PLA2G1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLA2G1B
Entrez Gene ID	5319
Full Name	phospholipase A2 group IB
Synonyms	PLA2,PLA2A,PPLA2
General protein information	Preferred Names phospholipase A2 group IB Names phospholipase A2 phosphatidylcholine 2-acylhydrolase 1B phospholipase A2, group IB (pancreas)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.31
Summary	This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013].
Disorder MIM:	172410

mRNA and Protein(s)

mRNA	Protein	Name
NM_000928.3	NP_000919.1	phospholipase A2 precursor
NM_000928.2	NP_000919.1	phospholipase A2 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pla2g1b	NP_113773.1
Xenopus tropicalis (tropical clawed frog)	pla2g1b	XP_004910660.1
	PLA2G1B	XP_001088684.1
Canis lupus familiaris (dog)	PLA2G1B	NP_001003320.1
Mus musculus (house mouse)	Pla2g1b	NP_035237.1
Gallus gallus (chicken)	PLA2G1B	XP_001233627.2
Homo sapiens (human)	PLA2G1B	NP_000919.1
Pan troglodytes (chimpanzee)	PLA2G1B	XP_001160305.1
Bos taurus (cattle)	PLA2G1B	NP_777071.2
Danio rerio (zebrafish)	pla2g1b	XP_700448.2

Gene Ontology
Bibliography

Related articles in PubMed

sPLA2 IB induces human podocyte apoptosis via the M-type phospholipase A2 receptor.
Pan Y, Wan J, Liu Y, Yang Q, Liang W, Singhal PC, Saleem MA, Ding G
Scientific reports46660(2014 Oct)

TNF-? induces cytosolic phospholipase A2 expression via Jak2/PDGFR-dependent Elk-1/p300 activation in human lung epithelial cells.
Yang CM, Lee IT, Chi PL, Cheng SE, Hsiao LD, Hsu CK
American journal of physiology. Lung cellular and molecular physiology306(6)L543-51(2014 Mar)

Diverse regulation of retinal pigment epithelium phagocytosis of photoreceptor outer segments by calcium-independent phospholipase A?, group VIA and secretory phospholipase A?, group IB.
Zhan C, Wang J, Kolko M
Current eye research37(10)930-40(2012 Oct)

Tissue-specific transplantation antigen P35B (TSTA3) immune response-mediated metabolism coupling cell cycle to postreplication repair network in no-tumor hepatitis/cirrhotic tissues (HBV or HCV infection) by biocomputation.
Wang L, Huang J, Jiang M, Lin H
Immunologic research52(3)258-68(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The sPLA2 IB-PLA2R interaction stimulated podocyte apoptosis through activating ERK1/2 and cPLA2alpha and through increasing the podocyte AA content
Title: sPLA2 IB induces human podocyte apoptosis via the M-type phospholipase A2 receptor.

TNF-alpha-induced cPLA2 expression and PGE2 release were mediated through a Jak2/PDGFR/PI3K/Akt/p42/p44 MAPK/Elk-1 pathway in human lung epithelial cells.
Title: TNF-? induces cytosolic phospholipase A2 expression via Jak2/PDGFR-dependent Elk-1/p300 activation in human lung epithelial cells.

Exogenously added sPLA(2)-IB decreases phagocytosis of photoreceptor outer segments regardless of enzymatic activity.
Title: Diverse regulation of retinal pigment epithelium phagocytosis of photoreceptor outer segments by calcium-independent phospholipase A?, group VIA and secretory phospholipase A?, group IB.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following PLA2G1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLA2G1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25603	NM_000928.3 Latest version!	Homo sapiens phospholipase A2 group IB (PLA2G1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu25603	NM_000928.2	Homo sapiens phospholipase A2 group IB (PLA2G1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLA2G1B ( NM_000928.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000919.1 Homo sapiens phospholipase A2 group IB (PLA2G1B), mRNA.

Price & Availability↑

CloneID	OHu25603
Clone ID Related Accession (Same CDS sequence)	NM_000928.3 , NM_000928.2
Accession Version	NM_000928.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	phospholipase A2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M21054.1 and AA844927.1. On Nov 22, 2018 this sequence version replaced NM_000928.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP322008.1, BP322424.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2147920 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000308366.9/ ENSP00000312286.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAACTCC TTGTGCTAGC TGTGCTGCTC ACAGTGGCCG CCGCCGACAG CGGCATCAGC 
CCTCGGGCCG TGTGGCAGTT CCGCAAAATG ATCAAGTGCG TGATCCCGGG GAGTGACCCC 
TTCTTGGAAT ACAACAACTA CGGCTGCTAC TGTGGCTTGG GGGGCTCAGG CACCCCCGTG 
GATGAACTGG ACAAGTGCTG CCAGACACAT GACAACTGCT ATGACCAGGC CAAGAAGCTG 
GACAGCTGTA AATTTCTGCT GGACAACCCG TACACCCACA CCTATTCATA CTCGTGCTCT 
GGCTCGGCAA TCACCTGTAG CAGCAAAAAC AAAGAGTGTG AGGCCTTCAT TTGCAACTGC 
GACCGCAACG CTGCCATCTG CTTTTCAAAA GCTCCATATA ACAAGGCACA CAAGAACCTG 
GACACCAAGA AGTATTGTCA GAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000919.1
CDS	27..473
Translation	MKLLVLAVLLTVAAADSGISPRAVWQFRKMIKCVIPGSDPFLEYNNYGCYCGLGGSGTPVDELDKCCQTHDNCYDQAKKLDSCKFLLDNPYTHTYSYSCSGSAITCSSKNKECEAFICNCDRNAAICFSKAPYNKAHKNLDTKKYCQS

Target ORF information:

RefSeq Version	NM_000928.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IB (PLA2G1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000928.3

ORF Insert Sequence:

ATGAAACTCC TTGTGCTAGC TGTGCTGCTC ACAGTGGCCG CCGCCGACAG CGGCATCAGC 
CCTCGGGCCG TGTGGCAGTT CCGCAAAATG ATCAAGTGCG TGATCCCGGG GAGTGACCCC 
TTCTTGGAAT ACAACAACTA CGGCTGCTAC TGTGGCTTGG GGGGCTCAGG CACCCCCGTG 
GATGAACTGG ACAAGTGCTG CCAGACACAT GACAACTGCT ATGACCAGGC CAAGAAGCTG 
GACAGCTGTA AATTTCTGCT GGACAACCCG TACACCCACA CCTATTCATA CTCGTGCTCT 
GGCTCGGCAA TCACCTGTAG CAGCAAAAAC AAAGAGTGTG AGGCCTTCAT TTGCAACTGC 
GACCGCAACG CTGCCATCTG CTTTTCAAAA GCTCCATATA ACAAGGCACA CAAGAACCTG 
GACACCAAGA AGTATTGTCA GAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G1B ( NM_000928.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000919.1 Homo sapiens phospholipase A2 group IB (PLA2G1B), mRNA.

Price & Availability↑

CloneID	OHu25603
Clone ID Related Accession (Same CDS sequence)	NM_000928.3 , NM_000928.2
Accession Version	NM_000928.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	phospholipase A2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M21054.1, AA844927.1, CA867923.1, BX113838.1 and BE969737.1. On Oct 29, 2003 this sequence version replaced NM_000928.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC106725.1, BP323356.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2147920 [ECO:0000348] ##Evidence-Data-END##

ATGAAACTCC TTGTGCTAGC TGTGCTGCTC ACAGTGGCCG CCGCCGACAG CGGCATCAGC 
CCTCGGGCCG TGTGGCAGTT CCGCAAAATG ATCAAGTGCG TGATCCCGGG GAGTGACCCC 
TTCTTGGAAT ACAACAACTA CGGCTGCTAC TGTGGCTTGG GGGGCTCAGG CACCCCCGTG 
GATGAACTGG ACAAGTGCTG CCAGACACAT GACAACTGCT ATGACCAGGC CAAGAAGCTG 
GACAGCTGTA AATTTCTGCT GGACAACCCG TACACCCACA CCTATTCATA CTCGTGCTCT 
GGCTCGGCAA TCACCTGTAG CAGCAAAAAC AAAGAGTGTG AGGCCTTCAT TTGCAACTGC 
GACCGCAACG CTGCCATCTG CTTTTCAAAA GCTCCATATA ACAAGGCACA CAAGAACCTG 
GACACCAAGA AGTATTGTCA GAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000919.1
CDS	37..483
Translation	MKLLVLAVLLTVAAADSGISPRAVWQFRKMIKCVIPGSDPFLEYNNYGCYCGLGGSGTPVDELDKCCQTHDNCYDQAKKLDSCKFLLDNPYTHTYSYSCSGSAITCSSKNKECEAFICNCDRNAAICFSKAPYNKAHKNLDTKKYCQS

Target ORF information:

RefSeq Version	NM_000928.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IB (PLA2G1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000928.2

ORF Insert Sequence:

ATGAAACTCC TTGTGCTAGC TGTGCTGCTC ACAGTGGCCG CCGCCGACAG CGGCATCAGC 
CCTCGGGCCG TGTGGCAGTT CCGCAAAATG ATCAAGTGCG TGATCCCGGG GAGTGACCCC 
TTCTTGGAAT ACAACAACTA CGGCTGCTAC TGTGGCTTGG GGGGCTCAGG CACCCCCGTG 
GATGAACTGG ACAAGTGCTG CCAGACACAT GACAACTGCT ATGACCAGGC CAAGAAGCTG 
GACAGCTGTA AATTTCTGCT GGACAACCCG TACACCCACA CCTATTCATA CTCGTGCTCT 
GGCTCGGCAA TCACCTGTAG CAGCAAAAAC AAAGAGTGTG AGGCCTTCAT TTGCAACTGC 
GACCGCAACG CTGCCATCTG CTTTTCAAAA GCTCCATATA ACAAGGCACA CAAGAACCTG 
GACACCAAGA AGTATTGTCA GAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

sPLA2 IB induces human podocyte apoptosis via the M-type phospholipase A2 receptor.
Scientific reports46660(2014 Oct)
Pan Y,Wan J,Liu Y,Yang Q,Liang W,Singhal PC,Saleem MA,Ding G

TNF-? induces cytosolic phospholipase A2 expression via Jak2/PDGFR-dependent Elk-1/p300 activation in human lung epithelial cells.
American journal of physiology. Lung cellular and molecular physiology306(6)L543-51(2014 Mar)
Yang CM,Lee IT,Chi PL,Cheng SE,Hsiao LD,Hsu CK

Diverse regulation of retinal pigment epithelium phagocytosis of photoreceptor outer segments by calcium-independent phospholipase A?, group VIA and secretory phospholipase A?, group IB.
Current eye research37(10)930-40(2012 Oct)
Zhan C,Wang J,Kolko M

Tissue-specific transplantation antigen P35B (TSTA3) immune response-mediated metabolism coupling cell cycle to postreplication repair network in no-tumor hepatitis/cirrhotic tissues (HBV or HCV infection) by biocomputation.
Immunologic research52(3)258-68(2012 Jun)
Wang L,Huang J,Jiang M,Lin H

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PLA2G1B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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