SPINK6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPINK6
Entrez Gene ID	404203
Full Name	serine peptidase inhibitor, Kazal type 6
Synonyms	BUSI2,UNQ844
General protein information	Preferred Names serine peptidase inhibitor, Kazal type 6 Names serine protease inhibitor Kazal-type 6 acrosin inhibitor kallikrein inhibitor protease inhibitor H
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q32
Summary	The protein encoded by this gene is a Kazal-type serine protease inhibitor that acts on kallikrein-related peptidases in the skin. Two transcript variants the same protein have been found for this gene. [provided by RefSeq, Aug 2010].
Disorder MIM:	615868

mRNA and Protein(s)

mRNA	Protein	Name
NM_205841.3	NP_995313.2	serine protease inhibitor Kazal-type 6 precursor
NM_205841.4	NP_995313.2	serine protease inhibitor Kazal-type 6 precursor
NM_001195290.1	NP_001182219.1	serine protease inhibitor Kazal-type 6 precursor

Pathways from BioSystems

Homologies

	SPINK6	XP_001103616.1
Homo sapiens (human)	SPINK6	NP_995313.2
Pan troglodytes (chimpanzee)	SPINK6	XP_001160722.1

Gene Ontology
Bibliography

Related articles in PubMed

Serine protease inhibitor kazal-type 6 inhibits tumorigenesis of human hepatocellular carcinoma cells via its extracellular action.
Ge K, Huang J, Wang W, Gu M, Dai X, Xu Y, Wu H, Li G, Lu H, Zhong J, Huang Q
Oncotarget8(4)5965-5975(2017 Jan)

SPINK6 Promotes Metastasis of Nasopharyngeal Carcinoma via Binding and Activation of Epithelial Growth Factor Receptor.
Zheng LS, Yang JP, Cao Y, Peng LX, Sun R, Xie P, Wang MY, Meng DF, Luo DH, Zou X, Chen MY, Mai HQ, Guo L, Guo X, Shao JY, Huang BJ, Zhang W, Qian CN
Cancer research77(2)579-589(2017 Jan)

Gingipains of Porphyromonas gingivalis Affect the Stability and Function of Serine Protease Inhibitor of Kazal-type 6 (SPINK6), a Tissue Inhibitor of Human Kallikreins.
Plaza K, Kalinska M, Bochenska O, Meyer-Hoffert U, Wu Z, Fischer J, Falkowski K, Sasiadek L, Bielecka E, Potempa B, Kozik A, Potempa J, Kantyka T
The Journal of biological chemistry291(36)18753-64(2016 09)

The solution structure of the kallikrein-related peptidases inhibitor SPINK6.
Jung S, Fischer J, Spudy B, Kerkow T, S?nnichsen FD, Xue L, Bonvin AM, Goettig P, Magdolen V, Meyer-Hoffert U, Gr?tzinger J
Biochemical and biophysical research communications471(1)103-8(2016 Feb)

Characterization of Spink6 in mouse skin: the conserved inhibitor of kallikrein-related peptidases is reduced by barrier injury.
Fischer J, Wu Z, Kantyka T, Sperrhacke M, Dimitrieva O, Koblyakova Y, Ahrens K, Graumann N, Baurecht H, Reiss K, Schr?der JM, Proksch E, Meyer-Hoffert U
The Journal of investigative dermatology134(5)1305-1312(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our results identified a novel EGFR-activating mechanism in which SPINK6 has a critical role in promoting nasopharyngeal carcinoma metastasis
Title: SPINK6 Promotes Metastasis of Nasopharyngeal Carcinoma via Binding and Activation of Epithelial Growth Factor Receptor.

a significant fraction of SPINK6-sensitive proteases in healthy saliva and confirmed the ability of gingipains to inactivate SPINK6 under ex vivo conditions
Title: Gingipains of Porphyromonas gingivalis Affect the Stability and Function of Serine Protease Inhibitor of Kazal-type 6 (SPINK6), a Tissue Inhibitor of Human Kallikreins.

KLKs. Thereby, beside the conserved binding mode, we identified an alternate binding mode which has so far not been observed for SPINK inhibitors.
Title: The solution structure of the kallikrein-related peptidases inhibitor SPINK6.

Our study indicates that Spink6 is a potent inhibitor of kallikrein related peptidases and involved in skin barrier function.
Title: Characterization of Spink6 in mouse skin: the conserved inhibitor of kallikrein-related peptidases is reduced by barrier injury.

Cross-linked SPINK6 might protect specific substrates such as fibronectin from kallikrein-related peptidases cleavage and contribute to the regulation of proteases in the epidermis.
Title: Cross-linking of SPINK6 by transglutaminases protects from epidermal proteases.

The following SPINK6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPINK6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28766	NM_205841.4 Latest version!	Homo sapiens serine peptidase inhibitor Kazal type 6 (SPINK6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu28766	NM_001195290.1 Latest version!	Homo sapiens serine peptidase inhibitor Kazal type 6 (SPINK6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu28766	NM_205841.3	Homo sapiens serine peptidase inhibitor, Kazal type 6 (SPINK6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPINK6 ( NM_205841.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_995313.2 Homo sapiens serine peptidase inhibitor, Kazal type 6 (SPINK6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28766
Clone ID Related Accession (Same CDS sequence)	NM_205841.3 , NM_205841.4 , NM_001195290.1
Accession Version	NM_205841.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	serine protease inhibitor Kazal-type 6 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from GQ504704.1 and AY358716.1. On Sep 1, 2010 this sequence version replaced NM_205841.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032003.1, GQ504704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACTGT CAGGCATGTT TCTGCTCCTC TCTCTGGCTC TTTTCTGCTT TTTAACAGGT 
GTCTTCAGTC AGGGAGGACA GGTTGACTGT GGTGAGTTCC AGGACCCCAA GGTCTACTGC 
ACTCGGGAAT CTAACCCACA CTGTGGCTCT GATGGCCAGA CATATGGCAA TAAATGTGCC 
TTCTGTAAGG CCATAGTGAA AAGTGGTGGA AAGATTAGCC TAAAGCATCC TGGAAAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995313.2
CDS	304..546
Translation	MKLSGMFLLLSLALFCFLTGVFSQGGQVDCGEFQDPKVYCTRESNPHCGSDGQTYGNKCAFCKAIVKSGGKISLKHPGKC

Target ORF information:

RefSeq Version	NM_205841.3
Organism	Homo sapiens(human)
Definition	Homo sapiens serine peptidase inhibitor, Kazal type 6 (SPINK6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205841.3

ORF Insert Sequence:

ATGAAACTGT CAGGCATGTT TCTGCTCCTC TCTCTGGCTC TTTTCTGCTT TTTAACAGGT 
GTCTTCAGTC AGGGAGGACA GGTTGACTGT GGTGAGTTCC AGGACCCCAA GGTCTACTGC 
ACTCGGGAAT CTAACCCACA CTGTGGCTCT GATGGCCAGA CATATGGCAA TAAATGTGCC 
TTCTGTAAGG CCATAGTGAA AAGTGGTGGA AAGATTAGCC TAAAGCATCC TGGAAAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPINK6 ( NM_205841.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_995313.2 Homo sapiens serine peptidase inhibitor Kazal type 6 (SPINK6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28766
Clone ID Related Accession (Same CDS sequence)	NM_205841.3 , NM_205841.4 , NM_001195290.1
Accession Version	NM_205841.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-01
Organism	Homo sapiens(human)
Product	serine protease inhibitor Kazal-type 6 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from GQ504704.1 and AY358716.1. On Nov 23, 2018 this sequence version replaced NM_205841.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032003.1, BG191133.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000325630.3/ ENSP00000324870.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAACTGT CAGGCATGTT TCTGCTCCTC TCTCTGGCTC TTTTCTGCTT TTTAACAGGT 
GTCTTCAGTC AGGGAGGACA GGTTGACTGT GGTGAGTTCC AGGACCCCAA GGTCTACTGC 
ACTCGGGAAT CTAACCCACA CTGTGGCTCT GATGGCCAGA CATATGGCAA TAAATGTGCC 
TTCTGTAAGG CCATAGTGAA AAGTGGTGGA AAGATTAGCC TAAAGCATCC TGGAAAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995313.2
CDS	56..298
Translation	MKLSGMFLLLSLALFCFLTGVFSQGGQVDCGEFQDPKVYCTRESNPHCGSDGQTYGNKCAFCKAIVKSGGKISLKHPGKC

Target ORF information:

RefSeq Version	NM_205841.4
Organism	Homo sapiens(human)
Definition	Homo sapiens serine peptidase inhibitor Kazal type 6 (SPINK6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205841.4

ORF Insert Sequence:

ATGAAACTGT CAGGCATGTT TCTGCTCCTC TCTCTGGCTC TTTTCTGCTT TTTAACAGGT 
GTCTTCAGTC AGGGAGGACA GGTTGACTGT GGTGAGTTCC AGGACCCCAA GGTCTACTGC 
ACTCGGGAAT CTAACCCACA CTGTGGCTCT GATGGCCAGA CATATGGCAA TAAATGTGCC 
TTCTGTAAGG CCATAGTGAA AAGTGGTGGA AAGATTAGCC TAAAGCATCC TGGAAAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPINK6 ( NM_001195290.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182219.1 Homo sapiens serine peptidase inhibitor Kazal type 6 (SPINK6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28766
Clone ID Related Accession (Same CDS sequence)	NM_205841.3 , NM_205841.4 , NM_001195290.1
Accession Version	NM_001195290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	serine protease inhibitor Kazal-type 6 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from GQ504704.1, BE122852.1 and AY358716.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BE122852.1, HY174549.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968832, SAMEA2142853 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACTGT CAGGCATGTT TCTGCTCCTC TCTCTGGCTC TTTTCTGCTT TTTAACAGGT 
GTCTTCAGTC AGGGAGGACA GGTTGACTGT GGTGAGTTCC AGGACCCCAA GGTCTACTGC 
ACTCGGGAAT CTAACCCACA CTGTGGCTCT GATGGCCAGA CATATGGCAA TAAATGTGCC 
TTCTGTAAGG CCATAGTGAA AAGTGGTGGA AAGATTAGCC TAAAGCATCC TGGAAAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182219.1
CDS	177..419
Translation	MKLSGMFLLLSLALFCFLTGVFSQGGQVDCGEFQDPKVYCTRESNPHCGSDGQTYGNKCAFCKAIVKSGGKISLKHPGKC

Target ORF information:

RefSeq Version	NM_001195290.1
Organism	Homo sapiens(human)
Definition	Homo sapiens serine peptidase inhibitor Kazal type 6 (SPINK6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195290.1

ORF Insert Sequence:

ATGAAACTGT CAGGCATGTT TCTGCTCCTC TCTCTGGCTC TTTTCTGCTT TTTAACAGGT 
GTCTTCAGTC AGGGAGGACA GGTTGACTGT GGTGAGTTCC AGGACCCCAA GGTCTACTGC 
ACTCGGGAAT CTAACCCACA CTGTGGCTCT GATGGCCAGA CATATGGCAA TAAATGTGCC 
TTCTGTAAGG CCATAGTGAA AAGTGGTGGA AAGATTAGCC TAAAGCATCC TGGAAAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Serine protease inhibitor kazal-type 6 inhibits tumorigenesis of human hepatocellular carcinoma cells via its extracellular action.
Oncotarget8(4)5965-5975(2017 Jan)
Ge K,Huang J,Wang W,Gu M,Dai X,Xu Y,Wu H,Li G,Lu H,Zhong J,Huang Q

SPINK6 Promotes Metastasis of Nasopharyngeal Carcinoma via Binding and Activation of Epithelial Growth Factor Receptor.
Cancer research77(2)579-589(2017 Jan)
Zheng LS,Yang JP,Cao Y,Peng LX,Sun R,Xie P,Wang MY,Meng DF,Luo DH,Zou X,Chen MY,Mai HQ,Guo L,Guo X,Shao JY,Huang BJ,Zhang W,Qian CN

Gingipains of Porphyromonas gingivalis Affect the Stability and Function of Serine Protease Inhibitor of Kazal-type 6 (SPINK6), a Tissue Inhibitor of Human Kallikreins.
The Journal of biological chemistry291(36)18753-64(2016 09)
Plaza K,Kalinska M,Bochenska O,Meyer-Hoffert U,Wu Z,Fischer J,Falkowski K,Sasiadek L,Bielecka E,Potempa B,Kozik A,Potempa J,Kantyka T

The solution structure of the kallikrein-related peptidases inhibitor SPINK6.
Biochemical and biophysical research communications471(1)103-8(2016 Feb)
Jung S,Fischer J,Spudy B,Kerkow T,S?nnichsen FD,Xue L,Bonvin AM,Goettig P,Magdolen V,Meyer-Hoffert U,Gr?tzinger J

Characterization of Spink6 in mouse skin: the conserved inhibitor of kallikrein-related peptidases is reduced by barrier injury.
The Journal of investigative dermatology134(5)1305-1312(2014 May)
Fischer J,Wu Z,Kantyka T,Sperrhacke M,Dimitrieva O,Koblyakova Y,Ahrens K,Graumann N,Baurecht H,Reiss K,Schr?der JM,Proksch E,Meyer-Hoffert U

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SPINK6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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