POLR2C cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR2C
Entrez Gene ID	5432
Full Name	RNA polymerase II subunit C
Synonyms	RPB3,RPB31,hRPB33,hsRPB3
General protein information	Preferred Names RNA polymerase II subunit C Names DNA-directed RNA polymerase II subunit RPB3 DNA-directed RNA polymerase II 33 kDa polypeptide DNA-directed RNA polymerase II subunit C RNA polymerase II subunit 3 RNA polymerase II subunit B3 RPB33 polymerase (RNA) II (DNA directed) polypeptide C, 33kDa polymerase (RNA) II subunit C
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q21
Summary	This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. [provided by RefSeq, Jul 2008].
Disorder MIM:	180663

mRNA and Protein(s)

mRNA	Protein	Name
NM_032940.2	NP_116558.1	DNA-directed RNA polymerase II subunit RPB3
NM_032940.3	NP_116558.1	DNA-directed RNA polymerase II subunit RPB3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	POLR2C	XP_001148305.1
Mus musculus (house mouse)	Polr2c	NP_033116.2
Drosophila melanogaster (fruit fly)	RpII33	NP_477419.1
Caenorhabditis elegans (roundworm)	rpb-3	NP_492361.2
Homo sapiens (human)	POLR2C	NP_116558.1
	POLR2C	XP_001098417.1
Bos taurus (cattle)	POLR2C	NP_001029384.1
Neurospora crassa	NCU01515	XP_956964.1
Arabidopsis thaliana (thale cress)	NRPB3	NP_179145.1
Gallus gallus (chicken)	POLR2C	XP_414001.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010321	XP_318245.2
Arabidopsis thaliana (thale cress)	NRPE3B	NP_179142.1
Oryza sativa (rice)	Os09g0110400	NP_001062572.1
Rattus norvegicus (Norway rat)	Polr2c	NP_001012491.1
Saccharomyces cerevisiae (baker's yeast)	RPB3	NP_012243.3
Eremothecium gossypii	AGOS_ADL120C	NP_983976.1
Schizosaccharomyces pombe (fission yeast)	rpb3	NP_588324.1
Magnaporthe oryzae (rice blast fungus)	MGG_03215	XP_003716764.1
Canis lupus familiaris (dog)	POLR2C	XP_535283.2
Danio rerio (zebrafish)	polr2c	NP_001161428.1
Kluyveromyces lactis	KLLA0D16170g	XP_453773.1
Xenopus tropicalis (tropical clawed frog)	polr2c	NP_989124.1

Gene Ontology
Bibliography

Related articles in PubMed

Residue-by-Residue View of In?Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II.
Burke KA, Janke AM, Rhine CL, Fawzi NL
Molecular cell60(2)231-41(2015 Oct)

Rpb3 promotes hepatocellular carcinoma through its N-terminus.
Fang ZP, Jiang BG, Zhang FB, Wang AD, Ji YM, Xu YF, Li JC, Zhou WP, Zhou WJ, Han HX
Oncotarget5(19)9256-68(2014 Oct)

The eEF1? subunit contacts RNA polymerase II and binds vimentin promoter region.
Corbi N, Batassa EM, Pisani C, Onori A, Di Certo MG, Strimpakos G, Fanciulli M, Mattei E, Passananti C
PloS one5(12)e14481(2010 Dec)

Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk.
Michiels S, Laplanche A, Boulet T, Dessen P, Guillonneau B, M?jean A, Desgrandchamps F, Lathrop M, Sarasin A, Benhamou S
Carcinogenesis30(5)763-8(2009 May)

Ribonucleic acid polymerase II binding subunit 3 (Rpb3), a potential nuclear target of insulin-like growth factor binding protein-3.
Oufattole M, Lin SW, Liu B, Mascarenhas D, Cohen P, Rodgers BD
Endocrinology147(5)2138-46(2006 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FUS low-complexicity phase separates to recruit RNA polymerase II C-terminal domain in vitro.
Title: Residue-by-Residue View of In?Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II.

These findings suggest that N-terminus of Rpb3 selectively inhibits Rpb3-high-expression hepatocellular carcinoma cells proliferation. N-terminus of Rpb3 may be useful in treating patients diagnosed with Rpb3-high-expression hepatocellular carcinoma.
Title: Rpb3 promotes hepatocellular carcinoma through its N-terminus.

eEF1gamma, in addition to its role in translation elongation complex, is involved in regulating Vimentin gene by contacting both pol II and the Vimentin promoter region and shuttling/nursing the Vimentin mRNA
Title: The eEF1? subunit contacts RNA polymerase II and binds vimentin promoter region.

Observational study of gene-disease association, gene-gene interaction, and gene-environment interaction. (HuGE Navigator)
Title: Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk.

Rpb3 is a potential nuclear target of insulin-like growth factor binding protein-3
Title: Ribonucleic acid polymerase II binding subunit 3 (Rpb3), a potential nuclear target of insulin-like growth factor binding protein-3.

The following POLR2C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR2C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30415	NM_032940.3 Latest version!	Homo sapiens RNA polymerase II subunit C (POLR2C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu30415	NM_032940.2	Homo sapiens RNA polymerase II subunit C (POLR2C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR2C ( NM_032940.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_116558.1 Homo sapiens RNA polymerase II subunit C (POLR2C), mRNA.

Price & Availability↑

CloneID	OHu30415
Clone ID Related Accession (Same CDS sequence)	NM_032940.2 , NM_032940.3
Accession Version	NM_032940.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI458170.1 and BC003159.1. On Mar 9, 2006 this sequence version replaced NM_032940.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003159.1, SRR1163657.6362.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCGTACG CCAACCAGCC TACCGTGCGG ATCACGGAGC TCACTGACGA GAATGTCAAG 
TTCATCATCG AGAACACCGA CCTGGCGGTG GCCAATTCGA TTCGGAGGGT CTTCATCGCT 
GAGGTTCCCA TAATAGCCAT TGACTGGGTT CAGATTGATG CCAATTCCTC AGTTCTTCAT 
GATGAATTCA TTGCTCACAG GCTTGGATTA ATTCCCCTCA TTAGTGATGA CATTGTGGAC 
AAGCTGCAGT ACTCTCGGGA CTGCACATGT GAGGAGTTCT GCCCCGAGTG CTCGGTGGAG 
TTCACCCTCG ATGTGCGGTG CAATGAAGAC CAGACGCGAC ATGTCACGTC TCGAGACCTC 
ATCTCCAACA GCCCCCGGGT CATTCCGGTG ACATCCCGGA ACCGAGATAA TGACCCCAAT 
GACTACGTGG AGCAGGATGA CATCCTCATC GTCAAGTTGA GAAAGGGCCA GGAGCTGAGA 
CTTCGAGCCT ATGCCAAAAA GGGCTTTGGC AAGGAGCATG CCAAGTGGAA CCCTACTGCA 
GGGGTGGCTT TTGAATACGA TCCAGACAAT GCCCTGAGGC ACACAGTGTA CCCCAAGCCC 
GAGGAATGGC CAAAGAGTGA GTACTCGGAG CTGGATGAGG ATGAGTCGCA GGCTCCCTAT 
GACCCCAACG GCAAGCCAGA AAGGTTTTAC TACAATGTGG AGTCCTGTGG CTCTCTGCGT 
CCTGAAACCA TTGTCCTGTC AGCCCTCTCA GGATTGAAGA AGAAACTGAG TGATTTACAA 
ACTCAATTAA GCCACGAGAT CCAGAGTGAT GTGCTAACCA TAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116558.1
CDS	87..914
Translation	MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPNDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTAGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERFYYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN

Target ORF information:

RefSeq Version	NM_032940.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit C (POLR2C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032940.2

ORF Insert Sequence:

ATGCCGTACG CCAACCAGCC TACCGTGCGG ATCACGGAGC TCACTGACGA GAATGTCAAG 
TTCATCATCG AGAACACCGA CCTGGCGGTG GCCAATTCGA TTCGGAGGGT CTTCATCGCT 
GAGGTTCCCA TAATAGCCAT TGACTGGGTT CAGATTGATG CCAATTCCTC AGTTCTTCAT 
GATGAATTCA TTGCTCACAG GCTTGGATTA ATTCCCCTCA TTAGTGATGA CATTGTGGAC 
AAGCTGCAGT ACTCTCGGGA CTGCACATGT GAGGAGTTCT GCCCCGAGTG CTCGGTGGAG 
TTCACCCTCG ATGTGCGGTG CAATGAAGAC CAGACGCGAC ATGTCACGTC TCGAGACCTC 
ATCTCCAACA GCCCCCGGGT CATTCCGGTG ACATCCCGGA ACCGAGATAA TGACCCCAAT 
GACTACGTGG AGCAGGATGA CATCCTCATC GTCAAGTTGA GAAAGGGCCA GGAGCTGAGA 
CTTCGAGCCT ATGCCAAAAA GGGCTTTGGC AAGGAGCATG CCAAGTGGAA CCCTACTGCA 
GGGGTGGCTT TTGAATACGA TCCAGACAAT GCCCTGAGGC ACACAGTGTA CCCCAAGCCC 
GAGGAATGGC CAAAGAGTGA GTACTCGGAG CTGGATGAGG ATGAGTCGCA GGCTCCCTAT 
GACCCCAACG GCAAGCCAGA AAGGTTTTAC TACAATGTGG AGTCCTGTGG CTCTCTGCGT 
CCTGAAACCA TTGTCCTGTC AGCCCTCTCA GGATTGAAGA AGAAACTGAG TGATTTACAA 
ACTCAATTAA GCCACGAGAT CCAGAGTGAT GTGCTAACCA TAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR2C ( NM_032940.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_116558.1 Homo sapiens RNA polymerase II subunit C (POLR2C), mRNA.

Price & Availability↑

CloneID	OHu30415
Clone ID Related Accession (Same CDS sequence)	NM_032940.2 , NM_032940.3
Accession Version	NM_032940.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI458170.1 and BC003159.1. On Nov 23, 2018 this sequence version replaced NM_032940.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003159.1, SRR3476690.801283.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000219252.10/ ENSP00000219252.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGTACG CCAACCAGCC TACCGTGCGG ATCACGGAGC TCACTGACGA GAATGTCAAG 
TTCATCATCG AGAACACCGA CCTGGCGGTG GCCAATTCGA TTCGGAGGGT CTTCATCGCT 
GAGGTTCCCA TAATAGCCAT TGACTGGGTT CAGATTGATG CCAATTCCTC AGTTCTTCAT 
GATGAATTCA TTGCTCACAG GCTTGGATTA ATTCCCCTCA TTAGTGATGA CATTGTGGAC 
AAGCTGCAGT ACTCTCGGGA CTGCACATGT GAGGAGTTCT GCCCCGAGTG CTCGGTGGAG 
TTCACCCTCG ATGTGCGGTG CAATGAAGAC CAGACGCGAC ATGTCACGTC TCGAGACCTC 
ATCTCCAACA GCCCCCGGGT CATTCCGGTG ACATCCCGGA ACCGAGATAA TGACCCCAAT 
GACTACGTGG AGCAGGATGA CATCCTCATC GTCAAGTTGA GAAAGGGCCA GGAGCTGAGA 
CTTCGAGCCT ATGCCAAAAA GGGCTTTGGC AAGGAGCATG CCAAGTGGAA CCCTACTGCA 
GGGGTGGCTT TTGAATACGA TCCAGACAAT GCCCTGAGGC ACACAGTGTA CCCCAAGCCC 
GAGGAATGGC CAAAGAGTGA GTACTCGGAG CTGGATGAGG ATGAGTCGCA GGCTCCCTAT 
GACCCCAACG GCAAGCCAGA AAGGTTTTAC TACAATGTGG AGTCCTGTGG CTCTCTGCGT 
CCTGAAACCA TTGTCCTGTC AGCCCTCTCA GGATTGAAGA AGAAACTGAG TGATTTACAA 
ACTCAATTAA GCCACGAGAT CCAGAGTGAT GTGCTAACCA TAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116558.1
CDS	47..874
Translation	MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPNDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTAGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERFYYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN

Target ORF information:

RefSeq Version	NM_032940.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit C (POLR2C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032940.3

ORF Insert Sequence:

ATGCCGTACG CCAACCAGCC TACCGTGCGG ATCACGGAGC TCACTGACGA GAATGTCAAG 
TTCATCATCG AGAACACCGA CCTGGCGGTG GCCAATTCGA TTCGGAGGGT CTTCATCGCT 
GAGGTTCCCA TAATAGCCAT TGACTGGGTT CAGATTGATG CCAATTCCTC AGTTCTTCAT 
GATGAATTCA TTGCTCACAG GCTTGGATTA ATTCCCCTCA TTAGTGATGA CATTGTGGAC 
AAGCTGCAGT ACTCTCGGGA CTGCACATGT GAGGAGTTCT GCCCCGAGTG CTCGGTGGAG 
TTCACCCTCG ATGTGCGGTG CAATGAAGAC CAGACGCGAC ATGTCACGTC TCGAGACCTC 
ATCTCCAACA GCCCCCGGGT CATTCCGGTG ACATCCCGGA ACCGAGATAA TGACCCCAAT 
GACTACGTGG AGCAGGATGA CATCCTCATC GTCAAGTTGA GAAAGGGCCA GGAGCTGAGA 
CTTCGAGCCT ATGCCAAAAA GGGCTTTGGC AAGGAGCATG CCAAGTGGAA CCCTACTGCA 
GGGGTGGCTT TTGAATACGA TCCAGACAAT GCCCTGAGGC ACACAGTGTA CCCCAAGCCC 
GAGGAATGGC CAAAGAGTGA GTACTCGGAG CTGGATGAGG ATGAGTCGCA GGCTCCCTAT 
GACCCCAACG GCAAGCCAGA AAGGTTTTAC TACAATGTGG AGTCCTGTGG CTCTCTGCGT 
CCTGAAACCA TTGTCCTGTC AGCCCTCTCA GGATTGAAGA AGAAACTGAG TGATTTACAA 
ACTCAATTAA GCCACGAGAT CCAGAGTGAT GTGCTAACCA TAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Residue-by-Residue View of In?Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II.
Molecular cell60(2)231-41(2015 Oct)
Burke KA,Janke AM,Rhine CL,Fawzi NL

Rpb3 promotes hepatocellular carcinoma through its N-terminus.
Oncotarget5(19)9256-68(2014 Oct)
Fang ZP,Jiang BG,Zhang FB,Wang AD,Ji YM,Xu YF,Li JC,Zhou WP,Zhou WJ,Han HX

The eEF1? subunit contacts RNA polymerase II and binds vimentin promoter region.
PloS one5(12)e14481(2010 Dec)
Corbi N,Batassa EM,Pisani C,Onori A,Di Certo MG,Strimpakos G,Fanciulli M,Mattei E,Passananti C

Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk.
Carcinogenesis30(5)763-8(2009 May)
Michiels S,Laplanche A,Boulet T,Dessen P,Guillonneau B,M?jean A,Desgrandchamps F,Lathrop M,Sarasin A,Benhamou S

Ribonucleic acid polymerase II binding subunit 3 (Rpb3), a potential nuclear target of insulin-like growth factor binding protein-3.
Endocrinology147(5)2138-46(2006 May)
Oufattole M,Lin SW,Liu B,Mascarenhas D,Cohen P,Rodgers BD

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POLR2C cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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