LIMK1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIMK1
Entrez Gene ID	32207
Full Name	LIM domain kinase 1
Synonyms	CG1848,D-LIMK1,D-Limk,DLIMK,DLIMK1,Dlimk,Dmel\CG1848,LIM-kinase 1,LIMK,LIMK-1,LimK,LimK1,Limk,ag,agn,dLIMK,dLIMK1,l(1)11Af,limK1,limk,limk1,ts398
General protein information	Preferred Names LIM domain kinase 1 Names CG1848 gene product from transcript CG1848-RC CG1848-PA CG1848-PC CG1848-PD CG1848-PE CG1848-PF LIM Kinase1 LIM domain protein kinase LIM kinase 1 LIM-DOMAIN KINASE LIM-kinase LIM-kinase 1 LIM-kinase1 LIMK1-PA LIMK1-PC LIMK1-PD LIMK1-PE LIMK1-PF Lim kinase agnostic im kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11A8-11A8\|1-38.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169263.1	NP_001162734.1	LIM domain kinase 1, isoform F
NM_078584.3	NP_511139.2	LIM domain kinase 1, isoform C
NM_001169262.1	NP_001162733.1	LIM domain kinase 1, isoform E
NM_167326.2	NP_727621.1	LIM domain kinase 1, isoform A
NM_167327.2	NP_727622.1	LIM domain kinase 1, isoform D

Pathways from BioSystems

Homologies

Homo sapiens (human)	LIMK1	NP_002305.1
Mus musculus (house mouse)	Limk1	NP_034847.1
Gallus gallus (chicken)	LIMK1	NP_989462.1
Xenopus tropicalis (tropical clawed frog)	limk1	NP_001072900.1
	LIMK1	XP_002803600.1
Rattus norvegicus (Norway rat)	Limk1	NP_113915.2
Pan troglodytes (chimpanzee)	LIMK1	XP_001148746.2
Canis lupus familiaris (dog)	LIMK1	XP_849646.1
Bos taurus (cattle)	LIMK1	NP_001193833.1
Drosophila melanogaster (fruit fly)	LIMK1	NP_511139.2
Danio rerio (zebrafish)	limk1a	NP_001036156.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011710	XP_320802.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic analysis in Drosophila midgut revealed a conserved role for dLIMK in constraining gastrointestinal stem cell proliferation.
Title: Reduced LIMK2 expression in colorectal cancer reflects its role in limiting stem cell proliferation.

functional connections at the behavioral level (learning/memory) depending on the GDNF and LIMK1 brain expression and HSPs transduction that might provide targets for complex approaches for the neurodegenerative diseases treatment.
Title: [Participation of GDNF, LIMK1 signal pathways and heat shock proteins in processes of Drosophila learning and memory formation].

Rac may signal through Pak and LIMK to result in uniform phosphorylation of cofilin in all the outer border cells.
Title: Regulation of cofilin phosphorylation and asymmetry in collective cell migration during morphogenesis.

The higher resistance of characterictics of behavior and communicative sound signals to heat shock is in agreement with the fact that the extremely high LIMK1 and P-cofilin concentrations in cells go down to normal values after heat shock.
Title: [Courtship behavior, communicative sound production and resistance to stress in Drosophila mutants with defective agnostic gene, coding for LIMK1].

Limk is a critical regulator of cofilin function and synapse development, and a downstream effector of p21-activated kinase (Pak) in vivo.
Title: Lim kinase regulates the development of olfactory and neuromuscular synapses.

The following LIMK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01405	NM_078584.3 Latest version!	Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa01406	NM_167327.2 Latest version!	Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa01407	NM_001169262.1 Latest version!	Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa01407	NM_167326.2 Latest version!	Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa02798	NM_001169263.1 Latest version!	Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIMK1 ( NM_078584.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511139.2 Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01405
Clone ID Related Accession (Same CDS sequence)	NM_078584.3
Accession Version	NM_078584.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	LIM domain kinase 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_078584.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCATC AGCAGCGCCT GCGGGCAAAT GGCGGACGAG GAGGCACTGG ATTGGGCGCA 
GGATCAGGAC CTGTGTCCGG AGGACACTCA CCATTGTGCG CCCATTGTCG GGGTCAACTA 
CTGCCACATC CAGAGGAGCC AATCGTGATG GCTCTCGGCC AGCAATGGCA CTGCGATTGC 
TTTCGCTGCT CCGTTTGCGA GGGCCATCTG CATAACTGGT ACTTCGAACG GGAGGGCTTA 
CTGTACTGTC GTGAGGATTA CTACGGCCGA TTCGGCGATG CCTGTCAGCA GTGTATGGCC 
GTTATCACCG GACCCGTAAT GGTCGCCGGC GAGCACAAAT TCCATCCGGA GTGCTTCTGC 
TGCACGGCAT GCGGCTCCTT CATCGGCGAG GGCGAATCGT ACGCCCTTGT CGAGCGTTCG 
AAGCTCTATT GTGGCCAGTG CTATGGCAAA CGTTCCTGCC AGCCGGCGGA TGCGAAGGCG 
CGGATCACAA CGGCCGGCAA GCCGATGCAC TCCATCCGTC TGGTCGAGAT ACCCAAGGAC 
GCCACGCCCG GCCTGAGGGT CGACGGAGTG GCCCTCGACG ATGGTTGTCC CACGGTCCGC 
ATTACAGACT TGTTCTGTAA CTTCTTCTTG TGGCTAACCT CGATGGCGGA ACAGTGCTGT 
GGGGCGCCTT ATAGAATTGA TGTGAATCTT ACAAATCTGC ATATTGGAGA TAGGATATTG 
GAGGTGAACG GCACACCAGT TAGTGATTCG TCCGTGGAAC AGATCGACAA ATTGATACGG 
AGTAATGAGA AGATGCTTCA GCTGACCGTG GAGCATGATC CGGTGCAAGT GTGCCGCTCC 
TGCAGCCAAG CGGACATCCA GCGGGCCATG TCAGCGTCCA CGTTAATCCT GCCGCTCAGC 
ACATCGGCCT CCAGTGTCGA GGTGGGCAGG GAGCGTCTGT ACAAGACACC CGGCGAACAG 
GGCACGAAGG CCAGGAAACT GCGACAAGCC ACCAATGCCT CTACGACAAT ACCACCGGCA 
GCTGGAGCCA CCGCAATGAC GCAACTAAAG GAGAAGGAGC GTTGTTCCAG TCTGTCCAAG 
TTGCTGGATG AACAGCATCA GGCACAACAG CATTCGGCGC ATCCTCAGCT CTATGATCTG 
TCCAGGACGC AATCCTGCCG TGTCGTCCAA AAGCCACAAC GAATCTTTCG TGCAACTGAT 
CTGGTTATAG GAGAGAAGCT AGGCGAGGGC TTCTTTGGCA AGGTTTTCAA GGTGACCCAC 
CGTCAGAGCG GCGAGGTGAT GGTCCTCAAA GAACTGCATC GTGCGGATGA GGAAGCGCAA 
AGGAATTTCA TCAAGGAAGT GGCCGTTCTC CGTTTGCTGG ACCATCGGCA TGTGCTCAAA 
TTCATTGGTG TTCTGTACAA GGATAAGAAA CTGCACATGG TCACGGAGTA CGTGGCCGGT 
GGTTGTCTCA AGGAGCTGAT CCACGATCCA GCCCAAGTAC TCCCTTGGCC CCAGCGCGTC 
CGCTTGGCCA GGGATATTGC GTGCGGCATG AGCTATCTGC ACTCGATGAA CATCATCCAT 
CGCGATTTGA ACTCAATGAA TTGCCTGGTC CGTGAAGATC GCTCGGTCAT AGTGGCGGAT 
TTTGGACTAG CTCGCAGTGT GGATGCGCCG CGGCTGCCAA GTGGCAATAT GACGCCAGGC 
GGCTATGGAT CAGGCGCTAA CAGCGATGCG CCCATGTCAC CGAGTGGAAC ACTAAGGCGC 
AGCAAGAGCA GGCAGCGAAG GCAACGTTAC ACGGTGGTCG GGAATCCCTA CTGGATGGCA 
CCCGAGATGA TGAAAGGTCT CAAATACGAC GAAAAGGTGG ATGTCTTCTC CTTTGGCATT 
ATGTTGTGTG AGATCATTGG ACGCGTGGAA GCCGATCCGG ATTTTATGCC TCGCAATTCG 
GACTTCAGTC TCAATCAGCA GGAGTTTCGC GAGAAGTTCT GCGCTCAGTG TCCAGAGCCT 
TTCGTTAAAG TGGCCTTCGT GTGCTGTGAT CTTAATCCCG ATATGCGTCC GTGTTTCGAA 
ACTCTGCACG TTTGGCTGCA GCGTCTCGCC GATGATCTGG CCGCCGATCG TGTGCCGCCA 
GAGCGTCTGC TGCACGAGAT TGAGACCTTT CAGGAATGGT ACGCCAGCTC TGAGGACGCC 
TTGTCACCCA CCTCCCAGCG CAGCTTAAAT AATCTCGATG AGTTGGTCAA GAGTGCGGTG 
GATTCGGAAA TTTCGCCGGT AGAAAAAGAG AAGGAGAATA TGGTGATTAA GCCCCAGGAT 
ATACCGAAAT CTCCGCATCT CGGCAAGGAT TTCTCGCCCA GCGGCGAGAG ACTGCGTGAC 
AGTATGCGGG CCAGGCGTCG CCAACGTTTC TTGGGCGCCC AGGAGGAGCG TCGTAATCTC 
ACGCCCGACA CGGAGTCCAA GGAAAGGGCT TTGAAGAAGG CGCTGAAGAA ATGTCGTCCG 
TTTGGCGAAA GAGGTTACCT GGTGGATCTG CGTGCCGGTG CCGAATTGCA GCTAGAGGAT 
GTCAGGGATT TGAACACCTA TTCCGATGTG GACTCCAGTT GCGATACTAG CCTAAATTAC 
CATGATGTAA ATAATCTGCC AGCAGCGCAA GAGGATGAGA ATACAGTGAA ACCGGGCAAG 
GAAGAACTGT TGGAGGAGAG CACAAATAAA CCCAGCAACC AGGAGTCACA ACACCATCGT 
CTGGCCATCG ATGATATGCG CACCCGCCTG AATCAGTGCC GCAGCAAATT CGAGCATTTG 
GAGGAGGCTA GTCGGCGGAA CTTCAACCAG TCGCAGCACT CCATGAAGAA CTTTTTCAAA 
ACCCCACCGG TGGCCCTTAA GATGTTTCAG CGCCTGGAGC ACGAGGCTGC AGCCCTGAAT 
GGTGGCAACA ATTGCCCACC GCCACCACCA CGTACGCAGC GCATCAATCA GACGCCCATT 
TTCGGTCGAA AGAATCCGCC AGTGGCAATC GTCGGGCAAA AACTACAGCA TGCTGAGTCC 
CTAGAGGACC TGGCCTCCAG CGGAGTTGCC AAACAGCTGG CCACACCAGC ACCCAAACGC 
AGCAAAGCCA CAGCAACGAC CAAAGGCGGC CAGAGCAGCA ATCCGCCACT TTTCCTGCCG 
CCTAGCCTTA ACATAAGTGT CGCATTAAAT AGCAATGGAA ATGTGACCAC AACGACCAAT 
ACCAATAGTA GTTGTCCACC ATCCGCATCA GACTGGCTGC CGAAGAAGCA CAAGCTAACT 
TTGCCACTTC CGTCGGCCCA ACAGCAGCGA ACGAGCAGCA ATCATCGCCT GCCAATGTGC 
AATAACAAAG GGAAGACACT GAAGCCACTG CCAAGTCGCA CGGGCAGTCA AGGCATTCCC 
GCAAGCAATT GCGTCTCGCC GACGAGGAGC AGCAGGCCCG GATCACCCAC CAAGCATCTG 
GCCCAGAGGC ACACAGCCGC CACGGCGCAG CGACTGACAA ATGCGGCCGC CACTCACCAG 
CAGCAGCATC AGCAACAATC CTCGAAGACG ACGCGCCTGA ATATCCTGAG TCCGGAAAAG 
GTGCATCGCC TGGGCGCTCG ACTCACCGAT CAGAAGCAGA AAATGCGCGA GGAGGCCGCA 
GCGACGGCCT CGTCCGTCGG AGGAGCCGGT TGTGCGGCAG GAACAGCGGC TGGAAGCTTA 
AATGGTCATC GGACCATCGG ATCCTCCGGC ACACCGAATT CGGCGGTCGG TGAACGGAGG 
AGGCGAGCAG CTCCATCGCC GCCAGTTCGC ACGCATTTCA ATACGCGCTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511139.2
CDS	300..4073
Translation	MHHQQRLRANGGRGGTGLGAGSGPVSGGHSPLCAHCRGQLLPHPEEPIVMALGQQWHCDCFRCSVCEGHLHNWYFEREGLLYCREDYYGRFGDACQQCMAVITGPVMVAGEHKFHPECFCCTACGSFIGEGESYALVERSKLYCGQCYGKRSCQPADAKARITTAGKPMHSIRLVEIPKDATPGLRVDGVALDDGCPTVRITDLFCNFFLWLTSMAEQCCGAPYRIDVNLTNLHIGDRILEVNGTPVSDSSVEQIDKLIRSNEKMLQLTVEHDPVQVCRSCSQADIQRAMSASTLILPLSTSASSVEVGRERLYKTPGEQGTKARKLRQATNASTTIPPAAGATAMTQLKEKERCSSLSKLLDEQHQAQQHSAHPQLYDLSRTQSCRVVQKPQRIFRATDLVIGEKLGEGFFGKVFKVTHRQSGEVMVLKELHRADEEAQRNFIKEVAVLRLLDHRHVLKFIGVLYKDKKLHMVTEYVAGGCLKELIHDPAQVLPWPQRVRLARDIACGMSYLHSMNIIHRDLNSMNCLVREDRSVIVADFGLARSVDAPRLPSGNMTPGGYGSGANSDAPMSPSGTLRRSKSRQRRQRYTVVGNPYWMAPEMMKGLKYDEKVDVFSFGIMLCEIIGRVEADPDFMPRNSDFSLNQQEFREKFCAQCPEPFVKVAFVCCDLNPDMRPCFETLHVWLQRLADDLAADRVPPERLLHEIETFQEWYASSEDALSPTSQRSLNNLDELVKSAVDSEISPVEKEKENMVIKPQDIPKSPHLGKDFSPSGERLRDSMRARRRQRFLGAQEERRNLTPDTESKERALKKALKKCRPFGERGYLVDLRAGAELQLEDVRDLNTYSDVDSSCDTSLNYHDVNNLPAAQEDENTVKPGKEELLEESTNKPSNQESQHHRLAIDDMRTRLNQCRSKFEHLEEASRRNFNQSQHSMKNFFKTPPVALKMFQRLEHEAAALNGGNNCPPPPPRTQRINQTPIFGRKNPPVAIVGQKLQHAESLEDLASSGVAKQLATPAPKRSKATATTKGGQSSNPPLFLPPSLNISVALNSNGNVTTTTNTNSSCPPSASDWLPKKHKLTLPLPSAQQQRTSSNHRLPMCNNKGKTLKPLPSRTGSQGIPASNCVSPTRSSRPGSPTKHLAQRHTAATAQRLTNAAATHQQQHQQQSSKTTRLNILSPEKVHRLGARLTDQKQKMREEAAATASSVGGAGCAAGTAAGSLNGHRTIGSSGTPNSAVGERRRRAAPSPPVRTHFNTRC

Target ORF information:

RefSeq Version	NM_078584.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078584.3

ORF Insert Sequence:

ATGCATCATC AGCAGCGCCT GCGGGCAAAT GGCGGACGAG GAGGCACTGG ATTGGGCGCA 
GGATCAGGAC CTGTGTCCGG AGGACACTCA CCATTGTGCG CCCATTGTCG GGGTCAACTA 
CTGCCACATC CAGAGGAGCC AATCGTGATG GCTCTCGGCC AGCAATGGCA CTGCGATTGC 
TTTCGCTGCT CCGTTTGCGA GGGCCATCTG CATAACTGGT ACTTCGAACG GGAGGGCTTA 
CTGTACTGTC GTGAGGATTA CTACGGCCGA TTCGGCGATG CCTGTCAGCA GTGTATGGCC 
GTTATCACCG GACCCGTAAT GGTCGCCGGC GAGCACAAAT TCCATCCGGA GTGCTTCTGC 
TGCACGGCAT GCGGCTCCTT CATCGGCGAG GGCGAATCGT ACGCCCTTGT CGAGCGTTCG 
AAGCTCTATT GTGGCCAGTG CTATGGCAAA CGTTCCTGCC AGCCGGCGGA TGCGAAGGCG 
CGGATCACAA CGGCCGGCAA GCCGATGCAC TCCATCCGTC TGGTCGAGAT ACCCAAGGAC 
GCCACGCCCG GCCTGAGGGT CGACGGAGTG GCCCTCGACG ATGGTTGTCC CACGGTCCGC 
ATTACAGACT TGTTCTGTAA CTTCTTCTTG TGGCTAACCT CGATGGCGGA ACAGTGCTGT 
GGGGCGCCTT ATAGAATTGA TGTGAATCTT ACAAATCTGC ATATTGGAGA TAGGATATTG 
GAGGTGAACG GCACACCAGT TAGTGATTCG TCCGTGGAAC AGATCGACAA ATTGATACGG 
AGTAATGAGA AGATGCTTCA GCTGACCGTG GAGCATGATC CGGTGCAAGT GTGCCGCTCC 
TGCAGCCAAG CGGACATCCA GCGGGCCATG TCAGCGTCCA CGTTAATCCT GCCGCTCAGC 
ACATCGGCCT CCAGTGTCGA GGTGGGCAGG GAGCGTCTGT ACAAGACACC CGGCGAACAG 
GGCACGAAGG CCAGGAAACT GCGACAAGCC ACCAATGCCT CTACGACAAT ACCACCGGCA 
GCTGGAGCCA CCGCAATGAC GCAACTAAAG GAGAAGGAGC GTTGTTCCAG TCTGTCCAAG 
TTGCTGGATG AACAGCATCA GGCACAACAG CATTCGGCGC ATCCTCAGCT CTATGATCTG 
TCCAGGACGC AATCCTGCCG TGTCGTCCAA AAGCCACAAC GAATCTTTCG TGCAACTGAT 
CTGGTTATAG GAGAGAAGCT AGGCGAGGGC TTCTTTGGCA AGGTTTTCAA GGTGACCCAC 
CGTCAGAGCG GCGAGGTGAT GGTCCTCAAA GAACTGCATC GTGCGGATGA GGAAGCGCAA 
AGGAATTTCA TCAAGGAAGT GGCCGTTCTC CGTTTGCTGG ACCATCGGCA TGTGCTCAAA 
TTCATTGGTG TTCTGTACAA GGATAAGAAA CTGCACATGG TCACGGAGTA CGTGGCCGGT 
GGTTGTCTCA AGGAGCTGAT CCACGATCCA GCCCAAGTAC TCCCTTGGCC CCAGCGCGTC 
CGCTTGGCCA GGGATATTGC GTGCGGCATG AGCTATCTGC ACTCGATGAA CATCATCCAT 
CGCGATTTGA ACTCAATGAA TTGCCTGGTC CGTGAAGATC GCTCGGTCAT AGTGGCGGAT 
TTTGGACTAG CTCGCAGTGT GGATGCGCCG CGGCTGCCAA GTGGCAATAT GACGCCAGGC 
GGCTATGGAT CAGGCGCTAA CAGCGATGCG CCCATGTCAC CGAGTGGAAC ACTAAGGCGC 
AGCAAGAGCA GGCAGCGAAG GCAACGTTAC ACGGTGGTCG GGAATCCCTA CTGGATGGCA 
CCCGAGATGA TGAAAGGTCT CAAATACGAC GAAAAGGTGG ATGTCTTCTC CTTTGGCATT 
ATGTTGTGTG AGATCATTGG ACGCGTGGAA GCCGATCCGG ATTTTATGCC TCGCAATTCG 
GACTTCAGTC TCAATCAGCA GGAGTTTCGC GAGAAGTTCT GCGCTCAGTG TCCAGAGCCT 
TTCGTTAAAG TGGCCTTCGT GTGCTGTGAT CTTAATCCCG ATATGCGTCC GTGTTTCGAA 
ACTCTGCACG TTTGGCTGCA GCGTCTCGCC GATGATCTGG CCGCCGATCG TGTGCCGCCA 
GAGCGTCTGC TGCACGAGAT TGAGACCTTT CAGGAATGGT ACGCCAGCTC TGAGGACGCC 
TTGTCACCCA CCTCCCAGCG CAGCTTAAAT AATCTCGATG AGTTGGTCAA GAGTGCGGTG 
GATTCGGAAA TTTCGCCGGT AGAAAAAGAG AAGGAGAATA TGGTGATTAA GCCCCAGGAT 
ATACCGAAAT CTCCGCATCT CGGCAAGGAT TTCTCGCCCA GCGGCGAGAG ACTGCGTGAC 
AGTATGCGGG CCAGGCGTCG CCAACGTTTC TTGGGCGCCC AGGAGGAGCG TCGTAATCTC 
ACGCCCGACA CGGAGTCCAA GGAAAGGGCT TTGAAGAAGG CGCTGAAGAA ATGTCGTCCG 
TTTGGCGAAA GAGGTTACCT GGTGGATCTG CGTGCCGGTG CCGAATTGCA GCTAGAGGAT 
GTCAGGGATT TGAACACCTA TTCCGATGTG GACTCCAGTT GCGATACTAG CCTAAATTAC 
CATGATGTAA ATAATCTGCC AGCAGCGCAA GAGGATGAGA ATACAGTGAA ACCGGGCAAG 
GAAGAACTGT TGGAGGAGAG CACAAATAAA CCCAGCAACC AGGAGTCACA ACACCATCGT 
CTGGCCATCG ATGATATGCG CACCCGCCTG AATCAGTGCC GCAGCAAATT CGAGCATTTG 
GAGGAGGCTA GTCGGCGGAA CTTCAACCAG TCGCAGCACT CCATGAAGAA CTTTTTCAAA 
ACCCCACCGG TGGCCCTTAA GATGTTTCAG CGCCTGGAGC ACGAGGCTGC AGCCCTGAAT 
GGTGGCAACA ATTGCCCACC GCCACCACCA CGTACGCAGC GCATCAATCA GACGCCCATT 
TTCGGTCGAA AGAATCCGCC AGTGGCAATC GTCGGGCAAA AACTACAGCA TGCTGAGTCC 
CTAGAGGACC TGGCCTCCAG CGGAGTTGCC AAACAGCTGG CCACACCAGC ACCCAAACGC 
AGCAAAGCCA CAGCAACGAC CAAAGGCGGC CAGAGCAGCA ATCCGCCACT TTTCCTGCCG 
CCTAGCCTTA ACATAAGTGT CGCATTAAAT AGCAATGGAA ATGTGACCAC AACGACCAAT 
ACCAATAGTA GTTGTCCACC ATCCGCATCA GACTGGCTGC CGAAGAAGCA CAAGCTAACT 
TTGCCACTTC CGTCGGCCCA ACAGCAGCGA ACGAGCAGCA ATCATCGCCT GCCAATGTGC 
AATAACAAAG GGAAGACACT GAAGCCACTG CCAAGTCGCA CGGGCAGTCA AGGCATTCCC 
GCAAGCAATT GCGTCTCGCC GACGAGGAGC AGCAGGCCCG GATCACCCAC CAAGCATCTG 
GCCCAGAGGC ACACAGCCGC CACGGCGCAG CGACTGACAA ATGCGGCCGC CACTCACCAG 
CAGCAGCATC AGCAACAATC CTCGAAGACG ACGCGCCTGA ATATCCTGAG TCCGGAAAAG 
GTGCATCGCC TGGGCGCTCG ACTCACCGAT CAGAAGCAGA AAATGCGCGA GGAGGCCGCA 
GCGACGGCCT CGTCCGTCGG AGGAGCCGGT TGTGCGGCAG GAACAGCGGC TGGAAGCTTA 
AATGGTCATC GGACCATCGG ATCCTCCGGC ACACCGAATT CGGCGGTCGG TGAACGGAGG 
AGGCGAGCAG CTCCATCGCC GCCAGTTCGC ACGCATTTCA ATACGCGCTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMK1 ( NM_167327.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727622.1 Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa01406
Clone ID Related Accession (Same CDS sequence)	NM_167327.2
Accession Version	NM_167327.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	LIM domain kinase 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_167327.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTTTT GGTTACATTT TGTACTGAAA AAAATTTTAC CATGGCTCAA ATCTACGAAA 
ATTGATGTGA ATCTTACAAA TCTGCATATT GGAGATAGGA TATTGGAGGT GAACGGCACA 
CCAGTTAGTG ATTCGTCCGT GGAACAGATC GACAAATTGA TACGGAGTAA TGAGAAGATG 
CTTCAGCTGA CCGTGGAGCA TGATCCGGTG CAAGTGTGCC GCTCCTGCAG CCAAGCGGAC 
ATCCAGCGGG CCATGTCAGC GTCCACGTTA ATCCTGCCGC TCAGCACATC GGCCTCCAGT 
GTCGAGGTGG GCAGGGAGCG TCTGTACAAG ACACCCGGCG AACAGGGCAC GAAGGCCAGG 
AAACTGCGAC AAGCCACCAA TGCCTCTACG ACAATACCAC CGGCAGCTGG AGCCACCGCA 
ATGACGCAAC TAAAGGAGAA GGAGCGTTGT TCCAGTCTGT CCAAGTTGCT GGATGAACAG 
CATCAGGCAC AACAGCATTC GGCGCATCCT CAGCTCTATG ATCTGTCCAG GACGCAATCC 
TGCCGTGTCG TCCAAAAGCC ACAACGAATC TTTCGTGCAA CTGATCTGGT TATAGGAGAG 
AAGCTAGGCG AGGGCTTCTT TGGCAAGGTT TTCAAGGTGA CCCACCGTCA GAGCGGCGAG 
GTGATGGTCC TCAAAGAACT GCATCGTGCG GATGAGGAAG CGCAAAGGAA TTTCATCAAG 
GAAGTGGCCG TTCTCCGTTT GCTGGACCAT CGGCATGTGC TCAAATTCAT TGGTGTTCTG 
TACAAGGATA AGAAACTGCA CATGGTCACG GAGTACGTGG CCGGTGGTTG TCTCAAGGAG 
CTGATCCACG ATCCAGCCCA AGTACTCCCT TGGCCCCAGC GCGTCCGCTT GGCCAGGGAT 
ATTGCGTGCG GCATGAGCTA TCTGCACTCG ATGAACATCA TCCATCGCGA TTTGAACTCA 
ATGAATTGCC TGGTCCGTGA AGATCGCTCG GTCATAGTGG CGGATTTTGG ACTAGCTCGC 
AGTGTGGATG CGCCGCGGCT GCCAAGTGGC AATATGACGC CAGGCGGCTA TGGATCAGGC 
GCTAACAGCG ATGCGCCCAT GTCACCGAGT GGAACACTAA GGCGCAGCAA GAGCAGGCAG 
CGAAGGCAAC GTTACACGGT GGTCGGGAAT CCCTACTGGA TGGCACCCGA GATGATGAAA 
GGTCTCAAAT ACGACGAAAA GGTGGATGTC TTCTCCTTTG GCATTATGTT GTGTGAGATC 
ATTGGACGCG TGGAAGCCGA TCCGGATTTT ATGCCTCGCA ATTCGGACTT CAGTCTCAAT 
CAGCAGGAGT TTCGCGAGAA GTTCTGCGCT CAGTGTCCAG AGCCTTTCGT TAAAGTGGCC 
TTCGTGTGCT GTGATCTTAA TCCCGATATG CGTCCGTGTT TCGAAACTCT GCACGTTTGG 
CTGCAGCGTC TCGCCGATGA TCTGGCCGCC GATCGTGTGC CGCCAGAGCG TCTGCTGCAC 
GAGATTGAGA CCTTTCAGGA ATGGTACGCC AGCTCTGAGG ACGCCTTGTC ACCCACCTCC 
CAGCGCAGCT TAAATAATCT CGATGAGTTG GTCAAGAGTG CGGTGGATTC GGAAATTTCG 
CCGGTAGAAA AAGAGAAGGA GAATATGGTG ATTAAGCCCC AGGATATACC GAAATCTCCG 
CATCTCGGCA AGGATTTCTC GCCCAGCGGC GAGAGACTGC GTGACAGTAT GCGGGCCAGG 
CGTCGCCAAC GTTTCTTGGG CGCCCAGGAG GAGCGTCGTA ATCTCACGCC CGACACGGAG 
TCCAAGGAAA GGGCTTTGAA GAAGGCGCTG AAGAAATGTC GTCCGTTTGG CGAAAGAGGT 
TACCTGGTGG ATCTGCGTGC CGGTGCCGAA TTGCAGCTAG AGGATGTCAG GGATTTGAAC 
ACCTATTCCG ATGTGGACTC CAGTTGCGAT ACTAGCCTAA ATTACCATGA TGTAAATAAT 
CTGCCAGCAG CGCAAGAGGA TGAGAATACA GTGAAACCGG GCAAGGAAGA ACTGTTGGAG 
GAGAGCACAA ATAAACCCAG CAACCAGGAG TCACAACACC ATCGTCTGGC CATCGATGAT 
ATGCGCACCC GCCTGAATCA GTGCCGCAGC AAATTCGAGC ATTTGGAGGA GGCTAGTCGG 
CGGAACTTCA ACCAGTCGCA GCACTCCATG AAGAACTTTT TCAAAACCCC ACCGGTGGCC 
CTTAAGATGT TTCAGCGCCT GGAGCACGAG GCTGCAGCCC TGAATGGTGG CAACAATTGC 
CCACCGCCAC CACCACGTAC GCAGCGCATC AATCAGACGC CCATTTTCGG TCGAAAGAAT 
CCGCCAGTGG CAATCGTCGG GCAAAAACTA CAGCATGCTG AGTCCCTAGA GGACCTGGCC 
TCCAGCGGAG TTGCCAAACA GCTGGCCACA CCAGCACCCA AACGCAGCAA AGCCACAGCA 
ACGACCAAAG GCGGCCAGAG CAGCAATCCG CCACTTTTCC TGCCGCCTAG CCTTAACATA 
AGTGTCGCAT TAAATAGCAA TGGAAATGTG ACCACAACGA CCAATACCAA TAGTAGTTGT 
CCACCATCCG CATCAGACTG GCTGCCGAAG AAGCACAAGC TAACTTTGCC ACTTCCGTCG 
GCCCAACAGC AGCGAACGAG CAGCAATCAT CGCCTGCCAA TGTGCAATAA CAAAGGGAAG 
ACACTGAAGC CACTGCCAAG TCGCACGGGC AGTCAAGGCA TTCCCGCAAG CAATTGCGTC 
TCGCCGACGA GGAGCAGCAG GCCCGGATCA CCCACCAAGC ATCTGGCCCA GAGGCACACA 
GCCGCCACGG CGCAGCGACT GACAAATGCG GCCGCCACTC ACCAGCAGCA GCATCAGCAA 
CAATCCTCGA AGACGACGCG CCTGAATATC CTGAGTCCGG AAAAGGTGCA TCGCCTGGGC 
GCTCGACTCA CCGATCAGAA GCAGAAAATG CGCGAGGAGG CCGCAGCGAC GGCCTCGTCC 
GTCGGAGGAG CCGGTTGTGC GGCAGGAACA GCGGCTGGAA GCTTAAATGG TCATCGGACC 
ATCGGATCCT CCGGCACACC GAATTCGGCG GTCGGTGAAC GGAGGAGGCG AGCAGCTCCA 
TCGCCGCCAG TTCGCACGCA TTTCAATACG CGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727622.1
CDS	102..3260
Translation	MSFWLHFVLKKILPWLKSTKIDVNLTNLHIGDRILEVNGTPVSDSSVEQIDKLIRSNEKMLQLTVEHDPVQVCRSCSQADIQRAMSASTLILPLSTSASSVEVGRERLYKTPGEQGTKARKLRQATNASTTIPPAAGATAMTQLKEKERCSSLSKLLDEQHQAQQHSAHPQLYDLSRTQSCRVVQKPQRIFRATDLVIGEKLGEGFFGKVFKVTHRQSGEVMVLKELHRADEEAQRNFIKEVAVLRLLDHRHVLKFIGVLYKDKKLHMVTEYVAGGCLKELIHDPAQVLPWPQRVRLARDIACGMSYLHSMNIIHRDLNSMNCLVREDRSVIVADFGLARSVDAPRLPSGNMTPGGYGSGANSDAPMSPSGTLRRSKSRQRRQRYTVVGNPYWMAPEMMKGLKYDEKVDVFSFGIMLCEIIGRVEADPDFMPRNSDFSLNQQEFREKFCAQCPEPFVKVAFVCCDLNPDMRPCFETLHVWLQRLADDLAADRVPPERLLHEIETFQEWYASSEDALSPTSQRSLNNLDELVKSAVDSEISPVEKEKENMVIKPQDIPKSPHLGKDFSPSGERLRDSMRARRRQRFLGAQEERRNLTPDTESKERALKKALKKCRPFGERGYLVDLRAGAELQLEDVRDLNTYSDVDSSCDTSLNYHDVNNLPAAQEDENTVKPGKEELLEESTNKPSNQESQHHRLAIDDMRTRLNQCRSKFEHLEEASRRNFNQSQHSMKNFFKTPPVALKMFQRLEHEAAALNGGNNCPPPPPRTQRINQTPIFGRKNPPVAIVGQKLQHAESLEDLASSGVAKQLATPAPKRSKATATTKGGQSSNPPLFLPPSLNISVALNSNGNVTTTTNTNSSCPPSASDWLPKKHKLTLPLPSAQQQRTSSNHRLPMCNNKGKTLKPLPSRTGSQGIPASNCVSPTRSSRPGSPTKHLAQRHTAATAQRLTNAAATHQQQHQQQSSKTTRLNILSPEKVHRLGARLTDQKQKMREEAAATASSVGGAGCAAGTAAGSLNGHRTIGSSGTPNSAVGERRRRAAPSPPVRTHFNTRC

Target ORF information:

RefSeq Version	NM_167327.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167327.2

ORF Insert Sequence:

ATGTCTTTTT GGTTACATTT TGTACTGAAA AAAATTTTAC CATGGCTCAA ATCTACGAAA 
ATTGATGTGA ATCTTACAAA TCTGCATATT GGAGATAGGA TATTGGAGGT GAACGGCACA 
CCAGTTAGTG ATTCGTCCGT GGAACAGATC GACAAATTGA TACGGAGTAA TGAGAAGATG 
CTTCAGCTGA CCGTGGAGCA TGATCCGGTG CAAGTGTGCC GCTCCTGCAG CCAAGCGGAC 
ATCCAGCGGG CCATGTCAGC GTCCACGTTA ATCCTGCCGC TCAGCACATC GGCCTCCAGT 
GTCGAGGTGG GCAGGGAGCG TCTGTACAAG ACACCCGGCG AACAGGGCAC GAAGGCCAGG 
AAACTGCGAC AAGCCACCAA TGCCTCTACG ACAATACCAC CGGCAGCTGG AGCCACCGCA 
ATGACGCAAC TAAAGGAGAA GGAGCGTTGT TCCAGTCTGT CCAAGTTGCT GGATGAACAG 
CATCAGGCAC AACAGCATTC GGCGCATCCT CAGCTCTATG ATCTGTCCAG GACGCAATCC 
TGCCGTGTCG TCCAAAAGCC ACAACGAATC TTTCGTGCAA CTGATCTGGT TATAGGAGAG 
AAGCTAGGCG AGGGCTTCTT TGGCAAGGTT TTCAAGGTGA CCCACCGTCA GAGCGGCGAG 
GTGATGGTCC TCAAAGAACT GCATCGTGCG GATGAGGAAG CGCAAAGGAA TTTCATCAAG 
GAAGTGGCCG TTCTCCGTTT GCTGGACCAT CGGCATGTGC TCAAATTCAT TGGTGTTCTG 
TACAAGGATA AGAAACTGCA CATGGTCACG GAGTACGTGG CCGGTGGTTG TCTCAAGGAG 
CTGATCCACG ATCCAGCCCA AGTACTCCCT TGGCCCCAGC GCGTCCGCTT GGCCAGGGAT 
ATTGCGTGCG GCATGAGCTA TCTGCACTCG ATGAACATCA TCCATCGCGA TTTGAACTCA 
ATGAATTGCC TGGTCCGTGA AGATCGCTCG GTCATAGTGG CGGATTTTGG ACTAGCTCGC 
AGTGTGGATG CGCCGCGGCT GCCAAGTGGC AATATGACGC CAGGCGGCTA TGGATCAGGC 
GCTAACAGCG ATGCGCCCAT GTCACCGAGT GGAACACTAA GGCGCAGCAA GAGCAGGCAG 
CGAAGGCAAC GTTACACGGT GGTCGGGAAT CCCTACTGGA TGGCACCCGA GATGATGAAA 
GGTCTCAAAT ACGACGAAAA GGTGGATGTC TTCTCCTTTG GCATTATGTT GTGTGAGATC 
ATTGGACGCG TGGAAGCCGA TCCGGATTTT ATGCCTCGCA ATTCGGACTT CAGTCTCAAT 
CAGCAGGAGT TTCGCGAGAA GTTCTGCGCT CAGTGTCCAG AGCCTTTCGT TAAAGTGGCC 
TTCGTGTGCT GTGATCTTAA TCCCGATATG CGTCCGTGTT TCGAAACTCT GCACGTTTGG 
CTGCAGCGTC TCGCCGATGA TCTGGCCGCC GATCGTGTGC CGCCAGAGCG TCTGCTGCAC 
GAGATTGAGA CCTTTCAGGA ATGGTACGCC AGCTCTGAGG ACGCCTTGTC ACCCACCTCC 
CAGCGCAGCT TAAATAATCT CGATGAGTTG GTCAAGAGTG CGGTGGATTC GGAAATTTCG 
CCGGTAGAAA AAGAGAAGGA GAATATGGTG ATTAAGCCCC AGGATATACC GAAATCTCCG 
CATCTCGGCA AGGATTTCTC GCCCAGCGGC GAGAGACTGC GTGACAGTAT GCGGGCCAGG 
CGTCGCCAAC GTTTCTTGGG CGCCCAGGAG GAGCGTCGTA ATCTCACGCC CGACACGGAG 
TCCAAGGAAA GGGCTTTGAA GAAGGCGCTG AAGAAATGTC GTCCGTTTGG CGAAAGAGGT 
TACCTGGTGG ATCTGCGTGC CGGTGCCGAA TTGCAGCTAG AGGATGTCAG GGATTTGAAC 
ACCTATTCCG ATGTGGACTC CAGTTGCGAT ACTAGCCTAA ATTACCATGA TGTAAATAAT 
CTGCCAGCAG CGCAAGAGGA TGAGAATACA GTGAAACCGG GCAAGGAAGA ACTGTTGGAG 
GAGAGCACAA ATAAACCCAG CAACCAGGAG TCACAACACC ATCGTCTGGC CATCGATGAT 
ATGCGCACCC GCCTGAATCA GTGCCGCAGC AAATTCGAGC ATTTGGAGGA GGCTAGTCGG 
CGGAACTTCA ACCAGTCGCA GCACTCCATG AAGAACTTTT TCAAAACCCC ACCGGTGGCC 
CTTAAGATGT TTCAGCGCCT GGAGCACGAG GCTGCAGCCC TGAATGGTGG CAACAATTGC 
CCACCGCCAC CACCACGTAC GCAGCGCATC AATCAGACGC CCATTTTCGG TCGAAAGAAT 
CCGCCAGTGG CAATCGTCGG GCAAAAACTA CAGCATGCTG AGTCCCTAGA GGACCTGGCC 
TCCAGCGGAG TTGCCAAACA GCTGGCCACA CCAGCACCCA AACGCAGCAA AGCCACAGCA 
ACGACCAAAG GCGGCCAGAG CAGCAATCCG CCACTTTTCC TGCCGCCTAG CCTTAACATA 
AGTGTCGCAT TAAATAGCAA TGGAAATGTG ACCACAACGA CCAATACCAA TAGTAGTTGT 
CCACCATCCG CATCAGACTG GCTGCCGAAG AAGCACAAGC TAACTTTGCC ACTTCCGTCG 
GCCCAACAGC AGCGAACGAG CAGCAATCAT CGCCTGCCAA TGTGCAATAA CAAAGGGAAG 
ACACTGAAGC CACTGCCAAG TCGCACGGGC AGTCAAGGCA TTCCCGCAAG CAATTGCGTC 
TCGCCGACGA GGAGCAGCAG GCCCGGATCA CCCACCAAGC ATCTGGCCCA GAGGCACACA 
GCCGCCACGG CGCAGCGACT GACAAATGCG GCCGCCACTC ACCAGCAGCA GCATCAGCAA 
CAATCCTCGA AGACGACGCG CCTGAATATC CTGAGTCCGG AAAAGGTGCA TCGCCTGGGC 
GCTCGACTCA CCGATCAGAA GCAGAAAATG CGCGAGGAGG CCGCAGCGAC GGCCTCGTCC 
GTCGGAGGAG CCGGTTGTGC GGCAGGAACA GCGGCTGGAA GCTTAAATGG TCATCGGACC 
ATCGGATCCT CCGGCACACC GAATTCGGCG GTCGGTGAAC GGAGGAGGCG AGCAGCTCCA 
TCGCCGCCAG TTCGCACGCA TTTCAATACG CGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMK1 ( NM_001169262.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162733.1 Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant E, mRNA.

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CloneID	OFa01407
Clone ID Related Accession (Same CDS sequence)	NM_001169262.1 , NM_167326.2
Accession Version	NM_001169262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	LIM domain kinase 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCATC AGCAGCGCCT GCGGGCAAAT GGCGGACGAG GAGGCACTGG ATTGGGCGCA 
GGATCAGGAC CTGTGTCCGG AGGACACTCA CCATTGTGCG CCCATTGTCG GGGTCAACTA 
CTGCCACATC CAGAGGAGCC AATCGTGATG GCTCTCGGCC AGCAATGGCA CTGCGATTGC 
TTTCGCTGCT CCGTTTGCGA GGGCCATCTG CATAACTGGT ACTTCGAACG GGAGGGCTTA 
CTGTACTGTC GTGAGGATTA CTACGGCCGA TTCGGCGATG CCTGTCAGCA GTGTATGGCC 
GTTATCACCG GACCCGTAAT GGTCGCCGGC GAGCACAAAT TCCATCCGGA GTGCTTCTGC 
TGCACGGCAT GCGGCTCCTT CATCGGCGAG GGCGAATCGT ACGCCCTTGT CGAGCGTTCG 
AAGCTCTATT GTGGCCAGTG CTATGGCAAA CGTTCCTGCC AGCCGGCGGA TGCGAAGGCG 
CGGATCACAA CGGCCGGCAA GCCGATGCAC TCCATCCGTC TGGTCGAGAT ACCCAAGGAC 
GCCACGCCCG GCCTGAGGGT CGACGGAGTG GCCCTCGACG ATGGTTGTCC CACGGTCCGC 
ATTACAGAAA TTGATGTGAA TCTTACAAAT CTGCATATTG GAGATAGGAT ATTGGAGGTG 
AACGGCACAC CAGTTAGTGA TTCGTCCGTG GAACAGATCG ACAAATTGAT ACGGAGTAAT 
GAGAAGATGC TTCAGCTGAC CGTGGAGCAT GATCCGGTGC AAGTGTGCCG CTCCTGCAGC 
CAAGCGGACA TCCAGCGGGC CATGTCAGCG TCCACGTTAA TCCTGCCGCT CAGCACATCG 
GCCTCCAGTG TCGAGGTGGG CAGGGAGCGT CTGTACAAGA CACCCGGCGA ACAGGGCACG 
AAGGCCAGGA AACTGCGACA AGCCACCAAT GCCTCTACGA CAATACCACC GGCAGCTGGA 
GCCACCGCAA TGACGCAACT AAAGGAGAAG GAGCGTTGTT CCAGTCTGTC CAAGTTGCTG 
GATGAACAGC ATCAGGCACA ACAGCATTCG GCGCATCCTC AGCTCTATGA TCTGTCCAGG 
ACGCAATCCT GCCGTGTCGT CCAAAAGCCA CAACGAATCT TTCGTGCAAC TGATCTGGTT 
ATAGGAGAGA AGCTAGGCGA GGGCTTCTTT GGCAAGGTTT TCAAGGTGAC CCACCGTCAG 
AGCGGCGAGG TGATGGTCCT CAAAGAACTG CATCGTGCGG ATGAGGAAGC GCAAAGGAAT 
TTCATCAAGG AAGTGGCCGT TCTCCGTTTG CTGGACCATC GGCATGTGCT CAAATTCATT 
GGTGTTCTGT ACAAGGATAA GAAACTGCAC ATGGTCACGG AGTACGTGGC CGGTGGTTGT 
CTCAAGGAGC TGATCCACGA TCCAGCCCAA GTACTCCCTT GGCCCCAGCG CGTCCGCTTG 
GCCAGGGATA TTGCGTGCGG CATGAGCTAT CTGCACTCGA TGAACATCAT CCATCGCGAT 
TTGAACTCAA TGAATTGCCT GGTCCGTGAA GATCGCTCGG TCATAGTGGC GGATTTTGGA 
CTAGCTCGCA GTGTGGATGC GCCGCGGCTG CCAAGTGGCA ATATGACGCC AGGCGGCTAT 
GGATCAGGCG CTAACAGCGA TGCGCCCATG TCACCGAGTG GAACACTAAG GCGCAGCAAG 
AGCAGGCAGC GAAGGCAACG TTACACGGTG GTCGGGAATC CCTACTGGAT GGCACCCGAG 
ATGATGAAAG GTCTCAAATA CGACGAAAAG GTGGATGTCT TCTCCTTTGG CATTATGTTG 
TGTGAGATCA TTGGACGCGT GGAAGCCGAT CCGGATTTTA TGCCTCGCAA TTCGGACTTC 
AGTCTCAATC AGCAGGAGTT TCGCGAGAAG TTCTGCGCTC AGTGTCCAGA GCCTTTCGTT 
AAAGTGGCCT TCGTGTGCTG TGATCTTAAT CCCGATATGC GTCCGTGTTT CGAAACTCTG 
CACGTTTGGC TGCAGCGTCT CGCCGATGAT CTGGCCGCCG ATCGTGTGCC GCCAGAGCGT 
CTGCTGCACG AGATTGAGAC CTTTCAGGAA TGGTACGCCA GCTCTGAGGA CGCCTTGTCA 
CCCACCTCCC AGCGCAGCTT AAATAATCTC GATGAGTTGG TCAAGAGTGC GGTGGATTCG 
GAAATTTCGC CGGTAGAAAA AGAGAAGGAG AATATGGTGA TTAAGCCCCA GGATATACCG 
AAATCTCCGC ATCTCGGCAA GGATTTCTCG CCCAGCGGCG AGAGACTGCG TGACAGTATG 
CGGGCCAGGC GTCGCCAACG TTTCTTGGGC GCCCAGGAGG AGCGTCGTAA TCTCACGCCC 
GACACGGAGT CCAAGGAAAG GGCTTTGAAG AAGGCGCTGA AGAAATGTCG TCCGTTTGGC 
GAAAGAGGTT ACCTGGTGGA TCTGCGTGCC GGTGCCGAAT TGCAGCTAGA GGATGTCAGG 
GATTTGAACA CCTATTCCGA TGTGGACTCC AGTTGCGATA CTAGCCTAAA TTACCATGAT 
GTAAATAATC TGCCAGCAGC GCAAGAGGAT GAGAATACAG TGAAACCGGG CAAGGAAGAA 
CTGTTGGAGG AGAGCACAAA TAAACCCAGC AACCAGGAGT CACAACACCA TCGTCTGGCC 
ATCGATGATA TGCGCACCCG CCTGAATCAG TGCCGCAGCA AATTCGAGCA TTTGGAGGAG 
GCTAGTCGGC GGAACTTCAA CCAGTCGCAG CACTCCATGA AGAACTTTTT CAAAACCCCA 
CCGGTGGCCC TTAAGATGTT TCAGCGCCTG GAGCACGAGG CTGCAGCCCT GAATGGTGGC 
AACAATTGCC CACCGCCACC ACCACGTACG CAGCGCATCA ATCAGACGCC CATTTTCGGT 
CGAAAGAATC CGCCAGTGGC AATCGTCGGG CAAAAACTAC AGCATGCTGA GTCCCTAGAG 
GACCTGGCCT CCAGCGGAGT TGCCAAACAG CTGGCCACAC CAGCACCCAA ACGCAGCAAA 
GCCACAGCAA CGACCAAAGG CGGCCAGAGC AGCAATCCGC CACTTTTCCT GCCGCCTAGC 
CTTAACATAA GTGTCGCATT AAATAGCAAT GGAAATGTGA CCACAACGAC CAATACCAAT 
AGTAGTTGTC CACCATCCGC ATCAGACTGG CTGCCGAAGA AGCACAAGCT AACTTTGCCA 
CTTCCGTCGG CCCAACAGCA GCGAACGAGC AGCAATCATC GCCTGCCAAT GTGCAATAAC 
AAAGGGAAGA CACTGAAGCC ACTGCCAAGT CGCACGGGCA GTCAAGGCAT TCCCGCAAGC 
AATTGCGTCT CGCCGACGAG GAGCAGCAGG CCCGGATCAC CCACCAAGCA TCTGGCCCAG 
AGGCACACAG CCGCCACGGC GCAGCGACTG ACAAATGCGG CCGCCACTCA CCAGCAGCAG 
CATCAGCAAC AATCCTCGAA GACGACGCGC CTGAATATCC TGAGTCCGGA AAAGGTGCAT 
CGCCTGGGCG CTCGACTCAC CGATCAGAAG CAGAAAATGC GCGAGGAGGC CGCAGCGACG 
GCCTCGTCCG TCGGAGGAGC CGGTTGTGCG GCAGGAACAG CGGCTGGAAG CTTAAATGGT 
CATCGGACCA TCGGATCCTC CGGCACACCG AATTCGGCGG TCGGTGAACG GAGGAGGCGA 
GCAGCTCCAT CGCCGCCAGT TCGCACGCAT TTCAATACGC GCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162733.1
CDS	300..4007
Translation	MHHQQRLRANGGRGGTGLGAGSGPVSGGHSPLCAHCRGQLLPHPEEPIVMALGQQWHCDCFRCSVCEGHLHNWYFEREGLLYCREDYYGRFGDACQQCMAVITGPVMVAGEHKFHPECFCCTACGSFIGEGESYALVERSKLYCGQCYGKRSCQPADAKARITTAGKPMHSIRLVEIPKDATPGLRVDGVALDDGCPTVRITEIDVNLTNLHIGDRILEVNGTPVSDSSVEQIDKLIRSNEKMLQLTVEHDPVQVCRSCSQADIQRAMSASTLILPLSTSASSVEVGRERLYKTPGEQGTKARKLRQATNASTTIPPAAGATAMTQLKEKERCSSLSKLLDEQHQAQQHSAHPQLYDLSRTQSCRVVQKPQRIFRATDLVIGEKLGEGFFGKVFKVTHRQSGEVMVLKELHRADEEAQRNFIKEVAVLRLLDHRHVLKFIGVLYKDKKLHMVTEYVAGGCLKELIHDPAQVLPWPQRVRLARDIACGMSYLHSMNIIHRDLNSMNCLVREDRSVIVADFGLARSVDAPRLPSGNMTPGGYGSGANSDAPMSPSGTLRRSKSRQRRQRYTVVGNPYWMAPEMMKGLKYDEKVDVFSFGIMLCEIIGRVEADPDFMPRNSDFSLNQQEFREKFCAQCPEPFVKVAFVCCDLNPDMRPCFETLHVWLQRLADDLAADRVPPERLLHEIETFQEWYASSEDALSPTSQRSLNNLDELVKSAVDSEISPVEKEKENMVIKPQDIPKSPHLGKDFSPSGERLRDSMRARRRQRFLGAQEERRNLTPDTESKERALKKALKKCRPFGERGYLVDLRAGAELQLEDVRDLNTYSDVDSSCDTSLNYHDVNNLPAAQEDENTVKPGKEELLEESTNKPSNQESQHHRLAIDDMRTRLNQCRSKFEHLEEASRRNFNQSQHSMKNFFKTPPVALKMFQRLEHEAAALNGGNNCPPPPPRTQRINQTPIFGRKNPPVAIVGQKLQHAESLEDLASSGVAKQLATPAPKRSKATATTKGGQSSNPPLFLPPSLNISVALNSNGNVTTTTNTNSSCPPSASDWLPKKHKLTLPLPSAQQQRTSSNHRLPMCNNKGKTLKPLPSRTGSQGIPASNCVSPTRSSRPGSPTKHLAQRHTAATAQRLTNAAATHQQQHQQQSSKTTRLNILSPEKVHRLGARLTDQKQKMREEAAATASSVGGAGCAAGTAAGSLNGHRTIGSSGTPNSAVGERRRRAAPSPPVRTHFNTRC

Target ORF information:

RefSeq Version	NM_001169262.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169262.1

ORF Insert Sequence:

ATGCATCATC AGCAGCGCCT GCGGGCAAAT GGCGGACGAG GAGGCACTGG ATTGGGCGCA 
GGATCAGGAC CTGTGTCCGG AGGACACTCA CCATTGTGCG CCCATTGTCG GGGTCAACTA 
CTGCCACATC CAGAGGAGCC AATCGTGATG GCTCTCGGCC AGCAATGGCA CTGCGATTGC 
TTTCGCTGCT CCGTTTGCGA GGGCCATCTG CATAACTGGT ACTTCGAACG GGAGGGCTTA 
CTGTACTGTC GTGAGGATTA CTACGGCCGA TTCGGCGATG CCTGTCAGCA GTGTATGGCC 
GTTATCACCG GACCCGTAAT GGTCGCCGGC GAGCACAAAT TCCATCCGGA GTGCTTCTGC 
TGCACGGCAT GCGGCTCCTT CATCGGCGAG GGCGAATCGT ACGCCCTTGT CGAGCGTTCG 
AAGCTCTATT GTGGCCAGTG CTATGGCAAA CGTTCCTGCC AGCCGGCGGA TGCGAAGGCG 
CGGATCACAA CGGCCGGCAA GCCGATGCAC TCCATCCGTC TGGTCGAGAT ACCCAAGGAC 
GCCACGCCCG GCCTGAGGGT CGACGGAGTG GCCCTCGACG ATGGTTGTCC CACGGTCCGC 
ATTACAGAAA TTGATGTGAA TCTTACAAAT CTGCATATTG GAGATAGGAT ATTGGAGGTG 
AACGGCACAC CAGTTAGTGA TTCGTCCGTG GAACAGATCG ACAAATTGAT ACGGAGTAAT 
GAGAAGATGC TTCAGCTGAC CGTGGAGCAT GATCCGGTGC AAGTGTGCCG CTCCTGCAGC 
CAAGCGGACA TCCAGCGGGC CATGTCAGCG TCCACGTTAA TCCTGCCGCT CAGCACATCG 
GCCTCCAGTG TCGAGGTGGG CAGGGAGCGT CTGTACAAGA CACCCGGCGA ACAGGGCACG 
AAGGCCAGGA AACTGCGACA AGCCACCAAT GCCTCTACGA CAATACCACC GGCAGCTGGA 
GCCACCGCAA TGACGCAACT AAAGGAGAAG GAGCGTTGTT CCAGTCTGTC CAAGTTGCTG 
GATGAACAGC ATCAGGCACA ACAGCATTCG GCGCATCCTC AGCTCTATGA TCTGTCCAGG 
ACGCAATCCT GCCGTGTCGT CCAAAAGCCA CAACGAATCT TTCGTGCAAC TGATCTGGTT 
ATAGGAGAGA AGCTAGGCGA GGGCTTCTTT GGCAAGGTTT TCAAGGTGAC CCACCGTCAG 
AGCGGCGAGG TGATGGTCCT CAAAGAACTG CATCGTGCGG ATGAGGAAGC GCAAAGGAAT 
TTCATCAAGG AAGTGGCCGT TCTCCGTTTG CTGGACCATC GGCATGTGCT CAAATTCATT 
GGTGTTCTGT ACAAGGATAA GAAACTGCAC ATGGTCACGG AGTACGTGGC CGGTGGTTGT 
CTCAAGGAGC TGATCCACGA TCCAGCCCAA GTACTCCCTT GGCCCCAGCG CGTCCGCTTG 
GCCAGGGATA TTGCGTGCGG CATGAGCTAT CTGCACTCGA TGAACATCAT CCATCGCGAT 
TTGAACTCAA TGAATTGCCT GGTCCGTGAA GATCGCTCGG TCATAGTGGC GGATTTTGGA 
CTAGCTCGCA GTGTGGATGC GCCGCGGCTG CCAAGTGGCA ATATGACGCC AGGCGGCTAT 
GGATCAGGCG CTAACAGCGA TGCGCCCATG TCACCGAGTG GAACACTAAG GCGCAGCAAG 
AGCAGGCAGC GAAGGCAACG TTACACGGTG GTCGGGAATC CCTACTGGAT GGCACCCGAG 
ATGATGAAAG GTCTCAAATA CGACGAAAAG GTGGATGTCT TCTCCTTTGG CATTATGTTG 
TGTGAGATCA TTGGACGCGT GGAAGCCGAT CCGGATTTTA TGCCTCGCAA TTCGGACTTC 
AGTCTCAATC AGCAGGAGTT TCGCGAGAAG TTCTGCGCTC AGTGTCCAGA GCCTTTCGTT 
AAAGTGGCCT TCGTGTGCTG TGATCTTAAT CCCGATATGC GTCCGTGTTT CGAAACTCTG 
CACGTTTGGC TGCAGCGTCT CGCCGATGAT CTGGCCGCCG ATCGTGTGCC GCCAGAGCGT 
CTGCTGCACG AGATTGAGAC CTTTCAGGAA TGGTACGCCA GCTCTGAGGA CGCCTTGTCA 
CCCACCTCCC AGCGCAGCTT AAATAATCTC GATGAGTTGG TCAAGAGTGC GGTGGATTCG 
GAAATTTCGC CGGTAGAAAA AGAGAAGGAG AATATGGTGA TTAAGCCCCA GGATATACCG 
AAATCTCCGC ATCTCGGCAA GGATTTCTCG CCCAGCGGCG AGAGACTGCG TGACAGTATG 
CGGGCCAGGC GTCGCCAACG TTTCTTGGGC GCCCAGGAGG AGCGTCGTAA TCTCACGCCC 
GACACGGAGT CCAAGGAAAG GGCTTTGAAG AAGGCGCTGA AGAAATGTCG TCCGTTTGGC 
GAAAGAGGTT ACCTGGTGGA TCTGCGTGCC GGTGCCGAAT TGCAGCTAGA GGATGTCAGG 
GATTTGAACA CCTATTCCGA TGTGGACTCC AGTTGCGATA CTAGCCTAAA TTACCATGAT 
GTAAATAATC TGCCAGCAGC GCAAGAGGAT GAGAATACAG TGAAACCGGG CAAGGAAGAA 
CTGTTGGAGG AGAGCACAAA TAAACCCAGC AACCAGGAGT CACAACACCA TCGTCTGGCC 
ATCGATGATA TGCGCACCCG CCTGAATCAG TGCCGCAGCA AATTCGAGCA TTTGGAGGAG 
GCTAGTCGGC GGAACTTCAA CCAGTCGCAG CACTCCATGA AGAACTTTTT CAAAACCCCA 
CCGGTGGCCC TTAAGATGTT TCAGCGCCTG GAGCACGAGG CTGCAGCCCT GAATGGTGGC 
AACAATTGCC CACCGCCACC ACCACGTACG CAGCGCATCA ATCAGACGCC CATTTTCGGT 
CGAAAGAATC CGCCAGTGGC AATCGTCGGG CAAAAACTAC AGCATGCTGA GTCCCTAGAG 
GACCTGGCCT CCAGCGGAGT TGCCAAACAG CTGGCCACAC CAGCACCCAA ACGCAGCAAA 
GCCACAGCAA CGACCAAAGG CGGCCAGAGC AGCAATCCGC CACTTTTCCT GCCGCCTAGC 
CTTAACATAA GTGTCGCATT AAATAGCAAT GGAAATGTGA CCACAACGAC CAATACCAAT 
AGTAGTTGTC CACCATCCGC ATCAGACTGG CTGCCGAAGA AGCACAAGCT AACTTTGCCA 
CTTCCGTCGG CCCAACAGCA GCGAACGAGC AGCAATCATC GCCTGCCAAT GTGCAATAAC 
AAAGGGAAGA CACTGAAGCC ACTGCCAAGT CGCACGGGCA GTCAAGGCAT TCCCGCAAGC 
AATTGCGTCT CGCCGACGAG GAGCAGCAGG CCCGGATCAC CCACCAAGCA TCTGGCCCAG 
AGGCACACAG CCGCCACGGC GCAGCGACTG ACAAATGCGG CCGCCACTCA CCAGCAGCAG 
CATCAGCAAC AATCCTCGAA GACGACGCGC CTGAATATCC TGAGTCCGGA AAAGGTGCAT 
CGCCTGGGCG CTCGACTCAC CGATCAGAAG CAGAAAATGC GCGAGGAGGC CGCAGCGACG 
GCCTCGTCCG TCGGAGGAGC CGGTTGTGCG GCAGGAACAG CGGCTGGAAG CTTAAATGGT 
CATCGGACCA TCGGATCCTC CGGCACACCG AATTCGGCGG TCGGTGAACG GAGGAGGCGA 
GCAGCTCCAT CGCCGCCAGT TCGCACGCAT TTCAATACGC GCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMK1 ( NM_167326.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727621.1 Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01407
Clone ID Related Accession (Same CDS sequence)	NM_001169262.1 , NM_167326.2
Accession Version	NM_167326.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	LIM domain kinase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_167326.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCATC AGCAGCGCCT GCGGGCAAAT GGCGGACGAG GAGGCACTGG ATTGGGCGCA 
GGATCAGGAC CTGTGTCCGG AGGACACTCA CCATTGTGCG CCCATTGTCG GGGTCAACTA 
CTGCCACATC CAGAGGAGCC AATCGTGATG GCTCTCGGCC AGCAATGGCA CTGCGATTGC 
TTTCGCTGCT CCGTTTGCGA GGGCCATCTG CATAACTGGT ACTTCGAACG GGAGGGCTTA 
CTGTACTGTC GTGAGGATTA CTACGGCCGA TTCGGCGATG CCTGTCAGCA GTGTATGGCC 
GTTATCACCG GACCCGTAAT GGTCGCCGGC GAGCACAAAT TCCATCCGGA GTGCTTCTGC 
TGCACGGCAT GCGGCTCCTT CATCGGCGAG GGCGAATCGT ACGCCCTTGT CGAGCGTTCG 
AAGCTCTATT GTGGCCAGTG CTATGGCAAA CGTTCCTGCC AGCCGGCGGA TGCGAAGGCG 
CGGATCACAA CGGCCGGCAA GCCGATGCAC TCCATCCGTC TGGTCGAGAT ACCCAAGGAC 
GCCACGCCCG GCCTGAGGGT CGACGGAGTG GCCCTCGACG ATGGTTGTCC CACGGTCCGC 
ATTACAGAAA TTGATGTGAA TCTTACAAAT CTGCATATTG GAGATAGGAT ATTGGAGGTG 
AACGGCACAC CAGTTAGTGA TTCGTCCGTG GAACAGATCG ACAAATTGAT ACGGAGTAAT 
GAGAAGATGC TTCAGCTGAC CGTGGAGCAT GATCCGGTGC AAGTGTGCCG CTCCTGCAGC 
CAAGCGGACA TCCAGCGGGC CATGTCAGCG TCCACGTTAA TCCTGCCGCT CAGCACATCG 
GCCTCCAGTG TCGAGGTGGG CAGGGAGCGT CTGTACAAGA CACCCGGCGA ACAGGGCACG 
AAGGCCAGGA AACTGCGACA AGCCACCAAT GCCTCTACGA CAATACCACC GGCAGCTGGA 
GCCACCGCAA TGACGCAACT AAAGGAGAAG GAGCGTTGTT CCAGTCTGTC CAAGTTGCTG 
GATGAACAGC ATCAGGCACA ACAGCATTCG GCGCATCCTC AGCTCTATGA TCTGTCCAGG 
ACGCAATCCT GCCGTGTCGT CCAAAAGCCA CAACGAATCT TTCGTGCAAC TGATCTGGTT 
ATAGGAGAGA AGCTAGGCGA GGGCTTCTTT GGCAAGGTTT TCAAGGTGAC CCACCGTCAG 
AGCGGCGAGG TGATGGTCCT CAAAGAACTG CATCGTGCGG ATGAGGAAGC GCAAAGGAAT 
TTCATCAAGG AAGTGGCCGT TCTCCGTTTG CTGGACCATC GGCATGTGCT CAAATTCATT 
GGTGTTCTGT ACAAGGATAA GAAACTGCAC ATGGTCACGG AGTACGTGGC CGGTGGTTGT 
CTCAAGGAGC TGATCCACGA TCCAGCCCAA GTACTCCCTT GGCCCCAGCG CGTCCGCTTG 
GCCAGGGATA TTGCGTGCGG CATGAGCTAT CTGCACTCGA TGAACATCAT CCATCGCGAT 
TTGAACTCAA TGAATTGCCT GGTCCGTGAA GATCGCTCGG TCATAGTGGC GGATTTTGGA 
CTAGCTCGCA GTGTGGATGC GCCGCGGCTG CCAAGTGGCA ATATGACGCC AGGCGGCTAT 
GGATCAGGCG CTAACAGCGA TGCGCCCATG TCACCGAGTG GAACACTAAG GCGCAGCAAG 
AGCAGGCAGC GAAGGCAACG TTACACGGTG GTCGGGAATC CCTACTGGAT GGCACCCGAG 
ATGATGAAAG GTCTCAAATA CGACGAAAAG GTGGATGTCT TCTCCTTTGG CATTATGTTG 
TGTGAGATCA TTGGACGCGT GGAAGCCGAT CCGGATTTTA TGCCTCGCAA TTCGGACTTC 
AGTCTCAATC AGCAGGAGTT TCGCGAGAAG TTCTGCGCTC AGTGTCCAGA GCCTTTCGTT 
AAAGTGGCCT TCGTGTGCTG TGATCTTAAT CCCGATATGC GTCCGTGTTT CGAAACTCTG 
CACGTTTGGC TGCAGCGTCT CGCCGATGAT CTGGCCGCCG ATCGTGTGCC GCCAGAGCGT 
CTGCTGCACG AGATTGAGAC CTTTCAGGAA TGGTACGCCA GCTCTGAGGA CGCCTTGTCA 
CCCACCTCCC AGCGCAGCTT AAATAATCTC GATGAGTTGG TCAAGAGTGC GGTGGATTCG 
GAAATTTCGC CGGTAGAAAA AGAGAAGGAG AATATGGTGA TTAAGCCCCA GGATATACCG 
AAATCTCCGC ATCTCGGCAA GGATTTCTCG CCCAGCGGCG AGAGACTGCG TGACAGTATG 
CGGGCCAGGC GTCGCCAACG TTTCTTGGGC GCCCAGGAGG AGCGTCGTAA TCTCACGCCC 
GACACGGAGT CCAAGGAAAG GGCTTTGAAG AAGGCGCTGA AGAAATGTCG TCCGTTTGGC 
GAAAGAGGTT ACCTGGTGGA TCTGCGTGCC GGTGCCGAAT TGCAGCTAGA GGATGTCAGG 
GATTTGAACA CCTATTCCGA TGTGGACTCC AGTTGCGATA CTAGCCTAAA TTACCATGAT 
GTAAATAATC TGCCAGCAGC GCAAGAGGAT GAGAATACAG TGAAACCGGG CAAGGAAGAA 
CTGTTGGAGG AGAGCACAAA TAAACCCAGC AACCAGGAGT CACAACACCA TCGTCTGGCC 
ATCGATGATA TGCGCACCCG CCTGAATCAG TGCCGCAGCA AATTCGAGCA TTTGGAGGAG 
GCTAGTCGGC GGAACTTCAA CCAGTCGCAG CACTCCATGA AGAACTTTTT CAAAACCCCA 
CCGGTGGCCC TTAAGATGTT TCAGCGCCTG GAGCACGAGG CTGCAGCCCT GAATGGTGGC 
AACAATTGCC CACCGCCACC ACCACGTACG CAGCGCATCA ATCAGACGCC CATTTTCGGT 
CGAAAGAATC CGCCAGTGGC AATCGTCGGG CAAAAACTAC AGCATGCTGA GTCCCTAGAG 
GACCTGGCCT CCAGCGGAGT TGCCAAACAG CTGGCCACAC CAGCACCCAA ACGCAGCAAA 
GCCACAGCAA CGACCAAAGG CGGCCAGAGC AGCAATCCGC CACTTTTCCT GCCGCCTAGC 
CTTAACATAA GTGTCGCATT AAATAGCAAT GGAAATGTGA CCACAACGAC CAATACCAAT 
AGTAGTTGTC CACCATCCGC ATCAGACTGG CTGCCGAAGA AGCACAAGCT AACTTTGCCA 
CTTCCGTCGG CCCAACAGCA GCGAACGAGC AGCAATCATC GCCTGCCAAT GTGCAATAAC 
AAAGGGAAGA CACTGAAGCC ACTGCCAAGT CGCACGGGCA GTCAAGGCAT TCCCGCAAGC 
AATTGCGTCT CGCCGACGAG GAGCAGCAGG CCCGGATCAC CCACCAAGCA TCTGGCCCAG 
AGGCACACAG CCGCCACGGC GCAGCGACTG ACAAATGCGG CCGCCACTCA CCAGCAGCAG 
CATCAGCAAC AATCCTCGAA GACGACGCGC CTGAATATCC TGAGTCCGGA AAAGGTGCAT 
CGCCTGGGCG CTCGACTCAC CGATCAGAAG CAGAAAATGC GCGAGGAGGC CGCAGCGACG 
GCCTCGTCCG TCGGAGGAGC CGGTTGTGCG GCAGGAACAG CGGCTGGAAG CTTAAATGGT 
CATCGGACCA TCGGATCCTC CGGCACACCG AATTCGGCGG TCGGTGAACG GAGGAGGCGA 
GCAGCTCCAT CGCCGCCAGT TCGCACGCAT TTCAATACGC GCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727621.1
CDS	263..3970
Translation	MHHQQRLRANGGRGGTGLGAGSGPVSGGHSPLCAHCRGQLLPHPEEPIVMALGQQWHCDCFRCSVCEGHLHNWYFEREGLLYCREDYYGRFGDACQQCMAVITGPVMVAGEHKFHPECFCCTACGSFIGEGESYALVERSKLYCGQCYGKRSCQPADAKARITTAGKPMHSIRLVEIPKDATPGLRVDGVALDDGCPTVRITEIDVNLTNLHIGDRILEVNGTPVSDSSVEQIDKLIRSNEKMLQLTVEHDPVQVCRSCSQADIQRAMSASTLILPLSTSASSVEVGRERLYKTPGEQGTKARKLRQATNASTTIPPAAGATAMTQLKEKERCSSLSKLLDEQHQAQQHSAHPQLYDLSRTQSCRVVQKPQRIFRATDLVIGEKLGEGFFGKVFKVTHRQSGEVMVLKELHRADEEAQRNFIKEVAVLRLLDHRHVLKFIGVLYKDKKLHMVTEYVAGGCLKELIHDPAQVLPWPQRVRLARDIACGMSYLHSMNIIHRDLNSMNCLVREDRSVIVADFGLARSVDAPRLPSGNMTPGGYGSGANSDAPMSPSGTLRRSKSRQRRQRYTVVGNPYWMAPEMMKGLKYDEKVDVFSFGIMLCEIIGRVEADPDFMPRNSDFSLNQQEFREKFCAQCPEPFVKVAFVCCDLNPDMRPCFETLHVWLQRLADDLAADRVPPERLLHEIETFQEWYASSEDALSPTSQRSLNNLDELVKSAVDSEISPVEKEKENMVIKPQDIPKSPHLGKDFSPSGERLRDSMRARRRQRFLGAQEERRNLTPDTESKERALKKALKKCRPFGERGYLVDLRAGAELQLEDVRDLNTYSDVDSSCDTSLNYHDVNNLPAAQEDENTVKPGKEELLEESTNKPSNQESQHHRLAIDDMRTRLNQCRSKFEHLEEASRRNFNQSQHSMKNFFKTPPVALKMFQRLEHEAAALNGGNNCPPPPPRTQRINQTPIFGRKNPPVAIVGQKLQHAESLEDLASSGVAKQLATPAPKRSKATATTKGGQSSNPPLFLPPSLNISVALNSNGNVTTTTNTNSSCPPSASDWLPKKHKLTLPLPSAQQQRTSSNHRLPMCNNKGKTLKPLPSRTGSQGIPASNCVSPTRSSRPGSPTKHLAQRHTAATAQRLTNAAATHQQQHQQQSSKTTRLNILSPEKVHRLGARLTDQKQKMREEAAATASSVGGAGCAAGTAAGSLNGHRTIGSSGTPNSAVGERRRRAAPSPPVRTHFNTRC

Target ORF information:

RefSeq Version	NM_167326.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167326.2

ORF Insert Sequence:

ATGCATCATC AGCAGCGCCT GCGGGCAAAT GGCGGACGAG GAGGCACTGG ATTGGGCGCA 
GGATCAGGAC CTGTGTCCGG AGGACACTCA CCATTGTGCG CCCATTGTCG GGGTCAACTA 
CTGCCACATC CAGAGGAGCC AATCGTGATG GCTCTCGGCC AGCAATGGCA CTGCGATTGC 
TTTCGCTGCT CCGTTTGCGA GGGCCATCTG CATAACTGGT ACTTCGAACG GGAGGGCTTA 
CTGTACTGTC GTGAGGATTA CTACGGCCGA TTCGGCGATG CCTGTCAGCA GTGTATGGCC 
GTTATCACCG GACCCGTAAT GGTCGCCGGC GAGCACAAAT TCCATCCGGA GTGCTTCTGC 
TGCACGGCAT GCGGCTCCTT CATCGGCGAG GGCGAATCGT ACGCCCTTGT CGAGCGTTCG 
AAGCTCTATT GTGGCCAGTG CTATGGCAAA CGTTCCTGCC AGCCGGCGGA TGCGAAGGCG 
CGGATCACAA CGGCCGGCAA GCCGATGCAC TCCATCCGTC TGGTCGAGAT ACCCAAGGAC 
GCCACGCCCG GCCTGAGGGT CGACGGAGTG GCCCTCGACG ATGGTTGTCC CACGGTCCGC 
ATTACAGAAA TTGATGTGAA TCTTACAAAT CTGCATATTG GAGATAGGAT ATTGGAGGTG 
AACGGCACAC CAGTTAGTGA TTCGTCCGTG GAACAGATCG ACAAATTGAT ACGGAGTAAT 
GAGAAGATGC TTCAGCTGAC CGTGGAGCAT GATCCGGTGC AAGTGTGCCG CTCCTGCAGC 
CAAGCGGACA TCCAGCGGGC CATGTCAGCG TCCACGTTAA TCCTGCCGCT CAGCACATCG 
GCCTCCAGTG TCGAGGTGGG CAGGGAGCGT CTGTACAAGA CACCCGGCGA ACAGGGCACG 
AAGGCCAGGA AACTGCGACA AGCCACCAAT GCCTCTACGA CAATACCACC GGCAGCTGGA 
GCCACCGCAA TGACGCAACT AAAGGAGAAG GAGCGTTGTT CCAGTCTGTC CAAGTTGCTG 
GATGAACAGC ATCAGGCACA ACAGCATTCG GCGCATCCTC AGCTCTATGA TCTGTCCAGG 
ACGCAATCCT GCCGTGTCGT CCAAAAGCCA CAACGAATCT TTCGTGCAAC TGATCTGGTT 
ATAGGAGAGA AGCTAGGCGA GGGCTTCTTT GGCAAGGTTT TCAAGGTGAC CCACCGTCAG 
AGCGGCGAGG TGATGGTCCT CAAAGAACTG CATCGTGCGG ATGAGGAAGC GCAAAGGAAT 
TTCATCAAGG AAGTGGCCGT TCTCCGTTTG CTGGACCATC GGCATGTGCT CAAATTCATT 
GGTGTTCTGT ACAAGGATAA GAAACTGCAC ATGGTCACGG AGTACGTGGC CGGTGGTTGT 
CTCAAGGAGC TGATCCACGA TCCAGCCCAA GTACTCCCTT GGCCCCAGCG CGTCCGCTTG 
GCCAGGGATA TTGCGTGCGG CATGAGCTAT CTGCACTCGA TGAACATCAT CCATCGCGAT 
TTGAACTCAA TGAATTGCCT GGTCCGTGAA GATCGCTCGG TCATAGTGGC GGATTTTGGA 
CTAGCTCGCA GTGTGGATGC GCCGCGGCTG CCAAGTGGCA ATATGACGCC AGGCGGCTAT 
GGATCAGGCG CTAACAGCGA TGCGCCCATG TCACCGAGTG GAACACTAAG GCGCAGCAAG 
AGCAGGCAGC GAAGGCAACG TTACACGGTG GTCGGGAATC CCTACTGGAT GGCACCCGAG 
ATGATGAAAG GTCTCAAATA CGACGAAAAG GTGGATGTCT TCTCCTTTGG CATTATGTTG 
TGTGAGATCA TTGGACGCGT GGAAGCCGAT CCGGATTTTA TGCCTCGCAA TTCGGACTTC 
AGTCTCAATC AGCAGGAGTT TCGCGAGAAG TTCTGCGCTC AGTGTCCAGA GCCTTTCGTT 
AAAGTGGCCT TCGTGTGCTG TGATCTTAAT CCCGATATGC GTCCGTGTTT CGAAACTCTG 
CACGTTTGGC TGCAGCGTCT CGCCGATGAT CTGGCCGCCG ATCGTGTGCC GCCAGAGCGT 
CTGCTGCACG AGATTGAGAC CTTTCAGGAA TGGTACGCCA GCTCTGAGGA CGCCTTGTCA 
CCCACCTCCC AGCGCAGCTT AAATAATCTC GATGAGTTGG TCAAGAGTGC GGTGGATTCG 
GAAATTTCGC CGGTAGAAAA AGAGAAGGAG AATATGGTGA TTAAGCCCCA GGATATACCG 
AAATCTCCGC ATCTCGGCAA GGATTTCTCG CCCAGCGGCG AGAGACTGCG TGACAGTATG 
CGGGCCAGGC GTCGCCAACG TTTCTTGGGC GCCCAGGAGG AGCGTCGTAA TCTCACGCCC 
GACACGGAGT CCAAGGAAAG GGCTTTGAAG AAGGCGCTGA AGAAATGTCG TCCGTTTGGC 
GAAAGAGGTT ACCTGGTGGA TCTGCGTGCC GGTGCCGAAT TGCAGCTAGA GGATGTCAGG 
GATTTGAACA CCTATTCCGA TGTGGACTCC AGTTGCGATA CTAGCCTAAA TTACCATGAT 
GTAAATAATC TGCCAGCAGC GCAAGAGGAT GAGAATACAG TGAAACCGGG CAAGGAAGAA 
CTGTTGGAGG AGAGCACAAA TAAACCCAGC AACCAGGAGT CACAACACCA TCGTCTGGCC 
ATCGATGATA TGCGCACCCG CCTGAATCAG TGCCGCAGCA AATTCGAGCA TTTGGAGGAG 
GCTAGTCGGC GGAACTTCAA CCAGTCGCAG CACTCCATGA AGAACTTTTT CAAAACCCCA 
CCGGTGGCCC TTAAGATGTT TCAGCGCCTG GAGCACGAGG CTGCAGCCCT GAATGGTGGC 
AACAATTGCC CACCGCCACC ACCACGTACG CAGCGCATCA ATCAGACGCC CATTTTCGGT 
CGAAAGAATC CGCCAGTGGC AATCGTCGGG CAAAAACTAC AGCATGCTGA GTCCCTAGAG 
GACCTGGCCT CCAGCGGAGT TGCCAAACAG CTGGCCACAC CAGCACCCAA ACGCAGCAAA 
GCCACAGCAA CGACCAAAGG CGGCCAGAGC AGCAATCCGC CACTTTTCCT GCCGCCTAGC 
CTTAACATAA GTGTCGCATT AAATAGCAAT GGAAATGTGA CCACAACGAC CAATACCAAT 
AGTAGTTGTC CACCATCCGC ATCAGACTGG CTGCCGAAGA AGCACAAGCT AACTTTGCCA 
CTTCCGTCGG CCCAACAGCA GCGAACGAGC AGCAATCATC GCCTGCCAAT GTGCAATAAC 
AAAGGGAAGA CACTGAAGCC ACTGCCAAGT CGCACGGGCA GTCAAGGCAT TCCCGCAAGC 
AATTGCGTCT CGCCGACGAG GAGCAGCAGG CCCGGATCAC CCACCAAGCA TCTGGCCCAG 
AGGCACACAG CCGCCACGGC GCAGCGACTG ACAAATGCGG CCGCCACTCA CCAGCAGCAG 
CATCAGCAAC AATCCTCGAA GACGACGCGC CTGAATATCC TGAGTCCGGA AAAGGTGCAT 
CGCCTGGGCG CTCGACTCAC CGATCAGAAG CAGAAAATGC GCGAGGAGGC CGCAGCGACG 
GCCTCGTCCG TCGGAGGAGC CGGTTGTGCG GCAGGAACAG CGGCTGGAAG CTTAAATGGT 
CATCGGACCA TCGGATCCTC CGGCACACCG AATTCGGCGG TCGGTGAACG GAGGAGGCGA 
GCAGCTCCAT CGCCGCCAGT TCGCACGCAT TTCAATACGC GCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMK1 ( NM_001169263.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162734.1 Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa02798
Clone ID Related Accession (Same CDS sequence)	NM_001169263.1
Accession Version	NM_001169263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	LIM domain kinase 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAAC AGTGCTGTGG GGCGCCTTAT AGAATTGATG TGAATCTTAC AAATCTGCAT 
ATTGGAGATA GGATATTGGA GGTGAACGGC ACACCAGTTA GTGATTCGTC CGTGGAACAG 
ATCGACAAAT TGATACGGAG TAATGAGAAG ATGCTTCAGC TGACCGTGGA GCATGATCCG 
GTGCAAGTGT GCCGCTCCTG CAGCCAAGCG GACATCCAGC GGGCCATGTC AGCGTCCACG 
TTAATCCTGC CGCTCAGCAC ATCGGCCTCC AGTGTCGAGG TGGGCAGGGA GCGTCTGTAC 
AAGACACCCG GCGAACAGGG CACGAAGGCC AGGAAACTGC GACAAGCCAC CAATGCCTCT 
ACGACAATAC CACCGGCAGC TGGAGCCACC GCAATGACGC AACTAAAGGA GAAGGAGCGT 
TGTTCCAGTC TGTCCAAGTT GCTGGATGAA CAGCATCAGG CACAACAGCA TTCGGCGCAT 
CCTCAGCTCT ATGATCTGTC CAGGACGCAA TCCTGCCGTG TCGTCCAAAA GCCACAACGA 
ATCTTTCGTG CAACTGATCT GGTTATAGGA GAGAAGCTAG GCGAGGGCTT CTTTGGCAAG 
GTTTTCAAGG TGACCCACCG TCAGAGCGGC GAGGTGATGG TCCTCAAAGA ACTGCATCGT 
GCGGATGAGG AAGCGCAAAG GAATTTCATC AAGGAAGTGG CCGTTCTCCG TTTGCTGGAC 
CATCGGCATG TGCTCAAATT CATTGGTGTT CTGTACAAGG ATAAGAAACT GCACATGGTC 
ACGGAGTACG TGGCCGGTGG TTGTCTCAAG GAGCTGATCC ACGATCCAGC CCAAGTACTC 
CCTTGGCCCC AGCGCGTCCG CTTGGCCAGG GATATTGCGT GCGGCATGAG CTATCTGCAC 
TCGATGAACA TCATCCATCG CGATTTGAAC TCAATGAATT GCCTGGTCCG TGAAGATCGC 
TCGGTCATAG TGGCGGATTT TGGACTAGCT CGCAGTGTGG ATGCGCCGCG GCTGCCAAGT 
GGCAATATGA CGCCAGGCGG CTATGGATCA GGCGCTAACA GCGATGCGCC CATGTCACCG 
AGTGGAACAC TAAGGCGCAG CAAGAGCAGG CAGCGAAGGC AACGTTACAC GGTGGTCGGG 
AATCCCTACT GGATGGCACC CGAGATGATG AAAGGTCTCA AATACGACGA AAAGGTGGAT 
GTCTTCTCCT TTGGCATTAT GTTGTGTGAG ATCATTGGAC GCGTGGAAGC CGATCCGGAT 
TTTATGCCTC GCAATTCGGA CTTCAGTCTC AATCAGCAGG AGTTTCGCGA GAAGTTCTGC 
GCTCAGTGTC CAGAGCCTTT CGTTAAAGTG GCCTTCGTGT GCTGTGATCT TAATCCCGAT 
ATGCGTCCGT GTTTCGAAAC TCTGCACGTT TGGCTGCAGC GTCTCGCCGA TGATCTGGCC 
GCCGATCGTG TGCCGCCAGA GCGTCTGCTG CACGAGATTG AGACCTTTCA GGAATGGTAC 
GCCAGCTCTG AGGACGCCTT GTCACCCACC TCCCAGCGCA GCTTAAATAA TCTCGATGAG 
TTGGTCAAGA GTGCGGTGGA TTCGGAAATT TCGCCGGTAG AAAAAGAGAA GGAGAATATG 
GTGATTAAGC CCCAGGATAT ACCGAAATCT CCGCATCTCG GCAAGGATTT CTCGCCCAGC 
GGCGAGAGAC TGCGTGACAG TATGCGGGCC AGGCGTCGCC AACGTTTCTT GGGCGCCCAG 
GAGGAGCGTC GTAATCTCAC GCCCGACACG GAGTCCAAGG AAAGGGCTTT GAAGAAGGCG 
CTGAAGAAAT GTCGTCCGTT TGGCGAAAGA GGTTACCTGG TGGATCTGCG TGCCGGTGCC 
GAATTGCAGC TAGAGGATGT CAGGGATTTG AACACCTATT CCGATGTGGA CTCCAGTTGC 
GATACTAGCC TAAATTACCA TGATGTAAAT AATCTGCCAG CAGCGCAAGA GGATGAGAAT 
ACAGTGAAAC CGGGCAAGGA AGAACTGTTG GAGGAGAGCA CAAATAAACC CAGCAACCAG 
GAGTCACAAC ACCATCGTCT GGCCATCGAT GATATGCGCA CCCGCCTGAA TCAGTGCCGC 
AGCAAATTCG AGCATTTGGA GGAGGCTAGT CGGCGGAACT TCAACCAGTC GCAGCACTCC 
ATGAAGAACT TTTTCAAAAC CCCACCGGTG GCCCTTAAGA TGTTTCAGCG CCTGGAGCAC 
GAGGCTGCAG CCCTGAATGG TGGCAACAAT TGCCCACCGC CACCACCACG TACGCAGCGC 
ATCAATCAGA CGCCCATTTT CGGTCGAAAG AATCCGCCAG TGGCAATCGT CGGGCAAAAA 
CTACAGCATG CTGAGTCCCT AGAGGACCTG GCCTCCAGCG GAGTTGCCAA ACAGCTGGCC 
ACACCAGCAC CCAAACGCAG CAAAGCCACA GCAACGACCA AAGGCGGCCA GAGCAGCAAT 
CCGCCACTTT TCCTGCCGCC TAGCCTTAAC ATAAGTGTCG CATTAAATAG CAATGGAAAT 
GTGACCACAA CGACCAATAC CAATAGTAGT TGTCCACCAT CCGCATCAGA CTGGCTGCCG 
AAGAAGCACA AGCTAACTTT GCCACTTCCG TCGGCCCAAC AGCAGCGAAC GAGCAGCAAT 
CATCGCCTGC CAATGTGCAA TAACAAAGGG AAGACACTGA AGCCACTGCC AAGTCGCACG 
GGCAGTCAAG GCATTCCCGC AAGCAATTGC GTCTCGCCGA CGAGGAGCAG CAGGCCCGGA 
TCACCCACCA AGCATCTGGC CCAGAGGCAC ACAGCCGCCA CGGCGCAGCG ACTGACAAAT 
GCGGCCGCCA CTCACCAGCA GCAGCATCAG CAACAATCCT CGAAGACGAC GCGCCTGAAT 
ATCCTGAGTC CGGAAAAGGT GCATCGCCTG GGCGCTCGAC TCACCGATCA GAAGCAGAAA 
ATGCGCGAGG AGGCCGCAGC GACGGCCTCG TCCGTCGGAG GAGCCGGTTG TGCGGCAGGA 
ACAGCGGCTG GAAGCTTAAA TGGTCATCGG ACCATCGGAT CCTCCGGCAC ACCGAATTCG 
GCGGTCGGTG AACGGAGGAG GCGAGCAGCT CCATCGCCGC CAGTTCGCAC GCATTTCAAT 
ACGCGCTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162734.1
CDS	62..3193
Translation	MAEQCCGAPYRIDVNLTNLHIGDRILEVNGTPVSDSSVEQIDKLIRSNEKMLQLTVEHDPVQVCRSCSQADIQRAMSASTLILPLSTSASSVEVGRERLYKTPGEQGTKARKLRQATNASTTIPPAAGATAMTQLKEKERCSSLSKLLDEQHQAQQHSAHPQLYDLSRTQSCRVVQKPQRIFRATDLVIGEKLGEGFFGKVFKVTHRQSGEVMVLKELHRADEEAQRNFIKEVAVLRLLDHRHVLKFIGVLYKDKKLHMVTEYVAGGCLKELIHDPAQVLPWPQRVRLARDIACGMSYLHSMNIIHRDLNSMNCLVREDRSVIVADFGLARSVDAPRLPSGNMTPGGYGSGANSDAPMSPSGTLRRSKSRQRRQRYTVVGNPYWMAPEMMKGLKYDEKVDVFSFGIMLCEIIGRVEADPDFMPRNSDFSLNQQEFREKFCAQCPEPFVKVAFVCCDLNPDMRPCFETLHVWLQRLADDLAADRVPPERLLHEIETFQEWYASSEDALSPTSQRSLNNLDELVKSAVDSEISPVEKEKENMVIKPQDIPKSPHLGKDFSPSGERLRDSMRARRRQRFLGAQEERRNLTPDTESKERALKKALKKCRPFGERGYLVDLRAGAELQLEDVRDLNTYSDVDSSCDTSLNYHDVNNLPAAQEDENTVKPGKEELLEESTNKPSNQESQHHRLAIDDMRTRLNQCRSKFEHLEEASRRNFNQSQHSMKNFFKTPPVALKMFQRLEHEAAALNGGNNCPPPPPRTQRINQTPIFGRKNPPVAIVGQKLQHAESLEDLASSGVAKQLATPAPKRSKATATTKGGQSSNPPLFLPPSLNISVALNSNGNVTTTTNTNSSCPPSASDWLPKKHKLTLPLPSAQQQRTSSNHRLPMCNNKGKTLKPLPSRTGSQGIPASNCVSPTRSSRPGSPTKHLAQRHTAATAQRLTNAAATHQQQHQQQSSKTTRLNILSPEKVHRLGARLTDQKQKMREEAAATASSVGGAGCAAGTAAGSLNGHRTIGSSGTPNSAVGERRRRAAPSPPVRTHFNTRC

Target ORF information:

RefSeq Version	NM_001169263.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169263.1

ORF Insert Sequence:

ATGGCGGAAC AGTGCTGTGG GGCGCCTTAT AGAATTGATG TGAATCTTAC AAATCTGCAT 
ATTGGAGATA GGATATTGGA GGTGAACGGC ACACCAGTTA GTGATTCGTC CGTGGAACAG 
ATCGACAAAT TGATACGGAG TAATGAGAAG ATGCTTCAGC TGACCGTGGA GCATGATCCG 
GTGCAAGTGT GCCGCTCCTG CAGCCAAGCG GACATCCAGC GGGCCATGTC AGCGTCCACG 
TTAATCCTGC CGCTCAGCAC ATCGGCCTCC AGTGTCGAGG TGGGCAGGGA GCGTCTGTAC 
AAGACACCCG GCGAACAGGG CACGAAGGCC AGGAAACTGC GACAAGCCAC CAATGCCTCT 
ACGACAATAC CACCGGCAGC TGGAGCCACC GCAATGACGC AACTAAAGGA GAAGGAGCGT 
TGTTCCAGTC TGTCCAAGTT GCTGGATGAA CAGCATCAGG CACAACAGCA TTCGGCGCAT 
CCTCAGCTCT ATGATCTGTC CAGGACGCAA TCCTGCCGTG TCGTCCAAAA GCCACAACGA 
ATCTTTCGTG CAACTGATCT GGTTATAGGA GAGAAGCTAG GCGAGGGCTT CTTTGGCAAG 
GTTTTCAAGG TGACCCACCG TCAGAGCGGC GAGGTGATGG TCCTCAAAGA ACTGCATCGT 
GCGGATGAGG AAGCGCAAAG GAATTTCATC AAGGAAGTGG CCGTTCTCCG TTTGCTGGAC 
CATCGGCATG TGCTCAAATT CATTGGTGTT CTGTACAAGG ATAAGAAACT GCACATGGTC 
ACGGAGTACG TGGCCGGTGG TTGTCTCAAG GAGCTGATCC ACGATCCAGC CCAAGTACTC 
CCTTGGCCCC AGCGCGTCCG CTTGGCCAGG GATATTGCGT GCGGCATGAG CTATCTGCAC 
TCGATGAACA TCATCCATCG CGATTTGAAC TCAATGAATT GCCTGGTCCG TGAAGATCGC 
TCGGTCATAG TGGCGGATTT TGGACTAGCT CGCAGTGTGG ATGCGCCGCG GCTGCCAAGT 
GGCAATATGA CGCCAGGCGG CTATGGATCA GGCGCTAACA GCGATGCGCC CATGTCACCG 
AGTGGAACAC TAAGGCGCAG CAAGAGCAGG CAGCGAAGGC AACGTTACAC GGTGGTCGGG 
AATCCCTACT GGATGGCACC CGAGATGATG AAAGGTCTCA AATACGACGA AAAGGTGGAT 
GTCTTCTCCT TTGGCATTAT GTTGTGTGAG ATCATTGGAC GCGTGGAAGC CGATCCGGAT 
TTTATGCCTC GCAATTCGGA CTTCAGTCTC AATCAGCAGG AGTTTCGCGA GAAGTTCTGC 
GCTCAGTGTC CAGAGCCTTT CGTTAAAGTG GCCTTCGTGT GCTGTGATCT TAATCCCGAT 
ATGCGTCCGT GTTTCGAAAC TCTGCACGTT TGGCTGCAGC GTCTCGCCGA TGATCTGGCC 
GCCGATCGTG TGCCGCCAGA GCGTCTGCTG CACGAGATTG AGACCTTTCA GGAATGGTAC 
GCCAGCTCTG AGGACGCCTT GTCACCCACC TCCCAGCGCA GCTTAAATAA TCTCGATGAG 
TTGGTCAAGA GTGCGGTGGA TTCGGAAATT TCGCCGGTAG AAAAAGAGAA GGAGAATATG 
GTGATTAAGC CCCAGGATAT ACCGAAATCT CCGCATCTCG GCAAGGATTT CTCGCCCAGC 
GGCGAGAGAC TGCGTGACAG TATGCGGGCC AGGCGTCGCC AACGTTTCTT GGGCGCCCAG 
GAGGAGCGTC GTAATCTCAC GCCCGACACG GAGTCCAAGG AAAGGGCTTT GAAGAAGGCG 
CTGAAGAAAT GTCGTCCGTT TGGCGAAAGA GGTTACCTGG TGGATCTGCG TGCCGGTGCC 
GAATTGCAGC TAGAGGATGT CAGGGATTTG AACACCTATT CCGATGTGGA CTCCAGTTGC 
GATACTAGCC TAAATTACCA TGATGTAAAT AATCTGCCAG CAGCGCAAGA GGATGAGAAT 
ACAGTGAAAC CGGGCAAGGA AGAACTGTTG GAGGAGAGCA CAAATAAACC CAGCAACCAG 
GAGTCACAAC ACCATCGTCT GGCCATCGAT GATATGCGCA CCCGCCTGAA TCAGTGCCGC 
AGCAAATTCG AGCATTTGGA GGAGGCTAGT CGGCGGAACT TCAACCAGTC GCAGCACTCC 
ATGAAGAACT TTTTCAAAAC CCCACCGGTG GCCCTTAAGA TGTTTCAGCG CCTGGAGCAC 
GAGGCTGCAG CCCTGAATGG TGGCAACAAT TGCCCACCGC CACCACCACG TACGCAGCGC 
ATCAATCAGA CGCCCATTTT CGGTCGAAAG AATCCGCCAG TGGCAATCGT CGGGCAAAAA 
CTACAGCATG CTGAGTCCCT AGAGGACCTG GCCTCCAGCG GAGTTGCCAA ACAGCTGGCC 
ACACCAGCAC CCAAACGCAG CAAAGCCACA GCAACGACCA AAGGCGGCCA GAGCAGCAAT 
CCGCCACTTT TCCTGCCGCC TAGCCTTAAC ATAAGTGTCG CATTAAATAG CAATGGAAAT 
GTGACCACAA CGACCAATAC CAATAGTAGT TGTCCACCAT CCGCATCAGA CTGGCTGCCG 
AAGAAGCACA AGCTAACTTT GCCACTTCCG TCGGCCCAAC AGCAGCGAAC GAGCAGCAAT 
CATCGCCTGC CAATGTGCAA TAACAAAGGG AAGACACTGA AGCCACTGCC AAGTCGCACG 
GGCAGTCAAG GCATTCCCGC AAGCAATTGC GTCTCGCCGA CGAGGAGCAG CAGGCCCGGA 
TCACCCACCA AGCATCTGGC CCAGAGGCAC ACAGCCGCCA CGGCGCAGCG ACTGACAAAT 
GCGGCCGCCA CTCACCAGCA GCAGCATCAG CAACAATCCT CGAAGACGAC GCGCCTGAAT 
ATCCTGAGTC CGGAAAAGGT GCATCGCCTG GGCGCTCGAC TCACCGATCA GAAGCAGAAA 
ATGCGCGAGG AGGCCGCAGC GACGGCCTCG TCCGTCGGAG GAGCCGGTTG TGCGGCAGGA 
ACAGCGGCTG GAAGCTTAAA TGGTCATCGG ACCATCGGAT CCTCCGGCAC ACCGAATTCG 
GCGGTCGGTG AACGGAGGAG GCGAGCAGCT CCATCGCCGC CAGTTCGCAC GCATTTCAAT 
ACGCGCTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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LIMK1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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