCCGTGGACT TCACCAACAC ACAGGAGGCG 
TACCGCAGCC GTCGAAGCTG GGAACTGGCG CGCAACCTGC TAGTGCTGCG GCTGTGTGCG 
TCGCCGGTGC TGCTAGCGCA CCATGAGCAG TTGTTCCAAG TTGCCAGGAA GCTTCTGGGG 
CAGAGGATGT TCGAGAGACT GATGAAGATG ACCTTCTACG GCCATTTCGT GGCTGGCGAG 
GACCAGGAGT CTATCAGGCC TCTGATTCGG CACAACAAAG CCTTTGGTGT TGGCTTTATC 
CTGGACTATG GTGTGGAGGA AGATCTGAGC CCCGAGGAGG CGGAGCGCAA AGAAATGGAG 
TCATGCACCT CTGAAGCTGA AAGAGATGGC AGTGGAGCAA ATAAGAGGGA GAAGCAGTAT 
CAGGTCCACC CCGCCTTCGG AGACCGCAGA GATGGCGTCA TCAGTGCCCG CACTTACTTC 
TATGCCAGCG AAGCCAAATG TGACAACTAC ATGGAGAACT TACTGCAGTG CATCGAGGCC 
TCAGGCGGAG CCAGTGACGG TGGTTTTTCA GCCATTAAGC TCACGGCACT GGGGAGACCA 
CAGTTTCTGC TGCAGTTCTC AGACGTGCTG ACCAGGTGGA GACAGTTTTT TCATCAAATG 
GCTGCAGAGC AGGGACAGGC TGGGCGTCCT GCTGTGGACA CGAAGCTGGA GGTGGCGGTG 
CTCCAGGAGA GCATCGCCAA GATGGGCATC TCGTCCAGGG CTGAGATCGA GAGGTGGTTC 
ACACCAGAGA CGCTGGGAGT GTCTGGCACT GTGGACCTAC TGGACTGGAA CAGCCTCATT 
GACAGCAGGA CCCAGCTCTC CAGGCACCTG GTGGTCCCCA ATGTACAGAC TGGCCAGCTG 
GAGCCCCTGC TGTCACGGTT CACTGAGGAG GAAGAGCAGC AGATGAAGAG GATGCTGCAG 
AGGATGGATG TGCTGGCCAA GAGAGCGAGA GAGGCATGCG TGCGGCTGAT GATTGACGCC 
GAGCAGAGCT ACTTCCAGCC GGCCATCAGC CGCCTGACCC TGGAGATGCA GCGCAGGTTC 
AATGTGGATA AGCCGTTCAT CTTCAACACG TTCCAGTGCT ACCTCAAGGA TGCCTATGAC 
AATGTGACCA TAGATATGGA ACTGTCTCGC CGTGAGGGCT GGTGTTTTGG GGCCAAGCTG 
GTACGTGGTG CATACATGGC CCAGGAGCGA GCCAGGGCTG TGGAGATCGG CTACGAGGAC 
CCCATCAACC CTACATATGA AGCCACCAAT GCCATGTACC ACAGGTGCCT TAACTATGTT 
CTGGAGGAGC TGAAGCACAG CACAAAGGCC GAGGTGATGG TGGCTTCCCA CAATGAGGAC 
ACCGTGAGCT TTACGTTGTG CAGGATGAAG GAGTTAGGCC TGCATCCTGC TGATGGTCAG 
GTGTGCTTCG GACAGCTGCT GGGGATGTGT GACCAAATTA GCTTCCCACT AGGCCAGGCA 
GGCTTCCCTG TGTACAAGTA TGTACCCTAC GGGCCTGTGA TGGAGGTGCT CCCGTACCTG 
TCCCGCCGTG CCCTGGAGAA CAGCAGCATC ATGAAGGGTG CTCAGCGAGA GAGGCAGCTG 
CTATGGCAGG AGCTCTGCAG GCGGCTGCGC ACAGGCAGCC TCTTCCACCA TCCGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248704.3
CDS	31..1830
Translation	MALKRVFLLRSVAPRVAALSTKPRAQEQPPANPEALRGCGAAEAVRPPVPAVDFTNTQEAYRSRRSWELARNLLVLRLCASPVLLAHHEQLFQVARKLLGQRMFERLMKMTFYGHFVAGEDQESIRPLIRHNKAFGVGFILDYGVEEDLSPEEAERKEMESCTSEAERDGSGANKREKQYQVHPAFGDRRDGVISARTYFYASEAKCDNYMENLLQCIEASGGASDGGFSAIKLTALGRPQFLLQFSDVLTRWRQFFHQMAAEQGQAGRPAVDTKLEVAVLQESIAKMGISSRAEIERWFTPETLGVSGTVDLLDWNSLIDSRTQLSRHLVVPNVQTGQLEPLLSRFTEEEEQQMKRMLQRMDVLAKRAREACVRLMIDAEQSYFQPAISRLTLEMQRRFNVDKPFIFNTFQCYLKDAYDNVTIDMELSRREGWCFGAKLVRGAYMAQERARAVEIGYEDPINPTYEATNAMYHRCLNYVLEELKHSTKAEVMVASHNEDTVSFTLCRMKELGLHPADGQVCFGQLLGMCDQISFPLGQAGFPVYKYVPYGPVMEVLPYLSRRALENSSIMKGAQRERQLLWQELCRRLRTGSLFHHPA

Target ORF information:

RefSeq Version	XM_006248642.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proline dehydrogenase 1 (Prodh1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248642.3

ORF Insert Sequence:

ATGGCTCTGA AGCGGGTCTT TTTGCTGCGC TCGGTGGCAC CACGCGTTGC TGCCCTCTCA 
ACCAAACCGC GAGCCCAGGA GCAGCCTCCC GCGAACCCTG AGGCTTTGCG GGGATGTGGA 
GCGGCTGAGG CTGTGCGGCC GCCTGTGCCA GCCGTGGACT TCACCAACAC ACAGGAGGCG 
TACCGCAGCC GTCGAAGCTG GGAACTGGCG CGCAACCTGC TAGTGCTGCG GCTGTGTGCG 
TCGCCGGTGC TGCTAGCGCA CCATGAGCAG TTGTTCCAAG TTGCCAGGAA GCTTCTGGGG 
CAGAGGATGT TCGAGAGACT GATGAAGATG ACCTTCTACG GCCATTTCGT GGCTGGCGAG 
GACCAGGAGT CTATCAGGCC TCTGATTCGG CACAACAAAG CCTTTGGTGT TGGCTTTATC 
CTGGACTATG GTGTGGAGGA AGATCTGAGC CCCGAGGAGG CGGAGCGCAA AGAAATGGAG 
TCATGCACCT CTGAAGCTGA AAGAGATGGC AGTGGAGCAA ATAAGAGGGA GAAGCAGTAT 
CAGGTCCACC CCGCCTTCGG AGACCGCAGA GATGGCGTCA TCAGTGCCCG CACTTACTTC 
TATGCCAGCG AAGCCAAATG TGACAACTAC ATGGAGAACT TACTGCAGTG CATCGAGGCC 
TCAGGCGGAG CCAGTGACGG TGGTTTTTCA GCCATTAAGC TCACGGCACT GGGGAGACCA 
CAGTTTCTGC TGCAGTTCTC AGACGTGCTG ACCAGGTGGA GACAGTTTTT TCATCAAATG 
GCTGCAGAGC AGGGACAGGC TGGGCGTCCT GCTGTGGACA CGAAGCTGGA GGTGGCGGTG 
CTCCAGGAGA GCATCGCCAA GATGGGCATC TCGTCCAGGG CTGAGATCGA GAGGTGGTTC 
ACACCAGAGA CGCTGGGAGT GTCTGGCACT GTGGACCTAC TGGACTGGAA CAGCCTCATT 
GACAGCAGGA CCCAGCTCTC CAGGCACCTG GTGGTCCCCA ATGTACAGAC TGGCCAGCTG 
GAGCCCCTGC TGTCACGGTT CACTGAGGAG GAAGAGCAGC AGATGAAGAG GATGCTGCAG 
AGGATGGATG TGCTGGCCAA GAGAGCGAGA GAGGCATGCG TGCGGCTGAT GATTGACGCC 
GAGCAGAGCT ACTTCCAGCC GGCCATCAGC CGCCTGACCC TGGAGATGCA GCGCAGGTTC 
AATGTGGATA AGCCGTTCAT CTTCAACACG TTCCAGTGCT ACCTCAAGGA TGCCTATGAC 
AATGTGACCA TAGATATGGA ACTGTCTCGC CGTGAGGGCT GGTGTTTTGG GGCCAAGCTG 
GTACGTGGTG CATACATGGC CCAGGAGCGA GCCAGGGCTG TGGAGATCGG CTACGAGGAC 
CCCATCAACC CTACATATGA AGCCACCAAT GCCATGTACC ACAGGTGCCT TAACTATGTT 
CTGGAGGAGC TGAAGCACAG CACAAAGGCC GAGGTGATGG TGGCTTCCCA CAATGAGGAC 
ACCGTGAGCT TTACGTTGTG CAGGATGAAG GAGTTAGGCC TGCATCCTGC TGATGGTCAG 
GTGTGCTTCG GACAGCTGCT GGGGATGTGT GACCAAATTA GCTTCCCACT AGGCCAGGCA 
GGCTTCCCTG TGTACAAGTA TGTACCCTAC GGGCCTGTGA TGGAGGTGCT CCCGTACCTG 
TCCCGCCGTG CCCTGGAGAA CAGCAGCATC ATGAAGGGTG CTCAGCGAGA GAGGCAGCTG 
CTATGGCAGG AGCTCTGCAG GCGGCTGCGC ACAGGCAGCC TCTTCCACCA TCCGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DJ-1 cooperates with PYCR1 in cell protection against oxidative stress.
Biochemical and biophysical research communications436(2)289-94(2013 Jun)
Yasuda T,Kaji Y,Agatsuma T,Niki T,Arisawa M,Shuto S,Ariga H,Iguchi-Ariga SM

Mitochondrial localization and function of a subset of 22q11 deletion syndrome candidate genes.
Molecular and cellular neurosciences39(3)439-51(2008 Nov)
Maynard TM,Meechan DW,Dudevoir ML,Gopalakrishna D,Peters AZ,Heindel CC,Sugimoto TJ,Wu Y,Lieberman JA,Lamantia AS

Functional consequences of PRODH missense mutations.
American journal of human genetics76(3)409-20(2005 Mar)
Bender HU,Almashanu S,Steel G,Hu CA,Lin WW,Willis A,Pulver A,Valle D

Proteomic analysis of the mouse liver mitochondrial inner membrane.
The Journal of biological chemistry278(42)41566-71(2003 Oct)
Da Cruz S,Xenarios I,Langridge J,Vilbois F,Parone PA,Martinou JC

The severe form of type I hyperprolinaemia results from homozygous inactivation of the PRODH gene.
Journal of medical genetics40(1)e7(2003 Jan)
Jacquet H,Berthelot J,Bonnemains C,Simard G,Saugier-Veber P,Raux G,Campion D,Bonneau D,Frebourg T

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Prodh1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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