LIMK1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIMK1
Entrez Gene ID	3984
Full Name	LIM domain kinase 1
Synonyms	LIMK,LIMK-1
General protein information	Preferred Names LIM domain kinase 1 Names LIM domain kinase 1 LIM motif-containing protein kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q11.23
Summary	There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011].
Disorder MIM:	601329

mRNA and Protein(s)

mRNA	Protein	Name
NM_002314.4	NP_002305.1	LIM domain kinase 1 isoform 1
NM_001204426.1	NP_001191355.1	LIM domain kinase 1 isoform 2
NM_001204426.2	NP_001191355.1	LIM domain kinase 1 isoform 2
NM_002314.3	NP_002305.1	LIM domain kinase 1 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	LIMK1	NP_002305.1
Mus musculus (house mouse)	Limk1	NP_034847.1
Gallus gallus (chicken)	LIMK1	NP_989462.1
Xenopus tropicalis (tropical clawed frog)	limk1	NP_001072900.1
	LIMK1	XP_002803600.1
Rattus norvegicus (Norway rat)	Limk1	NP_113915.2
Pan troglodytes (chimpanzee)	LIMK1	XP_001148746.2
Canis lupus familiaris (dog)	LIMK1	XP_849646.1
Bos taurus (cattle)	LIMK1	NP_001193833.1
Drosophila melanogaster (fruit fly)	LIMK1	NP_511139.2
Danio rerio (zebrafish)	limk1a	NP_001036156.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011710	XP_320802.4

Gene Ontology
Bibliography

Related articles in PubMed

MiR-519d-3p suppresses breast cancer cell growth and motility via targeting LIM domain kinase 1.
Li D, Song H, Wu T, Xie D, Hu J, Zhao J, Shen Q, Fang L
Molecular and cellular biochemistry(2017 Nov)

c-Abl tyrosine kinase regulates neutrophil crawling behavior under fluid shear stress via Rac/PAK/LIMK/cofilin signaling axis.
Tong H, Qi D, Guan X, Jiang G, Liao Z, Zhang X, Chen P, Li N, Wu M
Journal of cellular biochemistry(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LIMK1 expression is negatively regulated in granular layers-negative psoriatic epidermis or IL-22/IL-24-treated hyperproliferative reconstituted epidermis. These findings suggest a novel regulatory mechanism and a potent role of LIMK1 in psoriatic epidermis.
Title: Aberrant LIM-kinase 1 expression in hyperproliferative psoriatic epidermis.

Therefore, miR-138/LIMK1/cofilin may be considered a potential therapeutic target for the treatment of non-small cell lung cancer
Title: MicroRNA-138 inhibits migration and invasion of non-small cell lung cancer cells by targeting LIMK1.

increased BMPR2 signal transduction is linked to fragile X syndrome (FXS) and that the BMPR2-LIMK1 pathway is a putative therapeutic target in patients with FXS and possibly other forms of autism
Title: Augmented noncanonical BMP type II receptor signaling mediates the synaptic abnormality of fragile X syndrome.

Report structural basis for noncanonical substrate recognition of cofilin-1/LIMK1 to regulation actin cytoskeleton dynamics.
Title: Structural Basis for Noncanonical Substrate Recognition of Cofilin/ADF Proteins by LIM Kinases.

In turn, LIMK1 and LIMK2 are required for MT1-MMP-dependent matrix degradation and cell invasion in a three-dimensional type I collagen environment.
Title: LIMK Regulates Tumor-Cell Invasion and Matrix Degradation Through Tyrosine Phosphorylation of MT1-MMP.

The following LIMK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16673	NM_001204426.2 Latest version!	Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu22318	NM_002314.4 Latest version!	Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00
View Old Accession Versions >>
OHu16673	NM_001204426.1	Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu22318	NM_002314.3	Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIMK1 ( NM_001204426.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191355.1 Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16673
Clone ID Related Accession (Same CDS sequence)	NM_001204426.1 , NM_001204426.2
Accession Version	NM_001204426.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	LIM domain kinase 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC296332.1, AK300382.1, D26309.1 and AW016257.1. Transcript Variant: This variant (2) lacks multiple exons at the 5' end and uses a different start codon, compared to variant 1. This variant encodes an isoform (2) with a shorter and distinct N-terminus that lacks one of two LIM zinc-binding domains, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300382.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTTGG CTTCAGCCCC AAGAAGACGC CGCTTCCTCC AGAGGGCTAA GTGTTGTGAC 
TGCAGTGCCT CCCTGTCGCA CCAGTACTAT GAGAAGGATG GGCAGCTCTT CTGCAAGAAG 
GACTACTGGG CCCGCTATGG CGAGTCCTGC CATGGGTGCT CTGAGCAAAT CACCAAGGGA 
CTGGTTATGG TGGCTGGGGA GCTGAAGTAC CACCCCGAGT GTTTCATCTG CCTCACGTGT 
GGGACCTTTA TCGGTGACGG GGACACCTAC ACGCTGGTGG AGCACTCCAA GCTGTACTGC 
GGGCACTGCT ACTACCAGAC TGTGGTGACC CCCGTCATCG AGCAGATCCT GCCTGACTCC 
CCTGGCTCCC ACCTGCCCCA CACCGTCACC CTGGTGTCCA TCCCAGCCTC ATCTCATGGC 
AAGCGTGGAC TTTCAGTCTC CATTGACCCC CCGCACGGCC CACCGGGCTG TGGCACCGAG 
CACTCACACA CCGTCCGCGT CCAGGGAGTG GATCCGGGCT GCATGAGCCC AGATGTGAAG 
AATTCCATCC ACGTCGGAGA CCGGATCTTG GAAATCAATG GCACGCCCAT CCGAAATGTG 
CCCCTGGACG AGATTGACCT GCTGATTCAG GAAACCAGCC GCCTGCTCCA GCTGACCCTC 
GAGCATGACC CTCACGATAC ACTGGGCCAC GGGCTGGGGC CTGAGACCAG CCCCCTGAGC 
TCTCCGGCTT ATACTCCCAG CGGGGAGGCG GGCAGCTCTG CCCGGCAGAA ACCTGTCTTG 
AGGAGCTGCA GCATCGACAG GTCTCCGGGC GCTGGCTCAC TGGGCTCCCC GGCCTCCCAG 
CGCAAGGACC TGGGTCGCTC TGAGTCCCTC CGCGTAGTCT GCCGGCCACA CCGCATCTTC 
CGGCCGTCGG ACCTCATCCA CGGGGAGGTG CTGGGCAAGG GCTGCTTCGG CCAGGCTATC 
AAGGTGACAC ACCGTGAGAC AGGTGAGGTG ATGGTGATGA AGGAGCTGAT CCGGTTCGAC 
GAGGAGACCC AGAGGACGTT CCTCAAGGAG GTGAAGGTCA TGCGATGCCT GGAACACCCC 
AACGTGCTCA AGTTCATCGG GGTGCTCTAC AAGGACAAGA GGCTCAACTT CATCACTGAG 
TACATCAAGG GCGGCACGCT CCGGGGCATC ATCAAGAGCA TGGACAGCCA GTACCCATGG 
AGCCAGAGAG TGAGCTTTGC CAAGGACATC GCATCAGGGA TGGCCTACCT CCACTCCATG 
AACATCATCC ACCGAGACCT CAACTCCCAC AACTGCCTGG TCCGCGAGAA CAAGAATGTG 
GTGGTGGCTG ACTTCGGGCT GGCGCGTCTC ATGGTGGACG AGAAGACTCA GCCTGAGGGC 
CTGCGGAGCC TCAAGAAGCC AGACCGCAAG AAGCGCTACA CCGTGGTGGG CAACCCCTAC 
TGGATGGCAC CTGAGATGAT CAACGGCCGC AGCTATGATG AGAAGGTGGA TGTGTTCTCC 
TTTGGGATCG TCCTGTGCGA GATCATCGGG CGGGTGAACG CAGACCCTGA CTACCTGCCC 
CGCACCATGG ACTTTGGCCT CAACGTGCGA GGATTCCTGG ACCGCTACTG CCCCCCAAAC 
TGCCCCCCGA GCTTCTTCCC CATCACCGTG CGCTGTTGCG ATCTGGACCC CGAGAAGAGG 
CCATCCTTTG TGAAGCTGGA ACACTGGCTG GAGACCCTCC GCATGCACCT GGCCGGCCAC 
CTGCCACTGG GCCCACAGCT GGAGCAGCTG GACAGAGGTT TCTGGGAGAC CTACCGGCGC 
GGCGAGAGCG GACTGCCTGC CCACCCTGAG GTCCCCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191355.1
CDS	95..1936
Translation	MLLASAPRRRRFLQRAKCCDCSASLSHQYYEKDGQLFCKKDYWARYGESCHGCSEQITKGLVMVAGELKYHPECFICLTCGTFIGDGDTYTLVEHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLPHTVTLVSIPASSHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIHVGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDTLGHGLGPETSPLSSPAYTPSGEAGSSARQKPVLRSCSIDRSPGAGSLGSPASQRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLMVDEKTQPEGLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD

Target ORF information:

RefSeq Version	NM_001204426.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204426.1

ORF Insert Sequence:

ATGCTGTTGG CTTCAGCCCC AAGAAGACGC CGCTTCCTCC AGAGGGCTAA GTGTTGTGAC 
TGCAGTGCCT CCCTGTCGCA CCAGTACTAT GAGAAGGATG GGCAGCTCTT CTGCAAGAAG 
GACTACTGGG CCCGCTATGG CGAGTCCTGC CATGGGTGCT CTGAGCAAAT CACCAAGGGA 
CTGGTTATGG TGGCTGGGGA GCTGAAGTAC CACCCCGAGT GTTTCATCTG CCTCACGTGT 
GGGACCTTTA TCGGTGACGG GGACACCTAC ACGCTGGTGG AGCACTCCAA GCTGTACTGC 
GGGCACTGCT ACTACCAGAC TGTGGTGACC CCCGTCATCG AGCAGATCCT GCCTGACTCC 
CCTGGCTCCC ACCTGCCCCA CACCGTCACC CTGGTGTCCA TCCCAGCCTC ATCTCATGGC 
AAGCGTGGAC TTTCAGTCTC CATTGACCCC CCGCACGGCC CACCGGGCTG TGGCACCGAG 
CACTCACACA CCGTCCGCGT CCAGGGAGTG GATCCGGGCT GCATGAGCCC AGATGTGAAG 
AATTCCATCC ACGTCGGAGA CCGGATCTTG GAAATCAATG GCACGCCCAT CCGAAATGTG 
CCCCTGGACG AGATTGACCT GCTGATTCAG GAAACCAGCC GCCTGCTCCA GCTGACCCTC 
GAGCATGACC CTCACGATAC ACTGGGCCAC GGGCTGGGGC CTGAGACCAG CCCCCTGAGC 
TCTCCGGCTT ATACTCCCAG CGGGGAGGCG GGCAGCTCTG CCCGGCAGAA ACCTGTCTTG 
AGGAGCTGCA GCATCGACAG GTCTCCGGGC GCTGGCTCAC TGGGCTCCCC GGCCTCCCAG 
CGCAAGGACC TGGGTCGCTC TGAGTCCCTC CGCGTAGTCT GCCGGCCACA CCGCATCTTC 
CGGCCGTCGG ACCTCATCCA CGGGGAGGTG CTGGGCAAGG GCTGCTTCGG CCAGGCTATC 
AAGGTGACAC ACCGTGAGAC AGGTGAGGTG ATGGTGATGA AGGAGCTGAT CCGGTTCGAC 
GAGGAGACCC AGAGGACGTT CCTCAAGGAG GTGAAGGTCA TGCGATGCCT GGAACACCCC 
AACGTGCTCA AGTTCATCGG GGTGCTCTAC AAGGACAAGA GGCTCAACTT CATCACTGAG 
TACATCAAGG GCGGCACGCT CCGGGGCATC ATCAAGAGCA TGGACAGCCA GTACCCATGG 
AGCCAGAGAG TGAGCTTTGC CAAGGACATC GCATCAGGGA TGGCCTACCT CCACTCCATG 
AACATCATCC ACCGAGACCT CAACTCCCAC AACTGCCTGG TCCGCGAGAA CAAGAATGTG 
GTGGTGGCTG ACTTCGGGCT GGCGCGTCTC ATGGTGGACG AGAAGACTCA GCCTGAGGGC 
CTGCGGAGCC TCAAGAAGCC AGACCGCAAG AAGCGCTACA CCGTGGTGGG CAACCCCTAC 
TGGATGGCAC CTGAGATGAT CAACGGCCGC AGCTATGATG AGAAGGTGGA TGTGTTCTCC 
TTTGGGATCG TCCTGTGCGA GATCATCGGG CGGGTGAACG CAGACCCTGA CTACCTGCCC 
CGCACCATGG ACTTTGGCCT CAACGTGCGA GGATTCCTGG ACCGCTACTG CCCCCCAAAC 
TGCCCCCCGA GCTTCTTCCC CATCACCGTG CGCTGTTGCG ATCTGGACCC CGAGAAGAGG 
CCATCCTTTG TGAAGCTGGA ACACTGGCTG GAGACCCTCC GCATGCACCT GGCCGGCCAC 
CTGCCACTGG GCCCACAGCT GGAGCAGCTG GACAGAGGTT TCTGGGAGAC CTACCGGCGC 
GGCGAGAGCG GACTGCCTGC CCACCCTGAG GTCCCCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMK1 ( NM_001204426.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191355.1 Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16673
Clone ID Related Accession (Same CDS sequence)	NM_001204426.1 , NM_001204426.2
Accession Version	NM_001204426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	LIM domain kinase 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300382.1, D26309.1 and AW016257.1. On May 31, 2019 this sequence version replaced NM_001204426.1. Transcript Variant: This variant (2) lacks multiple exons at the 5' end and uses a different start codon, compared to variant 1. This variant encodes an isoform (2) with a shorter and distinct N-terminus that lacks one of two LIM zinc-binding domains, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300382.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTTGG CTTCAGCCCC AAGAAGACGC CGCTTCCTCC AGAGGGCTAA GTGTTGTGAC 
TGCAGTGCCT CCCTGTCGCA CCAGTACTAT GAGAAGGATG GGCAGCTCTT CTGCAAGAAG 
GACTACTGGG CCCGCTATGG CGAGTCCTGC CATGGGTGCT CTGAGCAAAT CACCAAGGGA 
CTGGTTATGG TGGCTGGGGA GCTGAAGTAC CACCCCGAGT GTTTCATCTG CCTCACGTGT 
GGGACCTTTA TCGGTGACGG GGACACCTAC ACGCTGGTGG AGCACTCCAA GCTGTACTGC 
GGGCACTGCT ACTACCAGAC TGTGGTGACC CCCGTCATCG AGCAGATCCT GCCTGACTCC 
CCTGGCTCCC ACCTGCCCCA CACCGTCACC CTGGTGTCCA TCCCAGCCTC ATCTCATGGC 
AAGCGTGGAC TTTCAGTCTC CATTGACCCC CCGCACGGCC CACCGGGCTG TGGCACCGAG 
CACTCACACA CCGTCCGCGT CCAGGGAGTG GATCCGGGCT GCATGAGCCC AGATGTGAAG 
AATTCCATCC ACGTCGGAGA CCGGATCTTG GAAATCAATG GCACGCCCAT CCGAAATGTG 
CCCCTGGACG AGATTGACCT GCTGATTCAG GAAACCAGCC GCCTGCTCCA GCTGACCCTC 
GAGCATGACC CTCACGATAC ACTGGGCCAC GGGCTGGGGC CTGAGACCAG CCCCCTGAGC 
TCTCCGGCTT ATACTCCCAG CGGGGAGGCG GGCAGCTCTG CCCGGCAGAA ACCTGTCTTG 
AGGAGCTGCA GCATCGACAG GTCTCCGGGC GCTGGCTCAC TGGGCTCCCC GGCCTCCCAG 
CGCAAGGACC TGGGTCGCTC TGAGTCCCTC CGCGTAGTCT GCCGGCCACA CCGCATCTTC 
CGGCCGTCGG ACCTCATCCA CGGGGAGGTG CTGGGCAAGG GCTGCTTCGG CCAGGCTATC 
AAGGTGACAC ACCGTGAGAC AGGTGAGGTG ATGGTGATGA AGGAGCTGAT CCGGTTCGAC 
GAGGAGACCC AGAGGACGTT CCTCAAGGAG GTGAAGGTCA TGCGATGCCT GGAACACCCC 
AACGTGCTCA AGTTCATCGG GGTGCTCTAC AAGGACAAGA GGCTCAACTT CATCACTGAG 
TACATCAAGG GCGGCACGCT CCGGGGCATC ATCAAGAGCA TGGACAGCCA GTACCCATGG 
AGCCAGAGAG TGAGCTTTGC CAAGGACATC GCATCAGGGA TGGCCTACCT CCACTCCATG 
AACATCATCC ACCGAGACCT CAACTCCCAC AACTGCCTGG TCCGCGAGAA CAAGAATGTG 
GTGGTGGCTG ACTTCGGGCT GGCGCGTCTC ATGGTGGACG AGAAGACTCA GCCTGAGGGC 
CTGCGGAGCC TCAAGAAGCC AGACCGCAAG AAGCGCTACA CCGTGGTGGG CAACCCCTAC 
TGGATGGCAC CTGAGATGAT CAACGGCCGC AGCTATGATG AGAAGGTGGA TGTGTTCTCC 
TTTGGGATCG TCCTGTGCGA GATCATCGGG CGGGTGAACG CAGACCCTGA CTACCTGCCC 
CGCACCATGG ACTTTGGCCT CAACGTGCGA GGATTCCTGG ACCGCTACTG CCCCCCAAAC 
TGCCCCCCGA GCTTCTTCCC CATCACCGTG CGCTGTTGCG ATCTGGACCC CGAGAAGAGG 
CCATCCTTTG TGAAGCTGGA ACACTGGCTG GAGACCCTCC GCATGCACCT GGCCGGCCAC 
CTGCCACTGG GCCCACAGCT GGAGCAGCTG GACAGAGGTT TCTGGGAGAC CTACCGGCGC 
GGCGAGAGCG GACTGCCTGC CCACCCTGAG GTCCCCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191355.1
CDS	34..1875
Translation	MLLASAPRRRRFLQRAKCCDCSASLSHQYYEKDGQLFCKKDYWARYGESCHGCSEQITKGLVMVAGELKYHPECFICLTCGTFIGDGDTYTLVEHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLPHTVTLVSIPASSHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIHVGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDTLGHGLGPETSPLSSPAYTPSGEAGSSARQKPVLRSCSIDRSPGAGSLGSPASQRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLMVDEKTQPEGLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD

Target ORF information:

RefSeq Version	NM_001204426.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204426.2

ORF Insert Sequence:

ATGCTGTTGG CTTCAGCCCC AAGAAGACGC CGCTTCCTCC AGAGGGCTAA GTGTTGTGAC 
TGCAGTGCCT CCCTGTCGCA CCAGTACTAT GAGAAGGATG GGCAGCTCTT CTGCAAGAAG 
GACTACTGGG CCCGCTATGG CGAGTCCTGC CATGGGTGCT CTGAGCAAAT CACCAAGGGA 
CTGGTTATGG TGGCTGGGGA GCTGAAGTAC CACCCCGAGT GTTTCATCTG CCTCACGTGT 
GGGACCTTTA TCGGTGACGG GGACACCTAC ACGCTGGTGG AGCACTCCAA GCTGTACTGC 
GGGCACTGCT ACTACCAGAC TGTGGTGACC CCCGTCATCG AGCAGATCCT GCCTGACTCC 
CCTGGCTCCC ACCTGCCCCA CACCGTCACC CTGGTGTCCA TCCCAGCCTC ATCTCATGGC 
AAGCGTGGAC TTTCAGTCTC CATTGACCCC CCGCACGGCC CACCGGGCTG TGGCACCGAG 
CACTCACACA CCGTCCGCGT CCAGGGAGTG GATCCGGGCT GCATGAGCCC AGATGTGAAG 
AATTCCATCC ACGTCGGAGA CCGGATCTTG GAAATCAATG GCACGCCCAT CCGAAATGTG 
CCCCTGGACG AGATTGACCT GCTGATTCAG GAAACCAGCC GCCTGCTCCA GCTGACCCTC 
GAGCATGACC CTCACGATAC ACTGGGCCAC GGGCTGGGGC CTGAGACCAG CCCCCTGAGC 
TCTCCGGCTT ATACTCCCAG CGGGGAGGCG GGCAGCTCTG CCCGGCAGAA ACCTGTCTTG 
AGGAGCTGCA GCATCGACAG GTCTCCGGGC GCTGGCTCAC TGGGCTCCCC GGCCTCCCAG 
CGCAAGGACC TGGGTCGCTC TGAGTCCCTC CGCGTAGTCT GCCGGCCACA CCGCATCTTC 
CGGCCGTCGG ACCTCATCCA CGGGGAGGTG CTGGGCAAGG GCTGCTTCGG CCAGGCTATC 
AAGGTGACAC ACCGTGAGAC AGGTGAGGTG ATGGTGATGA AGGAGCTGAT CCGGTTCGAC 
GAGGAGACCC AGAGGACGTT CCTCAAGGAG GTGAAGGTCA TGCGATGCCT GGAACACCCC 
AACGTGCTCA AGTTCATCGG GGTGCTCTAC AAGGACAAGA GGCTCAACTT CATCACTGAG 
TACATCAAGG GCGGCACGCT CCGGGGCATC ATCAAGAGCA TGGACAGCCA GTACCCATGG 
AGCCAGAGAG TGAGCTTTGC CAAGGACATC GCATCAGGGA TGGCCTACCT CCACTCCATG 
AACATCATCC ACCGAGACCT CAACTCCCAC AACTGCCTGG TCCGCGAGAA CAAGAATGTG 
GTGGTGGCTG ACTTCGGGCT GGCGCGTCTC ATGGTGGACG AGAAGACTCA GCCTGAGGGC 
CTGCGGAGCC TCAAGAAGCC AGACCGCAAG AAGCGCTACA CCGTGGTGGG CAACCCCTAC 
TGGATGGCAC CTGAGATGAT CAACGGCCGC AGCTATGATG AGAAGGTGGA TGTGTTCTCC 
TTTGGGATCG TCCTGTGCGA GATCATCGGG CGGGTGAACG CAGACCCTGA CTACCTGCCC 
CGCACCATGG ACTTTGGCCT CAACGTGCGA GGATTCCTGG ACCGCTACTG CCCCCCAAAC 
TGCCCCCCGA GCTTCTTCCC CATCACCGTG CGCTGTTGCG ATCTGGACCC CGAGAAGAGG 
CCATCCTTTG TGAAGCTGGA ACACTGGCTG GAGACCCTCC GCATGCACCT GGCCGGCCAC 
CTGCCACTGG GCCCACAGCT GGAGCAGCTG GACAGAGGTT TCTGGGAGAC CTACCGGCGC 
GGCGAGAGCG GACTGCCTGC CCACCCTGAG GTCCCCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMK1 ( NM_002314.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002305.1 Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22318
Clone ID Related Accession (Same CDS sequence)	NM_002314.4 , NM_002314.3
Accession Version	NM_002314.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	LIM domain kinase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005056.2, D26309.1 and AW016257.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002314.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. The exon structure of the 5' UTR has been inferred from cross-species comparisons and lacks direct experimental support. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.8350.1, SRR1660805.38201.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000336180.7/ ENSP00000336740.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGTTGA CGCTACTTTG TTGCACCTGG AGGGAAGAAC GTATGGGAGA GGAAGGAAGC 
GAGTTGCCCG TGTGTGCAAG CTGCGGCCAG AGGATCTATG ATGGCCAGTA CCTCCAGGCC 
CTGAACGCGG ACTGGCACGC AGACTGCTTC AGGTGTTGTG ACTGCAGTGC CTCCCTGTCG 
CACCAGTACT ATGAGAAGGA TGGGCAGCTC TTCTGCAAGA AGGACTACTG GGCCCGCTAT 
GGCGAGTCCT GCCATGGGTG CTCTGAGCAA ATCACCAAGG GACTGGTTAT GGTGGCTGGG 
GAGCTGAAGT ACCACCCCGA GTGTTTCATC TGCCTCACGT GTGGGACCTT TATCGGTGAC 
GGGGACACCT ACACGCTGGT GGAGCACTCC AAGCTGTACT GCGGGCACTG CTACTACCAG 
ACTGTGGTGA CCCCCGTCAT CGAGCAGATC CTGCCTGACT CCCCTGGCTC CCACCTGCCC 
CACACCGTCA CCCTGGTGTC CATCCCAGCC TCATCTCATG GCAAGCGTGG ACTTTCAGTC 
TCCATTGACC CCCCGCACGG CCCACCGGGC TGTGGCACCG AGCACTCACA CACCGTCCGC 
GTCCAGGGAG TGGATCCGGG CTGCATGAGC CCAGATGTGA AGAATTCCAT CCACGTCGGA 
GACCGGATCT TGGAAATCAA TGGCACGCCC ATCCGAAATG TGCCCCTGGA CGAGATTGAC 
CTGCTGATTC AGGAAACCAG CCGCCTGCTC CAGCTGACCC TCGAGCATGA CCCTCACGAT 
ACACTGGGCC ACGGGCTGGG GCCTGAGACC AGCCCCCTGA GCTCTCCGGC TTATACTCCC 
AGCGGGGAGG CGGGCAGCTC TGCCCGGCAG AAACCTGTCT TGAGGAGCTG CAGCATCGAC 
AGGTCTCCGG GCGCTGGCTC ACTGGGCTCC CCGGCCTCCC AGCGCAAGGA CCTGGGTCGC 
TCTGAGTCCC TCCGCGTAGT CTGCCGGCCA CACCGCATCT TCCGGCCGTC GGACCTCATC 
CACGGGGAGG TGCTGGGCAA GGGCTGCTTC GGCCAGGCTA TCAAGGTGAC ACACCGTGAG 
ACAGGTGAGG TGATGGTGAT GAAGGAGCTG ATCCGGTTCG ACGAGGAGAC CCAGAGGACG 
TTCCTCAAGG AGGTGAAGGT CATGCGATGC CTGGAACACC CCAACGTGCT CAAGTTCATC 
GGGGTGCTCT ACAAGGACAA GAGGCTCAAC TTCATCACTG AGTACATCAA GGGCGGCACG 
CTCCGGGGCA TCATCAAGAG CATGGACAGC CAGTACCCAT GGAGCCAGAG AGTGAGCTTT 
GCCAAGGACA TCGCATCAGG GATGGCCTAC CTCCACTCCA TGAACATCAT CCACCGAGAC 
CTCAACTCCC ACAACTGCCT GGTCCGCGAG AACAAGAATG TGGTGGTGGC TGACTTCGGG 
CTGGCGCGTC TCATGGTGGA CGAGAAGACT CAGCCTGAGG GCCTGCGGAG CCTCAAGAAG 
CCAGACCGCA AGAAGCGCTA CACCGTGGTG GGCAACCCCT ACTGGATGGC ACCTGAGATG 
ATCAACGGCC GCAGCTATGA TGAGAAGGTG GATGTGTTCT CCTTTGGGAT CGTCCTGTGC 
GAGATCATCG GGCGGGTGAA CGCAGACCCT GACTACCTGC CCCGCACCAT GGACTTTGGC 
CTCAACGTGC GAGGATTCCT GGACCGCTAC TGCCCCCCAA ACTGCCCCCC GAGCTTCTTC 
CCCATCACCG TGCGCTGTTG CGATCTGGAC CCCGAGAAGA GGCCATCCTT TGTGAAGCTG 
GAACACTGGC TGGAGACCCT CCGCATGCAC CTGGCCGGCC ACCTGCCACT GGGCCCACAG 
CTGGAGCAGC TGGACAGAGG TTTCTGGGAG ACCTACCGGC GCGGCGAGAG CGGACTGCCT 
GCCCACCCTG AGGTCCCCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002305.1
CDS	188..2131
Translation	MRLTLLCCTWREERMGEEGSELPVCASCGQRIYDGQYLQALNADWHADCFRCCDCSASLSHQYYEKDGQLFCKKDYWARYGESCHGCSEQITKGLVMVAGELKYHPECFICLTCGTFIGDGDTYTLVEHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLPHTVTLVSIPASSHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIHVGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDTLGHGLGPETSPLSSPAYTPSGEAGSSARQKPVLRSCSIDRSPGAGSLGSPASQRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLMVDEKTQPEGLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD

Target ORF information:

RefSeq Version	NM_002314.4
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002314.4

ORF Insert Sequence:

ATGAGGTTGA CGCTACTTTG TTGCACCTGG AGGGAAGAAC GTATGGGAGA GGAAGGAAGC 
GAGTTGCCCG TGTGTGCAAG CTGCGGCCAG AGGATCTATG ATGGCCAGTA CCTCCAGGCC 
CTGAACGCGG ACTGGCACGC AGACTGCTTC AGGTGTTGTG ACTGCAGTGC CTCCCTGTCG 
CACCAGTACT ATGAGAAGGA TGGGCAGCTC TTCTGCAAGA AGGACTACTG GGCCCGCTAT 
GGCGAGTCCT GCCATGGGTG CTCTGAGCAA ATCACCAAGG GACTGGTTAT GGTGGCTGGG 
GAGCTGAAGT ACCACCCCGA GTGTTTCATC TGCCTCACGT GTGGGACCTT TATCGGTGAC 
GGGGACACCT ACACGCTGGT GGAGCACTCC AAGCTGTACT GCGGGCACTG CTACTACCAG 
ACTGTGGTGA CCCCCGTCAT CGAGCAGATC CTGCCTGACT CCCCTGGCTC CCACCTGCCC 
CACACCGTCA CCCTGGTGTC CATCCCAGCC TCATCTCATG GCAAGCGTGG ACTTTCAGTC 
TCCATTGACC CCCCGCACGG CCCACCGGGC TGTGGCACCG AGCACTCACA CACCGTCCGC 
GTCCAGGGAG TGGATCCGGG CTGCATGAGC CCAGATGTGA AGAATTCCAT CCACGTCGGA 
GACCGGATCT TGGAAATCAA TGGCACGCCC ATCCGAAATG TGCCCCTGGA CGAGATTGAC 
CTGCTGATTC AGGAAACCAG CCGCCTGCTC CAGCTGACCC TCGAGCATGA CCCTCACGAT 
ACACTGGGCC ACGGGCTGGG GCCTGAGACC AGCCCCCTGA GCTCTCCGGC TTATACTCCC 
AGCGGGGAGG CGGGCAGCTC TGCCCGGCAG AAACCTGTCT TGAGGAGCTG CAGCATCGAC 
AGGTCTCCGG GCGCTGGCTC ACTGGGCTCC CCGGCCTCCC AGCGCAAGGA CCTGGGTCGC 
TCTGAGTCCC TCCGCGTAGT CTGCCGGCCA CACCGCATCT TCCGGCCGTC GGACCTCATC 
CACGGGGAGG TGCTGGGCAA GGGCTGCTTC GGCCAGGCTA TCAAGGTGAC ACACCGTGAG 
ACAGGTGAGG TGATGGTGAT GAAGGAGCTG ATCCGGTTCG ACGAGGAGAC CCAGAGGACG 
TTCCTCAAGG AGGTGAAGGT CATGCGATGC CTGGAACACC CCAACGTGCT CAAGTTCATC 
GGGGTGCTCT ACAAGGACAA GAGGCTCAAC TTCATCACTG AGTACATCAA GGGCGGCACG 
CTCCGGGGCA TCATCAAGAG CATGGACAGC CAGTACCCAT GGAGCCAGAG AGTGAGCTTT 
GCCAAGGACA TCGCATCAGG GATGGCCTAC CTCCACTCCA TGAACATCAT CCACCGAGAC 
CTCAACTCCC ACAACTGCCT GGTCCGCGAG AACAAGAATG TGGTGGTGGC TGACTTCGGG 
CTGGCGCGTC TCATGGTGGA CGAGAAGACT CAGCCTGAGG GCCTGCGGAG CCTCAAGAAG 
CCAGACCGCA AGAAGCGCTA CACCGTGGTG GGCAACCCCT ACTGGATGGC ACCTGAGATG 
ATCAACGGCC GCAGCTATGA TGAGAAGGTG GATGTGTTCT CCTTTGGGAT CGTCCTGTGC 
GAGATCATCG GGCGGGTGAA CGCAGACCCT GACTACCTGC CCCGCACCAT GGACTTTGGC 
CTCAACGTGC GAGGATTCCT GGACCGCTAC TGCCCCCCAA ACTGCCCCCC GAGCTTCTTC 
CCCATCACCG TGCGCTGTTG CGATCTGGAC CCCGAGAAGA GGCCATCCTT TGTGAAGCTG 
GAACACTGGC TGGAGACCCT CCGCATGCAC CTGGCCGGCC ACCTGCCACT GGGCCCACAG 
CTGGAGCAGC TGGACAGAGG TTTCTGGGAG ACCTACCGGC GCGGCGAGAG CGGACTGCCT 
GCCCACCCTG AGGTCCCCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMK1 ( NM_002314.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002305.1 Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA.

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CloneID	OHu22318
Clone ID Related Accession (Same CDS sequence)	NM_002314.4 , NM_002314.3
Accession Version	NM_002314.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	LIM domain kinase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005056.2, D26309.1 and AW016257.1. This sequence is a reference standard in the RefSeqGene project. On Mar 1, 2011 this sequence version replaced NM_002314.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. The exon structure of the 5' UTR has been inferred from cross-species comparisons and lacks direct experimental support. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.8350.1, SRR1660805.38201.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGGTTGA CGCTACTTTG TTGCACCTGG AGGGAAGAAC GTATGGGAGA GGAAGGAAGC 
GAGTTGCCCG TGTGTGCAAG CTGCGGCCAG AGGATCTATG ATGGCCAGTA CCTCCAGGCC 
CTGAACGCGG ACTGGCACGC AGACTGCTTC AGGTGTTGTG ACTGCAGTGC CTCCCTGTCG 
CACCAGTACT ATGAGAAGGA TGGGCAGCTC TTCTGCAAGA AGGACTACTG GGCCCGCTAT 
GGCGAGTCCT GCCATGGGTG CTCTGAGCAA ATCACCAAGG GACTGGTTAT GGTGGCTGGG 
GAGCTGAAGT ACCACCCCGA GTGTTTCATC TGCCTCACGT GTGGGACCTT TATCGGTGAC 
GGGGACACCT ACACGCTGGT GGAGCACTCC AAGCTGTACT GCGGGCACTG CTACTACCAG 
ACTGTGGTGA CCCCCGTCAT CGAGCAGATC CTGCCTGACT CCCCTGGCTC CCACCTGCCC 
CACACCGTCA CCCTGGTGTC CATCCCAGCC TCATCTCATG GCAAGCGTGG ACTTTCAGTC 
TCCATTGACC CCCCGCACGG CCCACCGGGC TGTGGCACCG AGCACTCACA CACCGTCCGC 
GTCCAGGGAG TGGATCCGGG CTGCATGAGC CCAGATGTGA AGAATTCCAT CCACGTCGGA 
GACCGGATCT TGGAAATCAA TGGCACGCCC ATCCGAAATG TGCCCCTGGA CGAGATTGAC 
CTGCTGATTC AGGAAACCAG CCGCCTGCTC CAGCTGACCC TCGAGCATGA CCCTCACGAT 
ACACTGGGCC ACGGGCTGGG GCCTGAGACC AGCCCCCTGA GCTCTCCGGC TTATACTCCC 
AGCGGGGAGG CGGGCAGCTC TGCCCGGCAG AAACCTGTCT TGAGGAGCTG CAGCATCGAC 
AGGTCTCCGG GCGCTGGCTC ACTGGGCTCC CCGGCCTCCC AGCGCAAGGA CCTGGGTCGC 
TCTGAGTCCC TCCGCGTAGT CTGCCGGCCA CACCGCATCT TCCGGCCGTC GGACCTCATC 
CACGGGGAGG TGCTGGGCAA GGGCTGCTTC GGCCAGGCTA TCAAGGTGAC ACACCGTGAG 
ACAGGTGAGG TGATGGTGAT GAAGGAGCTG ATCCGGTTCG ACGAGGAGAC CCAGAGGACG 
TTCCTCAAGG AGGTGAAGGT CATGCGATGC CTGGAACACC CCAACGTGCT CAAGTTCATC 
GGGGTGCTCT ACAAGGACAA GAGGCTCAAC TTCATCACTG AGTACATCAA GGGCGGCACG 
CTCCGGGGCA TCATCAAGAG CATGGACAGC CAGTACCCAT GGAGCCAGAG AGTGAGCTTT 
GCCAAGGACA TCGCATCAGG GATGGCCTAC CTCCACTCCA TGAACATCAT CCACCGAGAC 
CTCAACTCCC ACAACTGCCT GGTCCGCGAG AACAAGAATG TGGTGGTGGC TGACTTCGGG 
CTGGCGCGTC TCATGGTGGA CGAGAAGACT CAGCCTGAGG GCCTGCGGAG CCTCAAGAAG 
CCAGACCGCA AGAAGCGCTA CACCGTGGTG GGCAACCCCT ACTGGATGGC ACCTGAGATG 
ATCAACGGCC GCAGCTATGA TGAGAAGGTG GATGTGTTCT CCTTTGGGAT CGTCCTGTGC 
GAGATCATCG GGCGGGTGAA CGCAGACCCT GACTACCTGC CCCGCACCAT GGACTTTGGC 
CTCAACGTGC GAGGATTCCT GGACCGCTAC TGCCCCCCAA ACTGCCCCCC GAGCTTCTTC 
CCCATCACCG TGCGCTGTTG CGATCTGGAC CCCGAGAAGA GGCCATCCTT TGTGAAGCTG 
GAACACTGGC TGGAGACCCT CCGCATGCAC CTGGCCGGCC ACCTGCCACT GGGCCCACAG 
CTGGAGCAGC TGGACAGAGG TTTCTGGGAG ACCTACCGGC GCGGCGAGAG CGGACTGCCT 
GCCCACCCTG AGGTCCCCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002305.1
CDS	215..2158
Translation	MRLTLLCCTWREERMGEEGSELPVCASCGQRIYDGQYLQALNADWHADCFRCCDCSASLSHQYYEKDGQLFCKKDYWARYGESCHGCSEQITKGLVMVAGELKYHPECFICLTCGTFIGDGDTYTLVEHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLPHTVTLVSIPASSHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIHVGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDTLGHGLGPETSPLSSPAYTPSGEAGSSARQKPVLRSCSIDRSPGAGSLGSPASQRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLMVDEKTQPEGLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD

Target ORF information:

RefSeq Version	NM_002314.3
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002314.3

ORF Insert Sequence:

ATGAGGTTGA CGCTACTTTG TTGCACCTGG AGGGAAGAAC GTATGGGAGA GGAAGGAAGC 
GAGTTGCCCG TGTGTGCAAG CTGCGGCCAG AGGATCTATG ATGGCCAGTA CCTCCAGGCC 
CTGAACGCGG ACTGGCACGC AGACTGCTTC AGGTGTTGTG ACTGCAGTGC CTCCCTGTCG 
CACCAGTACT ATGAGAAGGA TGGGCAGCTC TTCTGCAAGA AGGACTACTG GGCCCGCTAT 
GGCGAGTCCT GCCATGGGTG CTCTGAGCAA ATCACCAAGG GACTGGTTAT GGTGGCTGGG 
GAGCTGAAGT ACCACCCCGA GTGTTTCATC TGCCTCACGT GTGGGACCTT TATCGGTGAC 
GGGGACACCT ACACGCTGGT GGAGCACTCC AAGCTGTACT GCGGGCACTG CTACTACCAG 
ACTGTGGTGA CCCCCGTCAT CGAGCAGATC CTGCCTGACT CCCCTGGCTC CCACCTGCCC 
CACACCGTCA CCCTGGTGTC CATCCCAGCC TCATCTCATG GCAAGCGTGG ACTTTCAGTC 
TCCATTGACC CCCCGCACGG CCCACCGGGC TGTGGCACCG AGCACTCACA CACCGTCCGC 
GTCCAGGGAG TGGATCCGGG CTGCATGAGC CCAGATGTGA AGAATTCCAT CCACGTCGGA 
GACCGGATCT TGGAAATCAA TGGCACGCCC ATCCGAAATG TGCCCCTGGA CGAGATTGAC 
CTGCTGATTC AGGAAACCAG CCGCCTGCTC CAGCTGACCC TCGAGCATGA CCCTCACGAT 
ACACTGGGCC ACGGGCTGGG GCCTGAGACC AGCCCCCTGA GCTCTCCGGC TTATACTCCC 
AGCGGGGAGG CGGGCAGCTC TGCCCGGCAG AAACCTGTCT TGAGGAGCTG CAGCATCGAC 
AGGTCTCCGG GCGCTGGCTC ACTGGGCTCC CCGGCCTCCC AGCGCAAGGA CCTGGGTCGC 
TCTGAGTCCC TCCGCGTAGT CTGCCGGCCA CACCGCATCT TCCGGCCGTC GGACCTCATC 
CACGGGGAGG TGCTGGGCAA GGGCTGCTTC GGCCAGGCTA TCAAGGTGAC ACACCGTGAG 
ACAGGTGAGG TGATGGTGAT GAAGGAGCTG ATCCGGTTCG ACGAGGAGAC CCAGAGGACG 
TTCCTCAAGG AGGTGAAGGT CATGCGATGC CTGGAACACC CCAACGTGCT CAAGTTCATC 
GGGGTGCTCT ACAAGGACAA GAGGCTCAAC TTCATCACTG AGTACATCAA GGGCGGCACG 
CTCCGGGGCA TCATCAAGAG CATGGACAGC CAGTACCCAT GGAGCCAGAG AGTGAGCTTT 
GCCAAGGACA TCGCATCAGG GATGGCCTAC CTCCACTCCA TGAACATCAT CCACCGAGAC 
CTCAACTCCC ACAACTGCCT GGTCCGCGAG AACAAGAATG TGGTGGTGGC TGACTTCGGG 
CTGGCGCGTC TCATGGTGGA CGAGAAGACT CAGCCTGAGG GCCTGCGGAG CCTCAAGAAG 
CCAGACCGCA AGAAGCGCTA CACCGTGGTG GGCAACCCCT ACTGGATGGC ACCTGAGATG 
ATCAACGGCC GCAGCTATGA TGAGAAGGTG GATGTGTTCT CCTTTGGGAT CGTCCTGTGC 
GAGATCATCG GGCGGGTGAA CGCAGACCCT GACTACCTGC CCCGCACCAT GGACTTTGGC 
CTCAACGTGC GAGGATTCCT GGACCGCTAC TGCCCCCCAA ACTGCCCCCC GAGCTTCTTC 
CCCATCACCG TGCGCTGTTG CGATCTGGAC CCCGAGAAGA GGCCATCCTT TGTGAAGCTG 
GAACACTGGC TGGAGACCCT CCGCATGCAC CTGGCCGGCC ACCTGCCACT GGGCCCACAG 
CTGGAGCAGC TGGACAGAGG TTTCTGGGAG ACCTACCGGC GCGGCGAGAG CGGACTGCCT 
GCCCACCCTG AGGTCCCCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MiR-519d-3p suppresses breast cancer cell growth and motility via targeting LIM domain kinase 1.
Molecular and cellular biochemistry(2017 Nov)
Li D,Song H,Wu T,Xie D,Hu J,Zhao J,Shen Q,Fang L

c-Abl tyrosine kinase regulates neutrophil crawling behavior under fluid shear stress via Rac/PAK/LIMK/cofilin signaling axis.
Journal of cellular biochemistry(2017 Oct)
Tong H,Qi D,Guan X,Jiang G,Liao Z,Zhang X,Chen P,Li N,Wu M

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LIMK1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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